LOCUS       DAUCLR010000001        48090 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.001, whole genome shotgun sequence.
ACCESSION   DAUCLR010000001 DAUCLR010000000
VERSION     DAUCLR010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 48090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48090
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.001"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..332
                     /locus_tag="VGT87_000001"
     CDS             <1..332
                     /locus_tag="VGT87_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3812898.1"
                     /translation="TTKNFRSGQKVWFLTEHGLYEVLMRSNKPKAKEFRKAVKNILKE
                     IRLNGYYMQGELVQELAQSSTQKLPGISDLTYIRNKLADLVDMDNLADISNGIDRVQQ
                     LVKLISL"
     gene            342..959
                     /locus_tag="VGT87_000002"
     CDS             342..959
                     /locus_tag="VGT87_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HEQ3812899.1"
                     /translation="MNKTETWNGYTIRFVEHQGEWWAVAKDITNALGLKQPSRAISTL
                     KGVTKSKTLTKGGEQELSIINEKDIYRLVFKSRKPEAEAFQEWVFETIKQLRQSTGLE
                     GFQVFRMFDKEHQKQSMNRLVNGLQNATKKDLIKANTIANKAVSNKYGLPKMISKNEM
                     TENMLRDREPILDETVELIKVKEKYGLNFSVSEAIFNQNTIKKAQ"
     gene            986..1228
                     /locus_tag="VGT87_000003"
     CDS             986..1228
                     /locus_tag="VGT87_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor protein"
                     /protein_id="HEQ3812900.1"
                     /translation="MKATTYKELKKWIDEGVDLAELAQGYADKVPNADREQFEAITQE
                     IFNVLEGVSLMLDDKVLIYNRKAEQKRLNDIEQGNY"
     gene            1485..1817
                     /locus_tag="VGT87_000004"
     CDS             1485..1817
                     /locus_tag="VGT87_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3812901.1"
                     /translation="MKYRVETNPFSKDRYTPEQLEMFKNRQLSKNKAEAYFTRLYNQH
                     IAWVIIANVMTEYVIKFRKSATSFEEAWDALDYQRTTEIVFRAVNGLPCSEKDSGELE
                     TYLSEVSA"
     gene            1817..2008
                     /locus_tag="VGT87_000005"
     CDS             1817..2008
                     /locus_tag="VGT87_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3812902.1"
                     /translation="MQELNLTPTQTLILFFVLSLLGLLLSLSKSLIDIDLPEDTQVPK
                     PPKNANYGAYIQSQNHYYN"
     gene            2020..2382
                     /locus_tag="VGT87_000006"
     CDS             2020..2382
                     /locus_tag="VGT87_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3812903.1"
                     /translation="MTTEEYFKKLLEDSEKQPTNWLSDRVLDKNIKMLDEDIEAEWDD
                     FPLFTKKIFINTTQRNYNKADAVVKFLESIPIEMEYKKGLNIVIQALNELKDSLKNQV
                     LGVIDNQILFPNDKEDTK"
     gene            2379..2687
                     /locus_tag="VGT87_000007"
     CDS             2379..2687
                     /locus_tag="VGT87_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3812904.1"
                     /translation="MTLPENYRRVLNLIKVGAEISLILKLEERSVQSIISSLITRYNV
                     PIIGIRHGFNRGYFIPANKEELLDGAKAFYNQVQKEQERLSVLLNADLTSYKELLKGG
                     "
     gene            2690..2962
                     /locus_tag="VGT87_000008"
     CDS             2690..2962
                     /locus_tag="VGT87_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3812905.1"
                     /translation="MNVFSQDYESKLLEQNLTAFNRFLEAYQKPKPRVLGLITAEQVK
                     EELNIKGKTLKRWENAGLRRYQPPLEDTRKHYYKVSDILIFLGVSV"
     gene            2963..3829
                     /locus_tag="VGT87_000009"
     CDS             2963..3829
                     /locus_tag="VGT87_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="HEQ3812906.1"
                     /translation="MAIYEARGFSSYLYPYKGPLEPFDYIAQFRPLKPPEDIDIEEYK
                     RTQAPYCLSGKVTAEKNGSYKRNNASLVYRDLIFLDYDEIETGVNLPKIVSQTLWEYS
                     YIIYPTIKHTPEKPRYRLVMKPSDVMTEATYKQVVKEIADKIGLPFDLASLTWSQLQG
                     LPITTGDPEDYQRYVNRGLGYPVPKNGNTPNRQVVTTYTPRPRSQRSITMRVIDTLFN
                     GFGDEGGRNVALTKFVGLLFNKWVDCDLETAYELVQIANSVTTKPLPIDEIDTTFRSI
                     LDKELRKRGIKP"
     gene            3841..5234
                     /locus_tag="VGT87_000010"
                     /pseudo
     CDS             3841..5234
                     /locus_tag="VGT87_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656427.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated protein E"
     gene            5528..5782
                     /locus_tag="VGT87_000011"
     CDS             5528..5782
                     /locus_tag="VGT87_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3812907.1"
                     /translation="MNPNYTNHGVKKMRYNYDDLPNDQLYRLELPNGQCGYDFITKIN
                     FNTKSRPGDCDFTRPELTRRGYQWVFDCGFAIPVEKGAKT"
     gene            5779..5952
                     /locus_tag="VGT87_000012"
     CDS             5779..5952
                     /locus_tag="VGT87_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2758 domain-containing protein"
                     /protein_id="HEQ3812908.1"
                     /translation="MKIKLFYQKHNESLDDFEHRVNLFTLSVSVIDIKFQEATYGNYE
                     DMDSRTGLLVLYR"
     gene            5958..6131
                     /locus_tag="VGT87_000013"
     CDS             5958..6131
                     /locus_tag="VGT87_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3812909.1"
                     /translation="MKLKLHTRGGNTITIQGDRTLYDELTKYLLSGQQPNWVACPSAI
                     INLSDIIAITKEK"
     gene            6133..6642
                     /locus_tag="VGT87_000014"
     CDS             6133..6642
                     /locus_tag="VGT87_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3812910.1"
                     /translation="MRTFSDTPKTFTFHYTFKDFDTAQVACHAILGYMTGTYEQPVID
                     ATYHNDDQGGHANQLVLKYAEDRKLSKVFKRICDSFKDYYNQPEDMTDEELDHQRVVS
                     LSKSTQGKVNNRDTLIAFISDHNQLAEHHSMNYKEMTPEDLGAILESISQAFNHLYDM
                     VVEGQLLVK"
     gene            6716..7204
                     /locus_tag="VGT87_000015"
     CDS             6716..7204
                     /locus_tag="VGT87_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3812911.1"
                     /translation="MLELSIENIIKPMKIQGKTRVTGSIGDQAIRIDLDGLVIHYNGQ
                     GLLLETIPGTYGGKRYFFVCPYCERRCRKLFKASHAFACGSCQKVHQATLNRSKTDCC
                     YYWQLAFKECLKVNPEARHIHGYYSRDDFPKRPKYMRLTKYLYHWRRFHYYMDKGDRY
                     WL"
     gene            7607..7969
                     /locus_tag="VGT87_000016"
     CDS             7607..7969
                     /locus_tag="VGT87_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEQ3812912.1"
                     /translation="MSAKEQLKELKPLFALITLFEEQRDKDIKLINAFHNPEAIRHIE
                     KGTAKQLLYLAKERDKRLAMIATLQDERQIAVIKARYVDDLSWDEILDKLGYSRNTVF
                     KLHREALEVLDEQEERYS"
     gene            7944..8327
                     /locus_tag="VGT87_000017"
     CDS             7944..8327
                     /locus_tag="VGT87_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HEQ3812913.1"
                     /translation="MSKKNAIRKLKEFHRWQRIANSLDLTYTELYQFDIEYHPTRRKH
                     LEISRECALEELDAIRYAINQLSKLDYRKILIECYLISEKKPQQDIIAELNRSQSWYY
                     ETKKRALLEFVEFYRDGVLKNNVRL"
     gene            8492..9145
                     /locus_tag="VGT87_000018"
     CDS             8492..9145
                     /locus_tag="VGT87_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3812914.1"
                     /translation="MWSSIFNFKENIVKKKLVMMLTLLISICAISVRANESGDLFTTI
                     GQSHLPGVTVAPFKTGDTTINVNLDPYGYVYGFVTSSNGNKIKKPENKTYEIPCSILE
                     KQDNRNCEGRKWTVSGNRADGGGNYTVQLEKPLAEGDKVTLSFADDGNLYFGQLVFED
                     GKTHNERLQKERDAEEQQYAEELFKRSIEEESKKTWHQRLSDSIQDQWWNFKDWLRG"
     gene            complement(9541..12096)
                     /gene="mutS"
                     /locus_tag="VGT87_000019"
     CDS             complement(9541..12096)
                     /gene="mutS"
                     /locus_tag="VGT87_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEQ3812915.1"
                     /translation="MAKTNISPGMQQYLDIKKDYPDAFLLFRMGDFYELFYEDAVKAA
                     QLLEIGLTSRNKNAENPIPMAGVPHHSAQQYIDVLIELGYKVAVAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSAKPDSANNFLVAVDFDGCRYGLAYMDVSTGEFCVTDLADFTS
                     VRSEIQNLKAKEVLLGFDLSEEEQTILVKQMNLLLSYEETVYEDKSLIDGQLTTVELT
                     AAGKLLQYVHKTQMRELSHLQALVHYEIKDYLQMSYATKSSLDLVENARTNKKHGSLY
                     WLLDETKTAMGMRLLRSWIDRPLVSKEAILERQEIIQVFLNAFIERTDLSNSLKGVYD
                     IERLSSRVSFGKANPKDLLQLGHTLAQVPYIKAILESFNSPCVDKLVNDIDSLPELEY
                     LIRTAIDPDAPATISEGSIIRTGFDERLDYYRKVMREGTGWIADIEAKERQESGINNL
                     KIDYNKKDGYYFHVTNSNLSLVPEHFFRKATLKNSERYGTAELAKIEGQMLEAREESS
                     SLEYDIFMCIRAQVETYINRLQKLAKTLATVDVLQSLAVVAETNHYIRPQFNDNHVIT
                     IQEGRHAVVEKVMGVQEYIPNSISFDQQTSIQLITGPNMSGKSTYMRQLALTVIMAQM
                     GSFVAADHVDLPLFDAIFTRIGAADDLISGQSTFMVEMMEANQAIKRASDNSLILFDE
                     LGRGTATYDGMALAQAIIEYIHDRVGAKTIFATHYHELTDLSTKLTSLVNVHVATLEK
                     DGDVTFLHKIAEGPADKSYGIHVAKIAGLPKSLLKRADEVLTRLETQSRSTEIISVPS
                     QVESSSAVRQGQLSLFGDEEKTHEIRQALEAIDVMNMTPLQAMTTLYELKKLL"
     gene            complement(12083..12435)
                     /locus_tag="VGT87_000020"
                     /pseudo
     CDS             complement(12083..12435)
                     /locus_tag="VGT87_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053122.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RicAFT regulatory complex protein RicA family
                     protein"
     gene            complement(12432..12869)
                     /gene="argR"
                     /locus_tag="VGT87_000021"
     CDS             complement(12432..12869)
                     /gene="argR"
                     /locus_tag="VGT87_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEQ3812916.1"
                     /translation="MNKMERQQQIKRIIQAEHIGTQEDIKNHLQKEGIVVTQATLSRD
                     LREIGLLKLRDEQGKLYYSLSEPVATPFSPEVRFYVLKVDRAGFMLVLHTNLGEADVL
                     ANLIDNDAIEDILGTIAGADTLLVICRDEEIAKRFEKDLAAGL"
     gene            13160..14851
                     /locus_tag="VGT87_000022"
     CDS             13160..14851
                     /locus_tag="VGT87_000022"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEQ3812917.1"
                     /translation="MDTKTLIASEIAKVVPELEQDAIFNLLETPKNSDMGDLAFPAFS
                     LAKVLRKAPQMIASELAEQIDESQFEKVVAVGPYINFFLDKTKISSQVLEQVITAGSD
                     YAQQDEGQGRNVAIDMSSPNIAKPFSIGHLRSTVIGDSLANIFAKMGYQPVKINHLGD
                     WGKQFGMLIVAYKKWGEEAAVQAHPIDELLKLYVRINAEAEIDPTVDEEAREWFRKLE
                     DGDKEATELWQWFRDESLLEFNRLYDQLHVTFDSYNGEAFYNDKMDEVLELLEAKNLL
                     VESKGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKRTYDFAKSVYVVGNE
                     QAAHFKQLKAVLKEMGYDWSDDMTHVAFGLVTKGGAKLSTRKGNVILLEPTVAEAINR
                     AASQIEAKNPNLADKEAVAHAVGVGAIKFYDLKTDRMNGYDFDLEAMVSFEGETGPYV
                     QYAHARIQSILRKADFTPSATTTYSLADAESWEIIKLIQDFPRIIKRTSDNFEPSIMA
                     KFAINLAQSFNKYYAHTRILDDNSERDNRLALCCATATVLKEALRLLGVDAPNEM"
     gene            14939..15247
                     /locus_tag="VGT87_000023"
     CDS             14939..15247
                     /locus_tag="VGT87_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEQ3812918.1"
                     /translation="MPSEKEILDALSKVYSEPVIQTDDYFRQAIFELASQLEKERMSS
                     LLATKIDSLINQYILTHQFDAPKSIFDLSRLVKTKACHYKGTAISAIMLGSFLSGGPK
                     "
     gene            complement(15274..16146)
                     /locus_tag="VGT87_000024"
     CDS             complement(15274..16146)
                     /locus_tag="VGT87_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEQ3812919.1"
                     /translation="MAHHDKLTKLLKLFLIALGVAIYTFGFVNFNMANALAEGGVAGI
                     TLILHAHFGINPAYSSLLFNLPLFILGAKIFGKRSLALTIYGTVLMSAFIWMWQKVPI
                     ELGLENDMMLVAVVAGLFSGIGSGIVFRYGATTGGTDIIGRIAEEKFGAKLGQTLLLV
                     DALVLTASLTYVDLKHMLYTLVASFVFSQMISVVQNGGYTIRGMIIITKHSEAAAQAI
                     LTEINRGVTYLKGQGAYSGNDYNIMYVTLNPTEVREVKRILAGLDPDAFISIIDVDEV
                     ISSDFKIRRRNYDK"
     gene            complement(16189..17067)
                     /locus_tag="VGT87_000025"
     CDS             complement(16189..17067)
                     /locus_tag="VGT87_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEQ3812920.1"
                     /translation="MSIDQRLQLKKFCFIILGAAIYAFAFVYFYMANRIAANGLAGLT
                     LVGKALFEIDPSLIGYLINLPLVLLGARCFGKRAMVYTVTGILSLYFFVWLFQRFPLV
                     VDLDHDNLVVSLMAGITGGLGGGLVFRNGGTIGGADIIAKLLEDKLGLQLNQALLGID
                     LFVMIVSLTYISLPQMMYALIASFMYGQIVRLVQQGGYSARGIFIVSEQAEKIARFIM
                     DELGRGVTYLTGEGAYSGRRKKVIYVALGRRDIRELKAFLTQVDPNAFITYFDVNEVN
                     SPEFLTIKSKYHKKRK"
     gene            complement(17087..18028)
                     /locus_tag="VGT87_000026"
     CDS             complement(17087..18028)
                     /locus_tag="VGT87_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEQ3812921.1"
                     /translation="MIKKTTYKKKVKYVISRGAKKVGLLHALRSISREKYAEKISASL
                     LYGILSSIAVNFFFQPGHVYSSGATGLAQVFSALSHRLLGYDIPIAFAFYLINIPLLI
                     LAWYKIGHQFTIFTFITVSMSSFFIQIMPQVTLTTDPLINAIFGGLVMGMGIGTGLKS
                     RISSGGTDIVSLTLRKRTGKDVGSLSLMVNGAILAFAGILFGWQYALYSMVSIFVSSR
                     VTDAIFTKQKKMQATIITSHPERVIHMIHKRLHRGVTSINDAEGTYKHEQKAVLITIL
                     TCEEYPEFKWLMLKTDPQAFVSVAENVRIIGRFVEDD"
     gene            complement(18021..19769)
                     /gene="aspS"
                     /locus_tag="VGT87_000027"
     CDS             complement(18021..19769)
                     /gene="aspS"
                     /locus_tag="VGT87_000027"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEQ3812922.1"
                     /translation="MKRSMYAGRVREEHIGTTITLKGWVSRRRDLGGLIFIDLRDREG
                     VMQLVINPEEVSSDVMATAERLRSEYVIEVEGFVEARQQANDKLATGMVELKVSALTI
                     LNTAKTTPFEIKDDVEVSDDTRLRYRYLDLRRPEMLENFKLRAKVTHSIRNYLDDLEF
                     IDVETPMLTKSTPEGARDYLVPSRVSQGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLSEQEIQDIVEGMIAKVMKETKEIDVTLPFPRMSY
                     DVAMNSYGSDKPDTRFEMLLQDLTVTVKGIDFKVFSEAPAVKAIVVKGNADRYSRKDI
                     DKLTEFAKQFGAKGLAWVKVTDGQLAGPAAKFLTAIETELSSQLKLAENDLVLFVADT
                     LEVANNTLGALRNRIAKDLDMIDQSQFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPT
                     PESAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKEMQERMFKALGFTADEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGKDNIREVIAFPKNNKASDPMTQAPSLV
                     SENQLEELSLQIESHD"
     gene            complement(20107..21387)
                     /locus_tag="VGT87_000028"
     CDS             complement(20107..21387)
                     /locus_tag="VGT87_000028"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEQ3812923.1"
                     /translation="MKLQKPKGTQDILPGDAAKWQYVESVARDTFSQYNYGEIRTPMF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PVKLYYIGSMFRYERPQAGRLREFHQIGVECFGAANPATDVETIAMAYHLFEKLGIKD
                     VTLHLNSLGSPESRAAYRQALIDYLTPMRDQLSKDSQRRLDENPLRVLDSKEKEDKLA
                     VEKAPSILDYLDEESQAHFEAVKDMLEALDIPYVIDTNMVRGLDYYNHTIFEFITSVE
                     GSDLTICAGGRYDSLVGYFGGPETPGFGFGLGLERLLMIIEKQGITLPIETEMDIYLA
                     VLGDGANSKALELVQAIRRQGFTAERDYLGRKIKAQFKSADTFKAKLVMTLGESEVEA
                     GKAVIKNNRSRQEVEVSFEDMMTNFENISEQLLS"
     gene            21607..21789
                     /gene="rpmF"
                     /locus_tag="VGT87_000029"
     CDS             21607..21789
                     /gene="rpmF"
                     /locus_tag="VGT87_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEQ3812924.1"
                     /translation="MAVPARHTSKAKKNKRRTHYKLTAPSVQFDETTGDYSRSHRVSL
                     KGYYKGRKIAKANEAK"
     gene            21805..21954
                     /gene="rpmG"
                     /locus_tag="VGT87_000030"
     CDS             21805..21954
                     /gene="rpmG"
                     /locus_tag="VGT87_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEQ3812925.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVT
                     FTEVK"
     gene            22247..22861
                     /locus_tag="VGT87_000031"
     CDS             22247..22861
                     /locus_tag="VGT87_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEQ3812926.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGLKVLLLGDSDGEAIAKEGLSK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFIAVVYLGLGMYILIENNSFDMLWAVLG"
     gene            22873..23211
                     /gene="cadX"
                     /locus_tag="VGT87_000032"
     CDS             22873..23211
                     /gene="cadX"
                     /locus_tag="VGT87_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cd(II)/Zn(II)-sensing metalloregulatory
                     transcriptional regulator CadX"
                     /protein_id="HEQ3812927.1"
                     /translation="MKKDSICQVGVINQQNVTTATNYLEKEKVQKSLRILSKFTDNKQ
                     INIIFYLLAVEELCVCDIACLLNLSMASASHHLRKLSNQNILDTRREGKIIYYFIKDE
                     EIRDFFNQLG"
     gene            23412..24185
                     /locus_tag="VGT87_000033"
     CDS             23412..24185
                     /locus_tag="VGT87_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3812928.1"
                     /translation="MLYLNTQTDSDYKEIIFGDIAKFVENMFYHYFSSILFRDLETVD
                     KRMYSFSDDDLISIQSSCLRLSKTFANFNIQRKNFLASDETEMDTFHKKDDIDITVGE
                     KSYNLARSFRTSTINELTVFNFEEMFDIIWLMLGDNLIKSFEVNVCGILFELNGNGIP
                     STFRQENIDPLINKWWYDNVSTEIIPNLIKKLKENPLFNIGFLVDDILERMYKENIPK
                     SYLTSVPLVISKKARSTLSIKSNGSQKIDKSKFAQILSL"
     gene            24251..25003
                     /locus_tag="VGT87_000034"
     CDS             24251..25003
                     /locus_tag="VGT87_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEQ3812929.1"
                     /translation="MAIPDLDKFSSSLQQGKIPVIKHFELNLDKGSHWAIAGNSGSGK
                     PYALTYFLSVLKPMSGLIIIDPKFDTPSQWARENKIAVIHPVENHSKSDFVSQVNEQL
                     NQCATLIQKRQAILYDNPNHQFTHLTIVIDEVLALSEGVNKNIKEAFFSLLSQIALLG
                     HATKIHLFLGSQRFDHNTIPISVREQLNVLLQIGNINQKTTQFLFPDLDPEGIVIPTG
                     HGTGIIQVVDNEHSYQVLPLLCPTYYTKRGIL"
     gene            25000..25602
                     /locus_tag="VGT87_000035"
     CDS             25000..25602
                     /locus_tag="VGT87_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3812930.1"
                     /translation="MTTKTYFMRSFNLILYLLLIAPICYVFGWLFNLVSVQLSTVTFV
                     NLDTVKNVTDTISSVCYGFALISLLLALLSGLKMIQRLNTDSLGNYCQSIYRTFLLRH
                     FLFQRERVQKVSNFEHQTVTTINPIHNGFNRAVRKCIVDIRKDAITVFLKVPRDQQGQ
                     KILKDTGVQLKEEIVIQHTDYYFSSPVRVRNQLWFIGQKR"
     gene            complement(26078..26359)
                     /locus_tag="VGT87_000036"
     CDS             complement(26078..26359)
                     /locus_tag="VGT87_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEQ3812931.1"
                     /translation="MLSKSLQVPLEQLIAEDIPKMTLIIKDEEIKQCDRNTHIMLFGT
                     LLIAISIYPIMSWLGWWGYGPVAILWLIIMRIALRIERFKKTYDVQTYR"
     gene            complement(26366..26410)
                     /locus_tag="VGT87_000037"
                     /pseudo
     CDS             complement(26366..26410)
                     /locus_tag="VGT87_000037"
                     /inference="COORDINATES: protein motif:HMM:NF013540.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            26813..27655
                     /locus_tag="VGT87_000038"
     CDS             26813..27655
                     /locus_tag="VGT87_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEQ3812932.1"
                     /translation="MVSFISRVFKGMIIALGFILPGVSGGVLAAILGIYERMISFLAH
                     MRDNFIENVLFFLPVGIGGILGIALFSFPVEFLLKHYQVIVLWGFAGAIVGTIPSLIK
                     ESTKQSQRDKVDWLWLVLTFVTSGLGLYFLNDLIGTLPANFLTFILAGALIALGVLVP
                     GLSPSNLLLILGLYGPMLIGFKSLDLLGTFLPIAIGGVLAILAFSKSMDYALQHHHSK
                     VYHFIIGIVLSSTLLILIPNSSSPESISYSHAGILTWLMTFVLFALGIWLGLWMSQLE
                     EKYK"
     gene            complement(27702..28343)
                     /locus_tag="VGT87_000039"
                     /pseudo
     CDS             complement(27702..28343)
                     /locus_tag="VGT87_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991395.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
     gene            28548..28874
                     /locus_tag="VGT87_000040"
     CDS             28548..28874
                     /locus_tag="VGT87_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEQ3812933.1"
                     /translation="MYFPVSATLIEFLILAIVEKNDSYGYDISQTIKLVANIKESTLY
                     PILKKLQKAGFLTTYSQEHQGRKRKYYAVTSSGRAQLIFLKKEWQSYKFALDGIIEGS
                     LRHDKD"
     gene            28861..29448
                     /locus_tag="VGT87_000041"
     CDS             28861..29448
                     /locus_tag="VGT87_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEQ3812934.1"
                     /translation="MTRTEYLAELDKYLRKLPREDYHEAMEYYIEYFDEAGPDKESQV
                     IDDLGNPKEAASEIISNVLGKHLATPEKTPKNRATIIGLTILSLFAAPIALPVLLALI
                     LFIIACLMLGLTAIFAAYIFGIAGILVAGVTLFESLTLIGSSIPAQAIGIGSALLCFG
                     GGLLIWIIATAILRFSGRAFVAFIKWISNKKGAQA"
     gene            29445..30518
                     /locus_tag="VGT87_000042"
     CDS             29445..30518
                     /locus_tag="VGT87_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEQ3812935.1"
                     /translation="MKNISRKCFMTSVVCIILGGILLGAGYATGGLQDIKHQTAPKKV
                     IKTFDQITALDIDSSASTITVETGPVQRPTVTYYTHPKFIDPLEIKIENETLHISEKP
                     RDVIITGGMELIGFGLNKSDRNHNYRTTTITLPKGTSLKELKGSSMPQATISDLTIQD
                     LEFSGGLTLLNTRIKKGKVFGTFDATDSLLANLELKADYSLSSLTNSSLENGTISLGH
                     GQLTTKNTNLKSVTIQSLSPGGVEAEETSLENVTFTVTSEQENEETNDNGAIFTAHAL
                     TLKGTNTISGGDVAVDITLTNPKTTAYKTKTEDGKISLGSQLTPAKIGKESTSDVISY
                     VAENKAATGNLTVNLNKGDITIK"
     gene            complement(30634..32907)
                     /locus_tag="VGT87_000043"
     CDS             complement(30634..32907)
                     /locus_tag="VGT87_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEQ3812936.1"
                     /translation="MLEELKTLIKNPKLMITMIGVALVPALYNLSFLGSMWDPYGRVN
                     DLPIAVVNHDKPAKRADKSLTIGDDMVDKMSKSKDLEYHFVSAKQAQEGLKEGDYYMV
                     ITLPEDLSQRAATLLNPEPQKLTIRYQTSKGHGMVAAKMGETAMAKLKESVSQNITKT
                     YTSAVFSSMTDLQSGLKEASAGSQALASGAKTAQAGSQTLSTNLAALTGASQQFQQST
                     GRLTSGLTTYTDGVNQVKNGLGTLSTDIPNYLNGVSRLSQGASQLNQGLSQLTQATTL
                     SDEKAKGIQSLIVGLPVLNQGIQQLNTELSTLQPPNLNADELGNSLGAIAQAAKQVIV
                     EETAAQNEELSALQATSVYQSLTAEQQGELAAALSQSDKSQTVSAAQTILSSVQTLST
                     SLQSLSQEDQSKQLEQLKEAVAQIANQSNQALPGASSALTELSTGLAKVNGSLNQQVL
                     PGSNQLTTGLAQLNRYNTAIGSGVIKLSEGTNALSSKSGELLDGSHQLSEGATKLADG
                     SSQLSQGGHQLTSGLTELSTGLSTLNGSLAKASQQLSLVSVTDKNAKAVAKPLVLNEK
                     DKDGVKTNGIGMAPYMIAVSLMVVALSTNVIFANSLSGRPVKDKWDWAKQKFVINGFI
                     STMGSIVLYLAIQLLGFEARYGMETLGFIMLSGWTFMALVTALVGWDDRYGSFASLVM
                     LLLQVGSSGGSYPIELSGAFFQKLHPFLPMTYVVSGLRQTISLSGHIGVEVKVLTGFL
                     LAFMVLALLIYRPKKTV"
     gene            33042..33575
                     /locus_tag="VGT87_000044"
     CDS             33042..33575
                     /locus_tag="VGT87_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEQ3812937.1"
                     /translation="MVNRKENTKQAILKAMVMLLKTESFDDITTVKLSKRAGISRSSF
                     YTHYKDKYEMIDYYQQTFFHKLEYIFEKKYQNKEQAFLEVFEFLQREQLLSSLLSANG
                     TKEIQAFIINKVRLLITTDLQDKFRTEELSQTEKEYQSIYLAHAFFGVCQSWIAKGKK
                     ESPQEMTQFVLKMLTST"
     gene            complement(33729..34340)
                     /gene="rpsD"
                     /locus_tag="VGT87_000045"
     CDS             complement(33729..34340)
                     /gene="rpsD"
                     /locus_tag="VGT87_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEQ3812938.1"
                     /translation="MSRYTGPSWKQSRRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFSYGLGEKQFRNLFVQATKIKEGTLGFNFMVLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVDPGQVISVREKSMKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(35072..35344)
                     /locus_tag="VGT87_000046"
     CDS             complement(35072..35344)
                     /locus_tag="VGT87_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEQ3812939.1"
                     /translation="MSDAFTDVAKMKKIKEDIRAHEGQLVELTLENGRKREKNKIGRL
                     IEVYSSLFIIEYSDSSDTPGAIDNSYVESYTYSDILTEKTLIRYLD"
     gene            complement(35361..36728)
                     /gene="dnaB"
                     /locus_tag="VGT87_000047"
     CDS             complement(35361..36728)
                     /gene="dnaB"
                     /locus_tag="VGT87_000047"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEQ3812940.1"
                     /translation="MRLPEVAELRVQPQDLLAEQSVLGSIFISPDKLIAVREFISPDD
                     FYKYAHKIIFRAMITLSDRNDAIDATTIRTILDDQDDLQSIGGLSYIVELVNSVPTSA
                     NAEYYAKIVAEKAMLRDIIARLTESVNLAYDEILKPEEVIAGVERALIELNEHSNRSG
                     FRKISDVLKVNYEALEARSKQTSNVTGLPTGFRDLDKITTGLHPDQLVILAARPAVGK
                     TAFVLNIAQNVGTKQKKTVAIFSLEMGAESLVDRMLAAEGMVDSHSLRTGQLTDQDWN
                     NVTIAQGALAEAPIYIDDTPGIKITEIRARSRKLSQEVDGGLGLIVIDYLQLITGTKP
                     ENRQQEVSDISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDA
                     DIVAFLYRDDYYRKEGDDAEEAVEDNTIEVILEKNRAGARGTVKLMFQKEYNKFSSIA
                     QFEER"
     gene            complement(36758..37210)
                     /gene="rplI"
                     /locus_tag="VGT87_000048"
     CDS             complement(36758..37210)
                     /gene="rplI"
                     /locus_tag="VGT87_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEQ3812941.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATSQSI
                     GELKGKQKAEEKAQAEILAEAQAVKAVLDEDKTRVQFQEKVGPDGRTFGSITAKKISE
                     ELQKQFGVKVDKRHIVLDHPIRAIGLIEVPVKLHKEVTAEIKLAITEA"
     gene            complement(37207..39183)
                     /locus_tag="VGT87_000049"
     CDS             complement(37207..39183)
                     /locus_tag="VGT87_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEQ3812942.1"
                     /translation="MKKFRFETIHLIMMGLILFGLLALCVSIMQSKILILLAIFLVLL
                     FVVALLWYQKEAYQLSDLAHIELLNEQTEDNLKTLLDNMPVGVVQFDQETNAVEWYNP
                     YAELIFTTEEGFIQNSLIQQIITEKRREDISQTFEVSGNKYTSYIDVSSGIFYFFDSF
                     VGNRQLADASMLRPVVGIISVDNYDDITDDLSDADTSKINSFVANFIDEFMESKRIFY
                     RRVNMDRYYFFTDFKTLNDLMDNKFSVLEEFRKEAQDAQRPLTLSIGISFGEENHSQI
                     GQVALENLNIALVRGGDQIVIRENADHTNPIYFGGGSVSTVKRSRTRTRAMMTAISDR
                     IKMVDNVFIVGHRKLDMDALGSAVGMQFFAGNIIENSFAVYNPDEMSPDIERAIERLQ
                     ADGKTRLISVSQAMGLVTPRSLLVMVDHSKISLTLSKEFYEQFQNVIVVDHHRRDDDF
                     PDNAILTFIESGASSAAELVTELIQFQNAKKRLNKIQASVLMAGIMLDTKNFSTRVTS
                     RTFDVASYLRSKGSDSVEIQNISATDFEEYKQINEIILQGERLGDSIIVAAGEKNHLY
                     SNVIASKAADTILSMAHVEASFVLVETASHKIAISARSRSKINVQRVMEKLGGGGHFN
                     LAACQLTDISLPQAKHLLLKTINMTMKETGEVES"
     gene            complement(39273..41171)
                     /gene="mnmG"
                     /locus_tag="VGT87_000050"
     CDS             complement(39273..41171)
                     /gene="mnmG"
                     /locus_tag="VGT87_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEQ3812943.1"
                     /translation="MTHEFTESYDVIVIGAGHAGVEASLATSRMGCKTLLATINLDML
                     AFMPCNPSIGGSAKGIVVREIDALGGEMGKNIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KSLYAREMKHTVEKQANLTLRQTMIDDILVEDGRVVGVLTATGQKFAAKAVVVTTGTA
                     LRGEIILGELKYSSGPNNSLASVTLADNLKKLGLEIGRFKTGTPPRVKASSINYDQTE
                     IQPGDDKPNHFSFMSKDADYLKDQIPCWLTYTNQTSHDIINQNLYRAPMFSGIVKGVG
                     PRYCPSIEDKIVRFAGKERHQLFLEPEGRDTEEVYVQGLSTSLPEDVQKDLIHSIKGL
                     EKAEMMRTGYAIEYDIVLPHQLRATLETKLISGLFTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKPELILKRSDAYIGVMIDDLVTKGTLEPYRLLTSRAEYRLILRHDNADMR
                     LTEIGRDIGLVDDERWKAFEIKKNQFDNELKRLNSIKLKPVKATNDRVQELGFKPLTD
                     AMTAKEFMRRPEIDYATAVSFVGYVAEDLDAKIIELLETEIKYEGYIRKALDQVAKMK
                     RMEEKRIPANIDWDAIDSIATEARQKFKKINPETIGQASRISGVNPADISILMIYLEG
                     NGKAHRKY"
     gene            complement(41295..41612)
                     /locus_tag="VGT87_000051"
     CDS             complement(41295..41612)
                     /locus_tag="VGT87_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEQ3812944.1"
                     /translation="MIVRNGKNFLTRDAADQYYTIGGTSLVGEKTHETVLRETLEEVG
                     IRAKVNQLAFMVENHFDIDDVFWHNIEFHYLVSPLEDPKLEMIENASDRFVNGFQSIN
                     WIK"
     gene            complement(42387..43508)
                     /gene="mnmA"
                     /locus_tag="VGT87_000052"
     CDS             complement(42387..43508)
                     /gene="mnmA"
                     /locus_tag="VGT87_000052"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEQ3812945.1"
                     /translation="MTDNSKIRVVVGMSGGVDSSVTALLLKEQGYDVIGVFMKNWDDT
                     DEFGVCTATEDYKDVAAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYAQVKRDENGTVHMLRGADNGKDQTYFLSQLS
                     QEQLQKTLFPLGHLQKSEVREIAERAGLATAKKKDSTGICFIGEKNFKQFLSQYLPAQ
                     KGRMMTIDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNQPWFVVGKDLSQNILYVGQ
                     GFYHEALMSNSLDASVIHFTREMPEEFTFECTAKFRYRQPDSHVTVHVRGDKAEVVFA
                     EPQRAITPGQAVVFYDGKECLGGGMIDMAYKNGQPCQYI"
     gene            43806..44477
                     /gene="sdaAB"
                     /locus_tag="VGT87_000053"
     CDS             43806..44477
                     /gene="sdaAB"
                     /locus_tag="VGT87_000053"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEQ3812946.1"
                     /translation="MNTQKFQSVFDIIGPVMIGPSSSHTAGAVRIGKVVHSIFGDIPD
                     EVTFHLYNSFAKTYRGHGTDKALVAGIMGMDTDNPDIKNSLEIAHQKGIKIYWDILKD
                     SNAPHPNTVKISVKKADKTLSVTGVSIGGGNIQVTELNGFSVSLSMNTPTIVTVHKDI
                     PGMIAKVTDILSSNNINIATMNVTRESAGEKATMIIEVDSRECQEAANQIAKIPHIYN
                     VNFFD"
     gene            44489..45361
                     /gene="sdaAA"
                     /locus_tag="VGT87_000054"
     CDS             44489..45361
                     /gene="sdaAA"
                     /locus_tag="VGT87_000054"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEQ3812947.1"
                     /translation="MFYTIEELVKQADQQFNGNIAELMIATEVEMSGRNREDIIKIMS
                     RNLQVMKAAVTEGLTSTKSISGLTGGDAVKMDNYIKKGNSLSDTTILNAVRNAIAVNE
                     LNAKMGLVCATPTAGSAGCLPAVLATAIEKLDLSEKKQLEFLFTAGAFGLVIGNNASI
                     SGAEGGCQAEVGSAAAMSAAALVKAAGGTSHQASQAIAFVIKNLLGLVCDPVAGLVEV
                     PCVKRNALGASFALVAADMALADIESQIPVDEVIDAMYQVGSAMPTAFRETAEGGLAA
                     TPTGRRYSVEIFGE"
     gene            complement(45775..46389)
                     /locus_tag="VGT87_000055"
     CDS             complement(45775..46389)
                     /locus_tag="VGT87_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEQ3812948.1"
                     /translation="MFKKENLKQRYFNFGLVALALTILAIIFAFSSKNADTKSYAKKS
                     ESKMVTIDKVPKNNHAITKEESKEKAKSIASEPIPTVENSVAPTVTEEAPVVQQEVTQ
                     TVQQVSSVAYNPNNVVLSNGNTAGIVGSQAAAQMAAATGVPQSTWEHIIARESNGNPN
                     ATNASGASGLFQTMPGWGSTATVEDQVNAALKAYSAQGLSAWGY"
     gene            complement(46761..47561)
                     /locus_tag="VGT87_000056"
     CDS             complement(46761..47561)
                     /locus_tag="VGT87_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEQ3812949.1"
                     /translation="MDKLILGRYIPGDSLIHRLDPRSKLLAMIIYIVIIFWANNVVTN
                     LLMLTFTLAVVFLSKIKLSFFLNGVKPMIGIILFTTLFQMFFSQGGKVIFSWWFISIT
                     DLGLSQAILIFMRFVLIIFFSTLLTLTTTPLSLSDAVESLLKPLTRFKVPAHEIGLML
                     SLSLRFVPTLMDDTTRIMNAQRARGVDFGEGNLIQKVKSIIPILIPLFASSFKRADAL
                     AIAMEARGYQGGEGRTKYRQLDWQLKDSLAIGIVSLLGLLLFFLKTSL"
     gene            complement(47554..>48090)
                     /locus_tag="VGT87_000057"
     CDS             complement(47554..>48090)
                     /locus_tag="VGT87_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEQ3812950.1"
                     /translation="VLKDVAFGPQNFGVSPEEAEALAREKLALVGISENLFEKNPFEL
                     SGGQMRRVAIAGILAMQPKVLVLDEPTAGLDPKGRKELMTIFKKLHQSGMTIVLVTHL
                     MDDVANYADFVYVLDKGKIILSGKPKTIFQQVSLLEKKQLGVPKVTKLAQRLVDRGIP
                     ISSLPITLEELREVLKHG"
BASE COUNT        15544 a         9524 c         8416 g        14606 t
ORIGIN      
        1 gtactacgaa aaattttcgt agtggtcaaa aagtttggtt tttaactgag catggacttt
       61 atgaagtgct tatgcgttca aacaaaccaa aagccaaaga gtttagaaaa gcagtcaaaa
      121 acattctaaa agaaatccgc ttgaatggct attacatgca aggcgaattg gtgcaagaac
      181 tagctcaatc aagcacccaa aaactaccag gtataagtga cctaacttat atacgaaata
      241 agctagctga tttagttgat atggataatc tagctgatat ttcaaatggg attgaccgag
      301 ttcagcaact agtgaagctg atcagcttgt aggagcaatc tatgaacaaa acagaaacat
      361 ggaacggata tactatccga tttgtagagc accaaggcga atggtgggcg gttgccaagg
      421 atattacaaa tgctttggga ttaaaacaac cgtcaagagc tatatcgact ctaaaggggg
      481 ttactaaaag taagaccctt acaaaaggtg gggagcaaga gctttctatt atcaatgaaa
      541 aagatattta cagattagta ttcaaaagtc gaaaacctga ggcggaagct ttccaagaat
      601 gggtatttga aaccattaaa cagttaagac aaagcacagg cctagagggc ttccaagtat
      661 ttagaatgtt tgataaagaa catcagaagc agtcaatgaa taggcttgtc aatggcttac
      721 aaaatgcaac taagaaagac cttatcaaag ccaacacgat tgccaataaa gcggtatcta
      781 ataagtatgg gttaccaaag atgattagta aaaacgagat gacagaaaac atgttgcgag
      841 accgtgagcc tatccttgat gaaacggttg aactaatcaa ggttaaagaa aaatacggct
      901 taaattttag cgtatctgaa gcaatcttta accaaaacac aataaaaaaa gcgcagtgat
      961 gcgcgtggat aaaggagaaa tgattatgaa agcaacaact tacaaagagt tgaagaaatg
     1021 gattgatgaa ggtgttgatt tagctgagct agcacagggt tacgctgaca aagtaccaaa
     1081 tgcagatcgc gaacagtttg aagcaatcac acaggaaatt ttcaacgtat tggaaggcgt
     1141 atcgctcatg cttgatgaca aagtgctaat ctataatcgc aaagcagagc aaaagcgttt
     1201 gaatgacatt gaacaaggca attattaatc aataatcaag agcaacaaaa aaaggctttg
     1261 acagcgacca aacttccaag cctttaatta gtataactaa actcaattaa taaagcaggc
     1321 aagctattat taaaggggtt ttatgtctta aattatagca tattgaagct attttgacca
     1381 tacgtaggga gctacccctt aaaactagca tgaatctagt ataagaaaca agcaatcaat
     1441 aaaaacacta cacagaaaat aataattaag cgagaaaaaa cataatgaaa tatagagtag
     1501 aaacaaatcc tttttcaaaa gatagataca ctcctgaaca gctagaaatg ttcaaaaatc
     1561 gccaactcag caaaaataaa gcagaagcct atttcactcg actatataac caacatatcg
     1621 cttgggtaat tattgctaac gttatgacag agtacgtcat taaattcaga aaaagtgcca
     1681 ccagctttga agaagcatgg gacgctttag actatcaacg aaccacagag attgtcttta
     1741 gagccgttaa cggtttacct tgttcagaga aagactcagg ggaactagaa acttatttaa
     1801 gtgaggtatc ggcatgatgc aagaacttaa cctcacacca acacagacgc ttattctatt
     1861 ctttgtttta agtctcttag ggcttcttct tagcctttct aagtcattaa tagacattga
     1921 tttaccagaa gatacccaag tccctaaacc acctaagaac gcaaactatg gggcttatat
     1981 tcaatcacag aaccattatt acaattaggg aggaactgca tgacaacgga agaatatttt
     2041 aaaaaattgc tagaagatag cgaaaaacag cctacaaact ggttgagtga tagggtttta
     2101 gacaaaaata tcaaaatgct agatgaagat attgaagcag agtgggacga ttttccgtta
     2161 ttcacgaaaa aaatatttat caatactaca caacgtaact ataacaaagc tgatgccgtt
     2221 gttaaatttt tggaatcaat accaatagag atggaatata aaaaagggct aaatatagtt
     2281 atacaagcat tgaacgagtt aaaagatagt cttaaaaatc aagtgcttgg tgttattgat
     2341 aaccaaatcc tgtttccaaa tgataaggag gacacgaaat gacactacca gagaattata
     2401 gacgtgtcct taatctgatt aaggttgggg cagagattag cttaatactg aaacttgaag
     2461 aacgctccgt ccaaagtatc atcagtagct taatcacgcg ctataacgtc cctattatcg
     2521 gcattaggca cggattcaat cgtggttact ttattccagc taacaaagaa gaattgctag
     2581 acggtgctaa agccttttac aaccaagtac aaaaggaaca agaacgccta agtgttttat
     2641 tgaatgccga tttaaccagt tataaggaat tactcaaagg aggttaggca tgaacgtatt
     2701 tagtcaagat tatgaatcca aactcttaga acaaaacctg accgctttta atcgcttttt
     2761 ggaagcctac cagaaaccta aaccaagagt tttagggttg atcacggccg aacaagtcaa
     2821 agaggaatta aatatcaaag gtaaaaccct aaaacggtgg gaaaatgctg ggttaagacg
     2881 ataccaacca ccactagaag acaccaggaa acattattac aaggtcagtg atattcttat
     2941 ctttttgggg gtaagtgtgt agatggctat ttatgaagca agaggcttta gctcttattt
     3001 gtacccctac aaaggacctt tagaaccatt tgactatatt gctcaattta gacctttgaa
     3061 accgcctgag gatattgata ttgaagaata caagcgaaca caagccccct actgcctaag
     3121 tggcaaggtc acagccgaga aaaacggtag ctataagcgc aataatgcta gtttagttta
     3181 tcgcgatttg atttttcttg actatgacga gatagaaaca ggcgtcaacc tacctaaaat
     3241 cgtttctcag acgctttggg agtatagtta tattatttat ccaacgatta aacacacccc
     3301 cgagaagccc cgttatcgcc ttgtcatgaa gcctagtgac gtgatgactg aagcaactta
     3361 taaacaagtg gtcaaggaga tagccgataa gattggacta ccgtttgatt tagctagcct
     3421 tacctggtca caattacaag gcttacctat tacaacaggc gacccagagg actatcagcg
     3481 ctatgtgaac cgtggtcttg gttatcctgt tcctaaaaat ggtaacacgc caaacagaca
     3541 agttgttact acttacacgc cacgccctag aagtcagcgt tctattacca tgagggtcat
     3601 agataccctg tttaatggtt ttggagacga aggcgggcgc aacgtggcct taactaaatt
     3661 tgttggcttg ctatttaata aatgggtgga ttgtgattta gagacggctt atgagctggt
     3721 acaaatagct aacagcgtga caactaagcc actacccatt gatgagatag atacaacctt
     3781 tagaagcata cttgataaag aattaagaaa gagaggaatc aagccataga caaagaagaa
     3841 ttgaaagact atcaaaataa actatcgcaa gccactcaac ctgcttttgc ccctgctttc
     3901 aggacacgaa aaggtagagg agacaaagaa tatgtcatta gtagccccta caatgtcggt
     3961 aaggtttttg aattttacga aaatatctat acaggtatta aatacaacga atttgaaaaa
     4021 actattgaaa tcactaaagc agttccttgg tctaaagaaa aagggctatg gacgaacgag
     4081 cagaccagcc tttgtattgc attcattgat gaaaaatatc ggtttacccc tcgtaaagaa
     4141 catatagagg tagctattac cgctttagct aaaaagaaca cttatcaccc cattaaacag
     4201 cgtattgaaa gtcaaaaatg ggatggtaaa gctagaggag aacgctactt tattgatcta
     4261 ttaggctgtg ctgataattc ctataataga gaaattgcca aagtatggct aacaggtctc
     4321 atggctagaa tttatctccg taaagtaaag tttgaagtcg ttcctattct cattgataaa
     4381 agacaaggaa ctgggaaaag caccgttact aagcgactac tccctagcta ccacactgat
     4441 tcagaaatca agtttggtaa aaatgatagt gattatcaga agatacaagc caatgccatt
     4501 attgagctag gggagctaaa aggcatgtca aaggcagaaa ttgaaacagt taaaagcttc
     4561 atttcctcag atagtgatac ttatcgtgaa ccttatgaac gtaaagccac tcctcatcca
     4621 aggcactgtg tctttatcga acagctaata aaaaatcttt tcttaaggat agtggaacag
     4681 aaagacgctt tttccctatt gaatgtggta tcaatgacgt gaaaaaacat cctatggagg
     4741 tggaagaaga ttatttctta caggtactcg ctgaagccaa agtatggttt aacaattatg
     4801 aaccactaac gccatctaaa gagttaatga atcagttagc agacattcaa gaagattata
     4861 aggttgaaga cgtcgacaaa gaaattatcg atcaattact gaatgagttt caaattgttg
     4921 aaggttggga tagcttatca cagtatgaac aacggcaata catcctcaaa cagctaggag
     4981 agccgttaga taatgctcaa agctatagtg actacccttc cgcacagaca gattgcttac
     5041 tccaagtgac aagtcctaac catatcgctt atctaggatt taaccaaaaa ccaacgcaag
     5101 gtggtaaggc ccttatttct caaaaaatac gtgattactt agataatgat gacggctgga
     5161 aaaaaggaga gaatcccgca agaaaaagac tatttaaagg tggaactccc gtaccttact
     5221 atgaacgagt ttaaaaacta cactaatgct acataaaaac tacacgtaat gatacaagct
     5281 aaaaccttgg tattataggc tttatactct attgtagtat tagtagtatt taatattata
     5341 gataataata tttataacta atagtgcgcg tgcaaaaaaa gaaagtcttt tgctcaaatt
     5401 aatgctacta aaagcacaaa gtgcccaacc cccttgatac cactggcttt atccctgtgc
     5461 tttttgctgt gctatttgat ttttaaaaaa acaaaaagca cacattttag agagaaaaga
     5521 gggacatatg aatcctaact atacaaatca tggcgtaaag aaaatgcgtt acaattatga
     5581 tgatttgcca aacgaccagc tataccgatt ggaacttcct aatggtcaat gcggttatga
     5641 ctttatcacc aaaattaatt tcaacacaaa gtctagaccg ggtgattgtg attttaccag
     5701 accagaacta acacgacgtg gttaccaatg ggtctttgac tgtggctttg cgatacctgt
     5761 tgagaaagga gctaaaacat gaaaatcaaa ctattttatc aaaaacacaa tgaatcacta
     5821 gatgattttg aacatcgggt caatctcttt accctatcgg tatctgtgat agacattaaa
     5881 ttccaagaag ccacttatgg caattatgaa gacatggatt caagaacagg actactggtc
     5941 ttgtacaggt aactgatatg aaactaaaat tacacacacg aggtggcaat accataacca
     6001 tacaaggaga ccgcaccctc tatgatgagt tgaccaaata tcttttatct ggccaacaac
     6061 caaactgggt ggcatgtcct tctgccatca tcaatttatc agacattata gcaatcacaa
     6121 aggagaaata gcatgagaac attttcagac acaccaaaga cattcacatt ccactacacg
     6181 tttaaagact ttgataccgc acaagtagcc tgccatgcta ttttaggcta tatgactggg
     6241 acctatgagc aaccagtgat tgacgcaact tatcacaatg atgaccaagg tggtcatgct
     6301 aatcagttag tcttaaaata tgctgaagac agaaagttaa gcaaggtctt caagcgtatc
     6361 tgtgatagtt tcaaggacta ttacaaccaa cctgaggata tgacggatga agaacttgat
     6421 catcagcgcg tggtttcttt atctaaaagt actcaaggga aagttaataa tcgagacaca
     6481 cttatagcct tcatctccga tcacaaccaa ctggctgaac accactctat gaattataaa
     6541 gagatgacgc cagaagactt aggggccatc cttgagtcta tcagtcaagc ctttaaccat
     6601 ttgtatgata tggttgttga aggtcagtta ctcgttaaat agacattcag agggttttcc
     6661 ctctttttgt cgttttatca atagttttgg gttgtttgag ttttaaggag aaagaatgtt
     6721 agaactatct attgaaaata tcattaaacc aatgaagata caggggaaga caagagttac
     6781 aggatcaata ggtgaccaag ctatccgcat agacctagat gggctagtga ttcactacaa
     6841 tggtcaaggc ctattgcttg aaacgattcc aggaacttat ggtggcaaac gttacttttt
     6901 cgtgtgtcct tactgtgaga gacgttgtcg gaaattattt aaggcttctc atgcctttgc
     6961 ttgtggttct tgtcagaagg ttcatcaagc cacgctcaat cgaagcaaga cagactgttg
     7021 ctactactgg caattagcct ttaaagagtg tttgaaagta aatccagaag caagacacat
     7081 tcatggatat tatagtcgtg atgactttcc taaacgtcca aagtacatga gattaaccaa
     7141 atacctttat cactggagaa gatttcatta ctatatggat aagggtgaca ggtattggct
     7201 ataatattcg gaaactaccc ccttcatttt taaacggggc tatattgttc ggaaacttaa
     7261 gaacgcgccc ttttccgtgc aaaaaattcc ctttttgaaa ttttcaccga agattaaaaa
     7321 cttgatatga aaggatttga catctcattt tagcccataa ccctgccaca aagaataacc
     7381 atctaagact aaaataaaac attgttttag cgcttctagc tactaagttg atgatttata
     7441 ctataaactc taaaaagtgc ttagaaacga ttttagaagc caaaagcgaa gtactacaaa
     7501 aaatatctag tttacaaaat gaacaaaaca aaaagacgtc cacacggaac accccttggt
     7561 taaatttaag cttaaataaa ttataccata accaggagaa aagaccatga gcgctaaaga
     7621 acaacttaaa gaattgaaac cacttttcgc tttaataacc ttatttgagg aacaacgaga
     7681 taaagacatc aagctgataa atgcttttca taatcctgaa gcaataagac atatcgaaaa
     7741 aggtacagct aaacaactct tatatttagc taaagaacgt gataagagac tagccatgat
     7801 cgccacactc caagatgaga gacagatagc tgttattaag gctagatacg tggatgactt
     7861 atcatgggac gagatactcg ataaactagg ttactcaaga aacactgtgt tcaaactaca
     7921 tagagaagct ttagaggtgt tagatgagca agaagaacgc tattcgtaag ctgaaagaat
     7981 tccatagatg gcaacgtatc gctaatagcc ttgatttaac ttataccgag ctttaccagt
     8041 ttgatataga gtaccatccc acgcgcagaa aacaccttga aataagccga gaatgcgccc
     8101 tagaggaact agacgctatt aggtatgcca ttaatcaact gtctaaacta gattacagaa
     8161 agatactgat tgagtgttac ttgatcagtg agaaaaaacc tcaacaagac atcatagcag
     8221 aacttaacag aagtcaaagt tggtactatg agactaagaa acgagctttg cttgagtttg
     8281 tggaatttta cagggatgga gtgctaaaaa ataatgttcg tttatgacaa tggtttaaga
     8341 ttaatacgtc tctaatacag ttaacacatc catctagaaa ataacgatta gaagaaacga
     8401 acttggtatc gtttgcaaag ctgacttggt gggtcaaatt agacataaaa tatcaaaaag
     8461 tagaatggat tttttgactg attaactggt attgtggagt tcgatattta attttaagga
     8521 gaatatagtg aaaaagaaat tagttatgat gttaactttg ttaatatcaa tatgtgctat
     8581 aagtgtcaga gctaatgaaa gtggcgattt gtttacaact atagggcaat cgcacttacc
     8641 tggagtaacc gtagcaccat ttaaaacagg agatacaacc attaatgtta atctagatcc
     8701 ctatgggtat gtttatggtt ttgtaacttc aagtaatggt aataaaatta aaaaacccga
     8761 aaacaaaact tatgaaattc cttgctctat tttggagaaa caagataatc gaaactgcga
     8821 aggaaggaaa tggacagtaa gtggcaatcg tgctgatgga ggaggcaact acacagttca
     8881 attggaaaaa ccacttgctg aaggagacaa agttacctta tcttttgcgg atgacgggaa
     8941 tctatacttt gggcaacttg tatttgagga tggaaaaaca cataatgaac gtcttcaaaa
     9001 agagagagat gccgaagagc aacaatatgc ggaggagtta ttcaagcgtt ctatcgaaga
     9061 agaatccaaa aaaacttggc atcaacgctt gagcgatagc atccaagacc aatggtggaa
     9121 ctttaaagac tggttgagag ggtagttttt tcctcaatga cactaatcgt gctataataa
     9181 gccatagggc aaactaaaag cgtagtatct aaaccacgct agttcttgcc tgctgaactc
     9241 attgtgtaaa ggacctgcaa aggtcttttt ttgttcacct ttttgtggac ttttgaggga
     9301 agtctaagga aacttaaaac ctatattttt tcaaaaacac agtcatatca atgcttaagg
     9361 actgtcaata ccctgtaaag actgccaatc caagtccaat aatgtttgtc ataatttcct
     9421 cgcattctta tggactatta taacacactt aataaatagc cgaaggcctc ggaccgtttt
     9481 tgacagaaca aaaagatgct ttctatattg aaggcatctt tttcattttc agctgaaaga
     9541 ctataacaac tttttcaatt cgtaaagggt tgtcattgct tgaagcgggg tcatgttcat
     9601 gacatcaata gcttccagtg cttgcctaat ctcatgagtt ttctcttcat caccaaaaag
     9661 ggataattgc ccctgtctaa cagcgctgct tgactcaact tgtgaaggga ctgatattat
     9721 ctcagtagat cgtgactgtg tttctaaacg ggtcagaact tcgtctgctc tctttaatag
     9781 ggattttggc agtcctgcta tttttgccac atgaataccg taagatttat ccgccggtcc
     9841 ctcagcaatc ttatgaagga aggtaacatc gccatctttt tcaagcgttg ctacatgaac
     9901 attgactaga cttgtcaact tagttgacaa gtctgtcaat tcatgataat gcgttgcaaa
     9961 tatggtctta gcaccaactc tatcatggat atattcaata attgcctggg ctaaagccat
    10021 accatcataa gttgccgtac ctcgtcccag ttcatcaaat agaataagag agttgtcact
    10081 tgcgcgtttg attgcttggt ttgcttccat catctccacc ataaaggttg attgcccaga
    10141 aatcaaatca tcagcagccc caatacgcgt aaaaatcgca tcaaataaag gtaaatcaac
    10201 atggtcagca gccacaaatg aacccatctg ggccatgata accgttaagg ccagctgtct
    10261 catataagtc gacttaccac tcatatttgg acctgtaatc agctgaatac tggtctgttg
    10321 gtcaaaagag atactattgg gaatgtattc ctgcactccc ataacctttt caacaacggc
    10381 gtgacgacct tcttgaattg taatcacatg attatcattg aactgcggcc gaatataatg
    10441 attggtttca gcaactactg ctaaactttg caaaacatcc accgttgcca aagttttagc
    10501 cagtttctgt aaacgattaa tataggtttc aacttgagct cgaatacaca taaaaatatc
    10561 gtattctaaa ctagatgact cttccctagc ctctaacatc tgaccttcaa tcttagccaa
    10621 ttctgctgtt ccataacgct cagaattttt taaagttgcc tttctgaaaa aatgctcggg
    10681 aactaagcta agatttgaat tcgtaacgtg aaaataataa ccatcttttt tattgtaatc
    10741 aatttttagg ttattaatgc cactttcttg acgctctttg gcctcaatat ccgcaatcca
    10801 gcctgttcct tctcgcatca ctttacgata atagtccaag cgctcatcaa aaccagtacg
    10861 aataatactg ccttcactaa tagttgctgg tgcatctgga tcaatggctg ttctaatcaa
    10921 gtattccaac tcaggcaaac tgtcaatatc attgacaagt ttgtcaacac aaggactgtt
    10981 aaaagactct aagatagctt tgatataagg cacttgggct aaggtatgcc ccaattgaag
    11041 taaatctttc ggatttgcct tgccaaaaga cacgcgacta gataagcgtt cgatgtcgta
    11101 aacacctttt aaactattgc ttaaatcggt tcgctcaata aaagcattca gaaaaacttg
    11161 aataatttct tgacgctcta aaatagcttc tttagaaacc aaaggtcgat caatccatga
    11221 gcgcaaaagc ctcatcccca tagctgtctt ggtttcatct aaaagccaat acagacttcc
    11281 atgtttttta ttagtcctag cattttctac caaatctaaa cttgacttag tggcatacga
    11341 catctgcaaa taatccttga tctcatagtg aaccaaggct tgcaagtggc tgagttctcg
    11401 catttgtgtt ttgtgaacgt attgcaagag ttttcctgcc gctgtcagtt ctaccgttgt
    11461 caattggccg tcaattaaag atttatcttc atagaccgtt tcttcataag aaagcagcaa
    11521 attcatctgc ttgaccaaaa tcgtctgttc ttcttcagat aaatcaaaac ctagtaagac
    11581 ttcttttgcc ttgaggtttt ggatttcgct acgaacactc gtaaagtccg ccaaatctgt
    11641 cacgcaaaat tcacctgtgg atacatccat ataagccaat ccataacggc aaccatcaaa
    11701 gtcaacagct accaaaaagt tattggcgct atctggctta gctgaatcca caaccgttcc
    11761 aggagttatg acttgaacga cctcacgctt caccacccca acagcttgct ttgggtcttc
    11821 catttgttct gcgacagcaa ccttgtaacc caactcaatt aacacatcaa tgtattgttg
    11881 ggcagaatga tgtggcacgc ctgccatagg aattggattt tccgcattct tgttgcgact
    11941 ggtcaaacca atttctaaga gttgtgctgc tttgacagcg tcctcgtaaa ataattcata
    12001 aaagtcaccc atcctaaaaa gcaaaaaagc atctggataa tcttttttga tgtccagata
    12061 ctgttgcatt ccaggagaaa tgttagtttt tgccatttgt taactccttg tttaactgat
    12121 cttctatacg tttggtgata tactgaatca catcacttgc atcttgcatc ttttctcgat
    12181 aatcatcaat taatggctga tgactgagcc tatcttctag ttcttttgac ctgcttgcag
    12241 atactgtcgc agccttttct ttatctattt tttttgaaaa gcagggcatc ttgttgatag
    12301 cgtttcatct gataagctta ctttttaaaa tcatggagct tagacagcta ttgcttcact
    12361 tctttataag caacgatact agaattcatg gatagcaaga atcaattgtg tcaaagactc
    12421 ttaatagcta gtcataggcc tgcagccaaa tctttttcaa agcgtttagc aatctcttcg
    12481 tctctgcaaa tcacaagcaa ggtatccgca ccagcaatgg ttcctagaat atcttcaatg
    12541 gcatcattgt caatcaaatt agctagaaca tcagcctctc ctaaattggt atggagaacc
    12601 agcataaaac ctgctcgatc aactttcaaa acataaaaac gcacctcagg actaaatggt
    12661 gttgcaacgg gttcagacaa actataatag agtttgcctt gttcatctct caatttcaaa
    12721 aggccaattt ctcttaagtc ccgagataga gttgcttggg tgaccacaat gccttctttt
    12781 tgaaggtgat ttttaatatc ttcttgtgtg ccaatatgct ctgcttgaat gattcgctta
    12841 atctgctgtt gacgctccat tttattcatg atatatcacc ccaattgtat aattatcctc
    12901 tcaaattata acaaaaaaag tgtattattt catcttcgcc cagctatttt cagcaatttt
    12961 ttatcaaatc caactagaat ttcacttgaa aactgttgaa aagaaatctc atttctcttt
    13021 ttataacatt ttcagaaaaa taacaacctc aagcaattca tttccttgaa agtttcagta
    13081 taatttatca atcctctcct cgtttatttt aaagaaatat gttagaatag ggtgacaaac
    13141 aatttttagg agacgccgta tggatactaa aactctaatc gcaagtgaaa tagctaaagt
    13201 tgtccctgag ttagaacaag atgctatttt taacttgctt gaaaccccaa aaaattctga
    13261 catgggagac cttgcctttc cagcctttag tttagcaaaa gtccttcgca aagcccctca
    13321 aatgattgct agcgagttag ctgaacagat tgatgagagc caatttgaaa aagtcgtagc
    13381 tgtcggccct tacatcaact ttttccttga taaaactaag atttcctcac aggtccttga
    13441 acaagtgatt acagcaggtt cagactatgc tcagcaagac gaaggtcaag ggcgaaatgt
    13501 tgctattgac atgtcaagcc caaatattgc taaaccattt tcaatcggtc acctccgctc
    13561 aactgttatt ggtgatagcc ttgctaatat ctttgctaaa atgggttatc aacctgtaaa
    13621 aatcaatcac cttggtgact ggggtaaaca atttggtatg ctcattgttg cctacaaaaa
    13681 atggggagag gaagctgctg ttcaggcaca tcctattgat gaactcttaa aactttacgt
    13741 tcgtatcaat gctgaagctg aaattgatcc aaccgtcgat gaagaagctc gtgaatggtt
    13801 ccgtaaattg gaagatggcg acaaagaagc tactgaatta tggcaatggt tccgtgatga
    13861 aagtttactt gagttcaacc gactttatga ccaattacat gtcaccttcg atagttacaa
    13921 cggtgaggcc ttctataatg acaaaatgga tgaggttctt gagctattag aagcgaaaaa
    13981 ccttcttgta gaatccaaag gtgcccaagt tgttaacctt gaaaaatacg gcattgaaca
    14041 ccctgccttg atcaaaaaat cagatggcgc tactctttac attacccgtg acctagctgc
    14101 cgcactttac cgtaaacgca cttacgactt tgctaaatcc gtttatgttg ttggtaatga
    14161 gcaagctgct cacttcaaac agttaaaagc agtgctaaaa gaaatgggtt acgattggtc
    14221 agatgacatg acacacgttg cttttggact tgtcacaaaa ggcggtgcta aattatcaac
    14281 acgtaaaggt aatgttattc ttcttgaacc tacagttgct gaagccatta accgtgcagc
    14341 ttctcaaatt gaagcgaaaa atccaaacct tgctgacaaa gaagccgttg cccatgccgt
    14401 tggtgttggt gccatcaagt tttacgatct caaaacagat cgtatgaatg ggtatgactt
    14461 tgaccttgaa gccatggttt cctttgaagg agaaactgga ccttacgttc aatatgctca
    14521 tgctcgtatc caatctattc tacgtaaagc tgactttacg ccatctgcaa ccaccactta
    14581 cagtttagct gatgctgaaa gctgggaaat tatcaaactt atccaagatt tcccacgcat
    14641 tatcaagcga acatcagata actttgagcc ttctattatg gctaaatttg ctattaactt
    14701 agcccaaagc tttaacaaat attatgctca cactcgcatt cttgacgaca actcagaacg
    14761 tgataaccgc ttagctctct gctgtgcaac tgctactgtt cttaaagaag cgcttcgctt
    14821 actcggtgtt gacgcaccaa atgaaatgta agtaataaca ttactaaggc tgtcacattt
    14881 atgacagtct tagtttttta tattataata atagcacaat atgttacagg agaatgccat
    14941 gccatcagaa aaagaaatat tagatgctct tagtaaagtt tatagcgaac cggtaataca
    15001 aaccgatgat tattttcgtc aagcgatctt cgagctcgcc agtcaacttg aaaaagagag
    15061 gatgagctca ctactagcta caaaaattga ctcacttatc aatcaataca tcttaacaca
    15121 tcaatttgat gctcctaaaa gcatatttga tcttagtcgc ttggtaaaaa caaaagcttg
    15181 ccattacaag ggaactgcta tatcagctat tatgcttggt agtttcttaa gtgggggacc
    15241 aaaataaaaa tacaggctgt ttgacctgta tttttattta tcataatttc ttcggcgaat
    15301 tttaaaatca gagctaataa cttcgtccac atcaataatg gagataaagg catctggatc
    15361 caaaccagct aaaatacgtt tgacttcccg aacttcagtt gggttcaagg tcacatacat
    15421 gatattgtaa tcattaccag agtaagctcc ttgacctttc aagtaagtca ctccacgatt
    15481 gatttcggtt aggatagctt gagcggcagc ctctgaatgc ttggtaataa taatcattcc
    15541 acggatagtg taaccaccat tttgaaccac actaatcatt tggctaaaaa caaagcttgc
    15601 caccaaagta taaagcatgt gttttaaatc cacataagtc aacgaagctg tcaatacaag
    15661 agcatccacc aaaagcaaag tttgacctag cttagctcca aatttttctt ctgcaatgcg
    15721 accaataata tccgttccac ctgtggttgc accatagcga aaaacaatgc cactcccaat
    15781 ccctgaaaag agacctgcca ctacagccac caacatcatg tcattttcca agccaagttc
    15841 gataggaact ttttgccaca tccagataaa agcggacatc agaactgttc catagatggt
    15901 tagagccaaa gaacgcttcc caaaaatctt tgcccctaaa ataaaaagtg gaagattaaa
    15961 taagagagaa gagtaagcag gattaatccc aaaatgcgcg tgtaaaatca aggttattcc
    16021 tgccacacca ccttcagcca aagcattagc catgttaaaa ttaacaaaac cgaaggtata
    16081 aatggctaca cctagggcaa tcaaaaacag ttttagtagt ttagtcaatt tatcgtgatg
    16141 tgccatcctt tatcctcact attcttcgca ggcaactcta atgctcattt attttctctt
    16201 tttatggtat ttgcttttaa tagttaaaaa ttctgggcta ttgacttcat taacatcaaa
    16261 ataagtgata aaggcattgg gatcaacctg agttaaaaaa gcttttaatt ccctaatatc
    16321 tcttcgcccc aaagccacat aaatcacttt tttacggcga ccagagtagg ctccttcacc
    16381 tgttagataa gtaactcccc ttcccaattc gtccatgatg aaccgggcaa ttttctccgc
    16441 ttgttctgaa acgataaaaa tgccacgggc tgaataacca ccttgttgta ccaagcgaac
    16501 aatttgacca tacataaagc tagcaatcag agcatacatc atttgcggca gggaaatata
    16561 agtcaaagag acaatcatga caaaaagatc aatgcctaaa agagcttgat tcagctgtag
    16621 tcctaacttg tcttctaata acttagcaat aatatctgct ccaccgatgg ttccaccatt
    16681 gcgaaacaca agaccgccac ctagaccacc agtaattcca gccatcaagg aaacgactaa
    16741 attgtcatga tccaagtcaa cgaccaaagg aaagcgttga aagagccaca caaaaaagta
    16801 aagtgacaaa atacccgtta ctgtgtaaac catggctctt ttcccaaaac atctagctcc
    16861 caacaacacc aaaggcaaat taatcaaata gcctatcaaa gaaggatcaa tctcaaaaag
    16921 tgccttacca actagggtga gaccagctaa accattggct gcaatgcgat tagccatgta
    16981 aaaataaaca aaagcaaagg cgtagatagc agctcctaaa ataataaagc aaaatttctt
    17041 taactgtaaa cgttggtcaa ttgacatgac acctcctgtg tttttcttaa tcatcttcca
    17101 caaaacgacc gataattcta acattctcag ccactgaaac aaaggcttgt gggtctgttt
    17161 ttaacatcag ccatttgaat tctgggtatt cttcacatgt caaaatggtg atcaaaactg
    17221 ctttttgttc atgcttgtaa gtcccttctg cgtcgttgat actggtcacc ccgcgatgca
    17281 gacgtttatg gatcatatga atcacacgct ctggatggct ggtaataatg gttgcctgca
    17341 ttttcttttg cttggtaaaa atggcatccg taacacgact tgatacaaag atagagacca
    17401 tagaataaag ggcgtactgc cagccaaata aaatccctgc aaaggctaaa attgcaccat
    17461 taaccatcaa tgagagactg cccacatcct tgcctgttcg ttttctaagg gtcaaactga
    17521 caatatcagt ccccccacta gagatacgtg atttgagacc tgtaccaatt cccattccca
    17581 taaccaagcc accaaaaata gcattgataa gaggatcagt cgtcagcgtc acttgaggca
    17641 tgatttgaat aaagaaagaa ctcatgctga ctgtgataaa ggtaaaaatg gtaaattgat
    17701 gcccaatttt ataccaagct aaaataagca aaggaatatt aatcaaataa aacgcaaagg
    17761 cgatgggaat atcatagcct aaaagacgat gactaagagc tgaaaaaacc tgtgctagac
    17821 cagttgctcc acttgaataa acatgcccag gctggaagaa aaaattcaca gcaatactag
    17881 agagaatgcc ataaagcaga gaagccgaaa tcttctctgc atatttttct cttgaaatac
    17941 ttcttagagc gtggagtagg ccaactttct tggcccctct actaatgaca tatttaactt
    18001 ttttcttata ggttgttttc ttaatcatga ctttctattt gaagactgag ttcttctaat
    18061 tggttttcag aaacaagact aggtgcttgc gtcattggat cagaggcttt gttattttta
    18121 gggaaggcaa taacttctcg aatattgtct tttccagcaa gcaacatgac aaaacggtca
    18181 agaccaattg ccaagccacc atgaggagga aagccgtagt ccatcgcttc tagtaagaaa
    18241 ccaaattggt cgttagcttc atcagcggta aagccaagag ccttgaacat gcgctcttgc
    18301 atctcttttt gattgatacg caggctacca ccaccaagct cgtaaccatt taaaacgatg
    18361 tcgtaagcaa tagcccgtac cttagccaaa tcaccctcta actcatgagc agattctggc
    18421 gttggaagag tgaaggggtg atgagctgac atataacgtc cttcttcttc tgaccactca
    18481 aacattggcc aatcaaccac ccaaaggaaa ttaaactgcg actgatcaat catgtcaagg
    18541 tccttggcaa tacgattacg aagcgcacct aatgtattat tagcaacctc aagagtatcc
    18601 gctacaaata aaaccaaatc attctcagca agttttaatt ggcttgacaa ctctgtctca
    18661 atagctgtca agaacttggc tgctgggcct gccaattgac catctgtcac cttaacccag
    18721 gcaaggcctt tagcaccaaa ttgtttggca aactcagtta atttgtcaat atctttacga
    18781 gagtatcgat ctgcatttcc cttcaccaca atcgccttga cagctggtgc ttctgagaaa
    18841 accttgaagt caattccttt gacagttact gtcaagtctt gtaaaagcat ttcaaaacgt
    18901 gtgtctggtt tatcagagcc gtagctattc attgctacat cgtagctcat acgtgggaat
    18961 ggtagggtta catctatttc ttttgtctct ttcatcactt tagcaatcat gccttcaacg
    19021 atgtcttgaa tttcttgttc agaaaggaat gacgtttcca agtcaacttg tgtaaactca
    19081 ggttgacggt caccacgtag atcttcatcg cggaaacatt tcacaatttg ataatagcga
    19141 tcaaagccag cattcatcaa taactgtttg gttatttgcg gactttgtgg caaagcatag
    19201 aaatgccctt ggctaacacg acttggtacc agatagtcac gcgcaccttc tggagttgat
    19261 tttgtcaaca taggtgtctc gacatcgata aactcaaggt catcaaggta attacggata
    19321 gaatgagtca cttttgcacg tagcttgaag ttttctaaca tttctggacg tcgtaaatct
    19381 aagtaacggt aacgtaaacg cgtgtcgtca cttacttcaa cgtcatcttt aatttcaaaa
    19441 ggcgtcgttt tagctgtgtt taaaatggtc aaagcagata cttttaactc aaccattcct
    19501 gtcgccaact tatcattggc ttgttgtcga gcttcaacaa aaccttccac ttcaatcaca
    19561 tattcactac gtaagcgttc agcagttgcc atcacatcac tggaaacttc ctcagggtta
    19621 atgactaatt gcatcacacc ctcacggtca cgcaaatcaa taaagatgag accacccaag
    19681 tcacgacgac gactgaccca tcctttcaat gtgattgtcg ttccaatgtg ttcttcgcga
    19741 acacgccctg catacatgct acgtttcata aataaattcc ctaaaacaaa cagaatcctg
    19801 tgaaacctgc tcgtttccaa ttcctttcac tatataaatc aattaattca ttccctacta
    19861 ttatagcaaa aagcccttaa aaaccccacc catttaaaaa gagtttttct aaagataaag
    19921 caagaatact gagtcgtcag cattttgaca tatcaggtgt cctcacagaa tactaagaaa
    19981 gctaatcaat ccccttacag aaaacaaaca ggtgcaactc ccttctaaaa agagaagtta
    20041 cacctgtttt agtgattatc ttctaaacaa tcatatcaac acaagcttat gacgacaata
    20101 aaatcattaa gacaataatt gctctgagat gttttcaaag ttggtcatca tatcttcaaa
    20161 actgacttcc acttcttgac ggctacgatt attcttaatc acagctttgc ctgcttcaac
    20221 ttcactttct cctaaagtca ttaccaattt tgctttaaag gtatcagctg atttaaactg
    20281 agccttaatc ttacggccaa ggtaatctcg ttcagctgta aacccttgac ggcgaatggc
    20341 ttgaaccaat tctaaagctt tgctattagc accatcacct aacacagcaa ggtaaatatc
    20401 catctctgtc tcaattggca aggtaatgcc ctgcttttca ataatcatca agagacgttc
    20461 taaaccaaga ccaaagccaa atcctggagt ctcaggccca ccaaaataac ctaccaaact
    20521 atcataacgt ccacccgcac agatagttaa gtcagaacct tccacagacg tgatgaattc
    20581 aaaaatagtg tggttatagt aatcaaggcc acgcaccata ttggtgtcaa tcacataagg
    20641 aatgtcaagt gcttcaagca tatctttgac cgcctcaaag tgcgcctgac tttcctcatc
    20701 taagtaatcc aaaatagaag gcgccttttc aacggctagt ttatcttctt tttctttaga
    20761 atcaagcacg cgcagtgggt tttcatccaa gcgacgttga ctatctttag ataattgatc
    20821 acgcattggt gttaagtagt caatcaaggc ttgacgataa gctgcacgac tctcaggact
    20881 ccccaaacta tttaaatgaa gggtgacatc cttaatgccc aatttttcaa agaggtgata
    20941 ggccatcgca atggtttcaa catctgttgc tggattagca gcgccgaaac attctacccc
    21001 aatctggtga aattcacgta aacgaccagc ttgaggacgt tcgtagcgga acatggaacc
    21061 gatataataa agtttgacgg gtttttggac ctctggcgca aacaatttat tttcaacata
    21121 agaacgcacc actggagctg taccctctgg tcgcaaggta atgtgacgat ctcctttatc
    21181 atagaaatca tacatttcct tcgtcacaat atcagtcgta tcacctacag accgcgagat
    21241 tacctcgtag tgttcaaaca taggagtacg aatttctcca taattatatt ggctaaacgt
    21301 atcacgcgca acagattcca catactgcca tttcgcagca tccccaggca gaatatcctg
    21361 ggttcctttt ggtttttgaa gtttcataag tttacctctt tgattagttt tctctttaaa
    21421 cattctacca ttttttctta tattttaaac aagcatatat catttgcttt agtttgatag
    21481 tattttcacc atgaaattgt aaaaataaaa agttttgtca agtaacttta ctttacaaaa
    21541 aagatatgtt atcatagtga agttgatgaa aatcaaaact tacacatcga aaggaagaac
    21601 aagaaaatgg cagtacctgc acgtcacacg tcaaaagcga agaaaaacaa acgtcgtaca
    21661 cactacaaat tgacagctcc atctgtacaa ttcgacgaaa ctactggaga ttactcacgt
    21721 tctcaccgtg tatcccttaa aggatactac aaaggacgta aaatcgctaa agctaacgaa
    21781 gctaaataat agaagggaga taccatgcgc gtaaatatta cacttgaaca taaagaatct
    21841 ggtgaacgct tgtaccttac ttcaaaaaac aaacgtaaca ctccagaccg tcttcaattg
    21901 aaaaaatact caccaaaatt acgtaaacac gttacattta cagaggttaa gtaaggggtt
    21961 cactacacgt cattagacgt caaaacgttg attttaagcg atttttgaat ttttgaagtt
    22021 cattacattg taatataaat caataagaac tctacctttt actctacctt ttttagatga
    22081 atatatcatt tggatattga agttaagccc cgttatcatt tggatggcag ggctttttta
    22141 tatttggtac actaacttct tatctatatt ttggtgtagc tgaaattgac atctacaaag
    22201 tttaatgtta aaattcattc aaaaatatat ttgaatgagg tgttttatga ttcaaaatgt
    22261 tgttacttca ataatcctgt attctgggac agccgtagac ttacttatta tcctaatgtt
    22321 attttttgcc aaaagaaaaa gcagaaaaga catcattaac atctatttag gacaatttct
    22381 aggctctgtt agtctaatat tgctaagttt gctttttgca tttgtcttag attatattcc
    22441 tagtaaagag attttaggtt tgctcggttt gattccaatt ttcctaggcc tcaaagtttt
    22501 gcttttagga gattctgatg gagaagctat tgccaaagaa ggtttgagca aagataataa
    22561 aaacctgatt tttctagtcg ctatgattac ttttgcaagt tgtggtgctg acaatattgg
    22621 tgtctttgtc ccatatttta ctaccttaaa tttagcgaat ttgatagtgg ctttacttac
    22681 ctttctagtc atgatttatc tcttggtttt ttctgcccaa aaattggcac aagtcccttc
    22741 tgttggagaa actttggaaa aatatagcag atggtttatt gccgttgtct atttaggatt
    22801 ggggatgtat atcctgattg aaaacaacag ctttgacatg ctatgggctg tgttaggcta
    22861 ggagaaaaaa ttatgaaaaa agatagtatc tgtcaagtgg gtgttataaa tcaacaaaat
    22921 gttacaaccg caacgaacta ccttgaaaag gaaaaagtcc aaaaatcact tcgtatttta
    22981 tcaaaattta ccgataataa acagataaat atcatctttt atctccttgc cgtcgaagaa
    23041 ctctgtgtct gcgatatagc ctgtttatta aatctcagta tggcatctgc ctcccaccat
    23101 cttcgtaaac tatccaatca aaacatcttg gacactagaa gagaggggaa aattatatat
    23161 tattttataa aagatgagga aatcagagat ttttttaatc aactaggata acaactattt
    23221 ttactacttt ctacttttcc atgattatag ggggattata tatgaaattt tttgaagaaa
    23281 attattcaca agaaatacct actcgtatca aatgtttaag aaaaaagtat aatttaaagt
    23341 aaagtgacct aggtaatgca ggtcaagtta gccaagttga aaagggaaag cgacaagtta
    23401 ccgcttcaat attgctttat ttgaatactc aaaccgactc ggactataaa gaaattattt
    23461 ttggagatat tgccaagttt gttgaaaata tgttttacca ctacttcagc tctattttat
    23521 ttagagattt ggaaactgta gataaaagaa tgtattcttt ttcggacgat gacttaattt
    23581 caatacaatc cagctgtctt agactttcca agacattcgc aaatttcaat attcaaagaa
    23641 aaaatttttt ggcttctgat gaaactgaaa tggatacttt tcacaagaaa gatgacattg
    23701 acattactgt tggtgaaaaa agttataacc tcgctagaag ttttagaacc agtaccatca
    23761 atgaattgac tgtctttaat tttgaagaaa tgtttgatat tatttggttg atgttagggg
    23821 ataatttaat taaatcattt gaagttaatg tctgtggtat cctatttgaa ttaaacggaa
    23881 atggcatacc ctcaacattt aggcaagaaa acattgatcc cctcatcaat aaatggtggt
    23941 atgataatgt ttcgacagaa attatcccta acctaataaa aaaactcaaa gaaaatccac
    24001 tatttaatat cggattcctg gttgatgata ttctagaacg aatgtataaa gaaaatatcc
    24061 caaaatctta ccttacgtcc gtcccgttag ttatttccaa aaaagcaagg tcaactttat
    24121 cgattaagtc aaacggaagt caaaaaatag ataaatcaaa atttgcacag attttgtccc
    24181 tttagagacg gatgacttac acattcaacg tggattattc ttcccttgga gaaaggggat
    24241 ttcagaacgc ttggcgattc ctgatttaga taaattttca tctagcttac aacaggggaa
    24301 aattcctgtc ataaagcact tcgaactcaa tttagataag gggagtcact gggctattgc
    24361 tggaaattca ggttctggga aaccgtacgc tctcacttac ttcttgagtg tcctgaaacc
    24421 tatgtccggc ctaattatca ttgacccaaa attcgatacc cctagccaat gggcaagaga
    24481 aaataaaatt gccgttatcc acccagtaga aaaccattca aaatctgatt tcgtttcaca
    24541 agtcaatgaa caattaaacc agtgtgcaac tctcattcag aaacgacaag ctatcttgta
    24601 tgataatccc aatcatcaat ttacccatct aactattgtt attgatgaag tcctcgctct
    24661 atcagaggga gtcaataaga atatcaaaga agcttttttc tccctactat cacaaattgc
    24721 cttgttggga catgctacta aaatccatct attcttggga agccagcgtt ttgaccataa
    24781 cactattccc atttcagtga gggaacagct aaacgtatta ttgcaaattg gaaatatcaa
    24841 tcagaaaacc actcaattct tatttccaga cctagatcca gaggggattg tcattccaac
    24901 aggacacgga acaggtatca ttcaagttgt cgataatgaa cactcctatc aagttctacc
    24961 cttactctgt ccaacctact atactaagcg aggtatccta tgaccactaa aacctacttc
    25021 atgagaagct tcaatctcat attatactta ctgcttattg ctccaatctg ctatgtgttc
    25081 ggttggctgt tcaatcttgt ttcagttcaa ttatctaccg tcacatttgt caatctggat
    25141 acggttaaaa acgttactga taccattagt tctgtatgtt atggattcgc tcttatttct
    25201 ttacttctag ccttattatc tggattaaag atgatacaac gcttgaatac agatagcctt
    25261 gggaactact gccaatctat ttatcgcacc tttctattaa gacacttcct tttccagcgt
    25321 gaacgagtac agaaggtaag caactttgaa caccagaccg ttaccactat caatccaatc
    25381 cataacgggt ttaaccgagc tgtacgtaag tgtatcgtag atatacgaaa agatgccatt
    25441 actgtatttc taaaagttcc tagagaccaa caggggcaaa aaatcctaaa ggatacggga
    25501 gtacaactga aagaagagat tgtcatccaa cacacagact attatttctc ttctcctgtt
    25561 cgagttcgta accaattgtg gtttattggt caaaagcgtt aattccttgg ggctgtggct
    25621 tgcgttaagc aaaagccaga cagccccttt tttatattct tatctatcat gacctattgg
    25681 ggttactacg acaacgaaag ttagaagctg tgggtattta tttatcttga aaagattgct
    25741 gaactaacgc aataactact ctaccattca ctctaccttt tattaagcaa ctatcaataa
    25801 atattgatac aataaggttt ataacagcta aaatagaaaa tacttaacct ttacagaagt
    25861 aaaataattg agatgagaaa agcctatgaa atgaacgttt caaaggcttt tttcgtgttt
    25921 aaaaaaacaa atgaaaaaac aaaatagaga gtagggttat taacactgtc aatcctgtgt
    25981 acgcaataac aatccatgct ttttgataag gatattttgc tttttcttta atcttatcaa
    26041 tctgatctaa gctctcaccc tttgctatag cttcaagtta tcggtaagtc tgaacatcat
    26101 atgtcttttt aaatcgctca attcttaagg cgatacgcat gattatcaac cataagatag
    26161 caaccggacc atatccccac caacctaacc aagacattat aggataaata ctgatagcaa
    26221 ttaggagtgt cccaaataac ataatatgcg tattcctatc acattgttta atctcctcat
    26281 ccttaataat taaagtcatt tttgggatat cttctgctat tagttgctct aaaggaacct
    26341 gtaaggactt acttaacatc aaaagatttt gcaggtctgg atagctttta ccgttttccc
    26401 agtttgagat agtcttcttg aaacatacat catttcagca agctcttctt gtgacatctg
    26461 taattttttt caataataag gtaactggtt tttaaagccc ataataaatc tcctttttaa
    26521 tagctactat aatccttctt caataagttt actatcaaaa gtctttgacc taagcttttt
    26581 ttcgtttatc tttttgataa ttatcgtaag gtttacagtc tttgtaaaat ttatttacaa
    26641 ctttgacaat caaacttgac ttgatcacaa agacatggtg tttaaggaaa atatcttcaa
    26701 aattgaatgt attatttatt aggaacaatt attttagtag ggatatgcag ctttgtgtca
    26761 gcaattcttt aaatttatga tacaataaaa tcattctttt caggaggtaa caatggtttc
    26821 atttatttca agagttttta aaggcatgat tattgcacta ggttttattt tacctggtgt
    26881 atccggtggt gttctcgcag ccattttagg aatctatgag cgaatgattt cctttttagc
    26941 tcatatgagg gataacttta ttgaaaatgt cttatttttc ttaccagtag gaattggtgg
    27001 tattttaggt attgctctct tttctttccc cgttgagttt ttgcttaagc actatcaagt
    27061 tatcgtctta tggggatttg ctggcgctat tgtcggcact attcctagtc tgattaagga
    27121 gtcaaccaag cagtctcaaa gagacaaagt tgactggcta tggctagtcc ttacctttgt
    27181 tacttcagga ctaggtcttt acttcttaaa tgacttaatc ggtaccttac cagctaactt
    27241 tttaaccttt attttagcag gtgccttaat tgctttagga gtactggtcc ctggactaag
    27301 cccgtcaaat ctcttactta tcttaggact ctatggtcct atgttaatag ggttcaaatc
    27361 tttggattta ttagggactt tcttacctat tgctatcgga ggagtcctag ccatcttggc
    27421 tttttctaaa agtatggatt atgctttaca gcaccatcat tcaaaagttt accactttat
    27481 cattggtatt gtattatcca gcacgctatt aattcttatt ccaaatagta gtagtcccga
    27541 atccatttcc tacagtcatg caggtatctt aacctggctt atgacctttg tgttatttgc
    27601 tcttggtatt tggctcggac tttggatgag tcaattagaa gaaaaataca aataaccttt
    27661 acaggttgtt gacaaaaagt tgacgccaac aaactagtgc tctatctccc cttagatttt
    27721 tcaatatcta aggggttttt ctttgcaatt gattcatcat ttagaatatc ggttaaacac
    27781 ttagtaaact gggcagtcat tccccatatg gtttcttcta gattttcata aaacggaata
    27841 ctacggcact cttgactaaa ttggtagtat ttcccattac gaatacggtc aaaaggaaaa
    27901 tccgttgtct caataggagt ggcttctaaa tgataataga caggatcagt gacaagtagc
    27961 tgacgtaaag gtactgtaaa gaccttgtca acttcttcgt taggctgtat gctttcccag
    28021 tcatcaacga ctaattgacc aacgaaacaa tgaatagttc tagaagactg tactaaataa
    28081 tcaatttcac cccatagctg gatctgggac gcatcaacag tcaattcctc aactgtttct
    28141 cggatagcag cttcttgaag tgtctcctga ttttctactt gtccccctgg gaaagaaact
    28201 tcccccggtt gtgaaatatg ctgacttctg acttcatata agacatgcca gtcatcattg
    28261 acaagaataa gaggtaaaaa tacggcgtac cgcttctcct ctcctagtgg ttttgcctaa
    28321 tactgtttta ataagtcctt catagccttc cctaatttct ctttctgtta atcttatctt
    28381 catcataacc aatcctagct ttttggtcaa cttattactc ctagttagtg gtttttgata
    28441 tatattattt ttttatatat aaaatattga catatcaaaa aactaagctt atactaaata
    28501 tgtaaaataa ttatatataa aatttttata tatgaaagga gcctaaaatg tattttccag
    28561 tatcggctac cttgattgaa tttctgattt tagccattgt cgaaaaaaac gattcttacg
    28621 gttacgatat tagtcaaacc attaaactag tggctaatat caaagaatcc accctctatc
    28681 ctattcttaa aaaattgcag aaggcaggtt ttttgacaac ttatagtcaa gaacatcagg
    28741 gacgaaagcg taaatattat gctgtcactt cttcggggag agcacagctg atttttctaa
    28801 aaaaagaatg gcagagctat aaatttgcct tagacgggat tattgaaggg agcttaagac
    28861 atgacaagga ctgagtatct agcagaacta gataaatact tgcgcaaact acctagagaa
    28921 gactaccatg aagcgatgga atattatatt gaatattttg atgaagctgg tcctgacaaa
    28981 gaaagccagg ttatcgacga tttaggaaat cctaaggaag ctgccagtga aatcatttca
    29041 aatgttctag gaaaacactt ggcaactccc gagaaaactc ccaaaaatcg ggcaacaatt
    29101 attggattaa ccattttgtc cctctttgct gctccgattg ctttacccgt attgctagcg
    29161 ttgattctct ttattattgc ctgcctaatg ctcgggctca cagctatctt tgccgcctac
    29221 atttttggga ttgcaggtat tcttgtcgca ggagtgactc tgtttgaaag cttgaccttg
    29281 attggctcat ctattcctgc acaagctata ggcatcggca gcgccttgtt gtgttttggt
    29341 ggtggtcttc ttatttggat tattgccaca gcaatccttc gtttttcagg acgggctttt
    29401 gttgccttta ttaagtggat cagtaataag aaaggagctc aagcatgaaa aatatctcac
    29461 gcaaatgctt tatgaccagt gtggtatgta ttattctagg aggcattctt ctaggggctg
    29521 gctatgcaac tggaggactt caggacatta aacaccaaac agctcccaaa aaggtcatca
    29581 aaacatttga ccaaataact gctcttgata ttgacagttc tgcctcaact attacagtag
    29641 agacaggacc cgttcaaaga ccaacagtga cctattacac acatcctaaa tttattgatc
    29701 ctttagaaat taaaatagag aacgagacac ttcatatttc tgaaaaaccc agagatgtca
    29761 tcatcaccgg aggtatggaa ctgatcgggt ttggattaaa taaaagtgac cgcaaccaca
    29821 actaccgaac cactaccata acgcttccaa aaggtaccag tcttaaagag ctcaaaggct
    29881 caagcatgcc tcaagccact atatcggatt taacgattca agaccttgaa ttcagtggtg
    29941 gtctgacact tctaaatact agaataaaga aagggaaggt ttttggaaca tttgacgcta
    30001 ctgatagctt gttagccaat cttgagttga aagccgacta ttcgttgtca agccttacga
    30061 attctagttt agaaaatggg acgattagct tagggcatgg acaattaacc acaaaaaata
    30121 ctaatcttaa atctgtcacc atccaatctc tatctcctgg tggggttgaa gccgaagaaa
    30181 caagccttga aaatgtgact ttcactgtta ctagtgagca agaaaacgag gaaaccaatg
    30241 ataatggtgc tatattcact gctcatgctc ttacccttaa aggcactaat acgattagtg
    30301 gtggtgatgt tgctgttgac ataactttga ccaatccaaa gacgaccgcc tataagacaa
    30361 aaaccgaaga tggtaaaatc tcacttggat cacagctgac accagctaag attggtaagg
    30421 aatcaacttc agatgttatt tcttatgtgg ctgagaataa agcagctact ggaaatttaa
    30481 cggttaatct caataaggga gacattacta tcaaatgatc ttaacaagac aataaaaaac
    30541 ttgacagaaa ttgtcaagtt attgtcaaaa taagacaaaa aacagcatct agtgtaactg
    30601 ccttagatgc tgtttttttt gaggttattt tatttagact gttttcttag gacgataaat
    30661 gagtagtgct aataccataa atgccagtaa gaaaccagtt aagactttca cttctactcc
    30721 aatatgacct gataatgaaa tggtttgtcg taaaccagat accacataag tcattggtaa
    30781 gaaaggatgt aacttttgga agaatgctcc acttaactca atggggtaag agccacctga
    30841 agagccaacc tgaagcaata acatgaccaa agaagcaaaa gagccatatc gatcatccca
    30901 accgaccaaa gctgtgacaa gagccataaa cgtccaacca cttagcataa taaatcctaa
    30961 ggtttccata ccataacggg cttcaaaccc taataattga atagctaagt agagaacaat
    31021 ggatcccata gtcgaaataa aaccattaat aacaaatttt tgtttagccc aatcccattt
    31081 atctttgacc ggacgaccag ataaagaatt agcaaaaatg acgttggttg aaagggccac
    31141 aaccattaga gaaacagcaa tcatataagg tgccatcccg atcccattcg tcttaacacc
    31201 atctttgtct ttctcattta acacaagagg ttttgcgaca gctttagcat ttttatcagt
    31261 cacagaaaca agcgataact gctgagaggc tttggctaag gaaccattta aggttgacaa
    31321 tcctgttgat aattcagtca atccgctcgt taattgatga ccaccctgac tcaattgaga
    31381 actaccatca gctagtttag tagcaccctc tgataattga tggctaccat ctagtaattc
    31441 tccggacttg gatgacaagg cattggtacc ttctgagagt tttattaccc cagaaccaat
    31501 ggcagtatta tacctgttta attgtgctaa tcctgttgtc aattgattac ttcctggtag
    31561 aacttgttga tttaagctac catttacctt tgctaatccc gttgataatt cagttaaagc
    31621 agaacttgct cccggcaaag cttgattgga ttgattagca atctgtgcaa cagcttcctt
    31681 aagttgctcc aactgttttg actgatcttc ttgagagaga gactgtaaac ttgttgacaa
    31741 agtttgaaca gaacttaaaa tagtttgggc tgcagatacg gtctgacttt tatcagattg
    31801 actgagggcc gcagctaact ctccttgttg ttcagcagtt aatgattggt aaacgctagt
    31861 agcttggaga gccgataatt cttcattctg agcggcagtc tcttcaacaa tgacttgttt
    31921 ggcagcttga gcgatagctc ctaagctatt acctaactca tcagcattaa ggttaggggg
    31981 ttgcaatgtt gatagctctg tatttagttg ctgaatgcct tgatttagga ctggtagtcc
    32041 tacaattaag gattgaattc ccttagcttt ctcgtcagaa agtgttgttg cttgtgtcaa
    32101 ctgtgaaaga ccctgattaa gctgagaagc tccctgggat aacctagaaa ccccgttcaa
    32161 ataattgggg atgtccgttg ataatgttcc taacccattc ttgacttggt tgacaccatc
    32221 tgtataggta gtcaaacctg atgtcaatcg accagtactt tgttgaaact gttggctagc
    32281 acccgttaag gctgctaagt tcgtcgaaag tgtttgactc cccgcctgag cggtcttcgc
    32341 tcctgaagca agtgcttgac ttccagctga ggcttctttt aatcctgatt ggaggtctgt
    32401 catactgcta aaaactgctg aggtataagt cttcgtaatg ttttgcgaaa cagactcttt
    32461 cagcttagcc atcgctgttt cccccatctt agcagcgacc attccatgtc ctttactcgt
    32521 ttggtaacgg atagttaatt tttggggttc gggatttaat aaggttgctg cccgttgaga
    32581 aagatcttcg ggtaaagtaa taaccatata ataatcacct tctttaagtc cctcttgagc
    32641 ttgcttagct gagacaaaat gatactctaa atctttactt ttagacatct tgtctaccat
    32701 atcatcccca attgtcaatg acttatcagc tctctttgca ggcttatcat gattaacaac
    32761 agcaatggga aggtcattga cccgaccata aggatcccac attgagccta gaaaggataa
    32821 attatataag gcaggcacta gggccacacc aatcattgta atcattaatt ttggattttt
    32881 aataagtgtt ttcaattctt ctaacatatt ttctcctaaa atggacatgg tgttcaaaaa
    32941 ttatgataaa atatattata caatttaaat ttaaatttac aagataaaaa atttcacttt
    33001 tggacattat gtctaaaagt gttcaaaagg agagatggta gatggtcaac cgtaaagaaa
    33061 atacgaagca agcaatttta aaagcaatgg ttatgttact aaaaaccgaa agttttgacg
    33121 atattaccac agttaaatta tctaaaagag ctggaattag tcgctctagt ttttacacac
    33181 actataaaga taaatatgaa atgattgact attatcagca aacctttttt cacaaattag
    33241 aatatatttt tgagaaaaag tatcaaaata aagaacaagc cttccttgaa gtatttgaat
    33301 ttctccaaag agaacagctt ctctcttccc ttttgtctgc taacggtacc aaagaaatac
    33361 aggcctttat catcaataaa gttcgcctac tcatcactac tgatttacag gacaagttcc
    33421 gcaccgaaga gttatctcaa acggaaaaag aataccaaag tatctactta gcccatgctt
    33481 tttttggtgt ctgtcaaagt tggattgcta aaggtaaaaa agaatcacct caagaaatga
    33541 cacaattcgt tctcaaaatg cttacgagta cctaaatagt aacccttacg aaagtatcat
    33601 tttacaagtc atcagcctta gtgataactt ttttagaagc catagtaaaa tgaagggatc
    33661 agaatagatt tgttatgaga tacaaaaaaa gcctattaaa taggcttttt gtaagtttaa
    33721 tcttaaaatt aaagcatttt gttatagaat tcaacgacaa gtgcttcatt gatttctggg
    33781 ttaatttcat cgcgttctgg aaggcgagtc aatgaacctt caagtttttc agcatcaaat
    33841 gatacgaaag ctggacgtcc aagagtagct tcaacagctt caaggattgc aggtactttc
    33901 attgattttt cgcgaactga aatcacttga cctggatcaa cgcggtatga agggatatca
    33961 acgcgtttac cgtcaacaag gatgtgaccg tggttaacga attgacgtgc ttgacgacga
    34021 gtagttgcta aacctagacg gtaaacaacg ttgtcaagac gacgttctaa aagaaccata
    34081 aagttgaaac caagagttcc ttctttgatt tttgtagctt gaacgaacaa gttacggaat
    34141 tgtttttcac ctaaaccgta tgagaaacgt agtttttgtt tctcagctaa ctgcaaacca
    34201 tattctgaga gtttgctacg gttgtttgga ccgtgttgac caggaacgta gttacgacgt
    34261 gccaattctt tacctgtgcc tgtaagtgat aaaccaaggc gacgtgattg tttccatgat
    34321 ggaccagtat aacgtgacat attacatgtc ctcctctaaa ttttttgagg aaataacggt
    34381 ttaaaaaatc ctgattcgtg cagggggttt tcgcctaaac agcaaaggtt acttgaatag
    34441 ataaccacct gttgacgagc ttcatatttt tctgctgtta tttcgcacaa agaacagttt
    34501 accataagaa agttgttttg taaagtcagt ctatctatca tctgatgatc agtttaatat
    34561 tttaaggtca gatatgggac gagccaccac tgatgaatta agaagactat attataagaa
    34621 gttatagtta tggtaataaa gacaactgtc attaacaaaa aataatttaa atgctaattt
    34681 gcagttctta aaccttgact gttttttttc ttagctctct atcaaaagca cttcactgga
    34741 aactcttaat tgctctgaaa tggctagtat attgtattta gtcaggggag gtctactcgg
    34801 tttttcactt caagatagtt ttaccggata cttcaagttc ctctattaat tacacttgtg
    34861 tcaaaacttt atttcacgat aatcttttaa ccttaatact aagatcattt gcttaaccat
    34921 ggtgaagacc tcttttttac ctcaatctaa cctaaaaata ttatctttta taggaatttt
    34981 ttcaaaataa aaagaataca ggcagaataa tcttctatcg ctaataatta tatcaattta
    35041 ttgtaaagaa aaagcctcta tgaggcaaaa atcaatctaa ataacgaatt aatgtttttt
    35101 ctgtcaaaat atctgaatag gtgtatgatt cgacataaga attatcaatt gctcctggag
    35161 tatcagagct atcactatac tcaatgataa agagtgaact gtaaacctca ataagcctac
    35221 caattttatt tttttcccgt ttccgtccat tctcaagggt taattctact aactgtcctt
    35281 catgggctct tatgtcctct ttaatttttt tcatttttgc aacatctgta aatgcatcgc
    35341 tcattttttg tctctctttc ttatctttct tcaaactggg ctatacttga gaatttgttg
    35401 tattcttttt ggaacatcag tttgactgtt ccacgcgccc cagctctatt tttctcgagg
    35461 ataacttcaa ttgtgttatc ttcaacagct tcttcagcat catcaccttc tttacggtaa
    35521 taatcgtccc ggtataagaa ggctacaata tcggcatcct gctcaataga tcctgattca
    35581 cgaatatctg ataaaactgg tcgtttatct tgcctttgct caacgccacg agaaagctga
    35641 cttagggcaa taactggtac tttcaattct ttagctagga ttttaagctg tcttgaaata
    35701 tctgaaacct cttgctgacg attttcgggt ttagttcctg taatcaactg taagtagtca
    35761 attacgataa gacctaaacc accatccact tcttgagaca atttccgtga tcttgcgcgg
    35821 atttcagtaa ttttaatccc gggcgtatcg tcaatataaa tcggtgcttc tgccaaagct
    35881 ccctgagcaa ttgttacatt attccaatcc tgatctgtga gttgccctgt tcttaaactg
    35941 tgcgaatcaa ccattccttc tgctgcaagc atacgatcta ctaaactttc agcacccatt
    36001 tccaaagaaa aaatagcaac agtctttttt tgcttagtcc ccacattttg cgcaatatta
    36061 agaacaaagg cagtcttccc cactgctggc cgagcagcta aaataactaa ttgatctggg
    36121 tgtaaacctg ttgtaatctt gtcaaggtct ctaaaaccag ttggtaaacc tgtaacattt
    36181 gaagtctgct tagaacgtgc ttctaaagcc tcgtaattaa cttttagcac atctgaaatt
    36241 ttgcgaaatc cactacgatt actatgttca ttgagttcaa ttaaagctct ctcaactcca
    36301 gcgataacct cttctggttt taaaatttca tcataagcta ggttgacaga ttctgtcaac
    36361 ctagcaataa tatcacgcaa catagctttc tcagctacaa ttttagcata atattctgca
    36421 ttagcactag ttgggacact attaactagt tcaacaatat aggataagcc accaatactt
    36481 tgcagatcat cttgatcatc taggattgtt cttatagtgg ttgcatcaat ggcatcatta
    36541 cgatcgctga gggtaatcat tgcccgaaag ataattttat gagcgtactt ataaaaatcg
    36601 tctggactga taaattctct cactgcaatc agcttatcag gtgagataaa gattgaccca
    36661 agaacagatt gctccgctag taaatcttgg ggttgaactc gtaattcagc tacttcaggc
    36721 aacctcataa cctccaaatc ttgtgatgtt ttttatatta tgcttctgtg atagccaatt
    36781 taatctcagc tgttacttcc ttatgtaatt tcacaggaac ttcaattaga ccaatggcgc
    36841 ggataggatg atctaaaaca atatgacgtt tatcaacttt tacaccaaat tgcttttgca
    36901 attcttctga aatcttttta gctgtgatgg aaccaaatgt acgtccatct ggaccaactt
    36961 tttcttgaaa ttgaacacgt gttttgtcct catccaaaac agccttgaca gcttgagctt
    37021 ctgctaatat ttcagcttga gctttttctt cagctttttg ttttcctttt aattcaccga
    37081 tgctttgact agttgcttct ttagctaaat ttttcttaat aaggaaattt tgagcatagc
    37141 ctgttgggac ctctttgatt tctccttttt taccttttcc ttttacatct gctaaaaaaa
    37201 taactttcat gattctactt ctcctgtttc tttcattgtc atattaatag tttttaataa
    37261 taagtgcttc gcttggggaa gactgatatc cgttaactga caggcagcaa gattaaagtg
    37321 gcctcctcca cctaattttt ccatcacacg ttgaacattg attttactac gacttctagc
    37381 tgaaatagca atcttatgag aagctgtttc aactaataca aagctagcct caacgtgagc
    37441 cattgacagt atcgtatctg cagctttact agcaataaca ttgctataaa gatgattttt
    37501 ttctcctgca gctacaatga tactgtcacc aagacgttct ccttgtaaaa taatttcatt
    37561 tatttgctta tattcttcaa aatctgttgc ggatatattt tgaatttcaa cactgtcact
    37621 tcctttactt cttaagtaac tagcgacatc aaaggtacga cttgtcactc gcgtagaaaa
    37681 atttttagta tcaagcataa tacctgccat taaaacactg gcttggatct tatttaaacg
    37741 ttttttagca ttttgaaatt gaattaactc agtgacgagc tccgcagcac tacttgctcc
    37801 actttcaata aaagttaaga tagcattatc aggaaaatca tcatctcttc tgtgatgatc
    37861 aactacaata acattttgaa actgttcata aaattcttta gaaagtgtta aagatatctt
    37921 agaatgatca accattacta aaagtgatct tggagtcact aaacccattg cttgagaaac
    37981 actgattaga cgagtctttc cgtcagcctg caatcgctca atagcccttt caatatctgg
    38041 actcatctca tctggattgt aaacggcaaa actattttca ataatgttac ctgcgaaaaa
    38101 ttgcatacca acagctgaac ctagtgcatc catatcaagt ttcctatgtc caacaataaa
    38161 aacattgtcc accatcttga ttcgatctga aatagctgtc atcatagcac gggtacgtgt
    38221 tcttgaacgt ttaactgtag aaacagaccc tcccccgaaa taaattggat tcgtatgatc
    38281 cgcattttca cgaatgacaa tctgatcacc accacgaaca agcgcaatat taagattctc
    38341 aagagctacc tgtccaatct ggctatgatt ttcttcacca aaagaaattc ctatgctgag
    38401 tgttaaaggg cgctgagcat cttgagcttc tttacgaaac tcttctaaga cagaaaattt
    38461 attatccatc aaatcattta atgttttaaa gtctgtaaag aaatagtagc gatccatatt
    38521 aacacgacgg taaaaaatcc gttttgactc cataaactca tcaatgaagt tagctacaaa
    38581 tgaattaatt tttgaggtat ctgcatctga taaatcatca gtgatatcat cataattatc
    38641 aaccgaaata atgccaacaa ccggtcttaa catactagca tcagctaatt gcctgtttcc
    38701 cacaaagcta tcaaaaaaat aaaaaattcc tgatgataca tcaatataag aagtgtattt
    38761 attaccagaa acttcaaatg tttgagaaat atcttcacga cgtttttccg taataatttg
    38821 ctgaatcaaa ctgttttgta taaaaccctc ttcagttgta aaaattaatt ctgcataagg
    38881 attataccat tctacagcgt tggtctcttg atcaaattga actactccaa caggcatatt
    38941 atcaagtagc gtttttaggt tatcttctgt ttgttcattt aagagctcaa tatgagctaa
    39001 gtctgataat tgataagctt ctttttgata ccataaaaga gcaacaacaa atagcaaaac
    39061 gagaaaaata gctaataata ttaatatttt tgattgcata atactgacac aaagtgccag
    39121 tagtccaaat aaaatcaatc ccatcataat caaatgaatg gtttcaaaac gaaatttttt
    39181 catcttataa cctcttaagc acacattata ccataaaaaa gccctgaaat acagagcttc
    39241 cacttgcttt gagattacaa tttcatttcc atttaatatt ttcgatgagc ttttccgttt
    39301 ccttctaaat aaatcatcaa gatactgatg tccgcaggat tgactcctga aatacgacta
    39361 gcttgtccaa ttgtttcagg attaattttc ttaaatttct gacgcgcctc agttgcaata
    39421 gaatcaatag catcccaatc aatatttgca ggaatacgtt tttcttccat gcgtttcatc
    39481 ttagcaacct gatctaaagc tttgcgaata tagccttcgt acttgatttc tgtttctaaa
    39541 agttcaatga ttttagcatc taaatcttca gccacataac caacaaaaga gactgcagtc
    39601 gcataatcaa tttctgggcg acgcataaat tctttagctg tcatagcatc cgtcaatggt
    39661 ttaaaaccaa gctcctgcac acgatcgttg gttgctttta ctggttttaa tttgatactg
    39721 ttcagacgtt ttaattcgtt atcaaattgg tttttcttaa tttcgaaggc cttccaacgc
    39781 tcatcatcaa ccaacccaat gtcgcgacca atctctgtca aacgcatatc agcgttgtca
    39841 tgacgtaaaa tcaaacgata ctcagctcgc gatgtcaaca atcgataagg ttccaaggtt
    39901 cctttggtaa ccaaatcgtc aatcataacg ccaatataag catcgctccg tttcaaaata
    39961 agttctggct taccttgaac tttcaaagca gcattgattc cggcaataag tccttggcca
    40021 gcagcctctt catacccaga agttccatta gtttgcccag ccgtaaagag tcctgaaatt
    40081 agcttggttt caagtgtggc acgtagctgg tgaggtaaaa caatatcata ttcaatagca
    40141 taaccagtgc gcatcatttc agccttttca agtcctttga tcgaatgaat caaatctttt
    40201 tggacatctt ctggaagact cgttgacaac ccttgaacat acacttcttc tgtatcacgc
    40261 ccctctggct ctaagaaaag ttgatgacgt tctttaccag caaagcgaac aatcttatct
    40321 tcaatagatg ggcagtaacg agggcctaca cctttaacaa tgccagaaaa catcggtgct
    40381 ctgtaaaggt tctgattaat gatatcatga ctagtttgat tagtataagt caaccagcag
    40441 ggaatttgat ctttgaggta atcagcatct tttgacataa atgaaaaatg attaggttta
    40501 tcatcacctg gctgaatttc cgtttgatcg taattaatag aagatgcttt gacacgagga
    40561 ggcgttcctg ttttgaatct gccaatttct aagccgagtt tttttaaatt atctgctaag
    40621 gttactgatg ccaaactatt attaggccca gaagaatatt tgagttctcc taaaataatt
    40681 tcgccacgca gagctgttcc tgttgtcact acaactgcct tagctgcaaa cttttgtcct
    40741 gtggctgtta aaacacctac aactcgtcca tcttcaacta gaatatcatc aatcatcgtt
    40801 tggcgaagag tcaaattagc ctgtttttca accgtatgtt tcatctcacg cgcatacagg
    40861 gatttatctg cttgagctcg gagagcgcgt acagctggtc ccttacctgt attaagcatt
    40921 ttcatctgga tataggtttt atcaatattt ttacccattt cgccgccaag agcatcgatt
    40981 tctcgtacta ctatcccttt ggcagatcct ccaatagaag gattacacgg cataaaagcc
    41041 aacatatcaa ggttgattgt tgccaataaa gttttacaac ccatacgact agtggccaaa
    41101 ctggcttcaa caccagcatg acctgcacct atgacaatga cgtcatagct ttctgtaaat
    41161 tcatgtgtca tttttttaaa atcctttatt tcagtctatt catttaaaat catacgcttt
    41221 agtttagcct tcaatgcgga caatatgtcc tggccattta gccaactcgg tttgtaaaaa
    41281 ctcaggaaca agattcattt gatccagttg attgattgga atccattcac aaacctgtcg
    41341 cttgcattct ctatcatttc tagtttaggg tcttctaagg ggcttaccaa ataatgaaat
    41401 tcaatattat gccagaaaac atcatcgata tcaaaatgat tctcaaccat aaaagccagt
    41461 tgattaacct tagctcgaat accaacttcc tctaaggttt cacgtaaaac agtttcatgc
    41521 gttttttcac ctaccaaact tgtaccacca atggtgtaat attggtcagc tgcatctcta
    41581 gttaaaaaat tcttaccatt tctaacaatc aagcgctagc tcgtaaacta aagttatctg
    41641 acctctagtg attttaaatt ccagtcctat tagccatctc atttctagag ctaaaccata
    41701 aaagggccaa aaccctagaa gattgcagga caaaaaaacc tacaactttc atgagctttg
    41761 accatcattc cgattgttac tatctatttt aaaaaaatga ttagtgattg tcaatattga
    41821 tttcatcaca gacctttaca gtaatgtcaa tgtttttaca gctctgtcaa tctattgtaa
    41881 tgtttttaaa atgattacaa aagtatcact atgtctaatc tagcgaggcg agaaataaaa
    41941 atgtcatcag tattatcata cgattttttg atatcttcta aagtagtaaa atacagatac
    42001 cgttgtttct aagtctacaa gtaagaacgc cgataacaaa accataaaag tataactaga
    42061 aatttccaat aattttttac acatttagta ttagataata ggtactaata atcatgaaat
    42121 aatctgtttt acgaaatacc taatcatcaa aatatctgtt gtaatactag agaaagtgag
    42181 tctcatgatc gtatgatttc tacagtaact aataaagtaa tgactcaact tttacgaact
    42241 atttttaaaa ataatgtgat tatatttaaa atatacagat atcataaatt aatccattaa
    42301 cgcatgattg gtttttataa ataatatcat atgatgtcaa aggaattgtt gaatgtccct
    42361 ttctttttat cttaataaaa gattcattaa atgtactgac aaggctgccc atttttataa
    42421 gccatgtcaa tcataccacc accaaggcat tcttttccgt catagaaaac aacagcttga
    42481 ccaggagtaa tcgcgcgctg tggctctgca aaaacaactt ctgccttatc tcctcgaaca
    42541 tgcaccgtca cgtgtgaatc gggttgacga tagcgaaatt tagcagtaca ttcaaatgtg
    42601 aactcttctg gcatctcacg tgtaaaatgg atgactgagg cgtcaagact atttgacatt
    42661 agggcttcat ggtagaaacc ttgaccaacg tataaaatat tttgcgacaa atctttaccg
    42721 acaacaaacc aaggttgatt atccccacca tgctgaccac caatgcccag acccccacgc
    42781 tgaccaatag tataatacat aaggccggca tgttctccca tatcgcgacc atcaatagtc
    42841 atcatgcgac ctttttgtgc cggtaagtac tggctcaaaa actgtttgaa attcttttca
    42901 ccaataaaac aaatacctgt tgaatctttc tttttagccg ttgcaagccc tgctcgctca
    42961 gcgatttctc taacttctga cttttgcaaa tgaccaaggg ggaataaagt cttttgtagt
    43021 tgttcctgtg atagttgact taaaaagtag gtttggtctt tgccattatc agctccacgt
    43081 aacatgtgaa ccgtaccatt ttcatcacgc ttgacttgag catagtgtcc cgttgctaca
    43141 taatctgcac ctaaagtcat ggcatagtca aggaaagctt taaattttat ttccttatta
    43201 cacatgacat cgggatttgg agtacgacct gctcggtatt cggccaagaa atattcaaat
    43261 actctgtccc aatattcttt ttcaaaattg actgaatagt aaggaatacc gatttgatca
    43321 gcaacggctg ctacatcttt gtaatcttca gtagctgtgc aaacaccaaa ttcatctgta
    43381 tcgtcccagt ttttcatgaa gacaccaata acatcataac cttgctcctt taaaagtaga
    43441 gctgttacag atgaatcaac gccaccactc ataccaacga caacacgtat ctttgagtta
    43501 tctgtcataa aaaattctcc catcaataat aagatagaaa aagataatca tcatattatg
    43561 aatattatga ttctttttct tatcagttaa cgtacgattg aaggtcgtcg ttttcaaaag
    43621 tcgatttgaa ggtcgatttt gaagcgccat cagcgtaaac cttattataa caattactgc
    43681 gtggaatgcc aatcatttta ttgctcaata attttttatt ataaaaataa ttttttataa
    43741 ttatattgct gttttttgat atttttttgt tataataaac atagaaataa aaaacggagt
    43801 tgcctatgaa tacacaaaaa tttcaatcag tttttgacat cattgggcca gtaatgattg
    43861 gtccatcaag tagtcacaca gctggtgccg tcagaattgg taaggtagtc cattccatct
    43921 ttggagatat tcctgatgaa gtgacgtttc acctttataa ttcatttgct aaaacctaca
    43981 gaggacatgg tactgataag gctcttgttg caggaattat gggtatggat actgataatc
    44041 ctgatattaa aaattctcta gaaattgctc atcaaaaagg gataaaaatc tattgggata
    44101 tcttaaaaga tagtaatgct ccccatccca atactgttaa aatcagtgtc aaaaaagctg
    44161 acaaaacact tagtgttact ggtgtttcaa ttggaggagg aaacattcaa gtaacagaac
    44221 taaatggttt ttctgtctcg ttatccatga atactcctac tatagttact gtacacaaag
    44281 atattcccgg aatgatcgca aaggtaactg atatcctatc ttccaataat attaatatcg
    44341 ccacaatgaa tgttacacgt gaatctgctg gtgaaaaagc tactatgatt atagaagtcg
    44401 attctagaga gtgccaagaa gcagccaacc aaattgcaaa aattcctcat atttataatg
    44461 tcaacttctt tgactagaaa ggaaagctat gttttatact attgaagaac ttgtcaaaca
    44521 agctgaccaa caatttaatg gaaatattgc tgaactcatg attgccacgg aagtggagat
    44581 gtctggaaga aatcgagaag atattatcaa aatcatgtca cgcaatttac aagttatgaa
    44641 agcagctgta acagaaggac taacctcaac caaatctatc agtggtttga cagggggcga
    44701 tgcggtcaaa atggataact acattaaaaa agggaacagc ctttctgata ctactattct
    44761 taatgctgtt agaaatgcta tagctgtaaa cgaattaaat gctaaaatgg gacttgtctg
    44821 tgcaacaccc actgcaggaa gtgcaggatg cttaccagca gtgctcgcaa cagctattga
    44881 aaaacttgac ttatcagaaa aaaaacaatt agagttttta tttactgcag gagcttttgg
    44941 tcttgttatt ggaaataacg cctctatttc tggtgcagaa ggtggatgcc aagcagaagt
    45001 tggttctgca gctgctatga gtgcagctgc tcttgttaaa gcagctggtg gaacatctca
    45061 tcaagctagt caagctattg cgtttgttat caaaaattta ttaggacttg tctgtgatcc
    45121 tgttgccggc ctagtagaag tgccttgtgt caaacgcaat gccctaggag caagtttcgc
    45181 ccttgtcgca gctgatatgg ctttagctga tattgaatct caaattcctg ttgacgaagt
    45241 tattgatgct atgtatcaag tcggctctgc catgccaact gctttccgtg aaacagcaga
    45301 aggtggttta gccgctactc caactggacg acgttacagc gtagaaatat ttggcgaata
    45361 aatagatagg tagaaaaatt cttagaaata tttgaaagcg ttttattttt ctgctatata
    45421 ttcactaaaa taactaatag atagctatca agctatgttg tttcccctta gaaacaactc
    45481 ctagtaaatt gttgaaactc aaaagtgtag tagtgtaaaa tactgaaaat tgctaatgac
    45541 cttataaaca tttaagagtt tttttcctgc ttgttatcag tataactgcc ttaaatatca
    45601 gaggcactta agtgctggtc agttttccta aataatacga gctatattcc ctgataaggc
    45661 aatgttttgt tttgagacta attcatcact acaaatcata aagacaatta aataccaaaa
    45721 acctattgtt agtaaatact aacaataggt tttatttctt ccagctatgt ttatttagta
    45781 accccaagct gataaacctt gtgcactata ggctttcaag gctgcattga cttgatcttc
    45841 aaccgttgct gtagaacccc aacctggcat tgtctggaac aaccctgatg ccccagaagc
    45901 attagttgcg ttaggatttc cattagattc acgcgcaatt atatgttccc aagttgattg
    45961 tggaacacct gttgctgctg ccatctgtgc cgccgcttga cttcctacaa taccagcagt
    46021 atttccattg gaaagtacca cattgtttgg attataggct actgaagata cctgctgaac
    46081 agtttgagtc acttcttgct gaacaaccgg tgcttcctct gttactgtcg gagctacaga
    46141 gttttctact gtgggaatag gctccgaagc aatgctcttt gctttttctt tgctttcttc
    46201 tttagtaata gcatgattat tttttggaac cttgtcgatt gttaccattt tactttctga
    46261 cttcttagca taagacttag tatcagcatt tttacttgag aaggcaaaaa tgatggctaa
    46321 tattgttaga gctaacgcta ctaatccaaa attaaaataa cgttgtttta aattttcttt
    46381 cttaaacatt caataatacc tcacttgtct ccgttatcca taatatcttt taattattac
    46441 ctcgttattg aagatatatt aaaaatatta caactacatt gatattataa gaagttgtaa
    46501 aaaatcataa aactcttctt cttagcaaca ttaaaaagcg ggataatatc attaaactaa
    46561 gaatttagtt ctgtattgaa caggactcag tccttttaga tttcgttcga tttgttattg
    46621 ttgggatgat taatatagtt gagatagcgg ttttcaattc ctcaaatgaa gaaaagtacc
    46681 tcaatcatta gatgttgtat ctaacttttg aggtacaatt cacttcttac taataaagaa
    46741 aagttttgtg ctaaaaaata ttacaaagag gttttcaaga aaaaaagtag caaacccaat
    46801 aaagaaacta ttccaatagc taaactatct tttagctgcc agtctaattg acgatactta
    46861 gttcgccctt ctcctccctg ataacctcta gcttccatag caatagctaa tgcgtcggct
    46921 cttttaaagc ttgaagcgaa aagagggatt aaaataggaa taatggattt aactttttga
    46981 attaaatttc cctctccaaa atcaactcca cgggcccttt gtgcattcat aatacgcgtg
    47041 gtatcatcca ttaaagtagg cacaaaacgt aaacttaaag aaagcattag tccgatctcg
    47101 tgagctggaa ctttaaatct tgttaaaggt tttaataatg attcaactgc atcagatagg
    47161 cttaaaggtg ttgtcgtcaa cgttaacagc gttgaaaaga aaataattag aacgaaacgc
    47221 ataaaaatta aaatagcttg gctcaatcct aaatcagtta tgctaatgaa ccaccaacta
    47281 aaaattactt ttcctccttg tgagaaaaac atctgaaata aagtcgtgaa gaggataata
    47341 ccaatcattg gtttaacccc atttaaaaag aaagacaact tgatttttga caaaaaaaca
    47401 actgctaaag taaaagtcaa catcaacaaa tttgtcacaa cattgttagc ccaaaagata
    47461 atgacaatat aaataatcat cgctaataat ttacttcttg ggtcaagacg atgaatgaga
    47521 gaatctccgg gaatataacg accaagaatc agcttatcca tgttttagta cctctcttag
    47581 ctcctctaag gtaataggca aagaagagat aggtattcct ctatcaacaa gcctttgagc
    47641 aagtttagtc accttaggaa ctcctaactg ttttttctca agcaaactga cttgttgaaa
    47701 aattgttttg ggttttcctg ataaaataat tttacctttg tcaagaacat atacaaaatc
    47761 tgcgtaattg gccacatcat ccatcagatg ggttactaaa acaatagtca tgccagattg
    47821 gtgtaacttt ttaaaaatag tcattaattc tttacgccct ttaggatcaa gaccagcagt
    47881 cggttcatct aaaactaaaa cctttggctg catagcaagg atacccgcaa tagcaacacg
    47941 acgcatttga ccccctgaga gttcaaatgg attcttttca aaaagatttt ctgaaatccc
    48001 aacaagagct agtttttcac gcgctaaagc ttctgcttcc tccggagaga ccccaaaatt
    48061 ttgagggcca aaagcaacat ctttcaaaac
//
LOCUS       DAUCLR010000002        42690 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.002, whole genome shotgun sequence.
ACCESSION   DAUCLR010000002 DAUCLR010000000
VERSION     DAUCLR010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 42690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42690
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.002"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            145..894
                     /gene="truA"
                     /locus_tag="VGT87_000058"
     CDS             145..894
                     /gene="truA"
                     /locus_tag="VGT87_000058"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEQ3812951.1"
                     /translation="MVRYKATISYDGTLFSGFQRQRHLRTVQEEIEKTLYKLNNGTKI
                     IIHGAGRTDAGVHAYGQVIHFDLPQEQEVEKLRFALDTQTPEDIDVVNIEKVADDFHC
                     RYQKHLKTYEFLVDNGRPKNPMMRHYTTHYPYTLNIKLMQEAINGLVGTHDFTGFTAA
                     GTSVQNKVRTITKATVSRDEKTDFLVFTFSGNGFLYKQVRNMVGTLLKIGNGQMPVEQ
                     VKVILSSKNRQLAGPTISGNGLYLKEICYEN"
     gene            884..1648
                     /locus_tag="VGT87_000059"
     CDS             884..1648
                     /locus_tag="VGT87_000059"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEQ3812952.1"
                     /translation="MKTDYIVTISGNDILSGGGLYADLATYIRYDLQAFVAVTCLTTR
                     SEEGFSLFPVAKEIFRDQLNSFTNAPISAIKIGLLPNAEMCEIVLDFIKGHVGIPVVL
                     DPVLACKEIDDVKIVPLRQEILQLLPYVTVVTPNLVEAQLLSQKEIVSLKDMQEAAKY
                     FYQLGAKQVVIKGGNRFSQKKAIDLFYDGKEIVTLECPVLEKNNIGAGCTFASSIASQ
                     LVKKKTPLEAVKNSKELVYQAILQSDRYGVKQSYAK"
     gene            1638..2108
                     /locus_tag="VGT87_000060"
     CDS             1638..2108
                     /locus_tag="VGT87_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEQ3812953.1"
                     /translation="MQNSKIRQMSLTGILTALVVVLGRFVMLPTPTGFLTLLDAGIYA
                     VSFSFGSAQGAIVGGLSGFLIDLVAGYPQWMFHSLIAHSVQGYFAGWRGRKRWFGVVI
                     GSFIMIFWYFLGSLMLGYGLSGSLAGIWGNVMQNTLGLFVGFIIFKAILRKKKR"
     gene            2122..2682
                     /locus_tag="VGT87_000061"
     CDS             2122..2682
                     /locus_tag="VGT87_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEQ3812954.1"
                     /translation="MLNNLEKQTREIVIDVVERSAIQPGNLFVLGLSSSEILGSRIGK
                     QSSLEVGQIVVEVVLDELNKRGVHLAVQGCEHVNRALVVERHVAESKQLEIVNVVPNL
                     HAGGSAQMAAFQLMSDPVEVEEVIAHAGLDIGDTAIGMHIKRVQIPLIPCQRELGGAH
                     VTALASRPKLIGGARADYNMDIIRKS"
     gene            complement(2747..3592)
                     /locus_tag="VGT87_000062"
     CDS             complement(2747..3592)
                     /locus_tag="VGT87_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEQ3812955.1"
                     /translation="MTFILHYLEQSHIENIGLTIFKKLVSLIILLLFFAILKRVTNYL
                     FEKTINKSFAYSRQSEARKKTLSKLTHNILNYLLYFLLIYWILSLFGIPVSSLLAGAG
                     IAGVAIGLGAQGFLSDVVNGFFILFENQFEVGDNVTISDIEGSVFGVGIRTTQIRGFD
                     GTLHFIPNRSITVVSNKSRGNMRALIEIPLYSTVNLSQVTRIIDEVNQKELPNHPQIV
                     GKPNILGPQNNSNGQFTFRIAIFTENGEQFKIYHTFYRLYQEALLKEGIQLPTAISYS
                     ITSKS"
     gene            3913..5196
                     /locus_tag="VGT87_000063"
     CDS             3913..5196
                     /locus_tag="VGT87_000063"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEQ3812956.1"
                     /translation="MSTSFENKATNRGVITFTISQDKIKPALDKAFNKIKKDLNAPGF
                     RKGHMPRPVFNQKFGEEVLYEDALNIVLPEAYEAAVTELGLDVVAQPKIDVVSMEKGK
                     EWTLSAEVVTKPEVKLGDYKNLVVEVDASKEVSDEDVDAKIERERQNLAELIIKDGEA
                     AQGDTVVIDFVGSVDGVEFDGGKGDNFSLELGSGQFIPGFEDQLVGAKAGDEVEVNVT
                     FPESYQAEDLAGKAAKFMTTIHEVKTKEVPELDDELAKDIDEDVDTLEDLKVKYRKEL
                     EAAQETAYDDAVEGAAIELAVANAEIVDLPEEMIHEEVNRSVNEFMGNMQRQGISPEM
                     YFQLTGTTQEDLHNQYSAEADKRVKTNLVIEAIAKAEGFEATDSEIEQEINDLATEYN
                     MPADQVRSLLSADMLKHDIAMKKAVEVITSTASVK"
     gene            5408..5983
                     /locus_tag="VGT87_000064"
     CDS             5408..5983
                     /locus_tag="VGT87_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEQ3812957.1"
                     /translation="MKLDVFAGQEKSELSMIEVARAILEERGRDNEMYFSDLVNEIQN
                     YLGKSDAGIRHALPFFYTDLNTDGSFIPLGENKWGLRSWYAIDEIDEEIITLEEDEDG
                     AQKRKKKRVNAFMDGDEDAIDYRDDDPEDEDFTEESAEVEYDEEDPDDEKSEVESYDS
                     ELNEIIPEDDFEEVDINEEDEEDEEDEEPVL"
     gene            6240..7844
                     /locus_tag="VGT87_000065"
     CDS             6240..7844
                     /locus_tag="VGT87_000065"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEQ3812958.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NIDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITDALKEKIKRAASTTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGSENVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPVE
                     QGIKNKLAQFCDVNSEAVIESRDVEHLYQIPLNLQAQSMDQIVCDHLKLNAPQADMTE
                     WSAMVDKVMNLRKTTKIALVGKYVELPDAYLSVVEALKHSGYANDTAIDLKWVNANDV
                     TVDNAADLLGDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGICLGMQLTCVEFA
                     RHVLNMEGANSFELEPSTKYPIIDIMRDQIDIEDMGGTLRLGLYPCKLKPGSKAAMAY
                     NNQEVVQRRHRHRYEFNNKFRPEFEAAGFVFSGVSPDNRLVEIVELKEKKFFVAAQYH
                     PELQSRPNRPEELYTAFVTAAIKNSN"
     gene            8012..8938
                     /locus_tag="VGT87_000066"
     CDS             8012..8938
                     /locus_tag="VGT87_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEQ3812959.1"
                     /translation="MKTIRIAKYLGILFLLITLISVGASFYFFHVAQIREEKSFINNK
                     KRSTNNPLYPAEQSFDALPYEKRQLTNRGLKQVGWYLPAAQKTKKTAIVVHGFTNDKE
                     DMKPYAMLFHDLGYNVLMPDNEAHGESEGNLIGYGWNDRLNVMAWTDQLIKENPESQI
                     TLFGLSMGAATVMMASGERLPAQVTSLIEDCGYTSVWDELKFQAKAMYNLPAFPLLYE
                     VSALSKIRAGFSYGEASSVKQLAKNKRPTLFIHGDKDDFVPTKMVYDNYKATKGPKEI
                     LIVKGAKHAKSFETNPEQYQKKIAAFLKKVEK"
     gene            8992..9077
                     /locus_tag="VGT87_000067"
     tRNA            8992..9077
                     /locus_tag="VGT87_000067"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9026..9028,aa:Leu,seq:aag)
     gene            9283..10164
                     /locus_tag="VGT87_000068"
     CDS             9283..10164
                     /locus_tag="VGT87_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEQ3812960.1"
                     /translation="MAIVSAEKFVQAARENGYAVGGFNTNNLEWTQAILRAAEAKQAP
                     VLIQTSMGAAKYMGGYKVCQSLITNLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLAKTAEVVKIAHAKGVSVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPENWEGLALDHLEKLTAAVPGFPIVLHGGSGIPDDQI
                     KEAIRLGVAKVNVNTESQIAFSNATREFARNYEANEAEYDGKKLFDPRKFLAPGMKAV
                     QGAVEERIDVFGSANKA"
     gene            10546..10734
                     /locus_tag="VGT87_000069"
     CDS             10546..10734
                     /locus_tag="VGT87_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEQ3812961.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRTVKPNLQKVTILVDGKP
                     KKVWASARALKSGKVERI"
     gene            10886..11251
                     /locus_tag="VGT87_000070"
     CDS             10886..11251
                     /locus_tag="VGT87_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEQ3812962.1"
                     /translation="MTVKINTKDGLIELSDDVIATVVGGSATEIFGVVGMASKSAIKD
                     NFQSLLRKENYAKGVVVKSTDLGISVDVYTVMSYGVKISEVSKNIQERVKFNLESQLG
                     LTADMVNVYVQNIKVVGEN"
     gene            11251..12915
                     /locus_tag="VGT87_000071"
     CDS             11251..12915
                     /locus_tag="VGT87_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEQ3812963.1"
                     /translation="MSNITTSLFQEMVQAAATRLGKQAEYVNSLNVFPVPDGDTGTNM
                     SMTMDNGAKEVADKPASTVGEVGQMLSKGLLMGARGNSGVITSQLFRGFGQSIKGKDE
                     LTGKDLAQAFQVGVEVAYKAVMKPVEGTILTVSRGAATAALKKADLTDDAVEVMQAAL
                     DGAKGALAKTPDLLPVLKEVGVVDSGGQGLVFIYEGFLSALNGDYVTSADFKATPANM
                     SEMINAEHHKSVVGHVATEDITYGYCTEIMVALKQGPTYVKEFNYDEFQGYLSGLGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLIKIKVDNMRNQHEAQVQKTDVEKNKA
                     EVKDFGLIAVVAGEGLSEIFKAQGVDYVISGGQTMNPSTEDIVKAIEAVNAKQVIILP
                     NNKNIFMAAQSAAEVVDIPAAVVATRTVPQGFTSLLAFDPSKSLEDNVADMSTSLSDV
                     VSGSVTLAVRDTTIDGLEIHENDFLGMVDGKIIVSNPDMEATLKAAFEKMIDEDSEIV
                     TIFVGEEGDQDLAEELAGYLGETYEDVEVEIHQGDQPVYPYLMSVE"
     gene            13152..14042
                     /locus_tag="VGT87_000072"
     CDS             13152..14042
                     /locus_tag="VGT87_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEQ3812964.1"
                     /translation="MLGPFIFIAFGVIVILAIVASTLYVVRQQSVAIVERFGRYQKTA
                     TSGIHVRLPFGIDKIAARVQLRLLQSEIIVETKTKDNVFVTLNIATQYRVNEQNVTDA
                     YYKLMKPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMSTYGYIIV
                     KTLITKVEPDAEVKQSMNEINAAQRKRVAAQELANADKIKIVTAAEAEAEKDRLHGVG
                     IAQQRKAIVDGLAESIQELKEANISLNEEQIMSILLTNQYLDTLNTFAAKGNQTLFLP
                     NTPSGVEDIRTQVLSALKTK"
     gene            14272..15126
                     /locus_tag="VGT87_000073"
     CDS             14272..15126
                     /locus_tag="VGT87_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HEQ3812965.1"
                     /translation="MKSKKVVSVISLTLSLFLVTGCAKVDNNKSVNRKPATKQTYNSY
                     SDDQLRSRENTMSVLWYQRAAETQALYLQGYQLATDRLKEQLNKPTDKPYSIVLDIDE
                     TVLDNSPYQAKNVLEGTGFTPESWDYWVQKKEAKPVAGAKDFLQFADQNGVQIYYISD
                     RSTTQVDATMENLQKEGIPVQGRDHLLFLEKGVKSKESRRQKVKETTNVTMLFGDNLL
                     DFADFSKKSQEDRTALLSDLQEEFGRRFIIFPNPMYGSWEGAIYKGEKLDVLKQLEER
                     RKSLKSFK"
     gene            15319..16515
                     /locus_tag="VGT87_000074"
     CDS             15319..16515
                     /locus_tag="VGT87_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEQ3812966.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKEGKSLAPVAADLAAKLGQDVVFPGVTRGSKLEE
                     AINALEDGQVLLVENTRFEDVDGKKESKNDEELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKDLLEKSNGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALAGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            16778..17329
                     /locus_tag="VGT87_000075"
     CDS             16778..17329
                     /locus_tag="VGT87_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEQ3812967.1"
                     /translation="MKTLRKLLSNYKFDIKKFKLGMRTLKTGLSVFLVLLVFHLFGWK
                     GLQIGALTAVFSLREDFDKSVHFGFSRIIGNSIGGLLSLVFFAFNEIFHQAFWVTLLI
                     VPICTMLCIMVNVACNNKTGIIGAVAALLIITLSIPTGQTFIYVTSRVFETFCGVFVA
                     ILVNTDVELIKNKWLNKKHSDVR"
     gene            17396..17767
                     /locus_tag="VGT87_000076"
     CDS             17396..17767
                     /locus_tag="VGT87_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEQ3812968.1"
                     /translation="MKEKELRRSMAVFPIGTVMTLTDLSARQIRYYEDQGLIKPERTQ
                     GNRRMFSLNDMDRLLEIKDFLSEGLNIAAIKREYVERQGKLMQKQKALTDADVRRILH
                     DEMLTQSGFSTPSQHIGNFRI"
     gene            17805..19151
                     /gene="glnA"
                     /locus_tag="VGT87_000077"
     CDS             17805..19151
                     /gene="glnA"
                     /locus_tag="VGT87_000077"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEQ3812969.1"
                     /translation="MAITVADIRHEVKEKNVTFLRLMFTDIMGVMKNVEIPATKEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWIVFPWGDENGAVAGLICDIYTAEG
                     KPFAGDPRGNLKRALKHMNEIGYKSFNLGPEPEFFLFKMDDKGNPTLEVNDNGGYFDL
                     APIDLADNTRREIVNILTKMGFEVEASHHEVAVGQHEIDFKYADVLKACDNIQIFKLV
                     VKTIAREHGLYATFMAKPKFGIAGSGMHCNMSLFDNQGNNAFYDEADKRGMQLSEDAY
                     YFLGGLMKHAYNYTAITNPTVNSYKRLVPGYEAPVYVAWAGSNRSPLIRVPASRGMGT
                     RLELRSVDPTANPYLALAVLLEAGLDGIINKIEAPEPVEANIYTMTMEERNEAGIIDL
                     PSTLHNALKALQKDDVVQKALGYHIYTNFLEAKRIEWSSYATFVSQWEIDHYIHNY"
     gene            complement(19379..21061)
                     /locus_tag="VGT87_000078"
     CDS             complement(19379..21061)
                     /locus_tag="VGT87_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEQ3812970.1"
                     /translation="MTNISLKPNEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKF
                     PEDDLLGIDYVIPDYSYIVDNLDRVKALVITHGHEDHIGGIPFLLKQANIPIYAGPLA
                     LALIRGKLEEHGLWREATVYEINHNTELTFKNMSVTFFKTTHSIPEPVGIVIHTPQGK
                     IICTGDFKFDFTPVGDPADLQRMAALGEEGVLCLLSDSTNAEIPTFTNSEKVVGQSIL
                     KIIEGIHGRIIFASFASNIYRLQQAAEAAVKTGRKIAVFGRSMEKAIVNGIELGYIKV
                     PKGTFIEPSELKNLHASEVLIMCTGSQGESMAALARIANGTHRQVTLQPGDTVIFSSS
                     PIPGNTTSVNKLINTIQEAGVDVIHGKVNNIHTSGHGGQQEQKLMLSLIKPKYFMPVH
                     GEYRMQKVHAGLAMDIGIPKENIFIMENGDILALTSDSARIAGHFNAQDIYVDGNGIG
                     DIGAAVLRDRRDLSEDGVVLAVATVDFNTQMILAGPDILSRGFIYMRESGDLIRESQR
                     VLFNAIRIALKNKDASIQSVNGAIVNALRPFLYEKTEREPIIIPMVLTPDKH"
     gene            complement(21063..21293)
                     /locus_tag="VGT87_000079"
     CDS             complement(21063..21293)
                     /locus_tag="VGT87_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEQ3812971.1"
                     /translation="MIYKVFYQETKDQSPRRESTKALYLNIDATDELDGRIKARRLVE
                     DNTYYNVEFIELLSDKHLDYEKETGVFELTEF"
     gene            21577..22275
                     /gene="tsaB"
                     /locus_tag="VGT87_000080"
     CDS             21577..22275
                     /gene="tsaB"
                     /locus_tag="VGT87_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEQ3812972.1"
                     /translation="MKTLAFDTSNKTLSLAILDDETLLADMTLNIQKNHSVSLMPAID
                     FLMTCTDLKPQDLERIVVAKGPGSYTGLRVAVATAKTLAYSLNIALVGISSLYALAAS
                     TCKQYPNTLVVPLIDARRQNAYVGYYRQGKSVMPQAHASLEVIIEQLVEEGQLIFVGE
                     TAPFAEQIQKKLPQAILLPTLPSAYECGLLGQSLAPENVDAFVPQYLKRVEAEENWLK
                     DNEIKDDSHYVKRI"
     gene            22247..22702
                     /gene="rimI"
                     /locus_tag="VGT87_000081"
     CDS             22247..22702
                     /gene="rimI"
                     /locus_tag="VGT87_000081"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEQ3812973.1"
                     /translation="MIVTTLSESNMKTVEEQAKNIYQLLEMVYGTSPWTLEQVLIDIR
                     RDQTDYFLLYDHDKLLGFLAIQDLAGEVEMTQIAILPSHQGLGLASQLMTHLDSIESD
                     IFLEVRESNHRAQGLYQKFGFKLIGKRPDYYRNPIETALLMKREGKHDR"
     gene            22692..23720
                     /gene="tsaD"
                     /locus_tag="VGT87_000082"
     CDS             22692..23720
                     /gene="tsaD"
                     /locus_tag="VGT87_000082"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEQ3812974.1"
                     /translation="MTDRYILAVESSCDETSVAILKNESTLLSNVIASQVESHKRFGG
                     VVPEVASRHHVEVITTCFEDALQEAGISASDLSAVAVTYGPGLVGALLVGLAAAKAFA
                     WANHLPLIPVNHMAGHLMAAREQKPLVYPLIALLVSGGHTELVYVPEPGDYHIIGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDQLAHKGQDTYHFPRAMITEDHLEFSFSGLKSA
                     FINLHHNAKQKGDELILEDLCASFQAAVLDILLAKTKKALSRYPAKMLVVAGGVAANQ
                     GLRDRLAQEITHIEVVIPKLRLCGDNAGMIALAAAIEYDKQHFANMSLNAKPSLAFDQ
                     FPDSFVIN"
     gene            complement(23891..24160)
                     /gene="rpsN"
                     /locus_tag="VGT87_000083"
     CDS             complement(23891..24160)
                     /gene="rpsN"
                     /locus_tag="VGT87_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEQ3812975.1"
                     /translation="MAKKSKIAKYQKQLQLIEQYADLRRDLKAKGDYESLRKLPRDSN
                     PNRLKNRDKIDGRPHAYMRKFGVSRINFRDLAHKGQLPGVTKASW"
     gene            complement(24395..25138)
                     /locus_tag="VGT87_000084"
     CDS             complement(24395..25138)
                     /locus_tag="VGT87_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ3812976.1"
                     /translation="MSTNDLTKKLKKLKHVQVYNTIFQLIQDGTYSPGMQLPSEPELA
                     RQLNVSRMTLRKSLALLQEDHLIKNIRGKGNFILKTPETKYHQGFEYLQHPIYASLSS
                     DITKVELEYRIEVPTVAITASLKQETPVVIIVDRWYHSQNKAIAYSLSCIPIEIISKY
                     AINLNQEEPLLTFLEEKIYESGKASHSCNQIGYTKTGNYTATKYTLSENSAFILIQET
                     LYNGKDILVSTKHYVPADLFDLKVQSQSC"
     gene            25378..26157
                     /gene="udp"
                     /locus_tag="VGT87_000085"
     CDS             25378..26157
                     /gene="udp"
                     /locus_tag="VGT87_000085"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HEQ3812977.1"
                     /translation="MQNYSGEVGLQYHLQIRPGDVGRYVIMPGDPKRCAKIAEHFDNA
                     VLVADSREYVTYTGTLNGEKVSVTSTGIGGPSASIAMEELKLCGADTFIRVGTCGGID
                     LDVKGGDIVIATGAIRMEGTSKEYAPIEFPAVADLEVTNALVNAAKKLGYTSHAGVVQ
                     CKDAFYGQHEPERMPVSYELLNKWEAWKRLGTKASEMESAALFVAASHLGVRCGSDFL
                     VVGNQERNALGMDNPMAHDTEAAIQVAVEALRTLIENDKSQ"
     gene            26178..27380
                     /locus_tag="VGT87_000086"
     CDS             26178..27380
                     /locus_tag="VGT87_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEQ3812978.1"
                     /translation="MQFIYSIIGILLVLGIVYAISFNRKSVSLSLIGKALIVQFIIAL
                     ILVRIPLGQQIVSVVSTGVTSVINCGQAGLNFVFGSLADSGAKTGFIFAIQTLGNIVF
                     LSALVSLLYYVGILGFVVKWIGKGVGKIMKSSEVESFVAVANMFLGQTDSPILVSKYL
                     GRMTDSEIMVVLVSGMGSMSVSILGGYIALGIPMEYLLIASTMVPIGSILIAKILLPQ
                     TEPVQKIDDIKMDNKGNNANVIDAIAEGASTGAQMAFSIGASLIAFVGLVSLINMMLS
                     GLGIRLEQIFSYVFAPFGFLMGFDHKNILLEGNLLGSKLILNEFVSFQQLGHLIKSLD
                     YRTALVATISLCGFANLSSLGICVSGIAVLCPEKRGTLARLVFRAMIGGIAVSMLSAF
                     IVGIVTLF"
     gene            27410..28081
                     /gene="deoC"
                     /locus_tag="VGT87_000087"
     CDS             27410..28081
                     /gene="deoC"
                     /locus_tag="VGT87_000087"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEQ3812979.1"
                     /translation="MEVKDILKTVDHTLLATTATWPEIQTILDDAMAYETASACIPAS
                     YVKKAAEYVSGKLAICTVIGFPNGYSTTAAKVFECQDAIKNGADEIDMVINLTDVKNG
                     DFDTVEEEIRQIKAACQDHILKVIVETCQLTKEELIELCGVVTRSGADFIKTSTGFST
                     AGATFEDVEVMAKYVGEGVKIKAAGGISSLEDAKTFIALGASRLGTSRIIKIVKNEAT
                     KPDSY"
     gene            complement(28176..29345)
                     /locus_tag="VGT87_000088"
     CDS             complement(28176..29345)
                     /locus_tag="VGT87_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEQ3812980.1"
                     /translation="MTQYDTIIIGGGPAGMMAAISSSYYSYKTLLIEKNRRLGKKLAG
                     TGGGRCNVTNSGNLDVLMAGIPGNGRFLYSVFSQFDNHDIIAFFEENGVKLKEEDHGR
                     MFPTTDKSRTIIDALEKKIKALGGQVLTSTEVVSVKKQDDLFYLKSADQTFTCQKLIV
                     TTGGKSYPSTGSTGFGHDIARHFKLTVTDLEAAESPLLTDFPHKVLQGISLDDVTLSY
                     DKHVITHDLLFTHFGLSGPAALRLSSFVKGGEIAELDFLPHLSTDDLTAYLSDQRDKN
                     IKNALKGLLPERVTDFLSEDYPEKVKQLSPKQEKELLDKLKHLQIPITGKMSLAKSFV
                     TKGGVDLKEINPKTLESKKVPGLYFAGEVLDINAHTGGFNITSALCSGWIAGKSS"
     gene            29606..29944
                     /locus_tag="VGT87_000089"
     CDS             29606..29944
                     /locus_tag="VGT87_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3812981.1"
                     /translation="MKKRFNSKAITLLAVSSLSLGFTAVTSVVSAATAHPAPSPIIDE
                     WNYGVSDNYGYSNYYVKSPNNIGSKSSITNYWGSIKASDSQKYGWALSSSKKAWNDIR
                     LNAHWNYFNF"
     gene            30012..31994
                     /locus_tag="VGT87_000090"
     CDS             30012..31994
                     /locus_tag="VGT87_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ3812982.1"
                     /translation="MKLKLSVIGLFFCLIAAIGVISISDTQKPVPLPIDGAFSIQGKS
                     SLSSDEVYKKISNLSKTKNVNIYKPIVRNSGQLTYINFKNTNYEQLKSAPITGMYYTL
                     GKIDSSSLKVLTASGLKLVYAAYPWYIGGILQFDGTLRLLLMLSIYLTLLVVLFVVRT
                     RQIKEGVIRHSLGLPIYNLRRDYVISFTFELVMAGLLMIAYSYFWGSGLFTYSSKLFF
                     SLILTNLIIFQIVDIITFILFWLTIRVEKPIEIIKNKAKNSFLFIVWLVIISIIIIVS
                     GIFLQETKASQSRINRQISQLSPWNKVRNWKRLELLGIENGSINNGEINEPESQYIQI
                     ATALKKLDFIYIKPSSVYIPDYMKNTNFAEDFFKKLNNDGITDPEVNKEMIYINKSGA
                     NIQNKVNGTDYQLLDNKVATIYIPDKFKEKKKSIENTVVAEQFTGTKYTKENLAVQII
                     PNEKKIFYFNENGNEHDENKEASPMANVTDSKGNIVVVLDTDKMVASKDFSLASNIVN
                     SSLFSPEAIKKINDLSLHLNFSINPVDVYQLVKLNIQSLKHQIFLSKVLQKIIYGIVF
                     LLIYQYAQLFISSKQNDYVKKIILGLSKTRIAISSLKYFIMTIAIVIICTFMVTEQIE
                     LLYIGIASLVVLILSTVMSFRKLSKKYTQILKGDEQ"
     gene            31991..32614
                     /locus_tag="VGT87_000091"
     CDS             31991..32614
                     /locus_tag="VGT87_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEQ3812983.1"
                     /translation="MTIEIFNVTKKFSSKKIFTDLNLTFEAGKSYALIGGSGSGKSTL
                     LNIIGRLEKIDNGKVLVDEQDIWKIKERTFFKNIIGYVFQNYSLIENKTVYDNLKLIN
                     KDKKKISAVLEKVGLSSDYLNQKIYELSGGQAQRVAIARMLMKPRKIILADEPTGALD
                     GEIGKEIINLLLSETAENKYVIIATHDPAVYNKVDFIIDMKDIGSKK"
     gene            32611..33102
                     /locus_tag="VGT87_000092"
     CDS             32611..33102
                     /locus_tag="VGT87_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3812984.1"
                     /translation="MKKKIYIGLIVIVGVFVVFLSGKQITQKETSNKPEVTLTVYTIS
                     SSDTQKWSKVKQFETKEAIYLVKVKETTSSEEVFSNIIADGMAVGFGVSEEDVKQFND
                     NLCSPVEDAKNNKLIGIEFINFSSVDKGFVVANFDYGKKAFNSQKTENKKLYKKLYEA
                     YKG"
     gene            33306..33836
                     /locus_tag="VGT87_000093"
     CDS             33306..33836
                     /locus_tag="VGT87_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEQ3812985.1"
                     /translation="MGIEKTVSELADILGVSRQAVNNRVKSLPEEDLDKNEKGVTVVK
                     RSGLVKLEEIYKKTIFDDEPISEETKQRELLEILVDEKNTEITRLYEQLKAKDAQLAS
                     KDEQMRVKDVQIAEKDKQLDQQQQLTAKAMADKETLKLELEEAKAEANQARLQVEEVQ
                     AEVGPKKGFLTRLFAK"
     gene            33885..34472
                     /locus_tag="VGT87_000094"
     CDS             33885..34472
                     /locus_tag="VGT87_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEQ3812986.1"
                     /translation="MKHLDTYIAFDLEFNTVNDVSHVIQVSAVKYDHHKEVDSFDTYV
                     YTDVPLQSFINGLTGITSDKIAAAPKVEEVMAAFKNFVGELPLIGYNAQKSDLPILAE
                     NGLDLRDQYQIDLFDEAYDRRSADLNGIANLRLQTVATFLGIKGRGHNSLEDARMTAA
                     IYKSFLETDTNKAYLSQQEEVTTDNPFAALGDFFD"
     gene            34625..35362
                     /locus_tag="VGT87_000095"
     CDS             34625..35362
                     /locus_tag="VGT87_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEQ3812987.1"
                     /translation="MSQFYSTGELANLAGVSIRTVQYYDQRGILIPTALTEGGRRLYT
                     DSDLEQLRMICFLRDLGFSIEQIRKVLAEENAAQVLELLLVDHIATAKEDLAAKEQQV
                     DIAVKILDRLRKQDPQSLDFLMDISLSMKNQKAWKKLQWKMGISIFLAIVSYVGLILL
                     SSYLQKFWLVYVAMGLFLPGFSWLVIYFRKQFIYLCPHCHQKFEPSFNEFALAGHTPR
                     TRKLTCSHCHQKSYCLELAKEDTVSRR"
     gene            complement(35419..35718)
                     /locus_tag="VGT87_000096"
     CDS             complement(35419..35718)
                     /locus_tag="VGT87_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEQ3812988.1"
                     /translation="MMNMQNMMKQAQKLQKQMEQKQADLAAMQFTGKSAQDLVTATFT
                     GDKKLVGIDFKEAVVDPEDVETLQDMTTQAINDALTQIDETTKKTLGAFAGKLPF"
     gene            complement(35911..36285)
                     /locus_tag="VGT87_000097"
     CDS             complement(35911..36285)
                     /locus_tag="VGT87_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ3812989.1"
                     /translation="MSAKKTFFASNLKYLRLKKNMEQLELANLLGRKSSSSISEWEKG
                     KYTPKSGLLSDIAAIFDVSLTALMEEDLTLENKGVSYQLPQRELAMISEQLSENNYTK
                     WVDFAQILLEQQAKEDRELDNH"
     gene            36455..36676
                     /locus_tag="VGT87_000098"
     CDS             36455..36676
                     /locus_tag="VGT87_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ3812990.1"
                     /translation="MTKSTLKELRTTKKMTQQELSHLTGISVRTIARYEKDTKKLRRA
                     KYEKLKGIAQALAVSVDDIFLGIDSEFMN"
     gene            complement(36756..39038)
                     /locus_tag="VGT87_000099"
     CDS             complement(36756..39038)
                     /locus_tag="VGT87_000099"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEQ3812991.1"
                     /translation="MRYNQFSYIPTSLERAAEELKELGFDLDLQKTAKANLESFLRKL
                     FFHYPDSDYPLSHLIAKNDMDALSFFQSEQELSKEVFDLLALQVLGFIPGVDFTEADA
                     FLDKLAFPIHFDETEIIKHIHHLLATRCKSGMTLIDDLVSQGMLTMDNDYHFFNGKSL
                     ATFDTSQLIREVVYVEAPLDTDQDGQLDLIKVNIIRPQSQKPLPTLMTPSPYHQGINE
                     VANDKKLYRMEKELVVKKRNKITVEDRDFIPLETQPCKLPIGQNLESFSYINSYSLND
                     YFLARGFANIYVSGVGTAGSTGFMTSGDYAQIESFKAVIDWLNGRATAYTSHSKTHQV
                     RADWANGLVCTTGKSYLGTMSTGLATTGVDGLAMIIAESAISSWYNYYRENGLVCSPG
                     GYPGEDLDVLTELTYSRNLLAGDYLRHNDRYQELLNQQSQALDRQSGDYNQFWHDRNY
                     LKNAHQIKCDVVYTHGLQDWNVKPRQVYEIVNALPSTINKHLFLHQGEHVYMHNWQSI
                     DFRESMNALLCQKLLGLANDFSLPEMIWQDNTCPQNWQERKVFGTSTIKELDLGQELL
                     LIDNHYGEDEFKAYGKDFRAFKAALFKGKANQALVDILLEEDLLINGEIVLQLKVKSS
                     ENKGLLSAQILDYGKKKRLGDLPIALTQSSIDNGQNFSREPLKELPFRENSYRVISKG
                     FMNLQNRNNLSSIETIPNNKWMTVRLPLQPTIYHLEKGDTLRVILYTTDFEHTVRDNS
                     NYALTIDLSQSQLIVPIASN"
     gene            complement(39060..39719)
                     /locus_tag="VGT87_000100"
     CDS             complement(39060..39719)
                     /locus_tag="VGT87_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEQ3812992.1"
                     /translation="MNHHILQRYIDNHNFPIIEKSYHKYLTFESLEEDFTYILKDGIV
                     KQSVLSKYGMEFNLRYVTGLEITSVLNTGYSKDMGEPYNVRIESEKASFYKVRRSAFL
                     KDINEDIELQGYVKDFYHNRLQKSMKKMQCMLTNGRIGAISTQIYDLMTLFGEELPNG
                     QILINFVITNEELGKFCGISTASSVSRILKQLKEKNIIRIDKQHIIITNLDKLKDNIV
                     F"
     gene            39885..41081
                     /locus_tag="VGT87_000101"
     CDS             39885..41081
                     /locus_tag="VGT87_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ3812993.1"
                     /translation="MEEKLFNKHFVAITVINFIVYMVYYLFTVIIAFVATRELGAQTS
                     QAGLATGIYILGTLLARLIFGKQLEVFGRRLVLRGGAIFYLLTTLAYFYMPTISMMYL
                     VRFLNGFGYGVVSTATNTIVTAYIPACKRGEGINFYGLSTSLAAAIGPFVGTFMLDNL
                     HIDFRMIIVLCSVLIGCVVVGAFAFPVKNMSLNAEQLAKTKSWTVDSFIEKKALFITA
                     IAFLMGIAYASVLGFQKLYTSEIHLTAVGAYFFVVYALIITITRPAMGRLMDAKGDKW
                     VLYPSYLFLAMGLFLLGSVSSGGSYLLSGALIGFGYGTFMSCGQAASIQGVDEHRFNT
                     AMSTYMIGLDLGLGAGPYLLGLIKDLALGSGVASFRHLFWLAAVIPLICTLLYLLKTK
                     TRQVVS"
     gene            41553..42401
                     /locus_tag="VGT87_000102"
     CDS             41553..42401
                     /locus_tag="VGT87_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEQ3812994.1"
                     /translation="MEMTWTVKYITEFIATAFLIILGNGAVANVDLKGTKGHNSGWLV
                     IAFGYGLGVMMPALMFGNVSGNHINPAFTVGLAVSGLFPWAHVLQYVVAQLLGAIFGQ
                     LVVVMVYKPYFMKTENPNHVLGSFSTISSLDNGQKDSHKVSYINGFLNEFVGSFVLFF
                     GALALTKNYFGVELVGKLIEAGYDQTTAATQISPYVTGSLAVAHIGIGFLVMVLVTSL
                     GGPTGPALNPARDFGPRLLHHFLPKSVLGQAKGDSKWWYAWVPVVAPILAAIVAVAAF
                     KYLYIR"
BASE COUNT        13602 a         6941 c         8797 g        13350 t
ORIGIN      
        1 agagagagtt tggaggaaat agctttctta tagcttttaa atcgttagta gcttagagta
       61 agcttcaata cgtctttcat ttgtttgggt acagtttgat taaaaatagt ttctcttacg
      121 tttaagaaat gttaaaatag taacatggta agatacaaag caacgatttc ctatgatggg
      181 actctttttt cgggttttca aagacaaaga catttacgta cagttcaaga agagattgaa
      241 aaaacacttt ataaattaaa taacggtaca aaaattataa ttcatggtgc aggacgtacg
      301 gatgcaggag tacatgctta tggacaggtg atccattttg atttgcctca agaacaagaa
      361 gttgaaaaat tacgatttgc tcttgataca cagactcctg aagatattga tgttgttaat
      421 atagaaaaag tggctgatga ttttcactgt cgttatcaga agcatttaaa aacttatgaa
      481 tttttagttg ataatggtcg acctaaaaat ccgatgatgc gacactatac cactcattac
      541 ccttatactc ttaatattaa acttatgcaa gaagctatta acggtctggt aggtacacac
      601 gattttactg gttttacagc agcaggaact tcggtacaaa ataaggtgcg aacgattacc
      661 aaagcaacgg tatctcggga tgaaaaaact gattttttgg tgtttacttt ttcaggaaat
      721 ggttttctct ataagcaggt gaggaatatg gtgggaactt tgctgaaaat tggtaatggt
      781 caaatgccag ttgaacaagt taaagtgatt ttgagctcca aaaatcgtca gctagctgga
      841 ccaacgatat ctggaaatgg gctttacttg aaggagattt gttatgaaaa ctgattatat
      901 cgtgactata tctgggaacg atattttaag tggaggtggc ctctatgctg atttagcgac
      961 ttacataagg tatgacctac aggcctttgt agcggtaact tgtttaacta ctagatctga
     1021 agagggcttt agtctatttc cggtagctaa agaaattttc cgtgatcagt tgaacagttt
     1081 tacgaacgct ccaatttctg ctattaaaat tggtttgtta cctaatgcgg aaatgtgtga
     1141 aatagttttg gattttataa aggggcatgt gggaatccca gtggttttag atcctgttct
     1201 agcatgtaaa gaaatagatg atgttaaaat agttcctcta agacaggaga ttttacaatt
     1261 attgccttat gtaactgttg ttactccaaa tttggtagag gctcagctat tatctcaaaa
     1321 agagattgtt tctttaaaag atatgcaaga agctgctaaa tatttttatc aacttggggc
     1381 aaaacaggtg gttatcaagg gtggtaatcg atttagtcag aaaaaggcta ttgatctttt
     1441 ttatgacggt aaagagattg ttaccttgga atgtcctgta cttgagaaaa ataatattgg
     1501 cgcaggctgt acttttgcat ctagtatcgc tagtcagtta gtaaagaaaa aaacgccact
     1561 agaagctgtg aaaaactcaa aagagttagt ctaccaagct attttacaat ctgatagata
     1621 cggagtaaaa caaagttatg caaaatagta agatacgaca aatgagttta acagggatat
     1681 tgacagcatt ggttgttgtt ttaggaagat ttgtcatgct acctactcca acaggttttt
     1741 taacgctgtt ggatgcagga atttatgctg tcagcttttc ctttggatca gctcaaggag
     1801 ctattgtggg agggttatca ggttttttaa tcgatttagt ggcaggatac cctcagtgga
     1861 tgtttcatag tttgattgct cacagtgtcc aagggtattt tgcgggatgg agaggacgca
     1921 agcgttggtt tggtgttgtg ataggttctt ttatcatgat tttttggtat tttttaggct
     1981 ctcttatgct tggttatggc ctttcgggat ctttagccgg tatttgggga aacgtcatgc
     2041 aaaatacatt aggacttttt gttggtttta ttatctttaa ggccatatta aggaaaaaaa
     2101 agagataagg agagtgagaa gttgttgaat aatttagaga aacaaacaag ggaaatcgtt
     2161 attgatgttg tagaacgctc tgctattcaa ccaggtaatc tttttgttct aggtctttca
     2221 tctagcgaaa ttttgggtag tagaattgga aaacaatcta gtctagaagt aggtcaaata
     2281 gttgttgaag ttgtgttaga tgagttaaat aagcgtggtg ttcatttggc tgtacaaggt
     2341 tgtgaacacg taaatcgtgc cttggtagta gagaggcatg tggcagaatc taagcagtta
     2401 gagattgtca atgtggttcc gaatcttcat gctggtggca gtgctcagat ggctgctttt
     2461 caacttatgt cagaccctgt cgaagtagaa gaagttattg cacacgctgg acttgatata
     2521 ggtgatactg ctataggaat gcatattaag cgtgttcaga tccctctgat tccttgtcaa
     2581 cgagagctag gaggtgctca tgtgacagct ttggctagtc gaccaaaatt aattggtgga
     2641 gctagggctg attacaatat ggatattatc cgtaaatcct aaaccctttt ttctggaata
     2701 aaacaattgg tgttttttct agaaaaaagg attttttaag attatattaa gattttgagg
     2761 tgatagaata gctgattgca gtcggtaatt gaatgccttc tttgagtaaa gcttcttgat
     2821 aaagtctata aaaagtatgg taaatcttaa attgttctcc attttctgtg aaaatagcaa
     2881 ttctaaaagt gaactgtcca ttagagttat tttgaggccc taaaatattt ggtttgccaa
     2941 cgatttgagg atggtttggc aattcctttt gattcacttc atcaatgatt cgagtgactt
     3001 ggctcaaatt aacagtggaa taaagaggaa tttcaattaa ggcacgcata ttaccacgag
     3061 atttgttgct gacaacggtg atgctacggt ttgggatgaa atgtagtgtt ccatcgaatc
     3121 cacgaatttg agtagttcta attccaacac caaagacact gccttctatg tcagagatag
     3181 taacgttatc tcctacttca aattgattct caaataagat aaaaaagcca ttgacaacgt
     3241 cagataaaaa tccttgtgca cctaacccga ttgctactcc agcaattcca gctcctgcta
     3301 gaagactaga gacaggtatg ccaaataagc taagtatcca ataaatgaga agaaaatata
     3361 ataagtagtt taaaatgttg tgagtgagct tagagagtgt ttttttgcga gcttcacttt
     3421 gtcttgaata agcaaacgat ttgttaatgg ttttttcaaa aagatagtta gtaacacgtt
     3481 ttaaaatagc aaaaaagagt aatagaataa tgagagacac tagcttttta aaaatcgtta
     3541 agccaatatt ttcgatatga gactgctcta ggtaatgtaa tataaatgtc atgagaaatc
     3601 ctttctttga tagtttatca aaaaaaaacc aatcttgaaa agagataaat taatatttta
     3661 accaaagtta tgataattgt tttatcataa agaatgccat agtcatccgt tgtgataatg
     3721 cttgaaaata gtcagttagt aagcgaaaaa gtctaacatt aacctttata aattgaaaat
     3781 aactgttagt agactaatga caggtagata ctcttaatag cgcgtttgat ttcacgcata
     3841 aaaagcttta aaaacgcttt aaattatgat aaaatgaact gtatcgaaat tattttaagg
     3901 agaaatgact taatgtctac atcatttgaa aacaaagcta caaatcgtgg cgtgattaca
     3961 tttacaatca gtcaagataa aataaaacca gctcttgata aagcttttaa taaaatcaaa
     4021 aaagacttaa atgcaccagg tttccgtaaa ggacacatgc ctcgtccagt cttcaaccaa
     4081 aaatttggtg aagaagttct ttatgaagat gctttgaata ttgtattgcc agaagcttat
     4141 gaagcagctg tgacggaact tggtcttgat gtggttgcac aaccaaaaat cgacgttgtg
     4201 tcaatggaaa aagggaaaga gtggacactt tctgctgaag ttgtgacaaa acctgaagtg
     4261 aaacttggtg attacaaaaa ccttgttgta gaagttgatg cttcaaaaga agtttcagat
     4321 gaagacgtgg atgctaaaat tgagcgcgaa cgtcaaaacc ttgcggaact cattattaaa
     4381 gacggtgaag cagctcaagg tgacactgtt gtgattgact ttgttggttc agttgatggt
     4441 gttgagtttg atggcggtaa aggagataac ttctctcttg aacttggttc aggacaattt
     4501 atcccaggtt ttgaagatca actagttggt gctaaagctg gcgatgaagt agaagttaat
     4561 gtcacattcc cagaatctta ccaagcagaa gatcttgcag gtaaagccgc taaatttatg
     4621 acaactattc acgaagtcaa aacaaaagaa gtacccgagc ttgatgatga gcttgcaaaa
     4681 gatattgatg aagatgttga cacacttgaa gacttaaaag taaaatatcg taaagaactt
     4741 gaagcagctc aagaaactgc ttatgatgat gctgttgaag gagctgcgat tgaattagca
     4801 gttgcaaatg ctgaaattgt tgatttaccc gaagaaatga ttcatgaaga agtcaaccgt
     4861 tcagtgaatg aatttatggg caacatgcaa cgtcaaggaa tctcacctga aatgtacttc
     4921 caattgactg gtacaactca agaagattta cataaccaat attcagctga agctgacaaa
     4981 cgtgttaaaa caaaccttgt tattgaagca attgctaaag cagaaggttt tgaagcaaca
     5041 gatagtgaaa ttgaacaaga aattaatgac cttgcaacag aatataacat gccagctgac
     5101 caagttcgtt ctcttctttc agcagatatg ttgaaacatg atattgcaat gaaaaaagca
     5161 gttgaagtga ttacaagcac agcaagcgtt aagtaatctt attaaggaac aaaacgtaca
     5221 agccggatta gaacgtcttt ctagtctggt ttttcttttt ctaaagataa ggaattagtt
     5281 aaacgttatt ttaatgaatt agctttaaag atttgtcaag aagaagtgat atgagttgat
     5341 aaaagttttg acgaattact ttttttagcg taaaatggta gagaatgata aaataaggag
     5401 aactgccttg aaattagacg tatttgcagg acaagaaaaa agcgagcttt ccatgattga
     5461 agtggcgcgt gccattttgg aagaacgcgg tcgcgacaat gagatgtact ttagcgattt
     5521 agtcaatgag attcaaaatt acttaggaaa atctgatgca ggtattcgtc atgcattacc
     5581 atttttctat actgacttaa atacggatgg ctcatttatt cctctaggtg aaaacaaatg
     5641 gggacttcgt tcgtggtatg ctatcgatga aattgatgaa gaaattatta ctctagaaga
     5701 agatgaagat ggcgcacaaa aacgtaagaa aaaacgcgtt aatgctttca tggatggaga
     5761 tgaagacgct attgattata gagatgatga tccagaagat gaagatttca cagaagaatc
     5821 agctgaggtt gaatatgatg aagaagatcc agatgatgaa aaatcagaag tggagtcata
     5881 tgattcagaa ttgaatgaaa ttatccctga agatgatttt gaagaagttg atatcaatga
     5941 agaagacgaa gaagatgaag aagatgaaga accagttctc taatgtcttt agttcagaaa
     6001 taaaaatgta attatcattt gacaaattgt ctattatagt ttatattatt attcgggcac
     6061 ctctttaaga ggtcaggcaa aagctctcta gttaataggg ggctattttt gttttctaga
     6121 ataatgccag tttgcctcta tatttagaaa actaaggtga tacctaacca atgtggaacc
     6181 aactaatagg gaattaacaa gaaagcaatg cttttattca gcttatataa aggagtacta
     6241 tgacgaaata catttttgta actggtggtg ttgtatcatc aattggcaaa ggaattgttg
     6301 cggcaagtct tggccgattg ttaaaaaatc gtggacttaa ggtgacgatt caaaaatttg
     6361 atccttatat taatattgat ccagggacga tgagccccta tcagcatgga gaagtatatg
     6421 tgacagatga cggtgctgaa accgacttgg atttgggtca ctatgaacgt tttattgata
     6481 tcaatcttaa taaatattcc aatgttacaa caggtaaaat ttatagtgaa gtccttcgta
     6541 aagaacgtaa aggtgagtac cttggagcga ctgttcaggt gattcctcat atcacagatg
     6601 cccttaaaga aaaaatcaaa cgcgcagctt caacaacgga ctctgatgta attattactg
     6661 aggtcggggg gactgttggc gatattgaaa gtttaccgtt cttagaagct cttcgtcaga
     6721 tgaaagctga tgttggttct gaaaatgtga tgtatattca caccaccttg ttgccatatt
     6781 taaaggcagc gggtgaaatg aaaactaagc caacgcaaca ttccgttaaa gaattgcgtg
     6841 gtttagggat tcaaccgaat atgttggtta ttcgtacgga agagccagtt gagcagggga
     6901 ttaaaaataa attggctcag ttctgtgatg tgaattcaga agctgttatc gagtcacgtg
     6961 atgtagaaca cttgtatcaa attccattga acttgcaagc tcaatcaatg gatcagattg
     7021 tttgtgatca tttgaaattg aatgcgccac aagctgatat gaccgagtgg tcagctatgg
     7081 ttgacaaggt gatgaattta agaaaaacaa caaaaattgc cttggttggt aaatatgtgg
     7141 aattgccaga tgcctactta tctgttgtgg aagccttgaa acattctggt tatgccaatg
     7201 ataccgcaat tgacctaaaa tgggtcaatg ctaacgatgt gacggtggat aatgcagctg
     7261 acttgttggg agatgcggat gggattattg taccaggtgg ttttggtcaa cgtggtacag
     7321 aaggcaagat ccaagcgatt cgctatgccc gcgaaaatga tgtgccaatg cttggtatct
     7381 gtcttggcat gcagttgact tgtgttgaat ttgcccgcca tgtcctcaat atggaaggtg
     7441 caaactcttt tgaattagag ccaagtacta aataccccat cattgatatc atgcgtgatc
     7501 aaattgatat tgaagatatg ggaggtacgc ttcgtctggg attgtaccca tgtaaattga
     7561 aaccaggttc aaaagcagca atggcttaca ataatcaaga ggtggtgcaa cgtcgtcatc
     7621 gtcaccgtta tgagttcaac aacaaattcc gtccagaatt tgaagcagct ggttttgttt
     7681 tctcaggggt atcacctgac aatcgcttag ttgaaattgt agaattgaaa gagaaaaaat
     7741 tctttgtagc agctcaatac cacccagaat tgcaaagccg tccaaatcgt cctgaggagc
     7801 tatacaccgc ttttgttaca gctgctatta aaaatagcaa ttagtggttt taagggtaag
     7861 tttgtggcat tctacttggc tggcagatac tgataaagat tactagctat tagagggatc
     7921 ttttctataa aaatagcatt agaaaaagaa aaacgtgata tttttaggtt agcactttct
     7981 ttttcagtca tttttttgta aaatacttgg tatgaaaact attcgaatag caaagtattt
     8041 aggaattcta ttccttttga taactcttat tagcgtaggt gctagttttt atttctttca
     8101 tgttgcacaa ataagagaag agaaatcgtt tattaataat aagaaacgga gtacgaataa
     8161 tccattatac ccagctgaac agtcttttga cgctttacct tacgaaaaac gtcaactaac
     8221 aaatcgtggg ttaaaacaag tggggtggta cttaccagct gctcaaaaaa caaaaaagac
     8281 agctattgtt gttcatggtt ttacgaatga caaagaagat atgaagccat atgccatgct
     8341 ttttcatgat ttgggctata atgtcttaat gccagacaat gaggcccatg gggaaagtga
     8401 agggaacttg attggttatg gctggaatga ccgccttaat gtcatggctt ggacagacca
     8461 actgattaag gaaaaccctg aaagccaaat cacactcttt ggcttatcta tgggtgctgc
     8521 aacagtaatg atggcaagtg gtgagcgatt gcctgcgcaa gtcacctccc tcatcgaaga
     8581 ttgcggttat accagtgttt gggacgaatt gaagtttcag gccaaggcta tgtacaactt
     8641 gcctgccttt cctttactct atgaagtctc tgccttatct aagattcgag caggttttag
     8701 ttacggagaa gcgagctcag tgaaacagct ggctaaaaat aaacgtccaa ctttatttat
     8761 ccacggtgat aaggatgatt ttgttcctac aaaaatggtt tatgacaatt ataaggccac
     8821 gaaaggtcct aaggaaatct tgattgttaa aggggcaaaa cacgccaaat cctttgaaac
     8881 aaacccagaa caataccaga aaaaaattgc cgcttttttg aaaaaagttg agaaataagt
     8941 attgacagtt aaactcacag ttgctataat aatttatgtt actgatgagt agcggggatg
     9001 gcggaattgg cagacgcgca ggactaagga tcctgtgacc gctttaggtc gtgtgggttc
     9061 aagtcccact ctccgcataa tgtaagaggt gaatcattga ttcgtctctt tttttggtta
     9121 aatgaaggat aagaaggcat agatttactg gagaaaactt ttgataatct gcttttggaa
     9181 aacgctttaa gcttgtgaaa gactagaaat ttatggaaaa ttgtgctaga atgaacaatg
     9241 taaacgggga gttatcccga aaaataagag gaggccttac aaatggcaat cgtttcagca
     9301 gaaaaatttg tccaagcagc tcgtgaaaac ggatatgctg ttggtggatt taacacaaac
     9361 aacttagagt ggactcaagc aatcttgcgt gcagcagaag ctaaacaagc tccagttctt
     9421 atccaaacat caatgggtgc agcaaaatac atgggtggtt ataaagtatg tcaatctctt
     9481 attactaacc ttgttgagtc aatgggtatc actgttcctg ttgctatcca ccttgatcat
     9541 ggtcactacg aagatgctct tgagtgtatc gaagtaggtt acacttcaat catgtttgac
     9601 ggttcacacc ttccagttga agaaaacctt gctaaaactg cagaagttgt taaaattgca
     9661 catgctaaag gcgtttcagt tgaagctgaa gttggtacaa tcggtggtga agaagacggt
     9721 atcatcggta aaggtgagct cgctccaatc gaagatgcta aagcaatggt tgaaactgga
     9781 attgacttct tggcagctgg tatcggtaac atccacggtc catacccaga aaactgggaa
     9841 ggtcttgctc ttgaccactt agaaaaatta acagcagctg taccaggttt cccaatcgta
     9901 ttgcacggtg gttctggtat tcctgacgat caaatcaaag aagcaatccg tcttggtgtt
     9961 gctaaagtta acgttaacac tgaaagtcaa atcgctttct ctaacgcaac tcgtgaattt
    10021 gctcgtaact acgaagctaa cgaagcagaa tatgatggta aaaaattatt tgacccacgt
    10081 aaattcttgg ctccaggtat gaaagctgtt caaggtgctg ttgaagaacg tatcgatgtt
    10141 ttcggatcag ctaacaaagc ataatttaga gtattaaata ccctaaagcc ctcttgaggg
    10201 cttttttaga tacaattgac accctaaaat ggataccagt ttctgtgaaa caatctttga
    10261 ttgtgaacaa acaaagcata atttagagta ttaaaatctg gaccctgcta tctatggtaa
    10321 gtctttttgt tattgttaaa gaggttaagc ggaatgttaa tatgttttag ctaaaaaatg
    10381 tagtgaaaaa tgagtacgta gactattgta atagtaattc cgtaaaattg ttgaaaaaga
    10441 aaatgggaaa taccttgtca aattaagcac cctatgatag aatagatagg tatgtggtgc
    10501 tagcacatcc gtaaacatat tctacgtagg aggaaacaga tcaaaatggc taaagtatgt
    10561 tattttacag gacgtaaaac cgtttctgga aacaaccgtt cacacgcgat gaaccaaacg
    10621 aaacgtacag ttaaaccaaa ccttcaaaaa gttactatcc ttgttgatgg taaacctaaa
    10681 aaagtttggg cttctgctcg tgcgcttaaa tctggtaaag tagaacgcat ctaatagaaa
    10741 taagccttag ggcttttttt attagcttat ttctatccat aagctagtca gggtaataaa
    10801 cgctattgtt aactattcta tctcttttac attacttcta aaatatgata aaataggatg
    10861 ataaaaactt tttgaggtag taactatgac tgtaaaaatt aatacaaaag atggtctaat
    10921 cgagctatcc gacgatgtga ttgcaactgt cgtgggagga tcagcaacgg aaatttttgg
    10981 tgttgttgga atggccagca aaagtgcaat aaaagacaat tttcaatcac tacttcgcaa
    11041 agaaaactac gctaagggtg ttgtagtaaa atcaacagat ctgggtattt ctgttgatgt
    11101 ctacactgtg atgagttatg gtgtgaaaat cagtgaagtc tctaaaaata tccaagaacg
    11161 cgtgaaattt aaccttgaaa gtcagcttgg tctcactgct gatatggtca atgtttacgt
    11221 acaaaatatt aaggttgtag gagaaaatta gtgtcaaata ttacaacaag cttattccaa
    11281 gaaatggtcc aagcggcagc gactcgcctt ggcaaacaag cagaatatgt taattcctta
    11341 aatgtttttc ctgttccaga tggtgataca ggaacaaaca tgagcatgac tatggacaat
    11401 ggtgccaagg aagttgctga taaacctgcg tcaactgtgg gagaagttgg acaaatgtta
    11461 tcaaaaggtc ttttgatggg agcgcgtgga aattctggag ttattacttc acaacttttt
    11521 cgtggttttg gtcaaagcat taaaggaaaa gatgagctga caggtaaaga tttggcacaa
    11581 gcctttcaag tgggtgtaga agttgcctac aaagcagtga tgaaacctgt tgaaggaaca
    11641 attttaactg tttctcgggg tgcagcgact gctgccttaa aaaaagcaga tcttacagac
    11701 gacgccgtgg aagtcatgca agctgcttta gacggtgcca aaggtgccct tgctaagaca
    11761 cctgatttat taccagtcct taaagaagtt ggtgtggttg attcaggcgg tcaaggtctt
    11821 gtctttatct atgaaggttt cctatctgct ttgaatggcg actatgtgac ttcagcagac
    11881 tttaaagcga ctcctgctaa catgtctgaa atgattaatg ctgagcatca caaatccgtt
    11941 gttggtcatg tggctactga agacattacc tacggttatt gtactgaaat catggttgcc
    12001 cttaaacaag gcccaaccta tgtgaaagaa ttcaactatg atgagtttca aggctacctt
    12061 agtggccttg gtgactcgct gttagtggtc aatgatgatg agattgttaa agttcacgtc
    12121 catactgaag accccggact tgtgatgcaa gaaggtctta aatatggttc gctcatcaag
    12181 attaaagtgg acaatatgcg taaccagcat gaagcacagg ttcaaaagac tgatgtcgaa
    12241 aaaaacaagg ctgaagtaaa agactttggc ttgattgcag tagtagctgg agaaggttta
    12301 tctgaaattt tcaaggcgca aggtgttgat tacgtgattt ctggtggtca aaccatgaat
    12361 ccatctacag aagatattgt taaagctatt gaagcagtta atgccaaaca agtcattatc
    12421 ttgccaaaca ataaaaatat ctttatggct gcccaatctg ctgcagaagt ggtggatatt
    12481 ccggctgctg ttgttgcaac acgtactgtt ccgcaaggat ttaccagctt gttggccttt
    12541 gatccttcaa aatctttaga ggacaatgta gcagatatgt caactagtct ttctgacgtg
    12601 gtcagtggaa gtgttacttt ggcagtccgt gacaccacca ttgatggttt agaaatccat
    12661 gaaaatgatt tcttgggaat ggttgatggt aaaatcatcg tttcaaaccc agacatggaa
    12721 gcaactctaa aagctgcctt tgaaaagatg attgacgaag atagcgagat tgtgactatc
    12781 tttgttggtg aagaaggtga ccaagactta gcagaagaac ttgctgggta tttaggagag
    12841 acttacgaag atgtggaagt tgagatccat caaggagatc agcctgttta cccatatctc
    12901 atgagtgtag aataatagaa tacaataaaa aaagaggagt taggcaaagg tctgactctt
    12961 tttgtctata ttgatgtcgt aatgagtggt tttagtgatg gtaagcccct aaaaaatgag
    13021 ggtaatccat cacaaagcta atggttacga tttaatttga caaaacctag aaaaacatta
    13081 taattgtatt ataacaataa gtcaaggact gaaaacggtt cagttctaag acattacaag
    13141 gaggagattt tatgttagga ccatttatct ttattgcttt tggggttatt gtaatcctag
    13201 ctattgttgc cagtaccctt tatgttgttc gtcaacaatc tgttgccatc gtggaacgtt
    13261 ttggtaggta ccaaaaaaca gccactagtg gtattcatgt ccgccttccg tttgggattg
    13321 acaaaattgc tgcgcgtgtg cagctgcgtc ttttacagtc agaaatcatc gttgaaacca
    13381 aaaccaagga caatgtgttt gtaactttga atattgccac tcagtatcgt gttaacgaac
    13441 aaaatgtaac agatgcttac tacaaattaa tgaaacctga atctcaaatc aagtcttata
    13501 ttgaagatgc cttgcggtca tccgtgccga agttgacctt agacgaattg tttgagaaaa
    13561 aagatgaaat tgccttggaa gtacaacatc aagtggcaga agaaatgtca acctatggat
    13621 atattattgt caaaaccttg attaccaagg tggagcctga tgctgaagtc aaacagtcaa
    13681 tgaatgaaat caatgcggct caacgcaaac gtgtagctgc tcaagagttg gcaaacgcag
    13741 ataaaattaa aattgtgaca gcagcagaag cagaggctga aaaagatcga ttgcacgggg
    13801 tagggattgc ccaacagcgt aaggccattg tggatggttt ggcggagtct attcaagagc
    13861 ttaaagaagc gaatatttcc ttaaacgaag aacaaatcat gtccattttg ttgaccaacc
    13921 aatacctgga taccctcaat acctttgcgg caaaaggaaa ccaaaccctt tttctaccaa
    13981 atacaccaag tggggtagag gatatccgta cacaagtttt atctgccctt aaaacaaaat
    14041 aacgtgtaaa agcttggcta aaaagttgct caatactgga tttagcagaa ggctatcata
    14101 ccttgtgaag tggcaaacga cgaaatgaat acattacttt tattggagaa atgaaagtaa
    14161 aaaaagcctt tttcatgatt aacaatatag tattgattaa atgttaagtt gtattgactt
    14221 ttaaattaaa taagtttaaa atcaacttaa taagcaaaaa ggagattttc aatgaaatcc
    14281 aaaaaagttg ttagtgttat atcacttacc ttatcccttt ttttggtgac aggttgtgct
    14341 aaagttgata acaacaaatc agttaatcgt aagcctgcta ctaaacaaac ctataatagc
    14401 tatagtgatg accaattaag atcgcgtgaa aataccatgt ctgttttatg gtaccagcga
    14461 gctgcggaaa ctcaagcgct ttatttacag gggtatcagt tagcaacgga tcgcttaaaa
    14521 gaacaactca ataaaccaac ggataaacct tattcaattg tattagatat tgacgaaaca
    14581 gttcttgata atagccctta tcaagctaaa aatgttttgg aaggaacagg atttacacct
    14641 gaaagctggg attattgggt acaaaagaaa gaagcaaaac cggttgctgg tgctaaagac
    14701 tttttgcaat ttgcagacca aaatggtgtt caaatttact acatttcaga cagatcaact
    14761 actcaagtag atgctacaat ggaaaatctc caaaaagaag gtattccagt acaaggtcgt
    14821 gatcatcttc tattcttaga aaaaggagta aaatcaaagg agagtcgtcg tcaaaaggtc
    14881 aaagaaacaa ctaatgtaac gatgctattt ggtgataatc ttctagattt tgctgatttt
    14941 tcaaaaaaat ctcaagaaga tagaacagct ttattatcag atttgcaaga agagtttgga
    15001 agacgcttta tcattttccc taatcctatg tatggttcat gggaaggtgc catttataaa
    15061 ggtgaaaagc tggatgtgct taagcaacta gaggaacgcc gtaaaagttt aaaaagcttt
    15121 aaataaagta attgaaaaag aggtgcaaaa gaaaatgtaa cctcttttta agtactatta
    15181 ttttctgaaa atagggttaa tgactgaaaa aaatgttgat tttgtgaaat gattatttta
    15241 ttattttcat attttatctt tatgctagga attgtggtat aatggaggag tataaaaaat
    15301 attaaggagt ctattcacat ggctaaattg actgttaaag acgttgattt gaaaggtaaa
    15361 aaagtcctcg ttcgtgttga ctttaatgtg cctttgaaag acggcgttat cactaacgac
    15421 aaccgtatca ctgcggctct tccaacaatc aagtatatca tcgaacaagg tggtcgtgct
    15481 atcctcttct ctcaccttgg acgtgttaaa gaagaagctg acaaagaagg aaaatcactt
    15541 gcaccggtag ctgctgattt agctgctaaa cttggtcaag atgttgtatt cccaggtgtt
    15601 actcgtggtt caaaattaga agaagcaatc aatgctttgg aagatggaca agttcttttg
    15661 gttgaaaaca ctcgttttga agatgttgac ggtaagaaag aatctaagaa tgacgaagaa
    15721 cttggtaaat actgggcttc acttggagat ggaatcttcg ttaacgatgc atttggtaca
    15781 gcacaccgtg ctcacgcatc aaacgtaggt atttcagcaa acgttgaaaa agctgtagct
    15841 ggtttccttc ttgaaaacga aattgcttac atccaagaag cagttgaaac tccagaacgt
    15901 ccattcgtag ctattcttgg tggctcaaaa gtttctgata agattggtgt tatcgaaaat
    15961 cttcttgaaa aagctgataa agttcttatc ggtggtggta tgacttacac attctacaaa
    16021 gctcaaggta tcgaaatcgg taactcactt gtagaagaag acaaattgga tgttgctaaa
    16081 gacctccttg aaaaatcaaa tggtaaattg atcttgccag ttgactcgaa agaagcaaac
    16141 gcatttgctg gttacactga agttcgcgac actgaaggtg aagcagtttc agaaggcttc
    16201 cttggtcttg acatcggtcc taaatcaatc gctaaatttg atgaagcact tgctggtgct
    16261 aaaacagttg tttggaatgg tccaatgggt gtctttgaaa accctgactt ccaagctggt
    16321 acaatcggtg tcatggacgc tatcgttaaa caaccaggag ttaaatcaat catcggtggt
    16381 ggtgattcag cagctgctgc tatcaacctt ggccgtgctg acaaattctc atggatcagt
    16441 accggcggag gcgctagcat ggaacttctc gagggtaaag tattaccagg tttggcagca
    16501 ttgactgaaa aataatagtc attataatat atagtaaagg gcatgctgat gcatgtcttt
    16561 ttttagttga tcatgtagag ttattgaact ttccctaact tcacttatca tttactagtt
    16621 tttagatata gtggtatttg gctgacactt caattttcaa aagtttatga cgatttcata
    16681 tcaattggtt tataaaagat atcctaatca taaataaaac gtttaccaaa agggttttat
    16741 ggattgatat gttaaaatag atagtgaagg gataggtatg aaaacgttaa gaaaattatt
    16801 atctaattac aagtttgata ttaaaaagtt taaattggga atgagaacat tgaaaacagg
    16861 cttatcggtc tttttagttt tattggtttt tcatttattt ggttggaaag ggctccaaat
    16921 tggtgctcta actgctgtat ttagtttaag agaagacttt gataaaagcg tgcactttgg
    16981 cttttctaga attattggta atagtatagg tggcctactc tccttagtat tttttgcatt
    17041 caatgagata tttcatcaag ctttttgggt tactttatta attgttccta tttgtaccat
    17101 gttatgtatc atggttaatg ttgcatgtaa taataaaaca ggtattattg gggcagtggc
    17161 tgctcttttg attattacac tgtctattcc aacaggacaa acgtttattt atgtgacttc
    17221 gcgggtattt gaaacatttt gtggcgtctt tgtagctatt ttagttaata ctgatgttga
    17281 acttattaaa aataaatggc ttaacaaaaa acacagtgat gttagataat ataacatata
    17341 tacttgacag gttatttttt tcagcctata ataaggaaga tgaaaggagt ttgtcatgaa
    17401 agaaaaagaa cttagacgct caatggctgt ttttcctatt ggaacggtga tgacattaac
    17461 agatctctct gcgagacaaa ttcgctatta tgaagatcaa ggtttaatta agcctgaacg
    17521 aacgcaaggg aatcggcgta tgttttcctt aaatgacatg gaccgacttt tggaaattaa
    17581 agattttttg tctgagggat taaatattgc agctattaaa cgcgaatatg tcgagcgtca
    17641 aggtaagtta atgcaaaaac aaaaagctct aacagatgca gatgtcagac ggatcttaca
    17701 tgatgaaatg ctgacacaaa gcggtttttc tactccctca cagcatattg gtaattttcg
    17761 tatttaagat atgaattgta taaaatttaa aggagaccta tttaatggca atcacagtag
    17821 ctgacattcg tcatgaagtc aaagaaaaaa atgtaacgtt tcttcgcttg atgttcactg
    17881 atatcatggg cgttatgaaa aatgtggaga ttcctgcaac gaaagaacag ttagacaaag
    17941 tattgtctaa taaggttatg tttgatggtt catctatcga aggttttgta cggatcaatg
    18001 agtcagatat gtacctttac cccgatttag acacttggat tgtttttccc tggggagatg
    18061 aaaatggagc agttgcaggt ttaatttgtg atatttatac agcagaagga aagccttttg
    18121 caggagatcc tagaggaaat ttaaaaagag ccctgaaaca catgaacgag atcggctaca
    18181 aatcatttaa tcttggacca gaaccagaat ttttcctttt taagatggat gataaaggta
    18241 atccgacact tgaagttaac gataatggtg gttattttga tttagcgcca attgacttag
    18301 cagacaacac gcgccgtgaa attgtgaata ttttaacgaa gatgggtttt gaagtggaag
    18361 ctagtcatca tgaagtggct gttggtcaac atgagattga ttttaaatat gcagatgttt
    18421 tgaaagcttg tgataatatt caaattttta agctagttgt aaaaacgatt gcccgtgaac
    18481 atggacttta tgctactttc atggctaaac caaaatttgg aatagctgga tcagggatgc
    18541 actgtaacat gtctttgttt gataaccaag gtaataatgc tttttatgat gaagctgata
    18601 agcgaggaat gcagttatca gaagatgctt attatttctt gggaggacta atgaagcatg
    18661 cttataacta cactgctatc actaacccta cagtgaattc ttataaacga ttagttccag
    18721 gttatgaggc acctgtttat gtcgcttggg ctggaagtaa tcgttcaccg cttatccgtg
    18781 ttccagcatc acgtggtatg ggaacgcgtt tggagttacg ttcggttgat ccgacagcta
    18841 atccttattt agccttggct gttctcttgg aagctggatt agatggtatc attaacaaaa
    18901 ttgaagctcc agaacccgtt gaagctaaca tttataccat gacaatggaa gaacgaaatg
    18961 aagcaggcat tattgatttg ccatcaacgc ttcataatgc cttaaaagct cttcaaaaag
    19021 atgatgtggt acaaaaggca ctaggttacc atatctacac taatttctta gaagcaaaac
    19081 gaattgaatg gtcttcctat gcaacttttg tttctcaatg ggaaattgac cattatattc
    19141 ataactatta gacttatcag gacctgctca agttgagtgg gtcttttgga tgagctattg
    19201 tatttgagct agcaagctat tattagctga tcggacacca attgattttc cattatagaa
    19261 aactaaaaac cagtccatgt tctatgagga ctggttttta gtttgataat tatctgaact
    19321 agaactgata tattaaagat atagacggat agtattaaga aatgagtctt atttgatttt
    19381 agtgcttatc aggtgttaag accatcggaa ttataattgg ttcacgctca gtcttttcat
    19441 acaagaatgg acgaagagca ttgacgatag caccgttaac tgattggatg ctagcatcct
    19501 tattttttaa agcaatacgg atagcgttaa agagcacacg ttggctttcg cggatcaagt
    19561 cacctgactc acgcatataa atgaaaccac ggctcagaat atctggtcca gctaaaatca
    19621 tttgtgtgtt aaaatcaaca gttgcaacag ctaagacaac accatcttct gacaaatcac
    19681 ggcggtcccg taaaacggct gctccaatat ccccgatgcc attaccatcc acgtagatgt
    19741 cttgggcatt aaagtgacca gcaatacgcg cactgtcact ggttaaagct agaatgtctc
    19801 cattttccat aataaagata ttttcttttg gaataccaat atccatcgcc agtcctgcat
    19861 gaactttttg catgcgatac tcaccatgta ctggcatgaa atattttggt ttaatcaaac
    19921 taagcatgag cttttgttct tgctgtcctc catgaccaga agtatgaata ttattgactt
    19981 ttccatgaat aacatcaaca ccagcttctt ggatggtatt gattaattta ttgacacttg
    20041 tggtattacc agggattgga cttgatgaga aaatcacagt atcaccaggc tgtaaagtta
    20101 cttgacgatg agtgccatta gcgatacgag caagtgctgc catagactca ccttgactac
    20161 ctgtacacat aattaaaact tcactagcat gaagattttt aagttcgctt ggctcaatga
    20221 aggtaccttt tggaacttta atatagccaa gttcaatacc gttaacaatt gccttttcca
    20281 tagaacgacc aaaaacggca attttacgtc ccgttttaac agcagcttct gcagcctgtt
    20341 gcaaacggta gatgtttgaa gcgaaggaag caaaaataat acgcccatga ataccttcga
    20401 taatttttaa aatggattgg ccaacaactt tttcagagtt agtaaaggtt gggatttcag
    20461 cattagttga gtctgataat aagcaaagga caccttcttc accaagcgct gccatacgtt
    20521 gtaaatctgc aggatctcct actggcgtaa agtcaaattt aaaatcacca gtacagataa
    20581 tcttaccttg aggggtgtgg ataacaatac cgaccggttc tggaatggaa tgagtagtct
    20641 tgaagaaagt gacgctcatg tttttaaaag ttaactcggt gttgtgattg atttcgtaaa
    20701 cagttgcttc acgccaaaga ccatgttctt ccaatttacc acggataaga gcaagtgcta
    20761 aaggtcctgc gtagataggg atattagcct gtttgagaag aaatggaata ccaccaatgt
    20821 ggtcttcgtg accatgtgta ataacaagag ctttgacccg gtctaagtta tcgacaatgt
    20881 aagaatagtc tggaataacg taatcaattc ccaaaagatc atcttctggg aatttgatac
    20941 cggcatcaac gataatgatc tcgtcttgat actcaatacc ataagtattc ttcccaatct
    21001 ctcccagacc accaatggca aacaccccaa cttcattagg ttttaaactg atatttgtca
    21061 tcttagaact ccgttaattc aaaaacaccg gtctcttttt cgtaatcgag gtgtttgtca
    21121 gaaagtagct cgataaattc cacgttataa taggtgttat cttcaacgag acgacgagcc
    21181 ttgatacggc catcgagttc atcagtagca tcaatattaa gatagagtgc tttagtactt
    21241 tcacgacgtg ggctttgatc ctttgtttct tgatagaaaa ctttataaat catttgtttt
    21301 ttattccttt cgttatgaat caattatcca aacaataata aataggtcaa aaggtaggtg
    21361 ttccattttg acggttttat atgttgtcat tattgtgtag tagattaatt cctgattctg
    21421 tgcaattatt ataattatac cataaactta gcttttagta aaaaatttag gtacatttat
    21481 ggtatagtct aaatcaaatt aagagtcagc ctttttgttt atgattctta atttgtgtta
    21541 taattgatac tagaacttgc agtagaaagg cataaaatga agacacttgc atttgatacc
    21601 tcaaataaaa ccttgtccct tgctatactt gatgatgaga cacttctagc agatatgacc
    21661 cttaacattc agaaaaatca tagcgttagc cttatgcctg ctattgattt tttgatgact
    21721 tgtactgatc ttaaacctca agatttagaa agaatagtgg ttgcaaaagg ccctggatct
    21781 tacacaggtt tacgagtggc agttgctact gcaaaaacgt tagcgtacag tttaaatatt
    21841 gcattggtcg ggatttcgag tctatatgct ttggctgcgt ctacttgtaa acagtatcca
    21901 aatactttgg tggtgccatt gattgatgct agaaggcaaa atgcgtatgt aggttattat
    21961 cggcaaggaa aatcagtgat gccacaagcc catgcttcac tagaagttat tatagaacaa
    22021 ttagtagaag aaggacagct gatttttgtt ggggagactg ctccttttgc tgagcaaatt
    22081 caaaagaaac tacctcaggc gatactactt ccaacccttc cttctgctta cgaatgtggt
    22141 cttttggggc aaagtttggc accagaaaat gtagacgcct ttgtccctca atatctcaag
    22201 agagtggaag ctgaagaaaa ctggctcaaa gataatgaga taaaagatga tagtcactac
    22261 gttaagcgaa tctaatatga aaacagttga agaacaagca aaaaatattt atcagctttt
    22321 ggaaatggtt tatggcacgt ctccttggac tttagaacaa gtattaatcg atatacggcg
    22381 ggatcaaaca gattatttcc tactatatga tcatgataaa ttgctaggtt ttttagctat
    22441 tcaagattta gctggtgaag ttgaaatgac tcaaattgct atactaccta gtcatcaagg
    22501 acttgggcta gcgtctcaat tgatgacgca tttagatagc attgaaagtg acatcttttt
    22561 agaggttaga gaatctaatc atagagcaca gggtttatat cagaagtttg gttttaaact
    22621 tattggcaaa agaccagact actatagaaa tcctatagaa acagctttat tgatgaaacg
    22681 ggaaggaaaa catgacagat aggtatattt tagcagttga gagttcttgt gatgagacca
    22741 gtgtagcgat tttaaaaaac gaaagtaccc tcttaagtaa tgtcattgca agtcaggttg
    22801 agagccacaa acgctttggt ggtgtggtgc cagaagtagc tagtagacac catgtcgaag
    22861 tgattacgac ttgctttgag gatgctttgc aagaagctgg catcagtgct tcagatttat
    22921 cagcagttgc ggtaacttat ggccctggac ttgttggagc cttgttagtt ggattagcag
    22981 ctgcgaaggc ttttgcttgg gcaaatcatt tacctttgat tccggttaat catatggcag
    23041 gccacttaat ggcagcgcgt gaacaaaagc ctttagttta tcctttaata gcactattgg
    23101 tttcaggagg acacacagaa ttggtttatg tcccagagcc aggggattat catattattg
    23161 gcgagacgcg tgacgacgct gttggtgagg cttatgataa agttgggcgt gtgatgggct
    23221 taacctatcc ggcaggacgt gaaattgacc agttggctca taaaggtcaa gatacttatc
    23281 atttccctcg tgctatgata acagaagacc acttagaatt ttccttttca ggactcaagt
    23341 ccgcttttat caatcttcat cataatgcta agcaaaaagg cgatgaactg attctagaag
    23401 atttgtgtgc atcttttcaa gcagctgttc tagatatttt attggccaaa acaaaaaaag
    23461 ctctgagcag ataccctgca aagatgcttg tagtggctgg tggtgtagcg gcaaatcaag
    23521 gattacgaga tagattagca caagaaatta cacatataga agttgtgatt cctaaactaa
    23581 gactctgtgg agataacgcg ggtatgattg ctttagcagc agctattgag tatgacaaac
    23641 agcattttgc taatatgagc ctaaacgcga agcctagttt agcttttgac cagtttccag
    23701 acagttttgt aataaactag tcacacttgc tagttagtaa gatttataat ggataagaat
    23761 ctccctttga aaaaacaacc cggtatcttg gcataccggg ttgtcatgat gataagtaat
    23821 tggcttctat ggttactaat aattcagata attacctgca aaataattgt ctgcctaagg
    23881 tgaagtaagc ttaccagcta gctttagtga cgccaggaag ctgtcctttg tgagctaagt
    23941 cacggaaatt gatacggcta acaccaaact tacgcatgta agcatgagga cggccgtcaa
    24001 ttttgtcgcg gttcttcaag cggtttggat ttgaatcacg aggcaatttg cgcagtgatt
    24061 cataatcgcc cttcgctttt aagtcacgac gaaggtcagc gtactgttca atcagttgga
    24121 gctgcttttg gtatttagca atttttgact tttttgccat tgctttctcc ttcttaatat
    24181 ttaataacct cagagtcgtt atcagcgctc tgaatccact taactggtat ttaactggtt
    24241 aatcggatat ataatcatta tacaataaat aaaagcaaaa gcaactaaaa aaaattaact
    24301 agttattttt ttagccataa tatcaaaaaa ctccacatct aagtgtggga gtcaggtatt
    24361 aataactgtt taaaggtgtt taatgagtgt cgctttagca actttgagat tgaactttta
    24421 agtcaaataa atcagcagga acgtagtgtt tggttgagac caagatgtct ttaccattgt
    24481 agagagtttc ttggattaaa ataaaagcac tattttctga tagagtatac ttagttgctg
    24541 tgtaattgcc agtcttggta tagccgattt ggttgcagga atgagaagct ttaccagatt
    24601 catagatttt ctcttctaag aaagtaagaa ggggctcttc ttgattgaga tttatagcat
    24661 atttagaaat aatctcaata gggatacaag ataaactata agcaatagct ttattttggc
    24721 tatgatacca gcgatcaaca ataatcacaa caggagtttc ttgttttagg gatgctgtaa
    24781 tggcaacagt gggcacttca atccgatatt ccaactcaac ctttgtgatg tcagatgata
    24841 ggcttgcata tatggggtgt tgaagatatt cgaaaccttg gtgatattta gtttcaggag
    24901 tcttgagtat aaaatttccc tttcctctga tattttttat gagatgatcc tcttggagga
    24961 gggctaggga tttacgtagt gtcatccgac tgacattgag ctgtctagca agttcaggtt
    25021 ctgatggcag ttgcatgcca ggactatatg taccatcttg aataagttga aaaatagtat
    25081 tatatacttg cacatgttta agttttttga gttttttggt taagtcgtta gtagacatat
    25141 cacttacctc attaataatc tttagtggag tttaacttgt ctaaaaacta ttttagcact
    25201 aacagtattc cttgattaga attgtaccca tctatagtgt tagtatctca cactcaggta
    25261 ctttttgtca aaaaagagat gaaaaacacc ttaaaaaagc gtttacaaaa tagagtgtgt
    25321 gtgatactat acaagtgtat caaaaattag acaagtaaaa tacaggaggt ttttaacatg
    25381 caaaattatt caggtgaagt cggattgcaa tatcacctac agattcgtcc aggtgatgta
    25441 ggtcgttatg ttatcatgcc aggtgatcca aaacgttgtg caaaaattgc agaacatttt
    25501 gataatgcag ttcttgtagc agacagccgt gaatatgtta cgtatacagg tacattaaac
    25561 ggtgaaaaag ttagtgttac ttcaactggt attggtggtc catcagcgtc tattgcgatg
    25621 gaagaattga aactttgtgg cgctgatacc tttatccgtg ttggaacatg tggtggtatt
    25681 gaccttgatg ttaaaggcgg agacattgtt attgcaactg gagctatccg tatggaaggt
    25741 accagtaaag aatatgctcc gattgaattt cctgctgtag ctgacttgga agtgacaaat
    25801 gctctagtta atgcagctaa aaagcttggt tatacaagtc acgcaggagt agttcaatgt
    25861 aaagatgcat tttacgggca acatgagcca gaacgtatgc cagttagtta tgaattatta
    25921 aataaatggg aagcatggaa acgtctggga acaaaagcgt ctgaaatgga atctgcagct
    25981 ctttttgtag cagcaagtca tctaggagtt cgttgtggat cagatttcct tgttgttggt
    26041 aatcaagaac gcaacgcttt gggtatggat aacccaatgg ctcatgatac agaagcagct
    26101 attcaagttg ctgttgaagc actccgcaca cttattgaaa atgataaatc acaataagcg
    26161 aggttttgga agtcatcatg caatttattt atagtattat tggtatttta ttggtattag
    26221 gaattgtgta tgcaatttct ttcaatcgta agagtgtttc tctaagttta attggaaaag
    26281 ctcttatcgt tcaattcatt attgcgctaa tcttagtacg tatcccacta ggccaacaaa
    26341 ttgttagtgt tgtttcaact ggagttacta gcgtaatcaa ctgtggtcaa gctggtttaa
    26401 attttgtgtt tgggtcatta gcagatagtg gcgcaaaaac tggttttatt ttcgctattc
    26461 aaacgcttgg taatattgtt ttcttatctg ccctagttag tctactttat tatgtaggaa
    26521 tccttggatt tgtagtaaaa tggataggta agggcgttgg taaaattatg aaatcctcag
    26581 aggttgagag ttttgttgcc gtagctaata tgtttcttgg tcaaacagac agtccaattt
    26641 tggttagcaa atacctaggt cgtatgactg atagtgagat aatggttgtg ttggtatcag
    26701 gtatgggaag tatgtcagtt tctattcttg gtggctatat tgcattaggc attccaatgg
    26761 aatatctctt gattgcttca acaatggttc ctattggcag tattctcatt gctaaaatct
    26821 tattgcctca aacagaacct gttcaaaaaa ttgatgacat taagatggat aataaaggta
    26881 ataacgccaa tgtgattgat gcaatcgctg agggtgcaag cacaggtgca caaatggctt
    26941 tctcaattgg tgctagtttg attgcctttg ttggtttagt ttctttgatt aatatgatgt
    27001 taagtggatt gggaattcgc ttagaacaaa tcttctcata tgtttttgct ccatttggtt
    27061 ttcttatggg atttgaccac aaaaacattc ttctagaagg aaaccttctt ggaagtaagt
    27121 tgattttaaa tgagtttgtt tcgttccaac aattgggtca cctaatcaaa tctttagatt
    27181 atcgtacagc attggtagca actatttcac tctgtggttt tgctaattta tcaagtttag
    27241 gtatttgtgt ttcaggtatt gctgttcttt gcccggagaa acgtggcacc ctagctcgac
    27301 ttgttttccg tgcaatgatt ggcggtattg ctgtaagtat gcttagcgcc tttatcgtcg
    27361 gtattgtaac tctattctaa acgttgacaa aagaaagaag gatagtaacg tggaagtaaa
    27421 agatatttta aaaacggtag accatacttt gctagcaaca acagcaacgt ggccagaaat
    27481 ccaaacaatt ttagatgatg ccatggctta tgaaacagct tcagcatgta ttccagcctc
    27541 ttacgtcaaa aaagcagcag aatacgtttc aggtaaatta gctatttgta ctgttattgg
    27601 gttcccaaat ggctatagta caactgcagc gaaggttttt gaatgtcaag atgctattaa
    27661 aaatggtgct gatgaaattg acatggtcat taatttgaca gacgttaaaa atggggattt
    27721 tgatactgtt gaagaagaaa ttcgtcaaat caaagccgca tgtcaagacc atatcttaaa
    27781 agttatcgtt gagacatgtc aattaactaa agaagaactt atcgaacttt gtggagttgt
    27841 cacacgttca ggtgcagact ttattaaaac ctctactggt ttttcgacag caggtgctac
    27901 atttgaagat gttgaagtga tggcaaaata tgtcggcgaa ggtgttaaaa ttaaggcagc
    27961 aggtggaatc tcatcattgg aagatgctaa aacatttatt gctttaggag cttcacgctt
    28021 gggtactagc cgtatcatca agattgttaa gaacgaagct acaaaacccg atagctatta
    28081 acttaatcat accgatttag cttgagacta aaacaaaaaa ctcatgtaat aggagttaaa
    28141 aagcctacta tcttagtgat aataggcttt tttagctatg aagacttccc tgcgatccat
    28201 cctgaacaaa gagctgaggt aatattaaac cctccggtgt gagcattaat atctaagacc
    28261 tctccagcga aatacaggcc aggaactttc ttgctttcca aggttttagg gttgatttct
    28321 tttaaatcta cgcctccttt ggtcacaaac gatttagcca aagacatttt tcctgtaatg
    28381 ggtatctgga ggtgtttgag tttatcaagc aattcctttt cttgttttgg agagagttgt
    28441 ttgacctttt cggggtaatc ttctgataaa aaatcagtta ctcgttcagg cagtaggcct
    28501 ttaagagcat ttttgatgtt tttatcacgt tgatcactca gataagcggt taagtcatct
    28561 gttgataagt gtggcaaaaa gtccagctct gcaatttctc cacctttcac aaaagaggac
    28621 agacgaagag ctgcaggtcc tgaaaggcca aagtgggtga agagtaagtc gtgtgtgatg
    28681 acgtgtttgt cataactaag agtaacgtcg tctagggaaa taccttgcaa gactttgtga
    28741 ggaaaatcag ttaacagagg gctctcagca gcttctaaat cagtcacggt cagtttaaaa
    28801 tgccgtgcaa tatcatgtcc aaaacctgtt gaacccgttg aagggtagga cttgcctcct
    28861 gtggtcacaa tcaatttttg gcaggtgaaa gtctggtctg cagatttgag gtagaagagg
    28921 tcatcttgtt ttttaacgga aaccacttca gtgctggtta gcacctgacc cccaagagcc
    28981 ttgatttttt tctccagagc atcaatgatt gtccttgatt tatctgttgt ggggaacatc
    29041 cgtccatggt cttcttcttt gagtttgacg ccgttttcct cgaaaaaggc aatgatgtca
    29101 tggttgtcaa actgggaaaa aacactatat aaaaagcggc catttccagg aatgccagcc
    29161 ataaggacat caaggttacc actattagtg acattacagc gtccaccacc ggttcccgct
    29221 aattttttac ctaagcggcg atttttttca attaagagag ttttatagct atagtagctg
    29281 ctggagatgg cggccatcat accagcaggg ccacctccaa taataatcgt atcgtattga
    29341 gtcatagcct tattttagca taaaagcggg ggatttaata gtcaggtaaa tgaactaaca
    29401 tggcataccg ttttttacgc atttaaacaa tgcctccatg acatcaaaaa gctcgaaaaa
    29461 tagtccagat gattaaaatt atctttgtat gaaaacggtt gcttttttat ttaattcatg
    29521 ctattatatt actaaagaat gaagtaatca ttaagttctt ttgtaatttt ttaaaaatta
    29581 aataatttta ttaaaggaga atagtatgaa aaaaagattt aatagcaaag cgataacttt
    29641 gcttgctgta agctctttaa gtttgggatt tacggccgta actagtgttg tcagtgcggc
    29701 aacagcacat ccagctccat ctccaattat tgatgaatgg aattatggag taagtgacaa
    29761 ctatggttat tctaattatt atgttaaatc tccaaacaat attggttcaa aatcttcgat
    29821 aactaattat tggggatcca ttaaggcatc tgattctcaa aaatacgggt gggcactgtc
    29881 aagttcaaaa aaagcttgga atgatattcg gttgaatgca cattggaatt actttaattt
    29941 ttagaaatgg tatatgcaag ctaaaggccc aataattatt atagggtctt ttataatata
    30001 ggaagttgtt tatgaaatta aaattatctg tcattggatt attcttttgc ttaattgctg
    30061 cgataggtgt tatcagtatt agtgatactc aaaaaccggt acctttacca attgatggtg
    30121 ccttttcaat ccaaggaaaa agtagccttt ctagtgacga agtttacaaa aagattagta
    30181 atctatcaaa aacgaaaaac gttaatattt acaagcctat agttcgtaac tcaggtcagt
    30241 tgacttatat caattttaaa aatacaaatt atgaacaatt aaaatctgca ccgataacag
    30301 gcatgtatta taccttaggt aaaatagatt caagtagctt aaaggtactt acagcttcag
    30361 gtttgaaact tgtttatgca gcgtatcctt ggtatatagg ggggatttta caatttgatg
    30421 ggactttaag gttgttattg atgctatcaa tttacttaac attattagtt gttttatttg
    30481 ttgttagaac gaggcagatt aaagaaggag tgataagaca ttctttagga ttgcctatat
    30541 ataacttaag aagagattat gtcatttctt ttacttttga attggttatg gctggtttat
    30601 taatgattgc ctacagctat ttttggggaa gtggcctctt tacttacagt tctaagttat
    30661 ttttctctct aattttaact aatcttataa tattccagat tgttgacatt atcactttta
    30721 ttttattttg gctaactatt agggttgaga aacctattga aattataaaa aataaagcaa
    30781 aaaatagttt tctttttatt gtatggcttg taattatttc tattataata atcgtctcag
    30841 gaatcttttt acaagaaaca aaggctagtc aatcaaggat aaatagacag ataagtcaac
    30901 tatccccatg gaacaaagta agaaactgga aaagactaga gttgttgggg attgaaaatg
    30961 gctctatcaa taatggggaa ataaatgagc cagaatcaca atatatacag atagcgacag
    31021 ccttaaaaaa attagatttt atatatatca aaccctcttc agtatatatt ccagattaca
    31081 tgaaaaatac taactttgca gaagattttt tcaaaaaatt aaataatgat ggtataacgg
    31141 atccagaagt aaataaagag atgatttata tcaataaatc gggcgctaat attcaaaata
    31201 aagtcaatgg aacagactat cagctgttag ataataaggt agcaacaatc tatattcctg
    31261 ataaatttaa agagaaaaag aaatcaattg aaaataccgt tgtggcagag caatttactg
    31321 gaactaagta tacaaaagaa aaccttgcag tgcagataat tcctaatgag aaaaaaattt
    31381 tttattttaa tgagaatgga aacgagcatg atgaaaataa agaagcctca cctatggcaa
    31441 atgtaacaga tagtaaaggt aatatagtcg ttgtgttaga tactgataaa atggtagcaa
    31501 gcaaagattt ttctttggct agcaatatag taaatagctc tctttttagt cctgaagcaa
    31561 taaaaaaaat taatgacctg agtctacatt taaatttttc aattaatcca gtagatgttt
    31621 accaacttgt aaaacttaat attcaatcct taaagcacca aattttcctt tcgaaagttt
    31681 tgcagaaaat tatttacggc attgtatttc tactaattta tcaatatgct caattattta
    31741 tttcttcaaa acagaatgac tatgtgaaaa aaataatttt aggcctctca aaaacacgca
    31801 tagcaatttc gagtctcaaa tattttatta tgactattgc aatagttata atatgtacat
    31861 ttatggtgac agagcagatt gagttattat atattggtat tgcttcatta gtggttttaa
    31921 ttctttcgac agtaatgagt tttagaaaat tatcaaaaaa atacactcaa attttaaaag
    31981 gagatgaaca atgacaattg aaattttcaa cgttacaaaa aaattcagct caaaaaaaat
    32041 atttacagat ttaaatttaa cgtttgaagc tggaaaaagc tatgccttga ttggaggttc
    32101 tggctctggt aaaagtactc tgcttaacat tataggaaga ctagaaaaaa ttgataacgg
    32161 gaaagtttta gttgatgaac aagatatttg gaaaataaaa gaaagaactt tctttaaaaa
    32221 tattatcggg tatgtattcc agaattattc tttaatagaa aacaaaactg tatatgataa
    32281 tttgaaactc atcaataaag acaaaaaaaa aatatctgct gtacttgaaa aagtagggct
    32341 ctctagtgac tatttaaatc aaaaaatata tgaattgtct ggagggcagg ctcagcgtgt
    32401 agctattgca agaatgttaa tgaagccacg taaaataatt ttagcagatg agccaacagg
    32461 agctttggat ggtgaaattg gaaaagaaat tattaatttg ttgttgagcg aaacagccga
    32521 aaataaatac gttattatag caactcatga tccagctgta tataataagg ttgattttat
    32581 aattgatatg aaagatatag ggagtaagaa atgaaaaaga aaatttatat tggcttgatt
    32641 gtcattgttg gagtttttgt agtgttcttg tctggaaaac aaatcacaca aaaagaaact
    32701 tcaaataagc ctgaggtgac cctaacagtt tataccatct cctctagtga tacccaaaaa
    32761 tggagtaaag taaagcagtt tgaaacgaaa gaagcaatat acctagtaaa ggtaaaagaa
    32821 actacctctt cagaagaagt cttttcaaac atcattgcag atggaatggc agttggtttt
    32881 ggagttagtg aagaagacgt taaacagttt aacgataatt tatgtagccc ggtagaagat
    32941 gctaagaata ataaattaat tggaatagaa ttcatcaatt tttctagtgt tgataagggg
    33001 tttgtggttg caaattttga ctacggcaaa aaagcgttta attctcagaa aacagaaaat
    33061 aaaaaattat ataaaaagct ctatgaagcg tataaaggat agctatttat agtttcttga
    33121 gagtgatacc acttctgaat aataaatgaa ctaacttggc ataccgtttt ctattcatct
    33181 aacaatgttt tcaagaaata attgatgcct ttagcacggc tgaaatgagg tcaaattgac
    33241 agaatactcc tattatggta ctatttttaa taagaatatt cagtgatagt gataaagagg
    33301 aaaatatggg aattgaaaag acagttagtg aactagctga tattttggga gtgagtcgtc
    33361 aggccgtcaa taatcgtgtt aagtctttac ccgaagaaga tcttgacaaa aatgaaaaag
    33421 gcgttactgt ggttaagcgc agtggtcttg ttaagctgga agagatctat aaaaaaacaa
    33481 tttttgacga tgagcctatc agtgaagaaa cgaaacaacg tgagctttta gagattcttg
    33541 tggatgagaa gaacactgaa atcacgcgcc tttatgagca actcaaagcc aaagatgctc
    33601 aacttgcctc aaaagatgag caaatgcgtg ttaaagacgt gcagatcgct gaaaaagata
    33661 aacaattgga tcagcagcaa caattaactg ctaaagccat ggctgataaa gaaaccctaa
    33721 aactagaatt ggaagaagct aaagcagaag ccaaccaagc tcgcttacaa gtcgaagaag
    33781 ttcaggctga agttggccct aaaaaaggct ttttaacccg cttgtttgcg aaataatcac
    33841 aaaaaatgac gaaggcccta tcagggtctt ttttggagga aaacatgaaa cacttagata
    33901 cttatattgc ctttgattta gaatttaaca ctgtcaatga cgttagtcat gtcattcaag
    33961 tatcagcagt caaatatgac caccataaag aagttgacag ctttgacacc tatgtttaca
    34021 cagatgttcc cttacagagc tttatcaatg gcctaacagg aatcacatct gacaagattg
    34081 ctgcggcacc aaaagtagag gaggttatgg ctgcgtttaa aaattttgtg ggagagttac
    34141 cactgattgg ttataatgct caaaaatctg acctgcctat tctagctgaa aatggcttag
    34201 atttaaggga ccaataccag attgacctct ttgatgaagc ttacgataga cgtagcgcag
    34261 acttaaatgg gattgctaac cttcgtctgc aaacagtggc aaccttttta ggcattaagg
    34321 gtcgaggtca taatagctta gaagatgcca gaatgacagc agcaatctat aagtcttttt
    34381 tagaaacaga tacaaacaaa gcatatctca gccagcaaga agaggtaacc acagataacc
    34441 ctttcgcagc tttgggagac ttttttgact aagggctctg ctatttctaa aaaataacaa
    34501 aaattcctag ggttagctct tgcttgtaac gctaggtcat gtgataagat aggggcatat
    34561 caagagggag tcgagtgcac acgaatcgtt tttgatcact aaatgataga aagaaggtgt
    34621 ggaaatgtct caattctatt cgactggaga attggccaac ttagctggcg tttctattcg
    34681 aactgtccaa tactatgatc aacgtgggat tttgattccg acagctttga cagaaggtgg
    34741 acggcgactc tatacggatt ctgatttaga acaattacgc atgatttgct ttctgcgcga
    34801 tttgggattt tccattgagc agattcgcaa agtcttagca gaggaaaatg cagcccaggt
    34861 gctggaattg cttctagttg atcatattgc gactgccaag gaagacttgg ccgcaaaaga
    34921 gcaacaagtt gacatagctg tcaagatact tgacagactt cgaaaacaag acccccagtc
    34981 actagatttt ctgatggaca tttcgcttag catgaaaaat caaaaagctt ggaaaaaatt
    35041 acaatggaag atgggtatca gtatattttt agccattgtt tcatatgtag gacttatttt
    35101 actatctagt tatctgcaaa aattttggtt agtttatgtt gctatgggac tatttctacc
    35161 aggtttcagt tggttggtaa tttattttag gaagcaattt atttatctct gtccccattg
    35221 tcatcagaaa tttgaaccta gctttaatga atttgccctt gcaggccata caccacgaac
    35281 cagaaagtta acctgctccc attgccatca aaaatcgtat tgcttggaac tggcaaaaga
    35341 ggacactgtc tcaagaagat aagcactaac tcataaaaaa actagcctga gatccttctc
    35401 aagctagttt ttgttttttt agaatggtaa tttaccagca aaagcgccta atgttttctt
    35461 agttgtttca tcaatttgcg taagggcatc attaatggct tgagtggtca tgtcttgaag
    35521 cgtttctaca tcttcaggat ctacaacagc ttctttgaag tcaatcccaa ccaatttttt
    35581 atcacccgtg aaagtggcag tgacaaggtc ttgagctgat tttccagtaa attgcatagc
    35641 agccaaatct gcttgttttt gttccatttg tttctgaagc ttttgtgcct gcttcatcat
    35701 attctgcata ttcatcataa gactatactc tccttaattt aatactaact attataacat
    35761 ataaaaagag gcaataaaaa caatgtcagt tagtagacta gcctgtatca gtaagaaagt
    35821 cgctatggat aagatggatt agcagcaagg gtctggctaa tgacagaata agtctagaaa
    35881 tgaactattt ttgtcataga ctagtccgac ttagtggtta tctaattcac gatcttcttt
    35941 agcttgttgt tctaaaagaa tttgtgcgaa gtcaacccac ttagtgtagt tattttcgga
    36001 taattgttct gaaatcatcg ctaactctcg ttgtggcaac tgataagaga ctcccttatt
    36061 ttctaaggtt aaatcttctt ccattagggc ggttaaacta acatcaaaga tagcagcaat
    36121 atcacttaat agaccagact ttggagtata ttttcctttt tcccactcac tgattgaaga
    36181 agagcttttt cgtcctagga gattagcaag ttctaattgt tccatgttct tttttaatct
    36241 aaggtacttt aaattacttg caaaaaaagt tttcttggcg gacatatttt tctccttttc
    36301 tttatataaa atatgatacc ataaaagagg ttaaagcaaa aggtatcgta aaagatggtc
    36361 taaaaactct ctaaaaactt gacttcataa aaaccgaagt ttataataaa cttaacatga
    36421 ttaactaatc tcattttagt gtggagggaa aattgtgacc aaatcaactt taaaagagtt
    36481 aagaacaaca aagaaaatga cacagcagga attgtctcac ttaacaggta tttctgtcag
    36541 aacgattgct aggtatgaaa aagatactaa aaagctaagg cgagcaaaat atgagaagct
    36601 aaaaggtatt gcacaagcat tagcggtttc cgttgatgat atttttttag gaatagattc
    36661 ggaatttatg aactaagacg atcgttttct gtttgctatt agtaagacca attaacaaaa
    36721 aagtgcttag ttatattgct aagcactttt ctttgttaat ttgatgctat tggcacaatc
    36781 aattgagact gactgagatc gatagttaaa gcatagttgc tgttgtcacg gacagtatgt
    36841 tcaaaatcgg ttgtgtataa gatgactcga agggtatctc ctttttctag atgatagatg
    36901 gttggttgca aaggaagtct aacagtcatc catttattat taggaatagt ttcaattgac
    36961 gacaaattat tgcgattttg taaattcatg aaacctttcg aaataacacg gtaagaattt
    37021 tctctaaatg gtagctcttt aaggggttct ctagagaaat tttgcccatt gtcaatactt
    37081 gattgggtga gagcaatagg aagatcgccc aagcgttttt tcttaccata gtcaaggatt
    37141 tgtgcagata gaagtccttt attttcactt gatttcactt ttaactgtag gacaatttca
    37201 ccgttaataa gtaagtcctc ttccagtaaa atatcaacca gggcttgatt ggccttgcct
    37261 ttaaagagag ctgctttgaa cgcacggaaa tctttaccgt aagctttaaa ttcatcttca
    37321 ccatagtgat tatcaattaa aagcagttcc tgaccgaggt caagctcttt gatggttgag
    37381 gttccaaaga ctttacgctc ctgccagttt tgtgggcagg tgttatcctg ccagatcatt
    37441 tcaggaaggc taaaatcatt agctaggcct aagagttttt gacataaaag ggcattcata
    37501 ctttcgcgaa aatcaatgga ttgccaatta tgcatgtaaa catgctcacc ttgatgtaaa
    37561 aagaggtgct tgttgattgt ggaaggaagc gcattgacaa tctcatagac ctgtcttggt
    37621 ttgacattcc agtcttgtaa gccatgagta taaacgacat cacactttat ttgatgagca
    37681 ttttttaagt aattgcggtc gtgccaaaat tgattataat caccagattg tcggtctaac
    37741 gcctgagact gttggtttag caattcttga taacgatcat tatggcgaag ataatcgcca
    37801 gccaataaat tacgcgagta ggtgagctca gttaggacat ctagatcctc tccaggatag
    37861 ccaccaggac tacaaactaa gccattttcg cggtagtagt tgtaccaaga agaaatagct
    37921 gattctgcaa taatcatggc taatccgtct acgcctgtag tggctaaacc agtagacata
    37981 gttcccaagt aggactttcc agtcgtacaa actagaccat ttgcccaatc agctctcact
    38041 tggtgggttt ttgagtggct ggtgtaggct gtggctcttc catttagcca atcaataacg
    38101 gctttaaagc tttcaatttg agcatagtct ccactcgtca taaagccagt ggaaccagca
    38161 gttcccacac ctgaaacata gatattagca aacccacgtg cgaggaaata atcgttgagg
    38221 ctataggagt taatgtagct aaagctttct agattttgac ctataggaag tttgcaaggc
    38281 tgtgtctcaa gaggaataaa atccctgtct tcgacagtga ttttattcct ttttttaacg
    38341 actaactctt tttccatacg ataaagtttt ttgtcgttgg ctacttcatt gataccttga
    38401 tggtatggac taggtgtcat caaagttggt aatggttttt gagattgagg acgaatgata
    38461 ttaaccttaa taagatcaag ttggccatct tgatcggtat caagaggtgc ttcaacgtaa
    38521 acaacttctc gaatcagctg tgacgtgtca aaggtagcta atgatttacc gttgaagaag
    38581 tggtaatcat tgtccatagt aagcattcct tggctgacca aatcatcaat cagggtcatg
    38641 ccagacttac aacgggtcgc taataagtgg tggatatgct tgataatttc tgtttcgtca
    38701 aagtggatag gaaaggccag tttatcaagg aaagcatcgg cctctgtaaa atcaactcca
    38761 ggaataaagc caaggacttg cagggccagt aaatcaaaaa cctctttgga taactcttgc
    38821 tctgattgaa aaaagctcaa tgcgtccatg tcatttttgg cgattagatg acttaaagga
    38881 taatcactgt cagggtagtg gaagaagagt ttacgtaaaa agctttctag gttagctttg
    38941 gcagtttttt gcagatccag atcaaagccc aattctttta attcttcagc cgctctttct
    39001 agactagttg gaatatagga aaattgatta tagcgcatag gagtttcctt tctagtggct
    39061 tagaaaacga tattatcttt taatttgtca agattggtaa tgataatatg ttgtttatca
    39121 atgcgaatga tgtttttttc tttcaactgt tttagaatac ggctgacgct gctagcggta
    39181 gaaatgccac agaatttacc gagttcctca ttagtaatga caaaattaat taaaatttgg
    39241 ccatttggta gttcttcgcc aaatagggtc attaagtcat atatttgagt agaaatggca
    39301 ccgatacggc cgttagttaa catacattgc atttttttca tggatttttg aaggcgatta
    39361 tggtaaaagt ccttcacata tccttgtagt tcaatatctt cgtttatatc ttttaaaaat
    39421 gctgagcggc gaaccttgta aaaactagct ttctctgact cgatgcgaac attataaggc
    39481 tctcccatat cttttgaata accagtgttg agaacagaag taatttcaag tcctgtaaca
    39541 tatctcaaat taaattccat tccatatttt gaaagaacac tctgcttcac aatgccgtct
    39601 tttaaaatat aagtgaagtc ctcttcgaga ctttcaaaag tcaagtactt gtgataactt
    39661 ttttcaatga taggaaagtt atgattatca atataccgtt gcaaaatgtg atgattcacg
    39721 cttttctcca aatattcttt taccaatgta taatttgtat cttcaagaga aattaacatt
    39781 tgctaatgcc ctttgatagt aacacttata ttataccatt gaaatatatt atattctgct
    39841 ttttttgtaa aatgtttatg aaaatagaga ggagttcatt ttcaatggaa gaaaaattgt
    39901 ttaacaaaca ttttgtggcc atcacagtaa taaactttat agtctatatg gtctactatc
    39961 tttttacggt tattattgcc tttgtggcga ctagagagct aggagctcag actagccagg
    40021 caggattggc aacagggatt tatattctag ggactttatt agcccgtttg atttttggaa
    40081 agcagttgga ggtgtttggt cgtcgtttgg ttttaagagg aggtgctatt ttttacctct
    40141 taacgacctt agcctacttt tatatgccaa cgattagcat gatgtacttg gttcgtttct
    40201 tgaacggatt tggttatggt gtagtatcaa ctgcgacaaa taccattgta acagcctata
    40261 ttccagcttg taaaagagga gaaggaatta atttttatgg cttgtcaact agtctagcag
    40321 cagctattgg cccttttgta ggaaccttta tgttggataa tcttcatatt gattttcgca
    40381 tgattattgt cttatgtagc gtcttgattg ggtgtgtggt tgttggtgct tttgcttttc
    40441 cagtcaaaaa catgtcttta aatgcggagc aattggctaa aaccaaatcg tggactgtgg
    40501 atagtttcat tgagaaaaaa gccttgttta tcactgcaat cgccttttta atggggattg
    40561 cctatgcttc agttttagga tttcaaaaat tatacacgtc tgaaattcat ttgacagcag
    40621 taggtgctta tttctttgtg gtctacgcct taatcattac catcactcgt ccagcaatgg
    40681 gacgtttaat ggatgctaaa ggcgataaat gggtcttgta cccaagttat ctttttttag
    40741 caatgggact cttcttgcta ggtagtgtat cgtctggagg aagctatttg ctttcaggag
    40801 ccttgattgg ctttggttat ggcactttta tgtcttgtgg tcaagccgcc tccatacaag
    40861 gagtggatga gcatcgcttt aatacggcta tgtctactta tatgattggt cttgatttag
    40921 gtttaggtgc tgggccttac ctcttgggct tgattaaaga tttggcactt ggtagtggtg
    40981 tggcttcctt taggcactta ttctggttag ctgctgtgat tccactgatt tgtaccttgc
    41041 tttacttgtt aaaaacaaag acaagacagg ttgtgtctta aggacaagaa aaggagttta
    41101 gtcttatact taaatacaaa cgtatcttaa cagctgtaga tagttcctat gagccgggat
    41161 tagctgtggg taaagccgtt actgtagcgc tttgaaataa tcctagtttg ctattggtac
    41221 atgtcaataa aaatgactcg caaaagccat tcgtgaaaga gaaaatgtgg ccttattttt
    41281 agtaggtgca attggattaa catcagaata taggatttgt catgttaaga ttgatctgtt
    41341 gatagtgaga gctaataaaa aatgttataa tactatgaaa taaagtgata aagataaggt
    41401 cttttatggt ccttttcttt accgctttta ttattgtgtg aaaataacac ttaatgatca
    41461 ttttatttat tattttatta cctttacatt catcaataag cctgttaaaa tggtatttgt
    41521 tatattttat tatttggagg aaaccagaaa acatggaaat gacatggact gtgaaataca
    41581 tcacagaatt tatcgcaacg gcttttttga ttattttggg gaatggagct gttgctaacg
    41641 tcgacttaaa aggaacaaaa ggacataact caggttggtt agtgattgct tttggctatg
    41701 gcttaggagt tatgatgcca gctttgatgt ttggtaatgt atctggaaac catattaatc
    41761 ctgctttcac agttggacta gcagtgtcag gcttgttccc atgggctcac gtcttacaat
    41821 acgttgtggc acaattactt ggagctatct ttggtcagtt ggtagtggta atggtttata
    41881 aaccttactt tatgaaaact gaaaatccaa atcatgtttt aggctcattc tcaacgattt
    41941 catctcttga taatggtcaa aaagacagcc ataaagtctc ttatatcaat ggtttcttaa
    42001 acgaatttgt aggttcattt gttcttttct ttggtgcttt ggctttgact aaaaactact
    42061 ttggggtaga gttagttggt aaattgattg aagcaggtta cgaccaaaca actgcagcaa
    42121 cacaaatttc accatacgtg acaggctcat tggcggttgc tcacattggt attggtttct
    42181 tagttatggt cttggttact tcattaggtg gaccaactgg gccagcacta aacccagcgc
    42241 gtgattttgg gcctcgtttg ttacaccact tcttaccaaa atcagttctt ggtcaagcca
    42301 aaggtgattc aaaatggtgg tatgcatggg ttcctgtagt tgcacctatc ttagcggcga
    42361 tagtagccgt agcagcgttt aaataccttt atatcagata aaaaaagaac taaagcttct
    42421 tttagactca agacaagctc gcttaaggaa cttgtctttt tatatgagtt aagcgaaatc
    42481 taatgcaaac ttgactgtct tgtatagcgt attttgtaaa ttttactaga cttcttgtaa
    42541 aaaagcctct cctcagataa gatgactaat gcctctaaac ggtgttacaa ataaacgagg
    42601 catccctaac ttcctttatc tggtgttgaa actattttgc acggactaaa tcactgatag
    42661 agtaaaaaac gatttttcat ttttgttgta
//
LOCUS       DAUCLR010000003        41875 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.003, whole genome shotgun sequence.
ACCESSION   DAUCLR010000003 DAUCLR010000000
VERSION     DAUCLR010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 41875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41875
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.003"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..450
                     /gene="greA"
                     /locus_tag="VGT87_000103"
     CDS             <1..450
                     /gene="greA"
                     /locus_tag="VGT87_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEQ3812995.1"
                     /translation="EKEQLEKELEELKLVRRPEIVERIKIARSYGDLSENSEYDAAKD
                     EQAFVEGQISTLETKIRYAEIIDSDAVAKDEVAIGKTVIVQEVGTTDKDTYHIVGAAG
                     ADIFSGKISNESPIAQALIGKKTGDKVRIESPAATYDVEIISVEKTN"
     gene            complement(1070..1993)
                     /gene="yidC"
                     /locus_tag="VGT87_000104"
     CDS             complement(1070..1993)
                     /gene="yidC"
                     /locus_tag="VGT87_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEQ3812996.1"
                     /translation="MKLTLNRILFSGLALSILFTLTGCVGRDAHGNPKGMIWEFLGKP
                     MSYFIDYFANNAGLGYGLAIIIVTIIVRTLILPLGLYQSWKASYQSEKMAFLKPVFEP
                     INKRIKQASSQEEKMAAQTELMAAQRAHGINPLGGIGCLPLLIQMPFFSAMYFAAQYT
                     KGVSTSTFMGIDLGSRSLVLTAIIAALYFFQSWLSMMAVSEEQREQMKTMMYTMPIMM
                     IFMSFSLPAGVGLYWLVGGFFSIIQQLITAYLLKPRLHKQIKEEYAKNPPKAYQSTSS
                     RKDVTPSQNMEQANLPKKIKSNRNAGKQRKR"
     gene            complement(2075..2353)
                     /locus_tag="VGT87_000105"
     CDS             complement(2075..2353)
                     /locus_tag="VGT87_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEQ3812997.1"
                     /translation="MQKVRLIVSGRVQGVGFRYATHTLALDIGDIYGRVWNNNDGTVE
                     ILAQSKDSDKIATFIQEVRKGPSKWAKVTYVDVTMANFEDFQDFQIGK"
     gene            2614..3351
                     /locus_tag="VGT87_000106"
     CDS             2614..3351
                     /locus_tag="VGT87_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEQ3812998.1"
                     /translation="MVITSKTNQLIKQTKKLLQKKHRKQSYLIEGWHLFEEAQKSGQV
                     FRHIFVLEEMVERLAGEQELVIVSPQVLKELTDSPSPQGIVAEVEIPKLAFPSDYKGK
                     YLVLEDVQDPGNLGTIIRTADAARFDGVFLSEKSADIYNQKTLRSMQGSHFHLPIWRT
                     DVYQLCRELQEYKTPILATTLSKKSVDYKSLTHHERLALVLGNEGQGISAEMAALADQ
                     LVHITMPGQAESLNVAVAAGILIFSLI"
     gene            3390..3890
                     /locus_tag="VGT87_000107"
     CDS             3390..3890
                     /locus_tag="VGT87_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEQ3812999.1"
                     /translation="MHYTEDKEYMEHVGHLIAHPRFQKLSHIVQHQHSTRLEHSINVS
                     YSSYKLAKRFGWDAKSTARGGLLHDFFYYDWRVTKFNKGHAWVHPRIAVRNAKKLTEL
                     NKKEEDIILKHMWGATIAFPRYKESYIVTMVDKYWAVKEAVTPLRQKWSNRRFLRRKT
                     LQSHNR"
     gene            3905..4594
                     /locus_tag="VGT87_000108"
     CDS             3905..4594
                     /locus_tag="VGT87_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEQ3813000.1"
                     /translation="MNDHVIYTQSDVGLNQFFAKIYSLVGMGVGLSAFVSYLMLYPFR
                     ENLISILVNQPMIYYGAAIIELILVFVASGAARKNTPAALPIFLIYFALNGFTLSFII
                     VAYAQTTVFQAFLSSAAVFFAMSIIGVKTKRDMSGLRKAMFAALIGVVVASLINLFIG
                     SGMMSYVISVISVLIFSGLIASDNQMIKRVYQATNGQVGDGWAVAMALSLYLDFINLF
                     ISLLRIFGRND"
     gene            4772..5014
                     /locus_tag="VGT87_000109"
     CDS             4772..5014
                     /locus_tag="VGT87_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEQ3813001.1"
                     /translation="MSTAIWILLLIVALGVGVFGGIFIARKQIEKEIGEHPRLTPEAI
                     REMMSQMGQKPSEAKIQQTYRNIIKQSKAAVSKGKK"
     gene            5192..5986
                     /gene="racE"
                     /locus_tag="VGT87_000110"
     CDS             5192..5986
                     /gene="racE"
                     /locus_tag="VGT87_000110"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEQ3813002.1"
                     /translation="MDTRPIGFLDSGVGGLTVVCELIRQLPHEKIVYIGDSARAPYGP
                     RPKKQIKEYTWELVNFLLTQNVKMIVFACNTATAVAWEEVKAALDIPVLGVVLPGASA
                     AIKSTTKGQVGVIGTPMTVASDIYRKKIQLLAPSIQVRSLACPKFVPIVESNEMCSSI
                     AKKIVYDSLSPLVGKIDTLVLGCTHYPLLRPIIQNVMGPSVKLIDSGAECVRDISVLL
                     NYFDINGNYHQKAVEHRFFTTANPEIFQEIASIWLKQKINVEHVTL"
     gene            5983..6969
                     /locus_tag="VGT87_000111"
     CDS             5983..6969
                     /locus_tag="VGT87_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEQ3813003.1"
                     /translation="MSEKIYEYKDENNWFIGKMTGHNLISGWGVKHTTIKKIDDLLDG
                     IAATLDWENPKGYDVSVVRYQSPLSLITFIIDMINQETQRKVKVTPHAGTILLMENAK
                     LLAVYLPEGGVSTATFFATSEQRFGDTVLIATRNEGKTKEFRNLFGQLGYRVENLNDY
                     PELPEVAETGTTFEENARLKAETISRLTGKMVLADDSGLKVDALGGLPGVWSARFSGP
                     DATDAKNNTKLLHELAMVFDQKKRSAQFHTTLVVAAPNKDSLVVEAEWPGYIATQPKG
                     ENGFGYDPVFIVGETGRHAAELEADQKNQLSHRGQAVRKLMEVFPAWQAKQS"
     gene            6948..7469
                     /locus_tag="VGT87_000112"
     CDS             6948..7469
                     /locus_tag="VGT87_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEQ3813004.1"
                     /translation="MASETIIVMSDSHGDRDIVQAIKDKYLGQVDAIFHNGDSELNSS
                     DPIWAGIYVVGGNCDYDTGYPDRLVTQLGTVTIAQTHGHLYHINFTWDKLDYFAQEVA
                     ADICLYGHLHRPAAWQVGQTLFMNPGSVTQPRGEINEKLYARVELTDTQIKVDYFTRD
                     HKLYPSLSKEFKR"
     gene            7466..7927
                     /locus_tag="VGT87_000113"
     CDS             7466..7927
                     /locus_tag="VGT87_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEQ3813005.1"
                     /translation="MIAKEFETFLMSHLDNYLIPEQDLAIFIDTHNADHVMLLLVSNG
                     FSRVPVITREKKYVGTISISDIMMYQSKRQLTDWEMSQTDIGEMVNTKIETISITSSL
                     TEIMHKLIDFPFLPVVDRANRFVGIITRKSILKAVNSLLHDFTDDYTIIKK"
     gene            7924..8670
                     /gene="xerD"
                     /locus_tag="VGT87_000114"
     CDS             7924..8670
                     /gene="xerD"
                     /locus_tag="VGT87_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEQ3813006.1"
                     /translation="MKSYIEPFIASKALSQNSQKAYRYDLQQFCQLVGERVNQDKLLL
                     YQNSIANLSLSAKKRKLSTANQFLYYLYQIKYLNSYFRLTDTMKVMRTEKQQAAIINT
                     DIFYQKTPFVWGQLISLLILELGLTPSEVAGIEVANLDLNFQMLTLKTKKGVRVLPLS
                     QILIPFLEQQLVGKEVYLFEHRGIPFSRQWFFNHLKTFVRSIGYEGLTAQKLREQFIL
                     KEKLAGKSIIELSDILGLKSPVTLEKYYKS"
     gene            8670..9374
                     /locus_tag="VGT87_000115"
     CDS             8670..9374
                     /locus_tag="VGT87_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEQ3813007.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSQYKVDIYEVPIVEVIEQYLNYIET
                     LQVMKLEVAGDYMLMASQLMLIKSRRLLPKVVEHIEEEDLEQDLLEKIEEYSRFKAVS
                     QALAKQHDQRAKWYSKPKQELIFEDAILQEDKTVMDLFLAFSNIMAAKRAVLKTNHTV
                     IEPDDYKIEDMMASIKQRLEKENVISLSAIFEECQTLNEVISIFLASLELIKLHVVFV
                     EQLSNFGAIILRKEKK"
     gene            9371..9922
                     /locus_tag="VGT87_000116"
     CDS             9371..9922
                     /locus_tag="VGT87_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEQ3813008.1"
                     /translation="MTYLSQIEALLFVAGEEGLSLRHLASMLSLTPTALQQQLEKLSQ
                     KYEKDQHSSLCLIETANTYRLVTKEGFAELLRAYAKTPMNQSLSRASLEVLSIVAYKQ
                     PITRIEIDDIRGVNSSGALSKLLAFDLIREAGKKDVVGRPHLYATTDYFLDYMGINHL
                     DELIEVSAVEPADEEIALFRTQD"
     gene            10043..10765
                     /locus_tag="VGT87_000117"
     CDS             10043..10765
                     /locus_tag="VGT87_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEQ3813009.1"
                     /translation="MRINKYIAHAGIASRRKAEELIKQGLVTLNGQVITDLATTVKSG
                     DVVEIEGSPIYNEEKVYYLLNKPRGAISSVSDDKGRKTVLGLLPQVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDTMIHPRNEIDKVYLARVKGIATKENLRPLTRGIVIDGKKTKP
                     ARYNIVRVEADKNRSIVELTIHEGRNHQVKKMFESVGLLVDKLSRTRFGTVDLKGLRP
                     GEARRLNKKEISQLHNLANTKK"
     gene            10762..11025
                     /gene="yidD"
                     /locus_tag="VGT87_000118"
     CDS             10762..11025
                     /gene="yidD"
                     /locus_tag="VGT87_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEQ3813010.1"
                     /translation="MMKKLLIVSVKAYQKYISPLSPPSCRYKPTCSAYMLTAIEKHGT
                     KGILMGIARILRCHPFVAGGVDPVPEDFSLMRNKNTSKNAEKA"
     gene            11203..11751
                     /locus_tag="VGT87_000119"
     CDS             11203..11751
                     /locus_tag="VGT87_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEQ3813011.1"
                     /translation="MTTKELINKNDKVKKARNHIVLFQPQIPQNTGNVARTCAATNAP
                     LHIIKPMGFPIDDRKMKRAGLDYWDKLELHFYDHLEQFINQCHGQLHLISKFAVNNYS
                     QATYADGDSHYFLFGREDTGLPEDFMREHAEKALRIPMNDEHVRSLNVSNTVCMVIYE
                     ALRQQGFQGLELKHTYEHDKLK"
     regulatory      11860..11983
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            12062..12625
                     /locus_tag="VGT87_000120"
     CDS             12062..12625
                     /locus_tag="VGT87_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEQ3813012.1"
                     /translation="MSKTHKMIMIGILSAISFLLMLVSFAIIPGAAFLKIEFSIIPVL
                     FGLMIMDLKSAYLILLLRSLLKLFLNNRGVNDFIGLPMNIIAIALFVTAFALVWNRQK
                     TLSQYVFASLLGTGLLTFGMVVLNYTFAIPLYAIFANIDIRAYIGVTKYMMTMVIPFN
                     LVEGLIFAITFYFVYIASKPILERYLH"
     gene            12627..13280
                     /locus_tag="VGT87_000121"
     CDS             12627..13280
                     /locus_tag="VGT87_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEQ3813013.1"
                     /translation="MMTNKQTHFLIASFALLIFVIIGYTVKFFPERLALLDNTIQAEI
                     RGNLPIVLTQFFRGVTVFGNVMTQVLLVIVSVLVLFFMKWKIEALFILSNGAIAAFLI
                     TTLKLFYQRPRPAIEHLVYAGGYSFPSGHAMGSMLIFGSLLIICYQRLHSKLLQFVTS
                     MIFIILILVIGLSRIYLGVHYPSDILAGFVLGFGILQFIYPFYKQKRFEWRFLLKQD"
     gene            13573..14493
                     /locus_tag="VGT87_000122"
     CDS             13573..14493
                     /locus_tag="VGT87_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407327.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEQ3813014.1"
                     /translation="MKKRYQTLNEHYRQLFGAKMFKVPIDAGFDCPNRDGTVAHGGCT
                     FCTVSGSGDAIVAPDAPIKEQFYKEIDFMHRKWPDVNRYLVYFQNFTNTHDTVDVIRD
                     RYEQAINEPGVVGINIGTRPDCLPDDTIAYLAELSERMHVTVELGLQTTYEETSRLIN
                     RAHSYDLYKETVRRLRHYPNINIVSHLINGLPKETHDMMLENVRRCVTDNDIQGIKLH
                     LLHLMTNTRMQRDYHEGRLKLLSQKDYVSIICDQLEIIPKHIVIHRITGDAPRDMLIG
                     PMWSLNKWEVLNAIDKEMERRGSFQGCKVD"
     gene            14532..15113
                     /locus_tag="VGT87_000123"
     CDS             14532..15113
                     /locus_tag="VGT87_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEQ3813015.1"
                     /translation="MLKRPIHLSHDFLAEVVDKSSVVVDATMGNGNDTAFLAQLAKKV
                     YAFDVQEQAIRKTSERLAQLGLSNAELILAGHEAVDQYVTEPVSAAIFNLGYLPSADK
                     SIITLPNTTLQALSKLLTLLMVGGRIAIMVYYGHDGGSLEKDALLDFVKQLDQRKVSA
                     MLYQPLNQVNTPPFLIMLEKLADVYPLSLCQVY"
     gene            15219..16553
                     /locus_tag="VGT87_000124"
     CDS             15219..16553
                     /locus_tag="VGT87_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEQ3813016.1"
                     /translation="MEDPVSQPLVIQFLLLVVLTLLNAFFSASEMALVSLNRSRVEQK
                     AADGDKKYARLLRVLEEPNHFLSTIQVGITFISLLSGASLSASLGKVISGWLGNSATA
                     RTAGTIISLVFLTYVSIVLGELYPKRIAMNLKDKLAIVSAPIIIGLGRLVSPFVWLLS
                     ASTNLLSRLTPMTFDDADEQMTRDEIEYMLSKSEATLDAEEIEMLQGVFSLDEMMARE
                     VMVPRTDAFMIDINDDPLENIQEILKQSFSRIPVYDVDKDKIIGLIHTKRLLESGFRQ
                     GFDQINMRKMLQEPLFVPETIFVDDLLRQLRNTQNQMAILLDEYGGVAGLVTLEDLLE
                     EIVGEIDDETDKAEQFVHEIGDNTYIVVGTMTLNEFNDYFDTELESDDVDTIAGFYLT
                     GIGTIPSQEQKEAYEIDNKDKHLVLINDKVKDGRITKLKLILSNIEQIIEED"
     gene            16631..17422
                     /gene="pflA"
                     /locus_tag="VGT87_000125"
     CDS             16631..17422
                     /gene="pflA"
                     /locus_tag="VGT87_000125"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEQ3813017.1"
                     /translation="MTEKDYGQVTGMVHSTESFGSVDGPGIRFIIFLQGCKLRCQYCH
                     NPDTWEMETNNSKIRTVNDVLKEALQYKHFWGKKGGITVSGGEAMLQINFITALFIEA
                     KKLGIHTTLDTCGFAYRPTPEYHQVLDNLLAVTDLILLDLKEIDEKQHKIVTRQPNKN
                     ILQFARYLSDKQIPVWIRHVLVPGLTDIDDHLTRLGEFVKTLKNVDKFEVLPYHTMGE
                     FKWRELGIPYQLEGVKPPTKERVQNAKNLMQTESYTEYMNRIHQS"
     gene            17553..18488
                     /locus_tag="VGT87_000126"
     CDS             17553..18488
                     /locus_tag="VGT87_000126"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEQ3813018.1"
                     /translation="MSKILVFGHQNPDTDAIASSYAFDYLSQKAFGLDTEVVALGTPN
                     EETAFALDYFGVEAPRVVESAKAQGSEQVILTDHNEFQQSIADIREVEVYGVVDHHRV
                     ANFETANPLYMRVEPVGSASSIVYRMFKENGIEVPKAIAGMLLSGLISDTLLLKSPTT
                     HVSDHLVAEELAELAEVNLEDYGMALLKAGTNLASKSEVELIGIDAKTFELNGNAVRV
                     AQVNTVDIAEVLERQEAIEAAIKDAMAAEGYSDFVLMITDIVNSNSEILAIGANMDKV
                     EAAFNFTLDNNHAFLAGAVSRKKQVVPQLTESFGA"
     gene            18564..19217
                     /locus_tag="VGT87_000127"
     CDS             18564..19217
                     /locus_tag="VGT87_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEQ3813019.1"
                     /translation="MITIFHSDKLTRQPFFQDLINYLDQHDYVILREIKKAFPNVTGI
                     DKAIESYVQAGYIRRENKRYGINLPLVSSDQQLALDTMLFVDTCSAMYENILAVVFET
                     QLTNQTNHVVIKEKTNITRDDLTLANYFYRLKRGEKPSAEQMDLYDLLGDVNQEYALK
                     YMTTFLLKFTRKDLVMQKRPDIFVEALVTLGYLKQVEPTTYQLLMTLDKESLTFIAP"
     gene            complement(19262..20299)
                     /locus_tag="VGT87_000128"
     CDS             complement(19262..20299)
                     /locus_tag="VGT87_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEQ3813020.1"
                     /translation="MLFMVNKKRRPLLKSHRHLLRLYTSLILLLVSLMGLALSLGESH
                     LSFLDLVHVFLGKSSHAISFIVINIRLPRILAAFLGGGSLALSGLLLQRLTRNPLADS
                     GVLGITIGAGISLAIVVSFSFFEQAHISHYLPLFAMLGAVVTTFSVYWLSLTKQGQID
                     PTRLILTGVAVTTMLSSLMVALVGHINRYKVDLVINWLSGQLIGDDWPTLSVIAPLLL
                     CFWLLTYSQAHFLNIMGLADNTAIGLGLPLNRKRRLILVLAAGLGALSVLLVGNISFI
                     GLIAGHFSTYLVGSNHKITIPISILIGMILLLVADTVGRVYLVGSNIQTGILVSLIGA
                     PYFLYLMAKTK"
     gene            complement(20260..21312)
                     /locus_tag="VGT87_000129"
     CDS             complement(20260..21312)
                     /locus_tag="VGT87_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEQ3813021.1"
                     /translation="MKTKHPFLCLERFCLKKTQIITKSHIFWLVFVLLSFSLCVAIYC
                     HLRFGAVALSHQDLNSILFGKQNGHKANVLLAIRLPRLFGATLTGSALAVSGTIMQAI
                     TRNPIAEPGLLGINAGAGLALVLAYAFVPHLHYSLIILLSLLGSSLAATLVFGLSYQS
                     GKGYHQLRLVLAGAMVSILLSALGQGITNYYHLTNAVIGWQAGGLVGVNWQMIGYIAP
                     LIILSLCLAQLLSYHLTVLSLSESQAKALGQKTNLISAVFMILVLILSSAAVAIAGSI
                     SFIGLVIPHLMKHFTPHHYRYLLPLCAVSGASFMVWVDIACRNLNPPYETPLGALVSL
                     IGFPCFLWLIRRGGRY"
     gene            complement(21302..22234)
                     /locus_tag="VGT87_000130"
     CDS             complement(21302..22234)
                     /locus_tag="VGT87_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-hydroxamate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEQ3813022.1"
                     /translation="MKKLTLLLTLCLTTITLIACGNQATNHSNTASKSLSPMPQIAGV
                     TYYGDIPKQPKRVVSLASTYTGYLKKLDMNLVGVTSYDKKNPILAKTVKKAKQVAATD
                     LEAITTLKPDLIVVGSTEENIKQLAEIAPVISIEYRKRDYLQVLSDFGRIFNKEGKAK
                     KWLKDWKTKTAAYEKEVKAVTGDKATFTIMGLYEKDVYLFGKDWGRGGEVIHQAFHYD
                     APEKVKTEVFKQGYLSLSQEVLPDYIVDYVVVAAEDDKTGSALYESKLWQSIPAVKKH
                     HVIKVNANVFYFTDPLSLEYQLETLREAILSSEN"
     gene            complement(22260..23042)
                     /locus_tag="VGT87_000131"
     CDS             complement(22260..23042)
                     /locus_tag="VGT87_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ3813023.1"
                     /translation="MTTISAEDLTIAYEQRTIIDKLSFYIPEGKITTIIGANGCGKSS
                     LLKALTRLLPPKQGVVYLNGQNIATLETKEVAKKLALLPQVQEATNGITVYELVSYGR
                     FPHQSYFGNLSPADKKAIHWAMQATNVMAYADQPVDALSGGQRQRVWLAMALAQGTDT
                     IFLDEPTTYLDLNHQLEILELVKSLNKDAGKTIVMVLHDLNLSARYSDHLIAMKHGKI
                     HYTGTIADVMTSPIIQDIFQIKPVLVDDPIHNCPIVLTYQLQ"
     gene            complement(23288..24733)
                     /locus_tag="VGT87_000132"
     CDS             complement(23288..24733)
                     /locus_tag="VGT87_000132"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEQ3813024.1"
                     /translation="MITIEQLLDILKKDHNFREVLDADGYHYHYQGLSFERLSYDSRQ
                     VDGKTLFFAKGATFKADYLKEAITNGLQLYISEVDYELGIPVVLVTDIKKAMSLIAMA
                     FYGNPQEKLKLLAFTGTKGKTTAAYFAYHMLKESYKPAMFSTMNTTLDGKTFFKSQLT
                     TPESLDLFAMMAECVTNGMTHLIMEVSSQAYLVDRVYGLTFDVGVFLNISPDHIGPIE
                     HPTFEDYFYHKRLLMENSRAVVINSGMDHFSFLADQVADQEHVFYGPLSDNQITTSQA
                     FSFEAKGQLAGHYDIQLIGHFNQENAMAAGLACLRLGASLADIQKGIAKTRVPGRMEV
                     LTMTNHAKVFVDYAHNGDSLEKLLSVVEEHQTGKLMLILGAPGNKGESRRADFGRVIH
                     QHPNLTVILTADDPNFEDPEDISKEIASHIARPVEIISDREQAIQKAMSLCQGAKDAV
                     IIAGKGADAYQIVKGQQVAYAGDLAIAKHYL"
     gene            24821..26455
                     /locus_tag="VGT87_000133"
     CDS             24821..26455
                     /locus_tag="VGT87_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEQ3813025.1"
                     /translation="MSTEKKQLTQEELMVQGAAWSTAGNFISRLLGVLYIIPWYIWMG
                     QYAIQANALFNMGYNVYAYFLLISTTGLNVAIAKQVAKYNSMGQTEHSYQLIRSTLKL
                     MLGLGLIFSVIMYLGSPLFASLSGGDDTLVPIMHSLSLAVFIFPVMSVIRGIFQGHNN
                     IKPYAISQIAEQLIRVIWMLLTTFFIMKLGSKDYLSAVTQSTLAAFVGMIASMAVLGY
                     YLWKLGLLKAICRKPNHHIDSDIKGLLLETLKESIPFIVTGSAIQAFQLIDQWTFVNT
                     MTLFTDYSRSQLLVLFGYFNANPAKITMVLIAVAASIGGVGIALLTENYVKKDMKAAA
                     RLIINNIEMLVMFLLPALTGAIILARPLYSVFYGASEDRAIHLFVAVLFQTLLLALYT
                     LFSPMLQALFENRKAIYYFAYGILIKLVLQIPLIYLLHAYGPLLATTIALVVPIYLMY
                     RRLYQVTHFNRKLLQKRLLLTLIETLLMGLVVFVANWLLGYAFKPTGRLTSLLYLLII
                     GGLGMTVYTALTLLTHQLDKLIGSKASRLRQKLGWH"
     gene            26622..27251
                     /gene="upp"
                     /locus_tag="VGT87_000134"
     CDS             26622..27251
                     /gene="upp"
                     /locus_tag="VGT87_000134"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEQ3813026.1"
                     /translation="MGKCQVISHPLIQHKLSILRRQTTSTKDFRELVNEIAMLMGYEV
                     SRDLPLEDVDIQTPVSKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRNEETLEPVEYLVKLPEDINQRQIFLVDPMLATGGSAILAVDSLKKRGAANIKFVCL
                     VAAPEGVKKLQEAHPDIDIFTAALDDHLNEHGYIVPGLGDAGDRLFGTK"
     gene            27475..28065
                     /locus_tag="VGT87_000135"
     CDS             27475..28065
                     /locus_tag="VGT87_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEQ3813027.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDNTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGTKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRHRLEKILAQNAGK
                     TIKQIHKDAERDYWMSAEETLAYGFIDEIMENNELK"
     gene            28560..28853
                     /locus_tag="VGT87_000136"
     CDS             28560..28853
                     /locus_tag="VGT87_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEQ3813028.1"
                     /translation="MFQKQERIGLVVYLYYNRDARKLSKFGDLYYHSKRSRYLIIYIN
                     KNDLDTKLEEMRRLKCVKDIRPSAFDDIDRQFVGNLHRDETNNHQKGYQPPSY"
     gene            29084..29719
                     /locus_tag="VGT87_000137"
     CDS             29084..29719
                     /locus_tag="VGT87_000137"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEQ3813029.1"
                     /translation="MITGKLITVEGPDGAGKTTVLEQLIPLLKQKVTQEILTTREPGG
                     VAISEYIRELILDINHTTMDPKTELLLYIAARRQHLVEKVLPALEAGQLVFIDRFIDS
                     SVAYQGAGRGLIKADIQWLNEFATDGLEPDLTLYFDVPSEIGLARINANQQREVNRLD
                     LETIEIHQRVRKGYLALAKEHPKRIVTIDATKPLKEVVSVALEHVLALLLA"
     gene            29737..30612
                     /locus_tag="VGT87_000138"
     CDS             29737..30612
                     /locus_tag="VGT87_000138"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEQ3813030.1"
                     /translation="MDLAQKAPNVYQAFQTILKKDRLNHAYLFSGDFANEEMALFLAK
                     VIFCEQKKDQTPCGHCRSCQLIEQGDFADVTVLEPTGQVIKTDVVKEMMANFSQTGYE
                     GKRQVFIIKDCEKMHVNAANSLLKYIEEPQGEAYMFLLTNDDNKVLPTIKSRTQVFQF
                     PKNEAYLFQLAQERGLLRSQADLVAKLAKNVDQLEDLAQNSHFLEVMAQTERFVSIWL
                     KDQLQAYLALNHLVLLATEKEEQELILTLLTLLLAREQSQTPFKQLEAVYQARLMWQS
                     NVNFQNTLEYMVMSE"
     gene            30631..30870
                     /locus_tag="VGT87_000139"
     CDS             30631..30870
                     /locus_tag="VGT87_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tpl protein"
                     /protein_id="HEQ3813031.1"
                     /translation="MGKRSLSKDLPVATKVAAHRGKKAFEENTALATDLMKEVSRLMI
                     QNQLEMTFINSVFPLDQHVVLIPFSAEEHMDFYNC"
     gene            31275..31598
                     /gene="yabA"
                     /locus_tag="VGT87_000140"
     CDS             31275..31598
                     /gene="yabA"
                     /locus_tag="VGT87_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEQ3813032.1"
                     /translation="MNKKELFDAFDGFSQNLMVTLAEIEAMKKQVQSLVEENTILRLE
                     NTKLRERLSHLEHETVAKNPSKQRKDHLEGIYDEGFHICNFFYGQRRENDEECMFCRE
                     LLDRK"
     gene            31603..32466
                     /gene="rsmI"
                     /locus_tag="VGT87_000141"
     CDS             31603..32466
                     /gene="rsmI"
                     /locus_tag="VGT87_000141"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEQ3813033.1"
                     /translation="MQVQKSFKDKKTSGTLYLVPTPIGNLQDMTFRAVATLKEVDFIC
                     AEDTRNTGLLLKHFDIATKQISFHEHNAYEKIPDLIDLLISGRSLAQVSDAGMPSISD
                     PGHDLVKAAIDSDIAVVALPGASAGITALIASGLAPQPHVFYGFLPRKAGQQKAFFED
                     KHHYTETQMFYESPYRIKDTLTNMLACYGDRQVVLVRELTKLFEEYQRGSISEILSYL
                     EETPLKGECLLIVAGAQVDSEIELTADVDLVSLVQKEIQAGAKPNQAIKTIAKAYQVN
                     RQELYQQFHDL"
     gene            32493..32885
                     /locus_tag="VGT87_000142"
     CDS             32493..32885
                     /locus_tag="VGT87_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813034.1"
                     /translation="MTEDQATILILGVYGIILAVFLLSALLCLIANWRLFKRAGYKGW
                     YSLIPFYAGFVKHKITFGEDNKWFYFIGWVIAIYYYYVHFCYVRAFGGSKGLAVFGLF
                     FPGIASLIVAFGKTYTTQECHPVPHLLN"
     gene            complement(32932..33561)
                     /locus_tag="VGT87_000143"
     CDS             complement(32932..33561)
                     /locus_tag="VGT87_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEQ3813035.1"
                     /translation="MIKEFCAENLTLLPTLDAGQVSRVELCDNLAVGGTTPSYGVIKE
                     ACQLLHDKKISVATMIRPRGGDFVYNDLELKAMEEDILKAVEAGSDALVLGLLTTENH
                     LDTDAIEQLLPATQGLPLVFHMAFDHIPTDHQHQALDQLIDYGFVRVLTHGSPEATPI
                     TDNVEQLKSLVTYANKRIEIMIGGGVTAENCQNLSQLTGTAIVHGTKII"
     gene            33860..34216
                     /locus_tag="VGT87_000144"
     CDS             33860..34216
                     /locus_tag="VGT87_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEQ3813036.1"
                     /translation="MITIYQYPKCSTCQRAMAELKQLVSDFEVIDIKANPPKAQDLKH
                     WMETSGYTIKNFFNTSGNSYRELGLKDKIDQLSLDKAAELLATDGMLIKRPILIKDGR
                     VLQVGYRKPYQELDLG"
     gene            complement(34290..35117)
                     /gene="xth"
                     /locus_tag="VGT87_000145"
     CDS             complement(34290..35117)
                     /gene="xth"
                     /locus_tag="VGT87_000145"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEQ3813037.1"
                     /translation="MKLISWNIDSLNAALTGESPRALLSRAVLDTLVAQDADIIAIQE
                     TKLSAKGPTKKHIEALLSYFPNYLHVWRSSVEPARKGYAGTMFLYKNTLNPVITFPEI
                     GAPTTMDAEGRIITLEFEDFFVTQVYTPNAGDGLRRLDDRQIWDHKYADYLTELDAQK
                     PVLATGDYNVAHKEIDLANPSSNRRSPGFTDEERQGFTNLLARGFTDTFRHLHGDIPH
                     VYTWWAQRSKTSKINNTGWRIDYWLASNRLVDKVKRSEMISSGERQDHTPILLDIDL"
     gene            35351..36532
                     /gene="lctO"
                     /locus_tag="VGT87_000146"
     CDS             35351..36532
                     /gene="lctO"
                     /locus_tag="VGT87_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEQ3813038.1"
                     /translation="MAQKTVITEETTDFVMDFKTSSAEGNVDFINVFDLEKMAQQVIP
                     KGAFGYIASGAGDTFTLHENIRSFNHKLIVPHSLKGVENPSTEITFDGDHLTSPLILA
                     PVAAHKLANEQGEVASAKGLKEFGSIYTTSSYSTTDLPEISAALGGTPHWFQFYYSKD
                     DGINRNIMDRVKAQGCKAIVLTADATVGGNREVDRRNGFVFPVGMPIVQEYLPDGAGK
                     TMDYVYKSAKQALTSKDIEYIATYSGLPVYVKGPQCAEDTLRALDAGASGIWVTNHGG
                     RQLDGGPAAFDSLQEVAEAVDQKVPIVFDSGIRRGQHIFKALASGADLVALGRPAIYG
                     LAMGGSTGTRQVFEKLNDELKMVMQLAGTQTIQDVKAFNLRHNPYDSSIPFDQNALRL
                     D"
     gene            36796..41739
                     /gene="scpC"
                     /locus_tag="VGT87_000147"
     CDS             36796..41739
                     /gene="scpC"
                     /locus_tag="VGT87_000147"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CXC chemokine-degrading serine protease SpyCEP"
                     /protein_id="HEQ3813039.1"
                     /translation="MEKKQRFSLRKYKSGTFSVLIGSVFLMMTTTVAADELTTTSEPT
                     ITNHAQQQAQHLTNTELSSAESKPQDTSQITPKTNREKEQSQDLVSEPTTTELADTDS
                     APMANTGSDATQKSASLPPVNTDVHDWVKTKGAWDKGYKGQGKVVAVIDTGIDPAHQS
                     MRISDVSTAKVKSKEDMLARQKAAGINYGSWINDKVVFAHNYVENSDNIKENQFEDFD
                     EDWENFEFDAEAEPKAIKKHKIYRPQSTQAPKETVIKTEETDGSHDIDWTQTDDDTKY
                     ESHGMHVTGIVAGNSKEAAATGERFLGIAPEAQVMFMRVFANDVMGSAESLFIKAIED
                     AVALGADVINLSLGTANGAQLSGSKPLMEAIEKAKKAGVSVVVAAGNERVYGSDHDDP
                     LATNPDYGLVGSPSTGRTPTSVAAINSKWVIQRLMTVKELENRADLNHGKAIYSESVD
                     FKNIKDSLGYDKSHQFAYVKESTDAGYNAQDVKGKIALIERDPNKTYDEMIALAKKHG
                     ALGVLIFNNKPGQSNRSMRLTSNGMGIPSAFISHEFGKAMSQLNTNGTGSLVFDSVVS
                     KAPSQKGNEMNHFSNWGLTSDGYLKPDITAPGGDIYSTYNDNHYGSQTGTSMASPQIA
                     GASLLVKQYLEKTQPNLPKEKIADIVKNLLMSNAQIHVNPETKTTTSPRQQGAGLLNI
                     DGAVTSGLYVTGKDNYGSISLGNITDTMTFDVTVHNLSNKAKTLRYDTELLTDHVDPQ
                     KGRFTLTSRSLKTYQGGEVTVPANGKVTVTVTMDVSQFTKELTKQMPNGYYLEGFVRF
                     RDSQDDQLNRVNIPFVGFKGQFENLAVAEESIYRLKSQGKTGFYFDESGPKDDIYVGK
                     HFTGLVTLGSETNVSTKTISDNGLHTLGTFKNADGKFILEKNAQGNPVLAISPNGDNN
                     QDFAAFKGVFLRKYQGLKASVYHASDKEHKNPLWVSPESFKGDKNFNSDIRFAKSTTL
                     LGTAFSGKSLTGAELPDGYYHYVVSYYPDVVGAKRQEMTFDMILDRQKPVLPQATFDP
                     ETNRFKPEPLKDRGLAGVRKDSVFYLERKDNKPYTVTINDSYKYVSVEDNKTFVERQA
                     DGSFILPLDKAKLGDFYYMVEDFAGNVAIAKLGDHLPQTLGKTPIKLKLTDGNYQTKE
                     TLKDNLEMTQSDTGLVTNQAQLAVVHRNQPQSQLTKMNQDFFISPNEDGNKDFVAFKG
                     LKNNVYNDLTVNVYAKDDHQKQTPIWSSQAGASASAIESTAWYGITARGSKVMPGDYQ
                     YVVTYRDEHGKEHQKQYTISVNDKKPMITQGRFDTINGVDHFTPDKTKALGSSGIVRE
                     EVFYLAKKNGRKFDVTEGKDGITVSDNKMYIPKNPDGSYTISKRDGVTLSDYYYLVED
                     RAGNVSFATLRDLKAVGKDKAVVNFGLDLPVPEDKQIVNFTYLVRDADGKPIENLEYY
                     NNSGNSLILPYGKYTVELLTYDTNAAKLESDKIVSFTLSADNNFQQVTFKMTMLATSQ
                     ITAHFDHLLPEGSRVSLKTAQGQLIPLEQSLYVPKAYGKTVQEGTYEVVVSLPKGYRI
                     EGNTKVNTLPNEVHELSLRLVKVGDASDSTGDHKVMSKNNSQALTAFATPTKTTTSAT
                     AKALPSAGEKMGLKLRIVGLVLLGLTCVFSRKKSTKD"
BASE COUNT        13347 a         7679 c         8622 g        12227 t
ORIGIN      
        1 gaaaaagaac aacttgaaaa agaacttgaa gaattaaaac ttgtgcgtcg tccagaaata
       61 gtggagcgta ttaaaattgc acgttcgtat ggagatcttt cagagaactc tgaatacgat
      121 gccgcaaaag atgagcaagc ttttgttgaa ggtcaaattt caacgcttga aactaagatt
      181 cgctatgctg aaatcattga cagtgatgct gttgcaaaag atgaagtcgc tatcggaaaa
      241 acagttatcg ttcaagaagt tggaacaaca gataaagata cctatcatat tgtaggtgct
      301 gctggagcag atatcttctc aggaaaaatt tcaaatgaaa gcccgattgc tcaagctttg
      361 attggtaaaa aaacaggtga caaggttaga attgaatcac cagcagctac ttatgatgtt
      421 gaaatcatca gcgttgaaaa gacaaattaa tcagatgaag ccattatttg cctaaccact
      481 aagtgttttg gttgctaatg gcttttctag tatcttcaaa tgaggttgac tgttaggaag
      541 gtcaagtatt aatcgcattg attatgagcc aagaggacta tagatcaaag atcgttttcg
      601 atggttattt aatgccaaaa gaaaagacca gcaccttaat gtactgaccc caaaaagttg
      661 gacactatat tttaaggaag tgtatgatct aaggaaactg acactaatga ttgaccgttt
      721 tgcaagactt tattaatgat ttcttgcttt attttaaatg aataagtctg atacgtttcc
      781 ttccttaaca ctatcgactc catactgacc catcagtcaa acggtatatc tcaaacgact
      841 acggtttact ccaaattgat tggctagttg aggccaagaa acacctgatt ttttttaatg
      901 ctaaatttct agcttatctt catagcttaa tttcatacaa aaacacccca atcgttagat
      961 ttttttgata tgtccccttt cactagatga gtccagtaaa agggggcata tcgttttttg
     1021 tctaactttt ggggtgcagt tcataattgt gctaggtttt ttgtctttct taacgctttc
     1081 gctgttttcc agcattacga ttggatttta tttttttagg caagttagcc tgttccatgt
     1141 tttgtgaagg cgtaacgtcc ttacgagaag aagttgattg gtatgcttta ggcgggtttt
     1201 tagcgtattc ttctttaatt tgcttatgca atcgaggttt taagaggtag gctgtgatta
     1261 actgttgaat aatactaaag aaaccaccaa ctagccagta aagaccaaca ccagcaggga
     1321 gtgagaagga catgaagatc atcatgatag gcatggtgta catcatcgtc ttcatttgct
     1381 cacgttgttc ttctgaaacg gccatcattg ataaccatga ttggaagaag taaagagcag
     1441 cgataattgc tgttaacact aaactacggc taccaagatc aatacccata aaggtgcttg
     1501 ttgatactcc tttagtgtat tgagcggcaa aatacatggc agaaaagaat ggcatctgga
     1561 tgagaagagg tagacatcca atacctccaa gaggattaat cccatgagca cgctgagcag
     1621 ccattaattc tgtttgggca gccatttttt cttcctgact acttgcctgt ttgatacgtt
     1681 tgttgattgg ttcaaaaaca ggtttcaaga aggccatttt ctcagattga taacttgctt
     1741 tccaagattg atatagacct agtggtaaga taagtgttct aacaataatg gttacgatga
     1801 tgatagccag tccataacct agtccagcat tgtttgcaaa gtaatcgata aagtatgaca
     1861 tgggttttcc aagaaattcc caaatcatac ctttgggatt tccgtgagcg tctcttccca
     1921 cacagcctgt caaggtgaat agaattgaca gtgctagtcc tgaaaagagg atgcgattta
     1981 atgttagttt caatgtgttt atcctttaat tttaatatac ttatctattt tacagttttt
     2041 ttcagaaatt cacaatagtt ctgaaggccg atttttactt gcctatttga aagtcctgaa
     2101 agtcttcaaa attagccatg gtgacatcca cataagtgac ttttgcccat ttggatggtc
     2161 ctttacgaac ttcttgaata aatgtggcta ttttatctga atcttttgat tgggccagga
     2221 tttcaacggt cccatcgttg ttattccaga cacgaccata aatatctcct atatctagag
     2281 ctaaagtatg ggtagcgtat cgaaaaccaa caccttgtac acgtccagaa acaattaaac
     2341 gtactttttg cataagaagg gcctccttgc ttttattata gcacatactt agcaggaaat
     2401 ctctttagct aattgtctac ttcctgtcac ttttactaaa gacaagaata caaaaatgag
     2461 atagttagta gtactaacag taaaagaggt ttgtctattt taaaaatagt atcaaaaaaa
     2521 ggtcttttag gtttaaaaca ttgaactgcc cccaaattga ccatctaaga agttgatgct
     2581 ttcaaatgct gtcgcgtatg gtataatagg cgcatggtta ttacatcaaa aacaaatcaa
     2641 ctaattaagc aaacaaaaaa attattgcaa aaaaagcatc gaaaacagtc ctatttgatt
     2701 gagggctggc acctttttga agaagcccaa aaatcaggac aagtttttcg ccacatcttt
     2761 gttttagagg aaatggtgga gcgtctagca ggtgagcaag aactcgttat tgttagccca
     2821 caagtgctca aggagttgac cgactctcct agtccacaag gcattgttgc tgaggtggag
     2881 atacctaagt tagcctttcc atcagattac aaagggaaat atttggtttt agaggacgtc
     2941 caagacccag gaaatcttgg gaccatcatt cgtacagcag acgctgcacg gtttgatggg
     3001 gtctttcttt ctgaaaaatc agcagatatt tacaaccaaa aaaccctacg ttccatgcaa
     3061 ggtagccatt ttcatttacc aatttggcga acagatgtct atcagctttg tcgagagctc
     3121 caagagtata aaactcccat cctagcaaca actctgtcaa agaaatctgt tgactataaa
     3181 agcctcacac atcatgaaag gttggctttg gtgttgggaa atgaaggcca gggcattagt
     3241 gctgaaatgg cagccctagc agaccaactg gttcatatta caatgcctgg tcaggcggaa
     3301 agtttgaatg ttgccgtagc agcaggaatc ctcattttca gcttgattta agctaatatg
     3361 atataataac caagtgaggt aattatgtta tgcattatac agaagataaa gaatatatgg
     3421 aacacgtcgg tcatttgatt gctcatcctc gctttcaaaa attgagtcat attgttcagc
     3481 accaacattc aacgcgtctt gaacattcta tcaatgtttc ttattccagt tataaattag
     3541 ccaagcgatt tggttgggat gctaaaagta cagccagagg gggcttactt catgatttct
     3601 tttattatga ttggcgcgtg accaagttta ataagggtca tgcctgggtt catcctcgta
     3661 ttgctgtacg aaatgcgaaa aagttaacag aactcaataa aaaagaagag gatatcattc
     3721 taaaacacat gtggggagcg accattgctt ttccgcgtta caaggaaagt tatattgtga
     3781 caatggtaga taaatactgg gctgtcaagg aagcagtgac gcctttgaga caaaaatggt
     3841 ctaatcgacg gtttcttcgc cgcaagaccc tccaaagtca taatcgttaa gtaaaggagt
     3901 tattatgaac gatcacgtta tttatacaca atcagatgtg ggattaaatc agttttttgc
     3961 taaaatttat agtctagtgg gaatgggagt gggtttgtct gcttttgttt cttatttgat
     4021 gctttatcca tttagagaaa atttaatctc tattcttgtt aatcagccga tgatttacta
     4081 tggtgctgcg attattgaac ttattttagt ttttgtagcc agtggtgcag cacgaaaaaa
     4141 cacaccagct gcattgccca tttttcttat ctattttgcg ctaaatggct ttaccctaag
     4201 ctttattatt gtggcatacg ctcaaacgac agtttttcaa gcatttcttt cttcagcagc
     4261 tgtgtttttt gccatgtcaa tcattggtgt caagactaaa cgggatatgt caggtcttag
     4321 aaaggctatg tttgctgctt tgattggtgt tgttgtggct agtctgatta atctttttat
     4381 tggtagtggg atgatgagtt atgtcattag tgtgatttcc gtgctaattt tctcaggtct
     4441 catcgcatca gataatcaaa tgattaagcg cgtttatcaa gcaacaaatg gccaggttgg
     4501 tgatggctgg gctgttgcta tggccttaag cttgtattta gattttatta acttatttat
     4561 cagccttctt cgtatttttg gaagaaatga ttaacattaa aaagtgagta taactactca
     4621 ctttttaatg ttaacaggat aagtaaactg taaggaagaa attcgagata gatataagat
     4681 gaggctgcta aaagacctag tgttaatttg taaactcttt ctttttttgg tatactgtta
     4741 aggattaaat acgaggagaa tatagataga tatgtcaaca gctatttgga ttttattact
     4801 tattgttgcc cttggggtag gtgtttttgg tgggattttt attgcccgta aacaaattga
     4861 aaaagaaatt ggtgaacacc cacgtttaac accagaagct atccgtgaaa tgatgagcca
     4921 gatggggcaa aaaccaagtg aagcaaaaat ccaacaaact tatcgtaata tcatcaaaca
     4981 atcaaaagca gccgtatcca aaggcaaaaa ataaaagcta attgttatag gggaaacctt
     5041 tgtgatagtt agaagtgaac agtctttgat ctcaatgttc agtagcactt gtcttatcag
     5101 ttagatttgt caactgttca gggtttaaaa agaaactcag ttggctattt ccaactgagt
     5161 ttttcatatc gtcagtaagg aagtaaacat aatggataca agaccaattg ggtttttaga
     5221 ttctggtgtg ggaggtctga ccgtcgtttg tgaattgatt cgtcagctcc ctcatgagaa
     5281 gattgtatat attggtgatt cagcaagagc cccctatggt cctagaccca aaaaacaaat
     5341 taaggagtat acttgggagc tagtcaattt tttgctgact caaaatgtta aaatgattgt
     5401 ttttgcatgc aataccgcaa cagcggtggc ttgggaagaa gtaaaagcag ctttagatat
     5461 tcctgtttta ggggttgtct taccgggggc aagcgcagct attaaatcaa cgacaaaagg
     5521 ccaggttggg gtcatcggaa ccccaatgac agtggcttca gacatttatc gcaaaaaaat
     5581 ccagctatta gcaccatcta ttcaagtaag gagtcttgct tgcccgaagt ttgtaccgat
     5641 tgtggaatca aatgagatgt gttcgagtat agctaaaaaa atagtttatg acagtctatc
     5701 accattagtc ggtaaaatag atacccttgt actaggatgt actcactatc ccttgttacg
     5761 accaattatc caaaatgtta tgggaccatc tgttaagctg attgacagtg gagcagaatg
     5821 cgtccgagat atctctgtct tattaaacta ttttgacatt aacggtaatt atcatcaaaa
     5881 agctgtcgaa caccgctttt ttacaacagc aaatcctgag atttttcaag aaattgcctc
     5941 gatttggctg aaacaaaaaa taaatgtgga gcatgtcacc ttatgagtga aaaaatttac
     6001 gaatacaaag atgaaaataa ctggtttatc ggaaagatga ctggtcataa tttaatatcg
     6061 ggctggggag tcaaacacac gacaatcaaa aaaatagatg accttttaga cggtattgcc
     6121 gcaacgttag attgggaaaa tcctaaaggt tacgatgttt cggtggtgcg ttatcagtct
     6181 cctttatctc tcatcacctt tattatcgac atgataaatc aagagaccca gcgtaaggtt
     6241 aaggtaactc cccatgcagg cacgatttta ttaatggaaa atgcgaaact gcttgcagtt
     6301 tatctcccag aaggtggagt ttcaacggct acattttttg cgactagtga acagagattt
     6361 ggggatactg ttttaatcgc tacacgaaac gagggaaaaa ccaaagagtt ccgcaatctg
     6421 tttggccagc taggttatcg tgtggaaaat ctcaatgatt atccagaatt gccagaagta
     6481 gcagaaactg gtactacttt tgaagaaaat gctcgcttga aagcagaaac gatttcacgc
     6541 ctcacaggaa aaatggtatt agcagacgat tcaggactaa aagttgatgc cttgggtggt
     6601 ttacctgggg tttggtcagc tcgtttctca ggaccagatg ccacagatgc caagaataat
     6661 accaaactct tacacgaatt agctatggtg tttgaccaaa agaagcgctc agctcaattt
     6721 catacgactt tggtagtggc tgctcccaat aaagatagtt tggttgtgga agctgagtgg
     6781 ccaggctata ttgctacgca gcctaaggga gaaaatggtt ttgggtatga ccctgtcttt
     6841 atcgtcggag agactgggcg tcatgctgca gaattagagg ctgaccaaaa gaatcagttg
     6901 tcgcatagag gtcaagcagt tcgaaaatta atggaggtct ttccagcatg gcaagcgaaa
     6961 caatcatagt aatgagtgac tctcatggtg accgagatat cgttcaagcc attaaagaca
     7021 agtatcttgg tcaagttgat gctattttcc ataatgggga ttctgaatta aacagctcag
     7081 atcctatttg ggctggtatt tatgtcgttg gcggtaattg tgactatgat acaggttacc
     7141 ctgatcgttt agtaactcaa ttaggcacag ttacgattgc ccagacacat ggtcacttgt
     7201 accatattaa tttcacttgg gataaactgg actactttgc tcaagaagta gcagcagata
     7261 tttgtctata tggacaccta caccgtccag ctgcttggca agttgggcaa accctcttta
     7321 tgaatcccgg atcagtaaca caacctcgcg gagaaattaa cgagaaatta tatgctcgtg
     7381 ttgaactaac tgatactcaa attaaagttg attactttac aagagatcat aaactttacc
     7441 catcactgtc taaggaattt aaacgatgat cgcaaaagaa tttgaaacgt ttttaatgag
     7501 tcatttagat aattatctta tcccggaaca agacttggct atttttatcg acacacataa
     7561 tgcagaccat gtgatgttac tattggtgag taatggtttt tctagagttc ctgttatcac
     7621 acgggagaaa aaatatgtgg gaaccattag catctcagat attatgatgt accaatctaa
     7681 aaggcagctg acagattggg aaatgagtca aacagatatt ggcgagatgg ttaataccaa
     7741 aattgaaacc attagcatta cttctagcct aacagaaatt atgcacaagt taattgattt
     7801 cccattctta ccggttgtag atagagcgaa tcgctttgtt gggattatta cgcgaaaatc
     7861 tatattaaag gctgtcaaca gtcttttaca tgattttaca gatgattaca cgattataaa
     7921 aaaatgaaga gttatattga accttttatt gctagcaagg ctttatctca aaatagtcaa
     7981 aaagcttatc gatacgattt gcaacagttt tgtcaactgg ttggtgaacg tgtcaatcaa
     8041 gacaaactat tactttatca aaatagcata gctaacctaa gtttatctgc aaaaaaacgc
     8101 aaattatcaa cagctaatca attcttatat tacttgtatc aaataaagta tttaaatagc
     8161 tattttcgat taacagatac catgaaagtg atgcggactg agaagcagca agctgctatt
     8221 attaatactg atattttcta tcaaaaaacc ccttttgtgt gggggcaatt aatcagctta
     8281 ttaatattgg agctgggttt aactccgagt gaagtagctg gtattgaagt agctaacttg
     8341 gatttaaatt ttcaaatgtt gacgctaaag actaaaaaag gtgtgcgtgt attacctttg
     8401 tcgcaaatac taatcccttt tttagaacaa caactcgttg gcaaagaagt ctatcttttt
     8461 gaacatcgag gcatcccttt ttcaagacaa tggtttttta atcacctaaa aaccttcgtt
     8521 cgaagtattg gttatgaagg attaactgct caaaaattac gagagcaatt tattttgaaa
     8581 gaaaaactag ctggtaaatc gataatagaa cttagcgata ttcttgggct gaaaagccct
     8641 gtgaccttag aaaaatacta caaatcataa tggatataaa acttaaagat tttgaaggtc
     8701 ctcttgattt actcttgcac ttggtatcac agtacaaagt tgacatctac gaggttccta
     8761 ttgttgaggt tattgagcag taccttaatt atattgaaac cttacaggta atgaaacttg
     8821 aagtggctgg tgactacatg ctgatggcaa gtcagctcat gttaatcaaa agtagaaggc
     8881 tattaccaaa ggtggtagaa cacatcgaag aagaagattt agaacaagat ctgttagaaa
     8941 agatcgaaga atatagtcgc tttaaagcag ttagtcaagc gttagcgaag caacatgacc
     9001 aaagggcaaa atggtattca aaacctaaac aagaattaat ttttgaagat gctatcttac
     9061 aagaagataa aacggtgatg gatctatttc tagctttttc aaacataatg gcagctaaaa
     9121 gagcagtttt aaaaaccaac catacagtca tcgagcctga tgattataag attgaagaca
     9181 tgatggcttc tattaaacag cggctggaaa aagaaaacgt tattagctta tcagcgattt
     9241 ttgaggaatg ccaaacatta aatgaagtta tcagtatatt tttagctagt ctagaattga
     9301 ttaagcttca cgtggtattt gtagagcagc tctctaattt tggggctatc atactacgaa
     9361 aggagaagaa atgacgtatt tgtcacagat agaagcgctg ctatttgttg caggtgaaga
     9421 aggattaagt ttgcgacatc tagcgtctat gttatcatta acgccaacag ctcttcaaca
     9481 gcaattagaa aaactctctc aaaaatatga aaaagatcaa cattctagtc tttgcttgat
     9541 agaaacagct aatacctaca ggctagtaac taaagaaggt tttgcagaac tcttgcgtgc
     9601 ttatgccaaa acaccaatga accaaagttt atctcgtgcg agtttagagg tcttgtctat
     9661 tgttgcatac aaacaaccta ttacacgaat agaaattgat gacattcgag gggtaaactc
     9721 cagtggagct ttgagtaaat tattggcatt tgatctcatt agagaagctg gcaaaaaaga
     9781 tgtggttggg cgtcctcatt tgtatgcgac aacagattat tttctggact atatgggaat
     9841 taatcattta gacgaattga ttgaagtatc tgccgttgaa ccggcagatg aagagattgc
     9901 tctttttagg acacaagact gagttttgct tgtcatcaaa tagcaaatga cgtttacaaa
     9961 aagatttgtt ttaaggcgtt ttagttgaaa aaagccttaa actagggaca acaaaaagtt
    10021 gctcaaaaga aagaagaata taatgcgaat taacaaatac attgcccatg cgggtattgc
    10081 cagtcgtcgt aaggctgaag aattgattaa gcaaggctta gtaaccttaa atggtcaggt
    10141 tattacagat ttagcaacca ctgtaaaatc gggagacgtg gttgaaatag aaggaagtcc
    10201 tatttacaac gaggaaaagg tttactattt gcttaataaa cctcgtggcg ctatctcaag
    10261 tgtgtcagat gacaaaggcc gtaaaacggt actgggttta ctacctcagg ttaaagaacg
    10321 catataccct gttggacgat tggattggga cacatcaggg gtccttattt taacgaatga
    10381 tggtgatttt acagatacta tgattcatcc tagaaatgaa atagacaagg tttatctggc
    10441 gcgtgtcaaa ggaattgcaa cgaaagaaaa tcttcgtccc ttaactcgag gaattgttat
    10501 tgatggtaaa aagaccaaac cagctcgcta caatattgtt cgtgtagaag ctgataagaa
    10561 tcgatcgatc gtagaattga ctatccatga agggcgaaac catcaggtta aaaaaatgtt
    10621 tgagtctgtg gggcttttgg ttgataaatt atctcgtaca cgttttggga ccgtcgactt
    10681 aaagggacta agaccaggag aagcaagacg cttaaataaa aaagaaataa gtcaactgca
    10741 taacttagct aatacgaaaa aatgatgaaa aaactattaa tagtatctgt taaagcttat
    10801 caaaaataca tttctccttt atctcctcct agttgtcgct acaagccaac ctgttcagct
    10861 tatatgttaa ctgctattga aaaacatggt actaagggaa tattaatggg gatagcaaga
    10921 atattaagat gtcacccttt tgttgctggt ggtgtcgatc ctgttccgga agattttagc
    10981 ctaatgagaa ataaaaacac gtctaaaaac gctgaaaagg catgacttta tcaaaaaatg
    11041 gcttgacatt gatagctgaa gtgttataat ggaaaacaag cattcgttgg tttaaatcaa
    11101 acctgttatg atttaagtta gcgagtcacc atcaaccaca ttgtttgctg agcatgactc
    11161 cgagcagtgt ggttttttaa attaaaaaga gtaaatgaga ttatgacgac aaaagaactt
    11221 atcaacaaaa acgacaaggt aaaaaaggct cgcaatcata ttgtcctgtt tcaaccccaa
    11281 attccacaaa atactggtaa tgttgctaga acatgtgctg caactaatgc acctttacac
    11341 attattaaac ccatgggatt tcctattgat gaccgcaaaa tgaagcgtgc cggcttagat
    11401 tactgggaca agttagaact gcatttttat gatcatttag aacagtttat caatcaatgt
    11461 cacggacagc ttcacctgat tagcaagttt gcagttaata attattctca agctacttat
    11521 gctgatggcg attcccatta tttcttgttt ggacgagaag atacaggcct gccagaagat
    11581 tttatgcgag aacatgctga aaaagcactt cgtattccga tgaatgatga gcatgtacgg
    11641 agtttaaatg tctcaaatac ggtttgtatg gtgatttatg aagcccttag acaacagggc
    11701 tttcaagggt tagagttaaa gcatacttac gaacacgata aactaaaata atggactttt
    11761 taaaagcgtc agtttttggc aacatgcgtt gtcagtagct gacgctttta aaaatttagt
    11821 caaagctatt gctatctagg tttaaatctg ctatgctaga agtgtcttca gggcagggtg
    11881 tgattcccga ccggcggtga ctagtaacta gaagtccgcg agcgcaagct gatgtggtgt
    11941 aactccacaa ccgacagtat agtctggatg agagaagacc gggctttttg tgtaggctta
    12001 ttttagctac acaataacga cggaacttct ttcaatttga aaaaattgga ggaatttttt
    12061 aatgtcaaaa acacataaaa tgattatgat tggtatactt tctgcgatat catttctatt
    12121 gatgctagtt agctttgcca ttattccagg cgcagctttt ttaaaaattg aatttagtat
    12181 tattcctgtc ctatttggtt taatgattat ggacttaaag agtgcttact tgattttgtt
    12241 attgaggtcc ttattgaaac tctttttgaa caatcgtggg gttaatgatt ttattggtct
    12301 cccaatgaat attatcgcta tcgctctatt tgtgacggct tttgccttag tttggaaccg
    12361 tcaaaaaacg cttagtcaat atgtatttgc tagcttacta ggcacagggt tattaacatt
    12421 tggcatggtt gttctcaatt atacttttgc cattccttta tatgctatat ttgcaaatat
    12481 tgatatcaga gcttatattg gtgtcactaa gtatatgatg actatggtta ttccgtttaa
    12541 tcttgttgaa gggttgatat ttgcaattac cttttatttt gtgtatattg caagtaaacc
    12601 aattttagaa agatacttac actaatatga tgacaaacaa acaaactcac tttttaatag
    12661 cttcttttgc attactaatc tttgttataa taggctatac ggtcaaattt tttcctgaac
    12721 gtttggcact tctagataat actattcaag cagagattag aggcaacctt cctatagtgc
    12781 tgacgcaatt ctttaggggt gttactgtat ttggaaatgt catgacacaa gttttgttag
    12841 ttattgtgtc ggtcctagtt ttattcttta tgaagtggaa aattgaagcc ttgtttatct
    12901 tatcaaatgg tgccattgca gcatttttga ttacgactct caagctcttt taccaaagac
    12961 caagacctgc tattgagcat ttggtttatg ctggtggcta ttcgtttccc agtggccatg
    13021 cgatgggtag catgctgatt tttggtagcc tattgattat ttgttatcaa aggctacatt
    13081 caaaactatt acaatttgtg actagcatga tctttataat actaatttta gtaattgggt
    13141 tatctcgtat ttatctaggg gtacactacc caagtgatat tttagcaggc tttgtattag
    13201 gatttgggat tttgcagttt atttatcctt tttataagca aaaacgattt gaatggcgat
    13261 ttcttttgaa acaagactaa tttttcttga gaagacatag ggcttattta aatcatttag
    13321 aataacattg gcaagcatca ccccttgatt gccagtgtta ttttagtttt agttagatga
    13381 tgtgagctga tgattagtaa atacgtttag ataaaaaatg attaaagtgc tatgtaacgt
    13441 cagaaaagac gtgatttttg ttgatctgta atcattaagt aagctgatgt agaagtagtg
    13501 aattctaatg actgagctat cttttgcata gatgactatc ttgctagtaa tgatttgtgc
    13561 tacaatagaa acatgaaaaa aagatatcag acattaaacg agcattaccg tcaactattt
    13621 ggcgcaaaaa tgtttaaagt tccgattgat gccggctttg attgtcccaa tcgtgatgga
    13681 acggtagctc atggaggatg tactttttgc acagtatcag gttctggaga tgctattgta
    13741 gcaccagatg ctccaattaa agagcaattt tataaagaaa ttgattttat gcaccgtaaa
    13801 tggcctgatg tcaatcgcta tttggtttat tttcaaaact ttaccaatac tcatgataca
    13861 gttgatgtga ttcgggaccg ttacgaacaa gctattaacg aacctggagt tgttggtatt
    13921 aatattggca cacggcctga ctgtctacca gatgatacca ttgcttactt agcagaatta
    13981 tcagaacgca tgcatgtgac agtggagcta ggcttacaga caacttatga agaaacttct
    14041 aggctaatta acagagcaca ttcttatgat ttgtataaag aaacggtgag gcgactaagg
    14101 cattacccca acattaatat tgtctcgcac ttgattaatg gattgccaaa agaaacccat
    14161 gacatgatgc ttgaaaatgt gagacgctgt gtaacagaca atgacattca aggaattaaa
    14221 ttgcatttac tgcatttgat gaccaatact agaatgcaaa gagattacca tgaaggacgc
    14281 ttaaagttgt tgagtcaaaa ggactatgtt tcgattattt gtgaccagct agagattatc
    14341 cctaagcata ttgtgattca ccgtattaca ggagatgccc cacgagatat gttgattgga
    14401 cccatgtgga gtctaaataa gtgggaagta ttgaatgcca ttgataaaga aatggaaaga
    14461 cgaggaagct ttcagggctg taaagttgac taatttgtct cattttgacg gatttggata
    14521 ggagaaaagt gatgctaaaa cgccccattc atctctcgca tgatttttta gcggaggttg
    14581 tggataagtc tagtgtggtt gttgatgcca cgatgggtaa tggaaatgat actgcctttt
    14641 tggcacaatt agctaaaaag gtttatgctt ttgacgtgca agaacaagct attagaaaaa
    14701 caagtgagcg gttagcgcag ctaggattat cgaacgctga gctgatttta gcgggtcatg
    14761 aagcagttga tcagtatgtg acggagccgg tgagtgcagc gatttttaac cttggttatt
    14821 tgcccagcgc agataaatct attattacct taccaaatac cacactccaa gctttatcaa
    14881 aactactaac gttattaatg gtaggtgggc gtattgctat tatggtctat tatgggcatg
    14941 atggaggcag tttggaaaaa gatgctttat tagattttgt caagcaatta gaccaaagaa
    15001 aggtttctgc aatgctttat cagcctctta atcaggtgaa taccccacct tttttaatca
    15061 tgttggagaa gttagcagac gtttatccct tatccctgtg tcaagtctac tagaagttct
    15121 gcgattattt cggtggaaaa agaagaaatc ccataggctt tggctataaa tgtgatataa
    15181 tgaagtcact atattatttt tttaggagtt acattaacat ggaagaccct gtgagtcagc
    15241 ccttagtgat tcaattttta ttgttagttg ttttaacctt gttaaatgct tttttttcag
    15301 ccagtgaaat ggccttagtt tctctcaatc gttctcgggt ggaacaaaaa gcagcagacg
    15361 gtgataaaaa atacgctcgt ttgttgcggg ttttagagga acctaatcat tttttatcaa
    15421 cgattcaagt tgggattacc tttattagtt tactatcagg agcaagttta tcagcttctt
    15481 tgggtaaggt gatctcaggt tggctaggta attcagcgac tgcaaggaca gctggtacta
    15541 tcatctcctt ggttttcttg acttatgtct ctattgtttt aggagaattg tatccaaaac
    15601 ggattgccat gaacctcaaa gacaagttgg cgatcgtttc agcccctatt atcattgggt
    15661 tggggagact ggttagtcct tttgtgtggc tcttatctgc ttctactaat ttgctgagcc
    15721 gacttacccc aatgaccttt gatgatgcag atgagcaaat gacacgtgat gaaatcgagt
    15781 atatgttatc aaaaagtgag gcgacccttg atgctgaaga aattgagatg ttgcaaggag
    15841 ttttctcact tgatgaaatg atggcgcgtg aagtcatggt cccaaggacc gatgctttca
    15901 tgattgacat taacgatgat ccgcttgaaa atattcagga aatcttaaaa caaagttttt
    15961 cacgcattcc tgtttatgat gtggataaag ataaaattat cggtctcatc cacactaagc
    16021 gtctcttgga gtcaggtttc cgccagggat ttgatcagat taacatgcga aaaatgttac
    16081 aagaacctct ttttgttccc gaaaccattt ttgtagatga tctcttacgc cagctgcgca
    16141 atacccaaaa tcagatggct attttgctag atgaatatgg tggtgtggca ggacttgtga
    16201 ctttggaaga cttgcttgaa gaaatcgtcg gtgaaatcga tgatgaaacc gataaagcag
    16261 aacaatttgt tcatgagatt ggagacaata cctatattgt tgttggtacc atgactttaa
    16321 atgagtttaa tgactatttt gataccgaac tagaatcaga tgatgtagat accattgctg
    16381 gtttttattt gacaggtatc ggaaccattc caagccagga gcaaaaagaa gcctacgaaa
    16441 tagataacaa agacaaacat ttagttctaa tcaacgataa agtcaaagat ggccgtatta
    16501 cgaaattaaa attaatcctg tctaatatag aacagattat tgaggaagac taggctccat
    16561 gcctagtctt tttgatgaaa agtgttataa tgaaaatgta aaaacgttta caagtaatga
    16621 aggagatgca atgactgaga aagattatgg acaagtaaca gggatggtgc attcgacaga
    16681 aagttttggt tctgtagatg gtccaggcat tcgttttatc attttcttgc aaggctgtaa
    16741 attacgttgc caatattgcc ataatccgga tacttgggag atggaaacca acaattcaaa
    16801 aatcagaaca gtcaatgatg tcttgaaaga agcccttcag tacaaacatt tctggggaaa
    16861 aaaaggagga attaccgtat ctggtggtga agctatgctt caaattaatt ttatcactgc
    16921 cttattcatc gaagctaaaa aattaggtat ccataccaca ctagacacct gtggctttgc
    16981 ctatagaccg acacctgaat accatcaggt tcttgataac ttattggcag tgaccgattt
    17041 gattttattg gacttaaaag aaattgatga aaaacaacat aaaattgtca cgcgtcagcc
    17101 taataaaaac atattgcaat ttgctaggta tttatccgac aagcagattc ctgtctggat
    17161 tcgacatgtc ttggttccag gcttgactga tattgatgat catttgacac gattgggtga
    17221 gtttgtcaaa accttgaaaa atgtcgataa atttgaagtt ttgccttacc ataccatggg
    17281 agaattcaag tggcgtgagc taggtattcc ttatcagtta gaaggtgtta aaccaccaac
    17341 taaggaacgc gttcaaaatg ctaaaaatct gatgcagacc gaatcttaca cagaatatat
    17401 gaatcggatt catcagtcat aagagctgtt atagggttga ggtttcctca actttttaaa
    17461 gtattataag cagatctttt gtaatcttcc tttgatttta gggggtaatt ttgctaaaat
    17521 aaaatgaata aacaaaatga ggtagaatga ttatgtcaaa aattttagtt tttggtcatc
    17581 aaaatcctga tacggatgct attgcatcat cttatgcttt tgattactta tcccaaaaag
    17641 cgtttggttt ggatactgaa gtcgtagctt taggaacacc taacgaagaa acagcttttg
    17701 cccttgatta ttttggtgtt gaggcaccac gtgtggtaga gtctgctaaa gcacaagggt
    17761 ctgaacaagt gattttgaca gaccacaatg aattccaaca atccattgca gatatccgtg
    17821 aggttgaggt atatggtgtg gttgatcacc accgtgtggc taattttgaa actgcaaatc
    17881 ctttatacat gcgtgtggaa ccagtaggat cagcctcatc tattgtttac cgtatgttta
    17941 aagaaaatgg cattgaggtg ccaaaagcga ttgcagggat gctcttgtct ggtttgattt
    18001 cagatacctt gttgttgaaa tctccaacaa ctcatgtgtc tgatcacctt gtcgctgaag
    18061 aattggcaga gctagcagaa gtgaacttgg aagactatgg aatggctctt ttaaaagcag
    18121 gtacaaacct tgcaagcaaa tcagaagtag aattgattgg tattgatgcc aaaacctttg
    18181 aattaaatgg caatgctgtg cgcgtggcac aagtgaacac tgttgacatt gcagaagtct
    18241 tagaacgtca agaagctatt gaagcagcca ttaaagatgc catggctgca gaaggttatt
    18301 cagattttgt tttgatgatc acggatattg ttaactcaaa ctctgaaatt ctagctattg
    18361 gagcaaacat ggacaaggtt gaagcagcct ttaacttcac ccttgacaac aatcatgcct
    18421 tcttagcagg tgccgtttct cgtaaaaaac aagtggttcc acaattgaca gaaagttttg
    18481 gagcttaatg aaaagacgct ttaaaaggac ttggtttttt tgaccaagtc cttttttcac
    18541 tcttctatgc tataatagaa ggtatgataa caatatttca ttcagataaa ctcaccagac
    18601 aacctttctt tcaagacctt atcaattatc tcgaccagca tgattacgtt atattacgag
    18661 agatcaaaaa agcctttcct aatgtgacag gtattgacaa ggccatcgaa agctatgttc
    18721 aagctggcta tattcgccgt gaaaataagc gttatggcat caatcttcct ttggtgagtt
    18781 ctgatcagca gctggcctta gacactatgc tttttgtgga cacctgttca gctatgtatg
    18841 aaaatatttt agcggttgtt tttgagactc agctaacaaa ccaaaccaat cacgtggtga
    18901 tcaaggaaaa gaccaacatc acgagagacg atttgaccct ggctaattat ttttaccgtc
    18961 tcaaaagagg tgagaagcca tcagctgagc agatggactt gtatgacctc ttgggggatg
    19021 tgaatcagga atatgccctt aaatatatga caactttttt gcttaaattc acgcgcaaag
    19081 accttgtgat gcaaaaacgt cctgatatat ttgtggaagc tctggttaca cttggttacc
    19141 taaaacaagt ggagcctaca acataccagc tgctaatgac cttggataaa gaaagtttaa
    19201 cctttatagc accatagaaa aaagctagga acaaggactg ttcttagctt tttgagtgct
    19261 tttattttgt ttttgccatt aagtaaagaa aataaggggc tccgataagc gacactaaaa
    19321 tccctgtctg gatattactg ccaaccagat agactcgtcc tactgtatct gcaacaagaa
    19381 gtaggatcat tccgattaaa atgctaatgg gtattgttat tttatgatta ctccctacca
    19441 agtatgtact aaagtgtcca gctattaagc cgataaaact aatattacca accaaaagaa
    19501 cactcaaagc acctaagcca gctgctaaga caagtatcaa acgacgctta cggtttaaag
    19561 ggagtcctag cccaatagca gtattatcag ctaatcccat aatattaagg aaatgggctt
    19621 gactataagt caatagccaa aaacacaata ataaaggagc gatgacactc agagtaggcc
    19681 agtcgtctcc tattaattgg ccgctaagcc aattgatgac caaatcgact ttgtagcgat
    19741 taatatgacc aacaagagca accattagac ttgaaagcat ggtagtaaca gctacaccag
    19801 taagaattaa ccgtgtagga tcaatttgtc cttgttttgt caaagatagc caataaacgc
    19861 taaaggttgt tacaacagca ccaagcatgg caaataaagg caagtaatgg ctgatatgag
    19921 cttgctcaaa gaaagaaaag gagaccacaa tagccaaaga gatacctgct cctatagtaa
    19981 tacctaatac accagagtca gctaagggat ttcgtgttaa acgttgtaat aatagccctg
    20041 ataaagcaag agaaccgcct cctagaaaag cagctagaat tcttggcaga cgaatgttaa
    20101 taacgatgaa agatatggca tggctagatt ttcccaaaaa cacatggaca aggtccaaaa
    20161 aagatagatg agattctcca agggacaaag ctaatcccat aagacttaca agtagcagga
    20221 tcaggctagt atataacctt aaaaggtgtc ggtgagattt caatagcggc ctcctcttct
    20281 tattaaccat aaaaagcatg gaaaaccaat taagctaacc aatgccccaa gaggggtttc
    20341 ataaggcgga ttgagattac gacaagcaat atcaacccat accatgaaac tagcgccaga
    20401 aactgcacaa agcggcaaaa ggtatcggta atgatgaggt gtaaagtgtt tcataagatg
    20461 aggaatgact aagccgataa aagaaatgct tccagcaatg gctacagctg ctgatgataa
    20521 aataagaacc aaaatcataa agactgcact gatcaagttg gtcttttgcc caagagcttt
    20581 tgcctgagac tcacttagac taaggacggt caggtgataa gataatagct gggctaaaca
    20641 aagactaaga ataataagag gagcaatata gccgatcatt tgccaattga ccccgacaag
    20701 acctcctgct tgccagccga taacagcatt tgtcagatga tagtaattgg taataccttg
    20761 acctaatgct gatagcagta tggaaaccat agctcctgct aagacaaggc ggagctgatg
    20821 gtagcctttg ccagattgat aagaaaggcc aaagactaag gttgctgcca ggctagaccc
    20881 taacaacgaa agcagaataa tgagggaata gtgcaagtga ggtacaaacg cataagccag
    20941 taccaaggct agtcctgctc cagcattaat gcctaaaagg ccaggctcag cgataggatt
    21001 acgtgtgatt gcttgcatga tagttccaga aacagctaac gcagagccag ttaaggtagc
    21061 accaaaaagt ctcggtaatc gtatagctag taaaacattg gctttatggc cgttttgctt
    21121 tccaaataaa atagagttaa gatcctgatg tgataaagca acggctccaa agcgcagatg
    21181 acagtaaata gcaacacata agctaaaact aagtagcaca aagacaagcc aaaagatatg
    21241 tgacttagtg attatctgcg ttttttttaa gcaaaaacgc tcgagacaca aaaagggatg
    21301 cttagttttc acttgataag attgcctctc gtaaagtctc taattgatat tctaaagaaa
    21361 gaggatcagt aaagtaaaag acattggcat tgactttgat cacatggtgt tttttcacag
    21421 ctggaatgga ttgccatagc ttgctttcat atagagcaga accagtctta tcatcttctg
    21481 cagcaacgac gacataatca acaatatagt ctggcaagac ttcctgcgac aatgacaggt
    21541 aaccttgttt aaaaacttca gtcttcactt tttcaggagc atcataatgg aaagcttgat
    21601 ggataacctc tccgccacgt ccccagtctt taccaaaaag gtaaacgtct ttttcataaa
    21661 gccccataat agtaaaggtt gctttgtcac ctgtaacggc cttgacttct ttttcataag
    21721 ctgctgtttt agttttccaa tcttttaacc atttcttggc tttgccttct ttgttaaaga
    21781 tgcggccgaa gtcagataac acttgtaaat aatcacgttt gcggtattca atcgaaataa
    21841 caggcgcaat ttctgccaat tgtttaatat tttcttctgt agagccaaca acaatgaggt
    21901 caggcttaag cgttgtaatg gcttctaaat cagttgcagc aacttgcttt gcttttttta
    21961 cagtctttgc taagatgggg tttttcttgt cataagaagt aaccccgact agattcatat
    22021 ctaatttttt gaggtaacca gtgtaagttg aagccagact gactactcgc ttaggctgtt
    22081 taggaatatc tccgtaataa gtcacaccag caatttgagg cataggcgat aaactttttg
    22141 aggcggtgtt agaatgatta gttgcttgat taccacaggc gattaaggta atggttgtca
    22201 aacagagtgt taatagtaga gttaattttt tcatgtatgc taattctcct agaataaagt
    22261 tattgaagct gataagtcag cactatagga cagttgtgga taggatcatc aaccagtacc
    22321 ggtttgattt ggaagatgtc ttgaatgata ggtgaagtca taacatctgc tatagtacca
    22381 gtataatgga tcttgccatg tttcatagca atcagatgat ccgaatagcg ggctgaaaga
    22441 ttgaggtcgt gaagaaccat aacaatagtc ttgccggcat ccttatttag ggatttaacg
    22501 agctctagga tttctagttg atgatttaaa tctaaataag tagttggctc atctaaaaaa
    22561 atagtatcag tgccttgggc taatgccatt gcaagccaaa ccctttgacg ttgcccccca
    22621 gaaagagcat caacaggttg atcagcatag gccatgacat tggtggcttg catagcccaa
    22681 tgaatggctt ttttgtcagc tggactaaga tttccaaagt aagattgatg gggaaaacgt
    22741 ccgtaagaaa ccaattcgta aacagttatg ccgttagttg cttcttgaac ttgaggtaaa
    22801 agagctagtt ttttagcaac ctctttagtt tctaatgtgg caatgttttg cccatttaaa
    22861 tacacaactc cctgttttgg tggtaataat cttgtcagtg cttttaataa actagacttc
    22921 ccacagccat tggcgccaat aatggtcgta attttacctt caggaatata aaacgataat
    22981 ttatcgatga tggtacgctg ttcataggca attgtgaggt cttcagcact aattgttgtc
    23041 atatggttgc ctcctttcta aaaagagtca aagggttgat gaaagtgcac attaaagtat
    23101 tcatctgccc attttaaaat agttgagaag tagcttattt tctcaaaatc ttcactatta
    23161 tagcatagaa aaaaggctta acaaagcctt ttttgatgtg atttttagga tgtattaaaa
    23221 aagaaatatt agtttagaat gattaaaaac aatagcgaga atcattctaa agtaacttca
    23281 acatactcta gaggtagtgt ttggcgattg ctaaatcacc agcataagct acttgttgtc
    23341 ctttaacaat ttgataagca tctgcgcctt tcccagcgat aatcacagca tcttttgctc
    23401 cttggcaaag agacatggct ttttgaatag cttgctcacg gtcactaata atctctaccg
    23461 ggcgtgcgat atggctggca atttcttttg aaatatcttc tggatcctcg aaattagggt
    23521 catcagcagt gagaatgacg gtgaggtttg gatgctgatg aatcacccta ccaaaatcag
    23581 ctcgtctgct ctcgccttta tttcctggtg cacctaaaat caacattaat ttgcctgtct
    23641 gatgttcttc cacaacgctg agtaattttt ctaagctgtc accattatgg gcataatcaa
    23701 caaaaacttt agcatgatta gtcatggtga ggacttccat acggcctggg acgcgagtct
    23761 tagcgatgcc tttttgaata tcagctaggc tagcacccaa acgaaggcag gcaagtccag
    23821 ctgccatagc attttcttgg ttaaaatggc caattaattg aatgtcataa tggccagcta
    23881 attgtccttt agcttcaaag gagaaagctt ggctagtggt gatctggttg tcagacaagg
    23941 gaccataaaa cacatgctct tgatccgcta cctgatcagc tagaaatgaa aaatgatcca
    24001 tcccgctatt gatcacaaca gctctgctat tctccattaa aagacgctta tgatagaaat
    24061 aatcttcaaa cgttggatgc tcgattgggc caatatgatc aggactaata tttaggaaaa
    24121 ccccaacatc gaacgtaagc ccatagacac ggtcaacgag gtaagcctga ctagaaactt
    24181 ccataatcaa gtgggtcatg ccattagtca cacactcagc catcatagca aaaagatcca
    24241 agctttcagg agttgtaagt tgtgatttga aaaaggtctt gccatctaag gtggtattca
    24301 tggtagaaaa catggcaggt ttataggatt ctttcaacat gtggtaggca aaataagcag
    24361 ccgtcgtctt tcccttagta cctgtaaaag ctagtagttt gagcttttct tgagggttgc
    24421 catagaaagc catggcaatt agactcatag cttttttgat atcagtgacc aaaacaacgg
    24481 gaataccaag ctcgtaatct acctctgaaa tatagagttg taatccgtta gtgattgctt
    24541 ctttaaggta gtcagctttg aaagttgctc ctttagcaaa aaaaagggtc ttgccatcta
    24601 cttgacggct atcgtaactc aagcgctcaa aacttaagcc ttgataatgg tagtggtatc
    24661 cgtcggcatc aagaacctca cgaaaattat ggtctttttt taaaatgtct aataattgtt
    24721 caatggttat cataccctta ttttatcctt aaagttcttg ttttacaagt cacaagcaaa
    24781 agtttataat aaaactaata gaaactagga aagaatagat atgtcgacag aaaaaaaaca
    24841 actgacacaa gaagaattga tggttcaggg agcagcctgg tccacagctg gtaatttcat
    24901 tagccgactt ttgggagtgt tatacattat tccctggtat atttggatgg ggcaatacgc
    24961 gattcaagcc aacgctcttt tcaatatggg gtataatgtt tatgcttatt ttttattgat
    25021 ttcaaccaca ggactcaatg ttgcgattgc taagcaggtt gccaagtaca attccatggg
    25081 acaaactgag catagctatc aattaatccg aagtacccta aaactcatgc ttggtctggg
    25141 ccttattttt tcagtcatta tgtatctggg ctctccttta tttgcgagct tatctggtgg
    25201 tgatgacaca cttgttccca ttatgcacag cctttcttta gcagttttta tttttcctgt
    25261 aatgagtgtg attcgaggta tctttcaagg ccacaataat atcaagcctt atgcgattag
    25321 tcagattgct gagcaactca ttcgggttat ctggatgctt ttgaccacct tttttatcat
    25381 gaagttaggt tcaaaagatt atctatcggc ggtaacgcag tcaaccttgg cagcctttgt
    25441 gggaatgatt gctagtatgg ctgtcttagg ttactacttg tggaagttag gcttgctaaa
    25501 agcaatatgt cgtaagccca atcatcatat tgatagtgac attaaaggac tgttgcttga
    25561 aaccttaaaa gaatccatcc cctttatcgt gacaggaagc gcgattcaag cctttcaatt
    25621 gattgatcaa tggacttttg tcaatactat gactcttttt acagattata gccgttctca
    25681 attgcttgtt ttatttggtt actttaacgc aaacccagcc aaaattacaa tggttttaat
    25741 tgcagtagca gcatctattg gtggtgtagg cattgcctta ttaactgaaa attatgtcaa
    25801 aaaagatatg aaagcagctg ctcgcttaat cattaacaac attgaaatgt tagtgatgtt
    25861 tttgttaccc gctcttactg gggcaattat tttagcaaga cctctatatt ctgtttttta
    25921 cggagctagc gaggatcgtg ctattcacct ctttgtggcg gtcctcttcc aaaccttgct
    25981 actggcgctt tacaccctct tttcaccgat gcttcaagct ctttttgaaa atcgaaaagc
    26041 gatttactac tttgcctatg gtatcttgat taagttagtt ttacagatac cgcttattta
    26101 tttgctacat gcttatggtc ctttactagc gacgacgatt gctttagtgg tgccgattta
    26161 tttgatgtat cgacgcctat atcaggttac tcattttaac cgcaaactgt tgcaaaaacg
    26221 tttattatta accttgattg aaaccttatt aatgggactg gtcgtgtttg tggccaactg
    26281 gctattgggc tatgccttta aaccgacagg ccgcttgacc agccttcttt acctcctcat
    26341 tattggtggc ttgggaatga cggtttacac ggcactcacc ttgctgacgc atcaactaga
    26401 taaattaatt ggtagcaaag ctagtcgcct tcgtcagaaa ttaggctggc attaatataa
    26461 tgacaccaag aacggtttcg tgagtgttta tcgaaatcgt tttttcttaa tgatctcgct
    26521 tagtaaaggt agctgataaa aaatcaggat ttcccaagca aactactgat atttttggta
    26581 taatgataaa agtgtaaaaa aatagaagga agtatatcaa catgggaaaa tgtcaagtta
    26641 tttcgcatcc gcttatccaa cacaaacttt caatcttacg tcgtcaaacc acatcaacta
    26701 aagattttcg tgagttggtt aatgaaattg cgatgctgat gggatatgaa gtgtcacgcg
    26761 accttccctt agaagatgtc gacatccaaa cgccagtctc aaaaacggtc caaaaacagt
    26821 tagctggtaa aaaattagct attgttccga ttcttcgtgc tggtattggg atggttgatg
    26881 gcttgttaag tttggtacca gctgcaaaag ttggccatat cggcatgtac cgtaatgaag
    26941 aaacccttga acctgttgaa tacctcgtta aactaccaga agatattaac caacgacaaa
    27001 ttttccttgt tgatccaatg ttggcaacag gtggttcggc tatcttagca gtggattcgt
    27061 tgaaaaaacg tggcgcagca aatattaagt ttgtctgttt agttgcagca ccagaagggg
    27121 tgaaaaaact tcaagaagct catccagata ttgatatctt cacagcagca cttgatgatc
    27181 atttaaatga acatggctat atcgtgccag gcttgggcga tgctggtgat cgtttatttg
    27241 ggacaaaata aaagagacaa ccatcaaata gtagcctttt taggccaatc gttcttgatt
    27301 ttgttaaaaa aatcagagag atttctcttc tttaaaggaa aagcaagttt atgatttgac
    27361 ctttattgac caaaatatta taataaggac aatcagtaaa aaagagatga cctagtgggt
    27421 tgccgtctct cacaatgacg ctactaaaaa cccttgtaga ttggagaatg tattatgatt
    27481 cctgttgtta ttgaacaaac tagtcgtgga gaacgttctt atgacattta ttcacgttta
    27541 ttaaaagacc gtattatcat gttaacaggg cctgttgaag acaacatggc caactctgtc
    27601 atagcgcagc tcttattcct tgacgcgcaa gacaacacga aagatatcta cctatatgtc
    27661 aacacaccag gtggctctgt ctcagcaggt ttggctattg tcgataccat gaactttatc
    27721 aaggccgatg tgcaaactat tgtcatgggt atggcagctt ctatggggac agtcattgct
    27781 tcatcaggaa caaaaggcaa acgctttatg ttaccaaatg cagagtatat gatccaccag
    27841 ccaatgggtg gcacaggcgg tggcacgcaa caaacggata tggctatcgc agcagaacat
    27901 ctcttaaaaa cacgtcaccg tttagaaaaa atcttagcgc aaaatgctgg aaaaacgatt
    27961 aaacaaatcc ataaagatgc tgagcgtgat tattggatga gtgctgaaga aaccttggct
    28021 tacggtttca ttgatgaaat catggaaaac aacgaactaa aataaagagg atctctgcaa
    28081 aagtattcta gaaacttcta ggatgctttt tctcttagaa aggaaaataa tgatacagcc
    28141 tactgcatta aaacagcttc aagagttaaa acgtcagttc agatcccttt gcaaccaatt
    28201 atgagatagt ttgaggatga tccttagcag ctgtgtgcaa gaagttatga cgatttcata
    28261 aaggatgacc tagccagtct ttcttcgtct cttttactta aagtgtggta taatcagatt
    28321 atgctagcca aaactaaaaa atgcattgcc attaaaggtg ttgtagatcg gcttatccta
    28381 gatctcataa caatgctgta gtaccgccct taccttgcta ctaaaggcgc tgttgaagca
    28441 caaattgact tgcaaaggat cttgactgat aggtatatgt ccggtggaat cgccaaagca
    28501 gcaaaaagac caagaccctt atttggtctc taccaaaata taatagaaaa ggatttagaa
    28561 tgtttcaaaa acaagaacgt attggactcg tcgtttacct gtattataac cgagatgcca
    28621 gaaagttatc aaaatttggt gatttatatt accattccaa gcgctctcgt tacctcatta
    28681 tttatattaa taaaaatgat ttagacacaa aattagaaga aatgagacgt ttgaaatgtg
    28741 ttaaagacat tagaccatca gcttttgatg atattgaccg ccaatttgta ggtaaccttc
    28801 atcgcgacga aacaaacaac catcaaaagg gttaccaacc ccctagttac tgattagcgt
    28861 ttcagattta gttagcttat taaagtaaga aggttttgac agatgctcac actacttcaa
    28921 gacaatgaga caactagagc attagtgaat aactgagttg actgtcactt ctttaataag
    28981 aacggatagg attggtccta aaatagagca ctgcaaagat atgccagtgc tttttagctc
    29041 attttttgat aaaatagaac tactaaaaag tgaaggaaat aagatgatca caggaaaact
    29101 cataaccgtt gaaggtccag atggcgcagg gaaaacgact gttttagagc aactcattcc
    29161 actgttaaaa caaaaagtga ctcaggagat cttgacaaca agagagcctg gaggtgttgc
    29221 catttcagaa tacattagag aacttatttt agacatcaac catactacta tggaccctaa
    29281 aacagaactc ttactttata ttgcggctcg aaggcagcat ttggtggaaa aggtactacc
    29341 agctcttgaa gcaggccagt tggtttttat tgatcgtttc atcgatagtt cagtagctta
    29401 tcaaggtgcg ggacgcggtt tgatcaaagc agacattcag tggctcaacg agttcgcaac
    29461 agatggttta gagcctgact tgacccttta ttttgatgtt ccttctgaaa ttggtttagc
    29521 acggattaac gcgaaccaac aaagagaagt caatcgttta gatttggaaa caatagagat
    29581 acatcaacga gtacgaaaag gatacctagc cttagctaag gagcatccaa aacgtattgt
    29641 cacaattgat gcgactaaac cattgaaaga agttgtctct gtcgctttgg aacatgtctt
    29701 agccttgctt ttagcctagg ataagggagg gtaggaatgg atttagcgca aaaagctcct
    29761 aacgtttatc aagcttttca gacgatttta aagaaagacc gtctgaatca tgcttatctt
    29821 ttttcgggtg attttgctaa tgaagaaatg gctctttttt tagctaaggt catcttttgt
    29881 gaacagaaaa aggatcagac gccctgcggg cattgtcgct cttgtcaatt gattgaacaa
    29941 ggagattttg ccgatgtgac ggtattggaa ccaacagggc aagtgattaa aacggatgtg
    30001 gtcaaagaaa tgatggctaa cttttctcag acaggatatg aaggaaaacg acaagttttt
    30061 attatcaaag attgtgaaaa aatgcatgta aatgccgcta atagcttgct aaaatacatt
    30121 gaggagcctc agggagaagc ttacatgttt ttgctgacca atgatgataa caaagtgctt
    30181 ccgaccatta aaagtcggac acaggttttt cagtttccta aaaacgaagc ctatctcttc
    30241 cagttagccc aagagcgagg tttattaaga agtcaagctg atttagtggc caaactggct
    30301 aaaaatgttg accaattaga agatttggca caaaatagtc attttttaga agtgatggct
    30361 cagactgaac gttttgtttc aatctggtta aaagatcagc tgcaagctta tttagccttg
    30421 aatcacctgg tactgttggc aaccgaaaaa gaggagcaag agttaatttt gacccttttg
    30481 acactactct tggcaagaga gcaatcacaa acgcccttta aacaattgga ggcggtttat
    30541 caggctaggc tcatgtggca gagcaatgtc aattttcaaa acacattaga atatatggtg
    30601 atgtcagaat aagtgcaatt ggaattcgtt atggggaaac gaagcttgtc aaaagacttg
    30661 cccgtagcga ccaaagtagc ggcgcatagg ggcaaaaaag cttttgaaga aaatactgcc
    30721 cttgcaacag atttgatgaa agaagtatca cgattaatga tacaaaatca acttgaaatg
    30781 acgtttatta atagtgtctt tcccttagat cagcatgttg tcctaattcc cttttcagca
    30841 gaagagcaca tggattttta caactgttaa aagatttagc ggccgatttt aggtcaagaa
    30901 gtgagctgcc ccagattaac agccaagaag aaaccaagat ttatgggcgc ttaggaccgt
    30961 gtggcagacc actgtgctgc tttcgttttt gggaaaattt ctgacagtat ctattaaaat
    31021 actaaaaaat caaaggttat cattgacttt gggtaaagca acaagccctt atggccgttt
    31081 gctttgctgt ctctatgatg aaggtggctt ttaccaataa agcaagaaaa aattccctga
    31141 atatggtacc atagtagaga caagtgatgg ccctgcaaca gtagcagccg ttgatatatt
    31201 tacagatccc attaaggtgc ggttaaaaga ccagttgacc ttagtcactt atgctttaga
    31261 ggaggttaaa gtaggtgaat aaaaaagaat tattcgatgc ttttgatgga ttttcccaaa
    31321 atttaatggt tactttagct gaaattgaag ccatgaaaaa acaagtccaa tcactagtag
    31381 aagaaaacac gattcttcgc ctagaaaata caaaactacg ggaacgtttg agtcacctag
    31441 aacatgaaac ggtagctaaa aatccttcca aacaaaggaa ggatcactta gagggaattt
    31501 atgatgaagg gtttcatatc tgcaattttt tctatggaca acgtcgtgaa aatgatgaag
    31561 aatgtatgtt ttgcagagaa ttattagata gaaagtaagg gcatgcaggt tcaaaaaagt
    31621 tttaaagaca aaaaaacaag cggaaccttg tatttggttc caaccccaat tggcaatctg
    31681 caggacatga cctttagagc agtggcaacg ttaaaagagg ttgattttat ttgtgcagaa
    31741 gacacaagaa acacaggtct tttgctaaaa cactttgaca ttgctaccaa gcaaatcagc
    31801 tttcatgagc acaacgccta tgagaagatt cctgatttaa ttgatttatt aatatcagga
    31861 cggtcactag ctcaggtatc tgatgcagga atgccatcga tttctgatcc tgggcatgat
    31921 ttggtcaagg ctgctattga tagtgatatc gcagtcgttg ctttaccagg tgcctcagca
    31981 gggattacag ctttgattgc tagtggactg gctcctcaac cccatgtttt ttatgggttt
    32041 ttacccagaa aagcagggca acaaaaggct ttctttgagg acaaacacca ttacacagaa
    32101 acacaaatgt tttatgaatc accctatcgc atcaaagata ccctgaccaa tatgttggct
    32161 tgttatggtg atcgtcaagt ggtgcttgtt agagaattaa ccaagctttt tgaggagtac
    32221 caacgggggt cgatttcaga aatcttgtcc taccttgagg aaacacccct aaaaggggag
    32281 tgtctgctga ttgtggctgg agcccaagta gacagtgaga ttgagctgac agctgatgtt
    32341 gacttagtgt ctctagtgca aaaagaaatc caagcaggag ccaagccaaa tcaggctatt
    32401 aaaacaattg ctaaagctta ccaggtaaat cgccaagagc tttaccaaca atttcatgac
    32461 ttataagtca tcgtagatta ggagaaaaac aaatgacaga agatcaagca actatcctta
    32521 ttttaggagt atacggaatc atattggctg tattcttatt atcagcttta ttatgcctta
    32581 tcgctaactg gcgactcttt aaaagagcag gttacaaagg atggtatagt ctcattcctt
    32641 tctatgcagg ttttgtcaaa cacaaaatca cttttggaga agataataaa tggttttact
    32701 ttattggttg ggtcattgct atttattatt actatgtgca cttttgctat gtccgtgctt
    32761 ttggtggatc aaagggtcta gcagtatttg gcttgttttt cccaggcatt gcttctttga
    32821 tagtggcttt tggaaaaact tataccacac aagaatgcca ccctgtgcca catcttttaa
    32881 actaagagaa aaaagaccat caattggatt tattgatggt cttttaggtg tttaaatgat
    32941 tttagtcccg tgaacaatgg ccgttccagt gagttgactc aagttttggc aattctcagc
    33001 agtgacgcct cctccaatca tgatttcaat gcgtttattg gcataggtca caagactttt
    33061 aagctgttcc acattatctg taataggagt agcttcaggg gagccatgag ttaacacacg
    33121 cacaaagcca taatcaatca gttgatcaag cgcttggtgc tggtggtcag tcggaatatg
    33181 gtcaaaagcc atgtggaaaa ccagtggcaa gccttgtgta gcaggcagta actgctcgat
    33241 agcgtcggta tcaaggtgat tttctgtggt cagcaagcct aagactagcg catctgagcc
    33301 agcttcaaca gcttttaaga tatcttcttc catagctttt agttcgaggt cgttatagac
    33361 aaaatcgcca ccgcgaggtc gaatcatggt cgctacgcta atctttttat cgtgtaagag
    33421 ctgacaggct tctttgatga cgccataaga tggtgttgtt cccccgacag ctaggttatc
    33481 acagagttcc acacgactga cttgaccagc atcaagggtt ggcagtagtg tgaggttctc
    33541 agcacaaaat tctttaatca tttttagatc tcctttatca ctaatattat agcacgaatg
    33601 aagctagtca tcaggaaaca tttttgtcaa atcatggaca ttatgttata ctcactagta
    33661 ttaaaagtag gccaaaacag cagcaaatac tttgaaaaaa gattgctatg ttctacggca
    33721 atatcagtct ttaccaacga actctcaaga agaaaagaaa gacaaagctt agcattttaa
    33781 aaaagccccc ctttttttgt gaaatgatga ccaaaaacag gcgcctcacg cctaaaacag
    33841 ttaccctaaa ggagaacaaa tgattacgat ttaccaatac ccaaaatgta gtacctgtca
    33901 aagagccatg gctgaactaa agcaactggt tagtgacttt gaagtcattg acatcaaagc
    33961 taatccaccc aaagctcagg acctcaaaca ttggatggaa acatctggtt ataccatcaa
    34021 aaatttcttt aataccagtg gtaacagcta ccgcgaattg ggacttaaag ataaaataga
    34081 ccagcttagc cttgataaag ctgctgaact cttagccaca gatggtatgc tgattaaacg
    34141 gcctattctg attaaggacg gtagagtcct acaagttggc tatcgcaagc catatcaaga
    34201 gttggattta ggataatgcg acaaagtctc aaaaaaggac aaggcaacat tataacgcct
    34261 tgtccttttt tgggatctgt ttttagaaat tagagatcaa tatctagtaa aattggcgta
    34321 tggtcttggc gttccccaga agaaatcatt tcagaacgct taaccttatc cacaagtcgg
    34381 tttgaagcta gccaatagtc aattctccaa ccggtattat tgattttgct ggttttgcta
    34441 cgttgtgccc accaggtata aacatgagga atatcgccgt gcaagtgacg aaaagtatct
    34501 gtaaagcctc ttgctaatag gttagtaaag ccttgacgtt cttcgtcggt aaaaccaggg
    34561 gaacgtcgat tgctgcttgg atttgccaaa tcaatctctt tgtgggcaac gttgtaatct
    34621 cccgtagcca aaacaggctt ttgagcatcc agttcagtca agtaatcagc gtatttgtgg
    34681 tcccaaattt gacggtcatc taaacgtcta aggccatcac cagcattagg cgtgtaaacc
    34741 tgtgtgacga aaaagtcttc aaattctagg gtaatgatac ggccctcagc atccattgtt
    34801 gttggggcac caatttctgg aaaagtaatg acagggttta gcgtgttttt gtacaggaac
    34861 atggtgccag catagccttt cctagcaggc tcaactgaag aacgccacac atgcaaatag
    34921 tttggaaagt aactaagtaa ggcctcgata tgttttttgg taggcccctt agcagataat
    34981 ttggtttctt gaatggcaat aatatccgca tcttgagcga ctaaagtgtc taaaacagcg
    35041 cgtgataaaa gcgcacgagg ggactctcct gtgagagcag catttaagga atcaatgttc
    35101 catgaaatca gtttcatttg gtatctcttt ctaacagata gtcttgttgt tattgtaaca
    35161 aaaaacgaaa cgataagcga ctttggacac ctagccccca taaaacagtc tagccaaatg
    35221 aagcatagca gcgtaagaac aagataagga ggtgatcagt gagcatatcg tttgacagtt
    35281 aagagtagga ttactataat agaagcataa atgtaagcgc taacaaaata aaaaatatag
    35341 gagaatgtca atggcacaaa aaacagtcat caccgaggaa acaaccgatt ttgttatgga
    35401 tttcaaaaca agtagcgctg agggaaatgt tgattttatt aacgtttttg accttgagaa
    35461 aatggcacaa caggtcatcc ctaaaggagc ctttggttat attgctagtg gtgctggtga
    35521 tacctttacc ctccatgaaa atattcgctc atttaatcat aaattaattg tccctcactc
    35581 cctcaaaggg gttgaaaatc cgtcaacaga gattacgttt gatggtgatc acttaacctc
    35641 tcctcttatt ttagcacctg tagcggctca taaactggcc aatgaacaag gagaagtagc
    35701 gtctgccaaa ggattgaagg aatttggaag tatctacacc actagttcct actcaacaac
    35761 tgacctgcca gaaatcagtg ctgccttagg tgggacaccc cattggtttc aattttatta
    35821 cagtaaagac gatggcatta atcgtaatat tatggatcgt gtcaaagcac aaggttgcaa
    35881 agctatcgtc ttgacagcag atgcaacagt tggaggaaac cgcgaagttg ataggcgaaa
    35941 cggttttgtt tttccggtcg gtatgccaat tgttcaagaa tatttacctg atggtgcagg
    36001 taaaacaatg gattatgtct ataaatcagc taaacaagcg cttacctcaa aagatattga
    36061 atatatcgca acctactcag gactgcctgt ttatgtcaaa ggaccacagt gtgctgagga
    36121 taccttgcgg gcattggacg caggagcatc aggtatctgg gtaaccaatc atggtggccg
    36181 tcaattagat ggtggtccag ctgcttttga ttccttacaa gaagtcgctg aagcagttga
    36241 tcaaaaagtt cctattgtct ttgactcagg tataagacgg ggtcaacata ttttcaaagc
    36301 tcttgcttca ggtgctgacc ttgttgcctt ggggcgtcca gcaatttacg gcttggctat
    36361 gggaggaagc actggtactc gtcaggtctt tgaaaagtta aatgacgagt taaaaatggt
    36421 tatgcaactg gctgggacac aaactatcca agatgttaaa gcctttaacc ttcgccataa
    36481 tccgtatgat tcatcgattc catttgatca aaatgcgcta cgcttagatt aaaacagaca
    36541 aaaacctctg ctatcatgtc acaaatagta gaggttttac actgcaaaaa gaggtatgaa
    36601 aatccaataa catagttaca aggctatcgt ttaccacccc actaatagat taaaatgaaa
    36661 gacttcttac attaatagaa gtttctgttg cttttctcag aaaaatgtgg ttaaataatc
    36721 aaatattatt aacgaaaaat gagttaatga ttaacattaa actggtttta gttaaaacat
    36781 ttaggagggt atcaggtgga gaaaaagcaa cgtttttccc ttagaaaata caaatcagga
    36841 acgttttcgg tcttaatagg aagcgttttc ttgatgatga caacaacagt agcagcagat
    36901 gagctaacca caacgagtga accaacaatc acgaatcacg ctcaacaaca agcgcaacat
    36961 ctcaccaata cagagttgag ctcagctgaa tcaaaacctc aagacacatc acaaatcact
    37021 cccaagacaa atcgtgaaaa agagcaatca caagatctag tctctgagcc aaccacaact
    37081 gagctagctg acacagattc agcaccaatg gctaatacag gttctgatgc gactcaaaaa
    37141 agcgcttctt taccgccagt caatacagat gttcacgatt gggtaaaaac caaaggagct
    37201 tgggacaagg gatacaaagg acaaggcaag gttgtcgcag ttattgacac agggatcgat
    37261 ccggcccatc aaagcatgcg catcagtgat gtatcaactg ctaaagtaaa atcaaaagaa
    37321 gacatgctag cacgccaaaa agccgccggt attaattatg ggagttggat aaatgataaa
    37381 gttgtttttg cacataatta tgtggaaaat agcgataata tcaaagaaaa tcaattcgag
    37441 gattttgatg aggactggga aaactttgag tttgatgcag aggcagagcc aaaagccatc
    37501 aaaaaacaca agatctatcg tccccaatca acccaggcac cgaaagaaac tgttatcaaa
    37561 acagaagaaa cagatggttc acatgatatt gactggacac aaacagacga tgacaccaaa
    37621 tacgagtcac acggtatgca tgtgacaggt attgtagccg gtaatagcaa agaagccgct
    37681 gctactggag aacgcttttt aggaattgca ccagaggccc aagtcatgtt catgcgtgtt
    37741 tttgccaacg acgtcatggg atcagctgaa tcactcttta tcaaagctat cgaagatgcc
    37801 gtggccttag gagcagatgt gatcaacctg agtcttggaa ccgctaatgg ggcacagctt
    37861 agtggcagca agcctctaat ggaagcaatt gaaaaagcta aaaaagccgg tgtatcagtt
    37921 gttgtagcag caggaaatga gcgcgtctat ggatctgacc atgatgatcc attggcaaca
    37981 aatccagact atggtttggt cggttctccc tcaacaggtc gaacaccaac atcagtggca
    38041 gctataaaca gtaagtgggt gatccaacgt ctaatgacgg tcaaagaatt ggaaaaccgt
    38101 gccgatttaa accatggtaa agccatctat tcagagtctg tcgactttaa aaacataaaa
    38161 gatagcctag gttatgataa atcgcatcaa tttgcttatg tcaaagagtc aactgatgcg
    38221 ggttataacg cacaagacgt taaaggtaaa attgctttaa ttgaacgtga tcccaataaa
    38281 acctatgacg aaatgattgc tttggctaag aaacatggag ccctgggagt acttattttt
    38341 aataacaagc ctggtcaatc aaaccgctca atgcgcctaa catctaatgg gatggggata
    38401 ccatctgctt tcatatcgca cgaatttggt aaggccatgt cccaattaaa taccaatggt
    38461 acaggaagtt tagtgtttga cagtgtggtc tcaaaagcac cgagtcaaaa aggcaatgaa
    38521 atgaatcatt tttcaaattg gggcctaacc tctgatggct atttaaaacc tgacattact
    38581 gcaccaggtg gcgatatcta ctctacctat aacgataacc actatggtag ccaaacagga
    38641 acaagtatgg cctctcctca gattgctggc gccagccttt tggtcaaaca atacctggaa
    38701 aagactcagc caaacttgcc aaaagaaaaa attgctgata tcgttaagaa cctattgatg
    38761 agcaatgctc aaattcatgt taatccagaa acaaaaacga ccacctcacc gcgtcagcaa
    38821 ggggcaggat tacttaatat tgacggagct gtcactagcg gcctttatgt gacaggaaaa
    38881 gacaactatg gcagtatatc attaggcaac atcacagata cgatgacgtt tgatgtgact
    38941 gttcacaacc taagcaataa agccaaaaca ttacgttatg acacagaatt gctaacagat
    39001 catgtagacc cacaaaaggg ccgcttcact ttgacttctc gctccttaaa aacgtaccaa
    39061 ggaggagaag ttacagtccc agccaatgga aaagtgactg taacggttac catggatgtc
    39121 tcacagttca caaaagagct aacaaaacag atgccaaatg gttactatct agaaggtttt
    39181 gtccgcttta gagatagtca agatgaccaa ctaaatagag taaacattcc ttttgttggt
    39241 tttaaaggac aatttgaaaa cttagcagtt gcagaagagt ccatttacag gttaaaatct
    39301 caaggcaaaa ccggttttta ctttgatgaa tcaggtccaa aagacgatat ctatgtcggt
    39361 aaacacttta caggactcgt cactcttggg tcagagacca atgtgtcaac caaaacgatt
    39421 tctgacaacg gtctacacac acttggtacc tttaaaaatg cagatggcaa atttatctta
    39481 gaaaaaaatg cccaaggaaa ccctgtctta gccatttctc caaatggtga taacaaccaa
    39541 gattttgcag ccttcaaagg tgttttcttg agaaaatatc aaggcttaaa agcaagtgtc
    39601 taccatgcta gtgacaagga acacaaaaat ccactatggg tcagcccaga aagctttaaa
    39661 ggagataaaa actttaatag tgacattaga tttgcaaaat cgacgaccct gttaggcaca
    39721 gcgttttctg gaaaatcgtt aacaggagct gaattaccag atgggtatta tcattatgtg
    39781 gtgtcttatt acccagatgt ggttggtgcc aaacgtcaag aaatgacatt tgacatgatt
    39841 ttagaccgac aaaaaccggt actaccacaa gcaacatttg atcctgaaac aaaccgattc
    39901 aaaccagaac ccctaaaaga ccggggatta gctggtgttc gcaaagacag tgtcttttat
    39961 ctagaaagaa aagacaacaa gccttataca gttacgataa acgatagcta caaatatgtc
    40021 tcagtagaag acaataaaac atttgtggag cgacaagctg atggcagctt tatcttgccg
    40081 cttgataaag caaaattagg ggatttctat tacatggtcg aggattttgc agggaacgtg
    40141 gccatcgcta agttaggaga tcacctacca caaacattag gtaaaacacc aattaaactt
    40201 aagcttacag acggtaatta tcagaccaaa gaaacgctta aagataatct tgaaatgaca
    40261 cagtctgaca caggtctagt cacaaatcaa gcccagctag cagtggtgca ccgcaatcag
    40321 ccgcaaagcc agctaacaaa gatgaatcag gatttcttta tctcaccaaa cgaagatggg
    40381 aataaagact tcgtggcctt taaaggcttg aaaaataacg tatataatga cttaacggtt
    40441 aacgtatacg ctaaagatga ccaccaaaaa caaaccccta tctggtctag tcaagcaggc
    40501 gctagtgcat cagctattga aagtacagcc tggtatggca taacagcccg aggaagcaag
    40561 gtgatgccag gtgattatca gtatgttgtg acttatcgtg acgaacatgg taaagaacat
    40621 caaaagcagt acaccatatc tgtgaatgac aaaaaaccaa tgatcactca gggacgtttt
    40681 gataccatta atggcgttga ccactttact cctgacaaga caaaagccct tggctcatca
    40741 ggcattgtcc gcgaagaagt cttttacttg gccaagaaaa atggccgtaa atttgatgtg
    40801 acagaaggta aagatggtat cacagttagt gacaataaga tgtatatccc taaaaatcca
    40861 gatggttctt acaccatttc aaaaagagat ggtgtcacac tgtcagatta ttactacctt
    40921 gtcgaagata gagctggtaa tgtgtctttt gctaccttgc gtgacctaaa agcggtcgga
    40981 aaagacaaag cagtagttaa ctttggatta gacttaccgg tccctgaaga caaacaaata
    41041 gtgaacttta cttaccttgt gcgggatgca gatggtaaac cgattgaaaa cctagagtat
    41101 tataataact caggtaacag tcttatcttg ccatacggca aatacacggt cgaattgttg
    41161 acctatgaca ccaatgcagc caaactagag tcagataaaa tcgtttcctt taccttatca
    41221 gctgataata acttccaaca agttaccttt aagatgacga tgttagcaac ctctcaaata
    41281 actgcccact ttgatcatct tttgccagaa ggcagtcgcg ttagccttaa aacagctcaa
    41341 ggtcagctga tcccgcttga acagtccttg tatgtgccta aagcttatgg caaaaccgtt
    41401 caagaaggca cttacgaagt tgttgtcagc ctgcctaaag gctaccgtat cgaaggcaac
    41461 acaaaggtga ataccctacc aaatgaagtg cacgaactat cattacgcct tgtcaaagta
    41521 ggagatgcct cagattcaac tggcgatcat aaggttatgt caaaaaataa ttcacaggct
    41581 ttgacagcct ttgccacacc aaccaagaca acgacctcag caacagcaaa agccctacca
    41641 tcagcgggtg aaaaaatggg tctcaagttg cgcatagtag gtcttgtgtt actcggactt
    41701 acttgcgtct ttagccgaaa aaaatcaacc aaagattgat aacaaaacgc aaagagacaa
    41761 caaatcatcc cttttaacag tcggaccttt ctttgaaatt gagctggcag atgatcattt
    41821 cttaacaaaa gttaaagagt tgagcaaagt aaggaacaga cataaagacc ataaa
//
LOCUS       DAUCLR010000004        40800 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.004, whole genome shotgun sequence.
ACCESSION   DAUCLR010000004 DAUCLR010000000
VERSION     DAUCLR010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40800
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.004"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            152..1966
                     /gene="glmS"
                     /locus_tag="VGT87_000148"
     CDS             152..1966
                     /gene="glmS"
                     /locus_tag="VGT87_000148"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019804802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEQ3813040.1"
                     /translation="MCGIVGVVGNRNATDILMQGLEKLEYRGYDSAGIFVANANQTNL
                     IKSVGRIADLRAKIGIDVAGSTGIGHTRWATHGQSTEDNAHPHTSQTGRFVLVHNGVI
                     ENYLHIKTEFLAGHDFKGQTDTEIAVHLIGKFVEEDKLSVLEAFKKALSIIEGSYAFA
                     LMDSQATDTIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETSEFMEIHDKELVILTKD
                     KVTVTDYDGKELIRDSYTAELDLSDIGKGTYPFYMLKEIDEQPTVMRQLISTYADETG
                     NVQVDPAIITSIQEADRLYILAAGTSYHAGFATKNMLEQLTDTPVELGVASEWGYHMP
                     LLSKKPMFILLSQSGETADSRQVLVKANAMGIPSLTVTNVPGSTLSRESTYTMLIHAG
                     PEIAVASTKAYTAQIAALAFLAKAVGEANGKQEALDFNLVHELSLVAQSIEATLSEKD
                     LVAEKVQALLTTTRNAFYIGRGNDYYVAMEAALKLKEISYIQCEGFAAGELKHGTISL
                     IEEDTPVIALISSSQLVASHTRGNIQEVAARGAHVLTVVEEGLDREGDDIIVNKVHPF
                     LAPIAMVIPTQLIAYYASLQRGLDVDKPRNLAKAVTVE"
     gene            2162..2497
                     /locus_tag="VGT87_000149"
     CDS             2162..2497
                     /locus_tag="VGT87_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEQ3813041.1"
                     /translation="MSLPNCLQCQSEYVYEDGLLLICPMCAFEWTPGEEATEEEGLVV
                     LDSNGVRLSDGDTITVVKDLKVKGAPKDLKQGTRVKNIRLVEGDHNIDCKIDGFGAMK
                     LKSEFVKKI"
     gene            2604..3245
                     /locus_tag="VGT87_000150"
     CDS             2604..3245
                     /locus_tag="VGT87_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEQ3813042.1"
                     /translation="MTYIQQVLPSLLDGALVTLQVFFIVIILSIPLGAILAFLMKIPF
                     KPLQWFLTLYVWMMRGTPLLLQLIFFYYVLPSVGISFDRMPAAILAFTLNYAAYFAEI
                     FRGGIEAIPKGQYEAAKVLKLKPLQTIRYIILPQVFKIVLPSVFNEVINLVKDSSLVY
                     VLGVGDLLLASKTAANRDATLAPMFIAGLIYLLLIGLVTIISKQVEKRFNYYQ"
     gene            3255..3884
                     /locus_tag="VGT87_000151"
     CDS             3255..3884
                     /locus_tag="VGT87_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEQ3813043.1"
                     /translation="MLELKNISKQFGQKTIFDGFNLTVQDGEVLSLVGPSGGGKTTLL
                     RMLAGLESIDSGQVFYNGEDVGIDHLENRNLLGFVFQDFQLFPHLTVLDNLTLSPTIT
                     MGKQKADAKEKALDLLARLGLKEHAQVYPYSLSGGQKQRVALARAMMIDPQIIGYDEP
                     TSALDPELRQTVEALIVQNREMGITQIVVTHDLVFAEAISDRIIRVNPK"
     gene            3900..4736
                     /locus_tag="VGT87_000152"
     CDS             3900..4736
                     /locus_tag="VGT87_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEQ3813044.1"
                     /translation="MIIKKRTVAILAIASSFFLVGCQATKSLKSGDAWGVYQKQKSIT
                     VGFDNTFVPMGYKDESGKCKGFDIDLAKEVFHQYGLKVNFQAINWDMKEAELNNGKID
                     VIWNGYSITKERQDKVAFTDSYMRNEQIIVVKKRSDIKTISDMKHKVLGAQSASSGYD
                     SLLRTPKLLKDFIKNKDANQYETFTQAFIDLKSDRIDGILIDKVYANYYLAKEGQLEN
                     YRMIPTTFENEAFSVGLRKEDKTLQAKINRAFRVLYQNGKFQAISEKWFGDDVATANI
                     KS"
     gene            5106..5879
                     /gene="cfa"
                     /locus_tag="VGT87_000153"
     CDS             5106..5879
                     /gene="cfa"
                     /locus_tag="VGT87_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_149017463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAMP factor pore-forming toxin Cfa"
                     /protein_id="HEQ3813045.1"
                     /translation="MKPKHLLCLSTVVAGLALFSTMTYSVLADDASNPDTILMNNNQA
                     NLQRDALVQKLDEGHQQLEAIKHEAKGTDIEATVNKAIDAVDHMKSSIRFNTETIYDF
                     SSIGARVEALSDAIKAIVFSTTQLTHKVEKAHTDMGFAITKLVIRIIDPFASVDAIKA
                     QVQEIKALEEKVINYPDLQPTDRATIYTKAKLNKAIWNTRLERDKKVLGIKPFDVYNR
                     LNKAITHAVGVQLNPTTTVQQVDDEVIAVQNALETALKS"
     gene            complement(6249..7484)
                     /locus_tag="VGT87_000154"
     CDS             complement(6249..7484)
                     /locus_tag="VGT87_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEQ3813046.1"
                     /translation="MTQDPIANLKLARKGPIVSIIVYLSLSVAKLLAGYLLNASSLIA
                     DGFNNLSDIVGNVALLIGLHLASQPADANHKFGHWKIEDLSSLVTSFIMFLVGFQVLI
                     HTIKSIFSGQQVDIDPLGAVVGIVSAFVMLGVYVFNKRLSKRVKSSALVAASKDNLAD
                     AVTSIGTSIAIIAASLHLPVIDHIAAMIITFFILKTAFDIFMESSFSLSDGFDSRHLK
                     KYEKAILEIPKIVAVKSQRARTYGSNVYLDIVLEMNPDLSVYESHSITEKVEQLLSDQ
                     FSIYDIDIHVEPAMIPEEELFDNVAKKLYRYEKLILSKVPDYDHYIAKSFQLIDANGQ
                     TFNYEQFLNQEIYYPSNFNHFQIESISQKTMLVTYQLNGNQRTSIWRRHESWSLLFHQ
                     ITPIAKRQLHHTHYRIVKM"
     gene            complement(7604..8926)
                     /locus_tag="VGT87_000155"
     CDS             complement(7604..8926)
                     /locus_tag="VGT87_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEQ3813047.1"
                     /translation="MIAFVKLIDNLVWGPPLLILLVGTGIYLTSRLGLIQILKLPRAF
                     KLIFSDDEGHGDISSFAALATALAATVGTGNIVGVATAIKSGGPGALFWMWVAAFFGM
                     ATKYAEGVLAIKYRTKDANGHISGGPMYYIVNGMGTKWKPLAILFAGSGILVALFGIG
                     TFAQVNSITSSLGHSFGLSPQMVSIVLAIFVAAIIFGGIHSISKVAEKVVPFMAIFYI
                     LSSLAVIFSHYQQLLPVIRLVFQSAFTPTAAIGGFAGSLMKDAIQKGIARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTIIICTLTGLSILVTGQWTGQLEGAPLT
                     QSAFATVFGNLGTFGLTFSLVLFAFTTILGWSYYGERCFEFLFGITHLTYFRIVFILM
                     VGLGGFLKLELIWVLADIVNGLMALPNLIALLALSPVVILETKHYFIK"
     regulatory      complement(9054..9140)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            9455..11773
                     /gene="pcrA"
                     /locus_tag="VGT87_000156"
     CDS             9455..11773
                     /gene="pcrA"
                     /locus_tag="VGT87_000156"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEQ3813048.1"
                     /translation="MNPLLNGMNDRQAQAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKFVNPWNILAITFTNKAAREMKERALSLNPATKDTLIATFHSMCVRILRREADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNIDPKKWNERSILGTISNAKNDLLDEKGYEAQA
                     ADMYSQIVARCYKAYQEELRRSEALDFDDLIMMTLRLFDANPDVLAYYQQRYQYIHVD
                     EYQDTNHAQYQLIKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPDAKVVL
                     LEENYRSTKKILQAANDVINNNRNRRPKKLWTQNADGEQLVYYRANDERDEAVFVAST
                     ISNMSQELGKNFKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDLIA
                     YLTIVANPADNISFERIVNEPKRGVGPGTLDKLRQFAYESDQSLLEAASNLLMSPLKG
                     KAAQAIMDLANILGQLRQDLDQMSITDLAEALLEKTGYLDSLRLQNTLESQARIENIE
                     EFLSVTKNFDESSASQEEDETGVDRLGRFLNDLALIADTDDSQAEAAEVTLMTLHAAK
                     GLEFPVVFLIGMEEGVFPLSRASEDPDELEEERRLAYVGITRAEEVLFMTNANTRTLF
                     GKSSYNRPTRFLKEISEELLSYKGLARPAQSSFGVRFSTETHTQFGQGMSLSEALQAR
                     KAQAQVRQSAQPMQAHTIPSASTSSVLPFGSNSSVEEVTWQIGDIAHHKKWGDGTVLE
                     VSGSGKTMELKIKFPEVGLKKLLASVAPIEKK"
     gene            12135..12383
                     /locus_tag="VGT87_000157"
     CDS             12135..12383
                     /locus_tag="VGT87_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEQ3813049.1"
                     /translation="MGLIWTLIVGALIGVIAGALTKKGGSMGWIANIAAGLVGSSVGQ
                     ALLGSWGPSLAGMSLIPSVIGAVIVVMITSFVLNKTNN"
     gene            12420..13031
                     /gene="amaP"
                     /locus_tag="VGT87_000158"
     CDS             12420..13031
                     /gene="amaP"
                     /locus_tag="VGT87_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEQ3813050.1"
                     /translation="MLSRKEVCMSKLLKISYCLVGLVLLSVFGWVVGITGGYIYLPYS
                     YRWLSWGMDSFPNLLDSALSYYYFWTALVLFVITFLALLVIILYPRIYTEVQLRHKNK
                     KGTLLLKKSAIESYVATAIQTAGLMPNPTVTAKLYKRKFKIIVKGRLASRVAVADQIS
                     GVKEGIEKGLTEFFGINYPVNFKVYVKDIADSDRKHITRNRVE"
     gene            13042..13230
                     /locus_tag="VGT87_000159"
     CDS             13042..13230
                     /locus_tag="VGT87_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEQ3813051.1"
                     /translation="MEFYEKFKYPIIGGLVGLILAILLMAFGLFKTLLAIIFIILGIY
                     GGLYAKKTGIIDQFLNRK"
     gene            13243..13782
                     /locus_tag="VGT87_000160"
     CDS             13243..13782
                     /locus_tag="VGT87_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEQ3813052.1"
                     /translation="MTETYIKNTSKDLTSAIRGQLTYDDKVIEKIVGLALENVDGLLG
                     VNGGFFANLKDKLVNTESVRDGVNVEVGKKQVAVDLDIVAEYQKHVPTIYDSIKSIVE
                     EEVKRMTDLDVIEVNVKVVDIKTKEQFEAEKVSLQDKVSDMARSTSEFTSHQVENVKA
                     SVDNGVEKLQDQKAEPRVK"
     gene            13823..14023
                     /locus_tag="VGT87_000161"
     CDS             13823..14023
                     /locus_tag="VGT87_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEQ3813053.1"
                     /translation="MSDEKYNAKLDQAGGKLKEGFGKISGDKSLETEGKVDKVTGKVK
                     EVIADAKDTVKGLAKGLDNKDK"
     gene            14034..14522
                     /locus_tag="VGT87_000162"
     CDS             14034..14522
                     /locus_tag="VGT87_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEQ3813054.1"
                     /translation="MEKTQIKSTLTYDDKVIEKIVGHALENVGGLLAVTGGFFSNIKN
                     NLVNSESVTDGVSVEVGSKEVAVDLAIIVEYGKDIPAIAESIKAIVSQNVDSMTHLKV
                     VEVNVNVVDIRTKEEHEAASVTVQDRVTSAASSTSQFVSEQTEKLKDTISDTVNSDEA
                     AK"
     gene            14685..15326
                     /locus_tag="VGT87_000163"
     CDS             14685..15326
                     /locus_tag="VGT87_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ3813055.1"
                     /translation="MISPKKEITSSKYQKIAISVAQRIANGEYEVGEKLKSRTTIAST
                     FNVSPETARKGLNILADLKILTLKHGSGAIVLSKERAIEFINQYESTHSIAVLKEKIR
                     ETINDQGKAMEKMAVLVNDFLMQSQSVSKQYPLAPYEIICSQDSEHFGKSIGVLNIWH
                     QTGATIVAIEHAGQFIVSPGPYSVIEKGDHIYFVGDESVISRMKTFFNLRKGL"
     gene            complement(15469..16011)
                     /locus_tag="VGT87_000164"
     CDS             complement(15469..16011)
                     /locus_tag="VGT87_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEQ3813056.1"
                     /translation="MTKRHTETKAYVKTALTTLLTEQSFETLTVSDLTKKAGINRGTF
                     YLHYTDKFDMMNHFKNDTLDDLYRLLNQAEIYTDTRQVLNQTLSYLIEHREFITALAT
                     ISYLKFPQLIKDFCYQFLTTITGFQDIVTNQYHIPYPYALEVYLASIESIISYWIANG
                     YQETTEEVADIILKAVTLDN"
     gene            16129..16830
                     /locus_tag="VGT87_000165"
     CDS             16129..16830
                     /locus_tag="VGT87_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ3813057.1"
                     /translation="MAFIELKQVSKSYQVGETTVFANHEVSFEINKGELVVILGASGA
                     GKSTVLNILGGMDTVDAGQVIIDGKDIAHYTSKALTQYRRNAIGFVFQFYNLVPNLTA
                     KENVELAVEIVADALDPVTILKEVGLSHRLDHFPAQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDYQTGKQILTLLQDMARTKGTTVVIVTHNAAIAPIADRVIFMHDAQVTK
                     TVINKEPASIETIDY"
     gene            16845..19481
                     /locus_tag="VGT87_000166"
     CDS             16845..19481
                     /locus_tag="VGT87_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEQ3813058.1"
                     /translation="MIKKTLWKDILRAIKNSKGRFISLFFLMALGSFALVGLKVTGPD
                     MERTASRYLERHQVMDLTVLASHQFSQADKQELDTLKGAHLEYGHLLDVSLTSNQKSL
                     RLYSVPKKVSKPVLVKGSWPKRETDLVLSSSLAKNYQIGDELVVTSPMEGLLTTTHFQ
                     VVGFANSSEVWSKSNLGSSSTGDGSLYAYAFVNPNVFKSAFNLLRIRFSNLRLTNAFS
                     KDYQKRVTQNQAHLDNLLKDNGQKRYDDLQNQYDLALKNGRAALAKETVKLAASEENL
                     TFLEGSALQEAKHQIEQGKQALAKEEKQLEQVQATKDKLEKPSYLTYNRSTLPGGEGY
                     HTYATSTTSISNVGNIFPVVLYLVAALVAFTTMTRYVDEERTSSGLLKAIGYSNKDIS
                     LKFLIYGLLASFLGTTLGIIGGTYLLSALISEILTGALTIGKTHLYSYWFYNGIAYLL
                     AMLSAVLPAYLIVKKELFLNAAQLLLPKPPSKGAKIWLEHLTFVWKALSFTHKVTIRN
                     IFRYKQRMLMTIVGVAGSVALLFAGLGIQSSLAKVVEHQFGDLTTYDILAVGSAKATA
                     TEQTDLASYLKQEPITGYQKVSYASLTLPVKGLPDKQSISILSSSTTSLSPYFNLLDC
                     QEQKKVPIPTSGVLISEKLASYYKVKPGDQLVLTDRKGQSYKVTIKQVIDMTVGHYLI
                     MSNTYFRNHFKGLEAAPAYLIKVKDKDSKHIKETASDLLTLKAIRAVSQNVNHIKSVQ
                     LVVTSLNQVMTLLVFLSILLAIVILYNLTTINIAERIRELSTIKVLGFYDQEVTLYIY
                     RETISLSLVGILLGIYLGKGLHTYIMTMISTGDIQFGVKVDAYVYLVPILVILSLLAV
                     LGIWVNRHLKKVDMLEALKSID"
     gene            19573..20169
                     /locus_tag="VGT87_000167"
     CDS             19573..20169
                     /locus_tag="VGT87_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEQ3813059.1"
                     /translation="MENSIEAYLADSREKVTLGTVVDCFKGKAVSSKVVPGDVGLINL
                     SDMGTLGIQYHQLRTFQMDRRQLLRYLLEDGDVLIASKGTLKKVCVFHKQNRDVVASS
                     NITVLRPQKLLRGYYIKFFLDLPIGQALLDAADHGKDVINLSTKELLDIPIPVIPLVK
                     QDYLINHYLRGLTDYHRKLNRAEQEWEYIQNEIQKGLG"
     gene            20180..21136
                     /locus_tag="VGT87_000168"
     CDS             20180..21136
                     /locus_tag="VGT87_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEQ3813060.1"
                     /translation="MGSVERTLKMTLATIVAILIAYQLHLDYAMSAGIIALLSVLDTR
                     KSSLVVARNRLLSFFLAFGIAMMCFSLFGFTTVGFMCYLLIIIPLLYHFQIEAGLVPI
                     TVLVTHLIAKKSIALPILSNEFMLFFVGTSVALLFNAYMGSQDQQIRYYHQKVESDLK
                     GILYRFESFLLEGKGQNEGLLIKNLDKILDEALKLVYRERHNQLFQQTNYQVHYFEMR
                     RQQNRLLGQMAINVNTLMRQSKESILLSHLFHETACQLSEQNPALTLIDDIEQLLETF
                     RHGDLPQTREEFERRAVLFQLLQDLERFILLKVEFYQDYQND"
     gene            21233..22573
                     /locus_tag="VGT87_000169"
     CDS             21233..22573
                     /locus_tag="VGT87_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEQ3813061.1"
                     /translation="MNEIKCPHCHTLFTINESEYSQLLEQVRGQAFDEELQKRLINEI
                     ALLEEKAKHQLHEVVAKKETAITSLTNQLEQIEKEQAYLRQEELAKKDQLIASLEAKL
                     DKLASQNALELANQLAEKDKEVVSLTNQLDKLALEKDATFQSKLATIEKERDGIKNQL
                     ALQAKESELSLASVRSDYEAQLKAANEQVEFYKNFKSQQSTKAIGESLELYAETEFNK
                     VRSYAFPNASFAKDNQLSSRGSKGDYIYREVDANGVEILSIMFEMKNEADTTKTKHKN
                     SDFFKELDKDRREKGCEYAVLVSMLEADNDYYNTGIVDVSHEYQKMYVVRPQLFIQLI
                     GILRNAAMNSLHYKQELALIKEQNIDITHFEEDLDQFKNAFAKNYQSASNNFKKAIDE
                     IDKSIKRMEEVKRFLTTSENQLRLANNKLEDVSVKKLTRQNPTMREKFEALKDQ"
     gene            22689..23573
                     /gene="truB"
                     /locus_tag="VGT87_000170"
     CDS             22689..23573
                     /gene="truB"
                     /locus_tag="VGT87_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEQ3813062.1"
                     /translation="MINGIINLKKEAGMTSHDAVFKLRKLLQEKKIGHGGTLDPDVVG
                     VLPIAVGKATRVIEYMTEAGKVYEGQVTLGYSTTTEDASGEVVARSSLPAVLTEELVD
                     QTMTTFLGKITQTPPMYSAVKVNGRKLYEYARAGESVERPRREVTISLFERTSPLNFT
                     EDGLCRFSFKVACSKGTYVRTLAVDLGRALGVESHMSFLQRSASAGLTLETAYTLGEI
                     ADMVSKQEMSFLLPIEYGVADLPKMVIDDTELTEISFGRRLSLPSQEPLLAAFHGEKV
                     IAILEKRDQEYKPKKVLI"
     gene            23602..24534
                     /locus_tag="VGT87_000171"
     CDS             23602..24534
                     /locus_tag="VGT87_000171"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEQ3813063.1"
                     /translation="MEIEYIKDYRDINQEDDTVLVLGYFDGLHRGHKALFDKAREVAN
                     KEGLKVVVFTFTESPKLAFLRFSPELLLHITYPKKRYEKFADYGVNKLYLVDFTSKFS
                     KVSSDHFITHYIKNLKAKHIVVGFDYKFGHNCTDSDYLTRNFEGQVYTIEEIKEDHRK
                     ISATWIRKLIQEGNVVKANHLLGYDLSTRGRVVHGDARGRTIGFPTANLAPIDNTYLP
                     ADGVYVTNVIVANKIYRSMTSLGKNVTFGGKELRLEVNIFDFDEEIYGEIIEIVWLDK
                     IRDMEKFEGIEDLTDRLEYDKRTALNWKKDSKLS"
     gene            24577..24981
                     /locus_tag="VGT87_000172"
     CDS             24577..24981
                     /locus_tag="VGT87_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEQ3813064.1"
                     /translation="MVTLFLSPSCTSCRKARAWLVKHEVDFQEHNIITSPLSRDELMS
                     ILSFTENGTEDIISTRSKVFQKLDVDVEELSISDLIDLIAKNPSLLRRPIIMDQKRMQ
                     IGFNEDEIRAFLSRDYRKQELRQATIKAEIEG"
     gene            24983..25261
                     /locus_tag="VGT87_000173"
     CDS             24983..25261
                     /locus_tag="VGT87_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEQ3813065.1"
                     /translation="MSGNYYYPLDLSWSTEEISSVLHFLNKVELAYEKKVDAKQLLNS
                     YKTYKTIVKSKAQEKQIDRDFQKVSGYSTYQVVKKAKAIEKGFFSLGN"
     gene            25251..26039
                     /locus_tag="VGT87_000174"
     CDS             25251..26039
                     /locus_tag="VGT87_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEQ3813066.1"
                     /translation="METKYAFARQIIKEAGLFIKSKMSEQLDIQVKTQFDDLVTNVDQ
                     ETQQLLMDRIHQTYPCDAILAEENDVRHPINQGNVWVIDPIDGTVNFIVQGSQFAVMI
                     AYYEQGIGQFGLIYDVMADQLLAGGGDFEVTLNGDKLPAYQEKPLERSLIGCNAGMFA
                     RNDRNLAHLIAKTLGVRVYGGAGICMVKVMKQELLAYFSFIQPWDYAAAKVLGDKLGY
                     VLLTIDGYEPDFQTRQKIMFVPKCQLTRIASFLTKDSAVTLEDQ"
     gene            26042..27352
                     /locus_tag="VGT87_000175"
     CDS             26042..27352
                     /locus_tag="VGT87_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEQ3813067.1"
                     /translation="MMSLPKEFINTYQAILGKELEDFLASFNQEPVNAFRINPLKNQL
                     KTFEHPIPNTLWGYYGKLSGKSPEHVSGLVYSQEPAAQMVAQVAAPQKGSRVLDLAAA
                     PGGKSTHLLAYLDNTGLLVSNEISKKRSKVLVENIERFGARNVVVTNESADRLAKVFS
                     HYFDTIVFDGPCSGEGMFRKDPDAIQYWHHGYPAECAKLQKSILEDALAMLKPGGELI
                     YSTCTWAPEENEDVVQWLLETYTFLELVDVPKLNGMVSGIGLPETARMYPHRYQGEGQ
                     FVAKLKDKRQEGQSTKLKAPKSNLIKDQLRLWKMFEKDHINLTLSGTLQTFGDYLYLL
                     PDGLPALDGLKIARNGLELGVFKKKRFEPSYALGLALKPDEVRSSIELREEDFRPYVS
                     GNVITLPQSYPTGWYQLLINGNGLGFAKVVDKTIKNNFPKGLRF"
     gene            27491..28357
                     /locus_tag="VGT87_000176"
     CDS             27491..28357
                     /locus_tag="VGT87_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEQ3813068.1"
                     /translation="MKMKKKFFLLSLLVLSTFFLSACSSWIDKGESITAVGSTALQPL
                     VEAVADEFGSSNLGKTVNVQGGGSGTGLSQVQSGAVQIGNSDVFAEEKDGIDASKLVD
                     HQVAVAGLAVIANPKVKVSNLSSQQLQKIFSGEYTNWKQVGGEDLAISVINRAASSGS
                     RATFDSVIMKGVNAKQSQEQDSNGMVKSIVSQTPGAISYLSFAYVDSSVKSLQLNGFK
                     ANAKNVATNDWPIWSYEHMYTKDKPTGLTKEFLDYMFSDEVQQNIVTHMGYISINDME
                     VVKSHDGKVTKR"
     gene            28368..29303
                     /gene="pstC"
                     /locus_tag="VGT87_000177"
     CDS             28368..29303
                     /gene="pstC"
                     /locus_tag="VGT87_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEQ3813069.1"
                     /translation="MENQELAKKLASPSKNSRLETFGRTITFLCLALIVFIVAMILIF
                     VAQKGLSTFFIDKVNLFDFLFGKEWQPSVKNAAGIPYLGALPMITGSFLVTVLSAIIA
                     TPFAIGAAVFMTEISPKYGAKLLQPAVELLVGIPSVVYGFIGLQVIVPFMRSIFGGTG
                     FGILSGVCVLFVMILPTVTFMTTDSLRAVPRHYREASMAMGATRWQTIWRVVLNAARP
                     GIFTAVIFGMARAFGEALAIQMVVGNSAVMPSSLTTPAATLTSVLTMGIGNTVMGTVQ
                     NNVLWSLALVLLLMSLAFNSLVKLITKERKRNYER"
     gene            29293..30180
                     /gene="pstA"
                     /locus_tag="VGT87_000178"
     CDS             29293..30180
                     /gene="pstA"
                     /locus_tag="VGT87_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEQ3813070.1"
                     /translation="MNAKKVDKVATGTLYTIAGIIVAILASLILYILVRGLPHISWSF
                     LTGKSSSYEAGGGIGIQLYNSFFLLIVTLIISIPLSTGAGIYLAEYAKKGPVTNFIRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLLHVH
                     HTQREAGLALGLSRWETVFYVVIPEALPGMVTGIVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNPLSVTSPISIFRQSETLAVHIWKVNSEGTIPDATLVSAGSAAVLLIFILIF
                     NFSARFIGKKLHSKMTAAK"
     gene            30196..30999
                     /gene="pstB"
                     /locus_tag="VGT87_000179"
     CDS             30196..30999
                     /gene="pstB"
                     /locus_tag="VGT87_000179"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEQ3813071.1"
                     /translation="MTEYNWNERHIITFPEETLALATKDLHVYYGAKEAIKGIDMQFE
                     KHKITALIGPSGCGKSTYLRSLNRMNDTIDIARVTGEILYQGIDVNRKDMNVYEIRKH
                     LGMVFQRPNPFAKSIYKNITFAHERAGVKDKKVLDEIVETSLKQAALWDQVKDDLHKS
                     AFTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNYTIIIV
                     THNMQQAARASDYTAFFYLGNLIEYDKTRNIFQNAQCQSTNDYVSGHFG"
     gene            31012..31770
                     /gene="pstB"
                     /locus_tag="VGT87_000180"
     CDS             31012..31770
                     /gene="pstB"
                     /locus_tag="VGT87_000180"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEQ3813072.1"
                     /translation="MTEPILQIRDLSVYYNQKKTLKDVSLDLYPNEITALIGPSGSGK
                     STLLRSINRMNDLNPEVTITGSIVYNGHNIYSPRTDTVDLRKEIGMVFQQPNPFPMSI
                     YENVVYGLRLKGIRDKSILDHAVESSLKGASIWNEVKDRLHDSAVGLSGGQQQRVCIA
                     RVLATSPRIILLDEPTSALDPISAGKIEETLLLLKKDYTLAIVTRSMQQASRLSDRTG
                     FFLEGDLLECGPTKAMFMNPKRKETEDYISGKFG"
     gene            31838..32491
                     /gene="phoU"
                     /locus_tag="VGT87_000181"
     CDS             31838..32491
                     /gene="phoU"
                     /locus_tag="VGT87_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEQ3813073.1"
                     /translation="MLRTKFEEELDKLHNQFYSMGTEVLAQINKTVRAFVSHDRELAK
                     EVIEEDDTINNFETKLEKKSLEIIALQQPVSNDLRMVITVLKASSDIERMGDHAASIA
                     KATIRMKGEERIPVVEEQINLMGKAVKQMVEEALNAYINADDTKAYEIAASDEIIDQY
                     FRNIQTLAVEEIRKSPDAVFAGKEYFQVLMYLERIGDYARNICEWIVYLKTGKIIEL"
     gene            32696..35233
                     /locus_tag="VGT87_000182"
     CDS             32696..35233
                     /locus_tag="VGT87_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEQ3813074.1"
                     /translation="MKTVEHLIETFVPENYNIFLDINRQTKTFTGNVAINGEALDNHV
                     AFHQKDLDIKSILLDNEAVIYQVDNDNEVVRVELPETGMMTLVIEFSGSITDNMTGIY
                     PSYYTKNGEKKEVISTQFESHFAREAFPCIDEPQAKATFDLSLTFDQEIGEIALSNMP
                     EVNIDRREETGLWTFDTTPRMSSYLLAFALGELHGKTVESKKGTTVGVYATTAHPLSS
                     LDFSLDIAVRVINFYEDYFGVHYPIPQSLNIALPDFSSGAMENWGLITYREIYLLVDE
                     NSTVQSRQQVALVIAHEIAHQWFGNLVTMKWWDDLWLNESFANMMEYVSIEAIEPSWK
                     IIEDFQTGGIPLALKRDATDGVQSVHVEVNHPDEINTLFDPAIVYAKGSRLMHMLRRF
                     IGDRDFAIGLHHYFEKYQYRNTVGRDLWNILSDTSGKDVAAFMDAWLEQPGYPVLTAR
                     LENDQLILSQKQFFIGKGEEKGRLWPIPLNTNWHGLPETLTEAEMVIPNFSQLAAENE
                     GALRFNIDNTAHYITDYQGSLLDALVTELAQLDNTSALQVIQERRLLADSGLISYAEL
                     VDLIAQLDDSKSYMVAEAVQQVVSGLKRFIDEGSLAEKSFNRLVTTIYQEDFNQHGFE
                     KKADESDEDEMVRQVALGRLWLAENQTIIDGLRTIFEAYQNNIASIPAAVRRLVLANQ
                     MKYFETDSLVDIYFETYVATTDNNLRSDLTVAFSQTKQSTTIRRILVSLKDKDIIKPQ
                     DLSYWYNALLGQSFTQDIIWEWARENWDWIKSALGGDMSFDKFVIYPASNFKTPKHLA
                     EYKSFFEPKLDDMAISRNITMGINEIEARVALITKEKEAVIAALSHY"
     gene            35580..36254
                     /locus_tag="VGT87_000183"
     CDS             35580..36254
                     /locus_tag="VGT87_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ3813075.1"
                     /translation="MIKILLVEDDLSLSNSIFDFLDDFADVMQVFDGDEGLYEAESGI
                     YDLILLDLMLPEKNGFQVLKELREKDIKIPVLIMTAKEGLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRTGKFADKNISFGNLVVDLARKEVKVEGKVVELLGKEFDLLVY
                     LLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYISKIRKKLKGTRFVNRLQTLRSVGY
                     ILKNNE"
     gene            36247..37557
                     /locus_tag="VGT87_000184"
     CDS             36247..37557
                     /locus_tag="VGT87_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEQ3813076.1"
                     /translation="MNKLKKEILSDNYNHFFHFFAVFTGIFVIMTIIILQIMRFGVYS
                     SVDSSLVSVSNNASSYANRTMARISSFYFDTENNIIKALPDSDSSKLLGTPAANTDII
                     LFSANGTILNAFDAFSNYQNFHLDKRRLGSIETTSLMNFYGQEEKYHTITVRVHIKNY
                     PAVAYMMAVVNVEQLDRANERYERIIIIVMSVFWLISILASIYLAKWSRKPILESYEK
                     QKMFVENASHELRTPLAVLQNRLESLFRKPNETILENSEHLASSLDEVRNMRILTTNL
                     LNLARRDDGINPQWTHLDTDFFNAIFENYELVAKEYGKIFYFQNQVNRSLRMDKALLK
                     QLITILFDNAIKYTDKNGIIEIIVKTTDKNLLISVIDNGPGITDEEKKKIFDRFYRVD
                     KARTRQTGGFGLGLALAQQIVMSLKGNITVKDNDPKGSIFEVKL"
     gene            complement(37682..37930)
                     /locus_tag="VGT87_000185"
     CDS             complement(37682..37930)
                     /locus_tag="VGT87_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEQ3813077.1"
                     /translation="MEVKTLANIKSAIKRAELNVKANEKNSAQKSAMRTAIKAFEANP
                     SEELFRAASSSIDKAESKGLIHKNKASRDKARLAAKLG"
     gene            complement(37984..38904)
                     /locus_tag="VGT87_000186"
     CDS             complement(37984..38904)
                     /locus_tag="VGT87_000186"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEQ3813078.1"
                     /translation="MSNEFINFEKISRESWKTLHQKAKALLTQEELKSITSLNDNISI
                     NDVIDIYLPLINLIQVYKIAQENLSFSKSLFLKKDIQLRPFIIGISGSVAVGKSTTSR
                     LLQLLLSRTHPNSQVELVTTDGFLYPNQFLIEQGLLNRKGFPESYNMELLLDFLDSIK
                     NGQTAFAPVYSHDIYDIIPNKKQSFNNPDFLIVEGINVFQNQQNNRLYMSDYFDFSIY
                     IDADSSHIETWYIERFLSILKLAKRDPHNYYAQYAQLPRSEAIAFARNVWKTVNLENL
                     EKFIEPTRNRAELILHKSADHKIDEIYLKK"
     gene            39139..39765
                     /locus_tag="VGT87_000187"
     CDS             39139..39765
                     /locus_tag="VGT87_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEQ3813079.1"
                     /translation="MEKYLKKIGKMMTKMYYDENPDSLHDIHEVKVELLNHPFTFLTD
                     SGVFSKKMVDFGSQVLLETLNFKENERVLDLGCGYGPLGISLAKVQRVDATLVDINNR
                     ALDLARKNATNNQVAVTIFQSNIYENISGHFEHIISNPPIRAGKRVVHSIIEKSIDFL
                     VVNGDLTIVIQKKQGAPSAKAKMATIFGNVEILRKDKGYYVLRSIKQA"
     gene            40418..>40800
                     /locus_tag="VGT87_000188"
     CDS             40418..>40800
                     /locus_tag="VGT87_000188"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEQ3813080.1"
                     /translation="MGTTDLVSCAVQASEYAYVPYSHFPVGAALKTKDGTIYTGCNIE
                     NVSFGLTNCGERTAIFKAISDGHKELVEIAIYGETMQPVSPCGACRQVMAEFFAPSSL
                     VTLIAKNGQTVEMTVGDLLPYSFTD"
BASE COUNT        13140 a         6759 c         8257 g        12644 t
ORIGIN      
        1 ataattagac tataccaatt ttatgtttga caagacattg tggatattat atactgtaaa
       61 catactgttt ttatcggtat aattagacta taccaattat tgaaattatg agagaaagta
      121 gcaagttaac gaacttgtta aggtaattaa gatgtgtgga attgttggag ttgttggaaa
      181 tcgcaatgca acggatattt taatgcaagg ccttgaaaag cttgaatacc ggggttatga
      241 ttcagcagga atttttgtgg ctaatgccaa tcaaacaaac ttgattaaat cagtggggcg
      301 gattgctgat ttgcgtgcca agattggcat tgatgttgct ggttcaacag ggattggtca
      361 cacccgttgg gcaacgcatg gccaatcaac agaggataat gcccatcctc acacgtcaca
      421 aactggacgt tttgtacttg ttcataatgg tgtgattgaa aattaccttc acattaaaac
      481 agagttccta gctggacatg attttaaggg gcagacagat actgagattg cagtacactt
      541 gattggaaaa tttgtggaag aagacaagtt gtcagtactg gaagctttta aaaaagcttt
      601 aagcattatt gaaggttcct atgcctttgc gttaatggat agccaagcaa ctgatactat
      661 ttatgtggct aaaaacaaat ctccattgtt gattggactt ggtgaaggtt acaacatggt
      721 ttgttcagat gccatggcca tgattcgtga aaccagtgaa tttatggaaa ttcatgataa
      781 ggagctagtt attttaacca aagataaggt aactgttaca gactacgatg gtaaagagct
      841 gatacgagat tcctacactg ctgaattaga cttatctgat attggcaaag ggacttatcc
      901 tttctatatg ctgaaagaaa ttgatgagca accaaccgta atgcgtcaat taatttcaac
      961 ttatgcagat gaaactggta acgtacaggt tgatccagct atcattacct ctatccaaga
     1021 ggctgaccgt ctttatattt tagcggcagg gacttcctac catgctggtt ttgcaacaaa
     1081 aaatatgctt gagcaattga cagatacacc agttgagttg ggagtggctt ctgagtgggg
     1141 ttaccacatg cctctgctta gcaagaaacc aatgtttatt ctactaagcc aatcaggaga
     1201 aaccgcagat agtcgtcaag ttttagtaaa ggcaaatgct atgggcattc cgagtttgac
     1261 agtaactaac gttccaggat caaccttatc acgtgaatca acatacacca tgttgattca
     1321 tgctggacct gaaattgctg ttgcatctac aaaagcttac actgcacaaa ttgctgccct
     1381 tgcctttttg gctaaggcag ttggtgaggc aaatggtaag caagaagctc ttgactttaa
     1441 cttggtacat gagttgtcat tggttgccca atctattgag gcgactttgt ctgaaaaaga
     1501 tctcgtggca gaaaaggttc aagctttgct aactactact cgtaatgctt tttacatcgg
     1561 gcgtggcaat gattattacg ttgcgatgga agctgctttg aaattaaaag agatttctta
     1621 tattcaatgc gaaggctttg cggctggtga attgaaacat ggaaccattt cattaattga
     1681 ggaggacacg ccagtaatcg ctttaatatc gtctagtcag ttggttgcct ctcatacgcg
     1741 tggtaatatt caagaagttg ctgcccgtgg ggctcatgtt ttaacagttg tggaagaagg
     1801 gcttgaccgt gagggagatg acattattgt caataaggtt catcctttcc tagccccgat
     1861 tgctatggtc attccaactc aactgattgc ttactacgct tcattacaac gtggacttga
     1921 tgttgataag ccacgtaatt tggctaaagc tgtaacagta gaataaatga ttataatgga
     1981 taaggcaacc atctgttagg tggttgcttt ttgtttttag gagaatagca gagcttagaa
     2041 aatggccttg agaaaagcta tacaagaaga atagtcatct tctgatgaga acgtattcat
     2101 cttgtcaaat ccttgattct aaagtaaaat ggtaacatta agaaatgttt aggagtgtaa
     2161 gatgtcatta ccaaattgtt tgcaatgcca gtctgaatat gtttacgaag atggtctttt
     2221 attaatctgt ccaatgtgtg cttttgaatg gacaccaggc gaagaagcga ctgaagaaga
     2281 aggacttgtt gttttagata gcaatggtgt tcgattatct gatggcgata ctattactgt
     2341 ggttaaagat ttgaaagtaa aaggcgctcc aaaagacctg aaacaaggga ctcgtgttaa
     2401 aaatattcgc ctggtggaag gtgatcataa tattgattgt aagattgatg ggtttggcgc
     2461 gatgaaattg aaatcagaat ttgtcaagaa aatctaataa taatacgcaa ccagctgatc
     2521 tggttgtatt tttataaaaa aacctctccc caaatacgtt caaatttggt ataatagtaa
     2581 aatattcagt tttaggaggc aacatgacct atattcagca agttttaccc agcttattag
     2641 atggtgcctt ggtgacctta caagtattct ttattgttat cattctttct atacccttag
     2701 gggctatttt agctttcttg atgaagattc cctttaaacc gctacagtgg tttttgaccc
     2761 tatacgtgtg gatgatgcga gggacaccat tactacttca attgattttt ttctattatg
     2821 ttttgccaag tgtggggatt agttttgatc gaatgccagc tgctattttg gcgtttactt
     2881 tgaattatgc tgcctacttt gctgaaattt ttagaggtgg gattgaggct attccaaaag
     2941 gtcaatatga agcagctaaa gtattaaagt taaaacctct tcaaaccatt cggtatatta
     3001 ttttgcctca agtgtttaaa attgtgttac caagtgtttt caatgaagtc attaatttgg
     3061 tcaaagattc ttcccttgtc tatgtactcg gtgtaggaga tcttttatta gcaagtaaga
     3121 cggcagccaa tagggatgca accttagccc ctatgtttat cgctggtctt atctatttgc
     3181 ttttaattgg actggtcacg attatttcaa aacaagttga aaaacggttt aattattatc
     3241 agtaaggagg cttcatgtta gaactcaaaa atatttccaa acagtttggt caaaaaacca
     3301 tttttgatgg ctttaatcta accgttcaag acggagaagt gctctctttg gtaggcccat
     3361 caggtggcgg taagacaaca ctcttacgga tgttggctgg tcttgaatct attgattcag
     3421 gtcaagtctt ttacaatggc gaagatgttg gtattgatca tttggaaaat cgtaacttgc
     3481 ttggatttgt tttccaagat tttcaattgt ttcctcatct taccgtttta gacaacctaa
     3541 cgctgtctcc aacaattaca atgggcaagc aaaaggctga cgctaaagaa aaggctttgg
     3601 atttactggc acgtttagga ttaaaagaac atgcccaagt ttatccctat tcgttatctg
     3661 gaggtcaaaa acaacgggtt gctttggcaa gagccatgat gattgaccct caaattatcg
     3721 gttacgatga accgaccagt gctctagatc ctgaattacg tcagacggta gaagccttga
     3781 ttgttcaaaa ccgtgaaatg ggaataactc aaattgtagt cactcacgat cttgtttttg
     3841 ccgaagccat ctcagatcgt attattaggg ttaatcctaa atagaagagg acccaaaaga
     3901 tgattataaa aaaaagaacc gtagcaattt tagccatagc tagtagcttt ttcttggtag
     3961 gttgtcaagc tactaaaagt cttaaatcag gagatgcttg gggagtttac caaaagcaaa
     4021 aaagtattac agttggtttt gacaatacgt ttgttcctat gggctataag gatgaaagcg
     4081 gcaaatgcaa aggttttgat attgatttgg ctaaagaagt ttttcaccaa tatggactca
     4141 aggttaactt tcaagctatt aattgggaca tgaaagaagc agaactaaac aatggtaaaa
     4201 ttgatgtaat ctggaatggt tattcaataa ctaaggagcg tcaggataag gttgccttta
     4261 ctgattctta catgagaaat gaacaaatta ttgttgtcaa aaaaagatct gatatcaaaa
     4321 caatatcaga tatgaaacat aaagtgttag gagcacaatc agcttcatca ggttatgact
     4381 ccttgttaag aactcctaaa ctgctgaaag attttattaa aaataaagac gctaatcaat
     4441 atgaaacctt tacacaagct tttattgatt taaaatcaga tcgtatcgat ggaatattga
     4501 ttgacaaagt atatgccaat tactatttag caaaagaagg gcaattggag aattatcgga
     4561 tgatcccaac gacctttgaa aatgaagcat tttcggttgg acttagaaaa gaagacaaaa
     4621 cgttgcaagc aaaaattaat cgtgctttca gggtgcttta tcaaaatggc aaatttcaag
     4681 ctatttctga gaaatggttt ggagatgatg ttgccactgc caatattaaa tcttaaaaag
     4741 gacacttgtc ctttttttgt ttaacaggtg agatctagca gacaaagtca gctagacgat
     4801 ttaataaaaa tcttagttta aggtttttta ttaaactgta ttttttctaa aaattatagt
     4861 ttttttaaaa ctgataaatg acaagagaag ttattttagg tcaattaacg tccatttaaa
     4921 agtaatacta ggcatttaat gacattaaaa aatgattatt tttgtgaaat cctcttattt
     4981 tctggaaatt aaagaaatat tgaccttagt aaaaacaagt agtatgataa cgttataagc
     5041 gcttataaat taatttaact tcacttaact atttaaatag gtttattata ttcaggagga
     5101 ataatatgaa acccaaacac cttctctgtc tttcaacagt tgttgctgga ttagctttat
     5161 tttcaactat gacgtactca gttttagctg atgatgcttc aaatcccgat actattttaa
     5221 tgaataacaa ccaagcgaat cttcaaagag atgctctagt gcaaaagctt gatgagggac
     5281 atcaacaatt agaagctatt aaacatgaag ctaaaggtac tgatattgag gctactgtta
     5341 acaaagctat cgatgctgtt gatcacatga agagttctat acgcttcaat actgaaacga
     5401 tctatgattt tagttcaatt ggggcaagag tagaagcatt atcagatgct atcaaagcaa
     5461 tcgtattttc cacaacccaa ttaactcata aagtagaaaa agcgcatact gatatggggt
     5521 ttgctattac taaattagtc attcgtatta tagatccatt tgcatctgtt gacgctatca
     5581 aagctcaagt acaagaaatt aaagcacttg aggaaaaagt tatcaattat cctgatttac
     5641 agccaacaga ccgtgctact atctatacta aagctaaact aaataaagct atttggaaca
     5701 ctcgtttaga aagagataaa aaggttttgg ggatcaaacc atttgacgtt tacaatagac
     5761 ttaacaaagc tatcacacat gcggttggtg tccaactaaa tccgacaacc actgttcaac
     5821 aagttgacga tgaagttatt gctgtgcaaa atgctttgga aacagctctt aagtcataga
     5881 gtgtataagt aatgtgttgt ataggtagat gattacatta ggatatagaa taggtaaaga
     5941 ttttaaatat cttaccattg tagagtttga ggtgatgctc tcataaacaa tgagtcacct
     6001 gtagcccttt ttgcctttaa catgatgtac caatttaagg tattgttcaa ccgtcactat
     6061 tgaatcttgt ttaacctcct aaactaataa gattcaatat gaaaacgtgt attcttaaaa
     6121 aaatccctat gatctgttta aaaacaaatt gtagggattt ttttaactat ttaacttatc
     6181 attttgggac taggaatcgc tttaggaata tcatgaagta tagttatgtt ataagagaat
     6241 taatgttttt acatttttac aatacgatag tgtgtgtgat gtaattgtct cttagcgata
     6301 ggggtaattt ggtggaagag taaggaccaa gattcatgac gcctccaaat actggtacgt
     6361 tgattgccat ttaattggta agttaccaac atcgtttttt gactaatgga ttcaatctga
     6421 aaatggttga agttacttgg ataataaatt tcttgattca aaaattgttc atagttaaat
     6481 gtttggccat tcgcatcaat cagttggaaa gacttagcaa tgtagtggtc atagtcagga
     6541 accttactca aaattaattt ttcgtagcgg tagagctttt tggcgacatt atcaaaaagc
     6601 tcttcttcgg gaatcatggc aggctcaacg tgaatgtcaa tgtcataaat agaaaattgg
     6661 tcactcaata actgctccac tttctctgta atagagtgac tttcatagac tgaaagatca
     6721 ggattcattt caagtacaat atcaagatag acattgctac cataggtcct agctcgttga
     6781 gacttaacgg cgacaatttt agggatttct aaaatggctt tttcgtattt tttcaaatga
     6841 cggctatcaa atccatcaga taaactaaac gaactttcca taaagatatc aaaagctgtt
     6901 ttaagaataa agaacgtaat gatcatagca gctatatgat cgataactgg taaatgcaaa
     6961 gaagctgcta taatagcaat tgatgttccg atagaagtaa cagcatcagc tagattatcc
     7021 ttagaagcag cgactaaggc actggatttt acacgtttgg aaagacgttt gttaaagaca
     7081 tagaccccta acataacaaa agctgaaacg ataccgacaa cagccccaag agggtcaata
     7141 tcaacttgct gaccactaaa gatactttta attgtgtgaa tcagtacttg gaaacctaca
     7201 agaaacataa taaaagaagt gacaaggctg gataagtctt caattttcca atgaccaaat
     7261 ttatgattgg catcggctgg ttggctagct aagtgaagac caataagcag ggctacattt
     7321 cccacaatat ccgataaatt gttaaatcca tcagcaataa gggaacttgc atttaacaaa
     7381 taaccagcta ataatttggc aacactaagc gacaagtaga caataatact aacgattggg
     7441 ccttttctgg ctagttttaa atttgcaatc ggatcctgtg tcataataac tcctttgatt
     7501 tcaactttat attataccat aaaaaggggc ttaagaaaaa agcctagcgt cttgaacttg
     7561 gcagaagaag actaggtata actgggtagt actaacgtca tgtttactta ataaagtaat
     7621 gcttggtttc taaaataaca actggcgaga gagctaggag ggcaatcagg ttaggaagtg
     7681 ccattaaacc attgacaata tcagccaaaa cccaaattag ttctaactta aggaagcctc
     7741 ctaggcctac cattaagata aagacaatac ggaagtaggt gagatgtgta atgccaaaga
     7801 ggaattcaaa gcagcgttct ccataataac tccatcctaa aatcgttgta aaggcgaaga
     7861 gaactaggga aaaggttagg ccaaaagtcc ctaaatttcc aaacacagtg gcaaaggcag
     7921 attgggtcag aggagcccct tcgagctgcc cagtccattg gcctgttact aaaatagata
     7981 ggccggttag ggtacagata ataatcgtat caataaaagt tcctgtcatg gagatcagtc
     8041 cctgctccac gggttcattg gttttagctg ctgctgcagc aataggtgca gatcctagtc
     8101 cagactcatt ggagaagaca ccgcgtgcaa ttcctttttg aatggcatcc ttcatgagac
     8161 ttccagcaaa gcctccaata gcagctgtcg gagtgaaggc tgattgaaag actaaacgaa
     8221 taacaggaag tagctgctgg tagtgactaa aaataactgc taaactagat agaatataaa
     8281 aaatagccat aaaaggaacg actttttcag ccactttgga aatggaatga atgccaccaa
     8341 aaataatggc agccacaaag atagctaaaa caatactgac catttgtggg gataagccaa
     8401 agctatgacc taaagacgag gtgatcgagt tcacttgagc aaaggttcct attccaaata
     8461 gggcaactag gatccctgaa cccgcaaaaa gaatggctaa gggtttccat tttgtcccca
     8521 tgccattaac aatatagtac ataggaccac cagaaatatg accgttggca tcctttgtac
     8581 ggtatttaat cgctagtacc ccttcagcgt acttagtggc cattccaaaa aaagcggcaa
     8641 cccacatcca aaagagcgct ccaggaccac cagacttgat agcagtggca accccaacaa
     8701 tgttaccagt tccgacagta gcggcaaggg cagttgcaag agcagcaaag gatgaaatat
     8761 ctccatgtcc ttcgtcatct gaaaaaatga gtttaaaggc tcttggtagt tttaagattt
     8821 gaattaatcc taaacgactg gtaaggtaaa tccccgtccc aaccaataaa attaagaggg
     8881 gcggtcccca aacaaggtta tcaattaatt taacgaatgc tatcatcttt ctctcctaaa
     8941 agtcatcgaa gacgccaaaa gaaaaaagct atctctttct ttgagacaac ttctgaagca
     9001 atgataaaac agcaaacaca aaaaagacta agactgttct taatggtgtt tgtattttac
     9061 cttctgtcct tttgcctgag agattgagca gtatgccttg ccccttcggc gcctaataag
     9121 gtctctccag aagtccgtcc atcaaacagt cccgttcaaa aactgaacac ctgagagtat
     9181 aactccttcg gcgaaaaaaa ttcttctcct gcatgacatc attcggttta ttaaattagg
     9241 actatcatac ctcaaaaaac gaatgaagtc aaccaaaata gcaaaaaaat aaggaatgct
     9301 ttaagaaatc gctattattt ttaaatatct gatcctaaac tagaaaaaag gaatctcttt
     9361 acttttgggg tcttctttgc tatttttctt ggaaacatga tataatttta aggttgataa
     9421 aagagatgtc actgaacgag attaaggaga aagcatgaat cctttattaa atggaatgaa
     9481 tgatcggcag gcacaagctg ttcaaacaac tgaaggcccc ttattgatta tggcgggggc
     9541 tggttctggt aaaacaaggg tactgaccca tcgtattgcc tatttaattg atgaaaaatt
     9601 tgtcaatcct tggaatattt tagctattac ctttactaat aaggctgcgc gtgaaatgaa
     9661 agaaagagcc ttgtccttaa atccagctac taaagatacc ttaattgcta cctttcactc
     9721 catgtgtgta cggattctgc gtcgtgaggc tgatcacatt ggttacaacc gaaacttcac
     9781 aatcgtggat ccaggtgagc aacgaacgtt gatgaagcgc attttaaaac aattaaatat
     9841 agaccctaaa aagtggaatg aacgttctat tttaggaact atttcaaacg ctaaaaatga
     9901 tttgcttgat gaaaagggat acgaggcaca ggctgctgat atgtatagtc aaatcgtagc
     9961 gaggtgttac aaagcttatc aggaagaatt acgacgtagc gaagcacttg actttgatga
    10021 tttaatcatg atgacgctac gcctgtttga cgctaatcct gacgtgttag cttattacca
    10081 gcagcgttac caatacattc atgtggatga gtaccaagat accaaccatg ctcaatatca
    10141 gctaattaaa ttgctagctt ctcgctttaa aaatatttgc gttgttggag acgctgatca
    10201 gtctatctat ggttggcggg gagctgacat gcaaaatatt cttgattttg aaaaagatta
    10261 cccagatgct aaggtagtgc ttttagaaga aaactatcgc tctaccaaga aaattctgca
    10321 agcagctaac gacgtgatta acaataaccg taatcgccgt cctaagaaac tttggacaca
    10381 aaatgcggat ggggaacaac tggtttatta cagggcaaat gatgagcgag atgaggcagt
    10441 atttgtggcc tcaaccatta gcaatatgag tcaagagctt ggaaaaaact tcaaagattt
    10501 cgctgtttta taccgtacca atgcccaatc tcggaccatt gaagaagcac tcttaaaatc
    10561 caatattcct tacaccatgg ttggaggcac caagttttac agccgtaaag aaattcggga
    10621 tttgattgcc tatttaacca ttgttgctaa ccctgctgat aatatttctt ttgagcggat
    10681 tgtgaatgag cctaaacgag gtgttggtcc aggaacgctt gataaactgc gtcagtttgc
    10741 ctatgagagt gaccagtctt tattagaagc agcttcaaat ctattgatgt cccccttgaa
    10801 aggtaaagca gcacaagcta tcatggactt ggccaatatt ctcggtcagc tacgtcaaga
    10861 cctagatcag atgagtatca ccgacttagc agaagctttg ttagaaaaaa caggctattt
    10921 agatagctta cgcctgcaaa atacattgga aagccaagcc cgtatcgaaa atatcgaaga
    10981 attcttatct gttaccaaaa attttgacga gtcaagtgcc tctcaagaag aagacgaaac
    11041 cggtgttgat cgcttgggac gttttttaaa cgatttagca ctgattgctg atacagatga
    11101 ttcccaagca gaagctgctg aagtaacctt aatgacccta catgctgcca aaggcttgga
    11161 gttccctgtt gttttcttaa ttggtatgga agaaggtgtc ttccccttat cacgtgcctc
    11221 tgaagatcca gatgaattgg aagaagaaag acgtttagct tatgtcggaa tcacaagagc
    11281 tgaagaagtt ctctttatga ccaatgccaa caccagaacc ctctttggta aaagtagtta
    11341 taaccgtcca acacgcttct tgaaagaaat ttcagaggaa ctactctctt ataaaggatt
    11401 ggcaagacca gcacaatctt catttggtgt gcgtttttct actgaaacac acacacagtt
    11461 tggacaaggg atgagcctat cagaagcctt acaagctcga aaggcccagg ctcaagtaag
    11521 acaatcagca cagccaatgc aggcacatac gataccatca gcttcaacta gctcagtttt
    11581 accgtttggt tctaatagct ctgtagaaga ggtcacttgg cagattggcg atatcgctca
    11641 ccataaaaaa tggggagacg ggactgtgct tgaggtttca ggctccggaa aaacgatgga
    11701 attgaaaatc aaatttccag aagttggttt aaagaaatta ctagcaagtg ttgctcctat
    11761 tgagaaaaaa taagaaaaaa atgagcttcc tactatataa gtgagtagga agctcatttt
    11821 ttagatttct gtatcatata aaagttaaaa aattttaagt tttaggagca tatcagttgc
    11881 attttaaatt taaaggtgta taataacaat ataaattaaa taacgagtgg tcactttata
    11941 aaatattatc aaaataacat tttataggat tttgaaatat cggtattcaa acaaaaaata
    12001 aactaacact ttattattta gtttatatag tcactttttg aaagtcgact attaaaagac
    12061 tattgcttga agaataacag ctgttattct tccttggaca aaaggtacag gagtatctaa
    12121 aaaggagaat tatcatggga cttatttgga ctttaatcgt cggtgcgctt attggtgtga
    12181 ttgctggagc acttactaaa aaaggtggtt caatgggctg gattgcaaac atcgctgctg
    12241 gtctagttgg ttcttccgtt ggtcaagctt tacttggttc gtggggccct tctttagcag
    12301 gaatgtcttt gatcccgtca gttattggtg ccgttatcgt tgttatgatc acttcttttg
    12361 tactaaacaa aactaacaac tagtttcctc tttatttagt aagtacttac aaatagagaa
    12421 tgttaagtag aaaagaggtc tgtatgtcaa aattgttaaa gattagttat tgtcttgtcg
    12481 gacttgttct attgtctgtt tttggatggg tagttggaat aaccggaggc tatatatatt
    12541 taccttatag ttatcgttgg ctaagttggg gtatggatag ttttcccaat ctcttagatt
    12601 cagctttatc ctattattac ttctggacag cccttgtatt atttgttata acatttcttg
    12661 ccttattagt tattatttta tacccaagaa tttatactga ggtccagtta cgccataaaa
    12721 ataaaaaagg aacactttta ttaaaaaaat cggctattga aagttatgtt gcgaccgcta
    12781 ttcaaactgc aggtttgatg ccaaacccaa cagtcacagc taaattatat aagcggaagt
    12841 ttaagattat cgttaaagga cgtttagctt ctcgtgtagc agtggcagat caaattagtg
    12901 gcgtgaaaga agggatcgaa aaaggtttaa ctgagttctt cggaattaat tatcctgtta
    12961 atttcaaagt ttatgttaaa gatattgctg attctgatcg taaacatatt accagaaatc
    13021 gtgtagaata ggagatctat catggaattt tatgaaaaat tcaagtaccc tattattggt
    13081 ggcttggtgg ggctaattct tgccatattg ctcatggctt ttggtttatt caaaacgcta
    13141 ctagcaatta ttttcattat tttagggata tatggcggcc tgtatgccaa aaaaacaggt
    13201 attattgatc aatttcttaa tcgcaaataa aggagactta ttatgactga aacttatatt
    13261 aaaaacacat ctaaagactt aacttcagca attcgtggtc aattaacata tgatgataaa
    13321 gtaattgaaa aaattgttgg tttagcactt gaaaatgttg atgggttact gggtgtaaat
    13381 ggtggttttt ttgctaacct aaaagataaa ttagtaaaca ccgaatctgt acgtgatggc
    13441 gtgaacgtgg aagttggaaa aaaacaagtt gccgttgatc ttgatattgt cgctgaatat
    13501 caaaagcatg tgccgactat ttatgactct attaaatcaa tcgttgaaga agaagtgaag
    13561 agaatgaccg atctggatgt gattgaagtt aatgttaaag tagtcgacat taaaacaaaa
    13621 gaacaatttg aagctgaaaa agtaagttta caagacaaag tttcagacat ggcacgttca
    13681 acatctgaat ttacgagtca tcaagttgag aatgttaaag cttcagtgga taacggtgtt
    13741 gaaaaacttc aagatcaaaa agctgagcca cgtgtaaaat aattatatgt cgtcaattag
    13801 gtaagctaaa ggagggttta ttgtgtcaga tgaaaaatat aatgcgaaat tagatcaagc
    13861 tggtggcaag ttaaaagaag gatttggtaa gatttctgga gataaatctt tagaaactga
    13921 aggtaaagta gataaagtta ctggaaaagt taaagaggtt attgcagacg ccaaagatac
    13981 tgtaaaagga ttagctaaag gcttagataa taaagataaa taaaggggac atgatggaaa
    14041 aaactcaaat taaaagcact cttacttatg acgataaggt catcgaaaaa attgtcggtc
    14101 atgctttgga aaacgttggc ggattactag ctgtaacagg tggatttttc tcaaatatca
    14161 aaaataattt ggtgaattct gagtctgtaa ctgatggtgt ttcagttgaa gttggtagca
    14221 aggaagtagc agttgattta gcaatcattg tagaatacgg taaagatatt cctgctattg
    14281 cagaatctat taaagctatt gtttctcaaa acgttgacag tatgacacat ttgaaagttg
    14341 ttgaagtcaa tgtgaacgtt gttgatattc gtactaagga agaacatgaa gctgctagcg
    14401 ttactgttca agatcgtgtc actagtgcag cttcaagtac ttcacaattt gtatcagaac
    14461 aaacagaaaa attaaaagat actatctctg atactgtaaa tagtgatgag gcagctaaat
    14521 aagcaatcgt ctaggaagta ttattgacta gtggtgataa gtttgagctg gtatttacca
    14581 gctctttttg tcacggttaa aaagatacgg actattgtca tctctctaaa aatatgattt
    14641 aattagatag aaatttttca aattgttaga aaggagtaaa ttaagtgata agtcctaaaa
    14701 aagagataac aagttcaaaa tatcaaaaaa ttgctatttc agtagctcag cgtatcgcaa
    14761 atggcgaata tgaagtgggc gaaaagttaa agtcaagaac tactatcgcc tcgacattta
    14821 atgtatcacc agaaacggct cgtaaaggcc tgaatatttt agctgacttg aaaattttga
    14881 ctttaaaaca tggtagtgga gctattgtgc tgtcaaaaga aagagctatt gagtttataa
    14941 atcaatacga atcaacccat tccatcgctg ttttaaaaga aaaaatacgt gagactatta
    15001 atgatcaggg aaaagcaatg gaaaaaatgg ctgttttggt caatgatttt ttaatgcaaa
    15061 gtcaatctgt tagtaagcaa tacccattag caccttatga gattatttgt agtcaagatt
    15121 cggaacattt tggcaaatct attggtgtgt taaatatctg gcaccagaca ggagcaacta
    15181 tcgtcgctat tgaacatgct ggtcagttta tcgtttcacc aggtccttat tctgttattg
    15241 aaaaaggcga tcatatttat ttcgttggtg atgagtcagt catttcacga atgaagacct
    15301 tttttaattt acgaaaagga ttataagttt gaggacccac ctcaaacttt ttttgttgct
    15361 gtttagcatt attaaaagag gcagatcatc atggtttgtc tttccaaaat atctgtttag
    15421 acagttgttt tggattggga gcatgatttc agactctttt gatgtttttt aattgtctag
    15481 agtaactgct ttgagaataa tatcggcaac ctcttcagtg gtttcttgat agccattagc
    15541 aatccaatag ctgataatgc tctcaatact ggcaaggtaa acttctaaag catagggata
    15601 aggaatatgg tattgattgg tcacaatgtc ttggaaacca gtaatagtag tcaaaaattg
    15661 gtaacagaaa tccttgatga gttgcggaaa tttgaggtaa gaaatagtag cgagtgcggt
    15721 tataaattct ctatgttcta taaggtagga caaggtttga ttgaggactt gtcgagtgtc
    15781 tgtgtagatt tcagcctgat ttaacaaacg gtacaaatca tccagagtgt catttttgaa
    15841 gtgattcatc atgtcaaatt tatcagtgta gtggaggtag aaggttcctc tattgatacc
    15901 agccttttta gtaaggtcag agacagtaag agtttcaaaa gattgttcag ttagtaaggt
    15961 ggttaaggcg gtctttacat aagctttggt ttcagtatgt cttttggtca ttctcaggtg
    16021 ctccttaaat caacagttgc gtcagatttg ttcttgttaa atacttaggg ccattatata
    16081 ataaggctat aattaaatca acactatgtt caaataatgg aggggcagat ggcttttatt
    16141 gaattaaaac aggtttcaaa atcttatcag gtaggagaga cgactgtttt tgccaaccac
    16201 gaagtgtctt ttgagattaa taaaggagaa ctagtggtta ttttgggagc atccggagct
    16261 ggtaaatcga cagtcctcaa tattttgggg ggcatggaca cagtagatgc aggtcaagta
    16321 attattgatg gcaaagacat tgctcattac acatctaagg ccttaactca gtatcgtcgg
    16381 aatgctattg gtttcgtttt tcaattttat aatttggttc ctaatttgac agctaaagaa
    16441 aatgttgaat tagcagttga aattgtagca gatgctttag atcctgtgac cattttaaag
    16501 gaagtaggac tcagtcatcg tttggatcat tttcctgctc agctctcagg tggtgaacag
    16561 caacgggttt cgatagcacg tgccttagct aaaaacccta aattgcttct ttgtgatgaa
    16621 cctacaggtg cccttgacta ccaaacagga aagcaaattc taaccctctt acaggatatg
    16681 gcacgaacta aggggaccac ggtagttatt gtaacccaca atgctgctat tgctccgata
    16741 gctgatcgtg tcatttttat gcatgatgca caggttacca agacagtcat taataaggaa
    16801 cctgcaagca ttgagacaat tgattattaa gaggaaggtg tcgtatgata aagaaaacct
    16861 tatggaagga tattttgaga gctatcaaga atagtaaagg gcgattcatt tccttatttt
    16921 tcctaatggc cttaggctcc tttgctttag ttggacttaa agtcaccggt ccagatatgg
    16981 aacgaacagc cagccgttac ctcgaaagac accaggtgat ggacctaacg gtactggctt
    17041 ctcatcaatt ttcccaagcc gataaacaag agttagatac gttaaaaggg gctcatttag
    17101 aatatggtca tttacttgat gtcagtctaa ctagcaacca gaaatctctt aggctctata
    17161 gcgtgccaaa gaaagtgtct aagccagtct tggttaaagg gagttggcca aagcgagaga
    17221 cagatttggt tttatcctcc tcacttgcta aaaactatca gattggagat gaactagtag
    17281 tcacctcacc tatggaaggt ttgctgacga caacccattt tcaagtggtt ggttttgcga
    17341 attcttcaga agtttggtcc aagtccaatt taggaagttc ctcaaccgga gatggaagtc
    17401 tctacgctta tgcttttgtt aatccaaacg tttttaaaag tgcctttaac ctgctgcgca
    17461 tccgttttag taatcttcgt ctgacaaacg ctttttccaa agactatcaa aaaagggtaa
    17521 cccaaaacca agctcatttg gataatctac ttaaagataa tggccagaag cgttatgatg
    17581 acctccaaaa tcagtatgac cttgccttaa aaaatggcag agcagcactt gcaaaggaaa
    17641 cagtgaaact agctgcgagt gaggagaact taactttttt agaaggctct gctttacaag
    17701 aagctaagca tcagattgaa caaggcaaac aagcattagc caaggaggaa aagcagttag
    17761 agcaggtgca ggctacaaaa gataagctag aaaaacccag ttacctgact tataatcgct
    17821 cgaccctacc aggaggagaa ggatatcata cttatgcaac ttcaacgacc tccatttcaa
    17881 atgttggaaa tatttttcct gttgttcttt atctcgtagc tgctctagta gcctttacca
    17941 ccatgacacg ttatgttgat gaagaaagaa caagctctgg tctattaaaa gccatcggtt
    18001 attctaacaa ggatatcagt ttaaagtttc ttatctatgg gcttttagcc agttttttag
    18061 ggacaacttt aggtattatt gggggaactt acctcttatc tgccttgatt tcagagatct
    18121 taacaggagc tttgactatt ggaaagactc acctttatag ttattggttt tataatggca
    18181 tagcttactt gctggctatg ttatctgctg ttttaccagc ctacttaatt gtcaaaaagg
    18241 aattattcct caatgcagct cagttattgc tgcccaaacc tcctagtaag ggggcaaaaa
    18301 tctggttgga acacctgact tttgtctgga aagctctgtc ctttactcac aaggtgacca
    18361 tacgtaatat ttttcgctat aaacaaagaa tgctgatgac cattgtaggc gttgcaggct
    18421 ctgtagccct tttatttgca ggcttaggga ttcagtcgtc attagccaaa gtagttgagc
    18481 atcaatttgg tgatttaacg acttatgata ttttggctgt cggttcggcc aaagcgacag
    18541 cgacagagca aactgactta gctagctatc ttaaacaaga acctattaca gggtaccaaa
    18601 aggtatctta tgccagctta acccttcctg taaagggatt acctgataag caaagtattt
    18661 ccattttatc aagttcaact acctctctta gcccctattt taatctgctg gattgtcagg
    18721 agcaaaagaa ggttcccatt ccaacctctg gtgttttgat ttctgagaaa ttagcctctt
    18781 attacaaggt aaaaccaggt gatcagttgg tattgactga tcggaaagga cagtcttata
    18841 aagtgacgat taaacaggtt attgacatga cagttggcca ttacctgata atgtctaata
    18901 cctattttag gaatcatttt aaaggattgg aggctgctcc tgcctatctg attaaggtaa
    18961 aagacaagga tagcaagcac ataaaggaga cagccagtga cttgttaacc ttaaaagcga
    19021 ttagagcagt ttcacaaaac gtcaatcata ttaaatctgt tcagctagta gtcacctctc
    19081 ttaatcaggt catgaccctc cttgtcttct tgtctatttt attagcaatc gttatccttt
    19141 ataacttaac gactattaat attgctgagc gtattcgaga attatccact attaaagttc
    19201 tgggatttta cgatcaggag gtcaccttat atatttatcg agaaactatt tcgctatccc
    19261 tagtaggcat tcttttaggt atctatttag gaaaaggcct gcatacttat atcatgacaa
    19321 tgatttcaac tggggatatt caatttggtg taaaggttga tgcttatgtt tatctagtgc
    19381 caattctagt aatccttagc ttgttagcgg tattaggtat ctgggttaat cgccatttaa
    19441 aaaaggttga tatgttagaa gctttgaaat ccatagattg attggtgaaa agacagagat
    19501 agacagcact tgaaaagtta atcatattat gatagtatct aaatactata atttaataga
    19561 aagatactat gtatggaaaa tagcattgag gcctatctag cagatagcag agaaaaggta
    19621 acattaggta cagttgttga ttgttttaaa ggaaaagcag tctcaagtaa ggttgtgcca
    19681 ggtgatgtgg gattgattaa tttatctgac atgggcacct taggaattca atatcaccaa
    19741 ttaagaactt tccagatgga tcgtaggcag ctcttgcgtt accttttgga agacggagat
    19801 gtcttaatcg cctctaaggg aaccctaaaa aaagtctgtg tttttcataa gcaaaatcga
    19861 gatgtggtgg catcatcaaa tatcactgtc ttacgccctc aaaaactgtt aaggggctac
    19921 tatatcaagt tctttttgga cttacctatt ggtcaagcgc tcttggacgc agcggaccat
    19981 ggcaaagatg tgatcaatct ctcaacgaaa gaattgttgg atattccaat tcccgttatt
    20041 cctttagtga aacaggatta tttgattaat cactacttga gaggattaac agattatcat
    20101 cgtaaactca atcgagcaga acaggagtgg gaatatattc agaatgaaat tcaaaaagga
    20161 ttaggttagg agtcttgtca tgggttcagt tgaacgtaca ttaaaaatga ccttggctac
    20221 cattgttgcc attttaattg cttaccaact tcatctggat tatgcaatgt cagcggggat
    20281 cattgccctg ttaagtgttt tagacacccg taagtccagt ttggtggtcg ctaggaatcg
    20341 cttgctatct tttttcctgg cttttgggat tgcgatgatg tgctttagct tgtttggttt
    20401 tacgacagta ggttttatgt gttatttgtt aattattatt cctttgctct atcactttca
    20461 gatagaggca ggactggttc ctataacagt acttgtgaca catttgattg ctaaaaagag
    20521 tattgcatta cctattttat cgaatgagtt tatgctcttt tttgtaggga ctagtgtcgc
    20581 tttactattt aatgcttata tgggttcaca agatcagcaa attcggtact atcaccaaaa
    20641 agttgaatcg gatctgaaag gtatcttata ccgttttgaa agttttctac tggaaggaaa
    20701 aggtcaaaat gaagggctct tgatcaaaaa ccttgataaa atattagatg aagctctaaa
    20761 actagtttat cgagaaaggc ataatcaact atttcagcag accaattatc aagttcatta
    20821 ttttgagatg cgacgacaac aaaatagact attgggacaa atggctatca acgtaaacac
    20881 gttaatgaga caaagtaagg aaagtattct tttgtcacat ctttttcacg aaactgcttg
    20941 tcagctaagc gaacaaaatc cggctttaac cttgattgat gacatcgaac aattgcttga
    21001 aacctttcgt catggtgatc ttcctcaaac tcgggaggaa tttgagcgac gtgcggtttt
    21061 atttcagctc ttacaagact tagagcgctt tatcttatta aaggtagagt tttatcagga
    21121 ttatcaaaat gactaaatct ctattgcaca cttcttatct atgacagctt gggacttaac
    21181 ttcctgagct tttttgctat aataaaaaga agaatctcag aatggagaca agatgaacga
    21241 aataaaatgc cctcattgtc ataccctatt tactatcaat gagtcagagt atagtcaatt
    21301 attggaacaa gtcagagggc aagcctttga tgaagaattg caaaaacggc taatcaatga
    21361 gatagccttg ctagaagaaa aagcgaagca tcaattgcat gaggttgtcg caaaaaaaga
    21421 aacagctatt accagcttga caaaccaatt agagcaaatt gaaaaagaac aagcctacct
    21481 cagacaagaa gaactggcta aaaaagacca gcttattgct tctttagaag cgaaacttga
    21541 taaattagcc tcacaaaatg ctttggaact ggccaatcaa ttagctgaga aggacaaaga
    21601 agtggttagt ttgaccaatc agctggacaa attagcactt gaaaaagatg ccacgttcca
    21661 gtcaaagctt gctacaatcg aaaaagaacg cgatggaatc aaaaaccaat tggcgcttca
    21721 agctaaagaa agtgaattgt cgttggcttc ggtgcgcagc gactacgaag cacagttaaa
    21781 agcggctaac gagcaagttg aattttataa aaatttcaaa tcgcagcaat ctaccaaagc
    21841 aatcggagag agtctagaac tctatgctga aacagagttc aataaagtaa gaagttatgc
    21901 ttttcccaat gctagctttg ctaaagataa tcaattgtct agtcgtgggt caaaagggga
    21961 ttatatttac cgagaggttg atgccaatgg tgtagaaatc ctctctatca tgtttgaaat
    22021 gaaaaacgag gcagacacca ccaaaacaaa acataaaaat agtgattttt tcaaggagtt
    22081 agataaagac cgtcgcgaaa aaggttgtga gtatgctgtt ttggtaagca tgttagaagc
    22141 tgataatgat tactacaata cagggattgt tgatgtcagt catgaatatc aaaagatgta
    22201 tgtggttcgt cctcaactct ttatccagtt gatagggatt ctgcgaaatg cggccatgaa
    22261 tagtttacat tataagcaag agttggcctt gattaaggaa caaaatattg acatcactca
    22321 cttcgaagag gatttggacc aatttaagaa tgcctttgct aagaactatc agtctgccag
    22381 caacaacttt aaaaaggcta ttgacgagat tgataaatcg attaaacgca tggaggaagt
    22441 gaaacgcttt ttaaccacta gtgaaaatca gttacgctta gctaataata aattagagga
    22501 tgtttcagtt aaaaaactga cccgccaaaa cccaaccatg cgagagaaat ttgaagccct
    22561 caaggaccag taagcctgaa agtgagtctt tccctgctaa aattggttaa gcgtttattt
    22621 tgggaaaact gatgataaag cggtagaata gaatcagtaa taattactaa ttagagagaa
    22681 aaatagacat gataaatgga atcattaatt taaaaaaaga agccggcatg acctctcatg
    22741 acgcagtttt taaattacgg aagttgttac aagaaaaaaa gattggacat ggggggacct
    22801 tagatcctga tgtggtcggt gttctaccaa ttgcagttgg taaggctact cgtgtgatcg
    22861 aatacatgac agaagctggt aaagtttatg aaggacaagt gactctcggt tactcaacaa
    22921 cgactgagga tgctagtgga gaggtagttg cgaggtcctc tttgccagct gtgctaacag
    22981 aagaactcgt tgatcaaacg atgacgactt tccttgggaa aatcacacag accccaccta
    23041 tgtattcagc cgtaaaggtt aatggccgta aattatacga gtatgcgcgt gctggtgaaa
    23101 gtgtggaacg tcctagaaga gaagtgacta tttctctgtt tgaacgcacc agccctttga
    23161 attttactga agacggtctt tgccgattct cttttaaagt tgcttgtagc aaagggactt
    23221 atgtgcggac cttagcagtt gatttaggtc gagctctagg ggtagagagt catatgtcct
    23281 tcttgcagcg atcagcttca gcaggattaa ccctcgagac tgcctatact ttgggagaaa
    23341 ttgctgatat ggtttctaaa caagagatgt cttttttgtt accgatcgag tatggcgttg
    23401 ctgatcttcc taaaatggtt attgatgata ctgaattgac tgaaatttca tttggtcgcc
    23461 gtcttagtct ccctagtcaa gagccactgt tagctgcttt tcatggcgaa aaagtaattg
    23521 ctattttaga aaaaagagat caagaataca aacccaaaaa agttcttatt taaatagttt
    23581 gagtaagtag aatggataat tatggaaatt gaatatatca aagattatcg tgatattaac
    23641 caagaagatg atacagtctt ggtattggga tattttgatg gattacaccg tggtcataaa
    23701 gccttatttg acaaagcaag agaggttgca aataaagaag ggttaaaagt agtcgttttt
    23761 acgtttacag agtctccgaa attggctttt ttgcgttttt ctcctgagtt attgcttcat
    23821 attacttatc caaagaagcg ttatgaaaaa tttgccgatt acggtgtaaa taaattatat
    23881 ttggttgatt ttacttctaa attctctaag gtctcatctg atcattttat aactcattat
    23941 attaaaaacc tgaaagcaaa gcatattgtt gtcggttttg actataagtt tgggcataat
    24001 tgtacagata gtgattattt aaccagaaat tttgaaggac aagtttatac cattgaggaa
    24061 ataaaagaag atcatcgaaa aatttctgct acatggataa ggaagcttat tcaagaaggt
    24121 aatgtagtta aagcaaacca cttgttaggc tatgacttat cgacaagggg gagagtcgtt
    24181 cacggggatg cgcgtggccg tactattggt ttcccaacag ccaatctagc tccaattgac
    24241 aacacatact taccagctga tggagtctat gttacaaatg ttattgttgc taataaaatc
    24301 tatcgctcta tgacgagttt aggtaaaaat gtgacatttg gggggaaaga gttgcgtcta
    24361 gaagttaaca tttttgactt tgacgaagag atctatggag aaattattga gattgtttgg
    24421 ttagataaga ttcgagacat ggaaaaattc gagggaatcg aagatttgac tgatagattg
    24481 gaatatgata aacgaacagc attaaattgg aaaaaagata gcaaattatc ttaaattttt
    24541 gtataataag gtaagtaaat ttgatagagg aaaaatatgg ttaccttatt tttatcacct
    24601 agctgtacca gttgtcgtaa agcaagggca tggttagtaa aacacgaagt tgactttcaa
    24661 gagcataata ttattacaag tccattaagc cgtgatgaat taatgtcaat cctctctttt
    24721 acagagaatg gaacggagga tattatttca acacgttcaa aggtttttca aaaacttgat
    24781 gttgatgtag aagagctttc aatttcagat ttaattgatt taattgctaa aaatccaagt
    24841 cttcttcgtc gccctattat catggatcaa aaacgcatgc aaattggttt taatgaagat
    24901 gaaattagag cgtttttatc gagagattat cgcaagcaag aactacgtca agcaacgata
    24961 aaagcagaaa ttgaggggtg atatgtcagg taattattat tatccacttg acttaagttg
    25021 gagcactgaa gaaatatcct cagtgcttca ttttttgaat aaagtggagt tagcatatga
    25081 aaaaaaagta gatgcaaaac aactcctaaa tagttataag acttataaaa ccattgtcaa
    25141 aagtaaagct caggaaaagc aaatcgaccg tgattttcag aaagttagtg gttattcgac
    25201 ctatcaagtt gttaaaaaag cgaaggcaat tgagaaagga ttcttttccc ttggaaacta
    25261 aatacgcatt tgcaaggcaa atcattaaag aagcagggct ttttatcaag tcaaaaatgt
    25321 cagagcagct agatattcag gtgaaaacac aatttgatga tctggttacc aatgttgatc
    25381 aagaaactca acagttactg atggatcgta ttcatcagac ctatccttgt gatgccattt
    25441 tagctgaaga aaatgacgtt cgtcacccca ttaatcaagg aaatgtttgg gtcatcgatc
    25501 ccattgatgg aacggttaac tttattgtgc agggatcaca gtttgcagtg atgattgcct
    25561 attatgaaca aggaattggc caatttgggc ttatttatga tgtgatggca gatcaattac
    25621 tagctggtgg tggtgatttt gaagttactt taaacggtga taagcttcca gcttaccaag
    25681 aaaaaccttt agagcgtagc ttaattggct gcaatgcagg catgtttgcc agaaatgatc
    25741 gcaaccttgc tcatctaatt gcaaagactt taggtgtaag ggtttatggt ggtgcaggta
    25801 tctgcatggt taaggtcatg aagcaggaat tactggccta tttttcattt atccaaccat
    25861 gggattacgc agcagcaaaa gtactaggcg ataaactggg ttatgtttta ctaactattg
    25921 acggatatga gcctgatttt caaacacgtc aaaaaatcat gtttgttcct aaatgccaac
    25981 tcactaggat agcgtctttt ctaacaaagg actctgccgt cacacttgaa gatcaataga
    26041 aatgatgtca ttaccaaaag aatttattaa cacttatcaa gcaatactag gcaaagaatt
    26101 agaagatttc ttggcaagtt ttaaccaaga gccggtcaat gcttttcgga ttaacccctt
    26161 aaagaatcaa ctaaaaacct ttgaacaccc cattccaaat accttatggg gatattatgg
    26221 taaactatct ggcaagtctc cagagcatgt gtcggggttg gtttattccc aagaaccagc
    26281 ggctcaaatg gtggcacaag tagcggctcc tcaaaagggg agtcgtgttt tagacttggc
    26341 agctgcacca ggaggaaaat caactcattt attagcttat ttagacaata caggtctttt
    26401 agtcagcaat gaaatttcta agaagcgcag caaggtttta gttgaaaata tcgaacgttt
    26461 tggcgctaga aatgttgtgg tgactaatga atccgcagat cgtttagcca aggtattttc
    26521 tcattatttt gatactatcg tctttgatgg accatgttct ggtgaaggaa tgtttcgcaa
    26581 ggaccctgat gctattcaat attggcatca cgggtatccc gcagaatgtg ccaagttgca
    26641 aaaatctatt ttagaagacg ccttagccat gttaaaacca ggaggagaat tgatatactc
    26701 cacctgtact tgggctcctg aagaaaatga agatgttgtg cagtggctct tagagactta
    26761 taccttttta gaattggtcg atgttccaaa actaaatgga atggttagtg gtatcggact
    26821 tccagagact gctagaatgt accctcatcg ttatcaaggg gaagggcaat ttgtggccaa
    26881 gctaaaagat aagcgtcaag aaggacaaag cactaaacta aaagcaccta aatccaactt
    26941 gatcaaagac cagttacgtc tttggaaaat gtttgaaaaa gaccatataa accttaccct
    27001 atcaggaaca ctacaaacct ttggtgatta cctatacctc ttgccagatg gtcttcctgc
    27061 tttagatggc cttaaaattg ctcggaacgg tttagaatta ggcgtcttca agaagaaacg
    27121 ctttgaaccc tcttacgcct tgggattagc tttaaagcct gatgaggtcc gatcatctat
    27181 tgagcttaga gaggaagact ttagacctta tgtgtcaggt aacgtcatta ctttgcccca
    27241 atcttatcca acaggctggt accaattact cattaatggc aatggtctag gatttgccaa
    27301 ggtcgttgac aagaccatca aaaataattt ccctaaaggg ttaagatttt agcgtaaaaa
    27361 ataagattaa aaagttaaat atttaaagaa aaaatgcttt caaagcatag gtagatatgt
    27421 tactataaag aatatgtggc acttagtttc tacatatata ccctttattt ttacgaatag
    27481 gaaataaata atgaaaatga aaaaaaaatt ctttttgtta agtcttttgg tcctatcaac
    27541 tttcttttta tccgcatgtt ctagctggat tgataaaggt gagtcaataa ccgctgtagg
    27601 atcaacagca ctacaaccct tagtagaagc agtagctgat gaatttggaa gcagtaatct
    27661 aggcaagact gtcaatgttc aaggtggtgg ttcaggtaca gggttgtctc aagttcaatc
    27721 aggagctgtc caaattggaa atagtgatgt ctttgcggaa gaaaaagatg gtattgatgc
    27781 ttctaaatta gttgatcatc aagtagctgt tgcaggactt gcagttattg ccaatcctaa
    27841 agtcaaggtt tccaatctca gtagtcagca gttgcaaaag attttttcag gagaatatac
    27901 caattggaaa caagttggag gagaagatct tgcgatttca gtgatcaacc gagcagcaag
    27961 ttctggctca cgagcaacct ttgacagtgt tatcatgaaa ggggtcaacg ctaaacaaag
    28021 tcaagagcaa gactccaatg ggatggttaa atcgattgtt tcacaaacac caggtgccat
    28081 ttcttacctt tcctttgcct acgttgattc atctgttaaa tctttgcaat taaatgggtt
    28141 taaggcaaat gctaagaatg tggctacaaa tgattggcca atctggtcct acgaacacat
    28201 gtacaccaaa gataaaccaa cagggttgac caaggaattt cttgattata tgttttcaga
    28261 tgaagtacaa cagaacattg ttacacatat gggatatatt tcgataaatg atatggaagt
    28321 ggtcaaatct catgatggaa aagtaacaaa aaggtaatgg aggaattatg gaaaatcagg
    28381 aattggctaa aaaattagcc tcaccatcaa agaattcaag acttgaaacc tttggccgca
    28441 ccattacgtt tttgtgtctg gctttgattg tttttatcgt tgcgatgatt ctgatcttcg
    28501 ttgctcaaaa agggttgtca accttcttta ttgacaaggt taatcttttt gatttcttgt
    28561 ttggaaagga gtggcaacca agtgtgaaaa atgcggccgg cattccttat cttggtgctc
    28621 tgccaatgat cacaggatcc tttttggtta ccgttttatc tgctattatt gccaccccat
    28681 ttgccattgg cgcagccgtt tttatgactg aaatctcacc taaatatggc gctaaattat
    28741 tacagcctgc ggttgagctt ttggttggaa ttccttcggt tgtttatggg tttatcggtt
    28801 tgcaagtgat tgttcctttt atgcgctcta tctttggtgg cacaggtttt ggaatcctat
    28861 ctggggtctg tgtcttgttt gttatgattt taccaacagt gacttttatg acaacagaca
    28921 gtctgcgggc ggtgcctcgt cattaccgcg aagcgtctat ggctatggga gcaacacgtt
    28981 ggcaaaccat ttggcgtgtt gtccttaatg cagctcgtcc aggaattttt accgctgtta
    29041 tttttggaat ggcgagagct tttggagaag ccttggctat ccaaatggta gtcggtaact
    29101 ctgctgtaat gccaagttca ctaacgacac cagccgcaac cttaacgtct gtcttgacaa
    29161 tgggtattgg taacacggtt atgggaacgg tccaaaataa tgtgctttgg tcccttgccc
    29221 tagtcttatt attaatgagt ctggccttca attcccttgt caaattaatc acgaaagaaa
    29281 gaaagagaaa ttatgaacgc taaaaaagtc gataaagtag caactggtac tctttatacc
    29341 attgctggaa ttattgtagc tattttagct tccttaattc tatatatcct tgtccgtggc
    29401 ttgccacaca tcagctggtc ctttttaaca ggaaaatcgt cttcgtacga agctggtggg
    29461 ggaattggta tccagttgta taattccttc ttcctattga ttgttaccct gattatttcc
    29521 attcctttat caactggagc ggggatttac ttggctgaat atgccaaaaa agggcctgtt
    29581 accaacttta ttagaacctg tattgagatt ctgtcttccc taccatctgt ggttgtaggg
    29641 ctctttggtt acttgatttt cgttgtgcag tttgaatatg gcttttctat tatttcaggg
    29701 gctcttgctt tgacggtctt taatcttcct caaatgaccc gtaatgttga agatagttta
    29761 ctgcatgttc atcataccca aagagaagca ggattagctt taggcctgtc acgctgggaa
    29821 acagtttttt atgtggttat cccagaagct ctcccgggaa tggtaacagg tattgtctta
    29881 gcttcaggtc gtatttttgg tgaagcggca gcgcttatct atactgctgg tcaatcagca
    29941 ccagctctgg attggtcaaa ttggaatcca cttagtgtta ctagtccaat ctctattttc
    30001 cgtcaatctg agacccttgc tgttcatatt tggaaagtca atagcgaagg gacaattcca
    30061 gatgctactc ttgtatccgc aggaagtgca gcagtgctat taatttttat ccttattttc
    30121 aacttttcag ctcgctttat tgggaagaaa cttcattcta aaatgacagc agcgaaataa
    30181 gatattggag aaatcatgac agaatataat tggaatgaac gtcatatcat cacttttcct
    30241 gaagaaacgc ttgctctagc taccaaggat ttacatgttt actatggtgc gaaagaagct
    30301 atcaaaggga ttgatatgca gtttgaaaaa cataaaataa cagccttgat tggtccatct
    30361 ggttgtggca aatcaactta tcttcgtagt cttaaccgca tgaacgatac cattgatatt
    30421 gctcgggtta caggtgagat tctttatcag ggcattgatg ttaatcgaaa agacatgaat
    30481 gtttacgaga ttcgcaagca cttaggaatg gtttttcaaa gacccaatcc tttcgctaaa
    30541 tccatttata aaaacattac ctttgcccat gaaagagctg gtgtgaaaga caaaaaagtt
    30601 ttagatgaaa ttgttgagac ttcactgaaa caagccgcgc tttgggatca agttaaagat
    30661 gaccttcaca aatcagcctt caccttatca gggggtcagc aacaacgctt gtgtattgct
    30721 agagccattt ctgtcaaacc tgatattcta ttaatggatg aaccagcctc tgcccttgac
    30781 ccgattgcaa cgatgcagct ggaagaaacc atgtttgaat tgaagaagaa ttacacgatt
    30841 attattgtga cccacaatat gcaacaagca gctcgtgcca gtgattacac tgccttcttc
    30901 tatttaggca acctcattga atatgataag acacgtaata ttttccaaaa tgcgcaatgt
    30961 cagtcaacaa acgactacgt ttcagggcac tttggttaga aaggcaatat gatgacagaa
    31021 cctattttac agatcagaga cctttctgtt tactacaatc aaaagaaaac gttaaaagat
    31081 gtctctttgg acctttatcc aaatgaaata acagctttaa ttggtccttc tggatctggg
    31141 aaatcaaccc tcttgcgttc tatcaaccgt atgaatgatt tgaatcctga agtaaccatt
    31201 acaggctcta tcgtctataa cggacacaat atttatagtc caaggacaga tacggttgat
    31261 ctacgcaaag aaattggtat ggtattccaa caacccaacc catttccgat gtctatttat
    31321 gagaacgtgg tttacggcct tcgcttaaaa ggtatcagag ataaatcaat cttagaccat
    31381 gctgttgaaa gctctcttaa aggggcttcc atttggaatg aagtcaaaga tcgtttacat
    31441 gattctgcgg ttggcttatc aggcggacag caacagagag tttgcatagc tagagttctt
    31501 gctaccagcc ccagaataat ccttttagat gaacctacct cagctcttga tcctatctca
    31561 gctgggaaaa ttgaagaaac attattgtta ttaaaaaaag attatacact ggctattgtg
    31621 acccgctcaa tgcagcaagc ctcacgttta tcagatcgaa caggtttttt cttggagggt
    31681 gacttacttg agtgtggtcc tacaaaagct atgtttatga atcctaagcg caaagaaacc
    31741 gaagactata tttctggtaa atttggttga gaaagacgta tatggttgac ccctctgtgt
    31801 ttttattata atgactagat tgtaggaaga aaaaatcatg ttaagaacga aatttgaaga
    31861 agaattagat aaattgcata accagtttta ttcaatggga acggaagtat tggcacaaat
    31921 caataaaact gttcgtgcct ttgttagtca tgatcgtgaa ttggccaaag aagttatcga
    31981 agaagatgac actattaata attttgagac aaagcttgaa aagaaatcgc tggaaattat
    32041 tgccctacaa cagccagttt cgaatgatct tagaatggtt attactgttt taaaggcatc
    32101 aagtgatatt gaacgtatgg gtgaccatgc tgcttcgatt gccaaggcca ctattcgtat
    32161 gaaaggtgag gagcgtattc cagttgttga agaacaaatc aaccttatgg gaaaagctgt
    32221 gaaacagatg gtagaagaag cccttaatgc atatatcaat gctgacgata caaaagctta
    32281 tgaaatcgca gcttctgatg agattatcga tcaatatttc cgtaatatcc aaaccttagc
    32341 tgttgaagaa atccgtaagt ctccagatgc tgtttttgca ggaaaagaat attttcaagt
    32401 attaatgtat ttggaacgta ttggtgacta tgctcgcaac atttgtgaat ggattgttta
    32461 tcttaaaact ggaaaaatta ttgaattata agaaattatg ctataatgag actgggaaac
    32521 agcttgccag tccctggttt tatacgaaat tgtgcaagtt agaaaatgaa ctaacaagaa
    32581 aagttttctt tgctgaatct gattttgtta ggttaacaaa tatatgtaga cagagatgat
    32641 acttaggtat catctgttga tctattatga tttgttatta gaattaagga gttatatgaa
    32701 gacagtagaa catctcatcg aaacatttgt gcctgaaaat tataacatct ttttagatat
    32761 taatcgtcag actaaaacat ttacggggaa tgttgcgatt aatggtgaag ctttagataa
    32821 tcatgtggct ttccaccaaa aagatttgga cattaagtct attttgctcg ataatgaagc
    32881 tgttatttat caagtggata acgacaatga agttgtgcgt gttgaattac ctgaaacagg
    32941 aatgatgacg cttgtcattg aattttcagg tagcattaca gataatatga caggtattta
    33001 cccttcatat tacactaaaa atggtgagaa aaaagaagtc atttcaactc agtttgaaag
    33061 ccattttgca cgtgaagctt tcccatgtat cgatgaacca caagcaaaag ctacttttga
    33121 tttaagcttg acctttgatc aagaaattgg tgagatagcg ttatcgaata tgccagaagt
    33181 caatattgac cgtcgcgagg agactggtct ttggacattt gatacgacac caagaatgtc
    33241 atcttacttg ctagcgtttg cgctaggaga acttcatgga aagactgtag aaagcaaaaa
    33301 aggaacaact gttggtgtgt atgcgacaac agcgcatcct cttagctcgc ttgatttttc
    33361 attggatatt gcagttcgtg ttatcaactt ttatgaagac tattttggag tacattaccc
    33421 aatcccgcaa tctcttaata ttgcgcttcc agatttttca tctggtgcta tggaaaattg
    33481 gggtttaatc acttatcgtg aaatttattt attagtagat gaaaattcga ctgttcaaag
    33541 ccgacaacaa gttgcacttg taatcgccca cgagatagca caccaatggt ttggaaactt
    33601 ggttacaatg aaatggtggg acgatctgtg gttaaatgag agttttgcca acatgatgga
    33661 atatgtctct atcgaggcta ttgagccgtc ttggaaaata atagaagatt tccaaacagg
    33721 cggtattcct cttgctttaa aacgtgatgc tactgatggt gttcaatcag tgcatgttga
    33781 agtcaatcat ccagatgaga taaatacatt gtttgatcct gctattgtct atgcaaaagg
    33841 tagtcgtttg atgcatatgt tacgccgttt tattggtgat cgtgattttg ccattggctt
    33901 acatcactac tttgaaaaat atcaatatcg aaatactgtc gggcgtgatt tatggaatat
    33961 tttatcagat acttcaggca aagacgtagc tgcttttatg gatgcttggc ttgaacaacc
    34021 aggttatcca gttttaacag ctcgccttga aaacgatcaa ttgattttga gtcaaaaaca
    34081 attttttatt ggaaaaggtg aagaaaaagg tcgcctttgg ccgattcctt taaacactaa
    34141 ttggcatggc cttcctgaaa ctttgacaga ggcagaaatg gtcattccaa actttagtca
    34201 attggcagca gaaaatgaag gagctctacg tttcaatatt gataatacag ctcattacat
    34261 tacagattac caaggatcat tattagatgc cttagtcact gaacttgccc aacttgataa
    34321 tacttctgct ttacaagtta ttcaagaacg tcgtttattg gcagatagtg gcttgatttc
    34381 ctatgctgaa ttggttgact taattgctca attggacgat tctaagtctt atatggtagc
    34441 agaagcggtt cagcaagtgg tctctggttt gaaacgcttc attgatgaag gaagcttagc
    34501 agaaaaatcc tttaatcgct tggtaaccac catctatcaa gaagacttta atcagcatgg
    34561 ttttgagaaa aaagctgacg aatcagatga agatgagatg gtacgtcagg ttgccttggg
    34621 acgcttgtgg ctagcagaaa atcagacgat tattgacgga ttgagaacta tttttgaagc
    34681 atatcaaaac aatattgcaa gtattccagc agctgttcgt cgcttggttt tagccaacca
    34741 aatgaaatat tttgaaacag atagcttggt tgatatttat tttgagactt atgtagcaac
    34801 tactgataat aatttacgca gtgatttaac ggttgctttt agtcaaacaa aacaatcaac
    34861 tactattaga cgtattcttg ttagtttaaa agataaagat attattaaac ctcaggattt
    34921 atcttattgg tacaatgctt tgcttggaca aagctttact caagatatca tttgggaatg
    34981 ggctagagaa aattgggatt ggattaaatc tgcacttggt ggagatatga gctttgataa
    35041 atttgttatt tatccagcaa gtaactttaa aacaccaaaa catctagcag aatataaatc
    35101 tttctttgaa cctaagctag atgatatggc tattagccgt aatattacca tgggaattaa
    35161 tgaaattgaa gcacgtgttg ctttgatcac taaagaaaaa gaagcagtta ttgcagctct
    35221 cagtcattac tagaccataa gcaaaagagt tggggggact ttccagctct tttttgtagt
    35281 gagctagctt cctttcttag ttccgtctat aactaaatga ttatccatag tttaattcaa
    35341 aaggagattg cactttaaag cgaaggctat tagcctctta aatacttttt taccaggtgg
    35401 tgaataagaa caattataaa tgatttaatc attatgcttt aaatgcctta aaggagtatt
    35461 aatattttag taaaccttaa gctttttgta gtatagtctc gttagaaatt gttatcagaa
    35521 atgagactaa gttaaatgaa gtgatagtta ctttatttaa gaacaattga ggtagagaca
    35581 tgataaaaat attattagta gaagatgact tgagcttatc aaattccatt tttgattttt
    35641 tagatgattt tgcagacgtc atgcaagttt ttgatggcga tgaaggttta tacgaagcag
    35701 aaagtggtat ttatgatttg attttgttag accttatgtt gccggagaaa aatggctttc
    35761 aagtcttaaa agaattgcga gaaaaagata ttaaaattcc tgtgttgatc atgacagcta
    35821 aagagggttt agatgataaa ggtcacggat ttgaattagg tgctgatgat tatctgacaa
    35881 agccgttcta tttagaagag ttaaaaatgc gaattcaagc actgttaaaa agaacaggga
    35941 aatttgcaga taaaaatatt agttttggca atttagtggt cgacttagct cgaaaagagg
    36001 tgaaggttga agggaaagta gttgaattac ttggcaaaga atttgatttg ttggtgtatc
    36061 ttttgcaaaa tcaaaacgtt attttaccaa agacacaaat ttttgatcgt ttatggggat
    36121 tcgatagtga tactacgatt tcggttgttg aagtttacat ttctaaaata aggaaaaaat
    36181 taaaaggtac ccgttttgtt aacaggcttc aaactttaag aagcgtagga tatattctaa
    36241 aaaacaatga ataaattaaa aaaagagatt ttatcagata actataacca cttttttcat
    36301 ttttttgcgg tttttacagg tatttttgtc attatgacta ttattatctt acagattatg
    36361 cggtttggcg tttattcgtc agttgacagt agtttagttt ctgttagtaa taatgcaagt
    36421 agctatgcta atcgtacgat ggctagaata tcttcttttt actttgatac tgaaaataac
    36481 attattaagg cgctgcctga ttcagatagt tctaagttat taggaacgcc tgcagctaat
    36541 acagatatca ttttgtttag tgctaatgga acaattttaa atgcttttga tgcgttttct
    36601 aactatcaaa attttcattt agataaacgc cggttgggga gtattgaaac caccagttta
    36661 atgaattttt atggacaaga agaaaaatac catacgataa ctgtaagggt tcatatcaaa
    36721 aattatcctg cagttgccta tatgatggca gtagtaaatg tggaacaatt agaccgcgct
    36781 aatgaacgtt atgagcgcat tattattata gttatgagtg ttttttggct aatttctatt
    36841 ttagcaagta tttatttagc caagtggagc agaaaaccta ttttagaaag ctatgaaaaa
    36901 caaaaaatgt ttgttgaaaa tgctagtcat gaactaagga cccctttggc ggtcttacag
    36961 aatcgtctgg aatcgctttt tcgtaagccc aacgaaacga tattagaaaa tagtgagcat
    37021 ctcgcttcta gtttagacga ggttcgcaac atgcgcatct taacaactaa tttattaaat
    37081 ttagcaagac gagatgatgg cattaatcca cagtggactc atttagatac agattttttt
    37141 aatgctattt ttgagaatta tgaactagtt gctaaagaat atggaaaaat attttatttt
    37201 cagaaccaag tcaatagatc gttaagaatg gataaggctt tactaaaaca attaataacg
    37261 attttatttg acaatgctat taaatataca gataaaaatg gtattattga aattatagtg
    37321 aaaacaacgg acaaaaattt attaatttct gttattgata atggtccagg gataacagat
    37381 gaagaaaaga aaaagatttt tgatcgtttt tatcgagttg acaaagctag aacacggcaa
    37441 acaggtggat ttggcttggg gttggcttta gctcagcaaa tcgtgatgtc tttaaaagga
    37501 aatattacag taaaggataa tgatcctaaa ggtagtattt ttgaagtcaa actataagct
    37561 aaaaagggcg accaataagg tcagcccttt taaagttatt tttaactgtc aacgctttat
    37621 tgtatttttt gagtttttac cgtataaaaa aactccagtt ggagttttca ttggatattt
    37681 attagccaag ttttgcagca aggcgtgctt tatcgcggct tgctttgttt ttgtgaatca
    37741 aaccttttga ttcagcttta tcgatgcttg aagaagcagc gcggaaaagc tcttcagatg
    37801 ggttagcttc aaatgctttg atagcagtac gcatagctga tttttgtgct gagttttttt
    37861 cgtttgcttt aacgttaagt tcagcgcgtt taatagctga tttaatattt gccaatgttt
    37921 tcacctccaa attaatctaa ctacattatt atatagaaaa tatatgcgtt tggcaaccct
    37981 ttatcacttt ttgaggtaaa tttcatcaat tttatgatca gcacttttat gtaaaattaa
    38041 ttcagcacgg ttacgtgtag gttcaatgaa tttttcaaga ttttctaaat taacggtttt
    38101 ccaaacgttg cgcgcaaatg ctatagcctc agatcgaggt aattgtgcat attgggcata
    38161 ataattatga gggtcacgtt ttgctaattt taaaatgctt agaaaccgtt caatgtacca
    38221 ggtttcgatg tgactgctgt ctgcatcaat ataaatagaa aaatcaaagt aatcactcat
    38281 atataaacga ttgttttgtt gattttgaaa aacattaata ccttcgacga taaggaagtc
    38341 agggttatta aatgattgtt ttttatttgg gataatatcg taaatatcat gagaatagac
    38401 gggtgcaaaa gcagtttgac catttttgat ggaatctaga aaatctaaga gaagttccat
    38461 gttataagat tctggaaatc ctttacgatt taacagtcct tgttcaataa gaaactgatt
    38521 aggatataaa aaaccatcag tcgttaccag ttcgacttga ctattggggt gcgttcgcga
    38581 taataaaagt tgtaaaagac gactagttgt tgatttacca acagctactg aaccagaaat
    38641 tccaataata aaaggcctta attgaatgtc tttttttaga aaaaggcttt tagaaaaaga
    38701 aagattttct tgcgcaattt tgtaaacctg gatcagatta attaatggaa ggtagatatc
    38761 aatgacatcg ttaatactga tgttatcatt taagctagta atacttttaa gttcctcttg
    38821 tgtgagaaga gcttttgctt tttgatgtaa tgttttccaa gactctctag aaattttttc
    38881 aaagttgata aattcatttg acatggtagc tcctaatcat ttgacagtgc ttacattata
    38941 ccattttagt taaaaaataa ataatttttc gttagtattt acacgaaaat taatacaatt
    39001 aactcaaaaa agccacacat tcctagtaca attatcctgc tatattctaa caaatagtta
    39061 aagttcggtt tttatttgta atatagagaa aattgttaag aaacaagcgt cttgtaaacc
    39121 atttcaaagt atgatagaat ggaaaagtac cttaaaaaga taggaaaaat gatgacaaag
    39181 atgtattatg atgaaaatcc agatagccta cacgatattc atgaagtaaa agtagagctg
    39241 cttaatcatc cctttacttt tttgacggat tcaggtgtct tttcaaagaa aatggttgat
    39301 ttcggtagcc aagtattatt agagacacta aatttcaagg agaacgaacg tgtactggac
    39361 ttaggttgcg gctatggtcc tcttgggatt agtctagcca aggttcaaag ggtagacgca
    39421 accttagtgg acatcaataa tcgtgctctt gatttagcta gaaaaaatgc aactaataac
    39481 caagtggcag taactatttt tcaatcaaat atttatgaaa atattagtgg ccattttgag
    39541 catattatta gtaatcctcc tattagagca ggaaagagag tagttcatag tattattgaa
    39601 aaaagcatag attttttagt ggttaatggt gacttaacga ttgtgattca aaaaaaacaa
    39661 ggagctccta gcgctaaagc aaaaatggca actatttttg gtaatgttga gatattaagg
    39721 aaagataaag gttattatgt attaagaagt ataaagcaag cctaatttaa ataatcaaca
    39781 attgctcatt ttaattaggt ttgcttaaat agtaaagagt tatttttaaa ttatagataa
    39841 ctaatattat actagggtag cccctctact cgagtaacct tttttaatgt attttaactg
    39901 aaaaaggctt tcataatttt aaaaaaatgt taaaataggt gtataacaac tgattaccaa
    39961 gagattatta tcattatctt ctaaggtgtc attgttatgg tgacaatcaa ccatacgcta
    40021 ctaaagatag gtagcgttcg atcacaggtt gatcaagtga ttgaaaaatc aatatttata
    40081 attttacaaa tatctgtatc aatccatgtt gaatagtcta tgcttctaag gtgtcgacgt
    40141 taatgtactg acgggaccgt tatagtcttt ctttttggag aaacgtcgga tgccagtgaa
    40201 ctagttgaag ttaaagatgt taattcaaac agtgctgaag agattgatat cgttttgaac
    40261 ggtcgtttac ttcaaagcaa agaaattacc agtctatcgg agatgatata agtgttaaag
    40321 ttagatcacg ttaattatct atcaagtcgg gtgcatattt cgttaaagta tccacagggg
    40381 tttcaataga tgtagctatt atgaaaggtg atgttcaatg gggactactg atttagttag
    40441 ttgtgctgta caagctagtg agtatgctta tgtcccttac tctcattttc cagtaggagc
    40501 agctttaaaa acaaaagatg gaacgatcta tactggctgt aatattgaaa atgttagttt
    40561 tggtctgaca aattgtggag agcgaacagc tatatttaaa gccatatctg atggtcataa
    40621 agaattggtt gaaattgcta tttatggaga aactatgcaa cctgtatctc cttgtggggc
    40681 ttgtcgtcag gtaatggcag aattttttgc tccttcttct ttagtcactt taattgctaa
    40741 aaatggtcaa actgttgaaa tgactgttgg agatttactc ccctattctt ttacagattt
//
LOCUS       DAUCLR010000005        40263 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.005, whole genome shotgun sequence.
ACCESSION   DAUCLR010000005 DAUCLR010000000
VERSION     DAUCLR010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40263
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.005"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            160..1392
                     /locus_tag="VGT87_000189"
     CDS             160..1392
                     /locus_tag="VGT87_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEQ3813081.1"
                     /translation="MIKRLISLVVIALFFAASTVSGEEYSVTAKHAIAVDLESGKVLY
                     EKDAKEVVPVASVSKLLTTYLVYKEVSKGKLNWDSPVTISNYPYELTTNYTISNVPLD
                     KRKYTVKELLSALVVNNANSPAIALAEKIGGTEPKFVDKMKKQLRQWGISDAKVVNST
                     GLTNHFLGANTYPNTEPDDENCFCATDLAIIARHLLLEFPEVLKLSSKSSTIFDGQTI
                     YSYNYMLKGMPCYREGVDGLFVGYSKKAGASFVATSVENQMRVITVVLNADQSHEDDL
                     AIFKTTNQLLQYLLINFQKVQLIENNKPVKTLSVLDSPEKTVKLVAQNSLFFIKPIHT
                     KTKNTVHITKKSSTMIAPLSKGQVLGRATLQDKHLIGQGYLDTPPSINLILQKNISKS
                     FFLKVWWNRFVRYVNTSL"
     gene            1560..2741
                     /locus_tag="VGT87_000190"
     CDS             1560..2741
                     /locus_tag="VGT87_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEQ3813082.1"
                     /translation="MTKRIILTIFTFICFSMMPLVHAEDVMDITRQAGYTVSEVNRPK
                     SSIVVDANSSDILWQDNIDIPRDPASMSKMFTLYILFEELAKGKITMDTTITATPTDQ
                     AIANIYEISNNNIVAGVAYPIRDLITMTAVPSSNAATVMIANYLSNNDASAFIDRINA
                     TAKQLGMTNTHFSNASGAAAQAFQGYYNPTKYDLSASNITTARDLSKLLYAFLKKYPE
                     IISFTNKSVVHTMVGTPYEEEFHTYNHSLPDNQFGIKGVDGLKTGSSPSAAFNAMITA
                     KRGKTRLITIVMGVGDWSDQNGEFYRHPFVNALTEKGFKDSKTLSKKARQKLEKLVPQ
                     TKKETSPKQQHFKATKKQSYLERVEDFMNHNHTFLLICLAIFIITILLLSLVVFAMGR
                     Q"
     gene            complement(2893..4311)
                     /gene="sufB"
                     /locus_tag="VGT87_000191"
     CDS             complement(2893..4311)
                     /gene="sufB"
                     /locus_tag="VGT87_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEQ3813083.1"
                     /translation="MSDINEKVEPKPIDLGDYQFGFHDDVEPIYSTGKGLSEAVVREL
                     SAAKNEPEWMLEFRLKSLETFNKMPMQTWGADLSDINFDDIIYYQKASDKPARSWDDV
                     PEKIKETFDRIGIPEAERAYLAGASAQYESEVVYHNMKGEFEKLGIIFTDTDSALKEY
                     PDLFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENTGQ
                     FERTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVY
                     NLVTKRARALTDATVEWIDGNLGAKTTMKYPSVYLDGPGARGTMLSIAFANAGQHQDT
                     GAKMIHNAPHTSSSIVSKSIAKSGGKVDYRGQVTFNKQSKKSVSHIECDTILMDDISK
                     SDTIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKE
                     LPMEYAVELNRLISYEMEGSVG"
     gene            complement(4304..4783)
                     /locus_tag="VGT87_000192"
     CDS             complement(4304..4783)
                     /locus_tag="VGT87_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEQ3813084.1"
                     /translation="MALSKLNHLYMAVVADHSKRPHHHGQLDGVEAVQLNNPTCGDVI
                     SLTVKFDEDKIEDIAFAGNGCTISTASSSMMTDAVIGKSKEEALALADIFSEMVQGQE
                     NPAQKELGEAELLAGVAKFPQRIKCSTLAWNALKEAIKRSANAQHLTDQNVKEGKNV"
     gene            complement(4770..5996)
                     /locus_tag="VGT87_000193"
     CDS             complement(4770..5996)
                     /locus_tag="VGT87_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEQ3813085.1"
                     /translation="MLDAKDIKQDFQILNQQVNDEPLVYLDNAATTQKPALVLEALQS
                     YYQEDNANVHRGVHTLAERATHKYEASRQQVADFIHAKSSKEVLFTRGTTTSLNWVAR
                     FAEQVLTPEDEVLISIMEHHANIIPWQQACQKTGARLVYVYLKDGQLDMDDLANKLTT
                     KTRFVSLVHVSNVLGCINPIKEIAKLAHAKGAYLVVDGAQSVPHLAIDVQDLDCDFFA
                     FSAHKMLGPTGLGVLYGKEELLNQVEPLEFGGEMIDFVYEQEATWKELPWKFEAGTPH
                     IAGAIGLSAAISYLQRLGMADIHAHEAELIAYVLPKLEAIEGLTIYGPSQPSARSGLI
                     SFNLDDLHPHDLATALDYEGVAVRAGHHCAQPLLSYLGVPATVRASFYIYNTKADCDR
                     LVEAILKAKEFFNGTL"
     gene            complement(6027..7289)
                     /gene="sufD"
                     /locus_tag="VGT87_000194"
     CDS             complement(6027..7289)
                     /gene="sufD"
                     /locus_tag="VGT87_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEQ3813086.1"
                     /translation="MTKEKLVAFSQVHAEPAWLQERRLAALEAIPELELPTIERVKFH
                     RWNLGDGTLTENESLASVPDFIAIGDNPKLVQVGTQTVLEQLPMALIDKGIVFSDFYT
                     ALEEIPEVIEAHFGQALAFDEDKLAAYHTAYFNSAAVLYVPDHLEITTPIEAIFLQDS
                     DSDVPFNKHVLVIAGKESKFTYLERFESIGNATQKTSANISVEVIAQAGSQIKFSAID
                     RLGPSVTTYISRRGRLEKDANIDWALAVMNEGNVIADFDSDLIGQGSQADLKVVAASS
                     GRQVQGIDTRVTNYGQRTVGHILQHGVILERGTLTFNGIGHILKGAKGADAQQESRVL
                     MLSDQARADANPILLIDENEVTAGHAASIGQVDPEDMYYLMSRGLNQETAERLVIRGF
                     LGAVIAEIPIPSVRQEIIKVLDEKLLNR"
     gene            complement(7384..8154)
                     /gene="sufC"
                     /locus_tag="VGT87_000195"
     CDS             complement(7384..8154)
                     /gene="sufC"
                     /locus_tag="VGT87_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEQ3813087.1"
                     /translation="MSILEINNLHVSIEGKEILKGVNLTLKTGEVAAIMGPNGTGKST
                     LSAAIMGNPNYEVTQGQILLDGVNILDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAMNAGKADEDKISVRDFITKLDEKMALLGMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPKFALLDEIDSGLDIDALKVVSKGVNEMRGKDFGAMIITHYQRLLNYITPDLVHV
                     MMDGRIVLSGDAALATRLEKEGYAGIAQDLGIEYKEES"
     gene            complement(8276..9445)
                     /locus_tag="VGT87_000196"
     CDS             complement(8276..9445)
                     /locus_tag="VGT87_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEQ3813088.1"
                     /translation="MFSFTIDYVLVLIGALLMSLFLTPLVRFLAFRVGAVDNPNARRV
                     NKVPMPTSGGLAIFMSFLVASLGLIPIASKGAMFFGQTYFSYILPVVIGATVITLTGF
                     LDDLYELSPKLKMFGILIGAVIVWAFTDFKFDSFKIPFGGPLLVFGPFLTLFLTVLWI
                     VSITNAINLIDGLDGLVSGVSIISLVTMAIVSYFFLPQKDFFLTLTILVLIAAIAGFF
                     PYNYHPAMIYLGDTGALFIGFMIGVLSLQGLKNSTAVAVVTPVIILGVPIMDTIVAII
                     RRSLSGQKFYEPDKMHLHHRLLSMGFTHRGAVLVVYGITMLFSLISLLLNVSSRIGGV
                     LLMLGLLFGLEVFIEGLEIWGEKRTPLFNLLKFIGNSDYRQAMLLKWKEKKDLKH"
     gene            complement(9452..10213)
                     /gene="mecA"
                     /locus_tag="VGT87_000197"
     CDS             complement(9452..10213)
                     /gene="mecA"
                     /locus_tag="VGT87_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEQ3813089.1"
                     /translation="MEMKQISETTLKITISMDDLEERGMELKDFLIPQEKTEEFFYSV
                     MDELDLPDNFKDSGMLSFRVTPRKDRLDVFVTKSEINKDINLEDLAEFGDMSQMTPED
                     FFKSLEQSMREKGDVKAHEKLEKIEEIMEDVVEATLANQSEAVDPSTNHESEPLDYVH
                     YVLDFSTITEAVAFAKTIDFSIEASELYKGSNCYHMTILLDVQQQPSYFANVMYARLI
                     EHANPGSKTRAYLQEHGLQLMLDGAVEQLQKIELG"
     gene            complement(10359..11198)
                     /locus_tag="VGT87_000198"
     CDS             complement(10359..11198)
                     /locus_tag="VGT87_000198"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEQ3813090.1"
                     /translation="MLIIELLKAIFFGIIEGITEWLPVSSTGHLILVQEFIRLNQDKA
                     FIEMFNIVIQLGAIIAVMLIYFERLNPFQPGKTAREVQLTWQLWLKVVIACIPSILIA
                     VPLDNWFEAHFYFMVPIAIALIVYGIAFIWIEKQNAQQEPAVTDLARMSYKTAFFIGC
                     FQVLSIVPGTSRSGATILGAIILGTSRTVAADFTFFLAIPTMFGYSGLKAVKFFLDGH
                     HLDFAQVLILLVASLTAFVVSLLAIRFLTHYVKKHDFTIFGKYRIVLGSLLLIYSFFK
                     FVF"
     gene            complement(11264..13189)
                     /locus_tag="VGT87_000199"
     CDS             complement(11264..13189)
                     /locus_tag="VGT87_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEQ3813091.1"
                     /translation="MKKILMTLVLCFSLLGIRIKAADVDYSITNYEGQLLLSKENTAR
                     FEQKVTYQFDTSYNGQYISLGRTGHLPAGFAIDQKPKVEVYQNGQQVPVSQEFSDLGD
                     GYRLKLYNAGQAGDKVDVKVIWQLHHLLTAYQDVAELNWTPISDWDKILEKVSLTVTT
                     PTDIQDSNLWAHRGYYQKKPQVLKEGNSRYQINAKNVSGQLELHAYWDKKALLGEEPV
                     DVSTSKKNKIVALETKISRRRALLQLLFGKVIPLVEVGFLLWQLIQFTRLKKQFNRYH
                     LANHTDHSYEVPEDLSPLVLAQAIYGQSFAYLSPTASESQKLLIPKGVTFEALVQATL
                     LDLIDQKVLLLTKEEGKAYLEISQLDRVTDEEAAFLDMAFGNKVTLPVDQLFSQYHYD
                     ADTIKQLKKTYKGKKLEQEVRQSSEQVIKAMKKASAAITNNVLETIKKLNLPDTYRQM
                     TPAEKRKSNSVQGLGCLLLILNSGLLIYLAIKESGLALIYLALMVLTMCLGFYISLKL
                     DQYKKLGIETPEGGVRLHQWQSFKNMIRDIDKFEDVAIEGLVVWNRVLVYATLFGYAK
                     KVERYLKVHRIALPEVYQAVRPGELSMVMYATTPTFVSSLSSATTSSNFSVSSGGGIS
                     GGGGFSGGGGGGGGGAF"
     gene            13387..14955
                     /locus_tag="VGT87_000200"
     CDS             13387..14955
                     /locus_tag="VGT87_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEQ3813092.1"
                     /translation="MKKLILSCLVALALLFGGMSRAQANQYLRVGMEAAYAPFNWTQD
                     DASNGAVPIEGTSQYANGYDVQVAKKVAKAMNKELLVVKTSWTGLIPALTSGKIDMIA
                     AGMSPTKERRNEISFSNSYYTSQPVLVVTANGKYAEAKSLKDFSGAKVTAQQGVWHVN
                     LLTQLKGAKLQTPMGDFSQMRQALTSGVIDAYISERPEAMTAEAADSRLKMITLKKGF
                     AVAESDAAIAVGMKKNDDRMATVNQVLEGFSQTDRMALMDDMVTKQPVEKKAEDAKAS
                     FLGQMWAIFKGNWKQFLRGTGMTLLISMVGTITGLFIGLLIGIFRTAPKAKHKVAALG
                     QKLFGWLLTIYIEIFRGTPMIVQSMVIYYGTAQAFGISIDRTLAAIFIVSINTGAYMS
                     EIVRGGIFAVDKGQFEAATALGFTHGQTMRKIVLPQVVRNILPATGNEFVINIKDTSV
                     LNVISVVELYFSGNTVATQTYQYFQTFTIIAIIYFVLTFTVTRILRHIERRFDADTYT
                     TGANQMQIAEVSNV"
     gene            14948..15688
                     /locus_tag="VGT87_000201"
     CDS             14948..15688
                     /locus_tag="VGT87_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEQ3813093.1"
                     /translation="MSNSIIEIKNLKKSYGSNEVLKDISLSVNKGEVISIIGSSGSGK
                     STLLRSINLLEEPSAGQILFHGEDVLAEHYNLTHYREKLGMVFQSFNLFENLNVLENA
                     IVAQTTVLKRDRAQAEQIAKENLNAVGMTEQYWQAKPKQLSGGQKQRVAIARALSVNP
                     EAMLFDEPTSALDPEMVGEVLKTMQDLAKSGLTMIIVTHEMEFARDVSDRIIFMDKGL
                     IAEEGSPQQIFENPTQDRTKEFLQRFLK"
     gene            15830..15946
                     /locus_tag="VGT87_000202"
     CDS             15830..15946
                     /locus_tag="VGT87_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEQ3813094.1"
                     /translation="MAEKTDLSSAYRRLKSPNIKTRKRALKIIHEYKRYGKK"
     gene            complement(16172..17182)
                     /gene="gap"
                     /locus_tag="VGT87_000203"
     CDS             complement(16172..17182)
                     /gene="gap"
                     /locus_tag="VGT87_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEQ3813095.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNIEGVEVTRINDLTDPNMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGNFIKVSAERDPENIDWATDGVEIVLEATGFFAK
                     KEAAEKHLHANGAKKVVITAPGGNDVKTVVFNTNHDILDGTETVISGASCTTNCLAPM
                     AKALHDAFGIQKGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAI
                     GLVIPELNGKLDGAAQRVPVPTGSVTELVVTLDKNVSVDEINAAMKAASNDSFGYTED
                     PIVSSDIVGVSYGSLFDATQTKVMEVDGSQLVKVVSWYDNEMSYTAQLVRTLEYFAKI
                     AK"
     gene            complement(17530..19608)
                     /gene="fusA"
                     /locus_tag="VGT87_000204"
     CDS             complement(17530..19608)
                     /gene="fusA"
                     /locus_tag="VGT87_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEQ3813096.1"
                     /translation="MAREFSLAKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWDGHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVQTLHDRLQANAH
                     PIQLPIGAEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPEEYLEQAQEYREKLIEAV
                     AETDEDLMMKYLEGEEITNDELIAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVIA
                     YLPSPLDIPAIKGVNPDTDAEEERPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     NSGSYVMNTSKGKRERIGRILQMHANSRQEIETVYAGDIAAAVGLKDTTTGDSLTDEK
                     AKVILESIEVPEPVIQLMVEPKSKADQDKMGVALQKLAEEDPTFRVETNVETGETVIA
                     GMGELHLDVLVDRMKREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLIESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLALKEAAKSAQPAILEPMMLVTITAPEDNLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSATQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGE"
     gene            complement(20092..20562)
                     /gene="rpsG"
                     /locus_tag="VGT87_000205"
     CDS             complement(20092..20562)
                     /gene="rpsG"
                     /locus_tag="VGT87_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEQ3813097.1"
                     /translation="MSRKNQAPKREVLPDPLYNSKIVTRLINRVMLDGKRGTAATIVY
                     DAFSAIKEATGNDALEVFETAMDNIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNASRARGEHTMKDRLAKEIMDAANNTGASVKKREDTHKMAEANRAFAHFRW"
     gene            complement(20583..20996)
                     /gene="rpsL"
                     /locus_tag="VGT87_000206"
     CDS             complement(20583..20996)
                     /gene="rpsL"
                     /locus_tag="VGT87_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEQ3813098.1"
                     /translation="MPTINQLVRKPRKSKIEKSDSPALNIGYNSHKKVQTKMAAPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLIRGGRVKD
                     LPGVRYHIVRGALDTAGVADRKQGRSKYGAKRPKG"
     gene            complement(21204..23825)
                     /locus_tag="VGT87_000207"
     CDS             complement(21204..23825)
                     /locus_tag="VGT87_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC10/PgrA surface exclusion domain-containing
                     protein"
                     /protein_id="HEQ3813099.1"
                     /translation="MDLEQTKPNQVKQKIALTSTIALLSASVGVSHQVKADDRASEET
                     KASNTHDDSLPKPETIQEAKATIEAVEKTLSQQKTKLTELATALTKTTAEINHLKEQQ
                     DNEQKALTSAQEIYTNTLASSEETLLAQGAEHQRELTATETELHNAQADQHSKETALS
                     EQKASISAETTRAQDLVEQVKTSEQNIAKLNAMISNPDAITKAAQTANDNTKALSSEL
                     EKAKADLENQKAKVKKQLTEELAAQKAALAEKEAELSRLKSSAPSTQDSIVGNNTMKA
                     PQGYPLEELKKLEASGYIGSASYNNYYKEHADQIIAKASPGNQLNQYQDIPADRNRFV
                     DPDNLTPEVQNELAQFAAHMINSVRRQLGLPPVTVTAGSQEFARLLSTSYKKTHGNTR
                     PSFVYGQPGVSGHYGVGPHDKTIIEDSAGASGLIRNDDNMYENIGAFNDVHTVNGIKR
                     GIYDSIKYMLFTDHLHGNTYGHAINFLRVDKRNPNAPVYLGFSTSNVGSLNEHFVMFP
                     ESNIANHQRFNKTPIKAVGSTKDYAQRVGTVSDTIAAIKGKVSSLENRLSAIHQEADI
                     MAAQAKVSQLQGKLASTLKQSDSLNLQVRQLNDTKGSLRTELLAAKAKQAQLEATRDQ
                     SLAKLASLKAALHQTEALAEQAAARVTALVAKKAHLQYLRDFKLNPNRLQVIRERIDN
                     TKQDLAKTTSSLLNAQEALAALQAKQSSLEATIATTEHQLTLLKTLANEKEYRHLDED
                     IATVPDLQVAPPLTGVKPLSYSKIDTTPLVQEMVKETKQLLEASARLAAENTSLVAEA
                     LVGQTSEMVASNAIVSKITSSITQPSSKTSYGSGSSTTSNLISDVDESTQRALKAGVV
                     MLAAVGLTGFRFRKESR"
     gene            complement(23816..24655)
                     /gene="purR"
                     /locus_tag="VGT87_000208"
     CDS             complement(23816..24655)
                     /gene="purR"
                     /locus_tag="VGT87_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEQ3813100.1"
                     /translation="MKLRRSERMVVISNYLINNPYKLTSLNTFATKYEAAKSSISEDI
                     AIIKKAFEEANIGDIDTLTGASGGVIFTPSISETEARTIVEDLCQRLSESDRILPGGY
                     IYLSDLLSTPKILQNIGRIIANAFKGEKIDAVMTVATKGVPLANAVANILSVPFVIVR
                     RDLKITEGSTVSVNYASASSDRIEKMFLSKRSLKPNSRVLIVDDFLKGGGTITGMISL
                     LTEFDSTLVGVAVFAENAQSEREQMTFKSLLKVSEIDVKNNNVVVEVGNIFDKWKKER
                     EWT"
     gene            complement(24922..25860)
                     /locus_tag="VGT87_000209"
     CDS             complement(24922..25860)
                     /locus_tag="VGT87_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEQ3813101.1"
                     /translation="MKINQMKKDQLFEGFYLIKSAEVRKTRAGKDFISLTFQDDTGEI
                     SGNLWDAQPYNVEEFTAGKVVFMKGRREVYNGTPQVNQITLRNVRPGEPNDPKDFKEK
                     APVSVEEVRDYLEQMLFKIENATWQRIVRALYRKYDKEFYTYPAAKTNHHAFESGLAY
                     HTATMVRLADSIGDIYPDLNKSLLFAGIMLHDLAKVIELTGPDNTEYTVRGNLIGHIS
                     LINEEITKVISELQIDDTKEEVIVLRHVILSHHGQLEYGSPVRPRIMEAEIIHMIDNI
                     DANMMMMTTALSRVSEGEMTNRIFAMDNRSFYKPNY"
     gene            complement(25850..27121)
                     /locus_tag="VGT87_000210"
     CDS             complement(25850..27121)
                     /locus_tag="VGT87_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEQ3813102.1"
                     /translation="MDLILFLLVLVLLGLGAYLLFKVNGLQHQLAQTLEGNADNLSDQ
                     MTYQLDTANKQQLLELTQLMNRQQADLYQQLTDIRDVLHRSLSDSRDRSDKRLEKINQ
                     QVNQSLKNMQESNEKRLEEMRQIVEEKLEETLKNRLHASFDSVSKQLESVNKGLGEMR
                     SVAQDVGTLNKVLSNTKTRGILGELQLGQIIEDIMTSSQYEREFVTVSGSSERVEYAI
                     KLPGNGQGGYIYLPIDSKFPLEDYYRLEDAYEVGDKLAIEASRKALLAAIKRFAKDIH
                     KKYLNPPETTNFGVMFLPTEGLYSEVVRNAAFFDSLRREENIVVAGPSTLSALLNSLS
                     VGFKTLNIQKNADDISKILGNVKLEFDKFGGLLAKAQKQMNTANNTLDQLISTRTNAI
                     VRALNTVETYQDQATKSLLNMPLLEEENNEN"
     gene            complement(27123..27755)
                     /locus_tag="VGT87_000211"
     CDS             complement(27123..27755)
                     /locus_tag="VGT87_000211"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEQ3813103.1"
                     /translation="MSKVALFAGGDLSYISRDFDYFVGIDRGSLFLLENGLPLNMAVG
                     DFDSVSQKAFTDIKEKAELLITANPEKNDTDTELALKEVFARFPEAEVTIFGAFGGRM
                     DHLLSNIFLPSDPGIAPFMAQIALRDQQNMITYRPAGQHLIHQEEGMTYVAFMAEGEA
                     DLTITGAKFELTQDNFFKKKIYSSNAFIHQPITVSLPSGYLIIIQSKDWS"
     gene            complement(27748..28410)
                     /locus_tag="VGT87_000212"
     CDS             complement(27748..28410)
                     /locus_tag="VGT87_000212"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEQ3813104.1"
                     /translation="MSTLKIAPSILAADYANFASELARIEETDAEYVHIDIMDGQFVP
                     NISFGADVVASMRKHSKLVFDCHLMVVDPERYVEAFAQAGADIMTIHTESTRHIHGAL
                     QKIKAAGMKAGVVINPGTPATALEPLLDLVDQVLIMTVNPGFGGQAFIPECLEKVATV
                     AKWRDEKGLSFDIEVDGGVDNKTIRACYEAGANVFVAGSYLFKASDLVSQVQTLRTAL
                     NV"
     gene            complement(28420..29292)
                     /gene="rsgA"
                     /locus_tag="VGT87_000213"
     CDS             complement(28420..29292)
                     /gene="rsgA"
                     /locus_tag="VGT87_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEQ3813105.1"
                     /translation="MQGKIIKSLAGFYYVESEGQVYQTRARGNFRKRGETPYVGDIVD
                     FSAEDNSEGYILAIHPRKNSLVRPPIVNIDQAVVIMSAKEPEFNSNLLDRFLILLEHK
                     AIHPVVYISKMDLLDSPEEIKAIGRQYQAIGYNFVTSLEELLPLLADKITVFMGQTGV
                     GKSTLLNRIAPELALETGEISDSLGRGRHTTRAVSFYNTHGGKIADTPGFSSLDYDIA
                     NAEDLNEAFPELRRLSHECKFRSCTHTHEPKCAVKAALEMGELWPVRYEHYLQFLSEI
                     ENRRETYKKVIKRK"
     gene            complement(29716..30588)
                     /gene="rsmA"
                     /locus_tag="VGT87_000214"
     CDS             complement(29716..30588)
                     /gene="rsmA"
                     /locus_tag="VGT87_000214"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEQ3813106.1"
                     /translation="MRIADYSVTKTVLDRHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DQNVNVIEIGPGIGALTEFLAENAAEVMAFEIDDRLVPILADTLRDFDNVQVVNQDIL
                     KADLQTQIKQFKNPDLPIKVVANLPYYITTPILMHLIESKIPFQEFVVMMQREVADRI
                     SAEPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRDQPLIEVED
                     EDFFFRVSRLSFVHRRKTLWNNLTSHFGKSEDIKAKLEKGLALADIKPSIRGEALSIQ
                     DFGKLADALKEVGL"
     gene            complement(30715..31305)
                     /gene="rnmV"
                     /locus_tag="VGT87_000215"
     CDS             complement(30715..31305)
                     /gene="rnmV"
                     /locus_tag="VGT87_000215"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEQ3813107.1"
                     /translation="MQRGYSSLTEKINIQEVLVVEGKDDTANLRRFYEVDTYETRGSA
                     ITEEDLERINRLNDLRGVIVLTDPDYNGERIRKLIMAAVPTARHAFLNRNEATPSSKS
                     KGRSLGVEHANFEDLQKALAHVTQQYDDESYFDIRQTDLIRLGLLMASDSRKRREYLG
                     EKLRIGYANGKQLLKRLELFGITLAEVEEVMEAYEE"
     gene            complement(31277..32068)
                     /locus_tag="VGT87_000216"
     CDS             complement(31277..32068)
                     /locus_tag="VGT87_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEQ3813108.1"
                     /translation="MTNKVEKLTIFDTHTHLNVAEFQGHETEELTLAQEMGVAYHNVV
                     GFDQATISGALALADQYPNVYATIGWHPTEAGSYSEAVEESIVSQLSHPKVIALGEIG
                     LDYYWMEDPKEVQIEVFKRQMQLAKDHDLPFVVHTRDALEDTYEIIKAAGVGPRGGIM
                     HSYSGSLEMARRFVDLGMMISFSGVVTFKKALDIQEAAQHLPLDKIVVETDAPYLTPV
                     PKRGKQNHTAYTRYVVDKIAELRGMTVEEVAKATTANAKRVFKLD"
     gene            32353..33183
                     /locus_tag="VGT87_000217"
     CDS             32353..33183
                     /locus_tag="VGT87_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEQ3813109.1"
                     /translation="MPKNHRLITKIEASLEHMTSLEKGIAHFFITTDLTPQELTASEI
                     VKRLHISQAALTRFAKKCGFTGYRAFAFDYLHSLQESQETFQSIHLELTKRVLMDYDA
                     LINKTYELVNEEKLLNLAKLIDASERVYFFGKGSSGLVAREMKLRFMRLGLICDAYSD
                     TDGFTWANSLVNDNCLVFGFSLSGKTNSVITALHQASQRGAKTVLLTTDNQTEFDDSL
                     DIIPVSSTHQLHYGNRVSPQFPLLIMMDIIYAYVLAIDKPHKEKIFKNTIIDKENNDL
                     "
     gene            complement(33294..34232)
                     /locus_tag="VGT87_000218"
     CDS             complement(33294..34232)
                     /locus_tag="VGT87_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEQ3813110.1"
                     /translation="MKHYLAIDIGGTAIKYGLISETGDLLEKEEMATEAYKGGPSILE
                     KVKGLVKTYQDQMDLAGVAISSAGMVNPDKGEIFYAGPQIPNYAGTQFKNEIEETFGL
                     PCEVENDVNCAGLAEAISGSAKDYPVALCLTIGTGIGGCLLFNSQVFHGSSHSACEVG
                     YLHLSDGQFQDLASTTALVQEVALAYGDDISQWDGRRIFEQAKAGDAICIAAISKQVD
                     YLGQGIANICYVVNPNVVVLGGGIMAQKDYLADKLKTALDSYLVSSLAKKTQLKFASH
                     GNNAGILGAYYHFKQKNERSCSFITPEKIENNLASS"
     gene            complement(34254..35168)
                     /locus_tag="VGT87_000219"
     CDS             complement(34254..35168)
                     /locus_tag="VGT87_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEQ3813111.1"
                     /translation="MTDLTKYQGIIPAFYACYDDQGAISPERVRALTQYYIDKGVQGL
                     YINGSSGECIYQSVFDRQLVLENVMAVAKGKLTIINHVACNNTKDSIELAAHSERLGV
                     DAIAAIPPIYFRLPEYAVADYWNAISSAAPHTDFIIYNIPQLAGVALTPSLYKTMLAN
                     KRVIGVKNSSMPVQDIQTFCAIGGDDHIVFNGPDEQFLGGRLMGAAAGIGGTYGAMPE
                     LFLRLNQLIVDKDLEKAKTFQYTINEIIGVLVSAHGNMYGVIKEVLRINEGLDIGSVR
                     SPLAELVEEDRVICQRAAALINQAKETF"
     gene            complement(35180..35842)
                     /locus_tag="VGT87_000220"
     CDS             complement(35180..35842)
                     /locus_tag="VGT87_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEQ3813112.1"
                     /translation="MTSKKQGLLHSLFNLDSKWMRASAALFDLLVFNLLFVLSCLPLL
                     TIGVAKMALYASLLDWREGQVSQLVTTYSSHFKHYFKSGMRLGLIELLIMSICVLDLF
                     LIRNQSGLVFQGFKVLCIAVLFLVVILFLYAYPQAVRKELSLSTLFKRTFLLAGLFFP
                     WSFAFLALICLTIFSLQLSLLTLFGGVSLLAIIGISSLTYLYLIIMESLLRRFPLNND
                     IE"
     gene            complement(35886..36341)
                     /locus_tag="VGT87_000221"
     CDS             complement(35886..36341)
                     /locus_tag="VGT87_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEQ3813113.1"
                     /translation="MIYDRLKNISRYRGIHPHLDAAITFLQTTDLRQLAEGKYPILGE
                     KVFAVIQRNQLSKADNSLLEYHKRYADCHLLLAGNECIRYGIGNQAEVVPFEQEADIG
                     FVTCDRTYDLDLVDDSFAYFFPNEPHQPNNFNQAGEAVTKCLIKVLMAD"
     gene            complement(36361..37191)
                     /locus_tag="VGT87_000222"
     CDS             complement(36361..37191)
                     /locus_tag="VGT87_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEQ3813114.1"
                     /translation="MKKKKLTASDVLTTVILCILTILFIFPFYWIMTGAFKAQADTIM
                     IPPQWWPKAPTIENFKALVVQNPALKWLWNSVFISVATMFLVCGTSSLAGYALAKKRF
                     YGQRLLFSIFIAAMALPKQVVLVPLVRIVNFMGIHDTLAAVILPLVGWPFGVFLMKQF
                     SENIPTELLESAKIDGCGEIRTFFNVAFPIVKPGFAALAIFTFINTWNDYFMQLVMLT
                     SRENLTISLGVATMQAEMATNYGLIMAGAAMAAVPIVTVFLVFQKSFTQGITMGAVKG
                     "
     gene            complement(37204..38091)
                     /locus_tag="VGT87_000223"
     CDS             complement(37204..38091)
                     /locus_tag="VGT87_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEQ3813115.1"
                     /translation="MNVNKLKMRETLISYAFLAPVLVFFVIFVLIPMIMGFVTSFFNY
                     SMTDFTFVGFANYARMFQDPIFMKSLINTLIIVIGSVPVVVFFSLFVAAKTYDKNVVA
                     RSFYRAVFFLPVVTGSVAVTVVWKWIYDPMSGILNYVLKYAHVIEQNISWLGDKHWAL
                     LAIIVILLTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWNIKWPSLLPTTL
                     YIAVITTINSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFKLSEYGYANTMGVFLAV
                     MIAIISFAQFKILGNDVEY"
     gene            complement(38196..39515)
                     /locus_tag="VGT87_000224"
     CDS             complement(38196..39515)
                     /locus_tag="VGT87_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEQ3813116.1"
                     /translation="MNMKKFASLAMLGASVLGLAACGGKSQKEASESKSDAAKTEITW
                     WAFPVFTQEKAEDGVGTYEKKLIAAFEKANPEIKVKLETIDFTSGPEKITTAIEAGTA
                     PDVLFDAPGRIIQYGKNGKLADLNDLFTKEFTKDVNNDKLIQASKAGDTAYMYPISSA
                     PFYMALNKKMLKDAGVLDLVKEGWTTDDFEKVLKALKDKGYNPGSFFANGQGGDQGPR
                     AFFANLYSSHITDDKVTKYTTDDANSIKAMTKISNWIKDGLMMNGSQYDGSADIQNFA
                     NGQTSFTILWAPAQPGIQAKLLEASKVDYLEIPFPSDDGKPELEYLVNGFAVFNNKDE
                     QKVAASKKFIQFIADDKEWGPKNVVRTGAFPVRTSYGDLYKDKRMKKIAEWTKFYSPY
                     YNTIDGFAEMRTLWFPMVQAVSNGDEKPEDALKAFTEKANKTIKKTQ"
     gene            complement(39564..>40263)
                     /locus_tag="VGT87_000225"
     CDS             complement(39564..>40263)
                     /locus_tag="VGT87_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEQ3813117.1"
                     /translation="DKPTKEKLMEQLKGEIIVSCQALPGEPLYSETGGIMPLLAKAAQ
                     EAGAVGIRANSVRDIKEIQAITDLPIIGIIKKDYPPQEPFITATMTEVDQLAALNIAV
                     IAMDCTKRDRHDGLDIASFIRQVKEKYPNQLLMADISTFDEGLVAHQAGIDFVGTTLS
                     GYTPYSRQEAGPDVALIEALCKAGIAVIAEGKIHSPEEAKKINDLGVAGIVIGGAITR
                     PKEIAERFIEALKS"
BASE COUNT        12014 a         8665 c         7420 g        12164 t
ORIGIN      
        1 acactccttt tttaatgagg tttcaattag tttaataaag attgtcattc tctcatggta
       61 gcgttttatt acatcactac tcttgtgagg taagtcatta ccttagacaa aaatatggta
      121 taattaaaaa aataatactt gagtaaggat aatctataaa tgatcaaacg attaatttcc
      181 ctagtggtca tcgccttatt ttttgcagca agcactgtta gcggtgaaga gtattcggta
      241 actgctaagc atgcgattgc cgttgacctt gaaagtggca aagttttata cgaaaaagat
      301 gctaaagaag ttgtccctgt cgcctcagtc agtaagctct tgacaaccta tctggtttac
      361 aaagaagttt ctaagggcaa gctaaattgg gatagtcctg taactatttc taactaccct
      421 tatgaactca ctacaaacta tactattagt aacgttcctc ttgataagag aaaatatacc
      481 gttaaagaac ttttaagtgc gttagttgtt aataacgcca atagccccgc tattgcttta
      541 gctgaaaaaa taggcggaac cgaacccaaa tttgttgaca aaatgaaaaa acaattaaga
      601 caatggggca tttccgatgc aaaggtcgtc aattcaactg gcttaactaa ccatttttta
      661 ggagctaata cttatcctaa tacagaacca gatgatgaaa attgtttttg cgccactgat
      721 ttagctatta ttgccaggca tctcttatta gaatttccag aagtactgaa attatctagc
      781 aaatcctcca ctatttttga tggacaaacc atttacagtt ataattacat gcttaaaggc
      841 atgccttgtt atcgagaagg cgtggatggt ctttttgttg gttattctaa aaaagccggt
      901 gcttcttttg tagctactag tgtcgaaaat caaatgaggg ttattacagt agttttaaat
      961 gctgatcaaa gccacgagga tgatttagct atatttaaaa caaccaatca attgttgcag
     1021 taccttttaa ttaattttca aaaagtccag ttaattgaaa ataataaacc agtaaaaacg
     1081 ttatctgtct tagacagtcc tgaaaaaact gtcaaacttg tagcccaaaa tagtttattt
     1141 tttatcaaac caatacatac aaagaccaaa aataccgtcc atattactaa gaaatcatcc
     1201 acaatgatcg cacctctatc aaagggacaa gtcttaggta gagcaaccct tcaagataaa
     1261 catcttattg gacaaggtta tctggatact cctccttcta tcaatcttat ccttcaaaaa
     1321 aacatttcta aaagtttctt tttaaaggtc tggtggaacc gttttgtgag gtatgtcaat
     1381 acctctttat agaataacac aatgcttttt gtatgacatt gactgacaga gtacttctgc
     1441 agtctttttc atttttattt aagcaaacta ggccaatcct aacaattttt caaaaaaact
     1501 tcacattgta ttagcaaagt gatatactga aaatgcatta catttaaagg agtcggaaat
     1561 tgaccaaaag aattatacta actattttta cctttatttg cttttcgatg atgcctcttg
     1621 tccacgccga agatgtcatg gatattacaa ggcaagcagg ctacacagtt agtgaggtta
     1681 atcgaccaaa atcttctatt gtcgttgatg caaatagttc agatattctt tggcaagata
     1741 atattgatat tcctagggat cctgctagta tgtctaagat gtttacgctt tacattttat
     1801 ttgaagagct cgctaaggga aaaattacga tggacactac tattacggca actccaaccg
     1861 atcaagctat cgctaacatt tatgagatta gcaataataa tatcgtcgct ggagtagctt
     1921 atcctattcg tgatcttatt actatgacag ctgtgccttc atcaaatgca gcaactgtta
     1981 tgattgccaa ttatttatca aacaatgatg cttctgcgtt tatcgatcgt attaatgcca
     2041 cagccaaaca attaggcatg actaacactc atttttcaaa cgctagtggt gcagcagcac
     2101 aagcttttca aggttattat aatcctacta aatatgattt atctgcttca aatattacga
     2161 ccgctcgaga tttgtccaag ttactctatg ccttcttaaa aaaataccct gaaattatct
     2221 cctttacaaa taaatctgtt gtgcacacta tggtaggaac gccatatgaa gaagaatttc
     2281 acacctataa ccactctctc ccagataatc aatttggtat aaaaggagtt gatggattaa
     2341 aaacaggttc tagtcccagc gctgctttta acgccatgat aactgccaaa agaggcaaaa
     2401 ctcgactcat tactattgtc atgggggtcg gagattggtc agatcaaaac ggagagtttt
     2461 atagacatcc tttcgttaat gccttgactg agaaagggtt taaagatagt aagaccttat
     2521 ccaaaaaagc acgccaaaaa ctcgaaaaat tagtgccaca aaccaaaaaa gaaacatcac
     2581 ctaaacagca gcacttcaaa gccactaaaa agcaatcgta cttggaaaga gtcgaagatt
     2641 ttatgaatca caaccatact ttcttgctta tttgcttagc tatctttata attactatcc
     2701 ttttactaag cctcgttgtc tttgcaatgg gacgccaata atgaattatc aaaaacctct
     2761 gtcaaagcac agaggttttt gatatgttat cttacgtgtt taaggcaact aaaaactaac
     2821 cacttggggt tagtactaca actcataaaa tcctatctca aagatctttc agatggataa
     2881 gagaaaatgc aattaaccga cagaaccttc catctcatag gaaatcaatc gattgagttc
     2941 gaccgcgtat tccattggca attctttggt aaaaggctcc acaaatccca tgacaatcat
     3001 ctctgtggct tcgctttctg ataagccacg gctcatgaga tagtagagtt gttcttcaga
     3061 aatcttagac actttggctt catgttctaa cgccacctgt gaattatgaa tttcgttaaa
     3121 tgggatcgta tctgatttag aaatatcgtc catcaaaata gtatcacatt caatatggga
     3181 aacggatttt ttagattgct tgttaaaggt cacttggcca cgataatcta ctttaccacc
     3241 agacttggca attgattttg acacaattga tgatgatgta tgtggagcat tgtgaatcat
     3301 tttagcccct gtatcttggt gttggcctgc gttagcaaag gcaatagaca gcatggtgcc
     3361 acgcgcacct ggcccatcaa gataaacaga agggtacttc atggtggttt tagctcctag
     3421 attgccatca atccattcca ctgttgcatc cgtaagggcg cgtgcacgtt ttgttactag
     3481 attatacaca ttgtctgacc agttttgaat ggtggtataa cgcatgtatg caccgtcaag
     3541 cgcaaagatt tcaacgatag cagcatgtaa gctgttactt gaataagtgg gagctgtaca
     3601 tccttcaaca taatgaacac ttgctccttc atcaacaata atcaatgtcc gctcaaattg
     3661 accagtattt tcattgttaa tgcggaaata agtttgcaaa ggaatatcta ccttgacccc
     3721 tttaggaaca taaataaagg taccaccaga ccaaactgct gaattgaggg ctgctaattt
     3781 gttgtctgtt ggcggaacca gtttggcaaa gtattgtttg aacaaatcag gatattcttt
     3841 gagggcggaa tcggtatctg taaagataat ccctagcttt tcaaattcac ccttcatgtt
     3901 atggtaaacc acttctgact catactgagc tgatgctcca gcaagataag cacgttctgc
     3961 ttctggaatt ccaatacgat caaaggtttc ttttattttt tctggaacat catcccaaga
     4021 acgagctggc ttatcagatg ctttttgata gtaaatgata tcatcaaagt taatatctga
     4081 taagtctgct ccccaggttt gcatcggcat tttattaaag gtttccaagg attttaaacg
     4141 aaactccaac atccactcag gttcattttt ggcagctgat agttcgcgaa ctactgcctc
     4201 actcaatcct tttcctgtag aataaatggg ctctacatca tcgtgaaatc caaattggta
     4261 gtcccctaag tcaattggct ttggctctac tttctcattt atatcagaca tttttccctt
     4321 cctttacatt ttggtccgtg aggtgctgag cattggcact tcgtttaatg gcttccttga
     4381 gagcattcca agctagggta gagcatttga tacgctgtgg aaattttgca actcctgcca
     4441 acaattctgc ttcacctagc tctttttgag caggattttc ctgtccttgt accatctctg
     4501 aaaaaatatc agctaacgcg agtgcttctt ctttactttt accaataaca gcatctgtca
     4561 tcatgcttga tgaagctgtg gaaatggtac agccgttgcc tgcaaaagca atatcttcaa
     4621 ttttatcttc gtcaaattta acggtcaaag aaatcacatc accacaagtc ggattattca
     4681 gttgaacagc ctctacgcca tctagttgcc catgatgatg tggacgtttc gaatggtccg
     4741 ctaccacagc catgtataga tggttcagtt tagagagtgc cattgaaaaa ctcctttgct
     4801 tttagaattg cttcgactaa acggtcacaa tctgccttgg tgttatagat ataaaaactt
     4861 gctctaacag ttgctggtac acctaaataa ctaagaagag gttgggcgca gtggtgtcct
     4921 gctcttactg caacaccttc atagtccaag gctgttgcca agtcatgagg atgcaaatca
     4981 tccagattaa aagaaatcag accagatctt gcactaggct ggcttggtcc atatatggta
     5041 agcccctcaa tagcttctaa tttcggcaaa acataggcta ttagttctgc ttcatgcgca
     5101 tgtatatcag ccatgcctag tctctgaagg taagaaatgg ctgcgcttag cccaatagca
     5161 ccagctatgt gaggtgttcc tgcttcaaac ttccagggca attctttcca agtggcctct
     5221 tgttcgtaaa caaaatcaat catttctccg ccaaattcaa gaggctccac ttgattcaaa
     5281 agctcttctt tgccgtaaag aacacccaaa cctgttggcc ccaacatctt atgagctgaa
     5341 aaagcaaaga aatcacaatc caagtcttga acatcaatag ccaaatgtgg aaccgactgg
     5401 gcaccgtcaa caacaaggta ggctccttta gcatgtgcca gcttggcaat ttctttaatg
     5461 ggattgatgc aaccaagaac attggagaca tgtactaggc taacaaaacg tgtttttgtc
     5521 gtcaatttgt ttgccaaatc gtccatgtca agttggccat cttttaaata aacatagact
     5581 aaccttgctc ctgttttttg acaggcttgt tgccaaggaa tgatattggc atgatgctcc
     5641 ataatcgaaa tcaacacctc atcttctggc gtcaagacct gctctgcaaa ccgagcaacc
     5701 caattcaaac tggttgttgt tcctctggta aagagcactt ccttgcttga tttagcatga
     5761 ataaagtcag caacctgctg gcgactggcc tcatatttgt gcgttgcacg ttcagccaag
     5821 gtatgaactc ctcggtggac attagcatta tcttcttgat aataggattg caaagcctca
     5881 agaacaagcg ccggtttttg tgtggtggcg gcattatcca aataaacaag gggttcatca
     5941 ttgacttgtt gatttaagat ttgaaagtct tgtttgatgt cttttgcgtc tagcaatact
     6001 aacctctttc tttttggcag taggtcttaa cgattaagca atttctcatc taaaacctta
     6061 ataatctctt ggcggactga tggaatagga atttcagcga taaccgctcc taggaatcct
     6121 ctaataacca atcgttctgc tgtttcttga tttagtcctc gactcatcaa gtaatacata
     6181 tcttcagggt caacctgacc gatagaagct gcatgacctg ctgttacttc attttcatca
     6241 attaagagga ttggattggc atcggctctt gcttggtcag aaagcatcaa aacacggctt
     6301 tcttgttgag catcagctcc cttagcgcct tttagaatat gaccaatccc gttaaacgtt
     6361 aaggtgccac gttccaaaat cacaccatgc tgtaaaatat gaccaaccgt acgttgacca
     6421 tagttggtca cgcgcgtgtc aataccttgt acctgacgac cacttgaggc tgcaacaact
     6481 ttcaaatcag cttgtgagcc ctgaccaatc aaatcactgt caaaatcagc aatgacattg
     6541 ccttcattca tcacagctaa ggcccaatca atgttggcat ccttctctaa acgtcctcga
     6601 cggctaatat aggttgtcac tgaaggacct aagcggtcga tagccgagaa tttaatctgg
     6661 ctgcctgctt gagcaatcac ttctacactg atattagcgc tggtcttttg agtggcattg
     6721 ccaatagatt caaaacgctc taaataggtg aacttacttt cttttcctgc aatcactaga
     6781 acatgcttgt taaaaggaac gtcactgtca ctatcttgta agaaaatagc ttcaatagga
     6841 gttgtgattt ccaagtgatc aggaacgtag agcacggctg cgctattaaa ataagcagtg
     6901 tggtaggcag ctagtttgtc ttcatcaaaa gctaacgcct gaccaaaatg agcttcaatt
     6961 acttctggga tttcctcaag cgccgtataa aaatcactga aaacaattcc cttgtcaatt
     7021 aacgccattg gtaactgttc taagactgtt tgcgtgccta cctgaacaag ctttgggtta
     7081 tctccaatag ctataaaatc tggaacacta gctagacttt cattttctgt taaagtacca
     7141 tctcctagat tccaacggtg aaatttaacc ctttcgatgg ttggtaactc taattctggt
     7201 atggcttcta atgccgctaa acgccgttct tgcagccaag caggctcagc gtggacttgc
     7261 gaaaaagcca ctagtttttc ttttgtcatc tgtcaatcct ttgtctctgg tttttaaatt
     7321 ggctgggtat atagaacatc acctagcttt ttgtagaaag ctaaagtaat tgtctccaaa
     7381 aacttaactt tcttccttat actcgattcc cagatcctgt gcaatacctg cgtagccttc
     7441 tttttcaaga cgagtagcca aggcagcatc accagataag acaatacgac catccatcat
     7501 aacatgaact aggtctgggg taatgtaatt caacaaacgt tgatagtggg tgataatcat
     7561 agctccaaag tctttcccac gcatctcatt aaccccttta gagactactt ttagggcatc
     7621 aatatcaaga ccagaatcaa tttcatctag caaagcaaat ttaggctcta acatgagcaa
     7681 ttgaagaatt tcattgcgtt ttttctcccc accagaaaaa ccttcattga ggtaacgctc
     7741 tgccatctct tctttcatac ctaacaaagc cattttctca tcaagtttgg tgataaaatc
     7801 tcgcacggat atcttatcct catctgcctt accggcattc attgctgcac gcataaattc
     7861 agcgttggta atccctggaa tttctgaagg gtattgcatg gcgagaaaaa gtcccagtct
     7921 cgcacgctca tcaacttcta gatcgagaat attgaccccg tcaagcaaaa tctgcccttg
     7981 ggtcacttcg tagttaggat ttcccataat agctgctgaa agggtagact ttcctgtccc
     8041 atttggcccc ataatggcag ctacttcccc agttttaaga gttaggttaa cccctttcaa
     8101 aatctcttta ccttctattg aaacatgtaa attattaatc tcaagaattg acatgataca
     8161 ccccttattt tctctgtcat ctataccatc tcaagaaaag acagtgtcat ttgcactgct
     8221 ttttttataa tggtcactgc atctattata gcaaaaaaag ccttactcgg cttttttagt
     8281 gtttcaaatc cttcttttct ttccatttca gaagcattgc ttgacgataa tcactattac
     8341 caataaactt taacaagtta aacaaaggcg ttcgcttttc accccagatc tccaatcctt
     8401 caataaaaac ttctaaacca aataaaagtc caagcatcaa gaggacgcca ccaattcgac
     8461 tagaaacatt taaaagtaaa gaaataaggg aaaaaagcat ggtaatacca taaactacta
     8521 agacagctcc tcgatgagta aagcccattg ataagagtcg atgatgcagg tgcatcttat
     8581 ccggctcgta gaatttttga cctgataaac tacgtcgaat aatagccacg atggtatcca
     8641 taatgggtac accaagaata ataacaggtg tcaccacagc cacagccgtt gaatttttca
     8701 atccttgaag agacaaaacc cctatcataa agccaataaa gagggctcct gtgtcaccca
     8761 aataaatcat agctggatgg taattataag gaaaaaatcc tgcaatagca gcaatcaaga
     8821 ccaaaatggt taacgtcaaa aagaaatcct tttgaggtaa aaagaaataa gatacaatag
     8881 ccatggtcac taaactaata atagagaccc cactaaccaa accatccaaa ccgtcaatca
     8941 agttaatagc attagtgatg gaaacaatcc acaggactgt taagaataag gttaagaaag
     9001 gaccaaaaac taacaacggc cctccaaaag gaattttgaa gctatcaaat ttaaagtcgg
     9061 taaaagccca gacaatcact gcaccaatta gaataccaaa catctttaat ttaggactta
     9121 actcatacaa atcatctaaa aaaccagtaa gggtaattac tgtagcacca atcacgacag
     9181 gtaaaatata actgaagtag gtctgaccaa aaaacatggc gcctttagaa gcaatcggaa
     9241 taagtcccaa acttgctact aaaaaagaca taaaaatagc taatccacca ctggttggca
     9301 tcggaacctt attgacccgt ctggcattgg gattgtctac tgcacccaca cgaaaggcta
     9361 aaaagcgaac aagaggggtt aagaataagg acattaacaa agcccctatc agaacaagga
     9421 cataatctat tgtgaatgaa aacatactat tttatcccaa ctcaatcttt tgtaattgct
     9481 caacagcacc atcaagcatc aattgtaagc catgctcttg taaataagct cttgtttttg
     9541 atccagggtt tgcatgctca atcaatctcg catacatgac attcgcaaaa taagatggtt
     9601 gttgctgaac atctaataaa atagtcatat ggtagcagtt gctcccctta tataactctg
     9661 aagcttctat cgaaaaatca atcgttttag caaaagccac tgcctctgta atcgttgaaa
     9721 aatccaatac ataatggaca tagtctaaag gttctgactc atgattagtt gatggatcaa
     9781 ccgcttcaga ctgattagct agagtggctt caacaacatc ttccattatc tcttcaattt
     9841 tttccaattt ttcatgagct ttgacatctc ccttttcacg catggattgc tccaagctct
     9901 tgaaaaaatc ttccggagtc atttgggaca tatcaccaaa ctctgctaaa tcttcaagat
     9961 taatatcttt atttatctca gatttggtaa caaaaacatc tagacggtcc ttgcgtggag
    10021 tcactcgaaa actaagcata ccactatctt taaaattatc tggaagatcg agttcgtcca
    10081 tcacagaata gaaaaactct tctgtttttt cttgaggaat caagaaatct ttcaactcca
    10141 ttcctctttc ttctaaatcg tccatactaa ttgttatttt tagcgttgtc tcgctaattt
    10201 gtttcatttc cattctcaat acctcgcacg cgtagtcatt ttattatact agaaatgctg
    10261 taatttcgca atttatgact aactcctcgt ctacaaaagg aaatttaaaa agcgacagtc
    10321 cccaaggtct gtcgcaacat cctaaacgat atgtaagttt aaaaaacaaa tttaaagaag
    10381 ctataaataa gtaaaaggct tcctaacaca atacggtatt ttccaaaaat ggtgaaatcg
    10441 tgtttcttaa cataatgtgt caagaaccta atcgccaata aactaaccac aaaagcggtc
    10501 aagctagcca ctaaaagaat aaggacttgt gcaaaatcaa ggtgatggcc atcaagaaaa
    10561 aatttcacag cctttaatcc actatatcca aacatggtag gaatggccaa aaagaaggtg
    10621 aaatcagcgg ccactgttcg actagttcct aaaataatag ctcctaagat ggtagcccct
    10681 gatcgactag taccaggcac aatacttaag acttgaaaac aaccaatgaa gaaagctgtt
    10741 ttgtaagaca ttctagctaa gtcagtgacg gcaggttctt gttgagcatt ttgtttttca
    10801 atccagataa aggcaatccc gtagacgatt aaagcgatgg caatcggaac cataaaataa
    10861 aaatgtgctt caaaccaatt atcaagagga accgctatta aaatagaagg aatacacgca
    10921 atcaccactt ttagccataa ttgccaggta agctgtactt ctcttgctgt tttcccaggt
    10981 tggaagggat tgagacgttc aaaataaatc aacataacag cgataatcgc ccctagttga
    11041 ataacgatat taaacatttc tataaaagcc ttatcctgat ttagtcggat aaattcctgc
    11101 actaaaatga ggtgaccagt gcttgaaact ggtaaccatt ctgtgattcc ctcaatgatg
    11161 ccaaagaaaa tggcttttag caattcgatt attaacatgt tttctcctta ttaaccactc
    11221 ttattgtgat tttctaaact gtgattaatg ccaagcagct tttttaaaag gcaccgccgc
    11281 cgcctcctcc gccaccgcca gagaagccac caccaccact aatcccgcct ccggaagaga
    11341 ctgagaaatt tgaggaagtt gtagctgaag acaagctaga cacaaaggtt ggtgtcgtag
    11401 catacattac cattgataat tcacctggtc gaacagcttg gtaaacctca ggtaaggcaa
    11461 tacgatggac tttcaaatag cgttccactt tcttagcgta accaaataag gtggcataaa
    11521 ccaaaacacg gttccaaaca accaaacctt caatagcaac atcctcaaat ttgtcaatgt
    11581 ctcgtatcat gtttttaaag ctctgccatt gatgtaaccg aacacccccc tcaggtgttt
    11641 caatacccaa tttcttgtat tgatctaact tcaggctaat gtaaaagcca aggcacatcg
    11701 ttagcaccat taaagcaagg taaatgaggg ccaacccact ttctttaata gccaagtaaa
    11761 tcaataaacc actatttaga ataagcaaca aacaacctag tccctggaca ctattgcttt
    11821 ttcgtttctc tgccggggtc atttgacgat aagtatctgg taagtttaac tttttgatag
    11881 tttctaggac attgtttgta atagctgccg acgctttttt catcgcctta atcacctgct
    11941 cagacgattg acggacttct tgctcgagct ttttaccttt gtacgttttc tttaattgtt
    12001 taatggtatc agcatcataa tggtattgac taaacaattg atccaccggc aatgtcacct
    12061 tgtttccaaa agccatatct aagaaggcag cttcctcatc tgtcacacga tctaactgac
    12121 tgatttcaag ataagctttc ccttcttctt tcgttaataa aagcaccttt tggtcgatca
    12181 gatccaacaa ggtagcttga acaagggcct caaaggtcac tcctttaggg atcaataatt
    12241 tctggctttc tgatgctgta ggggataaat aggcaaaact ctgtccataa atcgcttgtg
    12301 ccaacacaag aggcgaaagg tcttcaggga cttcgtaact gtgatctgta tggttggcca
    12361 aatgataacg attgaattgt ttctttaatc ttgtaaactg tattaactgc caaagaagaa
    12421 aacctacctc taccagagga atgactttgc caaatagcag ctgtaacaag gcccttcgtc
    12481 tggaaatctt agtttctaac gctacaattt tattcttttt actcgttgaa acatcaactg
    12541 gttcttctcc caataatgct tttttatccc aataagcatg caattccaat tgccctgaga
    12601 catttttggc attaatctga tagcgactgt taccttcttt taacacttga ggtttctttt
    12661 ggtaatatcc tctgtgagcc catagattag aatcctgaat atcagttggg gtagtgacgg
    12721 tcaaactcac cttttctaat attttgtccc aatcactaat cggtgtccaa ttcagctctg
    12781 ccacgtcttg ataagctgtt aaaaggtggt gcagttgcca gataaccttg acgtcaacct
    12841 tatcaccagc ttggccagca ttataaagtt ttaaacgata gccatctcca aggtcactaa
    12901 actcttgact aacaggtacc tgttgaccat tctgatatac ctcaacttta ggcttttggt
    12961 caatagcaaa gcccgctggc aaatgacctg tccggcccag cgaaatatac tggccgttat
    13021 aagaagtgtc aaattgatag gttacttttt gctcaaaccg agcagtattt tcttttgata
    13081 acagtaactg accttcatag ttggtgatgc tatagtctac atcagctgct tttattcgta
    13141 tccctaaaag actgaagcag agcacaagcg tcatcaaaat ttttttcatg gcaacatcct
    13201 ctttctgccc tcattttacc atattttaag gagatgacaa aacctaaacc aagtccttca
    13261 tcttattttt aatcattatt ttgaatattt ttccatattt caacctattc actgataata
    13321 tattgaaata ggatgcaaat tgctgtaaaa taggtctgaa tactattttt gaaacaggag
    13381 acctatatga aaaagctaat ccttagctgt ttggtcgcct tggcccttct gtttggagga
    13441 atgagccggg ctcaagcaaa ccaatattta agagtcggaa tggaagcagc ctatgctcct
    13501 tttaactgga ctcaagatga cgcttcaaac ggggctgtcc ctattgaggg aactagccaa
    13561 tacgccaacg gttacgatgt ccaagtcgct aaaaaagtcg ctaaagctat gaacaaagaa
    13621 ctgttagtcg ttaagacctc ttggaccggt ttaattccag cattaacttc tggaaaaatc
    13681 gatatgatcg ctgctggtat gagtcctacc aaagagcgta gaaacgaaat tagcttctca
    13741 aacagctact acactagcca acctgttcta gtcgtaactg ctaatggcaa atatgctgag
    13801 gcaaaaagcc tcaaggattt ttctggagct aaagtaactg cccagcaagg cgtttggcat
    13861 gtcaatctct taactcaact aaaaggtgct aagttacaaa caccaatggg ggatttctct
    13921 caaatgcgac aagcccttac ttcgggtgtt atcgatgcct atatttctga acggcctgaa
    13981 gccatgactg ctgaagctgc tgatagccgt ttgaaaatga tcactcttaa aaaagggttt
    14041 gctgttgctg aatcagatgc tgctatcgct gtcggaatga aaaaaaatga cgatcgtatg
    14101 gcaactgtca accaagtgct tgaaggattt tctcaaacgg atcgtatggc cctgatggat
    14161 gatatggtta ccaaacaacc cgtggaaaag aaagccgaag atgctaaagc atcatttcta
    14221 ggccaaatgt gggctatttt taaaggtaac tggaagcaat tcttacgtgg aactggaatg
    14281 acccttctga tttccatggt cggaaccatt acaggtctct ttattggatt attaatcggt
    14341 attttccgta cagctcctaa agctaagcat aaagtagccg ccttgggaca aaaactcttt
    14401 ggttggttac tcactattta tatcgaaatc ttccgtggga cacctatgat tgttcaatct
    14461 atggttatct actacggaac agcgcaagcc tttggtattt cgatcgaccg taccctagcg
    14521 gctattttta tcgtatctat caatacgggt gcctatatga gtgaaattgt tcgcggtggt
    14581 attttcgctg tcgacaaagg tcaattcgaa gcagcaactg ctcttggctt tactcacgga
    14641 caaaccatgc gtaaaatcgt gctaccacaa gttgttcgca acattttacc agcaacaggt
    14701 aatgagtttg tcatcaatat caaagatact tctgttttga atgttatctc tgttgtggaa
    14761 ctttacttct caggtaatac cgtagccaca caaacctacc aatatttcca aacctttacg
    14821 attatcgcca ttatctactt tgttcttacc ttcacggtga cacgtattct tcgtcatatt
    14881 gaacgccgtt tcgatgccga tacttacacc actggagcaa accaaatgca gattgcggag
    14941 gtctcaaatg tctaactcaa ttattgaaat caaaaacctc aaaaaatctt acggcagcaa
    15001 tgaagtgctc aaagatatct ccctttctgt taacaaggga gaagtgattt ccattatcgg
    15061 ttcttctggt tctggaaaat caaccctcct gcgctcgatc aatcttttgg aagagccaag
    15121 tgccggtcaa atcctctttc atggtgaaga tgtccttgct gaacactaca acttgactca
    15181 ctaccgtgaa aaactaggca tggttttcca atcctttaac ttgtttgaaa acctaaatgt
    15241 attggaaaat gctatcgtcg ctcaaaccac cgtcctcaaa agagaccgtg cacaagccga
    15301 acaaatcgct aaagaaaacc tcaatgcggt tggtatgaca gaacaatact ggcaagccaa
    15361 accaaaacaa ttatcagggg ggcaaaaaca acgggttgcc attgccagag ccttatctgt
    15421 caatccagaa gccatgctct ttgatgaacc aacatcagct cttgatcctg aaatggttgg
    15481 tgaggtcctt aaaaccatgc aagacttggc aaaatcaggg ttgaccatga ttattgttac
    15541 ccatgaaatg gaatttgctc gtgatgtttc tgaccgtatc atcttcatgg ataagggatt
    15601 gattgctgaa gaaggaagtc ctcaacaaat tttcgagaat cctacacaag atcgtaccaa
    15661 agaattttta caacgcttct taaaataaga taaaggatcc aactatttct atgccagttg
    15721 ggtctttttt caattcaaaa agaatttgtc ataaaatact ttaccagtta aatattttat
    15781 gctatactta tttttgttaa cgttaataat ttatatttag gaggataata tggcagaaaa
    15841 aactgactta tcgtcagctt acagaagact gaaaagtccc aatattaaaa ccagaaaacg
    15901 tgctctgaag attattcacg agtacaaacg ttacggtaaa aaataagatt ttacttttta
    15961 tctctccaaa tttaagtcag acaaaaaaag gctaagacac cccttccatc ttagcctttt
    16021 tgtatagagt ttattaaggg aactatttta aaatatcttt tatgctagga tctagaatag
    16081 ttcaaaacgt taaaatcata catttaaggt agtgtttaaa gtatacgaaa aagaactcag
    16141 cataaaccaa gctctctttc gttataacta attatttagc aatttttgcg aagtactcaa
    16201 gagtacgtac aagttgagca gtgtaagaca tttcgttgtc ataccatgat acaactttaa
    16261 ccaattgtga tccgtcaact tccattactt tagtttgagt tgcgtcaaac aatgaaccgt
    16321 atgatacgcc tacgatatct gaagaaacga ttggatcttc agtgtaaccg aagctatcgt
    16381 ttgaagcagc tttcatagca gcgttgattt cgtcaacaga aacgtttttg tcaagagtta
    16441 caaccaactc agttactgat ccagttggaa caggaacacg ttgtgcagca ccgtcaagtt
    16501 taccgttaag ttctgggata acaagaccga tagctttagc agcaccagtt gagttaggaa
    16561 cgatatttgc agcaccagcg cgtgcacgac gaaggtcacc accacggtgt ggtccgtcaa
    16621 ggatcatttg gtcaccagtg taagcgtgga ttgtagtcat aagacctttt tgaataccga
    16681 atgcatcgtg aagagcttta gccataggag ctaaacagtt tgtagtacat gaagcacctg
    16741 agataactgt ttcagtaccg tcaagaatgt cgtggttagt gttgaaaaca actgttttaa
    16801 catcgtttcc accaggagct gtgataacaa cttttttagc accgttagca tgtaagtgtt
    16861 tttcagctgc ttctttttta gcaaagaaac cagttgcttc aagaacgatt tcaaccccat
    16921 cagttgccca gtcgatgttt tctggatcac gttcagcaga aactttgatg aagtttccgt
    16981 ttacttcaaa tccaccttct ttaacttcaa ctgttccatc aaaacgacct tgagttgtat
    17041 cgtatttcaa caagtgtgca agcatatttg gatctgtaag gtcattgata cgagttactt
    17101 caacaccttc gatgttttga atacggcgga atgcaagacg tccgatacga ccgaaaccgt
    17161 taataccaac tttaactacc attagtgatt tcctccttat gaaaatcaaa agaatattat
    17221 ttagagggaa tgctccctac ctattgtgaa aagattaact tacagtcatc tgcaacaacc
    17281 tttcaacaga actattatat aactatttca gaaaaaatgc aaataaatta gccattttca
    17341 taaagggcag cttctttcta ctagccttca aaacccacca aatataggac agggctttct
    17401 tttttctatg ttctattctg taatttatct ttttattggg aaagatttcc aaaacttcaa
    17461 actgtactat aacgttctgt tacactaaaa aagaggttag gatagttatc ctcaacctct
    17521 tagtggtggt tattcacctt tgtttttctt aatgatttct tcttgaactg acttaggaac
    17581 atcttcatag tggtcaaata ccatcatgaa tgtaccacgt ccttgagtag cagaacgaag
    17641 aactgttgca taaccgaaca tttcagcaag tggaacataa gcacgaacga tttggctgtt
    17701 accatgtgct tccataccat caacgcgtcc acgacgagct gtcacgtgtc ccataacgtc
    17761 tccaagattg tcttctggag ctgtaatcgt tacaagcatc atcggctcaa ggatagctgg
    17821 ttgtgctgat ttagctgctt ctttaagagc aagagatgct gcgatcttga aggcagtttc
    17881 agatgagtcg acatcgtggt atgaaccatc gtaaagttta gctttaacgt caaccattgg
    17941 gtaaccagca agaacaccgt ttgccataga ttcgatcaac cctttttcaa cagctgggat
    18001 aaattcacga ggaaccacac caccaacgat agcattttcg aattcgaaac ctttaccttc
    18061 ttcatttggt gtaaattcaa tccaaacatc accgaattga cctttaccac cagattggcg
    18121 tttgaagaat ccacgagctt gtgtagaagc acggaatgtt tcacggtaag atacttgagg
    18181 agcaccaacg ttagcttcaa ctttgaactc acgtttcata cggtcaacaa ggacatcaag
    18241 gtgaagttca cccataccag cgataactgt ttcaccagtt tcaacgtttg tttcaacgcg
    18301 gaatgttgga tcttcttcag caagtttttg aagggcaaca cccatcttgt cttggtctgc
    18361 tttagatttt ggctcaacca tcaactggat aactggttct ggaacttcga ttgactcaag
    18421 aataactttt gctttttcat cagtcaatga gtcaccagtt gttgtgtctt tcaaaccaac
    18481 agcggcagcg atgtcaccag cgtaaactgt ttcaatttct tgacggctgt ttgcgtgcat
    18541 ttgaaggata cgtccgatac gctcacgttt accttttgat gtgttcataa cgtaagaacc
    18601 gctattaaga acacctgagt aaacacggaa gaatgtcaaa cgacctacga atgggtcagt
    18661 catgatcttg aaggcaagag ctgcaaatgg ctcttcatca gatgctggac gttcttcttc
    18721 agcatctgta tccgggttaa cacctttgat tgcagggatg tcaagtgggc ttggaaggta
    18781 tgcgataacc gcatcaagca ttaattgaac acctttgttt ttgaaggcag aaccacaaag
    18841 tactgggaag aattcaacgt tgattgttgc tttacggata ccagcaatca attcgtcgtt
    18901 agtgatttct tcaccttcaa ggtatttcat catcaagtct tcatcagttt cagcaactgc
    18961 ttcgattaat ttttcacggt attcttgagc ttgttctaag tattcttctg gaatatcttc
    19021 ttcaaggata tctgtaccaa ggtcgttggt atagatttct gctttcatct tgatcaagtc
    19081 aatgatacca cggaaatcat cttctgcacc gattggcaat tggattgggt gtgcatttgc
    19141 ttgaagacgg tcatgaaggg tttgtactga gtaaaggaag tcagcaccga ttttatccat
    19201 tttgttggcg aaaacgatac gtggaacacc gtattcagtt gcttgacgcc aaactgtttc
    19261 agtttgaggc tcaacacctg attgtgagtc aagaacggtt acagcaccat caagaacacg
    19321 gagagaacgt tgtacttcga tggtgaagtc cacgtgtcct ggtgtgtcga tgatgttaac
    19381 gcggtgacca tcccattgag ctgttgtagc ggcagaagta atagtgatac cacgttcttg
    19441 ctcttgctcc atccagtcca tttgtgatgc gccttcgtgt gtttcaccga ttttatgaat
    19501 tttaccagtg tagtaaagga tacgctctgt tgtagttgtt ttaccagcat cgacgtgagc
    19561 catgataccg atattacgag tttttgcaag tgaaaattcg cgagccatgt gtttctccta
    19621 tttttttagt ttactctttc attatagcac tatttagcaa aaacggatag gcaggaccta
    19681 cccgttatct aaatatttgt tggtaaagat aatacctatt gcctttataa ggcttggtat
    19741 cttattcatt gaactcaggc taaacgctgt gtgaaaaaga taaactttcc tagagacaaa
    19801 ggtttcttca tcaaatttcc tattttcact ttgcgttcta tacgccttcg tatcttattg
    19861 attgagttcg agctacaaac cactgacaaa aagataatag ttcctagaaa ctgaagtttc
    19921 tgcgtcacta ttcctatttt gtcttggact tgattaacac tctcacatct tatattagtt
    19981 gaattttagc tacaaatgtc tgccaaaaag ataaaattac ctagaaactg aagtttctgc
    20041 gtcaatttcc ctattttggc tagcatttgt ttgacgctaa aatatcttat attaccaacg
    20101 gaagtgagca aaggcacggt tagcttcagc catcttgtga gtgtcttcac gtttcttaac
    20161 tgatgcacct gtgttgtttg ccgcatccat gatttcttta gcaagacgat ctttcatagt
    20221 gtgttcacca cgagctcgtg atgcgtttac caaccaacga agtccaagtg ttgtacgacg
    20281 ttctggacga acttcaactg ggacttggta gttagaacca ccgacacggc gtgcgcgtac
    20341 ttcaagtaca ggcatgatgt tgtccatagc tgtttcaaat acttcaagag cgtcatttcc
    20401 tgtcgcttct ttgatagcgc tgaaagcatc gtaaacgata gtagcagctg taccacgttt
    20461 accgtcaagc ataacacggt tgataagacg tgttacgatt tttgagttat ataatggatc
    20521 tggtaatact tcgcgtttag gcgcttgatt tttacgactc attttctctt atcccccttc
    20581 tattatcctt ttggacgttt cgcaccgtat ttagaacggc cttgtttacg gtcagcaaca
    20641 cctgcagtat caagtgcgcc acgaacgata tggtaacgta cccctggaag gtcttttaca
    20701 cgtccgccac ggataagaac aacgctgtgc tcttgcaagt tgtgtccgat acctgggatg
    20761 taggcagtta cttcgataag gttgctcaaa cgtacacgag cgaatttacg aagggctgag
    20821 ttaggttttt taggtgtcat tgttccaaca cgagtagcaa cgccacgttt ttgtggggca
    20881 gccattttag tttgaacttt tttgtgactg ttgtaaccaa tgttcaaagc tggtgaatct
    20941 gatttttcaa ttttagattt acgtggttta cgtaccaact ggttaattgt aggcatctac
    21001 attctcctgt gtttttttat ttttggttga tatagcactt ggtgacagcc catatccggt
    21061 gcactttttg caacaattgt cagcacgtct ctgtacactc ttgagagacc aaaagtaaaa
    21121 agtaccgtct aatataataa cacagacggt acttcatgtc aaatagaatt ttttctttga
    21181 tgcgattgct tataacgaat gcatcaccta gattccttac ggaacctaaa tcctgtgagg
    21241 ccgacagctg ccaacataac gactcctgct ttaagagctc tttgagtact ttcatcaaca
    21301 tcagaaatga gattgctcgt tgtagaagat cctgagccat aagatgtctt agatgagggc
    21361 tgagtaatcg aagatgtgat tttagacaca atggcattac ttgctaccat ttcagaggtt
    21421 tggccaacaa gcgcttctgc tacaagactt gtattttcag cagctaatct tgctgaagct
    21481 tctaatagtt gtttcgtttc tttaaccatt tcttgaacaa gcggagtagt atctatctta
    21541 ctatatgata gcggttttac gcccgtaaga ggtggagcta cttgcaaatc aggcacagta
    21601 gctatatctt cgtctaaatg gcgatattcc ttttcgttag ctaaggtttt aagcaaagtc
    21661 aactggtgtt ctgtggtagc aatagtagct tctagactgc tttgtttagc ttgtaaggct
    21721 gctaaagctt cttgtgcatt taacaaagat gaggtagttt tagccaaatc ttgcttagta
    21781 ttatcaatgc gctcacgtat cacttgaagg cggttaggat tcaatttaaa gtcccttaga
    21841 tattgcaaat gagctttttt agccaccagt gctgtcactc tggctgcggc ttgctctgct
    21901 aaggcttctg tctggtgcag tgcggctttc aacgatgcta gcttagccaa tgattgatca
    21961 cgagtagctt cgagttgtgc ttgttttgct ttagctgcta gtaattctgt tctcaaagaa
    22021 cctttagtat catttaattg tctcacttgg aggtttaagc tgtctgactg cttaagtgtg
    22081 cttgctaatt taccttgaag ttgacttact ttagcttggg ctgccataat atcagcttct
    22141 tgatgaatag ccgacaaacg attttctaat gagcttactt ttcctttgat cgctgcaata
    22201 gtatcagata cagtgcctac tctttgggca taatcttttg tacttccaac ggcttttata
    22261 ggggtcttat taaagcgttg atggttagca atgttagact ctggaaacat tacaaagtgt
    22321 tcattcaaag atcctacatt gctggttgaa aatccaaggt aaacaggcgc attagggtta
    22381 cgtttatcta cacgtaaaaa gttaatagca tggccgtatg tatttccgtg taaatgatct
    22441 gtaaagagca tatacttgat actgtcataa ataccacgtt taataccatt cacagtatgc
    22501 acatcgttaa aagcaccgat attctcgtac atgttatcat catttcgaat gagccctgac
    22561 gctccggcag agtcttcaat aatagtttta tcatgaggcc caacaccata atgccctgat
    22621 acccctggct gtccgtagac aaatgatggt cttgtattac catgagtttt cttatagctg
    22681 gtactaagta atcttgcaaa ttcttgtgat cctgctgtga cagtaactgg tggtagacct
    22741 aattgtctcc ttacactatt aatcatgtga gctgcaaact gcgctagctc attttgcact
    22801 tctggtgtca aattatcggg atcaacaaag cgattacgat ctgctggaat atcttggtat
    22861 tgatttaatt gattacctgg actagctttg gcaataattt gatctgcatg ctctttgtaa
    22921 taattattgt aactagctga tccaatataa ccactagctt ctaatttttt aagttcttca
    22981 agaggatagc cttgcggtgc tttcatggta ttattaccca caatgctatc ttgagtagac
    23041 ggagctgagg atttaagacg actaagttct gcctcttttt ctgctagagc agctttctga
    23101 gctgccaact cttcagtcaa ttgcttttta actttagctt tttgattttc taagtcagct
    23161 ttagccttct ccaattctga gcttaatgct tttgtattat cattagccgt ttgagctgct
    23221 ttagtgatag catcaggatt gctaatcata gcattgagct tagcaatatt ttgttcagac
    23281 gttttgactt gttccactaa atcttgagct cgagtagttt ctgctgaaat gctagctttt
    23341 tgttctgaca atgcagtctc ttttgaatgt tgatctgctt gagcattatg aagctctgtt
    23401 tcagtagctg ttaactctct ttgatgttcg gctccttggg ctaatagcgt ctcctcacta
    23461 cttgcaagag tattagtgta aatttcttgt gcagaggtta aagctttttg ttcattatct
    23521 tgctgctctt ttaagtggtt gatttcagca gtagtttttg tcagagcggt agcaagctct
    23581 gtcagttttg ttttttgttg actgagagtt ttttcaactg cttcaatagt tgcctttgct
    23641 tcttgaattg tttctggttt tggtaaacta tcgtcgtgag tattactcgc cttcgtttct
    23701 tctgaggctc tatcatctgc tttgacttga tgagatacgc ctacactggc actcaataaa
    23761 gcaattgttg aggttaaagc aattttctgc ttaacttggt ttggcttcgt ttgttctaag
    23821 tccattctct ctcctttttc catttatcga aaatattacc aacttctaca acaacattgt
    23881 tgtttttgac atcaatctct gaaactttca gcaatgattt gaaggtcatt tgctcacgct
    23941 ctgattgagc gttttctgca aacacagcaa caccaactaa agtgctgtca aattctgtca
    24001 agagactgat cattcctgta atcgtccccc cgcccttcaa gaaatcatct acaatcaata
    24061 cacgactatt gggtttcaaa ctacgctttg acagaaacat cttttcaata cgatcactag
    24121 aagcactcgc ataattgaca gacactgtcg atccttctgt tatttttaaa tcacgtctaa
    24181 caatgacaaa aggaacactt aaaatattag ccactgcatt tgctaaagga actcctttgg
    24241 ttgcaactgt catcacagcg tcaatctttt ctcctttaaa ggcattggca ataatacgac
    24301 caatattttg taaaatttta ggtgtactca gcaagtctga caaatagata tagcctccag
    24361 gtaagatgcg atcactctct gataaccttt gacacaggtc ttcaacgatg gtacgcgctt
    24421 ctgtttctga aatactgggg gtaaaaatta cgcctccact tgctcctgta agtgtatcga
    24481 tatcaccaat gttagcttct tcaaaagctt tcttgataat agcgatatct tctgagatag
    24541 aagattttgc ggcctcatat ttagttgcaa aggtgtttaa actagttaat ttatacggat
    24601 tgttaatcaa ataatttgaa atgacaacca tacgttcact tcgtcttaat ttcatagttt
    24661 ccccattttt atgtactttt tgctattata ccataaagtt aataaaaaac gaacattcga
    24721 ttaggaaaat attggttttt ttgttcttat taatatcatg caagaattca atctaagata
    24781 aactaatcaa cattcctact gaaaggacat tctacttaat aaccataaca agagccaaaa
    24841 acacttatga aaccatcata agtgttaagc acatcaaaaa tcatcacaaa aacaaaggaa
    24901 ctattacttg agaaacactg atcaatagtt tggcttgtaa aaagaccgat tatccatggc
    24961 aaaaatccga ttggtcatct cgccctcgct aacacggcta agagctgttg tcatcatcat
    25021 catattagca tcaatattgt caatcatatg aataatttca gcttccatga tgcgtggacg
    25081 taccggacta ccgtattcta attgcccgtg atggcttaaa atcacgtgcc taaggacaat
    25141 gacttcttct ttggtatcat cgatttgtag ttctgaaatt acttttgtaa tttcttcgtt
    25201 aatgagggaa atatgcccaa tcaagttacc acgaacggtg tattctgtat tatctggtcc
    25261 tgttaattct atgactttag ctaagtcatg taacataata cctgcaaaca gtagactctt
    25321 atttaagtca gggtagatat ctccaatgct atctgctagt ctgaccatcg tagcagtatg
    25381 gtaggcgagg cctgactcaa aagcatggtg gtttgtttta gctgcaggat aagtataaaa
    25441 ttctttatca tacttacgat aaagagccct tacaattcgt tgccacgttg cattttcaat
    25501 tttaaagagc atctgttcta aatagtcacg tacttcttca acactgacag gtgccttttc
    25561 tttgaagtcc tttggatcat tgggctcccc tgggcgaaca tttcgcaagg tgatttgatt
    25621 aacctgaggg gtaccattat agacctcgcg tcgccctttc ataaacacga ctttaccagc
    25681 ggtaaattct tcaacgttat aaggttgggc atcccacaga tttcctgaaa tctcgccagt
    25741 gtcatcctga aaggtaaggc taataaaatc tttaccagcc cttgtcttac gaacctctgc
    25801 cgacttaatg aggtagaacc cttcaaaaag ctggtctttt ttcatttgat taattttcat
    25861 tattttcctc ttctaataag ggcatgttca agagagattt tgttgcttgg tcttgataag
    25921 tttcaacggt attcaaggct cgaacaatgg catttgtcct tgttgaaatg agctgatcca
    25981 gcgtattatt agctgtattc atttgctttt gagccttggc aagcaggcca ccaaatttat
    26041 cgaattctaa cttgacattg cccaaaattt tactgatgtc atcagcattt ttttggatat
    26101 taagggtctt gaaaccaaca gataaggaat tgagcaaagc agacagggtc gaaggacctg
    26161 caaccacaat attttcttcc cgacgaaggc tatcaaagaa agccgcattt ctgaccactt
    26221 ctgaataaag accttctgtt ggtaagaaca taactccgaa attggtcgtc tctggggggt
    26281 tcaagtactt tttatgaatg tctttggcaa agcgtttgat agctgccaga agtgcttttc
    26341 ggctagcctc gatggccagt ttatcaccaa cttcgtaagc atcttctaat cggtaataat
    26401 cttcaagagg gaattttgag tcaatcggta ggtaaatata accgccttga ccatttcctg
    26461 ggagcttaat cgcatattct acgcgttcac tagaaccact aaccgttaca aattctcttt
    26521 cgtactggct tgatgtcatg atatcctcga tgatttggcc tagttgaagt tcgcctaaaa
    26581 tgcctcgtgt tttggtattg gacaaaacct tatttaaagt acccacatct tgagccacgc
    26641 tacgcatttc tcccaagcct ttattgacac tttctagttg cttggataca gaatcgaaag
    26701 aggcgtgcag acgatttttt aaggtttctt ctaatttttc ttcaacgatc tggcgcattt
    26761 cctccaaacg tttttcgtta gattcttgca tatttttgag cgattggttg acctgctggt
    26821 taattttttc taagcgtttg tcagaccggt ccctactatc agacaaacta cggtgcaaga
    26881 cgtcacgaat atctgttaat tgttggtaaa ggtctgcttg ttgtcggttc atcagctgtg
    26941 ttagctctaa caattgttgc ttgttagctg tatccaactg gtaggtcatt tgatcagaca
    27001 aattatccgc gttgccttct agggtttggg caagctgatg ttgaaggcca ttaactttga
    27061 acaacagata agcccctaaa cctaagagaa ccaagaccaa aaggaacaag ataaggtcca
    27121 tactaactcc aatctttact ttgaataata atcaagtagc cacttggcaa actaactgta
    27181 ataggttgat ggataaaggc attactggaa tagattttct ttttgaaaaa attatcttgc
    27241 gtcaactcaa atttagcacc agtaatggtc aagtcagctt ctccttctgc cataaatgcc
    27301 acataggtca tcccttcttc ttgatggata aggtgctgac ctgccgggcg gtaggtaatc
    27361 atattttgtt ggtcacgcaa ggcgatttgt gccataaagg gagcaatacc agggtcgcta
    27421 ggcaaaaaaa tattggataa gaggtggtcc attcgacccc caaaagcccc aaaaatggtc
    27481 acttccgcct caggaaaacg agcaaagacc tctttaaggg ctaattccgt atccgtatca
    27541 tttttttctg gattagcagt gatgagaagc tctgccttct cttttatatc cgtaaatgct
    27601 ttttgggaaa ccgaatcaaa atcaccaaca gccatattca aaggaagccc attttctaat
    27661 aaaaataggc tccctctatc aataccgaca aaataatcaa aatcacggct gatataagag
    27721 agatctcctc ccgcaaaaag agcaacttta gacattcaag gctgtcctta aggtttggac
    27781 ttggctcact aggtcactgg ccttgaaaag gtaagaacct gccacaaaga cattggcccc
    27841 tgcttcataa caagcacgaa tggtcttatt gtctactccg ccatcaacct caatgtcaaa
    27901 ggacaatcct ttttcatctc gccatttggc tacggtagcc actttttcta aacattctgg
    27961 gataaaggcc tgacccccaa aacctgggtt gactgtcata atgaggactt ggtcaactaa
    28021 gtctagcaaa ggttctaggg ctgttgcagg agttcctgga ttaatcacta cccctgcttt
    28081 catgccagct gccttgattt tttgaagggc tccatgaata tgacgagtac tttctgtgtg
    28141 aatggtcatg atatcagcac cagcttgagc aaaagcctca acatagcgtt caggatcaac
    28201 caccattaaa tgacaatcaa aaaccaattt actgtgcttt ctcatacttg ccacaacatc
    28261 tgcaccaaaa ctgatgttag gaacaaattg accatccata atgtcaatgt gaacatattc
    28321 ggcatctgtt tcttcaatac gagctagctc agaagcaaaa ttagcgtaat cagctgctag
    28381 aatagatggg gcaattttta gagttgacat ggagtagacc tactttcttt tgataacttt
    28441 tttatatgtt tcacgacggt tttcaatttc actgagaaat tgcaggtaat gctcataacg
    28501 aactggccac aactctccca tttcaagcgc tgccttgaca gcacacttgg gttcgtgggt
    28561 atgggtacag gatctaaact tacactcatg gctcagccgg cgcagctctg gaaaagcctc
    28621 gttcaaatct tcagcattag caatatcata gtctaatgat gagaaacctg gcgtatccgc
    28681 gatttttccg ccatgggtat tgtaaaaact cacagctcgg gtggtgtgac gaccacgccc
    28741 tagactatct gagatttctc ctgtttcaag agccaattcg ggggcgattc ggttgagaag
    28801 ggttgatttt ccaacacctg tttgtcccat aaagacggta attttatcag ctagcaaagg
    28861 caacaattcc tcaagactgg tcacaaaatt gtaaccgatt gcctgatact gtcgaccaat
    28921 agccttaatc tcctcaggac tatctaataa gtccatctta gagatataaa ccactggatg
    28981 aatggctttg tgttcaagca agatgagaaa gcggtctaga agattgctgt taaattctgg
    29041 ttctttagca gacataataa ccactgcttg gtcaatattg acgataggag gtcttaccaa
    29101 actgttttta cgaggatgaa tcgctaaaat ataaccttca gagttgtcct ctgctgagaa
    29161 atccacgata tctccaacat aaggtgtctc accgcgcttc ctaaaattac cacgcgctcg
    29221 cgtctgatat acctgtcctt ctgactccac gtaataaaaa ccagccagag atttaatgat
    29281 tttaccttgc aactgtgctc ctttgcccta ctataataac tctattatat cagaaaaccg
    29341 ctcccaaatc agcatttgag tctaagtttc actcgcttta aaaactgtca agattctcta
    29401 gaccactggc tatcatttag gtcactgctt tttaaaaata gaatatttta tcaactaaaa
    29461 atcagatcac tgatgatttt ttgaggtagg aaacgatgtg aattttaaaa agacctttgc
    29521 tatttacgaa aaagagtcta accgtaccgc tatagtcacg ctcttgttaa ttgccccttt
    29581 ttttccgggg tattaaaaaa actagctctg cactgcgatg ccttaacggt cagcttcaat
    29641 aagaaaaatc cccctgaact gaacatctct gttttgtcta caagggaatt ttttattgtc
    29701 accgggggaa agcttttata atccaacttc ttttaaagca tcagctaatt tgccaaaatc
    29761 ttgaatcgac aaggcctcac cacgaataga aggcttgata tctgctagtg ccagaccttt
    29821 ttcaagttta gccttgatat cttcagactt gccaaaatgg ctagtcaagt tattccacag
    29881 ggtcttgcga cgatggacaa aactgagacg agacactctg aaaaagaaat cttcgtcctc
    29941 aacctcaatc aaaggctggt cgcgacgcac cattttaagg atagcactat cgacatttgg
    30001 tgcaggtaca aagacggtgc gcggcacaat gaaggcaacc ttggcagtca tgtaatactg
    30061 caccgcaatg gacaaggaac cataagcttt agtgtttggc tcagccgaga tacgatctgc
    30121 cacttcacgc tgcatcatga ccacaaactc ttggaatgga atcttgctct caatcaagtg
    30181 catgagaata ggcgttgtga tgtagtaagg caggttagcc accaccttaa tgggcaaatc
    30241 aggattttta aactgcttga tttgggtctg caaatcagct tttaaaatat cttgattgac
    30301 cacttgcaca ttatcaaaat cacgcaaggt atctgccaaa atcggcacca agcggtcatc
    30361 aatctcaaaa gccatcactt cagcagcatt ttctgccaaa aattccgtca aagccccaat
    30421 tccaggacca atctcgataa cattgacatt ctgatcaatc tcagccgtgt caacgatttt
    30481 ctgtaaaata ttggtatcgg tcaaaaagtt ctgaccaaaa gatttcttaa aggtaaatcc
    30541 gtgacgatca aggacagtct tggtcacgct ataatctgca attctcatat attctcttct
    30601 tttattctct tctttttatg atcactttct ttttggcatt tgaaccacta gcctaaactt
    30661 ttttgccaag acacgccatt cagttaatgg ggctgttata ttttattgta ctctttactc
    30721 ctcatatgcc tccatcactt cttccacttc tgctagggta atgccaaata attcgaggcg
    30781 cttgagcagt tgtttgccat tggcatagcc gatgcggagc ttttcgccca agtattccct
    30841 gcgtttacgg ctatctgacg ccattaaaag ccctagacga atcaggtctg tttgacgaat
    30901 gtcaaaataa gactcatcat catattgctg agtgacatga gcaagtgcct tttggaggtc
    30961 ctcaaaattg gcatgttcaa cccctaagga acggccctta ctcttagaac ttggagtagc
    31021 ctcatttcgg tttaaaaagg cgtgacgagc tgttggcaca gctgccataa tgagcttacg
    31081 aatgcgctca ccattataat ctgggtctgt caaaacaatg acaccacgca agtcatttag
    31141 gcggttaatc cgttctaaat cttcttcagt gatagcagag cctctggtct cataggtatc
    31201 cacctcgtaa aaacggcgta ggttagccgt gtcgtccttg ccttctacaa caagaacctc
    31261 ttgaatatta attttttcag tcaagcttga ataccctctt tgcattagca gtcgttgctt
    31321 ttgcaacttc ttccacggtc attccacgca gctctgcgat tttatcaacc acatagcggg
    31381 tataagctgt atgattttgt ttgccacgtt ttggaactgg tgtaagataa ggggcatctg
    31441 tttccaccac aatcttatcc aaaggcaagt gctgcgctgc ctcttgaata tcaagggcct
    31501 ttttgaaagt caccacacct gaaaaagaaa tcatcatacc cagatccaca aaacgccgag
    31561 ccatctctaa tgaccctgag taagaatgca tgatgcctcc acgagggcca acaccagctg
    31621 ctttgatgat ctcataggta tcttccaagg catcgcgggt gtgcaccaca aaaggcaaat
    31681 catggtcttt tgctaattgc atttggcgct taaaaacctc tatttgcacc tctttggggt
    31741 cctccatcca ataatagtct aagccaattt cacctaaggc aatgacttta ggatgtgata
    31801 attgagagac gatagactcc tcaactgctt cagaataaga tcctgcttct gtcgggtgcc
    31861 atccaatagt agcatacacg ttaggatact ggtctgcgag agcaagtgct ccactaatag
    31921 tggcctggtc aaagccaacc acattatggt aggccacgcc catttcttga gccaaagtca
    31981 attcttcagt ttcgtgtcct tgaaattctg ctacattcag gtgcgtatgg gtatcaaaaa
    32041 tagttagttt ttctacttta tttgtcatga tgttattata acataaagat cttaaccaca
    32101 tcagaaaact tggtgagaac ttctgccgtt taggttgatc gtaaaaccct ttgctagctg
    32161 acacttgtct attttctact tttttgacaa atgaccttta atagtcggct cctacataag
    32221 ccctcaaaaa tactacaacc tactcaaata aagtgggttg aacaaatctg tatcacaaaa
    32281 agggtcgcta ccttctgtta gcgatctatg ctatactagt agatagttaa actgtaagga
    32341 gtgtgctttg ctatgcctaa aaaccaccgc ctaattacta aaatcgaagc atcattggaa
    32401 catatgacta gtttagaaaa agggattgct cattttttta ttacaaccga tctgacacct
    32461 caagagttga ccgcatccga gatagttaag cgactacaca tctctcaagc cgccttgact
    32521 cgatttgcaa aaaagtgtgg ttttactggt tatcgtgctt ttgcctttga ttaccttcac
    32581 agtcttcaag aatcacaaga gactttccaa tctattcact tagaattgac aaagcgcgtg
    32641 ctgatggact acgatgctct tattaataaa acctatgagc tcgttaatga ggaaaaatta
    32701 ctgaacttag ccaagctgat cgatgcttct gagcgagtct atttctttgg caaaggatct
    32761 tctggactag tagcaagaga aatgaagcta cgctttatgc gacttggttt aatctgtgat
    32821 gcttattcag acactgatgg ttttacttgg gcaaatagcc tcgtcaatga taattgcctt
    32881 gtttttggtt tttctttatc tggcaaaaca aactctgtca tcacagcact tcatcaagct
    32941 agtcagcgag gggccaaaac tgtcctctta acaacagata atcaaaccga atttgacgac
    33001 tccttagata ttattcccgt atcatcaacc caccaactac attatgggaa tcgtgtgtca
    33061 ccacaatttc ctcttcttat tatgatggac attatttatg cttacgtttt agcgattgac
    33121 aaaccgcaca aagaaaaaat cttcaaaaac actattattg acaaagaaaa taacgacctt
    33181 tagactacaa tagcaaaaag ggcgttgttt tctttttaaa gggtcgtcat tcacctcccc
    33241 cagtatactt aaacaagaac tcttcacgtc atatagactc acagccacta agattagcta
    33301 ctcgctaaat tattttcgat tttctcagga gttataaatg aacaggatcg ctcattcttt
    33361 tgtttaaaat ggtagtaagc tcctaatata ccagcattat ttccatgact agcaaacttc
    33421 agctgagttt tcttggcaag actagaaacc aagtagctat caagagctgt ctttaattta
    33481 tcagctaaat aatctttttg cgccatgata ccgccaccaa gtaccaccac atttgggtta
    33541 acaacgtagc agatattagc aataccttgt cctaagtaat ccacctgctt actaatggct
    33601 gctatacaaa tcgcatcacc cgctttagcc tgctcaaaaa tacgacgacc atcccattgg
    33661 ctaatgtcat ccccataagc taaggccacc tcttgaacaa gagctgtggt agatgccaga
    33721 tcctgaaact gaccatctga caaatgaagg taaccaactt cgcaagctga atgactacta
    33781 ccatgaaaaa cttgggaatt aaacaataaa cagccaccga ttcccgtccc aattgtcaag
    33841 caaagagcca caggataatc cttggcactg ccagaaatag cttcggctag accagcacag
    33901 ttaacatcgt tttccacttc acacggtaac ccgaaagtct cttcaatctc gtttttaaac
    33961 tgagttccag catagttagg aatctgaggg ccggcataaa agatttctcc cttatcagga
    34021 ttaaccattc ctgcagaaga aatggccact ccagccagat ccatttggtc ttgataggtc
    34081 tttaccaagc ccttgacctt ttccaaaata gagggaccac ctttataagc ctcagtggcc
    34141 atttcctctt tttccaacag gtctcctgtt tcagaaatca agccatactt gatagcagta
    34201 ccaccaatat caattgctaa ataatgcttc atttgttatc ccctcgcatc ttattaaaag
    34261 gtttccttag cctgattaat cagagcagca gcccgttggc aaataaccct atcttcctcg
    34321 accaactcag ctaaaggtga tcgaacagaa ccaatatcaa gaccctcatt aatacgaagt
    34381 acttccttaa taaccccata catattgccg tgagcagaaa caaggacacc gataatctca
    34441 ttaatagtat attgaaaggt cttggccttt tctaaatcct tgtcaactat aagttgattc
    34501 agccttaaaa ataattctgg catagccccg taggtaccac caataccagc agcagccccc
    34561 atcaagcgcc ctcctagaaa ttgttcatct gggccattaa aaacaatatg atcgtccccg
    34621 ccaatcgcac agaaggtttg aatatcctgc actggcatgg atgaattttt aacgccaatc
    34681 acgcgcttat tagccaacat agtcttataa aggcttgggg tcaaggcaac ccctgccagt
    34741 tgaggaatat tatagatgat aaaatctgtg tgaggagcag ccgaagaaat cgcattccaa
    34801 taatcagcaa ccgcatattc tggtaaccgg aaataaatag gcggaatagc tgcaatggca
    34861 tccacgccca aacgttctga atgagctgcc aattcaatac tatctttagt gttgttgcag
    34921 gcaacgtgat taataatcgt cagcttgccc ttagcgactg ccatgacatt ttctaacacc
    34981 aattgacggt caaaaacgct ctggtaaata cactctccag aagagccatt aatgtaaagc
    35041 ccctgaaccc ctttgtcaat atagtactgg gtcaaggcac gcacacgctc agggctaata
    35101 gctccttggt catcgtaaca agcataaaag gcaggaataa tgccttggta tttcgtaaga
    35161 tctgtcatag taatgtcctt tattcaatgt cattattaag tgggaatcgc ctaagaaggg
    35221 attccatgat aatcaagtag aggtaagtta ggctactgat gccgatgata gctagcaaag
    35281 acacgcctcc aaataacgtt aacagagaca attgtaggct aaatatcgtg agacaaataa
    35341 gagccaagaa agcaaaactc catggaaaaa agagtccggc tagtaaaaag gttcgcttaa
    35401 acagcgtaga tagggaaagc tcttttctga cggcctgagg ataagcgtac aaaaagagta
    35461 taactaccag aaataaaaca gcaatgcaaa gtactttaaa cccttgaaaa actaggcctg
    35521 attggtttcg aattaagaaa agatctaaga cacaaatact cattatcaaa agctcaataa
    35581 gacctaggcg catcccactt ttaaaataat gtttaaaatg agagctataa gtagtaacaa
    35641 gctgactaac ttgaccttct cgccaatcca acaaactcgc atacaaagcc attttagcaa
    35701 ccccaatcgt tagaagggga aggcaggaca agacaaataa gagattaaac acaagcaaat
    35761 caaacagtgc tgcactagcc ctcatccatt tactatcaag attaaaaaga gaatgtaaaa
    35821 gtccctgttt tttcgacgtc aaatcacaac ttcctttcca tcaattggtt cactgccaaa
    35881 tcaacctaat ctgccattaa cactttgatt aagcattttg taactgcttc ccctgcttgg
    35941 ttaaaattat tgggttgatg aggctcatta ggaaaaaagt aagcaaagct atcatcaacc
    36001 aaatctaggt cataggttcg atcacaggtt acaaagccaa tatcagcttc ttgttcaaaa
    36061 ggcactactt cagcttgatt acctatacca taacgaatgc actcattccc agctagcaaa
    36121 aggtggcaat ctgcataccg tttgtggtat tccaacaatg aattgtccgc tttactcaat
    36181 tgatttcttt gaataactgc aaatactttc tcccctaaaa taggatattt cccttcagct
    36241 agttgcctca aatctgtggt ttgtaaaaag gtaatcgccg catccaaatg agggtgaatc
    36301 cctcggtaac ggctaatatt ttttaaacga tcataaatca tgttagaaac ctttcttgat
    36361 ttaacctttc acagcaccca tagtaatgcc ttgggtaaat gatttttgga agacaagaaa
    36421 gaccgttaca ataggcactg cagccatggc agcacctgcc ataatcaagc cataattagt
    36481 agccatttca gcttgcatag tagcaacacc aagtgaaatc gttaaatttt cccgagaggt
    36541 taacatcacc aactgcataa aataatcatt ccaagtattg ataaaggtaa agatcgctag
    36601 cgctgcaaag cctggtttga caatagggaa agccacattg aaaaaggtgc gaatctcgcc
    36661 acatccatca atcttggcgg attccaataa ctcggttgga atattttctg agaactgttt
    36721 cataaggaaa acaccaaatg gccagcccac aagaggcaaa ataacagccg ccaaagtgtc
    36781 atggattccc ataaaattaa caatccgcac aagaggcact agaacaacct gttttggcaa
    36841 ggccatagca gcaataaaaa tcgaaaacaa aagccgctgt ccataaaacc gctttttggc
    36901 caaagcatag ccagccaaag aggaagtgcc acaaaccaag aacatggtcg ccaccgaaat
    36961 aaagacacta ttccacaacc attttaaggc tggattttgc actaccaagg ccttaaaatt
    37021 ttcaatagta ggtgcctttg gccaccattg cggtggaatc ataatggtat cggcttgagc
    37081 cttgaatgct cctgtcataa tccaataaaa tgggaaaata aacaaaatgg tcaagatgca
    37141 tagaataaca gtagtcagaa catccgatgc ggttagtttc tttttcttca tcttaatatc
    37201 ctcctaatat tccacatcat tacctaaaat tttaaattga gcaaagctga tgattgcaat
    37261 catgactgcc aagaatacac ccattgtgtt ggcatagccg tattcagaga gcttaaaggc
    37321 tttttcatac aaatagtaca tgagggtgct ggttgaataa tttggccctc cagacgtcaa
    37381 gagttgaatc aaagcaaaac attggaagga attaattgtt gttatgacag caatatataa
    37441 ggttgttggc agcagacttg gccacttgat attccaaaac acctgaaatt ccgtcgcccc
    37501 atccacacgc gcagcttcta ccaaagagtt atcaatattt cccatagctg caatatatag
    37561 aataataggc tgcccaacag atgtggttaa taaaataaca ataatagcca acaaagccca
    37621 gtgcttatct cccagccaac taatattttg ttcaatcaca tgagcatatt ttaagacata
    37681 attcaaaatc cctgacatcg gatcataaat ccatttccaa accaccgtta cggcaacact
    37741 tccggtaaca acaggtagaa agaaaactgc ccgataaaac gaacgtgcca ccacattttt
    37801 gtcataagtc ttagcagcca caaagaggga aaagaaaact acaacaggta ccgaaccaat
    37861 aacaataatc aaggtgttaa taagagactt catgaaaatg ggatcttgaa acatcctagc
    37921 ataattagca aaaccaacaa aggtaaaatc cgtcatggaa taattgaaaa aactcgtcac
    37981 aaagcccata atcatcggta tcaagacaaa aatcacaaag aaaaccaaga ccggagctaa
    38041 aaaagcgtat gagatgagcg tctctctcat ttttaattta ttgacgttca cttgtaatac
    38101 ctctttttct attaatctct cctaaaaaaa ggaaaggctc ctttccagtg accatttgac
    38161 atgtgccgtg aaaagaagcc acttgttctg aacacttatt gtgttttttt gattgtcttg
    38221 ttcgcttttt cagtgaaggc tttcaaagca tcttctggct tttcatcacc attagagacg
    38281 gcttgaacca ttgggaacca aagagttctc atttcagcaa acccatcaat cgtgttgtag
    38341 tatggtgagt agaattttgt ccattcagca atttttttca ttcgtttgtc tttgtaaaga
    38401 tccccgtaag aagttctcac aggaaaggca cctgtacgga caacattttt aggtccccat
    38461 tctttatcat cagcgataaa ttggataaat ttcttagagg cagcaacttt ttgttcatct
    38521 ttgttattaa agaccgcaaa accatttacg aggtattcta gttctggttt gccatcatct
    38581 gatgggaatg ggatttcaag gtaatccact ttactagctt ctaacaattt agcttggatg
    38641 cctggttgtg caggcgccca aagaatggta aaggaagttt ggccattcgc aaagttttga
    38701 atatcagctg aaccgtcgta ttgagagcca ttcatcatta aaccatcttt aatccaattg
    38761 gaaatcttgg tcatggcttt aatagagtta gcatcatctg tggtgtattt ggttactttg
    38821 tcatcggtaa tatgactact ataaaggtta gcaaagaagg cacgtggtcc ttggtcacca
    38881 ccttgaccat tagcaaagaa agaacctggg ttatatcctt tgtccttcaa tgctttcaag
    38941 actttttcaa aatcgtcagt tgtccaacct tctttcacca ggtcaaggac gccagcatct
    39001 ttgagcattt ttttattcaa cgccatgtag aatggtgcag agctaatagg atacatgtaa
    39061 gctgtatctc ctgcttttga ggcttgaatc aatttgtcat tgttaacatc tttggtaaac
    39121 tcttttgtga acaagtcatt caagtcagcc aatttcccat ttttaccata ttgaatgata
    39181 cgacctggag catcaaagag gacatcagga gctgttccag cttcaatagc agttgtgatt
    39241 ttctcaggac cagatgtaaa atcaatggtt tccaatttta ccttgatttc tggattagct
    39301 ttctcaaagg cagcaatcaa tttcttttcg taagttccca caccatcttc agctttttct
    39361 tgcgtaaaga ctgggaaagc ccaccatgtg atttctgttt tagcagcatc agatttactt
    39421 tcactggcct ctttctggct ctttccgcca caggctgcta atcctaaaac agatgcacca
    39481 agcatcgcta atgaagcaaa cttcttcata ttcatcgtaa aatctccttt ttttgatgtg
    39541 tgtttctatt ttgacaacca ctattaggat ttgagcgctt cgataaaacg ctctgcaatt
    39601 tccttaggcc ttgtaatggc ccctccaata acaatacctg ccacacctaa gtcattaatt
    39661 ttcttggctt cttctgggga atgaatcttt ccttcggcaa tgacagcaat gcctgctttg
    39721 caaagagctt ctatcaatgc cacatcagga cctgcttctt gacggctata tggggtgtag
    39781 cctgagaggg tcgttccaac aaaatcaatg ccagcctgat gggccactag tccttcatca
    39841 aaggtactaa tatctgccat caaaagctga ttggggtact tttccttaac ttgtcgaata
    39901 aagctagcaa tatccaagcc atcatgtcgg tcacgcttgg tacaatccat agcaatcact
    39961 gcaatattta aggcagctaa ttggtccact tcggtcatcg ttgccgtgat aaaaggctct
    40021 tgcggcgggt aatctttttt gatgataccg ataattggta ggtctgttat ggcttgaatt
    40081 tctttaatat ccctaaccga attagctctg atgccgacag caccagcttc ttgagcagct
    40141 tttgccagca gtggcataat acccccagtc tctgaataaa gaggctcacc tggcaaagcc
    40201 tgacaagaaa cgataatctc accctttaac tgctccatga gcttttcttt ggttggttta
    40261 tca
//
LOCUS       DAUCLR010000006        39076 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.006, whole genome shotgun sequence.
ACCESSION   DAUCLR010000006 DAUCLR010000000
VERSION     DAUCLR010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39076
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.006"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            65..1057
                     /gene="manX"
                     /locus_tag="VGT87_000226"
     CDS             65..1057
                     /gene="manX"
                     /locus_tag="VGT87_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEQ3813118.1"
                     /translation="MGIGIIIASHGKFAEGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYGHFNNAIQQFDADDEILVLADLWSGSPFNQASRVAGENPDRKMAIITGLNLPMLIQ
                     AYTERLMDAGAGVEQVAANIIKESKDGIKALPEDLNPVEETAATEKVVNALQGAIPAG
                     TVIGDGKLKINLARVDTRLLHGQVATAWTPASKADRIIVASDEVAQDDLRKQLIKQAA
                     PGGVKANVVPISKLIEASKDPRFGNTHALILFQTPQDALRAVEGGVEINELNVGSMAH
                     STGKTMVNNVLSMDKEDVATFEKLRDLGVTFDVRKVPNDSKKNLFELIQKANIK"
     gene            1146..1955
                     /locus_tag="VGT87_000227"
     CDS             1146..1955
                     /locus_tag="VGT87_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEQ3813119.1"
                     /translation="MSDISIISAILVVIIAFFAGLEGILDQFQMHQPLVACTLIGLVT
                     GHLEAGVILGGTLQMLALGWANIGAAVAPDAALASVAAAIIMVKSGDFTQKGITFAYS
                     TAIPLAVAGLFLTMIVRTLSTALVHAGDKAAAEGNFAGIERFHFIALLLQGLRIAVPA
                     ALLVAVPTSAVQSVLNAMPNWLNEGMQIGGAMVVAVGYAMVINMMATREVWPFFALGF
                     ALAAISQLTLIAMGVIGVAIAFIYLNLSKKGGNGGNAAGSADPIGDILEDY"
     gene            1972..2883
                     /locus_tag="VGT87_000228"
     CDS             1972..2883
                     /locus_tag="VGT87_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEQ3813120.1"
                     /translation="MTEQIKLTKSDRQRVWWRSQFLQGSWNYERMQNMGWAYALIPAL
                     KKLYTSPEDRAAALERHMEFFNTHPYVAAPIIGVTLALEEERANGTPIDDKAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGALGASLASTGNIVGPLLFFFGWNLIRMAFLWYTQE
                     FGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVSINFTIDLPGKQLSDGA
                     YVVFPDGAVKGAELKTILANAIGGMSLDKVQAQTLQGQLDSLIPGLAGLLLTFLCMWL
                     LKKKVSPIAIIIGLFAFGILAHLAGIM"
     gene            2997..3356
                     /locus_tag="VGT87_000229"
     CDS             2997..3356
                     /locus_tag="VGT87_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEQ3813121.1"
                     /translation="MAQSLNTSVEYKTKAVSYLGMGGKVGHILLGDKALEFYNDKNVN
                     DYIQIPWTTINHIGANVSRKKVSRHFEIFTDQGKFLFASGDSGKILKITRQHIGNEKV
                     ITLPTLMQTFINKFFRH"
     gene            3748..5025
                     /gene="serS"
                     /locus_tag="VGT87_000230"
     CDS             3748..5025
                     /gene="serS"
                     /locus_tag="VGT87_000230"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEQ3813122.1"
                     /translation="MLDLKRIRTDFDTVAAKLKNRGVSEDTLTHLKELDEKRRALLVQ
                     SEELKAERNIASAAIAQAKRQKEDATQQIADMQKVSADIKTIDNQLVAIDQQVTDIIT
                     VLPNTPHDSVPVGADEEDNVEIRRWGTPRDFDFEVKAHWDLGEDLDILDWERGAKVTG
                     ARFLFYKNLGARLERALYNFMLDEHIKEGYQEIITPYMVNHDSMFGTGQYPKFKEDTF
                     ELADTNFVLIPTAEVPLTNYYRGEILDGKELPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYQELEKMTANAENILQKLGLPYRVISLCTGDMGFSAAKTY
                     DLEVWIPAQNTYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEVLRPYMGGETVISPK"
     gene            complement(5258..6028)
                     /locus_tag="VGT87_000231"
     CDS             complement(5258..6028)
                     /locus_tag="VGT87_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEQ3813123.1"
                     /translation="MTDVSRVLKEARDQGRLTTLDYANLIFDDFMELHGDRHFSDDGA
                     IVGGLAYLAGQPVTVIGIQKGKNLQDNLARNFGQPNPEGYRKALRLMKQAEKFGRPVV
                     TFINTAGAYPGVGAEERGQGEAIAKNLMEMSDLKVPIIAIIIGEGGSGGALALAVADQ
                     VWMLENTMYAVLSPEGFASILWKDGSRATEAAELMKITAGELYQMGIVDRIIPEHGYF
                     SSEIVDIIKANLIEQITSLQAKPLDQLLDERYQRFRKY"
     gene            complement(6025..6891)
                     /locus_tag="VGT87_000232"
     CDS             complement(6025..6891)
                     /locus_tag="VGT87_000232"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEQ3813124.1"
                     /translation="MALFRKKDKYIRITPNNFLKGSVSHNVPEVPDELFAKCPACKHM
                     IYKKDLGLAKICPTCSYNFRISAQERLTLTVDEGSFQELFTSIETKDPLRFPGYQEKL
                     QKAKETTGLHEAVLTGKAMVKGQQIALAIMDSHFIMASMGTVVGEKITRLFELAIEEN
                     LPVVIFTASGGARMQEGIMSLMQMAKVSAAVKRHSNAGLFYLTILTDPTTGGVTASFA
                     MEGDIILAEPQSLVGFAGRRVIETTVRENLPDDFQKAEFLQDHGFVDAIVKRTELRDK
                     IAHLVAFHGGGQ"
     gene            complement(6900..8264)
                     /locus_tag="VGT87_000233"
     CDS             complement(6900..8264)
                     /locus_tag="VGT87_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEQ3813125.1"
                     /translation="MFKKILIANRGEIAVRIIRAARELGISTVAVYSEADKEALHTIL
                     ADEAICIGPARSKESYLNMNSVLSAAIVTGAQAIHPGFGFLSENSKFATMCEEMNIKF
                     IGPSASVMDKMGDKINARSEMIKAGVPVIPGSDGEVYNAQEALAIANKIGYPVMLKAS
                     AGGGGKGIRKVETEADLEAAFNAASQEALGAFGNGAMYLEKVIYPARHIEVQILGDAY
                     GNIIHLGERDCSLQRNNQKVLEESPSIAIGNTLRHEMGQAAVRAAEAVAYENAGTIEF
                     LLDEDSEKFYFMEMNTRIQVEHPVTEFVTGVDIVKEQIKIAAGQPLAINQEDITITGH
                     SIECRINAENTAFNFAPSPGKITDLYMPSGGVGLRVDSAVYNGYAIPPYYDSMIAKII
                     VHGSNRFDALMKMQRALVELEIEGIITNTDFQLDLISDKRVIAGDYDTSFLMETFLPH
                     YNKD"
     gene            complement(8296..8718)
                     /gene="fabZ"
                     /locus_tag="VGT87_000234"
     CDS             complement(8296..8718)
                     /gene="fabZ"
                     /locus_tag="VGT87_000234"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEQ3813126.1"
                     /translation="MMDIREIQAALPHRYPMLLVDRVLEVSDDHIVAIKNVTINEPFF
                     NGHFPHYPVMPGVLIMEALAQTAGVLELSKEENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFIKRRGTIAVVEARAEVDGKLAASGTLTFACGQ"
     gene            complement(8715..9215)
                     /locus_tag="VGT87_000235"
     CDS             complement(8715..9215)
                     /locus_tag="VGT87_000235"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEQ3813127.1"
                     /translation="MNIQEIKDLMAQFDTSSLREFLFKTNEGELIFSKNEQHLNASTS
                     NQEHAVPVPQVQLVPNPTASEASSPASVKDVPVEEQPQAESFVAEGDIVESPLVGVAY
                     LAASPDKPPFVAVGDTVKKGQTLVIIEAMKVMNEVPAPCDGVITEILVSNEDVIEFGQ
                     GLVRIK"
     gene            complement(9217..10449)
                     /gene="fabF"
                     /locus_tag="VGT87_000236"
     CDS             complement(9217..10449)
                     /gene="fabF"
                     /locus_tag="VGT87_000236"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEQ3813128.1"
                     /translation="MTFKRVVVTGYGLTSPIGHDPETFWNNLKAGQIGIGPITKFDTT
                     DYAVKNAAEIQDFPFDKYFVKKDLNRFDRYSLYALYAAKEAINHADLNIEMIDSDRFG
                     VIVASGIGGIAEIEEQVIRLHEKGPKRVKPMTLPKALPNMAAGNVAMSLKAQGVCKSI
                     NTACASSNDAIGDAFRAIKFGTQDVMIVGGSEAAITKFAIAGFQSLTALSTTEDPSRS
                     SIPFDKDRNGFIMGEGSGMLVLESLEHAQERGATILAEIVGYGNTCDAYHMTSPNPEG
                     LGARKAIHLALQEAGIEASAINYVNAHGTSTPANEKGESQAIVAVLGKDVPVSSTKSF
                     TGHLLGAAGAIEAIATIEAMRHNYVPMTAGTQALSEDIEANVIFGEGKETAINYAISN
                     TFGFGGHNAVLAFKCWEE"
     gene            complement(10464..11198)
                     /gene="fabG"
                     /locus_tag="VGT87_000237"
     CDS             complement(10464..11198)
                     /gene="fabG"
                     /locus_tag="VGT87_000237"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEQ3813129.1"
                     /translation="MEIKGKNIFITGSTRGIGLAMAHQFASLGANIVLNGRSAISEEL
                     VASFTDYGVTVVTISGDVSEASEAKRMVNEAIESLGSIDVLVNNAGITNDKLMLKMTE
                     EDFERVLKINLTGAFNMTQSVLKPMIKARQGAIINVSSVVGLTGNIGQANYAASKAGM
                     IGFTKSVAREVAARNICVNAIAPGFIESDMTGVLPEKMQEQILSQIPMKRIGKAQEVA
                     HLASFLVEQDYITGQVIAIDGGMTMQ"
     gene            complement(11188..12126)
                     /gene="fabD"
                     /locus_tag="VGT87_000238"
     CDS             complement(11188..12126)
                     /gene="fabD"
                     /locus_tag="VGT87_000238"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEQ3813130.1"
                     /translation="MTKTAFLFAGQGAQKLGMARDFYDNFAIVRKTFDQASQVLGYDL
                     RRLIDSDELKLNQTSYTQPAILTSSIAIYRVLGLHHVKPDMVAGLSLGEYSALVASGA
                     LSFEDTLSLVAKRGRLMEEAAPQGSGKMVAVMNTDVQVIEEVCQIAAKHGVVAPANYN
                     TPSQIVIGGQTDAVNVAVELLKERGVKRLIPLNVSGPFHTALLEPASRLLAKELERYN
                     FSDFKIPLVGNTEANIMEKDRIPELLARQVMEPVRFYDSVATLVESGITQFIEVGPGK
                     VLTGFVKKIDKNLLCTSVENMVSLRLFLDREDIHGN"
     gene            complement(12151..13122)
                     /gene="fabK"
                     /locus_tag="VGT87_000239"
     CDS             complement(12151..13122)
                     /gene="fabK"
                     /locus_tag="VGT87_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEQ3813131.1"
                     /translation="MKTRITELLNIDYPIFQGGMAWVADGDLAGAVSNAGGLGIIGGG
                     NAPKEVVKANIDRVKAITDRPFGVNIMLLSPFADDIVDLVIEEGVKVVTTGAGNPGKY
                     MERLHQAGIIVVPVVPSVALAKRMEKLGVDAVIAEGMEAGGHIGKLTTMSLVRQVVEA
                     VSIPVIAAGGIADGHGAAAAFMLGAEAVQIGTRFVVAKESNAHQNFKDKILAAKDIDT
                     VISAQVVGHPVRSIKNKLTSAYAKAEKAFLIGQKTATDIEEMGAGSLRHAVIEGDVVN
                     GSVMAGQIAGLVRKEESCETILKDIYYGAARVIQNEAQRWQSVSIEK"
     gene            complement(13323..13547)
                     /locus_tag="VGT87_000240"
     CDS             complement(13323..13547)
                     /locus_tag="VGT87_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEQ3813132.1"
                     /translation="MAVFEKVQEIIVEELGKETEEVTLETTFDDLDADSLDVFQVISE
                     IEDAFDIQIETEEGLNTVGDLVAYVEEKSK"
     gene            complement(13608..14582)
                     /locus_tag="VGT87_000241"
     CDS             complement(13608..14582)
                     /locus_tag="VGT87_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEQ3813133.1"
                     /translation="MIFSKISQVAHYVPQQLVTNNDLASIMDTSHEWIFSRTGIAERH
                     ISRDEMTSDLAIQVADQLLTQSGLKADAIDFIIVATISPDATMPSTAAKVQAAIAATS
                     AFAFDMTAACSGFVFALAMADKLIASGAYQNGMVIGAETLSKLVNWQDRATAVLFGDG
                     AGGVLLEASKDKHVLAETLHTDGARCQSLISGETSLSSPYSIGKKAIATIQMDGRAIF
                     DFAIRDVSKSILTLMAQSDITKDDIDYCLLHQANRRILDKIARKIDVPREKFLENMMR
                     YGNTSAASIPILLSEAVQKGQIRLDGTQKILLSGFGGGLTWGSLIVKI"
     gene            complement(14583..15017)
                     /locus_tag="VGT87_000242"
     CDS             complement(14583..15017)
                     /locus_tag="VGT87_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEQ3813134.1"
                     /translation="MEYDKINQYLVDIFNRILVIEEMSLKTSQFSDVSLKEMHTIEII
                     GKYDQVTPSDIARELMVTLGTVTTSLNKLEAKGYIARTRSRSDRRVVYLSLTKRGRLL
                     DRLHAKFHKNMVGHVIADMSDEEMQALVRGLGNLHQFLEDLV"
     gene            complement(15094..15885)
                     /locus_tag="VGT87_000243"
     CDS             complement(15094..15885)
                     /locus_tag="VGT87_000243"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEQ3813135.1"
                     /translation="MQFKHIIFDVVDDLATLTLNRPEVSNGFNIPICQEILVALAEVK
                     RDTSVRFLLIKAVGKVFSVGGDLVEMQEAVAKDNVQSLVKIAELVQEISFAIKHLPKP
                     VILCADGAVAGAAFNIALAVDFCIASTQTKFIQAFVNVGLAPDAGGLFLLTRAVGLNR
                     ATHLVMTGEGITADKGLDYGFVYRTAESDKLDKVCLQLLKRLRRGSSNSYAGMKSLVW
                     QSFFTGWEDYAKAELAIQEELAFKEDFKEGVIAFGERRRPNFQGK"
     gene            complement(16105..17241)
                     /gene="dnaJ"
                     /locus_tag="VGT87_000244"
     CDS             complement(16105..17241)
                     /gene="dnaJ"
                     /locus_tag="VGT87_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEQ3813136.1"
                     /translation="MNNTEYYDRLGVSKDASQDDIKKAYRKMSKKYHPDINKEAGAEQ
                     KYKDVQEAYETLSDSQKRAAYDQYGAAGAQGGFGGGAGGFGGFDGGGFGGFEDIFSSF
                     FGGGGSRNPNAPRQGDDLQYRVNLSFEEAVFGVEKEVSYNREATCGTCLGSGAKPGTA
                     PVTCRKCHGSGVMTIDTQTPLGMMRRQVTCDICHGSGKEIKEPCQTCHGTGHEKQAHK
                     VSVKIPAGVETGQQIRLQGQGEAGFNGGPYGDLFVILNVLPSKQFERNGSTIYYNLDI
                     SFTQAALGDTVEIPTVHGDVEMAIPAGTQTGKTFRLKGKGAPKLRGGGQGDQHVTVNI
                     VTPTKLNDAQREALQAFAEASGEKMLHPKKKGFFDKVKDALEDI"
     gene            complement(17522..19357)
                     /gene="dnaK"
                     /locus_tag="VGT87_000245"
     CDS             complement(17522..19357)
                     /gene="dnaK"
                     /locus_tag="VGT87_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEQ3813137.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPETIISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGMDKTDK
                     DEKILVFDLGGGTFDVSILELGDGVFDVLATAGDNKLGGDDFDQKIIDFLVAEFKKEN
                     GIDLSQDKMALQRLKDAAEKAKKDLSGVTQTQISLPFITAGSAGPLHLEMSLSRAKFD
                     DLTRDLVERTKTPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQHIVIKSNDGLSEEEIDRMMKDAEANAEADAKRKEEVDLKNE
                     VDQAIFATEKTIKETEGKGFDTERDAAQSALDELKAAQESGNLDDMKAKLEALNEKAQ
                     ALAVKMYEQAAAAQQAAQAAQGAEGAQANDSANNDDVVDGEFTEK"
     gene            complement(19538..20110)
                     /gene="grpE"
                     /locus_tag="VGT87_000246"
     CDS             complement(19538..20110)
                     /gene="grpE"
                     /locus_tag="VGT87_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEQ3813138.1"
                     /translation="MAVFNKLFKRRHSVSEEIKKDDLQEEVEATETEETVEEVIEETP
                     EKSELELANERADEFENKYLRAHAEMQNIQRRSSEERQQLQRYRSQDLAKAILPSLDN
                     LERALAVEGLTDDVKKGLEMTRDSLIQALKEEGVEEVEVDSFDHNFHMAVQTLPADDE
                     HPADSIAEVFQKGYKLHERLLRPAMVVVYN"
     gene            complement(20113..21147)
                     /gene="hrcA"
                     /locus_tag="VGT87_000247"
     CDS             complement(20113..21147)
                     /gene="hrcA"
                     /locus_tag="VGT87_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001872861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEQ3813139.1"
                     /translation="MITQRQNDILNLIVELFTQTHEPVGSKALQRTIDSSSATIRNDM
                     AKLEKLGLLEKAHTSSGRMPSPAGFKYFVEHSLRLDSIDEQDIYHVIKAFDFEAFKLE
                     DMLQKASHILAEMTGYTSVILDVEPARQRLTGFDVVQLSNHDALAVMTLDESKPVTVQ
                     FAIPRNFLTRDLIAFKAIVEERLLDNSVIDIHYKLRTEIPQIVQKYFVTTDNVLQLFD
                     YVFSELFLETVFVAGKVNSLTYSDLSTYQFLDNEQQVAISLRQSLKEGEMASVQVADS
                     QEAALADVSVLTHKFLIPYRGFGLLSLIGPIDMDYRRSVSLVNIIGKVLAAKLGDYYR
                     YLNSNHYEVH"
     gene            complement(21282..21851)
                     /locus_tag="VGT87_000248"
     CDS             complement(21282..21851)
                     /locus_tag="VGT87_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEQ3813140.1"
                     /translation="MRKRLKFPYFLTLLACFLLLIVCPLLSSQRIASADKEVRVNYSQ
                     KQFITKMGKEVKPLAKYYGIRPSILIAQILLETHDGKTLLASKYHNLFSKKATPGQAA
                     ITLKSPKQTNQNVRYAIYKDDASAIRDYLRMLRQGKEVDKRLYRNLATEKGYKAPAKS
                     LQKYLHYTDKTYARRLIQVIESNDLTNYD"
     gene            complement(21848..22591)
                     /locus_tag="VGT87_000249"
     CDS             complement(21848..22591)
                     /locus_tag="VGT87_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEQ3813141.1"
                     /translation="MKYNSLNYLVQLLIVIVFLGGLYLFIKPEESVTPTQLNKKEIQQ
                     KDIKKTDRLRALPKVSVEDWELVLVNRDHITKEMSPELADINGISVDKRIEQATSDFL
                     AAAQAIDPQEHLISGYRSVAYQAELYQSYIKKEMANDPTLTQESAEALVQTYLQPPGA
                     SEHHTGLAIDISTVDTLNASDPSVSKAVQKIAPDYGFVLRFPEGKKTSTGVDYEDWHY
                     RYVGKASARYMAQHNLTLEEYIAALKEKR"
     gene            complement(22581..23288)
                     /locus_tag="VGT87_000250"
     CDS             complement(22581..23288)
                     /locus_tag="VGT87_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEQ3813142.1"
                     /translation="MTKTRLYIARHGKTMFNTIGRAQGWSDTPLTKKGEEGIRELGLG
                     LKDATIPFKAAFSSDSGRTMQTIEIILRESENEFLPYTKDNRIREWCFGSLEGTYDSE
                     LFLGVLPRTKAFENRDNLRDVPYSELAESIVEIDTANWAEPWEVLRKRIWEGFEAIAL
                     SIQNAGGGNALVVSHGMTIGTFLWLIDPDRDKQYIDNGSVTVVEFDDGQFTIKTIGDM
                     SYRYRGREIIEERSDEI"
     gene            complement(23680..23919)
                     /locus_tag="VGT87_000251"
     CDS             complement(23680..23919)
                     /locus_tag="VGT87_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEQ3813143.1"
                     /translation="MIWSLIVGAFIGMIAGKMTEKSQSMGCFTNIIAGLIGSAVGQRL
                     FGDWGIQLAGMAIFPSILGAAIVIVLVSAIFGKRY"
     gene            complement(24134..24349)
                     /locus_tag="VGT87_000252"
     CDS             complement(24134..24349)
                     /locus_tag="VGT87_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813144.1"
                     /translation="MIVADSNKLKYFVSEKKAVGLSQAAFLYVMLVTAYKKTIIYAKI
                     RLTGIALEPSGVSDGYLMNHHVCLHYY"
     gene            complement(24346..25785)
                     /gene="gatB"
                     /locus_tag="VGT87_000253"
     CDS             complement(24346..25785)
                     /gene="gatB"
                     /locus_tag="VGT87_000253"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEQ3813145.1"
                     /translation="MNFETIIGLEVHVELNTNSKIFSPSSAHFGEDPNANTNVIDWSF
                     PGVLPVMNKGVIDAGIKAALALNMDIHKEMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEIKLEDGSTKKIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEIIQYTGISDVKMEEGSMRVDANISLRPYGQEQFGTKTELKNLNS
                     FSNVRKGLEFEVERQAKLLRSGGVIRQETRRYDEANKGTILMRVKEGAADYRYFPEPD
                     LPLYEIDDAWIDEMRAQLPQFPAQRRAKYEEELGLSTYDASQLTATKALSDFFETAVS
                     LGGDAKQVSNWLQGEVAQFLNAEGKTIEEIALTPENLVEMIAIIADGTISSKMAKKVF
                     VHLAKNGGSARAYVEKAGLVQISDPAVLVPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVAQQLLAQELQKLLD"
     gene            complement(25785..27251)
                     /gene="gatA"
                     /locus_tag="VGT87_000254"
     CDS             complement(25785..27251)
                     /gene="gatA"
                     /locus_tag="VGT87_000254"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEQ3813146.1"
                     /translation="MSFNHKTIEELHDLLVAKEISATELTQATLEDIKSREEAVGSFI
                     TVSEEVALKQAAAIDAKGIDADNLMSGIPLAVKDNISTKEILTTAASKMLYNYEPIFN
                     ATSVANAYAKDMIVIGKTNMDEFAMGGSTETSYFKKTKNAWDHTKVPGGSSGGSATAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGVVGLKPTYGTVSRYGLIAFGSSLDQIGPFAPTV
                     KENAQLLNVIASSDVKDATSAPVRIADYTSKIGRDIKGMKIALPKEYLGEGIDPEIKE
                     TVLAAAKQFEALGATVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGFRADDA
                     KNLDEIYVNTRSQGFGDEVKRRIMLGTFSLSSGYYDAYFKKAGQVRTLIIQDFDKVFA
                     DYDLILGPTTPTVAFGLDTLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFVDGLPVG
                     LQLIGPKYAEETIYQAAAAFEAVTDYHKQQPIIFGGDK"
     gene            complement(27251..27553)
                     /gene="gatC"
                     /locus_tag="VGT87_000255"
     CDS             complement(27251..27553)
                     /gene="gatC"
                     /locus_tag="VGT87_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008086915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEQ3813147.1"
                     /translation="MKISEEEVRHVAKLSKLSFSESETTTFATTLSKIVDMVELLNEV
                     DTEGVAITTTMADKKNVMRQDVAEEGTDRALLFKNVPEKENHFIKVPAILDDGGDA"
     gene            complement(27785..28033)
                     /locus_tag="VGT87_000256"
     CDS             complement(27785..28033)
                     /locus_tag="VGT87_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEQ3813148.1"
                     /translation="MIRSRSRPLIRKGLAVSLARSVKPELKLGICGEHGGDPASIAFC
                     HSQGLTYVSCSPFRVPLTRLAAAQAAIKASGHSLTQDK"
     gene            complement(28548..29102)
                     /locus_tag="VGT87_000257"
     CDS             complement(28548..29102)
                     /locus_tag="VGT87_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEQ3813149.1"
                     /translation="MRALISIDYTNDFVADDGKLSAGKSAQAIATKIAEVTKTAFDQG
                     DYIFFAIDCHDQNDPWHPESKLFAAHNIKGTTGRHLYGPLAEVYSHMKQHPRVFWIDK
                     RYYSAFSGTDLDIRLRERGITQLVLTGVLSDICVLHTAIDAYHLGYQLEIVKSAVASL
                     TKESYEWSLAHFEQVLGAKLINDY"
     gene            complement(29249..30031)
                     /gene="codY"
                     /locus_tag="VGT87_000258"
     CDS             complement(29249..30031)
                     /gene="codY"
                     /locus_tag="VGT87_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEQ3813150.1"
                     /translation="MPNLLEKTRKITSILQRSVDSLETELPYNTMASRLADIIDCNAC
                     IINGGGTLLGYAMKYKTNTDRVEEFFEAKQFPDTYVKAASRVYDTEANLSVENELTIF
                     PVESKDTYPGGLTTIAPIYGGGMRLGSLIIWRNDNEFSDDDLILVEISSTVVGIQLLN
                     LQTENLEDTIRKQTAVNMAINTLSYSEMKAVAAILGELDGNEGRLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVINEGIFAKLKEF"
     gene            complement(30249..31463)
                     /locus_tag="VGT87_000259"
     CDS             complement(30249..31463)
                     /locus_tag="VGT87_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEQ3813151.1"
                     /translation="MKIIEKSSKLEHVAYDIRGPVLDEANRMIASGEKILRLNTGNPA
                     AFGFEAPDEVIRDLIVNARLSEGYSDSKGIFSARKAIMQYCQLKGFPDVDIEDIYLGN
                     GVSELISMSLQALLDNGDEVLVPMPDYPLWTACVSLGGGKAVHYLCDEEAGWYPDIAD
                     IKSKITSRTKAIVVINPNNPTGALYPKEILEDIVALAREHQLIIFADEIYDRLVMDGK
                     EHIAIASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKDHVKGYIEGLNMLANMRL
                     CSNVLAQQVVQTSLGGHQSVDALLLPGGRIFEQRNFIYEAINAIPGLSAVKPEAGLYL
                     FPKIDRQMYRIDDDEEFVLQLLKQEKVMLVHGRGFNWKDPDHFRIVYLPSVEELEDVQ
                     EKITRVLYQYRR"
     gene            31694..32146
                     /locus_tag="VGT87_000260"
     CDS             31694..32146
                     /locus_tag="VGT87_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015055992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEQ3813152.1"
                     /translation="MSLKYKRILVAIDGSYESELAFNKGVNVALRNDATLLLVHVIDT
                     RALQSVATFDTYIYEKLEQEAKDVLDDFEKQAQIAGITNIKQIIEFGNPKNLLAHDIP
                     DRENADLIMVGATGLNTFERLLIGSSSEYIMRHAKIDLLVVRDSTKTL"
     gene            complement(32268..33656)
                     /locus_tag="VGT87_000261"
     CDS             complement(32268..33656)
                     /locus_tag="VGT87_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEQ3813153.1"
                     /translation="MTVKAVFFDIDGTLLNDRKNIQKTTQKAIQQLKKQGIMVGLATG
                     RGPGFVQPFLENFGLDFAVTYNGQYILTRDKVLYQNQLPKSMIYKVIRYANEKKREIS
                     LGTASGLAGSRIIDMGTSPFGQVISSFVPKSWARTVEGSFKHLIRRIKPQSFRNLVTI
                     MREPIYQVVLVASQAETKKIQEKFPHIKITRSSPYSLDLISVDQSKIKGIERLGEMFG
                     FDLSEVMAFGDSDNDLEMLSGVGIGVAMGNAETVVKDGAHFTTDSNNNDGISKALAHY
                     GLIHFDVEKSFKSRDENFNKVKDFHRLMDSDTIETPRSYTISEAGYRSGFKVEEIVEF
                     LYAASKGDQQQFTQAIFDLHGAVDQAANKVQAKKHVETPLIGQVDALADLLYFTYGSF
                     VLMGVDPQPIFEAVHEANMAKIFPDGKAHFDPVTHKIQKPDYWQERHAPEVAIKKELD
                     KQLQKSLQRFDK"
     gene            33728..34693
                     /locus_tag="VGT87_000262"
     CDS             33728..34693
                     /locus_tag="VGT87_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEQ3813154.1"
                     /translation="MKKILVLHTGGTISMQADNSGRVVPNQDNPMTKIHAAAQDIQLT
                     VSDFLNLPSPHITPHHMLSIYHHIQERTDVFDGIVITHGTDTLEETAYFLDTMALPTN
                     IPVVLTGAMRSSNEVGSDGIYNYLTALRVASSDKAKEKGVLVVMNDEIHAAKYVTKTH
                     TTNISTFQTPTHGPLGIIMKNDLLFFKTAEPRIRFNLRCISGTIPIIKAYAGMGDGSI
                     LSLLTPGSIQGLVIEALGAGNVPPLAVGEIEHLITLGIPVVLVSRCFNGMAEPVYAYE
                     GGGAMLQEAGVMFVKELNAPKARLKLLIALNAGLTGQELKDYIEG"
     gene            complement(35042..35746)
                     /locus_tag="VGT87_000263"
     CDS             complement(35042..35746)
                     /locus_tag="VGT87_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ3813155.1"
                     /translation="MISYLKADLFRIRQERLALFSCMLLVVIAVGVAYSFRKTPNQVI
                     MIQIINIITDFVTLFFLTPARIFFSDDYNYRTINNVVVKQQNRTLIIIYKLLVSFGLS
                     LLFISLTYLLSALAFHLFTGQAYYGIVIDRMIKQLPFYIAIIALCQFLFNILRKTYQS
                     ITAYILYVLLFDKVSATLANHFFKLDISPVLMFKHLSLSLGDKHLSSTSILVAFGLTL
                     CYFLGNVFLFSKRELK"
     gene            complement(35759..36661)
                     /locus_tag="VGT87_000264"
     CDS             complement(35759..36661)
                     /locus_tag="VGT87_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ3813156.1"
                     /translation="MTQQSMLLSIEQVNKSYGKNQVLSDISFDIYKGEICGLVGQNGA
                     GKTTLMRILSGLIGKDSGQIKQLQPYRMGSIIESPTLYPNMTAHDNLYYAALQLRLAD
                     AKERIHEVLELIGLEKVSKKKKVKDYSLGMRQRLAIGLSILDFPEFLILDEPINGLDP
                     AGIKEMRQIILNLRDCYGITILISSHILSELDLVVDRYVIMHKGKVIKSMDKAELKAQ
                     VKVQIALHTSDDQLVKNRLLELGLRVETDGQMLLIKPTLSVMELIKIVLDLPVEIFDI
                     YHHQVSFEHYYLDLLGKDAASPLI"
     gene            complement(36954..38969)
                     /gene="recG"
                     /locus_tag="VGT87_000265"
     CDS             complement(36954..38969)
                     /gene="recG"
                     /locus_tag="VGT87_000265"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEQ3813157.1"
                     /translation="MILTAPMSNLKGFGPKSAEKFQKLDIYTVEDLLLYYPFRYEDFK
                     SKSVFDLVDGEKAVITGLVVTPANVQYYGFKRNRLSFKLRQGEAVLNVSFFNQPYLAD
                     KIELGQEVAVFGKWDATKSAITGMKVLAQVEDDMQPVYRVAQGISQSTLIKAIKSAFE
                     IDAHLELKENLPATLLEKYRLMGRSQACLAMHFPKDITEYKQALRRIKFEELFYFQMN
                     LQVLKAENKSETNGLPILYSKHAMETKISSLPFILTNAQKRSLDDILSDMSSGAHMNR
                     LLQGDVGSGKTVIAGLSMYAAYTAGFQSALMVPTEILAEQHYISLQELFPDLSIAILT
                     SGMKAAVKRTVLAAIANGSVDMIVGTHALIQDSVQYHKLGLVITDEQHRFGVKQRRIF
                     REKGENPDVLMMTATPIPRTLAITAFGEMDVSVIDELPAGRKPIMTRWVKHEQLGTVL
                     EWVKGELQKDAQVYVISPLIEESEALDLKNAVALHAELSTYFEGIAKVALVHGRMKND
                     EKDAIMQDFKDKKSHILVSTTVIEVGVNVPNATIMIIMDADRFGLSQLHQLRGRVGRG
                     HKQSYAVLVANPKTDSGKKRMTIMTETTDGFVLAESDLKMRGSGEIFGTRQSGIPEFQ
                     VADIVEDYPILEEARKVSAAIVSDPNWIYEKQWQLVAKNIRKKEVYD"
BASE COUNT        11712 a         8450 c         6833 g        12081 t
ORIGIN      
        1 gttttggaag gagattattc ttcttcattt caacgaaaaa tattttaaag gaggaagaac
       61 aagaatgggt atcggtatta ttattgccag ccatggtaaa tttgctgaag gtattcatca
      121 atctggttct atgatttttg gtgaacaaga gaaagttcaa gttgttactt tcatgccaaa
      181 cgaaggacca gatgatttgt atggacactt caacaatgct atccaacaat ttgatgctga
      241 tgatgaaatc cttgtgctag ctgacctttg gagtggttct ccatttaacc aagctagtcg
      301 tgtggcaggt gaaaatcctg accgcaaaat ggcgatcatc actggtctca acttgcctat
      361 gcttattcaa gcttacactg aacgtcttat ggatgctggt gcaggtgttg aacaagttgc
      421 tgcaaacatc attaaggaat ctaaggacgg tatcaaagcc cttcctgaag accttaaccc
      481 agttgaagaa actgctgcaa ctgaaaaagt tgttaacgca cttcaaggag ctattcctgc
      541 tggaactgtc atcggagatg gtaaattaaa gatcaatctt gctcgtgttg acactcgtct
      601 tttacacggt caagttgcaa cagcatggac tccagcttct aaggctgacc gtatcattgt
      661 tgcttctgat gaggttgctc aagatgacct tcgcaaacaa ttgatcaaac aggctgcacc
      721 tggtggtgtc aaagctaacg ttgttccaat tagcaaattg attgaagctt ctaaagatcc
      781 tcgttttggt aacacacacg cacttattct tttccaaacc cctcaagatg cccttcgcgc
      841 ggttgaaggt ggtgttgaaa tcaacgaatt gaacgttggt tcaatggctc actcaactgg
      901 taaaacaatg gttaacaacg ttttatcaat ggacaaagaa gacgttgcta cttttgaaaa
      961 attacgcgac ctaggtgtta cattcgacgt ccgtaaagtt cctaacgatt ctaagaaaaa
     1021 cttatttgag cttatccaaa aagctaacat taaataagtt tcaaacgcta taaggtttta
     1081 aaacgatgac tagctaactt attagcaaaa agtcctcgtt tactatactt tgaaaggatt
     1141 acaacatgtc agatatttcg attatttctg caattttggt cgttatcatt gccttcttcg
     1201 ctggtcttga aggtatcctc gaccaatttc aaatgcacca accacttgtt gcctgtactt
     1261 tgattggact tgtaactgga caccttgaag ctggtgtcat ccttggtggt acacttcaaa
     1321 tgcttgctct tggttgggca aacatcggtg ctgccgttgc ccctgatgct gcccttgcct
     1381 ctgttgcagc tgctatcatc atggtcaaaa gtggcgactt tactcaaaaa ggaattacct
     1441 ttgcttactc aacagctatc ccacttgctg ttgctggtct tttccttaca atgatcgttc
     1501 gtactttgtc aactgccctt gtacacgctg gtgacaaagc tgccgctgaa ggtaatttcg
     1561 caggtattga acgcttccac tttattgccc ttcttcttca aggattgcgt atcgctgtcc
     1621 cagcagctct tcttgttgct gttcctactt cagccgtaca atctgttttg aatgctatgc
     1681 caaactggtt gaacgaaggt atgcaaatcg gtggtgcgat ggttgttgct gtaggttatg
     1741 ctatggttat caacatgatg gctactcgtg aagtatggcc attcttcgcc ctaggttttg
     1801 ctcttgcagc tatcagccaa ttaaccctta tcgctatggg tgttatcggt gtagcaattg
     1861 ccttcatcta ccttaatctt tctaaaaagg ggggtaacgg tggtaatgca gcaggttcag
     1921 ctgatccaat cggcgacatc ttagaagact actagaaagg ggtaagaaca aatgactgaa
     1981 caaattaaat taactaaatc agaccgtcaa cgtgtttggt ggcgttcaca attcttacaa
     2041 ggttcttgga actacgaacg tatgcaaaac atgggctggg cttatgcact tatccctgct
     2101 cttaaaaaat tgtatacgtc tcctgaagac cgtgcagctg ctcttgagcg ccacatggaa
     2161 ttctttaaca ctcacccata cgttgcagct ccaatcattg gtgtaacact tgcccttgaa
     2221 gaagaacgcg caaatggtac gccaattgat gacaaggcta tccaaggggt taaaatcggt
     2281 atgatgggac ctcttgctgg tatcggtgac ccagtattct ggtttacggt tcgtcctatc
     2341 cttggggcac ttggtgcttc tttggcttca acaggtaaca tcgttggtcc acttctgttc
     2401 ttctttggat ggaaccttat ccgtatggcc ttcttatggt atactcaaga atttggttac
     2461 aaagctggtt ctgaaattac taaagacatg tcaggcggta tcctccaaga cattactaaa
     2521 ggtgcttcaa tccttggaat gtttatcctt gcagtgcttg ttcaacgttg ggtatctatc
     2581 aactttacta tcgaccttcc tggaaaacaa ttgtcagatg gtgcctatgt ggtattccca
     2641 gatggcgctg ttaaaggtgc tgagttgaaa actatccttg ccaacgctat tggtggtatg
     2701 agccttgata aagtccaagc tcaaaccctt caaggacaat tggactctct tatcccaggt
     2761 ttagctggat tactccttac attcctttgc atgtggttat tgaagaaaaa agtttctcca
     2821 atcgcaatca tcatcggctt gtttgcattt ggtatccttg ctcaccttgc aggtattatg
     2881 taatcaacca atggaagtct gagatgaact caggcttctt ttttatgctt tatgtcagga
     2941 aaatcccact taaaatatgg tataataact acaactacgc aatgaaagga acttaaatgg
     3001 ctcaatcttt aaacactagt gtcgaatata agacaaaagc tgtctcctac cttggaatgg
     3061 gaggcaaagt tggtcatatt ttactcggtg acaaggccct tgaattttac aacgataaaa
     3121 atgtgaatga ttatattcaa attccttgga caactatcaa tcacatcggg gctaatgttt
     3181 caagaaaaaa agttagccgc cactttgaaa tttttacgga ccaaggcaag tttttatttg
     3241 cttctggaga ctctggaaaa atcttaaaaa tcactcgtca acatattggt aatgagaaag
     3301 tgattacatt accaaccttg atgcaaacct ttattaacaa attttttcga cattaaaaaa
     3361 ataatgcgca gtgccgattt atcatttcaa gaaagcaaag aatagtagct ctaaattgct
     3421 atttagtgga aaagattccc tcatttgagt ttactttttc ttgttttttg ttataatagt
     3481 cctaacggat aagtaatacc tttttttcat tcagagagtc tgtggatgct gtgaacagat
     3541 agggaaaatg atatgaaatt ctaccgtgtc atcaataaaa tagagattaa gtgcacagat
     3601 tgtgaagctg gatggaaccg cgccaaagcg ctccagcatc ttcataagct gtgctttttc
     3661 tatcgtcaga gaatcacaag atccaactgt cttacttagc cttgtttcac tgacggctac
     3721 taataactat cttaaaggag cttctgtatg ctagatctta aacgtatccg taccgatttt
     3781 gacactgtcg ctgccaaatt aaaaaaccgc ggtgtttctg aagacaccct gacccatctt
     3841 aaagaacttg atgaaaaacg tcgtgctcta ttagtgcaat cagaagaatt aaaagcagag
     3901 cgtaatattg cttctgctgc catcgctcaa gccaaacgtc aaaaagaaga tgccactcaa
     3961 caaattgctg atatgcaaaa agtctctgca gatatcaaaa ctattgacaa tcaactagta
     4021 gctatcgacc agcaagttac tgacatcatc actgttttac ctaatactcc tcacgattct
     4081 gtccctgttg gagctgacga agaagacaat gtcgaaattc gccgttgggg aacgcctcgt
     4141 gactttgact tcgaagttaa ggctcattgg gatctaggcg aagatcttga tattttagac
     4201 tgggaacgtg gagcaaaggt aactggtgct cgtttcttat tctataaaaa tcttggtgct
     4261 cgtttggagc gtgctcttta taacttcatg ctggatgaac atatcaaaga aggttatcaa
     4321 gaaatcatca caccttacat ggttaaccac gattccatgt ttggtacagg acaatatcca
     4381 aaattcaaag aagacacctt tgaattagca gataccaact ttgtcctgat tccaactgct
     4441 gaagtgcctt tgaccaatta ttaccgtggg gaaatcctag acggcaaaga attgccaatt
     4501 tactttactg caatgagccc atcttttcgt tctgaagcgg gttctgcggg tcgtgacaca
     4561 cgtggcctta tccgcctaca ccaattccac aaagttgaaa tggttaaatt tgctaaacca
     4621 gaagaatctt accaagaact agaaaaaatg actgctaatg ctgaaaatat tcttcaaaaa
     4681 cttggtttgc catatcgcgt catttccctt tgtactggag acatgggctt ctcagctgct
     4741 aaaacctatg accttgaagt ctggattcct gcacaaaaca cctaccgtga aatttcatca
     4801 tgttcaaata cagaagattt ccaagcacgt cgtgctcaaa tccgttaccg cgatgaggct
     4861 gacggtaagg tcaaactcct tcacaccctt aatgggtcag gacttgctgt cggtcgtacc
     4921 gtagcagcca ttttagaaaa ctaccaaaat gaagatggtt ctgttaccat ccctgaagta
     4981 ctccgccctt acatgggtgg cgaaaccgtc atttcaccta aataataaac tcaccccaga
     5041 gatcattaaa gtgatttctg gggtttacta ttttaaagag ccaccacatc acttgaaact
     5101 cactacattg cccctttaac aacacttttc ttcacactag tttatcaaaa gtcttattag
     5161 tatacccatt tccttattta caagactagt taaaaaccct tttggaaagg cctcccttag
     5221 cttgataaac acgtagggag actccaaaag gatgttgtta atatttacga aagcgttggt
     5281 agcgctcatc taataattgg tctaatggct tagcttgcaa actggttatt tgttcgatga
     5341 ggttagcttt gatgatgtca acgatttcac ttgaaaaata accatgttct gggataatac
     5401 gatctactat gcccatttga tacaattcgc ctgcggtaat tttcatcaat tcagcagcct
     5461 ctgtcgctct tgaaccatct ttccataaaa tagaagcaaa accttctgga ctaaggactg
     5521 catacatggt attttcaagc atccagacct gatcggcaac cgctaaggct aatgcaccac
     5581 cagagcctcc ttcaccaata atgatagcga taatgggaac cttgagatca ctcatttcca
     5641 tcaaattttt agcaatggcc tcaccctgtc ctcgttcttc cgcaccgaca cctggatagg
     5701 ctcctgcagt attgataaag gtgacaacgg gacgtccaaa tttttctgcc tgtttcataa
     5761 ggcgcaaagc tttacgataa ccttctggat tgggctggcc aaaattcctt gccaaattat
     5821 cctgtaaatt cttacctttt tgaataccaa tgaccgtaac aggttgtccc gccaaataag
     5881 ctaaaccacc tacaatggca ccatcatctg aaaaatggcg atcgccatgc agttccataa
     5941 agtcatcgaa aataaggttg gcgtaatcca aagttgttaa acgcccttga tcacgcgctt
     6001 cttttaaaac tcttgataca tctgtcattg gccacctcca tgaaatgcta ctaagtgggc
     6061 aatcttgtct cgcaactctg ttcgtttcac aatagcatcc acaaaaccat ggtcttgtaa
     6121 aaattctgct ttttggaaat catctggcaa gttttcacga actgtggtct caatgacacg
     6181 acgacctgca aagccaacca aggattgagg ttctgccaaa ataatatccc cttccatggc
     6241 aaaactagct gttaccccac cagtagttgg atctgtcaaa atagtgaggt aaaagaggcc
     6301 tgcattagaa tgtcttttca cagctgcaga gaccttggcc atttgcatga ggctcatgat
     6361 accctcttgc atacgggcgc ctcctgaagc cgtaaaaatc accaccggca gattttcttc
     6421 aatagccaat tcaaacaatc gggtaatttt ttccccaaca acagttccca tactagccat
     6481 aataaagtga gaatccataa tagcaagagc aatttgttgt cctttaacca tagctttccc
     6541 tgtcaaaaca gcttcatgca gacctgtcgt ttccttggct ttttgtaatt tttcctgata
     6601 acctggaaaa cgtaacggat ccttggtttc aatacttgta aataattcct ggaaagagcc
     6661 ttcatcaact gttaaggtca gccgctcttg ggcagaaatc ctaaaattgt aagaacaggt
     6721 tgggcaaatc tttgccaatc ccaaatcttt cttataaatc atgtgcttac aagctgggca
     6781 tttcgcaaag agttcatctg gaacttctgg gacattatgg ctgacagatc ctttgaggaa
     6841 attattggga gtaatacgga tgtatttatc ttttttacga aataaagcca tattttctcc
     6901 taatctttat tgtaatgtgg caggaacgtt tccatcaaga aagaggtatc ataatctcct
     6961 gcaatcactc gcttatctga aatcaaatcg agctggaagt ctgtattggt gataatgcct
     7021 tcaatttcca attcaactaa ggcacgttgc attttcatca aagcgtcaaa tcgattgctt
     7081 ccatgaacga tgattttagc aatcatgctg tcataataag gaggaatggc atagccattg
     7141 taaactgctg aatcaactcg tagtcctaca ccaccacttg gcatatacaa gtctgtaatc
     7201 tttcctggac tcggtgcaaa gttaaaagca gtattttcag cattgatacg acattcaata
     7261 gaatgtcccg tgatagtgat gtcttcttga ttaatcgcca aaggctgacc tgctgcaatt
     7321 ttgatttgct ctttgacaat atccactcct gtcacaaatt cggtgacagg atgctcaact
     7381 tggatacggg tattcatttc catgaaatag aatttctcgc tgtcttcgtc caacaaaaac
     7441 tcaatggttc ccgcattttc ataggctaca gcttcagctg ctctaaccgc tgcttgcccc
     7501 atttcgtgtc tgagggtatt gccaatagca atggatggac tttcttccaa gactttttga
     7561 ttattccgtt gcaaggagca atcccgttct cccaagtgaa tgatgttacc gtaagcgtct
     7621 cctaaaattt gcacctcaat atgtctagct ggataaatta ctttttccaa atacatggcg
     7681 ccatttccga aagctcccaa agcttcttga ctggcagcat tgaaagccgc ctccaaatct
     7741 gcttctgtct ccactttacg aatcccttta cctccaccac ctgccgatgc ttttagcatg
     7801 acaggataac caatcttatt ggcaatagct aaggcttctt gagcgttata aacttcacca
     7861 tctgaccctg gaataacggg cactcctgcc ttaatcattt cagaacgagc attgatttta
     7921 tcacccattt tatccataac cgatgcggat ggaccgataa atttaatatt catctcttca
     7981 cacatggtcg caaactttga gttttcactt aaaaagccaa accctgggtg aatcgcttgc
     8041 gctcctgtca caatagctgc tgacaagacg gaattcatat taagatagga ttccttggaa
     8101 cgggcaggac caatacagat agcctcgtcc gccaatatcg tgtgcagagc ttccttatcc
     8161 gcttccgaat aaactgcaac tgtggaaatc cctaattctc gtgcagcacg aatgatacgc
     8221 actgcaattt caccacgatt agcaattaag atttttttaa acataagggt ttcttcctcc
     8281 gcagaacaat cattctcatt gcccacaggc aaaggttaat gttccacttg ctgctagctt
     8341 gccatcaact tctgccctgg cttcaacgac agcaatggta ccgcgacgct tgataaaggt
     8401 tgctgtcatc acaagttgat caccagggac gacttgtttt ttgaacttca ctttatccat
     8461 tccagcgtaa aagaccaact tgcctttatt ttcttctttt gacaattcta agacaccagc
     8521 tgtttgagcc aaagcttcca taatcagaac acctggcatc acaggatagt gaggaaaatg
     8581 cccgttaaag aaaggttcat taatagtcac atttttaatg gcaacgatat ggtcgtctga
     8641 cacttctaat acacgatcaa ccaataacat tgggtagcga tgaggcaaag ccgcttgaat
     8701 ttctctaata tccatcattt gatccgcacc aatccttgtc caaattcaat gacatcttca
     8761 tttgatacca aaatttctgt aatcacacca tcacatggcg ctggcacttc gttcatcacc
     8821 ttcatggctt cgataatcac aagggtttgt ccttttttta cagtatctcc gactgccaca
     8881 aatggtggct tgtcagggct agctgcaaga taggctacac cgactaaagg actttcaaca
     8941 atatcacctt ctgctacaaa agattctgct tgtggttgct cctctacagg aacatcctta
     9001 actgatgctg gactagacgc ttccgaagca gttggattcg gaacaagttg tacctgtggt
     9061 acaggcacag catgttcttg atttgatgtc gatgcattga ggtgttgttc gtttttacta
     9121 aagattagct caccttcatt tgttttgaat aaaaattcac gtaggcttga ggtatcaaac
     9181 tgagccatca aatctttgat ttcttgaata ttcaacctac tcctcccaac atttgaaagc
     9241 taatacagca ttatgaccac caaatccaaa agtatttgaa atggcatagt tgatagctgt
     9301 ttcttttcct tctccaaaga tgacatttgc ttcgatatcc tctgataaag cttgtgtccc
     9361 agccgtcatc ggaacatagt tatgacgcat agcctcgata gtggcaatag cttcaatagc
     9421 gcctgctgct ccaagaaggt gacccgtaaa cgattttgtt gaagacaccg gaacatcctt
     9481 accaagaacg gctacaattg cttgactttc tcctttttca ttagctgggg ttgaagttcc
     9541 gtgcgcattg acataattaa tagcactggc ttcaatacca gcttcttgta aggctaagtg
     9601 gatggcttta cgagctccta atccctcagg atttggagat gtcatatggt aggcatcgca
     9661 ggtatttcca taacccacaa tttctgctaa gattgtcgcc ccgcgttctt gcgcatgttc
     9721 taggctttct aaaacaagca ttcctgatcc ttctcccatg ataaaaccat tgcggtcttt
     9781 atcaaatgga atagagcttc tgcttggatc ttcagttgtt gagagagctg ttaaactttg
     9841 gaagccagca atggcaaact tggttatggc cgcctctgac ccaccaacta tcatcacatc
     9901 ttgagtccca aatttaatgg cacgaaaggc atccccaatc gcatcatttg aagaagcgca
     9961 ggcagtatta attgatttac atactccttg agcttttaaa gacatggcga catttcctgc
    10021 agccatattt ggtagcgctt ttggcaacgt cattggcttc acacgttttg gccctttttc
    10081 atgtaagcga atcacttgct cttcaatctc cgcaattcca ccaatacctg aagcaacaat
    10141 aacgccaaat cgatcggaat caatcatctc aatatttaaa tcagcatgat taatagcctc
    10201 tttcgctgcg taaagagcat aaagagaata tctatcgaaa cggttcaaat cttttttcac
    10261 aaagtatttg tcaaatggaa aatcctgaat ttcagcagca ttcttgactg cataatcagt
    10321 cgtgtcaaat ttagtaatag ggccaatacc aatttggcca gctttcagat tgttccaaaa
    10381 cgtttcagga tcatgcccga ttggagatgt tagaccataa cctgttacca ctactctttt
    10441 aaatgtcatt tcactacgtc tccttattgc attgtcatac cgccatcaat agcgataact
    10501 tgtcccgtaa tataatcttg ttctaccaag aagcttgcta agtgagcaac ttcttgagct
    10561 tttccaatac gtttcattgg gatttgactt aagatttgct cttgcatttt ttcaggaagg
    10621 acacctgtca tgtcggactc gataaatcct ggagcaatag cattcacgca aatgttacga
    10681 gcagcaactt cacgggcaac agacttggta aaaccaatca ttcctgcctt agaagcggca
    10741 tagtttgctt gcccaatatt gcctgttaaa ccaaccacac tagagacatt aataattgct
    10801 ccttggcggg ccttgatcat tggttttaac actgattgag tcatgttaaa agcaccggtc
    10861 aaattgattt tcaagacgcg ctcaaaatcc tcttccgtca ttttaagcat caatttatca
    10921 ttagtaatgc cagcattgtt aactaaaaca tcaatcgaac ctaaactttc gatagcttca
    10981 ttgaccatac gtttagcttc tgatgcttct gaaacatccc cagagatggt aacaactgta
    11041 acaccgtagt cagtaaaact agcaacaagc tcctcagaga tagcagagcg accatttaag
    11101 acaatattag ctcctaaact ggcgaattga tgagccattg ctaaaccgat acctctcgtt
    11161 gagcctgtga taaatatatt ttttccttta atttccatga atgtcctctc tatctaaaaa
    11221 taaacgtaag cttaccatgt tttcaacact agtacatagt aaatttttat caattttctt
    11281 cacaaaacct gtcaaaacct tacctggtcc tacctcaatg aattgtgtta tgccactttc
    11341 tactaaagtc gcaacactgt cataaaaacg aacaggctcc atgacttgac gggctaatag
    11401 ttctgggata cggtcttttt ccataatatt agcttcggta ttaccaacca aaggaatctt
    11461 gaagtcactg aagttgtatc tttccaactc tttagccaac aagcggctag ctggttctaa
    11521 caaagcagtg tggaaaggac ctgacacgtt taaagggatt aaacgcttaa ctcccctttc
    11581 ctttaaaagt tcaactgcca cgttcacagc atctgtctga ccaccaatta caatttgact
    11641 aggagtatta tagtttgctg gagcaaccac tccatgttta gcagctattt gacagacttc
    11701 ttcgatgact tgcacgtctg tattcataac ggcaaccatc tttccagatc cttgtggtgc
    11761 tgcttcctcc atcaagcgcc ctctcttagc tactaaggat agggtatctt caaaagagag
    11821 tgcccctgat gctaccaaag ctgagtactc tcctaaggaa agaccagcta ccatatccgg
    11881 tttaacgtga tgtaagccca aaacacggta aatagcaatt gatgatgtca aaatagctgg
    11941 ttgcgtatag ctagtttgat taagttttaa ctcgtcacta tcaatcaaac ggcgcaaatc
    12001 gtatcccaat acttgactag cttgatcaaa ggtttttctt acaatagcaa agttatcata
    12061 aaaatccctt gccatcccta atttttgggc accttgaccg gcaaataaaa aggctgtctt
    12121 tgtcattatc attatccttc atgtgtttat ctacttttct attgaaacag attgccagcg
    12181 ctgagcttca ttttgaataa cacgagctgc accataataa atatctttta aaatcgtttc
    12241 acagctttct tcttttctca caagccctgc aatttggcca gccataacag atccattgac
    12301 tacatcgcct tcaataacag catgtcgaag cgatcctgct cccatttctt caatatcagt
    12361 agctgttttt tgaccaatta aaaatgcttt ttctgcttta gcgtaagctg aggtcaattt
    12421 atttttaata gaacggacag ggtggcccac aacctgcgca gaaatcaccg tatcaatatc
    12481 ttttgctgct aagattttat ctttaaaatt ttggtgggca ttggattctt tagcaacaac
    12541 aaagcgagtt ccaatttgaa cagcctctgc tcctaacata aatgctgctg ctgcaccatg
    12601 accatcagct ataccacctg ccgcaatgac aggaatcgaa accgcttcaa caacttgtct
    12661 tactaaagac atagtcgtta acttgccaat atgtcctcca gcttccatac cctcagcaat
    12721 cactgcatct accccaagct tttccatacg tttggctagc gcaacgcttg ggacaacagg
    12781 aacaacgatt atacccgcct ggtgcagtct ttccatatac tttcctggat ttcctgcgcc
    12841 tgttgttact actttaacac cttcttcaat gaccagatca acgatatcat cagcaaaagg
    12901 agataaaagc atgatattaa ccccaaaagg tctatcagta atagctttaa cacggtcaat
    12961 attggcttta acaacttctt ttggagcatt tcccccaccg atgataccta gcccaccagc
    13021 attggacact gctcctgcta aatcaccatc cgcgacccag gccatacctc cttgaaaaat
    13081 gggataatca atattaagta attctgtaat acgtgttttc ataataactt tctataaaaa
    13141 ataaggagaa taatttgata atcaaactat tatagtaaga agaaagagcg agctagatcc
    13201 taaaaagagt ctaatcccac cctaatggtt tgattaatac ttattgtttt tttagtgatg
    13261 aacatgaaca taacagcagt aagcttttca tagacttctg atgttacttg tcacttttat
    13321 ccttattttg acttttcctc aacgtaagca acaaggtcac caacagtatt taatccttct
    13381 tctgtttcaa tttgaatatc aaaagcatct tcaatttctg aaataacttg gaaaacatca
    13441 agtgaatctg catctaaatc atcaaatgta gtttctagtg ttacttcttc tgtctcctta
    13501 ccaagttctt caacaataat ttcttgtact ttttcaaata ctgccatgtt tatttctcct
    13561 taaaaaatat aaatttgttt ttataaatgt gctaagcaca tgaataacta gattttaaca
    13621 attagacttc cccatgtcaa acctccacca aaaccggaaa gtaagatttt ttgggtacca
    13681 tctaacctaa tctgtccctt ttgaacagcc tctgacaaga ggattgggat actagcagca
    13741 ctagtattac cataacgcat catgttttca aggaattttt cccgaggaac atcaatcttg
    13801 cgtgctattt tatcaagtat acgacgatta gcttgatgca ataaacaata gtctatatca
    13861 tcttttgtaa tatctgattg cgccattagt gtgaggatgc tttttgacac atccctaatc
    13921 gcaaaatcaa atatggcacg tccatccatc tgaatagtcg ctatggcttt tttgccaatt
    13981 gagtaaggag atgaaagact agtttctcct gaaattaaac tctgacatct cgcaccatct
    14041 gtgtgtaatg tttccgctaa aacatgttta tctttactgg cttccagtag cacgccacca
    14101 gctccgtctc caaaaagaac ggctgtcgct cgatcctgcc aatttactaa cttagataaa
    14161 gtctctgcac ctattaccat tccgttttgg taggcacctg atgcaataag cttatctgcc
    14221 atagccaagg caaagacaaa gccactacaa gcagcagtca tatcaaaagc aaaagcactt
    14281 gtagccgcaa tagctgcttg aactttcgca gctgttgatg gcatagtggc atcagggctg
    14341 atagttgcaa caataataaa atcaatagca tcagctttta acccagactg agtcaaaagc
    14401 tgatcagcaa cctgaatagc taggtcacta gtcatttcat ctctagaaat atggcgttct
    14461 gctattcctg ttcgagaaaa gatccactca tgactagtgt ccattatgct agctaaatca
    14521 ttattagtca ctaactgctg tggaacatag tgggctacct gactgatttt agaaaaaatc
    14581 atttaaacca aatcctccaa aaattgatgt agattcccta gtccacgcac caatgcctgc
    14641 atctcttcat cactcatatc agcaatgaca tgtccaacca tatttttatg gaactttgca
    14701 tggaggcggt ccaaaagtcg accccttttt gttaatgaca ggtaaacaac tcgcctatct
    14761 gagcgtgatc gcgttcttgc gatataacct tttgcttcta atttatttaa gctagtcgtt
    14821 actgtcccta acgttaccat taattctctg gcgatatcac ttggagtcac ctgatcatat
    14881 ttgccgataa tttcaatagt gtgcatctct tttaaagaga catcactaaa ctgactcgtc
    14941 tttaagctca tttcttcaat aaccaaaatc cggttgaaaa tatcaactaa gtattggtta
    15001 attttgtcat attccaagta aacacctcct agcaataaac tttgacaatc aaattatatc
    15061 aaagatcaaa gtattttgca agcaatagct gtcttactta ccttgaaaat ttggtcggcg
    15121 cctttctcca aaagctatga caccttcttt aaaatcttcc ttgaaagcta gctcctcttg
    15181 aatagcaagc tctgccttag cataatcttc ccatcctgtg aaaaagcttt gccagaccaa
    15241 tgatttcatt ccagcataag aatttgaaga gccacgtctc aatcgcttta aaagttgaag
    15301 acaaactttg tctagtttat cagattctgc cgtacggtag acaaacccat aatcgagacc
    15361 tttatcagca gtaattcctt cacctgtcat cacaaggtga gttgcacgat taagtcctac
    15421 tgcacgagtc aatagaaata aaccaccggc atccggtgct agtccaacat ttacaaaagc
    15481 ttggataaat tttgtttgag tactagcgat acaaaaatca acagctaaag ctatattaaa
    15541 agccgctcca gctacagctc catcggcgca aaggatgact ggttttggca aatgcttgat
    15601 tgcaaaagaa atttcttgta ccagttcagc aatcttgact agcgactgca cattatcttt
    15661 tgcaacagct tcttgcattt caactaggtc accaccaact gaaaacactt ttccaacggc
    15721 tttaattaat aaaaaacgca cagatgtatc gcgtttaact tctgctaatg ctaccagaat
    15781 ctcttgacaa attggaatat taaaaccgtt tgaaacctct ggacggttta aagtcaatgt
    15841 cgcaagatca tctacaacat caaagatgat gtgtttaaac tgcataacaa tcctcttttc
    15901 tgataattta tttgactaag aaattatttc tctttaaaat catttgataa tcaaatcaat
    15961 tacctaatta tgatatacta aataaaccat tttgacaaga gaaaaaaacg atatctataa
    16021 aagaaaacgt tttctctaac tgttttgacg taaaaaaacg tataacttcg ggaaaattat
    16081 cctgaaatta tacgtttgac atctttaaat atcctctaag gcatctttca ctttatcaaa
    16141 aaaacctttt ttctttggat gtaacatttt ttcaccactc gcttctgcaa atgcttgcaa
    16201 ggcttctctt tgagcgtcat tgagtttggt tggggtcaca atattaacag taacatgttg
    16261 atccccttga cctccaccac ggagttttgg tgctcctttg cctttgaggc ggaaggtttt
    16321 acctgtttgt gtcccagcag ggatagccat ctcaacatcg ccatgaaccg ttggaatttc
    16381 aacagtatct cctaaggctg cttgagtaaa agaaatatct aagttgtagt aaatagtgga
    16441 accattgcgt tcaaattgct tacttggtaa gacattcaaa ataacaaata aatcaccata
    16501 aggtccgcca ttaaagccag cttctccttg accttgtaaa cggatttgct gaccagtctc
    16561 tacaccagct ggaatcttaa cagacacttt atgagcttgc ttttcatgac cagtaccgtg
    16621 acaggtttga cagggttctt taatttcctt accactacca tgacaaatat cgcaggtgac
    16681 ttgtcgacgc atcataccaa gtggtgtttg ggtgtcaatt gtcattacac ctgaaccatg
    16741 gcacttacga caagttactg gagcggtacc aggttttgca cctgagccta aacaagtgcc
    16801 gcaggttgct tcgcgattat agctaacttc tttttcaaca ccaaatacag cctcttcaaa
    16861 agataaatta acacgatatt gcaaatcgtc accttgacga ggggcatttg ggtttcggct
    16921 tccaccgcca ccaaagaagc ttgagaagat atcttcaaaa ccaccaaagc cacctccgtc
    16981 aaaaccacca aagccgcccg ctccgccacc aaagccgcct tgagcacctg cagcgccgta
    17041 ttgatcataa gcagcacgct tttgtgaatc acttaaggtt tcgtaagctt cttgaacatc
    17101 cttatacttt tgctccgctc cagcttcttt attgatatct ggatgatatt tcttagacat
    17161 ttttcgataa gcttttttga tatcgtcctg agaagcgtcc ttagaaaccc ccagacgatc
    17221 gtaatattct gtattgttca taatgtaaga taccaagccg taaaacgact caatgaaaaa
    17281 taggaaatcc gacaaagata ctctcgtatc taggaggtat ttctcttttt tcgcagagtc
    17341 gtaggcgtgt tcgaatccgc aaaagagtct ttacacgctt tagcctgtgt tcgaataatc
    17401 gaacttgtat tcctttctta aaatattttg acgtcagcat ttttacattt tatttagcca
    17461 aaaacagagg tttggtacaa cctctgaatt cggatattaa tgttactaga taactaaatc
    17521 attacttttc tgtaaattcg ccatctacaa catcatcatt atttgctgaa tcattagctt
    17581 gtgcaccttc tgcaccttgt gctgcttgtg ctgcttgttg agctgctgca gcttgctcgt
    17641 acattttaac agccaaagct tgcgcttttt catttaatgc ttcaagttta gctttcatgt
    17701 cgtcaaggtt gccagattct tgcgcagctt ttaactcgtc aagagctgat tgcgctgcat
    17761 cgcgttctgt atcaaagcct ttaccttcag tttctttgat tgttttttca gtagcaaaga
    17821 tagcttggtc aacttcgttt ttaaggtcaa cttcttcttt acgtttcgca tcggcttcgg
    17881 cattagcttc agcgtctttc atcatgcgat caatttcttc ttcagaaagt ccgtcgtttg
    17941 atttgataac gatgtgttgt tccttttgcg taccaaggtc tttagctttt acagaaacaa
    18001 taccgttttt atcgatatca aatgttactt caatttgtgg gattccacga ggtgcagccg
    18061 ggatatcagt caattggaag cgaccaagag tcttgttatc tgctgccatt gggcgttcac
    18121 cttgaagaac atggatatca acagctggtt ggttgtctgc tgctgttgag aagacttgtg
    18181 atttagatgt tgggatagtg gtgttgcggt cgatcaattt agtgaagaca ccacccattg
    18241 tttcaatacc aagtgacaat ggtgttacgt caagaaggac aacgtctttc acatccccag
    18301 tgataacacc accttggata gcagcaccca tagcaaccac ttcatcaggg tttacagatt
    18361 tatttggttc tttaccagtt tcagctttta cagcttcgac aactgctggg atacgagttg
    18421 atccaccaac aaggataact tcatcaattt ctgacaatga caatcctgca tctgaaaggg
    18481 cttgacgaac tggagttttc gtacgttcaa caaggtcacg agtgagatcg tcaaatttag
    18541 cacgagataa gctcatctcc aagtgaagag gaccagcaga accagcagtg ataaacggta
    18601 agctaatttg tgtttgtgtc acacctgaaa gatctttttt agctttttca gctgcatctt
    18661 tcaagcgttg aagtgccatc ttatcttgtg ataagtcaat accattttct ttcttaaatt
    18721 cagccactaa gaaatcaata attttttggt caaagtcgtc accaccaagt ttgttatcac
    18781 ctgctgttgc aagaacgtcg aagacaccat cacctaattc aaggattgat acgtcaaatg
    18841 taccaccacc aaggtcaaaa actaagattt tttcatcctt gtcagtcttg tccataccat
    18901 aagcaagtgc ggctgctgtt ggttcgttaa cgatacgttc tacttcaaga cctgcaattt
    18961 taccagcgtc tttagttgcc tgacgttgtg catcgttgaa gtaagctgga acagtaataa
    19021 ctgctttttc tactttttct ccaagatagt cttcagcata acctttaagg tattgaagaa
    19081 tcattgctga aatttcttga ggagtatatt ctttaccatt tgcagaaact ttttcagaag
    19141 ttcccatctt tgatttgata gagattattg tttctgggtt tgtcactgct tgacgttttg
    19201 cagcatcacc cacgataatt tcaccatttt tgaatgatac tactgaagga gttgtacgat
    19261 tgccttctgg gttagcaatg atttttgatt cagtcccttc aagaactgct actgctgagt
    19321 ttgttgtacc taagtcaata ccaataattt tagacatgtt aatacctctt attttataat
    19381 actttattac tttttatttc aatttatctt agtaagcact cgaattattt tacttccaat
    19441 tctaagcaat cactatagtt taatcagcaa gcgaaatagc ttctaacttg ctatctagtt
    19501 tactgtcatt acggacaaaa acgaaaactc atttacctta gttataaaca actaccatcg
    19561 ctggtcttag cagacgctcg tgcaatttat atcccttttg aaagacttct gcaatgctat
    19621 cggctggatg ttcatcatcg gctgggaggg tctgaactgc catatgaaag ttgtggtcaa
    19681 acgagtcgac ttctacttct tctacgcctt cttcctttag tgcttgaata aggctatcac
    19741 gagtcatttc taaacctttt ttaacatcat ctgtcaaccc ttcaacagcc aaggcacgtt
    19801 ctagattgtc aagcgatggt aagattgctt ttgccagatc ctgagaacga tagcgttgaa
    19861 gttgttgacg ctcttcacta gagcgacgct gtatattctg catttctgca tgagctcgca
    19921 agtatttatt ttcaaactca tcagcacgtt cgtttgcaag ttctaactct gatttttcag
    19981 gagtttcttc aataacttcc tcaactgtct cttcagtttc agtggcttcc acttcttctt
    20041 gtaaatcgtc ttttttaatt tcttctgaca ctgagtgcct ccttttaaat aacttattga
    20101 atactgccat tatcagtgaa cttcataatg gttagaattt aggtatcgat aataatcacc
    20161 taatttagca gccaatacct ttccaataat gtttaccaaa ctaacagacc tacgataatc
    20221 catatcaata ggaccaatca aactcaggag tccaaaccca cggtaaggaa tcaaaaattt
    20281 gtgagttaaa actgacacat ctgcaagtgc ggcttcttga ctatctgcca cttggacaga
    20341 agccatctca ccttctttca gactttgcct caaagaaatt gctacttgct gttcattatc
    20401 caaaaactga tacgttgata gatctgaata cgttaaagaa tttaccttgc cagcaacaaa
    20461 taccgtttcc aaaaacagct ctgaaaaaac ataatcaaat agttgtaaaa cattatccgt
    20521 agtcacaaaa tacttttgaa cgatctgagg aatttctgtc ctcaacttat aatgaatatc
    20581 tatgacacta ttatccagca agcgctcctc tacgatagct ttaaatgcta tcaagtcacg
    20641 tgtcaaaaag tttcttggaa tagcaaactg cactgtcact ggcttagatt catctaaagt
    20701 catgactgct agagcatcat ggttgctaag ctgaacaaca tcaaaccctg ttaaacgttg
    20761 acgagctggc tccacatcta aaataacgga tgtatagccc gtcatctccg ctaaaatgtg
    20821 actagctttt tgaagcatgt cttctaattt gaatgcttca aaatcaaaag cttttatgac
    20881 atgataaata tcttgctcat caatactatc tagcctgagg gagtgctcta caaaatactt
    20941 aaaaccagct ggacttggca tccgtccact agaagtgtgc gctttttcca acaatcccaa
    21001 cttctctagt tttgccatat cattacgaat agtggcgcta ctagaatcaa tcgttctctg
    21061 taaagcttta gaccctactg gttcatgagt ttgtgtaaat agctcaacga ttaaattcaa
    21121 aatatcattt tgacgttgtg taatcacaaa ccaaccactc cctttcgttt agcactctat
    21181 ttagtagact gctaatttat aattctatta tactctttat gggagacatg tcaagaaaaa
    21241 aactcaaaaa aattagcact cttttataaa gagtgctaat ttcagtcata gtttgttaaa
    21301 tcgttacttt caatgacctg aattagtcgc ctagcatagg ttttatcagt ataatgcaaa
    21361 tacttttgta aacttttagc tggtgcttta tacccttttt ctgtagcaag attacgatac
    21421 aaacgcttat cgacttcttt gccctgccga agcattcgta aataatctct aattgcacta
    21481 gcatcatctt tataaatagc atatctcacg ttttggttgg tttgtttagg ggactttagg
    21541 gtaatggctg cttgtcctgg agttgctttc ttgctaaaaa gattatgata cttagacgct
    21601 agtaatgttt ttccatcatg ggtttccaaa agaatttgag caatcaaaat agatggtcta
    21661 atgccataat attttgccaa tggtttaact tctttaccca tttttgtaat aaattgtttt
    21721 tggctgtagt tcacccttac ttctttatct gcactagcta tcctttggct actcagtaag
    21781 ggacaaacaa tcagcaataa aaagcaagct agaagcgtta aaaaatacgg aaactttagt
    21841 cgttttctca tcgtttctcc tttaaagcag caatgtactc ttccaacgtt aggttgtgct
    21901 gagccatata acgagcagac gccttgccta catagcggta atgccaatcc tcataatcaa
    21961 cccctgtact agtctttttt ccttctggaa agcgcaagac aaacccataa tcaggtgcaa
    22021 ttttttgaac tgctttagat accgatgggt cactggcatt taaagtatct acggtgctta
    22081 tgtcaatagc aagaccagtg tgatgttcac tagcccctgg cggctgtaaa taggtttgca
    22141 ccaaagcctc cgcagattct tgtgtcaatg ttggatcgtt agccatctct tttttaatat
    22201 aagattggta aagctctgct tggtaagcta cggagcgata gcctgaaata aggtgctctt
    22261 gtgggtcaat agcttgtgct gctgctaaaa agtcactagt ggcttgctca atgcgtttat
    22321 ccacagaaat accattaatg tcagctaatt caggactcat ttcttttgta atgtgatcgc
    22381 ggtttactaa aaccaactcc caatcttcaa cagatacctt aggtaaagct cgtaaacggt
    22441 ccgttttttt gatatctttt tgttggattt cttttttatt caactgagtt ggtgttactg
    22501 actcttctgg ttttataaag aggtaaagcc ctcctaaaaa aacaataaca attagcaatt
    22561 gcactaaata gtttaacgaa ttatatttca tctgaccgtt cttctattat ttcacgaccg
    22621 cggtagcggt aactcatatc tccaatagtc tttatcgtaa attggccatc atcaaattca
    22681 acaactgtaa cgctcccatt atcaatatac tgtttgtctc gatctggatc aatcaaccac
    22741 agaaacgttc caattgtcat accatggcta accacaagag catttccgcc gccagcattt
    22801 tgaatggaca aggcaatagc ttcaaacccc tcccaaatgc gcttacgaag aacttcccat
    22861 ggttctgccc aattagcggt atctatttcc acgatgcttt ctgccagttc agaataggga
    22921 acatcacgta ggttatcacg attttcaaag gcttttgttc ttggaaggac acctagaaat
    22981 aattctgaat cataagtccc ttctagactc ccaaaacacc actcacgaat acgattatct
    23041 ttagtataag gcaaaaattc gttttctgat tctcttaaaa taatttctat tgtctgcatg
    23101 gttcgacctg aatcacttga aaacgcagcc ttaaatggta tagttgcatc ttttaaacct
    23161 aagccaagtt ctcgaatacc ttcttctcct ttttttgtaa gaggtgtgtc actccaaccc
    23221 tgagcgcgac caatggtatt aaacatcgtt tttccatgtc tggcaatata caaccttgtt
    23281 tttgtcattt caacctccta aactgtctta ttaattatta tagcatttaa agaaaacact
    23341 tgcaaaacac ttcctgatgg ctatttgaga tcaaatagac cactgactta ttgtctattt
    23401 tattaaaaat aatgctattg ctacaattca aatcatcaca taaatttcca aacgattaca
    23461 tttgtgtgtt tcgctcaaat gtatataaca acagacccac actacaacta aactgacgtt
    23521 acaaaatgat gtcaaaacag taacaaaatc taactatttg tcaagaaaac gatattaaat
    23581 tttcacacaa ctatcttata atgaggatgc aaccaataaa aagataagac attttatgtt
    23641 gcaaaaaagg ctgcttggaa acttccatgc agcctttttt taataccttt ttccaaaaat
    23701 agctgaaaca agtacaatta caattgctgc tcccaaaatg gacgggaaaa tagccatacc
    23761 tgccaactga attccccaat ccccaaaaag cctttgccca acagctgacc ctatgagacc
    23821 tgcgataata ttagtaaaac aacccatgga ctggcttttt tcagtcattt tacctgctat
    23881 catgccgata aaggcaccta caattaaaga ccatatcatc attaacacct cctttcttgt
    23941 aaaatccttt tagaagaact gctatccatt aaatgttctt aactttagtt gattccttta
    24001 ccttcttctt tagtaagtgt ttccgtgcct gagtaaaata gaaaaaacaa ttccttctat
    24061 taagatagat cgagaacgac tagaagtctt cgcacactaa ataacttaac aaactttatc
    24121 acacagctaa tcgctaataa tagtgtagac aaacgtgatg gttcattaaa tacccgtcag
    24181 aaactcctga aggttctaaa gcaatacctg tcagtcttat tttagcatag atgatggtct
    24241 ttttataggc ggtcactaac atgacataca aaaaggcagc ctgtgaaaga ccgaccgcct
    24301 ttttttcaga gacaaaatac tttaacttat tgctatcagc aactatcaat ctagcaattt
    24361 ttgcaattct tgtgccaata attgttgagc gacttgtgga ttagcttgac ctttggttgc
    24421 cttcatcaag aagcctgtga aagccttatc agcattacgt ttgcctgatt tgaaatcagc
    24481 cactgcagct tcattatcag caaagacttg gtgaataatt ggtaccaaaa cagcgggatc
    24541 agaaatctga acaagccctg ccttttcgac gtaggcgcga gctgagccac cgtttttagc
    24601 caaatgcaca aagaccttct tagccatttt agaagagatt gtaccatcag caatgatagc
    24661 aatcatctcc accaaatttt ctggtgtcag agcgatttct tcaatggttt tgccttctgc
    24721 atttaagaat tgggcaactt ccccttgcaa ccagttagag acttgcttgg cgtcgccacc
    24781 aagacttact gcagtttcaa agaaatctga caaggctttg gtagctgtta gttggctagc
    24841 atcataagtt gagagaccaa gttcctcttc gtatttagca cgacgttgag ctgggaattg
    24901 aggtaattga gcacgcatct catcaatcca agcatcatca atttcatata agggtaggtc
    24961 tggttctggg aaataacgat agtccgctgc cccttctttg acccgcatca aaatagtccc
    25021 cttattcgct tcgtcatagc ggcgtgtttc ttggcgtatc acaccacctg aacgcaagag
    25081 tttggcttga cgttccacct caaattcaag ccctttacgg acatttgaga aggagttcaa
    25141 gtttttcaat tcggtctttg ttccaaattg ttcttgcccg taaggacgaa gagaaatatt
    25201 ggcatccaca cgcatggagc cttcttccat cttgacatca gaaatacccg tatactggat
    25261 gatttctttc agagctgtca aataagcata ggcttcttca ggagaacgca tgtcggcttc
    25321 tgacacgatt tcaatcaaag gaactccttg gcggttaagg tccacataag aataaccatc
    25381 tgtgccatga gtgtttttac cagcatcttc ttccaagtgt gcgcgttcaa tacgaatttt
    25441 cttggttgag ccgtcttcca atttaatctc aatccatccg ttgtagccaa tcggctcgtc
    25501 aaactgtgaa atttgataag ctttaggatt atctgggtag aaataatttt tgcggtcgaa
    25561 atgcatctct ttatggatgt ccatattgag ggcaagcgca gcttttatgc ccgcatcaat
    25621 aacgcctttg ttcatgactg gtaagacccc tgggaaagac caatcaatca cattcgtgtt
    25681 ggcatttggg tcttcaccaa agtgagctga cgaaggtgag aaaattttgg aattggtatt
    25741 taattccaca tggacttcaa gcccaataat ggtttcaaaa ttcattactt gtcacctcca
    25801 aaaataatcg gttgttgctt gtgataatct gtcaccgctt caaaggctgc tgctgcctga
    25861 tagatggttt cttcagcgta tttcggacca attaattgaa gccctactgg caagccatcg
    25921 acgaatcctg ccggaataga aatacctggc agaccagcta agttaaccgg aatggtcaat
    25981 aagtcggcca aatacatggc aacaggatca tggttgagtg tgtctaagcc aaaagcaacc
    26041 gtcggtgttg ttgggcctaa aataagatca taatccgcaa agaccttgtc aaaatcttga
    26101 ataatcaaag tgcgcacttg accggccttt ttgaaataag cgtcataata acccgatgac
    26161 aaactaaagg ttccaagcat gatacgacgt ttcacctcat caccaaagcc ttggctacga
    26221 gtgttaacat agatttcatc caagttttta gcatcgtcag cacggaaacc atagcgaatg
    26281 ccatcaaaac gttgtaagtt tgaagacgct tctgacgaag caataatgta atagacagcc
    26341 acaccatatt tgctatgtgg aagactaact tcctcaacag tagctcctaa ggcttcaaat
    26401 tgtttagctg ccgcaagaac cgtttcctta atctcaggat caatcccttc gccaagatac
    26461 tctttaggaa gggcaatctt catgccttta atgtcacgac caatcttaga tgtgtagtct
    26521 gcaatgcgga caggcgctga agtggcatcc ttgacatcgc tacttgcaat cacatttaat
    26581 aattgggcgt tttctttgac tgtcggagca aaaggtccaa tctgatcaag tgaacttcca
    26641 aaagcaatga gaccatagcg agaaacagtg ccataggttg gtttcaaacc gacaacacca
    26701 ttaaatgcag ctggctgccg aatagaacca ccagtgtctg aaccaagaga taaacggact
    26761 tgtcctgaag cgactgctgt ggccgaacca cctgatgagc caccaggtac ttttgtgtgg
    26821 tcccaagcat ttttcgtttt cttgaaataa gaggtttctg ttgaaccacc catggcaaat
    26881 tcgtccatgt tggtttttcc aataacaatc atgtctttag cgtaggcatt tgctactgat
    26941 gtggcattaa aaatcggctc gtagttatac aacatttttg aagcggctgt ggttaggatt
    27001 tccttggttg aaatattatc cttaacagct aacggaatac ctgacatgag gttatcagca
    27061 tcaatacctt tggcatcaat tgccgcagct tgtttaagag caacttcttc tgaaacagtg
    27121 ataaacgatc cgacagcctc ctcacgagac ttgatgtctt caagtgttgc ttgggtcaat
    27181 tctgttgctg aaatctcctt agctacaagg aggtcatgca actcttcaat ggttttatga
    27241 ttaaaagaca ttaggcatct cctccgtcat ccaaaatagc aggtaccttg ataaagtgat
    27301 tttctttttc aggaacattt ttaaagagca aggcacggtc tgttccttct tcagcgacat
    27361 cttgacgcat cacatttttc ttatctgcca tggttgttgt aatggcgaca ccttcggtat
    27421 caacttcgtt cagcaattca accatatcaa cgattttgga taaggtcgtt gcaaaggttg
    27481 ttgtctcact ttctgaaaat gataattttg atagcttcgc gacatggcga acttcttctt
    27541 cagaaatttt catgatttct cccatccaag atgatcagaa gtattccaat tccaaagaac
    27601 agccctgcta ttctccaaag gtagcggaaa aactcctcac atcattttta catagtctca
    27661 attattatat cacaattgct cccatttacc ataggtgact aatgcaattc tagaaagcca
    27721 tacagcaaaa aacttcctga ctaaggtcag aaaaccttag tcaggaagtg tgacaaacta
    27781 aactctattt gtcttgggta agactgtggc ctgaagcttt gatggcagcc tgagcagccg
    27841 ccaagcgagt aagcggcact ctaaatggcg aacaagaaac gtaggtcagg ccttggctgt
    27901 ggcaaaaggc aatggaagca ggatcgccac catgttcccc acaaatacca agtttcaact
    27961 caggcttaac cgaacgggct aaagagacgg ccaatccctt tctgatcaaa ggtctggaac
    28021 ggctccttat caaaaatacc cctgtcaacg tattcttcca agtattttcc tacaaacatc
    28081 acagaaaaag taacgttctt tcaagtcggt aatatgttta cgaaggtagt aaaggggtgc
    28141 tttattctaa tttaccaaat cctcaacgat tgtaacaaca tgtgatgtga taagcctaag
    28201 ataaaaatgg tacagactct agcattcaag agtttgaagc tttatttata ttctaatgct
    28261 gaaaagacta gagtttgtct aatggctgtg agacgatcct ctaaaccatc aactcaaatc
    28321 atttctcaaa cagctaaaat cctgtctcct tcaaaaaaac atgtgttcta tctgacacag
    28381 tccaaagttt agttgccttt tagacagcac aagaatcatc aggactaatt ctgaccagca
    28441 tgtcacatac ctcatctacc taacccatac atacggtaaa accacataga gcaatgatta
    28501 cggttaccaa actcttagtc actgaatcaa tagtttggta ggctctttta gtaatcgtta
    28561 ataagcttag caccaagaac ctgctcaaag tgagccaaag accactcata agattctttt
    28621 gtaaggctag cgacagccga tttaacaatc tctaattgat aaccaagatg ataagcgtca
    28681 atagcagtat gtaagacaca gatatcagat aaaactcctg ttaacactaa ttgagtgata
    28741 cctctttctc gtagtcgaat atctaaatca gtccccgaaa aagctgaata ataacgctta
    28801 tcaatccaaa aaactctggg atgttgcttc atatgactgt aaacttcagc tagtggacca
    28861 taaagatggc gtccagtagt tcctttgata ttatgtgctg caaatagctt actctcagga
    28921 tgccaaggat cattttgatc atgacaatca atagcaaaaa aaatatagtc tccttgatca
    28981 aatgccgttt tagttacctc tgctatttta gtggcaatag cctgagcaga tttacctgca
    29041 cttaatttac catcatctgc tacaaaatca tttgtgtaat caattgaaat caatgctctc
    29101 atcactatct cctaaacagg gaaacctctc cccttatctc ttatatatgg cattgtatca
    29161 gtttttatta caggtatcaa gacatctatt tgcatctacc attaacagca aaaaacatct
    29221 cttttcaaga gatgttgtct ttttctattt aaaattcttt taatttagca aaaatacctt
    29281 cgttgataac tttgaggtat gtccctttca taccaagaga acgactttca ataatccctg
    29341 cactttctag tttacgcaga gcattgacaa taacagaacg ggtaatacca atacgatcag
    29401 caataacaga agctgttaat cgtccttcat taccatctaa ctctccaaga attgctgcaa
    29461 cagctttcat ttctgaataa gaaagcgtat taattgccat gttgaccgct gtttgtttac
    29521 ggatggtgtc ttctaagttt tctgtctgaa gatttaataa ttgaatcccc acaacagtac
    29581 ttgagatctc aaccaaaatc aaatcatcat cactaaactc attgtcatta cgccagataa
    29641 tgagtgatcc aaggcgcatc cctccaccat aaatcggcgc aatagtcgtt agacctcctg
    29701 gataagtgtc tttagactca acagggaata tagtcaattc attttcgaca gaaaggttag
    29761 cttctgtatc gtaaactcgg ctagcagcct ttacataagt atctggaaat tgtttagctt
    29821 caaaaaattc ctcaacacga tcagtgttag ttttatattt catggcataa ccaagcagtg
    29881 tgccgccgcc attgataata caagcattgc agtcaatgat atctgctagg cgagatgcca
    29941 ttgtgttata cggtaattct gtttctaggc tatctacgga acgctgcaaa atagatgtga
    30001 ttttacgagt tttttctaat aagttaggca tttttttcct tttcctttga attatttgcg
    30061 ctttcattat aacaaagcta actcgctttt gcaaggaatt atcagaaaat tttctattct
    30121 tactacaatt ccaactattg atgccttatt atcacttcat ttaaaggaga taagcactag
    30181 cttgttagag catagggtca cattctttta agaaacgcta tttttattaa tcaaagatga
    30241 gcattgcatt aacgtctata ctggtataag acacgtgtaa tcttttcttg gacatcttct
    30301 aattcttcta cacttggtag gtaaacaata cggaagtgat ctggatcttt ccagttaaaa
    30361 ccacgtccat gtaccaacat cactttttct tgctttaata actgtaagac aaattcttca
    30421 tcatcatcaa tacgatacat ctgacgatca attttcggaa ataaataaag acctgcttca
    30481 ggtttaactg ccgataagcc agggatagca ttaatagctt cataaataaa attacgttgc
    30541 tcaaaaatac gccctcctgg cagtaacagt gcatcaactg attgatggcc tcctaaggaa
    30601 gtttgaacaa cttgttgggc taaaacattg gagcataacc gcatattggc aagcatgttc
    30661 aacccttcaa tgtaaccttt aacatggtct ttaggacctg ataagaccat ccatccaaca
    30721 cgaaaaccag caatacgatg agatttggaa aggccattca tagatacaca aaaaacatca
    30781 ggtgccaaac ttgcaatagc aatatgctct ttaccatcca tcaccaaacg atcatagatt
    30841 tcatcggcaa aaataatcag ctggtgttct cttgccaaag caacgatgtc ttctaaaatc
    30901 tccttaggat aaagggctcc tgttgggtta ttaggattga tgaccacaat agctttggtg
    30961 cgactcgtga tttttgattt gatatcagca atatcaggat accaaccagc ttcttcatca
    31021 caaagataat ggaccgcttt tcctccacca agactcacac aagccgtcca taaaggataa
    31081 tccggcattg gtactaaaac ttcatcgcca ttatctagca aagcttgaag ggacatggat
    31141 atcaactctg acacgccatt tcccaaataa atatcttcaa tgtcaacatc tgggaagccc
    31201 ttgagttggc aatactgcat gatagcctta cgcgctgaaa aaatcccttt actgtcagaa
    31261 tagccctcac ttagcctcgc gttgacaatt aaatcacgaa tcacttcatc tggtgcttca
    31321 aaaccaaaag cagcaggatt gccagtattt aaacgcagaa ttttttcacc actcgcaatc
    31381 atgcgattag cttcatccaa aacaggccct cgaatatcat aggcaacatg ttctaatttt
    31441 gatgattttt caataatttt catagtctca tacacctcgt attccatatt ataaaccaaa
    31501 atacttaaaa tagaaagaat ttatccatca aagcttagga cagctaatcg ttttaacaca
    31561 ggtcttatga gacgataata cagtctttag tttagcaaaa ataatggatc gctttcaaaa
    31621 tgactcaaaa aagagtataa tgaaaaaaag aataaaagct attcttgtca aactgttaaa
    31681 ggaggacagt tctatgtcac taaaatataa acgtatcctc gttgctattg atggctctta
    31741 tgagtctgaa ttagctttca ataaaggcgt taacgtagca ctacgtaacg acgctacctt
    31801 actactagta cacgttattg atacccgtgc cttgcaaagc gtagctactt ttgatactta
    31861 catctatgaa aaattagaac aagaagctaa ggatgtttta gatgattttg aaaaacaagc
    31921 acagatagca ggaattacaa acatcaaaca aattattgaa tttggcaatc ccaaaaactt
    31981 actagctcac gatattccag atcgtgagaa cgctgattta attatggtag gagctactgg
    32041 tcttaatacc tttgaacgcc tccttattgg ctcatcttct gagtacatta tgcgacacgc
    32101 taagattgac ttacttgtag tccgagatag cactaaaaca ctctagtcta ttacaatttt
    32161 caaacaccaa aaaacaagca gcatctacaa cgaaattgct tgttttttgt gctgagctaa
    32221 ggctagtcaa tatcttttta tttcttgata tttaaaagag taaacctcta tttatcgaat
    32281 cgttgtagtg atttttgtaa ttgtttgtct aactcttttt ttatggctac ttcaggtgcg
    32341 tgacgctctt gccaatagtc aggtttttga attttatggg taacggggtc aaagtgtgcc
    32401 ttgccatctg gaaaaatttt agccatattt gcttcatgta ctgcttcaaa tataggttgt
    32461 ggatctactc ccatcagtac aaaagaacca taagtaaaat aaagcaaatc cgctaaggca
    32521 tcaacttgcc caatcagggg cgtttcaaca tgttttttag cttgtacctt attagctgct
    32581 tggtcaacag caccatgcaa atcgaaaata gcctgtgtaa attgttgttg gtctcccttg
    32641 cttgctgcgt aaagaaactc aacgatttct tctactttga agcctgacct ataaccagct
    32701 tctgaaatgg tataactacg cggtgtttca atagtatctg agtccatcag acgatgaaaa
    32761 tctttgactt tattaaaatt ttcatcacgg cttttaaagc tcttctctac atcaaaatgg
    32821 atgagaccgt agtgagctaa ggctttggaa atcccatcat tattattaga gtcggtagtg
    32881 aaatgggctc catcttttac cacagtttca gcatttccca tagcaacacc gataccaact
    32941 cctgataaca tttcaaggtc attatctgaa tctccaaaag ccatcacttc agacaagtca
    33001 aatccaaaca tctctcccaa acgttcaatt ccttttattt ttgattgatc aacagagatc
    33061 agatccaagg aataaggact acttcttgta atttttatat gagggaactt ctcttgtatt
    33121 ttcttagttt cagcttgtga cgctaccaga acaacctgat aaataggctc ccgcataata
    33181 gttactaaat ttctaaaact ctgtggcttg attcggcgaa tcaaatgctt aaaactaccc
    33241 tcaactgttc ttgcccaact tttgggcaca aaactactaa tgacttgtcc aaatgggcta
    33301 gtccccatat caataatacg cgatcctgct aatccagaag ctgttccaag agaaatttct
    33361 cgcttttttt cgttggcata gcgaatcact ttgtaaatca tcgactttgg caattgattc
    33421 tgatataaga ccttatctct tgttaagata tattggccat tgtaagtaac ggcaaaatct
    33481 aatccaaaat tttctagaaa aggttgcaca aatcccggtc cacgaccagt tgctaaaccg
    33541 accataatgc cttgtttttt gagttgttgg atggcttttt gcgttgtttt ttggatattt
    33601 ttacgatcat tgagaagggt cccatcaata tcaaaaaaga ctgcttttac tgtcaaaacc
    33661 ttcttttgcc tcctgtgttt atgatagaat agattatacc ataaataaaa ggaagtttga
    33721 caatcaaatg aaaaaaatcc tagtcttaca cactggtgga accatttcta tgcaagctga
    33781 taactctggt agggttgttc caaatcaaga taatcctatg acgaaaatac atgctgcagc
    33841 ccaagatatt cagcttaccg ttagtgattt tctaaatctt ccgagtcccc acatcacgcc
    33901 tcaccacatg ttatctattt atcatcatat tcaagaaaga actgatgtct ttgatggtat
    33961 agtcatcaca catggaacag ataccttaga agaaacagct tattttttag ataccatggc
    34021 attgcctact aatatacctg ttgtcctaac aggtgctatg agatcttcta atgaagttgg
    34081 tagtgatggc atatataatt accttaccgc acttagagtt gcaagtagtg ataaagctaa
    34141 agaaaaaggg gtacttgttg ttatgaacga tgaaatccat gctgctaaat atgttactaa
    34201 aacacataca accaatattt caactttcca aacacccaca cacggaccgc ttggtatcat
    34261 catgaaaaat gacctacttt ttttcaaaac tgctgaacct cgtattcgtt ttaacttgag
    34321 atgcatttct ggaaccatcc ccattatcaa agcatacgca ggtatgggcg atggtagtat
    34381 cttgagtcta ttaactccag gtagcattca aggtctagtt atcgaagctc taggggcagg
    34441 aaatgtccca ccattagcag ttggggagat cgagcactta attactttag gtattcctgt
    34501 cgttcttgtt tcccgttgtt tcaatggtat ggcagaacct gtctatgctt atgaaggtgg
    34561 tggtgcgatg ctacaagaag caggtgttat gtttgtaaaa gaattgaatg ctccaaaagc
    34621 ccgtctaaag ttattaattg ctctcaacgc cggacttact ggtcaagagc taaaagatta
    34681 tattgaagga taatctagct tcctgtagaa atgtcccact aaaacatatt agtgggacat
    34741 ttctagtact ttaagcttta aattaggaca tttcctaaat gtctataata aacaactatg
    34801 tttcaaacta tgagattgtg cactcataat catttctata acagtttgag accagtattc
    34861 caataaatcc aactcttagc aatcagatac tgggtaaaca agtctttatt ttgtctcatt
    34921 aagtatgagc ttacttgaat aataacaaaa ctacttgctc atcctttttt agtaatgatg
    34981 aagacgacct ttttctaaga acttctttgt cttttaaaat aaacaaagca catattaccc
    35041 tttattttag ttctcgtttg gaaaaaagaa agacattgcc taaaaaataa caaagagtta
    35101 aaccaaaggc cactaatatg ctagtactag aaaggtgttt atcaccaaga gacaaggata
    35161 ggtgtttaaa catcaaaact gggctaatat ctaacttaaa aaaatggttt gctaaagtag
    35221 cagatacttt atcaaaaaga agaacataaa gtatataagc tgtgattgac tgataagttt
    35281 tacgaagtat attaaacaaa aattgacaca aagcaataat agcaatataa aaaggcagtt
    35341 gtttaatcat ccgatctatg actattccat agtatgcttg accagtaaat aaatgaaatg
    35401 ctaaagcact aagaagataa gttaaactaa taaataaaag agataagcca aaactaacta
    35461 gcagtttata gattataatt aaagtacgat tttgttgttt gaccacaaca ttattaatcg
    35521 tacggtagtt atagtcatca ctaaaaaaga tacgggctgg tgttaaaaag aaaagcgtca
    35581 caaaatcagt aataatatta atgatttgta tcatgatgac ttggttaggt gtcttgcgaa
    35641 aactataggc tacaccaaca gcaatgacaa caagtagcat acaagaaaat agagctaaac
    35701 gctcttggcg tatccgaaat aaatctgctt taagatagga aatcataaaa acctctttct
    35761 aaattaatgg tgacgctgca tctttaccta acaaatcgag gtaataatgt tcaaagctca
    35821 cttgatgatg ataaatgtca aaaatctcaa caggcagatc tagaacaatt ttgataagct
    35881 ccattactga taaagttggt tttattagta gcatctgacc atcagtctca actctaagcc
    35941 ccaattctaa aagtctattt tttaccagct gatcatcgct tgtatgaagg gcaatttgta
    36001 cttttacttg agctttaagc tctgctttgt ccatactctt aataactttg cctttatgca
    36061 taatcacgta acgatcaaca actaagtcta attctgataa aatatgactg gaaatcaaaa
    36121 tcgtaatacc atagcaatct cttaggttca aaataatctg acgcatttct ttgattcctg
    36181 ctggatcaag accattaata ggttcatcta agattaaaaa ttctggaaaa tccagtatcg
    36241 aaagaccaat agccaaacgt tggcgcatcc ccaaagaata atctttcacc tttttctttt
    36301 tgctcacttt ttctaaccca ataagttcca aaacctcatg tatgcgctct tttgcatcag
    36361 ctaatctcaa ttgcaacgct gcatagtaaa gattatcatg agctgtcata ttgggataaa
    36421 gtgtcggtga ctcaataatt gatcccattc gatagggttg taactgtttg atttgccctg
    36481 aatcttttcc aattaggcca gataaaatac gcattaacgt tgtctttcca gcaccatttt
    36541 gacccacaag gccgcaaatt tctcctttgt aaatatcaaa agagatatca cttaaaacct
    36601 gatttttacc ataggattta tttacttgct caatacttag taacattgat tgctgtgtca
    36661 ttttccgctc cttttttgta cttatcgatt aatacaatat cttacacata ataacatctg
    36721 taaacggttg tgacaagttc ttctttttat taaaaaaata taaataagaa ttttaataat
    36781 atgattattt tagcaataaa aaagttatac tgaaaatggg ttaatactgc ttcaaatagc
    36841 agtctcatcg taaagcataa gtaaaaaagg tcagaaatag tgacctcttt atactttata
    36901 acagtttatc tcaaagctaa ctaaatattt gtaaaacaac gacttgtttt aagttaatca
    36961 taaacttctt tttttctaat attttttgct actaattgcc actgtttttc atatatccag
    37021 ttaggatcag aaacaatcgc tgcagaaact ttgcgtgctt cttctaaaat aggataatcc
    37081 tcaacgatat cagctacttg aaattctgga attccagact gacgagtacc aaagatttca
    37141 ccagaaccac gcatttttaa atccgactca gccaaaacga aaccatctgt cgtttctgtc
    37201 atgattgtca ttcgtttttt ccccgaatcc gttttgggat tagccactaa aacagcgtat
    37261 gattgtttat gtccacgacc aacacgccca cgaagttgat gtaactgact tagtccaaaa
    37321 cgatcggcat ccataataat catgatcgtt gcatttggga catttacccc tacttcaata
    37381 actgttgtgg atactaaaat atgacttttt ttatccttga aatcttgcat tatggcatct
    37441 ttttcatcat ttttcatacg tccatgtaca agagcaacct tagcaattcc ttcaaaataa
    37501 gtagataatt cggcatgcaa tgctactgca ttctttaaat ctaaagcttc tgattcttca
    37561 atcaacggtg aaatgacata cacttgagca tctttttgca attcaccttt tacccattcc
    37621 aacacagtcc ctagctgctc gtgtttcacc cagcgtgtca taataggttt acgaccggca
    37681 ggtaattcat caataacaga aacatccatt tctccaaaag ctgtgattgc tagagttcgg
    37741 ggaattgggg tggctgtcat cattaaaaca tcaggatttt ctcccttttc acggaaaatt
    37801 ctacgctgtt taacaccaaa acgatgttgc tcgtctgtaa tgacaagccc cagtttatgg
    37861 tactgtaccg agtcttggat aagagcatga gttcctacaa tcatatccac tgagccattt
    37921 gcaatagctg ctaaaaccgt acgcttgaca gcggctttca tacccgaagt taatatagca
    37981 attgatagat ctggaaataa ctcttgcaga ctaatgtagt gttgttcagc taggatttcc
    38041 gttggaacca tcaaagccga ttgaaaacct gctgtataag ctgcatacat tgatagacca
    38101 gcaatgactg tctttcctga tcctacatct ccttgcaata aacgattcat atgagctccc
    38161 gatgacatat cagataatat gtcatctaaa gagcgctttt gagcatttgt tagaataaag
    38221 ggtaaagagg aaatctttgt ctccatagca tgtttactat aaagaatagg caaaccattt
    38281 gtttcagatt tattttcggc tttcaaaact tgaaggttca tttgaaagta aaataattct
    38341 tcaaatttaa tccgacggag cgcttgctta tactctgtga tatcttttgg gaaatgcata
    38401 gctaaacaag cctgactacg acccatcaat cggtattttt ccaataaagt agctggtaaa
    38461 ttttccttca attccaaatg cgcatcgatt tcaaaagctg acttaatagc tttaatcaaa
    38521 gtagactgtg aaattccctg agctacgcga taaacaggtt gcatgtcatc ttcaacttga
    38581 gctaaaacct tcatcccagt aatagccgat ttagtggcat cccatttacc aaaaacagct
    38641 acctcttgac caagttctat tttatcagct aaatagggtt gattaaaaaa actaacattt
    38701 aagacagctt ccccttgacg caatttgaaa cttaaacggt tacgtttaaa accataatat
    38761 tgtacattag ctggagtaac gactaagcct gtaatgactg ctttttcacc atccactaaa
    38821 tcaaaaacag attttgattt aaaatcttca tagcgaaacg gataataaag cagtaaatct
    38881 tctactgtat aaatatctaa tttctgaaat ttttctgctg attttggtcc aaatcccttt
    38941 aagttggaca taggagctgt taatatcatt atctttttcc cttcacaaaa actcttgtca
    39001 gattgaagac aaagtttgtc gtgatacttt ccttaggtgg tgcttacggc agcgacgttc
    39061 tttggaattc cctgcc
//
LOCUS       DAUCLR010000007        36851 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.007, whole genome shotgun sequence.
ACCESSION   DAUCLR010000007 DAUCLR010000000
VERSION     DAUCLR010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36851
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.007"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(277..816)
                     /gene="nusG"
                     /locus_tag="VGT87_000266"
     CDS             complement(277..816)
                     /gene="nusG"
                     /locus_tag="VGT87_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003100341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEQ3813158.1"
                     /translation="MLDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEI
                     PTQTVNVEKNGQTKEIEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKP
                     TPLLEEEIRAILLSMGQTIDVFDTNIKEGDVVQIIDGAFMGQEGRVVEIENNKVKLML
                     NMFGSETVAEVELYQIAEL"
     gene            complement(989..2032)
                     /locus_tag="VGT87_000267"
     CDS             complement(989..2032)
                     /locus_tag="VGT87_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEQ3813159.1"
                     /translation="MNKKVMSLGLVSTALFTLGGCTNNSAKQTTDNSLKIAMITNQTG
                     IDDKSFNQSAWEGLQAWGKENKLEKGKGYDYFQSANESEFTTNLESAVTNGYNLVFGI
                     GFPLHDAVEKVAANNPDNHFAIVDDVVKGQKNVASITFSDHEAAYLAGVAAAKTTKTK
                     QVGFVGGMEGDVVKRFEKGFEAGVKSVDDTIKVRVAYAGSFADAAKGKTIAAAQYVEG
                     ADVIYHAAGGTGAGVFSEAKSINEKRKEEDKVWVIGVDRDQSEDGKYTTKDGKSANFV
                     LTSSIKEVGKALVKVAVKTSEGQFPGGQITTFGLKEGGVSLTTDALTQDTKKAIETAK
                     KAIIEGTITVPEN"
     gene            complement(2359..3651)
                     /locus_tag="VGT87_000268"
     CDS             complement(2359..3651)
                     /locus_tag="VGT87_000268"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEQ3813160.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSADAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPQKISVIGNGVVVNPKSLVKELAYLHDEGVTTDNLRISDRA
                     HVILPYHIQLDQLQEDAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFAERL
                     RINLAEKNRLFEKMYDSTPLDFDAIFEEYYAYGQEIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGKRIDYYPASLEQLKRCKPIYEELPGWQEDITGVRSLDELPEN
                     ARNYVRRVGELVGVRISTFSVGPGREQTNILESVWASI"
     gene            4089..4967
                     /locus_tag="VGT87_000269"
     CDS             4089..4967
                     /locus_tag="VGT87_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813161.1"
                     /translation="MIRKYDRTSTKKKSLNWIWLIIAFFMISSFIGGSSFTESLLDIL
                     PAIAIGGTGYAIFRVRSHQKRLAKAKVAKQLEDLKAKIQLADRKVRLLDTYLADHDDF
                     QYNVLAQQLLPQLSDIKAKAITLKDQLDPKIYRRITKKANDVESDITLQLETLQIATT
                     LNPQPLKTPSPNLINKAPELKPYYDNIQTDHQAILAKIQGADNQEELLALHEANMRRF
                     EDILTGYLKIKEEPKNYYNAAARLEQAKQAIQQFDEDLDETLRRLNESDLKDFDISLR
                     IMQGATQRRTTHHQKD"
     gene            4980..6242
                     /locus_tag="VGT87_000270"
     CDS             4980..6242
                     /locus_tag="VGT87_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEQ3813162.1"
                     /translation="MADFNFDIDQIADNAVVKTDKTTDIISDLPTDTNGQISFFEKLS
                     ADQQTAITAKAPALVDTFLADQNALLDFGQSAVEGVNATVNHILAEQKKLQIPQVDDL
                     LKSTNRELNGFIAKYKDATPVDLDKKPNFLQKLFKQSRDTLQEFYFDSQNIEQKMDNM
                     AAAVVKQEDTLARNIVSAELLIEDNTKSIEHLVGVIAFIEASQKEASQRAAALQKDLK
                     TKDSTTPDYQIKADLLARTTEVINTLEQQHTEYLSRLYVAWATTPQMRNLVKVSSDMR
                     QKLGMLRRNTIPTMKLSIAQLGMMQQSVKSGMTADAIVNANNAALQMLAETSKEAIPA
                     LEQSAQSPTLSMKSVTSLAESLVAQNNGIIAAIDHGRKERAQLESAIIRSAETINDSV
                     KLRDQNIVQALLSEGKETQKTIDKTTHA"
     gene            complement(6362..6988)
                     /locus_tag="VGT87_000271"
     CDS             complement(6362..6988)
                     /locus_tag="VGT87_000271"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEQ3813163.1"
                     /translation="MARLNVKPTRMELSNLKNRLKTATRGHKLLKDKRDELMRRFVDL
                     IRENNELRQTLEKELAANMKEFVLAKASENSLMVEELFAVPVHEVTLWIDIENIMSVN
                     VPKFHVQSNTAREQEQGEFAYSYLSSNSEMDNTIQKTKELLEKLLRLAEVEKTCQLMA
                     DDIEKTRRRVNGLEYSIIPQLEETIHYIELKLEEAERASLVRIMKITS"
     gene            complement(7033..8448)
                     /locus_tag="VGT87_000272"
     CDS             complement(7033..8448)
                     /locus_tag="VGT87_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEQ3813164.1"
                     /translation="MSVLKEYRTVSEVVGPLMIVDQVAGVHYNELVDITLHNGERRKG
                     QVLEVQGDKAMVQLFEGSTGINLAKTKVRFTGHPLELAVSEDMVGRIFDGMGQPIDGG
                     PELIPEKYLDIDGQAINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDDNFAVVFAAMGITFEEAEFFMNDLRETGAIDRSVLFINLAND
                     PAIERIATPRIALTTAEYLAYEKGMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLIGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSQE
                     LYKNGFRPPINVLPSLSRLKDKGSGEGKTRQDHAATMNQLFAAYAQGKQAKELAVVLG
                     ESALSETDKLYVAFTNRFEEEYINQGFYTNRSIEESLDLGWELLSILPRTELKRIKDD
                     MLDRYLPKADTTMTKVFVAND"
     gene            complement(8449..10224)
                     /locus_tag="VGT87_000273"
     CDS             complement(8449..10224)
                     /locus_tag="VGT87_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEQ3813165.1"
                     /translation="MNQGKIITVSGPLVVASGMQEANIQDICRVGHLGLVGEIIEMRR
                     DQASIQVYEETSGIGPGEPVVTTGCPLSVELGPGLISEMFDGIQRPLDRFQKATDSDF
                     LIRGVAIPSLDRKAKWAFIPKLSVGQEVVAGDILGTVQETAVIEHRIMVPYKVSGTLV
                     AIHTGDFTVTDTVYEIKQEDGSIYQGSLMQTWPVRQSRPVAQKLIPVEPLVTGQRVID
                     TFFPVTKGGAAAVPGPFGAGKTVVQHQIAKFANVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLQEMPGDEGYPAYLGSRIAEYYERAGRVRTLGSQEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYQDDVGSYIDRKQ
                     QSNWSNKVTRAMAILQREASLEEIVRLVGLDSLSEQDRLTMAVARQIREDYLQQNAFD
                     SVDTFTSFPKQEAMLTNILTFNEEASKALSLGAYFNEIMEGTAQVRDRIARSKFIPEE
                     NLEQIKGLTQKVTKEIHHVLAKGGI"
     gene            complement(10425..10745)
                     /locus_tag="VGT87_000274"
     CDS             complement(10425..10745)
                     /locus_tag="VGT87_000274"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEQ3813166.1"
                     /translation="MTKTLYKVGVIGNRDVILPFQMIGFQTFPVIKPQDAINQLRQLA
                     MEDFGIIYITEDIAAAIPEALTHYDNQVLPAVLPLPTHQGAQGIGLSRIQAMVEKAVG
                     QNIL"
     gene            complement(10742..11740)
                     /locus_tag="VGT87_000275"
     CDS             complement(10742..11740)
                     /locus_tag="VGT87_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEQ3813167.1"
                     /translation="MPTFLELNTTISVKEKELLTKEQFDKLLQAPNTTTLARLLHQSV
                     YHLTVDDLNDLDRLESILMAELTKTYRWAFAETPQPDIVQLFTLRYTYHNVKVLLKAK
                     ASQADLSHLLLPIGDKPLVALEHLIRTMTSDEFPKEVVTEIQSIWAEYQDYQDIRVLE
                     IGTDLAYFKALKQIAQRLEDPVFQQAVLIVIDLYNLITVRRAKSQNKPISFMMQLLSD
                     EASRPSKTFITLEDDKDLMTWFENVTPDSYMTALKPYSEKLRQGTLQTTELEYLVDEC
                     LYHLFAKAKYQVDGPYVLARFLLAKSFEVKNLRLLAAALANDLPKERVIERMRPIA"
     gene            complement(11756..12340)
                     /locus_tag="VGT87_000276"
     CDS             complement(11756..12340)
                     /locus_tag="VGT87_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HEQ3813168.1"
                     /translation="MNDITQLRQNVLEKAHQEGQQCLKIATDSLDTDFKERQQQGLHD
                     LKAKRQKELKALEQQFQVAQQQLKNQERQALLALKQDSIKELFEASLEKMTNFSKEEE
                     LAFLKQVLSKYPEQPLQVTFGEKTGQKFSSYDCAELRLAFPQLSYNQELIPQEAGFLV
                     SLDQVDDNYLYRYLLESVLKEESSRIIDMLFSEI"
     gene            complement(12409..12888)
                     /locus_tag="VGT87_000277"
     CDS             complement(12409..12888)
                     /locus_tag="VGT87_000277"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEQ3813169.1"
                     /translation="MEHLAHYFTAHGGVFFAALGIVLAVALSGMGSAYGVGKGGQAAA
                     ALLKEEPEKFTSALILQLLPGSQGIYGFAIGILIWMKLTPELSVNQGLAYFLVSLPIA
                     IVGYFSAKHQGNVSVAGMQILAKRPKDFMKGVILAAMVETYAILAFVVSFILLNKIG"
     gene            complement(12890..14890)
                     /locus_tag="VGT87_000278"
     CDS             complement(12890..14890)
                     /locus_tag="VGT87_000278"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEQ3813170.1"
                     /translation="MAISQMKKLAMVFEKDYLDLVLKTLQQSQLVEVRDMKQLKHWQD
                     AFNKGNVKLPQIVQYDLTHQKPLLDDEALQYLLQSQQELENGLASLSAFLPPIGKLTA
                     LRQKTPSLSFKQFEESHRQQAAQTTLKMMSQKIERLEQLQNKIDQLTEYCQELEKWRS
                     LTVLPQDLAQFHFLSARVGTIPSTANDHFYHQLKQYKGLFIEEVYHTEFEYGLVLFWQ
                     AQDTIHLQKYQFKPLLYKEQLLPSEQLRINKELLTNWLAEKDSLLKELRQSQKILAQL
                     QVEIDYVLSQYQRQQTKKQLLGTRHLIALEGWIEVDSVNQLKGLMTETLGDMFYLDSY
                     DVTPDDWEDVPIKLRNHRYIAPFELVTEMYALPKYQEKDPTPFLAPLYLTFFGMMVAD
                     LGYGLLLYAVTLAALVFFNLQKTSKRLVTFFNILAISVALWGLIYGSFFGFDLPVALL
                     STKTDVITILVVSLLFGFVTLIFGLLLGAWQQVRMKAYATAYTSSLAWTFILLGLLLF
                     ILGKNVSGLAYLSVIGKWLALGNAFGILVVSLLKSKSLLGLGSGLYNLYGISSYLSDL
                     VSFTRLMALGLSGASIGAAFNMIVGIFPPVTRFTVGIFIFILLHAINIFLSMLSGYVH
                     GARLIFVEFFGKFYEGGGKAFNPLKLADNYVNVNEETDLEDN"
     gene            complement(14880..15200)
                     /locus_tag="VGT87_000279"
     CDS             complement(14880..15200)
                     /locus_tag="VGT87_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813171.1"
                     /translation="MNTLLSLMNSIEQEARTIYDSYDAKKKALADKKEEELKQLFLEY
                     EQETQLLLEAKREVFFEANRQEQKASKKRLATQQLALHAILEEHKLTVVRELVDKVVE
                     TYGH"
     gene            complement(15655..16674)
                     /locus_tag="VGT87_000280"
     CDS             complement(15655..16674)
                     /locus_tag="VGT87_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEQ3813172.1"
                     /translation="MDIIIGTSLLILVLAIFSLFNYKAPHGAKAMGALASAACASFLV
                     EAFQDSFFGKVLGFQFLSEVGGANGSLSGVAAAILVAIAIGVSPGYAVLIGLSVSGTG
                     IIPGFVAGYVVSFLIKWMEKNIPGGLDLISIIIVGAPLTRFLAQLITPVINSTLLTIG
                     DILTSSANSNPIIMGMILGGTIVVVATAPLSSMALTAMLGLTGIPMAIGALSVFGSSF
                     MNGVLFYRLKLGERKDNIAFAIEPLTQADVTSANPIPIYVTNFVGGGACGVLIALMKL
                     VNDTPGTATPIAGFAVMFAYNPVAKVLITALGCIIISLIVGYIGGSVFKNYRLVTKQE
                     LQARN"
     gene            complement(16711..17076)
                     /locus_tag="VGT87_000281"
     CDS             complement(16711..17076)
                     /locus_tag="VGT87_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEQ3813173.1"
                     /translation="MKSYPEPMGPYSTYTIEGHFLYTAGQLPLNPVTGQLSDGFEAQC
                     RQVFVNLQSILAEQKLDLNHIYKLNVYLTDVTNVEILNHVMTDLFEEPYPVRTAVQVS
                     ALPLQALIEVEAVARVKQL"
     gene            17250..17930
                     /locus_tag="VGT87_000282"
     CDS             17250..17930
                     /locus_tag="VGT87_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEQ3813174.1"
                     /translation="MDKETLNYWKTVITFLHDVLGDNYEIILHVIDKNDIYIGELVNS
                     HISGRSKQSPLTTFALDLITNKVYKEKDFVTNYKAIVSPQHKEVRGSTFFIKDKKGNL
                     EGMLCINLDISAYQGVARDLLKLVNLNLEHFIPTAKEPKTVTPQPEEAVEILTSNIQD
                     IIGQIIDPSLLRHNVHLSQDVKIDIVAKLYEKGVFQLKGAVSKVADILCISEPSVYRY
                     LKKIEADQ"
     gene            complement(18051..18698)
                     /locus_tag="VGT87_000283"
     CDS             complement(18051..18698)
                     /locus_tag="VGT87_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEQ3813175.1"
                     /translation="MAVALSKEEIQNRIAKRVAKELEDGTLVNLGIGLPTKVANFVPE
                     EMTVYFQSENGFIGLGPKSDDPNSTIVNAGGQPVTVYPGAAFFNSADSFGIIRGGHVD
                     LTVLGALEIAENGDIANYLIPGKMVPGMGGAMDLLVGAKKVIVAMEHTNKGKHKLLKE
                     CTLPLTAKGVVDLIITEMGVFKVTPDGIQVIEISEGFTFDEVQAATGVPLSIAEG"
     gene            complement(18701..19360)
                     /locus_tag="VGT87_000284"
     CDS             complement(18701..19360)
                     /locus_tag="VGT87_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEQ3813176.1"
                     /translation="MKENKRIAIAEAISHIKDGDTIMVGGFMANGTPEALIDALVDKG
                     TKDLILICNDAGFVDRGVGKMVANHQFKTIYATHIGLNKEAGRQMTAGETTIELIPQG
                     TFAEKIRIGAYGIGGFYTPTGVGTLVAEGKETKTIKGKTYLLEYPFEADVALIFANKA
                     DEMGNLQYSGSENNFNQLMAACAKTTIVQAREIVPVGTIQPECVHTPHIFVDYIVKEG
                     V"
     gene            complement(19372..20556)
                     /locus_tag="VGT87_000285"
     CDS             complement(19372..20556)
                     /locus_tag="VGT87_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEQ3813177.1"
                     /translation="MTKEVVITSAYRTPIGNFGGVFKSLSAVDLGVTVVTKILADTGL
                     KSDAIDEVIFGNVLHAGLGQNVARQVALNAGLSYDTPAFTIDMVCGSGLKAVELGAQK
                     IQTGNADIVLVGGTENMSQAPYVLQGQRWGSRMGDSKVVDTMLKDGLSDAFAGYHMGI
                     TAENIVQQYGLTREEQDAFAADSQRKAQLAIEKGRFKEEIAPVTIPQRKGEPLLVDQD
                     EYPKFGTTVDKLAKLRPAFIKDEGTVTAGNASGINDGAAAILLMSKEKAEELGLPILA
                     KITSYASAGVDPSIMGCGPIPATKKALAKAQLTIDDIDLIEANEAFAAQALAVSRDLG
                     FDNEKVNVNGGAIALGHPIGASGARILVTLLAEMAKRDVRHGLATLCIGGGQGQSIIV
                     TR"
     gene            20677..21587
                     /locus_tag="VGT87_000286"
                     /pseudo
     CDS             20677..21587
                     /locus_tag="VGT87_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993827.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(21657..23063)
                     /locus_tag="VGT87_000287"
     CDS             complement(21657..23063)
                     /locus_tag="VGT87_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acid transporter"
                     /protein_id="HEQ3813178.1"
                     /translation="MNNIKTKKRFMDRYIDGFMKWMPESLFICFILTFLVVTMSVLMT
                     DSPFIGTEKTGGIIYGWVNGFWGLLSFAMQMTILLATGNAVASSPPAHKMFKSLAKLP
                     QTRTQIFIFSIVVGSIFGFLHWGLGMMVAIVFGKELLVQARQKRIKVHTPLFVATLFF
                     TFLPATSGLSGAAVLYSATPDYLRNSVADAYKQVVPESVPLTESVLNLPFISLLVVCM
                     LVPLCFALLAHPKDETKIMELDDEIYHHSLDTASHVVIARNTPAEKMNASRLVMYLVG
                     GAIVSYSLYHFSVVGLSGLDLNSFNFLFLGLGLLLCGQQGPEYYGSLFKDGVMSSWGL
                     VLQFPFYAGIFGIIQSTGLGLEISHFFVAISNGTTWPVFAYLYSALLNIAVPSGGSKF
                     VIEAPYIVPATIEVGNDLGKILQAYQLGDATTNLIVPFWALSYLSNFKLKFNQIVAYT
                     IPCVLVVTGIAIIYLFVF"
     gene            23416..24081
                     /locus_tag="VGT87_000288"
     CDS             23416..24081
                     /locus_tag="VGT87_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813179.1"
                     /translation="MKRCNKYLFTSLLAASMLFSSYKSVHAHDNIDEKGKVHLYWQGN
                     YYVDNYVDYTKTLVDNDKNIEWTVTFNSAKEQWVYPDFSVFLPKGVKAPTEITYEHHY
                     WDGTVHSEKRRNTQWHYNWEKQKTNFNQEFDKFPGYTGWSPSLDKFYKLKNDGKFSHV
                     LVDTYGRQSHTYFSHKMVWKFKTELEEDYKNKWNKLPFIAGIKQNNPLAASFPSYKGE
                     FGE"
     gene            complement(24347..27847)
                     /locus_tag="VGT87_000289"
     CDS             complement(24347..27847)
                     /locus_tag="VGT87_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HEQ3813180.1"
                     /translation="MWREKINDTKKSYKLSFLLSLTGFILGLLLVFIGLSRVSVGHAE
                     TRNGANKQGAFEIKKVDQNNKPLSGATFSLTPKDGKGASVQTFESGSDGKIDAKDIKP
                     GTYTLKEDKAPDGYDKTSRTWTVTVYENGYTKLVENPYNGEIISRAGSKDVSQSLQLE
                     KSTMSVVSKYGEQEKTSNSADFYRNHAAYFKMSFELKQKDKSETINPGDTFVLQLDQR
                     LNPKGISQDIPKIIYDSANSPLAIGKYHAENHQLIYTFTDYIAGLDKVQLSAELSLFL
                     ENKKVLENTNISNFKSTIGGQEIKYGGTVNVIYGNEKNQKDNYVANGLSNVGGSIESY
                     NTQTGDFTWYVYVNPNRTNIPYATMNLWGFGRLTDKQAFSTDSNSSSAQLLNAEIYEV
                     PSDYHLPSSYGVDVTKLTLRTDITAGLGNGFQMTKRQRIDFKDNLKDGKRFVVKVTGK
                     TDQSGKPLVVQSNLASFNDQFAVKSSNPTNNVYFQNEIALSPSKGSGSGTSEFTKPSI
                     TVANLKRVAQLRFKKMSTDNVPLPEAAFELRSSTSDSQKVEAKSNEQGEVHFKDLTSG
                     TYDLYEKQAPKGYQQVTEKLATVTVDTTKPAEEIVKWKKTHPFVKVEVNKEVTIVNHK
                     ETLTFSGKKIWENDRPDQRPVKIQVQLLQNGQKMSDKVQEVTKDNDWSYHFKDLPKYD
                     DKNQEYKYSVEEVKVPDGYKVSYLGNDIFNTRETEFVFEQNNFNLEFGNAEIKGQSGS
                     KIIDEDTLTSFKGKKIWKNDTAENRPQAIQVQLYADGVAVEGQTKFISGSGNEWSFEF
                     KNLKKYNGTGNDIIYSVKEVTVPTGYDVTYSANDIINTKREVITQQGPNLEIEETLPL
                     ESGASGGTTTVEDSRPVDTLSGLSSEQGQSGDMTIEEDSATHIKFSKRDIDGKELAGA
                     TMELRDSSGKTISTWISDGQVKDFYLMPGKYTFVETAAPDGYEIATAITFTVNEQGQV
                     TVNGKATKGDAHIVMVDAYKPTKGSGQVIDIEEKLPDEQGHSGSTTEIEDSKSSDLII
                     GGQGEVVDTTEDTQRGMTGHSGSTTEIEDSKSSDVIIGGQGQVVETTEDTQTGMHGDS
                     GCKTEVEDTKLVQSFHFDNKEPESNSEIPKKDKPKSNTSLPATGEKQHNKFFWMVTSC
                     SLISSVFVISLKSKKRLSSC"
     gene            28212..29417
                     /locus_tag="VGT87_000290"
     CDS             28212..29417
                     /locus_tag="VGT87_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEQ3813181.1"
                     /translation="MVIFDLKHVQTLHSLSQLSISVMSQDKALIQVYGNDDYLLCYYQ
                     FLKHLAIPQAAQDVIFYEGLFEESFMIFPLCHYIIAIGPFYPYSLNKDYQEQLANNFL
                     KHSSHRSKEELLSYMALVPHFPINNVRNLLIAIDAFFDTQFETTCQQTIHQLLQHSKQ
                     MTADPDIIHRLKHISKASSQLPPVLEHLNHIMDLVKLGNPQLLKQEINRIPLSSITSS
                     SISALRAEKNLTVIYLTRLLEFSFVENTDVAKHYSLVKYYMALNEEASDLLKVLRIRC
                     AAIIHFSESLTNKSISDKRQMYNSVLHYVDSHLYSKLKVSDIAKRLYVSESHLRSVFK
                     KYSNVSLQHYILSTKIKEAQLLLKRGIPVGEVAKSLYFYDTTHFHKIFKKYTGISSKD
                     YLAKYRDNI"
     gene            complement(29560..30147)
                     /locus_tag="VGT87_000291"
     CDS             complement(29560..30147)
                     /locus_tag="VGT87_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEQ3813182.1"
                     /translation="MRKYWKMLFSVVMILTMLAFNQTVLAKDSTVQTSISVENVLERA
                     GDSTPFSIALESIDAMKTIEEITIAGSGKASFSPLTFTTVGQYTYRVYQKPSQNKDYQ
                     ADTTVFDVLVYVTYDEDGTLVAKVISRRAGDEEKSAITFKPKRLVKPIPPRQPDIPKT
                     PLPLAGEVKSLLGILSIVLLGLLVLLYVKKLKSRL"
     gene            complement(30164..30889)
                     /gene="srtB"
                     /locus_tag="VGT87_000292"
     CDS             complement(30164..30889)
                     /gene="srtB"
                     /locus_tag="VGT87_000292"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HEQ3813183.1"
                     /translation="MMMTIVQVINKAIDTLILIFCLVVLFLAGFGLWDSYHLYQQADA
                     SNFKKFKTAQQQPKFEDLLALNEDVIGWLNIPGTHIDYPLVQGKTNLEYINKAVDGSV
                     AMSGSLFLDTRNHNDFTDDYSLIYGHHMAGNAMFGEIPKFLKKDFFNKHNKAIIETKE
                     RKKLTVTIFACLKTDAFDQLVFNPNAITNQDQQRQLVDYISKRSKQFKPVKLKHHTKF
                     VAFSTCENFSTDNRVIVVGTIQE"
     gene            complement(30905..31948)
                     /locus_tag="VGT87_000293"
     CDS             complement(30905..31948)
                     /locus_tag="VGT87_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QVPTGV class sortase B protein-sorting
                     domain-containing protein"
                     /protein_id="HEQ3813184.1"
                     /translation="MKKNKLLLATAILATALGTASLNQNVKAETAGVVTGKSLQVTKT
                     MTYDDEEVLMPETAFTFTIEPDMTASGKEGDLDIKNGIVEGLDKQVTVKYKNTDKTSQ
                     KTKIAQFDFSKVKFPAIGVYRYMVSEKNDKKDGIRYDDKKWTVDVYVGNKANNEEGFE
                     VLYIVSKEGTSSTKKPIEFTNSIKTTSLKIEKQITGNAGDRKKSFNFTLTLQPSEYYK
                     TGSVVKIEQDGSKKDVTIGTPYKFTLGHGKSVMLSKLPIGINYYLSEDEANKDGYTTT
                     ATLKEQGKEKSSDFTLSTQNQKTDESADEIVVTNKRDTQVPTGVVGTLAPFAVLSIVA
                     IGGVIYITKRKKA"
     gene            complement(31970..32491)
                     /gene="lepB"
                     /locus_tag="VGT87_000294"
     CDS             complement(31970..32491)
                     /gene="lepB"
                     /locus_tag="VGT87_000294"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEQ3813185.1"
                     /translation="MTNYLNRLNENPLFKAFIRLVLKISIIGFLGYILFQYVFGVMIV
                     NTNQMSPAVSAGDGVLYYRLTDRYHINDVVVYEVDNTLKVGRIAAQAGDEVNFTQEGG
                     LLINGHPPEKEVPYLTYPHSSGPNFPYKVPTGTYFILNDYREERLDSRYYGALPINQI
                     KGKISTLLRVRGI"
     gene            complement(32484..34754)
                     /locus_tag="VGT87_000295"
     CDS             complement(32484..34754)
                     /locus_tag="VGT87_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus ancillary protein 1"
                     /protein_id="HEQ3813186.1"
                     /translation="MNNKKLQKKQDAPRVSNRKPKQLTVTLVGVFLMFLTLVSSMRGA
                     QSIFGEEKRIEEVSVPKIKSPDDAYPWYGYDSYDSSHPYYERFKVAHDLRVNLNGSKS
                     YQVYCFNINSHYPNRKNAFSKQWFKRVDGTGDVFTNYAQTPKIRGESLNNKLLSIMYN
                     AYPKNANGYMDKIEPLNAILVTQQAVWYYSDSSYGNIKTLWTSELKDGKIDFEQVKLM
                     REAYSKLISDDLEETSKNKLPQGSKLNIFVPQDKSVQNLLSAEYVPESPPAPGQSPEP
                     PVQTKKTSVIIRKYAEGDYSKLLEGATLRLTGEDILDFQEKVFQSNGTGEKIELSNGT
                     YTLTETSSPDGYKIAEPIKFRVVNKKVFIVQKDGSQVENPNKEVAEPYSVEAYSDMQD
                     SNYINPETFTPYGKFYYAKNKDKSSQVVYCFNADLHSPPESEDGGGTIDPDISTMKEV
                     KYTHTAGSDLFKYALRPRDTNPEDFLKHIKKVIEKGYNKKGDSYNGLTETQFRAATQL
                     AIYYFTDSTDLKTLKTYNNGKGYHGFESMDEKTLAVTKELINYAQDNSAPQLTNLDFF
                     VPNNSKYQSLIGTEYHPDDLVDVIRMEDKKQEVIPVTHSLTVKKTVVGELGDKTKGFQ
                     FELELKDKTGQPIVNTLKTNNQDLVAKDGKYSFNLKHGDTIRIEGLPTGYSYTLKETE
                     AKDYIVTVDNKVSQEAQSASENVTADKEVTFENRKDLVPPTGFTTDGGTYLWLLLLVP
                     FGLLVWFFGRKGLKND"
     gene            complement(35040..35888)
                     /locus_tag="VGT87_000296"
                     /pseudo
     CDS             complement(35040..35888)
                     /locus_tag="VGT87_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407229.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
     gene            complement(36176..>36851)
                     /locus_tag="VGT87_000297"
     CDS             complement(36176..>36851)
                     /locus_tag="VGT87_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HEQ3813187.1"
                     /translation="LEPALPPLMPELDGQEVPEKPSVDLPIEVPRYEFNNKDQSPLAG
                     ESGETEYITEVYGNQQNPVDIDKKLPNETGFSGNMVETEDTKEPEVLMGGQSESVEFT
                     KDTQTGMSGQTTPQVETEDTKEPGVLMGGQSESVEFTKDTQTGMSGFSETVTIIEDTR
                     PKLVFHFDNNEPKVEENREKPTKNITPILPATGDIGNVLAFLGILILSVLSIFSLLKN
                     KQNNKV"
BASE COUNT        11156 a         7724 c         6311 g        11660 t
ORIGIN      
        1 aaaaatgaac aaaaaaaaaa caagtagcca actgttacaa gtttcaaggg gatatttcca
       61 tcttattatc acagaacctt cacatctgtt ctatttaaac cagattaaaa gtactagtta
      121 aatatttgat agtagcaccg ttatcagtaa tagatctgtt aattatctct gattacaatg
      181 atgttaacaa tagtcatacc tttgtttgta cacacgcttc actgccaact aaaaaaggtc
      241 tgatggtaat atttatacca tcagaccttt gagagcctat agctctgcaa tttgataaag
      301 ttcaacttct gctactgttt cagagccaaa catgttgagc atcagcttca ctttgttgtt
      361 ttcgatttct accacacgac cttcttgtcc cataaaggca ccgtcgatga tctgaaccac
      421 atcgccttct ttgatattgg tatcaaagac atcaatggtt tgacccatag acaacaagat
      481 agcacggatt tcttcttcaa gaagtggtgt tggttttgat cggttcccgt gtgatcccac
      541 aaaacctgta acgtttggtg tgttacgcac aacaaaccaa gcttcatctg tcatgaccat
      601 ttcaaccaag acataccctg gaaagcgatt ttcttcgatt tctttggttt gcccattttt
      661 ttcaacgtta accgtttgtg tcggaatttc gacgcgcaaa atattgtcca acatgttata
      721 ggtttgggca cgttgcaaga ggttttcttt tactttgttt tcgtagcctg agtaagtttg
      781 gaggacgaac cagcccttat caaatgaatc tagcatgatg tttcctttca ttataaaaaa
      841 gccctttttt tggcttttgc gctgttttct atcaccttca ttataataaa agacagggcc
      901 gctgtcaacg tatctgtgga gctacttgac acctttatta aaaagtccaa agtcggttcg
      961 tgacgactct ggacggtatt gtacagtttt agttttcagg aactgtgatg gttccttcga
     1021 taatcgcttt tttagcagtc tcaatagctt ttttagtgtc ttgtgtcaga gcatccgttg
     1081 taagactaac accaccttct tttaaaccaa aagtggttat ttgaccacct gggaattggc
     1141 cttctgaggt ttttacggct acttttacta aagcttttcc gacttccttg atacttgagg
     1201 tcaaaacaaa attagctgac ttgccatctt ttgtggtgta ttttccatct tcactttggt
     1261 cacggtcaac accaataacc caaaccttat cttcttcttt acgtttttcg ttgatagact
     1321 tagcttcgct aaagacaccc gcccctgtgc ctcctgctgc atgataaata acatctgcgc
     1381 cttcaacgta ttgagcagct gcaatcgtct tgcctttggc agcatctgca aaagagcctg
     1441 cataagcaac tcttactttg atggtatcat ctactgattt cacaccagct tcaaaacctt
     1501 tttcaaagcg cttgacaaca tctccttcca taccacctac aaaaccaact tgcttggttt
     1561 ttgtcgtttt agctgctgca acaccggcta ggtatgccgc ttcatggtct gaaaaggtga
     1621 tacttgcaac atttttttga cctttaacca catcatccac aattgcaaaa tggttgtcag
     1681 gattgtttgc ggctactttt tctacagcgt catgtaatgg aaatccaatc ccaaaaacaa
     1741 gattataacc attggttact gctgactcaa ggtttgtggt aaactctgat tcattggctg
     1801 attggaaata atcatagcct tttccttttt caagtttatt ttcttttccc caagcttgta
     1861 agccttccca ggctgactgg ttaaatgact tgtcatcaat acccgtctga ttagtaatca
     1921 tagcgatttt taatgaattg tcagttgttt gtttagcaga gttattggta cagcctccta
     1981 atgtgaatag ggcagtcgaa acaagaccaa gtgacattac ttttttgttc atgtgaaaat
     2041 gaacctccta aatgtttaat cctaaagtga tgtgtcattg ttaacgttct acttatgctc
     2101 tagaacgaaa agtttagtat atcaaaaaaa tcagttttat cctactattt ttgctgaaaa
     2161 atctctttct caccaaaatg accgaacgat aaagtgagat caactggtaa aacccattat
     2221 ctcctcaaaa atcacctctt aaggagataa gacaacgaca aaactcaagc aactagtaat
     2281 agcatgacta actatacaat ccaacaaacg gtaaaaagag ctggaaacgc tatccaactc
     2341 tttgtcatac tagacacctt agatagatgc ccagactgac tcaagaatat tggtctgttc
     2401 acgccctgga ccaactgaga aggttgaaat gcgaacgcca accaattctc caacacgacg
     2461 aacgtagttg cgggcatttt caggaagctc atcaaggcta cgaacacctg tgatgtcctc
     2521 ttgccagcct ggtaattctt catagattgg tttgcaacgt ttgagttgtt caaggcttgc
     2581 tgggtaatag tcaatacgtt tcccatcaag gtcataagcc acacaaatct taaccgtatc
     2641 aagccctgaa aggacatcaa ttgaattcag agagaggtta gtaatccctg acacacgacg
     2701 actatgacgc ataacaactg agtcaaacca acccacgcga cgtggacggc cggtcgttgt
     2761 cccgtactca tgacccactt cacggatacg ttcacccact tcatcaaaga gttctgtagg
     2821 gaatggccca tcaccgacac ggcttgtgta ggctttacag acaccaacta ctttgttgat
     2881 tttacttggg ccaacaccag aaccaatggt tacaccacca gcaactgggt ttgaagacgt
     2941 tacaaatggg tacgttcctt ggtcaatatc aagcataact ccttgagcac cttcaaaaag
     3001 aacacgttta ccggcatcaa gtgcatcatt taaaatcaca gaagtatctg tcacatattg
     3061 cttgatttct tgaccgtaag catagtattc ttcaaaaata gcatcaaaat caagaggtgt
     3121 actgtcatac attttttcaa ataaacggtt tttctcagct aaattaatgc gtaggcgctc
     3181 tgcaaagata tccttatcca gtaaatctgc gatacggata ccaactcgag cagctttatc
     3241 catataagct ggaccaatcc ctttaatagt tgttccgatt ttattgtcac cttttgcatc
     3301 ttcttgcagt tgatccagct ggatatgata aggtaaaatg acatgagctc ggtctgaaat
     3361 gcgaaggtta tctgtcgtca ctccttcatc atgcaggtac gccaactctt tgaccaaaga
     3421 tttggggttg accacaacac cattaccaat aacggaaatt ttttgcggga agaaaattcc
     3481 tgatgggatc aagtgaagtt taaacttctt accatcaatc acgatcgtat gaccagcatt
     3541 atcaccacct tgatagcgag caattacttc tgcatccgct gacaagaaat cagtgatttt
     3601 acctttacct tcgtcacccc actgggtacc aacaacaact acagatgtca tatcattgtc
     3661 ctaactttct gaaacactta ttctcaataa aaatcatagg caagctaacc actgcttaaa
     3721 aaccaaaaag aaggtcagat ctgtaagatc agcaatgtag ctcttgatat ttaaagaaaa
     3781 caggcaggaa tctcacctgc tagcatatat cttacaactt attatagcaa aaaatggttt
     3841 tttctgctag atttcttagc attattaagg aaaaagagaa actctttttt taaccatgat
     3901 aaacaatcct aaaaaaacga acattataag agaaaagctt ttaacctcgt ttctcctaag
     3961 ctttagcagg gttaaacgat tctcttaaaa cattagctct tttctttcag aaatagcggt
     4021 tttaaggtaa aataatagta agttacctct atgagatagc cagtaaaaac atatcgagga
     4081 aatatcacat gattagaaag tatgaccgca cgagcacaaa aaagaaatca ctaaactgga
     4141 tttggctaat cattgctttt tttatgattt caagctttat aggtggctct tcttttactg
     4201 agtcacttct agacattttg cctgccatag ccattggtgg tacaggttat gctatttttc
     4261 gtgttagatc tcatcaaaag cggctagcaa aggccaaggt tgccaaacaa ttagaagatc
     4321 taaaagcaaa aattcagcta gctgatcgta aagttcggct gttagatact tatttggcag
     4381 accatgatga cttccaatat aacgtactag ctcagcaatt attaccacaa cttagtgaca
     4441 tcaaagcaaa agctattacg ttaaaagacc agttagatcc caaaatttat cgtcgtatca
     4501 ccaaaaaagc caatgacgtt gaaagtgata ttacccttca actcgaaaca ctgcagattg
     4561 ccacaactct taaccctcag cctttgaaaa ccccatctcc taatcttatt aacaaagcac
     4621 ctgagctcaa accgtattat gataatattc agacagatca tcaagctatc cttgctaaaa
     4681 tccaaggagc cgacaatcaa gaagaattgc ttgcgctgca tgaagctaac atgaggaggt
     4741 ttgaggatat cttaacaggc tacttaaaaa tcaaagaaga gcctaaaaat tactataacg
     4801 ctgctgctcg tttagaacaa gccaaacaag ctatccaaca atttgatgaa gatcttgacg
     4861 aaacccttcg tcgactcaat gaaagtgatc ttaaagattt tgatattagc cttcgaatca
     4921 tgcaaggtgc tactcaaaga cgaacaacgc atcaccaaaa agactaaagg agtcccttta
     4981 tggcagattt taattttgac attgaccaaa ttgctgacaa tgctgttgtc aaaaccgata
     5041 aaaccaccga tattatctca gacttaccaa ctgacacaaa tggccaaatc tctttttttg
     5101 agaaactaag tgctgaccag cagacagcta ttactgccaa agccccggca ttggtagata
     5161 cctttttagc agatcaaaat gcgctacttg attttggtca gtctgctgtt gaaggggtca
     5221 atgcaacggt caatcatatc ctggccgaac aaaagaagtt acagattcct caggttgatg
     5281 atcttttaaa atcaaccaat cgtgaactca atggttttat cgctaaatac aaggatgcca
     5341 cacctgtgga ccttgataaa aagccaaact ttcttcaaaa actctttaaa caaagccgtg
     5401 ataccctgca agagttttat tttgattctc aaaacattga gcaaaaaatg gataatatgg
     5461 ctgcagcggt tgtcaagcaa gaagatacct tagctcgcaa tattgtctca gcagaattgt
     5521 taattgaaga taatactaag tccattgaac atttggtagg cgtcattgct tttatcgaag
     5581 ctagtcaaaa agaagctagt cagcgcgctg cggctttaca aaaagaccta aaaaccaaag
     5641 acagtactac accagactat caaataaaag ctgatctttt ggcaagaact accgaagtca
     5701 tcaacaccct cgaacaacaa cacaccgaat acttgagtcg cctctatgtt gcttgggcga
     5761 caactccgca aatgcgtaat ctagtcaagg tctcttctga catgcgtcaa aaactaggca
     5821 tgttacgccg caataccatt ccaacgatga aactatctat tgctcagtta ggcatgatgc
     5881 aacaatcggt taaatctgga atgactgctg acgccattgt caatgctaat aatgctgccc
     5941 ttcagatgct agcagaaaca agtaaagaag ccatcccagc attggaacaa tcagcacaaa
     6001 gccctacttt gtctatgaaa tctgtgacaa gtctggcaga aagtttagta gcacaaaata
     6061 atggcatcat tgcggcgatt gatcatggac gcaaagaacg agcacaacta gaaagtgcta
     6121 ttatcaggtc tgccgaaacc attaacgatt ctgtcaaact acgtgaccaa aatattgtgc
     6181 aagcacttct tagtgaaggc aaggaaactc aaaaaacaat tgataaaaca actcacgcat
     6241 aagctaaaca tcacctgtca cgctacttaa aaagcaagcc atcagttagg ttaatccctt
     6301 tgatggcttg cttttttact acttaacgaa taatcgcctt gtttgatata acacttatct
     6361 tttatgatgt tattttcata atacgaacaa ggctagcacg ctcggcctct tctaatttca
     6421 actcaatgta gtgaatcgtc tcttccagtt gcgggataat agaatactct aagccattga
     6481 cgcgacgtct agtcttttca atgtcatcag ccattagttg acaagttttt tcaacttcag
     6541 ctaaccgtaa gagcttttct aatagttctt ttgtcttttg gatagtattg tccatctcac
     6601 tatttgagct gaggtaacta taagcaaact caccttgttc ttgctctctt gccgtattag
     6661 actggacatg gaatttaggc acattgacac tcatgatatt ttcaatatca atccataagg
     6721 tcacttcatg gactggaaca gcaaagagtt cttctaccat tagactattt tcagatgctt
     6781 ttgccaagac aaactccttc atattggcag caagttcttt ttcgagagtt tgacgaagct
     6841 cattgttttc acgaattaaa tcaacgaagc ggcgcatcaa ctcatcccgc ttgtccttta
     6901 ataatttatg acctcttgta gctgtcttca atcgattttt caagttacta agttccatgc
     6961 gtgttggttt aacatttaat cgtgccattg atataactcc tttcgtcaag ggcttgaaat
     7021 tgcatggtca actcaatcat ttgcaacaaa aacctttgtc atcgtggtat ctgctttagg
     7081 caaataacga tctaacatat catctttaat gcgttttaat tcggtacgtg gcaaaatcga
     7141 caacaattcc caacccaagt caagactttc ttcaatgcta cggttggtgt aaaatccttg
     7201 gttaatgtat tcttcttcaa accggttggt aaaggccaca tatagtttat ccgtttcaga
     7261 aagggctgat tcaccgagca caacagccaa ttccttagct tgttttcctt gggcataggc
     7321 tgcaaagagc tgattcattg tggcagcatg gtcttgtcga gtcttacctt cacctgaacc
     7381 tttatctttt aggcgagata gcgatggcaa gacattaata ggcggtctaa aaccattttt
     7441 atatagttct tgcgacaaaa taatctgacc ttcggtaata taacctgtca aatcggggat
     7501 agggtgggta atgtcatctt ctggcatggt taaaatagga atttgagtca ctgacccttt
     7561 tttcccgatc aagcgaccag cccgttcata taaggtggag agattagtat agagatagcc
     7621 aggataacct cgtctgcctg gaacttctct gcgagcggct gatacttctc gtaaggcttc
     7681 acaataattt gtcatatctg tcatgatgac taaaacatgc ataccttttt cataagccaa
     7741 gtattcggct gttgtcagag ctatacgtgg tgttgcaatg cgttcaatag cagggtcatt
     7801 agctaaatta ataaataaaa cagatcgatc aatggcaccc gtttcacgca agtcattcat
     7861 aaagaattct gcctcttcaa aagtaatacc catagctgca aacacaaccg caaagttatc
     7921 atcagagttt agaacagttg cctgacgagc aatctgtgcg gccaattcat tatgaggtaa
     7981 ccctgagccc gaaaaaactg gtaacttttg accacgaacg agggtattaa ggtgatcaat
     8041 agctgagata cctgtctgga taaattcgtc tgggtaatca cgagcgacag gattgatcgc
     8101 ttgaccatca atatcaaggt atttttcggg aataagttct ggaccgccgt ctatgggctg
     8161 ccccattcca tcaaagatgc gccctaccat atcttctgat acggctaatt ctaaaggatg
     8221 ccctgtaaat cgaaccttgg ttttggctag gttgatgcca gttgatcctt caaatagctg
     8281 taccatagcc ttgtcacctt ggacttctaa gacttgacct ttacgccttt ctccattatg
     8341 caaagtaata tctaccaatt cgttgtagtg cactcctgca acttgatcaa caatcatcaa
     8401 agggccaacc acttcgctaa ccgtccgata ttctttgaga acgctcatct aaattcctcc
     8461 ctttgctaaa acgtgatgaa tctctttggt aaccttctga gtaagccctt taatctgctc
     8521 taagttttct tctgggataa atttgctgcg tgcgatgcga tcacgtacct gagcagtgcc
     8581 ttccataatc tcattaaaat aagctcccaa agaaagggct ttgctggctt cctcattaaa
     8641 ggtcaaaata ttggttagca tggcctcttg tttcggaaag gaagtaaagg tatccaccga
     8701 atcaaaggca ttttgctgga gataatcctc ccgaatttgc cgggcaacag ccatggtcaa
     8761 acgatcttgt tcagacagtg aatcaagccc caccaagcgt acaatttctt ctagactggc
     8821 ttcacgctgc aaaatagcca tggcacgagt taccttgttg gaccaattag attgctgttt
     8881 acggtcaata tagcttccta catcatcttg atacaatgaa taagacgtca gccagttaat
     8941 cgctgggaag tgacgccgtt gcgcaagagg cgcgtcgagc ccccagaaaa ctttgacaat
     9001 ccgaagggtg ttttgagtga caggctctga aatatcccct ccaggaggag aaaccgcgcc
     9061 gatggctgta atggttccct cacgttcttg acttcccaag gtccgaacac gaccagcccg
     9121 ttcataatat tcggcaatac gactccctaa gtaagccggg tagccttcat caccaggcat
     9181 ttcttgtagg cgtcctgaca tctcgcgcag agcttctgcc caacgtgatg tcgagtctgc
     9241 catgatagcc acagaatagc ccatatcacg gaaatattcg gcaatggtaa tacctgtgta
     9301 aatcgacgct tcacgcgctg ctactggcat attagaggtg tttgcaatta acaccgtgcg
     9361 ctccataatg gactggcctg tatttgggtc aattaactct ggaaactcat tcaaaacgtc
     9421 ggtcatctcg ttgccgcgtt ccccacaacc gacataaata acaatatcaa cgttggcaaa
     9481 tttagctatt tgatgctgca caactgtttt tcctgcccca aatggtccag gaacggcagc
     9541 ggcaccacct tttgtaacag ggaaaaaggt gtcaataacc cgttgacctg taaccaaagg
     9601 ttcgactggg ataagctttt gagcaacagg gcgactttga cgaactggcc aagtctgcat
     9661 gaggctacct tggtaaatgg aaccgtcttc ctgcttaatt tcataaactg tatctgttac
     9721 tgtgaagtcc cctgtatgaa tagccaccaa ggtccctgaa actttataag gaaccatgat
     9781 acggtgctca atgacagctg tttcttgcac agttcctaaa atatcacctg caactacttc
     9841 ttgaccaaca cttagcttgg gaataaatgc ccacttagcc tttcgatcaa gacttgggat
     9901 agccacacca cggattaaaa agtcgctgtc cgttgctttt tgaaaacgat caagcggtcg
     9961 ctgaataccg tcaaacattt ctgaaatcag gcctgggcct aactcgaccg acaaaggaca
    10021 accagtagtc actactggtt ctcctggacc gatccctgat gtttcctcat aaacctgaat
    10081 agacgcttga tcgcggcgca tttcaataat ttctccgact aagccaagat gtcccacacg
    10141 acaaatatct tgaatattag cttcttgcat cccagaagcc acaacaagag gtcccgaaac
    10201 ggttattatt tttccttggt tcaccttttt cctcctttat ttttttcaaa tcatactaac
    10261 taatctatac tgtcaaggtt taatgtgact attttgaata gtcagcaaac tatcctatgc
    10321 gttcagctgc taacttcacc aattttcctg tccttgcatt tcatcagtta gctactcata
    10381 agcctcaaac agagtataag tcctctttgc ataacgtctt agtgttataa aatattttga
    10441 ccgacagcct tttcaaccat ggcttgtatg cgtgacaggc ctatcccttg cgctccttga
    10501 tgagttggta atggtaggac tgctggtaga acttgattgt catagtgagt gagggcttct
    10561 ggaatagctg cagcaatatc ttctgtgata taaataatac caaagtcttc cattgctagt
    10621 tgacgcaact ggttaatagc gtcttgtggt tttattactg gaaaagtttg aaacccaatc
    10681 atctgaaaag gtagaataac atctctgtta ccaattaccc cgactttata caatgttttg
    10741 gtcatgctat tggtctcatc ctttctatga cacgttcttt gggtaaatca ttagctaaag
    10801 cagccgctaa aagccgtagg ttttttactt caaacgattt agcgagcaaa aagcgagcaa
    10861 gcacataagg tccatcgact tgatatttgg ccttcgcaaa taaatggtaa aggcattcat
    10921 caacaaggta ttctaactca gtcgtttgaa gagtgccttg gcgaagtttc tctgaataag
    10981 gttttagggc tgtcatatag ctatcaggtg ttacattttc aaaccacgtc atcagatcct
    11041 tatcatcttc aagtgtgata aatgttttac ttggcctact tgcttcatca gataataact
    11101 gcatcatgaa acttattggt ttattttgcg acttggcccg tctgacggta atcagattat
    11161 acaaatcaat cacaataaga acagcttgtt ggaaaacagg gtcctctaat ctttgtgcta
    11221 tttgtttcaa cgccttaaaa taagctaagt cagtaccaat ttctaagacg cgtatgtctt
    11281 gataatcttg atattcggcc caaatggact ggatttctgt tactacctct tttggaaact
    11341 catcagaagt catcgtccta atcaaatgtt ccaaagccac cagaggcttg tcaccaattg
    11401 gtagtaataa gtgactcaga tctgcttgac tagctttagc ttttaacaac actttgacat
    11461 tatgataggt gtaacgcaag gtaaataatt gtactatgtc aggttgaggg gtttctgcaa
    11521 aagcccagcg ataggtcttg gttagctcag ccattaagat agattctagc cgatccaaat
    11581 cgttgagatc gtcaacagtt aggtgataga ctgactggtg caacagtcga gctaaggttg
    11641 ttgtattggg agcctgcaat agcttatcaa attgctcttt agtcaataac tctttttctt
    11701 tgacactgat agtagtgttt aattctagaa atgttggcat cgctagtcaa acctcctata
    11761 tctctgaaaa aagcatatca atgatgcggg agctttcttc tttaagaact gattctaaaa
    11821 ggtaacggta taaataattg tcatcgactt ggtctaaact gactaaaaat cctgcttcct
    11881 gaggaattag ctcttgatta tagcttaatt gaggaaacgc taatctcagc tcagcacaat
    11941 cataagaact gaacttttgc cctgtttttt cgccaaaagt aacttgcaat ggctgttctg
    12001 gatatttaga caagacttgc ttcaaaaaag ccaattcttc ttccttagaa aagttcgtca
    12061 tcttctccaa agaagcttca aatagctcct ttatactgtc ttgttttaat gctagtagag
    12121 cttgtctttc ttgatttttg agttgctgct gcgcaacctg aaattgctgt tcaagagctt
    12181 ttaactcttt ttgacgcttt gcttttaaat cgtgtagtcc ttgctgttga cgctctttaa
    12241 agtcagtatc aagactatct gtcgctatct ttaggcactg ttgaccttct tgatgtgctt
    12301 tttcaagaac gttttgtctt aactgtgtta tatcattcac ctaatttccc ccttttgata
    12361 gtgttagtgg tgtcaccgtt ttttaaaatc tagataagga ccgttacctt atccaatttt
    12421 atttaagaga ataaaggaga cgacaaaggc aagaatagca taggtttcaa ccatagctgc
    12481 taaaatgaca cccttcataa aatctttagg gcgcttagcc aagatttgca taccagctac
    12541 agaaacattg ccttggtgct tggccgaaaa ataaccaaca atagcaattg gaagagatac
    12601 caaaaagtaa gctaatcctt ggttaactga aagttctggt gtcagtttca tccaaattaa
    12661 aataccaata gcaaagccgt agattccttg actaccgggt aacagttgca agataagcgc
    12721 agacgtaaat ttttcgggtt cttcttttaa gagcgcagct gccgcttgac cacctttacc
    12781 gacaccataa gctgagccca tgccactaag agctacggct aaaacaattc ctaaggctgc
    12841 gaagaaaacg ccaccatgtg ctgtaaaata atgtgctaaa tgttccatgt taattgtcct
    12901 ccaaatctgt ttcttcattg acattgacat aattatcagc taactttaaa ggattgaaag
    12961 cttttcctcc accttcataa aattttccaa aaaactctac gaaaataaga cgagcaccat
    13021 gcacatatcc tgaaagcatt gataagaaaa tattgatcgc atgcaaaaga ataaaaataa
    13081 agatacctac tgtaaagcgc gtgactggtg gaaaaatccc aacaatcatg ttaaaagccg
    13141 ctccaatact agctccagaa agccctaatg ccattagacg agtaaagcta accaaatcac
    13201 ttaagtaaga gctaatgcca tataaattat ataatccact acctagacct aaaaggcttt
    13261 tgctttttag taaagaaaca accaaaatcc caaaggcatt gcccaatgca agccacttac
    13321 caataaccga taagtaagca aggccagata cattcttacc aagaataaat aacagcaggc
    13381 ctaataaaat aaaagtccaa gctagacttg acgtataggc cgtagcatat gctttcatcc
    13441 ttacttgttg ccatgctcct aacaaaagcc caaatataag tgtaacaaac ccaaatagca
    13501 aagaaacaac caaaatagta atcacatccg ttttagttga taataatgcc acaggtaagt
    13561 caaaaccaaa gaaagaaccg taaataagcc cccatagggc tactgaaata gctaaaatat
    13621 taaagaaagt cactaaccgt ttactggttt tttggagatt aaaaaagacc aatgcagcta
    13681 gtgtcacagc atatagcaac aaaccatagc ccaaatcagc taccatcatg ccaaaaaaag
    13741 taaggtataa aggagctaaa aaaggtgtcg gatctttttc ttgatatttt ggcaaagcgt
    13801 acatttctgt aaccaattca aacggtgcaa tgtagcggtg attacgtaac ttaatgggaa
    13861 catcttccca atcgtctggc gtgacatcgt aactatccag ataaaacata tcacctagtg
    13921 tctcagtcat tagccctttt aattggttaa ccgaatcaac ttctatccac ccttctaagg
    13981 ctattagatg tcttgtaccg agtaattgct ttttagtttg ctgccgttga tactgactaa
    14041 ggacatagtc tatttcaacc tgtaactgtg ccagtatctt ttgagactgc ctaagctctt
    14101 ttagtagact atctttttca gctagccaat tcgttagtaa ctctttgttt atacgaagtt
    14161 gttctgatgg cagtagctgt tctttatata ataagggttt aaactgatat ttctgtaaat
    14221 gaatcgtatc ttgtgcttgc caaaataaga caaggccgta ctcaaattct gtatggtata
    14281 cttcctcaat aaaaagacct ttgtactgct ttagctgatg ataaaaatga tcatttgctg
    14341 tactaggaat cgttccaact cttgccgaca aaaaatgaaa ttgcgctaag tcctgaggca
    14401 aaacagtcaa ggaacgccac ttttctaact cctgacagta ttctgttagc tggtctattt
    14461 tgttttgcag ctgctctagt cgctctatct tttgtgacat cattttcaaa gtcgtttgag
    14521 ctgcttgttg tcgatggctt tcttcgaact gtttaaacga aagagatggc gttttttgtc
    14581 tcaaggctgt taactttcct attggtggta aaaacgctga taagctagca aggccatttt
    14641 ctaactcttg ttggctttgt aaaaggtatt gtaaagcttc atcatctagt agaggttttt
    14701 gatgcgtcag atcgtattgg acgatttgtg gcaacttgac gttgcctttg ttaaaagcat
    14761 cttgccaatg ttttagttgc ttcatgtcac gaacttcaac taattgagat tgttgaagtg
    14821 tttttaaaac aagatcaaga taatcttttt caaaaaccat tgccagcttt ttcatttgac
    14881 taatggccat atgtctccac caccttatcc actaactccc taacaacggt tagcttatgc
    14941 tcttctaaaa tggcatgaag ggctagctgt tgcgtcgcta acctcttttt tgaagctttt
    15001 tgttcttgac gattagcttc aaaaaacacc tcacgtttag cttctaatag tagctgagtt
    15061 tcttgttcgt actcaaggaa cagctgcttt aactcttctt cttttttgtc agctagagct
    15121 tttttcttag catcgtaact gtcataaatt gttctagcct cttgttcgat actattcatt
    15181 agtgacaata gggtattcat cacatatcaa ctccttatct ttatctattg ctaattcttt
    15241 gactcactag gtacttaaaa acaaagctac aagaaaggct acttttgggg ggttaatcaa
    15301 ccagcgaata taagctagag ctaaatctct aacctatcta ctaacagctt ttttgagata
    15361 ggataatagc ttaattaaac gaacagcatc tgtaagttaa gcgctttcaa ttctatccat
    15421 aaaaacaatg gcttagttca gaaaaaggtt agctaaaaac ctgtcaaagc agtaagatga
    15481 cacagaactc taaagctttg tattgctgtc tttcatgtgt tatcttatac ttctttaggc
    15541 atttagtaac cttgatctcg ttgacggtaa ggaaccttta ccagccaata gccaagccaa
    15601 tgtgataaca gtataactgc tatcgtgtta aaagtcctgt caaaaagatg tttattagtt
    15661 tctcgcttgt aattcttgtt ttgttactaa acggtaattt ttaaagacag aacctccaat
    15721 ataacccaca attaagctga taataataca tccaagggct gttatcaaca ctttagcgac
    15781 aggattatag gcaaacatca ccgcaaaacc ggcaatcgga gtagctgtcc ctggcgtatc
    15841 attaaccaat ttcattaggg cgatcaaaac accacatgca ccaccaccaa caaaattagt
    15901 cacataaatc ggaatgggat tggctgacgt cacatctgcc tgagtcaacg gttcaatagc
    15961 aaaagcaata ttgtcttttc tttcacccaa ttttaatcgg taaaaaagaa caccattcat
    16021 aaaagaagag ccaaagacag ataaggcacc tatagccatc ggaatacctg ttagaccaag
    16081 catggctgtc aaagccattg atgatagcgg agctgttgct acaacaacaa tcgtaccgcc
    16141 tagtatcatt cccatgataa tagggttgct attagcactt gaggtcagga tgtctccaat
    16201 agtcaataaa gtactattga tcactggcgt aattaactgc gccaagaaac gagttaatgg
    16261 tgcaccgaca ataataatcg agattaaatc aagcccacca ggaatatttt tttccatcca
    16321 cttgattaaa aaagatacca catacccagc cacaaaacct gggataatac ctgttcctga
    16381 aactgacaaa ccaatcaaaa cggcataacc tggagacaca ccaattgcaa tggcaaccaa
    16441 gatagcagca gctacccctg ataatgagcc atttgctccg cctacttcac ttagaaactg
    16501 aaagcctaaa accttaccaa aaaaagaatc ttgaaaggct tcaactaaaa aagaggcaca
    16561 agctgccgaa gccaaagctc ccattgcctt ggcaccatga ggagccttat agttaaacaa
    16621 gctaaaaata gctaaaacaa gaatcaaaag acttgttcca ataataatat ccataatcta
    16681 ttccaccaat ctatctcatt acataagaca ctataactgc ttcactctgg caacggcttc
    16741 gacttcaatc aaagcttgca aaggaagcgc cgacacttga accgctgtcc gaacagggta
    16801 aggctcttca aacaaatcag tcatgacatg atttaatatt tccacattgg tcacatctgt
    16861 taaatacaca tttagcttat agatatgatt caagtccaac ttttgctctg ccaaaataga
    16921 ttgtaaatta acaaaaacct ggcgacactg tgcctcaaaa ccatctgaca attgacctgt
    16981 cactggatta aggggtaatt ggcctgcggt gtatagaaaa tgtccttcaa tcgtataagt
    17041 tgaataaggg cccattggtt ctgggtagct cttcatatac gaacctcctc attgataata
    17101 tagttaaatt ttacaggaaa ccgcattcac agtcaataat aaattctctt tttgataaaa
    17161 aattattatt aaggtgattg gcaattgttt tttagtctaa aatgtattac tatatttgta
    17221 aataagaaga tagatatagg aagtttgaca tggataaaga aacgctaaac tactggaaaa
    17281 cggttatcac atttcttcac gatgtcttag gtgataacta tgaaattatt ttacatgtga
    17341 ttgataaaaa tgatatctat attggagaac tcgttaatag tcatatcagt ggacggtcta
    17401 aacagtctcc tctcaccacc tttgctcttg atttgattac caataaagtc tataaggaaa
    17461 aagattttgt gaccaattac aaagcaatcg ttagtcctca gcacaaagaa gttcggggat
    17521 ctactttttt tattaaagat aaaaagggta atctcgaggg aatgctatgt attaacttag
    17581 atatttcagc ctatcaagga gtagctaggg acctcttaaa acttgtcaat ctcaatttag
    17641 aacatttcat cccaactgct aaagaaccta aaacagtcac accccagcca gaagaagctg
    17701 ttgaaatttt aacctctaac atccaagata ttattggtca aattattgat ccctcgttac
    17761 tgagacacaa tgtacatctt agccaagatg tcaaaataga cattgttgcc aagctttatg
    17821 aaaaaggagt ctttcaacta aaaggagccg tctcaaaagt cgctgatatt ctatgtattt
    17881 ccgaaccaag tgtttaccgc taccttaaaa aaattgaagc cgatcaataa ccaaatcacc
    17941 ataaaacgcc agttaaaagt atttcagata agcatcacaa tctctgatat accgatgact
    18001 gacgtttttt tactcgatat gcctttatgg caagaaaagg ctactagtca ttaaccttca
    18061 gctatgctta gaggtacgcc tgtcgcagct tgaacctcat caaaagtaaa cccttcactg
    18121 atttcaatta cttggatacc atctggcgtt actttaaaca cacccatttc tgtgataata
    18181 aggtcaacca caccttttgc tgttaaaggt aaggtacatt cttttaggag cttatgcttt
    18241 cctttattgg tatgctccat agctacgatg acttttttag caccaactaa taaatccatc
    18301 gctcctccca taccaggaac cattttgcct ggaatcaggt aattggcgat atctccattt
    18361 tctgcaattt ctaaagcacc caagacggtc aagtcaacat gaccaccacg tataatgcca
    18421 aaagaatctg cactattaaa gaaagcggct cctggataaa ccgtcacagg ctgtcctcct
    18481 gcattaacga tagtcgaatt cggatcatct gacttaggac ctagtccaat aaacccattt
    18541 tcagattgaa aatacactgt catttcttca ggcacaaaat ttgctacttt ggttggtaga
    18601 ccaataccta aatttaccaa agtaccatct tctaattctt tggctacacg ctttgcaata
    18661 cgattttgaa tctcttcttt acttaatgca accgccataa ctatactcct tctttaacaa
    18721 tgtaatctac aaaaatgtgt ggtgtatgaa cgcattctgg ttgtatggtg ccgactggta
    18781 ctatttctcg cgcctgaaca attgttgttt ttgcacaagc tgccatcaat tgattaaaat
    18841 tattttcaga accactatac tgtaaatttc ccatttcatc agctttatta gcaaatatca
    18901 aggcgacatc tgcttcaaat gggtattcta agagataggt ttttcccttg atggtttttg
    18961 tttccttgcc ttctgccact aaggttccta cccctgttgg cgtgtaaaag ccaccaatgc
    19021 cataagctcc gatgcgaatt ttttcggcga aggttccttg aggaatgagc tcaatcgtcg
    19081 tttcaccggc agtcatttgg cgcccagctt ctttatttaa tccaatatgt gttgcataaa
    19141 tggttttgaa ttgatgatta gccaccattt ttccaacacc gcgatcaaca aaaccagcat
    19201 cattacagat caagattaag tcttttgtgc ctttgtcaac cagtgcatca attaacgctt
    19261 ctggtgttcc attagccata aaaccaccga ccataatcgt gtcaccatct ttaatatgac
    19321 tgatagcttc tgctattgct attcttttat tctctttcat acggttacct tctatcttgt
    19381 tacgatgata ctttgacctt ggccaccacc aatacaaagc gttgctaagc catgtctcac
    19441 atcacgctta gccatttctg ctaataaggt gactaaaata cgagcacctg atgctccaat
    19501 cggatgaccc aaagcaatag ctcctccatt gacattaact ttttcattgt caaaaccaag
    19561 gtctcgtgaa accgctaaag cctgagcagc aaaagcttcg tttgcttcaa tcaaatcaat
    19621 gtcatcaatt gtcagctgag cctttgcaag agcctttttc gtagcaggta ttggtccgca
    19681 gcccataata cttgggtcta cacctgcact tgcataacta gtgattttag ctaaaatagg
    19741 gagccctaat tcttcagctt tttctttact catcaataaa attgctgctg ctccatcatt
    19801 gattcctgaa gcattaccag cagttactgt cccctcatct ttgataaaag cagggcgtaa
    19861 ctttgctaac ttatccactg tcgttccaaa tttagggtat tcatcttgat caacgagtaa
    19921 aggttcacct ttacgctgag gaatagtgac aggtgcaatc tcttctttga aacgaccttt
    19981 ttctatggca agttgggctt tacgttggct atcagctgca aaggcatctt gttcttctct
    20041 ggtcagtcca tactgttgca caatattttc ggctgttata cccatgtgat aacctgcaaa
    20101 agcatcactc aacccatctt taagcatggt atctactact ttactatctc ccatacgaga
    20161 tccccaacgt tggccttgta aaacgtaagg tgcttgactc atgttttcgg taccaccgac
    20221 taaaacaata tcagcgttgc ccgtttgaat cttttgagca cctaactcaa ctgcttttaa
    20281 gccagaacca cagaccatat caatcgtaaa agcaggggta tcataagaaa gccccgcatt
    20341 tagcgctact tgccgagcaa cattttgacc cagacctgca tgtaacacat tcccaaagat
    20401 cacttcatca atggcatcac ttttaagacc ggtatcagct aaaatttttg taacaactgt
    20461 cacccccaaa tctacggctg ataaagattt aaagactcct ccaaaatttc ctatcggggt
    20521 acgataagca gatgtgatca ccacttcttt agtcatagaa acttctcctt tttcattatt
    20581 gaaacgctta catttttatt atataatgtg aactataata aacaaatatc gtttttttat
    20641 atatgctata actaaacgtt atcgaaagag gaaaatatga atatttcaag cttggcttat
    20701 tttattgaga ttgttaattg tgactttaac ttaaccaaag cagcccaaaa aagtcatatt
    20761 tcgcaacccg cccttagtag ttatatcaag actatcgaaa aagaagaagg agtcgtttta
    20821 ttccagcgtc aaaaagggcg tctagttgga ctaacctcag tcggcgaacg cttttttgaa
    20881 aatgctaagg tggttatcgc atcacatgag caattaatgg ctgatttgcg gagtgaatcc
    20941 aaaaaataca aaggagaaat ttcaatcggt attcctcaac tcatctctgg cattgttttt
    21001 tctgagctca ttccaaaact catcgaagaa aatcccgaag ttaaatttca tattgtagaa
    21061 acgggtgctt atgaactgca aaaaaactgt ttctaaaaga agtcgatttt gcgattttat
    21121 taacacccac tacactagat gctactattg ctgatatcga cattctccta agagatcaac
    21181 tctgccttta tatgagtcac aaaacctttc aagagacgtt tgatagtaat cctaacatct
    21241 ctatcaaagc attacatcgt caaaacttag taacttttga taaaaccttt atgattaatc
    21301 accagcttac caccttattt gaagaagcaa atagtcttcc tgtagtcaaa tgttattctg
    21361 cctcgtggga ttttttatta aattgcacgc gctattcctc ttacctaaca atcctaccaa
    21421 ggcctattac tcattttgct catatggacg gtttagtgga ggtccaactg accgaacatc
    21481 caaaatggga agttgttcta gcctctctca agcacaataa aacaagccat ttaaaacatt
    21541 atatcaagca caccatctta gattatttca tgaaccagcg cttctaattg cttgactcag
    21601 accttaaaaa aaaacgcctg aaggaaacaa tcccaagcgt ttcatatagt tatttcctaa
    21661 aatacaaata ggtaaatgat ggcaatacct gtgaccacca agacacaagg aatagtataa
    21721 gccacaattt gattgaattt tagtttgaag tttgagaggt aagataaggc ccaaaaagga
    21781 actatcaagt ttgttgtggc atctcctaat tgatacgcct gcaaaatctt acctaggtca
    21841 ttaccaactt cgatggttgc agggacgata tagggagctt caatcacaaa ttttgagcct
    21901 cctgatggta ccgcaatatt cagcaaagcc gagtaaagat aagcaaagac cggccatgtt
    21961 gtcccatttg aaatagcgac aaagaaatgg gaaatctcta gtcctagacc tgtactttga
    22021 atgattccaa aaataccagc gtaaaatgga aactgaagca ccaagcccca agatgacatg
    22081 accccgtctt tgaataagga accataatat tctggtcctt gttgaccaca aagaagcaac
    22141 cctaagccta aaaataaaaa gttaaaacta ttgagatcta gaccagacaa gcccaccacc
    22201 gaaaaatgat aaaggctata gctaacaatg gccccaccaa caagatacat cactaagcga
    22261 gatgcgttca tcttctcagc aggtgtgttt cttgcaataa caacatgtga agcggtatca
    22321 agactatggt gatagatctc atcatcaagt tccatgattt tagtttcatc ttttgggtgc
    22381 gccaataaag caaagcaaag cggtaccagc atacacacca ctaaaagact gataaatgga
    22441 agattcaaaa ctgattctgt cagaggaaca ctttcaggaa caacctgttt ataagcatct
    22501 gcgacactat ttcgcaaata atctggagta gccgaataaa gcaccgcagc accagataaa
    22561 ccagaagtag ctggtaaaaa ggtaaaaaat aaagtagcaa caaacaaagg tgtatgcacc
    22621 ttaatccttt tttgcctagc ctgaacaagc aactctttcc caaacacaat ggcaaccatc
    22681 ataccaagac cccagtgtaa aaagccaaag atagaaccta ctacaataga aaaaataaaa
    22741 atctgagtcc tggtttgagg cagtttggca agggacttaa acattttatg agcaggtgga
    22801 gaactcgcta cagcattccc tgtcgcaagt aaaatagtca tctgcatggc aaaggacaac
    22861 agcccccaaa aaccatttac ccagccatag ataatcccgc ccgtcttttc cgtaccaata
    22921 aagggactat ccgtcatcaa cacagacata gtaacgacta aaaaggttaa aataaaacag
    22981 ataaaaagcg attctggcat ccatttcata aagccatcaa tatagcgatc cataaacctt
    23041 tttttggttt tgatgttatt catgttgtca attcctttca cattgtccac aagcactttt
    23101 gttcttttga tcagtaacct atcgataata ttaataagcg tttctttagt tcatttagtt
    23161 atcaataagt ttaaacccac aaaaaacact tttgttccct ttttattatg acaacgtttt
    23221 cataaacaaa caactatgat ttttttattg cttccataac ccttatttat agcaaaaaga
    23281 atggcgcttc actcatcaat aatgtctttg ttatatttaa ataattaaat aattatgata
    23341 aaaaattaaa aaataattta ctttttagtc tttttgatat aatatattag tattaaaaga
    23401 aaggtttttc atgatatgaa acgatgtaat aaatatctct tcacttcact actagcagct
    23461 tcgatgctgt ttagttcgta taaatcagta catgcccatg ataacattga tgagaaaggt
    23521 aaagtgcacc tttactggca gggaaattac tatgttgata actatgtcga ctatactaaa
    23581 acattggtcg ataacgataa aaatatcgaa tggactgtaa cctttaattc agctaaagaa
    23641 caatgggttt accctgattt ctcagttttt cttcctaagg gtgtaaaagc tcctacggag
    23701 ataacttatg agcatcatta ttgggacggt acagttcatt ctgaaaaacg caggaataca
    23761 caatggcatt ataattggga aaaacaaaaa acaaatttta atcaagaatt tgataaattc
    23821 cctggttata ctggttggag tccttctcta gataaatttt acaaactaaa aaacgacgga
    23881 aaattttctc acgttttagt agacacctat ggccgtcaat cacacactta tttttctcat
    23941 aaaatggttt ggaagtttaa aactgagctt gaagaagatt ataaaaataa atggaataaa
    24001 ttaccgttta tagcaggtat taaacaaaac aacccattag cagcatcatt cccaagttat
    24061 aaaggggagt tcggtgagta aatgaagctg ccagtccatc atcttttgca taattacagt
    24121 cagagtctga gacaacatga ttgctcagac tctttttatt atgcttgaag tggccgaaat
    24181 actttcgtaa gtgcccaaca agattaaaaa ggcagaaaac cttttaaggc tctgcctttt
    24241 tgaactactt aaaatgactt atctagtgaa ccgagacgat cgattccaga aaaagaggcc
    24301 cactaataag tggacccttt ttcgctattg tcaccaacag cttatcttaa catgatgata
    24361 ggcgtttttt ggattttagt gatattacaa aaacactact aataagtgag caagaagtaa
    24421 ccatccaaaa gaacttgtta tgttgcttct caccagttgc tggtaaacta gtattactct
    24481 ttggcttatc ttttttagga atctcagagt tactttctgg ttccttgtta tcaaagtgga
    24541 aggattgtac tagtttagta tcttcgactt ccgttttaca accagaatcc ccgtgcatgc
    24601 cagtttgggt atcctctgtt gtctcgacaa cctgcccctg accaccaatg ataacgtctg
    24661 aagacttgct atcttctatt tcagtagttg agccagaatg gcctgtcata ccacgttgtg
    24721 tgtcttctgt tgtgtcaaca acttcacctt gaccgccaat gataaggtct gaagatttac
    24781 tgtcttctat ttcagtagtt gaaccagaat gaccttgctc gtctggaagc ttttcttcaa
    24841 tatcaataac ctgacctgaa cccttagttg gcttgtaagc atcaaccatg acaatatgag
    24901 cgtcaccttt agttgctttg ccatttacag taacctgacc ttgctcatta actgtaaagg
    24961 taatagcagt tgctatctca taaccgtctg gtgctgcggt ttcgacaaat gtatattttc
    25021 ctggcatcag gtagaaatct ttcacttgtc catctgaaat ccatgtacta atagttttac
    25081 cagatgaatc acgcaactcc atagttgcac cagctaactc tttgccgtca atatcacgtt
    25141 ttgagaattt aatatgggta gcactatctt cttcaattgt catatcaccg gactgacctt
    25201 gctcacttga taaacctgac aaggtatcaa ctgggcgtga gtcttcgaca gtagtggtac
    25261 cacctgaagc acctgattct agcggaagtg tttcttcaat ctctaggttc ggtccttgtt
    25321 gtgtaataac ctcacgtttg gtattaataa tatcattagc tgagtaagtc acatcataac
    25381 ctgttggaac agttacttct ttaacagagt aaatgatgtc attacctgtt ccattatact
    25441 tcttcaagtt tttaaactca aacgaccact cattacctga gccagaaata aatttggttt
    25501 gaccttccac agccactcca tcagcataaa gctgcacttg aatggcttgg ggacgatttt
    25561 ctgccgtatc atttttccaa attttcttac ctttgaaaga cgttagcgtg tcttcatcaa
    25621 tgatttttga cccagattga ccttttattt cagcatttcc aaattcaagg ttaaagttat
    25681 tctgttcaaa cacaaattct gtttctctgg tgttaaatat atcatttcct aaatacgaca
    25741 ccttgtagcc gtctggaact tttacttctt caactgagta cttatactcc tgattcttgt
    25801 catcgtactt aggcaagtct ttgaagtgat aagaccaatc gttatccttc gttacttctt
    25861 gaactttatc ggacattttt tgaccatttt gcaacagttg cacttgaatc tttactgggc
    25921 gttgatctgg tctgtcattc tcccaaattt tcttccctga aaacgtaagg gtttctttat
    25981 ggttgacaat cgtgacttct ttgttaactt ctacttttac aaacggatgt gtctttttcc
    26041 acttgactat ttcctcagca ggtttggtag tatcaacagt aacagtcgcc aatttctctg
    26101 tcacctgctg atagccttta ggtgcttgtt tttcatacaa gtcgtaggtg cccgaggtca
    26161 ggtccttaaa gtgaacctct ccttgttcat ttgattttgc ttctactttt tgactgtcac
    26221 tagttgatga gcgcagctca aaggccgctt ctggcaatgg cacattgtca gttgacattt
    26281 tcttaaagcg aagctgagcc actcgtttta gatttgctac tgtaatagag ggcttagtaa
    26341 attcacttgt cccagaacca ctacccttag aaggagacaa ggcaatttcg ttttggaaat
    26401 agacattatt agttggattg ctagatttta cagcaaattg gtcattaaaa cttgccaaat
    26461 tggattgaac gactagtggc ttaccagatt ggtctgtttt ccctgttact ttaacaacaa
    26521 acctttttcc atctttaagg ttatctttaa aatcaatccg ctgacgtttg gtcatttgaa
    26581 aaccatttcc taggcctgct gtgatatccg ttcttaaagt aagttttgta acatcaaccc
    26641 cataacttga tggtaaatgg taatcagaag gcacttcata aatttccgca tttaataact
    26701 gagcactact gctattagaa tcagttgaga aagcttgttt atctgttaac cttccaaatc
    26761 cccataaatt catggtcgca taaggaatat tggtacgatt tgggttgaca taaacatacc
    26821 aagtaaagtc gcctgtttgt gtattgtagc tttcaatact tccacccaca ttgctcaatc
    26881 cgttagcaac atagttgtct ttttgatttt tttcatttcc gtaaataaca ttaactgtcc
    26941 caccgtattt gatctcctga ccaccaatag tactcttaaa atttgaaatg ttggtgtttt
    27001 ccaacacttt cttattctct aggaataagc tcaattctgc agacaactgg actttatcta
    27061 aacctgcaat ataatctgtg aaagtataga taagttgatg gttctcggca tggtattttc
    27121 caatcgcaag cggactattt gcactgtcgt aaatgatttt agggatatct tgactgatac
    27181 ctttaggatt gagacgttgg tccagctgta acacaaaggt atcgcctggg ttgattgttt
    27241 cagatttatc cttttgtttc aactcaaaag acattttaaa ataggcggca tggttgcggt
    27301 agaaatccgc tgagtttgac gttttttctt gttcaccgta cttggataca acagacattg
    27361 ttgatttttc taactgtaaa gactggctaa catcttttga ccctgctcta ctgatgattt
    27421 ccccattata gggattttca accaacttgg tatagccgtt ctcataaaca gtcactgtcc
    27481 aagtccgact ggttttatca taaccatctg gtgctttatc ttcttttaag gtataagtcc
    27541 ctggtttaat atccttagca tcaatcttgc catctgaacc tgattcaaag gtctgaacag
    27601 atgctccctt cccatccttt ggtgtcagtg aaaaagttgc tcccgataag ggcttattgt
    27661 tttggtcgac tttcttgatt tcaaaagctc cttgtttgtt tgctccattt cttgtttccg
    27721 catgtcctac tgatactctg gacaatccta taaaaaccaa taataaacct aaaataaatc
    27781 ctgttaagga taacaggaag cttaacttat agcttttttt tgtgtcattt attttctctc
    27841 tccacattca taagcgacag cctatgtcca aaactaaaga ctacaggaca actgtcaaat
    27901 agttatttta ccattttcag ttgtcaagtc tacatcttat ggttagaata tttattttga
    27961 tggtttttta gcgtttttta tgtcattatc tgtaactctt tctttttcaa gcgcttaacg
    28021 tacaagtcct ttatctttat ctgcttagct attcatagta taattaaggc aggagatgat
    28081 ggagagagag attactatta ggaagacctt ttagtgtgcc taccaaatag gaattttcat
    28141 cattaacatt tactaaagct tttacaagtc taaggaaaat gatacaacta aggatggggg
    28201 caatctatca aatggttata ttcgatttaa aacatgtgca aacattacac agcttgtctc
    28261 aattatctat ttcagtgatg tcacaagata aggcacttat tcaagtatat ggtaatgacg
    28321 actatttatt atgttactat caatttttaa agcatctagc tattcctcaa gctgcacaag
    28381 atgttatttt ttatgagggt ttatttgaag agtcctttat gatttttcct ctttgtcact
    28441 acattattgc cattggacct ttctaccctt attcacttaa taaagactat caggaacaat
    28501 tagctaataa ttttttaaaa cattcttctc atcgtagcaa agaagagctc ttgtcctata
    28561 tggcacttgt cccacatttt ccaattaata atgtgcggaa ccttttgata gctattgacg
    28621 ctttttttga cacacaattt gagacgactt gccaacaaac aattcatcaa ttgttgcagc
    28681 attcaaaaca gatgactgct gatcctgata tcattcatcg ccttaagcat attagcaaag
    28741 catctagcca actaccgcct gttttagagc acctaaatca tattatggat ctggtaaagc
    28801 taggcaatcc acaattgctc aagcaagaaa tcaatcgcat ccccttatca agtatcacct
    28861 catcttctat ttctgctcta agggcggaaa agaacctcac tgttatctat ttaactaggt
    28921 tactggaatt cagttttgta gaaaatactg acgtagcaaa gcattatagc cttgtcaaat
    28981 actacatggc cttaaatgaa gaagcgagtg acttgctcaa agttttgaga attcgctgtg
    29041 cagctatcat ccatttttcc gaatcattaa ccaataaaag tatttctgat aaacgtcaaa
    29101 tgtacaatag tgtgcttcat tatgtcgata gtcacctgta ttccaaatta aaggtatctg
    29161 atatcgctaa gcgcctatat gtttccgaat ctcacttacg ttcagtcttt aaaaaatact
    29221 caaatgtttc cttacaacat tatattctaa gtacaaaaat caaagaagct caactactct
    29281 taaaacgagg aattcctgtt ggagaagtgg ctaaaagctt atatttttat gacactactc
    29341 attttcataa aatctttaaa aaatacacgg gtatttcttc aaaagactat cttgctaaat
    29401 accgagataa tatttaagaa ttcaagacac tcttttcatc aatcattatc ttatatggta
    29461 caaaaaccta tcagcataga aaaacatctc caaagtcaag caagactatg gagatgtcaa
    29521 taagtttcat tataaaaata gagcaactag aaatgggttt tatagcctac tcttcagttt
    29581 tttaacataa agaagaacta gtaaccccag taatacgata cttaagatac ccaataaact
    29641 ttttacttca ccagctaatg gtaatggggt tttagggatg tcaggttgtc taggcggtat
    29701 tggttttact aaccgtttgg gcttaaaagt aatcgctgat ttttcttcgt ctccagccct
    29761 tcgagaaata acttttgcga ctagagtccc atcttcatca taggtcacat agacaagaac
    29821 gtcaaataca gtagtatctg cttgataatc tttattttgt gaaggcttct gataaacacg
    29881 ataagtatat tgcccaactg ttgtgaaggt cagaggggaa aagctcgctt ttccagaacc
    29941 agcaattgtt atttcttcta ttgttttcat cgcatcaatt gattctaatg caatcgaaaa
    30001 tggggtacta tcgcctgctc tctctaagac attttcgaca ctaatgctag tttgaacagt
    30061 gctgtctttt gctaaaacag tctgattaaa ggccagcatg gttaatatca ttacgacaga
    30121 aaataacatt ttccaatatt ttctcaaaag tctcctcctt tcgttattct tgaatagtac
    30181 cgacaacgat aacacgattg tcagtagaaa aattttcaca cgttgaaaaa gcaacgaact
    30241 ttgtatgatg cttcaattta acaggtttaa attgttttga tcttttactg atataatcaa
    30301 caagctgcct ttgttggtct tgattggtaa tagcattagg attaaaaact aactggtcaa
    30361 aggcatctgt cttgagacaa gcaaaaatag tgacggttag tttttttctc tcttttgttt
    30421 caatgatagc tttattatgt ttgttgaaaa aatccttttt taaaaatttt ggaatttcgc
    30481 caaacatggc attacctgcc atatgatggc cataaatcag agagtaatcg tccgtaaaat
    30541 cattatgatt ccgtgtatct aaaaataaac taccagacat ggcaacactg ccatcaactg
    30601 ctttattaat atactctaaa ttcgtttttc cctgaactag aggataatca atatgagtcc
    30661 ctgggatatt taaccaacca atgacatcct cattcaaagc taacaagtct tcaaatttag
    30721 gctgttgttg agctgtttta aattttttga aattagaagc gtctgcttgt tgatagagat
    30781 gataagaatc ccacaaacca aaaccagcta aaaatagtac gactaaacaa aagataagaa
    30841 tgagagtatc aatggcttta ttgataacct gtacaattgt catcatcatt ctttgactaa
    30901 tcacttaagc ttttttacgt tttgtaatat agataactcc accaatagcc acaatgctaa
    30961 gaactgcaaa tggagcaagg gtccctacaa caccagttgg aacttgagtg tcacgcttat
    31021 ttgtgacaac gatttcgtca gcagattcgt ctgttttctg gttttgagta ctcaaagtga
    31081 aatcggaact cttttctttg ccttgttctt ttaatgttgc cgttgtagtg taaccgtctt
    31141 tattcgcttc gtcttcacta agatagtaat tgataccaat tggtaatttc gataacatga
    31201 cactcttacc gtgtcccaaa gtaaatttgt aaggcgttcc tatcgtcaca tcttttttac
    31261 ttccatcctg ttcgattttc acaactgatc cggttttata atattcactt ggttgtaatg
    31321 ttaatgtgaa gttgaatgat tttttacgat ctcctgcatt gccagttatt tgtttttcaa
    31381 tttttaagga agtagtttta atagagtttg taaattcaat tggtttttta gtactagaag
    31441 taccttcttt tgatacaata tatagaactt cgaaaccttc ttcgttattg gccttattcc
    31501 caacataaac atctacagtc cactttttat catcgtacct aattccgtct tttttatcgt
    31561 ttttctctga aaccatatag cggtaaacac ctatagctgg aaatttaacc ttagaaaaat
    31621 caaattgtgc tattttagtt ttttgagatg ttttatctgt attcttatat tttactgtta
    31681 cttgtttgtc taagccttct acaattccat ttttaatatc taggtcgcct tcttttccac
    31741 ttgcagtcat atcaggctct atagtaaaag taaaggcggt ttcgggcatt aacacctctt
    31801 catcatcata agtcattgtc tttgtaactt gtagtgattt tcctgttaca actcctgccg
    31861 tctcagcttt tacgttttga tttaaagaag ctgttcctaa agcagttgct aagattgcag
    31921 tagcaagtaa taatttattt tttttcattt tctctctctc caaatttttt taaattcctc
    31981 tcactcttaa tagagttgag attttccctt tgatttgatt gatgggtaac gccccataat
    32041 aacgactgtc caaacgttct tcacgataat cattcaatat gaaatacgta cccgtaggaa
    32101 ctttataggg aaaatttggg ccacttgagt gaggatacgt caggtaaggg acctcttttt
    32161 ctggtggatg cccattaatc aacagtcctc cttcttgcgt aaaattaacc tcatcgccag
    32221 cttgagcggc aattcgacca actttcaaag tgttatcaac ctcatagacc accacatcat
    32281 taatatgata gcgatcagtc aaacgataat ataagactcc atcaccagca cttacagcag
    32341 gactcatctg atttgtgtta acaatcatga cgccaaaaac atactgaaat agaatgtaac
    32401 ctagaaatcc aataatagaa atcttaagta ctaaccgtat gaaagcttta aatagtggat
    32461 tctcatttaa gcgatttagg taattagtca ttttttagtc ctttacgacc aaagaaccac
    32521 actaacaaac caaatgggac aagcaataat aaccacagat aggttccacc atcagtagta
    32581 aaaccagttg gtgggacaag atctttacgg ttttcaaaag tgacttcttt gtctgctgtg
    32641 acattctcac ttgctgattg agcttcttga ctaactttgt tatcaacggt tactatataa
    32701 tccttagctt cagtctcttt cagagtataa gaatatcccg tcggtaatcc ttctattctt
    32761 atggtgtcac catgctttag attaaatgaa tatttcccat ctttagctac taaatcttga
    32821 ttattagttt ttagagtgtt aacaataggc tgtccagttt tatctttcaa ctcaagttca
    32881 aattggaagc ctttagtttt atctcccaac tcaccgacta ctgttttttt cactgtcaaa
    32941 ctgtgagtta ctggaataac ttcttgcttt ttatcttcca tacgaatcac gtcaaccaaa
    33001 tcatctggat ggtattctgt cccaataaga gattggtatt tgctattatt aggtacgaag
    33061 aaatcaagat ttgttagttg aggggcacta ttatcttgag cgtaattaat taattctttt
    33121 gttacagcta gggttttttc atccatagat tcaaatccat ggtaaccttt cccattgtta
    33181 taagttttta aggtttttaa gtcagtgctg tctgtaaagt aatagatagc aagctgagta
    33241 gccgcgcgaa actgtgtttc tgttaatcca ttatagctat cacctttttt attgtagcct
    33301 ttttcaatta cttttttaat gtgctttaag aagtcttctg gatttgtatc tctcggtctt
    33361 agcgcgtatt taaacaaatc actacctgcc gtatgtgtgt acttgacttc tttcatcgta
    33421 ctaatatcag gatctatagt tcctcctcca tcctctgatt caggtggaga gtgtaaatca
    33481 gcattaaaac agtagacaac ttgtgaactt ttatccttat ttttagcgta ataaaatttc
    33541 ccataaggag tgaacgtttc tggattaata tagttactat cttgcatatc gctgtacgct
    33601 tccactgagt atggctctgc tacttctttg tttggatttt ccacttgaga accatctttt
    33661 tggacgataa atactttttt attcactact ctaaacttaa tcggctccgc aattttatat
    33721 ccatctggag atgatgtttc tgttaaggta taagtcccat ttgataattc aatcttttct
    33781 cctgttccat tactttggaa gactttttct tgaaaatcta ggatatcttc ccctgttaaa
    33841 cgcaaagttg ctccctctag aagtttagag tagtcacctt ccgcatattt tctgataatg
    33901 actgatgttt tttttgtttg cactggcggt tctggagact gacctggtgc cggaggggat
    33961 tcaggcacgt actctgcact taataaattt tgaacagatt tatcttgcgg aacaaaaata
    34021 ttcagtttag atccttgagg tagcttattt ttagatgttt cttctaaatc atcactaatt
    34081 agttttgagt aagcttcacg cattaatttt acttgttcaa aatctatttt tccgtcttta
    34141 agctcagatg tccataacgt ttttatatta ccataagaac tgtcagaata gtaccaaaca
    34201 gcttgttgag ttactaaaat agcatttaat ggttctatct tatccatata gccattagca
    34261 tttttaggat aagcgttgta cataatactt aaaagtttat tattcaatga ttctccacga
    34321 atcttaggtg tctgagcata atttgtgaac acatcacctg tcccatcaac tctcttaaac
    34381 cattgtttag aaaaagcatt ttttctattc ggataatgag aattgatatt aaagcaatat
    34441 acttggtagc tcttacttcc atttaaatta acccttaaat catgtgctac tttaaaacgt
    34501 tcatagtaag gatgactaga gtcatatgaa tcatagccat accaagggta ggcatcatct
    34561 ggacttttta ttttaggaac actgacttct tcaattctct tttcctctcc aaatatgctt
    34621 tgagcacctc tcatggaact taccaaggtc aaaaacatta aaaatactcc cactaaagtg
    34681 acagttaatt gttttggctt tctgtttgat acccgaggag catcttgctt cttttgcaat
    34741 tttttgttat tcattccctc tccttctgaa taaaatcttc agtcccatcc ctttaccgga
    34801 cagtcacaat gctatttcat agctactata tcatttattt gatgtttaaa acagttgttc
    34861 ttatcatctt tttttccata ttatgcgttc caaaaaataa tttttgggaa aatcatttgt
    34921 aaccgcctaa tatttttctc ttgatgagat ttaattaaac ttcatcaaag gggatagtaa
    34981 ctaccagatt attttacttt tacaacagtt tcagtacgat aatgaagatt cagggcaagt
    35041 tagtttttaa tcactttttt atcagtcatc tttgaataca taaatactaa aataatagct
    35101 ataaccaccc cgaaagcagc acatactact tctgtccaca ttcttgattt atccacgagt
    35161 ttcattgctt ttttagcctg ttgatacttt ttgtaatgaa atgcaattct atgacctctg
    35221 accaatagac gcttggtatt aacaccatat ggtgtgcagg taaccagagt agcataatct
    35281 tttcccatta cctcagaaag agatgtgact tgatctggtt caacagttag tatctgatca
    35341 actttataag ccaatacttt atttaacaca cgaaagtaaa aagtatctcc ccttttcact
    35401 aaattcaaat tagtaaacat ttctgccgat ggaaggcctc tatgagcaga aataaccgtg
    35461 tgagtcccgt cgccaccaac tggtaaggca cttccaaata agtgacctgc tcctttagtt
    35521 agcacctcgt ctgtcgtata gtggtatatg ggtaacgtca ctttaatgga ggacacttca
    35581 acatattcca taatatcatt gttttcaatt tgaagtaggc tttcgtagtt tttatcatga
    35641 atgccatccc taaatgaaaa agcatctgga acgggctgaa tcccaagcct agcgttatac
    35701 gccttagcct tttgccacat ctcgtccatt tctgagggct tcttttgagt cacttgtttg
    35761 tgataagttg ataacaatta acgatcacga tagacattcc actgactact tagcgtaggg
    35821 taaagaaaag tggctgttcc caaaaaaaat aataagagta gccctagatt ttttagtctt
    35881 tctgtcattg cagttcctta tcaataaaaa tcgagactgc ctaaagacag tctcgattga
    35941 tttcgacttt tgaaagcgtt aagctttacg acgtttaaca ataacttgat ttcttgtaag
    36001 tctattatac atagaataca ccatttttta gccatcctac ttgctctgaa ttatctgtca
    36061 ccttattatt atcagatgta taagtagtac cttttgaatt aacatttgtc ttgactgtag
    36121 cagttatcca ctattcagca tatttgcgca ttatttctta tggaacactt ttacgtcaga
    36181 ctttattgtt ttgtttgttt tttaaaaggc taaaaataga aagtactgac aaaataagga
    36241 ttccaagaaa ggctaaaaca tttccaatat ctcctgttgc aggaaggatg ggtgttatat
    36301 tttttgtagg tttttcccga ttctcttcca ctttgggctc attattgtca aaatggaaca
    36361 ctaacttcgg acgcgtatct tcaataatag tcactgtttc actgaaacca ctcatgcctg
    36421 tttgagtgtc tttagtaaat tcaacagact cactttggcc tcccatcaac actcctggct
    36481 ctttcgtatc ttctgtctca acctgaggag ttgtttgacc actcatgcct gtttgagtgt
    36541 ctttagtaaa ttcaacagac tcactttggc ctcccatcaa cacttctggc tctttcgtat
    36601 cttctgtctc aaccatattt cctgaaaaac ctgtttcatt cggaagtttt ttatcaatat
    36661 caacagggtt ctgttgattt ccatagactt cggtaatata ctccgtctca ccagactcac
    36721 ccgctagagg tgactggtct ttattgttaa actcataacg aggaacttca ataggtaagt
    36781 caacactagg tttttcaggg acttcttgac catctaactc tggcattaat gggggaagcg
    36841 caggttctaa g
//
LOCUS       DAUCLR010000008        36278 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.008, whole genome shotgun sequence.
ACCESSION   DAUCLR010000008 DAUCLR010000000
VERSION     DAUCLR010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36278
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.008"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..1296)
                     /locus_tag="VGT87_000298"
     CDS             complement(<1..1296)
                     /locus_tag="VGT87_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEQ3813188.1"
                     /translation="MIISRSRGKTMKIDKRHLLNYSILLPYLILSVIGLIMVYSTTSV
                     SLIQAHANPFKSVINQGVFWIISLVAITFIYKLKLNFLTNTRVLTVVMLGEAFLLIIA
                     RFFTTAIKGAHGWIVIGPVSFQPAEYLKIIMVWYLALTFAKIQKNISLYDYQALTRRK
                     WWPTQWNDLRDWRVYSLLMVLLVAAQPDLGNASIIVLTAIIMFSISGIGYRWFSAILV
                     MITGLSTVFLGTIAVIGVERVAKIPVFGYVAKRFSAFFNPFHDLTDSGHQLANSYYAM
                     SNGGWFGQGLGNSIEKRGYLPEAQTDFVFSVVIEELGLIGAGFILALVFFLILRIMNV
                     GIKAKNPFNAMMALGVGGMMLMQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVA
                     VGFVLNIDASEKRDDIFKEAELSYRKDTRKENSKVVNIKQ"
     gene            complement(1436..4234)
                     /gene="ppc"
                     /locus_tag="VGT87_000299"
     CDS             complement(1436..4234)
                     /gene="ppc"
                     /locus_tag="VGT87_000299"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEQ3813189.1"
                     /translation="MIEETSKEGDGHLPLKKLESSNNQTIIAEEVALLKEMLENITRR
                     MIGDDAFTVIESIMVLSEKQDYIELEKVVANISNQEMEVISRYFSILPLLINISEDVD
                     LAYEINHQNNTDTDYLGKLALTIKDLAGKDNGKDILEQVNVVPVLTAHPTQVQRKTIL
                     ELTTHIHKLLRKYRDAKAGVINLEKWRQELYRYIEMIMQTDIIREKKLQVKNEIKNVM
                     QYYDGSLIQAVTKLTTEYKNLAQKHGLELDNPKPITMGMWIGGDRDGNPFVTAETLCL
                     SATVQSEVILNYYIDKLAALYRTFSLSSTLVQPNSEVERLASLSQDQSIYRGNEPYRR
                     AFHYIQSRLKQTQIQLTNQPAARMNSSVGLNTSAWPSPASLEKPILAYDSPVDFKADL
                     KAIEQSLLDNGNSALIEGDLREVMQAVDIFGFFLASIDMRQDSSVQEACVAELLKGAN
                     IVDDYSSLSETEKCDVLLQQLMEEPRTLSSAAVAKSDLLEKELAIYTTARELKDKLGE
                     EVIKQHIISHTESVSDMFELAIMLKEVGLVDQQRARVQIVPLFETIEDLDNARDIMAA
                     YLSHDIVKSWIATNRNYQEIMLGYSDSNKDGGYLASGWTLYKAQNELTAIGEEHGVKI
                     TFFHGRGGTVGRGGGPSYDAITSQPFGSIKDRIRLTEQGEIIENKYGNKDVAYYHLEM
                     LISASINRMVTQMITDPNEIDSFREIMDSIVADSNTIYRKLVFDNPHFYDYFFEASPI
                     KEVSSLNIGSRPAARKTITEITGLRAIPWVFSWSQNRIMFPGWYGVGSAFKRYIDRAQ
                     SNLERLQHMYQTWPFFHSLLSNVDMVLSKSNMNIAFQYAQLAESQDVRDVFYEILDEW
                     QLTKNVILAIQDHDDLLEDNPSLKHSLKSRLPYFNVLNYIQIELIKRWRNNQLDENDE
                     KLIHTTINGIATGLRNSG"
     gene            4393..6192
                     /gene="pepF"
                     /locus_tag="VGT87_000300"
     CDS             4393..6192
                     /gene="pepF"
                     /locus_tag="VGT87_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEQ3813190.1"
                     /translation="MELKKRSEFPENELWDLTALYKDRQDFLLAIEEALQDIDLFKRN
                     YEDRLTSVDDFTQALIEIEHIYTQMSHIGTYAFMPQTTDFSDESFAQIAQAGDDFMTK
                     ASVALSFFDTALANADLDVLDTLEKNPYFSAAIRMAKIQKEHLLSPDVEKALANLREV
                     INAPYDIYTKMRAGDFDMDDFEVDGKTYKNSFVSYENFYQNHENAEIRDKAFRSFSKG
                     LRKHQNTAAAAYLAKVKSEKLLADMKGYTSVFDYLLAEQEVDRSLFDRQIDLIMTEFG
                     PVAQKFLKHVAQVNGLEKMTFADWKLDIDNDLNPEVSIDGAYDLVMKSLAPLGQEYTK
                     EIERYQTERWVDFAANANKDSGGYAADPYKVHPYVLMSWTGRMSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNTHMSTYYVEAPSTFNELMLSDYLEHQFDDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGGTFGADQLNAMMKEVLTDFWGDAVDIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVMSTAGYLNLKNNPDGAKEWLDFLKSGGSRTPLDTAMLIG
                     ADIATEKPLRDTIQFLSDTVDQIINYTEEMSHA"
     gene            6185..6664
                     /locus_tag="VGT87_000301"
     CDS             6185..6664
                     /locus_tag="VGT87_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEQ3813191.1"
                     /translation="MPNLYDFTVKAQDGSDISLVNYKEKVVLVVNTATKCGLTPQYQA
                     LQALYDTYHDKGFEVLDFPCNQFLNQAPGDAEEINHFCSLTYHTTFPRFAKIKVNGKD
                     ADPLFTWLKEEKSGPLGKRIEWNFAKFLIDQNGQVIKRYSSKTDPKLIEEDLKALLG"
     gene            complement(6710..7093)
                     /locus_tag="VGT87_000302"
     CDS             complement(6710..7093)
                     /locus_tag="VGT87_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEQ3813192.1"
                     /translation="MNKGFKIGFLALVAMLLVACSQGTKQTQTPSVPKSDHHVRLVVK
                     EDTNTVDEKVSFEEGDTVLEILKDNYEVKEKDGFITAIDGIEQDMKANKYWLFKVNGK
                     MADKGANQITVKDGDSIEFYQEVFN"
     gene            complement(7077..7580)
                     /locus_tag="VGT87_000303"
     CDS             complement(7077..7580)
                     /locus_tag="VGT87_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEQ3813193.1"
                     /translation="MSAKLMSRIAIMSALCVVLRMVFSSLPNVQPVTAFLLSYLLYFG
                     LAEAVLVMMLCLFLSAFLLGFGPWVFWQVTCFVLVLLLWRFVLYPLSQQFPKYQLGCQ
                     AFLVALCGLLYGVLIDTCFAYLYSMPWWSYVLAGMPFNIAHALSTLVFFPVIMMLFRR
                     LIGEQRI"
     gene            7905..8753
                     /locus_tag="VGT87_000304"
     CDS             7905..8753
                     /locus_tag="VGT87_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEQ3813194.1"
                     /translation="MRRKIKPIVVLVFFILLAMVLIIGKRQANHAKQKEVEDAKSHIP
                     IATSNPSKAKASTSETEDFILNPIVDVSGWQLPEEIDYDTLSRHISGAIVRVYGGSQI
                     TAHNNAAFTTGIDKSFKTHIKEFQKRNVPVAVYSYALGRSTKEMKEEARAFYKNAAPY
                     NPTYYWIDVEEATMKDMNKGVTAFREELKKLGAENVGLYIGTYFMAEQDISTKGFDAV
                     WIPTYGSDSGYYEAAPNTTLDYDLHQYTSQGYLSGFNNALDLNQIAVTKDTKKTFEKL
                     FGNSNN"
     regulatory      8880..8976
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9021..9584
                     /gene="thiT"
                     /locus_tag="VGT87_000305"
     CDS             9021..9584
                     /gene="thiT"
                     /locus_tag="VGT87_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEQ3813195.1"
                     /translation="MSPNTNVKYLIEAAIFAALAMALSFIPDFAGWFSPSYGAIALVI
                     FSLRRGLKYGMLAGLIWGLLHFVLGKVYYLSMSQVFIEYILAFTSMGLAGSFSDSLIK
                     TLRRQQTFFAVFLAIMASLLAVTVRYLWHFLAGIIFWGSYAPKGMSAVWYSFSVNGTA
                     GVLTFLITCLALMIALPIHPQLFDPKD"
     gene            9675..10154
                     /locus_tag="VGT87_000306"
     CDS             9675..10154
                     /locus_tag="VGT87_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEQ3813196.1"
                     /translation="MAKKTKLKKTLVEQILDKANIAHQGLKLNALEGDFPDDLQPSDI
                     YKTLALTGDQTGPLIGIIPLTEHLSEKQLAKVSGNKKVSMVPQKDLQKTTGYIHGANN
                     PVGIRQKHSYPIFIDQTALEKGQIIVSAGEVGRSIKISSQALADFVGASFADLKKRK"
     gene            10495..11118
                     /locus_tag="VGT87_000307"
     CDS             10495..11118
                     /locus_tag="VGT87_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEQ3813197.1"
                     /translation="MKLYFVRHGKTLWNLEGRFQGAGGDSPLLEEAKDEIHLLGKELA
                     KVAFDAVYTSDLQRAMATAAIILDAFDQQPKLYHTDQLREWRLGKLEGAKITTMAAIY
                     PQQMLAFRENLAQFKPDQFEAESIYQTTQRVCHLIQSFKDKHYQNVLIVGHGANLTAT
                     IRSLLGFEPALLLAKGGLDNASLTILETKDYLTYDCLIWNDKSFLEV"
     gene            complement(11226..12128)
                     /locus_tag="VGT87_000308"
     CDS             complement(11226..12128)
                     /locus_tag="VGT87_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEQ3813198.1"
                     /translation="MITAIVFDVDDTIYDQQAPYRIAMEKCFPDFDMSVMNQAYIRFR
                     HYSDVGFPRVMAGEWTTEYFRFWRCKETLLEFGYREIDEAAGVHFQEVYEHELENITM
                     LDEMRMTLDFLKSKNVPMGIITNGPTEHQLKKVRKLGLYDYIDPKRVIVSQATGFQKP
                     EKEIFNLAAEQFDMNPQTTLYVGDSYDNDIMGAFNGGWHSMWFNHRGRQLKPGTKPVY
                     DVAIDNFEQLFGAVKVLFDLPDNKFIFDINDKKNPILQMGINNGLMMAAERLLESNMS
                     IDKVVILLRLTKQQEKVLRLKYAR"
     gene            12302..13795
                     /gene="lysS"
                     /locus_tag="VGT87_000309"
     CDS             12302..13795
                     /gene="lysS"
                     /locus_tag="VGT87_000309"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEQ3813199.1"
                     /translation="MSNQHIEELNDQQIVRREKMMALAEQGIDPFGKRFDRTANSAEL
                     KEKYADKTKEELHELNETAIVAGRLMTKRGKGKVGFAHLQDREGQIQLYVRKDSVGED
                     NYEIFKKADLGDFIGVEGEVMRTDMGELSIKATKLTHLSKSLRPLPEKFHGLTDIETI
                     YRKRHLDLISNRESFDRFVTRSKMISEIRRYLDGLDFLEVETPVLHNEAGGAAARPFV
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADYLDIMNLTEGIIQHAAKAVKGDGPIDYQGTEIRINEPFKRVHMVDAIKEVTGVD
                     FWPEMTVEEAIALAKEKQVPLEKHFTSVGHIINAFFEEFVEETLVQPTFVFGHPVEVS
                     PLAKKNPEDTRFTDRFELFIMTKEYANAFTELNDPIDQLSRFEAQAQAKELGDDEATG
                     IDYDFVEALEYGMPPTGGLGIGIDRLCMLLTNTTTIRDVLLFPTMKP"
     gene            13931..14071
                     /locus_tag="VGT87_000310"
     CDS             13931..14071
                     /locus_tag="VGT87_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813200.1"
                     /translation="MTKEYISYISAAIFLLYGLLAAKWIFLGLGLILVLVGLADRLQS
                     KK"
     gene            complement(14174..14386)
                     /locus_tag="VGT87_000311"
     CDS             complement(14174..14386)
                     /locus_tag="VGT87_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEQ3813201.1"
                     /translation="MAEFTFNIEEHLLTLSENDKGWTKELNRVSFNGAEAKWDIRTWS
                     PDHTKMGKGITLTNEEFKTILDAFRK"
     gene            complement(14596..15882)
                     /locus_tag="VGT87_000312"
     CDS             complement(14596..15882)
                     /locus_tag="VGT87_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEQ3813202.1"
                     /translation="MSHMKKRPEVLSPAGTLEKLKVAIDYGADAVFVGGQAYGLRSRA
                     GNFSMEELQEGIDYAHARGAKVYVAANMVTHEGNEIGAGEWFRQLRDMGLDAVIVSDP
                     ALIVICSTEAPGLEIHLSTQASSTNYETFEFWKAMGLTRVVLAREVNMAELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCVLSNHMSHRDANRGGCSQSCRWKYDLYDMPFGGERRSL
                     KGEIPEDYSMSSVDMCMIDHIPDLIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     MESPEAFYAIKEELIDELWKVAQRELATGFYYGIPTENEQLFGARRKIPQYKFVGEVV
                     AFDSASMTATIRQRNVIMEGDRIECYGPGFRHFETVVKDLHDADGQKIDRAPNPMELL
                     TISLPREVKPGDMIRACKEGLVNLYQKDGTSKTVRT"
     gene            complement(15984..16910)
                     /locus_tag="VGT87_000313"
     CDS             complement(15984..16910)
                     /locus_tag="VGT87_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEQ3813203.1"
                     /translation="MEKIIITATAESIEQVKALLAAGVDRIYVGEANYGLRLPHNFSY
                     DELRQIAKLVHDAGKELTVACNALMHQDMMDQIKPFLDLMIEIAVDYLVVGDAGVFYV
                     NKRDGYNFKLIYDTSVFVTSSRQVNFWGQHGAVESVLAREIPSAELFTLAENLEFPAE
                     VLVYGASVIHHSKRPLLENYYHFTKIDDEVSRERGLFLAEPGDASSHYSIYEDNHGTH
                     IFINNDIDMMSKLGELYAHGLTHWKLDGIYCPGDDFVAITKLFIQAKTLLEAGQFTQE
                     EAEKLDQAVHAHHPAGRGLDTGFYEFDPKTVK"
     gene            17114..17398
                     /locus_tag="VGT87_000314"
     CDS             17114..17398
                     /locus_tag="VGT87_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEQ3813204.1"
                     /translation="MPAPLKKHNSVEQDDSYQESQSKFRDFQEFNGQSAKLKELVFFA
                     RIASFSLITVLFAFVLLVMNLAPMFAFLFASIISLGITSLFAKVIKSLLM"
     gene            complement(17557..17985)
                     /locus_tag="VGT87_000315"
     CDS             complement(17557..17985)
                     /locus_tag="VGT87_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEQ3813205.1"
                     /translation="MNKSFKNLVIGAASGVAAAYFLSTEKGKALKNRAEKAYQAYKES
                     PDDYHQFAKEKGSEYSHLARDTFYDVKDKLASGDLTKEDMLDLLKDKTTAFVQKTKET
                     LAEVEAKEKQDDVIIDLNEEDIIIDYTEQEEPVSDTLDKH"
     gene            complement(17978..18385)
                     /locus_tag="VGT87_000316"
     CDS             complement(17978..18385)
                     /locus_tag="VGT87_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEQ3813206.1"
                     /translation="MDLVGISLMIIALAFVALVIFLIIVLKKVSETIDEAKKTISVLT
                     SDVNVTLHQTNDILAKANILVEDVNGKVATIDPLFVAIADLSESLSDLNSQARHFGQK
                     ATNATGNVSKAGKLALVGKVASKVFGKKGEKHE"
     gene            complement(18407..19186)
                     /locus_tag="VGT87_000317"
     CDS             complement(18407..19186)
                     /locus_tag="VGT87_000317"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEQ3813207.1"
                     /translation="MINPIALKCGPLAIHWYALCILSGLVLAVYLASKEAPKKGISSD
                     AIFDFILIAFPLAIVGARIYYVIFEWSYYVKHLDEIIAIWNGGIAIYGGLITGALVLL
                     AYCYNKVLNPIHFLDIAAPSVMVAQAIGRWGNFINQEAYGKAVSQLNYLPSFIQKQMF
                     IEGSYRIPTFLYESFWNLLGFVIIMMWRRKPKSLLDGEIFAFYLIWYGSGRLVIEGMR
                     TDSLMFLGIRISQYVSALLIIIGLIFVIKRRRQKGISYYQE"
     gene            complement(19183..20115)
                     /locus_tag="VGT87_000318"
     CDS             complement(19183..20115)
                     /locus_tag="VGT87_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEQ3813208.1"
                     /translation="MTVTVKMLVQKVKLNVVYATDNLLSKEITTSDISRPGLEMTGYF
                     DYYAPERLQLFGMKEWSYLTQMTSHNRYSVLKEMFKKDTPAVVVSRNLAIPKEMVQAA
                     KEEGISLLSSRVSTSRLAGEMSYFLDASLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDVYAKDEETLWGEPAEILRHLLEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAIYLENFEAGKVFDRLGNGNEEITFSGVRIPRIRIPVKTGRNVSVVIE
                     AAAMNHRAKEMGFDATKTFEDRLTQLITKNEVSQ"
     gene            complement(20480..20752)
                     /locus_tag="VGT87_000319"
     CDS             complement(20480..20752)
                     /locus_tag="VGT87_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEQ3813209.1"
                     /translation="METKFYKQRKNRLVAGVIAGLADKYGWDLALARVLAALLIYGTG
                     FGVLLYILLAIFLPYKEDLLEERYGRGPRRRKDADVLNEEEDGWFW"
     gene            complement(20816..21253)
                     /locus_tag="VGT87_000320"
     CDS             complement(20816..21253)
                     /locus_tag="VGT87_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEQ3813210.1"
                     /translation="MTLTNYVQEVSLADFGKPFHHKAYWNKRLKTTGGRFFPKDGHLD
                     FNPRMLEEHGELIFRKIVRHELCHYHLYFEGRGYHHKDRDFKDLLAQVNGLRYVSTSS
                     KSKTNHHYSCQTCGQVYQRKRRINLAKYVCGNCHGKLMEKNQS"
     gene            complement(21240..23372)
                     /locus_tag="VGT87_000321"
     CDS             complement(21240..23372)
                     /locus_tag="VGT87_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEQ3813211.1"
                     /translation="MENNNNHNIAEALSVSLHQIEQVLALTAQGNTIPFIARYRKEVT
                     GNLDEVVIKSIIDMDKSLTTLNERKATILAKIEEQGKLTDQLRTSIEATEKLADLEEL
                     YLPYKEKRRTKATIAREAGLFPLARLILQNAQNLETAAEPFVTEGFASPQEALAGAVD
                     ILVEAMSEDAKLRSWTYNEIWQYSRLVSTLKDEQLDEKKVFQIYYDFSDQVSNMQGYR
                     TLALNRGEKLGILKVSFEHNLEKMQRFFSVRFKETNPYIEEVINQTIKKKIVPAMERR
                     VRSELSDAAEDGAIHLFSENLRHLLLVSPLKGKMVLGFDPAFRTGAKLAIVDQTGKLL
                     TTQVIYPVAPASQTKIQAAKETLTQLIETYQIDIIAIGNGTASRESEAFVADVLKDFP
                     NTSYVIVNESGASVYSASELARHEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIG
                     VGQYQHDVSQKKLSENLGFVVDTVVNQVGVNVNTASPSLLAHVSGLNKTISENIVKYR
                     EENGALTSRADIKKVPRLGAKAFEQAAGFLRIPGAKNILDNTGVHPESYPAVKELFKV
                     LGIQDLDDAAKATLAAVQVPQMAETLAIGQETLKDIIADLLKPGRDLRDDFEAPILRQ
                     DILDLKDLEIGQKLEGTVRNVVDFGAFVDIGVHEDGLIHISEMSKTFVNHPSQVVSVG
                     DLVTVWVSKIDLDRHKVNLSLLPPRDTH"
     gene            23606..23761
                     /locus_tag="VGT87_000322"
     CDS             23606..23761
                     /locus_tag="VGT87_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813212.1"
                     /translation="MAITHKKNDELEKMLAGFASIPSFDKPLEVNTDGKLATKEKTTE
                     QTKPSKP"
     gene            23909..24811
                     /locus_tag="VGT87_000323"
     CDS             23909..24811
                     /locus_tag="VGT87_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEQ3813213.1"
                     /translation="MSLFSNLPPSVLQWFAIFMSIIIEALPFVLLGTILSGCIEVFVT
                     PELVQKYLPKQKCLRILFGTFVGFVFPSCECGIIPIINRFLEKKVPSYTAVPFLATAP
                     IINPIVLFATYSAFGNSLRFLILRLVGAALVAITLGVMLAFIVDDNILKDNAQPVHFH
                     DYSHESLPKRIYLALVHAIDEFFDTGRYLVFGTLIASAMQIYVPTRVLTTIGHNPLTA
                     ILIMMLMAFILSLCSEADAFIGASLLSTFGVAPVLAFLLIGPMVDIKNLMMMVKAFKG
                     RFIVQFIGVSVLMIAVYCLLVGVL"
     gene            24811..25626
                     /locus_tag="VGT87_000324"
     CDS             24811..25626
                     /locus_tag="VGT87_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEQ3813214.1"
                     /translation="MIRFLILAGYFELTMYLQLSGKLDQYINVRYSYLAYISMILSFI
                     LALVQLYTWMKNIKVHSHLTGKIARLTSPFILVFPVLIGLLVPTVTLDSTTVSAKGYT
                     FPLAAGASKTGVSDDGTTIQYLKPDTSLYFTKSAYQKEMRQELHKYKGKKPVTITTEN
                     YMEVMELIYLYPDEFLDRDIQYTGFVYNEPGHDNYQFLFRFGIIHCIADSGVYGLLTT
                     GNQTSYPNNTWLTVKGRLHMEYDKNLEQHLPVLQLAEVHQTKEPNNPYVYRVF"
     gene            complement(25725..27149)
                     /locus_tag="VGT87_000325"
     CDS             complement(25725..27149)
                     /locus_tag="VGT87_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEQ3813215.1"
                     /translation="MGIFSKDFLWGGAVAANQVEGAFEADAKGLSVQDVLPNGGLGEW
                     TDSPTSDNLTLEAIDFYHRYKEDIALFAEMGFKVFRTSIAWSRIFPNGDDDQPNEAGL
                     QFYDDLFDELLNYGIEPLVTLSHYETPLHLAKAYNGWTDRRLIGFFERFAQTVMERYK
                     DKVKYWLTFNEVNSILHMPFTSGGIMTEKEKLSLQDLYQAIHHELVASASVTKLAHEI
                     NPDVKVGCMILAMPAYPMTSDPRDILAAHAFENLNLLFSDIHVRGKYPSYIKSYFKEN
                     GIEIVFEDGDKELLAEHTVDFLSFSYYMSVTQAHNPEAYTSGQGNILGGLSNPYLESS
                     EWGWQIDPIGLRLVLNQYYDRYQIPLFIVENGLGAKDQLVQTADGSMTVHDDYRIDYM
                     SQHLVQVAKAIEDGVEVMGYTAWGCIDCVSMSTAQLSKRYGFIYVDRNDDGTGQLTRY
                     KKKSFDWYRQVIQTNGRYLEDNFS"
     gene            complement(27168..29030)
                     /locus_tag="VGT87_000326"
     CDS             complement(27168..29030)
                     /locus_tag="VGT87_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEQ3813216.1"
                     /translation="MTYQETAKAILAAVGGKTNIQRVTHCVTRLRLVLKNDEKVKDQQ
                     VKAISNVIGVMRKNGQYQIILGNDVNNYYQAFLSLGHFDNQDEDHSSKAKGSILERLI
                     ETIAGVITPLIPALLGGGMLKVVGILLTMLGLASADSQTVAFINFFGDAAYYFMPVMI
                     AYSAAARFKVTPVLAATIAGILLHPAFVAMVAEGKPLALFGAPVTPASYGSSVIPILM
                     MVYLMQYIEKWVNRLVPSVMKSFLQPTLIILISGFLALVVVGPLGVIIGQGLSNTMLA
                     IYHVAPWLALAILGAIMPLVVMTGMHWAFAPIFLAASVATPDVLILPAMLASNLAQGA
                     ASLAVAFKTKQKQTRQVALAAGISALLAGITEPALYGVTLKFKKPLYAAMISGGLVGA
                     FIGFVNIASYTFVVPSIIGLPQYINPSGGANFTNALIAGAATIVLAFSLTWFMGIDEE
                     SPEQVSVAADMSQVKSGLSTKQTLYAPMTGEMLFLSEVPDETFSSKLLGEGFAILPSE
                     GVVYAPFDGEVITFFPTKHAVALKNTRGVEVLIHVGIDTVELKGQGFEQLVSVGDVVK
                     RGQALLKMDTDFITSKGYSLISPVVVTNSAEQLEIIIQDDKKMVTKEDALLVIL"
     gene            complement(29032..29874)
                     /locus_tag="VGT87_000327"
     CDS             complement(29032..29874)
                     /locus_tag="VGT87_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEQ3813217.1"
                     /translation="MLIKRVLNHNAAISTNHQGLDILLMGKGIAFGKKVGDSIELNAI
                     ETSFVLKNSDNMNRFTELFITVPQEVVACSERIINLGKIKLGKTLDEILYINLTDHIH
                     SAIERHEQGMLIHNPLRWEIQRYYPDEYSLGVKALELIERNLGVTLAIDEAAFIAMHF
                     VNASLDTPFKEPHRLTEIVSYIEQKIKTDFKTELDDTSIDYYRFMTHIKLFAQRVLSE
                     MSYDDDDAELLLVVKTKYPKEYRCVLDISEEIKKRYNYHLNSSELLYLTVHVKRLVKH
                     LKET"
     gene            30202..31569
                     /locus_tag="VGT87_000328"
     CDS             30202..31569
                     /locus_tag="VGT87_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ3813218.1"
                     /translation="MSHHQQTVSKQTIMAIIAIALIGFSGILSETSMNVTFPTLMSVY
                     QLPLNSLQWMTTIYLLAVAIMMTTSATLKKNVRERPLFFMATGLFTFGTILAVLTQSF
                     AIMLLARIFQGIGTGLVMPQMFNIILERVPMHKVGLFMGFAGLIISLAPAFGPTYGGF
                     MISHFSWQWIFICILPVPLIAGILAYYYLEDSPVSEKVPFDWLAFIALSISLTSALLA
                     ITSLENGSVNLYYLGLFILSFVLFLYKNLTAKQPFLDIRILKIPSLTFGLIPFFVFQL
                     INLGINFLTPNFIVMEKIANSSQAGMVLLPGTLLGALLAPAFGKLYDQKGARLSLYLG
                     NALFSLSLIIMTLQTRHFMLLPFTLLYILFTFGRNMGFNNSLATAIRELPAEKNADAT
                     AIFQMMQQFAGALGTAMASLIANSQPEFTSGVQSVYLLFTIFALLDFIFFFAMFYHLG
                     KKGLA"
     gene            complement(31623..33173)
                     /gene="ftsY"
                     /locus_tag="VGT87_000329"
     CDS             complement(31623..33173)
                     /gene="ftsY"
                     /locus_tag="VGT87_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEQ3813219.1"
                     /translation="MGLFDRLFGKKETPKVAEEKLEENLLTETTQKEELSEKANEQDK
                     IEAVQQEDVSSEGAGSVENGPEAASVNAQVEEETGDNSNHPSEDTNKFAADKTDLKVS
                     ELSQSTASEPKDLVDQPVVEQFPTKQAQADASNDSANEEAVDTSKEQSSSQQVMEDYY
                     RRKAALEKSLQEKAAATVPVMPEEVPQENQASTSAEASQNKATYDTIPETDQEKYKRS
                     LKKTRTGFSARLNSFFANFRRVDEEFFEDLEEMLILSDVGVHVATTLTEELRYEAKLE
                     NAKKPDALKRVIVEKLVDIYEKDGRYNEAINYQDGLTVMLFVGVNGVGKTTSIGKLAY
                     RYKQEGKKVMLVAADTFRAGAVAQLVEWGRRVDVPVITGPEKADPASVVFDGMEKAVA
                     KEVDILLIDTAGRLQNKENLMAELEKMGRIIKRVLPDAPHETLLALDASTGQNALSQA
                     KEFSKITPLTGLILTKIDGTAKGGVVLAIRQELDIPVKFIGFGEKVDDIGEFHSEDFM
                     KGLLEGIL"
     gene            complement(33173..33997)
                     /locus_tag="VGT87_000330"
     CDS             complement(33173..33997)
                     /locus_tag="VGT87_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEQ3813220.1"
                     /translation="MNIRILALDLDGTLYNTEKIVTDANKKALAAVREKGVKVVITTG
                     RPLKAIGNLLEELDLLDHDDYSITFNGGLVQRNTGEVLDKSSLSFDQVCQIQQALEAV
                     GLPTDIISGGDVYSIPSKDGRHSQYHLANPLLTFIEVTSVAELPKDIIYNKIVTVTDP
                     DFLDQQIIKLSPSLFEDFEAFKSRDIIFEIMPKGIDKAFGLNLLCQHLGLDARHVMAM
                     GDEANDFAMLEWAGLGVAMANGVSGAKAVADAVTTLTNDESGVAEAVKTFILEEES"
     gene            complement(34001..34798)
                     /locus_tag="VGT87_000331"
     CDS             complement(34001..34798)
                     /locus_tag="VGT87_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEQ3813221.1"
                     /translation="MIKLIATDMDGTFLAEDGTYNQEQLAALLPKLAEKGILFAVSSG
                     RSLLAIDQLFEPFLDQIAVIAENGSVVQYRGEILFADMMTKEQYTEVAKKILANPHYV
                     ETGMVFSGQKAAYILKGASEEYIQKTKHYYANVKVINGFEDMENDAIFKVSTNFTGHT
                     VLEGSDWLNQALPYATAVTTGFDSIDIILKEVNKGFGMEHLCQALGIKKAETIAFGDN
                     FNDYQMLEFAGRAIATENARPEIKVISDQVIGHCNDGAVLTYLKGLV"
     gene            35103..36230
                     /locus_tag="VGT87_000332"
                     /pseudo
     CDS             35103..36230
                     /locus_tag="VGT87_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT        10934 a         7731 c         6488 g        11125 t
ORIGIN      
        1 ttgcttgata tttacaactt tactattttc ttttcgagtg tcctttcgat aagataattc
       61 agcttcttta aatatatcat ctcgcttttc gctagcatca atatttagta caaaacctac
      121 tgcaacagat aagacaagta agctatttcc accttgagac aaaaaaggga aggtaacacc
      181 agtagatgga attaagcctg agataccacc aatattaaca aagacttgca tgagcatcat
      241 accgcctact cctaaagcca tcatggcatt aaaaggattt tttgctttaa taccaacatt
      301 cataatacgt aaaattaaga agaaaacaag tgccaaaata aaccctgctc ctattagacc
      361 taactcttca atgacaactg aaaagacaaa gtctgtctgt gcttcgggca aatagccacg
      421 cttttcaatg gaatttccta acccttggcc aaaccaccca ccattactca tagcatagta
      481 agaattggct aattggtgac ctgaatcggt caagtcatga aaaggattaa aaaaggcact
      541 aaaacgttta gcgacatagc caaaaacagg aatcttagca actctttcta ccccaataac
      601 ggcaatagtc ccaagaaaga ccgtggatag accggtaatc atgactagga tagccgaaaa
      661 ccatcggtaa ccaatgccac tgatagaaaa cataataatg gctgtcaata caataatgga
      721 agcatttccc aagtcaggct gagcagcaac caaaagtacc atcaacaaag aataaacacg
      781 ccagtcccgt aaatcgttcc attgtgttgg ccaccatttt cgacgagtta gtgcttgata
      841 atcataaagg ctaatgtttt tttggatctt agcaaaggtc aatgccaaat accataccat
      901 aatgatcttt aaatactcag caggctgaaa gctgactggt ccgatcacaa tccaaccatg
      961 cgctcctttg atagcagttg taaagaagcg ggcaataatt aaaagaaagg cctctcctaa
     1021 cataaccaca gttaataccc ttgtattcgt taaaaaattt aattttaatt tatagataaa
     1081 tgtaatagcc actagactta ttatccaaaa aactccctgg ttaatcaccg atttaaatgg
     1141 attagcatgt gcttgaatca agctgacact tgtggttgag taaaccatta ttaagcctat
     1201 caccgataaa attaaataag gcaataatat agaataattt aagaggtgcc ttttatcaat
     1261 cttcatagtt ttccctctac ttcttgagat aatcatttag cattataaca tctttctata
     1321 tcaaacaaaa ggaaaagtca agaatcctca aaaaaagaag ctccaagggc taacctttaa
     1381 aattctgatt agtcacttga aaacttctat aaataccaat taatactcaa gggcattagc
     1441 ctgaattacg taaaccagtc gctatgccat tgatagttgt atggatcaat ttttcatcat
     1501 tttcgtcaag ttgattatta cgccaacgtt taatcaattc aatttgaata tagttcaaca
     1561 cattaaaata cggcaagcgt gacttcaagc tatgcttaag ggaaggatta tcctccaata
     1621 gatcatcatg gtcttggata gcaagaataa cattttttgt caattgccac tcatccaaaa
     1681 tctcataaaa aacatctcga acatcctgac tttcagctag ttgtgcatac tgaaaggcaa
     1741 tattcatgtt tgatttagat aataccatat caacatttga taacaaggaa tggaaaaaag
     1801 gccacgtctg atacatgtgt tggaggcgtt caaggttact ttgtgctcgg tcaatataac
     1861 gtttaaaggc tgaacctacc ccataccaac ctgggaacat gatacgattt tgtgaccatg
     1921 aaaagaccca aggaatggca cgcaagcctg taatttccgt aatcgtttta cgggctgctg
     1981 gtcttgaccc aatgttgaga ctggaaactt ccttaatcgg gcttgcctca aagaaataat
     2041 catagaaatg gggattgtca aaaaccaatt ttcgataaat ggtattgcta tcagcaacaa
     2101 tgctatccat gatttctcga aaactatcaa tctcattagg atctgttatc atttgagtca
     2161 ccatacgatt gatagaagct gaaatcaaca tttctaaatg gtaataagcc acatctttat
     2221 tgccgtattt gttttcaata atttcccctt gctctgtcag acgaatccga tctttgatgg
     2281 aaccaaaagg ttgtgaagta atggcatcat aagaagggcc acctcctcga ccaactgttc
     2341 ctccacgacc atggaagaag gtgattttca ctccatgttc ctcaccaata gctgttaatt
     2401 cattctgagc tttataaaga gtccaacccg atgccaagta gccaccatct ttattactgt
     2461 cagaatagcc taacatgatt tcttggtaat tacgattcgt tgcaatccaa gatttgacaa
     2521 tgtcatggct taaataggct gccatgatgt cacgggcatt atctaaatcc tcaatggttt
     2581 caaagagggg aacaatttgc acacgagccc gctgctggtc aaccaatccc acttctttaa
     2641 gcataatggc caactcaaac atatcagaga cactttctgt atgtgaaata atatgttgct
     2701 taataacctc ttctccaagc ttatctttca attctcgtgc cgtagtatag atagccagtt
     2761 ctttttctaa taggtctgat ttagcaactg ccgctgagga taaggttctt ggctcctcca
     2821 ttaattgttg taagagaaca tcgcattttt cagtttctga aagagagcta taatcgtcaa
     2881 caatattggc tccttttaat aactctgcca cacaagcttc ttggacactg gagtcttgcc
     2941 tcatgtcaat gcttgctaag aagaagccaa aaatatcaac agcttgcatg acttctcgca
     3001 aatccccctc aatcaaagcg gagttgccat tatcaagtag cgactgctca atagccttta
     3061 aatcagcttt aaaatcaaca ggagagtcat aggctaagat gggtttttct aaactagctg
     3121 gtgagggcca agcagatgtg tttagtccca cactactatt catacgagct gctggctgat
     3181 tagttagttg aatttgtgtt tgtttaagac gcgattgaat ataatgaaaa gctctgcgat
     3241 aaggttcatt tccacggtaa atcgattggt cctgagacaa actggctaaa cgttccactt
     3301 ccgagttggg ttgtaccaag gttgacgaca gtgaaaatgt tcggtaaagg gctgctaatt
     3361 tgtcaatgta gtaattgagg ataacctcac tttgaaccgt agctgacaag cacaaggttt
     3421 cagctgtgac aaaggggttg ccatcacggt ccccaccaat ccacatcccc atggtaatgg
     3481 gtttgggatt gtctaactct agaccgtgtt tttgggctaa gtttttgtac tctgttgtca
     3541 acttggtcac tgcttgaatg agagaaccat cgtaatactg catgacattt tttatctcgt
     3601 ttttcacctg aagctttttc tcgcgaataa tatctgtctg catgatcatt tcaatataac
     3661 gatatagctc ctgacgccat ttctcaagat taatcacacc agccttagca tcacggtatt
     3721 tgcgcagaag tttatgaatg tgagttgtca attccagaat cgttttacgt tgtacttgcg
     3781 tcggatgtgc tgttaaaaca ggaaccacat taacctgctc caaaatgtct ttaccattat
     3841 cttttccagc aagatccttg atggttaggg ctaattttcc taaataatca gtatccgtat
     3901 tattttgatg attaatttca taggctaaat cgacatcttc tgaaatattg attagcaagg
     3961 gtaaaataga aaaatacctg gaaatcactt ccatttcttg atttgaaata ttggcgacaa
     4021 ctttttctaa ttctatgtaa tcttgttttt cagataacac cataatagac tcgataactg
     4081 taaacgcatc atctccaatc atccgtcgtg tgatattttc caacatttct ttcaaaagag
     4141 caacttcttc agcaatgata gtttgattgt tactactctc taacttttta agtggcaaat
     4201 gaccatctcc ttctttgcta gtttcttcta tcataccact ttttaccaaa aataaaagtg
     4261 agcatgttca taacgtttgt tttttaacaa aagtaagcgc tttatttcaa aaaatgtttg
     4321 gataggttta gtctccatat tccactaaaa ttctgttata ctagtcttga ctatattgtt
     4381 aaggagacaa ctatggaatt gaaaaaacgt tcagaattcc ctgaaaatga actttgggat
     4441 ctaacagcgc tctacaaaga ccgccaagat tttttactcg ctattgaaga agccttacaa
     4501 gatattgacc tgtttaagcg caattatgaa gaccgcttaa catcggtgga cgatttcact
     4561 caggccttaa tagaaattga gcatatttat actcaaatga gtcatattgg cacttatgcc
     4621 tttatgcctc aaaccactga cttttcagat gaaagttttg ctcaaattgc tcaggctggc
     4681 gatgatttca tgactaaagc tagcgttgcc cttagcttct ttgacaccgc cttagccaat
     4741 gccgaccttg acgtgttaga tacattagaa aagaaccctt atttcagcgc agctattcgc
     4801 atggcaaaaa tccaaaaaga acacctctta agtcctgatg tggaaaaggc tttggctaac
     4861 ttacgcgagg ttatcaatgc gccctatgat atttacacca aaatgcgggc tggtgacttt
     4921 gacatggatg attttgaggt ggatggtaaa acctataaaa atagctttgt ttcttacgaa
     4981 aatttctatc aaaatcacga aaatgctgaa attcgtgaca aagctttccg ttccttctca
     5041 aaaggtctcc gtaaacacca aaatactgct gctgctgctt atttggccaa ggttaaatct
     5101 gaaaaattgt tagctgatat gaaaggctac acttctgtct ttgattacct cctagctgaa
     5161 caggaagtgg atcgtagctt gttcgaccgc caaattgacc tcatcatgac tgaatttgga
     5221 ccggttgctc agaaattctt aaaacatgtc gcccaagtca atggtcttga gaaaatgact
     5281 tttgctgact ggaagctaga tattgacaat gatctcaatc cagaagtcag cattgatggt
     5341 gcctatgacc tcgtgatgaa gtctttagcc ccattaggac aagaatacac gaaagaaatc
     5401 gaacgttatc agacagaacg ctgggttgac tttgctgcta atgccaataa agattctggt
     5461 ggttatgccg ctgatcctta caaggtccat ccgtatgtct taatgagctg gactggcaga
     5521 atgtctgatg tctacacgct cattcatgaa atcggacact ctggccaatt tattttctct
     5581 gataatcacc aaagttactt caacacccat atgtcaactt attacgtcga agcgccatcg
     5641 acctttaatg aattgatgct tagtgattac ttggaacatc agtttgatga ccctcgtcaa
     5701 aaacgttttg ctcttgctca ccgtttaaca gacacctact ttcataactt catcactcac
     5761 ctattggaag cggctttcca acgtaaggtt tacaccctga ttgaagaagg cggtaccttt
     5821 ggagctgacc aattaaatgc aatgatgaaa gaggtcttaa cagacttctg gggagatgcc
     5881 gttgacattg acgatgacgc agctcttacc tggatgcgcc aagcgcatta ctacatgggc
     5941 ttatatagtt atacctattc tgctgggctt gttatgtcaa cagctggtta ccttaacctt
     6001 aaaaacaacc cagatggggc aaaagaatgg cttgatttcc taaaatcagg tggtagccgt
     6061 acgcctcttg atactgcaat gctcattggg gcagacattg ccactgaaaa gcctcttcga
     6121 gataccattc aattcttgag tgataccgtt gaccaaatta tcaattacac cgaggagatg
     6181 tcacatgcct aatctctatg attttacggt taaggctcag gacggcagtg acatctcact
     6241 agttaactac aaagaaaaag tggttttggt cgtgaataca gctaccaaat gtgggctaac
     6301 cccacaatac caggccttgc aagcactata tgacacatac catgacaagg gatttgaggt
     6361 cttagatttt ccttgcaacc aatttttaaa tcaagctcca ggagatgctg aggagattaa
     6421 tcacttttgc agtctgacct atcacactac tttccctcgc tttgcaaaaa tcaaagtgaa
     6481 tggtaaagac gctgatcctt tgttcacctg gttaaaagaa gaaaaaagtg ggcccctcgg
     6541 gaaacgcatc gaatggaact tcgctaaatt tctcattgac caaaatggtc aagtcattaa
     6601 acgctattcc tcaaagacag atcctaaact aattgaagaa gaccttaaag cacttcttgg
     6661 ttaatataaa atagacttgg tgtgccaagt ctattttata ttatagttac taattaaaaa
     6721 cttcttgata aaattcaatg ctatcaccat ctttaacggt aatttgattg gcacccttat
     6781 ctgccatctt tccattcacc ttaaaaagcc agtatttgtt agctttcata tcttgttcaa
     6841 taccatcaat agcagtgata aagccatctt tttctttgac ctcataatta tctttgagaa
     6901 tctcaagcac tgtgtctcct tcctcaaaag aaactttttc gtcaacagta ttggtgtctt
     6961 ctttaacaac taagcgaacg tgatggtctg atttgggaac gcttggagtc tgtgtttgtt
     7021 ttgttccttg cgagcaagcc actagtagca tagcaacaag tgctaaaaat cctattttaa
     7081 atcctttgtt caccgattaa cctcctaaat aacatcataa taactggaaa aaagacgaga
     7141 gttgataaag catgcgctat attaaagggc atccctgcta aaacatagct ccaccaaggc
     7201 atactatata aatacgcaaa acaagtatct atcagcaccc cataaaggag gccacataag
     7261 gcgaccaaga aagcctgaca gccaagctga tatttgggga attgctgaga caaagggtac
     7321 aagacaaaac gccataacaa aagaacaagc acaaaacaag taacttgcca aaagacccaa
     7381 gggccaaatc ccagcaaaaa ggctgataaa aacaagcaca acatcataac taatacagcc
     7441 tcggctaacc caaagtaaag aagataactg agtaaaaatg ccgtcacagg ctgcacgtta
     7501 ggcaggctgc taaaaaccat tctcaacaca acacataggg ctgacatgat agcaatgcga
     7561 ctcataagct tagctgacat aaacttcttc ctcgtttttt tctctattat gacagattta
     7621 caaagcgttt accagtttgg agctcactta tttatcaaat gataataaca tgagaattaa
     7681 ataacaaaac agtacttgct gactgtcatg atatagtcaa agactaaact aatattgtaa
     7741 agaatatagc taccaactat aagagatagt gacataaact tgacatagtt agagaggatc
     7801 tcatttgtaa cttctcaaag agaatacctt aaaatgcaag acaatataag aaaacagtta
     7861 ggatattttg atttttttta gtaattaggg tacaataaca gttaatgaga agaaaaataa
     7921 aacctattgt tgtgctagtc ttctttattt tactggcaat ggtattgatt atcggaaaaa
     7981 gacaagctaa tcatgcgaaa caaaaagaag ttgaagacgc taagagtcat attcctattg
     8041 caaccagtaa tcctagtaaa gcaaaagcta gcacttctga aaccgaagac tttattttaa
     8101 atcctattgt tgatgtttcc ggctggcaac tccctgaaga aattgattat gacacattgt
     8161 cacgtcacat ctctggcgca attgtacgtg tctatggagg atcgcaaatt acagctcata
     8221 acaacgctgc ttttacaact ggaattgaca aatcttttaa aacgcatatc aaagaatttc
     8281 aaaaacgaaa tgttcctgta gctgtttaca gttacgcact tggtcgtagt actaaagaga
     8341 tgaaagaaga ggcccgagct ttctacaaaa acgctgctcc ctataatcca acttactact
     8401 ggattgatgt cgaagaagcc actatgaaag atatgaataa aggcgtaaca gccttccgag
     8461 aagagcttaa aaaacttggt gctgaaaatg ttggtctcta tattggaact tattttatgg
     8521 cagaacaaga tatttcaaca aaaggtttcg atgctgtatg gattccgact tatggtagtg
     8581 attctggcta ttatgaggct gctcctaata caaccttaga ttacgattta caccaatata
     8641 cctcacaagg ttatctcagt ggttttaaca atgctttaga tttaaatcaa attgccgtaa
     8701 caaaagacac taaaaaaacg tttgagaagt tatttggcaa ctccaacaat taagaggtta
     8761 gcatcatggc taatcttttt tgtagtttac atcttaccaa gaaaaactct aggtcaataa
     8821 tttaaccccc cttatccttg acaaggagat tatctcatca tataatgaaa gcactataat
     8881 atttcacaaa ggagtgcttt ggctgagatc gcaattgcga aatcctgagg acctgatctt
     8941 gttagtacaa gcgtagggat tgtgaccaat aatcaatgat tggaaagcga tactcttttt
     9001 gtgcacaaaa ggagtttttt atgtcaccaa acaccaatgt caaatacctt atcgaagctg
     9061 ctatttttgc tgcattggcg atggcccttt catttattcc agattttgct ggctggttta
     9121 gtccttctta tggtgctatt gcactggtta ttttctcttt gcgccgtggg ttaaagtacg
     9181 gcatgttggc gggtctcatt tgggggctgc ttcactttgt acttggaaaa gtttactatc
     9241 tcagcatgtc ccaagtcttt attgaatata tcttagcctt tacctctatg ggattagccg
     9301 gtagtttctc tgatagtttg attaaaactt taagaagaca acaaaccttt tttgccgttt
     9361 ttcttgcaat aatggctagt ttactagccg ttacagtacg ttacttgtgg cacttccttg
     9421 caggaatcat tttctggggc agttacgccc ctaaaggcat gtccgctgtc tggtattctt
     9481 ttagcgttaa tggtacagct ggtgtcctaa cttttttaat aacatgttta gcgttaatga
     9541 ttgctttacc aattcatcct caattatttg atcccaaaga ctaattttta gtcatttgaa
     9601 tcttacatta aaaaaatggt ataataatgt tgttatacca tttttttatg tttagataaa
     9661 ggtaaggact tgatatggct aaaaaaacaa aactcaaaaa aaccttagta gaacaaatcc
     9721 tcgataaggc taatattgct catcaaggac tcaagcttaa tgctttagaa ggcgactttc
     9781 cagatgacct tcaaccatca gatatttaca agacacttgc tctgactggt gatcaaacag
     9841 gtcctctaat tggcattatt ccattaacag aacacttatc agaaaaacaa ttggcaaaag
     9901 tatcaggtaa caaaaaagtg tccatggttc ctcaaaaaga cttgcaaaag acaacaggct
     9961 atattcacgg tgccaataat cctgttggga ttcgtcaaaa acattcatat cctattttta
    10021 ttgaccaaac tgcactggaa aaaggtcaaa taatcgtttc agctggtgaa gttgggcgtt
    10081 ctataaagat ttctagccag gctctagctg atttcgttgg cgcaagcttt gctgatttaa
    10141 agaagagaaa atgaacacta ctgccaactt tatctggttt cttttttaag tttttcgcct
    10201 tgattaagct agtctttgtt tagtattatt ttgatctcaa ctgctttaac attgatcctg
    10261 ttaatctttt tcttttgtag agacttgata tcacgacaaa ttcaggtcga tttgtcttag
    10321 atctaatatg ggctttacac tttaatcaga acctagctat tatacactta tactacgctt
    10381 ttttacttta tcttttcact ataggtatta tttttgcttg gtgatgccta aactttttct
    10441 ggttactttt ttatttttag cgctcgcatc agttatctct aaaaggaggc ttaaatgaaa
    10501 ttatattttg taagacatgg gaaaaccctt tggaatttag aaggtcgctt tcaaggcgct
    10561 gggggcgatt cgccattact tgaagaggca aaagacgaaa ttcacttact tggcaaagag
    10621 ttagctaagg tggcttttga tgccgtttat actagcgacc ttcaaagagc tatggcaaca
    10681 gcagctatta tcttagatgc ttttgaccaa cagccaaaac tttaccacac cgaccagctt
    10741 cgggagtgga gattaggtaa actggaaggg gctaaaataa caacaatggc agctatatat
    10801 ccccaacaaa tgctggcttt tcgggagaat ctagcccagt tcaaaccaga tcagttcgag
    10861 gctgaatcaa tttaccaaac gactcaacgt gtctgccatc ttatccaatc gtttaaagat
    10921 aaacattatc aaaacgttct tattgtagga cacggcgcta acttaacagc aactattcgc
    10981 tcattattag gttttgaacc tgctctttta ctcgctaaag gtggcttaga taatgccagt
    11041 cttactattc ttgaaactaa agattattta acttatgact gtttaatttg gaatgataaa
    11101 tcttttctag aggtgtaaaa ccactagaca ataataaaaa aagctaacac attgatttct
    11161 tactgacaag acactcactt tgctagcttt ttttattatt atctaatggg ctattatggt
    11221 ttcatctatc tggcatattt taagcgcaaa actttttctt gctgcttggt taaccgaagc
    11281 aagataacca ctttgtcaat gctcatgtta ctttcaagta aacgctctgc tgccatcatc
    11341 aagccgttgt tgatacccat ctgtaaaatt ggatttttct tgtcattaat atcaaaaata
    11401 aatttattat caggtaaatc aaagagtact ttaacggcac caaagagctg ttcaaagtta
    11461 tcaatagcca catcataaac aggcttagtg cctggtttta gctgacgacc tctatgatta
    11521 aaccacatgg aatgccaacc accattaaag gcacccatga tatcattgtc ataagagtcc
    11581 ccaacatata gtgtggtttg tgggttcatg tcaaattgtt ctgctgccag gttaaaaatt
    11641 tctttttctg gtttttgaaa gcctgttgct tggctcacaa tgacacgctt aggatcaata
    11701 taatcataaa gccctaattt acgaactttt ttcagctgat gttctgttgg cccattggta
    11761 ataatcccca taggaacatt ctttgatttt aaaaagtcaa gggtcatgcg catctcatct
    11821 aacatggtaa tgttctcaag ttcgtgctca tagacttctt gaaaatgtac tcccgcagct
    11881 tcgtcgattt ctcgatagcc aaactcaagc agtgtttcct tgcaacgcca gaatcggaaa
    11941 tattctgtgg tccactcccc tgccattact cttgggaatc caacatctga atagtgacga
    12001 aagcgaatat aagcttgatt cattactgac atatcaaaat ctgggaagca cttttccatg
    12061 gcgattcgat aaggcgcttg ctggtcgtaa attgtgtcat ccacatcaaa aacaatagca
    12121 gtaatcatga gtctcttttc ttttctagtt taatatccca atcattatac tattttccat
    12181 ttggcttttc aattttattt tcaaaaaagt gccccgctgc agtcgcattt tccaacgaaa
    12241 tataggtgta ttctagcctc ttttatgata caatggaaag gaaatcgtat tggaggaaaa
    12301 tatgtctaat caacacatcg aagaattaaa tgaccaacag attgtccgtc gtgaaaagat
    12361 gatggctctg gctgaacaag gaattgatcc tttcggtaaa cgctttgacc gcacagccaa
    12421 ttctgctgaa ttaaaagaaa agtatgctga taaaacaaaa gaagaattac atgagttaaa
    12481 cgaaacagct attgttgctg gccgcttgat gacaaaacgt ggcaaaggta aggttggttt
    12541 tgctcatctt caagaccgtg aaggccaaat ccaactttat gttcgcaagg actctgttgg
    12601 cgaagacaac tatgaaatct tcaaaaaagc cgatttaggc gactttatcg gcgttgaagg
    12661 cgaagtcatg cgtacagaca tgggcgagtt gtcaatcaag gcaaccaagt tgactcacct
    12721 ttctaaatca cttcgtcctc ttcctgaaaa attccacgga ttaactgata ttgaaaccat
    12781 ttaccgtaag cgtcaccttg atttaatttc aaatcgtgaa agctttgacc gcttcgtgac
    12841 ccgttccaaa atgatttcag aaatccgtcg ttatttagac ggtcttgatt tcctagaagt
    12901 agaaacgcct gtgcttcata acgaggctgg tggtgctgct gctcgtcctt tcgttactca
    12961 ccataatgct caaaacattg atatggttct tcgtatcgca acagaacttc atttgaaacg
    13021 tttgattgtt ggtggtatgg aacgtgtcta tgaaattggt cgtatcttcc gtaacgaggg
    13081 aatggatgct actcataatc ctgagttcac ttctattgag gtttaccaag cctatgcaga
    13141 ctatcttgat atcatgaacc tgacagaagg cattatccaa catgctgcta aagctgttaa
    13201 aggtgatggc cctattgatt atcaaggaac tgaaatccgt atcaatgaac ctttcaaacg
    13261 tgtccacatg gtagatgcta tcaaggaagt gacaggtgtt gatttctggc ctgaaatgac
    13321 tgtcgaagaa gctattgcac ttgccaaaga aaaacaggtc ccactcgaaa aacacttcac
    13381 aagtgtcgga catatcatca atgctttctt cgaagaattc gttgaagaaa ccttagtcca
    13441 accaaccttt gtgtttggtc atcctgttga agtatcgcct ttggctaaga agaatccaga
    13501 agatacccgc ttcaccgacc gcttcgaact tttcatcatg accaaagaat acgccaatgc
    13561 tttcacagag ttgaatgatc ccattgacca attatctcgt tttgaagcac aagcacaagc
    13621 caaagaactt ggtgatgacg aagcaactgg cattgactac gacttcgttg aagctcttga
    13681 atatggtatg ccgccaaccg gtggactcgg aatcgggatt gatcgtcttt gcatgttatt
    13741 aacaaacaca acaacaatca gagatgtgct tttattccca acgatgaaac cttaactttt
    13801 ttaagatttg tattcatatc ttaaaaccga taagtggcaa tataacataa cgttttatta
    13861 tctctaacca gtcttcatta ccatgaagac tggtttatta acagtagctt atagaaagtg
    13921 acttaacaag atgaccaaag aatatatctc ctacattagt gctgctatct tcttgcttta
    13981 tggcctttta gctgccaaat ggatttttct tgggctaggc ttaattttgg tacttgtcgg
    14041 ccttgctgac cgtcttcaat ctaaaaaata acccttctcc gtgtcaactg cacccgatta
    14101 ccagccttaa aatggccaag atgtcaaggt gcagttgact agaagggtta ttttttacaa
    14161 ccacagagag gttttactta cgaaaagcgt ctaagatggt tttaaattct tcattagtaa
    14221 gagtaatgcc tttccccatt ttcgtatggt ctggactcca cgtgcggatg tcccatttcg
    14281 cttctgcgcc attaaagctg acgcggttca attctttcgt ccaaccttta tcattttctg
    14341 ataaggtgag taaatgttcc tcaatgttga atgtaaattc tgccattagt tttcctcctc
    14401 atctatacta ttcgaaatta aagtggaaaa aatcatcttt ttttcacttt ttttatgact
    14461 cattctccta ttattctatc aagttttgag aaaataagct attgtgatat ccaattttca
    14521 aaaaaaacag gctctctttt cccaactatg gtcgtatacc atttggaaaa agagggcctc
    14581 tagtatttct tgttactatg ttctaacagt tttactggtg ccatcttttt gatagaggtt
    14641 gacaagacct tccttgcaag ccctaatcat atcccctggc ttaacttctc tcggtaaaga
    14701 gatggttaag agttccattg gatttggggc acggtcaatc ttttggccat ccgcatcatg
    14761 taagtcctta acaaccgttt caaaatgacg gaaacctggt ccataacatt caatccgatc
    14821 gccttccatg atgacattac gctgacgaat ggtcgctgtc atgctggctg agtcaaaggc
    14881 aactacttct ccgacaaatt tatattgtgg aattttgcgg cgagcaccaa ataattgttc
    14941 attttcagtt gggataccat agtaaaaacc tgtagccaac tcgcgctggg caaccttcca
    15001 caactcgtca atcaattcct ctttgatagc ataaaaagct tctgggcttt ccatgtaagc
    15061 atctacagcc gccttgtaac agttggttac ggttgagacg tagtggatag atttcattcg
    15121 gccttcaatt tttaagctat caaccccatt ttcaatcagg tcaggaatat ggtcaatcat
    15181 acacatgtca acagaggaca tagaatagtc ttctggaatt tcccctttta aggagcggcg
    15241 ctctcctcca aatggcatgt catacaaatc atacttccag cggcaagact gtgagcagcc
    15301 gcccctgttg gcatcacggt gactcatgtg gtttgacaaa acacagcggc ctgaataaga
    15361 gatacacatg gctccatgga caaaggcttc aatttccaca tctgtccgct tacggatttc
    15421 tgctaactcg gccatattaa cctcacgagc taaaacaact cgggtcaaac ccatggcttt
    15481 ccaaaattca aaggtctcgt aattggtaga tgaagcttgc gttgacaaat gaatttccaa
    15541 acctggggct tctgttgaac aaataacaat caaggctgga tctgaaacaa tgaccgcatc
    15601 aagccccata tcacgcagtt gacgaaacca ctcgcccgca ccaatttcgt tcccttcgtg
    15661 ggtaaccatg ttggcagcaa catagacctt agctccacgc gcatgtgcat aatcaatgcc
    15721 ttcttgcaat tcttccatag agaagttacc agcgcggctt cttaggccat aggcctgtcc
    15781 tccaacaaaa acagcatctg cgccatagtc aatcgcaact tttaattttt caagtgttcc
    15841 agcaggtgat aagacctcgg gacgtttttt catatgtgac atgtgatatt ctttctattt
    15901 tcaagatagg tagattacct gtgtaatggt gtcataaccc tgtcttgccc tgaaatagta
    15961 aactcactca agacaaggat tacttattta acggtttttg gatcgaactc ataaaagcca
    16021 gtatctaatc cacggccagc tggatgatgg gcatgcactg cttgatcaag cttttcagct
    16081 tcttcttggg taaattgccc agcttctaat aaggttttag cctgaataaa caatttagta
    16141 atagcgacaa aatcatcacc tggacagtaa atgccatcta gcttccaatg agttaagcca
    16201 tgcgcataca gctcacccaa tttagacatc atgtcgatat cgttattaat gaaaatatga
    16261 gtgccatgat tgtcttcata aatggagtaa tggctgctag catctcctgg ttctgccaag
    16321 aaaagtcctc gttcacgact aacctcatca tcaatctttg taaaatggta ataattttct
    16381 agcaagggac gtttcgagtg atgaatcacc gaggcgccgt aaactagcac ctctgctgga
    16441 aattccaaat tttcagctaa ggtgaataac tctgctgatg ggatttccct tgctaaaaca
    16501 ctttcaacag caccatgttg cccccagaaa tttacctgac ggctagaagt cacaaaaaca
    16561 gaggtatcat aaatcagttt aaagttatag ccatcccttt tattgacata aaacacacct
    16621 gcatcaccaa ccacaagata atcaacagca atctctatca ttaagtctaa aaagggtttg
    16681 atctgatcca tcatatcttg atgcatcaaa gcattgcaag ctactgtcaa ctctttgcct
    16741 gcatcatgca ctagtttggc aatttgtctc aattcatcat aagaaaaatt atggggaagg
    16801 cgtaacccgt aattggcttc accaacataa atacgatcaa caccagcagc taataaagct
    16861 ttgacttgct caatgctttc agctgtggct gtaataatta ttttttccat ataagcttta
    16921 ttataccaaa aattttaaga ccgtaagcat ctttcttgca aaagcccaag cattaagcta
    16981 gtccccttac tgttatagca tgataagtca cttgtcatta gtaaatcttt tcaaatcttt
    17041 taaaaaaaga tgacaagaag agacttttat gatagaatta cttagtatgt taaggaaagg
    17101 agaggagagg actatgcctg caccgttaaa gaagcataat tcggttgaac aggatgattc
    17161 ttaccaagaa tctcaaagta aatttcgaga ttttcaagag ttcaatggcc aaagtgcaaa
    17221 gctaaaagaa ttagtcttct ttgctcgtat cgctagtttc agccttatta ccgttttatt
    17281 tgcatttgtc ctgctcgtaa tgaatttagc gccaatgttt gctttcttat ttgcaagcat
    17341 cattagctta ggcattactt ccttattcgc caaggttatc aaatcattac tgatgtaact
    17401 tccagagtcc gttttacgtg actctttttt tgtgtcaaaa acaaaaaaag attgaagcca
    17461 caataccccc cttcttcagt tagcctaaaa ctaaccatcg ggactagtga tcagatcctt
    17521 caatcttttc ttcttctatg ttttaggtca ttttagttag tgtttgtcta atgtatcact
    17581 aacaggctcc tcttgttcag tatagtcaat gatgatgtct tcttcattta aatcaatgat
    17641 aacatcatct tgcttttctt tcgcttcaac ttcggcaagg gtttcttttg ttttttgcac
    17701 gaaagctgtt gttttatctt tgagcaagtc taacatgtcc tctttagtta agtcaccaga
    17761 agccaactta tctttcacat catagaaagt gtcacgtgct aaatggctgt attcactacc
    17821 tttttcttta gcaaattggt ggtaatcatc tggactttct ttgtaggctt gataagcttt
    17881 ttcagcacga tttttcaaag ccttgccttt ttctgttgat aagaagtaag ctgccgctac
    17941 tcctgaagca gctccaatca ctaagttttt gaaactttta ttcatgcttt tctccttttt
    18001 ttccaaatac ttttgaggct actttaccaa ctaatgctaa ttttccagct tttgaaacat
    18061 tacctgtagc attagttgct ttttgcccaa aatgccttgc ttgactattt aaatctgaaa
    18121 gactttctga caaatcagca atagcaacaa acaatggatc gatggttgct actttaccat
    18181 taacatcttc aacaaggata ttagcttttg ctaaaatatc attagtttga tgaagtgtca
    18241 cattaacatc acttgtcaat acagaaatcg tttttttggc ttcatcaatt gtttctgaga
    18301 ctttctttaa tacaataatc aagaaaatca ccaaagcaac aaatgcgagt gcaataatca
    18361 tcagtgagat tccaactaag tccataagtt tctccttttt ctatccttat tcttgataat
    18421 aagaaattcc tttttgacga cgtcttttta taacaaagat taagccaata ataattaata
    18481 aggcagacac atattgggag atgcgaatac ctaaaaacat aagactatct gttcgcatac
    18541 cttcaatgac tagccttcca ctaccatacc aaattaagta aaatgcaaag atttctccat
    18601 ctaataggct ttttggctta cgacgccaca tcataataat gacaaatccc aaaagattcc
    18661 aaaatgattc atagagaaag gtcggaatgc ggtaacttcc ctctataaac atttgctttt
    18721 gaataaagct tggtaagtaa tttaactggc tcacagcttt accataggct tcttggttga
    18781 taaaatttcc ccagcgaccg atagcttgag cgaccatgac acttggtgcg gcaatatcta
    18841 aaaaatgaat gggattgagc accttgttat aacagtaagc caaaagtaca agagctcctg
    18901 taatgagacc gccataaata gcaataccgc cattccaaat ggctatgatt tcatctaaat
    18961 gcttgacata ataagaccat tcaaaaataa catagtaaat tcttgcacca acaatagcaa
    19021 gtggaaaagc aatcagaata aaatcgaaaa tagcgtcaga tgatattcct tttttgggtg
    19081 cttcttttga tgctaagtaa accgccaaaa caagtcctga caagatacat agcgcatacc
    19141 agtggatggc tagtggacca cattttaagg caatgggatt gatcattgac tcacctcatt
    19201 tttggtgatt agttgagtta aacgatcttc aaaagttttt gtagcatcaa agcccatttc
    19261 tttagcccga tgattcatcg cagcggcttc aataaccact gaaacatttc gacctgtttt
    19321 taccggaata cgaatacgag ggattctcac tcctgagaaa gttatctcct cattaccgtt
    19381 tccgagacga tcaaaaactt taccagcctc aaagttttca aggtaaatgg ccaattgaac
    19441 ttgcgatgag tccttcacag cactagctcc ataaagcgac atcacatcaa taatacctac
    19501 cccacgaatt tcaagcaagt ggcgcaagat ctctgctggc tctccccaaa gggtttcttc
    19561 atctttggca tagacatcca cacggtcatc cgcaaccaaa cgatgccctc gcttaactaa
    19621 ttctaaacca gtctcacttt taccaattcc agaatcccct tggataagaa cccccatacc
    19681 ataaatatcc attaaaacgc cgtgaacact agtacgttca gctaaagaag cgtcaagaaa
    19741 ataagacatt tctccagcta agcggctagt tgaaacacgg ctacttaaaa gtgaaatacc
    19801 ttcttctttt gcagcttgaa ccatttcttt tggaatggct aaatttctag agacaaccac
    19861 tgcaggagtg tcctttttga acatctcttt taaaacagaa taacggttgt gagaagtcat
    19921 ctgtgtcaga taagaccact ctttcattcc aaatagctgc aacctttctg gagcataata
    19981 atcaaaataa ccagtcattt caagacctgg acgtgaaata tctgatgttg ttatctcttt
    20041 tgacagcaag ttatctgtag cataaaccac attaagttta actttttgca ctagcatctt
    20101 tacagtaacg gtcataattt ctcctttttc aatattataa cacaaatcga ccgttagacc
    20161 cttgcaaata ctcatttttt cactactaaa tgagaacaac gacaccaaaa atggtcttag
    20221 ctctattaaa aactaggttt tatcatttag agatatgagt caaaaataag acaatggcaa
    20281 acaaatactt tactagtatc attgagcaat tagtgcttat tatggtgttt aaaaaggtat
    20341 catcttacta agtcaaaaag caaaaagcct tatcatagct gataagaccc ttcgcttact
    20401 tacatatcaa aacattttat tatcatcagt taatcacatg gttgctctta cgtgatgaat
    20461 tatcatttta actgattgtt taccagaacc agccatcttc ttcttcattt aagacatcag
    20521 cgtctttacg acgacgagga cctcggccat aacgttcttc aagcaagtct tccttgtaag
    20581 gtaagaagat ggccaacaaa atataaagca aaactccaaa accagttccg taaataagaa
    20641 gagccgctaa gacacgcgct agagcaagat cccaaccata cttatctgct aaaccagcaa
    20701 tgacacctgc aaccaagcga ttttttcgct gtttatagaa tttagtttcc acgtttcttt
    20761 acctcctcat tctatgatac tagtttacat tttttttcgc aaaataaaac cagtcttacg
    20821 actgattttt ttccataagt ttcccgtggc aatttccaca aacatattta gccagattga
    20881 tacgcctttt tcgttgataa acctgcccac aggtttggca actataatga tgattagtct
    20941 ttgatttaga gctagttgag acataacgaa gcccgtttac ttgagctagc agatctttga
    21001 aatccctatc tttatgatgg taccctcttc cttcaaaata aagatggtaa tggcagagct
    21061 catggcgaac aattttccga aaaataagct caccgtgctc ctcaagcatc ctagggttaa
    21121 aatccaaatg accatctttt ggaaaaaagc gaccgccagt tgtttttagg cgcttgttcc
    21181 aataagcctt gtggtgaaat ggtttgccaa aatcagccag ggaaacttct tggacataat
    21241 tagtgagtgt cacgcggtgg caataggctg agattgacct tgtggcggtc caagtcaatc
    21301 ttagagaccc aaacagttac caagtcaccc actgaaacaa cttgactagg gtggttgaca
    21361 aaggttttgc tcatttcaga aatgtgaata agcccatctt catgaacacc aatgtctaca
    21421 aaagcaccaa agtcaacgac gtttctcaca gttccttcaa gcttctggcc aatttccaaa
    21481 tctttcaaat caaggatatc ttgacgtaag attggtgctt caaagtcatc acgaaggtca
    21541 cgacccggtt taaggagatc agcaatgata tctttaaggg tttcctgccc aatagccagt
    21601 gtttctgcca tttggggaac ttgaactgct gctaaagttg ccttggcagc gtcgtccaag
    21661 tcctgaatac caagtacctt aaagagttct ttgactgctg ggtaggactc ggggtgaact
    21721 cctgtgttgt ctaaaatatt tttggctcct ggaattctga gaaaacctgc tgcttgctca
    21781 aacgccttag ctcctaatcg aggcactttt ttgatgtcag cgcgtgatgt cagtgctcca
    21841 ttttcttcgc ggtatttgac aatattttct gaaatcgttt tgttcaaacc tgacacatgt
    21901 gctaataaag atggactagc cgtgttcaca ttaacaccga cttggttaac cacagtatcc
    21961 acaacaaaac caagattctc actcaacttt ttctgactca catcgtgctg gtactgaccg
    22021 acgccaatag atttaggatc aattttaacc aattctgcga gtgggtcttg caagcgacga
    22081 gcaatagaaa tagcagagcg cttttccact gtgaggtctg gaaactcatg acgggctaac
    22141 tctgacgctg aataaaccga agcaccgctt tcgttaacga tgacatagga cgtatttggg
    22201 aaatctttca aaacgtctgc tacaaaggct tcactttccc gactggctgt tccgtttcca
    22261 atggcaataa tatcaatctg gtaagtctca atgagctgag tcagtgtttc tttagctgct
    22321 tgaatcttcg tttggctagc tggtgctacg ggataaataa cctgagtagt caacaatttt
    22381 ccagtctgat caacgatagc caattttgca cctgttcgaa aggcagggtc aaacccaaga
    22441 accattttcc cttttaaagg agacactaac aaaagatgac ggagattttc agaaaagagg
    22501 tggatagccc catcttctgc ggcatcactg agttctgaac gaacccgtct ttccatagct
    22561 ggaacaattt tctttttgat ggtctgattg atgacctctt caatataagg gttggtttct
    22621 ttgaaccgca cactgaaaaa gcgttgcatt ttctccaagt tatgctcaaa agacactttt
    22681 aagatgccta acttttcgcc acggttaagg gctaaagtac gatatccttg catgttagac
    22741 acttggtcag aaaaatcgta atagatttgg aaaacctttt tctcatccaa ctgctcatct
    22801 ttaagcgttg atactaagcg gctgtactgc caaatctcat tgtaggtcca agagcgtaat
    22861 ttagcatctt ctgacatggc ctccacaagg atgtccacag ctcctgctag agcttcttgt
    22921 gggctggcaa atccttctgt gacaaagggt tctgctgctg tttcaaggtt ttgcgcattt
    22981 tgtaaaatca aacgtgctaa tgggaacaag cctgcttcac gcgcaatcgt cgcttttgta
    23041 cggcgttttt ccttataagg caggtacaac tcttccaaat cagcgagttt ttcggttgct
    23101 tcaatactcg ttcgtaactg atctgtcagt tttccctgtt cctcaatttt agcgagaatg
    23161 gttgctttgc gctcattcag ggtagtgaga gacttatcca tatcaataat ggacttaatc
    23221 accacttcat ctagatttcc tgttacttct ttacggtaac gtgcaataaa gggaatggta
    23281 ttgccttgtg ctgttagggc aagcacctgt tcaatttgat ggagactaac tgacaaagcc
    23341 tctgcaatgt tgtgattatt gttattttcc ataatattgc tattataaca caaactatgt
    23401 taatgcaaaa aatgatagcg aaacattgtc ctaatataaa gttgcgtaaa gaatcaaaaa
    23461 aagctacttt tagtaaataa ctgacgaaac cctaaataac aatacctaat tttaaaaaag
    23521 aaagaagtaa acagcacttc cgctttggca ttgttctcaa atgatagtat aatagactta
    23581 tcttgaatga aagaaggtca tagatatggc aattacccat aaaaaaaatg atgaacttga
    23641 aaaaatgcta gcaggctttg catcaatccc tagttttgat aagcctttgg aagtcaatac
    23701 tgatggtaaa ctagcgacca aagaaaaaac gacagaacaa acaaaaccaa gcaaaccata
    23761 gctagtttgt ttcattctct taacatatcg tccattcgtt taaactgagt tgagatattt
    23821 ttagcgatca ggctaacaaa acgtatcagt cgaggctgaa cattcgtgtt tcagcctcct
    23881 cttttatcat ctagaaagga tatggtgaat gtcactcttt tccaaccttc ctcccagtgt
    23941 cttgcaatgg tttgcgattt ttatgtccat tatcattgaa gctcttcctt ttgtgctcct
    24001 aggaactatt ttatctggtt gtattgaagt ctttgtaacg ccagaattgg ttcaaaaata
    24061 cctacctaag caaaaatgct tgcgcattct gtttggaacc tttgttggat ttgtttttcc
    24121 ttcttgtgaa tgcgggatta ttcccatcat caaccgcttt ttggaaaaaa aggtgcctag
    24181 ttatacggcc gtcccttttc tggcaacagc gcctattatt aatcctattg ttttgttcgc
    24241 tacttattct gcttttggga attctttgcg ctttctgata cttcgcttgg tcggtgctgc
    24301 actagtagcc attacacttg gggtaatgtt agcttttatt gtagatgata acattctcaa
    24361 ggataatgct caacccgtgc attttcatga ttattctcat gaaagcttgc caaaacgcat
    24421 ttatctagca ctggtacatg ctattgatga attcttcgac accggtaggt atctcgtatt
    24481 tggaaccttg attgcctctg ccatgcaaat atatgtccca actcgtgtgt tgacaacgat
    24541 tgggcacaat cccttgacag ccattttgat catgatgctg atggctttca ttctatcatt
    24601 atgtagtgaa gccgatgcct ttatcggagc ttccttatta tccacctttg gagtggctcc
    24661 agttcttgcc tttcttttaa ttggtcctat ggtggatatt aaaaacctta tgatgatggt
    24721 aaaggctttt aaaggacgct tcattgtcca atttatcggc gtatccgttc ttatgattgc
    24781 cgtttattgt ttacttgtgg gggtgctcta atgattcgct ttttaatttt agctggctat
    24841 tttgaactaa ccatgtatct ccagctgtct ggcaaactgg atcagtacat caatgttcgg
    24901 tattcttacc ttgcttatat ttccatgatt ttatctttca ttttagctct tgttcaactc
    24961 tatacctgga tgaaaaacat taaggtacat agccatttaa ccggaaaaat cgcaaggctg
    25021 accagtcctt ttatcttggt ctttcctgtt ttgattggtc tccttgtgcc gaccgtgacc
    25081 ttagattcca ccacggtttc tgctaaaggc tatactttcc cattggctgc aggagcctct
    25141 aaaacgggag tcagtgatga cggtaccacc attcaatacc tcaaacctga taccagcctt
    25201 tactttacaa agtctgccta ccaaaaggag atgcgtcaag aactccataa atataaaggc
    25261 aagaaaccgg tcaccattac cactgaaaat tatatggaag tgatggaatt gatctatctc
    25321 tacccagatg agtttcttga tcgtgacatc caatacactg gctttgttta taacgaaccg
    25381 ggccatgaca actaccaatt ccttttccgc ttcggcatta ttcactgtat tgccgactca
    25441 ggagtgtacg gacttttgac cacaggtaat caaaccagct accctaacaa cacatggcta
    25501 acagttaaag gtcgccttca tatggaatat gataaaaact tagaacaaca tctacctgtc
    25561 cttcaacttg ctgaggttca tcaaaccaaa gaacctaata atccttatgt ttaccgcgtt
    25621 ttttaaagac taataaaaaa gcacttgttt tttatcaaaa caaatgcttt ttgagtttta
    25681 tcagttagca taggaacaga gtcaccatct taaacaagac ctatttagga aaaattatcc
    25741 tcaaggtaac gtccattggt ttgaatgact tgtcggtacc aatcaaatga ttttttctta
    25801 tagcgtgtga gttgaccagt accatcatca tttcggtcaa cataaataaa accgtagcgc
    25861 ttacttaatt gggctgttga catagagaca caatcaatac agccccaagc agtgtaaccc
    25921 atgacctcaa cgccatcttc aatcgctttg gcaacttgta cgagatgctg actcatgtag
    25981 tcaatgcgat aatcatcgtg aaccgtcatg ctgccatctg cggtttgcac cagctgatcc
    26041 tttgcaccta agccattttc aacaataaac aaaggaatct ggtagcggtc atagtactga
    26101 ttcaagacca atcgcagacc aattgggtct atctgccaac cccactctga agattctaaa
    26161 tatggattgg acaaaccacc tagaatattt ccctgaccag atgtgtaagc ttcaggattg
    26221 tgggcttgtg tgacactcat gtaatagcta aaggatagaa aatcaaccgt atgttccgct
    26281 aataattcct tgtctccgtc ttcaaaaaca atctcaatcc cattttcttt aaaatacgac
    26341 ttaatatatg aaggatactt gcctctcaca tgaatatctg aaaataagag attaagattt
    26401 tcaaatgcat gcgcagcaag tatatcacga ggatcagagg tcattgggta agcaggcatc
    26461 gccaaaatca tacacccaac tttaacatca ggattgattt catgcgctag ctttgtgaca
    26521 ctagcagatg ctaccaattc atgatgaata gcttggtata aatcttgcag gctcagcttt
    26581 tctttttctg tcatgatgcc accactggta aagggcatgt gtaaaatcga gttgacttcg
    26641 ttgaaggtca accagtattt gactttgtcc ttatagcgtt ccatgactgt ctgagcaaag
    26701 cgctcaaaaa agccaatcag acgtctatct gtccaaccat tataggcttt ggccaaatgc
    26761 agaggtgtct cataatgcga caaggtcact aagggttcga taccatagtt caacaattca
    26821 tcgaataagt catcataaaa ttgcaaacct gcttcattgg gctggtcatc gtcaccattt
    26881 ggaaaaatac gagaccaagc aatagacgtt cgaaagactt taaagcccat ctcggcaaaa
    26941 agagcaatgt cttctttgta gcggtggtaa aaatcaatcg cctctaaagt taaattatcg
    27001 ctggtaggac tatctgtcca ttctcctagc ccaccatttg gtagaacatc ttgtacggac
    27061 aaaccttttg cgtctgcctc aaatgctcct tccacttggt tggcagctac tgcgccgccc
    27121 cataagaaat cttttgaaaa aattcccatt ctccctctcc ttttaggtta taaaatgact
    27181 aacaaagcgt cctctttagt caccattttt ttgtcatctt gaataataat ctccaactgc
    27241 tcagccgaat tggtcacgac cacaggactg atgagtgaat agccttttga agtaatgaaa
    27301 tcagtatcca tttttagaag tgcctggcct cgtttcacga catcgccaac agacactaac
    27361 tgctcgaaac cttgcccttt taactcaacc gtatcaatac cgacatgaat caagacttcc
    27421 acaccacgtg tgttttttaa ggcaacagca tgcttggttg gaaagaaagt gatgacttca
    27481 ccatcaaagg gggcatagac cacaccttcg cttggtaata tggcaaatcc ttctcctaat
    27541 aacttggaag aaaaggtttc atcaggaact tctgatagaa aaagcatctc acctgtcatt
    27601 ggagcataca acgtttgttt ggttgacaag ccgctcttaa cttgtgacat atctgctgca
    27661 acactcactt gctctgggga ttcttcatca attcccatga accaagtcaa actgaaggct
    27721 aacacaatcg tcgcagctcc ggcaatcaaa gcatttgtaa aattagctcc gcctgatggg
    27781 ttgatgtact gtggtaaacc aataatagaa ggtacgacaa aggtataaga agcaatattg
    27841 acaaaaccaa taaaggctcc gaccagacca cctgaaatca tggctgcata gagtggcttt
    27901 ttaaatttca gtgtgacccc ataaagtgcc ggttccgtga tacctgccag caaagctgaa
    27961 atccccgcgg caagggcaac ttgacgtgtt tgtttctgct ttgttttaaa ggcaacagcc
    28021 aaagatgcgg ctccttgagc caaattagaa gccaacattg ctggtaaaat caagacatct
    28081 ggtgttgcga ccgaagcggc caaaaagatt ggtgcaaaag cccagtgcat ccccgtcatg
    28141 acaacaagcg gcataatcgc tcccaaaatg gcaagtgcta gccacggagc cacatgataa
    28201 atagcgagca tggtattaga taacccttga ccaataatca caccaagagg acctacaacc
    28261 actaaagcca aaaagccaga aatcaaaatg attaaggttg gctgtaagaa gcttttcatc
    28321 acgcttggca ccaagcgatt gacccatttt tcaatgtatt gcatcaagta aaccatcatc
    28381 aaaatgggaa taacagatga gccatagcta gcaggagtga ctggtgctcc aaataaagcc
    28441 aatggtttac cttctgctac cattgcaaca aaggcaggat gcaataaaat ccctgcgatc
    28501 gtcgctgcta aaacaggtgt caccttaaag cgcgccgctg ctgaataggc aatcatgaca
    28561 ggcataaaat aataagccgc atcgccaaag aagttaataa aggcaacggt ttgagagtcc
    28621 gcactcgcta agccaagcat agtaagcaag attcccacca ctttgagcat tccaccacca
    28681 agaagggctg gaatcagcgg tgtaatcacg ccagcaatgg tttcaatcaa gcgctcaagg
    28741 atacttcctt tcgcttttga agaatgatct tcatcttgat tgtcaaaatg ccctaggctt
    28801 aaaaaggctt ggtaataatt attgacatca ttgcccaaga taatttggta ctggccgttt
    28861 ttgcgcatca cgccaatcac atttgaaatg gctttgactt gctgatcttt aactttctca
    28921 tcatttttca gtactaagcg tagacgtgtt acacagtgtg tcacacgttg aatatttgtt
    28981 ttgccaccaa ccgccgctaa aatagccttg gcagtttctt gatacgtcat attaagtctc
    29041 ctttaaatgt ttcactaaac gtttaacatg aaccgtcaaa tacaaaagtt cgcttgaatt
    29101 aaggtggtag ttgtaccgct tttttatctc ctcactaata tctaagacgc accggtattc
    29161 tttagggtat ttcgttttca ccactaaaag cagttccgca tcatcatcat cgtaagacat
    29221 ctcggataac acacgttgag caaataattt aatatgggtc ataaaacggt aataatcgat
    29281 ggatgtgtca tccaattctg ttttgaagtc tgttttgatt ttttgctcaa tataagaaac
    29341 aatctccgtc aggcgatgtg gctccttaaa aggagtgtcc aaactagcat tgacaaaatg
    29401 catggcaata aaagctgctt catcaatagc tagagtgact cctagattac gctcaattag
    29461 ctctaaggct tttaccccta gactatattc atcaggataa tagcgttgaa tttcccaccg
    29521 tagtgggtta tgaatcaaca ttccttgctc atgcctttca atagcagagt gaatatgatc
    29581 cgttaaatta atatagagaa tttcatctaa ggttttgcct aatttaattt tacctagatt
    29641 gataattctc tctgagcaag caacaacttc ttgtggcacc gtaataaata actctgtgaa
    29701 gcgattcata ttgtcactat tttttaaaac aaatgatgtt tcgatagcat taagctcaat
    29761 actatctcca actttttttc caaaggcaat cccttttccc atcaataaaa tatctaatcc
    29821 ttgatggttg gtcgaaatcg ccgcattatg gtttaaaacg cgtttaatca acataaaagt
    29881 cctagtcctt tagtatcaca ataaaaaatg acctatccaa aagctcgcta aaaagcactt
    29941 ttcgaatagg tccctgctca caaaactgtg ataacatcct ttaatattct gattgttatg
    30001 tctctagcct gcttacctga agtaatcaca tctgagaacg ctttaatgtt atcatctctt
    30061 taagattctg tcaagcgttt tcaaaaaatt ctctgaaaga gcaactattg ttattcgtat
    30121 ctattgattt agcaaaaaag agtttctgac aattttaaga ttatatgata caataacaat
    30181 cgattagata aaggagttat catgtcacat catcaacaaa ccgtttcaaa acaaacaatt
    30241 atggcgatta tcgccatagc actcattggt ttttcaggaa ttttgtctga aaccagtatg
    30301 aatgtcacct tcccgacact gatgtcagtc tatcagttgc ccttaaatag cttgcaatgg
    30361 atgacgacca tttatttact agcagtggcg attatgatga ccacttcggc tacactgaaa
    30421 aaaaatgtgc gggaaagacc cctctttttt atggccacag gtctttttac atttggcacc
    30481 atcttggccg ttctgaccca gtcctttgcg atcatgttgc tagcccgcat ttttcaaggc
    30541 attggtactg gtctggtaat gcctcagatg tttaatatta ttttagagcg tgtcccaatg
    30601 cataaggtag gtctatttat gggatttgct ggtcttatta ttagcttagc acctgctttt
    30661 gggcctactt atgggggctt tatgattagc cattttagtt ggcaatggat ttttatctgt
    30721 atactccctg taccactgat tgcaggtatt ctagcttatt attacctcga agattctcca
    30781 gtaagcgaaa aagtaccctt tgactggttg gcatttattg cactatcgat tagcttaact
    30841 tctgccttat tagctattac tagtctagaa aacggaagtg ttaatttgta ttacttaggg
    30901 ctttttattc tcagctttgt cctctttctc tacaagaatc tcacagctaa gcaacccttt
    30961 cttgatattc gcattctcaa aattccctct ctaacctttg gcctgattcc cttttttgtc
    31021 ttccagctga ttaatttagg cataaatttt ctaacgccaa actttattgt catggaaaag
    31081 attgctaata gttctcaagc tggtatggtg ttactacctg gtaccttact cggagctcta
    31141 ctagcacctg cttttggtaa actttatgat caaaaaggag caagactttc gctttattta
    31201 ggaaatgcct tatttagttt atcattgatt attatgacac ttcaaacaag acattttatg
    31261 cttttaccat tcactctttt atatatttta ttcacgtttg ggcgtaacat gggctttaat
    31321 aatagcttag ccacggctat tcgagaattg cctgccgaga aaaatgccga tgccacggcc
    31381 atttttcaga tgatgcagca atttgctggc gctctaggaa ctgctatggc atcactgata
    31441 gcaaatagtc agccagaatt cacaagcggt gtccagtctg tctaccttct ctttactatt
    31501 tttgctctac ttgattttat ctttttcttt gctatgtttt accatttagg gaaaaaagga
    31561 ttagcctaag ctgacttact taaaaaccag cctcgttctg ttgaacaaga ctggtttttt
    31621 tcttataaaa taccttctaa aagacctttc ataaagtctt ctgagtggaa ttctccaata
    31681 tcatccactt tttcaccaaa tccgatgaac ttgactggaa tgtctaattc ttgacgaatg
    31741 gctaggacca ccccaccttt tgcagtccca tcaattttcg ttaaaattaa gcctgttaaa
    31801 ggtgttattt ttgaaaattc cttggcttgg ctaagcgcat tttgacctgt tgacgcatct
    31861 aaggccaaaa gcgtttcatg aggggcatct ggtagaacgc gcttaatgat acgacccatt
    31921 ttttccagct ctgccatgag attttctttg ttctgcaggc gaccagccgt atcaatcaga
    31981 agaatatcta cttcttttgc cacagctttt tccatcccgt caaaaacaac agacgctgga
    32041 tcagcctttt cagggccagt aatgactggc acatctactc ggcgtcccca ttcgactagc
    32101 tgagccacag cccctgctcg gaaggtatct gctgccacca acatgacttt tttaccttct
    32161 tgtttgtaac ggtaagccag cttcccgatt gaagtcgttt taccaacccc gttaacacct
    32221 acaaaaagca taaccgtcaa gccatcttga taattaatgg cttcattata acgcccgtct
    32281 ttttcataga tatctaccaa tttttccaca atcacacgtt tcaaggcatc tggttttttc
    32341 gcattttcca gtttggcttc ataacgcaac tcctctgtca aagttgtcgc cacatgtaca
    32401 ccgacatctg ataagatcag catttcttcc aagtcttcaa agaattcttc atccactcgc
    32461 ctgaaattgg caaagaaact gttcagacga gctgagaagc cagtccgcgt ttttttcaag
    32521 ctacgcttgt atttttcttg atctgtctca ggaatggtat cgtaggttgc cttgttttga
    32581 gaagcttctg ctgaggtaga ggcctgattt tcttgtggaa cctcttctgg catcactgga
    32641 accgttgccg ctgctttttc ttgaaggctt ttttcaagag cagccttacg acgatagtag
    32701 tcttccataa cctgttggct actggattgc tcttttgaag tgtctactgc ttcttcattc
    32761 gccgaatcat ttgaggcatc agcttgggct tgttttgttg gaaactgttc cacaactggc
    32821 tggtcaacaa ggtcttttgg ttctgatgcc gtagattgac taagttcact taccttaagg
    32881 tctgttttgt cggcagcaaa cttattggta tcttctgatg gatgattact attgtcacct
    32941 gtttcttcct caacctgtgc gtttactgaa gctgcttcgg gaccattttc caccgaacct
    33001 gcgccctctg aagaaacgtc ctcttgttga accgcctcaa tcttgtcttg ctcattggct
    33061 ttttcagaaa gctcctcttt ctgagttgtt tctgtcaaaa gattttcttc taacttttcc
    33121 tcagctacct tcggggtttc ttttttccca aataaacgat caaataatcc cattaactct
    33181 cctcttctaa aataaatgtc ttcacagctt ctgcaactcc agactcatca ttggtcaagg
    33241 tggtcacggc atcggcaacg gctttagctc ctgaaacacc attagccata gctactccaa
    33301 gtcctgccca ttctaacatc gcaaagtcgt tagcttcgtc tcccatagcc atgacatgtc
    33361 tagcgtcaag gccaaggtgc tggcaaagca agtttaatcc aaaagcttta tcaatgcctt
    33421 ttggcatgat ttcaaaaata atatctcgtg atttaaaggc ttcaaaatcc tcaaataagc
    33481 ttggggatag ctttataatc tgctgatcca aaaagtctgg atcggtgact gtcacaattt
    33541 tgttatagat aatgtctttt ggtagttccg ctacagatgt cacctcaata aaggtcaaca
    33601 gtgggttagc tagatggtac tgcgaatgcc taccgtcctt acttggaatg ctatacacat
    33661 ccccaccact gataatatct gttggtaagc caaccgcctc taaagcttgc tgaatctggc
    33721 aaacctggtc aaaactgaga gagctcttat ccaaaacctc ccctgtattt cgctgaacca
    33781 aacccccatt aaaggtaata ctgtagtcat cgtggtcaag caagtccaac tcttctagca
    33841 agttaccgat ggcctttaga gggcgacctg tggtaataac caccttaacc cctttttctc
    33901 taaccgctgc taaggctttt ttattggcat ctgtgacaat tttttcagtg ttgtagaggg
    33961 taccgtctaa atcaagcgcc aaaattctaa tattcatgag ttacactagt ccttttaaat
    34021 acgttagtac tgctccgtca ttacagtggc caatgacttg gtctgaaatc actttgattt
    34081 caggtcttgc attttccgta gcaatagccc taccagcaaa ctccaacatc tggtaatcat
    34141 taaaattatc cccaaaagca atcgtttctg ctttcttaat gcccaaggct tgacaaagat
    34201 gctccatacc aaagccctta ttcacctctt ttaagataat atcaatggaa tcaaagccgg
    34261 tggtcactgc agtggcataa gggagggctt gattcaacca atcgcttcct tctaagacgg
    34321 tatggcctgt gaagttggta gaaactttaa aaatggcgtc attctccata tcttcaaaac
    34381 cattaataac cttaacatta gcatagtaat gttttgtttt ttgaatgtac tcttctgatg
    34441 cgccttttaa aatataggct gccttttgcc cagaaaaaac catacctgtt tctacatagt
    34501 gaggattggc taaaatcttt tttgcaactt cggtatactg ctctttggtc atcatatctg
    34561 caaatagaat ctcaccacgg tattgaacca cggaaccatt ttctgcaata acagcaatct
    34621 gatccaaaaa gggctcaaag agctgatcaa tggctaagag cgagcggcca ctcgaaaccg
    34681 caaataaaat gcctttttcg gctaactttg gcaagagcgc agctagttgc tcttgattat
    34741 aagtaccatc ttcagctagg aaggtaccat ccatatctgt tgctattagt tttatcatac
    34801 aatgtctcct ttttttactc taggggggtg caaacgccat ttagggtgtg cactttttaa
    34861 cgatttaaat aagccccatc atttctgcat tctgttcaat ttttcttatt atatcataaa
    34921 aagcagcttt cattccattc gtaaaaaaca gccagattat atttcgataa cctgaccgtt
    34981 tttgaagtga actgctactt tttttatttt tataaatgac cactggatgt atactataaa
    35041 agtagacaaa actttgtgtg ttacaatctg ttttaaaaga cacaaaaacg aaaggacaac
    35101 ctatgtcaac agttaaacgc tacagatgaa gaatttaaac aatcccttgt caacctttat
    35161 caaactggaa aaactcagtc tgaactctgt aaagactatg gggtatccgc ttctgcgctt
    35221 gcaaaatgga tcaaacagta ttctcaagtc aaactcgaag ataattctgt acttactgcc
    35281 aagcaaatac aagagctaca aaaacgtaat gcgcagcttg gagaggagaa ccttatccta
    35341 aaaaaagcaa gtgccatatt catgcaaaac ttaaagtaag actcctcgct gtctatcggt
    35401 tacgctttga acacgccaca acaaccttgt atcgtgtttt acgtgtcaat cgctccactt
    35461 actataaatt tctaaaacat aagccctcaa aaagagaatt ggataagcaa atttatagaa
    35521 aacaaatact tgagatttat accaaagcga acaaaagact tggtgtgaag tctatcaagg
    35581 tcattcttca aagagactac gacacaaaaa tctctgaagg aagaatttac cgtctaatga
    35641 agaatatggc gctccctaaa atggctaccg ttaagccaaa aacagctctt aaaaggactc
    35701 aaaaaacgta tcctcaaaac ttactcaacc agaaatttaa tcctgacaaa ccaaatcaag
    35761 tatggtctac tgacttcacc tatatttcta ttggatataa gaaatatgtc tatctctgcg
    35821 caatacttga tctctattct agaaaatgta ttgcttggaa attgagtcat catatggatg
    35881 caaagttagc atgtgacact ctagaattag ctcttaataa aagaaagatt gaaggaacac
    35941 ttctctttca ttccgaccaa gggtcacaat ttaaggccag gaaattgaga aaaataattg
    36001 atgacaacac tatcatgcat tctttttcta aacctggata tccttatgat aatgccgtaa
    36061 ctgaagcatt tttcaagtat ttaaagcata gacaaatcaa ccgaaaaaag tatcaaaata
    36121 tcaaacaggt tcaattagac tgctttgaat acattgagaa tttttataac aattacaacc
    36181 cacatacggc taatctagga ctaaccccta atcagaaaga agaaaattag tttaacgtaa
    36241 taaaataaca cggttttctg tctacttatt tgacatta
//
LOCUS       DAUCLR010000009        34858 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.009, whole genome shotgun sequence.
ACCESSION   DAUCLR010000009 DAUCLR010000000
VERSION     DAUCLR010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34858
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.009"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..889)
                     /locus_tag="VGT87_000333"
     CDS             complement(<1..889)
                     /locus_tag="VGT87_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813222.1"
                     /translation="MIRIKNITKSKFFGTAIILLQQLIALLILVYNRENLSLLFSEKV
                     ALVMTLIDTAFIWLATILRQKQGDIFKRIISIISLTIWQYLVSVLTRGTPLFLSSGLQ
                     IILLYCYTVEITNLILYGHKQFKDKLDKGLLIIIFVSITSLFVNSILFNFLFLMVFTI
                     LHLYPLLVIVLYYRSFRQQISVVRRSLILFSLLLLVILGSELYGEMLDVNQAFNNLGW
                     YLFPLIMSVIYYFKTIHDKLSFVTQRWLGDYKARIELLFLLLILCWIVLMKVLVKDFL
                     LFFIVVDASTLFCLVVISCI"
     gene            complement(929..2845)
                     /locus_tag="VGT87_000334"
     CDS             complement(929..2845)
                     /locus_tag="VGT87_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEQ3813223.1"
                     /translation="MIWSITKSNIKKNFSLYRIYFLATIGLLSIFIAFLNFISDKIIT
                     EKIGDSGQALVIANGSLIFLIVFLVVFLIYFNNFFVKKRSQELGVLAILGFSKRELTK
                     LLTLENLVILVLSYLVSLLLGPTLYFLAVLAITHLLELTLEVQWFITANEIIESLGIL
                     VVVFLINVITNGLIISKQSLIEFVNFSRKAEKKIKIRKVRAITAITAITALLLSYILC
                     LATVFSSTRNMLLSIGMVPVSLLIIVLVVLGTVFTIRYGLAFVVSLLKENKKRLYRPL
                     SNIIYPKFNYRIATKNKLLTVLGGLLTVTVSVAGMMVMLYAYSLNGIERLTPSAIEYN
                     VESENGQVNVTTILENDQVSLVDVDLLRLNTNPEVTITESGQTIPYFDIINYSDYKEL
                     MKAQGRINSIEGSKSLPLLINYYPTEISLGKTFNLGNAYDVTVKQVSTHNVFSFSTSV
                     TTLVVSDKLYAKLSSCFPEKEMTIRTFNGNSIRSSEAFYNQFSMVPDVISSYSKEYTV
                     KTANIATYIFITFLSILFIICTGSILYFTSLIEIMENKEEYGYLSKLGYSKKMIHRIL
                     RYETGILFLIPVFIGIVNGGMLLIYYKYLFMDTLVAGNIIMLSLLLCLLFFLIIYGTF
                     YVLTLRSVTSIIKN"
     gene            complement(2847..3584)
                     /locus_tag="VGT87_000335"
     CDS             complement(2847..3584)
                     /locus_tag="VGT87_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ3813224.1"
                     /translation="MNQLLLKNVCKRYPNQPNYALDHINLTVRKGEFVAVMGRSGSGK
                     TTLLNVTSIIDKIDSGSIYCADKEISVFSDNEATSFRKNDIGFVFQDYMLLDSLTIRE
                     NISVALSLKNIDSSKIDDLINSYAKRFNLYEQLKKYPYQLSGGQRQRVSIIRAIIKEP
                     EIIFADEPTGALDLKSSEETMMILSEINKTEKVTILMVTHDVLSASYADRVVLLKDGK
                     LHMEIDKKDCGESFYDVISQALSDRGE"
     gene            complement(3581..4114)
                     /locus_tag="VGT87_000336"
     CDS             complement(3581..4114)
                     /locus_tag="VGT87_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ3813225.1"
                     /translation="MDKKDFRDISAFVSQDSPIFDGDVMYNISLGRESVSGEQVIETC
                     KRVSIYDDIISMPMKFHTPLFRDNPSLSGGQKQRISLARELVTTPRILVLDEPTSALD
                     VKTERIIQKNVEALHCTRVLVTHRLNTVEKADKILIMDNGKIIDYGSHHCLYKNNEYY
                     RDLYDSYMNNYQEEGIK"
     gene            complement(4116..5741)
                     /locus_tag="VGT87_000337"
     CDS             complement(4116..5741)
                     /locus_tag="VGT87_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4135 domain-containing protein"
                     /protein_id="HEQ3813226.1"
                     /translation="MIKRDELKLEYQNHQFYEYVNSIFDYDILDTSIRETSEVLRKKT
                     HKLFYSEFENQLFETIMFLSMKTLVLDINHFSKEIENKSEAYEQYIQQIREENGINHF
                     FDRYPYLLKQINKEVGLIEESYSLLFDRFLEDLSEIRSCFNISEPLSNVAFSLGDSHS
                     KKQTVVKIAFKEKSVYYKPKSYHSHSILLELTSLLKSSNIPSFSLPKSLVKADYCWQL
                     GVAYTSSNKDEVAKIYFKYGVLAAFSEIFSITDLHMENVIVSGGDLYLIDVETFFQRK
                     LNVQNQNFEGITVDTYQRIYETSLSNGLFPVQFEKNSAPNVSGISGKGGKRKKGKYEL
                     INKNRGDMKLVKTDYFQEDGYNIPTLNGKVVEPLDYANEVIADFRECYTFLMSQRAKV
                     KKILEDFPKLKTRAIFRNTSDYGKFLQASTNPKYLFSEKKRENLFSILHESKHIEQFI
                     VVSEIKDLMNGDIPYFSMDTSGNVYNSLGTVIGNLGETTSLFDNIATLNDERMKFTCE
                     LLGIVLKKPIKHWEREKGKSYQFLSISSEHYLL"
     gene            complement(5825..5980)
                     /locus_tag="VGT87_000338"
     CDS             complement(5825..5980)
                     /locus_tag="VGT87_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A2 lantipeptide"
                     /protein_id="HEQ3813227.1"
                     /translation="MSFMENSKDILTNAIEEVSEKELMEVAGGKKGSGWFATITDDCP
                     NSVFVCC"
     gene            complement(6485..7891)
                     /gene="lacG"
                     /locus_tag="VGT87_000339"
     CDS             complement(6485..7891)
                     /gene="lacG"
                     /locus_tag="VGT87_000339"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEQ3813228.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLSEEFGVNGIRISIAWSRIFPTGKGEVNPKGVEYYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSDGDFLNRENIEHFVNYAEFCFKEFSEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLFKDSGYSGEIGVVHAL
                     PTKYPFDANNLDDVRAAELEDIIHNKFILDATYLGKYSDKTMEGVNHILEVNGGELDL
                     REEDFTALDAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     KAPVDVPKTDWDWIIFPQGLYDQIMRVKADYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVISDAISDGANVKGYFMWSLMDVFSWSNGYEKRYGLFYIDFETQERY
                     PKKSAYWYKKVAETQVIE"
     gene            complement(7964..9661)
                     /locus_tag="VGT87_000340"
     CDS             complement(7964..9661)
                     /locus_tag="VGT87_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEQ3813229.1"
                     /translation="MNGLIAQIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIENLLMTPYNYSMGILGLFVAGTTAKALTDSMNRSLSSTNQ
                     INFISTMLAAIVGFLLMAANPAKDGGFLTGFMGTKGLLTAFIAAFITVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTLSVVLLYVIELIVHSTLGVNVAESIGKLLAPLFQ
                     AADGYVGITIIFGAFALFWFVGIHGPSIVEPAIAAITYANAETNLQLLQAGEHADKIL
                     TSGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNKAIGRASVVPTFFGVNEPILFGAPL
                     VLNPIFFIPFILAPIVNVWIFKFFIDTLGMNSFTANLPWTTPAPLGLILGTNFQVLAF
                     ILAALLIVVDVIIYYPFLKVYDEQILAEEAAGTNSSDVLKEKVAANFDTKKADAILEK
                     SAAKETKEITDQTNVLVLCAGGGTSGLLANALNKAAKEYGAPVTAAAGSYGAHREILP
                     QYQLVILAPQVASNYEDMKVETDKLDIKLAKTEGAQYIKLTRDGQGALDFVKEQMEE"
     gene            complement(9661..9978)
                     /locus_tag="VGT87_000341"
     CDS             complement(9661..9978)
                     /locus_tag="VGT87_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEQ3813230.1"
                     /translation="MNREETTLLGFEIVAYAGDARSKFLEALKAAQEGDYAKAEELIA
                     AGSDCLNDAHNAQTSLLQKEAAGDDLAYSVTLMHGQDHLMTTILLQDLMKHMIELYKR
                     GAK"
     gene            complement(10002..10985)
                     /gene="lacD"
                     /locus_tag="VGT87_000342"
     CDS             complement(10002..10985)
                     /gene="lacD"
                     /locus_tag="VGT87_000342"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEQ3813231.1"
                     /translation="MTITLTENKRKSMEKLSIDGVISALAFDQRGALKRMMAQHQTKE
                     PTVEQIEELKSLVSEELTPFASSILLDPEYGLPASRVRSEEVGLLLAYEKTGYDATTT
                     SRLPDCLDVWSAKRIKEAGAEAVKFLLYYDIDGDQDINEQKKAYIERIGSECRAEDIP
                     FYLEILTYDEKISDNASPEFAKVKAHKVNEAMKVFSEERFGVDVLKVEVPVNMKFVES
                     FADGEVLFTKEEAAQAFRDQEASTDLPYIYLSAGVSAKLFQDTLVFAAESGAKFNGVL
                     CGRATWAGSVKVYIEEGPQAAREWLRTEGFKNIDELNKVLDKTASPWTEKM"
     gene            complement(10989..11918)
                     /locus_tag="VGT87_000343"
     CDS             complement(10989..11918)
                     /locus_tag="VGT87_000343"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEQ3813232.1"
                     /translation="MILTVTLNPAIDVSYPLDELKCDTVNRVVDVTKTPGGKGLNVSR
                     VLNDFGETVKATGCVGGESGDFIINHLPDSILSRFYKISGDTRTCIAILHEGNQTEIL
                     EKGPLLSVDEIDGFTHQFKYLLNDVDVVTMSGSLPAGMPDDYYQKLIKIANLNGKKTV
                     LDCSGNALEAVLKGDSKPTVIKPNLEELSQLLGKEMTKDFEALKEVLQDELFEGIEWI
                     IVSLGADGVFAKHKDTFYNVDIPKIKIVSAVGSGDSTVAGIASGLANDEDDRALLTKA
                     NVLGMLNAQEKTTGHVNMANYDKLYQSIKVKEV"
     gene            complement(11964..12479)
                     /gene="lacB"
                     /locus_tag="VGT87_000344"
     CDS             complement(11964..12479)
                     /gene="lacB"
                     /locus_tag="VGT87_000344"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEQ3813233.1"
                     /translation="MKIAVGCDHIVTYEKIAVVDYLKSQGHKVIDCGTYDNVRTHYPI
                     FGKKVGEAVASGEAELGVVICGTGVGITNAVNKVPGIRSALVRDMTSAIYSKEELNAN
                     VIGFGGKIIGGLLMNDIIDAFLAAEYKPTEENKKWIEKMDSLQHASQDQNNPHFFDEF
                     LEKWDRGEYHD"
     gene            complement(12514..12942)
                     /gene="lacA"
                     /locus_tag="VGT87_000345"
     CDS             complement(12514..12942)
                     /gene="lacA"
                     /locus_tag="VGT87_000345"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEQ3813234.1"
                     /translation="MAIIIGADKAGQELKEVIKDYLKEGKHEVVDVSENEVRDFVDTT
                     LAVAKEVNASEDNLGIVIDAYGVGSFMVATKIKGMVAAEVSDERSAYMTRGHNNARII
                     TLGSEISAPGIAKNIIKGFVEGKYDGGRHQVRVDMLNKMC"
     gene            13388..14161
                     /locus_tag="VGT87_000346"
     CDS             13388..14161
                     /locus_tag="VGT87_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004270277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEQ3813235.1"
                     /translation="MKKKERHEKILDILKVDGFIKVKDIIDEMNISDMTARRDLDTLA
                     DKGLLIRTHGGAQYLDYSSAKDEGHEKTHTEKKVLQTTEKKQIAQRAKNIIKDGETVF
                     IGPGTTLEHLAVELKNRNIRVITNSLPVFLILHPSTTIDLILIGGECREITGAFVGAI
                     AINNLESLTFSKAFVSANAVYNNSIATYSDLEGEVQRVALNNAVEKILLVDSTKFESY
                     DFYNFYQLDQVDLIVTDKNVTTDIIEKYKKFSKIIIADK"
     gene            14811..15098
                     /locus_tag="VGT87_000347"
     CDS             14811..15098
                     /locus_tag="VGT87_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-damage-inducible protein J"
                     /protein_id="HEQ3813236.1"
                     /translation="MTTVKKNRAVTFQANKELVSEAMTVLNKKNLTLSSALRLFLQNV
                     VVTNEVDLLTEEELEKEKLFKQFQAEINKNIEDVRQGKFYTSEEVRAELGL"
     gene            15088..15423
                     /locus_tag="VGT87_000348"
     CDS             15088..15423
                     /locus_tag="VGT87_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEQ3813237.1"
                     /translation="MDYKKYQIIYAPDVLEKLKEIRDYISQNYSSTSGQRKMEQIISD
                     IEKLEVFPEVGFEADEKYGSKISHYHSTKGYTLSKDYIVLYHIEGEENRVVIDYLLPT
                     QSDYIKLFK"
     gene            15575..16045
                     /locus_tag="VGT87_000349"
                     /pseudo
     CDS             15575..16045
                     /locus_tag="VGT87_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982723.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase family
                     protein"
     gene            16008..16508
                     /locus_tag="VGT87_000350"
     CDS             16008..16508
                     /locus_tag="VGT87_000350"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEQ3813238.1"
                     /translation="MAALRKGELCALKWADIDFQSETLSVNKSLGRLDGQAIEKSTKN
                     DFSVRKIKLDSETLSILQEWKAICQKEKAQLAVAPLSIESDFIFTYCTREGSIEPLHA
                     DYINNILSKVIKQHSLKKISPHGFRHTHATLMIEIGVDPVNTAKRLGHAISQMTLDTY
                     SHATAV"
     gene            complement(16676..17068)
                     /gene="rpsI"
                     /locus_tag="VGT87_000351"
     CDS             complement(16676..17068)
                     /gene="rpsI"
                     /locus_tag="VGT87_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEQ3813239.1"
                     /translation="MAQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLIINQPFAVTSTEGSYDVFVNVVGGGYGGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(17089..17535)
                     /gene="rplM"
                     /locus_tag="VGT87_000352"
     CDS             complement(17089..17535)
                     /gene="rplM"
                     /locus_tag="VGT87_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEQ3813240.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKVYYTHSMYPGGLKSITAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGGEHTHAAQQPEVLDISGLI"
     gene            complement(17753..17959)
                     /locus_tag="VGT87_000353"
     CDS             complement(17753..17959)
                     /locus_tag="VGT87_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ3813241.1"
                     /translation="MKNLKLKAARAGKDLSQQALADLVGVSRQTIAAVEKGDYNPTIN
                     LCIAICRVLDKTLDDLFWEADDSR"
     gene            complement(17956..18654)
                     /locus_tag="VGT87_000354"
     CDS             complement(17956..18654)
                     /locus_tag="VGT87_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813242.1"
                     /translation="MFDSKQNLAKSQWMFIIIIAVMDLVVLVATLLAKTSFQRYLVGG
                     VAVILTSFLILLVWGLKTAKKLQMRLDSKEVLDSKVRDDQKLPKYDERQKQILLKGYT
                     IGFWFMIVVIWLSLFISRFTEGLVSASFLFTLALWGGLAVQTTYCNLNGVSPFVDRRF
                     GKQGILMGMIATGMSLVTIGLAVREMMAGQLKMGSFFKAGGSGSLMVLGITLLSMGAS
                     IFYRHYLDAKEADE"
     gene            complement(18790..19650)
                     /locus_tag="VGT87_000355"
     CDS             complement(18790..19650)
                     /locus_tag="VGT87_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEQ3813243.1"
                     /translation="MTFTIMTDSTADLNQTWAEDHDIVLIGLTILCDGEVYETVGPNR
                     ISSDYLLKKMKAGSHPQTSQINVGEFEKVFREHARNNKALLYLAFSSVLSGTYQSALM
                     ARDLVREDYPDAVIEIVDTLAAAGGEGYLTILAAEARDSGKNLLETKDIVEAVIPRLR
                     TYFLVDDLFHLMRGGRLSKGSAFLGSLASIKPLLWIDEEGKLVPIAKIRGRQKAIKEM
                     VAQVEKDIADSTVIVSYTSDQGSAEKLREELLAHENISDVLMMPLGPVISAHVGPNTL
                     AVFVIGQNSR"
     gene            complement(19747..20265)
                     /locus_tag="VGT87_000356"
     CDS             complement(19747..20265)
                     /locus_tag="VGT87_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEQ3813244.1"
                     /translation="MKKRILLVDGYNMIAFWQSTRQLFKTNQLDQARNTLLTKLNHYA
                     HFENINIICVFDAQYVPGLRQRYDQYYISVVFTEEDETADSYIERMAAELNTAIHMVE
                     VATSDLNEQWTIFSQGALRVTARELEQRVHTVKADLDKMSRDIDLKTPKLRPFDQGQL
                     SQLKDFMSQLDR"
     gene            complement(20269..21015)
                     /gene="rlmB"
                     /locus_tag="VGT87_000357"
     CDS             complement(20269..21015)
                     /gene="rlmB"
                     /locus_tag="VGT87_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEQ3813245.1"
                     /translation="MEDKDTIETNDIVYGVHAVTESLQANTGNKLYIQEDLRGKKVDN
                     IKSLATQKKVAISWTPKKTLSQMTDGAVHQGFVLRVSAFAYTDVDEILEIAEQEANPL
                     ILILDGLTDPHNLGSILRTADATNVCGVIIPKHRSVGVTPVVSKTSTGAVEHIPIARV
                     TNLSQTLDKLKARGFWIFGTDMNGTPSDCWNTNGKLALVIGNEGKGISTNIKKQVDEM
                     ITIPMNGHVQSLNASVAAAILMYEVFRNRR"
     gene            complement(21062..21859)
                     /locus_tag="VGT87_000358"
     CDS             complement(21062..21859)
                     /locus_tag="VGT87_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813246.1"
                     /translation="MTTMQKTVSLLSLALLIGLLGTSGKAISVYAQDQHTDNVIAEST
                     ISQVSVEASMRGTEPYIDATVTTDQPVRQPTQATITLKDASDNTINSWVYTMAAQQRR
                     FTAWFDLTGQKSGDYHVTVTVHTQEKAVTGQSGTVHFDQNKARKTPTNMQQKDTSKAM
                     TNSVDVGTKAQTKQSANQEIDSTSNPFKSATNHRSTSLKRSTKNEKLTPTASNSQKNG
                     SNKTKMLVDKEEVKPTSKRGFPWVLSGLVVSLAAGLFIAIQKVSRRK"
     gene            complement(22055..22468)
                     /locus_tag="VGT87_000359"
     CDS             complement(22055..22468)
                     /locus_tag="VGT87_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEQ3813247.1"
                     /translation="MTNPVDVNLINGIALAFEGDAVYSYYVRRHLIFQGKTKPSQLHR
                     LATRYVSAKAQANLIQAMLEAQLLTEKEEDIYKRGRNTNSHTKAKNADIITYRMSTGF
                     EAIMGYLDMMGQKERLEELIRWCIEYVEKQQLISS"
     gene            complement(22461..23804)
                     /locus_tag="VGT87_000360"
     CDS             complement(22461..23804)
                     /locus_tag="VGT87_000360"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEQ3813248.1"
                     /translation="MIKIYDTMTRSLRKFVPLTENTVNMYVCGPTVYNYIHIGNARSA
                     VAFDTIRRYFEYTGYQVNYISNFTDVDDKIIKAATQAGVSPKELSDRFIAAFIEDTKA
                     LGVKPATQNPRVMDYIAEIISFVESLIEKDFAYEADGDVYFRVEKSEHYAKLANKTLS
                     ELEVGASGRTDAETALKENPLDFALWKSAKAGEVSWDSPWGFGRPGWHIECSVMATEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKTFANYWMHNGFVTVDNEKMSKSLGNF
                     VTVHDMLQTVDGQVLRFFLATQQYRKPINFTEKAIHDAEINLKYLKNTLQQPLTETAD
                     EQELKQFVIAFQDAMDDDFNTANGITVVFDMAKWINSGSYTEPVKSAFEKMLAVFGII
                     FEEEVLKVDIEALIAKRQEARANRDFATADAIRDQLAAQGIKLLDTKDGVRWLRD"
     gene            complement(23832..24062)
                     /locus_tag="VGT87_000361"
     CDS             complement(23832..24062)
                     /locus_tag="VGT87_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813249.1"
                     /translation="MNKIRVGLYVIMSLSILVFSITLVASLIFLLRQDMVKAIIYFIL
                     TLFWLIGLVINIKLLKRWNSISEKDLGYQDDN"
     gene            complement(24541..25122)
                     /gene="cysE"
                     /locus_tag="VGT87_000362"
     CDS             complement(24541..25122)
                     /gene="cysE"
                     /locus_tag="VGT87_000362"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEQ3813250.1"
                     /translation="MGWWKESIAIVKALDPAARNSLEVILTYPGIKALAAHRLSHFLW
                     RHHFKLLARMHSQFWRFWTQIEIHPGAQIAPGVFIDHGAGLVIGETAIVEKGVMLYHG
                     VTLGGTGKDCGKRHPTIRQGALISAHAQVIGPIDIGANAKVGAAAVVLSDVPEDVTVV
                     GVPAKIVRVHGQKDNRQIQSLQKQREVSYQLSK"
     gene            complement(25131..25883)
                     /locus_tag="VGT87_000363"
     CDS             complement(25131..25883)
                     /locus_tag="VGT87_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HEQ3813251.1"
                     /translation="MTKSNELDIRLRAFINAPDNFLDSVALVNAFHNFPVWAAKEPYV
                     IEVEGVKVTPVFTDKEDMFLFKEEQKSAQSQYWLERSALAVLEEVITSGAAGLVFNLK
                     KKGDFGNSTIFKSSDMIQFMNHYTTVLNTLMSDDNVAADTMEKVYLVPAFVYPKDDNH
                     YDRLFPTMSTPEGKSYVPAFSNLQSFAKWYNQDDFGGLFRKAEGVILTWTIDDIYQPR
                     NGENELDETFGVAINPFDDQQILVDWSELDKS"
     gene            complement(25876..28008)
                     /gene="pnp"
                     /locus_tag="VGT87_000364"
     CDS             complement(25876..28008)
                     /gene="pnp"
                     /locus_tag="VGT87_000364"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEQ3813252.1"
                     /translation="MSKQTFTTTFAGKPLVVEVGQVAKQANGATVVRYGDSTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFNGPIAGVQVGYIDGEFI
                     INPDKEQMEASLLELTVAGSKEAINMVESGAKELSEDIMLEALLKGHQAIQELIAFQE
                     QIVAVVGKEKAEVELLQVDADLQADIVAKYNAQLQKAVQVEEKKAREAATEAVKEMVK
                     AEYEERYAEDENLATIMRDVAEILEQMEHAEVRRLITEDKIRPDGRKIDEIRPLDAVV
                     DFLPKVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLAPEYKKRFLHHYNFPQYSVGE
                     TGRYGAAGRREIGHGALGERALEQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGTNYTVLTDIQGLEDHFGDMDFKVAGTREGITAL
                     QMDIKIAGITPQILEEALAQAKKARFEILDVIEATIAEPRPELAPTAPKIDTIKIDVD
                     KIKVVIGKGGETIDKIIAETGVKIDIDDEGNVSIYSSDQAAIDRTKEIIAGLVREAKV
                     GEVYHAKVIRIEKFGAFVNLFDKTDALVHISEIAWTRTANVSDVLEVGEDIDVKVIKI
                     DEKGRVDASMKALIPRPPKPEKKEEKHD"
     gene            complement(28288..29019)
                     /locus_tag="VGT87_000365"
     CDS             complement(28288..29019)
                     /locus_tag="VGT87_000365"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEQ3813253.1"
                     /translation="MMTKKLNIKVFSDGAVLETMLKDLQTGLVTGFTTNPSLMKKAGI
                     SSYIGFAKEVLAKITDYPVSFEVFADDLASMEKEAEKIASLGDNVYVKIPVTTSTGES
                     TCPLIQKLSAKGIKLNVTAIFTIEQTQAVVDHLTARVPAIVSVFAGRIADTGVDPMPI
                     MEEALRICRQKEGVELLWASPRETYNIYQADQLGVDIITCTTDLIAKLPLQGKDLEDY
                     SLETVQMFLKDSTSLGFKILEDAKH"
     gene            complement(29029..30408)
                     /locus_tag="VGT87_000366"
     CDS             complement(29029..30408)
                     /locus_tag="VGT87_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEQ3813254.1"
                     /translation="MEALLSFIRDILKEPAFLMGLIAFAGLVALKTPAHKVLTGTLGP
                     ILGYLMLAAGAGVIVTNLDPLAKLIEHGFSITGVVPNNEAVTSVAQKILGVETMSILV
                     VGLLLNLAFARFTRFKYIFLTGHHSFFMACLLSAVLGAVGFKGSLLIILGGFLLGAWS
                     AISPAIGQQYTLKVTDGDEIAMGHFGSLGYYLSAWVGSKVGKDSKDTEDLQISEKWSF
                     LRNTTISTGLIMVIFYLVATVASVLRNASVTEELAAGQNPFIFAIKSGLTFAVGVAIV
                     YAGVRMILADLIPAFQGIANKLIPNAIPAVDCAVFFPYAPTAVIIGFASSFVGGLLGM
                     LILGVAGGVLIIPGMVPHFFCGATAGIFGNSTGGRRGAMIGAFANGLLLAFLPAMLLP
                     VLGKLGFSNTTFGDVDFGVLGILLGRLGTSIGQIGIYLVVAVLTIVLVLPSFLSKSQE
                     AINNVSEEI"
     gene            complement(30435..30719)
                     /locus_tag="VGT87_000367"
     CDS             complement(30435..30719)
                     /locus_tag="VGT87_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEQ3813255.1"
                     /translation="MIKIVTVCGNGIGSSLLLRMKVEAIASSLGIDVDAESCDSNAAV
                     GKGADLFVTVKEFEDIFPEDAKVCIVKSYTNRKKIEEDLVPVLKEMSGKE"
     gene            complement(30712..32772)
                     /locus_tag="VGT87_000368"
     CDS             complement(30712..32772)
                     /locus_tag="VGT87_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEQ3813256.1"
                     /translation="MLSHELIRNYQLFSKYKGHSLEAFESILKTSKRHILADIAKIND
                     TLSLYQLPLIALDRQLVYPPDLTEKDLLNRMLPTLDDYLFQDERLDMIIIYIMMAKEF
                     ISINHLESLLRLSRNSVIADLNLVRDRVQAFQVTLAYNRQDGYFFEGEPLALRRLLES
                     AVSSLLQVISGSWVFSYLLHELGLPDQTKVMAATLEELSRENHLTFISEKLRDLIYFF
                     CLLAHRPFSRNVRAEAVDTFPLASPAVETMVDQLLVNFPSLTEEKYLVQSRLLGCIQG
                     DLELVFQQPIYDIMEEIINSVAVNTGLSITDTPELRQNLYSHLLPAYYRLYYDINLTN
                     PLKEQIKQDYESLFYLVKRSLSPLEKQLGKSVNEDEVAYFTIHFGRWLQAPKKRPSNQ
                     LVALSVCPNGISSSLMLEATLKELFPQLQFIRIHQLDKIKLLDPASFDLIFSTVAFDC
                     AKPVYVTQALMGPVEKMMLKKMVCDDFHLPLSEQFALDDLLSIIHKHTTITNKEGLVS
                     DLSRYLIGNHLTIEKGGLGLLDLLTADFIRQADAVSDWQEAIRLAAQPLLEHQMIETS
                     YIDGMIDSVNELGAYIVLAPKVAVPHAAPEKGTRQLGMSLLQLKEPVSFDLKQEGDPD
                     KQVQLIFVLSAVDSSSHLKALQELSLILDDDEHIEQLIEAKNTEEIMSLISHMIEKGD
                     ESHD"
     gene            complement(33124..33393)
                     /gene="rpsO"
                     /locus_tag="VGT87_000369"
     CDS             complement(33124..33393)
                     /gene="rpsO"
                     /locus_tag="VGT87_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEQ3813257.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNSHIK
                     EHKKDHATYRGLMKKIGHRRNLLAYLRRTDVNRYRELIQSLGLRR"
     gene            complement(33569..33769)
                     /locus_tag="VGT87_000370"
     CDS             complement(33569..33769)
                     /locus_tag="VGT87_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813258.1"
                     /translation="MTMLGYRLSAEVLGSAILPLLAGYLIRQAIAQVYSDLKTAFWLF
                     GLASSVAYGGLVKAKRAHSMTR"
     gene            33895..34164
                     /locus_tag="VGT87_000371"
     CDS             33895..34164
                     /locus_tag="VGT87_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEQ3813259.1"
                     /translation="MTQNVCQQNITYIVKERFASYILKAQANQEVHLNFILLANRFET
                     SDRHLKHVLKQPIFQRIIERICFKTYRIADLDALTQLRPLRYAVH"
     gene            34265..>34858
                     /locus_tag="VGT87_000372"
     CDS             34265..>34858
                     /locus_tag="VGT87_000372"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEQ3813260.1"
                     /translation="MSAQDKLIKPSHLITMDDIIREGNPTLRAVAKEVSLPLCDEDIL
                     LGEKMMQFLKHSQDPVMAEKLGLRAGVGLAAPQIDVSKRIIAVLVPNLPDKEGNPPKE
                     AYSWQEVLYNPKIVSHSVQDAALSDGEGCLSVDRVVEGYVVRHARVTVDYYDKEGQQH
                     RIKLKGYNAIVVQHEIDHINGVLFYDRINAKNPFETKE"
BASE COUNT        10884 a         7114 c         5942 g        10918 t
ORIGIN      
        1 agatgcaaga aattaccact agacaaaaca atgtgcttgc gtcaacaact ataaaaaata
       61 gcaaaaaatc tttaactaat accttcatga gtacaatcca acaaaggata agaagtagaa
      121 ataatagttc tatacgagct ttgtaatcac ctaaccatct ttgggtaaca aaactcaatt
      181 tatcatgtat ggtcttaaaa tagtatatta cactcattat aaggggaaac agataccatc
      241 ccaaattatt aaaggcttga ttaacatcta gcatttctcc ataaagttca ctaccaagta
      301 ttaccagcaa aagtaaagaa aatagtatca atgaacgtct aacaactgat atttgttgcc
      361 taaaggacct ataatatagc actataacta gtaacggata caggtgtaaa atagtaaaaa
      421 ccatcaggaa taaaaaatta aataaaatgc tattaacaaa aaggctagtt atagaaacaa
      481 aaataattat taacaagccc ttatctaact tatctttaaa ttgtttgtgc ccatataata
      541 ttaaattagt aatctcaacg gtataacaat agagcaatat aatctgtagt ccactgctta
      601 aaaagagagg agttcctctt gtcagcacag atactaagta ttgccaaatt gttaaggaga
      661 tgatagaaat aatcctttta aaaatatcac cttgtttttg gcgtaatatt gttgccagcc
      721 aaataaaagc tgtatcaata agggtcatta caagagctac tttttcagag aaaagaagag
      781 acaggttctc tcgattatag accaaaatta acaaggctat taattgttgc aacaagatta
      841 ttgcagtccc aaagaactta ctctttgtta tgttttttat tcttatcata ttatttactt
      901 aatcgttaaa gttaaaagat ttttaaaatt aatttttgat tatggatgtc actgaccgca
      961 atgtcaatac ataaaatgtg ccatatatta tcaagaagaa aagcagacaa agcaataaag
     1021 ataacattat gatattgcct gctaccaatg tatccatgaa taaatattta tagtaaataa
     1081 gcaacatacc accatttaca atcccaatga atacaggaat aaggaaaagt atacctgttt
     1141 catatcgaag aatccgatga atcatttttt tactataacc tagcttactt agatagccat
     1201 attcttcttt attttccatg atttcgatga ggcttgtaaa gtacagaata ctacctgtac
     1261 aaataataaa gagtatggat aggaaagtta taaagatata agtcgcaata ttagcagtct
     1321 ttactgtgta ttccttacta taactactga taacatcagg aaccatacta aactgattgt
     1381 aaaatgcttc acttgaccta atcgaatttc cattaaaagt cctaattgtc atttctttct
     1441 ctggaaaaca agaactaagt ttagcatata atttatcaga aacaaccaag gtcgtgacac
     1501 ttgtagaaaa actaaaaaca ttatgcgttg atacttgttt tacagtaaca tcatatgcat
     1561 ttcctaagtt aaaggttttt ccaaggctaa tttctgttgg ataataattt attaacaatg
     1621 ggagtgactt actaccttca atagaattta ttctcccttg agctttcatt aactctttat
     1681 aatcactgta attaattata tcaaaataag gaattgtttg cccagattct gtgatagtta
     1741 cttctgggtt agtgttcaat ctcaataggt caacatcaac caagctcact tggtcgttct
     1801 ctaaaatagt tgtaacattg acttgaccgt tttctgattc aacattatat tctatggcag
     1861 atggagtcaa cctctctatc ccattaagag aataagcata gagcattacc atcattccgg
     1921 caactgaaac ggttactgtt aaaagacctc ccaagactgt taataattta ttttttgttg
     1981 caatacgata gttaaatttg ggatagatga tattagacag aggacggtat aacctttttt
     2041 tattttcttt taacaacgaa actacaaaag ccaatccata tctgatggtg aacactgttc
     2101 ctaaaacaac taagacaatt atcaatagag aaaccggtac catccctatg cttaatagca
     2161 tatttcgtgt ggatgaaaac actgtcgcca aacataaaat atatgacaat agcaatgcag
     2221 tgatagcagt gatagcagta atagctctga cttttcttat cttaattttt ttctcagcct
     2281 ttcttgagaa attaacaaat tcaatcaaag actgtttact aatgataagt ccatttgtga
     2341 tgacattaat cagaaaaact acaactaata ttcctaaaga ctctataatc tcattagctg
     2401 taataaacca ctgaacttcc aatgttaatt ccaatagatg agtaattgcc agtacagcta
     2461 aaaaatataa agtcggtccc agcaataaac ttaccaagta actcagaact agaataacaa
     2521 gattttctaa agttagtaat tttgttaatt ctctttttga aaatcctagt attgctaaga
     2581 ctccaagctc ttgactacgt ttttttacaa agaaattatt gaagtaaatt aagaatacca
     2641 ccaaaaatac aatcaaaaaa atcaatgacc cattagcgat aactagagct tgaccactat
     2701 ccccaatttt ttctgtaatg attttatctg agataaaatt tagaaaagct ataaaaatac
     2761 ttaataaacc aatcgtagct agaaaataga tacgatataa cgaaaaattt tttttaatgt
     2821 tagattttgt aattgaccaa atcatatcac tctcctctat cggataaagc ttgactaatt
     2881 acatcgtaaa acgactctcc acaatctttt ttatcaatct ccatgtgaag tttaccatct
     2941 ttgagaagta caactctatc cgcataagaa gctgataaaa cgtcatgagt aaccattaaa
     3001 atagttacct tttctgtttt attgatttca ctcaaaatca tcattgtttc ctcagaagat
     3061 ttcaagtcaa gtgctccagt tggttcgtca gcaaaaataa tctctggttc tttaatgatt
     3121 gctctgataa tactgaccct ctgtctttga cctccagaaa gttgatacgg atatttcttc
     3181 aactgttcat ataaattaaa tctttttgca tagctattta tcaaatcatc aatctttgaa
     3241 gaatcaatat tttttagaga caaagcaaca gaaatattct ccctaatggt tagactatct
     3301 aacagcatat aatcttgaaa aacaaaacca atatcatttt tcctaaaact agttgcctcg
     3361 ttatcagaaa atacactaat ttctttgtct gcacaataaa tactcccact atcaatttta
     3421 tcaataattg aagtaacatt taaaagtgtt gtttttccac taccactacg tcccatgaca
     3481 gctacaaatt ctccttttct aacagtcaaa ttgatatggt ctaaagcata attcggttgg
     3541 tttggatacc ttttgcaaac atttttcaat agtaattgat tcattttatc ccttcctcct
     3601 gatagttgtt catgtacgaa tcatataaat cacgatagta ctcattattt ttatataaac
     3661 aatgatgact accatagtca ataattttgc cattatccat tattaaaatc ttatcagctt
     3721 tttcaactgt attaagtcta tgggtaacca aaaccctcgt acaatgtaaa gcctcaacat
     3781 ttttttggat tattctttca gtttttacat ctaaagctga tgtcggttcg tcaagaacta
     3841 agattctagg ggtagttaca agttctcttg ctaaagaaat tcgttgtttt tgccccccag
     3901 atagtgatgg attatctcga aaaagtggag tatgaaactt cattggcata cttatgatat
     3961 cgtcatatat tgataccctt ttacaagttt caataacctg ttctcctgaa acagattctc
     4021 tccctagcga aatattatac atcacatccc catcgaatat gggagaatct tgagatacaa
     4081 aagccgatat atccctaaaa tcttttttat caattttaga gaaggtaatg ttctgaggag
     4141 attgataaaa actgataaga tttgcccttt tccctctccc aatgcttaat cggttttttc
     4201 aagactattc caagcaattc gcaagtgaac ttcattcttt catcattcag ggtagctata
     4261 ttatcaaata atgaggttgt ctctcctaga tttccaatta ctgttcctaa agaattatat
     4321 acatttccac tggtatccat tgaaaagtaa ggaatatcac cattcatcaa atctttaatt
     4381 tcactaacaa caataaattg ttcaatatgt tttgactcat gtagtatcga aaataaattc
     4441 tccctctttt tttctgagaa aagatacttt ggattcgtag aggcttgtaa aaattttcca
     4501 tagtcggaag tatttctgaa tatggctcta gttttcaatt ttgggaaatc ttctaaaatt
     4561 tttttaacct ttgcacgttg cgacatgaga aatgtatagc attctctaaa atctgcaatt
     4621 acctcattag catagtcgag aggttccacc actttcccat ttaatgtcgg aatattataa
     4681 ccatcttcct gaaaatagtc agttttcact aacttcatat ctccacgatt tttatttatc
     4741 agttcatatt ttcctttttt tctttttcca ccttttccac ttattccaga aacgttagga
     4801 gcgctgtttt tctcaaattg tacaggaaat aaaccgtttg aaagtgatgt ctcataaatt
     4861 ctctgatagg tgtctacagt aatcccttca aaattctgat tttgtacatt caactttctt
     4921 tgaaaaaagg tttcaacgtc aattaaatat aaatctccgc ctgaaacaat cacattttcc
     4981 atatgtaagt ccgtaataga aaatatttca gaaaatgctg ctaaaacacc atacttaaag
     5041 taaattttcg ctacttcatc tttattggaa cttgtataag ctacaccaag ttgccaacaa
     5101 taatctgctt taactaagct tttaggaaga ctgaatgaag gtatattact agatttcaat
     5161 aaactagtaa gttctaacaa gatactgtgt gaatgataac ttttaggctt gtagtaaaca
     5221 gatttctctt taaacgcaat tttaacaact gtttgctttt tagagtgact gtcaccaagt
     5281 gaaaaggcaa cattgcttag gggctcacta atgttaaagc acgaccttat ctcagataag
     5341 tcttctaaaa atctatcaaa taataaagaa tatgattcct ctatcagtcc tacttcttta
     5401 ttaatttgtt ttagcaaata cggatacctg tcaaaaaaat gatttattcc gttttcttct
     5461 cttatctgct gtatatactg ctcatatgcc tctgacttat tttcaatttc cttggaaaaa
     5521 tgatttatat ctaaaactag tgttttcatc gaaaggaaca tgatcgtttc aaataactga
     5581 ttttcaaact cactataaaa taacttatgt gtctttttcc ttaacacttc actggtttct
     5641 ctaatagaag tatctaatat gtcatagtca aaaattgagt taacgtattc gtaaaactga
     5701 tggttctgat attctaactt taattcatct ctttttatca tttttttcct accactactt
     5761 aaaaatcctg cctagactga gtctaaacag gaaattttta gtctgcttgt ccagattgtt
     5821 ttaattaaca acaaacaaat actgagttcg gacagtcatc agtaatagtt gcaaaccaac
     5881 cggaaccttt tttaccacca gctacttcca taagttcttt ttcagaaact tcttcgatag
     5941 cattagtcaa aatatccttt gaattttcca taaaactcat tctcctttcc atcagtatgt
     6001 agtgactatt ttctacactt ttattgtaaa taatacgtct tcattttcaa tgagtaaaaa
     6061 gtgcaaaaat gactttttca tattttttag taagattttt gagatgtcac ataagaggtc
     6121 actttataac aaatagccct ctgatataaa aaaaaacaaa actcgttctt tacagctatt
     6181 ttacaatcta atcgcttctc gttataacat caatctctaa tcagaaaatg gttactttac
     6241 ttatttaatc aggtcaggag tttgaatgac agaagtcaaa tatctgaaaa aggatgttaa
     6301 ataaaacttt caaaacccat tttcatcaac taaaaacact tataacccga tcttacaact
     6361 aatggattac catcaacagc aaataatact atgcttgaaa aatgtcaaca tcatctactt
     6421 tttgtgacta taattcagta aacgaacatg atgtgtaaaa atgtattaaa aaagtatctc
     6481 aaaatcattc aatcacttga gtttctgcta cttttttata ccagtaggca ctcttcttag
     6541 gataacgttc ttgagtctca aaatcgatat agaagagacc gtaacgtttt tcatagccat
     6601 ttgaccatga aaagacatcc atcagtgacc acataaagta tcctttaaca tttgcaccat
     6661 cagaaatagc atcagaaata acttctaagt gttttttcac ataatcgata cgtccatcat
     6721 catagacagt attatctaca aactcatctt tgtaaccaag accattttca gtgatataaa
     6781 ttttcttgta gttaggataa tcagctttaa cacgcatgat ttggtcatat agtccttgtg
     6841 ggaagataat ccagtcccag tccgtttttg gaacatcaac gggtgctttt cttcgaccaa
     6901 cacccttgat ttggtattta gagctgcctt tttccccttt accattatga atgatttcag
     6961 tctcaccatc aaaagcttgc atccaatcac tcatataata gttaatacca aggaagtcat
     7021 tcaaatcttt tgcggcgtct agtgcagtaa aatcttcttc acgaagatca agttcaccac
     7081 cattcacctc aaggatatgg ttaacacctt ccattgtttt atctgaatac ttacctagat
     7141 aagttgcatc aagaataaat ttattatgga tgatatcttc cagttcagct gctctaacat
     7201 catcaagatt gttagcgtca aatggatact tagttggaag agcatggaca acgccgattt
     7261 cacctgaata accactatct ttaaaaagtt tgactgcacg agcatgagag accatcatgt
     7321 tatggtgtga ttggaaaact ttagcaagat catattggat acctggaggg aatttaccaa
     7381 ctaagtattg accatcgcca attggtccaa tttcgttaaa tgttgtccaa tagttaactt
     7441 ctgagaattc tttaaaacaa aattctgcat aatttacaaa atgttcaatg ttctcacgat
     7501 tgaggaaatc accatccgag tggagagctt ctggtgtatc aaaatggtga agtgtaacaa
     7561 aaggctcaac atgacgctta tgacactctg caaaaagatt gtggtagtat tctactcctt
     7621 tagggttaac ttctcctttt cctgttggaa aaatacgaga ccaggcaata gagatacgga
     7681 tcccattaac accaaattct tcactaagtt tcaaatcgac agggtaacga ttataaaaat
     7741 cacttgctgg ctcagctgtg taccaatagt tgtcttctaa gtatttatcc caagctactg
     7801 gtcctttgcc atctgtatgg gtagcacctt cagcttgata agcagctgta gcaccaccaa
     7861 aaataaaatc tttaggtaat gtttttgtca tgatatacct cttctaaatt ttcatttcac
     7921 tataaaggaa gattgagtac ccccaacctt ccctagtgaa atattattct tccatctgtt
     7981 ctttaacgaa gtccaatgca ccttgaccat cgcgagtcaa tttgatgtat tgagcccctt
     8041 ctgttttagc taatttaata tcaagtttat cagtttccac tttcatgtct tcatagttag
     8101 aagctacttg aggtgcaagg ataacaagtt gatattgtgg taaaatctca cggtgagcac
     8161 cataacttcc tgctgctgca gtgacaggtg caccatattc tttagctgct ttattcaaag
     8221 cgtttgcaag caaaccactt gtaccaccac cggcacaaag aacgagaaca ttagtttgat
     8281 cagtgatttc tttggtttct ttagctgctg atttttcaag aatagcatca gcttttttag
     8341 tatcaaagtt tgctgctact ttttctttga ggacatctga tgaattcgta cctgctgctt
     8401 cttcagcaag aatttgctca tcatatactt tcaagaatgg gtagtagatg atgacatcaa
     8461 ctacgattaa caaagctgcc aaaatgaatg ctagcacttg gaagtttgtc ccaagaataa
     8521 gacctaatgg agctggtgtt gtccaaggta agtttgcagt gaaactgttc attccaagtg
     8581 tgtcaataaa gaatttgaaa atccatacat tgacaattgg tgctaggata aatgggataa
     8641 agaaaattgg gttcaaaaca agtggagcac cgaacaagat aggttcgtta acaccaaaga
     8701 atgttggaac aactgatgcg cgtccaatgg ctttgttacg ttttgatttt gttaaccaca
     8761 tgaacatgaa tggaacgaca agtgttgcac cagtaccacc catggtaacg atgaacattt
     8821 gagtccctga agtcaagatc ttatcagcat gttcaccagc ttgaagtaat tgaaggttgg
     8881 tttcagcatt tgcgtaagta attgctgcaa tggctggttc aacaatagat ggtccatgga
     8941 taccaacaaa ccagaagaga gcaaatgcac cgaagataat agtgattcca acataaccat
     9001 ctgctgcttg gaacaatggt gcaagaagtt taccaattga ctcggcaacg ttaacgccta
     9061 aagtactatg aacaatcaat tcaatcacat aaagcagtac aactgataac gtaaatggaa
     9121 tcaaatcttt aaaaacttga gagatgtttg gtggcacctc ttctggcatg cgaatagtga
     9181 cattgttttt tacacagact ttatagacat ttacggtaat aaaagctgcg atgaaggccg
     9241 ttaacaaacc ttttgttccc ataaatccag ttaagaaacc accgtctttt gctggatttg
     9301 ctgccattag tagaaaacct acaatagcag caagcatggt tgaaataaag ttgatttggt
     9361 tagtgctaga caatgagcgg ttcattgaat cggttaatgc tttggctgtc gtaccagcca
     9421 caaacaaacc taaaataccc attgagtagt tgtatggcgt catcaagaga ttctcaatat
     9481 ccttactcca atggaagccc catgcatttg gaacataggc aatcaagata aagatacttg
     9541 aaaacaagat aacaggcata ccagcgataa aaccatcacg aatagcgcga agatagatgt
     9601 ttcgagaaat tttctcgaag aaaggtttcc ctttctcaat ctgagcaatt aatccattca
     9661 ttattttgct cctctcttat aaagttcaat catgtgtttc attaaatctt gtaataaaat
     9721 ggtcgtcatt aaatgatctt gaccatgcat taaagtgaca ctataagcta agtcatctcc
     9781 agctgcttct ttttgtagta gacttgtctg agcattgtga gcatcattca agcagtcact
     9841 acctgcagcg attaattctt ctgctttagc ataatcccct tcttgcgctg ctttaagcgc
     9901 ttccaaaaat tttgaacggg catcaccagc ataggctacg atttcaaaac ctaaaagagt
     9961 agtttcttct ctattcattg tagtttcctc cgaaaataat gttacatttt ttcagtccaa
    10021 ggacttgcag ttttatctag cactttgttt aattcgtcaa tatttttgaa accttctgta
    10081 cgcaaccatt cacgagctgc ttgtggtcct tcttcgatat agacttttac agatcctgcc
    10141 caggtagcac gtccacataa gacaccgttg aatttagcac ctgattctgc tgcaaacacc
    10201 aaagtatctt ggaacaattt ggcagaaaca cctgcactca agtaaatata tggtaaatca
    10261 gtagaagctt cttggtcacg gaaagcttga gctgcttctt cttttgtaaa gagaacctca
    10321 ccatctgcaa agctttctac aaatttcata tttactggaa cttcgacttt caagacgtca
    10381 acaccaaagc gttcttctga gaagactttc attgcttcat taactttatg agctttaact
    10441 tttgcaaact cagggctagc attgtcagag attttctcat cataagttag aatttctaag
    10501 tagaatggaa tatcttctgc acggcattca gaaccaatac gttcaatgta agcttttttc
    10561 tgctcgttga tatcttgatc tccatcaata tcatagtaaa gaaggaattt aactgcttct
    10621 gctccagctt ctttaatacg ttttgcagac caaacatcta agcaatcagg taagcgacta
    10681 gttgttgttg catcgtatcc tgttttctca tatgccaaca acaagcctac ttcttctgaa
    10741 cgaacacgac ttgctggcaa gccatattca ggatcaagta aaattgatga tgcaaatggt
    10801 gttagttctt cagaaactaa acttttaagc tcttcaattt gttctactgt tggctctttt
    10861 gtttgatgtt gagccatcat acgtttgaga gctccacgtt ggtcaaaagc taaagcagaa
    10921 ataacaccat ctatacttag tttttccatg cttttacgtt tgttttcagt taaagtaatt
    10981 gtcatttttt atacctcttt gactttaata gattgatata acttatcgta atttgccatg
    11041 ttaacatgcc cagttgtttt ttcttgcgca tttaacatcc ctaagacatt tgcttttgtt
    11101 aataaagctc gatcatcttc atcattcgct aaacctgaag caatacctgc cactgtagaa
    11161 tcccctgaac ctacagcgct tacaattttg attttaggaa tgtcaacgtt ataaaatgta
    11221 tccttgtgct ttgcaaatac accatcagca ccaagagaaa cgattatcca ttcaattcct
    11281 tcaaatagtt catcttgtaa gacttctttt aaggcttcaa aatcttttgt catttcttta
    11341 cctaaaagtt gagaaagttc ttcaagatta ggcttgataa ctgttggttt actatcacct
    11401 tttagtactg cttctagagc atttccagaa caatccagaa ccgttttctt accattaaga
    11461 ttggcaatct tgattaactt ctgatagtaa tcatctggca taccagcagg caaactgcct
    11521 gacattgtca ctacgtcaac atcattcagt aaatacttaa attgatgagt aaatccatca
    11581 atttcatcta cacttaacag cggtcctttt tctaaaatct ccgtttgatt accttcgtgt
    11641 aaaatggcta tacaagttct agtatcacct gaaattttat agaagcgact taaaatcgaa
    11701 tcaggaagat gattaatgat aaaatcaccg ctttctccac ctacacaacc tgtagctttg
    11761 actgtctcac caaagtcatt tagaactcgg gaaacattca agcctttacc accgggtgtc
    11821 tttgtaacgt ctacaactcg attgacagta tcgcatttta gctcatccaa agggtaggag
    11881 acgtcaatag ctggatttaa agtaactgtt aaaatcacaa tagctccttt ttctatatat
    11941 ccttatcata attctttcaa atattagtcg tgatattcac cacggtccca tttttctaga
    12001 aattcatcaa agaagtgtgg gttgttttga tcttggctag catgttgcaa gctatccatt
    12061 ttttcaatcc attttttatt ttcttcagtt ggtttgtatt ccgcggctaa gaaagcatcg
    12121 ataatatcat tcatcaataa accaccgatg atttttccac caaaaccaat aacgtttgcg
    12181 tttagctctt cttttgagta gatagcagat gtcatatctc taacgagtgc tgaacgaatt
    12241 ccaggaactt tgtttacggc atttgtaata ccaacaccag taccacagat aacaacacct
    12301 agttctgctt caccacttgc tactgcttca ccaacttttt taccaaaaat tggatagtgt
    12361 gtgcgaacat tatcatacgt accacaatca ataactttat gaccttgtga ttttaggtaa
    12421 tccacaacag cgattttttc ataagtaaca atatggtcac aacctacagc aattttcatt
    12481 tttgttctcc ttgttttaac ctatttcaat caattagcac attttattaa gcatgtcgac
    12541 acgaacttga tgacgtccac catcatattt accttcgaca aagcctttga taatgttctt
    12601 tgcaattcct ggagcactaa tttcagaacc aagcgtgatg attcgagcat tgttgtgtcc
    12661 tcttgtcatg tatgctgaac gctcatctga tacttcagct gccaccattc ctttgatttt
    12721 agtagctacc atgaaagaac caacaccata tgcatcaatg acaataccta agttatcttc
    12781 ggatgcatta acttcttttg caacggctaa agttgtatct acaaagtcac gtacttcgtt
    12841 ttcactaaca tcaacgactt cgtgtttacc ttctttaagg taatctttga taacttcttt
    12901 aagttcttga cctgctttgt cagcaccgat aataattgcc attttatttc tcctatgatt
    12961 tagaataagt aaatgtggaa aacctacgta gatagttaat taacgattta caacatcttt
    13021 tgaaataacg ctttcattat ttctatgacc taatcataca cccaccaaac ataaaagtca
    13081 acattttttt gtttattttt ataaaaaata aacaaaatat gtttattttt ttcattttta
    13141 cgttattatc ctaatgtttc ttgtgtttct ttttcatttc ataaatccct ttgacagcca
    13201 ctcgtaacct atcatatatt acgagaccct tatctctatt tattgtaaac gttttattta
    13261 aaacattttc tattttgtta atttttgttt tttccaatgt ttatgtttgt tttgtttgat
    13321 ttttttattg atattatgtt atgatacctt taatagttat accctaagac actgatagga
    13381 gataagaatg aaaaagaaag agcgtcatga aaaaattctc gatatcttaa aagttgacgg
    13441 ttttataaaa gtaaaagata ttatcgatga aatgaacatc tcagatatga ccgctagacg
    13501 agatctcgat acattggcag ataaaggatt acttatcagg acccatggtg gtgctcaata
    13561 tttggattat tcttcagcaa aggacgaagg tcacgagaaa acacacactg aaaagaaggt
    13621 cttacagact accgaaaaaa aacaaattgc acaaagagct aaaaacatta ttaaagatgg
    13681 cgaaactgtc tttataggtc cagggacaac attagagcat ctagctgtcg aattgaaaaa
    13741 tagaaatatc agggtcatta caaatagcct tcctgttttt ttaattttgc acccaagcac
    13801 tactattgat ttgattttaa ttggtggtga atgtcgtgaa attactggag cttttgttgg
    13861 tgctattgcc attaataatc tagaatcatt aactttttca aaggcttttg ttagcgcaaa
    13921 tgcagtttac aataattcta ttgcgacgta cagtgatctc gaaggagaag ttcaaagggt
    13981 tgcattgaat aatgctgttg agaaaatcct tctagttgat agtaccaagt ttgaaagtta
    14041 tgacttctat aatttctacc aactagatca agtggactta attgtgactg ataaaaatgt
    14101 gacaactgat atcatagaaa agtacaagaa attctcaaaa atcataatcg ctgacaagta
    14161 aaggacttac tcatattaaa aagcacttca gggtttagat atatcccctg tcaagtaaaa
    14221 agggagcata tctgaaagta gtgtttgtat tataaagaag tttcttgcta acttattttc
    14281 tggttttttt cagacttatc agtcttgtac tgttcctttt tctgatgaat aacgtggaaa
    14341 taggcagaca gctatatatt tcacctctag aatggttgtc attactgtca tcaagacatg
    14401 cttgttcttt atcgtatgga gtgaagcttt cttttagaca tcaacatacc tcttaagtag
    14461 attttggttc gttaccatgt ctacttaaga ggtgtcttca aagcgcttta aaacgttgat
    14521 ataacagtat attaacgttt tgagaattgt gaagctttac gagctttttt aagacctggt
    14581 ttttggttca ctccattatt taaagtcaaa tccctttaaa atcaatgttt ctaaaatgaa
    14641 aaatcactaa aaatcttatt tatagacaaa tatttaaacc taaacggtat caaaaataag
    14701 tttcggagct tatcaaataa tgaaaatctg tctcaattac cgagtctttt ttattgactt
    14761 ttttgtatat atgatataat taaataactt acataaaggg gttacataaa atgactacag
    14821 taaaaaaaaa cagagcggtt acctttcaag ctaataaaga attggtaagc gaagcaatga
    14881 cagtattaaa caaaaaaaat ttaaccttat catctgcttt aagattattc ctacaaaatg
    14941 tcgttgtcac aaacgaggtt gacttattga cggaagagga gttagagaaa gaaaaacttt
    15001 tcaagcaatt tcaagctgaa atcaacaaaa atattgagga tgttcgtcaa gggaagtttt
    15061 atacctctga agaagtgaga gctgaacttg gactataaga aatatcagat tatctatgct
    15121 cctgatgttt tagagaagct aaaagaaatt cgtgattata tttctcaaaa ctattcctca
    15181 acatcaggtc agcgtaaaat ggagcaaatc attagcgaca tagaaaaact cgaggttttc
    15241 ccagaagttg gttttgaggc tgatgaaaaa tatggttcaa aaatcagcca ttatcacagc
    15301 actaaaggtt acaccttaag taaagattat attgtcctat accatatcga gggggaagaa
    15361 aatagggtcg ttattgatta cttgcttcct actcaaagcg actatataaa gttattcaaa
    15421 taacacgtta aaagccttag cagtcttgct agggcttttt tggtagatta ccaattaact
    15481 aggagcaaaa tgagttcggg ctgtttagta gatattccac aaagaagcca aagactacga
    15541 agctaggcta atctacctta aggcttcagg aaacttggag gagttcgcca agccaactca
    15601 taaaacttat agcgagatat tcgagaaatg gtatcaagcc tatcaagata cggtagagcc
    15661 aaccacggct tctcgtactc ttgatctgtt tcgacttcac attctgccag tcatgggaga
    15721 attgcccatt aacaaaacct cccctttaga ctgctagaac ttcatcaccg ataaagcaaa
    15781 gacctttaag aatatcaagc aaatcaagtc ctacactgaa aaggtctttg attttgccat
    15841 caagatgaaa ctgctcaagc ataaccctat ggctgaaatc attatgccaa agcggaaaaa
    15901 gacacgaata gaaaactatt ggacggttca agaacttcag gagtttttag ccatagtact
    15961 tcagaaagag ccttacaagc actacgcact atttcggcta ctagcttata gcggccttaa
    16021 ggaaaggcga gctatgcgct cttaaatggg ctgatataga ctttcagagt gagacactaa
    16081 gcgtcaataa gagcctagga cgattagacg gacaggcaat cgaaaaaagc actaaaaatg
    16141 atttctcagt aagaaagatt aagctagaca gcgaaactct ttccattctt caagaatgga
    16201 aagccatctg ccaaaaagaa aaggcacaac tagcagttgc acctctttca attgagtctg
    16261 actttatatt tacatactgt actagagaag ggagtattga accacttcat gcagactata
    16321 ttaacaatat cttatcaaag gtcattaagc aacatagtct taaaaaaata agtcctcacg
    16381 gcttcaggca tacccatgca accctgatga ttgagattgg agttgaccca gtaaatacag
    16441 ccaaaaggtt aggacatgct attagtcaaa tgactcttga tacatacagt catgctactg
    16501 cagtctgaga ggacaaatca atcaaacaat ttgctaacta tctcaaaaat aactaaataa
    16561 acgtattaaa ccaaatcaaa agttttccga aacggtatca aaagcaaaaa acgacaaaaa
    16621 aagcaccttg caagagactt gcaaaatgct tgtaatcgtt gatgtaaagc tgttcttaac
    16681 gttttgagaa ttgtgaagct ttacgagctt tcttaagacc tggtttttta cgttcaacca
    16741 tacgtgcgtc acgtgtaaga aggccagcgc gtttcaatga atcgcggaag tctgggtcta
    16801 cttgaagaag cgcacgagcg ataccatgac ggatcgcacc tgattgtcca ccgtaaccac
    16861 caccaacaac gttaacgaaa acgtcgtatg aaccttcagt tgatgtaact gcaaaaggtt
    16921 ggttgatgat caaacgtagg tcagcatgtg ggatgtattc ttctacatct tttttgttaa
    16981 cagtgatttt accagtacct ggaaccaaac gaacgcgtgc aacagcgttt ttacgacggc
    17041 cagtacctgc atattgtgct tgtgccatta tttaatgctc ctttcctctt agataagtcc
    17101 tgagatgtca agtacttctg gttgttgtgc tgcgtgagtg tgctcaccgc caacgaagac
    17161 tttcaatttc ataccttgtg cacgtccaag agtgttatgt ggaagcatgc ctttaactga
    17221 tttttcaatc aagcgaactg cgtttttaga gcgaagttca ccagcagtga ttgatttcaa
    17281 accacctggg tacattgagt gagtgtagta aactttatca gttgcttttt taccagttaa
    17341 tttaactttt tcagcgttga taacgataac aaagtcacct gtatcagtgt gtggtgtgaa
    17401 agttggtttg ttttttccgc gaagtacgct agcaactact gcagaaagac gtccaagtgg
    17461 cacatcagtt gcgtcaacaa cgtaccattt gcgttcaact tggcctggtt tagccatgaa
    17521 agttgttttg ttcatgattt ctcctatacg aattcgtaat atttgtttac aaggtgatgg
    17581 gtgttccgtc ccatcgaagg tatttggaag gttccggggc ctttcaaatg gggtaaacaa
    17641 taccgcctac tatgatatca aacttaagag gctccagtca agtgctttag ctgattttat
    17701 tggattattt tttctttcga tttaggagaa tggcagagct gctctgctgg tgttaacgag
    17761 aatcatcagc ttcccaaaaa aggtcgtcta aggtcttatc aagcactcga caaatggcaa
    17821 tgcaaagatt aatcgttggg ttgtaatcac ctttttcaac agccgcaatg gtttgtctgg
    17881 agacgcctac caaatctgct agagcttgtt gagacaaatc tttgcctgca cgcgctgctt
    17941 ttaatttgag gttcttcatt catctgcctc tttagcatcc agataatgac gatagaaaat
    18001 actagctccc atactaagga gcgttatgcc taacaccatt aaagatccac tgccgccagc
    18061 cttgaaaaag cttcccattt taagttgccc tgccatcatc tctcttaccg ccaaacctat
    18121 agtgactaag ctcattcctg ttgctatcat ccccattagt attccttgtt ttccaaagcg
    18181 cctatccaca aatggcgata ccccgttgag gttacaatat gtcgtttgaa ctgccaaccc
    18241 tccccataaa gccaacgtaa agaggaagct tgcagatacc agtccctctg tgaatcggct
    18301 aataaagagt gacagccaga taaccacaat cataaaccaa aacccaatag tgtatccttt
    18361 gagaagaatc tgtttttggc gttcatcata tttgggcaac ttctgatcgt cccttacctt
    18421 agaatctagt acttccttgc tgtctagtct catttggagc tttttcgctg tctttagccc
    18481 ccataccaac aatatgagaa agctagtcaa tataaccgct acgcctccaa ctaagtaacg
    18541 ttgaaaactg gttttagcca gcagtgtcgc tactaacaca accaaatcca tcactgctat
    18601 tatgattata aacatccatt gtgacttagc gagattttgt ttgctatcaa acatgattcg
    18661 tttctccttt aaatctttac atgacataat acaaattatt ttggatattt tgtcaaatat
    18721 attttacatt cttgattgat ttaagcagca aaaaaggatt gaggatttct cttcaatcct
    18781 tataagggat taacgggaat tctgcccaat cacaaaaact gccagggtgt taggaccaac
    18841 gtgagctgag ataactggtc ctagtggcat cataagaaca tcgctaatat tctcatgcgc
    18901 cagtagctct tctcgcaact tttcggcact accctgatcg cttgtataag agacaataac
    18961 cgtcgaatcc gcaatatctt tttccacttg agccaccatt tctttgatgg ctttttggcg
    19021 tccccgaatt tttgcaatgg gcaccaattt cccttcctca tcaatccaca aaagaggttt
    19081 gatactggct aaactgccta gaaaagctga accttttgac agacgcccac cacgcatcaa
    19141 atgaaaaaga tcatctacta agaaataggt gcgcagtcga gggatcactg cttcaacaat
    19201 atctttagtc tccagtaaat ttttgccact atctctggct tctgctgcta aaatggttag
    19261 atagccttct cctcctgcag cagccaaggt atcaacaatt tcaatcaccg catcgggata
    19321 atcttctcga acaagatcgc gtgccattag agcgctttga taggtacccg ataaaaccga
    19381 agaaaaagca agataaagca gtgctttgtt gttcctagca tgttcacgaa aaaccttctc
    19441 aaactctccc acattaatct gactggtttg aggatgactg ccagctttca tctttttcaa
    19501 aaggtaatca ctactaatac ggttaggacc gaccgtttca taaacctctc catcgcacaa
    19561 aatcgttaat cctataagga caatatcatg atcttctgcc caggtttgat tcaaatcagc
    19621 ggttgaatct gtcattattg taaaggtcat tcttacttct cctcatccag ttttttagtt
    19681 tgacaatcaa ataattattg ttttatttta tcaaaaaaac ccgcacttgg cgagtcgtaa
    19741 ttctcattat cgatctaact gagacataaa atccttcaat tgactaagct gtccttggtc
    19801 aaaaggccgc agtttaggcg ttttgagatc aatatctcta gacattttat ctaaatcagc
    19861 tttgacagta tgaactcttt gctctaattc tcttgcagtg acacgaagag ctccttgaga
    19921 aaaaatcgtc cattgttcat ttaaatcact cgtagctact tctaccatat gtatagccgt
    19981 atttaactct gctgccatgc gctcaatgta gctatctgct gtttcgtctt cctctgtaaa
    20041 taccactgag atataatact ggtcatatcg ttgccttagc cccggtacat attgggcatc
    20101 aaaaacacaa ataatattaa tattctcaaa atgggcataa tgattaagtt ttgttaaaag
    20161 tgtgttacgt gcctgatcaa gctgatttgt cttaaacaac tgacgggttg attgccaaaa
    20221 ggcaatcata ttatacccat ctactaataa tatccgtttt ttcataattt agcgcctatt
    20281 tcggaaaact tcatacatga gaatagctgc cgctacgcta gcatttaagc tctgtacgtg
    20341 accattcata ggaatcgtaa tcatttcatc aacttgcttt ttgatattag tagagatgcc
    20401 tttgccttcg ttaccaatga ctaaagcaag tttaccatta gtgttccagc aatcagacgg
    20461 tgttccattc atgtctgtgc caaaaatcca gaatcctctt gctttcaatt tatctagagt
    20521 ttgactaagg ttagttactc tcgctatcgg aatatgttca acagcacctg tagatgtctt
    20581 tgaaaccact ggagtcacac caacagaacg atgtttagga ataatcactc cacatacatt
    20641 tgtagcatca gctgtccgta aaatcgatcc taaattatga ggatctgtca gaccatctaa
    20701 aataagaatc aaaggatttg cttcttgctc tgctatttcg aggatctcat caacatcagt
    20761 ataggcaaaa gctgatactc tcagaacaaa accttgatgc acagctccat cagtcatttg
    20821 tgacaaggtt tttttaggcg tccatgaaat agcgaccttt ttttgtgtcg ctaagctttt
    20881 gatgttatcc actttctttc ctcttaaatc ctcttggata taaagcttat ttcctgtatt
    20941 tgcttgaagg ctttctgtaa cggcatgaac accatagacg atatcgtttg tttcaatagt
    21001 atctttatct tccatgagtt tattatacca tgaatgtttc aaaataaatg catagactct
    21061 tttattttcg tctagatact ttttgaatag ctataaataa acctgcagct aaactgacta
    21121 ctagacctga taagacccaa gggaatcctc tttttgaagt aggttttact tcctctttgt
    21181 ccactagcat ttttgtcttg ttgctaccgt ttttttggct attactagca gttggtgtaa
    21241 gtttctcatt tttagtagat cgctttaagg aagttgatcg atgattagta gctgatttga
    21301 aaggatttga agtagaatct atttcttggt tagctgattg ttttgtttga gcttttgtgc
    21361 ctacatcgac cgaattcgtc attgctttag aagtatcctt ttgttgcata ttagttggtg
    21421 tttttctagc tttgttttga tcaaaatgaa cagttcctga ttgaccagtt actgcctttt
    21481 cttgagtatg aacggtgaca gttacatgat agtcaccact cttttgtcca gttaaatcaa
    21541 accaagctgt aaaacgacgc tgttgcgctg ccatagtata tacccaacta ttaatagtat
    21601 tatcactagc gtctttaagt gttatcgttg cctgagttgg ttgtctgaca ggttgatctg
    21661 tggtgactgt agcatcaata taaggttctg ttccacgcat actggcttca acactgacct
    21721 gactaatagt tgattcagct ataacattat cagtgtgctg atcttgtgca tacacagata
    21781 tggctttgcc agaagtcccc agcaaaccaa taagtaaagc tagtgataat aagctaactg
    21841 ttttttgcat agttgtcata ataaatatct cctctttaaa ttagtcttac cctctcatta
    21901 taaactattc gaaaatgatt ccattatttt tatgtctttt ttgttacaaa tgtatccttt
    21961 ttatatcatt atgtcttttt gatgtcataa aaagacttaa tggctctcta cctttacttc
    22021 agatctatga aaataagagt ctcctagggg actcttatga ggatatcaat tgttgctttt
    22081 ctacatactc aatacaccat ctgatcaatt cttctaaccg ctctttttgg cccatcatat
    22141 caagataacc cataatggct tcaaaacctg tcgacatacg ataggtaata atatcggcat
    22201 tcttagcttt agtatggcta ttggtattgc gaccacgctt atagatgtct tcttcttttt
    22261 cggtcaatag ctgcgcttct aacatagcct gaatcaagtt ggcttgtgcc ttagcagaaa
    22321 catatctcgt tgctaaacgg tgtagctggc taggtttcgt tttaccttga aaaatgagat
    22381 gacgacgaac atagtaggaa taaaccgcat ccccttcaaa ggctagggca ataccattaa
    22441 tcaaattcac atcaactgga ttagtcacgc agccacctca caccatcttt ggtatctagc
    22501 agcttgatgc cttgagctgc taactgatct cgaatggcat cagctgtggc aaaatcacga
    22561 ttcgcgcgtg cttcttgtcg cttagcaatc aaggcttcaa tgtcaacctt aagcacctct
    22621 tcttcaaaga taatgccaaa aacagctaac attttctcaa aagcgctttt aacaggttct
    22681 gtataagagc cagaattaat ccacttggcc atgtcaaata cgacggtaat cccatttgct
    22741 gtattgaaat catcgtccat agcgtcttgg aaagctatta caaactgttt tagctcttgc
    22801 tcatctgcag tttctgtcag cggttgttgc aaggtgtttt taagatactt cagattgatt
    22861 tccgcatcat gaatagcctt ttcagtgaag ttaattggtt ttcggtactg ttgtgtcgca
    22921 agaaagaatc gcaaaacctg accatcaacg gtttgtaaca tgtcatgaac ggtcacaaaa
    22981 ttacctagtg acttggacat tttttcgtta tcaacagtga caaacccatt gtgcatccaa
    23041 tagttagcaa aagtcttgcc tgttttggcc tcagattggg caatttcatt ggtatgatga
    23101 ggaaattcca aatcagctcc gccaccatga atatcaatgg tatcaccaag aatttcagta
    23161 gccatcacag aacattcaat gtgccagcct ggacgaccaa acccccaggg gctatcccaa
    23221 gaaacctcac ctgccttagc tgatttccaa agagcaaagt ccagtggatt ttcttttaaa
    23281 gctgtctcag catctgttcg accactggct ccaacttcaa gttctgacag ggttttatta
    23341 gctagcttgg cataatgctc tgacttttcc acgcggaaat acacatctcc atctgcttcg
    23401 taagcaaaat ctttttcaat gaggctttca acaaatgaaa tgatttctgc tatataatcc
    23461 ataacacgag gattttgtgt ggctggctta acaccaagtg ccttggtatc ttctataaaa
    23521 gctgcaataa agcgatctga caattcttta ggagaaacac ctgcttgagt agcagccttg
    23581 ataattttat catcgacatc ggtaaaattg gaaatgtaat tgacctgata gccagtatac
    23641 tcaaaataac gtcgaatggt gtcaaaagcg accgctgatc tagcatttcc aatatgaata
    23701 taattataga ccgtcggtcc acaaacgtac atattgactg tattttcagt caaaggtaca
    23761 aacttgcgga gcgaacgggt catggtatca taaattttaa tcatgtaaac tccttattca
    23821 atagcctggc attagttgtc atcttgatat cctaagtctt tttctgaaat gctgttccac
    23881 cttttcaaca atttgatatt aataaccaaa cctattaacc aaaatagggt caaaatgaag
    23941 tatataatcg ctttaaccat gtcttgtctc agtaaaaaaa ttagactagc caccagtgta
    24001 atactaaaaa caaggataga caaactcata atgacataca gcccaactct tattttattc
    24061 atgaggctcc tttattaacg aaaaaccttc acaacctgtg aaggcttcag aaagtagaca
    24121 aacccacttt tatgctcaaa aagcatgaat agatctactg atttgacatc aggtttccta
    24181 tttctgcatg tggggtgagc caaaacagct actttttgtt gtggtaaaag tggcatcaat
    24241 attgacagtg atacccatac ggatttttga gatcttaggc tcaaaactaa ggtatggaat
    24301 tccaaaggaa gtcgctgccg taagcacaac ctgagaaaaa gtatcacaat agactgtttc
    24361 ttcagtctaa gacttgctta gttgatgctc ttttacgcgt tttttgtatt ttgtaacatt
    24421 cccatatcac tacactcagc aaccaaactg ataggactag ctaagttcaa cattactcta
    24481 ctagaaagtc caaaaatatt ttattggaag tcatcagtgc ttctttttgt tattttccga
    24541 tcattttgac aactgataag agacctctcg ttgtttttgt aaactttgaa tttgacgatt
    24601 atctttttgt ccatgcactc gtactatctt agctggcaca cctacaactg tcacgtcttc
    24661 aggaacatct gataacacaa cagctgctgc ccctacttta gcatttgctc cgatgtcaat
    24721 aggtccaatc acttgggcat gtgccgaaat taaggcacct tgtcgaatcg ttggatgacg
    24781 cttgccacaa tcttttccgg ttccacctag ggtcacccca tgataaagca tcacaccttt
    24841 ttcaacaatt gctgtctctc caataacaag accagcacca tggtcaatga agactccagg
    24901 agcgatttgt gctccaggat ggatttcaat ttgtgtccaa aatcgccaaa attggctatg
    24961 cattctggct agtaatttaa aatgatgtcg ccaaagaaaa tgagacaaac gatgagcagc
    25021 tagggctttg atgcctggat aagttaggat aacctcaaga ctattacgag cagctgggtc
    25081 tagagcttta acaatagcta tactttcttt ccaccaaccc atactaactc ctacgactta
    25141 tctaattctg accaatcaac aagaatttgt tggtcatcaa aaggattgat ggcgacaccg
    25201 aacgtttcat caagctcgtt ttcaccattt cttggttgat aaatatcatc aatcgtccaa
    25261 gtcagaatca ctccttcagc tttcctgaaa aggcctccaa aatcgtcttg gttgtaccat
    25321 ttcgcaaagc tttgaagatt tgaaaaggcg ggaacatagc tttttccttc aggcgttgac
    25381 atggtaggaa aaaggcggtc gtaatggtta tcatctttag gataaacaaa ggcgggaaca
    25441 aggtaaacct tttccatcgt atctgccgcc acattgtcat cactcataag cgtatttaac
    25501 acagtggtgt aatggttcat aaattgaatc atgtcactgc ttttgaaaat ggtagaattc
    25561 ccaaaatctc cctttttctt aaggttaaag acaagacctg ctgcaccaga tgtaatgact
    25621 tcttctagca ctgcaagagc tgaacgctcc agccaatact gactttgagc actcttttgt
    25681 tcctctttaa agaggaacat atcttcctta tcggtaaaaa caggggtaac cttgacacct
    25741 tctacctcta tgacgtaggg ttctttagca gcccagacag gaaaattatg gaaagcgttg
    25801 acaagggcaa cactatccaa aaaattatca ggcgcattga taaaagctct taagcgaata
    25861 tctaattcat ttgatttagt catgtttttc ttcttttttc tctggttttg gtgggcgtgg
    25921 aatcaaagct ttcattgagg catccacacg acccttctca tcaatcttaa tgaccttaac
    25981 atcaatgtct tcacccactt cgaggacatc tgacacgtta gctgttctcg tccaggcaat
    26041 ttcagaaata tgaacaagtg catctgtctt gtcaaagagg ttgacaaagg caccaaattt
    26101 ctcgatacgg ataaccttgg catggtaaac ttcgcctacc ttggcttcac gaacaagacc
    26161 tgcaataatt tctttagtgc ggtcaatggc agcttggtca cttgagtaga tagagacatt
    26221 tccttcgtcg tcaatatcaa ttttgacacc cgtttcggca ataatcttat caatagtttc
    26281 gccaccttta ccgataacca ctttgatttt gtctacgtca atcttgatgg tgtcaatttt
    26341 tggtgcagtt ggggctaact caggacgtgg ttcagcgata gttgcttcaa tcacatcaag
    26401 aatttcaaag cgagcttttt tagcttgtgc aagagcttct tctaggattt gaggggtaat
    26461 tcctgcaatc ttaatatcca tttgaagggc tgtaatccct tcacgagttc ctgccacttt
    26521 aaagtccata tctccaaagt ggtcttcaag accttggata tcggtcaaga ctgtataatt
    26581 cgtcccgtct gaaatgagac ccatggcaat ccctgccaca ggagccttga ttggtacacc
    26641 accagccatc agagcaaggg taccagcaca gatagaagcc tgagaagacg aaccgttaga
    26701 ttctagaact tcggcaacta aacgaatggc ataaggaaat tcttctaagc ttggcaagac
    26761 ttgttcaaga gcgcgttcac ctaaggcacc gtgtccaatt tcacgacgac cagcagctcc
    26821 gtaacgaccc gtttcaccaa ctgaatattg cgggaaattg tagtggtgta aaaagcgttt
    26881 tttgtactct ggagccagac catcaatgat ttgagtttct cccattggtg ccaaggtcaa
    26941 aactgatagc gcctgagttt gaccacgtgt aaagagacct gatccatgta cttttggcaa
    27001 gaagtcaacc acagcatcaa gcggacgaat ttcgtcaatc ttacgaccgt caggacgaat
    27061 cttgtcctct gtaatgaggc ggcgcacttc agcatgctcc atttgttcaa ggatttctgc
    27121 cacgtcacgc ataatggttg ctaggttttc gtcttctgcg taacgctctt cgtactcagc
    27181 cttaaccatt tctttaacgg cttctgtggc tgcttcacgc gcttttttct cttcaacttg
    27241 aacagctttt tgcagttgag cattgtactt agccacaata tcagcttgta agtctgcatc
    27301 aacctgtaac agttcaacct cagctttctc tttaccaaca actgctacga tttgttcttg
    27361 gaaggcaatt aattcttgaa tggcttggtg accttttaaa agagcctcaa gcatgatgtc
    27421 ttctgataat tctttggcac cagattcaac catgttgatg gcttctttgc taccagcaac
    27481 ggttaattct aagagcgacg cttccatttg ttctttatct gggttgatga tgaactcgcc
    27541 atcaatatag ccaacttgaa cacctgcaat cggtccatta aatggaatat ctgagatgga
    27601 caaggccaga gatgagccaa acatcgctgc cattggagcg ctagcatttt catcataaga
    27661 aaggacagtg ttaatcactt gtacttcgtt acggaaacct tcggcaaaca tgggacgaat
    27721 cgggcggtca atcaagcgag ctgtcaaggt cgcatctgtt gaagggcgtc cctcacgttt
    27781 catgaaacct cctgggaatt taccagcggc atacattttt tcttcgtaat taacttgaag
    27841 agggaagaaa tcacccgttg ccattttctt agacatgaca gctgcggtta ggacagttga
    27901 gtcgccataa cgaacaacgg ttgccccatt ggcttgctta gcgacttgac caacttcaac
    27961 aacaaggggt ttccctgcaa atgttgttgt aaaggtttgt tttgacatat attctccttt
    28021 taattttcag aggggtaaag aggtacctgt tctctgctta gattcagttg ttatacgttg
    28081 ttttgctaca aaaagcaacc tgccacgctt gtcacaagct cctctttgta tcaaacgacg
    28141 tatctgctac attttaccat aaaatagcag aaatttcact gtcagatcgc aatctcttga
    28201 aatgtccttg aaaaatatta aacgaaactt atactcttta acaaagcaaa aaaagcaggt
    28261 tggtatgcct gctttcgtct cctaatatta atgtttggca tcctctaaaa ttttaaagcc
    28321 gaggctggta ctgtctttca gaaacatttg cacggtttca agggaatagt cttctaaatc
    28381 ttttccttga agaggcaact tggcaatcaa atcagtggta caagtgataa tgtccacgcc
    28441 tagctgatca gcttgataga tattataagt ttcacgagga ctagcccaca acaattcgac
    28501 cccttctttt tgacgacaaa tgcgtagagc ttcttccatg attggcattg ggtcaacgcc
    28561 ggtatctgca atacgaccag caaaaacaga cacaatagct ggaactcgag cggttaaatg
    28621 atcaaccaca gcttgagtct gctcaattgt aaaaatggct gtgacgttta acttaatgcc
    28681 tttggctgac aacttttgaa tgaggggaca ggttgattct cctgttgaag tggtgactgg
    28741 aattttcaca tagacattgt cacctagact ggcaattttc tcagcttcct tttccatgct
    28801 tgcgagatca tccgcaaaga cttcaaagga cactgggtag tctgtaattt tggcaagcac
    28861 ttctttggca aagccaatat aagaactgat gcctgctttt ttcatcaaac ttggattggt
    28921 tgtaaagcct gtgaccaaac ctgtttgtaa atcctttaac atggtctcta agaccgcacc
    28981 gtctgaaaag actttaatgt ttagtttttt tgtcatcata tcctcctttt agatttcttc
    29041 tgaaacatta ttgatggcct cttgagattt tgacagaaag cttggaagca ccaatacaat
    29101 agtaagcaca gcaaccacca agtaaatacc gatttgacca atcgacgttc ctaagcggcc
    29161 cagtaaaatc cctaaaacac cgaaatccac atctccaaag gtcgtgtttg aaaaaccaag
    29221 tttaccaagt acaggtagaa gcatggctgg caagaaggcg agtaataagc cattagcgaa
    29281 agcaccaatc atcgcaccgc gacgaccccc tgttgaattt ccaaaaatcc ctgctgttgc
    29341 accacagaag aaatgaggca ccatacctgg gataatcagg acaccacctg caactcctaa
    29401 aatcaacatc ccaagtaagc caccaacgaa acttgacgca aaaccgatga taacagcggt
    29461 tggcgcataa gggaagaata ccgcacagtc tacagctgga atagcatttg gaattaattt
    29521 gttagcaata ccttggaagg ctggaatcaa gtcagccaaa atcatgcgaa caccggcgta
    29581 gacaattgcc acaccaaccg caaaggtaag accactctta atagcaaaga taaatgggtt
    29641 ttgacctgct gctaattctt ctgttactga agcattcctc aaaacagaag ccactgttgc
    29701 caccaagtag aaaatcacca tgataagtcc tgttgaaatg gtggtgttgc gtaagaaact
    29761 ccatttttca gaaatctgaa ggtcctcggt atctttactg tctttgccaa ccttgctacc
    29821 aacccaggca gaaaggtaat agcccaaact accaaagtgt cccatggcga tttcatctcc
    29881 gtcagtcact ttcagagtat actgttgacc aatggctggc gaaatagctg accaagctcc
    29941 caagaggaac ccacctaaga taatcaaaag acttcctttg aaaccaacag ctccaagcac
    30001 ggcggacaag agacaagcca taaagaagct gtgatgtcct gttaagaaaa tgtatttgaa
    30061 gcgggtaaaa cgagcaaaag ccaaattgag caataaccca accaccaaga tggacatggt
    30121 ttccacaccc agaatctttt gagcgacaga agtgacggct tcattatttg gcactacgcc
    30181 agtgatgcta aaaccgtgct caatgagttt ggcaagaggg tccagattgg tcacaataac
    30241 acccgcccct gcagcaagca taaggtatcc caaaatcggc cccaaggttc ctgtcaacac
    30301 cttatgagca ggtgttttca aagccaccaa ccctgcaaag gcaatcaagc ccattaaaaa
    30361 tgcaggttct tttaaaatat ctcgaataaa tgataataat gcttccatag tatcttccta
    30421 tctcctttaa aggcttattc cttgccactc atttccttga gaaccggaac caaatcttct
    30481 tcaatttttt tacgattggt atagctttta acgatacaaa ccttggcatc ctctggaaaa
    30541 atatcttcaa attctttaac ggtaacaaac aaatctgcac cctttccaac agccgcattg
    30601 gaatcacagg attctgcatc cacatcaata cccaaactag aggcgatagc ttctactttc
    30661 atgcgaagta acaagctact accaataccg tttccacaaa ccgttacaat tttaatcatg
    30721 tgattcatct cctttttcaa tcatatggct aatcagactc attatttcct cagtattctt
    30781 agcctcaatt aattgctcaa tatgttcatc atcatctaaa atcaacgaca gttcctgcaa
    30841 agccttcaaa tgtgaactag aatccacagc agacaaaaca aaaatcaatt gcacttgctt
    30901 gtctggatca ccttcttgct tcaaatcaaa gctgacaggt tcttttagtt gtaagagaga
    30961 catgcctaac tgccgcgttc ctttttcagg cgccgcatga ggaacagcca ccttaggggc
    31021 taaaacaata taggccccaa gctcattgac cgaatcaatc atgccatcga tataagatgt
    31081 ttcaatcatc tggtgttcta gtaaaggctg agcagccaaa cgaatcgctt cctgccaatc
    31141 cgaaacagca tcagcctgcc taataaaatc tgctgttaac aagtccaata gtcctagacc
    31201 tcctttttca atcgttaaat ggttaccaat caggtaacgg gataaatcac taacaagtcc
    31261 ttctttatta gtaatcgtgg tgtgtttatg aataatactc aacaggtcat ccaaagcgaa
    31321 ttgctctgac aagggaagat gaaaatcatc acagaccatc tttttcaaca tcattttctc
    31381 aacaggtccc atcaaagctt gcgtcacata aacaggttta gcacagtcaa aagctactgt
    31441 tgaaaaaatc aaatcaaagg aagctggatc caacaacttt attttgtcca gctggtgaat
    31501 cctaataaac tgtagctgtg gaaaaagttc cttcaaggtt gcctccaaca tcagagacga
    31561 actaatgcca ttagggcaaa cagataaggc taccagctga ttgcttggcc tcttcttagg
    31621 ggcctgcaac catctcccaa aatgaatggt aaaataagca acctcatctt cattaactga
    31681 tttccccaat tgtttttcta gaggagaaag gctgcgcttg accaagtaaa agagagattc
    31741 ataatcttgt ttgatttgtt ccttcaaagg attggtcaag ttaatgtcgt agtaaagtcg
    31801 gtaataagct ggcaaaaggt gactgtatag gttctgacga agttctgggg tatcggtgat
    31861 agacaatccc gtgttaaccg ccaccgaatt gatgatttcc tccataatgt cataaatagg
    31921 ttgctgaaag actaactcta agtcaccttg gatacaacca agtaacctag attgaaccaa
    31981 atatttttct tcggttagac tggggaagtt gaccaataac tggtccacca tcgtttcaac
    32041 agctggcgaa gctaaaggaa aagtatccac tgcttcagct cttacattcc gtgaaaatgg
    32101 tcgatgagcg agaaggcaga aaaaataaat caaatccctc aatttctccg aaataaaagt
    32161 taagtggttc tccctgctca gttcctccaa cgttgctgcc atcacctttg tctggtcggg
    32221 caaaccaagt tcatgcaata agtaactgaa cacccaagat cctgatatga cttgcaaaag
    32281 agaactgacc gctgattcta agagacgccg caaggctaag ggttctcctt caaaaaaata
    32341 accatcctgg cgattgtaag ctaaagttac ctgaaaggct tgtacccgat cacgcaccaa
    32401 gtttaaatca gcaataacag aatttctgct taggcgtaac aagctttcca agtggttaat
    32461 ggagataaat tctttagcca tcatgatgta aatgataatc atgtccagac gctcatcttg
    32521 aaagagatag tcgtctaagg taggtagcat gcgattcaac aagtcctttt cagtaaggtc
    32581 tggcggataa accagctgcc tgtccagagc aataaggggt aactgataga gtgacaacgt
    32641 gtcattgatt ttagcaatat ctgccagtat atgacgtttg cttgtcttta atatggattc
    32701 aaatgcctcc agtgaatgtc ctttatattt agaaaagagt tgatagtttc tgatgagctc
    32761 gtgagatagc atgtcatctc ctcctataat gtcagtataa aatgaagcgt tttcattttc
    32821 aagacaaatt gagtccaatt gtctctcgtc atttttaact gcaattggac tcaacaggtg
    32881 atatcattgt aacaagttcc tcaaaaggtg gcaaaaaata ggcaaatctg ggaattgatt
    32941 ccctttttct tcatgataca acaaaagata cctcatcact gagatacctt ttgttgctaa
    33001 ataaaatcat caactgctaa aaaaacagtc ttagaccatt ctagccaaat gtgtaagtta
    33061 acaaacaaca tgtcaaaaat gaaaaagccc actgcataag cagaggactt tttaaacttg
    33121 aaattaacga cgaagtccaa gagattggat caactcacgg taacggttaa catctgtacg
    33181 acgtaggtat gccaacaagt tacgacggtg accaattttt ttcatcaatc cacggtaagt
    33241 agcgtggtct tttttgtgtt ctttgatgtg gctgttcaag tggttgattt cccatgtaag
    33301 aactgctact tgaacttcaa ctgaacctgt atcgccttcg tgacgtgcat attgagcaat
    33361 gatttcattt tttttctctt ttgagattgc cataatatat tctccttttt ttgcttaatc
    33421 cgagtcctag gattggcact cctaaaacca agaaaaagtt ggtttgtctt tcgaccactc
    33481 tattctagca gattcataat attaggtcaa ccgttatcct attattctga gctctcacat
    33541 ccaaaccacc tcactaggct taaaaaggtt atctggtcat tgaatgtgct ctttttgctt
    33601 taaccaaacc accataagca acagaactag caaggccaaa tagccaaaag gctgttttta
    33661 aatcagaata aacctgtgcg attgcttgcc taataagata accagctaaa agaggtaaga
    33721 tggctgatcc cagcacttca gctgataagc ggtagcctag catagtcatt catttctttc
    33781 caataaagtg ttcactgata atatgaattg ctctagtatt aacaaaccca atccctaatc
    33841 catgaacaca gcttattaac cgatgctacc tgcctttatg acataggtca agagttgaca
    33901 caaaatgttt gccaacaaaa cattacctat atcgtcaaag agcgctttgc ttcctatatc
    33961 cttaaagcgc aagccaacca agaggttcat ctgaacttta tattattagc aaaccgtttt
    34021 gaaacaagtg atcgccatct taagcacgta cttaagcagc ccatttttca gaggattatc
    34081 gaacgcatct gcttcaaaac ctatcgtatt gccgatttag acgctctaac tcaattaaga
    34141 cctctgaggt atgcagtcca ttaacttata ataacaatca ctctaaccac tatctgactt
    34201 ttctttaaaa aagaaatgat ttgtgatata gtaaatccag ttaaaaaaaa gtaaaggagt
    34261 agacatgtct gcacaagata aattaattaa acctagtcat ctgattacca tggatgacat
    34321 tattcgtgaa gggaatccaa ctttaagagc tgtcgccaaa gaagtgagct tacccttgtg
    34381 tgatgaggat atcctattag gagaaaagat gatgcaattt ttgaagcatt ctcaagatcc
    34441 tgtaatggcg gaaaaacttg gtcttagggc cggtgttggt ctagcagctc ctcagattga
    34501 tgtgtcaaaa cgtatcatcg ctgtgctcgt tcctaatctt cctgataaag aaggaaatcc
    34561 tcctaaagaa gcctatagct ggcaagaagt cctgtataat cctaaaatcg tttctcattc
    34621 tgttcaagat gcagcccttt ctgatggtga aggttgttta tctgtcgatc gtgttgttga
    34681 agggtatgtg gttcgccatg ctcgtgttac agtagactac tatgataaag aaggacaaca
    34741 gcatcgcatc aaattaaagg gatacaacgc tattgtggtt caacatgaaa ttgaccatat
    34801 caatggtgtt ttgttctacg atcggatcaa tgctaaaaac ccctttgaaa ccaaagaa
//
LOCUS       DAUCLR010000010        34746 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.010, whole genome shotgun sequence.
ACCESSION   DAUCLR010000010 DAUCLR010000000
VERSION     DAUCLR010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34746
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.010"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(65..1018)
                     /gene="manA"
                     /locus_tag="VGT87_000373"
     CDS             complement(65..1018)
                     /gene="manA"
                     /locus_tag="VGT87_000373"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEQ3813261.1"
                     /translation="MSEPLFLKSTMHDRIWGGTKLRDVFAYNIPSDTTGEYWAISAHP
                     NGVSTVTNGRYQGQPLNTLYAQEPALFGNPKEEVFPLLTKILDANDWLSVQVHPDDAY
                     GHEHEGELGKTECWYIISAEEGSEIVYGHQAKSKEDLRAMIEAGAWDDLLTRVPVKAG
                     DFFYVPSGTMHAIGKGILILETQQSSDTTYRVYDFDRKDVNGNLRDLHIEKSIDVLTI
                     GKPENSVPATMVLDNMVATTLVSTPFFTVYKWVTSQMVDMKQAAPYLLVSVLKGQGKL
                     YVDQKAYELEKGMHFILPNDVKSWSFDGQLEMIVSHPNQLV"
     gene            complement(1113..1997)
                     /locus_tag="VGT87_000374"
     CDS             complement(1113..1997)
                     /locus_tag="VGT87_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEQ3813262.1"
                     /translation="MGKLYGSIEAGGTKFVCAVGDEEFTVVDKTQFPTTTPEETIAQT
                     IAYFKTFEADLAGMAIGSFGPIDIDPSSETYGYITTTPKSGWANVDLLGQLSAAFKIP
                     FDVTTDVNSSAYGEVLARPGVESLVYYTIGTGIGAGAIQHGHFIGGLGHTEAGHTYVM
                     LHPDDMAKGFLGVCPFHKGCLEGMAAGPSIEARTGVRGERLDQEADVWDIQAFYIAQA
                     ALQATMLYRPQAIVFGGGVMAQEHMVLRVHDKFTTLLSGYLPVPDLTDYIVTPAVVDN
                     GSATLGNFALAKLAAQGK"
     gene            complement(2261..5239)
                     /locus_tag="VGT87_000375"
     CDS             complement(2261..5239)
                     /locus_tag="VGT87_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HEQ3813263.1"
                     /translation="MVYQLGSHDLVPKIRSVQMDKHLLVKRTLGCVCAATLMGAALAT
                     HHDSLNTVKAEEKTVQVQKELSSIDSLHYLSENSKKEFKEELSKEKVPEKVKEILAKA
                     QQADKQAQELTKMKIPEKIPMKPLHGPLYGGYFRTWHDKTSDPTEKDKVNSMGELPKE
                     VDLAFIFHDWTKDYSLFWKELATKHVPKLNKQGTRVIRTIPWRFLTGGDNSGIAEDTS
                     KYPNTPEGNKALAKAIVDEYVYKYNLDGLDVDVEHDSIPKVDKKEDTAGVERSIQVFE
                     EIGKLIGPKGADKSRLFIMDSTYMADKNPLIERGAPYINLLLVQVYGSQGEKGGWEPV
                     SNRPEKTMEERWQGYSKYIRPEQYMIGFSFYEERAGSGNLWYDINVEDESNPNIGKEI
                     KGTRAERYAKWQPKTGGVKGGIFSYAVDRDGVAHPKKNGYKNPKLDNIVTSDYSVSKA
                     LKTVMLKDKSYDLIDEKDFPDKALREAVMAQVGTRKGDLERFNGTLRLDNPAIQSLEG
                     LNKFKKLAQLDLIGLSRITKLDRSVLPANMKPGKDTLETVLETYKKDNKEEPATIPPV
                     SLKVSGLTGLKELDLSGFDRETLAGLDAATLTSLEKVDISGNKLDLAPGTENRQIFDT
                     MLSTVSNHAGSNEQTVKFDKQKPTGHYPDTYGKTSLRLPVAEGNIDLQSQLLFGTVTN
                     QGTLINSEADYKAYQNHKIAGRSFVDSNYHYNNFKVSYENYTVKVTDSTLGTTTDKTL
                     ATDKEETYKVDFFSPADKTKAVHTAKVIVGDEKTMMVNLAAGATVIKSENDENAKKVF
                     NGIMEYNPLSFNNKSSIIFEMKDPSLAKYWRLFNDSSKGKDDYIKEAKLEVFTGQLNA
                     EADVKTSLEKSDDWQTVSTYSGQEQVFSHALDNISAKYWRITVDNKKNQYGCVSLPEL
                     QILGYPLPNADTIMKTVTAAKELSQQKDKFSQKMLDELKIKEMALETSLNSKIFDVTA
                     INANAGVLKDCIEKRQLLKK"
     gene            complement(5416..7299)
                     /locus_tag="VGT87_000376"
     CDS             complement(5416..7299)
                     /locus_tag="VGT87_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEQ3813264.1"
                     /translation="MDNRQIATEVIEALGGRENVRSVAHCATRLRVMVHDEGKIDKEK
                     AEAIDKVKGAFFNSGQYQMIFGTGTVNNIYGEVVALGLPTSSTSEQKAEAGKQGNIFQ
                     RAIRTFGDVFVPIIPAIVATGLFMGVRGLVTQPAIMDLFGVHEYGENFLMYTRILTDT
                     AFVYLPALVAWSAFRVFGGNPIIGIVLGLMLVSNELPNAWVVASGGDVKPLTFFGFVP
                     VVGYQGTVLPAFFVGLVGAKLEKWLHKKVPEALDLLVTPFLTFAIMSILGLFVIGPVF
                     HSLENLVLAGTQAVLHLPFGIAGLIVGGIQQLIVVTGIHHIFNFLEAQLIANTGKDPF
                     NAYLTAATAAQAGATLAVAVKTKSPKLKGLAFPSTLSALLGITEPAIFGVNLRYPKVF
                     VSGLIGGALGGWVAGLFGIAGTGFGITVLPGTLLYLNGQLLQYLVTMLVGLGLAFAIA
                     YTWGYQDRETLPLPAVEVDQTADQPALAEETLYSPLNGTVVDLAAVSDPVFSSGAMGQ
                     GLAIKPEDNTLYSPVDGKVEIVFETGHAYAITSSQGAEVLLHIGIDTVSMAGDGFESL
                     VAVGQAVKKGDLLGHFDPSKIAEAGLDDTTMMIVTNSADYQSVDILAQGHVLIGDQVA
                     LIK"
     gene            7541..8980
                     /locus_tag="VGT87_000377"
     CDS             7541..8980
                     /locus_tag="VGT87_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEQ3813265.1"
                     /translation="MDLPQAIRYRPYKEWSSKDYQAITEKMAQSPWHSQFHVEPKTGL
                     LNDPNGFSYFNGRYHLFYQNWPYGAAHGLKQWVHMTSTDLVHFTETRSRLLPDHAHDS
                     HGAYSGSAYAIDDKLFLFYTGNVRNANWVRTPLQVGAWMDKQGNISKIPQVLIEQPDD
                     VTEHFRDPQLFSYQGQFYAIIGAQGLDGKGKIKLYKAVDNHVDNWRFIADLDFDDSGT
                     EYMIECPNLVFVDDKPVLIFSPQGLAKADLDYQNIYPNTYKIFESFNPETGQLLGGGA
                     LQNLDFGFEAYATQAFSSPDGRVLAVSWIGLPDIDYPTDRYNYQGALSLVKELRIKDG
                     ILYQTPVSALQNLRGPAELFHNKIDSSNCYELELTIPGQKKLDLLLFADQKGNGLRLK
                     VDTTKGQLSIDRSRAGVQYAQDYGTVRSCQIPQGHVTLNVYVDNSILEIFINQGQKVL
                     TSRVFPTQGQTGIQVVEGQAFGHYYEMRY"
     gene            8985..9950
                     /locus_tag="VGT87_000378"
     CDS             8985..9950
                     /locus_tag="VGT87_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEQ3813266.1"
                     /translation="MVAKLTDVAALAGVSPTTVSRVINKKGYLSQKTVNKVNKAMREL
                     GYKPNNLARSLQGKSTQLIGLIFPNISNIFYAELIEHLEIELFKQGYKTIICNSEHNP
                     VKEREYLEMLAANQVDGIISSSHNLGIEDYERVEAPIVAFDRNLAPNIPVISSDNFEG
                     GKLAAQTLQKHGCQNIVMITGNDNSDSPTGLRQLGFNYQLKRSAEILKLPNNLSPVRR
                     EMEIKSILATRKPDGLFVSDDLTAILIMKVAKQLHITIPEDMKVIGYDGTTFIQQYVP
                     QLATIRQPIDEIAKLSVEILIKKIKKEKTSKDYILPITLLPGASI"
     gene            complement(10091..10543)
                     /gene="nusB"
                     /locus_tag="VGT87_000379"
     CDS             complement(10091..10543)
                     /gene="nusB"
                     /locus_tag="VGT87_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEQ3813267.1"
                     /translation="MTNSFQNSRRDLRERAFQALFNIEMGAELLAASQFAYGYDKVTG
                     EDAQVLELPIFLLSLVTGVNNHKEELDNLISTHLKKGWSLERLTLTDKTLLRLGLFEI
                     KYFDETPDRVALNEIIEVAKKYSDETSAKFINGLLSQYVSEAPSANKS"
     gene            complement(10536..10925)
                     /locus_tag="VGT87_000380"
     CDS             complement(10536..10925)
                     /locus_tag="VGT87_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEQ3813268.1"
                     /translation="MTTEYIGEIVISPRVLEVITGIATTQVEGVHSLHNKKMADSFNK
                     ASLGKGVYLQTEEDGSVTADIYVYLQYGVKVPTVSMNIQKTVKSAVYDMAEVPISAVN
                     IHVEGIVAEKTPKPDLKSLFDEDFLDD"
     gene            complement(10971..11528)
                     /gene="efp"
                     /locus_tag="VGT87_000381"
     CDS             complement(10971..11528)
                     /gene="efp"
                     /locus_tag="VGT87_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEQ3813269.1"
                     /translation="MIEASKLKAGMTFEAEGKLIRVLEASHHKPGKGNTIMRMKLRDV
                     RTGSTFDTTYRPDEKFEQAIIETVPAQYLYKMDDTAYFMNTDTYDQYEIPVANVEQEL
                     LYILENSDVKIQFYGSEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATLETGLVV
                     NVPDFIEAGQKLIINTAEGTYVSRA"
     gene            complement(11624..12085)
                     /locus_tag="VGT87_000382"
     CDS             complement(11624..12085)
                     /locus_tag="VGT87_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComE"
                     /protein_id="HEQ3813270.1"
                     /translation="MTNRLSWQDYFMANAELISKRSTCDRAFVGAVLVKDNRIIATGY
                     NGGVSATDNCNEAGHYMEDGHCIRTVHAEMNALIQCAKEGISTDGTEIYVTHFPCINC
                     TKALLQAGITKITYKAHYRPHPFAIELMEKKGVAYVQHDVPQIVLGGDKEQ"
     gene            complement(12120..13193)
                     /locus_tag="VGT87_000383"
     CDS             complement(12120..13193)
                     /locus_tag="VGT87_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEQ3813271.1"
                     /translation="MSGFLEQRLGHCLRQMAEKGLEALLVTHLTNSYYLTGFSGTAAT
                     VLITAKRRVLITDSRYTLLAKASVEGFDIIESRTPLKVVAELLGADQIDCLGFEDQVS
                     FSFYQAMQAELSGITLLAQSGFVEHLRLIKDVSEIETIAKACSISDKAFEDALDFIKP
                     GTTTERDLANFLDFRMRQYGASGTSFDIIAASGYRSAMPHGRASDKVIQNKESLTMDF
                     GCYYNHYVSDMTRTIHIGQVTDEEREIYALVLAANKALIAKASAGMTYSDFDGIPRQL
                     ITEAGYGSHFTHGIGHGIGLDIHENPFLGKSEQLLQAGMVVTDEPGIYLDNKYGVRIE
                     DDLVITKTGCQVLTLAPKELIVL"
     gene            complement(13309..16137)
                     /gene="uvrA"
                     /locus_tag="VGT87_000384"
     CDS             complement(13309..16137)
                     /gene="uvrA"
                     /locus_tag="VGT87_000384"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEQ3813272.1"
                     /translation="MQNKIIIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGNMEKPDVDSIDGLSPAISIDQKTTSKNPRSTVGTV
                     TEINDYLRLLYARVGTPYCINGHGAITASSAEQIVEQVLALPERTRMQILAPVVRRKK
                     GQHKTVFEKIQKDGYVRVRVDGDIFDVTGVPELSKSKMHNIEVVIDRLVNKDGIRSRL
                     FDSVEAALRLGDGYLMIDTMDGNELLFSEHYSCPVCGFTVPELEPRLFSFNAPFGSCP
                     TCDGLGIKLEVDLDLVVPDPSKSLKEGALAPWNPISSNYYPTMLEQAMASFGVDMDTP
                     FEALTEEERDLVLYGSGDREFHFHYVNDFGGERNIDIPFEGVVTNVNRRYHETNSDYT
                     RNVMRGYMNELTCATCHGYRLNDQALCVHVGGEEGPHIGQISELSIADHLQLLEELEL
                     TENESTIAKPIVKEIHDRLTFLNNVGLNYLTLSRAAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIESLKKMRDLGNTLIVVEHDEDTMMQADWLIDVGPGA
                     GEFGGEIIASGTPKQVAKNKKSITGQYLSGKKFIPVPLERRSGNGRFIEIKGAAQNNL
                     QSLDVRFPLGKFIAVTGVSGSGKSTLVNSILKKAVAQKLNRNADKPGKYHSISGIEHI
                     ERLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVPCEVCHGRRYNSETLEVHYKEKNIAEVLDMTVDDALV
                     FFSAIPKIARKIQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSLYIL
                     DEPTTGLHTDDIARLLKVLERFVDDGNTVLVIEHNLDVIKSADHIIDLGPEGGVGGGQ
                     IVATGTPEEVAQVKESYTGHYLKVKLQQ"
     gene            16340..17284
                     /locus_tag="VGT87_000385"
     CDS             16340..17284
                     /locus_tag="VGT87_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEQ3813273.1"
                     /translation="MKQMFLSSAIEFKEIETFEPGAWIKLVNPSQEESMKIADQFNID
                     ISDLRAPLDVEETSRIAVEDDYTLIIVDVPIYEERNNKSYYITMPLGIIVTENAVITT
                     CLHDMTLFDHFHNRRVKNFYTFMKTRFVFQILYRNAELFLTALRTIDRQSERLEAQLE
                     AATRNEELIDMMELEKSIVYLKASLKFNERIVKKLSSSTSSLKKYIEDEDLLEDTLIE
                     TQQAIEMAGIYENVLNAMTETTASIINNNQNTIMKTLALMTMALDIPTVIFSAYGMNF
                     QNNWLPLNGLEHAFWYITLIAMLLSSFVVIYFIRKKWF"
     gene            17417..18073
                     /locus_tag="VGT87_000386"
     CDS             17417..18073
                     /locus_tag="VGT87_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEQ3813274.1"
                     /translation="MELQELTKKNQEFIHTATNKLIQDGKSDEDIKLILEEAIPAILE
                     NQKKGVTARNLLGTPTAWAASFSQDPSQRAAETDKNTNPWLMWLDTSLLFIGIVALLN
                     GIMTFFNTNATVTGLISLLALGFGGGASMYATYYFIYRHLGKDKSLRPSWFKIIAALS
                     LAMLIWIALYSATAFLPTSLNPQLPPLALLIIGGVSLALRYYLQRKYNIQNTMSPVNK
                     "
     gene            complement(18206..18445)
                     /locus_tag="VGT87_000387"
     CDS             complement(18206..18445)
                     /locus_tag="VGT87_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEQ3813275.1"
                     /translation="MAQQRRGGFKRRKKVDFIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPYVNED"
     gene            complement(18610..19101)
                     /locus_tag="VGT87_000388"
     CDS             complement(18610..19101)
                     /locus_tag="VGT87_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEQ3813276.1"
                     /translation="MINNVVLVGRMTKDAELRYTPSQVAVATFTLAVNRTFKSQNGER
                     EADFINCVIWRQPAENLANWAKKGALIGVTGRIQTRNYENQQGQRVYVTEVVADNFQM
                     LESRATREGGSTGSFNGGFNNNTSSSNSYSAPAQQTPNFGRDDSPFGNSNPMDISDDD
                     LPF"
     gene            complement(19123..19413)
                     /locus_tag="VGT87_000389"
     CDS             complement(19123..19413)
                     /locus_tag="VGT87_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEQ3813277.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKDWE
                     KRRLAYEINDFREGLYHIVNLEATDAAALNEFDRLSKINGDILRHMIVKLDA"
     gene            complement(19586..19879)
                     /locus_tag="VGT87_000390"
     CDS             complement(19586..19879)
                     /locus_tag="VGT87_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813278.1"
                     /translation="MFQKLKNLIDRYSWFVFIMMILVLFLNYIYPFDPSLSGLQLEWL
                     IMTQVFPGLGIVCAMVAIIFKKFNLFVLALILYFALWLKLFLAFSFFTYFLGV"
     gene            20077..21201
                     /gene="mutY"
                     /locus_tag="VGT87_000391"
     CDS             20077..21201
                     /gene="mutY"
                     /locus_tag="VGT87_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEQ3813279.1"
                     /translation="MWDNETIASFRRTLLGWYDQEKRDLPWRRTTNPYYIWVSEIMLQ
                     QTQVNTVIPYYKRFLEWFPQIKDLADAPEEQLLKAWEGLGYYSRVRNMQKAAQQVMVD
                     FGGIFPHTYDDIASLKGIGPYTAGAIASISFNLPEPAVDGNVMRVMARLFEVNYDIGD
                     PKNRKIFQAIMEILIDPDRPGDFNQALMDLGTDIESAKTPRPDESPIRFFNAAYLNGT
                     YGKYPIKNPKKKPKPMRIQAFVIRNQNGQYLLEKNTKGRLLGGFWSFPIIETSPLSQQ
                     LDLFDDNQSNPIIWQTQNETFEREYQLKPQWTDNHFPNIKHTFSHQKWTIELIEGVVK
                     ATDLPNAPHLKWVAIEDFSLYPFATPQKKMLETYLKQKNT"
     gene            21378..21965
                     /locus_tag="VGT87_000392"
     CDS             21378..21965
                     /locus_tag="VGT87_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ3813280.1"
                     /translation="MEIGQQIIRYRKQQALSQEELAEKVYVSRQSISNWENDKTYPDI
                     HSLLLLSQIFQVSLDQLIKRDIEKMKYTITQVDKKNFKRDTKVMVTLMILLMISSYPL
                     VYFLEWLGLGIFVLLSIITMTYANRVERFKKKYDVQPYKEILAVSSGKLLDEIEKREE
                     RAKLPYQNPLIVTVFFLITVAIAFASRFMFTWLFH"
     gene            complement(22017..22331)
                     /gene="trxA"
                     /locus_tag="VGT87_000393"
     CDS             complement(22017..22331)
                     /gene="trxA"
                     /locus_tag="VGT87_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEQ3813281.1"
                     /translation="MALEVTDATFVEETKEGLVLIDFWATWCGPCRMQTPILEQLSQE
                     IDEDELKILKMDVDENPETARQFGIMSIPTLMFKKDGEVVKQVAGVHTKDQLKAIIAE
                     LS"
     gene            complement(22412..22912)
                     /locus_tag="VGT87_000394"
     CDS             complement(22412..22912)
                     /locus_tag="VGT87_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEQ3813282.1"
                     /translation="MESYEHFYAKLSQPFRKSPQLIILLNVLLKIITGVMYILYPSFL
                     IFTLWQGMTFQLWLRLLIIPAVGFIALSYIRKRFDFPRPYEKWNIKPLIDKDTKGRSM
                     PSRHVFSATMISMCLLRYYVYFGIVCLILSVLLAICRVIAGIHYPKDVIVGYLIGLML
                     GLCLLI"
     gene            complement(22916..25255)
                     /locus_tag="VGT87_000395"
     CDS             complement(22916..25255)
                     /locus_tag="VGT87_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEQ3813283.1"
                     /translation="MNNKILEQLEFNKVKELLLPYLKTEQSQEELLELEPMTEAPKIE
                     KSFNEISDMEQIFVEHHSFGIVSLSSISESLKRLELSADLNIQELLAIKKVLQSSSDM
                     IHFYSDLDNVSFQSLDRLFENLEQFPNLQGSFQAINDGGFLEHFASPELERIRRQLTN
                     SERRVRQILQDMLKEKAELLSENLIASRSGRSVLPVKNTYRNRISGVVHDISSSGSTV
                     YIEPRAVVTLNEEITQLRADERHEEGRILHAFSDLLRPHVATIRNNAWILGHLDFVRA
                     KYLFMSDNKATIPKISDDSTLALINVRHPLLSNPVANDLHFDHDLTAIVITGPNTGGK
                     TIMLKTLGLAQLMGQSGLPVLADKGSKIAVFNNIFADIGDEQSIEQSLSTFSSHMTHI
                     VSILNEADHNSLVLFDELGAGTDPQEGASLAMAILEHLRLSHIKTMATTHYPELKAYG
                     IETNFVENASMEFDAETLSPTYRFMQGVPGRSNAFEIASRLGLAPFIVKQAKQMTDSD
                     SDVNRIIEQLEAQTLETRRRLDHIKEVEQENLKFNRAVKKLYNEFSHERDKELEKIYQ
                     EAQEIVDMALNESDTILKKLNDKSQLKPHEIIDAKAQIKKLAPQVDLSKNKVLNKAKK
                     IKAARAPRIGDDIIVTSYGQRGTLTSQLKDGRWEAQVGIIKMTLTQDEFTLVRVQEEQ
                     KVKSKQINVVKKADSSGPRARLDLRGKRYEEAMQELDHFIDQALLNNMGQVDIIHGIG
                     TGVIREGVTKYLRRHKHVKHFAYAPQNAGGSGATIVTLG"
     gene            complement(25404..25949)
                     /locus_tag="VGT87_000396"
     CDS             complement(25404..25949)
                     /locus_tag="VGT87_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEQ3813284.1"
                     /translation="MLSLLIVLVLTWNFYIGYSRGIILQSFYVLGALLSLLVANRFYI
                     GLAHKLTLWIPYSNPVEGTSVFFFKSVDIFVLDKVYYAGLAFFIIFLLGYALSRFLGI
                     FVHFLLLDYFDNQWTKCLSGGLALLVSLLFLNMLLSIFATVPMPFLQHYLHSSFLARL
                     VIEHLPPLTIIIQKLWIQAII"
     gene            complement(25952..26260)
                     /locus_tag="VGT87_000397"
     CDS             complement(25952..26260)
                     /locus_tag="VGT87_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813285.1"
                     /translation="MKSINRYKFTFGEKTLTLTTDKDNLFMEEVERVAKEKYQALKNH
                     LPEADDETIAILMAINTLSTQLSREIAIEKMEAEILDLRQKTLVGLQEKANQIDVNEV
                     "
     gene            26417..27319
                     /locus_tag="VGT87_000398"
     CDS             26417..27319
                     /locus_tag="VGT87_000398"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEQ3813286.1"
                     /translation="MNTLVLKIDAILSKHLKKQLAPYTISSQNTYVAFAAKKNGVTVL
                     LYKSGKLVLQGNGANALAQELNLPVAKTVFEASNNSQDIPIIGSDEVGNGSYFGGIAV
                     VASFVDPKDHPFLKKLGVDDSKKLSDKTIQQIAPLLEKQIPHQSLLLSPKKYNELVGK
                     SKPYNAISIKVALHNQAIFLLLQKGIQPKQIVIDAFTSQSNYEKHLKKEKNHFPNPLT
                     FQEKAESHYLAVAVSSIIARNLFLDNLDQLGQDLGYQLPSGAGSASDKVASQLLAAYG
                     MSSLEYSAKLHFANTHKAQALLTK"
     gene            27330..27923
                     /gene="lepB"
                     /locus_tag="VGT87_000399"
     CDS             27330..27923
                     /gene="lepB"
                     /locus_tag="VGT87_000399"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEQ3813287.1"
                     /translation="MKQFIKEWGPFTLFLILFGLSRLFLWQAVKVDGHSMDPTLAHGE
                     RLIVFNQARIDRFDIVVAQEEENGQKKEIVKRVIGLPGDTISYNDDTLYINGKKTVEP
                     YLAEYLKQFKNDKLQKTYAYNTLFQQLAETSDAFTTNSEGQTRFEMSVPKGEYLLLGD
                     DRIVSRDSREVGSFKKENLIGEVKARFWPLNKMTVFN"
     gene            27981..30434
                     /locus_tag="VGT87_000400"
     CDS             27981..30434
                     /locus_tag="VGT87_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEQ3813288.1"
                     /translation="MEYVFTGTVDRIIFENQANFFKILLLAIEDTDSDIDDFEIIITG
                     TMADIIEGDDYTFWGELTQHPKYGQQLKLSRYQKIKPSSSGLVNYFSSDHFKGIGKKT
                     AEKIIALYGHNTIDHILEDPSKLETISGLSKANRQAFVAKLKLNYGTEQLIAGLVELG
                     LSNRFAFQAFEKYKEEALDLVKENPYQLVEDLQGFGFKMTDALAENLGIESDSPKRFR
                     AALLHCLLEESINRGDTYVQARQLLDFAITLLEDARQVECDPAAVAEQLSELIIEGKI
                     KNSDTKLFDASLYFAEEGIANNISRLLDTPLSQSFSHDTIQTTIQAVQKDFAITYDQV
                     QQEAITKALTSKVFLLTGGPGTGKTTVIRGILQAYANLHQIDLDKKDLPILLAAPTGR
                     AARRMNELTGLPSATIHRHLGLNGDNDYQAMEDYLDCDLLIVDEFSMVDTWLANQLLG
                     AINSTTQVIIVGDSDQLPSVGPGQVLSDLLKVNSLPQIALQKIFRQSQESTIVNLADQ
                     MRRGILAADFRDKKADRSYFEAQAAFIPDMIQKIVLSAIKSGIPAEEIQILAPMYKGQ
                     AGINHLNQLMQELLNPLQGQTEFLFNDTHFRKGDKVLHLVNDAQLNVFNGDIGYITDL
                     IPAKYTESKQDELILDFDGSEVTYPRNEWLKLTLAYAMSIHKSQGSEFQVVILPITRQ
                     SGRLLQRNVIYTAITRSKSKLILLGEYTAFEYAIKHEGDKRQTYLIERFQEQSDLASS
                     QPNQELKSKEQTSLFSNTATLEDDSQKSSSQSTNANPTENSQSDNDDFRLTPENYSTI
                     DSMIGLTESDIALFFQKKS"
     gene            30525..31007
                     /locus_tag="VGT87_000401"
     CDS             30525..31007
                     /locus_tag="VGT87_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813289.1"
                     /translation="MISYEKVRQALKTSTIAIIILNGLGVVLSLMGFAGIFYLQSQLK
                     NEAFRAQLTTEQLAQLQSSMTPFMIFLSVLNVLAIIAIIVFCAQNLSKLKQGLTVSYI
                     PYILGLILSVIGLVNQFTTTLSMVGTILILIQAALYGFAFYKAKTLNEKGDDTDQAML
                     "
     gene            complement(31100..32194)
                     /gene="dinB"
                     /locus_tag="VGT87_000402"
     CDS             complement(31100..32194)
                     /gene="dinB"
                     /locus_tag="VGT87_000402"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEQ3813290.1"
                     /translation="MLIFPLINDTSRKIIHIDMDAFFAAVEERDNPALKGKPVVVGKD
                     PRETGGRGVVSTCNYEARKYGIHSAMSSKEAYERCPKAIFISGNYEKYRTVGDQIRRI
                     FKRYTDLVEPMSIDEAYLDVTDNKLGIKSAVKIAKLIQHDIWKEVGLTCSAGVSYNKF
                     LAKLASDFEKPHGLTLVLKEDALCFLAKLPIEKFHGVGKKSVEKLHDMGIYTGQDLLA
                     VPEMTLIDHFGRFGFDLYRKARGISNSPVKSDRIRKSIGSERTYAKLLYQETDIKAEI
                     SKNAKRVAALLQDHKKLGKTIVLKVRYADFTTLTKRVTLPELTRNAAQIEQVAGDIFD
                     SLSENPAGIRLLGVTMTNLEDKVADISLDL"
     gene            32404..34731
                     /gene="pflB"
                     /locus_tag="VGT87_000403"
     CDS             32404..34731
                     /gene="pflB"
                     /locus_tag="VGT87_000403"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEQ3813291.1"
                     /translation="MATVKTNTDVFEKAWESFKGTDWKEKASVSRFVQANYTPYDGDE
                     SFLAGATERSLHIKKVIEETKAHYEATRFPYDTRPTSIADIPAGFIDKENELIYGIQN
                     DELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIRRA
                     RHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAITEIDEESIRLREEV
                     NLQYQALGEVVKLGDLYGVDVRRPAQNVKEAIQWVNIAFMAVCRVINGAATSLGRVPI
                     VLDIFAERDLARGTFTESEIQEFVDDFVLKLRTVKFGRTKAYDALYSGDPTFITTSMA
                     GMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDQLPETFRRYCMKMSHKHSSI
                     QYEGVTTMAKEGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNGGY
                     DDVHRDYKVFNVVEPITSEVLEYDEVMANFEKSLDWLTDTYVDALNIIHYMTDKYNYE
                     AVQMAFLPTHQRANMGFGICGFANTVDTLSAIKYATVKTIRDENGYIYDYEVTGDFPR
                     YGEDDDRVDDIAKWLMEAYHTRLASHKLYKNAEASVSLLTITSNVAYSKQTGNSPVHR
                     GVFLNEDGTVNTSQVEFFSPGANPSNKAKGGWLQNLNSLAKLEFSHANDGISLTTQVS
                     PRALGKTFDEQVDNLVTVLDGYFENGGQHVNLNVMDLNDVYDKIMNGEDVIVRISGYC
                     VNTKYLTPEQKTELTQRVFHEVLSMDDAAEAISSK"
BASE COUNT        10585 a         7445 c         6209 g        10507 t
ORIGIN      
        1 taaagttatc agagaaacag agagtcataa ttttatcctt ttttctcata ttgataccat
       61 ggtattaaac gagttgattg gggtgactca caatcatttc taattgacca tcaaatgacc
      121 acgatttaac atcgttaggc aagataaagt gcatgccttt ttctagctca taagccttct
      181 ggtccacata gagttttcct tgacctttta aaacactcac taaaagataa ggcgcagctt
      241 gtttcatgtc aaccatttga ctagttaccc atttgtaaac agtgaaaaat ggcgttgaga
      301 ctaaagttgt tgctaccata ttatccaaaa ccatagtggc tggcacgcta ttttctggct
      361 taccaatcgt taagacatca atagactttt caatatgaag gtcacgaaga ttaccattaa
      421 cgtccttacg gtcaaaatca taaacccgat aagtggtatc agatgactgc tgggtttcta
      481 aaataagaat tcccttgccg atcgcatgca tggtgccgct tggaacatag aaaaaatcgc
      541 ctgctttgac tgggacacga gtcagcaaat catcccaagc gcctgcttca atcatggcac
      601 gaaggtcttc tttggacttg gcctgatggc cgtatacaat ttcagaaccc tcttcggcag
      661 aaataatata ccagcattct gtcttgccta attctccttc atgttcatgt ccataagcat
      721 catcaggatg aacttggaca cttagccagt cattagcatc taaaatctta gttaacagtg
      781 gaaaaacttc ttcctttgga ttcccaaaca aggccggctc ttgcgcatat aatgtgttta
      841 aaggttgtcc ttgataacgt ccattagtaa ctgtcgaaac accattagga tgagcagaaa
      901 tagcccaata ctcacctgtg gtgtcacttg ggatgttgta agcaaagaca tctctcaact
      961 tggtgccacc ccaaatcctg tcgtgcatgg ttgattttag aaataatggt tctgacataa
     1021 tattcctcgc ttctgatgtt gatctctgtt tattatagca tgttctagct gactgcgctt
     1081 tcttttacta gacaagcagg caaaaataga ccctattttc cttgtgcagc caacttggcc
     1141 aaagcaaaat ttcccagagt agcggagcca ttatcaacaa cagctggagt aacaatgtag
     1201 tctgtcaaat cagggactgg caaataaccg cttaaaaggg tagtgaattt atcatgcacg
     1261 cgcaacacca tatgttcttg cgccatgaca cctccgccaa aaacaatcgc ttgaggacgg
     1321 taaagcatgg ttgcttgcag cgctgcttgg gcaatgtaga aggcttgaat gtcccaaaca
     1381 tcagcttcct gatcaagccg ttcacctctc actcccgttc tggcctcaat acttggacct
     1441 gctgccatcc cttccagaca ccctttatga aaagggcaaa cacctaaaaa tcccttggcc
     1501 atatcatcag gatggagcat gacataggta tgacccgctt ctgtatgacc taatccccca
     1561 ataaagtggc catgttgaat agctcccgcc ccaataccag tacctatggt gtaatagacc
     1621 aaactttcca caccaggacg agcaagcacc tcaccataag cagagctatt aacatctgtt
     1681 gtcacatcaa aaggaatctt aaaagcagcc gaaagttgtc ctaacaaatc cacattagcc
     1741 cagcctgact taggagtggt tgtgatataa ccgtaagtct ctgacgacgg atcaatatca
     1801 attggtccaa aagaaccaat cgccattcca gccaaatcgg cctcaaaggt tttaaaatat
     1861 gcgatggttt gcgcgatggt ttcttctggt gtggtggtcg gaaattgtgt tttatccacc
     1921 actgtaaact cctcatcacc cacggcacag acaaactttg tcccaccagc ttcaatactt
     1981 ccatataatt ttcccatgtt ttctcctttt tcttgttctt ttttatcttt atgatgacac
     2041 aggctcttat caagacagcc atgtctcacg gacaagcgcc actttctggt agtctaacga
     2101 ttatttagct ttatgtcatc aaaacctata aaagactaaa aatctgggac tattcatctc
     2161 agatttttag tcttttggtt aatcaattcc agcaaacctt tatcatagtc acattttgtt
     2221 aatttaatct gaatgttgct atctaagaga gttgctttgt ttattttttt agcagctgcc
     2281 ttttctcaat acaatctttc aaaactccag cattagcatt aatagcagtt acatcaaaaa
     2341 tcttactgtt caaagaagtt tctaaagcca tctcttttat ttttaactca tcaagcatct
     2401 tttgagaaaa cttatctttt tgttgagaca actctttagc agcagttact gttttcatga
     2461 tagtgtcggc gttaggtaac ggataaccta aaatttggag ttcagggagt gaaacacatc
     2521 catattgatt tttcttattg tcaactgtta ttctccagta cttagcacta atattatcca
     2581 gtgcatggct aaatacctgt tcttgtcctg aataggttga aacagtttgc caatcatctg
     2641 atttttccaa gctcgttttc acatctgctt cagcattcag ctggcctgta aagacttcaa
     2701 gttttgcttc tttgatatag tcgtctttgc ctttactgct atcattgaaa aggcgccaat
     2761 attttgctaa actagggtct ttcatttcaa agataatcga agatttattg ttaaacgata
     2821 aaggattata ttccatgatg ccattaaaaa cttttttggc attttcatca ttttcactct
     2881 taataactgt tgcgcctgct gccaaattaa ccatcatggt tttttcgtca ccaacaatca
     2941 ctttagcagt atgaacagct tttgtcttat ctgctgggct aaagaagtca accttatagg
     3001 tctcttcttt atcagttgct agcgttttgt cagtagtggt tcccaatgtg gaatcagtta
     3061 ctttaacggt atagttctca taagaaactt taaagttatt gtaatgatag tttgaatcaa
     3121 caaagctacg tccagcaatt ttatgatttt ggtaagcctt atagtctgct tcgctattga
     3181 ttagggttcc ttgattcgtc acagtcccaa acaaaagctg gctttgcaaa tcaatgtttc
     3241 cctctgccac tggtaagcgc agactagttt tcccataggt atctgggtaa tgcccagttg
     3301 gtttttgctt gtcaaatttc acggtttgtt cattgcttcc agcatgattg ctgacagttg
     3361 atagcatagt atcaaaaatt tgtcgatttt ctgttcctgg agccaaatca agtttgttgc
     3421 cagaaatatc aactttttct aaagacgtta gagtagcggc atcaagacca gccaaggttt
     3481 cacggtcaaa acctgacaaa tctaattctt tcagaccagt taaaccagaa accttcaaag
     3541 atactggtgg gatagtagca ggttcttctt tgttatcttt tttataggtt tcaagaactg
     3601 tttccaaggt atctttgcct ggcttcatat tagcgggtaa aacagaacgg tcgagttttg
     3661 taatgcgaga taagccaatc aagtctaatt gagctaattt tttaaattta tttagacctt
     3721 ctaaactttg aatcgctgga ttatccaatc gtaatgtgcc attgaaacgt tccaaatcac
     3781 cttttctggt tccaacctgc gccatcacag cttctcgcaa agccttatct gggaaatctt
     3841 tctcatcaat cagatcatac gacttatctt ttagcataac tgtctttaat gccttggaga
     3901 cactataatc tgatgttact atattatcca actttggatt tttataacca ttctttttag
     3961 gatgagctac accatcacga tccacagcat aggagaagat tcctccctta accccacctg
     4021 ttttaggttg ccatttggca taccgttcgg cacgcgttcc tttaatttct ttaccaatat
     4081 ttggattaga ctcatcttct acattaatat cataccaaag attgccgctg cctgctcttt
     4141 cctcatagaa agaaaaacca atcatgtatt gttcaggacg aatatacttg ctataacctt
     4201 gccatcgttc ttccattgtt ttttcaggtc gattagaaac aggctcccaa ccacctttct
     4261 ctccttgtga accatagacc tgtaccagta ataaattaat ataaggagct cctcgctcaa
     4321 tcaatgggtt tttatcagcc atgtaggtgc tatccataat aaacagacgc gatttatcag
     4381 caccttttgg tccaattaat ttcccaattt cttcaaacac ttgaatagag cgttctacgc
     4441 ctgctgtatc ttcttttttg tcaacttttg gaatactatc atgttcaaca tccacatcta
     4501 agccatcaag gttgtattta taaacatatt catcaacaat agctttggct aaagctttat
     4561 ttccctctgg tgtatttggg tatttactgg tatcttctgc aataccactg ttatcacccc
     4621 cagttaggaa acgccatgga atggtacgaa tgacacgtgt cccttgcttg tttaactttg
     4681 gcacatgttt ggtggccaat tctttccaaa aaaggctata atcttttgtc caatcgtgga
     4741 aaataaaggc tagatctact tctttaggaa gctctcccat cgagttaact ttgtcttttt
     4801 ctgttggatc tgatgttttg tcatgccaag ttctaaagta accaccgtag agaggaccat
     4861 gtaacggttt catcggtatt ttctcaggaa ttttcatttt ggtaagttct tgagcttgtt
     4921 tatctgcctg ctgagctttt gctaatatct ctttgacctt ttctggtact ttttcttttg
     4981 aaagctcctc tttaaattct tttttgctat tctctgacag ataatgcaag ctatctatag
     5041 aagataattc tttctgaacc tgaacagtct tctcctccgc ttttacagta ttgagtgaat
     5101 catggtgggt cgctaaggca gctcccatca acgttgcagc acaaacacac cctagtgttc
     5161 tttttaccaa caaatgttta tccatttgga cactccttat ttttggtact aagtcatggc
     5221 ttcccagttg atataccatc tatgtaagcg cattcctata gttcgcctta agtataacaa
     5281 cactttatta aaaaaaacaa ggtacaaagt ataggtaaaa taacacaaat ttaaggtttt
     5341 taggcttttc tcattttttt gccaacctgc cattcatggc aggacaaaag agctgcttag
     5401 ttagctcttt tgtccttatt tgataagtgc tacttgatca ccaatcaaaa catgtccttg
     5461 agcaagaatg tccacgcttt gatagtcagc gctattggtc acaatcatca tggtggtgtc
     5521 atctagtcca gcttctgcaa tcttgctagg atcaaagtgt ccaagaagat cacctttttt
     5581 aaccgcctga cctactgcta caagagattc aaagccatct cctgccatcg acacggtatc
     5641 aataccaata tgaagtaaca cttctgctcc ttgacttgac gttatagcat aggcatgacc
     5701 tgtttcaaag acaatttcaa ctttaccatc aactggtgag tatagggtat tatcttctgg
     5761 cttaattgct aagccttgac ccatagcacc tgatgaaaaa acagggtctg aaacagctgc
     5821 taaatctaca actgttccat tcaaaggact atataaagtt tcctctgcta aggctggctg
     5881 atcagccgtt tgatcaactt cgacagctgg taacggaagg gtttctctat cttgataacc
     5941 ccaagtataa gcaattgcaa aggctaaccc aagaccgaca agcatagtca caaggtattg
     6001 caataattgg ccattcaagt aaaggagagt tcctggtaag accgtaatcc caaaaccagt
     6061 tcctgcaatg ccaaagagtc cagcgaccca accacctaag gcaccaccaa taagacctga
     6121 aacaaagact tttggataac ggagattgac cccaaaaata gctggttcag taatccctaa
     6181 aagagctgat aaggtcgatg gaaaagccaa accttttaat tttggtgatt tggttttaac
     6241 agcaacagct aaggtagctc cagcttgagc agctgttgct gctgttaggt aagcattgaa
     6301 aggatctttt ccggtattgg caattagctg cgcttctagg aagttaaaga tatggtgaat
     6361 accagtcact acgattaatt gttggattcc cccaacaata agaccagcaa tgccaaacgg
     6421 caaatgcaag acggcctgtg tcccagccag aacaaggttt tcaagagaat ggaagactgg
     6481 tccaatcaca aatagtccca agatactcat aatagcaaac gttaaaaacg gtgtgaccaa
     6541 taagtccaaa gcttctggaa cctttttgtg caaccatttc tccaacttag ctcctaccag
     6601 accaacaaag aaggcgggta aaacggtacc ttgataacca acaacaggaa caaatccaaa
     6661 gaaggttagc ggcttaacat ctccaccaga ggcaactacc caggcgtttg gcaattcatt
     6721 ggacaccaac atcaagccta aaacaatacc gataatagga ttacccccaa agaccctaaa
     6781 ggctgaccaa gccaccaaag ctggcaagta gacaaaggct gtatccgtta gaatacgagt
     6841 gtacatgaga aaattttccc cgtactcatg caccccaaat aaatccataa tagctggctg
     6901 ggtcaccaaa ccacgaaccc ccataaagag acccgttgct acaatagctg gaataatggg
     6961 aacaaagaca tctccaaacg tacgaatggc ccgttggaag atattgcctt gtttgcctgc
     7021 ttctgctttt tgctcactgg ttgatgacgt gggtaaacca agagcaacaa cttcgccata
     7081 aatgttatta acggtaccag ttccaaaaat catttgatat tgaccagaat taaagaaagc
     7141 tcctttaacc ttgtcaatag cttctgcttt ttccttgtca atctttcctt cgtcatgaac
     7201 cattactcga aggcgggttg cacagtgggc aacactcctc acattttctc ggccccctaa
     7261 agcctcaatc acttcagttg caatctgacg attatccatg cctaaaagtc tcctttttca
     7321 tttggtgtta gtccctcatc cattctgcca gctttgtggc taagaatgtg taggaccaac
     7381 gctagtgaat ccgcttcact tcttgactat cattttatca ctaatatttt ttatgtcaag
     7441 cgtttaacat atttttattt tatatgtttt aaattcttga tttttaaaca gaaaacgttt
     7501 agtatattct tgaagacgac tataaaacaa ggagattttg atggatctac cacaagctat
     7561 tcgctaccgc ccttacaaag agtggagctc aaaagactac caagctatta ctgaaaaaat
     7621 ggcccaatcc ccatggcaca gccaatttca tgttgaacct aagacaggat tactcaatga
     7681 ccccaatggg ttttcttatt tcaacggtcg ctaccatctt ttttatcaaa actggcctta
     7741 tggcgctgcg catggtctga aacaatgggt acacatgaca tccacagacc tcgtccattt
     7801 tacggaaaca aggagccgcc ttctgccaga ccacgctcac gatagtcatg gggcttactc
     7861 aggttctgcc tatgctattg atgacaagct cttcctcttt tacactggta atgttcgcaa
     7921 tgccaactgg gtcagaacac cattacaggt tggcgcatgg atggataaac aaggaaatat
     7981 ttccaaaatc ccgcaggttt tgattgaaca gcccgatgat gttacagaac attttcggga
     8041 tcctcagctt ttttcttatc aaggtcaatt ctatgctatt attggcgccc aaggtttaga
     8101 tggcaaagga aaaatcaaac tttacaaagc tgtggacaat catgtggaca actggcgatt
     8161 tatagccgat ttggactttg acgattctgg tacggaatac atgattgaat gccccaacct
     8221 tgtgtttgtc gatgacaaac ctgttttgat tttcagccca caaggactgg ctaaagctga
     8281 tttagattat caaaatatct accccaatac ctataaaatt tttgaaagct tcaatccaga
     8341 aactggccaa ttgctgggtg ggggggccct tcaaaatctt gactttggtt ttgaagctta
     8401 tgccactcaa gcctttagta gtccagatgg cagggttcta gccgtgtctt ggattgggtt
     8461 accagatatt gattatccga cagatcgtta taactatcaa ggagctctta gtttagtgaa
     8521 agaattaagg attaaagacg gaattctcta tcaaacacct gtttcagctc tacaaaatct
     8581 tcgagggcca gcagaattat ttcataataa aatagacagt tctaactgtt atgaattgga
     8641 attgacgatt ccaggccaaa aaaagcttga cctgctccta tttgctgacc aaaaagggaa
     8701 tggcctaaga ttgaaagtgg atacaacaaa aggacagtta agcattgacc gaagtcgggc
     8761 aggggttcaa tatgcccaag actatggtac ggtgcgctct tgccaaatcc cacaaggcca
     8821 tgttacactt aatgtctatg tggacaattc cattcttgaa atctttatta accaagggca
     8881 aaaagtccta acgagtcgtg ttttcccaac ccaaggacaa acaggtatcc aagtagtaga
     8941 aggacaagct tttggacatt attatgaaat gaggtattag gcaagtggtc gcaaaattaa
     9001 cagacgtggc agccttggcg ggtgtcagtc caacaactgt ttcgcgagta atcaacaaaa
     9061 aaggctacct ttcccaaaaa acagtgaata aggtgaataa ggccatgcgt gaattgggct
     9121 acaagcctaa caatctcgct agaagtttgc aaggtaaatc aacccaactc attgggctta
     9181 tcttccccaa cattagcaat atcttttacg ctgaattaat tgaacatttg gaaattgaat
     9241 tgtttaaaca gggctacaaa actattattt gcaatagtga acataatccc gtcaaagagc
     9301 gcgaatactt agaaatgctt gcggccaatc aagtggacgg cattatttct tccagtcata
     9361 atttgggaat tgaggattat gagcgtgtcg aagctcctat tgtagctttt gaccgtaatc
     9421 tggcgccaaa tatcccggtt atttcttcag ataattttga aggtggtaaa ttagccgcac
     9481 aaactttgca aaaacacggt tgtcaaaaca ttgtcatgat tacaggtaat gacaattctg
     9541 attcgcccac agggttgcgc cagctagggt ttaactacca gctcaaacga agtgcagaaa
     9601 tcctcaaatt accaaataac ctatctcctg ttagacgcga aatggaaatt aaatccattt
     9661 tggcaacccg aaaacctgat ggccttttcg tttctgatga tttaactgct atcctcatta
     9721 tgaaagtagc caaacaactt cacattacta ttccagagga tatgaaagtc ataggttatg
     9781 atggaacgac atttatccag caatatgttc ctcaattagc gaccattcgt caacctattg
     9841 atgagattgc taaattgagt gtcgaaatac tcatcaaaaa aattaaaaaa gaaaaaacca
     9901 gtaaagacta tattttaccg attacgctac ttccaggagc aagtatttaa acaaaacata
     9961 accaatcact aattactcta aaagacaaac aagcatactc tttctagatg cgagagtatg
    10021 cttgtttgtc ttttaatatt cgtacataaa ttgaaaaaat cggatacaat cctaagattg
    10081 ctccgactta ttatgattta tttgctgaag gagcttccga gacatattgg ctaagtaagc
    10141 cattaataaa tttagcagag gtttcatcag aatatttttt agctacttca atgatttcgt
    10201 ttagtgcaac acggtctggt gtttcgtcaa aatacttgat ttcaaaaaga cctagtcgta
    10261 ataacgtttt gtctgttagt gtcaagcgtt ctaacgacca accttttttc aaatgagttg
    10321 atatcaagtt atccaactct tctttatgat tattgacacc cgttacaaga cttaataaaa
    10381 aaatcggcag ctccaatact tgagcgtcct cccctgttac tttatcataa ccataggcaa
    10441 actgagaagc tgccaataac tcagctccca tctctatgtt aaataaagcc tgaaaggcac
    10501 gttcacgcaa atctcttctt gaattttgaa agctgttagt catccaagaa atcctcatca
    10561 aaaagtgact tcaaatctgg ttttggtgtc ttttccgcaa caataccttc aacatggata
    10621 ttaactgcag aaataggcac ttctgccata tcgtacacag cagatttgac tgttttttga
    10681 atattcatag atacagtcgg gaccttgact ccatattgca agtaaacata aatatctgct
    10741 gtaacacttc catcttcttc tgtctggaga taaacccctt taccaagact tgctttgtta
    10801 aagctatctg ccattttctt gttatggagt gagtggacgc cctcaacttg tgtcgtagca
    10861 atccccgtaa taacctcaag cactcgtggt gaaatgacga tttcaccgat atattcagtt
    10921 gtcatagcat ttcctttctc ttttttagac taatctgttt tgacaaagga ttaagcacga
    10981 gaaacgtaag taccttctgc agtgttaatg attagttttt ggccagcttc gataaagtct
    11041 ggaacgttaa caacaagtcc tgtctcaaga gttgcaggtt tccctgaacc cgtcactgtc
    11101 gctcctttaa tagatggttg tgtttccgca acggtcaatt caacagttgt tggaaccgtt
    11161 accccaatca cttcacttcc ataaaattgg attttcacgt ctgagttttc aagaatgtaa
    11221 agcaattctt gctcaacgtt agcaactgga atttcgtact gatcataagt atcagtgttc
    11281 atgaagtaag cagtgtcatc cattttgtat aggtattgtg ctgggacagt ttcaatgatg
    11341 gcttgctcaa atttttcatc tgggcggtaa gttgtgtcaa aagtagaacc tgtacgcaca
    11401 tcacgtagtt tcatacgcat gatagtgttt cctttacctg gtttgtggtg gctagcttca
    11461 aggacacgaa ttaattttcc ttctgcttca aatgtcatac ctgctttaag cttacttgct
    11521 tcaatcatta ttattacctc ttactaaaat atttatcact tcattttacc acaaaagtgg
    11581 ccttggctca aatcttttat gaccgtcctt ttaggtacac tttctattgt tccttatcgc
    11641 ctcctaaaac aatttgaggc acatcatgtt gcacataggc aacccctttt ttctccatca
    11701 gttcaatggc aaaggggtgc ggacggtaat gtgctttata ggtaatcttt gtaatccccg
    11761 cctgcaaaag cgccttagtg cagttaatac aaggaaaatg agtcacgtaa atttcagttc
    11821 cgtccgttga aattccttct ttcgcacatt gaatcaaagc attcatttca gcatgaacag
    11881 tgcgaataca atgaccgtct tccatataat gacccgcttc gttgcagtta tccgtagcag
    11941 aaactccgcc attgtaacct gtggcaataa tgcggttatc ctttaccaaa acagctccca
    12001 caaaggcgcg atcacaggta gagcgcttgg aaatcagctc tgcattggcc ataaaatagt
    12061 cttgccatga taaacgattt gtcattgctt ctcccctaat cctttcctga accattcttt
    12121 tacaatacaa ttaattcttt gggtgccaag gtcaagactt gacaaccagt ttttgtgata
    12181 accaagtcat cttcaatacg gacaccatat ttgttatcca aatagatacc tggctcgtct
    12241 gtgaccacca ttccagcttg gagaagttgc tcagatttcc ccaaaaatgg attctcatgg
    12301 atgtcaagcc cgataccatg accaatgcca tgtgtgaagt gactgccata acccgcctca
    12361 gtgataagtt ggcgcggaat accgtcaaag tcactataag tcatgccagc gctagcttta
    12421 gcaattagag ccttattagc agcaagaaca agagcataaa tctcacgttc ttcatcagta
    12481 acttggccaa tgtgaatggt cctcgtcata tcactaacat agtgattgta gtaacaccca
    12541 aagtccatgg tcaagctctc tttattctgg ataaccttgt cactggcgcg tccatgaggc
    12601 atggcagagc gatagcctga agctgcaatg atatcaaatg atgtgccgct ggcaccatac
    12661 tgacgcatgc gaaaatctaa aaaattagcc aggtcacgtt cagtggttgt ccctggttta
    12721 ataaaatcaa gagcatcttc aaatgctttg tctgagatcg agcacgcttt agcaatggtc
    12781 tcaatttcag agacgtcctt aataagacgt aaatgctcca caaagcctga ctgagcaagc
    12841 aaggttattc ctgacagttc tgcttgcatg gcctgataaa aagaaaacga tacctggtcc
    12901 tcaaaaccaa ggcaatctat ttgatcagcc cctaacaatt ctgccacaac cttcaggggc
    12961 gtgcggcttt cgataatatc aaatccctca acactagctt tagcaagcaa ggtataacgt
    13021 gaatctgtga tcaaaacacg acgtttggcc gttatcaaaa cagttgctgc agttccagaa
    13081 aaacctgtca agtaataact attggttaaa tgggtgacta gaagagcctc tagccccttc
    13141 tctgccatct gccttaggca gtgacctaat cgttgctcta aaaatcctga catcgcgaac
    13201 tcctttggta acttttgaga atattgtagc atgaaaaaac tactttaagt ttaccatggt
    13261 ttgagagcat ttaaaagatt aaaaaggagc tcaaacgaac tcctaatcct attgttgtag
    13321 ttttactttc aaataatgac cagtatagct ttctttaact tgagctacct cttctggtgt
    13381 ccccgtggca acaatttgac cacctccgac accaccttct ggtcctaagt caataatatg
    13441 gtcagcagat ttgataacgt ccaaattatg ctcaatcaca agaaccgtat taccatcgtc
    13501 tacaaagcgt tccaaaactt ttaatagcct tgcaatatca tctgtgtgta aaccagtcgt
    13561 tggctcatct aggatataca agctcttacc tgtcgagcgt ttgtgcaatt cactagctaa
    13621 tttcatccgc tgcgcttctc ctccagatag agtagtagct ggttgaccca aggtcacata
    13681 acccaaacca acatccttaa tggtttgaat ttttcgggca attttaggga tagcagagaa
    13741 aaataccaag gcatcatcga cagtcatgtc aagcacttca gcaatatttt tttctttata
    13801 atgcacctca agcgtctcag aattataccg tctgccgtgg caaacctcac aaggcacata
    13861 aacatctggc aaaaagtgca tttcgatttt gataatccca tcacctgaac aagcctcaca
    13921 acgcccacct ttgacattaa aagagaaacg gccttttttg tagccacgaa tcttagcttc
    13981 gttggtttgg gcaaacaagt ctcgaatatc atcaaagaca cccgtatagg tggctggatt
    14041 tgaccgtggc gtccttccaa tcggactttg gtcaatgtca atcaagcgtt caatatgttc
    14101 aataccagaa atagagtgat atttaccagg cttatcagca ttacggttta atttttgggc
    14161 gacagccttt ttcaaaatac tattaaccaa ggttgatttc cctgagcctg atacccctgt
    14221 gaccgcaata aatttaccca gtgggaatct cacgtcgagg ctttgaagat tattctgcgc
    14281 tgctcctttt atttcaatga atcgcccatt accagaacga cgctctaatg gcactggaat
    14341 gaattttttt ccagacaaat attgacccgt gatggatttt ttattcttag ccacttgttt
    14401 cggcgttcca gaggcaataa tttctccacc gaactcacct gccccaggcc ccacatcaat
    14461 cagccagtct gcctgcatca tggtgtcttc atcgtgttct accacaatta aggtattacc
    14521 caaatcacgc atttttttca ggctttcgat caaacggtca ttgtctcttt ggtgcaatcc
    14581 aatagaaggc tcatccaaga tatagagcac ccctgataag ttagaaccaa tctgtgtcgc
    14641 taacctgatt cgttgacttt ccccacctga taaagtccca gcagcacgtg ataaggtcag
    14701 ataattcagt cccacattat ttaagaaggt caggcggtca tgaatttctt tgacaatcgg
    14761 tttagcaatg gtactttcat tttctgtgag ttccagttct tccaacaact gcaagtggtc
    14821 ggcaatcgat aattctgaaa tttgaccaat atgaggtcct tcttcgccac ccacatgcac
    14881 acacaaggct tggtcattca aacggtaacc atgacaagtc gcacaggtca attcgttcat
    14941 ataaccacgc atcacattgc gcgtataatc actattagtt tcgtgataac ggcgattaac
    15001 attagtcaca acaccttcaa aaggaatgtc aatgttacgc tcaccaccaa aatcattcac
    15061 atagtgaaaa tgaaattctc tatcccctga accataaaga actaaatccc tttcttcctc
    15121 agtcaaggcc tcaaaaggtg tatccatatc caccccaaaa cttgccatag cttgctcaag
    15181 catggttggg taataattgg aggaaatagg attccaaggc gccaaagctc cctctttcaa
    15241 agacttactt gggtcaggaa caaccaagtc caaatccact tccaacttaa tgcctagccc
    15301 atcacaggtc ggacaagagc cgaacggcgc attaaaagag aagagtcttg gttctaattc
    15361 aggaaccgta aacccacaga caggacagga ataatgctca gaaaagagca gttcattgcc
    15421 atccatagtg tcaatcataa ggtagccatc tcccagcctt aaagctgctt caaccgaatc
    15481 aaataaacgg ctacgaatac catccttatt aaccagacgg tcaataacca cctcaatgtt
    15541 gtgcatctta cttttggaca actctggtac tcctgtcacg tcaaaaatat ccccgtcaac
    15601 gcgcacacgg acatagccgt ctttttgaat tttctcaaaa actgttttgt gttgcccctt
    15661 cttccgacga acaacaggag ctaaaatctg catgcgtgtc cgttcaggga gggctaagac
    15721 ctgctctaca atttgttctg cagaagacgc cgtgatagca ccatgcccat tgatacagta
    15781 aggtgtgccc acacgcgcat atagtaaacg taaataatca ttgatttccg taaccgtacc
    15841 aaccgttgag cgagggttct tactggtggt tttttggtca atggaaatag cagggctaag
    15901 accgtcaatg gaatccacat ctggtttttc catattaccc aagaactgtc tagcataagc
    15961 tgataaactt tctacgtaac gacgttgccc ttccgcataa atagtatcaa aggctagact
    16021 agattttcct gaccctgata accctgtcac gacaactaat ttgtctcgtg gaatttccac
    16081 gtcaatattt tttaaattgt gggctcttgc cccatgaatt attattttat tttgcatctt
    16141 gaatgtgcct aaaggcaccc tcctcatcga gctttcttat attataacaa aaatttctga
    16201 taacctattg ccccaaactt tgcgaatttt ggtataatag ataagtattg agaatttttc
    16261 agaaaatcct tgagaaggct ttattatcaa ggtttgatgt caatctctac cagtcaatca
    16321 tcaaaaagga gggcttgtca tgaaacaaat gtttctttca tcggcaattg aattcaaaga
    16381 aatagaaaca ttcgagccag gagcttggat taaactggtt aacccttccc aagaagagtc
    16441 tatgaagatc gccgatcaat tcaacattga tatttctgac ttgcgcgccc ctttggacgt
    16501 ggaagaaacc tcacgtattg ccgtagaaga tgattatacc ctaattatcg tagacgtccc
    16561 gatatacgaa gaacgtaaca ataaaagtta ctatattact atgcctttag gcattattgt
    16621 aacggaaaat gctgttatca caacttgtct gcatgacatg acccttttcg atcattttca
    16681 caaccgccgg gtcaaaaatt tctatacctt catgaagaca cgttttgttt ttcagattct
    16741 atatcgcaac gccgagctct ttctgactgc cttgaggacc attgatcgcc aaagcgaacg
    16801 tttggaagct cagcttgaag cagctactcg aaatgaagaa cttatcgaca tgatggaact
    16861 tgaaaaatcc attgtctact taaaggcttc cttgaaattc aacgaacgca tcgtcaagaa
    16921 attatccagc tcaacaagct ctctcaagaa atacattgag gacgaggatt tgctagaaga
    16981 taccttgatt gaaacccaac aggccattga gatggctggc atttatgaaa atgtcttgaa
    17041 tgccatgact gaaaccactg cttccattat caacaataac cagaacacca ttatgaaaac
    17101 attggccttg atgaccatgg ctttagatat accaactgtg attttctccg cctatggtat
    17161 gaatttccaa aacaactggt taccactaaa tggcctagaa catgcttttt ggtatattac
    17221 cttgattgct atgctcctta gttcctttgt tgtgatttac tttattagaa aaaaatggtt
    17281 ctagagtgga gcaagctgca gaatgcggtt aacacctgac aaaactctgt gtcaaacgac
    17341 tggctccaaa gtctcatttg aaactagcta ataccattga cttactttaa ctaacccata
    17401 taaagaaggg attcccatgg aattacaaga acttaccaaa aaaaatcaag aatttatcca
    17461 caccgctacg aacaaattaa tccaagacgg caagtctgat gaggacatta agctgattct
    17521 cgaagaagct attccagcta ttctagaaaa ccagaaaaaa ggtgtcaccg ctcgaaatct
    17581 attaggcact ccgactgctt gggcagcttc ttttagccaa gaccctagtc aaagagcagc
    17641 agaaactgac aaaaacacca atccttggtt gatgtggcta gatacctctt tgctattcat
    17701 cggaattgtt gcccttttaa atggcatcat gaccttcttc aacacaaacg ctacagttac
    17761 aggattgatt tccttactgg cacttggttt tggtggagga gcatccatgt acgctactta
    17821 ttactttatt taccgtcatc tcggaaaaga taagagcctt cgtccaagtt ggttcaaaat
    17881 catcgcagcc ttatcattag ccatgttgat ttggattgcc ttgtactctg caactgcctt
    17941 tttaccaacc tctcttaacc cacagttgcc tcctcttgcc ttgctcatta ttggtggtgt
    18001 ttcccttgct ttacgctatt acctacaacg caaatacaac attcaaaaca ctatgtcacc
    18061 tgttaacaaa taacaatcat ttcatccttg ctgtctacgc aaggattttc gtttactatc
    18121 attctctctt ttagaaaaaa ctcacctata cgcctagact agtcctagtc aactaaaaaa
    18181 cagtccgaaa tggactgttt gtttattagt cttcgtttac gtaaggcata agagccataa
    18241 cgcgagcacg tttgattgct gttgttactt tacgttggtt tttagctgaa gttcctgtta
    18301 cacgacgagg aagaattttt ccacgttctg aaacgaaacg gctaagaagc tcagtatctt
    18361 tgtaatcaac atattcaatt ttgttagctg cgatgaagtc aactttttta cggcgtttga
    18421 atccgccacg acgctgttga gccatgtatt ttctccttta taaattatga tgttaagggc
    18481 tcttaaaaaa cgactgaatt ttagaaaact ggcaagaaat cttgatttcc tagggagttg
    18541 atctaaattc taagttctaa atttttagcc cgaactcaaa taatttggta ttcgttcaat
    18601 ccattctgac tagaatggaa gatcgtcatc tgagatatcc atcgggtttg agttcccaaa
    18661 tgggctatca tctcttccaa agttaggcgt ttgttgtgca ggcgctgagt aactgtttga
    18721 tgatgaagtg ttattgttaa aaccaccatt aaatgagcca gttgagccac cttcacgtgt
    18781 agcacgactt tccaacattt ggaaattatc tgcaacaact tctgttacat agacacgttg
    18841 tccttgttgg ttttcgtagt tacgtgtctg aatacgaccc gtaactccga tcaaggcacc
    18901 ttttttagcc cagttcgcta aattttcagc cggttgacgc cagatcacac agttaatgaa
    18961 atctgcctcg cgttcaccat tttggctttt aaaggtacgg ttaacagcaa gtgtgaaggt
    19021 agccacagct acttgacttg gtgtgtaacg aagttctgca tccttggtca tacgaccaac
    19081 tagtactaca ttattaatca taaactaccc tcttagttgg aattaagcgt caagtttaac
    19141 gatcatgtga cgaagaatgt caccattgat ttttgaaaga cggtcaaact cgttaagagc
    19201 tgctgcgtca gtcgcttcaa ggttaacgat gtggtaaagt ccttcacgga aatcgttgat
    19261 ttcgtatgca agacgacgtt tttcccaatc ttttgattca acaacagttg caccgttgtc
    19321 agtcaagata gagtcaaagc gtgctaccaa agcgttttta gcttcttctt caatgtttgg
    19381 acgaatgata taaagaattt cgtatttagc cattgatatt ttcctccttt tggtctaatg
    19441 actcactact tttggagtga gtaagtgagg tatactcaca gaatcttatt gtacactaat
    19501 agtcaattaa aagcaaggaa ctttttctac ttgactaagt tttgtcaaac attttctttc
    19561 acgagtttta aaaattggca cttagtcaga ctcctaaaaa ataggtgaaa aaagaaaaag
    19621 caaggaaaag ttttaaccaa agtgcaaaat ataaaattag ggccaaaaca aaaaggttaa
    19681 actttttaaa aataatagca accatggcac atacgatacc taatccagga aaaacttgcg
    19741 tcatgatcag ccattccaac tgtagtccgc tcaaagaagg atcaaatgga taaatatagt
    19801 taagaaataa cacaagaatc atcattataa aaacgaacca cgaataccga tcaataagat
    19861 tctttaattt ctggaacatg tcccctccaa attaaaacct gtctgcttct atcgttaatt
    19921 tcagttattc taaatcaaca tttagatgag gtaaacctaa tattattata atatttcctt
    19981 ttctttttcg caaataccta aaatcttttt ttactctttt taaggctcct gtgttacaat
    20041 aacaacatga ttgaattaaa agattatggt attaatatgt gggataatga gacaatcgct
    20101 tcttttcgtc gcacactttt agggtggtat gaccaagaaa aacgtgatct cccttggcga
    20161 agaactacta atccatatta tatttgggtt tctgaaatca tgttgcaaca aacacaagtt
    20221 aatacggtaa tcccttatta taaacgtttt ctagagtggt ttcctcaaat aaaagatttg
    20281 gcagacgctc ctgaagaaca attactaaag gcatgggagg gactaggcta ttattctcgg
    20341 gtgcgtaata tgcaaaaggc tgcacagcaa gtgatggttg attttggtgg catatttcct
    20401 cacacttatg atgatattgc ttcattaaaa gggattggtc cttatactgc aggtgctatc
    20461 gccagcatct cattcaactt accagagcct gctgttgatg gcaatgtgat gcgtgtcatg
    20521 gctcgtttgt ttgaagttaa ttatgatatc ggtgacccta aaaatcgtaa aatttttcaa
    20581 gctattatgg aaatactaat tgaccctgac agacccggtg attttaacca ggcacttatg
    20641 gatctaggta cagacatcga gtccgctaag acacctaggc ctgatgaaag ccctattcgt
    20701 ttctttaacg ctgcttattt gaatggtact tatggtaaat atcccataaa aaatccaaag
    20761 aaaaagccta aaccaatgcg aattcaggct tttgtcattc gtaaccaaaa tggccaatat
    20821 ctactggaga aaaatactaa agggagactt ctaggaggtt tttggtcatt tcctattatc
    20881 gaaacgtcac ctctttctca acaattagat ttatttgatg acaatcaatc caaccccata
    20941 atctggcaaa cccaaaatga aactttcgaa agagaatatc aattaaagcc ccaatggact
    21001 gataatcact ttccaaatat taagcacact tttagtcacc agaagtggac aatagaactc
    21061 atagaggggg tggtcaaagc aacagattta cctaatgctc ctcacctaaa gtgggtagca
    21121 attgaggact tttctcttta tccctttgca acgccccaaa agaaaatgct agagacctac
    21181 ttaaaacaga aaaatactta atcacaagtg aactatgacg gttcactttt tgtttacaat
    21241 tcctttacgt cgctgtaaag ttttcctcag attatctttt aataccctat aaatagtggt
    21301 ctatccctaa tgtcaaatac atttgataga cccgttagct tatcatcctt atactaatat
    21361 tatgaaagga gatatttatg gaaattggtc agcaaattat tcgctaccgt aaacaacaag
    21421 cgctctcgca agaagaatta gccgagaaag tctatgtaag ccgtcagagc atatctaact
    21481 gggaaaatga caaaacttac ccagacatcc atagcctctt actcctaagt caaatttttc
    21541 aagtgagctt agaccaactc attaaaagag acattgagaa aatgaaatat accatcactc
    21601 aggttgataa aaagaatttt aaacgagata ccaaagtaat ggtaacattg atgatactat
    21661 taatgatttc aagttatcca ttagtctatt tcttagagtg gctaggactt ggcatttttg
    21721 ttttattatc gataattact atgacttatg ctaatcgtgt ggaacgtttt aagaaaaaat
    21781 atgatgtgca accttataag gaaatcctag ctgtttcaag tggaaaatta ttagacgaaa
    21841 tcgaaaaacg agaagaaaga gcaaaacttc cttatcagaa cccccttatt gtcactgtat
    21901 ttttcctcat tactgttgct attgcttttg caagtcgttt tatgttcaca tggttattcc
    21961 attgaagtta gtaggtcata aaataaagct cagcgtgacg ctgggcttta ttttagttaa
    22021 ctcaattcag caataatagc tttcagttgg tctttagtat gaacacctgc aacttgttta
    22081 accacttctc cgtctttttt aaacatcaat gttggaattg acataatacc aaactgacga
    22141 gcagtttccg gattttcatc aacatccatt ttcaaaattt tgagttcatc ttcatcgatt
    22201 tcttgagata actgttctaa gattggtgtt tgcatacgac atggaccaca ccaagttgcc
    22261 caaaaatcaa tgagtactaa accttcttta gtttcttcta caaatgtagc atctgttact
    22321 tctaatgcca tcttttttct ccatttcatc ttaatgataa gcctattgta caagaattta
    22381 gttgtttttg aaactatttg ctacgaactc gctaaatcaa taaacaaagc cctaacatca
    22441 agccaataag gtaacctaca ataacatcct taggatagtg aatacctgct atcacacggc
    22501 aaattgccaa taaaaccgat aatattaaac aaactattcc aaagtaaaca taatatcgta
    22561 acaaacacat actaatcatc gtagctgaaa aaacatgtct gctaggcatt gaccttccct
    22621 ttgtatcctt atcaattaaa ggtttaatat tccatttttc atacggtcgt ggaaaatcaa
    22681 agcgtttcct aatgtaagac aaagctataa agccaacagc aggaataatt aatagcctta
    22741 accataattg aaatgtcatc ccttgccaaa gcgtaaatat cagaaaagat ggataaagta
    22801 tgtacatcac tcctgtgatt atttttagta aaacatttaa aagaattatt aactgtgggg
    22861 attttctaaa aggttgagat agtttggcat aaaagtgttc atatgattcc atcatttacc
    22921 ctaacgttac aattgtggca ccagatcccc ctgcattttg tggggcataa gcaaaatgct
    22981 taacatgctt atgacgacga agatattttg tcactccctc acgaataacg cctgtaccaa
    23041 taccatgaat gatatcaact tgtcccatat tgttaagcaa tgcttgatca ataaaatgat
    23101 ctaactcttg cattgcttct tcgtatcttt tacctctaag atcaagtcga gctcttggtc
    23161 cagaactatc agccttttta accacattaa tctgtttact tttgactttc tgttcttctt
    23221 ggactctaac aagggtaaat tcatcttgtg ttaatgtcat tttgataatt cccacttgtg
    23281 cttcccaacg tccatctttt aattgactag ttaaggtacc tcgctgtcca taactagtca
    23341 ctataatatc atcaccaatt ctaggggcac gagctgcttt gattttttta gccttattta
    23401 agactttatt ttttgataaa tcaacttgag gtgctaattt ttttatttgt gccttagcat
    23461 ctataatttc gtgaggtttt aattgactct tatcattgag tttttttaag atagtatcac
    23521 tctcattcaa agccatatct acaatttctt gagcttcttg atagattttt tctaactctt
    23581 tatcgcgttc atgtgaaaat tcattataga gtttcttaac cgcacgattg aatttgaggt
    23641 tttcttgttc aacttcttta atatgatcca gtcttctacg tgtctcaagt gtctgtgcct
    23701 ctaactgttc aataatacgg ttaacatctg agtcagaatc tgtcatctgc ttagcttgtt
    23761 taacaataaa tggagctaaa ccaaggcgag aagcaatttc aaatgcattt gagcgtccag
    23821 gaactccttg cataaagcga tatgtggggc taagcgtttc tgcatcaaat tccatgctcg
    23881 cattctctac aaaatttgtc tcaatcccat aagcttttaa ttctggatag tgcgtggtcg
    23941 ccatcgtttt gatatgactt aacctaaggt gttctaaaat agccatggcc aaactagcac
    24001 cttcttgagg atccgttcct gctcctagtt catcaaagag aactaaacta ttgtggtcag
    24061 cctcgtttaa aatactgact atgtgcgtca tatgactaga aaaagttgat agactttgtt
    24121 caatagattg ctcatcgcca atatctgcaa agatattgtt aaatactgca attttactac
    24181 ctttatccgc taatactggc aaaccagact gtcccattaa ttgtgctaaa ccgagtgttt
    24241 ttagcataat cgtcttacca ccagtattgg gaccagtaat gacaattgca gttaaatcgt
    24301 gatcaaaatg taagtcatta gccacaggat tacttaaaag aggatgacga acattgatta
    24361 atgctaacgt gctgtcatca gagatcttag gtatcgtcgc cttattatca gacataaaaa
    24421 gatatttagc ccttacaaaa tcaagatgcc caagaatcca tgcattattt ctaatagtgg
    24481 cgacatgagg tcttaacaag tctgaaaatg cgtgtaaaat acgaccttct tcatgacgtt
    24541 cgtcagctct aagctgcgtt atctcttcgt ttagtgtaac tacagcacga ggctcaatat
    24601 aaacagtact tcccgaagaa gagatgtcat gaaccacacc agaaatacga ttccgataag
    24661 tattttttac tggtaggaca cttcgtccac tacgactagc gattagattt tctgataaaa
    24721 gctctgcttt ttccttaagc atatcctgta aaatctgacg aacccgtcgt tcactatttg
    24781 ttaattgacg acggatacgc tctaattctg gactcgcaaa atgttctaaa aaaccaccat
    24841 cattgatagc ttgaaaagac ccttgcagat tagggaattg ttccaaattt tcaaacaaac
    24901 gatccaaaga ttggaaagaa acattatcca aatcagaata aaagtgaatc atatccgaag
    24961 aactctgtaa aacttttttg atagccaaaa gttcttgaat attaagatca gctgaaagtt
    25021 ctaagcgttt taaactctca gagattgaac ttaggctgac tatgccaaat gagtgatgtt
    25081 caacaaaaat ctgttccatg tcagaaattt cattaaaact tttttctatt ttaggagcct
    25141 ccgtcatcgg ctccagctct aataattctt cttgggattg ttctgtcttg agataaggta
    25201 atagcaattc cttaactttg ttaaattcta actgctctaa aatcttgtta ttcatactct
    25261 tcattatacc taaaatgatg gaaaatgtgg caagaaagtc actaagttcc catgcgttct
    25321 ctttatttct aaacagtttc tcgtaaataa agaacctaaa ggtgaaactc actccaggtt
    25381 cttatattag tcacaaaacg atatcaaata atggcttgaa tccacaattt ttgaataatt
    25441 attgtcaatg gtggaaggtg ttcgatcacc aaacgagcaa gaaaactaga gtgcaaatag
    25501 tgctgtaaaa aaggcatagg gaccgttgca aaaatagaca gtaacatgtt caaaaaaaga
    25561 agcgatacta gaagcgctag tccgccactc aaacattttg tccactggtt atcaaaataa
    25621 tctagcaaaa ggaaatgaac aaaaatccca agaaagcgac tcaatgcata ccccaataag
    25681 aaaatgataa aaaaggcaag gcctgcataa taaaccttat ctaaaacaaa gatatcaact
    25741 gatttaaaga aaaagactga tgttccttct accgggttag aataaggaat ccaaagcgtt
    25801 agcttatggg ctaatccaat ataaaatcga ttagctacca aaagagaaag tagggctcct
    25861 aagacataaa acgattgaag aataatacct ctactataac caatataaaa attccaagtt
    25921 aaaacaagca ctatcaataa agataacatg attaaacctc attcacatct atttgattag
    25981 ctttttcttg aagaccaacc aaggtttttt gacgtaaatc taaaatttca gcttccattt
    26041 tttcaatggc aatttctctg cttaattgtg ttgacaaggt attgatagcc attaaaatag
    26101 cgatagtctc gtcatcagct tctggtaaat gatttttaag agcctgatac ttttctttag
    26161 ctacccgctc aacttcttcc ataaagagat tgtctttgtc agttgtcaat gttaatgttt
    26221 tttcaccaaa ggtgaatttg tagcgattaa tacttttcat agggtcacct cattagcatt
    26281 ataccgtaaa atgaaacttt cgtaaaatag tcaacaccat tgccctaacc cgtctaaaca
    26341 ttcgagtctc tgttttcttt gttaaataaa cttatgaatt aaccatagtc aactatttta
    26401 tgttaaaata attattatga ataccttagt tttgaaaata gatgctatac tgagtaaaca
    26461 tttaaaaaaa cagctagccc cctacaccat cagtagccaa aatacctatg tagcctttgc
    26521 tgctaaaaaa aatggggtta ctgttttgct gtacaaatcc ggaaaattag ttctccaggg
    26581 aaacggtgca aatgctttag ctcaagagct taatcttcca gtagctaaga cagtttttga
    26641 agcaagcaac aatagccaag atatacctat aatcggcagt gatgaggtgg gtaatggctc
    26701 ttattttggt gggattgctg ttgtggcaag ttttgtcgat cctaaagatc atcccttcct
    26761 gaaaaagctt ggggttgatg attctaaaaa attatcggat aaaaccattc aacagattgc
    26821 gcctctgtta gagaaacaaa tacctcatca atcacttctc ctatcaccta aaaaatataa
    26881 cgaattggtt ggtaaaagta aaccatataa tgccatttct atcaaagttg ctcttcataa
    26941 ccaagctatt tttttgttgt tacaaaaagg cattcaacct aaacaaattg ttattgatgc
    27001 tttcactagc caatcaaatt atgaaaagca tctaaaaaag gaaaaaaacc atttcccaaa
    27061 tcctctaacc tttcaagaaa aggcagaaag tcactatttg gctgtggctg ttagctctat
    27121 cattgctaga aatctatttt tagacaatct cgatcaatta ggtcaagatt taggttacca
    27181 actcccaagc ggtgctggtt cagcttcaga taaagttgct agccaactct tagccgctta
    27241 tggtatgtct agcttagaat atagtgctaa acttcacttc gccaacactc acaaagcgca
    27301 agcgcttctc actaaataaa aggaatcata tgaaacagtt tattaaagaa tggggcccat
    27361 tcactctctt tttaattctc tttggtctat ctcgtctttt tttgtggcag gctgttaaag
    27421 tagacggcca ttctatggac ccaactctag ctcatggcga acgccttatc gtttttaatc
    27481 aagctaggat tgatcgcttt gatattgtag ttgctcagga agaagaaaac ggacaaaaga
    27541 aagaaatcgt aaaaagagtt attggattgc caggcgatac catttcttat aatgatgaca
    27601 cactttatat taatggtaaa aaaacagttg agccgtattt ggctgagtat ctaaaacaat
    27661 ttaaaaacga taaactccaa aaaacttacg cctataatac cctattccaa cagttagcag
    27721 aaacatctga tgcttttaca actaattctg agggacaaac acgctttgag atgagtgttc
    27781 caaaaggaga ataccttctt cttggtgatg atcgtattgt ttcccgggat agtcgcgaag
    27841 ttggtagttt caaaaaagaa aaccttatcg gtgaagtgaa agctcgtttt tggccactca
    27901 ataaaatgac cgtctttaat tagaaaaaac ctgcctttgg gcaggttttc agttatcttg
    27961 tataaataga aagagttact atggaatacg tttttacagg tactgttgat cgtatcattt
    28021 ttgaaaacca ggctaatttc tttaaaattc tcctccttgc cattgaggat acagacagtg
    28081 acattgacga ctttgaaatc attatcacag gaacgatggc tgacattatt gaaggagatg
    28141 actacacctt ttggggagaa ttgacccagc accctaaata cggtcaacaa ctcaagctaa
    28201 gccgttacca aaaaatcaaa cctagttcat ctggtttggt taattatttc tctagtgacc
    28261 attttaaggg gattggtaaa aaaacagcgg agaaaatcat tgcgctatat ggtcataata
    28321 ccattgacca tattttagaa gacccaagta aattagaaac tatctctggt ctgtccaagg
    28381 ctaatcgtca agcctttgtc gctaaactaa aattgaatta cggcacagag caactgattg
    28441 ctggtctcgt cgaacttgga cttagcaacc gttttgcctt tcaagcgttt gaaaagtata
    28501 aagaagaggc tcttgacctt gttaaagaaa atccctatca gttagtcgaa gatctacaag
    28561 gttttgggtt taaaatgaca gatgctctcg ctgaaaactt agggattgaa agcgactctc
    28621 cgaaacgttt tcgtgcagcc ctccttcatt gtctcttgga agaatccatc aatcgaggag
    28681 atacttatgt tcaagcacga caattattag actttgccat cacactcctt gaagatgcac
    28741 gtcaagtaga atgtgatcct gctgctgtgg ctgagcaact aagcgagtta atcatagagg
    28801 gcaaaatcaa aaacagtgac accaaactgt ttgatgctag cctttatttt gctgaagaag
    28861 ggattgctaa caacatctct cgtctcttag ataccccttt aagtcagtca tttagtcatg
    28921 ataccattca aacaaccatc caagccgttc aaaaagactt tgccatcacc tatgaccaag
    28981 tgcagcaaga agccattact aaggccttaa ccagcaaggt ctttctccta acaggtggtc
    29041 ccggaacagg aaaaacaact gttattcgag gcattttgca ggcttacgcg aacctgcatc
    29101 agattgattt ggataaaaaa gaccttccta tcttgttagc agctccaaca ggtcgagctg
    29161 cacgtcgcat gaatgagttg actggacttc ctagcgcaac catccacagg cacttaggcc
    29221 tcaatggcga caacgattac caagccatgg aggattatct agactgcgac ttgctgattg
    29281 tcgatgaatt ttcaatggtg gatacctggc ttgccaacca gttgttagga gctattaact
    29341 caacaactca agtgattatt gtcggagata gtgaccagct tccttcggtt ggacccggtc
    29401 aagtcctgtc agatctttta aaagtcaata gcctgcccca aatcgccttg caaaagatat
    29461 ttcgtcaatc ccaagaatct actattgtca atttggcaga tcagatgcgt cgaggaatct
    29521 tagctgctga ctttcgtgat aaaaaagctg accgttctta ttttgaagcc caagcagcct
    29581 ttatccctga catgattcaa aaaattgtcc tatctgctat taaaagtggc atccctgctg
    29641 aggaaattca aattttagcg cctatgtaca aaggacaagc tggcatcaat catcttaatc
    29701 aactcatgca agaacttctc aatcccttgc aaggacaaac agaatttctg ttcaatgata
    29761 cgcattttcg taaaggtgat aaagtcctgc acttagtcaa cgatgctcag ttgaatgtct
    29821 ttaacggaga tattggttat attacagatt tgattcctgc taaatacacc gaatctaaac
    29881 aagacgaatt aatcttagat tttgacggta gcgaagtcac gtaccctaga aatgaatggc
    29941 taaaattaac cctggcctat gccatgagca ttcataaatc gcaagggagt gagtttcaag
    30001 tggtgatttt acctatcaca cgccaaagcg gccgactctt gcaacgaaat gtgatttaca
    30061 cggccattac tcggtctaaa agtaagttaa ttctgttggg agaatatact gcctttgagt
    30121 atgctattaa acacgaaggc gataaacgcc aaacctactt gatcgaacgc ttccaagaac
    30181 aatccgactt agcctcctct caacctaacc aagagctaaa atcaaaagag caaacctccc
    30241 ttttttctaa tacggcaacc cttgaggacg actctcaaaa atcttcctct caatcaacaa
    30301 acgctaaccc cactgagaac tctcagtcag ataatgatga ttttaggtta acacctgaga
    30361 attattcgac tatcgattcg atgatagggc ttacagagtc agatattgcc ctctttttcc
    30421 agaaaaaatc ctagaaaaac ggacattttt gattaatcta tggtataata tttttgtatt
    30481 aaacacatta tacaatttcg gattaaggac tctaggagga ttatatgatt tcttatgaaa
    30541 aagtgcgtca ggcactcaaa acgtctacta ttgccattat tattctaaac gggcttggag
    30601 ttgtactgtc attaatggga tttgcaggga ttttctactt acaaagccaa ctcaagaatg
    30661 aagcatttcg ggcccaatta acaactgagc aattggcaca gctacaaagc agcatgacac
    30721 catttatgat tttcttatct gtcttaaatg tccttgctat tattgctatc attgtctttt
    30781 gtgctcaaaa cttatccaaa ctaaaacaag gattaacggt tagttatatt ccttacatct
    30841 taggacttat cctttctgtt atcggcttgg ttaaccagtt cacaacaacc ttgtcaatgg
    30901 ttggaactat ccttatcctt atacaagctg ctctttatgg ctttgctttt tacaaggcta
    30961 aaacccttaa cgaaaaaggt gatgatacag accaagctat gctgtaataa aataatggac
    31021 caaagaagct gattagggaa atccctaatc agcttctttt ttctccaaaa tgaggcggac
    31081 ggatagagcc ctctttctcc tataggtcca aggaaatatc agctacctta tcctccaaat
    31141 tggtcatggt tacccctaac aggcggatac cagcaggatt ttcgcttaag ctgtcaaaaa
    31201 tatccccagc tacttgctca atttgtgcgg catttctggt taattctggc aaggtgacac
    31261 gttttgtcaa ggtggtaaaa tcagcataac gcactttgag cacaatggtc ttgcctaact
    31321 ttttatggtc ctgtaagaga gcggccacgc gcttagcatt tttactgatc tctgccttga
    31381 tgtctgtttc ttgataaagc agtttagcgt aggttctctc actgccaatt gacttgcgta
    31441 tccgatcaga cttgacagga gaattgctga tgcctctcgc tttacggtaa aggtcaaaac
    31501 caaaccgacc aaaatgatca atcaaggtca tttcaggaac tgccaacaaa tcctgtcctg
    31561 tataaatccc catgtcatgc agtttttcaa ctgatttttt accaacacca tgaaactttt
    31621 caatggggag tttggctaaa aagcacaggg catcttcttt caagactagg gtgaggccat
    31681 ggggtttttc aaaatcacta gccaatttag ccaaaaattt gttataagac acacctgctg
    31741 aacaggtcaa tcctacttct ttccagatat catgctgaat cagcttggct attttgacgg
    31801 ctgacttaat ccccaactta ttatcagtca catcaaggta agcctcgtca atagacatag
    31861 gttcaaccaa atcagtataa cgcttaaaaa tacggcggat ctggtcccca actgttcgat
    31921 acttttcata atttcctgaa ataaaaatgg ctttgggaca acgctcataa gcttccttag
    31981 agctcatggc cgaatgaatg ccatattttc tcgcttcgta attacaagtg gaaacaactc
    32041 cgcgaccacc tgtttctctt ggatctttcc caaccacaac aggctttcct tttaaagcag
    32101 ggttatccct ttcctcaact gcagcaaaaa aggcatccat atcaatatgg atgatttttc
    32161 gtgacgtgtc attaatcagt ggaaaaataa gcataagtcc ctcctttccc tattatacct
    32221 tttaggctac ctaaagccaa aaaaaataca ttctaataca gtttgtgact gtttcaacaa
    32281 tctgtattat aaaagaatct taattttgtc aagaattttg cttgggaaaa cgtttcctat
    32341 gtgttaaact tagaattgta aatgtaacag atcgctttgt tactagaatt taaggagaaa
    32401 tttatggcaa ctgttaaaac caatacagat gttttcgaaa aagcttggga aagctttaaa
    32461 ggaactgact ggaaagaaaa agcaagtgtc tcacgttttg tacaagctaa ctacacccca
    32521 tacgatggcg atgaatcttt cttggctggt gctactgaac gttcacttca catcaaaaaa
    32581 gttatcgaag aaacaaaagc acactatgaa gctacacgtt tcccatacga tactcgtcca
    32641 acttcaattg ctgacattcc tgctggtttc atcgataaag aaaatgaatt aatttatggt
    32701 attcaaaacg atgaattgtt caaattgaac ttcatgccaa aaggtggtat ccgtatggcg
    32761 gaaacgaccc ttaaagaaaa cggctatgaa ccagacccag cagtacacga aatctttact
    32821 aagtacgtga caactgttaa cgacggtatc ttccgcgctt atacttcaaa tattcgtcgt
    32881 gctcgtcatg cccacactgt aactggtctt ccagatgctt actcacgtgg acgtatcatc
    32941 ggtgtttacg ctcgtcttgc tctttacggt gctgactact tgatgcaaga aaaagtcaat
    33001 gactggaatg ctatcactga aatcgatgaa gaatccattc gtcttcgcga agaagttaac
    33061 cttcaatacc aagctcttgg tgaagtggtt aaacttggtg acctttacgg agttgatgtt
    33121 cgtcgcccag cacaaaacgt taaagaagct atccaatggg taaacatcgc tttcatggct
    33181 gtatgtcgtg tgatcaacgg tgctgctact tctcttggac gtgtgccaat cgttcttgat
    33241 atctttgcag aacgtgacct tgctcgcggt acctttacag aatcagaaat ccaagaattt
    33301 gttgatgatt ttgtccttaa actccgtacc gtaaaatttg gtcgtactaa agcttacgat
    33361 gccctttact caggtgaccc aacattcatc acaacttcta tggctggtat gggtaacgac
    33421 ggtcgtcacc gtgtgactaa gatggactat cgtttcttaa acactcttga taatatcggt
    33481 aactctccag aaccaaactt aacagttctt tggactgacc aactgccaga aacattccgt
    33541 cgctactgta tgaaaatgag ccacaaacac tcttctatcc aatatgaagg tgtgacaaca
    33601 atggctaaag aaggttatgg tgaaatgtca tgtatttcat gttgtgtatc accacttgac
    33661 ccagaaaatg aagaacaacg tcataacatc caatacttcg gtgctcgtgt aaacgttctc
    33721 aaagcccttc ttactggtct taacggtggt tacgatgatg ttcacagaga ctacaaagtg
    33781 tttaacgttg ttgaaccaat cacatcagaa gtccttgagt acgacgaagt tatggctaac
    33841 tttgagaaat ctcttgactg gttgacagat acttatgtag atgcgcttaa catcatccac
    33901 tacatgactg ataaatacaa ctacgaagca gttcaaatgg ccttcttgcc aacacaccaa
    33961 cgtgctaaca tgggattcgg tatctgtggt ttcgctaaca cagttgatac cttgtcagct
    34021 attaaatacg caactgttaa aactatccgc gatgaaaatg gctacattta tgactacgaa
    34081 gtaacaggtg acttccctcg ttacggtgaa gatgacgacc gtgttgatga catcgcgaaa
    34141 tggttgatgg aagcttacca cacacgtctt gcaagccaca aactttacaa gaatgctgag
    34201 gcttcagtat cacttcttac catcacttca aacgttgctt actctaaaca aactggtaac
    34261 tctccagttc accgcggcgt cttcttgaac gaagatggta cagttaacac tagccaagtg
    34321 gaattcttct caccaggtgc taacccatct aacaaagcaa aaggtggctg gttacaaaac
    34381 cttaactcac ttgctaaact ggaattctca catgctaacg atggtatctc attgactact
    34441 caagtatcac ctcgtgctct tggtaaaaca tttgacgaac aagttgacaa cttggtaaca
    34501 gtgcttgacg gttactttga aaatggtggt cagcacgtta acttgaacgt tatggacctt
    34561 aacgatgttt atgacaaaat catgaacggc gaagacgtta tcgtgcgtat ctctggttac
    34621 tgtgtcaaca ccaaatacct tacaccagaa caaaaaactg aattgactca acgtgtcttc
    34681 cacgaagtgc tttcaatgga tgatgccgct gaagctattt caagtaaata gtagtatcta
    34741 aatgat
//
LOCUS       DAUCLR010000011        30495 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.011, whole genome shotgun sequence.
ACCESSION   DAUCLR010000011 DAUCLR010000000
VERSION     DAUCLR010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30495
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.011"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            18..101
                     /locus_tag="VGT87_000404"
     tRNA            18..101
                     /locus_tag="VGT87_000404"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52..54,aa:Leu,seq:caa)
     gene            1184..1492
                     /locus_tag="VGT87_000405"
     CDS             1184..1492
                     /locus_tag="VGT87_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-diphosphocytidyl-2C-methyl-D-erythritol
                     kinase"
                     /protein_id="HEQ3813292.1"
                     /translation="MKHRFQVKRGVSVYLEKRIPMCAGMGGGSSDAVTIRALNQLWLL
                     TLSRKDMMDIGIPIGSAVPYCLLSGCAQVTGKGEVVCRILGLLSSWVVLVKPDFGIST
                     "
     gene            1547..1759
                     /locus_tag="VGT87_000406"
     CDS             1547..1759
                     /locus_tag="VGT87_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-diphosphocytidyl-2C-methyl-D-erythritol
                     kinase"
                     /protein_id="HEQ3813293.1"
                     /translation="MVAAIEAGNYNDGILTEMNNLLEDIFIAKRPFIQKIKEKTLQAG
                     AATTLMTGSGPTVFALCQTEKQTIIV"
     gene            1869..2312
                     /locus_tag="VGT87_000407"
     CDS             1869..2312
                     /locus_tag="VGT87_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent MarR family transcriptional
                     regulator"
                     /protein_id="HEQ3813294.1"
                     /translation="MGTLEKKLDNLVNTILLKAENQHELLFGACQSDVKLTNTQEHIL
                     MLLSQQRLTNTDLAKVLNISQAAVTKAIKSLVKQDMLAGTKDTVDARVTYFELTELAK
                     PIASEHTHHHDETLNVYNRLLQKFSAKELEIVDKFVTVFAEELEG"
     gene            2316..3035
                     /locus_tag="VGT87_000408"
     CDS             2316..3035
                     /locus_tag="VGT87_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEQ3813295.1"
                     /translation="MRYISVKNLSFQYESEPVLEGITYHLDSGEFVTMTGENGAAKST
                     LIKATLGILQPKAGRVTIAKKNKDGKQLRIAYLPQQVASFNAGFPSTVYEFVKSGRYP
                     RSGWFRHLNKHDEEHVQASLEAVGMWENRHKRIGSLSGGQKQRVVIARMFASDPDIFV
                     LDEPTTGMDSGTTDTFYELMHHSAHQHGKSVLMITHDPEEVKAYADRNIHLVRNQKLP
                     WRCFNIHEAETDDEKGGHGHA"
     gene            3028..3843
                     /locus_tag="VGT87_000409"
     CDS             3028..3843
                     /locus_tag="VGT87_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEQ3813296.1"
                     /translation="MLDILSYDFMQRAVMAVVAISIFAPILGIFLILRRQSLMSDTLS
                     HVSLAGVALGVVIGISPTITTIIVVVLAAILLEYLRVVYKHYMEISTAILMSLGLALS
                     LIIMSKSHSSSSMSLEQYLFGSIITISMEQVVALFAIAAIILILTVLFIRPMYILTFD
                     EDTAFVDGLPVRLMSVLFNIVTGVAIALTIPAAGALLVSTIMVLPASIAMRLGKNFKT
                     VILLGIVIGFSGMLSGIFLSYFFETPASATITMIFISIFLLVSLGGMLKKRLF"
     gene            complement(3883..4266)
                     /locus_tag="VGT87_000410"
     CDS             complement(3883..4266)
                     /locus_tag="VGT87_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEQ3813297.1"
                     /translation="MTECIFCHQLKANQLLTQSQYFKVVFDIDPIQKGHLLLISKDHY
                     TSLTQLPKEVRYDLIDLQAALVAKLEQHLPISGVTSVSNDKELMEEGTHFHLHLIPRL
                     TNDSFWESITINQENWDLTPFLKHL"
     gene            complement(4317..5573)
                     /locus_tag="VGT87_000411"
     CDS             complement(4317..5573)
                     /locus_tag="VGT87_000411"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEQ3813298.1"
                     /translation="MNIFEELKARGLVFQTTDEQALVKALTEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKETVLEWSDKIK
                     GQLSTFLDFENGDNKAELVNNYDWFSQISFIDFLRDVGKYFTVNYMMSKDSVKKRIET
                     GISYTEFAYQIMQGYDFYELNDKHNVTLQIGGSDQWGNMTAGTELLRKKADKTGHVMT
                     VPLITDSTGKKFGKSEGNAVWLDADKTSPYEMYQFWLNVMDDDAVRFLKIFTFLSLDE
                     IAEIETQFNAARHERLAQKTLAREVVTLVHGEEAYKQALNITEQLFAGNIKNLSANEL
                     KQGLSNVPNYHVQSIDNHNIVEILVAAKISPSKRQAREDVQNGAIYINGDRVQDLDYQ
                     LNNDDKIDDQLTVIRRGKKKYAVLTY"
     gene            5665..7977
                     /locus_tag="VGT87_000412"
     CDS             5665..7977
                     /locus_tag="VGT87_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEQ3813299.1"
                     /translation="MRYFMVKWNTKQKRISHQRLGLLDLGPVLLRTLRLLSNFFYIVI
                     FLFGMMGFGMAFGYLASQIESVKVPSKESLVKQVESLTMISQMNYSDNSLISTLDTDL
                     LRTPVANDAISENIKKAIVSTEDEHFQEHKGVVPKAVFRATLASVLGFGEASGGSTLT
                     QQLVKQQVLGDDPTFKRKSKEIVYALALERYMSKDNILCDYLNVSPFGRNNKGQNIAG
                     VEEAARGIFGVSAKDLTVPQAAFLAGLPQSPIVYSPYLSTGQLKSEKDMAYGIKRQQN
                     VLYNMYRTGVLSKKEYEDYKAYPIQKDFIQPGSAIVNNHDYLYYTVLADAKKAMYSYL
                     IKRDKVSSRDLKNDETKAAYEERALTELQQGGYTITTTINKPIYNAMQTAAAQFGGLL
                     DDGTGTVQMGNVLTDNATGAVLGFVGGRDYALNQNNHAFNTVRSPGSSIKPIIAYGPA
                     IDQGLMGSASVLSNYPTTYSSGQKIMHADSEGTAMMPLQEALNTSWNIPAFWTQKLLR
                     EKGVDVENYMTKMGYKIADYSIESLPLGGGIEVSVAQQTNAYQMLSNNGLYQKQYIVD
                     KITASDGTVVYKHENKPIRIFSAATATILQELLRGPITSGATTTFKNRLAAINPWLAN
                     ADWIGKTGTTENYTDVWLVLSTPKVTLGGWAGHDDNTSLAPLTGYNNNSNYLAYLANA
                     INQADPNVIGVGQRFNLDPGVIKANVLKSTGLQPGTVNVNGHTFSVGGEMTTSLWSQK
                     GPGAMTYRFAIGGTDADYQKAWGNFGFRKN"
     gene            8241..11807
                     /gene="rpoB"
                     /locus_tag="VGT87_000413"
     CDS             8241..11807
                     /gene="rpoB"
                     /locus_tag="VGT87_000413"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEQ3813300.1"
                     /translation="MAGHEVRYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFQDFLDSG
                     LKEVFEDVLPISNFTDTMELEFVGYEFKEPKYTLEEARIHDASYSAPIFVTFRLVNKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELETDSKDIAYTRIDRTRKIPFTTLVRALGFSGDDEIVDIFGESDLVR
                     NTIEKDIHKNPSDSRTDEALKEIYERLRPGEPKTADSSRSLLIARFFDARRYDLAAVG
                     RYKVNKKLNIKTRLLNQIIAENLVDAETGEILVEAGTEMTRSVIESIEEHLDGDLNKF
                     VYTPNDYAVVTEPVVLQKFKVVSPIDPDRVVTIVGNANPDDKVRALTPADILAEMSYF
                     LNLAEGLGKVDDIDHLGNRRIRAVGELLANQFRIGLARMERNVRERMSVQDNDVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSFGHLNKYGFIQTPYRKVDRATGRVTNEIV
                     WLTADEEDEYTVAQANSKLNEDGTFAEEIVMGRHQGNNQEFSASVVDFVDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLIDPKAPYVGTGMEYQAAHDSGAAVIAQH
                     NGKVVFSDAEKVEIRRQDGSLDVYHITKFRRSNSGTAYNQRTLVKVGDIVEKGDFIAD
                     GPSMENGEMALGQNPVVAYMTWEGYNFEDAVIMSERLVKEDVYTSVHLEEFESETRDT
                     KLGPEEITREIPNVGEEALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGGDGIVRDVKIFTRANGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARNLGIHIATPVFDGASSEDLWDTVREAGMDSDAKTVLYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDVTGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDN
                     EVELRDLDEGEDDDIMHVDDLEKAREKQAQETQEVSETTDEK"
     gene            11898..15521
                     /gene="rpoC"
                     /locus_tag="VGT87_000414"
     CDS             11898..15521
                     /gene="rpoC"
                     /locus_tag="VGT87_000414"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEQ3813301.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRAKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEPKSLLTEREYREKLQ
                     EYGHGSFVAKMGAEAIQDLLKRVDLAAEIAELKEELKSASGQKRIKAVRRLDVLDAFN
                     KSGNKPEWMVLNILPVIPPDLRPMVQLDGGRFAASDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVAKEYAGNVKAAKRMVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARLLMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEDA
                     GREGEGMIFKDKDEAVMAYRNGYAHLHSRVGIAVDSMPNKPWKDSQRHKIMVTTVGKI
                     LFNDIMPEDLPYLQEPNNANLTEGTPDKYFLEPGQDIQEVIDGLEINVPFKKKNLGNI
                     IAETFKRFRTTETSAFLDRLKDLGYYHSTLAGLTVGIADIPVIDNKAEIIDAAHHRVE
                     EINKAFRRGLMTDDDRYVAVTTTWREAKEALEKRLIETQDPKNPIVMMMDSGARGNIS
                     NFSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADS
                     GYLTRRLVDVAQDVIIREDDCGTDRGLLIRAITDGKEVTETLEERLQGRYTRKSVKHP
                     ETGEVLIGADQLITEDMARKIVDAGVEEVTIRSVFTCATRHGVCRHCYGINLATGDAV
                     EVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRIQEIFEARNPKGEAV
                     ITEVKGNVVEIEEDASTRTKKVYVQGKTGMGEYVVPFTARMKVEVGDEVNRGAALTEG
                     SIQPKRLLEVRDTLSVETYLLAEVQKVYRSQGVEIGDKHVEVMVRQMLRKVRVMDPGD
                     TDLLPGTLMDISDFTDANKDIVISGGIPATSRPVLMGITKASLETNSFLSAASFQETT
                     RVLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNIEPQAMNEIEVIDHTEVSA
                     EAE"
     gene            15673..16038
                     /locus_tag="VGT87_000415"
     CDS             15673..16038
                     /locus_tag="VGT87_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEQ3813302.1"
                     /translation="MYQVIKMYGDWEPWWFIDGWQDDIIDEQQFSDWQEALDYFNQEW
                     QRMKAIFPSYHSQKNLLATFWEKEDKRWCEDCDEDLQQFHSLLLLKNKDIVPSNNYIP
                     EFEQRNDSPQVAYLCKLNL"
     gene            16131..17069
                     /gene="tadA"
                     /locus_tag="VGT87_000416"
     CDS             16131..17069
                     /gene="tadA"
                     /locus_tag="VGT87_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEQ3813303.1"
                     /translation="MVQALAKAILAKAEQVQAQDIYILPRADQYDLFLRIGDERRLVD
                     VYQSDRMAPLISHFKFVAGMIVGEKRRCQVGSCDYKLSKDKQLSLRLSSVGDYRGQES
                     LVIRLLHHQNKSVHYWFDGLTKVANQVGGRGLYLFAGPVGSGKTTLMYQLISNYHQEA
                     QVISIEDPVEIKNHQILQLQVNDDIGMTYDNLIKLSLRHRPDILVIGEIRDSQTARAV
                     IRASLTGAMVFSTVHAKSISGVYVRLLELGVTKAELSNCLALIAYQRLLNGGALIDST
                     QNEFEYYSSSNWNQQIDQLLEAGHLNPKQAKLEKII"
     gene            17053..18040
                     /locus_tag="VGT87_000417"
                     /pseudo
     CDS             17053..18040
                     /locus_tag="VGT87_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
     gene            18042..18368
                     /locus_tag="VGT87_000418"
     CDS             18042..18368
                     /locus_tag="VGT87_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEQ3813304.1"
                     /translation="MINQWNNLRHKKLKGFTLLEMLLVILVISVLMLLFVPNLSKQKD
                     RVTETGNAAVVKLVENQAELYELSQGSKPSLSQLKADGSITEKQEKAYQDYYDKHKNE
                     KARLSN"
     gene            18343..18771
                     /locus_tag="VGT87_000419"
     CDS             18343..18771
                     /locus_tag="VGT87_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEQ3813305.1"
                     /translation="MKKPVLAIKAFTLLETLLSLSVMSFIILGLSVPVTKSYQKVEEH
                     LFFSHFEHLYRHQQKLAILQQKQRVLDISSTKIVTEGNSLTVPKSITVNHPYRLVIDQ
                     MGGNHSLAKIIFDMTDRRFKYQFYLGSGNYQKTSQSLHSP"
     gene            18776..19012
                     /locus_tag="VGT87_000420"
     CDS             18776..19012
                     /locus_tag="VGT87_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComG"
                     /protein_id="HEQ3813306.1"
                     /translation="MIATGILFSIVILVLSSLQQSQAALTYYRKQQEKLNLALMAVQT
                     RTKEMTLNGCHITILRTDRYISIHDDEGEVMKIE"
     gene            19005..19439
                     /locus_tag="VGT87_000421"
     CDS             19005..19439
                     /locus_tag="VGT87_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEQ3813307.1"
                     /translation="MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYL
                     ARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEKGKKVLAFGQFKSHDFRKSASNG
                     KGYQPMLFGISRSHIHIGQSQICITLKWKSGLERTFYYAFQD"
     gene            19423..19749
                     /locus_tag="VGT87_000422"
     CDS             19423..19749
                     /locus_tag="VGT87_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComG"
                     /protein_id="HEQ3813308.1"
                     /translation="MPFKTNLKAGILLYAMFMASIFLLVLQVYLSQVTALHKEYQAQT
                     DYVKARLIAEIVYQDHRYKASNPVFFKGGQVICRERKERWMLIVKLDQQRQYQFEYLK
                     PKQYSQ"
     gene            19847..20800
                     /locus_tag="VGT87_000423"
     CDS             19847..20800
                     /locus_tag="VGT87_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEQ3813309.1"
                     /translation="MTFEKIEEAYQLLLENCQLIENDLKTHIYDAIVEQNSFYLGAEG
                     ASPQVAQNSDKLKALCLTKEEWRKAYQFLFIKAAQTEQLQANHQFTPDAIGFILLYLL
                     EQLSDKDSLEVLEIGSGTGNLAQTLLNNTSKSLDYVGIELDDLLIDLSASIAEIMDSS
                     AHFIQEDAVRPQLLKESDIVISDLPVGYYPNDDIAKRYKVASSDKHTYAHHLLMEQSL
                     KYLKKDGFAIFLAPVNLLTSPQSQLLKQWLKDYAQVVALITLPDSIFGHPSNAKSIIV
                     LQKQTDHPMETFVYPIRDLKLAENIHDFMENFKKWKLSNVN"
     gene            20859..22055
                     /locus_tag="VGT87_000424"
     CDS             20859..22055
                     /locus_tag="VGT87_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEQ3813310.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEEEVLAQGIIERIGLKDSISTV
                     KYDGKKEEQILDIHDHTEAVKILLNDLIHFGIIAAYDEITGVGHRVVAGGELFKESVV
                     VNDKVLEQIEELSVLAPLHNPGAAAGIRAFRDILPDITSVCVFDTSFHTSMAKHTYLY
                     PIPQKYYTDYKVRKYGAHGTSHKYVAQEAAKMLGRPLEELKLITAHIGNGVSITANYH
                     GKSVDTSMGFTPLAGPMMGTRSGDIDPAIIPYLIEQDPELKDAADVVNMLNKKSGLSG
                     VSGISSDMRDIEAGLQEDNPDAVLAYNIFIDRIKKCIGQYFAVLNGADALVFTAGMGE
                     NAPLMRQDVIGGLTWFGMDIDPEKNVFGYRGEISTPESKVKVLVISTDEELCIARDVE
                     RLKNTK"
     gene            22242..22550
                     /locus_tag="VGT87_000425"
     CDS             22242..22550
                     /locus_tag="VGT87_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813311.1"
                     /translation="MYVFIEPNDLKDYLITVYDERNRAIRKQTQVYCFYLMIVLFVAL
                     FFFFPCLTIRDLVSYKLALIFAYLYLIIRSLVAKKMEALGKITTNSQKPPFDTSQVLC
                     "
     gene            complement(22633..23403)
                     /locus_tag="VGT87_000426"
     CDS             complement(22633..23403)
                     /locus_tag="VGT87_000426"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEQ3813312.1"
                     /translation="MKIGIIGIGKMASAIIKGLKQTPHELIISGSSLERSKEIAEQLA
                     LPYAMSHQDLIDQVDLVILGIKPQLFETVLKPLHFKQPIISMAAGISLQRLATFVGQD
                     LPLLRIMPNMNAQILQSSTALTGNALVSQELQARVRDLTDSFGSTFDISEKDFDTFTA
                     LAGSSPAYIYLFIEALAKAGVKNGMPKAKALEIVTQTVLASASNLKTSSQSPHDFIDA
                     ICSPGGTTIAGLMELERLGLTTTVSSAIDKTIDKAKSL"
     gene            complement(23451..24518)
                     /gene="pepA"
                     /locus_tag="VGT87_000427"
     CDS             complement(23451..24518)
                     /gene="pepA"
                     /locus_tag="VGT87_000427"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEQ3813313.1"
                     /translation="MTDLFSKIKEVTELDGIAGYEHSVRDYLRTKITPLVDRVETDGL
                     GGIFGIRDSKAEKAPRILVAAHMDEVGFMVSDIKVDGTLRVVGIGGWNPLVVSSQRFT
                     LYTRTGQVIPLISGSVPPHFLRGANGSASLPHIEDIVFDGGFTDKAEAERFGITPGDI
                     IVPQSETILTANQKNIISKAWDNRYGVLMITEMLEALKGQDLNNTLIAGANVQEEVGL
                     RGAHVSTTKFDPELFFAVDCSPAGDIYGNPGTIGDGTLLRFYDPGHVMLKDMRDFLLT
                     TAEEAGVNFQYYCGKGGTDAGAAHLQNGGVPSTTIGVCARYIHSHQTLYAMDDFVEAQ
                     AFLQAIIKKLDRSTVDLIKCY"
     gene            24974..25267
                     /locus_tag="VGT87_000428"
     CDS             24974..25267
                     /locus_tag="VGT87_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEQ3813314.1"
                     /translation="MRDMSKKKIGMISGIFGFSLAIGLGIVIKDYCQDRQRRQMTRDL
                     RTFFSPLGQIEVLYINPCQVKQDYISGGVVMSNGKQYQFTYHSRQISFEEDKG"
     gene            25264..25581
                     /locus_tag="VGT87_000429"
     CDS             25264..25581
                     /locus_tag="VGT87_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEQ3813315.1"
                     /translation="MIRPTSYESLAALIEKEDKLVLFFTADWCPDCQFIYPIMPEIEA
                     ELTDMTFVCVNRDQFIEVAQKWDIFGIPSFVVIEKGQEVGRLVNKMRKTKTEIMHFLA
                     AYQ"
     gene            25599..26225
                     /locus_tag="VGT87_000430"
     CDS             25599..26225
                     /locus_tag="VGT87_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEQ3813316.1"
                     /translation="MIFAYNKEQVGDVLMVILQDTKDIKRQVERKGKVARVFAEESGK
                     TLAWNIFEASSLITIEGNGQIFLTDEDLARLNAELAKEGFSERLEPTVGPVFVVGQIV
                     EMVAHPDSDHLNICQVAIGEDQTVQIVAGAPNAALGLKTIVALPGAIMPNGSLIFPGK
                     LRGEESYGMMCSPRELALPNAPQKRGIIELDESAVVGEAFDLAKHWKG"
     gene            26377..26772
                     /locus_tag="VGT87_000431"
     CDS             26377..26772
                     /locus_tag="VGT87_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEQ3813317.1"
                     /translation="MYNKVIAIGRLVAKPELVKTATDKHVARLSLAVNRRFKNASGER
                     EADFISVVVWGKLAETLVSYASKGSLMSIDGELRTRKYDKDGQVHYVTEVLCQSFQLL
                     ESRAQRAMRENNVTNDLVDLVLEEDTLPF"
     gene            complement(27032..27673)
                     /locus_tag="VGT87_000432"
     CDS             complement(27032..27673)
                     /locus_tag="VGT87_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEQ3813318.1"
                     /translation="MLIVLAGTIGAGKSSLAAALGEHLGTDVFYEAVDNNPVLDLYYQ
                     DPKKYAFLLQIYFLNKRFKSIKEAYQADNNILDRSIFEDELFLKLNYKNGNVTKTELD
                     IYQELLANMLEELEGMPKKRPDLLIYIDVSFDKMLERIEKRGRSFEQVDGNPSLEQYY
                     HQVHGEYPAWYEDYKVSPKMKIDGNSLDFVQNPQDLATVLKMIDTKLKELHLL"
     gene            complement(27693..28670)
                     /gene="dusB"
                     /locus_tag="VGT87_000433"
     CDS             complement(27693..28670)
                     /gene="dusB"
                     /locus_tag="VGT87_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEQ3813319.1"
                     /translation="MIKLNSSFRIGDVEIPHRTVLAPMAGVTNSAFRTIAKEFGAGLV
                     VMEMISEKGLLYNNEKTLHMLHIDENEHPMSIQLFGGDAEGLKRAADFIQTNTKADIV
                     DINMGCPVNKVVKNEAGAKWLRDPDKIYHIVKEVTSVLDIPLTVKMRTGWADSSLAVE
                     NALAAESAGVSALAMHGRTREQMYTGTCDHETLARVSKAITKIPFIGNGDVRSVQDAK
                     FMIEEIGVDAVMIGRAAMNNPYLFTQINHFFETGQELADLPFAKKLDIAKDHLKRLIN
                     LKGETIAVREFRGLAPHYLRGTAGAAKVRGAVSRAETLAEVEAIFETVR"
     gene            complement(28657..29529)
                     /gene="hslO"
                     /locus_tag="VGT87_000434"
     CDS             complement(28657..29529)
                     /gene="hslO"
                     /locus_tag="VGT87_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEQ3813320.1"
                     /translation="MDKIIKSIAQSGAFRAYVLDSTETVALAQEKHNTLSSSTVALGR
                     TLIANQILAANQKGDSKITVKVIGDSSFGHIISVADTKGHVKGYIQNTGVDIKKTATG
                     EVLVGPFMGNGHFVTIIDYGTGNPYTSTTPLITGEIGEDFAYYLTESEQTPSAIGLNV
                     LLDENDKVKVAGGFMVQVLPGASEEEIARYEKRLQEMPAISHLLASKNHVDSLLEAIY
                     GDEPYKRLSEEPLSFQCDCSRERFEAALMTLPKADLQAMIDEDKGAEIVCQFCGTKYQ
                     FNESDLEALINDKA"
     gene            complement(29676..>30495)
                     /gene="rofA"
                     /locus_tag="VGT87_000435"
     CDS             complement(29676..>30495)
                     /gene="rofA"
                     /locus_tag="VGT87_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047149466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator RofA"
                     /protein_id="HEQ3813321.1"
                     /translation="KKLFVYDSLKKSSHDIIETYCQLNFSAGDLDYLYLIYITANNSF
                     ASLQWTPEHIRQCCQLFEENDTFRLLLNPIITLLPNLKEQKASLVKALMFFSKSFLFN
                     LQHFIPETNLFVSPYYKGNQKLYTSLKLIVEEWMAKLPGKRDLNHKHFHLFCHYVEQI
                     LRNIQPPLVVVFVASNFINAHLLTDSFPRYFSDKSIDFHSYYLLQDNVYQIPDLKPDL
                     VITHSQLIPFVQHELTKGIAVAEISFDESILSIQELMYQVKEEKFQADLTKQLT"
BASE COUNT         9107 a         5516 c         6625 g         9247 t
ORIGIN      
        1 taaattttat atttaaagcc ggcgtggcgg aattggcaga cgcgctggac tcaaaatcca
       61 gtgtccgcaa ggacgtgccg gttcgacccc ggccgccggt atacttgaaa aacaaggttt
      121 gtaaaccttg ttttttgttt tgatcttagg ctgtagctac gtagtacaat ctcttatatt
      181 actgataagg tctatttaat gcgcaagatt tgtggtatga tgaatatgat tgagtattga
      241 tgctttatgg gattaccggt aaggttttta aaaaggtttg tcactactag gtgctttttt
      301 aactatagaa agacagttat ggattgatgt attgtaacta atgttggagg ttaatggtgg
      361 ttgagttttg agatatttac actgataatc gggtggaaac tggtcgggta tcgggtaatg
      421 gagtgtggtt cttcgtttaa gcaaggagat tatcgtttgg tggttcatat ttgcttgtta
      481 aatgttaggg atgagatgct gagtcagcaa ggccatgaag ataaagctga ctggctagag
      541 ccttgagatg tgacgtttgg tggaagtcct ttttggtgag atggcacagc atatagtaat
      601 attagatcct gaggaagaat tgggactttc attaggcttg actggggtat gacctcactt
      661 tacaattcat ttcgatgatg ggtttgatga tacgttttta gttcaagact ttgtagagtt
      721 ggagaatttg gttctttatg aggaagaagt gcaggtaatt cgttgagcca gtcgtgaaga
      781 tattttaggt atgattgatg aaggttcgtt cattccttat ctgaagtata aaataaattt
      841 gtgctttgag atggttggtc aatataaagt ccatcaatct tagaagtaat aatttttgaa
      901 ttttttctga ggtttgtgta tatttgatag tgtactgatg taagaagtaa ggagtactat
      961 gatggcaatc aatgagagag cttctgcaaa aatcaagtta ggccttgata ctaagaataa
     1021 gcgatgagat ggttaccacg atttatcgaa ggttatgatg agtattgatc tgtgtgacta
     1081 tgtgacggtt gaccatatag atgacaataa gattgtcttt gcctcaaact gtccgaaaat
     1141 acctattaac taagataatg atgtttataa gatagttcag ctaatgaaac atcgatttca
     1201 agttaaaaga ggagtttccg tttacttgga aaagcggatt cccatgtgtg ctggtatggg
     1261 aggaggttct agcgatgctg ttactatcag agcattaaat cagttatggc tccttactct
     1321 tagccgaaaa gatatgatgg atattggtat accgattggt agtgctgtac cttattgctt
     1381 gctttcaggt tgtgcccaag ttactggtaa aggtgaagtg gtttgtcgca ttttggggct
     1441 gttatcgtct tgggtggttt tggttaaacc agattttggt atctcaactt gaacaatttt
     1501 tctagatatt aattgtaaaa ccatttcaag agtgtctact actcatttgg ttgcggcgat
     1561 tgaagcgggt aactataatg atgggatatt gactgaaatg aacaatttgc tggaagatat
     1621 tttcattgcc aagcgaccct ttattcaaaa aataaaagag aaaacactgc aagctggtgc
     1681 tgctactact ttgatgacag gaagtggacc gactgttttt gctttgtgcc agaccgaaaa
     1741 acaaactata atagtttaaa aggactttgc aaggaagttt ttaaggtgag aatgttgtaa
     1801 tattttgttg ggattgcttc tttggaagct tcatgttata attaactggt taatgaaaga
     1861 ggagatttat ggggacttta gaaaaaaaac ttgataactt agtaaatact attttattaa
     1921 aagcagaaaa tcagcatgag ttattatttg gagcttgtca aagtgacgtt aagcttacta
     1981 atacgcaaga acatatttta atgttactat ctcagcaacg tctcactaat acagatttgg
     2041 ctaaggtatt aaatattagt caggcggcag taactaaggc tatcaagagt ttggtcaaac
     2101 aagacatgct agcaggaact aaggatacgg ttgatgctag ggtgacttat tttgaattaa
     2161 ccgagttagc taagcctatt gcatcagaac atacccatca tcatgatgaa actttaaatg
     2221 tttacaaccg tttattacaa aaattctctg cgaaagaatt agagattgta gataagtttg
     2281 taacagtttt tgctgaggaa ttagaagggt aatccatgag atacatatca gtgaaaaatc
     2341 tctcctttca atatgaaagt gagccagttt tagaagggat cacttatcat ttagatagtg
     2401 gagaatttgt caccatgacc ggtgaaaatg gcgctgcaaa gtcaacctta ataaaagcaa
     2461 ccttaggaat tttacaacca aaggctggac gagttactat tgctaaaaaa aataaagacg
     2521 gtaaacaatt aaggattgct tacttgccgc agcaagtagc tagctttaac gctggttttc
     2581 catccaccgt ttacgagttt gtcaaatcag gtcgctaccc acgtagtggt tggtttagac
     2641 atttgaacaa acacgatgaa gagcatgtac aagcaagctt agaagcagtc ggcatgtggg
     2701 aaaaccgtca taagagaatt ggtagtttat caggtggtca aaaacaacgt gtggttattg
     2761 cccgtatgtt tgcttctgac cctgatattt ttgtgctaga cgagccaaca acgggaatgg
     2821 atagcggtac tactgatacc ttttatgaac tgatgcacca cagtgcacat caacatggga
     2881 aatccgttct gatgattacc catgacccag aagaagtgaa ggcttatgct gatcggaaca
     2941 ttcatttagt cagaaaccaa aaacttcctt ggcgttgttt caacattcat gaagctgaaa
     3001 cagatgacga aaaaggaggt catggtcatg cttgatattt tatcctatga tttcatgcaa
     3061 cgggcggtaa tggcggtagt tgccattagt atttttgctc cgattttagg tattttcctt
     3121 attttacgtc gtcaaagttt gatgagcgat acccttagtc atgtttcttt ggctggggta
     3181 gcgcttgggg tagtcattgg tatttcacca accatcacta ctattattgt tgtggtttta
     3241 gctgctattt tgttagaata cctgcgtgta gtttacaaac actacatgga gatttcaacg
     3301 gcgattttga tgtcacttgg cttggcccta tctctgatta ttatgagtaa gtcgcatagt
     3361 tcatcaagca tgagtttaga acaatacctt tttggatcga tcatcacgat tagtatggaa
     3421 caagttgtcg ccttgtttgc tattgctgcg attattttaa tcttgaccgt tctcttcatt
     3481 agaccgatgt acattctgac ctttgatgaa gatactgctt ttgtagatgg tctgcccgtt
     3541 cgcttgatgt ctgttctatt caatatcgtc actggggttg ctattgcttt gaccattcca
     3601 gcagcaggag cacttttggt ttctaccatt atggtcttgc cagcaagtat cgcaatgaga
     3661 ttgggtaaaa actttaaaac agttatctta ctgggaattg tcatcggttt tagcggtatg
     3721 ttatctggta ttttcttatc ttatttcttt gaaacgccag ctagtgccac tattaccatg
     3781 attttcatta gtattttcct cttagttagt ctaggtggaa tgcttaaaaa acggttattt
     3841 taataagaaa aatcctcaca tgtttaacat gtgaggattt atttataaat gctttaaaaa
     3901 gggggtaagg tcccaatttt cttgattaat ggtgatgctc tcccaaaagc tatcattagt
     3961 taaacgagga atcaaatgaa ggtgaaaatg agtcccctcc tccatcaact ccttgtcatt
     4021 gctgacgcta gtgacgcctg aaatgggaag gtgctgctct agcttagcaa ctagagcggc
     4081 ttgtaagtcg atgagatcat agcgcacttc ctttggtagt tgtgttagac tagtgtaatg
     4141 gtctttggaa atcagtaaaa ggtgcccctt ttggatagga tcaatatcaa aaacaacctt
     4201 gaaatattga ctttgagtca gtagctgatt tgctttgagc tgatggcaaa agatacattc
     4261 tgtcataggt acatctcctt atgaaaaaac gagccagagc tcgtttaatc ggttatctag
     4321 taagtgagaa cagcgtattt tttcttaccg cggcgaataa cggttaattg atcatcaatt
     4381 ttatcatcat tatttaattg gtaatctaaa tcttgaacgc ggtctccgtt aatgtagatt
     4441 gctccatttt gcacgtcttc acgcgcctgg cgttttgatg gggaaatctt agcagctact
     4501 aaaatctcga caatattatg attgtctatt gattgaacgt gataatttgg tacattgctc
     4561 aatccttgtt ttaattcatt agcagaaaga tttttgatgt ttccagcaaa taactgctcg
     4621 gtaatgttaa gagcttgttt ataagcttct tcgccatgaa ccaaggtaac cacttcgcgt
     4681 gccaaggttt tttgagcgag acgctcgtga cgagcggcat taaattgagt ttcaatttct
     4741 gcaatctcat ctagagataa gaaggtgaag attttcaaga aacgcactgc atcatcatcc
     4801 atgacattta accagaattg gtacatctcg taaggagacg tcttatcggc atcaagccag
     4861 acggcattac cttctgattt accgaatttt ttcccggttg agtcagtgat gagtggtaca
     4921 gtcatgacgt gcccagtttt atcagccttt ttgcggagca attcagtacc agctgtcata
     4981 ttgccccact ggtcagagcc gccaatttgt aaggttacat tatgcttgtc attgagttcg
     5041 tagaagtcgt agccctgcat gatttggtaa gcaaactcag tgtaagaaat gcctgtttca
     5101 atgcgttttt taacagagtc tttactcatc atgtagttaa cggtgaagta tttaccgaca
     5161 tcacggagga agtcaataaa gctgatttgc gagaaccagt cgtagttgtt gacaagctct
     5221 gctttattat caccattttc aaaatcaagg aaagtagaca actgcccttt aatcttgtca
     5281 ctccactcca aaactgtttc tttggtttga aggctgcgct ctgcatcttt aaaggaagga
     5341 tcaccaatta aacctgtggc accacccaca agagcgtaag gtttgtgccc tgctagttgc
     5401 aagcgacgag atgttaagat agccactaga tggcccagat gaaggctgtc agcggttgga
     5461 tcataaccgg tataatagga tacttgccct tctgttaatg ctttgacaag ggcttgttca
     5521 tcagttgttt gaaagaccaa gccacgagct ttgagttctt caaaaatatt cataggcttt
     5581 ctccattttc attatagttt cgaatcatta tatcaaaaaa cgctactgct atgcaagctt
     5641 tatcaaagtt tggtataatg ataaatgagg tattttatgg tgaaatggaa cacgaaacaa
     5701 aagcgtataa gtcatcaaag attaggtctc ttggatttgg ggccggtgct attacgtacg
     5761 ttgagactac tgtctaactt tttttatatt gttatcttcc tttttggaat gatgggattt
     5821 ggtatggcat ttgggtattt ggctagtcag attgaatctg ttaaggtacc aagtaaagaa
     5881 agtttagtca aacaagttga atcattaacg atgatttcgc aaatgaacta ttctgataat
     5941 agtttaattt ctactttaga tacggattta cttcgaacac cagtagctaa tgatgcgatt
     6001 tcagagaata tcaaaaaagc tattgtatca acagaagacg aacattttca agaacataaa
     6061 ggtgtcgtgc caaaagctgt ttttcgggca acattggctt ctgtattggg atttggagaa
     6121 gctagtggag gttcgacctt aacacagcaa ttagtcaagc aacaagtttt gggagacgat
     6181 cccacattta agcgcaagtc taaggagatc gtttacgctc ttgccttaga gcgttatatg
     6241 tccaaagaca atatcttatg tgattatctt aatgtttcac cttttggccg taacaacaag
     6301 ggtcaaaata ttgctggtgt tgaagaagct gcgcgtggca tttttggcgt ttctgccaaa
     6361 gatttaacgg tgccacaggc agcatttttg gcgggtcttc cgcagagtcc tattgtttac
     6421 tctccttatt tgtcaacggg acaactgaag tcagaaaagg acatggctta tggcatcaag
     6481 cgtcagcaaa atgttctcta taacatgtac cgtacaggtg ttctgtctaa aaaagaatac
     6541 gaggactata aggcttatcc gattcaaaag gattttattc aaccgggaag tgcaatagta
     6601 aataatcacg attaccttta ttacacggtg ttagcggatg ctaagaaagc catgtatagc
     6661 tatttgatta agcgagataa ggtgtctagt cgtgacttga aaaatgacga gactaaggct
     6721 gcttatgaag agagagcctt aacagaattg caacagggtg gctataccat caccacaacc
     6781 attaataagc ctatttacaa tgcgatgcag acagcggcag ctcagtttgg tggtttgtta
     6841 gatgatggca ctggtacagt tcaaatggga aatgtcttga cagacaatgc gactggtgct
     6901 gtgttaggtt ttgttggtgg tagagattat gctctgaatc aaaataatca tgctttcaat
     6961 acagttagat cgccaggttc tagcattaaa ccgataatcg cttatggtcc tgctattgat
     7021 caaggtttaa tggggagtgc tagcgttttg tctaattacc caacaactta ctcgagtggt
     7081 caaaaaatca tgcatgctga tagtgaagga acagccatga tgccacttca agaggcccta
     7141 aatacttctt ggaacatccc agctttttgg acacagaaat tactgcgtga aaaaggggtc
     7201 gatgtcgaaa attatatgac aaaaatgggt tataagattg cagactactc gattgaaagt
     7261 ttacctctag ggggcggtat tgaagtctcg gttgctcaac aaaccaatgc ttaccaaatg
     7321 ctttcaaaca atggcttata tcaaaagcaa tatattgtag ataagattac tgctagcgat
     7381 ggtacagtcg tttacaaaca tgaaaataag ccaattcgta ttttttctgc agcaacagct
     7441 acgattttac aagaattgtt gagaggtccg attacttcag gcgctacgac tactttcaag
     7501 aaccgtttgg cggctattaa tccgtggctt gctaatgctg attggattgg taagaccgga
     7561 acaactgaga attatacgga tgtttggcta gtcctgtcta ctccaaaagt taccttaggc
     7621 ggttgggcag gacatgatga caatacctca ttagcgccat taacaggata taacaataat
     7681 tctaattacc ttgcctattt agctaatgcc attaatcagg ccgatcccaa tgttattgga
     7741 gtagggcaac gcttcaactt agatccagga gtcattaagg cgaatgtctt gaagtcaaca
     7801 ggtttacaac caggaactgt taatgtcaat ggacatactt tttctgttgg tggagaaatg
     7861 accaccagtc tatggtccca aaaaggaccg ggggctatga cttaccgatt tgctattggt
     7921 ggcacggatg ccgattatca aaaagcctgg gggaactttg ggttcagaaa aaattaggtt
     7981 gccatttatc gataaaaagg gtataatagc aataactatt tgtcgtccaa tctagctgaa
     8041 atattgtcca gttaggaaga acagcagtta aatcacactg ataaagtcag atttagctgc
     8101 tctttttgtg tctattttta gaaaagatag ggtttgtaac ctatatttaa atattctaaa
     8161 aattaacatt tagggcaaat gataacttag ttgcgatttg ctgaaacagc aaagcttaaa
     8221 tagaagaagg agctaaaaac ttggcaggac atgaagttcg atacggaaaa caccgtacac
     8281 gtcgtagctt ttcaagaatc aaagaagttc ttgatttacc aaatttgatt gaaattcaaa
     8341 ctgactcatt ccaagatttc ctcgattcag gtttgaaaga agtatttgaa gatgtacttc
     8401 ctatttcaaa ctttacggat actatggaac ttgaatttgt tggttacgaa tttaaagaac
     8461 ctaaatatac ccttgaagaa gctcgtatcc acgatgcaag ttattctgca ccaatctttg
     8521 ttaccttccg tttggtcaat aaagaaactg gtgagattaa aactcaagaa gtcttctttg
     8581 gtgatttccc aatcatgacg gaaatgggta cctttattat caatggtggt gaacgtatca
     8641 tcgtttctca gttggtgcgt tctccaggtg tttatttcaa cgataaagtt gataaaaacg
     8701 gtaaagttgg ttacggatca acagtgattc ctaaccgtgg ggcttggttg gaattagaga
     8761 ctgactcaaa agacattgcc tacactcgta ttgaccgtac ccgtaagatt ccattcacaa
     8821 ccttggttcg tgcccttggt ttctcaggtg atgatgaaat tgttgatatc tttggtgaaa
     8881 gcgaccttgt tcgtaatacc attgaaaaag acattcacaa aaatccaagt gattctcgta
     8941 cagacgaagc ccttaaagaa atttacgagc gtcttcgtcc aggtgagcca aaaactgcag
     9001 atagctctcg tagtctcttg attgcgcgtt tctttgatgc acgtcgttac gatttggctg
     9061 cggttggtcg ttacaaagtg aacaaaaaac ttaatatcaa gactcgtctt ttgaaccaaa
     9121 tcattgctga aaaccttgtg gatgctgaaa ctggcgaaat tttggtagaa gctggaactg
     9181 agatgactcg tagcgtcatc gaatccatcg aagaacacct tgacggtgat ttgaacaagt
     9241 ttgtttacac accaaatgat tacgctgtgg ttactgaacc tgttgttctt caaaagttca
     9301 aggttgtgtc accaattgat cctgatcgtg tagtaactat tgttggtaat gccaatccag
     9361 atgataaggt tcgtgcattg acacctgctg acattttggc agaaatgtct tacttcttga
     9421 accttgctga aggtcttgga aaagttgacg atattgacca cttgggtaac cgtcgtatcc
     9481 gtgccgttgg tgaattgctt gccaaccaat tccgcatcgg tcttgctcgt atggagcgta
     9541 acgttcgtga gcgtatgtct gttcaagaca acgatgtgtt aacaccacaa caaatcatca
     9601 atatccgtcc tgtcacagca gctgtcaaag aattcttcgg ttcgtctcag ttgtcacagt
     9661 tcatggacca acacaaccca ttgtcagagt tgtctcacaa acgtcgttta tctgccttag
     9721 gacctggtgg tttgacacgt gaccgtgctg gttatgaggt tcgtgacgtg cattacacgc
     9781 actatggccg tatgtgtccg attgaaacac ctgaaggacc aaacattgga ttgattaaca
     9841 acttgtcttc atttggacat cttaataaat atggtttcat ccaaacacct taccgtaagg
     9901 ttgaccgtgc gactggtagg gtaactaacg aaattgtttg gttgactgcc gacgaagaag
     9961 acgaatacac agttgcacag gccaattcga aactaaacga agatggcact tttgctgaag
    10021 aaatcgttat gggtcgtcac caaggtaata accaagaatt ttctgcaagt gttgttgatt
    10081 tcgttgacgt ttcccctaaa caggtagttg ctgttgcgac ggcatgtatt cctttcttgg
    10141 aaaacgatga ctccaaccgt gccctcatgg gtgccaacat gcaacgtcag gctgtgccat
    10201 tgattgatcc aaaagcacca tatgttggta ctggtatgga atatcaagct gcccatgact
    10261 caggcgctgc ggtgattgct cagcacaatg gtaaagttgt cttttctgat gctgaaaaag
    10321 tggaaatccg tcgtcaagat ggctcacttg atgtttacca cattaccaaa ttccgtcgtt
    10381 caaactcagg aacagcctat aaccaacgca cccttgttaa agtaggagac atcgttgaaa
    10441 aaggtgactt catcgctgat ggaccatcta tggaaaacgg tgaaatggct cttggacaaa
    10501 acccagtcgt tgcttacatg acttgggaag gttacaactt cgaggacgct gttatcatgt
    10561 ctgaacgcct tgtgaaagag gatgtttaca catctgttca cttggaagaa ttcgaatctg
    10621 aaacgcgtga tacaaaactt ggccctgaag aaatcactcg ggaaatccca aatgttggtg
    10681 aagaagccct caaagacctt gacgaaatgg gcattatccg tatcggtgct gaggtgaaag
    10741 aaggcgacat cttagtaggt aaagtcacac ctaaaggtga aaaagacctt tctgctgaag
    10801 aacgtttact tcacgccatc tttggggata aatcgcgtga agtgcgtgat acctcacttc
    10861 gtgtccctca cggtggtgat ggtatcgttc gtgatgtgaa aatctttaca cgcgctaacg
    10921 gcgatgaatt gcaatcaggt gttaatatgc ttgtgcgtgt ttacatcgct caaaaacgta
    10981 aaatcaaggt cggagataaa atggccggtc gtcacggaaa caagggtgtc gtttcacgta
    11041 ttgtaccagt tgaagacatg ccataccttc cagacggaac accagttgac atcatgttga
    11101 accctcttgg ggtgccatca cggatgaata ttggtcaggt tatggaactt caccttggta
    11161 tggctgctcg taatcttggc attcacattg caacacctgt ctttgacggg gcttcatcag
    11221 aagacctttg ggacactgtt cgcgaagctg gtatggatag cgatgctaag acggtccttt
    11281 atgatggtcg caccggtgaa ccatttgaca accgtgtatc cgttggtgtc atgtatatga
    11341 tcaaacttca ccacatggtt gatgataaac ttcatgcccg ttctgtagga ccatactcac
    11401 ttgttaccca acagccactt ggtggtaaag ctcaatttgg tggacaacgt tttggtgaga
    11461 tggaggtttg ggcccttgaa gcttatggtg catcaaatgt tcttcaagaa atcttgacct
    11521 acaagtcaga tgacgtcaca ggacgtttga aagcctatga agcgattact aaaggtaaac
    11581 caattccaaa accaggtgta ccagaatcct tccgtgttct tgtaaaagaa ttgcaatcgc
    11641 ttggtcttga tatgcgtgtg cttgatgagg atgataatga agtggaactt cgtgatcttg
    11701 atgaaggtga agacgatgac attatgcatg ttgacgatct cgagaaggca cgtgaaaaac
    11761 aagctcaaga aacccaagaa gtttctgaaa caactgacga aaaataagca atcaattctt
    11821 attaaataat tatttactgg tctggggcaa aggtcccagg aactggtaaa gtcatcaaag
    11881 gcagaaaggt aaaactagtg gttgacgtaa atcgttttaa aagtatgcaa atcacattag
    11941 cctcaccaag taaggtccgt tcatggtctt atggtgaagt taaaaaacct gaaacaatca
    12001 actaccgtac attaaaacca gaacgtgaag gactctttga cgaagtcatc tttggtccaa
    12061 caaaagactg ggaatgtgcg tgtggtaagt acaaacgtat ccgttataaa gggatcgttt
    12121 gtgaccgctg tggggttgag gtaactcgtg ctaaagtacg tcgtgaacgt atgggtcaca
    12181 ttgagttaaa agctcctgta tcacatatct ggtatttcaa agggattcca tctcgtatgg
    12241 gattgactct tgatatgagt cctcgtgccc ttgaagaagt gatctatttt gcggcttatg
    12301 tggtcattga tccaaaagat acaccgcttg aaccaaaatc attattaaca gaacgtgaat
    12361 accgtgaaaa actacaagag tatggtcatg ggtcatttgt cgctaagatg ggtgctgaag
    12421 ccattcaaga tctcttgaaa cgtgtggact tggcagctga aatcgctgaa ttaaaagaag
    12481 agttgaaatc tgcttctggt caaaaacgga ttaaagcagt tcgtcgttta gacgtccttg
    12541 atgcctttaa caaatctgga aataaaccag aatggatggt tcttaacatc ctgccggtta
    12601 ttccaccaga tcttcgtccg atggttcaat tggatggtgg ccgttttgcg gcatcagact
    12661 tgaatgactt gtaccgtcgt gtgattaacc gtaacaaccg tttggcacgt ttgttagaac
    12721 ttaatgcccc tggcatcatt gttcaaaatg aaaaacgaat gcttcaagaa gccgttgatg
    12781 ctttgattga taatggtcgt cgtggtcgtc caatcactgg accaggaagt cgtccattga
    12841 aatcattgag ccacatgctt aaaggtaaac aagggcgttt ccgtcaaaac ttacttggta
    12901 aacgagtaga cttctctgga cgttccgtta tcgctgttgg tccaaccctt aaaatgtatc
    12961 aatgtggtgt cccacgtgaa atggctatcg agctttttaa accatttgta atgcgcgaaa
    13021 ttgttgccaa agaatatgct ggtaacgtta aagccgctaa acgtatggtt gaacgtggcg
    13081 acgaacgcat ctgggatatc ttagaagaag ttatcaaaga acacccagtc ctacttaacc
    13141 gcgcaccgac ccttcacaga cttggtattc aggcttttga accggttctt attgacggta
    13201 aggcacttcg tcttcaccca cttgtgtgtg aggcctacaa tgccgacttc gatggagacc
    13261 aaatggccat ccacgtgcca ctttcagaag aagcacaagc tgaagctcgt cttttgatgc
    13321 ttgctgcaga gcacatcttg aaccctaaag atggtaaacc agttgttacc ccatctcagg
    13381 atatggttct tggtaactac tatcttacga tggaagatgc tggtcgcgaa ggcgaaggta
    13441 tgattttcaa ggataaagac gaagctgtga tggcatatcg taatggctat gctcatcttc
    13501 atagtcgtgt gggtatcgct gttgacagca tgccaaacaa accttggaaa gacagtcaaa
    13561 gacataaaat catggtgaca actgttggta agattctctt taatgacatc atgccagagg
    13621 accttcctta cctccaagag ccaaacaatg ccaacttgac agaaggaaca cctgataaat
    13681 acttccttga acctggtcaa gatatccaag aagtgattga cggcttagaa atcaatgtgc
    13741 cattcaagaa gaaaaacctc ggtaacatca ttgcggaaac cttcaaacgt ttccgtacaa
    13801 cagaaacatc agccttcctt gaccgtttga aagaccttgg ttactaccat tcaacccttg
    13861 ctggtttgac agtgggtatc gctgacattc ctgttattga taataaagct gaaatcattg
    13921 atgctgctca ccatcgtgtt gaagaaatta acaaagcctt ccgtcgtggt ttgatgacag
    13981 atgatgaccg ttatgttgcc gttacaacaa catggcgtga agctaaagaa gcccttgaaa
    14041 aacgtctgat tgaaacacaa gatcctaaga acccaatcgt tatgatgatg gactcaggag
    14101 ctcgtggtaa catctctaac ttctcacagc ttgcgggtat gcgtggtttg atggctgctc
    14161 ctaacggacg catcatggaa cttcctatct tgtcaaactt ccgtgaaggt ttgagcgttt
    14221 tggaaatgtt cttctcaacc cacggtgcac gtaaagggat gaccgatacg gcccttaaaa
    14281 cagccgactc aggttatctt actcgtcgtt tggttgacgt tgcccaagat gttatcattc
    14341 gtgaggacga ttgtggcact gatcgtggtc ttcttatccg tgctattaca gatggtaaag
    14401 aagttaccga aacgcttgaa gagcgtcttc aaggtcgtta cacacgtaaa tcagtcaaac
    14461 accctgaaac tggtgaagtc ttgattggtg ctgaccaatt aatcactgaa gacatggctc
    14521 gtaagattgt tgatgcaggt gttgaagaag tgaccattcg ttctgtcttt acctgtgcga
    14581 ctcgtcatgg tgtctgccgt cactgttatg gtatcaactt ggcaactggt gatgctgttg
    14641 aagtgggtga agcagttggt actattgctg cccaatctat cggtgagcct ggtactcagc
    14701 ttaccatgcg taccttccac acgggtggtg tagcctcaaa taccgatatc acccagggtc
    14761 ttcctcgtat tcaagagatc tttgaagcac gtaatcctaa aggggaagcg gtcattactg
    14821 aagtgaaagg gaatgtcgtc gagattgaag aagatgcgtc aactcgtacc aagaaagtct
    14881 acgttcaagg aaaaactggc atgggcgaat atgtcgtacc atttacagca cgtatgaaag
    14941 ttgaagttgg cgacgaagtt aatcgcggag ctgcccttac agaagggtca attcaaccga
    15001 aacgtctcct tgaagtgcgt gataccttgt cagttgaaac gtaccttctt gcagaagtac
    15061 aaaaagttta ccgtagccaa ggggtagaaa tcggagacaa acacgttgag gtaatggttc
    15121 gccaaatgct tcgtaaagtt cgtgtcatgg atccaggtga tacagacctc cttccaggta
    15181 cacttatgga tatttctgat ttcacagatg ctaacaaaga tattgttata tctggtggta
    15241 ttcctgcgac atctcgtcca gttcttatgg gtattactaa ggcttccctt gaaaccaatt
    15301 ccttcttatc agctgcatcc ttccaagaaa caactcgtgt tcttacagat gctgctatcc
    15361 gtggtaagaa agaccactta cttggtctta aagaaaatgt tatcattggt aaaatcatcc
    15421 cagctggtac tggtatggcg cgctaccgta acattgaacc acaagcgatg aatgagattg
    15481 aagtgattga tcatacagaa gtctcagcag aagcagaata atctcatgaa agagtcctta
    15541 ggggctcttt tttgtctgag tttgttggca gtaaaaagca ttgacaatca gtcttagtcc
    15601 caatgagcag atgtttttca aaatacttat gatttagcta taataatgca taagaggaat
    15661 gggtaatgtg ttatgtatca agtaattaaa atgtatggtg attgggagcc ttggtggttc
    15721 atcgatggat ggcaagacga tattatagat gagcaacaat ttagtgactg gcaagaagcg
    15781 cttgattatt ttaatcaaga atggcagcgc atgaaagcta tttttcctag ttatcatagc
    15841 caaaagaatt tgttagctac tttttgggaa aaagaagata aaagatggtg cgaggactgt
    15901 gacgaagatt tgcagcagtt tcattctctt ttactcctta aaaataaaga tattgtacca
    15961 agcaataatt atattcctga atttgaacaa cgaaatgatt caccacaggt agcttatctt
    16021 tgcaagttaa acctttaagt gtttgttaaa aacaaaataa gttaaaagaa aatatcactt
    16081 cttatgatat tttctttttt tgtttacgaa taaatgagta aggaggaatt atggtacaag
    16141 cattagcaaa agctattcta gcaaaagctg aacaggttca ggcacaagat atttatattt
    16201 tgccaagagc agatcaatat gatctttttt tacgaatagg agatgaaagg agattagttg
    16261 atgtttatca gagcgatcgg atggctcctc ttattagtca ctttaaattc gttgcaggaa
    16321 tgatagttgg tgaaaagaga cgttgtcagg tgggttcatg tgattataag cttagtaaag
    16381 ataagcagtt atctttgcgc ttatctagcg tgggtgatta tcgcgggcaa gaaagcttag
    16441 tgattcgtct gcttcatcat caaaataaaa gtgtacatta ttggtttgat ggattgacaa
    16501 aagtagccaa tcaagttggc ggtagagggt tgtatttatt tgcaggacca gttgggtctg
    16561 ggaagacaac cttgatgtac cagctgattt cgaattatca tcaagaagca caggttatta
    16621 gtatagaaga tcctgtagaa attaaaaatc accagatttt acaattacaa gtgaatgatg
    16681 atattggtat gacttatgac aatttgatca aactgtcttt acgccatcga ccagatattt
    16741 tagttattgg tgagattcga gatagtcaaa cagcaagagc cgttattagg gctagtctaa
    16801 caggtgccat ggttttttca acggttcacg ctaaaagtat ctcgggtgtt tatgtaagat
    16861 tgttagaact tggtgtaacg aaagcagaac tgtctaattg cttagcatta attgcttacc
    16921 aaaggttact taatggagga gcattgattg actctactca aaacgaattt gaatattatt
    16981 cctcatcgaa ctggaatcaa caaattgatc agcttcttga ggcaggacat ctcaatccca
    17041 agcaagctaa gcttgaaaaa attatctagt aaacatcagc ataaattcat tcaattacta
    17101 gctaatcttt tatctacagg atttagcttc gcagaggtta ttgctttttt aaaaagaagc
    17161 cagttgcttc agctagatta tgttcttaaa atggaagagt ctttattaaa aggacaaggc
    17221 ttagcagata tgttgtcggg tttaggtttt tcagatgcta ttcttactca aattagttta
    17281 gctgatagac acggtaatat tgaaacaaca ttagtagcga ttcaacatta cttgaaccaa
    17341 atggcaagga tcagacgaaa aactgttgaa gttatcacct atcctcttat tcttttactt
    17401 tttctttttg tgatgatgct agggttgaga cgctatttaa tccctcaatt agaaactcaa
    17461 aatcagataa cttactttct taaccatttt cctgccttct ttataggttt ttgctcaggt
    17521 cttattttgt tgttcgggat ggtatggcta cgatggcgat ctcaaagccg cttgaaactt
    17581 tatagtcggc taagccgtta tcctttttta ggtaaactgt taaaacaata cttaactagt
    17641 tattatgcta gagaatgggg tacgttaata ggtcaaggtc ttgatttgat gactatttta
    17701 gacattatgg ctattgagaa atcctcacta atgaaagaat tagctgagga catcagaatg
    17761 agtctgcttg aagggcaagc ctttcacatt aaagtagcta cctacccttt ttttaaaaaa
    17821 gaattaagtt taatgattga atacggtgaa atcaaatcga aactgggggg ctgagttgga
    17881 aatctatgcc caggaaagct gggaacaatt ttttagtcag ctttatcaag tgacacaact
    17941 tattcaacca gctatttttt tagtagttgc tgtgacaatc gtcatgattt atgcggcaat
    18001 cctattacca atttaccaaa atatgggagg tattttttaa gatgattaat caatggaaca
    18061 acttacgaca caagaagcta aaaggattta ctcttctaga aatgttattg gtgattcttg
    18121 tcatcagtgt tttgatgcta ttatttgtgc ctaatttaag caagcaaaaa gacagggtta
    18181 cagaaacagg taatgccgct gttgttaaat tagtggagaa tcaagcagaa ctatatgaat
    18241 tatctcaagg ctcaaaacca agtttgagcc agttaaaggc agatggtagt atcactgaga
    18301 aacaagaaaa agcttatcaa gactattatg acaaacataa aaatgaaaaa gcccgtctta
    18361 gcaattaaag cttttacttt gcttgaaact ttactaagtt tgtcagtgat gagtttcatt
    18421 atattgggat tgtctgtacc agtgacaaaa agctatcaaa aagtagaaga gcatctgttt
    18481 tttagccatt ttgaacattt gtatcgacat cagcaaaagt tagccatatt acagcaaaaa
    18541 cagcgcgtct tggatatttc ttcaacaaaa attgttacag aaggtaatag cctaacagtt
    18601 cctaaatcaa taacagtgaa tcatccttat cggttagtta ttgatcagat gggaggcaac
    18661 cattccttag ctaaaattat ttttgacatg actgatcgac gctttaaata ccaattttat
    18721 ctagggagtg ggaattatca aaaaacaagt caaagcttac atagcccttg aaagtatgat
    18781 cgcaacaggc atcctcttta gtattgtcat tctcgtcttg agcagtttac agcagagtca
    18841 ggctgctcta acgtactatc gaaagcagca agaaaagctt aatctagctt tgatggcagt
    18901 acaaactaga actaaagaga tgacattgaa tggttgtcat attactattt tacgtacgga
    18961 tagatatatt agtattcacg atgacgaagg agaagtgatg aagattgagt aaacaattaa
    19021 gtaacataaa agcttttacc cttctagagg cgttaatagc cttactcgtg atatcagggt
    19081 ctttattggt ttatcaaggt ttgacccaga ccctccttaa acgtagccat tatctagccc
    19141 gtcatgatca agacaattgg ctcttatttt ctcatcaatt gcgagaggag ttaagtggag
    19201 caagatttta caaagtagct gataataaac tatacgttga aaagggaaag aaagtactag
    19261 cttttggcca atttaaaagt catgatttcc gaaaatcagc tagtaatgga aaagggtatc
    19321 aacccatgtt atttggaata tcacgtagtc atattcacat agggcagtca cagatttgca
    19381 ttactttaaa gtggaaaagt gggttagaaa ggacttttta ttatgccttt caagactaat
    19441 ctcaaagcag gcatcctttt gtatgctatg tttatggcta gtattttttt gctggtttta
    19501 caggtttacc tttctcaagt gacagctcta cacaaggaat accaagctca aactgactat
    19561 gttaaagccc gactgatagc agaaatagtt tatcaagatc atagatataa agcttctaat
    19621 cctgtctttt ttaaaggtgg tcaagtcatt tgtcgagagc gaaaagagag atggatgtta
    19681 attgttaagt tagatcagca acgacagtat cagtttgaat accttaaacc caaacaatat
    19741 agtcaataaa tagtattgtt gtcgtaaatc cttaaaatga tccaatatat agtaattaaa
    19801 ggtgacactt cttttcattt caagtgtaat ttggtatgat aaggttatga cttttgaaaa
    19861 aattgaagaa gcttatcagc tgctcttaga gaactgtcaa ctaattgaaa atgatctaaa
    19921 aacacatata tacgatgcca ttgttgaaca aaactccttt tatttagggg ctgagggagc
    19981 tagtcctcaa gttgctcaaa atagtgataa actgaaagcc ttgtgcctga caaaagaaga
    20041 atggcgtaag gcctaccagt ttctttttat taaggcagct cagactgagc aactccaagc
    20101 caaccatcag ttcacaccag atgctattgg cttcattctg ctgtatcttt tggaacaatt
    20161 gagtgataaa gatagcttag aggtacttga gattggaagt ggaacaggga acctagccca
    20221 aacccttctc aacaacacga gcaagagcct tgattatgta gggattgaac ttgatgatct
    20281 cttgattgat ctgtcagcca gtattgctga gataatggat tcttcagctc attttattca
    20341 agaagatgcg gtaaggcctc aattactaaa agaaagtgac attgtcatca gtgacttacc
    20401 agttggttat tatcctaacg atgatattgc caaacggtac aaggtggcta gttcagataa
    20461 gcatacctat gcccatcatt tattaatgga acagtcttta aaatacttga aaaaagacgg
    20521 ttttgcgatt tttctggcac cagtcaattt attgacgagc cctcagagcc agttattgaa
    20581 acagtggtta aaagattatg ctcaggtggt ggccttgatt acgctaccag attctatttt
    20641 tggtcatccc tcaaatgcca agtccattat tgtcttacaa aaacaaacag accacccaat
    20701 ggaaaccttt gtctatccaa ttcgggattt gaagcttgca gagaatattc atgattttat
    20761 ggaaaatttc aaaaaatgga aactgagtaa tgtcaattaa ttgatttttt gttataataa
    20821 aaaagcatca cagaaaacga tttcaagaga ggttaaaaat gtcaaagaca attgcaatta
    20881 atgcaggtag ttctagttta aaatggcaac tttatcagat gccagaagaa gaagtgttag
    20941 cacaaggaat tattgagcgt atcggtctca aggattctat ttcaacagtt aagtacgatg
    21001 gcaaaaaaga agagcaaatt cttgatattc acgatcatac agaagctgtt aaaatcttat
    21061 tgaacgattt gattcacttt ggaatcattg cagcttatga tgaaattaca ggagttggac
    21121 accgtgttgt tgctggtggt gaacttttca aagaatccgt tgtggttaat gataaagttc
    21181 ttgaacagat tgaagaatta tccgttttag ctccacttca caatcctgga gcggcggcag
    21241 ggattcgtgc cttccgtgat attttgccag atattaccag tgtttgtgtg tttgacacgt
    21301 catttcacac tagcatggca aagcatactt acctttaccc aattcctcaa aaatattata
    21361 cggactacaa ggttcgtaaa tatggggcgc atgggacaag tcataaatat gtagcgcaag
    21421 aagctgctaa aatgcttggt cgtccattag aagaattaaa actaattact gctcatattg
    21481 gaaatggtgt ctcaattaca gctaattacc acggtaaatc agttgatacc tcaatgggat
    21541 tcactccatt agctggtcct atgatgggaa cacgttctgg tgacattgac ccagctatca
    21601 ttccttattt aattgagcaa gacccagaat taaaagacgc tgctgacgtt gtgaatatgt
    21661 taaacaaaaa atcaggtctt agtggggtgt caggcatttc tagtgatatg cgtgatatcg
    21721 aggctggttt acaagaagat aatccagatg ctgttttagc ttacaatatc tttattgatc
    21781 gcattaaaaa atgcattggt caatatttcg ctgttttgaa tggagcagat gctttggtct
    21841 ttacagctgg tatgggtgaa aatgcaccat tgatgcgtca agatgttatt ggtggcttaa
    21901 catggttcgg aatggacatt gatcctgaga aaaatgtttt tggataccgt ggtgaaattt
    21961 caactcctga gtcaaaagtg aaggttcttg ttatctcaac agacgaagaa ttgtgcatcg
    22021 cgcgtgacgt tgagcgtttg aaaaatacaa aataaaagag actaagtctc aatagattaa
    22081 ccaagtaagg caacacttta cttggttttt tatgaggttt atctgttagt tatggtgcct
    22141 aggtattagt tctgatcagt ctccttgctt tttgtaatct tgtgctaggc gatttggatg
    22201 tcagccttat ttagaagttt ttaattgctt caacgctttt cttgtatgtg tttatagagc
    22261 ccaatgatct aaaagactat ttgataacgg tttatgatga gcgtaatcgt gcgattcgaa
    22321 aacagacaca agtttactgc ttttacctga tgattgttct atttgttgcc ttgttctttt
    22381 tcttcccttg cttaaccatt cgagaccttg tttcttataa gctagctttg atttttgctt
    22441 acctttactt gattatcaga agtctggtgg ctaagaaaat ggaggcacta gggaaaataa
    22501 caactaatag tcaaaagcct ccgtttgata cgagtcaggt tctttgctag atgacttgtc
    22561 taacggttga gtttcctgtc tggtcaacgg aggtgtttgt attgttgtct gatttgtatt
    22621 ggggcactta ttttacaagc ttttagcctt atcgatggtt ttgtcaatgg cagagctgac
    22681 agtagttgtg aggccaaggc gttctaactc catcagacca gcaatagttg tgccaccggg
    22741 gctacaaata gcgtcaatga aatcgtgcgg actttgagaa ctggtcttga gattgctggc
    22801 actagccaag actgtttgag taacaatctc cagcgccttt gctttaggca tgccattctt
    22861 gacgccagcc ttagccaaag cctcaataaa gagataaata taggcaggac ttgaccctgc
    22921 taaagcggta aaggtgtcaa aatccttttc actaatatca aatgtgctac caaagctatc
    22981 tgttaagtct cgaacacgtg cttgtaattc ctgggacacc aaagcatttc ccgttaaagc
    23041 ggtactgctt tggagaattt gtgcattcat gtttggcatg atacgcagca gcggaaggtc
    23101 ttgtcctacg aatgttgcta gtcgttgaag ggaaatgcct gctgccatag atataatagg
    23161 ctgtttgaag tgaagcggtt tgagtaccgt ttcaaatagt tgaggcttga tgcctaaaat
    23221 aacaagatca acctggtcaa taaggtcttg gtgggacata gcataaggca gtgctaactg
    23281 ctccgcaatt tccttggacc gttctaaaga tgatcctgaa atgataagtt catggggtgt
    23341 ttgtttaagg cctttgatga tagcgctagc cattttgcca ataccaataa tgccaatttt
    23401 cattctgttg ctccttgtgg gatattatgg ttaatcaggc taatgctagt ttagtaacat
    23461 ttaatcaagt caacggttga gcgatccagt tttttgataa tggcttgtaa gaaggcttgg
    23521 gcttctacga aatcatccat agcgtagagg gtttgatgag agtgaatgta gcgtgcacag
    23581 actccgatgg ttgttgatgg gacaccacca ttttgaaggt gtgcagcacc tgcatctgtg
    23641 cctcccttgc cacaatagta ttggaaattg acaccagctt cctcagcagt agtcagtaag
    23701 aagtcgcgca tatctttgag catgacatgg cctgggtcgt agaaacgcaa caaggtgcca
    23761 tctccgattg ttccaggatt gccataaata tcaccagcag gcgaacaatc tactgcgaaa
    23821 aagagttcag ggtcgaactt ggtggttgag acgtgggctc cgcgcagacc aacttcttct
    23881 tgaacgttag cacctgcaat tagggtgttg ttaaggtctt gtccttttaa cgcttcaagc
    23941 atttctgtta tcatgagaac accatagcga ttgtcccaag cttttgaaat aatatttttt
    24001 tgattggctg ttaggatcgt ttcagattga gggacaataa tatcacccgg tgtaatacca
    24061 aatctttcag cttctgcctt atccgtaaag ccaccatcaa acacaatatc ttcgatatgt
    24121 ggtagactag cagagccatt tgccccacgg agaaaatgag gaggtaccga tcctgaaata
    24181 aggggaataa cttggccagt gcgtgtgtaa agggtaaacc gttgtgaact gacaacaagt
    24241 gggttccaac caccgatacc aaccacgcgt agcgttccgt caactttgat atcactgacc
    24301 ataaaaccga cttcgtccat gtgcgcagct actaaaatac ggggggcttt ttcagcttta
    24361 ctatctctga taccaaaaat gccaccaagc ccgtctgttt caacacggtc aaccagcggg
    24421 gttattttgg tgcgtaggta gtcacgaacg ctatgttcat agcccgcaat gccatccagt
    24481 tcggtaactt ctttgatttt tgagaataag tctgtcattt ttcatctcca tttacaaggt
    24541 ttcagaccag ttcaacgcgg tcatctccct atattctacc actttttaag taagaatggg
    24601 tttcacagca aagaaaaatc ccagagacta gagatctctg gaagaagtgt ttgtcttttg
    24661 gcgataacct gtttttttag gcagcaagga aatcattaaa atgataaagc acaagctggt
    24721 taataagaat agtagggcgc ttaggatgcg aaaagactct tcactagcga aaagtaagga
    24781 taatggagtc ataaggagaa ccttccattt taggttaatc agtgattttt atagtgtggt
    24841 taaattaata ataaacaaca cctaatcaat gtatttttag tttagttgtt tttactattt
    24901 ttgtcaatac ataacagtag aaatctttag ttttcttatt tcagatcgtc caatttgatc
    24961 tttttatgat aaaatgaggg atatgagtaa aaagaaaata ggtatgattt caggtatctt
    25021 tggatttagt ttagctattg gcctaggaat agttatcaaa gattattgcc aagacaggca
    25081 gcgtcgacaa atgacaaggg atttacgcac ctttttttca cctttaggac aaatcgaagt
    25141 tttatatatc aatccttgtc aggtcaaaca agattatatt tcaggtgggg ttgttatgtc
    25201 aaacggcaaa cagtaccaat tcacctatca cagtcgacaa attagttttg aggaggacaa
    25261 gggatgatca gaccgacttc ttatgagtca ttagcggctc ttattgaaaa agaagataag
    25321 ctggtgttat tttttacggc agactggtgt ccagactgtc aatttattta tcccattatg
    25381 ccagaaattg aagcagaact tacagatatg acttttgttt gtgttaatcg tgatcagttt
    25441 atagaggtag cacaaaagtg ggatattttt ggtattccta gttttgtcgt gattgaaaaa
    25501 ggtcaggaag tagggcgtct ggttaacaaa atgagaaaga caaaaactga gattatgcat
    25561 tttttagctg cttatcaata agaaaagagg aagaataaat gatttttgca tacaataaag
    25621 aacaagttgg cgatgtcttg atggttatct tacaagacac caaagatatc aaacgtcaag
    25681 tagaacgaaa aggcaaagta gcccgtgttt ttgcagaaga aagcggcaaa acccttgctt
    25741 ggaatatctt tgaagcgtca agcttgatta ctattgaagg caatggacag atttttttga
    25801 cagacgagga ccttgcaaga ttaaatgcag agcttgctaa ggaaggattt tcagaaaggc
    25861 ttgaaccgac tgtgggacct gtttttgtgg ttggtcaaat tgttgagatg gtggctcatc
    25921 cagatagcga ccatcttaat atctgtcaag tggctattgg tgaagatcaa acggttcaaa
    25981 tcgtagcggg cgcaccaaat gctgcgcttg gtttaaaaac gattgttgcc ttaccaggtg
    26041 ctataatgcc aaatggtagt ctgattttcc caggaaaatt gcgtggtgaa gaaagttatg
    26101 gtatgatgtg ctctcctcgt gagttagcct tgccaaatgc accgcaaaaa cgtggcatta
    26161 ttgaacttga tgagtcagct gtggtaggag aagcttttga cctagccaaa cattggaaag
    26221 gttagaaaaa ccagctgaaa taagactgta tttaactaaa tgaccagaaa aaaaggatta
    26281 ggagccccta gtcctttttt cttatgtttt caaaaacgtt taaaatgaaa cttattttac
    26341 cacaattttt acgaataata agataaggag gtcagtatgt ataataaagt gatagcaatc
    26401 ggtcgtttgg tggctaaacc agaattggta aaaaccgcta cggataagca tgtagcacgt
    26461 ctctctttag ctgttaatcg aagatttaaa aatgcttctg gagagcgaga agcggatttt
    26521 atctctgttg ttgtttgggg aaagttagca gaaactctgg tttcttatgc tagcaaaggt
    26581 agtttgatgt ctattgatgg cgaacttagg acccgcaagt atgataaaga tgggcaagtg
    26641 cattatgtga cagaagttct ctgccaatca tttcaactgc ttgaaagtcg tgctcaacgc
    26701 gctatgagag aaaataatgt tactaatgat ctagttgatt tagtcttaga agaagatact
    26761 cttccctttt gataggggat aatgcttctc atcacggttc agctgattac tgaaatatat
    26821 gatgaggtga tatccgagca acaaaagtgt gaccagatac ttctgataaa aataaaaaaa
    26881 caccttacga gtatgaacaa agtttcaagt gctagatgtt ggaactttgt tcatcatagg
    26941 aatagtaggg tgttttttag tgtattactt ctttttgttg ctagtgttaa ggtttacttg
    27001 cttacaaacg gttatcaggc cactagaact cttataataa atggagctct ttgagtttgg
    27061 tgtcaatcat ttttaagaca gtcgccaaat cttgtgggtt ttgaacgaaa tcaagggaat
    27121 tgccatcaat tttcattttt ggagagacct tgtagtcttc gtaccaggct gggtattcac
    27181 cgtggacttg atggtaatat tgctcaagac taggattgcc gtcgacctgc tcaaaacttc
    27241 ggccacgttt ttcaatgcgc tcaagcattt tgtcaaaaga cacatcaatg tagatgagaa
    27301 gatcagggcg ttttttaggc atgccttcta gttcttctaa catgttggcg agaagttcct
    27361 gatagatgtc aagttctgtc ttagtgacat tgccattttt gtagttaagt tttaaaaaga
    27421 gttcgtcttc aaaaatagag cggtcaagaa tgttgttatc ggcttgataa gcttctttga
    27481 tggacttaaa ccgtttattc aaaaaataaa tttgaagtaa aaaggcgtac tttttagggt
    27541 cttggtagta gaggtctagc acggggttat tatcaacagc ttcgtaaaaa acgtcagttc
    27601 ctaaatgctc tccaagtgca gcagcaagcg agctcttgcc agccccaatt gttccagcta
    27661 gtacaatcaa catgcgtctc cttctaatgt cattagcgta ctgtttcgaa gatggcttca
    27721 acctcagcta gagtctcagc gcgtgaaaca gcaccacgaa ctttggcagc acctgctgtt
    27781 ccgcgaagat agtgtggcgc taagccccga aattcccgaa cagcaatggt ttcacctttg
    27841 aggttaatca ggcgtttgag gtggtctttg gcgatgtcta attttttggc aaaaggaaga
    27901 tcagccaatt cttgaccagt ttcaaagaaa tgattgattt gggtaaaaag ataaggattg
    27961 ttcatggctg cgcggccaat catcacggca tctacaccga tttcttcaat cataaatttg
    28021 gcgtcttgca ccgaacgcac gtccccattt ccaataaagg gaatcttagt gatagcctta
    28081 gaaactcggg ctaatgtctc atggtcgcag gttcctgtat acatttgctc acgggtacga
    28141 ccgtgcatgg caagagcaga tacacctgcc gactcagccg caagggcatt ttcaacagca
    28201 agtgaactat ccgcccaacc tgtacgcatt ttgacagtca gtggaatatc taaaacagac
    28261 gtgacttcct tgacaatgtg gtaaatctta tcagggtcac ggagccactt ggcaccagcc
    28321 tcgtttttga caactttatt aacagggcag cccatattaa tatcaacaat atcggccttg
    28381 gtattggttt gaatgaaatc agctgcacgt ttcaaccctt ctgcgtcacc accaaataat
    28441 tgaatggaca tagggtgctc attttcatca atatggagca tgtgaagggt cttttcgttg
    28501 ttgtagagca gccctttttc ggaaatcatt tccataacca ccaaaccagc accaaattct
    28561 ttagcgatgg tacgaaaggc agagttggtc acgccagcca tgggagcaag gacagtgcgg
    28621 tgagggattt ccacatcacc aatcctaaag gaagaattaa gctttatcat tgatgagggc
    28681 ctccaaatca ctttcattaa attggtactt ggtaccacaa aattggcaaa caatctcagc
    28741 acccttatct tcgtcaatca tggcctgtaa atctgctttt ggcaaagtca ttagggctgc
    28801 ttcaaaacgt tctctagaac agtcacactg aaaactcaga ggttcttcag aaagacgttt
    28861 gtaaggttca tctccataga tggcctccaa aagggaatcc acatgatttt tggaagctag
    28921 taggtgagaa atggcaggca tttcttgcag gcgtttttca tagcgagcaa tctcttcttc
    28981 agaagctcct ggtagcacct gcaccataaa gccaccagca accttaacct tatcattttc
    29041 atctaaaagc acattaagtc cgattgcaga tggtgtttgc tctgattcgg ttaagtagta
    29101 ggcaaaatcc tcaccaattt ctccagtaat aagaggcgtt gttgaggtgt aagggttacc
    29161 tgtgccataa tcaataatcg tcacaaaatg accattcccc ataaaaggcc caacaaggac
    29221 ttcacctgtt gcggtctttt taatatcgac accagtattt tgaatgtaac ctttcacatg
    29281 tcctttggta tctgccacag agataatgtg tccaaaagaa gaatcaccaa tcactttgac
    29341 ggtgatttta ctatcgcctt tttgattagc cgccaaaatc tggttggcga tcaaggtacg
    29401 gccaagcgca acagtagacg atgacagcgt gttgtgtttt tcctgagcta atgcaaccgt
    29461 ctcagtgcta tcaaggacat aagccctaaa agcccctgat tgtgctattg atttaattat
    29521 tttatccata acttagatta tagcataaag tgtaaaactt gtattgacag gattgcttga
    29581 aaaatcaata cagatagaca gtaaaaaatc ccagagaatg actcgggatt aagttaaggt
    29641 gtggtctaaa gtcgcgcaat gtggtgacat ggcgcttatg ttaattgttt ggttaaatca
    29701 gcttggaatt tttcctcttt aacttgatac atcaattctt ggatagacag aatcgattca
    29761 tcaaaagata tttcagcaac agcaattcct tttgtaagct catgctgaac aaaaggaatc
    29821 agttggctgt gagtgatgac taaatctggt tttaaatcag gaatttgata aacattatct
    29881 tgcaatagat aataggaatg aaaatcaatg cttttatccg agaaatacct tggaaaagaa
    29941 tccgttagga gatgagcatt gataaaatta ctggctacga aaacaacaac taaaggaggt
    30001 tggatatttc ttagaatttg ctcgacatag tggcaaaaaa gatgaaaatg cttatggttc
    30061 aagtcacgct taccaggaag tttggccatc cattcttcga caattaactt taaggacgta
    30121 tagagttttt ggtttccttt atagtacgga gaaacgaata agttggtctc aggaataaaa
    30181 tgttgcagat taaacaagaa tgattttgaa aaaaacataa gagcttttac taaactagcc
    30241 ttttgctctt ttaggttagg taaaagagtg atgataggat ttaaaagcag gcgaaaagta
    30301 tcattttctt caaaaagttg acaacattgt ctgatatgct caggtgtcca ttgtaagctc
    30361 gcaaaagaat tattagcggt gatataaatt aaatagaggt agtccaaatc tcccgctgaa
    30421 aagtttagtt ggcagtaagt ttcgataata tcatggctac tttttttcaa agaatcgtag
    30481 acaaaaagtt ttttt
//
LOCUS       DAUCLR010000012        29517 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.012, whole genome shotgun sequence.
ACCESSION   DAUCLR010000012 DAUCLR010000000
VERSION     DAUCLR010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29517
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.012"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(21..1553)
                     /gene="ahpF"
                     /locus_tag="VGT87_000436"
     CDS             complement(21..1553)
                     /gene="ahpF"
                     /locus_tag="VGT87_000436"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEQ3813322.1"
                     /translation="MALSPDIKEQLAQYLTLLEADLVLQVSLGDNEQSQKVKDFVEEI
                     AAMSERISIENITLDRQPSFKVAKKGHDSGVVFAGLPLGHELTSFILALLQVSGRAPK
                     VDQDVIDRIKAIDRPLHFETYVSLTCHNCPDVVQALNIMSVLNDKISHTMVEGGMFQD
                     EVKAKGIMSVPTVFLDGEEFTSGRATIEQLLEQIAGPLSEEAFADKGLYDVLVIGGGP
                     AGNSAAIYAARKGLKTGLLAETFGGQVMETVGIENMIGTLYTEGPKLMAEVEAHTKSY
                     DVDIIKAQLATSIEKKENIEVTLANGAVLQAKTAILALGAKWRNINVPGEDEFRNKGV
                     TYCPHCDGPLFEGKDVAVIGGGNSGLEAALDLAGLAKHVYVLEFLPELKADKVLQDRA
                     AKTNNMTIIKNVATKDIVGEDHVTGLSYTERDSGEDKHLDLEGVFVQIGLVPNTAWLK
                     DSGVNLTDRGEIIVDKHGSTNIPGIFAAGDCTDSAYKQIIISMGSGATAAIGAFDYLI
                     RQ"
     gene            complement(1574..2134)
                     /gene="ahpC"
                     /locus_tag="VGT87_000437"
     CDS             complement(1574..2134)
                     /gene="ahpC"
                     /locus_tag="VGT87_000437"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002273221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEQ3813323.1"
                     /translation="MSLIGKEIAEFSAQAYHDGKFITVTNEDVKGKWAVFCFYPADFS
                     FVCPTELGDLQEQYKTLKSLGVEVYSVSTDTHFVHKAWHDDSDVVGTITYPMIGDPSH
                     LISQAFEVLGEDGLAQRGTFIVDPDGIIQMMEINADGIGRDASTLIDKIHAAQYVRKH
                     PGEVCPAKWKEGAETLTPSLDLVGKI"
     gene            2540..2610
                     /locus_tag="VGT87_000438"
     tRNA            2540..2610
                     /locus_tag="VGT87_000438"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2572..2574,aa:Cys,seq:gca)
     gene            3119..3322
                     /locus_tag="VGT87_000439"
     CDS             3119..3322
                     /locus_tag="VGT87_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEQ3813324.1"
                     /translation="MAQGTVKWFNAEKGFGFISTENGQDVFAHFSAIQTNGFKTLEEG
                     QKVAFDVEEGQRGPQAVNITKLA"
     gene            3518..3979
                     /locus_tag="VGT87_000440"
     CDS             3518..3979
                     /locus_tag="VGT87_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEQ3813325.1"
                     /translation="MPTKNTSDSIEEYIKELLAKSGIAEIKRSMLADSFQVVPSQINY
                     VIKTRFTESRGYEVESKRGGGGYIRIAKVHFSDKHHLIGNLMATIEDCISEQVFTDSI
                     QLLFDEHLLTEREGNIILAVASDDVLGTDGSTIRARMLYRLLQRIDRKGSN"
     gene            3979..6423
                     /locus_tag="VGT87_000441"
     CDS             3979..6423
                     /locus_tag="VGT87_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEQ3813326.1"
                     /translation="MIMYSTKMQDIFRQAQFQAARFDSHCLETWHVLLAMVAVDNSLA
                     NMILSEYDAQVAIEEYEAAAILAMGKPPKEQLSRVDFRPQSKTLTNLLAFAQAISQIT
                     RDQEVGSEHVLFAILLNPDIMASRLLEIAGYQIKDNGNGQPRLADLRKAIERHAGYSK
                     EMIKAIHELRKPKKTKTQGTFSDMMKPPSTAGELSDFTRDLTEMARQGLLESVIGRDQ
                     EVSRMIQVLSRKTKNNPVLVGDAGVGKTALAYGLAQRIANGAIPYELKEMRVLELDMM
                     SVVAGTRFRGDFEERMNQIIDDIEADGQIILFVDELHTIMGSGSGIDSTLDAANILKP
                     ALSRGTLHMVGATTQEEYQKHIEKDAALSRRFAKILIEEPNTEDAYQILMGLKLSYET
                     YHNVSISNEAVKTAVKMAHRYLTSKNLPDSAIDLLDEASAAVQNMVKKSAPETLTPID
                     QALINGDMKKVSRLLAKEAKGQMRKPTPVTEDDILATLSKLSGIPLEKLTQADSKKYL
                     NLEKELHKRVIGQDAAVTAISRAIRRNQSGIRTGKRPIGSFMFLGPTGVGKTELAKAL
                     AEVLFDDEAALIRFDMSEYMEKFAASRLNGAPPGYVGYDEGGELTQKVRNKPYSVLLF
                     DEVEKAHPDIFNVLLQVLDDGILTDSRGRKVDFSNTIIIMTSNLGATALRDDKTVGFG
                     VKGIHQDHQAMEKRILEELRKTYRPEFINRIDEKVVFHSLTQDNMRDVVKIMVQPLIT
                     TLAEKGITLKIQPLALKHLSEVGYDEHMGARPLRRTLQTEIEDKLSELILSRELTSGH
                     TLKIGLSHGKLTFHIA"
     gene            6601..6891
                     /gene="groES"
                     /locus_tag="VGT87_000442"
     CDS             6601..6891
                     /gene="groES"
                     /locus_tag="VGT87_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEQ3813327.1"
                     /translation="MLKPLGDRVVVRFDDEKEQTVGGFVLAGTHKESTRKATVLAVSE
                     TGVRTITGDSVLPSVSVGQEVLVENGHDLEVTVDDEKVSIIRESDIIAIVTK"
     gene            6927..8558
                     /gene="groL"
                     /locus_tag="VGT87_000443"
     CDS             6927..8558
                     /gene="groL"
                     /locus_tag="VGT87_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880885.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEQ3813328.1"
                     /translation="MAKDIKFSADARAAMVRGVDMLADTVKVTLGPKGRNVVLEKAFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVHEG
                     LKNVTAGANPIGIRRGIETATATAVEALKAIAQPVSGKEAIAQVAAVSSRSEKVGEYI
                     SEAMERVGNDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDNEKMVADLENPFI
                     LITDKKVSNIQDILPLLEEVLKTNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATMTALGQAAKITVDKDSTVIVEGS
                     GSSEAIANRIALIKSQLETTTSDFDREKLQERLAKLAGGVAVIKVGAPTETALKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALITVIEKVAALELEGDDATGRNIVLRALEEPVRQ
                     IALNAGYEGSVVIDKLKNSPAGTGFNAATGEWVDMIKTGIIDPVKVTRSALQNAASVA
                     SLILTTEAVVANKPEPAAPAPAMPAGMDPGMMGGF"
     gene            8810..9055
                     /locus_tag="VGT87_000444"
     CDS             8810..9055
                     /locus_tag="VGT87_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ3813329.1"
                     /translation="MNHFGEIFKTFRESRGLRLKDVAKAGISTSQLSRFEKGETDLTI
                     STFMLILDESNMSIDEFMYAVHDFHRDDTTQDSDGLK"
     gene            complement(9760..11259)
                     /locus_tag="VGT87_000445"
     CDS             complement(9760..11259)
                     /locus_tag="VGT87_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HEQ3813330.1"
                     /translation="MINKKISLGVLSILTAFSLQSVSYACTGFIIGKDLTKDGSLLYG
                     RTEDLEPHHNKNFIVRLAKDNPAGEKWKDPSNGFEYPLPEHSYRYSAIPDVTPNKGVY
                     DEAGFNEFGISMSATVSASANDAIQKIDPYVKNGLAESSMASVILPSVKTAREGVALI
                     AKIVTEKGAAEGNIVTLADKDGIWYMEILSGHQYVAIKFPDDKYAVFPNTFYLGHVDF
                     NDKENTIASEDVEKVAKKAKSYTEVDGKFHIAKSYNPPLNDANRSRSFSGIKSLDPDS
                     KVTYKDSNYELLQSTDKTFSLEDAMKLQRNRFEGLDLKPLDQMALDGKGKPKSKKAVK
                     GYAYPISNPNVMEAHIFQLKKDIPAELGGGVMWLSIGSPRNAPYLPYLGNISRTYEAY
                     QEKSTQYNDKSWYWTVSHINDLVAAHPKPFGTKVIDEMKGLEKTWIAEQDKSTKEISD
                     LVVSDPKAAQEKADKISLDRAEKTFKRLKAIEAKLVEEKPKNKKGLNRS"
     gene            11506..13146
                     /locus_tag="VGT87_000446"
     CDS             11506..13146
                     /locus_tag="VGT87_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813331.1"
                     /translation="MTKPFHHKKLKQITIIAATSLFLFLIGGAFYYSKNHCINAYLKA
                     RSAQSGPVFENIKAYLVWDDTNEQITNDEAMYTKFRRYSQKELRQKKQDLKAASQDSA
                     VQVKSVGRRFWIFPDYRIAIKPMDLTIKTNVPQADVLLNHKKVAVSDSEQFSVKLDRL
                     PTAEYTASIRGKHNGRNIKVNKSYDGDNPVLDLSVSFRTFLVTSNAKQGDLYFDDNHI
                     GTLKDGQLQVEDYPVTENAQAYMKTTFPDGELRSQKYALADVEEGATLEILVTDLLEE
                     DKAGELLVSAFDQLMHYLSTGQDSSNLRSVFEAGSSNAFYRGLKESIKAKFQTDTRKA
                     SRLNIPSILLTTMTQVGKTTYVLDFTATYEFLYDNSTDPEQHTSGHINQDLIGKVTVK
                     KVGQHYLISQSGSKNITVVKEDNQLKAPSVFPESILGTWTGQANGLSIHMSLASDGTI
                     TTKVEDQKGNRSKETRTAKISKVEDKGNGFYLYMPDPGSDISALVPEGGLGGANVKYA
                     YGFKISGKTASPVVWQAALTHEFDYTKPLSGVTLQKQP"
     gene            complement(13235..14236)
                     /locus_tag="VGT87_000447"
     CDS             complement(13235..14236)
                     /locus_tag="VGT87_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEQ3813332.1"
                     /translation="MTSVKVLKLSLLLLPPKWLSTLLKNTITGSMKTTNKPSKGFTVT
                     LKNPFEELTVKALLEEQLLIPRKIRHFLRTKKHVLINGHPVNWQSCVKYGDQVKLFFD
                     HEDYPEKIIVMGQAEKVTCLYEDEHIIIVNKPEGMKTHGNDPTELALLNHVSAYTGQT
                     CYVVHRLDKETSGAILFAKTPFILPILNRLLEKRDIHREYLALVHGSLDSPRVTYHHP
                     IGRHRHDRRKRVVDPINGKKAITEVTLVKNFHKTASLLNCQLQTGRTHQIRVHLAHQG
                     HVLFGDPLYSNGKKDCARLMLHAYQLRLKNPLTQEDICVQAKSATFDAVLNAQKARK"
     gene            14812..15192
                     /locus_tag="VGT87_000448"
     CDS             14812..15192
                     /locus_tag="VGT87_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEQ3813333.1"
                     /translation="MKTIRRYDVNEDRGHTGLVEAGDFYYLNYCVGNVGQDIESQING
                     AFDEMERRLALVGLTLDAVVQMDCLFRDVWNIPVMEKMIKERFNGRYPARKSIQTEFA
                     HHGGPQGLLFQVDGVAYSKHISMT"
     gene            15231..17567
                     /locus_tag="VGT87_000449"
     CDS             15231..17567
                     /locus_tag="VGT87_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEQ3813334.1"
                     /translation="MRFLELLQKKFFPKAYQEKQFLMHQKTRLTPQHNQKQYSPNANH
                     LDSSATKNSEQDPAIALQRSRAYEGSPKSRPAWLQKLEAVLPSPQHPIRRFWRRYHIG
                     KLLMILIGTLVLLLGSYLFYLSKTAKVSDLQDALKATTVIYDHKGEYAGSLSGQKGSY
                     VELNAISDDLENAVIATEDRTFYSNSGINLKRFLLAVVTAGRFGGGSTITQQLAKNAY
                     LSQDQTIKRKAREFFLALELTKKYSKKDILTMYLNNSYFGNGVWGVEDASQKYFGTTA
                     ANLTLDEAATLAGMLKGPEIYNPYHSLKNATHRRDTVLGAMVDAKKITQTKAQQARAV
                     GLKNRLADTYVGKTDDYKYPSYFDAVISEAIATYGLSEKDIVNNGYKVYTELDQNYQT
                     GMQTTFNNDELFPVSAYDGSSAQAASVALDPKTGGVRGLIGRVNSSENPTFRSFNYAT
                     QAKRSPASTIKPLVVYAPAVASGWSIEKELPNTVQDFDGYQPHNYGNYESEDVPMYQA
                     LANSYNIPAVSTLNDIGIDKAFTYGKTFGLDMSSAKKELGVALGGSVTTNPLEMAQAY
                     AAFANNGVIHPAHLINRIENARGEVLKTFTDKAKRVVSQSVADKMTAMMLGTFSNGTA
                     VNANVYGYTLAGKTGTTETNFNPDLAGDQWVIGYTPDVVISQWVGFNQTDENHYLTDS
                     SAGTASAIFSTQASYILPYTKGSQFHVDNAYAQNGISAVYGVNETGNQSGVDTQSIID
                     GLRKSAQEASQSLSKAVDQSGLRDKAQSIWKEIVDYFR"
     gene            17616..17768
                     /gene="rpmG"
                     /locus_tag="VGT87_000450"
     CDS             17616..17768
                     /gene="rpmG"
                     /locus_tag="VGT87_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEQ3813335.1"
                     /translation="MAQKKASLACVECGSRNYSIGVSSTPKPTRLEVNKFCKYCKTYT
                     LHKETR"
     gene            17782..17958
                     /gene="secE"
                     /locus_tag="VGT87_000451"
     CDS             17782..17958
                     /gene="secE"
                     /locus_tag="VGT87_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEQ3813336.1"
                     /translation="MGFISGTFKVLKDTTWPNRKQRWKDFISVLEYTAFFTVIIYIFD
                     QLLAKSVLALINLF"
     gene            complement(18195..18968)
                     /locus_tag="VGT87_000452"
     CDS             complement(18195..18968)
                     /locus_tag="VGT87_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEQ3813337.1"
                     /translation="MTDRGIVFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCANPESQQ
                     KAPEQMLTSDGLNTKIVGEEKTVDEVIEEVLKDLDFYEESGGGMTLSGGEIFAQFDFA
                     LALLKAAKAAGLHTAIETTAFAKHEQFVTLIDYVDFIYTDLKHYNQLRHQKVTGVRND
                     LIIKNIHYAFQAGKEIVLRIPVIPQFNDSLDDAKAFSELFNQLEIDQVQLLPFHQFGE
                     NKYKLLGREYEMAEVKAYHPEDLADYQAVFLNHNIHCYF"
     gene            19094..19840
                     /locus_tag="VGT87_000453"
     CDS             19094..19840
                     /locus_tag="VGT87_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEQ3813338.1"
                     /translation="MNRLERIIQLVSQKKKIDVNSLSEQLDVSKVTIRKDLDKLESKG
                     LLRREHGYAVLNSGDDLNVRLSYNYNIKRRIAEKAAELVQDNDTIMIESGSTCALLAE
                     VLCQTKRNIKIITNSCFIANYIRQYSSCQIILLGGYYQPNSEVTVGPLLKEMISLFHV
                     NRVFVGTDGFNKDLGFMGKDMMRSEGVRYMADAAEEVVILTDSSKFSKTSLVHQLSLA
                     DVNRVITDQALDKQTQELLSASGLVLDFVS"
     gene            19856..20836
                     /locus_tag="VGT87_000454"
     CDS             19856..20836
                     /locus_tag="VGT87_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEQ3813339.1"
                     /translation="MKEERRRLLAKVAYLHYVQGKSQTLISKEMNIYRTTVCRMLAKA
                     KEEGIVRIEIADYDADLFALEEYVRQQYGLEKLDLVPNQVEDTPMDTLTNVAKTAAEV
                     FRHVVKDGDKIGLSWGATLSCLMDELNPKAMKDVFIYPLAGGPSHINAKYHVNTLVYR
                     LARIFHGNSAFMNAMVIQEDKHLAKGILQSKYFNDILTSWDQLDLALVGIGGEPNSLE
                     QSQWRDLLTSSDHDQLKYEKAVGEVCCRFFDQAGQPVYTGLQDRTIGISLEQLRRVPK
                     TMAVATGKHKAKAILAALKAGFINYLVTDKETMLAVLALDEDIDLNNVLL"
     gene            21124..21444
                     /locus_tag="VGT87_000455"
     CDS             21124..21444
                     /locus_tag="VGT87_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEQ3813340.1"
                     /translation="MQVIVPDQIIMGLILNAGDAKQHIYQALKCAKEDDYATSEKEMA
                     LADDALLEAHNLQTQFLAQEASGNKSEITALFVHSQDHLMTTITEINLIKEIIDLRKE
                     LATK"
     gene            21472..21780
                     /locus_tag="VGT87_000456"
     CDS             21472..21780
                     /locus_tag="VGT87_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEQ3813341.1"
                     /translation="MIKIGLFCAAGFSTGMLVNNMKVAAEKKGIDCQIEAYAQGKLAD
                     YAPLLDVALLGPQVAYTLDKSEAICKDNDIPIAVIPMADYGMLDGNKVLDLALSLVKE
                     "
     gene            21790..23094
                     /locus_tag="VGT87_000457"
     CDS             21790..23094
                     /locus_tag="VGT87_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEQ3813342.1"
                     /translation="MAKMNMQNIIKPIMTFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     AGQIPFQGVNDAIASVFGADWTEPFMQVYHGTFAIMGLISCFAIGYSYAKNSDVEPLP
                     SGVLSLSAFFILLRSSYVPAEGEAIGDAISKVWFGGQGIIGAIVIGLTVGAVYTTFIR
                     RHIVIKMPDQVPQAIAKQFEAMIPAFVIFTLSMLVYIIAKSVTGGGTFIEMIYDVIQV
                     PLQGLTGSLYGALGIAFFISFLWWFGVHGQSVVNGIVTALLLSNLDANKALMAAGELS
                     LDKGAHIVTQQFLDSFLILSGSGITFGLVVAMIFAAKSKQYKALGKVAAFPALFNVNE
                     PVVFGFPIVMNPVMFLPFILVPVLAALTVYGAIAIGFMQPFAGVTLPWSTPAIVSGFM
                     VGGWQGAIVQILILIMSTLVYFPFFKIQDNMAYQNEQASEES"
     gene            23305..25722
                     /locus_tag="VGT87_000458"
     CDS             23305..25722
                     /locus_tag="VGT87_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEQ3813343.1"
                     /translation="MTETKSPYFGHLTDRMTHYREAVLDKKPYIDAERAILATEAYQK
                     HQNKPANLKRAYMLQTILENMTIYIEDESLIAGNQASSNKDAPIFPEYTLEFALNELD
                     LFEKRDGDVFYITEETKQQLRDIAPFWENNNLRARCGVLLPEEVQVYMETGFFGMEGK
                     MNSGDAHLAVNYQKLLEHGLKGFEERARAAKAALDLTIPENIDKYHFYDSVFIVIDAV
                     KTYAKRYAKLARELAKTAKPERQAELLDIARICDKVPYEPAKTFAEAVQSVWFIQCIL
                     QIESNGHSLSYGRFDQYMYPYVKADLEAGRETEDSIVERLTNLWIKTLTINKVRSQAH
                     TFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLRSVAQTKLPQPNLTVRYHKGLDNT
                     FMNECIEVMKLGFGMPAMNNDEIIIPSFIKKGVSEEDAYDYSAIGCVETAVPGKWGYR
                     CTGMSYINFPKILLITMNDGIDPASGKRFAKGYGHFKDMTSYEELKAAWDATLREITR
                     MSVIVENAIDLGLEREVPDILCSALTDDCIGRGKTLKEGGAVYDYISGLQVGIANLSD
                     SLAALKKLVFEEGRLTPEELWQALESDFAGERGEEIRQMLINDAPKYGNDDDYADSLV
                     VEAYDTYIDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGKGTLATPDGRHAGTPL
                     AEGCSPEHSMDKKGPTSVLKSVAKLPTDEIVGGVLLNQKVNPQTLAKEEDKLKLMALL
                     RTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSKATQDDII
                     ERTEHTL"
     gene            25735..26403
                     /locus_tag="VGT87_000459"
     CDS             25735..26403
                     /locus_tag="VGT87_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEQ3813344.1"
                     /translation="MEYMLDTLDLEAIKKWHHILPLAGVTSNPSIAKKEGDIDFFERI
                     REVRAIIGDKASIHVQVIAQDYEGILKDAAEIRRQCGDSVYVKVPVTTEGLAAIKTLK
                     AEGYHITATAIYTTFQGLLAIEAGADYLAPYYNRMENLNIDPEAVIGQLAEAINRENA
                     NSKILAASFKNVAQVNKSFALGAQAITAGPDVFEAGFAMPSIQKAVDDFGKDWEAIHH
                     RKSI"
     gene            26460..27548
                     /locus_tag="VGT87_000460"
     CDS             26460..27548
                     /locus_tag="VGT87_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEQ3813345.1"
                     /translation="MKVFASPSRYIQGKNALFTNAKTLKQLGDSPILLCDDVVYGIVG
                     ERFESYLIDNGMTPVHVAFNGEASDNEISRVVAIAKENGNDVIIGLGGGKTIDSAKAI
                     ADLLAVPVIIAPTIASTDAPTSALSVIYTDEGAFEKYIFYSKNPDLVLVDTQVICQAP
                     KRLLASGIADGLATWVEARAVMQKNGDTMAGGNQTLAGVAIAKACEQTLFADGLKAMA
                     SCDRQVVTPALENVIEANTLLSGLGFESAGLAAAHAIHNGFTALTGAIHHLTHGEKVA
                     YGTLTQLFLENRSREEIDRYIDFYQAIGMPTTLKEMHLDTATQEDFLKIGRQATMTGE
                     TIHQMPFVISPEDVAAALVAVDAYVTSR"
     gene            complement(27630..28139)
                     /locus_tag="VGT87_000461"
     CDS             complement(27630..28139)
                     /locus_tag="VGT87_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEQ3813346.1"
                     /translation="MSTDHHLREVSYQLLAERGGSLEAIAELVLFLQNDYIPNLTMAE
                     CLESVEAVLAKREVQNAIITGVELDKLAEANQLSEPLLSILKTDQGLYGIDEILALSI
                     VNLYGSIGFTNYGYLDKTKPGIVDKLNHKDGYSCHTFLDDIVSAIAAAAASRIAHNDP
                     AKSAITNQQ"
     gene            28503..29318
                     /gene="sdaB"
                     /locus_tag="VGT87_000462"
     CDS             28503..29318
                     /gene="sdaB"
                     /locus_tag="VGT87_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptodornase B"
                     /protein_id="HEQ3813347.1"
                     /translation="MNLLGSRRVFSKKCRLVKFSMVALVSATMAVTTVTLENTALARQ
                     TQVSNDVVLNDGASKYLNEALAWTFNDSPNYYKTLGTSQITPALFPKAGDILYSKLDE
                     LGRTRTARGTLTYANVEGSYGVRQSFGKNQNPAGWTGNPNHVKYKIEWLNGLSYVGDF
                     WNRSHLIADSLGGDALRVNAVTGTRTQNVGGRDQKGGMRYTEQRAQEWLEANRDGYLY
                     YEAAPIYNADELIPRAVVVSMQSSDNTINEKVLVYNTANGYTINYHNGTPTQK"
BASE COUNT         9213 a         5663 c         6164 g         8477 t
ORIGIN      
        1 cttcttttaa atatgctgtt ttattgacga atcaagtagt caaaggcacc gatggcagca
       61 gtagcaccag accccatgga aataatgatt tgtttataag ctgaatcagt acagtcccca
      121 gcagcaaaga taccaggaat attggttgac ccatgtttat ccacaatgat ttcaccgcgg
      181 tcggttaggt tgacaccact atctttgagc caagctgtgt ttggaacaag accaatttga
      241 acaaagaccc cttcaaggtc aaggtgtttg tcttcaccgc tatcacgctc agtgtagcta
      301 agacctgtaa catggtcctc tccaacaata tccttagtag caacattttt gatgatggtc
      361 atattgttag tcttagctgc gcggtcttga aggactttgt cagctttaag ttcaggcaag
      421 aattctaaaa cgtaaacgtg tttagcaaga ccagccaaat caagggccgc ttctaaaccg
      481 gagttaccac caccgataac agcaacatct ttgccttcaa agagaggacc gtcacagtga
      541 gggcagtaag tcaccccttt attacggaat tcatcttcac caggaacatt aatgttacgc
      601 catttggccc caagagctaa gatagcagtt ttggcttgta gaaccgcacc attagctaga
      661 gtaacctcga tgttttcttt tttctcaata gaagtagcta gttgtgcttt gataatatca
      721 acatcataag acttagtatg ggcctccact tcggccatta attttggtcc ttcagtgtaa
      781 agggtaccaa tcatgttttc gatgccgaca gtttccatga cctgaccacc aaaggtttca
      841 gcaagtaaac ctgtttttag tcctttacga gctgcataga tagcagcgct attgcccgca
      901 gggccaccac caataacaag gacatcatag aggcctttat cagcaaaggc ttcctcagaa
      961 agtgggccag cgatttgttc taagagttgc tcaatggtag cacgaccaga agtgaattct
     1021 tccccgtcta aaaagacagt agggacagac ataatgcctt tggctttcac ttcatcttgg
     1081 aacatgccgc cttctaccat agtgtgtgaa atcttgtcgt tcaagacgga cataatgttt
     1141 aaagcttgaa caacatctgg gcagttatgg caggttaagc tgacataagt ttcaaaatgc
     1201 aacggacggt caatggcctt gatgcgatca ataacatctt gatccacttt tggagcacgt
     1261 ccagatactt gcaagagagc aagaataaag gacgtcaatt cgtggcctaa aggaagacca
     1321 gcaaagacaa ccccactatc gtgacctttt ttcgcaacct taaagcttgg ttgtcggtct
     1381 aaagtgatgt tttcaataga aatacgctca gacatagcag ctatttcttc cacaaagtct
     1441 ttgacttttt gagactgctc attgtcacca agggaaactt gcaaaacaag atcagcttct
     1501 aacagggtga ggtattgggc aagttgttct ttaatatcag gacttaatgc catagctttc
     1561 ctcctaaaat ttattaaatt ttaccaacta aatcaagact tggtgtcaaa gtttcagcgc
     1621 cttctttcca tttagctgga caaacttcac ctggatgttt acggacgtat tgggcagcgt
     1681 gaattttatc aatcaaggtg ctagcgtcac gtccaatacc atcagcatta atttccatca
     1741 tttggataat accatctggg tcaacgataa atgttccacg ttgagcaagt ccgtcttcgc
     1801 caagcacttc aaaggcttgt gaaataaggt gtgaagggtc accaatcata gggtatgtga
     1861 tagtgccaac cacatctgaa tcatcatgcc aagctttatg aacaaaatga gtatcagtag
     1921 agacagaata aacttctaca ccaagagatt ttagtgtttt gtattgctct tgaaggtcac
     1981 cgagttcagt tgggcaaaca aatgaaaagt ctgctgggta gaaacaaaaa actgcccatt
     2041 ttcctttaac gtcttcattt gtaacagtga tgaattttcc atcgtgataa gcttgagctg
     2101 aaaattcagc aatttctttt ccaattagag acatatagtt gtcctccttt tttatagttt
     2161 tatacttgta ttataaaaga acaacaagat tatgtctact cagaaaccct tgattttact
     2221 gggaaatcag cgattgtttt ctgacaattc agttgtgaaa taaatcacat taattaaaat
     2281 gattctaaat aagccgaaga cgccgttaaa tcaaaggtta atttaattat aatctacaga
     2341 aaatataatt gttattaaag cgtttacaaa aaactttttt gattccgtta aggcaactta
     2401 atattatggt tacctaatca ctaatattgt aacctatttt ggcatgagat cagggaatgg
     2461 ctgttatatt aattggatat gtcttgtgca aatagtgctt gacggatttg tggaattagg
     2521 gtaaaataat ctttgttgtg gcggtatagc caagtggtaa ggcacggctc tgcaaaagct
     2581 tgatcgtcgg ttcaaatccg tctaccgcct tttatataca aaacaagcct tgattaactt
     2641 cgtgaaaacg ttgttaaatc aaggttttta ttttttgcgg caggataaaa aaagagaaaa
     2701 aaaaaaataa agtcggtcaa atttcccgaa gatgtctttt agagcctgat tattaggtat
     2761 gaaaaatact atggccaaaa ttggtaaacg attgcaaata aaggtcagag ttcttacccc
     2821 cttaaacgat atgcgttaaa agttagttga actgccgtgc accttaacga tagacacggt
     2881 gatgtgagag cgagtagagt tgaatctcta cttgtttacc ttaccatagt cagctttata
     2941 aagtaaccac ccattacaat gcaactatat tcttaatgtt tttaagcatg ttattgacaa
     3001 gtgttataaa atgtgttact cttagtgagt aaattagttt ttgagttagt atttgtgttt
     3061 caacatcttt atttctctct atttttacaa atgattttta gaaaggaagt ggatgtacat
     3121 ggcacaaggt acagtaaaat ggtttaacgc tgaaaaaggt tttggtttta tttcaactga
     3181 aaacggtcaa gatgtctttg cacatttttc agcaatccaa actaatggtt ttaaaacgtt
     3241 agaagaagga caaaaagtgg catttgacgt cgaagaaggt caacgtggac ctcaagcagt
     3301 caatatcact aaattggcct aatctggtta aagtaactaa agagaagcag atgttggaaa
     3361 caacaactgc ttttttgatt tctaccagtt acctttaatg attaagagtt gcgtttaagg
     3421 tcttgtattt gttataataa tagtcagttt tagtcaaaat gtattgacgt ggtgctacta
     3481 agtttttaga gtagcccgca agcaaaggag tgacagcatg ccaaccaaaa atacttcaga
     3541 cagcattgaa gagtatatta aagaattatt agctaagtca ggaatagcag aaattaagcg
     3601 gtccatgcta gcagattcgt ttcaagttgt cccaagtcag atcaactatg tcattaaaac
     3661 ccgttttacg gagagtcgag gctatgaagt agagagtaaa cgaggtggtg gaggctatat
     3721 tcgtattgcc aaggttcatt tttcagacaa acaccatctg attggcaatt taatggcaac
     3781 tattgaagac tgtatcagtg agcaagtttt cacggactcg atacaattgc tttttgacga
     3841 acacttattg acagagcgtg agggaaatat cattttggct gtggcgtctg atgatgtttt
     3901 aggaactgat ggttcaacta ttcgtgcacg aatgctctat cgcttattgc aacgaattga
     3961 cagaaaggga agcaattaat gattatgtat tcaacgaaga tgcaagacat ttttagacag
     4021 gcgcagttcc aagctgctcg ctttgatagc cattgcctgg aaacttggca tgttttgtta
     4081 gctatggtag ctgtagataa ttctttagca aatatgattt taagtgaata tgatgcccaa
     4141 gtcgccatag aagaatatga agctgcagct attttagcca tgggcaaacc ccctaaggaa
     4201 cagttgtctc gtgtagactt cagacctcaa tctaaaacct tgactaactt gttagctttt
     4261 gcgcaggcta ttagccaaat cactagggat caagaagtcg gctctgagca tgtcttattt
     4321 gctattttat tgaatccaga tatcatggcg agtcgtttgt tagaaatagc aggctatcag
     4381 ataaaagata acggcaatgg gcagccgcga ttagctgact tgcgaaaagc aatagaacgt
     4441 catgcaggtt acagtaagga aatgattaag gctattcacg aactacgtaa gcctaaaaaa
     4501 acgaaaacac aagggacctt ttcagatatg atgaagccac caagtacagc tggtgagttg
     4561 agtgatttca caagagattt gactgaaatg gcaagacaag gtttgttaga atcggtgatt
     4621 ggacgtgacc aagaagtatc tcgtatgatt caggtactaa gtcgtaaaac gaaaaacaat
     4681 cctgtcttgg taggtgatgc aggtgttggt aaaactgcgc ttgcttatgg ccttgctcaa
     4741 cggattgcaa atggcgctat tccttatgaa cttaaggaga tgcgtgtcct agaattagac
     4801 atgatgagtg tggtagcagg aacccgtttt cgtggggatt ttgaagagcg catgaatcaa
     4861 atcattgatg atattgaagc tgatggtcag attattcttt ttgttgatga actacatact
     4921 attatgggtt ctggcagtgg tattgacagt acacttgatg cggctaacat tttaaaacca
     4981 gcattatcgc gcggtactct tcatatggtt ggagcaacaa ctcaagaaga atatcaaaaa
     5041 catattgaaa aagatgcagc tctttcgcgt cgttttgcta aaatattaat tgaagaacct
     5101 aatacagaag atgcttatca gattttgatg ggcctaaaat tatcttatga gacctaccat
     5161 aatgtctcga tatcaaatga agcagttaaa acagctgtaa aaatggcaca ccgttattta
     5221 accagtaaaa atctccctga ttcagctatc gatttattag atgaagctag tgctgctgtg
     5281 caaaacatgg tgaaaaaatc agcacctgag actttaacac caatagacca agctcttatc
     5341 aatggtgata tgaaaaaagt atctcgcctc ttagctaaag aagcaaaagg tcagatgaga
     5401 aaaccaacac cagtcacaga agatgatatt ttggcaacct tgagtaagtt atcgggaatt
     5461 ccacttgaaa aactgacgca agctgatagc aaaaaatacc tcaatttaga aaaagaactg
     5521 cataagcgtg tgattggtca ggatgctgct gttacggcta tttcaagagc cattcgtcgc
     5581 aatcagtcag gtattcgaac aggaaaacgt cctattggat catttatgtt tcttggccca
     5641 acaggagtag gtaagacaga actagcaaag gcccttgcag aagttctctt tgatgatgaa
     5701 gcagcgctta ttcgttttga tatgtctgag tacatggaaa aattcgcagc gtctaggctt
     5761 aatggagcac ctcctggtta tgtcggctat gatgaaggag gtgaactgac acagaaagtt
     5821 agaaataaac cttattcagt cttgcttttt gatgaagtgg aaaaagcaca tcctgatatt
     5881 tttaacgttc tccttcaagt attagatgat ggtatattga ctgatagtcg tgggcgtaag
     5941 gtcgattttt caaatactat tattatcatg accagcaatc ttggcgcaac agccctgcgt
     6001 gatgataaaa cggtcggttt tggggtcaaa ggcattcacc aagaccatca agctatggag
     6061 aaacgtatct tagaagaatt aagaaaaact taccgcccag aatttatcaa tcgtattgat
     6121 gaaaaagtgg tctttcatag tctgacccaa gataacatgc gcgatgtggt taaaatcatg
     6181 gtacagcccc tgattactac attggcagaa aaaggtatta cccttaaaat tcagcctttg
     6241 gccttgaaac atttgtccga ggtcggctat gatgagcata tgggggcaag accattacgt
     6301 cgaacgctgc aaactgagat agaagataag ctatcagagc ttatcctttc tcgagaattg
     6361 acaagtgggc atacgctaaa aattggatta tcacatggca aattaacgtt tcacatagct
     6421 taatttccat ataaaaggtg gatcatcctt gatgtcacca aaaaaagagg ttattcttac
     6481 aaaaataact tcttttttct tgactatttt tgaccaagtg ataaaataga atataaagtt
     6541 agcactcgat gaatatgagt gctaacttta ctaaagattc ttttattgga ggaaaatttc
     6601 atgttaaaac cattaggtga ccgtgtggtc gtaagatttg atgatgaaaa agagcagaca
     6661 gtcggtggtt ttgtccttgc aggcactcat aaagagtcaa cacgaaaagc gacagtcctc
     6721 gctgtcagtg aaacgggtgt tcgcactatt acaggcgata gcgtgctacc ttcagtttca
     6781 gtgggtcaag aggttttagt tgaaaatggg catgatttag aagtaaccgt agatgatgag
     6841 aaagtttcta tcatccgtga atctgacatc attgctattg tcacaaaata aaacgacaaa
     6901 tctctaaata gaaagaagga ttgaatatgg caaaagatat taaattttca gcagatgcgc
     6961 gtgctgccat ggtgcgcgga gttgatatgt tagcagatac cgtcaaagta acgcttggtc
     7021 ctaaaggacg caatgttgtt cttgaaaaag cttttggttc tcccttaatt actaatgacg
     7081 gggtaaccat tgctaaagag atcgaattag aagatcattt tgaaaacatg ggagcaaaat
     7141 tggtgtctga agtggcttct aaaaccaatg atattgctgg tgatgggacg actactgcaa
     7201 cagttttgac acaagccatt gttcatgaag gactaaaaaa tgtgacagca ggtgctaatc
     7261 caattggtat ccgtcgaggc attgaaacag caacagcaac agctgttgaa gccttgaaag
     7321 ccattgctca acctgtatct ggcaaggaag ctattgctca ggtcgctgca gtatcatcac
     7381 gctctgaaaa agttggagag tatatctcag aagctatgga gcgtgtgggc aacgatggtg
     7441 tgattaccat cgaagaatct cgaggtatgg aaacagaact tgaagtggtt gaaggcatgc
     7501 aatttgaccg tggttacctg tctcaataca tggtcacaga caatgaaaaa atggttgcag
     7561 accttgaaaa cccatttatc ttaatcacgg ataaaaaagt gtcaaacatc caagacattt
     7621 tgccactact tgaggaagtt cttaaaacca accgtccatt actcattatt gcagatgatg
     7681 tggatggtga agcccttcca acccttgtct tgaacaagat tcgtggtact ttcaatgtgg
     7741 ttgctgtcaa agcgccagga tttggtgatc gccgtaaagc tatgcttgaa gacattgcta
     7801 tcttgacagg tggtacagtg attacagagg atctaggact tgaattaaaa gatgctacaa
     7861 tgacagccct tggacaggct gctaagatta cagttgataa agatagcaca gtaattgttg
     7921 aaggttcagg aagttcagaa gctattgcta accgtattgc actgattaaa tcgcaattag
     7981 aaacaacaac ttctgacttt gaccgtgaaa aactacaaga acgtttggcg aaattagctg
     8041 gtggtgtagc tgttatcaaa gtaggagctc caacagagac agctttaaaa gaaatgaaac
     8101 tccgcattga ggacgctcta aatgctacac gtgcagccgt tgaagaaggt atcgttgctg
     8161 gtggtggaac agcacttatt acggttattg aaaaagtagc agctcttgaa cttgagggcg
     8221 atgatgctac tggacgtaac attgtgcttc gtgctctaga agagcctgta cgtcaaattg
     8281 ctttaaatgc tgggtacgaa ggctccgtag ttattgacaa gttgaaaaat agccctgcag
     8341 gaacaggatt taatgctgca acaggtgagt gggttgatat gattaaaaca ggaatcattg
     8401 accctgtcaa agtaacacga tcagcgcttc aaaatgcagc ttctgtagct agtcttattt
     8461 tgacaacaga agcagttgtc gctaataaac ctgaaccagc agcgccagcg ccagcaatgc
     8521 cagcaggtat ggatccagga atgatgggcg gcttctaagc tctctatagc attaaggatt
     8581 atgaaaaaga agttgagact aaaggtctgc ttcttactat gaaaaaagaa caaagattgg
     8641 atttcgactc atgatatccc atctttgttt tttttagttg tgatataaaa tgccaatctt
     8701 tgtggttaca gacttttaat gtattggaaa atatattgtt tttctttgtc atgtgctatc
     8761 atggagtcaa tgaaaagtta ttcccatata tgcgtggtgg aggtctgtga tgaatcattt
     8821 tggagagatt tttaaaacct ttagagaatc aaggggatta cgattaaaag atgtcgcaaa
     8881 ggctggtata tcaacctctc agctgtcgcg tttcgaaaaa ggggagacgg atttaaccat
     8941 atcgacattt atgctgattt tggatgaaag taacatgtcc attgatgagt ttatgtatgc
     9001 tgtccatgat tttcatcgtg acgacacaac tcaagatagt gatggcttga agtagttact
     9061 tcatgctcaa ctagcatcaa ctgataagaa ggaaatgttc acggatatct tttggcatat
     9121 ccagcatgtc taagtctgct atggcatgcc attttgggag tccctcacgc gactctgtca
     9181 gtagctctcc ttcaaaggat tcacatataa agtcgtaata gacatagcac tctttttttg
     9241 tcaggcttga taaagctaga aatacccttt aattgaaggt ttaaagctgt cagacctatc
     9301 tcttctttta gttctcataa agccgcttca aagaaagatt cacagggctc gactttacct
     9361 tctggctggc cccatcctgg gaagttatcg tgttgtctat ggagtcataa aatcatatcg
     9421 ttatgtttga cacacatatt gccccagtct ctaaccgttg ctatcaccag attcaccacc
     9481 ttttagtata gtataagaaa aaaggggtga aagaggtttc ttgttgtttt tttggcttaa
     9541 gatttcttta agtcttatct agtatactaa tgtcattcct taataggata agtatttggt
     9601 tgtagaagta tcgtcttaaa ctaacgctat ctcaaccgat tcccatctta ttcacccctc
     9661 ctataccgat aaggtctcac aaagtgtgag accttatttt ttgacttcag ctctagttat
     9721 ggagaaactt gttgatacaa gcttttttgg gttatggtat tatgatctat tcaagccttt
     9781 cttgtttttg ggtttttctt caactaactt ggcttcaata gcttttaagc gtttgaaggt
     9841 tttttcagcg cgatctagtg aaatcttatc agctttttct tgagcagctt tggggtcact
     9901 gacgactaag tcactgattt ctttggtgct tttgtcttgt tcggctatcc aggttttttc
     9961 caagcctttc atctcatcaa tcactttagt tccaaatggt tttggatgag cagccacgag
    10021 atcattaatg tgtgacaccg tccaatacca tgacttatcg ttatattggg tgcttttttc
    10081 ttgataggct tcataggttc tactgatatt gccaaggtat ggcaagtaag gggcattacg
    10141 aggactgcca atagataacc acatgacacc accacccaat tcagcaggga tgtctttttt
    10201 cagttggaaa atatgagctt ccatgacatt aggattggaa ataggatacg catagccctt
    10261 aacagctttt ttggattttg gtttaccctt accgtctaat gccatctgat caagtggttt
    10321 aagatcaagc ccctcaaaac gattacgttg cagtttcatc gcatcttcta gactaaaggt
    10381 cttatcagtg ctttggagca actcataatt gctgtcttta taagttactt ttgaatcagg
    10441 gtcaagtgat ttaatccctg agaaagagcg agagcggttg gcatcgttta gtggcggatt
    10501 gtaagatttg gcaatatgaa atttaccatc aacttcggta taagatttgg cttttttagc
    10561 gactttttcc acatcttctg aggcaatcgt attttcctta tcgttgaaat cgacatgtcc
    10621 gagatagaag gtatttggaa aaacagcgta tttatcatct gggaatttga tggcaacgta
    10681 ttggtgacca gagagaattt ccatgtacca gatgccgtct ttgtcagcca aggtaacaat
    10741 atttccttca gcagctcctt tttcagtgac tattttagca atcaatgcga caccttcacg
    10801 ggctgttttg acacttggta aaatgacgct ggccatagat gattccgcta agccattttt
    10861 gacgtaagga tcaatttttt ggatggcatc attagctgaa gctgatactg ttgcagacat
    10921 agagatgcca aactcattaa aaccagcttc gtcataaacg cctttattag gtgttacatc
    10981 aggaatcgca ctatagcgat aagaatgctc aggcaatggg tactcaaaac cattagaggg
    11041 gtctttccat ttttcgccag caggattgtc ctttgctaga cgtacaatga agtttttatt
    11101 atgatgtggc tcaagatctt ctgttcgacc ataaagaaga gaaccgtctt tggttaaatc
    11161 tttgccaata ataaaaccag tacaagcata actaacactt tgaagcgaaa aggctgtcag
    11221 aatacttaaa actccaagtg atattttttt gtttatcatg cgaatcctcc tagtaaacga
    11281 taacgctttc acagacatta taaaatgctc tttttaaaaa ttcaagtctt ttatgaagtg
    11341 ttttgaaatt gttagctatc taaatattat gagaattatc tcttgaattt tggcttaacg
    11401 gcccctaaaa acgtataggc agtgaactat ctaggtttct tcgaaaatcc attagccatt
    11461 tatgatataa tagtaaagaa aaacatagaa agttggggtc tgttgatgac caaaccattt
    11521 catcataaga aactaaaaca aataactata atagctgcaa ctagcctttt tttattcctg
    11581 atcggtggtg ctttttacta ctctaaaaat cattgtatta atgcctatct taaagctcga
    11641 tcagcccaat caggaccagt ttttgagaat atcaaagcgt atctagtctg ggatgatact
    11701 aatgagcaga tcacaaatga cgaggcgatg tatactaagt ttagaaggta tagtcaaaaa
    11761 gaattgaggc aaaaaaagca ggatttaaaa gccgctagtc aagatagtgc cgttcaagta
    11821 aagtctgttg gtcgtcgttt ttggattttt cctgattatc ggattgccat aaaacccatg
    11881 gatttaacga taaaaacgaa tgtacctcaa gcagacgttc ttttaaatca taaaaaagtt
    11941 gctgtttctg attcagaaca gttctcagtc aagcttgatc ggctaccaac ggcagaatat
    12001 accgcaagta tcagaggcaa acacaacggg cgaaacatta aagtcaacaa atcatatgat
    12061 ggtgataatc ccgtgctaga tttgagtgtg tcttttagaa cttttttggt aacaagcaat
    12121 gctaagcaag gagatcttta cttcgatgat aaccatattg gcacattaaa agatggtcaa
    12181 ttacaagtag aagattaccc tgttacagaa aacgcacaag cttatatgaa aacaacattc
    12241 ccagatggtg agttaagatc acaaaaatat gctctagctg atgttgaaga aggagcaacc
    12301 ctggagattt tagttacaga tcttttagaa gaggacaagg caggggagct attagtatca
    12361 gcttttgatc agctgatgca ctacctcagt acaggtcaag actcatctaa cttacgtagc
    12421 gtctttgagg cagggtctag caacgcattt tatagaggat tgaaagagtc cataaaagcg
    12481 aaatttcaga cagatacaag aaaagccagc cgtctcaata ttccatctat ccttttgaca
    12541 acaatgactc aagtgggtaa aacaacttac gtgcttgatt tcacagctac ctacgaattt
    12601 ttgtatgaca actcaacaga tcctgagcag catacctctg gacatattaa tcaagacttg
    12661 attggaaaag tgactgtaaa aaaagttgga cagcattacc ttatcagcca gtccggctcc
    12721 aaaaatatta ctgttgttaa agaggacaat caactcaaag cgccatctgt ctttcctgag
    12781 tctattttgg gaacgtggac aggccaagcc aatggtttga gcatccatat gtctctagca
    12841 tcagatggaa caattacgac taaagttgaa gatcaaaaag gcaaccgttc taaagaaact
    12901 cggacagcta aaattagtaa agttgaagac aaaggcaatg gtttttatct ttatatgcca
    12961 gatcctggaa gtgacataag cgccttagtt ccagaaggag gattgggggg tgcaaatgtc
    13021 aaatatgctt atggtttcaa aatatctggt aaaacagcct ctccagtggt gtggcaggca
    13081 gcattaacac atgaatttga ttataccaag ccactttcgg gagtaacttt gcaaaagcaa
    13141 ccataaagga tgcaaagcca caaagaacag tagaaacaat gttatcactc aaatacagtg
    13201 ctatgaaaac agataaactc aaaaggctag gagattattt cctagccttt tgagcgttta
    13261 agactgcgtc aaatgtagct gatttagctt gcacgcaaat gtcttcttgg gttagagggt
    13321 ttttcaaacg taattgataa gcatggagca tcagtcgagc acaatctttt tttccattag
    13381 aatacaaagg atcaccaaat aaaacatgac cttgatgggc taaatggact cgaatttgat
    13441 gagtccgtcc agtttgtaat tgacaattga gtaagcttgc cgttttgtgg aagtttttga
    13501 ctagagttac ctcagtaata gctttcttgc catttatcgg atcgacaaca cgttttctgc
    13561 ggtcatgtcg atgacgacca attggatgat ggtaagttac cctaggactg tctaaggatc
    13621 cgtgaactag tgccaaatat tctcgatgga tatctcgttt ttctaaaaga cgattaagga
    13681 ttggtaagat aaaaggtgtt ttagcaaata aaatggcccc acttgtttcc ttatccagac
    13741 gatgaaccac atagcaggtc tgtcccgtgt aagcagacac gtggttgagc agcgccaact
    13801 ctgttggatc attgccgtgt gttttcatcc cttcaggttt gttaacaata atgatgtgct
    13861 catcttcata aaggcaggtt actttttcgg cctgccccat cacgataatc ttttcaggat
    13921 aatcttcgtg atcaaaaaaa agtttgacct gatcaccgta tttaacacaa ctttgccagt
    13981 taacaggatg accattaata agaacatgtt ttttagttct cagaaaatgc ctaattttac
    14041 ggggaattaa caattgttcc tctaataatg ctttgaccgt caattcttca aagggatttt
    14101 tcagtgtgac agtaaacccc tttgatggtt tatttgttgt cttcattgag ccagttatgg
    14161 tatttttcaa caaggttgat agccatttgg gtggcaagag cagcagagaa agcttcagta
    14221 ccttcacgct cgtcattagc tacctccaag cgagtctcgt cgtaaatggc ttttagactt
    14281 tctgcggata aggtttcttt aaaagcttgg aatttattca ttggttaaac ccgtccttct
    14341 atgtttaatg tgagctcctg attaatgcaa ggaattcaat gctattatag catgtttcta
    14401 atcaaaacgt accactcaaa aaggtaaaaa cgttattttg ctaatccata taataagaaa
    14461 gtgtcttaaa taagacctct tgatagagtt tgtaacaaaa atgatatgaa actaggtgct
    14521 aacgcagaaa tagaaaccaa ctaacgtgtt gcgaagttgg aacagtcata aaaaatagca
    14581 agataaaact tatcaacaca cagttaccta agacgatgaa tcaaacaggt atgttctttc
    14641 aaaacaaccc catgaggttg aacgacgtat cggaaaaata cggattagtt tggccttatg
    14701 ataggtattt ctctatcgta aaagaagtca accaaaggtt tttaatataa aatattaaga
    14761 aaaattgtta taattatgat aaaaacataa tcgtaaaata gaggaaaaac aatgaaaaca
    14821 attagacgtt atgatgttaa cgaagataga gggcatacag gccttgtaga agcaggagat
    14881 ttctattatt taaactattg tgtcggcaat gttggacaag acattgaaag tcagattaat
    14941 ggagcctttg atgagatgga gcgccgatta gcattggtgg ggctcacatt agatgcagtt
    15001 gttcaaatgg attgtctatt tcgagacgtt tggaacatac ctgtgatgga aaaaatgatt
    15061 aaagagcgct ttaatggcag atatcctgct cgaaaatcga ttcaaacgga atttgcacat
    15121 cacggtggac cacaaggact actctttcaa gtggatggtg tggcttattc aaagcatatt
    15181 tcgatgacct aatcaaggtt actttaagat catagtggta taataacgtt atgagatttc
    15241 tagaactttt acaaaagaaa ttttttccta aagcatatca ggaaaaacaa ttcttaatgc
    15301 atcaaaaaac gcgtttaacg ccacaacaca atcaaaagca gtattcgcca aatgccaatc
    15361 atttggactc atcagctacc aaaaactcag aacaagaccc tgcaatagct ctgcaacgca
    15421 gtagagccta tgaaggaagc cctaaaagtc ggcccgcttg gttgcaaaag ctggaagctg
    15481 ttttgccgtc tcctcaacat ccaattcggc gtttttggcg ccgctatcac atcggaaaac
    15541 tgctaatgat tctgattgga actcttgtct tactcttagg atcatacttg ttttacttat
    15601 caaaaacagc taaagtatct gatttacaag atgccttgaa ggctacaact gttatttatg
    15661 atcacaaagg agagtatgca ggcagtttat ctggtcaaaa agggagttat gttgagctca
    15721 atgctatttc agatgatctt gagaatgctg ttattgccac tgaggatagg actttttaca
    15781 gtaatagcgg tattaatctt aaacgcttct tattggcagt agttacggcg ggccgctttg
    15841 gaggtggctc aacgattaca cagcaactgg ctaaaaatgc ttatctctca caagatcaga
    15901 caattaaacg aaaggccaga gagttttttt tggcgttaga gttgaccaaa aaatacagta
    15961 aaaaagatat tcttactatg taccttaaca actcctattt tggaaatgga gtttggggag
    16021 ttgaagatgc cagtcaaaaa tattttggaa ccacagctgc taacttaaca ttggatgaag
    16081 ctgccacatt agcaggtatg ctcaaaggac ctgaaatata taacccttac cattctctaa
    16141 aaaatgctac tcaccgtaga gatactgttt taggagcgat ggttgatgcc aaaaagatta
    16201 cccaaacaaa agctcaacaa gctagagcag tagggctaaa aaatcgctta gctgatactt
    16261 atgttggtaa gacagatgac tacaaatacc catcctactt tgatgctgtt attagtgaag
    16321 caatagcaac ttatggtctt tcagaaaaag acattgttaa taatggatac aaagtttaca
    16381 ccgagctaga tcaaaattac caaactggca tgcagacgac ttttaacaac gatgaactat
    16441 ttcctgtttc agcttatgac ggtagctctg ctcaagcagc tagtgttgct ttagatccta
    16501 aaacaggagg tgttagaggt ctgattggtc gtgtgaatag tagtgaaaat ccgactttca
    16561 gaagttttaa ctatgcgact caagcaaaac gtagtcccgc atcaacaatc aaaccactcg
    16621 tggtttacgc gccagccgtt gcttcaggat ggtcaattga aaaagaacta ccaaataccg
    16681 ttcaagattt cgatggctat cagccacata attatggaaa ttatgaatca gaagatgttc
    16741 ctatgtatca agcattagca aactcttata atattccagc agtttctaca ttgaacgata
    16801 tcggaatcga taaagccttt acctatggta aaacatttgg gttagatatg agctctgcca
    16861 aaaaagagtt gggggtagct ttaggtggca gcgtgacaac caatccattg gagatggctc
    16921 aggcatatgc tgcctttgcc aataatggag taatccatcc tgcgcacttg attaaccgga
    16981 ttgaaaatgc caggggtgaa gtgcttaaaa cctttactga taaggctaaa cgtgttgtca
    17041 gccagtctgt tgcagataag atgacagcca tgatgctagg taccttttca aatggaacag
    17101 cagtcaatgc taacgtatat ggctatacac tagctggtaa aacagggacg acagaaacca
    17161 acttcaatcc cgacttagca ggcgatcagt gggttattgg ttatacgcca gatgttgtta
    17221 ttagtcaatg ggtaggattt aatcagaccg atgaaaatca ttatctaacg gattcaagtg
    17281 caggcacggc ttcagctatt tttagcactc aggcatctta cattttgcct tataccaagg
    17341 gcagccaatt tcatgtagat aatgcctacg ctcaaaatgg tatttcagct gtttatggag
    17401 tcaatgaaac aggtaatcaa tcaggagttg acactcaatc tattattgat ggtttaagaa
    17461 aatcagcaca agaagcttcg caatcactat caaaagcagt cgatcagtca gggttacgtg
    17521 ataaagccca atctatttgg aaagagattg ttgactattt tagatagctt gcagtatttt
    17581 agagatcgtg ttaaaataat agttacggag gcgttatggc acagaaaaaa gcgagtctag
    17641 cgtgtgtcga atgtggcagt cgcaactact ctatcggagt gagtagcacg cctaaaccaa
    17701 cacgtctaga agtaaataaa ttttgcaaat actgcaaaac atatacctta cacaaagaaa
    17761 cacgttaaaa aggagagcct gatgggattc attagcggga cttttaaagt cttaaaagac
    17821 acaacatggc caaatcgtaa acaacgttgg aaagatttta tctcagttct tgagtacaca
    17881 gctttcttta cggtgattat ctacatcttt gaccagctct tagcaaagag tgtattagca
    17941 ctgattaatt tattttaggt caagattggt agaaatgata agcttgcttg gaaaagccta
    18001 ggctgggagt attcccagcc ttttttgtag tatcaataat ctgattagta agctttttta
    18061 aagcccatac tggtttgagc tattcaaaag cataaaggta aaggcaatgt agcaacgatc
    18121 tccaaaccaa gttcactaaa ttggcttgta acagagtcat cgcataaaca gcctggcctc
    18181 acttgcttag cacattaaaa atagcaatga atattatggt tgaggaaaac agcctgatag
    18241 tctgctaaat cttcgggatg ataggccttg acctcagcca tctcatactc cctacctaga
    18301 agtttgtact tgttttctcc aaattgatgg aaaggtaaca gttgcacttg gtcaatctca
    18361 agctgattaa agagctctga gaaggcttta gcatcgtcta gggaatcatt aaattgggga
    18421 ataacaggga tgcgcaagac gatttctttt ccagcttgaa aggcataatg aatatttttg
    18481 ataatcaagt cattacgaac cccagtcact ttttggtgtc tgagctgatt ataatgtttc
    18541 aaatcggtat agataaagtc cacgtagtca ataagggtga caaattgctc gtgtttggca
    18601 aaggcggtgg tttcaattgc ggtgtgaaga cctgctgctt tggctgcttt taacagggct
    18661 aaggcaaagt caaactgagc aaagatttcc ccccctgaca gggtcatgcc cccacctgac
    18721 tcttcgtaga aatcaaggtc tttaaggact tcttcgatca cctcgtccac ggttttttcc
    18781 tctccaacta ttttagtatt aagcccatca cttgttaaca tctgctcagg cgctttttgt
    18841 tgggattcgg gattggcaca ccaaggacat ctcaaggggc aaccttttaa aaaaacggtt
    18901 gtccgaatac cagggccgtc atgaatacta aaatgttgga tgttgaaaac aataccgcga
    18961 tcagtcataa gtgtgaattc ctttcgttgt aaaaccatca taccagataa acgtaaataa
    19021 atcaattacg aatgaaagta aatatccctt ttcttttaag aaaataactg gtacaatgaa
    19081 agagaggtga atgatgaatc gattagaacg tattattcaa ctggtttccc agaagaaaaa
    19141 aatagatgtt aacagtttat cagagcaatt agacgtttcg aaagtaacca ttcgaaaaga
    19201 ccttgataag cttgaaagta agggcttgtt gcgacgggaa catggctatg cggtgctaaa
    19261 cagtggcgat gacctcaatg tacgcttgtc ctacaattac aacataaagc gacggattgc
    19321 agaaaaagca gctgaattgg tgcaagacaa cgataccatt atgattgaat ctggctcaac
    19381 ctgtgccctt ttggcagaag tgctttgcca gaccaaacga aatattaaaa tcattaccaa
    19441 ttcgtgcttc attgccaact acattcgtca atatagtagc tgtcaaatca ttttactggg
    19501 cggttattac cagcccaatt cagaggtgac tgttggtcct ttattgaaag aaatgatatc
    19561 cctttttcat gtcaatcgtg tctttgttgg aacagacggt ttcaacaaag atcttggctt
    19621 tatgggcaag gacatgatgc gctcagaagg ggtgcgttac atggcagatg ctgctgagga
    19681 agtggtgatt ctgaccgatt ccagcaagtt ttcaaaaacc agtctggtac accagttgtc
    19741 attagctgat gtcaatcgcg tcattacaga ccaagcacta gacaagcaaa cacaagagtt
    19801 attaagtgct agtggtcttg ttcttgattt tgtttcttag aaaataggtg gctagatgaa
    19861 agaagaacgc agacggctcc ttgccaaggt ggcttacttg cattatgttc aaggcaagag
    19921 ccaaaccttg atttcaaagg aaatgaatat ctaccgaaca acggtttgtc ggatgttagc
    19981 aaaagccaag gaagaaggca ttgttcggat tgaaattgcc gactatgatg ctgatttatt
    20041 tgctttagaa gaatacgtgc gacagcagta tggcttggaa aaactagacc ttgtgcccaa
    20101 ccaggttgaa gataccccaa tggatacctt gacaaatgtt gctaaaacag cagctgaggt
    20161 ttttagacat gttgttaaag atggcgataa gatcggtctg tcctggggag cgactctttc
    20221 ttgcttgatg gatgagttaa atccaaaagc gatgaaagat gtgtttattt atcctttggc
    20281 aggaggacct agccatatta atgccaaata ccatgtcaat accctggttt accgcttggc
    20341 tcgcatcttt catggtaata gcgcttttat gaacgctatg gtcattcaag aagacaaaca
    20401 cttggctaag ggaatcttac aatctaaata ctttaacgac attttaacaa gctgggacca
    20461 gttagatctt gccttagtag gaattggtgg ggagcctaat agccttgagc agagccaatg
    20521 gcgtgattta ttaaccagct cagatcatga ccaactcaaa tatgaaaaag ccgttggtga
    20581 agtttgttgc cgtttctttg atcaggcagg tcagccagtt tacactggcc tacaggatcg
    20641 aactattggt atttcactag aacagttgag gcgtgtcccc aaaacaatgg ctgttgcaac
    20701 aggtaaacac aaggctaagg caatattggc agccttaaaa gcaggtttta tcaattatct
    20761 agtgacggat aaagagacca tgctagctgt tttagcgtta gatgaagata ttgatcttaa
    20821 taacgttctt ctttaataga aattcaaaaa tactatgata tttatagagg cccaagacca
    20881 aacggtcagg gctttttttg tttgatgtgt tgataaaaca gagattgctc aaatgtgcag
    20941 ttaatgtatt ggcacaaaag tgcattaacc aaaatatagc acaaaatata ttgaccctaa
    21001 aaacaaaaat gatacaataa acttaccaac gaaatgtaaa cgattacaaa cacaataaca
    21061 tcaccttcga aaaaaagact cattgccgtt acatcacatg aaaaagaaaa aaaggagaca
    21121 ccaatgcaag ttattgtacc agaccaaatc attatgggct taattttaaa tgcaggcgat
    21181 gccaaacaac atatctacca agctctgaaa tgtgctaaag aggacgatta tgccactagt
    21241 gaaaaagaaa tggccttagc agatgatgcc ttattagaag cccacaattt acagacccag
    21301 tttcttgccc aagaagctag tggtaataag tcagagatta cagctctttt tgttcactca
    21361 caggatcact tgatgacgac cattacagaa attaatctca tcaaagagat tattgacctt
    21421 cgtaaagaat tggcaactaa ataagttttc atgactaaaa aggagattga catgattaaa
    21481 attggattgt tttgtgcagc aggattttca accggtatgt tggtgaacaa catgaaagta
    21541 gctgctgaaa aaaaaggcat tgattgtcag attgaagcct atgctcaagg gaaattagcg
    21601 gactatgcac cattacttga tgtggcactt ttaggcccac aggttgccta tactctggat
    21661 aaatcagaag ctatttgtaa agacaatgat atccctattg cagttatccc aatggctgat
    21721 tatggaatgt tagacggcaa caaggtgctt gatttagccc ttagccttgt taaagaatag
    21781 gagtcaatca tggcaaaaat gaatatgcaa aacatcatca agccaatcat gacatttgtg
    21841 aatatgcgcg gcatcattgc cttaaaagac ggtatgttag ccattttacc tttgaccgtt
    21901 gtggggagtc ttttcctgat tgctgggcag attcctttcc aaggggttaa tgatgccatt
    21961 gctagtgtgt ttggtgctga ctggacagaa ccctttatgc aggtttacca tggaacattt
    22021 gctattatgg ggttgatttc ctgttttgca attggctatt cttatgctaa aaactcagat
    22081 gttgaacctc tcccttcagg ggttctatcc ttgtcagcct tctttatttt attgagatca
    22141 tcttatgttc cagcagaagg tgaagctatt ggagatgcta tcagtaaggt ttggtttggt
    22201 ggtcaaggga ttattggtgc tattgtgatt ggtttaacgg tcggagctgt ttacacaaca
    22261 tttatccgtc gtcatatcgt cattaaaatg ccagatcaag tgccacaagc catcgccaaa
    22321 cagtttgaag ccatgattcc agcctttgtg atctttacct tgtcaatgct tgtgtatatt
    22381 attgccaagt cagtaacagg tggtggcacc tttattgaaa tgatttatga tgtcattcag
    22441 gtaccgctgc aagggttaac gggttctctt tatggcgcac taggtattgc cttctttatt
    22501 tcctttcttt ggtggtttgg cgtgcatggg caatctgtgg tcaatggaat tgtcactgct
    22561 cttctcttat caaatctaga tgccaataag gccttgatgg cagcaggtga gttatcctta
    22621 gacaagggtg cccatattgt aacccaacaa tttttagact ctttcttgat tctatcaggt
    22681 tctggcatta cctttggttt agtggtagct atgatctttg cggctaaatc caaacaatac
    22741 aaggctttag gtaaggttgc agccttccca gccctcttta atgttaatga accagttgtc
    22801 tttggttttc caattgtgat gaatccagtc atgttcttgc catttatctt ggttcctgtc
    22861 ttggcagctc tcacagttta tggcgccatt gctattggtt tcatgcagcc ctttgcagga
    22921 gtgacccttc cgtggtcaac accggccatt gtctcagggt tcatggttgg tggctggcaa
    22981 ggagctattg tgcaaattct tatactcatc atgtcaacgt tggtgtactt cccattcttt
    23041 aaaattcaag ataatatggc ttatcaaaat gaacaagcta gtgaagagtc atgatttagc
    23101 gtccacacaa attcttgaga ccatctccag ttagaggtgg ttttttagaa aaagaagagc
    23161 gacttatcag gcctatattg cacaatagtg caaaataaag tgactatctc ttcttatggt
    23221 taaggatacg caaaagtcgc aatatatgat aaaatagtta cgaaagaaac atttataatt
    23281 tcgtaagaaa gaaggagacc ccgcatgact gaaacaaaaa gcccttattt tggacattta
    23341 acagacagaa tgactcacta tcgtgaagct gtgttagaca agaaacctta tattgatgct
    23401 gagcgtgcta ttttagccac tgaagcctat caaaaacacc aaaataagcc agcaaacctc
    23461 aaacgcgctt acatgcttca aactattttg gaaaacatga ccatctatat tgaggatgaa
    23521 agcctcattg ctggtaacca agcctcttcg aacaaggatg cgccgatttt cccagaatac
    23581 actctggaat ttgccctcaa cgaacttgat ctttttgaaa aacgagatgg ggatgtcttc
    23641 tacattaccg aagaaaccaa gcaacagctc cgagacattg ccccattctg ggaaaacaat
    23701 aacttacgtg ctcgctgcgg ggtcttgcta ccagaagaag tgcaagtgta catggaaaca
    23761 ggtttctttg gcatggaagg caagatgaac tctggggatg cccatttagc ggttaattac
    23821 caaaaactct tggagcacgg cttaaaggga tttgaagaac gggcacgagc tgccaaagct
    23881 gcgcttgatt tgaccattcc tgaaaacatt gacaaatacc atttttacga ctctgttttt
    23941 attgtcattg atgcagtcaa aacttatgcc aaacgctatg ccaagttggc tagagaacta
    24001 gcaaaaacgg ctaaaccaga acgtcaagca gaattgttag acatcgcgcg tatttgtgac
    24061 aaagtgcctt acgaaccagc gaaaaccttt gcagaagctg tgcaatctgt ctggttcatt
    24121 cagtgtatct tgcaaattga atcaaacggg cattccctat cttatggccg ctttgaccag
    24181 tacatgtatc cttatgtcaa agctgatttg gaggctggcc gtgaaacaga agactcaatc
    24241 gttgaacgtt tgactaacct ctggattaag accttgacca ttaacaaagt gcggagccaa
    24301 gcccacacct tctcatcagc aggtagtcca ctttaccaaa acgtgacgat cggtggtcaa
    24361 acacgcgata aaaaagatgc ggtcaaccca ctgtcttacc ttgtcctacg gagtgtggct
    24421 caaaccaaat tgccacaacc taacttgact gttcgttacc acaaaggcct agacaatacc
    24481 tttatgaacg agtgtatcga ggtcatgaaa cttggttttg gcatgccagc catgaacaac
    24541 gatgaaatca tcattccatc tttcatcaaa aaaggtgtct cagaagaaga tgcttatgat
    24601 tactcagcca ttggctgtgt ggaaacagcg gttccaggca aatggggcta ccgttgcacg
    24661 gggatgagtt acatcaactt ccctaaaatc ttgctgatta ccatgaatga cgggattgac
    24721 ccagcatcag gcaagcgctt tgcaaaagga tacggtcact tcaaagacat gacctcttat
    24781 gaagaattaa aagcagcatg ggatgccacc ttgcgcgaaa ttacccgcat gagcgtcatt
    24841 gttgaaaatg ccattgacct aggtcttgaa cgtgaagtgc cagatattct ttgctcagcc
    24901 ttgacagatg attgtatcgg tcgtggcaag accttaaaag aaggcggagc tgtttacgac
    24961 tatatctcag gccttcaagt tggaattgcc aacctatcag actccctagc agcgctgaaa
    25021 aaattagtgt ttgaagaagg ccgcttgacc ccagaagaac tctggcaggc ccttgaaagt
    25081 gactttgctg gagaacgcgg agaagaaatc cgccaaatgc tcatcaatga cgcaccaaaa
    25141 tatggtaacg atgatgacta cgcagatagt ttagttgtcg aagcttacga cacctacatt
    25201 gatgaaattg ccaaataccc aaatacccgg tatggccgtg gcccaatcgg aggtatccgc
    25261 tattctggga catcatcaat ctcagccaat gtcggacaag gaaaaggaac cttagcgaca
    25321 ccagacggtc gccatgcagg cacaccacta gcagaaggct gttcaccaga acacagcatg
    25381 gacaaaaaag gacctacttc agtattgaaa tctgttgcta aattaccaac agatgagatt
    25441 gtcggaggag tactactcaa ccaaaaagtt aacccacaaa ccctggccaa agaagaagac
    25501 aaactgaaat taatggcctt acttcgtacc ttctttaatc gcctacacgg ttatcatatc
    25561 cagtacaatg tcgtgtcacg tgaaaccttg attgatgccc aaaaacatcc tgaaaagcac
    25621 cgcgacctca ttgtccgtgt tgccggctac tcagctttct tcaatgtgct ctcaaaagca
    25681 acccaagatg acattattga acgtacagaa cacacacttt aggagaaaat aagtatggaa
    25741 tacatgttag acactttaga tttagaagct attaaaaaat ggcatcacat tttgcctctc
    25801 gcaggtgtga cctcaaaccc ttctattgcc aaaaaagaag gtgacattga tttctttgaa
    25861 cggattcgag aagtccgtgc tattattggt gacaaggctt ctattcatgt tcaagtgatc
    25921 gcccaagatt acgaaggcat cttaaaagat gctgctgaaa tccgcaggca atgtggagat
    25981 agtgtgtatg tcaaagtacc tgtaaccaca gaaggccttg cagccattaa aaccttgaaa
    26041 gccgaaggct atcatatcac agcaaccgct atttatacga ccttccaagg gttattagcc
    26101 attgaagctg gcgctgatta tttggctcct tactataacc gtatggaaaa tctcaacatt
    26161 gatccagaag ctgtcatagg acaattagca gaagctatta accgtgagaa tgctaatagt
    26221 aagattttgg cagcaagctt taaaaatgtg gctcaggtca ataaatcctt tgctctagga
    26281 gcacaagcca tcactgctgg cccagatgtg tttgaagcag gatttgccat gccatctatt
    26341 caaaaagcgg ttgacgattt tggcaaggat tgggaagcca ttcatcaccg caagagcatt
    26401 taagaaatag catcagtagt aatcacatca ttaggaatca cagataaagg agttcaacga
    26461 tgaaagtatt tgcaagtcca tctcgttaca ttcaaggtaa aaatgccttg tttaccaatg
    26521 ctaaaaccct aaaacaacta ggggatagcc ccattttact gtgtgatgac gtggtgtatg
    26581 gcatcgtcgg agaaagattt gaaagctatt tgatagataa tggcatgact cctgttcacg
    26641 tagcctttaa tggcgaagcc tcagataatg aaatcagccg cgtggttgcc attgccaagg
    26701 aaaatggcaa tgatgttatt attggacttg gtggcggaaa aaccattgac agtgctaagg
    26761 ctatcgctga cttacttgct gttccggtga ttattgcccc aaccattgct tcaacagatg
    26821 ccccaacctc agccttatcg gttatttaca ccgatgaagg agcctttgaa aaatacattt
    26881 tctattcaaa aaatccagat cttgttttgg ttgacacaca agtgatctgt caggcaccaa
    26941 aacgattact ggcctctggg attgcagatg ggttagcaac atgggtagag gcgcgtgctg
    27001 ttatgcaaaa aaatggagac accatggcag gtggcaatca aaccttggca ggagttgcca
    27061 ttgccaaagc ctgtgagcag accttgtttg cagatggcct aaaagcgatg gctagctgtg
    27121 acagacaagt cgtgaccccg gccttggaaa atgtcattga agccaataca ctccttagtg
    27181 ggcttggttt tgaaagtgcg ggtctggctg ctgcacatgc cattcacaat ggctttacag
    27241 cgctgacagg tgctattcat caccttaccc atggtgaaaa agtcgcctat ggcacgttga
    27301 cccaactctt cttggaaaat cgttcgcgtg aagaaattga ccgctacatc gacttttacc
    27361 aagctattgg catgccaaca accctcaaag aaatgcattt agacacggct actcaagagg
    27421 atttcttaaa aattggccgt caagcaacca tgacaggcga aaccatccat caaatgccat
    27481 ttgttatcag tccagaagat gtagcagcgg ctttggttgc tgttgatgcc tatgttacga
    27541 gtcgataggc taaccaaaaa gagatttctt atttgccaga taacctcaaa aaaacaagtc
    27601 tagggagtca cttcctagat ttgttttgct tattgttggt tggtaatagc acttttagcg
    27661 gggtcattgt gggcaatacg gctggcagct gcagcagcaa tggcgctaac aatatcatct
    27721 aaaaaggtat ggcaggagta gccatcttta tggttgagtt tgtcaacaat tcctggtttt
    27781 gtcttgtcca gatacccata attggtaaaa ccgatagacc catagaggtt aacaatggat
    27841 aaggccagga tttcatcaat cccatacagc ccttggtcgg ttttcaagat actaaggagt
    27901 ggctcagaca gctggttggc ttcagccaat ttatctaatt ctaccccagt gataatagcg
    27961 ttttgcactt ctcgttttgc aagcaccgcc tcaacgctct ctaaacattc tgccatggtc
    28021 aaattgggaa tataatcatt ctgcaaaaaa agcaccagct ctgcaatagc ttctaaagaa
    28081 ccccctcgct cagccagtaa ttgataagac acttctcgta ggtgatggtc agttgacatg
    28141 tgagacctcc tttttttctt tattgtaaca taaaagattt cgagaaacct ctacaacagc
    28201 taaagagggg atagttcttt tttaagagat gttgactact gagatcccct acttggtaaa
    28261 agtcaaagaa acgatagtaa agtaaccatc ttacctaggc ctaaatacaa tcaccacccc
    28321 taggtgacac acagcaccta tttcaaaaat gatattagta tggcaaaaca aaaagacaac
    28381 gccttctttt ttctccttac tatctccttt aattttcata ttttttaaaa aaactattga
    28441 taaactagtt aagtaagcgt atactatggt tagtgagcga aattagaaaa gaggacaagc
    28501 atatgaatct acttggatca agacgggttt tttctaaaaa atgtcggcta gtaaaatttt
    28561 caatggtagc tcttgtatca gccacaatgg ctgtaacaac agtcacactt gaaaatactg
    28621 cactggcacg acaaacacag gtctcaaatg atgttgttct aaatgatggc gcaagcaagt
    28681 acctaaacga agcattagct tggacattca atgacagtcc caactattac aaaaccttag
    28741 gtactagtca gatcactcca gcactctttc ctaaagcagg agatattctc tatagcaaat
    28801 tagatgagtt aggaaggacg cgtactgcta gaggtacatt gacttatgcc aatgttgaag
    28861 gtagctacgg tgttagacaa tctttcggta aaaatcaaaa ccccgcaggc tggactggaa
    28921 accctaatca tgtcaaatat aaaattgaat ggttaaatgg tctatcttat gtcggagatt
    28981 tctggaatag aagtcatctc attgcagata gtctcggtgg agatgcactc agagtcaatg
    29041 ccgttacagg aacacgtacc caaaatgtag gaggtcgtga ccaaaaaggc ggcatgcgct
    29101 ataccgaaca aagagctcaa gaatggttag aagcaaatcg tgatggctat ctttattatg
    29161 aagccgctcc aatctacaac gcagacgagt tgattccaag agctgtcgtg gtatcaatgc
    29221 aatcttctga taataccatc aacgagaaag tattagttta caacacagct aatggctaca
    29281 ccattaacta ccataacggt acacctactc agaaataata ccaaaaggct agacctctgc
    29341 tcactaggcc tagcttttta catcaaaaaa agcaatgact atagaaagta aaaatactag
    29401 aaaaagcaat gattgccgtc attgcttttt atgatttgtc aaaaagtaaa aagcaaattg
    29461 aaaaaatcgc cctggagctg ttgagataaa ctacaccatt tattaagata ccaaatc
//
LOCUS       DAUCLR010000013        29162 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.013, whole genome shotgun sequence.
ACCESSION   DAUCLR010000013 DAUCLR010000000
VERSION     DAUCLR010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29162
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.013"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            43..1341
                     /gene="rocA"
                     /locus_tag="VGT87_000463"
     CDS             43..1341
                     /gene="rocA"
                     /locus_tag="VGT87_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RocA"
                     /protein_id="HEQ3813348.1"
                     /translation="MLTLKLFSFVSAIPLRLKNIFYLSLSMVLFQVVFWAFFPDHFIL
                     DVVMLAQFLFFALIALYYGKSIKAKFLMFYAFFPLVSISLVKRFIVFFVMPLFGMPYS
                     VVKHNTLLIYSITCFSIFLIYRCIQVFHFDFSTWRQYFQSHRASKLLVFTNSSMALYY
                     LCVQGIDVMSPSLSGLATTTARSIIVLFYFILFLTLLIHLERYVKQNSIEAIVQQKEY
                     RELINYSQHLGLLYQDIQELRQLLTTVSSRLKIGIEQNDISIVRLTYEGILNAEKNNA
                     KDDRLDLTCLDKLQVEAIRHIVLAKLIEAKNKKLKVEVSIPNCIATFFLEVVDLTKLL
                     SFLLDNAIEMSLETKQPCLSIAFLDQNHKLVIVIQSSTKQGQDDSQSVFAIPALKKRD
                     DWQFDLRNVTTILNRYDYLTISSQIHDGILTQLIEIAKPD"
     gene            complement(1442..4147)
                     /locus_tag="VGT87_000464"
     CDS             complement(1442..4147)
                     /locus_tag="VGT87_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEQ3813349.1"
                     /translation="MATKKVHIISHSHWDREWYMAYEQHHMRLINLIDDLLEVFQTDP
                     DFHSFHLDGQTIILDDYLKVRPEREPEIRQAIASGKLRIGPFYILQDDFLTSSESNVR
                     NILIGKEDCDRWGASVPLGYFPDTFGNMGQTPQLMLKAGLQAAAFGRGIRPTGFNNQV
                     DTREKYSSQFSEISWQGPDDSHILGLLFANWYSNGNEIPTTEAEARLFWDKKLADAER
                     FASTKHLLMMNGCDHQPVQLDVTKAIALANQLYPDYEFVHSCFEDYLADLADDLPENL
                     STVQGEITSQETDGWYTLTNTASARIYLKQANTRVSRQLENITEPLATMAYDVTGTYP
                     HDQLRYAWKTLMQNHPHDSICGCSVDSVHREMMTRFEKAYEVGHYLAKEAAKQIADAI
                     DTSDFPTDSQPFLLFNTSGHSKTSVTELTLTWKKYYFGQRFPKEVYQEAQEYLAELSQ
                     SFQVIDVSGNTISEADILDTSIAFDYDLSKRTFREPYFAIKVRLRLPITLPAMSWKAL
                     ALQLGNETAPSATVPLYDDSNQCLENEFLKVMIQTDGRLTITDKQSGLIYQDLLRFED
                     CGDIGNEYISRQPNHDQPFYADQGTTKLNIISNTAQVAELEIQQTFAIPVSADKLLQA
                     EMEAVIDITERQSGRSQEKAELTLTTLIRMEKNNPRLQFTTSFDNQMTNHRLRVLFPT
                     HLKTDHHLADSIFETVKRPNHPNAAFWKNPSNPQHQECFVSLFDGEKGVTIGNYGLNE
                     YEILPDTNTIAITLLRSVGEMGDWGYFPTPEAQCLGKHSLSYSFESITKQTQFASYWR
                     AQEGQVPILVEQTSHHKGSLRAEHSYVTGADDQVALTAFKRRLADNALITRSYNLSND
                     KTCDFSLSLPNYNAKVTNLLEKDSKQSTPSQLGKAEILTLAWKKQ"
     gene            complement(4162..5448)
                     /locus_tag="VGT87_000465"
     CDS             complement(4162..5448)
                     /locus_tag="VGT87_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEQ3813350.1"
                     /translation="MTRPIPTSVSQFMAKVESLCGDQHPDWALNFKTSFTNTLETTLK
                     TYEDGTSFLLTGDIPAMWLRDSTAQMKPYLFLAKEDEEIRKIIAGLVKRQFRYICIDP
                     YANAFNEEANGKGHQTDRTQMNPWIWERKYEIDSLCYPIQLAYLLYRETGSTDQFNDD
                     FHRGVELILDLWTVEQDHAQSPYLFERDTWRKEDTLTHAGKGSPVAPTGMTWSGFRPS
                     DDACQYGYLIPSNMFAVVVLSYLEDLYNNLFHNEPVATRAKQLKEAIQSGIADHALVQ
                     NSKGETIYAYEVDGLGQFSIMDDANIPSLLAAPYLGFCAKDDPIYLATRRTILSQENP
                     YYYQGNAAAGIGSSHTPENYIWHIALVLQGLTALDQDSKKEMLDLLVATDAGTHLMHE
                     GFDVNDPYQYTREWFSWANMMFCELLLDYLGFSIAP"
     gene            5581..6621
                     /locus_tag="VGT87_000466"
     CDS             5581..6621
                     /locus_tag="VGT87_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ3813351.1"
                     /translation="MTKLLYKKIYHFIKEQIASGRLQIGDRLPTEKELSEQFSVSRIT
                     SKRALVELEQEGLITRSRGKGSFVAEKQVTSPEANKDLLLILPFASDYELGDYAKGIM
                     TSIAETGYRLMVQLASTVRLDTLSDYAGIIYYPEDVNHSIDFLFYCDHHHIPLVLLDK
                     SLDLFQFPSVVADNKGGACQLTQHLIEQGCDQISFVATGSFGEVSSVRDRYLGYLAAM
                     AESSLKSHYFPKTETETTDAYLDRLVKTLSETTATKRGLVVENDWLAIQLIQKITQVG
                     LSIPDQVAIVGFDNSQASRLLSPKLSTAAQDFYQMGQEAAKVLLQIIHEPDQAVVSCQ
                     LPVQVFIRESSH"
     gene            6672..8366
                     /locus_tag="VGT87_000467"
     CDS             6672..8366
                     /locus_tag="VGT87_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase"
                     /protein_id="HEQ3813352.1"
                     /translation="MTIYHALKDYQEVMTRGDYLVFDTPLTCRFIGRFFRFENQKALL
                     AELATSKYFQWIEEGQAEVTMKHFFNRQLAKDAFTLKVSEDKEIIIESQNLRGFRYAQ
                     EALLKVMTFKGDKLYLPIVSVKHSPSFPMRGIIEGFYGTPWTREERIDCLRFIGNKRM
                     NTYMYAPKDDDYQRKLWRDLYPEDWVTYFKELLVVAKEEGIDFWYMISPGLDFDYTKE
                     ADYQLLYQKLQQLLALGVCHFGLLLDDIDYQIVDAVERRFKKTAYAQAHLATEVHHFL
                     NQQHAAPELVICPTEYDNHHDSIYLQELSERIPKEVAFFWTGPSTLASQISQADIETM
                     AAVYQRPIIIWDNIPVNDYQKDPERLFLTPFANRSPFLCQSDYQVKGIVSNPMISWEL
                     SKLTLTDMSHYLWDANRYQPSNSWLETLTDYTGDAELALALQAFAWHNGNRHLHRDLP
                     FEVEEALLAKDVSTLSAWVDELVERANTLRKLDKPAFQQAIVPWFERVAKDQDFWQAI
                     LEQGPHLETLYADLQEDKHRIGSDIPSRYYRIYYQQQDKLTANQGQVTQARPEDYA"
     gene            8391..9833
                     /locus_tag="VGT87_000468"
     CDS             8391..9833
                     /locus_tag="VGT87_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEQ3813353.1"
                     /translation="MLAFPKEFWWGGATSGPQSEGRFAKQHRNLFDYWYEEEPDLFYD
                     YVGPDTASDAYHQIESDLALLASLGHNSYRTSIQWTRLIDDFEQATINPDGLAYYNRV
                     IDACLANGIRPVINLHHFDLPIALYQVYGGWESKHVVDLFVAFSKVCFEQFGDRVKDW
                     FVHNEPMVVVEGSYLMQFHYPAIVDGKKAVQVAYNLALATAKVIQAYRRGPAELSDGR
                     IGTILNLTPAYPASQSEADTAAAHFAELWNNDLFMEAAVHGKFPEELVAVLKKDGVLW
                     QSTPEEMALIAENRVDYLGLNFYHPKRVKAPDAIPVISPSWSPEWYYDPYLMPGRRMN
                     VDKGWEIYPEAVYDIAIKMRDHYDNIPWFLSENGVGISGEDRYRDETGQIQDDYRIQF
                     LKEHLTYLHKGIEAGSNCFGYHVWTPIDGWSWLNAYKNRYGLVENNIHTQVRRPKASA
                     YWFKKVATHNRLISLEVMEEFGGSASHLSD"
     gene            complement(10444..11322)
                     /locus_tag="VGT87_000469"
     CDS             complement(10444..11322)
                     /locus_tag="VGT87_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEQ3813354.1"
                     /translation="MSLLCLDIGGTSLKFALCHNGQLSQQSSFPTPSSLEKFYQLLDQ
                     EVARYSAYHFSGIAISSPGAVNKEKGVIEGASAIPYIHHFKIQEALEERLHYPISIEN
                     DANCAALAEATLGAGKGASSLAMLVIGTGVGGSLVIDGKIYHGAHLFGGEFGFMIMND
                     RYQTFSQLGTVVNMAKRYSAIVNNGKDYTGKAVLALTEQGDPLALKERQVFLQSLAIG
                     IFNIQHAFDPQLILIGGGVSQADFLLSAIEAELDKLYQAVEISDLRPQLAICHFKNEA
                     NLLGAAIDFYQEHKEA"
     gene            11519..12469
                     /locus_tag="VGT87_000470"
     CDS             11519..12469
                     /locus_tag="VGT87_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004219264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEQ3813355.1"
                     /translation="MTRQTNKKTSFWKNVIKYRALLLMVLPGFIWFIFFFYIPVLANV
                     VAFKDFHYSAGGFMESLKESPWVGLANFKYLFASKDAWLITRNTIAYNVIFLLFNVFF
                     AIAFAIIMSELRNKRTVKVYHTMSLLPYFLSWVVIEYFVSAFLNTDKGFINQLLTGSG
                     ADPIKWYSNPTWWPLILLFMSVWKGLGYNSIIYYASVKGISDTYYEAAMVDGASKWQQ
                     IRNITIPQLLPMMSILLIINIGNIFKSDFGLFYVIPKNSGPLYDVTSVLDTYVYNALT
                     ATGDIGMASAASLYQSVVGTSILLVTNAIVRRMDPDAALF"
     gene            12479..13405
                     /locus_tag="VGT87_000471"
     CDS             12479..13405
                     /locus_tag="VGT87_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEQ3813356.1"
                     /translation="MEKKKKVEKVNVRTFDRKTNAIFNILIGLFAISCIIPFIFVIII
                     SFTDESYLINHGYSFFPDVWSTKAYQYIFQGAMSHRIMRSFGISVFITVVGTFINTTM
                     TSTYAYAISRPYFPYRRFFTVYALITMLFAPGMVANYLVVSNLLHLKDTVWALILPMA
                     LGPFGILVMRTFFKKTVPDSIIESARMDGASEWMIFMKIVLPLAVPGIATISLFSALT
                     YWNDWFNALLYVQSENLYPMQYLLMKIQSNLQALAQNAGMSAQMADSLASLPKESVRM
                     AIVVIATLPIALTYPFFQKYFVGGLTIGGVKE"
     gene            13486..14931
                     /locus_tag="VGT87_000472"
     CDS             13486..14931
                     /locus_tag="VGT87_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEQ3813357.1"
                     /translation="MKKWQKIVCVTGTVLSASSLAACESKSASKDSDVKLLMYQVGDK
                     PDNFDELMTIANKRIKEKTGATVDLQYIGWGDWDDKMSTIIASGENYDIAFANNYVVN
                     AQKGAFADLTTLMPKYAKKTYKNLDPAYIKGNTIDGKLYAFPVDANVYAQQMLSFNKE
                     LVDKYGLDISNIKSYADAENVLKQFHEKEPNTAAFAIGQVFSMSGDYDYPLTKTQPFA
                     VKIDEGKPTIINQYEDESFKNNLRLMHKWYKEGLIPTDAATNTEGYPLEGNTWFMREE
                     TQGPMDYGDTILTNAAGKDIVSRPLTKPLKTTSQAQMANFVVSSVSKNKEKAVEVLSL
                     LNSDPELLNGLVYGVEGKAWEKIGDKKIKLLDGYQPKMHMGAWNTGNNKILYTQESVT
                     DDMIAKRDQSIKDAKESPILGFTVDTKVIKTELSNISNVMNRYKASINTGTVDPDEAL
                     PKLLADLKGAGWDKVQKEVQKQLDDFVAKDK"
     gene            15237..15842
                     /locus_tag="VGT87_000473"
     CDS             15237..15842
                     /locus_tag="VGT87_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEQ3813358.1"
                     /translation="MNGIEKTCFLIWKMIQLTLIFHLLSLAGLIVFGVGPAWQTIVTL
                     FLKTPQEENHYSLKRAVQLWKSYFKEANLRFGLFALTFLFLTFNLHWAVQFPSLFWFT
                     VSFLIVIAMVWLTITYLYMVFYAVSYEINLWNNMKLAFISVFLSFKSFLLMLSVLLGI
                     TLVTWQYKGLYLFLTFGALVFCLDFVTKANRRLVDGVIDEC"
     gene            15832..17556
                     /locus_tag="VGT87_000474"
     CDS             15832..17556
                     /locus_tag="VGT87_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ3813359.1"
                     /translation="MSVKTLGRYVTNHFKQGFLINRLMKLYSLLIVSFFTLAAIGLSS
                     YSISSTYKRVDAEAQMRLEETLGRLQLQNDITLRVLDQLVGRSEDYHNLYQYMTLTPN
                     QYFSNVFEDWEKGKDTVLFSEEVRRLFDLYPDVTSMTLLLEDSSAYLYADRVIKTGRL
                     LYSKPEKVTGNVLVRSIRNPESGDVTGRLYLTFDKPPTLVETQQDHYLATFAFDYYGR
                     KLFHQGGRRFPHLEAEVKKAIKADRAADLSNLSKAYRMQYNRSGDLLAYVAVRKSYLL
                     AEAVRTVFVYGLVSLLLAWLLLQLLFRVFRNYIQQVSEITDTVEMVAAGDLSLTIDNS
                     HMELELYHISEAINQMLASIKAYIDEVYVLEVEQRDAQMRALQSQINPHFLYNTLEYI
                     RMYALSCQQEELADVIYAFASLLRNNISQDKMTTLKEELAFCEKYIYLYQMRYPDSFA
                     YHVKIDESIADLAIPKFVIQPLVENYFVHGIDYSRNDNALSIKALDETDHLLIQVLDN
                     GRGISQERLADMEKRLQEHQTTGNSSIGLQNVYLRLFHHFRDRVSWSMAKEPNGGFII
                     QIRIRKDA"
     gene            17556..19040
                     /locus_tag="VGT87_000475"
     CDS             17556..19040
                     /locus_tag="VGT87_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ3813360.1"
                     /translation="MYKVLLVDDEYMILQGLTMIIDWQALGFEVVQTARSGKEALAYL
                     TQYPVDVMISDVTMPGMTGLDLIEAAKTYHPQLQTLILSGYQEFSYVQKAMELETKGY
                     LLKPVDKAELQAKMKQFKDCLDAQQAESIRQEAYHDSLLTLWLTDELSEKEFQQLSQG
                     LPAAALTGFTVLYLDCQEWQAAIDAYFEREGQPFYLKKEEDKKVYLAVLLGKTDQAKA
                     FTYDLQKRFSRNMHQLILGETVDDWENVYESYNQVLKSLFYNCEVSPIRAKDKSKIEL
                     PESRLQFFAFNKALMIGDEPTILSKLEAIFDEMKTLNFSPEDVKHVSFLLFSDIYRQF
                     PILDKMTYLSMVKTIHDSQSIDRILRELKKVLDVTNQNNSPEKRYSDLVSETIDCIRK
                     EYHQELTLKAIADRLHVNGVYLGQCFKNETERSFTQYLNHVRIQKAQQLLLYTNQSIN
                     EIAYETGYNTNHYFIKMFKKLNGLSPKEFRDRYKDNYQAIKGDQ"
     gene            18970..22476
                     /locus_tag="VGT87_000476"
     CDS             18970..22476
                     /locus_tag="VGT87_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HEQ3813361.1"
                     /translation="MVYRQKSSEIVTRTITKPSRAISNVKQEIDMTPDSKEQTVTGYQ
                     YHYIDQEGRKQPFNQGWRFLMADVACAQDPSFDDSNWQVIHLPHDFSLKQPYTRNAEA
                     ESAYKLGGVGWYRHYLVLDEVLAGCHVAITFEGSYMETEIYVNGQFIGKHLNGYQEFT
                     YDISDVVTFGAENLLAVRVENKVPSSRWYSGSGLYREVSLSVLPQLHFVADQVAMTLA
                     DTAVQEKGQQKVDLRFSLNQSIQTCHYQLSLCLWEQSHCSKDKKLLYQETEVPLADLA
                     FQRQYGLTLSLEELQLWSPDNPHLYDLELTLYYQGQVIDCFCLETGFRQLTFMANQGL
                     FVNGRAVKLKGVCLHHDQGGLGACAYEDALARQLVLLKDMGANTIRSTHNPSSPKLRQ
                     LANRLGFFVIEEAFDTWTYAKNGNVNDFSNYFHQTIGTENANYLQRVRSPETSWAQYS
                     IEAMVWSAKNDPSVLMWSIGNELMEGFSADVSHYPELTRQMCQWIAAIDTSRPITFGD
                     NKLKEADFCWHEEVSQMATLLSQLDHPQGLIGLNYASGKDYDKLHEEHSDWLLYGSET
                     ASAITSRAYYKETKKVLDSGYHLTSYDHAKVDWGAFASQAWYDTITRDFVAGECVWTG
                     FDYLGEPTPWNKIDSGVVGLWPSPKNAYFGILDTAGFPKDSYYFYQSQWAQGQTTLHL
                     LPVWQKDQLCFDEQGLVEVVVYSNAASVQLMFEDEQGNVTDYGRKAFHTYSTPTGHTY
                     QLYQGADAAKNPHENLYLTWRVPYQKGLLRAVAYDISGKSIPKTSGRSQVRTYGSVAK
                     LSWKAFEAPIDAPWELLYLDLSLLDSRGELVSHAQDWLKVQVEGPAHLLALDNGNPTD
                     HTPYQEPLRQAYGGKLLAILALTGEAGHIKVTAKVSQQLSSVFQTQVACRAPKEHLVT
                     LPLEKYQFKKSRLTSQQEIGQSCLKTTQFLSVLENKDTQQKAGKVSTPHILITSYQHY
                     LSLPLQVCSQAFDFKKGDDIFLPQWLQVKDAQGSFVKKAFPMTWYLKEKIFAKPILVT
                     GYVSCFGQSIPVEARLNLAQASKRRHQDLSLVAKQSLATSQKRLTYCYQYDTVQAIGA
                     ISLQNHTDTSLSVSVAISYGSQSSIKQETLMLADSHLSQLEEVIFFDDILTVLYLVVT
                     LEVIEGPDLTEDSIQIGLWDLA"
     gene            22614..23492
                     /locus_tag="VGT87_000477"
     CDS             22614..23492
                     /locus_tag="VGT87_000477"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020905350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEQ3813362.1"
                     /translation="MSERLSGHTLLVSLLATPIRHSLSPKMHNEAYAKLGLDYAYLAF
                     EVGTEQLADAVQGIRALGIRGSNVSMPNKEAILPLLDDLSPAAELVGAVNTVVNKDGK
                     GHLVGHITDGIGALRALADEGVSVKNKIITLAGVGGAGKAIAVQLAFDGAKEIRLFNR
                     QATRLSSVQKLVTKLNQLTRTKVTLQDLEDQTAFKEAIRESHLFIDATSVGMKPLENL
                     SLITDPELIRPDLVVFDIVYSPAETKLLAFARQHGAQKVINGLGMVLYQGAEAFKLIT
                     GQDMPVDAIKPLLGDE"
     gene            23744..24499
                     /locus_tag="VGT87_000478"
     CDS             23744..24499
                     /locus_tag="VGT87_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEQ3813363.1"
                     /translation="MVTKEVGHDFLARLGKKRLRPGGVEATNWLIEQGQFSQDCHVLE
                     VACNRCTTAIELAKTFGCQIDAVDLNPKVVKEAQERVTVEGLTRHITVSQANALSLPF
                     PDNHFDIVINEAMLTMLNAKAKEKALNEYWRVLKPGGRLLTHDVAYEDPKTRQVLEEL
                     RQTIHVNVAPLSLTGWQALYQEHGFSQVTYHHGKMSLMTPRGMIKDEGLMNTLNIMRR
                     GLKKENRQQFLKMRRFFTRTGKNLCYIAVKSVK"
     gene            24552..24890
                     /locus_tag="VGT87_000479"
     CDS             24552..24890
                     /locus_tag="VGT87_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002265614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEQ3813364.1"
                     /translation="MSYINNIEHAKVLDLTQEVMIEQDQMLSRTLVQRQDLGITVFSL
                     DKGQEIGRHSSPGDAMVTILSGLAEITIDQETYRVAEGQTIVMPAAIPHALYAVEAFQ
                     MLLVVVKPEA"
     gene            24892..25197
                     /locus_tag="VGT87_000480"
     CDS             24892..25197
                     /locus_tag="VGT87_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEQ3813365.1"
                     /translation="MIENLSQLVQYHDHQVASRSLSRLLGLSQSIVLYAMAQGESISQ
                     ETSPRDKVILVLEGQLIFDLEDQKQVLTQESLIAIPAQKAHHLEAKTDCKLLQVLLD"
     gene            complement(25633..26706)
                     /locus_tag="VGT87_000481"
     CDS             complement(25633..26706)
                     /locus_tag="VGT87_000481"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEQ3813366.1"
                     /translation="MPQTLHVHSRVKDYDILFTDHVLKTLADCLGERKQRKLLFITDQ
                     TVYHLYQTLFEEFAQQYNAFVHVCPPGGQSKSLKRVSAIYDQLIAENFSKKDMIVTIG
                     GGVVGDLGGFVAATYYRGIPYIQIPTTLLSQVDSSIGGKVGVHFKSLTNMIGSIYPPE
                     AVIISTTFLETLPQREFSCGISEMLKIGFIHDRPLFQQLRDFQKETDKQGLERLIYQS
                     ISNKKRIVEQDEFENGLRMSLNFGHTLGHAIESLCHHDFYHHGEAIAIGMVVDAKLAV
                     SKGLLPKEDLDSLLQVFERYQLPTTLERADVSATSLFDVFKTDKKNSEQHIIFILPTE
                     TGFTTLAINKDDHQFVEKLDSLL"
     gene            complement(26762..27514)
                     /locus_tag="VGT87_000482"
     CDS             complement(26762..27514)
                     /locus_tag="VGT87_000482"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEQ3813367.1"
                     /translation="MIDIMSDFQNKTCSKNNFIVGPCSIESYDHIRLAASSAKKLGYN
                     YFRGGAYKPRTSAASFQGLGLQGIRYLHEVCQEFGLLSVSEIMSERQLEEAYDYLDVI
                     QVGARNMQNFEFLKILSHIDKPILFKRGLMATIEEYLGALSYLQDTGKSNIILCERGV
                     RGYDVETRNMLDIMAVPIIQQKTDLPIIVDVSHSTGRRDLLLPAAKIAKAVGANGIMM
                     EVHPDPDHALSDAAQQIDYKQLEQLGQELWQD"
     gene            complement(27755..27825)
                     /locus_tag="VGT87_000483"
     tRNA            complement(27755..27825)
                     /locus_tag="VGT87_000483"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27791..27793),aa:Thr,seq:ggt)
     gene            27992..28246
                     /locus_tag="VGT87_000484"
     CDS             27992..28246
                     /locus_tag="VGT87_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEQ3813368.1"
                     /translation="MSYEKEFLKDFEDWVKTQIQVNQLAMATSQEVAQEDGDERAKDA
                     FIRYESKLDAYEFLLGKFDNYKNGKAFHDIPDELFGARHY"
     gene            28267..28662
                     /locus_tag="VGT87_000485"
     CDS             28267..28662
                     /locus_tag="VGT87_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEQ3813369.1"
                     /translation="MAKQKNRKKALKLEMKRNGLLKKAGQVFDKAVESVETAVDKTIS
                     AGKNLVEKGSQTVGNLTASKERLTLDEFAGQPVVAGIRSDLVETLYAEGIHSAQAFKE
                     WTEKDLLALKGIGPATVKKLVENGASFKK"
     gene            28947..29162
                     /locus_tag="VGT87_000486"
                     /pseudo
     CDS             28947..29162
                     /locus_tag="VGT87_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044523.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         8536 a         5563 c         6355 g         8708 t
ORIGIN      
        1 cttcaatttt taggatttat atttttagat attattgaaa ttatgttaac gttaaagctt
       61 tttagctttg taagtgcaat tccgcttcgc ctgaaaaata ttttttattt atcacttagt
      121 atggttttgt ttcaagtagt tttttgggct ttctttccag accatttcat ccttgatgtt
      181 gtgatgttgg ctcagtttct tttctttgcg cttatagccc tatactatgg taaatcaata
      241 aaagctaagt ttttaatgtt ttatgccttt tttccactag tgtctattag tttggttaag
      301 cgatttattg tattttttgt tatgccatta tttggtatgc cttattcagt tgttaaacat
      361 aatacactac tgatttacag cattacttgc tttagtattt ttttgattta tcgctgcatt
      421 caagtttttc atttcgattt ttcaacctgg cgccaatatt ttcaatcaca tagagctagt
      481 aaactattag tgtttactaa ctcttcgatg gctctatatt acttgtgtgt ccaagggata
      541 gatgtgatgt cgccttcttt atcaggactt gctacaacga ctgctcgttc aatcatagtg
      601 ctcttttatt tcattttgtt tcttacttta ttaattcatt tagagcgtta tgtaaaacaa
      661 aactctattg aggcaattgt gcaacaaaaa gaatatcgtg agctgattaa ttatagtcag
      721 caccttggat tgctgtatca agatattcaa gagcttaggc agctattaac taccgtgtcc
      781 agtcgtctta agattggaat cgaacaaaat gatatctcta ttgtgagact tacttatgaa
      841 ggtatcttga atgctgaaaa gaataatgct aaagatgaca gacttgattt aacttgttta
      901 gataaattac aagttgaagc aatcagacat attgttttag ctaaattaat tgaggcaaaa
      961 aataagaagc ttaaggttga ggtatcgatc cctaattgta ttgcaacgtt ttttctagaa
     1021 gtagtagatc tcactaagct attgtcattt ttgttagata atgctataga aatgagtttg
     1081 gagacaaagc aaccttgctt atcaatagca tttctggatc aaaaccataa acttgtcata
     1141 gtcattcaaa gcagtactaa acaaggacaa gatgatagtc aaagcgtgtt tgctataccg
     1201 gctttgaaaa aaagagatga ctggcaattt gacttaagga atgtcacgac cattttaaat
     1261 cgttatgact atctcacaat tagctcgcag attcatgatg gcattttaac ccagttaata
     1321 gaaatagcta agcctgactg atgttaaagg tatgaataat cactcaaaat caacttaaga
     1381 gtctgagaca atagtcccag actctttttg aatatgttaa tctattacta atgataatag
     1441 tttattgttt cttccaagct agagttaaaa tttccgcttt gccaagttgg ctgggtgtgc
     1501 tttgcttgct gtctttttct aacaaattag tgaccttggc attgtagttt ggcaggctta
     1561 ggctaaagtc acaagtttta tcgtttgaga gattatagct gcgcgtgata agggcattgt
     1621 ctgctaagcg acgtttgaaa gccgttagag cgacttgatc gtctgcccca gtgacatagc
     1681 tatgctctgc tcttaagctt cccttatgat ggctggtttg ttcgactagg ataggaactt
     1741 ggccttcttg tgcccgccaa tagctggcaa attgtgtttg cttagtgatg ctttcaaaac
     1801 tataagatag gctgtgtttg ccgaggcatt gggcttcagg agtagggaaa tacccccagt
     1861 cgcccatttc accaacagaa cgtaagagag tgatggcaat ggtgttggta tctggtaaga
     1921 tctcatattc gttgaggccg tagttaccaa tagtgacccc cttttcacca tcaaagagac
     1981 tcacaaagca ttcttggtgc tgtgggttac ttggattctt ccaaaaggca gcatttggat
     2041 gatttggacg tttgacagtt tcaaaaatac tgtcagctag gtgatggtct gtcttaaggt
     2101 gcgttgggaa taagacgcgc aagcgatgat tagtcatttg gttatcaaaa cttgtggtga
     2161 attggaggcg aggattgttt ttctccatgc ggataagggt tgttaaggtc agttcagctt
     2221 tttcttgtga acgtcctgat tggcgttccg tgatgtcaat gacagcctcc atttcagcct
     2281 gtaagagctt atctgcggag acaggaatgg caaaggtttg ctggatttca agttcagcaa
     2341 cttgagcggt gttgctaatg atgttaagct tggtggtccc ttggtctgca tagaaaggtt
     2401 ggtcatgatt tggttggcgg gaaatgtatt catttccaat atcgccacaa tcttcaaacc
     2461 gcaacaggtc ttgatagatt agtccagatt gtttatcggt gatggttaga cgaccatcgg
     2521 tttgtatcat aacttttaga aactcatttt caaggcactg attactatcg tcgtagagtg
     2581 gaaccgttgc tgaaggagct gtctcgtttc ctagttgtaa tgctaaggct ttccaagaca
     2641 tggcagggag agttattggt agccgtaatc tcactttgat ggcgaaataa ggttctcgga
     2701 aggttctctt ggacaaatcg tagtcaaagg cgatgcttgt gtccaaaatg tctgcttctg
     2761 agatagtgtt tcctgataca tcaatgactt ggaaagattg ggagagttct gccaaatact
     2821 cttgagcttc ttggtaaacc tctttaggaa agcgttggcc aaaataatat tttttccagg
     2881 tcagggtgag ctcagtaaca cttgttttgg aatggccact ggtattaaat aagaggaaag
     2941 gttggctatc tgttggaaag tcactggtat caatggcgtc agcaatttgc ttagcagctt
     3001 cttttgctaa ataatgtccg acttcatagg ctttttcaaa gcgcgtcatc atttcccgat
     3061 gaacgctatc aacactacaa ccacagatag aatcatgagg gtgattttgc atgagggttt
     3121 tccaagcgta acgcagttgg tcgtgagggt aggtacctgt tacatcataa gccattgttg
     3181 ctaagggttc ggtgatgttt tcgagttggc gagagactct ggtattagcc tgtttgaggt
     3241 aaatgcgagc agaagctgtg ttagttaggg tataccagcc atcggtttct tgactggtaa
     3301 tctctccttg gacggttgaa agattctctg gtaaatcatc tgcgagatca gccaagtaat
     3361 cttcaaagca ggaatgcaca aattcgtagt caggatagag ttggttggct aaggcgattg
     3421 ccttggtgac atcaagttgt acgggttgat gatcacaccc gttcatcatc agaaggtgct
     3481 tggttgaggc aaagcgttca gcgtcagcaa gttttttatc ccaaaaaaga cgcgcctcag
     3541 cttctgttgt cgggatctca ttgccattgc tgtaccagtt ggcgaaaagg agtccaagaa
     3601 tatggctgtc atctggtcct tgccaactga tttcagagaa ttgggagctg tatttttcac
     3661 gggtatccac ctgattgtta aatccagttg gacgaatgcc acgaccaaag gcagcagctt
     3721 gtaggccggc ttttaacatc agctgtggtg tttgtcccat atttccaaag gtatcaggaa
     3781 aataaccaag tggcacacta gcgccccatc tgtcacaatc ttccttacca atcagtatat
     3841 tgcgcacatt ggactcgctg ctggtcaaaa aatcgtcctg taagatatag aaaggtccga
     3901 tacggagttt tcccgaagca atggcttgtc taatctcagg ttctcgttcg gggcgtactt
     3961 ttaaataatc atctaggata atggtttgcc catccaaatg aaaactatga aaatcaggat
     4021 ccgtttgaaa aacttctaac aggtcatcta ttaagttaat cagacgcatg tggtgttgtt
     4081 cgtaagccat gtaccactcg cgatcccagt gactgtgtga aataatatgt acttttttag
     4141 ttgccatagt ggcctcactt tctaaggagc aatactaaag cccagatagt caagaagtag
     4201 ttcacagaac atcatgttag cccaagagaa ccactcacga gtgtattgat aaggatcatt
     4261 gacatcgaaa ccctcgtgca ttaagtgggt accagcatct gttgccacaa gcaaatcaag
     4321 catctctttt ttactgtctt ggtcaagagc ggtcaatcct tgtaagacaa gggcgatatg
     4381 ccagatataa ttttctggtg tatgggaaga accgatgccc gctgctgcat ttccctgata
     4441 ataataaggg ttttcttggc taagaatggt gcggcgcgtt gccaaataga tagggtcatc
     4501 tttagcgcaa aaaccaaggt aaggtgcagc aaggaggctt ggaatattag catcatccat
     4561 gatggaaaac tgtcccagac catccacttc ataagcgtaa atggtttctc ccttgctgtt
     4621 ttgaacaagg gcatggtctg caatacccga ttgaatagct tctttcagct gcttggcacg
     4681 agtagctact ggttcattgt gaaaaaggtt gttatataaa tcttctagat aggaaagcac
     4741 aaccactgca aacatatttg aaggaatgag gtaaccatat tgacaagcat catcacttgg
     4801 tctaaagcct gaccaggtca tccctgttgg tgcgacagga cttccttttc cagcatgtgt
     4861 tagggtatct tctttgcgcc aagtatcgcg ttcaaacaag taaggagact gggcgtggtc
     4921 ttgctcaact gtccataggt caagaatgag ttctacccca cgatgaaaat cgtcattaaa
     4981 ctgatcggtg cttcctgttt cacgatagag caagtaagct agctgaatag ggtagcagag
     5041 ggaatcaatc tcatatttgc gttcccaaat ccaagggttc atttgggtac gatctgtttg
     5101 gtgtcctttt ccgttagctt cttcgttgaa ggcattggca tagggatcga tacaaatgta
     5161 gcggaattgg cgtttgacca aaccggcaat aatctttcta atttcttcat cttccttggc
     5221 gaggaagagg taaggtttca tttgcgcagt ggaatcacgt aaccacatgg caggaatatc
     5281 tcctgtcaag agaaagctag tgccatcttc gtaggttttt agggttgtct ctaaggtatt
     5341 ggtaaagctg gttttgaaat taagagccca atcagggtgt tggtctccac aaaggctttc
     5401 caccttggcc ataaattggc tgacgctagt tgggattggt cgtgtcatat catatcctcc
     5461 aaaaagatat atcatttatc ttcggtttca ttataaatga taagttaaac tttgtctata
     5521 agtttttgaa aaggtttaca actctttttt gacatgctat actgataacg ggagtaaaga
     5581 atgacaaaat tattatacaa aaagatatat catttcatta aagagcagat agcatcaggt
     5641 cgtttgcaaa ttggtgaccg cctacctact gaaaaagaat tgtcagaaca gttctcggtt
     5701 agccgtatta cttctaagcg agcgcttgta gaactggagc aagaaggctt gattacgaga
     5761 agtcgtggga aaggaagctt cgtagctgaa aagcaagtga cgtcccctga agcgaataag
     5821 gatttattgt tgattcttcc ttttgcttct gattatgaat tgggagatta tgctaaggga
     5881 attatgacct ccattgctga aacaggttac cgtctcatgg ttcaattggc cagtacagta
     5941 aggttagaca ccctgtcaga ctatgctggg attatttatt atcctgaaga tgtcaatcac
     6001 agtattgatt tcctgtttta ctgcgaccat catcacattc ctctagtctt gttggataaa
     6061 tctctagatc tttttcagtt tccgtctgtt gtagcggata ataaaggcgg tgcctgtcaa
     6121 ttgactcagc acttgattga gcaaggctgt gaccagatta gttttgtggc aacaggctct
     6181 tttggagaag tatcatccgt tagagaccgt tatttgggtt acttagcggc aatggcagaa
     6241 tcatctctaa aatcccatta ttttcctaag acagagactg aaacaacaga tgcttatttg
     6301 gaccgtttgg tgaaaacctt atcggaaacg acagccacta aaagggggct tgtcgtggaa
     6361 aacgattggt tggctattca gctgattcaa aaaatcacac aggtaggctt atctattcct
     6421 gatcaagtcg ctattgtagg ctttgataat agtcaagcct cccgtttgtt gagtcccaaa
     6481 ttaagcacag cagctcaaga tttttaccaa atggggcaag aagcagctaa agtcttgttg
     6541 cagattattc atgagccaga ccaagcagtg gtgtcttgcc agttacctgt tcaagtattt
     6601 atcagagaaa gtagtcacta aaaaagacgt tactagcatg agctggttag ctcaggtaaa
     6661 ggagaccaga aatgaccatt tatcacgcct taaaggatta tcaagaagtc atgacaagag
     6721 gagactatct tgtctttgac acgccattga cttgccgatt tattggccgt tttttccgct
     6781 ttgaaaatca aaaagccctg ctggcagaat tggcaacgtc aaagtatttt cagtggattg
     6841 aagaaggcca agcagaggtg acgatgaaac acttttttaa ccgtcagctg gctaaggatg
     6901 cctttaccct gaaggtttca gaagataagg aaatcattat tgaaagccaa aaccttagag
     6961 gttttcgtta cgcccaagaa gctttgctta aagtcatgac cttcaaaggg gataaacttt
     7021 atttgcccat tgtttctgtc aagcacagcc cgtcatttcc tatgcggggg ataattgaag
     7081 gcttttatgg aacaccgtgg acacgagagg aacgaatcga ttgcctacgt tttattggaa
     7141 ataaaaggat gaatacttac atgtatgctc cgaaagatga tgattaccag cgaaaactgt
     7201 ggcgcgatct ttatcctgag gattgggtga cttatttcaa agagctactt gtagtagcca
     7261 aggaagaagg gattgatttt tggtatatga ttagtccggg acttgatttt gattatacca
     7321 aggaagctga ctatcagttg ctctatcaaa aattgcaaca acttctagcg ttaggtgttt
     7381 gtcattttgg gcttttgctg gatgatattg actaccaaat tgtagatgct gttgagagac
     7441 gattcaaaaa gactgcctat gcccaggccc acttggcaac tgaggttcat cactttttaa
     7501 accaacaaca tgcggcgcca gaattggtga tctgtccgac agaatacgac aaccaccatg
     7561 attctatcta tttgcaagag ctgtcagaaa ggattcctaa agaagtcgcc tttttctgga
     7621 caggcccaag cacgctagcg agtcaaatct cgcaagcaga tatcgaaacc atggcagcag
     7681 tatatcagcg gcctatcatt atttgggaca atattcctgt taatgattat caaaaagatc
     7741 cagaacgtct ctttttaacc ccttttgcta atcgctcacc atttttatgc caatcagact
     7801 atcaggtcaa ggggattgtt tctaacccta tgattagttg ggaattatct aagctgacct
     7861 tgacagacat gagccattat ctgtgggatg ctaaccgtta tcagcctagt aactcttggc
     7921 tagaaacctt aacagactat acaggagatg ctgaattagc cttagcttta caggcatttg
     7981 cttggcataa tggcaatcgt catctgcaca gggaccttcc ttttgaagtg gaagaagctc
     8041 tcctggctaa ggatgtatca acgttatcag cttgggtaga tgaattggta gaacgtgcca
     8101 atacgttgag aaagctagat aagccagcgt ttcaacaagc tattgttcct tggtttgaac
     8161 gtgtggccaa agaccaagat ttttggcagg ccattttaga gcaggggcct catttggaaa
     8221 cactctatgc tgacttacaa gaagataaac accgtattgg aagtgacatt ccaagtcgct
     8281 actatcgcat ctactatcaa cagcaagata aactaacagc taatcaaggt caagttaccc
     8341 aagcaagacc tgaagattat gcttagtcat acgagctaga aaggaatgat atgttagcat
     8401 ttccaaaaga gttttggtgg ggaggagcca cttcaggccc tcaaagtgag gggcgatttg
     8461 ctaaacagca ccgcaatctc tttgattatt ggtatgaaga ggagcctgac ttgttttacg
     8521 attacgtggg acctgatacg gcttcggatg cctatcatca gattgaatct gatttggcct
     8581 tgttagcttc actaggtcac aattcctacc gcacctccat tcagtggaca cgtttgattg
     8641 atgattttga gcaagcgacg attaatcctg atggccttgc ttattataat cgagtgattg
     8701 atgcttgctt ggccaatggt attcgtcctg ttattaattt gcatcatttt gacctgccaa
     8761 ttgccctcta tcaagtctat ggtggctggg aatcaaagca tgtcgtggac ttatttgtcg
     8821 cctttagcaa ggtttgtttt gagcagtttg gtgatcgggt taaggattgg tttgtccaca
     8881 atgaacctat ggtcgttgtg gaaggctctt atctaatgca atttcattac cctgctatcg
     8941 tggatgggaa aaaagcagtc caggtcgcct ataatctagc cctagccaca gccaaggtta
     9001 ttcaagctta caggcgaggt ccagcagaac ttagtgatgg gcgtattgga accattctca
     9061 atctgacgcc agcctaccca gctagtcaat cagaagcgga tacggctgct gcgcattttg
     9121 cagaactttg gaacaatgac ttgtttatgg aagctgctgt tcatgggaaa ttcccagaag
     9181 aattggttgc ggtattgaaa aaagatgggg ttctgtggca atcaacgcca gaagaaatgg
     9241 ctcttattgc tgagaataga gtggattatt tgggattgaa tttttaccat cctaagcgcg
     9301 tgaaggctcc tgatgccatt ccagtgatta gcccatcttg gagtcctgaa tggtattatg
     9361 acccctacct gatgcctggc cgccgcatga acgtggataa ggggtgggag atctatcctg
     9421 aggctgtcta tgatattgcc atcaagatgc gagaccatta cgacaatatt ccttggttct
     9481 tatctgaaaa tggtgtaggg atttcaggag aagaccgtta ccgtgatgaa actggtcaga
     9541 ttcaagacga ttatcgcatc caatttttga aagaacattt aacctatctc cacaaaggaa
     9601 tagaagcagg ctcgaattgt tttggatacc atgtatggac gccaattgat ggctggtctt
     9661 ggctcaatgc ctataaaaac cgctatggcc ttgtggaaaa taatatccat acccaagtgc
     9721 gacgacctaa agcttcagct tattggttta aaaaagtagc tactcataac cgcttgattt
     9781 cattagaagt aatggaggag tttggaggct ctgcttctca cctcagtgat tgaacgtgtg
     9841 aacgtctcct ttgagacaag tgaataatga tgttaccaat gattaaacaa gggccttttt
     9901 cctcatgtta agaggatcga ggctcttgta tttctttgga aggtgttggt atttgccgaa
     9961 ctagatataa gcgttctgtc tggtttggca tcatctatcc atcttgggta atttggctct
    10021 tcaattcttt agagtatctt ggctaaggat gcttgttttt catcttggta ttttaggatt
    10081 ttctttcata tttttggacc agataaaagc ccctcatggt ttctagtgac tgtttgttat
    10141 gtcactgtcc acgataaggg gactatatga gagagttatt aagtgaggct ttgcttgttg
    10201 gcgataacta gcatagcttc taatagctcg agtattaact gatgaccaat gcctttgaca
    10261 gaagcgtcag caacaacgac atcaacaaag ggaccaggca tcaggctgcg ttcatcagca
    10321 gagagtcatt taggcagaaa gccagtatcg ttgtagctaa tcagcaatcc ttttaggatg
    10381 tctttgtaga tgtcagaggc gattagggac aataggggat aagagggatt aagcggtcca
    10441 tggttaggcc tccttgtgtt cttggtaaaa atcaatggca gctccaagaa gattggcttc
    10501 gtttttaaag tgacaaatgg ctaattgggg tctaaggtcg gagatttcga cggcttggta
    10561 gagtttgtct agttcagctt caatcgctga cagtagaaag tcagcttggg aaacaccacc
    10621 cccgattaaa atcagttgag gatcaaaggc gtgttgaatg ttaaaaatac cgatggctaa
    10681 actttggaga aagacctgcc tttctttgag tgctagggga tctccttgtt ctgtgagggc
    10741 aaggacagct ttgccagtat aatcttttcc gttattgacg attgcactat agcgcttggc
    10801 catattcaca acagtgccta attgactaaa ggtttggtag cggtcgttca tgatcatgaa
    10861 accaaattcg cccccaaata agtgggcacc atggtaaatc ttgccatcaa tgaccaagct
    10921 tcctccaact ccagtaccaa taacgagcat ggctaggcta ctagcgcctt ttcctgcccc
    10981 taaggtagct tcagctaagg cggcacaatt ggcatcattt tcgatagaaa tagggtaatg
    11041 tagccgttct tctaaggcct cttgtatttt aaaatggtga atgtagggaa tagcgctggc
    11101 gccttcgata accccctttt ctttattgac tgccccaggt gaactgatgg caatacctga
    11161 gaaatggtaa gcagagtaac gagcaacttc ttggtcgagt aattggtaaa acttttccag
    11221 actagaggga gtgggaaagc tgctttgctg gcttaattgg ccattgtgac agagggcaaa
    11281 tttaagggaa gtgcctccta tatcaagaca taacaagctc atgaggtgtt ctcctttatt
    11341 gatagtagaa tagacgatca tgtctcatct tagtatgaca gatttaagca acaatgaaaa
    11401 ggctttctaa gtgattctat actttgtgtt atcataccta taatttgttg ctttcaaata
    11461 atgaaagcgc ttgatatact gtaactataa cgtgattaac acaaagggag gttgggaaat
    11521 gacaagacaa accaacaaaa agacatcctt ttggaaaaat gtgattaaat accgggcctt
    11581 attattaatg gtattaccag gtttcatctg gttcatcttt ttcttttata ttccagtttt
    11641 ggccaatgtg gttgctttta aagattttca ttattcagca gggggcttta tggaaagttt
    11701 gaaggagagc ccttgggttg gtttggctaa cttcaaatac ctctttgctt ccaaagatgc
    11761 ttggttgatt accagaaata ccatcgctta caatgtgatt ttcttactct ttaatgtttt
    11821 ctttgcgatt gcttttgcca ttatcatgag tgagttacga aataagcgaa cggttaaggt
    11881 ctatcacacc atgtccttat tgccttattt cttatcatgg gtggttattg aatactttgt
    11941 ttctgccttt ttaaatacgg ataaaggttt cattaatcag ctcttgactg gaagtggagc
    12001 agatccaatc aagtggtatt ccaatcccac ttggtggccc ttgattttac tctttatgag
    12061 tgtctggaaa gggctaggct acaatagcat tatctactat gcttcagtca aagggatttc
    12121 tgatacttat tatgaagctg ctatggtgga tggtgcaagc aaatggcaac aaattaggaa
    12181 catcaccatt ccacaattat tgccaatgat gtctatccta ttgatcatta atattggtaa
    12241 tatctttaaa tctgatttcg gacttttcta cgttattccc aaaaattcag gcccacttta
    12301 tgacgtgacc agtgtgttag atacctatgt ctacaatgcc ttgactgcta caggagatat
    12361 tgggatggca tcggcagcta gcctttatca gtcagtagtt gggaccagca tcttgttagt
    12421 caccaatgcg attgttcgtc gcatggaccc tgatgcagcc ttgttttagg aggaaaacat
    12481 ggagaaaaag aaaaaagtcg aaaaggttaa tgttagaacc tttgatcgaa aaaccaatgc
    12541 tatctttaat attctgattg gtctgtttgc catctcttgt atcattcctt ttatcttcgt
    12601 gattattatt tccttcactg atgaaagtta cttgattaat catgggtata gtttttttcc
    12661 agatgtctgg tcaactaagg cttaccagta tatttttcaa ggagccatgt cccatagaat
    12721 catgaggtca tttgggatat ccgtgtttat tacagtggtg ggaaccttta ttaacacgac
    12781 catgacatca acctatgctt atgcgatttc aagaccttat ttcccataca gacgtttttt
    12841 tactgtttat gcacttatca ccatgctctt tgcaccaggg atggttgcta attacttggt
    12901 ggtcagcaat ctccttcatt tgaaggatac ggtttgggct ttgattttac caatggctct
    12961 gggcccattt ggcatcttgg tcatgagaac gttctttaaa aagacagttc cagatagcat
    13021 tattgagtcg gctcgtatgg atggggctag tgaatggatg atttttatga aaattgttct
    13081 ccctttggct gttccaggga ttgctaccat cagtttattt tctgccttaa cttattggaa
    13141 tgattggttt aacgccttgc tttatgtgca aagtgaaaac ctctacccaa tgcagtactt
    13201 actgatgaaa attcagagca acctacaggc cttggcacaa aatgctggca tgagtgcaca
    13261 aatggcagat agcttagcat cgctgccaaa agaatcagtt cgtatggcta tcgtggttat
    13321 tgcaaccttg ccgattgcct tgacttaccc attcttccaa aagtactttg tcggtggttt
    13381 gaccattggt ggggtgaagg aataggactt aaggaactgg tcacaagacc agattttatc
    13441 taatcattag taacaaaaga aaacattttt taggaggctt cacttatgaa aaagtggcaa
    13501 aaaatcgtgt gtgtgactgg aactgtgctt tcagcgtcta gtttagcagc ctgcgaaagc
    13561 aagtcagcat caaaggatag tgatgtcaaa ttattgatgt accaagttgg tgacaaacct
    13621 gataacttcg atgaattgat gacaattgct aacaagcgca tcaaagaaaa aacaggtgca
    13681 acggttgacc ttcaatacat cggttggggg gactgggatg ataaaatgag taccatcatt
    13741 gcctctggtg aaaactacga cattgctttt gccaataatt atgtggtcaa tgcacaaaaa
    13801 ggtgcttttg ctgatttgac aacgttaatg ccaaaatacg ctaagaaaac gtataaaaac
    13861 ttagacccag cctatattaa aggaaatact attgacggta aactctatgc cttcccagta
    13921 gatgccaacg tttatgccca acagatgctt tctttcaata aagaactagt ggacaaatat
    13981 ggccttgaca tttcaaacat taagtcctat gcagatgctg aaaatgtctt gaaacaattc
    14041 cacgaaaaag aaccaaatac agcagctttt gctattggtc aagtctttag tatgtcaggt
    14101 gactatgact acccattaac caaaacccaa ccctttgctg tgaaaattga tgaaggcaag
    14161 ccaaccatca ttaatcagta tgaagatgag tcctttaaaa acaatctccg cttgatgcac
    14221 aaatggtata aagaaggttt gattccaaca gatgcagcga ccaatacaga aggttatccc
    14281 cttgaaggaa acacttggtt tatgcgtgaa gaaacccaag gtcctatgga ctatggcgat
    14341 actatcttga ccaatgctgc aggaaaagac atcgtgtctc gtccattgac taaaccgcta
    14401 aaaaccacat cacaagcaca aatggcaaac tttgtggtat caagcgtatc taaaaacaaa
    14461 gaaaaagcag ttgaagtcct ttctcttctt aatagcgatc ccgaattgtt aaatgggctt
    14521 gtttatggtg tcgaaggcaa agcttgggaa aaaattggcg acaagaaaat caaactgctc
    14581 gatggctatc aaccaaaaat gcacatgggt gcttggaata ctggtaacaa taaaatcctt
    14641 tacactcaag aatctgttac tgacgacatg attgctaaac gtgaccaatc aatcaaggat
    14701 gctaaagaat ctccaatcct tggtttcact gttgacacaa aagttatcaa aacggaattg
    14761 tcaaatattt caaatgtcat gaaccgatat aaagcaagta tcaatactgg tacagtggat
    14821 cctgacgaag cccttccaaa acttcttgca gacctcaaag gtgctggttg ggacaaggtg
    14881 caaaaagaag ttcaaaaaca attggacgac tttgtagcta aagacaagta aggcattcat
    14941 tttcttgatt ataagttgag ggctgggaaa tctcagctct tttcttctga tgagtggaaa
    15001 aaacggatca tcaaataagt ggaagttata aagggagaaa aattaatgta aataatgtta
    15061 ctactcttag taaagcggtt tccaaaagcc tataaagtgt gctatattag aagagtctaa
    15121 actattaatc agagtaaaac taataccagc atcttactgg gggtgctaaa actagtctcg
    15181 tagctgctgg tccttgaaac cgctataagt gttaacaaga aaaagaggac tatcatatga
    15241 atggcattga aaaaacctgc tttttaattt ggaaaatgat ccaattgacc ctcatttttc
    15301 atctcttgtc cttagctgga ctgattgtgt ttggggtggg gccagcctgg caaaccattg
    15361 tcaccctatt tttaaaaaca cctcaagaag aaaaccacta ttctttgaaa agggctgttc
    15421 aactttggaa atcttatttc aaagaagcta atctaagatt tggtcttttt gccctgactt
    15481 tccttttttt aacctttaat ttgcattggg cagtacagtt tccatccttg ttttggttta
    15541 ccgtgtcttt cttgattgta atagccatgg tttggctaac cattacctat ctttacatgg
    15601 ttttttatgc agtgagctat gagattaacc tttggaataa tatgaaatta gcctttatca
    15661 gcgtcttttt aagtttcaaa tcctttttac tcatgctgag tgttttgctg ggaattacct
    15721 tagtgacttg gcaatacaaa ggcctttatc tctttttaac cttcggagct ctagtctttt
    15781 gcttggactt tgtcactaag gctaatcgtc gactagtaga tggagtgatt gatgagtgtt
    15841 aaaacattgg gaaggtatgt gactaaccac tttaagcagg gttttttgat taatcgactg
    15901 atgaaattat atagcctgct aattgtaagt tttttcactt tagcagctat tggcttgagc
    15961 agttattcta ttagtagtac ttataaaagg gtggatgctg aggcgcagat gcgactggag
    16021 gagactctag gtagattgca gttacaaaac gatattaccc tacgggtttt agaccagttg
    16081 gttggccgtt cagaagatta ccataacctc tatcaatata tgacgttaac cccaaaccaa
    16141 tatttttcaa acgtttttga agactgggaa aaaggaaaag acacggtcct attttctgaa
    16201 gaggtgaggc gtctttttga cttgtaccct gatgtgactt ctatgaccct actgctagag
    16261 gacagtagtg cttatctgta cgccgatagg gtcattaaga cgggtcgttt gctttatagc
    16321 aagcctgaaa aagtaaccgg aaatgtttta gttcgctcca ttcgaaatcc agaatcagga
    16381 gatgtgacag gtcgtcttta cctgaccttt gacaaaccac cgactttggt agagacacag
    16441 caggatcact atttagctac ttttgctttt gattactatg gccgcaaact cttccatcaa
    16501 ggaggtcgga gatttcctca tttagaggct gaggttaaaa aggctatcaa ggcagatcgg
    16561 gcagctgatc ttagtaatct cagcaaggct tatcggatgc agtacaatcg ctcgggagac
    16621 ctcttagctt atgtggctgt cagaaaatcc tatttattag cggaagctgt caggacagtt
    16681 tttgtgtatg gccttgtttc tctactttta gcttggctgt tattgcagtt gctctttagg
    16741 gttttccgaa attatatcca gcaggtttct gaaattacgg atactgttga aatggttgca
    16801 gcaggcgatt tgtctttaac catcgacaac agccacatgg aattggaact gtatcatatc
    16861 tcagaagcca ttaatcagat gttggccagc attaaagctt atattgacga agtttatgtg
    16921 ttagaggtag agcaacgaga tgcccaaatg agagctctgc agtctcaaat caaccctcat
    16981 tttttataca acacgttaga gtatattcgg atgtacgccc ttagttgtca acaagaagaa
    17041 ttagcagatg tcatttatgc ctttgcgagt ctgcttcgca acaatattag ccaagataag
    17101 atgaccacct taaaagaaga actggctttt tgtgaaaaat acatttatct ctaccaaatg
    17161 cggtatccag atagcttcgc ttatcatgta aagattgatg agagtattgc tgacttagcc
    17221 attcctaaat ttgtcattca acctctcgtt gaaaattatt ttgtacatgg gattgattat
    17281 agtcgcaatg acaatgcact aagcatcaag gcattagatg agacggatca tctcttgatt
    17341 caggtgcttg ataatggacg tggtattagt caagagcgct tagcagatat ggaaaaaagg
    17401 cttcaagagc accaaacaac aggcaatagc tctattggtt tgcaaaatgt ttaccttcgt
    17461 cttttccatc attttcgaga cagggtttct tggtccatgg ctaaggagcc aaatggtggc
    17521 tttattattc aaattaggat tagaaaggat gcttgatgta taaggtacta ttagttgacg
    17581 atgaatacat gattttacaa gggctcacca tgattattga ttggcaggca cttggatttg
    17641 aagtggttca aacagcaaga tcaggcaagg aagccttagc ctatttgact cagtacccag
    17701 tggatgtcat gatttcagat gttaccatgc ctggaatgac aggactggat ttaatagaag
    17761 cagctaaaac ctatcatcct cagctacaaa ccttgatttt atctggttat caagaattct
    17821 cctatgtgca aaaggctatg gaattagaaa ccaaggggta tttgctaaaa cctgtggata
    17881 aggcagaatt acaggctaaa atgaaacaat ttaaagactg cttggatgcc cagcaggcag
    17941 aatcaatccg gcaagaagcc tatcatgaca gccttttgac cctttggctt acagatgaat
    18001 tgagtgagaa agaatttcag cagctaagtc aaggcttgcc agcggctgct ttaactggtt
    18061 ttacagtgct atatttggac tgtcaagagt ggcaagcagc catcgatgct tattttgaac
    18121 gagaaggtca gcctttttac ctcaaaaaag aagaagataa gaaggtttat ctggctgtcc
    18181 tacttggtaa aactgatcaa gccaaggcat ttacctatga tctacaaaag cgattctcaa
    18241 gaaacatgca tcaacttatt ttgggagaaa cagttgatga ctgggaaaat gtttatgaaa
    18301 gctacaatca ggtgcttaag agcctctttt acaattgtga agtgtcacca attagagcaa
    18361 aagataagtc caaaattgaa ctaccagagt cccgtttgca gttctttgct tttaacaagg
    18421 cgttgatgat tggggacgaa cctacaatct tgtctaaatt agaagctatt tttgacgaga
    18481 tgaagacctt gaacttttca ccagaggatg tgaagcatgt ttcgttcttg ttgttttctg
    18541 acatttaccg tcaatttccc attttagata aaatgactta tctgtccatg gtaaaaacta
    18601 ttcatgacag ccaatccatt gatcgcattt tgagggaact aaaaaaagtg ttggatgtca
    18661 ccaatcagaa caattcccct gaaaaacgct attcagattt agttagtgaa accattgatt
    18721 gcattcgcaa ggaataccat caagaattaa cccttaaagc tatcgctgat aggttacatg
    18781 tcaatggggt ttatttaggg caatgcttta aaaatgaaac cgagcgtagt tttacccagt
    18841 acttaaatca tgttaggatt caaaaagcgc aacagttatt gctttacacc aaccaatcca
    18901 tcaatgaaat tgcttatgaa acaggttaca ataccaatca ttattttatc aagatgttta
    18961 aaaagttgaa tggtttatcg ccaaaagagt tcagagatcg ttacaaggac aattaccaag
    19021 ccatcaaggg cgatcagtaa cgttaaacag gagattgata tgacaccaga tagcaaggag
    19081 cagacagtga ctggctacca ataccactat attgatcaag aaggaagaaa gcagcccttt
    19141 aatcaaggct ggcgttttct tatggcagat gtggcgtgtg ctcaggaccc ttcttttgat
    19201 gattcgaact ggcaggtgat tcatttacca catgatttca gtcttaagca gccttacaca
    19261 agaaatgctg aagctgaaag tgcttataaa ttaggtggtg tcggttggta tcggcattat
    19321 ctcgtgttag atgaggtttt agctggttgt catgttgcca ttacttttga gggttcctat
    19381 atggaaacgg aaatttatgt taatgggcaa ttcattggca aacacttaaa tggctatcag
    19441 gaatttacct atgatatctc tgatgtagtg acttttggag ctgaaaatct tctggctgtc
    19501 cgggttgaaa acaaggttcc cagttctcgt tggtattcag gcagtggtct ttatcgtgaa
    19561 gtgagtttgt ctgttcttcc ccagcttcat tttgtggcag accaggtagc aatgaccttg
    19621 gctgatactg ctgtgcagga gaaagggcaa cagaaagttg atttacgctt ttcccttaat
    19681 cagtctattc agacttgcca ctaccaattg agcctctgtt tatgggaaca atcacattgc
    19741 tcaaaagaca aaaaactcct ttatcaagaa acagaagtgc ctctagctga tttggctttc
    19801 caaagacagt atggtcttac actgtctttg gaagaactcc aattgtggtc accagataat
    19861 cctcacctct acgatttaga actcactctt tactatcaag gtcaggtcat tgattgcttt
    19921 tgccttgaaa ctggttttcg tcagctgact tttatggcca atcaggggct ttttgtgaat
    19981 ggcagagctg tcaaattaaa gggagtttgt ttgcatcatg accaaggagg cctaggagct
    20041 tgtgcttacg aagatgccct agccagacaa ttagtcctgt tgaaagacat gggggctaat
    20101 accattcgta gcacccataa cccaagtagt cccaaactta ggcaactagc caatcgtctc
    20161 ggcttttttg tgattgaaga agcctttgac acctggacct atgccaaaaa tggaaacgtt
    20221 aatgactttt caaactattt ccatcagact atcggaactg aaaatgcaaa ctatcttcaa
    20281 agagtgagat cacctgaaac gagctgggcc cagtatagta ttgaggccat ggtctggtct
    20341 gccaagaatg acccgtcagt gttgatgtgg tcgattggta atgaattgat ggaaggtttt
    20401 tcagccgatg tcagtcatta cccagagctg acaagacaaa tgtgtcaatg gattgctgct
    20461 attgacacct cacgtcccat tacctttggg gacaataaat tgaaagaagc tgacttttgc
    20521 tggcatgagg aagtgtcgca aatggcaact cttcttagtc aattagacca tccccaggga
    20581 ctcattgggt taaactatgc tagtggaaaa gactatgata agcttcatga ggagcactct
    20641 gattggctgt tgtatggttc tgaaacagcc tcagccatta ccagcagggc ttattataaa
    20701 gagacaaaaa aagttcttga ctccggttat cacctaacct cttatgatca tgctaaggtt
    20761 gattggggtg ccttcgctag ccaggcttgg tatgatacca ttacccgtga ttttgttgct
    20821 ggcgaatgcg tttggacagg ctttgattat ttaggagaac ctacaccctg gaacaagatc
    20881 gacagcggtg ttgtaggcct ttggccttct cctaaaaatg cctactttgg cattttagac
    20941 acggcaggct ttccaaaaga tagctattat ttttaccaaa gtcagtgggc gcaggggcag
    21001 accaccttgc acctgttacc agtctggcag aaggaccagc tttgtttcga tgagcaggga
    21061 ttggtggagg ttgtggttta tagcaatgct gccagtgttc aactgatgtt tgaagatgag
    21121 cagggcaacg taacagatta tggaaggaag gcatttcata cttatagcac gccaacggga
    21181 cacacttatc agctttacca aggagctgac gctgctaaga atcctcacga gaatctttac
    21241 ctgacttggc gagtgcccta tcaaaaaggt ttgctaaggg cagtggccta cgacatttct
    21301 ggtaaaagca ttccaaaaac cagtggacgt tctcaggtta gaacctatgg ttcagtggca
    21361 aagctatctt ggaaagcctt tgaagcacct attgacgctc cttgggaatt gctttatctg
    21421 gatttatcac tcttggatag ccgaggtgaa ttggtcagcc atgctcaaga ttggctaaag
    21481 gttcaagtag aaggaccagc ccatttgttg gctcttgata atgggaatcc gactgaccac
    21541 accccttatc aggagcctct acgccaggca tatgggggaa aattattagc cattttagcc
    21601 ctcactggag aggcgggaca cattaaggtc acggcaaaag tctcgcagca acttagcagt
    21661 gtttttcaga cacaagtagc ctgccgtgcc cccaaagaac acttagtgac actaccttta
    21721 gagaaatatc agtttaaaaa gagccgactg acaagtcaac aagaaattgg tcaatcttgt
    21781 ctaaagacca cacaattcct ttctgttttg gagaacaaag acacccagca aaaagcaggt
    21841 aaggttagca cacctcacat tcttatcacc agttaccagc actatttatc tctgcccttg
    21901 caggtttgtt ctcaggcatt tgattttaaa aaaggcgacg atatcttctt gcctcagtgg
    21961 ctccaagtca aagatgccca aggttctttt gtgaaaaaag cttttccaat gacatggtac
    22021 ctaaaagaga aaatctttgc caagcctatt cttgtcacgg gctatgtttc ttgttttggg
    22081 caatcaatac cggttgaggc aaggcttaac ctagcacaag ctagcaaaag acggcaccag
    22141 gatctcagtt tagtcgccaa gcaatccctg gccacaagtc aaaaaaggct aacttattgc
    22201 taccaatatg atacggtgca agccatagga gccattagct tgcaaaatca cacagacaca
    22261 tctcttagtg tttctgtggc aatcagttat ggtagtcaga gctctattaa gcaagaaacc
    22321 ttgatgctag ctgatagcca cttgagccag ttagaagagg ttattttctt cgatgatatt
    22381 ttgactgttt tgtatctagt agtgaccctt gaagtcattg aaggacctga cctaactgaa
    22441 gacagtattc aaattggcct ttgggattta gcatgaagca taagtaaaag aagacttctt
    22501 tggcaccagg tctgatgaat tttgtaaaat aaggtataat aaaaaacaaa taggcgtcct
    22561 ttattacgtg aaaggcgcct ttacaaggtt gaaatggaaa gtgagatttc taaatgtcag
    22621 aacgtttatc aggccacacc ctgctggtct ctttgcttgc tactcctatt cgtcatagtt
    22681 tgtctcccaa aatgcacaat gaagcctatg ctaaactggg gctggattat gcctaccttg
    22741 cttttgaagt agggacagag caattagcag atgccgtgca agggattcgt gccttaggaa
    22801 ttcgtggctc caatgtgtct atgccaaata aagaagctat tttaccctta ttggatgact
    22861 tgtccccagc tgctgagttg gtcggtgcag tcaatacggt tgtcaataag gatggtaaag
    22921 gacatctcgt ggggcatatt acagacggca taggagcgct cagagccttg gctgacgaag
    22981 gagtgtcggt aaagaacaaa atcattaccc tagcaggagt tggtggggct gggaaagcta
    23041 tcgctgtcca gctcgctttt gacggcgcca aagagattcg tttgtttaac cgacaagcta
    23101 cacgtctaag tagcgttcaa aagcttgtga caaaactgaa ccagctaaca aggactaaag
    23161 taacccttca agacttggaa gatcagactg cctttaagga ggccatcagg gaatctcatc
    23221 tatttattga tgctacaagt gtaggaatga agcctttaga aaatctaagt ttgattaccg
    23281 accccgagct cattcgccca gacttggtgg tctttgacat cgtgtacagc ccagcagaaa
    23341 ccaagttact agcctttgcc cgtcaacatg gcgcccaaaa agtcattaat gggctaggga
    23401 tggtgcttta tcaaggagca gaagctttta aattaatcac tggtcaagac atgccagttg
    23461 acgctattaa acctctttta ggtgatgagt aactcaacct tttttaaaga actttttaag
    23521 taagcttaat tatgatatat taataagaga tggagcagtc atcatgggct gctaaaattc
    23581 tttttgcgag agctgttttt aagagtaaaa agcttatttg aagagcctta tccctaacgt
    23641 gatacaagtc atagttcctt tgaggtctgc cagataacct accgtttttt tagaaaagaa
    23701 gctcttataa aaggaaaagt tcaatgaata aaggagaata gcaatggtaa caaaggaagt
    23761 tgggcatgac tttttagcac gtctaggaaa aaaacgctta agacctggcg gtgttgaggc
    23821 gacaaactgg ttgattgaac aaggacaatt ctctcaggat tgtcacgttt tagaggttgc
    23881 ctgcaatcgc tgcacgacag caatagagtt agccaaaacc ttcggttgtc agattgatgc
    23941 cgttgactta aaccccaagg ttgtcaaaga agcacaagaa agggttactg ttgaggggtt
    24001 aacccgtcac attacagtat cccaagccaa tgctttatca ctaccatttc cagataacca
    24061 ttttgatatt gtgattaatg aagccatgtt aaccatgttg aacgccaaag ccaaggagaa
    24121 ggcactcaat gaatattggc gtgtgttaaa accaggcggg cgacttttga cacatgacgt
    24181 agcttatgaa gatccaaaaa caagacaagt acttgaagag ttacgtcaaa cgattcatgt
    24241 caatgttgct cccttgtcac taacaggttg gcaagcctta tatcaagaac acggtttttc
    24301 tcaagtgact tatcaccatg ggaaaatgtc tttgatgaca cctcgtggaa tgatcaagga
    24361 tgaaggtctt atgaacaccc tgaacattat gagacgaggc cttaagaaag agaacagaca
    24421 acagttttta aagatgcgtc gtttttttac cagaacaggc aaaaatctct gctatattgc
    24481 tgtaaaaagt gtaaaataaa ccttagacca acctgtccat acaactaaac agaccataga
    24541 ggagttcgaa gatgtcttat atcaataata ttgagcacgc gaaggttctt gatttgacgc
    24601 aagaagtaat gattgagcaa gatcaaatgt taagtagaac actagtccaa aggcaagacc
    24661 taggaataac agttttttcc ttagataagg gccaagaaat tgggcgacac tcatcaccag
    24721 gagatgccat ggttactatc ttaagtggct tagcagaaat caccattgat caagagacct
    24781 accgggttgc tgaaggtcaa accattgtga tgccagctgc tattcctcat gccttatatg
    24841 cagtagaagc ctttcaaatg cttttagtag tagtaaaacc ggaggcttaa catgattgaa
    24901 aatctctctc aactggtgca atatcatgat catcaggtgg ctagccgatc cttgagccgt
    24961 ctactgggat tatctcaatc tattgtttta tatgccatgg cccaaggaga aagcattagt
    25021 caagagacta gtccaagaga taaagtaata cttgttttag aaggtcagct cattttcgat
    25081 ttagaggatc aaaaacaggt cctaactcaa gaaagtctca tagccatacc tgcccaaaaa
    25141 gcccatcatt tagaagctaa aaccgattgt aaactattac aagtgctttt agattaaggg
    25201 cttattgtca actgtattgg gtggcctatg gaaaagcaaa agagaagatg acgctcatct
    25261 tctcttttta cgtattcaaa gcgatgacaa tacgaatttt aaagcttggc cttctcatct
    25321 ccagattaat ccctttagct tagtttattt tagaattcac taaaaaaacc ttaatgagtt
    25381 tcctatctcc ttataggttt ctcattaagg ctttttagac gaaataattg aacgacaggc
    25441 aaaagaaaga tctttttacg agtgataccg ttctcctata gttccataaa tcctcttttt
    25501 gatgttgctg ttagttattc ttagctaaaa gttatattgt tatcaagact tgtcttgtag
    25561 taacatgttt taggagttcc aggataccgt tattctctta gcaagcttaa ctagctagag
    25621 tggagtcact atttataata aactgtcgag cttctcgaca aattggtgat cgtctttgtt
    25681 aatggctagg gtggtaaaac cggtttctgt gggtaatata aagataatat gttgctcaga
    25741 attcttctta tctgttttaa agacgtcaaa gagggatgtc gcagagacat cagctctttc
    25801 aagtgttgtt ggtaactggt aacgttcaaa aacttgaagg aggctatcca agtcctcttt
    25861 tggcaagagt cctttggaaa cagccagttt agcatcaacc accataccga tagcaatggc
    25921 ttcaccgtga tggtaaaaat catggtgaca gagcgattca atcgcgtggc ccaaggtatg
    25981 cccaaaattt aaagacatgc gcaaaccatt ttcaaattca tcttgttcaa caatccgttt
    26041 tttattggaa atggattggt aaatcagacg ttcaagtccc tgcttatcgg tttctttttg
    26101 aaaatcacgt aactgctgaa aaagcggtct atcatgaatg aagccgattt tgagcatctc
    26161 actgatgccg caggagaatt cccgttgcgg taaggtttcc aaaaaggtgg ttgaaataat
    26221 gacagcttct ggagggtaaa tgctgccaat catattggtt agacttttaa aatggactcc
    26281 aactttgccc ccgatactgc tatccacctg actaagtaaa gtcgttggga tttgaatgta
    26341 gggaatacca cggtaatagg ttgcagcgac aaaaccaccc agatccccaa caacaccacc
    26401 accaatagta acaatcatat cttttttgga aaaattttca gcgatgagct ggtcgtagat
    26461 agcactgaca cgtttcaagg atttggattg acctcccggg gggcagacat ggacaaaagc
    26521 gttgtattgc tgagcaaatt cttcaaagag agtctgatac aagtggtaga ccgtttgatc
    26581 ggtgataaac aagagtttac gctgcttgcg ctcaccaagg caatctgcta aggtttttaa
    26641 aacatggtcg gtaaacagga tatcataatc cttgacccga gaatggacat gaagtgtttg
    26701 tggcataagc actcctttca tcagtactag gtaacagaga taacgcagtg atatttagag
    26761 cttaatcttg ccaaagttcc tgccccaact gttctaattg cttataatca atttgctggg
    26821 cagcatctga cagcgcatgg tcgggatcag gatgaacctc catcatgatg ccattggcac
    26881 cgacagcctt cgctatctta gcagcaggta agagtaaatc tcgtctgcct gtcgaatgag
    26941 acacgtccac aataatcggt aaatctgtct tttgttgaat aatagggact gccataatat
    27001 caagcatatt tctagtctcc acatcgtagc cacgaacgcc acgttcgcat aagataatgt
    27061 tagattttcc agtgtcttgt aagtaggaaa gggcgcccaa atattcctcg atagtagcca
    27121 tcaagcctcg tttaaacaaa ataggcttat cgatgtgtga caagattttt aaaaattcaa
    27181 aattttgcat gttgcgggca ccaacctgaa tgacatcgag atagtcatag gcttcttcta
    27241 attggcgctc ggacatgatt tcactgaccg atagtaagcc aaattcctga caaacctcat
    27301 gcaagtaacg aattccttgc aggccaaggc cttgaaaaga agcagcagat gttcttggtt
    27361 tataggcgcc tcctctaaaa tagttgtaac caagtttttt tgcacttgaa gctgctaatc
    27421 taatgtgatc ataagattca atggaacaag gtccaacaat aaaattattc ttagaacaag
    27481 tcttattttg aaagtcagac ataatgtcaa tcattcgttt gattccttta ctaattctgt
    27541 atggcgtgct ctatgggata acgtcttatt taggaataca aaatctcgtt gttatccatg
    27601 aagcaccctt agcctgtgaa atgataccat aaacaagatg aaatagcaag taaaccctag
    27661 attgtctatg ttcttagtca tttttattaa aaaaggcttg ctagctataa aaaaatactg
    27721 ccccaaatct tattaggaca gtatttttta tagatgccac caccgagaat cgaactcgga
    27781 acgaagcatt accattgctt tattatacca tttaactaca gcggctacct ttttataata
    27841 ctataagtta ttaattgatg caagcttttc ttaaaaatat ttccttaatt gtttatcatc
    27901 aaaatgagag taccagttat taataacatg gcacaagagg cctgccttat ggtataatgg
    27961 taaagatgat ttcagaaaga ggaagctaag catgtcttat gaaaaagaat ttttaaaaga
    28021 ttttgaagac tgggttaaaa ctcagattca agtcaatcag ctagccatgg caaccagtca
    28081 agaagttgcc caagaagatg gcgatgaaag ggctaaagat gcctttattc gttatgaaag
    28141 taagttagac gcctacgaat ttttactagg taaatttgac aattataaaa atggtaaagc
    28201 ctttcacgat attccagatg agttatttgg agcaaggcat tattaaggac aaggagacac
    28261 gaaatagtgg caaaacaaaa aaatcgtaaa aaagcattga aattagaaat gaagcgtaat
    28321 ggcttgttaa aaaaagcagg tcaagttttt gataaggcag ttgaaagtgt tgaaacagct
    28381 gtggataaaa ccatttctgc tggtaaaaat cttgtggaaa aaggcagcca aacggttgga
    28441 aacctgacag caagcaagga aagacttact cttgatgagt ttgcagggca gccagttgta
    28501 gctggtatcc gctctgacct tgtggaaacc ctatatgcag aaggcatcca ttcagcgcaa
    28561 gcttttaagg agtggacaga aaaagacctc cttgctctta agggcattgg cccagcaacg
    28621 gttaaaaaat tggtcgaaaa tggcgcaagc tttaaaaaat agtcatcttc tcgttgctta
    28681 atggctataa atttgttaga atattagcaa tcagaggtgc attgagttgg tctgttacag
    28741 agagcggtgg tgttgagaaa tccgcacaga tgtcaaggct agtagctgag gatcagaaat
    28801 cagatatgaa aagtagcaga cccattttat ggtttgaaac agtggtggta ccgcggaaat
    28861 cgtaacacga aattcgtccc tgtctaggtt atctaggcag ggattatttg gtttcatttg
    28921 aaagaagatg atggcttatt gttaaaatgt tgttagaagt atataataga aaaactttgc
    28981 ccaaagtgga aacagaacgg ctgctattac gtcaacgaaa aggggacaag gcattgttcc
    29041 agaagcggct tcagctctga ttgaaattgg ttttacctta cttgatttgc ataaaattga
    29101 gctgggttgc tacgactaca acaagcaaag tcaggcagtg gctcgcaaat taggctttac
    29161 ct
//
LOCUS       DAUCLR010000014        28670 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.014, whole genome shotgun sequence.
ACCESSION   DAUCLR010000014 DAUCLR010000000
VERSION     DAUCLR010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28670
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.014"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(33..1028)
                     /locus_tag="VGT87_000487"
     CDS             complement(33..1028)
                     /locus_tag="VGT87_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEQ3813370.1"
                     /translation="MQRKVTIKDIAELAKTSKTTVSFYLNGRFDKMSEETKNRIKSTI
                     EATNYKPSIAARSLNAKSTKLIGVVIGDITNSFSNQIVKGIESKAQEFGYQIIIGNSN
                     YDPSREDELIEKMLNLGVDGFIIQPTSNFRKYSRIIDIKKKKVVFFDSQLYEHRTNWV
                     KTNNYDAVYDTIQQCIDKGYEHFIMITGNPNLLSTRIERASGFIDVLEANHLTHQEMI
                     IDENQTSSEAIAQFLQGSLTKKSLVFVPNCWALPKVFTAMKSLKFNIPEIGLVGFDNI
                     EWTKFSSPTLTTIIQPAYEEGEQATKILIDDIEGHSQEAKQQIFDCQVNWQESTF"
     gene            complement(1092..2999)
                     /locus_tag="VGT87_000488"
     CDS             complement(1092..2999)
                     /locus_tag="VGT87_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEQ3813371.1"
                     /translation="MSLAFARFKETVNPDFCRNYLLDYQADSYADRKRIADLLLTNTF
                     LFEDNWDMEPCHIPYHLDPITWQEAVTDDPEWNFMLNRQTYLQTLILVYLVERDERYL
                     LKAKSFILNWIESAIPLDPKGLATRTLDTGIRCFAWVKCLIYLNLSDALTKQEERLIL
                     ASLEKQLHFLHANYLDKYSLSNWGILQTTAILLADAYFGSDLDIAAATAFARKELTQQ
                     IALQILEDGSQFEQSTMYHVEVLKALLELTALVPDYLPQLRPTLLAMSDYLLKMTGPD
                     HKQIPLGDSDFTDTRDILTLAATILEEPHLKAAAFPTLDIDSLLLLGEKGVHTFEQLP
                     VQTLPTFAHHFEHSGHITINQENYYLFFKNGPIGSSHTHSDQNSLCLYYKGQPLFCDA
                     GRYTYKEEPLRYALKSASHHSTAFLEEQLPEQIDSSWAYLSYPKSNYCHLRQNGHVYF
                     IEGSYQTQFSDRNNYQHDRQILILPPGILLIIDTIQAQGNHCLVSQFILDNHLDVKTD
                     HLSDLRLISDCPFTIEETILSKKYNQYLTSHKFIKRKPFKDKGCTSTLLVPDDTKVTP
                     LTPLQTGKRNPIETALSWHLKGKQFDYSICVLQEDLIKGEKLVLLNGHKIRGKVVVIN
                     HITNEIIRLKH"
     gene            complement(3086..3907)
                     /locus_tag="VGT87_000489"
     CDS             complement(3086..3907)
                     /locus_tag="VGT87_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEQ3813372.1"
                     /translation="MTGSNKLTKKDYLKTALRAFFLQNGFNYSNYQGIGYANVIYPAL
                     KKHFGSDKKGLYQALEDNCEFYNTNPHFFPFITSLHLVMLENDRPEEETRNIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASLASDGLVLGPIFFFLAMNIILTAIKIGSGLYGYK
                     VGTSFIDKLSEQMAVVSRMANIVGVTVIAGLAATSVKITVPITFAAGKVDAANTAQKF
                     VTIQGMLDKIAPALLPALFTLLMYYLIKNKKWTTYKLVLLTVLIGIIGSWLGILA"
     gene            complement(3894..4676)
                     /locus_tag="VGT87_000490"
     CDS             complement(3894..4676)
                     /locus_tag="VGT87_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEQ3813373.1"
                     /translation="MDINLLQALLIGLWTAFCFSGMLLGIYTNRCIILSFGVGMILGD
                     LPTALSMGAISELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVTPEAALALST
                     PIAVAIQFLQTFAYTAFAGAPETAKKQLQKGNIRGFKFAANGTIWSFAILGLGLGLLG
                     ALSMDTLLHLVDYIPPVLLNGLTVAGKMLPAIGFAMILSVMAKKELIPFVLIGYVCAA
                     YLQIPTIGIAIIGIIFALNEFYNKPKQVDATTVQGGQQDDWI"
     gene            complement(4695..5183)
                     /locus_tag="VGT87_000491"
     CDS             complement(4695..5183)
                     /locus_tag="VGT87_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEQ3813374.1"
                     /translation="MTQPNIIMTRVDERLIHGQGQLWVKFLNCNTVIVANDAVSEDKI
                     QQSLMKTVIPSSIAIRFFSIQKVIDIIHKASPAQNIFIVVKDLQDAKRLVEGGVPITE
                     INIGNIHKTDDKVAITQFISLGETDKSAIRCLAHDHQVVFNTKTTPAGNSASDVDILD
                     YI"
     gene            complement(5219..6418)
                     /locus_tag="VGT87_000492"
     CDS             complement(5219..6418)
                     /locus_tag="VGT87_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEQ3813375.1"
                     /translation="MTRSLKTIALEPIKQPERFTKEDFLSQEDITQALDLALKQVRLN
                     MDYFKEDFPTPATKDNQYAIMDNTEWTNAFWTGCLWLAYEYSGDDAIKALAQANDLSF
                     LDRVTRDIELDHHDLGFLYTPSCMAEWKLLKTPEAREAALKAADKLVQRYQDKGGFIQ
                     AWGELGKKEDYRLIIDCLLNIQLLFFASQETGDNRYRDMAINHFYASANHVIRDDASA
                     YHTFYFDPETGDPVKGVTRQGYSDDSAWARGQAWGIYGIPLTYRFLKEPELIQLFKGM
                     THYFLNRLPKDQVSYWDLIFGDGSEQSRDSSATAIAVCGIHEMLKTLPDHDPDKKIYE
                     AAMHSMLRALIKDYANKDLKPGAPLLLHGVYSWHSGKGVDEGNIWGDYYYLEALLRFY
                     KDWNPYW"
     gene            complement(6418..6855)
                     /locus_tag="VGT87_000493"
     CDS             complement(6418..6855)
                     /locus_tag="VGT87_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEQ3813376.1"
                     /translation="MIAIIVMGHGHFASGIVSSLELIAGPQEKVTAIDFTAEMTAADV
                     QDQLSRALIPEEETLVLCDLLGGTPFKVAATLMESLPNTTCNVLSGLNLAMLIEASFA
                     RQTAASFDDLVSGLITCSKEGIVDWKTLSQQEDGATDDELGGI"
     gene            7191..7985
                     /locus_tag="VGT87_000494"
     CDS             7191..7985
                     /locus_tag="VGT87_000494"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEQ3813377.1"
                     /translation="MENMFSLQGKIALITGASYGIGFEIAKAYAQAGATIVFNDIKQE
                     LVDRGMAAYRELGIEAHGYVCDVTDEAGIQQMVSQIEDEVGVIDILVNNAGIIRRTPM
                     LEMAAEDFRQVIDIDLNAPFIVSKAVLPSMIAKGHGKIINICSMMSELGRETVSAYAA
                     AKGGLKMLTKNIASEFGEANIQCNGIGPGYIATPQTAPLRERQSDGSRHPFDQFIIAK
                     TPAARWGTTEDLAGPAVFLASDASNFVNGHILYVDGGILAYIGKQP"
     gene            8010..8651
                     /locus_tag="VGT87_000495"
     CDS             8010..8651
                     /locus_tag="VGT87_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEQ3813378.1"
                     /translation="MKIALINENSQAAKNGIIYDALTSVTDKHGYQVFNYGMYGTEGE
                     SQLTYVQNGLLASILLTTKAADFVVTGCGTGVGAMLALNSFPGVTCGFASEPTEAYLF
                     SQINGGNALSIPFAKGFGWGAELNLTLIFERLFAEPMGGGYPKERAIPEQRNARILSD
                     LKKITYRDLLAIVKDIDQDFLKETISGAHFKEYFFANAEPSELVTYLKSVLEQ"
     gene            8680..9681
                     /locus_tag="VGT87_000496"
     CDS             8680..9681
                     /locus_tag="VGT87_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEQ3813379.1"
                     /translation="MSKLLLVGEPLIRVSPNQFQPLTNACDAQLFFGGSEVNIARTLD
                     GFGLEARLFTALPDNPVGHAFHQFLKQSGVDMSLTAWQGHRVGLYYLENGFGCRASQV
                     YYDRCGSSFSALDKDSLDLAAIFEGIRHFHFSGISLALGKKTQDLIEVLVREAKKRDI
                     CVSFDLNFRSSMIAVADAKRLFSHFAQYADIIFGMEPLLLDSDDFDMFDRNKADATMI
                     RERLAGLYQRYQLQAIYHTERSNDAQGSNRFKAYAYDGQFYESCEVTTPVLQRVGSGD
                     AFVAGLLYQLLEGNEKQRNLDFAVATASLKCTVAEDQLYHSVQQVEAVLANQRDVQR"
     gene            9686..10321
                     /locus_tag="VGT87_000497"
     CDS             9686..10321
                     /locus_tag="VGT87_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEQ3813380.1"
                     /translation="MGKIEILTKLKANRLVLVVRGESSEEALACSLASIEGGIKTIEV
                     TYTNPFASEVIGQLAERFKEDPEVLIGAGTVLDDVTARQAILAGAQFIVGPNFNRAVA
                     LICHRYSIPYLPGCMTVNEVVTALESGVDMVKIFPGSTVGMSFIRAIKSPLPQVEVMV
                     TGGVSLDNLKDWLAAGVDVLGIGGEFNQLASQKQYNLITKKAAHYIKSLRQ"
     gene            10618..11268
                     /locus_tag="VGT87_000498"
     CDS             10618..11268
                     /locus_tag="VGT87_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEQ3813381.1"
                     /translation="MIKGIIFDMDGVLFDTEPFYLRRREDFFKTKGIPIDHLNSKDFI
                     GGNLQELWKELLGKNRDDAIVKAITADYDAYKQAHKPPYQKLLITEVNSCLEQLKKQG
                     IKLAVASNSKRQDVLLALETTQIKDYFEIILAREDVSRGKPYPDIYNKAVQKLGLQKK
                     QLLVVEDSQKGIAAAKAANLTVFAITDYRYGIDQSQADHKIDHLGQLCVKIGCFGS"
     gene            11965..13089
                     /gene="queG"
                     /locus_tag="VGT87_000499"
     CDS             11965..13089
                     /gene="queG"
                     /locus_tag="VGT87_000499"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEQ3813382.1"
                     /translation="MTIKAEIKALAKEIGISKIGFTTADNFDYLEKSLRASVEEGRNS
                     GFEHKVIEDRISPERLLESAKTIISIGVAYPHKLPQQPQKTPYKRGKITPSSWGFDYH
                     YVVGEKLDRLSKGIEELCRDFPLQQKAMVDTGALVDTAVAQRAGIGFIGKNGLVISKE
                     YGSYMFLGELITNLEIEPDKPVDYDCGDCRRCLDACPTSCLIGDGSMNAKRCLSFQTQ
                     DKGMMDIEFRKKIKTVIYGCDICQICCPYNKGINNPPATEIDPELAQPELIPFLSLSN
                     GQFKEKFGMIAGSWRGKNILQRNAIIALANAHDKTAVVKLIEIIDKNNNPIHTATAIW
                     ALGEIVKKPNDEILAFMSHLTLKDEDSQKELELIRHKWQF"
     gene            13158..14256
                     /gene="prfB"
                     /locus_tag="VGT87_000500"
     CDS             join(13158..13229,13231..14256)
                     /gene="prfB"
                     /locus_tag="VGT87_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889043.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEQ3813383.1"
                     /translation="MEVAEIRQKIVENKEKLTSFRRSLDLDRLEEEIALLENHMTEPD
                     FWNDNIAAQKTSQELNELKGKYDTFHNMQELSDETELLLEMLDEDDSLKEELEENLMQ
                     LDKIMGAYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDLLLRMYTRFGNANGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFASVE
                     VMPELDDTIEVEVRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVSSTVDRT
                     QYGNRDRAMKMLQAKLYQLEQEKKAQEVDALKGDKKEITWGSQIRSYVFTPYTMVKDH
                     RTNFELAQVDKVMNGEINGFIDAYLKWRIED"
     gene            14275..14967
                     /gene="ftsE"
                     /locus_tag="VGT87_000501"
     CDS             14275..14967
                     /gene="ftsE"
                     /locus_tag="VGT87_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEQ3813384.1"
                     /translation="MALIEMSGVTKKYRRSTTALRDVNVSVNQGEFVYLVGPSGAGKS
                     TFIKLLYREEQLTTGKLYVGEFNLTKLKARDVPILRRHIGVVFQDYKLLPRKTVFENV
                     AYAMEVIGEKRRHIKKRVPEVLDLVGLKHKMRSFPSQLSGGEQQRVAIARAIVNNPKL
                     LIADEPTGNLDPEISWEIMQLLERINVQGTTILMATHNSHIVNTFRHRVVAIEDGRIV
                     RDEEKGDYGYDD"
     gene            14960..15889
                     /locus_tag="VGT87_000502"
     CDS             14960..15889
                     /locus_tag="VGT87_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ3813385.1"
                     /translation="MIRYFFRHIWESIKNLKRNFWMTFASVSMVAVTLTLVGVFAATL
                     LNIQRVASGVENNVHINTYLQVDSTDAAKVIQNTAGEPVNNDNYHSVYDKIAQIKGVK
                     KITFSSKDEQLKKLQETLGDVWNMYDQDTNPLQDIYLIETQTPKQVKAITKKIRTIEG
                     VEAADYGGINSDKLFKFSTLIQTWGLIGTAMLLFVAVFLISNTIRMTIMSRKRDIEIM
                     RLVGAKNSYIRGPFFFEGAWVGLLGAVLPSLLIYYGYDLVYKHFAQELQRNNLSMYPL
                     DPYVYYLIGALFVIGIMIGSLGSVLSMRRYLKF"
     gene            complement(16198..16896)
                     /locus_tag="VGT87_000503"
     CDS             complement(16198..16896)
                     /locus_tag="VGT87_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEQ3813386.1"
                     /translation="MPFIFRHSFFNKALIFWYTILMKIYKTINHIAGENTYYLVNDQA
                     VILIDPGSNGQEIIAKIKSFEKPLVAILLTHTHYDHIFSLDLVRDAFDHPPVYVSEKE
                     AAWLSSPDDNLSGLGRHDDIITVIARPAENFFKLKQPYQLNGFEFTVLPTPGHSWGGV
                     SFVFHSDELVVTGDALFRETIGRTDLPTSNFEDLITGIRQELFTLPNHYRVYPGHGPS
                     TTICHEKNANPFFH"
     gene            17064..17828
                     /locus_tag="VGT87_000504"
     CDS             17064..17828
                     /locus_tag="VGT87_000504"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HEQ3813387.1"
                     /translation="MSKVAIVTGAGQGIGFAIAKRLHADGFKIGILDYNEETAQAAVD
                     KLSPEDTVAVVADVSKRDQVFDAFQKVVDTFGDLNVVVNNAGVAPTTPLDTITEEQFE
                     KAFAINVGGTIWGSQAAQKHFRELGHGGKIINATSQAGCEGNPNLTVYGGTKFAVRGI
                     TQTLAKDLASEGITVNAYAPGIVKTPMMFDIAHEVGKNAGKDDEWGMEQFAKDITLKR
                     LSEPEDVANAVGFLAGDDSNYITGQTIVVDGGMVFH"
     gene            17936..20437
                     /locus_tag="VGT87_000505"
     CDS             17936..20437
                     /locus_tag="VGT87_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEQ3813388.1"
                     /translation="MFCFIDIACYNRLTMTQKKLRKYAVVDLEATGAGPNASIIQVGI
                     VIIQGNKIIDSYETDVNPHESLDEHIVHLTGITDKQLAKAPDFGQVAHHIYQLIEDCI
                     FVAHNVKFDANLLAEALFLEGYELTIPRVDTVELAQLFFPRFEKYNLGHLSRQLNIDL
                     AEAHTAIADARATAILFLRLLQKIESLPIECLESLLVYSDSLLFETAMVIQEGLAKAK
                     PYDPNKYIKIRQILLPKGPKALKPYQISKSFPINMALLGLEERPKQTQFAQLIDEDYH
                     QGVASFIEAQTGIGKTYGYLLPLLAKEDQNQIIVSVPTKLLQDQLMAGEVAAIQEQFH
                     IACHSLKGPANYLKLDSFADSLDQNDQNRLVNRYKMQLLVWLLETKTGDLDEIKQKQR
                     FAAYFEQLKHDGDIKQSSEFYNYDFWRVNYEKAKTARLLITNHAYFLHRVQDDKDFAR
                     NKVLVFDEAQKLMLQLDQLSRHQLNLTVFLQTIQAKLSNPLPLLEKRLLESLSFELGQ
                     VSSDYYQNKEHQLAHDWSRIAGYAKELTGADYQELQAFFATSDGDYWLSSEKQEEKRL
                     TYLNSASKAFIHFQQLLPETVKTYFVSATLTISSEVTLADLLGFEEYLYHVIEKDKKQ
                     DQLVLVDQEAPIVTEVSDQIYVEAIAKRIESLKQEGYPILVLFNSKKHLLLVSDYLDQ
                     WQVPHLAQEKNGTAYNIKKRFDQGEQTILLGLGSFWEGVDFIQADRMITLIARLPFDN
                     PEDFFVKKMSHYLLEKGKNPFRDYFLPMTILRLKQAIGRTMRRQDQKSVVIILDRRLL
                     TKSYGQVILEGLGQEFLISQQNFHDCLVETDCFLI"
     gene            20772..21965
                     /locus_tag="VGT87_000506"
     CDS             20772..21965
                     /locus_tag="VGT87_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEQ3813389.1"
                     /translation="MPKLSKRVLEMKESVTLAAGARAKALKAQGRDVLNLTLGEPDFF
                     TPKHIQDKAIESIQNGTASFYTNASGLPELKAAIATYLKNQYGYHLSPDQIVAGTGAK
                     FILYAFFMAVLNPGDQVLIPTPYWVSYSDQVKMAEGQPIFVQGLEENQFKVTVDQLER
                     ARTSKTKVVLINSPSNPTGMIYGAEELRAIGEWAVHNDILILADDIYGSLVYNGNQFV
                     PISTLSEAIRRQTITVNGVAKSYAMTGWRVGFAAGEPEIISAMSKIIGQTTSNLTTVS
                     QYAAIEAFCGSQSSLEEMRLAFEERLNITYPLLCQVPGFEVVKPQGAFYFFPNVKKAM
                     EMTGFSDVTSFANAILEEVGLAVVSGAGFGAPENVRLSYATDIETLKEAVRRLHVFME
                     SNEIN"
     gene            21986..23332
                     /gene="asnS"
                     /locus_tag="VGT87_000507"
     CDS             21986..23332
                     /gene="asnS"
                     /locus_tag="VGT87_000507"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEQ3813390.1"
                     /translation="MSKKLISIVDVKDYVGQEVTIGAWVANKSGKGKIAFVQLRDGSA
                     FFQGVAFKPNFIEKYGEESGLEKFDVIKRLNQETSVYVTGIVKEDERSKFGYELDITD
                     LEIIGESHEYPITPKEHGTDFLMDNRHLWLRSRKQMAVMQIRNAIIYATYEFFDQNGF
                     IKFDSPILSENAAEDSTELFETDYFGKPAFLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSFLSHEESLDLQEAYVKALIQGVLDRAPQALDILERDVE
                     ALKRYITEPFKRVSYDDAITLLQEHEADEDTDYEHLEHGDDFGSPHETWISNYFGVPT
                     FVVNYPASFKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREDNYDALVAKMDEL
                     GMDKSEYDFYLDLRKYGSVPHGGFGIGIERMVTFVAGTKHIREAIPFPRMLHRIRP"
     gene            23747..24637
                     /gene="rapZ"
                     /locus_tag="VGT87_000508"
     CDS             23747..24637
                     /gene="rapZ"
                     /locus_tag="VGT87_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002282100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEQ3813391.1"
                     /translation="MSDKHINLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALVPK
                     FLELIEQTNENRRVALVVDMRSRLFFKEINSTLDSIESNPSIDFRILFLDATDGELVS
                     RYKETRRSHPLAADGRVLDGIRLERELLSPLKSMSQHVVDTTKLTPRQLRKTISDQFS
                     EGSNQASFRIEVMSFGFKYGLPLDADLVFDVRFLPNPYYKVELREKTGLDEDVFNYVM
                     SHPESEVFYKHLLNLIVPILPAYQKEGKSVLTVAIGCTGGQHRSVAFAHCLAESLATD
                     WSVNESHRDQNRRKETVNRS"
     gene            24634..25611
                     /locus_tag="VGT87_000509"
     CDS             24634..25611
                     /locus_tag="VGT87_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEQ3813392.1"
                     /translation="MKNPKMTVIGGGTGISIILKSLRNEAVDITAVVTVADDGGSSGE
                     LRNAMQLAPPGDLRNVLLAMSDMPKFYERVFQYRFNENDGALAGHPLGNLIIAGISEM
                     QGSTYNAIQILTKFFHITGKIYPSSEQALTLHAVFKDGHEVAGESSIAKYQGMIDHVY
                     VTNTYNDQKPQASRKVVEAILESDMIVLGPGSLFTSILPNLVIPEIKEALRQTKAEVV
                     YICNIMTQYGETEQFSDADHVAVLNQHLGRDLIDTVLVNVAKVPQAYMNSNKFDEYLV
                     QVDHDFAGLCRAAKRVISSYFLRLENGGAFHDGNLVVEELMNLVRIVKQ"
     gene            25608..26519
                     /gene="whiA"
                     /locus_tag="VGT87_000510"
     CDS             25608..26519
                     /gene="whiA"
                     /locus_tag="VGT87_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEQ3813393.1"
                     /translation="MSFTTKVKEELIHLSTGDNNELAAIIKLSGSLGLAHQSLHLSIT
                     TENAKIARYIYSLIEDAYVIVPEIRYHQKTNLRKNRVYTVYVEQGVETILADLKLADS
                     FFGLETGIEPQVLSDDNAGRSYLKGAFLAAGSIRDPESGKYQLEIYSVYLDHAQDLAQ
                     LMQKFMLDAKTIEHKSGAVTYLQKAEDIMDFLIIIGAMSCKEDFEAIKLLREARNDIN
                     RANNAETANIAKTISASMKTINNIIKIMDTIGLESLPIELQQVAQLRVKHPDYSIQQV
                     ADALEFPITKSGVNHRLRKINKIADDL"
     gene            26652..28049
                     /locus_tag="VGT87_000511"
     CDS             26652..28049
                     /locus_tag="VGT87_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HEQ3813394.1"
                     /translation="MACTTILVGKKASYDGSTMVARTEDSQNGDFTPKKMIVVKPEDQ
                     PRHYRSVQSSFEMDLPDNPMTYTSVPDALGKDGIWAEAGVNEANVAMSATETITTNSR
                     VLGADPLVASGIGEEDMVTLVLPYIRSAREGVLRLGAILEDYGTYESNGVAFSDEHDI
                     WWLETIGGHHWIARRVPDDAYVTNPNQFGIDHFEFNNPEDYLCSADLKDFIDTYHLDL
                     TYSYEHFNPRYAFGSQRDKDRHYNTPRAWIMQKFLNPEIVQDPCSFALAWCQKPYRKI
                     TVEDVKYVLSSHYQDTVYDPYGSEGTPVSKKVFRPIGINRTSQTAILHIRPNKPQEIA
                     AIQWMAYGSMPFNTMVPFFTQVKTIPDYFANTYENVSTDNFYWTNRLIAALADPHYNH
                     HETDLDNYLEETMAKGHAMLHAVEAQLLAGETVDLEEENQKMSDYVQGETQTLLNKIL
                     FDASNLMTNRFSLSD"
     gene            28201..>28670
                     /locus_tag="VGT87_000512"
     CDS             28201..>28670
                     /locus_tag="VGT87_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEQ3813395.1"
                     /translation="MKKKILLMMSLISVFFAWQLTQAKQVLAEGKVKVVTTFYPVYEF
                     TKGVIGNDGDVSMLMKAGTEPHDFEPSTKDIKKIQDADAFVYMDDNMETWVSDVKKSL
                     TSKKVTIVKGTGNMLLVAGAGHDHHHEDADKKHERNKHSEEGHNHAFDPHVWLSP"
BASE COUNT         8755 a         5044 c         6173 g         8698 t
ORIGIN      
        1 tacaacgata aggactattg gtatgcctga cattaaaaag ttgattcttg ccaattgact
       61 tgacagtcaa aaatttgttg tttggcttct tgactgtgac cttcaatatc atcgattaaa
      121 atcttagtag cctgttcgcc ttcttcatag gcgggttgga taatagttgt tagtgttggt
      181 gaagaaaatt ttgtccactc aatgttatca aatcctacta gtccaatctc tggtatatta
      241 aatttaagag acttcatagc tgtaaatact tttggaagag cccaacaatt ggggacaaaa
      301 accaaactct ttttagtcag tgatccttgc aaaaactgag caattgcctc tgaagaagtc
      361 tgattttcat caataatcat ttcttgatga gtaagatgat tagcttctaa cacatcaatg
      421 aagcctgaag cgcgttcaat acgagtgcta agtaaattgg ggttacccgt gatcatgata
      481 aaatgttcat aacctttatc gatacattgt tgaatggtat cataaacagc atcataatta
      541 ttagttttga cccagtttgt gcgatgttcg taaagttggc tatcaaaaaa gaccactttt
      601 ttcttcttaa tatcgataat gcgagaatac ttcctgaaat tagaggtagg ctgaatgata
      661 aagccatcca ctccgaggtt cagcatcttt tcaatgagtt catcttcgcg agaagggtcg
      721 tagttgctgt ttccgatgat aatttggtaa ccaaattcct gggctttgct ttcaattccc
      781 ttaacaattt gattggaaaa actattggta atatcaccaa taaccacgcc aatgagttta
      841 gtagatttgg cgtttagact tctggcagca atgcttggtt tataattggt agcttcaatc
      901 gtgcttttaa tgcggttttt agtttcttca gacatcttgt caaacctacc atttaagtag
      961 aaagaaactg ttgtttttga agttttcgct aattcagcaa tatctttgat ggttactttg
     1021 cgctgcattg catacactcc ttaaaaatag taatggttag tcatattata acataaggac
     1081 tcgtaaaaga tttagtgttt caatcgaata atctcattcg tgatatggtt gataacaacc
     1141 acctttcctc tgattttatg accatttagg agaactagtt tttctccttt aattaagtcc
     1201 tcttggagaa cgcagatact gtagtcaaac tgctttcctt ttaaatgcca ggaaagggcg
     1261 gtctcaattg ggttgcgttt gcctgtttga agtggtgtta agggagtaac tttggtatcg
     1321 tcagggacta acagggtact ggtgcaaccc ttgtctttaa agggttttcg tttgatgaat
     1381 ttgtgactag tcaggtattg gttgtatttt tttgacaaaa tcgtttcttc gatagtgaaa
     1441 gggcagtcac tgattagtct gaggtcacta aggtgatctg tctttacgtc taagtgatta
     1501 tcaagaataa attgactgac aagacagtgg ttaccttgag cctgtatcgt atcaatgata
     1561 agcaaaattc ctggtggtaa gatgagaatt tgtcggtcat gttggtagtt gttcctatcg
     1621 gagaattgcg tctggtagct accttcaata aagtaaacgt gtccattttg tcgaaggtga
     1681 caataattgg atttggggta agaaaggtaa gcccaggagc tatcaatctg ctcaggtaat
     1741 tgttcttcta agaaggcggt agaatgatgg ctagcgcttt ttagggcata gcgtaatggt
     1801 tcttctttgt aagtgtatcg cccagcatca cagaaaagtg gttgtccttt ataatagaga
     1861 cagagactat tttggtcgct atgggtatgg gaactaccga tgggcccatt tttgaaaaaa
     1921 agataataat tttcttgatt gatggtaatg tgcccggaat gttcaaagtg atgcgcaaaa
     1981 gttggtaagg tttggacggg taactgttca aaagtgtgga cacctttttc tcctagaagg
     2041 agaagactat cgatatcaag tgtgggaaaa gcagcagctt ttaagtgtgg ttcttcaagg
     2101 atagtggctg ctagtgttag aatatctctg gtatcggtaa agtcactgtc tcctaagggg
     2161 atctgtttgt ggtcagggcc agtcattttg aggagataat cactcatggc aagcaaagta
     2221 ggtctaagct gtggaagata atcagggact agagcagtta attccagaag ggctttgagt
     2281 acttccacat gatacatggt tgattgttca aattgactgc cgtcttcgag aatttggagt
     2341 gcgatttgtt gcgtcaattc ttttctagcg aaagctgttg ctgcagcaat atcaaggtca
     2401 gaaccaaagt aggcatcagc taataaaatc gcagtggttt gcaagattcc ccaattgctg
     2461 agactgtatt tatcaaggta gttggcatgg agaaagtgaa gttgtttttc cagggaagct
     2521 aggatgagcc tttcctcttg cttagttaag gcgtcagaaa gattaaggta aatcaaacat
     2581 ttgacccaag caaaacaacg gatgccagta tctaaggttc tagtagctaa ccccttaggg
     2641 tctaaaggaa tagctgattc aatccaattg aggatgaaac ttttggcttt taggagatac
     2701 cgttcatctc tttcaacaag gtagacgagg attaatgttt ggagataggt ctgtcgattt
     2761 aacataaaat tccactcagg atcatctgtg acagcctctt gccaagtaat gggatctaag
     2821 tggtaaggga tgtggcaggg ctccatatcc caattatctt caaataaaaa ggtattggtt
     2881 aagagtaggt cagctatcct ttttcgatca gcatagctgt cagcctgata gtctagcagg
     2941 taattcctgc aaaagtctgg attgactgtt tctttaaagc gtgcgaaagc aagagacata
     3001 ggacatttcc tttccaaaag aaaagagacc tcatcatgtg cttaggaact atttccctat
     3061 gctcataaca ggtctctgtg aaccattaag ctaaaatacc taaccagcta ccgataatac
     3121 cgataagaac agtgaggaga accaatttgt aggttgtcca ttttttgttt ttaataaggt
     3181 aatacatgag taaggtaaag agagctggta aaagtgcagg agcaatttta tcaagcattc
     3241 cttgaatagt cacaaatttt tgtgctgtat tggccgcatc caccttgcca gccgcaaaag
     3301 taatgggaac agttatttta acagaagtag ccgctagtcc agcgataacg gtaacgccga
     3361 caatatttgc catgcgcgac acaaccgcca tttgttcgct tagtttatca ataaagcttg
     3421 tgccgacctt gtagccatag agaccagaac cgattttaat agcggttagg ataatattca
     3481 tggctaggaa gaagaaaatt ggacctaaaa ctaaaccatc actggctaag gaagctgcga
     3541 tcgttgaaaa gagtggcgct aagcaaaatt gtgataagga gtcaccgata ccagctaaag
     3601 gccccataag tgccattttg atgtttcggg tttcttcttc aggacgatca ttttctaaca
     3661 tgaccaagtg cagactggtg atgaaaggaa agaagtgagg gttggtattg taaaactcac
     3721 agttatcttc taaagcttga tacaagccct ttttatcact gccaaaatgt tttttcaagg
     3781 caggatagat gacattggcg tatccgatac cttggtaatt actgtaatta aacccatttt
     3841 gtaggaagaa agctctcaga gctgttttga ggtaatcttt cttggttaat ttattagatc
     3901 cagtcatctt gttggcctcc ttggacagtt gttgcatcga cttgtttagg tttgttgtaa
     3961 aattcattca aggcgaaaat gataccaata atggcgatac cgatggttgg aatttggagg
     4021 taggccgcac aaacataacc aatcagtaca aaaggaatca actccttctt ggccataaca
     4081 gataagatca tggcaaatcc gatagcaggt aacattttac cagcgacagt caatccattg
     4141 agcaagacag gtgggatata atccactaag tgaaggagag tatccattga caaggcacct
     4201 aataaaccaa ggcctaatcc aaggatagca aaagaccaaa tggtcccatt agcagcgaat
     4261 ttgaagcctc taatattgcc tttttgcaat tgttttttag cggtttcagg agccccagca
     4321 aaagcggtat aggcgaatgt ctgaagaaat tggatggcaa ctgcgattgg tgttgataag
     4381 gctagggccg cttctggggt gactttacca gcactggtaa tagccatcaa ggtaccaaag
     4441 ataccagggc cgataggatt aggtggaacg gtaccaccgg cacccactcc aaatcccata
     4501 taagctaatt cggagatagc tcccatgcta agtgcagttg gcaagtcacc taagatcatc
     4561 cctacaccaa aagacagaat aatacaacgg ttggtgtaga tgccaagtaa cattccgctg
     4621 aaacaaaagg ctgtccaaag accaattaaa agtgcctgta acaaattgat atccatatca
     4681 atctccttta ttcattaaat ataatctaag atgtcaacgt cagaggcgct attgccagct
     4741 ggagttgttt tggtgttaaa aaccacttgg tggtcatggg ctaagcaacg aatagcagac
     4801 ttgtctgttt ctcccagcga aataaactgg gtaatcgcaa ccttatcatc agttttatga
     4861 atattaccga tattgatttc tgtgatggga acaccccctt caactaggcg tttggcatct
     4921 tgcaaatctt tgaccacgat gaagatattt tgagcaggac ttgctttatg aatgatgtca
     4981 atcacttttt gaatggagaa aaaacgaatg gcaatactgg atggaataac tgttttcatg
     5041 agggattgtt gaatcttatc ttcagaaacc gcatcgttag cgacgataac ggtgttgcag
     5101 ttgaggaatt tgacccataa ttgcccctgt ccgtgaatca aacgctcatc aactcgcgtc
     5161 atgataatgt ttggttgtgt cataaaatct cctttttaaa tgtaggtatc ggtgtttgtt
     5221 accagtaagg gttccagtct ttgtagaaac gaagcagagc ttcaagatag taatagtctc
     5281 cccagatatt tccctcgtca accccttttc cagagtgcca agaatagact ccgtgaagta
     5341 gtagaggagc gccaggcttg agatctttgt tagcataatc cttaatcaag gctcttagca
     5401 tgctgtgcat ggcagcttca tatatttttt tatcaggatc atgatctggt aaggttttta
     5461 acatttcatg aataccacac acggcaatgg ctgttgctga actatcacga gactgctcgc
     5521 taccatctcc aaaaattaaa tcccagtagg aaacctggtc tttaggaaga cggttcaaga
     5581 aataatgggt catgcccttg aagagttgaa tgagttcagg ttcttttagg aaacgatagg
     5641 tcaaaggaat accataaatg ccccaagctt gccctctggc ccaagcagaa tcatcgctat
     5701 aaccttgtcg ggtgacccct ttaacaggat cgcctgtttc tggatcaaag tagaaggtgt
     5761 ggtaagcaga ggcgtcatct cggatgacat gattggctga ggcgtagaaa tggttgatcg
     5821 ccatgtcacg gtaacggttg tctccagttt cttggctagc aaaaaagagt aattggatgt
     5881 ttaggagaca atcaatgatt aaacggtaat cttctttttt ccccagttct ccccaggcct
     5941 gaataaagcc gcctttgtct tgatagcgtt ggactaattt gtcggctgct ttcaaagcag
     6001 cttctctagc ctcaggtgtt tttaaaagtt tccactcagc catgcaagaa ggggtgtaaa
     6061 gaaagcccaa atcatggtga tctagctcaa tgtctcttgt tactcgatct aggaaagaaa
     6121 ggtcatttgc ttgagccaga gctttaatag catcatcccc actgtattcg taagctaacc
     6181 aaaggcaacc tgtccaaaag gcattggtcc attccgtgtt gtccataatg gcgtattggt
     6241 tatctttggt agctggcgtt ggaaaatctt ctttgaagta gtccatgtta agccgaactt
     6301 gtttgagggc taagtcaagg gcctgagtaa tgtcttcttg gcttagaaag tcttctttgg
     6361 taaatcgttc aggctgcttg ataggttcaa gagcaatggt ttttaaagat cgtgtcatta
     6421 aatgcctcct aactcatcgt ctgtcgcacc gtcttcttgc tgagaaagag ttttccagtc
     6481 aacaatgcct tctttagaac atgttataag tcctgatact aagtcgtcaa aagaagctgc
     6541 tgtttgtctt gcaaaactag cttcaatcag catggctaag ttaaggccag aaagaacatt
     6601 acacgtcgtg tttggaagag attccattaa ggttgcagcg actttaaaag gggtacctcc
     6661 tagaaggtca caaagaacta aggtttcctc ttctgggatt aaagcacgag ataattggtc
     6721 ctgcacatca gcggcggtca tctcagcagt aaaatcaatt gcagttacct tctcttgtgg
     6781 tcctgcaatt aactctaggg atgagacaat cccacttgca aagtgtccat gtcccataac
     6841 aataatagca atcatgataa aactcctttt tggtttaccg gtttacttaa aaatattgta
     6901 gcgctttcaa aattctttgt caatactttt ttaaaagatt tttgaaaaag aaaaagtgag
     6961 cagttgtgct aaaaaagaat caaaaatggt cttttttgtc actatttaat gtagaaaagt
     7021 cttaatagct attaagtata ggacaagact aaacaagaat gacagtttgg ataagagcac
     7081 tcttttaaat ttttgaaccg ttttcattaa aatgattgac aaaactagtt tactttgtta
     7141 tagtcttggt aaacaggttt acttaattta ataacaaaaa ggagaatcct atggaaaata
     7201 tgttttcgtt acaaggtaag attgccttga ttaccggagc atcctacggt attggttttg
     7261 aaattgccaa agcctatgct caagcgggtg caactattgt gtttaatgac atcaaacaag
     7321 agttagttga tagagggatg gctgcctatc gagaattagg gatagaagcg catggctatg
     7381 tttgtgacgt gactgatgaa gctggtatcc aacagatggt tagtcaaatt gaagatgaag
     7441 taggtgttat tgatattttg gtcaacaatg caggtattat taggcgtacg ccgatgcttg
     7501 aaatggccgc agaagatttt cgtcaagtta ttgatattga tttaaatgct ccttttattg
     7561 tgtcaaaagc tgtattacca tcaatgattg caaagggaca tggtaaaatt ataaatatct
     7621 gttctatgat gagcgaattg gggcgtgaaa cagttagtgc ctacgcggcg gctaaaggtg
     7681 gcttgaaaat gttaaccaaa aatatagcct ctgagtttgg agaagccaac attcaatgta
     7741 atgggattgg tcctggctac attgcaacac cacaaacagc tccgttgaga gagcgacaat
     7801 cagatggtag tcgtcatcct tttgatcaat ttatcattgc taaaacacca gcagcacgtt
     7861 gggggacaac agaagactta gctggtccag ctgtttttct agctagcgat gccagtaatt
     7921 ttgtcaacgg ccatattctt tatgtcgacg gtggcatttt agcttatatt ggaaaacaac
     7981 cttaatcaca cactagaaag agattttata tgaaaattgc attaattaat gaaaatagcc
     8041 aagcggctaa aaatggtatt atttacgatg cccttacctc agtgacagat aaacacggct
     8101 accaagtctt taactatggc atgtatggca cagaaggtga aagccagttg acctatgttc
     8161 aaaatggact cttagcttct attttattga ccactaaggc ggctgatttt gtggtgactg
     8221 gttgtgggac aggtgttgga gctatgttgg ccttgaacag cttcccaggt gttacctgtg
     8281 gttttgcgag tgagccaaca gaagcatatc tcttttctca aatcaatggt ggtaatgcct
     8341 tgtccattcc ttttgccaaa ggttttggtt ggggagcaga gttgaatttg accttaatat
     8401 ttgaacgcct ctttgcagaa ccaatgggtg gtggttaccc taaagagcga gctattcctg
     8461 aacagcggaa tgcacgtatt ttaagtgatt tgaaaaaaat tacttacaga gatttgttgg
     8521 caattgtcaa agacatcgac caagatttcc tcaaagaaac gatttctggc gctcatttta
     8581 aagagtactt ctttgccaat gcggagccgt cagaattagt tacttatttg aaatcagtac
     8641 ttgaacagta attccatttt gacgaggaaa ggaggtcaca tgtctaagtt attattggtt
     8701 ggtgaaccgc tgattcgcgt tagccccaat cagtttcagc ctttgaccaa tgcctgcgac
     8761 gctcagcttt ttttcggcgg ttcagaagtt aatattgcaa gaactctaga cggttttggc
     8821 ttagaagcaa ggctatttac ggccctacca gataatcctg ttggtcacgc ttttcatcag
     8881 tttttgaagc aaagcggcgt ggatatgtcc ttgacggctt ggcaaggtca tcgcgttggg
     8941 ctatattacc ttgaaaatgg ctttggctgt cgggctagtc aggtttatta tgaccgctgt
     9001 ggctctagct tttcggcctt agataaggat agtcttgatt tagctgccat ttttgaaggc
     9061 attcgtcact ttcattttag tgggatcagt ttggcgctgg gcaaaaaaac tcaggacctg
     9121 attgaggtac ttgtgagaga agctaagaaa agggatattt gtgtttcttt tgatcttaac
     9181 tttagatcta gtatgattgc ggtagctgat gctaaacgac tcttttctca ctttgctcaa
     9241 tacgcggata ttatttttgg catggaacct ttgctgttag atagtgacga ttttgacatg
     9301 tttgatagga ataaggcaga tgctacgatg attagagaac gtctggcagg actttatcag
     9361 cgctatcagt tacaagctat ttaccatacg gagcgttcaa atgatgccca aggcagtaat
     9421 cgtttcaagg cttatgccta tgatgggcag ttttacgaat cctgtgaagt gacgacccca
     9481 gtcttgcaac gggttggaag tggagatgct tttgtggcgg gtttacttta tcagttgtta
     9541 gaaggtaacg aaaagcagcg caaccttgat tttgctgtgg caacagctag cctaaaatgt
     9601 acagtagctg aagatcagct ctatcacagc gtccaacaag tagaagcagt cttagctaat
     9661 caacgagatg tccagaggtg atgatatggg aaaaatagaa atcttaacca aacttaaggc
     9721 taatcggctt gtcctggttg tacgaggaga gtctagtgag gaagctttag cttgcagcct
     9781 ggccagtatt gaaggtggca ttaagacaat tgaagtcacc tatactaacc cctttgctag
     9841 tgaggttatc ggtcaattag ctgaacgctt caaagaagat cctgaggtat tgataggtgc
     9901 tgggacagtt ttggatgatg tcactgcccg ccaagctatt ttagcaggag cacagtttat
     9961 tgttggtcct aatttcaatc gagcagtggc tttgatttgt catcgttata gcatacctta
    10021 tttaccaggt tgtatgacgg ttaatgaagt ggttacagca ttagaatcag gggttgatat
    10081 ggtcaaaatt tttcctggtt ctactgtagg gatgtccttt attcgggcaa tcaaatctcc
    10141 tttgccacaa gtagaggtta tggttacagg cggagtttct ttagataatc tcaaagactg
    10201 gctagctgct ggagtagatg ttttaggcat aggaggcgag tttaatcagt tagcgagcca
    10261 aaaacaatat aacctaatca ctaagaaagc cgctcattat atcaaaagtt tgcgacaata
    10321 atctaaaaaa cgtttttacc ttggctttag tgctagtaaa aacgcttttt tatcattcat
    10381 tcaattaata tgcttctgct tatgtttttc cagatagtat agatataaga gcagctaatt
    10441 taacaagatg agcctgtcgt ttgatatata ggatataaac tttttatgat caaacaaaag
    10501 taataccttt tgaattcgtt agtattgacc tgatctaaag tgatatcact tagaatgaac
    10561 tagctgtaca gcttttacag caacttatgc taaaatagaa aaaaaggaga aatgttgatg
    10621 attaaaggaa ttatttttga tatggacggt gttttatttg atacagaacc tttttatctg
    10681 aggcgacgag aagatttttt taagacaaag ggaattccca ttgatcattt gaactctaaa
    10741 gattttattg ggggcaatct ccaagaatta tggaaagagt tgttaggtaa aaatagggat
    10801 gatgctatcg ttaaggcaat tacagctgac tatgacgctt acaaacaagc gcataagcct
    10861 ccttatcaaa aactgttgat tacagaagtg aactcttgtc ttgaacagtt gaaaaaacaa
    10921 ggtattaaac tggctgtggc atcaaactcg aagcgtcagg atgttttgtt ggcgttggag
    10981 acaacgcaaa taaaagatta ttttgaaata atcctagcgc gtgaagatgt ttctagaggc
    11041 aaaccttatc cagatatcta taataaagca gtacaaaaac taggattaca aaaaaaacaa
    11101 ctgcttgtag tagaagacag ccaaaaaggc attgctgccg ccaaagcagc caatctgaca
    11161 gtttttgcca ttaccgacta ccgatatggc attgatcaga gtcaagctga tcacaagata
    11221 gatcatttag gacaactgtg tgtaaaaatc ggttgttttg gatcgtgaag taacgcaact
    11281 aactgatcat tgatgatact tcaagacact taagaatacg tctaggactt tctacagacc
    11341 aataacaaga atacgtttat gtgtttttac tgttttttta gctacctatt tacaatatga
    11401 cactgaaaag agtactatgt ttaagttatt atcaaaaaaa ccgtcgcata agatcaccta
    11461 gaaaaggatt atgatttttg tgatttggat gcgatcgtta gtcgttgacc atctttatgt
    11521 ggcaggagat atgtttaacc gtggtcatta tttggatttg tttttagatt ggctagtgag
    11581 taaatataga aggatcataa tactgctttg acaaaggcag tatctgagtc catgagttgc
    11641 acggataatg tgactcggtt ttggttagct ataataatat ttggaagtgt tatgctgctt
    11701 gtcttcgtag gtccattgat tatagtcaga cttactttag agctgagctg actgtctaga
    11761 caagacgtga tggagatgtg atttctgacc aagtagaggt aaccagaaaa gggactttta
    11821 cgatgtaatt gaatttactt gttgcattct tgtgcatgtt aagagagaaa acccagataa
    11881 atcaggaagt aaaatggtta tgtttgatta gaattgtcct tctttttcct tgaagtctta
    11941 acttattttg attatgatag tagtatgact attaaggcag aaattaaggc actcgccaaa
    12001 gaaattggta tctctaaaat tggttttacg acggcagata actttgatta tttggaaaag
    12061 tcattgcgtg cttctgtgga agaggggcgc aattcaggat ttgaacataa agtcatagag
    12121 gatcgtatct cccctgaaag gcttttagaa tcggctaaaa cgattatttc aattggcgtt
    12181 gcttatcctc acaaacttcc tcagcaacct caaaaaacac cttataaacg tggcaaaatt
    12241 acacctagtt cgtgggggtt tgattatcat tatgtggtcg gcgaaaagtt agacagacta
    12301 tcaaaaggca ttgaagagct gtgtcgagat tttcctttgc aacaaaaagc aatggtcgat
    12361 actggagctt tagttgatac tgcagttgcc caaagagcgg gtattggttt tataggaaaa
    12421 aatggtctag ttatttctaa agaatatggc tcttatatgt ttttgggaga attaattacc
    12481 aatttagaga tcgagccaga caagcctgtc gattatgatt gtggtgactg tcgtcgttgt
    12541 ctagatgcct gtccaacttc ttgtttaatt ggtgatggct caatgaatgc caagcgttgt
    12601 ctatcatttc aaacccaaga taagggaatg atggacatag agtttcgaaa aaagattaaa
    12661 acagttatct atggttgtga tatttgccaa atttgctgtc cttataacaa aggtataaat
    12721 aatccaccag ctacagagat tgatcctgag ttggctcaac cagaattaat accatttttg
    12781 agtttatcaa atggacaatt taaagaaaaa tttggtatga ttgctggaag ttggcgaggt
    12841 aaaaatattt tacagcgtaa tgccattatt gcgctagcaa atgctcatga taaaaccgca
    12901 gtggtcaaat taatagagat tattgataaa aataataacc ctattcatac tgcaacagct
    12961 atatgggcat tgggagaaat tgtcaaaaaa ccaaacgatg agatactggc gtttatgagt
    13021 catttaacct taaaagatga agatagccaa aaagagttgg aattaatccg tcataaatgg
    13081 caattttaag ccttttatgg tatttttaag aaccttatga taaaataaaa cagattaaca
    13141 aagattggag aaatctcatg gaagtggctg aaattcgcca aaaaatagta gaaaataaag
    13201 agaagttgac tagcttcagg aggtctcttt gacttagatc gtttggaaga ggagattgct
    13261 cttcttgaaa atcacatgac tgagccagac ttttggaatg acaatattgc agcgcaaaag
    13321 acatctcaag aactaaatga attaaaaggt aagtatgata ctttccataa tatgcaagaa
    13381 ttatctgacg aaacagaact cttacttgag atgcttgacg aagatgattc tttgaaagaa
    13441 gaattagaag aaaatctaat gcagctagat aagataatgg gtgcttatga aatgacgctt
    13501 ctcttatctg aaccctatga ccacaataat gctatcttag aaatccatcc tggttcagga
    13561 ggaactgaag cgcaagattg gggagattta ctcttacgaa tgtatacccg ttttggcaat
    13621 gctaatggtt tcaaagtaga agtattggat tatcaagcag gggatgaagc tggtattaaa
    13681 tcggttaccc tctcttttga aggtcccaat gcttatggtt tgttgaaatc agaaatggga
    13741 gttcatcgcc tcgttcgtat atcacctttt gattcggcta aacgtcgtca tacctcattt
    13801 gcttcagttg aagtgatgcc agagttagat gatacgattg aagtagaagt acgtgatgat
    13861 gacattaaaa tggatacttt ccgttccgga ggagctggtg gtcagaatgt caataaagta
    13921 tcaacaggtg ttcgtttgac gcatattcca actggaattg tggtatcgtc taccgttgac
    13981 cgtactcaat atggaaaccg cgatcgcgct atgaagatgt tgcaggctaa gctatatcag
    14041 ttagaacaag aaaagaaagc acaagaagta gatgctctca agggtgataa aaaagaaatc
    14101 acttggggaa gtcagattcg ttcttatgtt ttcactcctt atacgatggt gaaggatcat
    14161 cgtactaact ttgagcttgc tcaggtagat aaggttatga atggagaaat caatggcttt
    14221 attgatgcct accttaagtg gcgcattgaa gactaaaaat aataaaggag aaacatggca
    14281 ctaatagaaa tgtctggtgt tactaaaaaa taccgccgct caacgaccgc cctcagagac
    14341 gtcaatgttt ctgttaacca aggtgagttt gtttatctcg taggcccatc aggtgctggt
    14401 aaatcaactt ttatcaaact cctttaccgt gaagaacaac tgacaactgg taagctatat
    14461 gtgggtgaat tcaatttaac taaactaaaa gcacgtgatg ttccgattct tcgtcgtcac
    14521 atcggagtag tttttcaaga ttacaaactg ctaccaagaa aaacagtctt tgaaaatgta
    14581 gcttacgcca tggaagttat tggtgaaaag cgccgccaca ttaaaaaacg cgtgccagag
    14641 gtactcgatt tagttggctt gaaacataag atgcgttctt ttccaagtca gctatcagga
    14701 ggggagcagc aacgtgtggc cattgcgcgt gcgattgtta acaaccctaa attattgatt
    14761 gcagatgaac cgacagggaa tttagatcct gagatttcat gggaaattat gcagttactt
    14821 gaacgtatta atgttcaggg aacaactatc ttaatggcga cacacaacag tcatattgtg
    14881 aatacattcc gtcatcgtgt tgttgctatt gaagatggtc gcatagtacg tgatgaagaa
    14941 aaaggagatt atggttacga tgattagata ctttttccgt catatttggg aatccattaa
    15001 aaatcttaaa cgcaactttt ggatgacatt tgcttcagtt agtatggtgg ctgttacatt
    15061 gactttagtt ggtgtttttg cggcgacatt gttgaatata cagcgtgttg cttcgggtgt
    15121 tgaaaataat gtccatatca atacctactt acaagttgat tcaactgatg ctgcaaaagt
    15181 tattcaaaat actgccggtg aacctgttaa taacgataac taccacagtg tttacgacaa
    15241 aattgctcag ataaaaggtg tcaaaaagat tacattttct agcaaggatg aacaattgaa
    15301 aaaattgcaa gaaaccttag gtgatgtttg gaatatgtat gaccaagaca ctaatccgct
    15361 ccaagatatc tatcttattg agacccagac gcctaaacaa gttaaggcga tcactaaaaa
    15421 gatcagaacg attgaaggcg tagaagccgc agattatgga gggattaact ccgataaact
    15481 atttaagttt tcaacattaa ttcaaacttg ggggctgatc ggaacagcaa tgctattatt
    15541 tgtagctgtc tttctcattt caaacactat tcgtatgact attatgtctc gtaaacgcga
    15601 cattgaaatt atgcggttag ttggtgctaa aaattcttat attcgagggc ctttcttttt
    15661 tgaaggggct tgggttggct tgttaggagc tgttttacca tctcttctga tttattatgg
    15721 atatgatctt gtttataagc attttgcaca agaattacaa cgtaataatc tctcgatgta
    15781 tccgcttgat ccttatgtat attacctgat tggtgctttg tttgtcatag gtattatgat
    15841 tggttcgctt ggttcagtgc tttctatgag acgttattta aaattctaaa aaagcgctgc
    15901 tgacgaaacg tagcgaccct caaaagttgg acctcttatg ttattccaag gacttcgttt
    15961 tgtattgcac aggactggtc cttttcattt tgttttgatt catttgttta tttgtaatag
    16021 gcaatatagt ttaaaatagc agtttctaag gtttcaagag aagaaaagca ccccaaaagt
    16081 tagatgtttg atgtatctaa cttttggggt gtcattcaaa atttagtggc tcttttttag
    16141 taaaatacta tgatttcagt tttaggataa cctcaaacag tatctaaaat agctatgtca
    16201 atgaaaaaag gggttagcat ttttttcgtg acaaatggta gtagaaggac catgcccagg
    16261 ataaactcgg taatgattag gtaaggtgaa tagttcttgg cgaataccag taatgaggtc
    16321 ctcaaagtta ctggtaggta agtcagtacg accgatagtt tcacgaaata gagcatcgcc
    16381 agtaacgact aattcatctg agtggaaaac aaatgagact cctccccaag aatgaccagg
    16441 tgttggtaaa actgtgaatt caaaaccatt tagttgatag ggttgcttta atttaaaaaa
    16501 gttctctgcc ggtcttgcta taacagtaat gatatcatca tgacggccta agccagataa
    16561 attatcatct ggggaagata gccaagcggc ttctttttct gaaacataaa caggtggatg
    16621 atcaaaagca tctctgacca aatctaaact aaaaatatga tcataatgag tatgcgttaa
    16681 gaggatagcc actaaaggtt tttcaaagga tttaatttta gcaatgattt cttggccgtt
    16741 acttcctgga tcaatgagga taacagcttg atcattaacg aggtaataag tattttcacc
    16801 agcaatgtgg tttattgttt tatagatttt cataaggata gtgtaccaaa aaatcaaggc
    16861 tttgttaaaa aaagagtggc ggaaaataaa tggcaattag tttcagaaaa tatgagatct
    16921 cacagcaatt tcttgacacc gttttcataa aggtgtatca taaaagtaat caataagcca
    16981 agcctaaagc tgaacttagt ttaagaacac tgactttttg agagcaacct aatcggttga
    17041 ctaaaaaata tgaggtggaa gatatgtcaa aagttgctat tgttactggt gctggtcaag
    17101 gaattggatt tgctattgcg aaacgtcttc atgcagacgg ctttaaaata ggcattttag
    17161 actataatga agagactgct caggctgcgg ttgataaact atcacctgag gatactgttg
    17221 cagttgtagc agatgtttca aaacgtgacc aagtttttga tgctttccaa aaagttgtag
    17281 atacttttgg tgatttgaat gttgttgtta acaatgcagg tgttgcccca acaacccctc
    17341 ttgacacgat tactgaagag cagtttgaaa aggcttttgc aatcaatgtc ggaggaacga
    17401 tttggggaag ccaagcagct caaaaacatt ttagagaatt aggtcatggc ggaaaaatta
    17461 taaatgcaac atcacaagca ggatgtgaag ggaatcctaa tttgactgtt tatggtggta
    17521 caaaatttgc agttcgtggt attacacaga ctttggcaaa ggatttggca tcagaaggaa
    17581 tcacagtcaa tgcttacgca ccaggtattg ttaaaacacc tatgatgttt gatattgccc
    17641 atgaagttgg taaaaatgca ggcaaagatg atgaatgggg tatggagcaa tttgcaaaag
    17701 atatcacttt gaaacgcttg tctgaaccag aagatgttgc taatgcagta ggcttcttag
    17761 cgggagatga ttcaaattat atcactggac aaacaatagt tgtcgatggt ggtatggtat
    17821 tccattaaaa atgattaata ctgacactgt gagtttcact cacagtgttt tttaagaagt
    17881 ataaaaaatt atcctatctt ggttattgga gtgatggcac ctttgagata tagtcatgtt
    17941 ttgttttata gatatagcgt gctataatag acttactatg acccagaaaa aacttagaaa
    18001 atatgcagta gtggacttgg aagctactgg agcaggtccc aatgcttcta ttattcaggt
    18061 tgggattgtt attatccaag gaaataaaat tattgatagt tatgaaacag atgttaatcc
    18121 tcatgaatct ttagatgagc atattgttca tttgacaggt attacggata agcagttggc
    18181 taaagctccg gattttggtc aagtggcgca tcacatttat caattgattg aagattgtat
    18241 ttttgttgcc cacaatgtaa agttcgatgc aaatttattg gcagaagctc tttttttgga
    18301 aggatatgag ttaacaatac cacgtgttga tactgttgaa ttagcccaac tatttttccc
    18361 tcgctttgaa aagtataacc ttggtcacct atctaggcag ctcaatattg atttggcaga
    18421 agctcatact gctattgcag atgcaagagc tacagctata ctgtttttaa gacttttaca
    18481 aaaaattgag agccttccaa ttgagtgctt agaaagctta ttagtctatt cagatagcct
    18541 gctatttgaa acagcaatgg tcattcaaga aggattagcc aaagccaaac cttatgatcc
    18601 caacaaatac attaaaatca gacagattct cttaccaaag ggcccaaaag ctctcaagcc
    18661 ttatcaaata tcaaaaagct tccctattaa tatggcacta ttaggtctgg aagaaaggcc
    18721 taaacagact caatttgctc agttaattga cgaagattac catcagggag tagctagttt
    18781 tattgaagct cagacaggta ttggaaaaac ctacggctac ctgctacctc ttttggcaaa
    18841 agaagatcaa aaccaaatca ttgtcagtgt gccaaccaaa cttcttcaag accaattaat
    18901 ggctggtgaa gtagctgcta ttcaagaaca atttcacatc gcctgccata gtttaaaagg
    18961 acccgccaat tacctaaagc tagatagctt tgcagatagt ttggaccaaa atgaccagaa
    19021 ccgtttggtg aatcgttata aaatgcaatt attggtttgg ctcttagaaa ctaagacagg
    19081 agaccttgac gaaatcaaac aaaagcagcg ctttgccgct tattttgagc agcttaagca
    19141 cgatggagac attaagcagt cttctgaatt ttacaattat gatttttgga gagttaatta
    19201 tgaaaaagcc aaaacagccc gtctcctgat taccaaccat gcttattttt tacaccgtgt
    19261 tcaagatgac aaggactttg ctagaaacaa ggtcttggtc tttgatgagg cccaaaagct
    19321 gatgctccaa ttagatcaat tatcacgtca tcagcttaac ctgacggttt ttttacaaac
    19381 aatacaggcc aagttaagca accctttacc tcttttagaa aaacgcttat tggaaagctt
    19441 gtcgtttgaa ctgggacaag tgtcaagtga ttactatcaa aataaagagc accagcttgc
    19501 tcatgattgg tctcgtatag ctggctacgc caaagaatta acaggagcag attatcaaga
    19561 gttacaagcc ttttttgcta cctcagatgg tgactactgg cttagtagcg aaaagcaaga
    19621 ggagaagcgg ttgacttatc taaacagtgc tagtaaggca tttattcatt tccaacagct
    19681 cttacccgaa acggtgaaaa cttattttgt ttctgccact ttaaccatca gctcagaagt
    19741 aaccttggca gacctattag gttttgaaga gtatctctat catgtcattg aaaaggataa
    19801 aaagcaagac cagttggtgc ttgttgatca agaggcgcct atcgtgacag aagtcagtga
    19861 ccaaatctat gtggaagcaa ttgcaaaacg aatcgaaagc ttgaagcaag agggttatcc
    19921 tattttagtg ctttttaatt ctaaaaaaca cttgctgttg gtttcagact atctagacca
    19981 atggcaggtg cctcacttag cgcaggaaaa gaatggaaca gcttataata ttaaaaaacg
    20041 gtttgatcaa ggagagcaaa ctattttgct tggtttaggt tctttttggg aaggagttga
    20101 ttttattcaa gcggatcgta tgattaccct tatcgcacga ttgccctttg ataacccaga
    20161 ggatttcttt gttaagaaaa tgagtcatta tctattggaa aaaggtaaaa atcctttcag
    20221 agattacttc ttgccaatga ctattttgcg attaaaacag gctattggtc gcacgatgcg
    20281 tcgacaagat caaaagtcag tggttattat tttggatcgt cgtctcctta caaagtccta
    20341 tggacaagtt attttggaag gtctcggtca ggaatttttg atttctcaac aaaattttca
    20401 cgattgtcta gtagaaacgg actgtttttt gatataatag aaaggatcta ttatatcaga
    20461 aaaattttta atggcgttaa gcacaatcaa ctaacattta aattagtttg aacttttatt
    20521 atgctctttt agacaactgt tcacatgttt tcgaattgtt tttttgataa ggacaataaa
    20581 tgaacaaatt tttggtttta gtaaacccaa ttactgttct tttcattagt gtcagttgct
    20641 atcaaaacag cttgtcatag ttcgtgcgat cagtagcgag tattggtaaa actgaaaccg
    20701 tagattatga aaaaatgatc tgttgatcat taattaatct gaagataaga agtcgataaa
    20761 aaggagatac gatgcccaaa ctatcaaagc gagttttaga aatgaaagag agtgttactc
    20821 tggctgcagg tgctagagct aaggctttga aagcgcaggg ccgcgatgta ctgaatttaa
    20881 ctttaggaga accagatttt tttacaccta agcacattca agacaaggcg attgaatcta
    20941 ttcaaaatgg tacggctagc ttttatacaa atgcttctgg gcttccagaa ttaaaagctg
    21001 cgattgccac atacctgaaa aatcagtatg ggtatcactt gtctcctgat cagattgtgg
    21061 caggaacagg tgccaagttc atcctttatg ccttttttat ggctgtttta aatcctggtg
    21121 accaagttct cattccaaca ccatattggg tatcttattc tgaccaagta aaaatggcag
    21181 aaggtcagcc gatcttcgtt caaggactag aagaaaatca attcaaggta actgttgacc
    21241 agctggaaag agcaaggaca agtaagacta aagttgtcct tatcaattcc ccttccaatc
    21301 caacaggtat gatttatggt gctgaggaat taagggccat tggagaatgg gcggtgcaca
    21361 acgatattct tattctggca gatgatattt acgggtcctt agtctataac ggcaatcagt
    21421 ttgttcctat ttcaacactc tcagaggcga ttcgtcgtca aaccattacg gtcaatggcg
    21481 tggctaaaag ttatgccatg acaggctggc gggttggttt tgcagcaggt gaaccagaga
    21541 tcatttcggc aatgagtaag atcatcgggc agactacctc taacctgaca accgtttcac
    21601 aatatgctgc tatcgaggct ttctgtggta gtcagtcatc acttgaagag atgcgattag
    21661 catttgaaga acgccttaat atcacttatc cattgttgtg tcaggtccct gggtttgagg
    21721 ttgttaaacc acaaggagct ttttacttct ttccaaacgt taagaaagcc atggaaatga
    21781 caggtttttc cgatgtgaca agctttgcta atgctatttt agaggaagtc ggattagctg
    21841 ttgtgagtgg agcaggcttt ggcgcaccgg aaaatgttcg tctgagttat gctacagata
    21901 tcgaaaccct taaagaagct gttcgtagac tgcacgtctt tatggaaagt aatgaaatta
    21961 attaaataaa aatagagaga aacttatgtc aaaaaaactt atttcaattg ttgatgttaa
    22021 agattatgtt ggtcaagaag ttactattgg tgcttgggtt gctaacaaat caggtaaagg
    22081 taagattgcg tttgtacagt tacgcgatgg ttctgctttt ttccaaggtg tcgcttttaa
    22141 accaaacttc attgaaaaat atggtgaaga atcaggtctt gaaaaatttg acgttattaa
    22201 acgtctcaac caagaaacat ctgtttatgt aacagggatt gttaaagagg atgagcgttc
    22261 taaatttggc tatgagcttg atattactga ccttgaaatc attggagagt cacatgaata
    22321 tcctataact cctaaagagc atgggacaga cttcttgatg gacaaccgtc atctttggtt
    22381 gcgttctcgt aaacaaatgg ctgttatgca aattcgtaac gctattattt atgcaactta
    22441 tgaattcttt gaccaaaatg gttttatcaa atttgatagc ccaattttat cagaaaatgc
    22501 agcagaagat tcaacagagt tattcgaaac agactatttt gggaaaccag catttcttag
    22561 ccaatctggt caattatacc ttgaagctgg agcaatggct ctaggccgag ttttcgactt
    22621 tggtccagtc tttcgtgctg aaaaatctaa aacacgtcgc catttaacag aattttggat
    22681 gatggatgct gagtattctt tcttatcaca tgaggaatca cttgaccttc aagaagctta
    22741 tgttaaagca ttgatccaag gcgttctaga tcgtgcgcct caagcacttg atattttaga
    22801 acgtgatgtt gaggctctca aacgttatat cacagagcca ttcaagcgtg tttcttatga
    22861 tgatgctatt acacttcttc aagagcatga agcagatgaa gatacagatt atgaacattt
    22921 agagcatggt gatgactttg gttccccaca tgaaacttgg atttctaatt actttggagt
    22981 accgacattt gtagtgaact acccagcaag ctttaaagca ttttatatga aaccagttcc
    23041 tggaaatcca gaacgcgttc tttgtgctga tcttttagct ccagaaggtt atggagagat
    23101 cattggcggc tcaatgcgtg aagacaacta cgatgctctt gtggctaaaa tggatgaact
    23161 aggtatggat aaatcagaat atgactttta ccttgacctt cgtaaatatg gctctgttcc
    23221 tcatggcggt tttggtatag gtattgaacg tatggtaact tttgttgctg gtaccaaaca
    23281 tattcgcgaa gctattccat tcccacgtat gttacatcgg attagaccct aataatacaa
    23341 aaaagttcat gtcatctatg aactttttgt attggtaaga agtgagtgtg ttataaaaac
    23401 atcaatatgt cctaaaggag agataagaaa tagtagagtt tgaacctaca tcctttgtga
    23461 agataaattt tggaagttta gtagatcagt tagcagataa gacaatatag ccttgctcaa
    23521 gggatatttt atagacaagt atttggacta cgattatcaa atagacactc tcatttgata
    23581 ctaaaaaggt ctcgtctatt cctataatag gagagctacg ggagttatta ttaaaattag
    23641 ctgattttta cagttcaaaa tgtgttacca ctttttcact tcttagcttt cttatgtatg
    23701 tggtataatg aaggtggacg gttcgataga gaaagggaaa ttgtaaatgt cagacaaaca
    23761 cattaattta gttattgtga caggaatgag cggcgctgga aaaacagttg ccattcagtc
    23821 ttttgaggat ctaggctact ttaccattga taatatgccc ccagccttgg ttccaaaatt
    23881 tttagaatta attgaacaaa ccaatgaaaa tcgtagggtg gctttggttg tcgatatgag
    23941 aagtcgtttg tttttcaagg aaattaattc taccttagat agtattgaaa gcaatcctag
    24001 cattgatttt cggattcttt ttttggatgc aacggatgga gaattggtgt cacgctataa
    24061 agaaaccaga cggagccacc ctttggctgc ggacggtcgt gtgcttgatg gtattcgatt
    24121 ggaaagagaa ctcctatctc ctttgaaaag catgagccaa catgtggtgg atacaacaaa
    24181 attgacccct agacaattgc gtaaaaccat ttcagaccag ttttctgaag ggtctaatca
    24241 agcctctttc cgtattgaag tgatgagctt tgggttcaaa tatggtcttc ctttggatgc
    24301 ggatttggtt tttgatgtgc gttttctacc caatccttat tataaggtag agcttcgtga
    24361 aaaaacagga ctagatgagg acgtttttaa ttatgtgatg tctcacccag aatcagaggt
    24421 gttttacaag catttgttaa accttattgt ccctatctta ccggcttacc aaaaagaagg
    24481 gaagtctgtc ttgacggtgg ctatcggctg cacaggaggc caacaccgca gcgttgcctt
    24541 tgcccattgc ttggcagaaa gtctggcaac agattggtcg gttaatgaaa gccatcgtga
    24601 tcaaaatcgt cgtaaggaaa cggtgaatcg ttcatgaaaa atcctaaaat gactgttata
    24661 ggtgggggta ctggcatctc cattatccta aaaagccttc gtaatgaagc agttgatatt
    24721 actgctgtgg tcactgttgc agatgatggt ggttcctcag gagaacttcg taatgccatg
    24781 caattagccc cgccaggaga cttacggaat gtcttgttag cgatgagtga catgccaaaa
    24841 ttttatgagc gggttttcca ataccgtttt aacgagaatg atggagcctt ggctggtcac
    24901 cctctaggca atctcattat tgctggtatc tctgaaatgc aaggctcaac ctacaatgcc
    24961 attcaaattt taaccaaatt tttccatatt acaggtaaaa tttacccctc tagcgagcag
    25021 gccttgaccc ttcatgctgt ttttaaagat gggcatgaag tagcaggaga aagttctatt
    25081 gccaaatacc aaggcatgat tgaccatgtc tatgtgacca acacctacaa cgaccaaaag
    25141 cctcaggcca gtcgtaaggt ggttgaggct attctagaaa gtgacatgat tgtcttggga
    25201 ccaggctcac tgtttacctc tattttacct aatttggtca ttccagaaat taaagaagct
    25261 ttgcgccaga ctaaagcaga agtggtgtat atttgtaata tcatgaccca gtatggtgaa
    25321 acagaacaat tttcagatgc agaccatgtg gctgttttaa atcagcactt aggaagagat
    25381 ttgattgata cggttttggt aaatgtggct aaggtgccgc aggcttacat gaattctaac
    25441 aaatttgatg aatacctcgt tcaggttgat cacgactttg ctggcctttg cagggctgct
    25501 aagcgtgtga tttcgtctta ttttttacgg ttggagaatg gcggagcttt tcacgatggt
    25561 aacttagtgg tggaagaatt aatgaattta gtgaggatag tcaagcaatg agttttacaa
    25621 ccaaggtcaa agaggaatta attcacttgt ccacaggtga caacaatgaa ttggccgcca
    25681 ttatcaaatt atcaggcagc ttgggcttag ctcaccaaag ccttcattta tccattacta
    25741 ctgaaaatgc taaaattgcc cgctacattt attctcttat tgaagacgcc tatgttatcg
    25801 tgccagaaat caggtatcat caaaagacga acctgcgcaa aaaccgtgtt tacaccgtct
    25861 atgttgagca aggtgtggaa accattttag ccgatttgaa attagcggac tccttttttg
    25921 gtctggaaac aggcatcgaa cctcaggttt tatcagacga taatgctggc cgttcttatc
    25981 ttaaaggggc ttttctagcg gcaggtagca tcagagatcc tgagtcaggc aagtatcagt
    26041 tagaaatcta ttcagtttat cttgatcatg ctcaagactt agctcaactc atgcaaaaat
    26101 ttatgctaga tgccaaaacc attgagcata aaagcggtgc agttacttat ttgcaaaagg
    26161 cagaagatat catggacttt ttaattataa ttggtgccat gtcctgcaaa gaagactttg
    26221 aagccatcaa attacttcga gaagctcgaa atgatattaa ccgtgccaat aatgcagaaa
    26281 cagctaatat tgccaaaact atcagtgcta gcatgaaaac cattaataat attattaaaa
    26341 tcatggacac aattggttta gaaagtttac cgattgaatt gcaacaggta gcccagcttc
    26401 gggttaagca tccagactat tccattcaac aagttgctga tgcccttgag ttcccaatca
    26461 ccaagagcgg ggtcaaccat cgcttgcgta aaatcaataa aattgcagat gacttataga
    26521 acatttttat cgtatgagcc tttgttcata cgatttttct gtctaaaagt cgttttccat
    26581 tgtttttttg aagtgtctga tgttctgtga tatgataaaa gggataataa cgtttgtaaa
    26641 ggagactgat aatggcatgt acaactatac tcgtcggtaa aaaggcatct tatgatggct
    26701 caaccatggt tgctcgaaca gaagattctc aaaatggaga tttcacgcct aaaaaaatga
    26761 ttgtggtgaa accagaagat caaccgagac attaccgttc ggtgcaatct tcctttgaga
    26821 tggacttacc ggacaatccc atgacttaca cgtctgttcc cgatgcgctt ggtaaagatg
    26881 gtatctgggc agaagcaggc gtgaacgagg ctaatgtggc catgtcagca actgaaacca
    26941 tcactaccaa ttctcgtgtt ttaggggcag atcctctagt agcttctggt attggtgagg
    27001 aggacatggt gaccttagtg ctcccttata tccgttctgc gcgtgaaggg gttttgcgcc
    27061 ttggtgccat tttagaagac tatggcactt acgagtcaaa tggtgtggct ttttcagatg
    27121 aacatgacat ttggtggctg gaaacgattg gtggtcatca ctggattgca agacgggtgc
    27181 cagatgatgc ttatgtcacc aatcccaatc aatttggcat tgatcatttt gaatttaaca
    27241 atcctgaaga ttacctttgc tcagctgatt taaaggactt tattgacacg tatcacttag
    27301 atttaaccta tagctatgag cattttaatc cgcgctatgc ctttggtagt caacgtgaca
    27361 aagaccgtca ctataatacg ccacgtgctt ggatcatgca aaaattctta aacccagaaa
    27421 ttgtgcaaga cccgtgcagc tttgcccttg cttggtgtca aaaaccttac cgtaaaataa
    27481 cagtagaaga tgtcaaatat gtattgagta gtcactatca ggacacggtt tatgaccctt
    27541 acggttcaga aggaactcct gttagtaaga aagtctttcg ccctattggc attaaccgta
    27601 ccagccaaac cgctatctta cacattcgtc ctaataaacc tcaagaaatt gcggctatcc
    27661 agtggatggc ttatgggtca atgccattta ataccatggt gccttttttc acacaggtga
    27721 agactattcc agattatttt gccaatactt acgaaaatgt gtctaccgac aatttctatt
    27781 ggaccaaccg tctgattgcg gctctagcag atcctcacta caatcatcat gaaactgatt
    27841 tggacaatta cttggaagaa accatggcaa aaggtcatgc catgttacat gctgtggaag
    27901 cacagttatt ggctggtgaa accgtcgatt tggaagagga aaatcaaaaa atgagtgact
    27961 atgtgcaagg agaaacgcag acgctgttga acaagattct ctttgacgct agcaatctaa
    28021 tgactaatcg tttcagtttg agcgattaaa agtgaagcta aggctgagca actgcttagc
    28081 cttttttacg ataacttttt aggaaaagta atcgcaaaaa gacaaaaact tggaaaaaaa
    28141 atcaaagtat gctatactta actggtcaat ttttaaccag ttaataacaa aggaggaatc
    28201 atgaaaaaga aaattctttt aatgatgagt ttaatcagtg tcttttttgc ttggcaactt
    28261 actcaggcaa aacaagtctt agcagagggt aaagtgaagg tggtgacaac tttctatcct
    28321 gtttatgaat ttacaaaagg ggttattggt aatgatggcg atgtttccat gcttatgaaa
    28381 gcaggaacgg aacctcatga ttttgagcct tctacaaaag acattaaaaa aatccaagat
    28441 gcagatgcat ttgtttatat ggatgacaat atggaaactt gggtttctga tgtgaaaaaa
    28501 tcattgacat ctaaaaaagt gaccatcgtc aagggaactg gtaacatgct cttggtagca
    28561 ggagctggac atgaccatca ccatgaggat gctgacaaaa agcatgagcg taataaacat
    28621 agcgaagaag gacacaacca tgcttttgac ccacacgtgt ggttgtcacc
//
LOCUS       DAUCLR010000015        28123 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.015, whole genome shotgun sequence.
ACCESSION   DAUCLR010000015 DAUCLR010000000
VERSION     DAUCLR010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28123
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.015"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(87..1802)
                     /locus_tag="VGT87_000513"
     CDS             complement(87..1802)
                     /locus_tag="VGT87_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ3813396.1"
                     /translation="MIKTDHHLLKRMLHDLLKKPLPVCILVIASFVQVGLSVYLPVLI
                     GKAVDMSLSVNSWQTLKWLLGQMLVVIVVNTLIQWVMPLVYSRLLYQYSQQLKDKLLE
                     KIHRLPFAYLDRQTIGDLVSRVITDTEQLINGLQMVFNQFILGLLTILCTIIAMAQID
                     WFMLILVLVLTPSSLFLARFIAQKSFHYAQAQTKSRGNLAQFTEEILRQEGLVQLFNA
                     QEQSICDYHVLNKTYCEASQKVIFYASTVNPATRFINSVIYALLAGLGAVRIMAGLFS
                     VGQLTTFLNVVVQYTKPFNDISSVLAEIQSSLACAQRLYDLLDIEIKEEEHFLTFKAS
                     AVKGQIDFEEVSFSYQKDRPLLKDINFSVPAGSKVAIVGPTGAGKSTLINLLMRFYEL
                     DAGSIKLDKVPIKCYAKEELRSITGMVLQETWLKDATVHELIAYGSEEASRGEVVAAA
                     KAAHVHFFIMQLPKTYDTYLSASDDALSQGQLQLLAIARMFLKKPKVLILDEATSSID
                     IRTEAVIQEALKERTSFIIAHRLSTIQSADLILVMDQGRLVEWGTHASLMSKNGCYAR
                     LQKIE"
     gene            complement(1799..3523)
                     /locus_tag="VGT87_000514"
     CDS             complement(1799..3523)
                     /locus_tag="VGT87_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ3813397.1"
                     /translation="MKRLRPYLKGYLKESILGPLFKLLEALFELLVPLLIANMIDISI
                     SQHNSQGILRVVLTLFGLATIGLLLSVTAQYFSSKAAVGFTRQMTDDLFKKIMFLSKE
                     DQDHLGYASLLSRLTSDSFQIQTGINQFLRLFLRAPIIVCGATVMAYWISPSLTLWFV
                     MMVIVLLTLVFVMSHLLGPLYLLIRRETDHLVRLTSQQLQGIRVIKAFNQTQKELQAF
                     KQQNMLLSRHQYQAATLANVLNPMTFLVVNLTLLILIWQGSWQVAHRSLSQGMLVALI
                     NYLLQILAELLKMTMLMGTINQSVTAAKRINQVFVLADEAPLPSLKDGPISTHLLTIR
                     HLTFTYPGAAEPSLYDIQLSADQGEWIGIIGGTGAGKTTLIDLICQTYSPYSGEISLN
                     WQGEVPKTLTEWRNVIALVPQKAQLFKGTIRSNLLLGQSMPISDEELWRALELAQAKE
                     FVAALPEQLEAPVEAFGRHFSGGQRQRLAIARALLKPKPILILDDASSALDNETRGRL
                     FKALKEELSDVLVILVTQSIKNLQFADKILVLEQGHQLDFASHDQLKVSNALYQEMLA
                     LYVKGEQK"
     gene            complement(3533..4132)
                     /locus_tag="VGT87_000515"
     CDS             complement(3533..4132)
                     /locus_tag="VGT87_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEQ3813398.1"
                     /translation="MFRLLKRACSFLLLFVIYQSFVIHHNVQRVLAYKPMVEKTLAEN
                     DTKANVDLVLAMIYTETKGGEADVMQSSESSSGQKNSITDSQASIEHGVNLLSHNLAL
                     AEEAGVDSWTAVQAYNFGTAYIDYIAKHGGQNTVDLATTYSKTVVAPSLGNTSGQTYF
                     YYHPLALISGGKLYKNGGNIYYSREVHFNLYLIELMSLF"
     gene            complement(4133..5110)
                     /locus_tag="VGT87_000516"
     CDS             complement(4133..5110)
                     /locus_tag="VGT87_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEQ3813399.1"
                     /translation="MLDSYIKRIVIHQFSPNDTELLLSDRLVSITPRIDEYFRKKLAK
                     VFSDEAKRGQFEANNTFFTTIGDDLLETSVTIAQLWKEAFVISEDQKTNDLVFVQFDK
                     DGEPFFAFLRIALKEQFAHLSDNYEHPFTVTQNNLPSPTQTPDEALVINLKSGQYYLI
                     EKRVKHNGSFANYFSEHLLKVTPEQSVKKSIKMIEQTAQKIAEHFNQDDFTFQSKMKS
                     TLFKQLEADDVLSPEKLADQLFDDNLTARLTFVDQVKEVIPEPIKISDIDHSRQIKKL
                     ENQKLSLSNGIELTVPNAIYQDAEAVEFLLNDDGTYSILIKNIEDIKTK"
     gene            complement(5117..6373)
                     /locus_tag="VGT87_000517"
     CDS             complement(5117..6373)
                     /locus_tag="VGT87_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEQ3813400.1"
                     /translation="MIFDKGNVEDFDKELWDAIHAEEERQEHHIELIASENMVSKAVM
                     AAQGSVLTNKYAEGYPGNRYYGGTECVDIVETLAIERAKKLFGAAFANVQAHSGSQAN
                     AAAYMALIEAGDTVLGMDLAAGGHLTHGSPVNFSGKTYHFVGYSVDADTEMLNYEAIL
                     EQAKAVQPKLIVAGASAYSRSIDFEKFRAIADHVGAYLMVDMAHIAGLVAAGVHPSPV
                     PYAHIVTSTTHKTLRGPRGGLILTNDEALAKKINSAVFPGLQGGPLEHVIAAKAVAFK
                     EALDPAFKDYAQAIIDNTAAMAAVFAQDDRFRLISGGTDNHVFLVDVTKVIANGKLAQ
                     NLLDEVNITLNKNAIPFETLSPFKTSGIRIGCAAITSRGMGVKESQTIARLIIKALVN
                     HDQETILEEVRQEVRQLTDAFPLYKK"
     gene            complement(6363..6815)
                     /locus_tag="VGT87_000518"
     CDS             complement(6363..6815)
                     /locus_tag="VGT87_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ3813401.1"
                     /translation="MLRPLSKTDCPMLQEINAKALGYLVSLDLLERQYERLIEDCHHY
                     FLAYADKDTNQLLGYVHAERYETLYASDGLNLLGLAVLPAYQRRGIGSALLRALESQA
                     RQEGIAFIRLNSASHRKEAHAFYRNLDYADDKTQLRFIKNLQGDDNDF"
     gene            complement(6833..7423)
                     /locus_tag="VGT87_000519"
     CDS             complement(6833..7423)
                     /locus_tag="VGT87_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEQ3813402.1"
                     /translation="MEYLASIIESGDALVLPTETVYGLFAKALDEKAVNAVYDLKQRP
                     RDKAMNLNVADFNSILAFSKEQPRYLKKLYQAFLPGPLTIILKANDQVPYWINSGLST
                     VGFRLPNHPITAALIQKTGPLIGPSANLSGKASGRVFDHIMQDFDFQVFGYADDPFLT
                     GKDSTILDLSGERAVILRQGAITKEELLANVPELRF"
     gene            complement(7407..8246)
                     /gene="prmC"
                     /locus_tag="VGT87_000520"
     CDS             complement(7407..8246)
                     /gene="prmC"
                     /locus_tag="VGT87_000520"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEQ3813403.1"
                     /translation="MNYATLIRTYEDKLEQIDEDRENLAYVFREIKEWSSLDMLIHQN
                     QAVTPEDAVLLEHIFCSLSQHLSPQYITGNAYFRDLKLAVDKRVLIPRPETEELVDMI
                     LAENLDAPLNVLDIGTGSGAIAISLKKERSNWQVTASDISRAALDLAKANADAYQLDI
                     TFIESDVFSLISGTFDIIVSNPPYISYEDKEEVSLNVLQSEPHLALFAKENGYAIYRK
                     IIEQADNYLAKEGKLYFEIGYKQAEGIKDMLQAYFPQRHIRVVTDIFGKERMVVVDYG
                     VSS"
     gene            complement(8246..9325)
                     /gene="prfA"
                     /locus_tag="VGT87_000521"
     CDS             complement(8246..9325)
                     /gene="prfA"
                     /locus_tag="VGT87_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEQ3813404.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSREEANTR
                     ETVTAYREYKQVIQTISDAEEMIKDASGDPELEEMAKEELKESKAAKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAETQGWRFEVMESSVNGVGG
                     IKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRIDIYHASGAGGQNVNKVATAVRMVHIPTGIKVEMQEERTQQKNRDKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTVGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKMDEVIDALVMYDQTKKLESLNN"
     gene            complement(9360..9929)
                     /locus_tag="VGT87_000522"
     CDS             complement(9360..9929)
                     /locus_tag="VGT87_000522"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEQ3813405.1"
                     /translation="MAQLYYKYGTMNSGKTIEILKVAHNYEEQGKPVVIMTSALDTRD
                     GFGIVSSRIGMRREAIPISNDMDIFTFIAQLEEKPYCVLIDESQFLSKQNVYDLARVV
                     DELNVPVMAFGLKNDFQNNLFEGSKHLLLLADKIDEIKTICQYCSKKATMVLRTENGK
                     PVYEGDQIQIGGNETYIPVCRKHYFNPEI"
     gene            10067..10252
                     /locus_tag="VGT87_000523"
     CDS             10067..10252
                     /locus_tag="VGT87_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEQ3813406.1"
                     /translation="MPFVTIDLFEGRSQEQKNQLAREVTEVVSRIAKAPKENIHVFIN
                     DMPEGTYYPQGEMKQKS"
     gene            10305..11243
                     /locus_tag="VGT87_000524"
     CDS             10305..11243
                     /locus_tag="VGT87_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEQ3813407.1"
                     /translation="MFVTQQLKLMGTVIDIQIESDKACQQLSRVIDLLYTYKNRFSAN
                     DSNSELMAINQAAGVKPVSVHSDLFNLIQIGKAYSLSTPSNLNIAIGPLVQAWRIGFE
                     DARVPSHNLISQQLALTDPRQVLIDDKKQTVFLQQVGMALDLGALAKGYITDKIMAYL
                     IEDGIDSALINLGGNVLVHGPNPKSPDKTFRIGIQKPDAKRGQHLGVIKVNNHSVVTS
                     GIYERQFTAKGKQYHHILDRQTGYPIETDMLSLTVMAPSSFYCDIWATRLFGLDSSMI
                     ITLLNTFDNIEGLLVTRKHHVLMSNGLRHYFQPYYH"
     gene            complement(11307..12590)
                     /locus_tag="VGT87_000525"
     CDS             complement(11307..12590)
                     /locus_tag="VGT87_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEQ3813408.1"
                     /translation="MTHSTKQEHSHSQSAVLGLQHVLSMYAGSILVPIMIAGALGYSA
                     RELTYLISTDIFMCGVATFLQLKLTKHTGVGLPVVLGCAFQSVAPLSIIGAQQGSSAM
                     FGALIASGIYVILVAGIFSKIARFFPPIVTGSVITVIGLSLVGVAMGNMGDNVKEPTA
                     QSMMLSLLTIVIILLVQKFTKGFVKSISILIGLVAGTLVSAMMGLVDTTPVVEASWIH
                     VPTPFYFGMPTFEITSIVMMCIIATVSMVESTGVYLALSDLTNDQLDEKRLRNGYRSE
                     GIAVFLGGLFNTFPYTGFSQNVGLVQISGIKTRRPIYYAAGILVVIGLLPKFGAMAQM
                     IPSPVLGGAMLVLFGMVALQGMQMLNRVDFQKNEYNFIIAAVSISAGLGFNGTNLFTS
                     LPETAQMFLTNGIVIATLTSVVLNLVLNGKDKQDE"
     gene            complement(12590..13171)
                     /locus_tag="VGT87_000526"
     CDS             complement(12590..13171)
                     /locus_tag="VGT87_000526"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEQ3813409.1"
                     /translation="MQLLEERILTDGNILGENILKVDNFLTHQVDYRLMKAIGKVFAQ
                     KYAEAGITKVVTIEASGIAPAVYAAEAMDVPMIFAKKHKNITMTEGILTAEVYSFTKQ
                     VTSTVSIAGKFLSKEDKVLIIDDFLANGQAAKGLIEIIGQAGAQVVGVGIVIEKSFQD
                     GRRLIEDMGIEVTSLARIKNFENGNLNFLEADA"
     regulatory      complement(13258..13353)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(13476..14459)
                     /gene="guaC"
                     /locus_tag="VGT87_000527"
     CDS             complement(13476..14459)
                     /gene="guaC"
                     /locus_tag="VGT87_000527"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEQ3813410.1"
                     /translation="MFNDIPVFDYEDIQLIPNKCIITSRSQADTSVTLGKYQFKLPVI
                     PANMQTIIDETIAEQLAKEGYFYIMHRFDEDSRKPFIKRMHEQGLIASISVGVKAYEY
                     EFVTSLKEDTPEFITIDIAHGHANSVIDMIKHIKTELPETFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLSALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMVMIGSLFAGHIESPGKTVEVNGETFKEYYGSASAYQKGEHKNVEGKKIL
                     LPTKGHLSDTLTEMQQDLQSSISYAGGKDLDSLRHVDYVIVKNSIWNGDSI"
     gene            complement(14737..16263)
                     /locus_tag="VGT87_000528"
     CDS             complement(14737..16263)
                     /locus_tag="VGT87_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEQ3813411.1"
                     /translation="MPSLFVTFQNRFNEWLAALGEHLQISLLSLMIALLIGVPLAALL
                     SRSKRWSDIMLQVTGVFQTIPSLALLGLFIPLMGIGTLPAVTALVIYAIFPILQNTIT
                     GLNGIDPSLVEAGIAFGMTKWERLKTFEIPIAMPVIMSGVRTSAVMIIGTATLASLIG
                     AGGLGSFILLGIDRNNANLILIGAISSALLAIIFNSLLQYLEKASLRRIMISFGITLL
                     ALLASYTPMALSQFSKGKDTVVIAGKLGAEPDILINLYKELIEDQSDISVELKSNFGK
                     TSFLYEALKSGDIDIYPEFTGTITSSLLRDKPPLSNDPKQVYEDAKKGIAKQDKLTLL
                     KPFAYQNTYAVAMPEKLAKEYQIETISDLKAHADTLKAGFTLEFKDRADGYKGMQSQY
                     GLQLSVATMEPALRYQAIQSGDIQVTDAYSTDAEITKYHLKVLKDDKQLFPPYQGAPL
                     MKTSLLTKHPELKGILNQLAGKITEKEMQDMNYEVSVKGADANKVARDYLLKTGLIQK
                     "
     gene            complement(16256..16984)
                     /locus_tag="VGT87_000529"
     CDS             complement(16256..16984)
                     /locus_tag="VGT87_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ3813412.1"
                     /translation="MIRFNNVSKTFGQTKVLQEQTFQINDREFFVLVGPSGSGKTTLL
                     KMINCLIEPSSGDILLNNVPQKELDLREMRLSIGYVLQQIALFPNLTVAENIAIIPDM
                     KQWSAEEIRQKTEELLDKVGLPAKDYLDRYPSDLSGGEQQRIGIVRAIISHPKILLMD
                     EPFSALDPISRKQLQELMLSLHKEFDMTIVFVTHDIDEAIKLGDRVAILNEGEIVQLD
                     RPEMIKTHPANAFVVNLFGGDEHA"
     gene            17401..18090
                     /locus_tag="VGT87_000530"
     CDS             17401..18090
                     /locus_tag="VGT87_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HEQ3813413.1"
                     /translation="MKNAISILALLTPENQLEDTFIRELSHHFSHLFEVTILTSKANI
                     ESNQLSTFQGIFSFHEHDIDLPTLYFKTSQHGQGFLVTESVFDQATAVLSLSQYLTRF
                     YQKFDRHFLEYLPLQARLSDAKGNILVDNHAFNGSFLPTTDNEIEDWILAELRLSDNP
                     CKTFLLPSGSLDHIYMQHYQALKNPQGQLVGVLDTVQDIKPLLSQYLEETGQAIVGWS
                     DVTSGPSILDH"
     gene            complement(18283..19038)
                     /locus_tag="VGT87_000531"
     CDS             complement(18283..19038)
                     /locus_tag="VGT87_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEQ3813414.1"
                     /translation="MGNIALVTGASAGFGRAIVEHLIADGIKVIGASRRLEKLKALET
                     SFGSDNFYPLQMDVTDTKVIDDALTSLPNQWQDITILVNNAGLALGLDKAYEADFENW
                     MTMINTNIVGLIYLTRQLLPHMVSKDDGIIINLGSTAGTISYPGANIYGASKAFVKQF
                     SLNLRADLAGSHIRVTNIEPGLCEGTEFSKVRFNGDEARVKAIYQGAHAIQPQDIAST
                     VSWVIHQPPHVNVNRIELMPVSQSYGPQPVTRD"
     gene            complement(19165..20160)
                     /gene="pta"
                     /locus_tag="VGT87_000532"
     CDS             complement(19165..20160)
                     /gene="pta"
                     /locus_tag="VGT87_000532"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEQ3813415.1"
                     /translation="MSIRSLFGGLREKILGKNMKIVFPEGNDERVVRAAARLKFEGLL
                     EPIILGQSEEVRNLLTKLGFADQDYTIINPNEYADFDKMKEAFVDIRKGKATLEDADK
                     MLRDVNYFGVMLVKMGLADGMVSGAIHSTADTVRPALQIIKTKPGISRTSGVFLMNRE
                     NTSERYVFADCAINIDPTAQELAEIAVNTAETAKIFDIDPKIAMLSFSTKGSGKAPQV
                     DKVREATEIAKGLNPDLALDGELQFDAAFVPETAAIKAPDSAVAGQANTFIFPDLQSG
                     NIGYKIAQRLGMFDAIGPILQGLNKPVNDLSRGSSAEDIYKLAIITAAQAIESQG"
     gene            complement(20164..21069)
                     /locus_tag="VGT87_000533"
     CDS             complement(20164..21069)
                     /locus_tag="VGT87_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEQ3813416.1"
                     /translation="MRFEFVADKRIKVKTLLKSHDVSKGLLAKIKYKGGNILVNGIEQ
                     NAIYLLQVGDVVTIDIPNEEPFEKLEAIPFDLDIVHEDDHFLVINKPIGFASIPSAIH
                     SNTIANFIKAYYVDNHYLDQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQTRSIE
                     KRYFALVSGNGTLPDEGDIIAPIGRSKDSIITRAVDPMGKYAKTSYKVVARYSENVHL
                     VDIKLHTGRTHQIRVHFAHIGFPLLGDDLYGGRLDLGITRQALHCHYLNFKDPFTESD
                     CSYAIHLTDDFDSVIIGLQKKDRGY"
     gene            complement(21066..21902)
                     /locus_tag="VGT87_000534"
     CDS             complement(21066..21902)
                     /locus_tag="VGT87_000534"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEQ3813417.1"
                     /translation="MTQMNYTGKVKRVAIIANGKYQSKRVASKLFSVFKDDPDFYLSK
                     KNPDIVISIGGDGMLLSAFHMYEKELDKVRFVGIHTGHLGFYTDYRDFEVDKLIDNLR
                     KDKGEQISYPILKVAITLDDGRVVKARALNEATVKRIEKTMVADVIINHVKFESFRGD
                     GISVSTPTGSTAYNKSLGGAVLHPTIEALQLTEISSLNNRVFRTLGSSIIIPKKDKIE
                     LVPKRLGIYTISIDNKTYQLKNVTKVEYFIDDEKIHFVSSPSHTSFWERVKDAFIGEI
                     DS"
     gene            complement(21877..22548)
                     /locus_tag="VGT87_000535"
     CDS             complement(21877..22548)
                     /locus_tag="VGT87_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEQ3813418.1"
                     /translation="MTLDWEEFLDPYIQTVGELKIKLRGIRKQYRKQNRYSPIEFVTG
                     RVKSIESIKEKMILRGVIEENIAQDIQDIAGLRIMVQFVDDVEEVLALLRQRQDMTIV
                     YERDYIRNMKSSGYRSYHVVVEYPVDTIEGQKKVLAEIQIRTLAMNFWATIEHSLNYK
                     YGGDFPEEIKKRLEVTAKIALELDEEMRKIREDIREAQLLFDPVTRNLSDGVGNSDDT
                     DELYR"
     gene            22636..23214
                     /locus_tag="VGT87_000536"
     CDS             22636..23214
                     /locus_tag="VGT87_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEQ3813419.1"
                     /translation="MTNLEIEYKTLLTKNEYNRLLFQMKHVTPVTQTNYYIDTKAFDL
                     KANKMSLRIRTFVNSAELTLKVPEKVGNREYNVPLFLEQAKDMIKHGNLPESTALDII
                     ISKGIKPSALVTFGNLTTVRRETVIPIGKLALDYNLYANTKDYELELEVSDALQGKID
                     FDSFLSEHHITFKYAKSKVARCINTLKKFNDK"
     gene            23354..24334
                     /locus_tag="VGT87_000537"
     CDS             23354..24334
                     /locus_tag="VGT87_000537"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEQ3813420.1"
                     /translation="MTERYADKQIKLFSLTSNLPIAEKIAKAAGIPLGKMSSRQFSDG
                     EIMINIEETVRGDDIYIIQSTSFPVNDNLWELLIMIDACKRASANTVNIVLPYFGYSR
                     QDRVAKPREPITAKLVANMLTKAGIDRVVTLDLHAVQVQGFFDIPVDNLFTVPLFAER
                     YSKLGLSGSDVVVVSPKNSGIKRARSLAEYLDSPIAIIDYAQDDSEREQGYIIGDVSG
                     KKAILIDDILNTGKTFAEAAKILERSGATDTYAVASHGLFAGGAADVLETAPIKEIIV
                     TDSVKTKNRVPENVTYLSASDLIAEAIIRIHERRPLSPLFSYQPKGKNNA"
     gene            24331..25458
                     /locus_tag="VGT87_000538"
     CDS             24331..25458
                     /locus_tag="VGT87_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEQ3813421.1"
                     /translation="MTYFDNAATTPLSPNVIRAMTAAMQDNFGNPSSIHFYGRRANKI
                     LRECRQAIARNLGASEQQIIVTSGGTESNNMAIKGYALAHQAKGKHLITTTIEHHSVL
                     HTMAYLEERFGFEVTYLPCQNGQINLSDLKQALRDDTILVSIMYANNETGDLLPIKGI
                     GNLLKDHQAAFHVDAVQAVGKLKIIPSELGIDFLSASAHKFHGPKGCGFLYSNGQPID
                     PLLHGGDQEGKRRASTENMLGIIGMAQALTDAMTCLDQSTDHIISLRHHLISLLEGLP
                     YYINQGTHYLPHVLNIGFLGYQNTILLTQLDLAGIAVSTGSACTAGAVNPSHVLAAYY
                     GDDSSRLKESIRISFSDQNSIEDVNQLAQTLKNILGGTDGF"
     gene            25448..25795
                     /locus_tag="VGT87_000539"
     CDS             25448..25795
                     /locus_tag="VGT87_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEQ3813422.1"
                     /translation="MAFEKEIKLKDCKYLYTISPNIKKYTLRDTTFSQTKVGHYQLIR
                     LLETIPNSGDGFPLKITINKELTGFKLAITDQSGLRLVNIFKTKDNQILQDKFYFLMD
                     SLVEREIFSKTVV"
     gene            26047..26691
                     /locus_tag="VGT87_000540"
     CDS             26047..26691
                     /locus_tag="VGT87_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEQ3813423.1"
                     /translation="MVIDKSIPKATAKRLSLYYRIFKRFHADQVEKASSKQIADAMGI
                     DSATVRRDFSYFGELGRRGFGYDVTKLMNFFADLLNDHSTTNVILVGCGNIGRALLHY
                     RFHDRNKMQIAMGFDTDDNALVGTKTADNIPVHGISSVKERIANTDIETAILTVPSIH
                     AQEVTDQLIEAGIKGILSFAPVHLQVPKGVIVQSVDLTSELQTLLYFMNQNHLD"
     gene            26701..27396
                     /locus_tag="VGT87_000541"
     CDS             26701..27396
                     /locus_tag="VGT87_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEQ3813424.1"
                     /translation="MTKPIIGITANQRLNMALDNLPWSYAPTGFVQAVTQSGGLPLLL
                     PIGDEAAAKTYVSMVDKIILIGGQNVDPKYYQEGKAAFDDDFSPERDTFELAIIKEAI
                     TLKKPILGICRGTQLMNVALGGNLNQHIDSHWQEAPSDFLSHEMIIEPDSILYPIYGH
                     KTLINSFHRQSLKTVAKDLKVIARDPRDGTIEAVISTNDAIPFLGVQWHPELLQGVRD
                     EDLQLFRLFVNDF"
     gene            complement(27398..28078)
                     /gene="radC"
                     /locus_tag="VGT87_000542"
     CDS             complement(27398..28078)
                     /gene="radC"
                     /locus_tag="VGT87_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEQ3813425.1"
                     /translation="MYSIKCDDNKAMPRERLMRLGAESLSNQELLAILLRTGNKEKHV
                     LELSSYLLSHLDSLADFKKMSLQELQHLAGIGKVKAIEIKAMIELVSRILATDKTLTD
                     SVLTSVQVAEKMMAALGDKKQEHLVVLYLDNQNRIIEEKTIFIGTVRRSLAEPREILY
                     YACKNMATSLIVIHNHPSGNIEPSSNDYCFTEKIKRSCEDLGIICLDHIIVSYKDYYS
                     FREKSTLF"
BASE COUNT         8831 a         5747 c         4841 g         8704 t
ORIGIN      
        1 ttattttttt atataacagt ttaaagtttg ttaattaact aactttagga agagttaaaa
       61 agttcacctt tgttaaaaga cgaactttat tctatctttt gtaatctagc ataacatccg
      121 ttttttgaca ttaagctggc atgtgttccc cactcaacca atcgaccttg atccataaca
      181 agaatcaaat cagctgattg aatcgttgat aaacgatggg caatgataaa gctggtccgt
      241 tcttttagtg cctcttgaat aacagcttct gttctaatat caatagagga ggtggcttca
      301 tctagaatca agacttttgg ttttttcaaa aacattctgg caatagctaa taactggagc
      361 tgcccttggg acaaagcatc atcagaagca cttaagtaag tatcataagt cttaggaagt
      421 tgcataataa agaagtgtac atgagctgct ttggctgctg ccactacttc accacggcta
      481 gcctcttcac tgccgtaagc aatcaactca tgaacagtcg catctttcaa ccaggtttct
      541 tgcaatacca tgccagtaat ggacctaagt tcttccttag cataacactt aataggaact
      601 ttatctaact tgatgctacc agcatcaagt tcataaaagc gcatgagtaa attgattaaa
      661 gtcgatttgc ctgcacctgt aggccctaca atcgctacct tggaacccgc aggaacagaa
      721 aaattaatat ccttcagtaa gggcctgtct ttttgataag aaaaacttac ttcttcaaag
      781 tcaatttgcc ccttaaccgc tgatgcctta aatgttaaaa agtgctcttc ctcttttatt
      841 tcaatatcta aaaggtcata aagcctttgc gcacaagcta aagaactctg tatctctgcc
      901 aagacagatg aaatatcgtt gaaaggtttg gtatattgga caacaacatt taaaaaagtg
      961 gttagctgac ctactgaaaa aagtccagcc atgatacgaa cagcccctaa acctgccagt
     1021 aaagcataaa tgacactatt aataaagcga gtggctggat tgactgttga ggcgtaaaaa
     1081 atgacttttt gtgaagcctc gcaataggtt ttatttaaaa catgataatc acagatagac
     1141 tgctcttgag cattaaacag ttgaaccaaa ccttcttgac gaaggatttc ttcagtaaac
     1201 tgagctaaat ttcctctgct tttggtctgt gcttgtgcat aatgaaaact cttttgagca
     1261 ataaaacgag ctaaaaagag ggagcttggt gttaacacca aaaccaagat taacatgaac
     1321 cagtcaatct gtgccatagc tataatggta cacagaatcg tcaatagtcc aagtataaac
     1381 tggttaaaaa ccatttgtag accattgatc agttgctcag tgtcggttat cactcgacta
     1441 accaaatctc caatggtttg acgatctaga tatgcaaaag gaagacgatg aattttttcc
     1501 aaaagcttat ctttcaattg ctggctatat tgatatagca aacgactata gacaagaggc
     1561 atcacccatt gaatcaatgt gttgaccaca ataacaacta acatttgccc caacagccat
     1621 tttaaagttt gccaagaatt gactgacaaa ctcatatcaa cggctttacc gataagaaca
     1681 ggcaagtaaa cacttaaacc aacttgtaca aaactagcta tcacgagaat acaaacaggt
     1741 agaggttttt ttaacaaatc gtgaagcatt cgctttagga ggtgatgatc tgttttaatc
     1801 atttttgctc ccccttcacg taaagcgcta gcatttcttg ataaagtgca ttgcttactt
     1861 ttaactggtc atgactggca aaatccaatt gatggccctg ttccaacact aaaatcttat
     1921 cagcaaactg caaattcttg atggattgcg tgactagaat cactaaaaca tctgataact
     1981 cttcttttag agctttaaaa agtcgtcctc gagtctcatt gtctaaggcg gaactggcat
     2041 catccaaaat caaaatgggt tttggcttca gaagtgctct ggcaatagct agacgttgcc
     2101 gttgccctcc tgaaaaatga cgaccgaagg cttcaacagg tgcctctaat tgttctggta
     2161 aggcagcgac aaactctttt gcttgagcca attcaagagc ccgccacagt tcctcatcac
     2221 taataggcat tgattgtccc agtaggaggt tacttctaat cgttccttta aataattgag
     2281 ctttttgagg aacaagagca atcacatttc gccactcggt tagtgttttt ggcacttctc
     2341 cttgccaatt aagactaatc tcaccactat atggtgaata agtttggcag atcaagtcaa
     2401 tcaaagtcgt tttgcctgct ccagtaccac caatgatgcc aatccattct ccctggtcag
     2461 cacttaactg aatatcatac agacttggct ctgctgctcc tggataagta aaggtcaagt
     2521 gccggatggt caacagatga gttgaaatag gaccatcttt aagtgagggc aaaggcgctt
     2581 catcagctaa aacaaacacc tgattaatcc gttttgcggc tgttacactt tgattgatgg
     2641 ttcccatcaa catggtcatt ttcaaaagtt ctgccaaaat ctgcaagagg taattaatta
     2701 aagcaaccaa catgccctga ctaagcgatc tatgagctac ttgccaactt ccttgccaaa
     2761 tcaagattaa cagggtaaga ttgaccacta aaaaagtcat aggattcaag acattggcta
     2821 gggtagcagc ttgatattgg tggcgactta agagcatatt ttgctgctta aatgcttgga
     2881 gttctttttg ggtttgatta aaagccttaa ttactcggat tccttgtaac tgttggctag
     2941 tcaaacgaac caagtgatct gtttctcggc ggattaagag ataaagggga cctaataaat
     3001 gtgacataac aaaaacaagt gttagtaaga caatcaccat catcacaaac catagggtta
     3061 gacttggact aatccaataa gccattaccg tagcaccaca tacgataata ggggccctta
     3121 gaaaaagacg caaaaattga ttgataccag tttgaatttg aaaactatca ctggtcaatc
     3181 gtgataacag actagcataa ccaagatggt cttggtcctc cttgctcaaa aacatgattt
     3241 ttttaaacaa atcatctgtc atttgtcttg taaaaccaac agctgctttc gaagaaaaat
     3301 actgggctgt aacggaaagc aataagccaa tggttgctaa accaaataat gttaaaacaa
     3361 ccctcaaaat tccctggctg ttgtgttgac taatcgatat atcaatcatg ttagcaatta
     3421 acaaagggac taataattca aataaagctt ctaataattt aaaaagagga cctaagatac
     3481 tttcttttag gtaccctttc aaatatggac gtaatcgttt catgataact ttctaaaaaa
     3541 ggctcatcag ttcaattaaa taaagattga aatgaacttc tcgtgagtaa tagatattgc
     3601 cgccattctt ataaagctta cctccagaaa tcaaggcgag ggggtggtaa taaaaatagg
     3661 tttgaccact agtgttgcct aggcttggcg ccacaacggt tttggaatag gtagtcgcta
     3721 aatcaacggt attttgacct ccatgtttag caatataatc aatataagct gttccaaaat
     3781 tataggcctg aacagccgtc caagaatcta ctcctgcttc ctcagctaga gcaagattat
     3841 gagacagtaa attcaccccg tgctcaatac tggcctgact atctgtaatc gagttttttt
     3901 gtcctgaact gctttcactg gattgcatga catctgcttc tcctcctttt gtctcggtgt
     3961 atatcatagc taagactaag tctacatttg cctttgtatc attttctgct aatgttttct
     4021 caaccatagg cttatatgcc aaaacacgtt gaacattgtg atgaataaca aaggattgat
     4081 aaatgacaaa aagaagaaga aaactgcagg cacgctttaa aagtctaaac atctatttgg
     4141 ttttaatatc ctctatattt ttaatcaaga ttgaataagt cccatcatca tttaacaaaa
     4201 attcaaccgc ttcagcatcc tgataaatgg catttggtac tgttaattct atcccgtttg
     4261 ataacgatag cttttgattt tctagttttt ttatctgtct agaatgatca atatcgctaa
     4321 tttttatagg ttctggaatg acttctttga cttgatccac aaaggttaaa cgggctgtta
     4381 aattatcatc aaagagttgg tcagctaatt tttctggtga taaaacgtcg tctgcttcta
     4441 actgcttaaa aagagtggat ttcatctttg actgaaaagt aaaatcatct tgattaaaat
     4501 gctctgcaat tttttgagcg gtttgttcta tcattttgat agacttttta acagattgct
     4561 caggtgtaac tttcaagagg tgttctgaaa aataattagc aaaactgcca ttatgcttca
     4621 cacgcttttc aatcaagtag tactgaccag atttgagatt aatgactagt gcttcatctg
     4681 gtgtctgggt gggactcggc agattattct gcgtcaccgt aaatggatgt tcataattgt
     4741 cagaaaggtg agcaaactgt tctttcaaag caattcgcaa gaaagcaaaa aagggttcgc
     4801 catccttatc aaactggaca aagactaaat cattggtttt ttgatcctct gaaataacaa
     4861 aggcttcctt ccataactgg gcaatggtaa cagacgtttc caacaaatca tcaccaatgg
     4921 ttgtaaaaaa ggtattatta gcctcaaact gaccccgttt ggcttcatcc gagaacactt
     4981 tagccaattt tttccggaaa tattcatcaa ttcgtggtgt aatgctcact aaacgatctg
     5041 ataacagtaa ctcagtgtca ttgggagaaa attgatgaat gacaatacgt tttatataac
     5101 tatctaacat gattccttac tttttataga gtgggaaagc atcggttaac tgacgcactt
     5161 cctgacggac ttcttcaaga atagtttctt gatcatgatt aaccaaggct ttgatgataa
     5221 gacgggcaat ggtctgactc tctttaacac ccataccacg gctggttatg gcagcacaac
     5281 caatgcgaat acctgaggtt ttaaatggcg acaaggtttc aaaaggaatg gcatttttat
     5341 taagggtaat attaacctca tccagaagat tttgcgctag tttaccatta gcaataacct
     5401 tagtgacatc tactaaaaag acatgattat ctgtaccacc tgagatcaga cggaaacgat
     5461 catcttgtgc aaaaactgct gccatagccg cagtattatc aataatagct tgtgcatagt
     5521 ctttaaaggc tggatccaag gcttctttga aggcaaccgc tttggcagca atcacgtgtt
     5581 ccagaggtcc tccttgtaaa ccagggaaaa cggccgaatt aatcttttta gcaagagctt
     5641 cgtcattggt taatatcaag cctccacgag gaccacgtaa ggttttatgg gttgttgacg
     5701 tcacaatatg cgcataaggg acaggactcg ggtgcacacc tgctgctaca aggccagcga
     5761 tgtgagccat gtccaccatt aagtaagcac ctacgtgatc agcaatggca cgaaactttt
     5821 caaaatcaat gcttctagag taggccgaag cccctgcaac gataagcttt ggctggaccg
     5881 cctttgcttg ttctaaaatc gcctcataat ttaacatttc agtatcagca tctactgaat
     5941 aacccacaaa atggtaagtt tttccagaaa aatttactgg agagccatgc gtgagatgac
     6001 cacctgcagc caaatccatt cctaatacgg tatcaccagc ctcaattaag gccatataag
     6061 ctgctgcatt ggcttgactt ccggaatggg cttgaacatt ggcaaatgcg gcaccgaaaa
     6121 gtttcttagc acgttcaata gcaagcgttt cgacaatatc cacacattct gttccaccat
     6181 aatagcgatt acctggatag ccttctgcat acttattggt taaaacagaa ccttgagctg
     6241 ccatgacagc ttttgaaacc atgttttcag atgcaatcaa ttcgatatga tgttcttgtc
     6301 tttcttcttc agcatgaatg gcatcccaaa gctctttatc aaagtcttca acattacctt
     6361 tatcaaaaat cattgtcatc tccttgtaag tttttgatga atcgtaattg tgttttatca
     6421 tctgcatagt cgaggtttcg gtaaaaagca tgcgcttctt tacgatgact ggcagaattg
     6481 aggcgaataa aggcaatgcc ttcctgccta gcttgggatt ccagggccct tagtaaggca
     6541 ctcccaattc cccttctctg ataggctggt aaaacagcca agcccaagag attaagacca
     6601 tcagaagcat aaagggtctc ataacgttca gcgtgaacat agcctaataa ttgattggta
     6661 tccttgtcgg cataagctaa aaaatagtga tggcaatcct caatcaatcg ttcatattgc
     6721 ctttctaaca agtcaagcga aactaaatag ccaagggctt tggcgttaat ttcttgtagc
     6781 ataggacagt ccgttttact taatggtctc aacattccaa gcggctcctc tcttaaaagc
     6841 gaagttcagg cacgttggct aataattctt ctttggtgat agcaccttga cgtaaaatca
     6901 cagcacgctc acctgataag tctaagatgg ttgaatcctt acctgttaga aaaggatcat
     6961 ctgcataacc aaacacttga aagtcaaaat cttgcataat atgatcaaaa actcgaccac
     7021 tagctttgcc agataaattt gcagaaggac caattaaagg gcctgttttt tgaattaaag
     7081 ctgcggtaat tggatgattt ggtaagcgaa aaccaaccgt tgaaagaccc gaattaatcc
     7141 aataaggaac ctggtcatta gcttttaaaa taatcgttaa cggacctggt aaaaaggctt
     7201 ggtataattt cttgaggtat ctcggctgtt cttttgaaaa ggctaagata ctattaaaat
     7261 cagccacatt aaggttcatt gctttatctc ggggtctttg ttttaagtca taaacagcat
     7321 ttactgcttt ttcatctaaa gccttggcga aaagaccata aaccgtttct gttggcaaaa
     7381 caagggcatc acctgattca atgatactag ctagatactc cataatcaac aaccaccatc
     7441 ctttctttac caaatatatc cgttacgact cgtatatgtc tttgaggaaa ataggcttgt
     7501 aacatatctt ttataccttc agcttgctta taaccaattt caaagtaaag cttcccctcc
     7561 ttagctaagt aattgtcagc ttgttcaata atcttacggt aaatagcata gccattttct
     7621 ttagcaaaaa gtgccaaatg aggctctgat tgcaaaacat ttagactaac ttcttcctta
     7681 tcctcgtagg atatgtaggg aggatttgac acaataatat caaatgttcc tgaaatgaga
     7741 ctaaaaacat ctgattcaat aaacgttata tcaagctggt aagcatcagc attggctttt
     7801 gccaagtcaa gagcagctct agaaatatca gatgccgtta cttgccagtt agacctttct
     7861 tttttgagag aaatagcaat cgctccacta cctgtaccaa tatctaaaac attcaacggt
     7921 gcatcaagat tttcagctaa aatcatgtca actaattctt ctgtttctgg ccttggaatt
     7981 aatacacgct tgtcgactgc aagcttcaaa tcacgaaaat aagcattacc tgtaatatat
     8041 tgaggagata aatgctgact tagagaacag aaaatatgtt ccaaaagaac agcatcttca
     8101 ggagtcacag cttgattttg gtgaatcaac atatctagac ttgaccattc tttgatttct
     8161 ctaaaaacat aggcgagatt ttcgcgatcc tcatctattt gttctagttt atcttcataa
     8221 gttctgatta atgtagcata gttcattagt tattgagaga ttctaatttc tttgtctgat
     8281 catacataac gagtgcatca ataacttcat ccatttttcc agataaaata gtatctaatt
     8341 tttgaagtgt caaaccaata cgatgatctg ttacacgatt ttgtgggaaa ttataagtac
     8401 gaatacgttc tgaacgatca ccagtaccaa ctgttgattt acgttcagca tcttgctcat
     8461 cttgagcaat ttgcgcaaaa tggtcagcaa cacgagcacg aatgattttc atcgctttat
     8521 cacggttttt ttgctgagta cgctcttctt gcatttcaac tttgattcct gtaggaatgt
     8581 gtaccatacg aactgcagta gcaactttat tgacgttctg accaccagca ccagacgcgt
     8641 ggtaaatatc aatacgtaag tctttaggat caatgtcgta ttctacttct tcaacttctg
     8701 gcattaccaa aacagttgca gttgaagtat gaacacgacc ctgactctct gtcactggga
     8761 cacgttgaac acgatgagct cctgattcat atttaagctt agaataaact gactgaccag
     8821 aaaccatggc aaccacttct ttaataccac caacaccatt aacggatgat tccataactt
     8881 caaaacgcca tccttgagtt tcagcatatt tttgatacat tgttaaaaga tcaccagcaa
     8941 aaagcgctgc ctcatcgcca ccagcagcac cacgaatttc taaaataata tttttatcat
     9001 cgttaggatc tttaggtaat agaagaattt ttaatttttc ttcatactct tctttagctg
     9061 cttttgattc tttaagttct tctttagcca tttcttctaa ttcaggatca ccactggcat
     9121 ctttgatcat ttcttcagca tctgaaatgg tttgaatgac ttgcttatat tctctataag
     9181 ctgtgacagt ttctcgagta ttagcttctt cacgtgataa ttccataaat cgctttgtat
     9241 ctgaaacaac atctggatca ctaagtaact cacctaactc ttcataacga tcttcaactg
     9301 cctgcaattg atcataaata ttcatttttc tttgtatctc ctttaaaact gattaccttt
     9361 taaatctctg gattaaaata atgttttcga caaactggga tataggtttc attaccacca
     9421 atttgtatct gatcgccttc atagacaggt tttccgtttt ctgtccttaa aaccatggtt
     9481 gctttcttac tacagtattg gcaaattgtt ttgatttcat caatcttatc ggccaataat
     9541 agcaaatgtt ttgaaccttc aaaaagattg ttttggaaat catttttcaa accaaaagcc
     9601 atgacaggaa catttagctc atccaccaca cgcgccaaat cataaacatt ttgcttgctt
     9661 aaaaattggc tttcgtcaat caggacacaa taaggttttt cctccaattg ggcaataaat
     9721 gtgaaaatat ccatgtcatt tgaaatggga atagcttctc gtcgcatacc aatacggctt
     9781 gagacgatgc caaaaccatc acgtgtatca agagcacttg tcataatgac aacaggtttg
     9841 ccttgttctt catagttatg agcaactttt aaaatttcaa tggttttacc agaattcatt
     9901 gtcccatatt tataatagag ttgagccaaa gcggaaaatc ctactttcta tagtattcta
     9961 cctattttac cacaaaggct gaagttttcc cagccattga aaacagtgta tactttttca
    10021 cagcattatg ttacaatgat gcccaataac ataaaggagt aacattatgc catttgtaac
    10081 aattgacttg tttgagggac gctcacaaga acaaaaaaat caattagctc gcgaggttac
    10141 cgaagtagtc tcacgcattg caaaagcacc taaagaaaat atccatgttt ttatcaacga
    10201 tatgcctgaa ggaacttact atcctcaagg tgaaatgaaa caaaaatcat aatgaccttc
    10261 ccccactcct tagtgggggt attctgctaa aaagaggact aattatgttt gttacgcaac
    10321 aattaaagct aatgggaaca gttattgata ttcagatcga atctgacaaa gcttgccaac
    10381 aactaagtag agtcattgac ttattatata cttacaaaaa tcgtttttca gctaatgatt
    10441 caaactctga attgatggct attaatcagg cggctggtgt taaacccgtc agcgttcact
    10501 ctgatttatt taacctaatc caaattggta aagcttatag cttgtcaaca ccaagtaatc
    10561 ttaatatcgc cattgggcca cttgttcaag cctggcgaat tggatttgaa gatgctagag
    10621 tgcctagtca taaccttatc tcacaacagt tagctctaac agatcctaga caggttctta
    10681 tagatgacaa aaaacagact gtctttttac aacaagtagg aatggcttta gacttaggag
    10741 ccttagcaaa aggttatatt actgataaga ttatggccta cctgattgaa gatggtattg
    10801 actctgccct tattaattta ggtggaaacg tcttagttca tggacctaat ccgaaaagcc
    10861 ctgacaagac ttttcgaatt ggcattcaaa aaccagatgc taaacgtggg caacatcttg
    10921 gtgtcatcaa ggttaacaat cactctgttg tgacttctgg aatttatgaa aggcagttta
    10981 cagccaaagg gaaacagtat catcatatct tagatcgtca gacaggctac cctatagaaa
    11041 cagatatgct tagcttaacg gtcatggctc cttcttcttt ttattgtgac atatgggcta
    11101 cccgtttatt tggtcttgat agctcaatga ttatcacact tttaaacacc tttgataata
    11161 ttgaaggtct tctagtaaca agaaagcacc atgtccttat gtcaaatggt ttgagacact
    11221 attttcaacc atactatcat taaaaggggt taacggtttg ctcaaaactg atcacagaaa
    11281 aaacggctca attagccgtt ttttaattat tcatcttgtt tgtctttacc atttaaaact
    11341 aaatttaaga caacagatgt caaagtggca atcacaatac cattcgttaa gaacatttga
    11401 gctgtctctg gtaagctagt aaaaagattg gtgccattaa accctaagcc tgctgaaatg
    11461 gaaacagcag caataataaa attatactcg ttcttttgga aatcaacacg attaagcatt
    11521 tgcattcctt gaagagcaac cataccaaag agaacaagca tggctccacc aagaactggg
    11581 cttggaatca tttgtgccat tgctccaaat ttagggagta gaccaatgac cacaaggata
    11641 cctgctgcgt agtaaatcgg acgacgggtt ttaatacctg agatttggac aagtccaacg
    11701 ttttgagaaa aaccagtgta agggaaggta ttaaataacc caccgagaaa gactgcgatc
    11761 ccttctgagc gataaccatt gcgcaaacgt ttttcatcta attgatcatt agtcaaatct
    11821 gaaagcgcta agtagacacc tgttgattca accatagaaa ccgttgcgat gatacacatc
    11881 atcacaatag aagtgatttc aaaagttggc atgccaaaat aaaatggggt tggcacatga
    11941 atccaagaag cctctacaac aggagttgta tctactaatc ccatcatagc tgaaaccaaa
    12001 gttcccgcta caagcccaat caaaatggaa atggatttca caaaaccttt ggtaaatttt
    12061 tggactaaaa gaataatgac aatcgtcaac aatgataaca tcatgctttg tgccgtaggc
    12121 tctttgacgt tatctcccat attccccata gcaacaccta cgagacttaa gccaataacg
    12181 gtaatgaccg atcctgtaac aattggtggg aagaaacgag caatcttaga aaaaatacct
    12241 gccactaaaa tgacataaat tcctgacgca atgagggctc caaacatggc acttgaacct
    12301 tgttgcgctc caataatgga tagaggtgcc actgattgaa aagcacagcc taaaacaact
    12361 ggtaacccta ctcctgtatg cttggttaac tttaactgta aaaaagtagc cacaccacac
    12421 atgaaaatat ctgtcgaaat caggtaggtc aactctctag cggaataacc taaagcacct
    12481 gcgatcataa ttggtactaa aatagatccg gcatacatcg aaagcacatg ctgtaagcca
    12541 aggacagctg attgcgagtg agaatgttcc tgctttgttg aatgtgtcat tatgcgtcag
    12601 cctccaagaa gtttagatta ccattttcaa agtttttaat acgtgctaat gaggtcactt
    12661 caatgcccat atcttcaatc aatcgacgac catcttggaa agatttctca atcacaatac
    12721 caacgccgac gacttgtgcc cctgcttgac caataatctc gatcaagcct ttggctgcct
    12781 gaccattagc taaaaagtca tcaataatca aaaccttgtc ttctttagat aggaatttac
    12841 cagcgataga caccgtgctc gtcacttgtt tagtgaaaga ataaacttct gctgtcaaaa
    12901 tgccttctgt catggtaatg tttttatgtt ttttcgcaaa aatcatagga acatccattg
    12961 cttctgcagc gtatacggct ggtgcaatac ctgaagcttc gattgtaacc acttttgtaa
    13021 tgccagcctc agcatatttt tgagcaaaca ctttaccaat tgctttcatc aaccggtaat
    13081 caacttgatg agttaaaaaa ttatctacct ttagaatatt ctctcctaga atattgccat
    13141 ctgttaagat gcgttcttca agtaactgca taaatctcct tttgattcaa atagaaaaag
    13201 cctgccgcaa agccagaaac tataagagtt tctcactttg caaggttaaa aaccaagcaa
    13261 gcctaaaagc ttacttattg ttagacatta aggtgtcttg tagagacgct gcgccaattc
    13321 acagcataaa taagtatgtc attattgaat tcattatgat aatttactat tttaccatgt
    13381 ttcttatttt tttcaatacc tacagagata tgataaaagc atctccagca ttaagctaaa
    13441 aaattcttta ctggtcctag ctaactccat atcatttaaa tggaatcacc attccaaata
    13501 gaatttttga cgataacata atcaacatga cgcaaactgt ctaaatcttt accacccgcg
    13561 taagaaatgg acgattgcaa atcctgttgc atttcggtca gggtatctga taagtgacct
    13621 tttgtaggca ataagatttt tttgccttct acatttttat gctcgccttt ttgatatgct
    13681 gacgccgagc cataatattc tttaaacgtt tcaccattca cttctactgt ttttccagga
    13741 ctttcaatat gccctgcaaa aagcgaacca atcatgacca tactagctcc aaagcggatg
    13801 gatttagcga tatcaccatg tgtgcgaatc ccaccatcag caatgatagg cttacgagct
    13861 gcctttgcgc accagcgcag tgctgataac tgccaaccac ctgtaccgaa acctgtttta
    13921 actttagtga tacatacttt gcctggaccg atgcctactt tagtagcatc agcacctgca
    13981 ttttcgagtt ctcgtacagc ttctggcgta ccaacatttc cagcaatgac aaatgtttct
    14041 ggtaattcag ttttgatatg cttaatcata tcaataacac tgttagcatg accatgagca
    14101 atatcaatgg tgataaactc aggagtatcc tctttcaaag aagtcacaaa ttcatattcg
    14161 taagctttaa ccccaacaga gatggatgca atcaagcctt gttcatgcat gcgtttgata
    14221 aaaggtttgc ggctatcttc atcaaaacga tgcataatat aaaaataacc ctcttttgcc
    14281 aactgctcag caattgtttc atcaataatg gtttgcatat ttgctgggat aactggtagc
    14341 ttgaactggt attttccgag tgtcacactt gtatctgctt gtgaacgact agtaataatg
    14401 cacttgttag gaatcagttg aatatcttca taatcaaata cagggatgtc attaaacata
    14461 agtttctcct cttttagcac agatgaaaga aaataagtca gttctcacct tcttctctaa
    14521 gtcctctata caaaataatc ttcctatgac ctctgatgat tagaggtatc tttaggtaaa
    14581 aacttacctt tcattttata gattacttat ttgtttatat tttgtcaaat ttatatgcta
    14641 tttttaattt taaagttcgg tttttgtatt tatgaattgt tacttcttga acttaagaga
    14701 cgaaaaaatg ccagtcctac ggaccaacat ttctgtttat ttctggatta agcctgtttt
    14761 caacagataa tctcgagcga ccttattagc atctgctcct ttaacagata cctcgtagtt
    14821 catgtcctgc atctcttttt cagtaatttt tcccgctaat tgattaagaa tgccttttaa
    14881 ttctggatgt tttgttaata aggaagtttt catcaaagga gctccctgat aaggtggaaa
    14941 caactgttta tcatccttta aaactttcaa atggtatttc gtaatttcag catccgtaga
    15001 gtaagcatct gttacttgga tatctcctga ttgaattgct tgataacgaa gagctggctc
    15061 catagtcgcc acagatagct gtaatccata ttgagattgc attcccttat agccatctgc
    15121 tctgtcctta aattccagag taaaaccagc ctttaaagta tcagcatgcg cttttaaatc
    15181 agaaatggtt tcaatctgat attccttagc caatttttct ggcatagcaa cagcatacgt
    15241 attttggtaa gcaaatggct taagaagggt cagtttatct tgcttagcaa tgcctttttt
    15301 agcatcctca tagacctgct tagggtcatt agacaaaggt ggtttgtcgc gtaaaagact
    15361 tgatgttatg gttcctgtaa attcaggata gatatcaata tctccagatt taagggcttc
    15421 atataagaag ctagttttcc caaaattaga tttcaactca acacttatat ctgattggtc
    15481 ttcaattaat tccttataga gattaatcag aatatcgggc tctgctccca atttaccggc
    15541 aataaccact gtatcttttc cttttgaaaa ctgactaagc gccataggag tatacgatgc
    15601 tagcaatgca agtaaggtga ttccaaaact aatcataatc cgtcgcaagg aagctttctc
    15661 aaggtactgt aacaaactat tgaaaataat agctagcaga gctgaagaaa tagcccctat
    15721 cagaatcagg ttagcattat tacgatcgat ccccaataag atgaaagagc caagtccacc
    15781 ggctcctatc aaggaagcta aagtagctgt gccgataatc atgactgctg acgttcgcac
    15841 acctgacata atgacaggca tggcaattgg aatctcaaac gtttttaacc gctcccattt
    15901 ggtcatccca aaagctattc ctgcttccac aagacttggg tcaataccat ttaatcctgt
    15961 gatggtgttt tgtaaaatcg gaaaaatcgc atagataact aaagctgtca ctgcaggcaa
    16021 cgttccaatt cccattaaag ggatgaaaag accaagcaaa gccagtgagg gaatggtttg
    16081 aaaaacacct gttacctgta acataatgtc tgaccaacgt ttgctgcgac tgagaagggc
    16141 tgctaaaggc acacctatta gcaaggcgat cataagagat aaaagggaaa tttgcaggtg
    16201 ttcccccaga gccgccagcc attcattaaa acggttttgg aaagttacaa acaaactagg
    16261 catgctcatc acctccaaat aaattgacca caaaagcatt agcagggtgg gtcttaatca
    16321 tttctgggcg atccagttgc acaatttctc cctcatttaa aatagctacg cggtctccta
    16381 atttaatggc ctcatcaata tcatgtgtca caaatacaat cgtcatgtca aattccttgt
    16441 gaagactcaa cattaactct tgcaattgtt tacgtgaaat aggatctaag gctgaaaatg
    16501 gttcatccat caatagaatt ttaggatgag aaatgatagc acgtacaata ccaatacgtt
    16561 gctgttcacc tcccgataaa tcacttgggt aacgatcaag ataatcttta gcaggtaggc
    16621 ccaccttatc caataattct tcagtttttt gtctaatttc ttcagctgac cattgcttca
    16681 tatcagggat aatcgcaata ttttcagcta ctgtgagatt ggggaaaaga gcaatttgct
    16741 gcaacacgta tccaatagat agcctcatct cacgtaaatc taactctttc tgtgggacgt
    16801 tattcagtaa aatatctcca gagcttggtt caataaggca gttaatcatt tttaaaagtg
    16861 tggttttccc cgatccgctg gggccaacca gcacaaaaaa ttcacggtca ttaatttgaa
    16921 aggtctgctc ttgcaaaacc tttgtctgac caaaagtttt agaaacatta ttaaaacgta
    16981 tcattatctc tccaattcat tggttaaagt aacagcataa aagctgatga gttcattcaa
    17041 cctgtctctc tgtcattttt tctcaaaacg ttctgagtaa ctacaaggaa tcactgcatg
    17101 ccctaccatt tttagacaaa cgcatctact aattaaaggg actcttaggt atcctcagta
    17161 aggtaaaaac taccaattct caaaaacatt ttctctcatt attttcttgt cgtttgttgt
    17221 tgaccattat aaaaagtttc ccactataag tcaaataatg agactattac ccccttatag
    17281 aaaaacaggt cactgggtca gcctacctct ccaaaattac cttatcaaaa atcctagact
    17341 tgcctatgat agataagcag attagttgtc ttttctagaa aatacggcta tcatacaatt
    17401 atgaaaaatg ccatttccat tttagcgctc ttaacacctg aaaaccaact tgaagatacg
    17461 tttattagag agctttccca ccacttctca cacctttttg aggtgactat tctcactagc
    17521 aaagccaaca ttgagtcaaa tcaattgtcg actttccaag gaattttcag tttccatgag
    17581 catgacattg acttgcctac actctatttc aaaacctctc aacatggtca gggattttta
    17641 gtgacagaaa gtgtctttga tcaagcaaca gcagtcttat cgttaagcca atacctcact
    17701 agattttacc aaaaatttga tagacatttt ttagaatacc ttcctttgca agcaaggtta
    17761 tctgacgcaa aggggaacat tctagtcgat aatcatgctt ttaatggaag ttttttgcca
    17821 actactgata acgaaataga agattggatt cttgctgaat tgcgtttatc agataaccct
    17881 tgtaaaacct tcctacttcc ttcgggttca ttggatcata tttacatgca gcattatcaa
    17941 gctcttaaaa atcctcaagg tcagctggtg ggagttttgg ataccgtcca agacattaaa
    18001 ccgttattaa gccagtattt agaagaaact ggacaagcca ttgttggctg gtccgatgtg
    18061 acatcaggtc cttccatttt agatcactaa acttgatttt aatagggagt ccagcctaaa
    18121 tcatcaacgc acaagaagca atcaagaaca cttggaaaca tcaaaacctt cttcttgact
    18181 gctttttttg atgactaaat cggtaagtct cacttataaa gaataatcca ttccagtaaa
    18241 ccatttttct aaagattatt attcttatgg actcatccct tgctagtcac gagtaactgg
    18301 ttgcggtcca taactttgcg acactggcat cagctcaata cgattaacat taacatgagg
    18361 gggctgatga atcacccatg atacagtcga cgcaatatct tgaggttgaa tggcatgtgc
    18421 tccttgataa atggccttca cccgtgcctc atcaccatta aagcgaacct ttgaaaattc
    18481 tgtcccttca catagaccgg gttcaatatt ggtcacacgt atatggctac ctgctaaatc
    18541 agctcgtaaa ttgagtgaga actgcttaac aaaagccttg ctagccccat aaatattagc
    18601 tcccggatag gaaattgtcc ctgcggttga ccctaaatta ataataatac catcatcttt
    18661 ggagaccata tgtggtaata attgacgtgt gagataaatc aacccaacaa tattagtgtt
    18721 aatcatcgtc atccaattct caaaatcggc ttcataagcc ttatcaagtc ccaatgccaa
    18781 gcctgcatta ttcactaaaa tagtaatatc ttgccactga tttggtagac ttgtcaaagc
    18841 atcatcaatc actttggtat ctgtgacatc catctgcaaa gggtaaaagt tgtccgaccc
    18901 aaagctcgtt tctaaggcct tgagtttctc aagacgacga gaagcaccaa taacctttat
    18961 cccatctgca attaagtgtt cgacaatggc tctgccaaaa ccggctgaag cccctgttac
    19021 taaggcaata tttcccatac atttcctcac cttcaaaaaa aacgagatgg tctggccaac
    19081 tcgtttttaa taatgtcaat tagtcttagg acaacttcta acacatcact tagcggttag
    19141 gatcctatat caaggggtct taagttaccc ttggctttca atagcttgtg ctgctgtaat
    19201 aatagctaat ttgtaaatat cttcagcgct tgagccacga gataaatcat taacaggttt
    19261 gttcaatcct tgtaagattg gtccaatagc atcaaacatt cctaaacgtt gtgcaatttt
    19321 gtaaccaata tttccagatt gtaagtcagg gaaaataaaa gtattagctt gaccagcaac
    19381 tgctgaatct ggtgctttga tagctgctgt ttcaggaaca aaggctgcat caaattgcaa
    19441 ctcaccatca agtgccaaat caggattcaa ccctttagca atttctgttg cttctcttac
    19501 cttatcaact tgaggcgctt taccagaacc ttttgttgag aagcttaaca tagcaatttt
    19561 aggatcaata tcaaagattt tagctgtttc agctgtgtta acagcgattt ctgccaattc
    19621 ttgtgctgtt gggtcaatat tgatagcaca atcagcaaac acgtaacgct cactcgtatt
    19681 ttcgcgattc atcaagaaaa cacctgaagt tcttgaaata cctggtttgg ttttaatgat
    19741 ttgaagggct ggacgaacag tatcagctgt tgaatgaata gcaccagata ccattccatc
    19801 tgctaatccc attttaacca acataacacc aaagtaattc acatcacgaa gcattttatc
    19861 agcatcttca agcgttgctt ttcctttacg gatgtcaaca aaagcttctt tcattttgtc
    19921 aaaatccgca tattcatttg gattaatgat agtatagtct tgatcagcga aacctaattt
    19981 tgtcaaaagg ttgcgtacct cttctgactg tccaaggata atcggttcta agagtccttc
    20041 aaatttcaaa cgagcagctg cacggacgac gcgctcatca ttaccttctg ggaaaacaat
    20101 cttcatgttt ttaccaagaa ttttttctct taaaccaccg aataaactac gaatactcat
    20161 tctttaataa cctctatctt ttttctgtaa acctatgata acgctatcaa agtcatctgt
    20221 caagtgtatt gcatagctac aatcactttc tgtaaatgga tctttgaaat tcaaataatg
    20281 acaatgtaaa gcttgccttg ttattcctaa gtccaaacga cctccgtaaa ggtcatcccc
    20341 taaaagtgga aaaccaatat gggcaaaatg aactctgatt tgatgggtac gtcctgtatg
    20401 caatttgata tccactaaat gcacattttc tgaatagcgc gccacaacct tatagctagt
    20461 cttggcatat ttgcccatag ggtcaactgc cctggtaata atactatctt ttgaacgccc
    20521 aattggagca ataatatctc cttcatctgg caacgtccca tttccagaaa ccagagcaaa
    20581 ataacgtttc tctattgagc gagtttggag ttgcttgtcc aaccttgcat gagcataacc
    20641 gtgcttagca aataacatca agccactcgt atctctgtct aaacgagtca cgatatgcac
    20701 ttgttggtct aggtaatgat tgtccacata atatgccttg ataaagttag caatggtatt
    20761 ggagtggatg gcacttggaa tactggcaaa gccaatgggt ttattaatca ccaaaaaatg
    20821 atcatcctcg tgaacaatat ccaaatcaaa agggatggct tcaagctttt caaagggctc
    20881 ttcgtttgga atgtcaatgg tgacaacatc tcccacttgc aataaataaa tggcattttg
    20941 ttcaatacca ttcactaata tattaccgcc cttatatttg attttagcca atagtccttt
    21001 agagacatca tgacttttta aaagggtttt tacttttatt cgtttatctg ctacaaattc
    21061 aaatctcatg agtcaatctc tccaataaag gcatccttga ccctttccca aaagctcgta
    21121 tgactcggag aggaaacaaa atgaattttc tcatcgtcga taaaatactc caccttcgtc
    21181 acatttttta actgataggt tttattatca atggaaatgg tataaattcc taatcgtttt
    21241 ggcactaact caatcttatc ttttttggga ataatgattg atgagcccaa ggttctaaag
    21301 acacggttat taagactgga aatttccgtc aattgcagcg cttcaatcgt cggatgcaag
    21361 acagcaccac ctaaagattt attgtaggct gtgctccctg tcggggtcga tactgaaatc
    21421 ccatcacctc ggaagctttc aaatttgaca tggttaataa taacatctgc taccatcgtt
    21481 ttttcaatac gcttaaccgt cgcttcattc aaagcacgcg ctttaaccac acgaccatca
    21541 tctaaagtaa tagcaacttt taaaatagga taagagattt gttctccctt gtcttttctt
    21601 aaattatcaa tcaatttatc aacttcaaaa tccctataat cggtataaaa gccaagatga
    21661 ccggtgtgga ttcctacaaa acgtacctta tctaattctt tttcatacat gtgaaaagca
    21721 gataagagca tcccatctcc gccaatagaa atcacaatat ccggattttt ctttgaaaga
    21781 tagaaatcag gatcatcttt aaatacggag aaaagtttgg aggcaacgcg tttactttgg
    21841 tacttaccat ttgcaataat agcaactcgc tttaccttac ctgtataatt catctgtgtc
    21901 atcactattt cctacaccat cacttaaatt tcgtgtcacg gggtcaaaca ataattgagc
    21961 ctcccgaata tcttctcgaa tcttacgcat ttcttcgtct aattctaagg caattttagc
    22021 agtcacttca agacgctttt taatttcttc tgggaagtct ccaccatatt tataatttaa
    22081 agaatgctca atggtcgccc aaaagttcat tgccaaagta cgaatctgaa tctccgctaa
    22141 gacctttttt tgaccctcaa tggtatcaac agggtactca accaccacat gataagacct
    22201 gtagccactg gacttcatat tgcgaatata atctcgttca taaacaattg tcatgtcttg
    22261 tcgttgacgc aatagagcta agacttcttc cacatcatca acaaactgaa ccataatacg
    22321 tagtccagca atatcttgaa tatcttgtgc tatattttct tcaataacac cacgtaaaat
    22381 cattttttct ttaatacttt caattgactt aacacgtccc gtaacaaatt caatcggaga
    22441 ataacgattt tgcttacgat attgcttgcg aataccccgt agttttattt ttaattcacc
    22501 aactgtttgg atataaggat ctaaaaattc ttcccaatct aaggtcatct attgtctcct
    22561 ttcttgtcaa agccaggata ataatataaa attgaaataa caatttatta tagcacaaat
    22621 taaaaggaat ttcccatgac taacctagaa atcgaatata aaactctact tacaaaaaat
    22681 gagtataacc gtcttttatt tcaaatgaaa catgtgacgc ctgttacaca aactaattac
    22741 tatattgata caaaagcatt tgatttaaaa gctaacaaaa tgtcattacg tattcgaaca
    22801 tttgtaaata gcgccgaatt aaccttaaaa gtgccagaaa aagtggggaa tcgtgagtac
    22861 aatgtccctc tttttcttga acaagcaaaa gatatgatta aacatgggaa tcttcctgaa
    22921 tcaactgctc ttgatattat tatttcaaaa ggtatcaaac catctgcact ggtaactttt
    22981 ggcaatttaa ctaccgttcg tcgcgagaca gtgataccga taggtaaatt ggccttagac
    23041 tataacctat atgccaatac caaagactat gaattagaat tagaagtaag tgacgctctt
    23101 caaggaaaga ttgattttga ttctttttta tctgaacatc acattacttt caaatatgct
    23161 aaaagcaaag tagctcggtg tattaatacc ttaaaaaagt tcaacgataa ataatggcta
    23221 aaagcgacag aaaaactgaa atttttctgt ttttctgtta aaatagggat gtcaattaaa
    23281 cacttttggg taatgtgact cattaataaa acagtttacc caattttatt gaagttaagg
    23341 agctaaatct atcatgactg aacgatatgc tgacaagcaa atcaaactgt tctcactcac
    23401 atcaaatctt ccaattgctg aaaaaattgc taaagctgct ggaatccctc ttggaaaaat
    23461 gtcttcacgt caattttccg atggagaaat tatgatcaat attgaagaaa cagttcgtgg
    23521 agatgatatc tatattattc aatccactag ttttcctgtc aatgataatc tttgggaatt
    23581 actcatcatg attgatgctt gtaaacgtgc aagtgctaat accgtcaata ttgtattgcc
    23641 atactttggt tactctcgtc aagaccgtgt cgctaagccc cgcgaaccga ttactgctaa
    23701 actagtagct aatatgctga ctaaagctgg tattgatcgt gtagtgacgc ttgacttaca
    23761 tgctgttcag gtacaaggtt tttttgatat tccagtggat aacctcttta cagtccctct
    23821 ttttgcagaa cgttacagta aattaggctt atcaggttct gatgttgttg tcgttagccc
    23881 taaaaattct ggaattaaac gtgctagaag cttggctgaa tatcttgatt ccccgattgc
    23941 tattatcgac tatgcacaag atgattctga acgtgaacaa ggttatatta ttggtgatgt
    24001 ttctggtaaa aaagccattt taattgatga tattttaaat actgggaaaa cctttgcaga
    24061 agctgctaaa attcttgaac gctcaggcgc tactgatact tacgcggtcg ctagccatgg
    24121 cttatttgct ggtggagctg ctgatgtatt agaaacagct ccaatcaaag aaattatcgt
    24181 tacagattct gttaaaacta aaaatcgtgt gccagaaaac gttacttatc ttagcgccag
    24241 tgatttaatc gcagaagcta ttattcgtat ccatgaaaga agacccttaa gtccactttt
    24301 ttcttatcaa cctaaaggca aaaataacgc atgacttact ttgataacgc cgccaccaca
    24361 ccacttagtc ctaatgtgat tagggcgatg acagcagcta tgcaagataa ctttggtaac
    24421 ccctctagta ttcatttcta cgggcgccga gccaataaaa tccttcgtga atgccgacaa
    24481 gctatcgcta gaaacttggg ggcaagtgaa cagcaaatta tagtaacgtc cggtggaact
    24541 gaaagcaata atatggccat aaaaggctat gctttagctc atcaagcaaa gggcaaacat
    24601 ctcatcacaa ctactattga gcaccactct gttcttcata ccatggccta tctcgaagag
    24661 cgatttggtt ttgaggtcac ttatttgcct tgtcaaaatg gacaaataaa tttatctgac
    24721 ttaaaacaag ccttgagaga tgacaccatt ttggtaagca taatgtatgc caataacgaa
    24781 acaggtgacc tgttacctat taagggcatt gggaatctgc tcaaagacca tcaagctgct
    24841 tttcatgttg atgccgttca agctgttggc aaactcaaga ttattcctag cgaacttggg
    24901 attgactttt tatctgcttc tgctcacaaa tttcatggtc ctaaaggatg cggcttttta
    24961 tacagtaacg gacaaccaat cgaccctctt cttcacgggg gtgatcaaga aggaaagcga
    25021 cgcgcaagta ctgaaaatat gttaggtatt attggtatgg cacaagcttt gactgatgct
    25081 atgacttgtc ttgaccaatc aactgatcac attatcagtt tacgtcatca cttgattagc
    25141 ctcctagaag gactacccta ctatattaat caaggtactc attatcttcc tcatgttctc
    25201 aatattggat ttttaggcta ccaaaatact attttactaa cacaacttga cttagcaggc
    25261 attgcagtat caactggttc tgcttgtact gctggagctg ttaatccaag tcatgtttta
    25321 gctgcgtact atggggatga ctcttctcgt ttaaaagaat caattcgtat cagtttttca
    25381 gatcaaaata gcattgaaga tgttaatcaa ttagctcaaa cattaaaaaa tattttagga
    25441 ggaacagatg gcttttgaaa aagaaattaa gcttaaagac tgtaaatacc tttatacaat
    25501 cagtccaaac attaaaaaat ataccctaag agatactaca tttagtcaaa ctaaagtagg
    25561 gcactaccaa ttaatacgtt tactcgaaac aatacctaat tcaggggatg gtttcccttt
    25621 aaaaatcact attaataaag aactcacagg ttttaaatta gctatcaccg atcaatctgg
    25681 tcttagatta gttaatattt ttaaaacaaa agataatcaa atccttcaag ataaatttta
    25741 tttcttgatg gacagccttg tagaacgtga aatttttagc aaaacagtcg tctaatcaac
    25801 tttaaatttt gtttttttag tcatatcaca atgtcaataa atgatttcgc ttctattgct
    25861 attaattcat gttgttcatt ttgactgtca taacttactt tcctaatgaa tactattata
    25921 tagtgctaaa actaatttta gcttttttaa ctcatacaaa aacaatcaaa atgttgcatt
    25981 tttcacaatc tttttgttac aataaagaca gacatattta acgagaaatc tacaaggagt
    26041 aaaatagtgg ttattgacaa atctattcca aaagcgactg caaagcgctt atcactttat
    26101 taccgtattt ttaaacgatt ccatgctgac caagttgaaa aagccagttc taaacaaatt
    26161 gcagatgcaa tgggaattga ctctgctact gtacgccgtg acttttctta ttttggagaa
    26221 ttggggcgtc gtgggtttgg ctatgatgtt accaaattga tgaatttttt tgctgacttg
    26281 cttaatgatc actctacaac aaacgttatc ttagttggat gcggtaatat agggcgtgct
    26341 ctattacatt accgcttcca tgatcgtaat aagatgcaaa ttgccatggg ttttgatacc
    26401 gatgataacg ctcttgttgg tactaaaaca gctgataata ttccagtcca tggtatctca
    26461 tctgtcaaag aacggattgc gaacactgac attgaaacgg ctatcttaac ggttcccagc
    26521 attcatgctc aagaagttac tgatcaactt attgaagcag gaatcaaagg aatcttgagt
    26581 tttgctcctg ttcacctaca agttcctaaa ggagttattg ttcaatctgt cgatttgacc
    26641 agtgagctcc agacactact ctattttatg aatcaaaatc atctagatta aggaggtata
    26701 atgaccaaac cgattattgg aataactgca aatcaaagac taaatatggc tttggataat
    26761 ctcccatggt catatgcacc tactggattt gtacaggcag tcactcaatc tggggggctg
    26821 ccgcttcttt tacccattgg tgatgaagcc gccgctaaaa cttatgtatc tatggttgat
    26881 aaaatcattt taattggtgg gcaaaacgtt gacccaaaat attatcaaga agggaaggct
    26941 gcttttgatg atgatttttc accagagcga gacacatttg agctagctat catcaaggag
    27001 gctataacat taaaaaaacc aatcttaggg atttgtcgtg gtacacagtt aatgaatgtt
    27061 gccttaggag gaaatcttaa ccaacacatt gactcacatt ggcaagaagc gccttctgat
    27121 ttcttatcac acgaaatgat tattgagcca gactctatct tatatcctat ttatggtcat
    27181 aagacattaa ttaattcttt tcatcgccaa agtttaaaaa ctgttgctaa ggatttaaaa
    27241 gtaatcgcta gagatccacg tgatggtacc atcgaagcag tcatatcaac caacgatgcc
    27301 attccttttt taggtgttca gtggcatcct gaacttctcc aaggtgttcg agatgaagat
    27361 ctacaattgt tccgcttatt cgtcaatgac ttctgactta aaaaagggtt gatttttctc
    27421 gaaaactata ataatcttta tagctaacga taatgtgatc tagacagata atgcctaaat
    27481 cttcacatga tcgttttatt ttttcagtaa agcaataatc gttagaacta ggttcaatat
    27541 ttcctgaagg atgattatga ataacaatga gactagtcgc catattttta caggcatagt
    27601 ataaaatttc tcttggttct gcaagtgaac gtcggacagt cccaataaaa atagtttttt
    27661 cttcaataat acgattttga ttatctaaat acaatacgac taaatgctct tgttttttat
    27721 ctcctaaagc tgccatcatt ttttcagcga cctgaacact ggttaatacg ctatcagtta
    27781 atgtcttatc tgtcgctaaa attcgggaaa ccaactcaat catagcttta atttcaatcg
    27841 ctttaacttt tcctatacct gccaaatgtt gtaattcttg caaagacatc tttttaaaat
    27901 ctgccagact gtctaaatgc gataaaagat aggatgacag ctctaagaca tgcttttctt
    27961 tattacctgt tcgtaataaa attgctaata attcttgatt acttagagac tctgctccta
    28021 gtcgcatcaa acgttctctt ggcatggctt tattatcatc acattttata gaatacattt
    28081 atcatattac ctccatcttt ttattcgaca aaaaaacaag ttc
//
LOCUS       DAUCLR010000016        27779 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.016, whole genome shotgun sequence.
ACCESSION   DAUCLR010000016 DAUCLR010000000
VERSION     DAUCLR010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27779
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.016"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            210..2617
                     /gene="cas3"
                     /locus_tag="VGT87_000543"
                     /pseudo
     CDS             210..2617
                     /gene="cas3"
                     /locus_tag="VGT87_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
     gene            2750..3478
                     /gene="cas5c"
                     /locus_tag="VGT87_000544"
     CDS             2750..3478
                     /gene="cas5c"
                     /locus_tag="VGT87_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5"
                     /protein_id="HEQ3813426.1"
                     /translation="MYRSRDFYVRVSGQRALFTNPATKGGSERSSYSVPTRQALNGIV
                     DATYYKPTFTNIVTEVKVINQIQTELQGVRALLHDYSADLSYVSYLSDVVYLIKFHFV
                     WNEDRKDLNSDRLPAKHEAIMERSIRKGGRRDVFLGTRECLGLVDDISQEEYETTVSY
                     YNGVNIDLGIMFHSFAYPKDKKTPLKSYFTKTVMKNGVITFKAQSECDIVNTLSSYAF
                     KAPEEIKSVNDECMEYDAMEKGEN"
     gene            3478..5373
                     /gene="cas8c"
                     /locus_tag="VGT87_000545"
     CDS             3478..5373
                     /gene="cas8c"
                     /locus_tag="VGT87_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HEQ3813427.1"
                     /translation="MDFFTSLLKTYEKAELADLVDHQKRNNEPVLLPIYHTSLKSNGK
                     NIISVKLDKDGQFHKAEFMVDKQMIIFPVTADSVARSGSHPAPHPLVDKFAYYSAEMG
                     QSQYDSFHKQLNNWIDYCEEGDVKRFLTIVQQFILKPEFLTLILDSLIGPDYQHNQLK
                     VTFCDATGKEKLIDLSACFLEFSIDQFQGFKNESVSTFKALHQSYISFVEANRENLGI
                     CNISGREEQLTDKHRGLMGNAKIISVSNKREAYKGRFREREDVFSVGYETSEKIHLML
                     KYLLENKNTITWLGSSQYLINWFSDDLTNDSRLDIVSPIFDDGLEEDDDDDTPPVITL
                     ATEDNKRIGKSFIKGQKLFANDATYYVAILNKTSNGRIALKYFRQLQASQLLTNLNKW
                     QETYSWESRSKFGKSRLRTPTVHDILNVSYGVDRDRFLELDNDNFKSDQIQKLVASLI
                     DGKPMPQSIVKKLGNNVKERHRYRKHWYQVEQVCLAILHKQNGEEFSPMLDHTNQNRS
                     YLFGRLLAIFELIETLRYGLDGNNNDRITNAERYWTAYTGQPTKLMMLLENKIKPYEE
                     PLKLNRRGSWMKLEKEKEEILELLNPLLETETMEKPLDYRFIFGYYAEKNYYYTKQNT
                     EVTESEE"
     gene            5378..6226
                     /gene="cas7c"
                     /locus_tag="VGT87_000546"
     CDS             5378..6226
                     /gene="cas7c"
                     /locus_tag="VGT87_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HEQ3813428.1"
                     /translation="MLEHKIDFMVTLEVKEANANGDPLNGNMPRTDAKGYGVMSDVSI
                     KRKIRNRLQDMGKSIFVQANERIEDDFRSLEKRFSQHFTAKTPDKEIEEKANALWFDV
                     RAFGQVFTYLKKSIGVRGPVSISMAKSLEPIVISSLQITRSTNGMEAKNNSGRSSDTM
                     GTKHFVDYGVYVLKGSINAYFAEKTGFSQEDAEAIKEVLVSLFENDASSARPEGSMRV
                     CEVFWFTHSSKLGNVSSARVFDLLEYNQSIEEKSTYDAYQIHLNQEKLAKYEAKGLTV
                     EILEGL"
     gene            6228..6902
                     /gene="cas4"
                     /locus_tag="VGT87_000547"
     CDS             6228..6902
                     /gene="cas4"
                     /locus_tag="VGT87_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HEQ3813429.1"
                     /translation="MVYAEDDYLMLSGIQHFQFCKRQWALIHIEQQWLDNEATAHGQV
                     LHTKADNPYIKEKRKELLVSRAMPISSAELGLSGIMDVVEFYKDDQGVSLRGKRGKWL
                     PKVVEYKRGKPKKDTRDIVQLVAQTMCLEETLDCDINEGCLYYHSVNQRVIVPMTSAL
                     RQEVKELAAEMHEVYQSQMLPKAAYFKNCQLCSLVDICKPRLSKKTRSVSRYINEAMT
                     SEEMDL"
     gene            6899..7924
                     /gene="cas1c"
                     /locus_tag="VGT87_000548"
     CDS             6899..7924
                     /gene="cas1c"
                     /locus_tag="VGT87_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1"
                     /protein_id="HEQ3813430.1"
                     /translation="MKKLLNTLYLTQEDFYVTKEGDNIVIKQEGKVLKRFPFRIIDGI
                     VCFSYLGVSPALVKLCTENQIKLSFHTPQGRFCGRYIGSTNGNVLLRREHYRLSDREE
                     SLEYAKRFILAKISNSRKYLLRFKRDHRQQIDTKLFEAVNDELIWALEMVQAADNKDS
                     LRGIEGQAANQYFRIFNDLVLTDKKTFYFQGRSKRPPLDCVNALLSFGYSLLTFECQS
                     ALEAVGLDSYVGFFHTDRPGRASLALDLVEEFRSYIVDRFVFSLINKGQLQKKHFEVK
                     ENGSILLTENGRAIFIDLWQKRKHTEVEHPFTKEKVKLMLLPYVQAQLLAKAIRGDLE
                     SYPPFMV"
     gene            7935..8228
                     /gene="cas2"
                     /locus_tag="VGT87_000549"
     CDS             7935..8228
                     /gene="cas2"
                     /locus_tag="VGT87_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002280569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEQ3813431.1"
                     /translation="MMVLVTYDVNTETPAGRKRLRHVAKLCVDYGQRVQNSVFECSVT
                     PAEFVDIKHRLTQIIDEKTDSIRFYLLGKNWQRRVETLGRSDSYDPDKGVLLL"
     repeat_region   8377..8804
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=8377..8408
                     /rpt_unit_seq="gtctcacccttcatgggtgagtggattgaaat"
     gene            8964..9674
                     /gene="ccdA2"
                     /locus_tag="VGT87_000550"
     CDS             8964..9674
                     /gene="ccdA2"
                     /locus_tag="VGT87_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEQ3813432.1"
                     /translation="MTLSLVLMVSVFGAGLLSFFSPCIFPVLPVYLGILLDADDSKTI
                     TIFGKKLYWYGIVKTLAFIFGLSTIFVILGYGAGFLGNILYAVWFRYLLGALVIILGI
                     HQMGLITIKSLQFQKSLTFHNNKNRNGLFNAFILGLTFSFGWTPCVGPVLSSVLALVA
                     SGGNGAWQGGVLMIIYTLGLGIPFLLISFASGIVLKQFNKLKPHMLLLKKVGGVLIIV
                     MGILLMTGTLNNLAQLFG"
     gene            9687..10310
                     /locus_tag="VGT87_000551"
     CDS             9687..10310
                     /locus_tag="VGT87_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEQ3813433.1"
                     /translation="MKKGLLVTTGLACLGLLTACSTQDNMAKKEITQDKMSMAAKKKD
                     KMSTSKDKSMMADKSSDKKMTNDGPMAPDFELKGIDGKTYRLSEFKGKKVYLKFWASW
                     CSICLSTLADTEDLAKMSDKDYVVLTVVSPGHQGEKSEADFKKWFQGTDYKDLPVLLD
                     PDGKLLEAYGVRSYPTEVFIGSDGVLAKKYIGYAKKSDIKKTLKGIH"
     gene            10353..11459
                     /gene="msrB"
                     /locus_tag="VGT87_000552"
     CDS             10353..11459
                     /gene="msrB"
                     /locus_tag="VGT87_000552"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEQ3813434.1"
                     /translation="MEHKWKLWLFIVGMVLLIGGASVLIQKGQAKAFSQGKPQLPAKK
                     VKSTKADPNAPVDQKMIYLAGGCFWGVEEYFSQVDGVLDAVSGYANGRGDTTNYQLIH
                     QTGHAETVEVAYDANRISLKELLLHFFRIIDPTSLNKQGNDRGSQYRTGIYYTDKADL
                     AIIDEVFKEKAKDYKKKIVVEKAPLKHFIKAEEYHQDYLKKNPNGYCHIDINQATYPV
                     IDESKYPKPSATEIKAKLSADEYRVTQKNETEKAFSNRYWDSFDAGIYVDVVTGEPLF
                     SSKDKFESGCGWPSFSRPISPDVVRYKEDKSFNMTRTEVRSRSGNSHLGHVFTDGPKD
                     QGGLRYCINSLSITFIPKADMEAKGYGYLLSSVE"
     gene            11547..12287
                     /locus_tag="VGT87_000553"
     CDS             11547..12287
                     /locus_tag="VGT87_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEQ3813435.1"
                     /translation="MYSLLIVEDEYLIRQGIRSLVDFSQFKIDRVNEAENGQLAWDLF
                     QKEPYDIILTDINMPKLNGIQLAELIKQESPQTHLVFLTGYDDFNYALSALKLGADDY
                     LLKPFSKADVEDMLGKLQQKLELSKKTETIQELVEQPQKEVSAIAMAIHERLADSDLT
                     LKSLAQQLGFSPNYLSVLIKKELGMPFQDYLVQERLKKAKLLLLTSNLKIYEIAEQVG
                     FEDMNYFSQRFKQLVGVTPSQYKKGGQA"
     gene            12284..14017
                     /locus_tag="VGT87_000554"
     CDS             12284..14017
                     /locus_tag="VGT87_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ3813436.1"
                     /translation="MKRYPLLVQLISYVFVIVIALITTLGLLYYQTSSRNIRQLIERD
                     TRQSIRQSSQFIDAYIKPLKETTSVLAKNSEIQDFASQIHQENDKQVLQLMKMVLATN
                     SDLQAAVLVTKDGRTVSTNPQLIMKTSSDMMAEPWYKAAIDRQAMPILTPARQLSLSS
                     KKEWVVSVTQEVVDAAGHNLGVIRLDIAYPTIKASLDQLQLGRQGFAFIVNDKHEFVY
                     HPKKSVYSSSKEMAAMKPYLAIQNGYTKDKTSFVYQKLIPNSQWTLVGVASLDQLHRV
                     QRQIFWSFLGTGLLACLICGFATVLVLRRWIRPIQQLQQVILAIQKGDRQLRAQETGS
                     PELTDLAQQFNALLDQIDSLMVAVADKEKAIGQYRLQALASQINPHFLYNTLDTIIWM
                     AEFNDSKRVVEVTKSLAKYFRLALNQGNEYIRLADELDHVSQYLFIQKQRYGDKLSYE
                     VQGLDVYADFVIPKLILQPLVENAIYHGIKEVDRKGMIKVTVSDTAQHLVLTVWDNGK
                     GIEDSSLTNSQSLLTRGGVGLKNVDQRLKLHYGEGYHMTIHSQSDQFTEIQLSLPKMH
                     ELMADDTQENE"
     gene            14090..15169
                     /locus_tag="VGT87_000555"
     CDS             14090..15169
                     /locus_tag="VGT87_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEQ3813437.1"
                     /translation="MSLATDYFSRQTPIVEKLVAYGFEKRDNGYFYNERFMEGEFEAQ
                     LRIDEAGNIWDRVIDCDLEEDYLPLQQAAWQGTYTGQVRAAYLELLERLSVACFEVTP
                     FQSMQANRLAKHITKEWSDPMDYPFEKHPDLATYRVGGKWYAMIFSLLADKLDQIPER
                     LVGQTCEVMTVKVNPKDLPQLLQQEGIYPAYHMSKNNWVSVVLDDKVTDDQLWGLATQ
                     SRQLVNPNGLSNPNSPDYWVIPANLKYYDIDAEFAANDVILWTQKAGIAVGDYVLIYI
                     TAPVKSIRYACQVLETDIPNEGYRKNLNIDKLMRLRKCQQYKDGLLSFDLMKEHGVAA
                     VRGPRRLSPQLIAFLKEKEYFKENN"
     gene            15183..15899
                     /locus_tag="VGT87_000556"
     CDS             15183..15899
                     /locus_tag="VGT87_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HEQ3813438.1"
                     /translation="MVEFVADQSFWELFPDAKLGVVLLKDVQNQAESPEDVKQLLADS
                     HELAKAYLTADNFSDNQVVQVYRKAYQHFKTKKGARSSIEALLKRVSNGQSIPSINPL
                     VDIYNAASLRFGLPVGAEDSDSFIGDLRLTITDGGDDFYLIGDADNNPTLPNELCYKD
                     DIGAVCRCLNWRDGERTMVTEHTKNAFLIIEALDQEGQNRLQEALDFIEGSAKMYLHA
                     TPSLHVLDKDNPHVPLFTEN"
     gene            complement(16066..16539)
                     /locus_tag="VGT87_000557"
     CDS             complement(16066..16539)
                     /locus_tag="VGT87_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEQ3813439.1"
                     /translation="MNKKETRHQLIRSLISETTIHTQQELQERLQKNGITITQATLSR
                     DMKELNLVKVTSGNDTHYEALAISQTRWEHRLRFYMEDALVMLKIVQHQIILKTLPGL
                     AQSFGSILDAMQIPEIVATVCGDDTCLIVCEDNEQAKACYETLSHYTPPFFFSNK"
     gene            16681..17361
                     /locus_tag="VGT87_000558"
     CDS             16681..17361
                     /locus_tag="VGT87_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEQ3813440.1"
                     /translation="MIRREDYQYLRKLNDFRYFSIEQFDKIVGQMEFRKAKKDHILFF
                     EGDKRDKLFLVTSGYFKVEQSDQSGTFMYTDFIRHGTIFPYGGLFTDDYYHFSVVAMT
                     DVTYFYFPVDLFEDYSLENRLQMKHLYSKMSKLLELHELRVRNLITSSASSRVIQSLA
                     ILLVEMGKDSDTLPFQLTTTDIAQISGTTRETVSHVLRDLKKQELITIKGKYLTYLDK
                     DYFLQYTK"
     gene            17635..18870
                     /gene="arcA"
                     /locus_tag="VGT87_000559"
     CDS             17635..18870
                     /gene="arcA"
                     /locus_tag="VGT87_000559"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEQ3813441.1"
                     /translation="MTAQTPIHVYSEIGKLKKVLLHRPGKEIENLMPDYLERLLFDDI
                     PFLEDAQKEHDAFAQALRDEGIEVLYLETLAAESLVTPEIREAFIDEYLSEANIRGRA
                     TKKAIRELLMAIEDNQELIEKTMAGVQKSELPEIPASEKGLTDLVESNYPFAIDPMPN
                     LYFTRDPFATIGTGVSLNHMFSETRNRETLYGKYIFTHHPIYGGGKVPMVYDRNETTR
                     IEGGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKQNLGFKKVLAFEFANNRKFMHL
                     DTVFTMVDYDKFTIHPEIEGDLRVYSVTYDNEELHIVEEKGDLAELLAANLGVEKVDL
                     IRCGGDNLVAAGREQWNDGSNTLTIAPGVVVVYNRNTITNAILESKGLKLIKIHGSEL
                     VRGRGGPRCMSMPFEREDI"
     gene            18970..19410
                     /locus_tag="VGT87_000560"
     CDS             18970..19410
                     /locus_tag="VGT87_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ3813442.1"
                     /translation="MTDKFDANDETRTVYAVVYDNDQPVSTGQFLAETKIEARLTRIV
                     TLADYCGCGYGAKVTEALETYTRREGFYQLTIHSELTAQTFYENLGYQTYGSKYLEDG
                     EYCQSLVKTILKWEKNMDIAMLIAIVGGLLGCYLYLTKNNEPKD"
     gene            19435..20448
                     /gene="argF"
                     /locus_tag="VGT87_000561"
     CDS             19435..20448
                     /gene="argF"
                     /locus_tag="VGT87_000561"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEQ3813443.1"
                     /translation="MTQVFQGRSFLAEKDFTRAELEYLIDFSAHLKDLKKRGVPHHYL
                     EGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRMF
                     DGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVKENFGKLEGLTLVY
                     CGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEEEIVTLAEGYAKESGARILITEDA
                     DEAVKGADVLYTDVWVSMGEEDKFKERVELLQPYQVNMDLVQKAGNDKLIFLHCLPAF
                     HDTNTVYGKDVAEKFGVKEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLFI
                     PKV"
     gene            20618..22111
                     /locus_tag="VGT87_000562"
     CDS             20618..22111
                     /locus_tag="VGT87_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEQ3813444.1"
                     /translation="MTEEKKRGFRIPSSYTVLFIIIAIMAVLTWFIPAGAYETAKGGG
                     VISGTYKTVASNPQGFFDILMAPVRGMLGVEGTDGAIQVSFFILMVGGFLGVVNKTGA
                     LDTGIASVVRKNKGREKMLIAILIPLFALGGTTYGMGEETMAFYPLLIPVMIAVGFDS
                     IVAVAIILIGSQIGCLASTINPFATGVAADAAGVSIADGMIWRVIQWVILVGMSIWFV
                     YNYASKIEEDPSKSLVADKEEEHKELFQLQNSGEDLNKRQRNVLTIFTLTFVIMILSL
                     IPWEDFGIKFFTNINTWLTTMPILGGVIGKTMGAFGTWYFPEITMLFIMMGVLVAIVY
                     RMSEEDFFSSFLTGAGEFLGVAMICAIARGIQVIMNGGMITATILHLGETSLSGLSSQ
                     VFVILAYIFYLPMSFLIPSTSGLAGATMGIMAPLGQFSNVPAHLVITAFQSASGILNM
                     ISPTSAIVMGALALGRVDLGTWWKFIGKFIVMVMLVSVLLLVVATFF"
     gene            22128..23459
                     /locus_tag="VGT87_000563"
     CDS             22128..23459
                     /locus_tag="VGT87_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEQ3813445.1"
                     /translation="MESYITPKHQEACVAAIKQIVSYPSVCHEGENGTPFGQAIQDVL
                     EATLDLCQGLGFHTYIDPEGYYGYAELGDQKEVLAILCHLDVVPEGDRQLWKTDPFDC
                     VEADGCLFGRGTQDDKGPTMMALFATKALIDAGVTFNKRIRFIFGTDEETLWRCMNRY
                     NDVEEQATFGFAPDSSFPLTYAEKGLLQAKLVGKGCDKLSLEIGQAYNVVPARASYKG
                     NKLEALQKELDKLGFEYVVKEGELTVYGLAQHAKDAPDGINAMIRLAKALVVLEPNKT
                     LDFLANVIDEDGRALNIFGRIEDEPSGKLSFNAAGLTLTKDKAEIRLDIRIPVLADKE
                     KLVQQLSQKAQEYGLTYEEFDYLAPLYVPLDSELVTTLLSVYRKKTGDQSPAQSSGGA
                     TFARTMKNCVAFGALFPDAVQTEHQENEHIVLADAYRAMDIYAEAIYRLTR"
     gene            23479..24429
                     /gene="arcC"
                     /locus_tag="VGT87_000564"
     CDS             23479..24429
                     /gene="arcC"
                     /locus_tag="VGT87_000564"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEQ3813446.1"
                     /translation="MTKQKIVVALGGNAILSTDASAKAQQEALMSTSKSLVKLIKEGH
                     EVIVTHGNGPQVGNLLLQQAAADSEKNPAMPLDTCVAMTEGSIGFWLVNALDNELQAQ
                     GIQKEVAAVVTQVIVDAKDPAFENPTKPIGPFLTEEDAKKQMAESGASFKEDAGRGWR
                     KVVPSPKPVGIKEANVIRSLVDSGVVVVSAGGGGVPVVEDATSKTLTGVEAVIDKDFA
                     SQTLSELVDADLFIVLTGVDNVYVNFNKPDQTKLEEVTVSQMKEYITQDQFAPGSMLP
                     KVEAAIAFVENKPNAKAIITSLENIDNVLSANAGTQIIAG"
     gene            24742..25734
                     /locus_tag="VGT87_000565"
     CDS             24742..25734
                     /locus_tag="VGT87_000565"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEQ3813447.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLIAKLDVVEVQGPILSRVGDGM
                     QDNLSGTENPVSVNVLKIPNATFEVVHSLAKWKRHTLARFGFNEGEGLVVNMKALRPD
                     EDSLDQTHSVYVDQWDWEKVIPDGKRNLAYLKETVETIYKVIRLTELAVEARYDIEAV
                     LPKKITFIHTEELVAKYPDLTPKERENAITKEFGAVFLIGIGGVLPDGKPHDGRAPDY
                     DDWTTETENGYHGLNGDILVWNDQLGSAFELSSMGIRVDEEALKRQVEMTGDQDRLAF
                     DWHKSLLNGLFPLTIGGGIGQSRMVMFLLRKKHIGEVQTSVWPQEVRDSYDNIL"
     gene            25857..26396
                     /gene="rsmD"
                     /locus_tag="VGT87_000566"
     CDS             25857..26396
                     /gene="rsmD"
                     /locus_tag="VGT87_000566"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEQ3813448.1"
                     /translation="MRVVSGEFGGRPLKTLDGKITRPTSDKVRGAIFNMIGPYFNGGR
                     VLDLFAGSGGLAIEAVSRGMSAAVLVEKNRKAQAIIQDNIIMTKAENRFTLLKMEAER
                     AIDCLTGRFDLVFLDPPYAKETIVATIEALAAKNLLSEQVMVVCETDKTVLLPKEIAT
                     LGIWKEKIYGISKVTVYVN"
     gene            26386..26877
                     /gene="coaD"
                     /locus_tag="VGT87_000567"
     CDS             26386..26877
                     /gene="coaD"
                     /locus_tag="VGT87_000567"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020905327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEQ3813449.1"
                     /translation="MLTKIGLYTGSFDPVTNGHLDIVKRASGLFDQIYVGIFDNPTKK
                     SYFKLEVRKAMLTQALADFTNVIVVTSHERLAIDVAKELRVTHLIRGLRNATDFEYEE
                     NLEYFNHLLAPNIETVYLISRNKWQALSSSRVRELIHFQSSLEGLVPQSVIAQVEKMN
                     EKT"
     gene            26864..>27779
                     /locus_tag="VGT87_000568"
     CDS             26864..>27779
                     /locus_tag="VGT87_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEQ3813450.1"
                     /translation="MKRLKKIKWWLVGLLALISLLLALFFPLPYYIEMPGGAYDIRTV
                     LQVNGKEDKRKGAYQFVAVGISRASLAQLLYAWLTPFTEISTAEDTTGGYSDADFLRI
                     NQFYMETSQNAAIYQALSLAGKPVTLDYKGVYVLDVNNESTFKGTLHLADTVTGVNGK
                     QFTSSAELIDYVSHLKLGDEVTVQFTSDNKPKKGVGRIIKLKNGKNGIGIALTDHTSV
                     NSEDTVIFSTKGVGGPSAGLMFTLDIYDQITKEDLRKGRTIAGTGTIGKDGEVGDIGG
                     AGLKVVAAAEAGADIFFVPNNPVDKEIKK"
BASE COUNT         8639 a         4874 c         5948 g         8318 t
ORIGIN      
        1 ccaaatacga cgcaacccaa gaacggattg cagagatgaa gaaaattaaa tcatagtgtt
       61 tagaacgcag ttgaaaaact gtgttctttt cttactaata gagatattag attgaagttt
      121 aattatgaga aaaacgcttt ctttatttaa agagttatgt tacaatacta ttacaatcat
      181 cgtacaaata gtgttatttt gaggggcata tgagaatgat tttagcacac tatgactgta
      241 aaaaagataa aaagcaatct ttagatgagc atttatggca tgtggcctgt tctagtcgac
      301 aggaagcatc tataattggt caaggagatg tgcttttttt aattggtctt taccacgacc
      361 tgggcaaagc tgatcgaacc tttcaagata aattattaaa taatccaaat cggcatgttg
      421 atcactctta tgcaggggca aaatacttat gttctattat tgggcctcat ctaaaaaacc
      481 gaggggttga taaaaatgag agaatgacat tcaacgaaat ggtggggtat gtcatctctg
      541 ctcatcatgg gatgtatgat ttatgctact attttgacga tgctgaatat tatggcttta
      601 ataagtttaa aaatcgtatc aatagagact tagatggtta tcactatcat gaagatatta
      661 aagggtacgc tctaaaatta gaaaaaaatt atgtgattat ggctacaaag atttaaggga
      721 gcttattgat aaagcttttg ataattacca acaatccatg tcttccttaa actggcaaga
      781 taagagtgag tgggattatt atcagtcttg tatggtgaga ctttacttgt cactcttaaa
      841 aaacgctgat attttggaca cagtaaatgt ctatggcctt aagataagtc ctatggataa
      901 aacagagcga tcctttctaa aacactccta tttagcggcc attgaacaaa aatatgctag
      961 ctttggacag ccaaacaatc agttgaacac tattcggaca gaaatcgctg agcgtgttaa
     1021 agaaagaggt aaacgagatt ccaaggggat ttatcgctta gatttaccga caggagctgg
     1081 caagactaat cttagtatgc gttatgcgtt tcaccaatta gttcatcacg acaaatcaag
     1141 gtttttttac ataacgccct ttctttcggt tcttgagcaa aatgcttccg aaattagaaa
     1201 agttacaggt gaccttggcg ttctagaaca ccattccaat gtggtgaaac aggctaatga
     1261 agatgatgat gacaaggaca gtttattgtc agcttatctt agtgatagct gggacagtca
     1321 agtagtcttg acttctatgg ttcaattttt ccaaacactt ttcaaaacaa aatcagctaa
     1381 tctgagacgt ttttcaagtt tgattaatag tgttgtgatt ctagatgaag ttcaatccct
     1441 gcctattgaa gtcaccactt tgtttaattt aacgatgaat tttttaaata aagttatgga
     1501 tacaaccatc gttctttgca cagcaacaca acctgcttat gattcttcag agattgacca
     1561 tcgtatctgt tatggaggga acttgggaga attagctgaa atcgttgagt taacgattga
     1621 agaaaaacag attttttcaa ggacagagct tagaaaattt gatgatagtg atcagaaagt
     1681 tcacttgact gatgttatta accttattct aggtgaggaa aactcagttc ttgctatttt
     1741 taatacgaaa aaaacggttc ataactgcta tactatgcta aaagacatga ctgatagacc
     1801 ggtctatcag ctttcgacaa atatgtgtgc gcagcataga cttgacttga ttgctaagat
     1861 caaaacggag ttacaaaata atatccctat tatttgtatt agcacgcaat taattgaagc
     1921 aggtgtagat gttgattttc atcgcgtcat tcgttcctac tcagggattg attctattat
     1981 tcaggctgct ggacggtgta accgagaagg caaacgagat aaagggcaag tcactcttgt
     2041 caatctgacc aatgaagagg aaaatatttc taggctgaca gaaataaaag ctaaaaaaga
     2101 agccacagaa tctattcttc ataagattgg gtctccaatt gatatcttaa ctttaaaccg
     2161 tgactttttt gagtattatt atgccaataa tcagggactg atggattatc ctttggaaga
     2221 caacctatca atctacgact atttaagcct taatatttat cagacggcaa ataaaaagtt
     2281 caaaggtaag ttaaaacaag cttttaaaac agcaggagcc aaaatgaacc tcatcaataa
     2341 tgatatgata ggaattctcg taccttatgg cgaagctgag aaaaaattgg cttatttaga
     2401 agaattgggt atgtctcact ttttatcagc agaagattat caaacgataa aatcattact
     2461 aaaagagtta caacctttta cggttaatgt ccgcgagaac gatcctctct ttgagacaac
     2521 aaaatcttat ctaaatggtc agattctggt tttgacgtcg gagtattatg acacggaaag
     2581 aggagttaaa tacgattcag ctagctttta cttctaactc aaaacgaaag cagattaaca
     2641 aaaggttgtt agaggacctt gttaacctgc caatcatcat taataattat tatcaattta
     2701 gactatttaa taaaattaga ttacaaaaaa acagaaggag gaaagtagct tgtacagatc
     2761 tagagacttc tacgtgagag taagtggtca gcgagctctt tttacaaatc cagccacaaa
     2821 agggggatcg gaacggtcat cctattcggt tccgactaga caggcactga atggtatcgt
     2881 tgatgccacc tattataagc cgacctttac taatatcgtc acagaggtta aggttattaa
     2941 ccagattcaa accgaattac agggtgtcag ggctctgtta catgattata gtgcagattt
     3001 aagttatgta tcctatttga gtgatgttgt ttatctgatc aagtttcatt ttgtttggaa
     3061 tgaagataga aaagatttga actcagatag acttccagct aaacatgaag ccattatgga
     3121 gcgttctatt cgtaaagggg gacgtcgaga tgtgtttttg ggtacaagag aatgtttagg
     3181 gcttgtagat gatatcagcc aagaagagta tgagactact gtgtcgtatt ataatggtgt
     3241 caatatcgac ttgggaatca tgttccattc ctttgcctat ccgaaggaca aaaagacacc
     3301 attaaaatca tactttacaa agactgtgat gaaaaatgga gtcattacgt ttaaagcaca
     3361 gtctgaatgc gatattgtta acacgctttc tagttatgct tttaaagcac cagaggagat
     3421 aaaatcggtt aacgatgaat gcatggagta tgatgccatg gagaaaggag aaaactgatg
     3481 gattttttta cttctctctt gaagacttat gaaaaagcag agctagcaga cttggttgat
     3541 catcaaaaaa gaaataatga gccggtttta ctgccgattt atcatacgag tttaaagtca
     3601 aatggtaaaa atatcatttc agtgaaactt gacaaagatg gccagtttca caaggcagaa
     3661 tttatggtag ataagcaaat gattattttt cctgtaacgg ctgattctgt tgctaggtca
     3721 ggtagtcatc ctgcaccgca tcctctagtc gataaatttg cttattatag tgctgaaatg
     3781 gggcagtccc agtatgattc ttttcataag caactgaata actggattga ttattgtgag
     3841 gagggtgacg tcaagaggtt tttaaccatt gttcagcaat ttattttgaa accagaattt
     3901 ctgacactga ttctcgattc tttaattggt cctgattatc aacataatca attaaaagtc
     3961 acattttgtg atgccactgg aaaagaaaaa ttaattgatt tatcagcttg ctttttagaa
     4021 ttttcaattg atcagttcca gggctttaaa aatgaatcgg tttcgacgtt taaagcttta
     4081 caccaatcct atatttcttt tgttgaagcc aatcgtgaaa atctcggtat ttgtaatatt
     4141 agtggacgag aggaacagct taccgataag catagaggtt tgatggggaa tgctaaaatc
     4201 atctctgtta gtaataaaag agaagcttat aaaggacgtt ttagagaacg cgaagacgtt
     4261 tttagtgttg gctatgaaac ttccgaaaag attcatttaa tgctcaagta ccttttagaa
     4321 aataaaaata ccattacttg gttagggtct tctcaatatt taatcaactg gtttagtgat
     4381 gatttaacaa atgatagtcg gttggatatc gtatcaccaa tctttgatga cggacttgaa
     4441 gaagatgatg atgacgatac gcctcctgtt ataacattag caactgaaga caataaaaga
     4501 attggtaaat cattcatcaa gggacaaaaa ttatttgcta atgatgccac ttactacgtt
     4561 gctattttga ataaaaccag caatgggcgg attgctttaa aatattttcg tcagcttcaa
     4621 gcgtcccaat tactcaccaa tcttaacaag tggcaggaaa catacagttg ggagtcgcga
     4681 tctaagtttg ggaaaagtcg cttaagaacc cctactgttc atgacatcct taatgtgtcc
     4741 tacggggttg atagggatcg cttccttgaa ttagataatg ataacttcaa aagtgatcaa
     4801 attcaaaagt tagtggcaag tttgattgat ggtaaaccga tgccacagtc cattgtcaaa
     4861 aagttaggta acaatgttaa agaacgacat cgttaccgta agcactggta tcaagttgag
     4921 caggtctgct tagcaatttt acacaaacaa aatggggagg aattttcacc gatgctagat
     4981 cataccaatc aaaatcgttc ctatcttttt ggacgattat tagcaatttt tgaattaatc
     5041 gagaccttgc gttatggctt ggatggaaac aataacgacc gtattaccaa tgctgaacgt
     5101 tattggacag cctatactgg acaaccaaca aaattgatga tgttattgga aaataaaatt
     5161 aagccttacg aagaaccatt gaaattaaat cgtcgtggca gttggatgaa attagaaaaa
     5221 gaaaaagaag agattttaga actgttaaat cctctgttag aaacagaaac aatggaaaaa
     5281 cccttagatt accgctttat ttttgggtat tatgctgaga aaaactatta ctatacaaaa
     5341 caaaacacgg aagtaacaga aagtgaggag taaaaagatg ttggaacaca aaattgattt
     5401 tatggtaact cttgaagtga aagaagcaaa tgcaaatggt gatcccttaa atggaaacat
     5461 gcctcgtaca gatgccaaag gatatggtgt gatgagtgat gtctccatta aacgtaagat
     5521 tcgtaatcgt ttgcaagata tggggaagtc tatttttgtg caagctaatg agcgtattga
     5581 agatgatttt cgttcactgg aaaaacgctt ttcgcaacat tttacagcta agacacctga
     5641 caaagaaatt gaagaaaaag caaatgcatt atggtttgat gttcgtgctt ttggacaagt
     5701 ttttacttat ctgaaaaaat caattggggt gcgtggacca gtttccatca gtatggctaa
     5761 gtccttggag ccaattgtca tttccagcct tcaaattacg cgtagtacca atggtatgga
     5821 agctaagaat aatagtggcc gctcttctga tacgatgggg acaaaacatt ttgtagatta
     5881 tggtgtgtat gtacttaaag gttctatcaa tgcttatttt gctgaaaaga ctggtttttc
     5941 tcaggaagat gctgaggcta ttaaagaagt tttggttagc ttgtttgaaa atgatgcgtc
     6001 gtctgcacgt ccggaaggct ctatgcgagt ttgtgaagtc ttttggttta cgcattcaag
     6061 caaattggga aatgtttcaa gtgcgcgtgt ctttgacttg ttagagtata atcaatcaat
     6121 agaagaaaaa agcacttatg acgcttatca gattcatcta aatcaagaaa aattggctaa
     6181 atatgaagcg aaagggttaa cggttgaaat cctagaagga ctctagtatg gtctatgccg
     6241 aagatgatta tttaatgcta tcaggtattc agcatttcca attttgtaaa cgtcaatggg
     6301 cgttgatcca tattgagcaa caatggcttg ataatgaagc gacagcgcat ggacaggttt
     6361 tacatactaa agcagataac ccttacatta aagaaaaacg aaaagagctt ttggtctcac
     6421 gtgctatgcc catttcttct gcagaacttg gactttcagg aattatggat gttgtggaat
     6481 tttataaaga tgatcaaggt gtgtctttga ggggaaaacg tgggaaatgg ttaccaaaag
     6541 ttgtggaata caagcgcgga aaacctaaaa aagataccag agatattgtc cagttggtgg
     6601 ctcagaccat gtgtttagaa gaaacgctag actgcgacat taacgaaggt tgtctttatt
     6661 accatagtgt caatcaaaga gtgattgttc ctatgacatc agctttgcgt caagaagtga
     6721 aggaattagc cgcagagatg catgaggttt atcagagtca aatgctacct aaagcagctt
     6781 attttaaaaa ctgtcagctt tgttctttag tcgatatttg taagcccagg ttgagtaaaa
     6841 aaacaaggag tgtgtcgcgt tacatcaatg aggctatgac cagtgaggag atggacctat
     6901 gaagaagttg ctaaatacct tgtatttgac gcaagaagat ttttatgtca ctaaagaggg
     6961 cgataacatt gttatcaagc aagaaggtaa ggttctcaaa cggtttccgt ttcggattat
     7021 tgacggtatt gtctgttttt cttatttggg tgtgtcgcct gctttggtga agttatgtac
     7081 ggagaatcag attaaattat cgtttcatac accacaaggg cgtttttgtg gtcgctatat
     7141 tggttcaacc aatgggaatg tgttgttgcg tagagaacat tatcgtttat ctgatcgtga
     7201 ggaatctttg gaatacgcaa agcggtttat tttggctaaa atttccaact caaggaaata
     7261 cttgctacgc tttaaacgag atcatcgtca acagattgat accaagcttt ttgaggctgt
     7321 taatgacgaa ttgatatggg ctttagagat ggttcaggca gcagataata aagactcttt
     7381 aagagggatt gaaggccaag ctgctaatca gtattttcgc atatttaatg acctggtgtt
     7441 gacggacaaa aaaacgtttt acttccaagg tcggagtaaa cgaccaccct tagattgtgt
     7501 taatgccctc ttgtcttttg gttacagttt actgaccttt gaatgtcaat ctgccttgga
     7561 agctgtcgga ttagacagtt acgttggttt ctttcacacg gatcgtcctg ggcgtgctag
     7621 tttagcgctt gatttagttg aagagttccg ctcatatatt gtagatcgtt ttgtcttttc
     7681 attaattaat aaaggacaac ttcagaaaaa acactttgag gttaaagaaa atggtagtat
     7741 tttattgacg gaaaatggca gagctatttt tattgatttg tggcagaagc gtaagcatac
     7801 tgaggtagaa catcctttta caaaagagaa agtaaaactt atgttattac cctatgtaca
     7861 agcgcagctt ttagctaagg ccatacgagg agatttagaa agctatccac cttttatggt
     7921 ttaggagatg ttatatgatg gttttagtca cttatgatgt aaatacggaa acacctgctg
     7981 gtagaaaaag attgcgtcat gttgccaaac tctgtgtgga ctatgggcaa cgtgttcaaa
     8041 attctgtttt tgaatgttct gtgacacccg cagaatttgt ggatataaag caccgcttaa
     8101 cacaaatcat tgatgagaaa actgatagta ttcgctttta tttattgggg aaaaattggc
     8161 agaggcgtgt ggaaacactt ggtcgctcag acagctatga cccagataaa ggtgtcttat
     8221 tattgtaaaa atctcttgtg cgaagctagc tttcacagaa aaaccttgct tgctcgcgca
     8281 aaaataactt aaaaaagaag cgaaatggag ataaaaaggc ttaaaacacc tctatgtgtg
     8341 ccatccgttt ctctataaac tgtgtcattt ggcgcagtct cacccttcat gggtgagtgg
     8401 attgaaatta aaagtaggag gaactttagt ttttaaatgg tcgtctcacc cttcatgggt
     8461 gagtggattg aaatagccaa tttgtcagca acatcatcct ttgatttgtc tcacccttca
     8521 tgggtgagtg gattgaaata gttatgggac ctaaccagca atccatgctt aaagtctcac
     8581 ccttcatggg tgagtggatt gaaattgcta ataacggctc tgcaagtggg gcatgggcaa
     8641 gtctcaccct tcatgggtga gtggattgaa atatgattac acgcaaaatg aacacaagga
     8701 taacgagtct cacccttcat gggtgagtgg attgaaattg ctacggcgta tttagtcaca
     8761 tactcgcgta cagtctcacc cttcatgggt gagtggattg aaagatgaac ccgatctctc
     8821 ttttaatgag gtcgggtttt tacatgcctt tagtcaacca tttatctgat agcgtatcaa
     8881 attcaagttt ttgcatgttc tagtctatgg tttgcgccac tagctttcgg taaactaagg
     8941 gtaacttaag agaggagtta tttatgacac tatcacttgt attaatggta tcggtttttg
     9001 gggcaggctt gttatccttt ttctctccgt gtatctttcc ggtccttcct gtctatttag
     9061 gcattttact ggatgcagat gattcaaaga cgattactat atttggtaaa aaactctatt
     9121 ggtatggcat tgtcaaaact ttagccttta tttttggtct atctactatt tttgtgattt
     9181 taggttacgg ggctggtttt ttaggaaata tcctttatgc ggtatggttt cggtacttac
     9241 tgggagcctt ggtcattatc ttggggattc atcagatggg cctcattacc attaagagct
     9301 tacaatttca aaaatcactg actttccata ataacaagaa tcgcaacggt ttgttcaatg
     9361 catttatcct tggtttaacc tttagctttg gttggacgcc ttgtgtggga cctgttttga
     9421 gttctgttct agctttggtg gcttcagggg gaaatggtgc ctggcaaggt ggcgttttga
     9481 tgattattta tactcttgga ttgggcattc ctttcctgct tatctctttt gcgtcaggca
     9541 ttgttttgaa acagtttaac aagctcaaac cccacatgct tttactgaaa aaagtaggag
     9601 gcgttctgat tatagtcatg ggaatcttgc ttatgacagg aaccttaaat aacttagcac
     9661 aactttttgg ataaaggaga aacacaatga aaaaaggact attagtaaca actggtttgg
     9721 cttgtctcgg gctactaact gcttgctcaa cccaagacaa tatggctaaa aaggaaataa
     9781 ctcaggacaa gatgagcatg gcagctaaaa agaaagataa gatgtcaaca tcaaaggaca
     9841 agtccatgat ggcagataaa tcatctgata agaaaatgac caatgatggt cctatggcgc
     9901 ctgatttcga actcaaaggc attgatggta agacctatcg cttatcagag ttcaaaggta
     9961 aaaaagttta tttgaaattt tgggcctctt ggtgttcaat ctgtctatca accttggcag
    10021 ataccgaaga cctggctaaa atgtcagata aagactatgt ggtcctaaca gttgtctcgc
    10081 caggtcatca aggggaaaaa tcagaagcag actttaaaaa atggttccaa ggaacagact
    10141 ataaggactt accagtcttg ttagatccgg atggcaagtt attggaagct tacggcgtca
    10201 gatcttaccc aacagaagtt ttcattggaa gtgatggtgt tcttgctaaa aaatacattg
    10261 gttatgccaa aaaatcagac atcaaaaaga cccttaaagg tatacattag gaaggaccag
    10321 ctgataggta ttagaaaaaa ggagttgagt ttatggaaca caaatggaaa ttatggctat
    10381 ttatcgttgg aatggtcttg ttaataggcg gagcttctgt tctcattcag aaaggtcaag
    10441 ccaaggcttt tagtcaaggc aagcctcaac taccagctaa aaaagtcaaa tccactaaag
    10501 cagatcccaa tgcccctgtt gatcaaaaaa tgatttacct agctggtggc tgtttctggg
    10561 gcgttgaaga atatttttca caagtggatg gcgtcttaga tgcggtgtca ggatatgcca
    10621 atggtagagg cgataccact aattaccagt taattcatca gactggtcat gcagaaaccg
    10681 ttgaagtggc ctatgatgcg aaccgtatta gcttgaaaga attattactg cattttttcc
    10741 gaattattga cccaaccagt cttaataagc aaggaaatga tcgcggtagt caataccgaa
    10801 ctgggattta ctatactgac aaagcagatt tagctattat tgatgaggtc ttcaaagaaa
    10861 aagctaagga ttacaagaaa aaaatagtgg ttgaaaaggc gccattgaaa cactttatta
    10921 aggccgaaga gtaccaccaa gattacctca agaaaaatcc aaatggctac tgccatattg
    10981 acatcaacca agcgacctat cctgtgattg atgaaagcaa gtaccccaaa ccaagtgcca
    11041 ctgagatcaa agcaaaacta tctgcagacg aataccgagt gacgcaaaaa aacgaaactg
    11101 aaaaagcttt ttctaatcgc tactgggatt cttttgatgc aggaatttac gtagacgttg
    11161 tgacaggaga accgcttttc tcttcaaaag acaaatttga atcaggttgt ggttggccaa
    11221 gctttagccg ccccatcagc ccagacgttg tccgctacaa ggaagataag agtttcaata
    11281 tgacgagaac agaagtgcgc agccgttcag ggaattctca tctggggcat gtctttactg
    11341 acggacctaa agaccaaggc ggccttcgtt actgcatcaa tagcttgtct attaccttta
    11401 ttccaaaagc tgatatggaa gctaagggct atggttattt attatcatct gtagaatagg
    11461 gttaggccta aaaatctgat ataatagaag aaagactagt atgagagggc catgcaaggc
    11521 cctttataaa gataaggaga caccgtgtgt actcattgtt aattgtagaa gacgaatacc
    11581 ttatccgcca aggtattcgt tctttggttg attttagcca gttcaagatt gatcgggtca
    11641 acgaagcaga aaatggccag ttggcttggg acttgtttca gaaagagcct tatgatatta
    11701 ttttgacgga tatcaatatg cccaaattaa atgggattca actagcagaa ctcattaaac
    11761 aggaatcccc ccaaactcat ctggtatttt tgacgggcta cgatgatttt aactatgcct
    11821 tatctgcttt gaaattaggg gcagatgatt acttgctcaa acccttttcc aaggcagatg
    11881 tagaagacat gttaggaaag ctccagcaaa aattagaact ttccaagaaa acagaaacca
    11941 ttcaggaatt ggttgagcag cctcaaaaag aagtatcagc aatagcaatg gctattcatg
    12001 agcggttggc agattctgat ttgaccctaa aaagcttggc tcagcagctt ggttttagcc
    12061 ctaattacct tagcgtcttg attaaaaaag agttagggat gccctttcaa gattatctgg
    12121 tacaagaacg gttgaaaaaa gccaagcttc tcttgttaac cagtaacctt aaaatctatg
    12181 aaattgcaga acaggtcggt tttgaggaca tgaattattt ttctcagcgc tttaagcaac
    12241 tggtaggcgt taccccaagt cagtataaaa aaggaggcca ggcatgaagc gttatcccct
    12301 actggtccag ttgatttctt atgtctttgt gatcgtaatt gccctcatta ccacgcttgg
    12361 tttgctttat taccagacga gttctcgtaa tatcaggcaa ttaattgaac gtgatacccg
    12421 gcagagcatt cggcaaagtt cccaatttat tgacgcttac atcaagcctc ttaaagaaac
    12481 aacttcggtg ctggcgaaaa attcggaaat tcaagacttt gctagtcaaa ttcatcaaga
    12541 aaatgacaaa caggttcttc agctcatgaa gatggttctt gcgaccaatt ccgatttaca
    12601 agcagctgtt ctggtgacta aggatggccg aaccgtttct accaatcctc agttgattat
    12661 gaaaacctcc agtgatatga tggcagaacc ctggtataaa gcagccatag accgtcaagc
    12721 catgccaatc ttaaccccag ctcggcaatt atccctttct tctaaaaaag aatgggtagt
    12781 ttctgtgacc caagaggtag tggatgctgc tggtcataat ttaggcgtga taagacttga
    12841 tattgcatat ccgactatta aagcgtcttt agatcagctt cagctaggcc gccaaggctt
    12901 tgcctttatt gtgaatgata agcatgaatt tgtttaccat cccaaaaaga gtgtttacag
    12961 ttcttctaag gagatggctg cgatgaagcc ttatttagcg attcagaatg gttacactaa
    13021 ggacaagaca tcttttgttt accaaaaact cattcctaac agtcaatgga ctttagtggg
    13081 agtggcgtca ctggatcagt tgcaccgggt gcagcgccaa attttttggt cctttcttgg
    13141 aacagggctt ctagcctgtc tgatttgtgg ctttgcaact gtcttagtct tacgcagatg
    13201 gattcgtccc attcaacaat tgcagcaagt tattcttgct attcaaaaag gagatcgtca
    13261 gttacgtgcc caagaaacgg gttctccaga attgacagac cttgcccaac agtttaatgc
    13321 gctcttagat caaattgata gcttgatggt tgccgttgcg gataaggaaa aggcgattgg
    13381 gcagtacagg ttacaagcct tggctagtca gattaacccg cattttctct ataacacctt
    13441 ggacactatt atttggatgg cagaatttaa tgacagcaag cgcgtggtag aagtgaccaa
    13501 gtctctagct aagtattttc gtttggccct taatcagggg aacgaataca ttcgtttggc
    13561 agatgaactg gatcacgtta gccaatacct ttttattcaa aaacagcgct atggagacaa
    13621 gctaagttat gaagtgcaag gcttagatgt ctacgcagac tttgttattc ctaagcttat
    13681 cttacagccc ttagtagaaa atgctatcta ccatggcatc aaagaagtcg atcgcaaagg
    13741 catgatcaag gttacggtat ctgatacagc tcagcatctg gtgttgactg tttgggataa
    13801 tggtaaaggc attgaagact cttcactgac caatagtcag agcttgttga ctaggggagg
    13861 tgtgggcctt aaaaatgttg accagcggtt aaaacttcac tatggtgaag gctaccacat
    13921 gaccattcat agccagtcag accagttcac tgaaatacaa ttaagccttc ctaaaatgca
    13981 tgaattaatg gcagacgaca cacaggaaaa cgagtaaggt tacaggaagt gcatgtcagc
    14041 catttcccta agttagggta ttggagatag gaaccttaga aaggatatca tgagtttagc
    14101 aactgactat tttagccgac agacccccat agttgaaaag ctagtggctt acggttttga
    14161 aaaacgagat aatggttatt tctataacga acggtttatg gaaggagaat ttgaggcgca
    14221 gcttaggatt gatgaggctg gcaatatctg ggatcgggtt attgattgtg atttggagga
    14281 ggattatttg cctttacaac aagcagcctg gcaaggaacc tatactgggc aggttagagc
    14341 agcctatttg gaacttttgg agcgcttatc agtagcctgt tttgaagtca ctccttttca
    14401 gtcaatgcaa gctaatagac ttgctaagca tatcaccaaa gaatggtctg acccaatgga
    14461 ctatcctttt gagaagcacc cagaccttgc aacctaccgg gtagggggca aatggtatgc
    14521 tatgattttt tctctcttgg ctgataaatt agaccagata ccagagagat tagtaggaca
    14581 gacatgtgaa gtgatgactg tcaaagttaa tcccaaggat ttacctcagt tactgcagca
    14641 agagggcatt taccctgcct accatatgtc aaaaaacaat tgggttagtg ttgtcttgga
    14701 tgataaagta acagatgatc agctttgggg gttagctacg caaagtcgtc aactagtcaa
    14761 ccccaacggt ctttctaatc ctaatagtcc tgattattgg gtcattccag ctaatttgaa
    14821 atactatgat attgatgcag aatttgcagc caatgatgtg attttatgga cccaaaaagc
    14881 tggtattgcc gtgggagact acgtgctgat ttatataaca gccccagtca aatccattcg
    14941 ctatgcctgc caagttctag aaactgatat acctaacgag ggttatcgta aaaatcttaa
    15001 cattgacaaa ctcatgcgtc tccgaaaatg ccaacagtac aaggatggtc tcttgtcttt
    15061 tgaccttatg aaagaacatg gcgtagctgc agtgagaggg ccaaggcgat taagccctca
    15121 attaattgca tttttaaagg aaaaagagta ttttaaagag aacaactaaa ggagaataag
    15181 agatggttga atttgtagca gatcaaagtt tttgggaact ttttccagat gccaaactag
    15241 gtgtcgtctt attaaaggat gttcagaatc aagcagagtc accagaagat gtcaaacagc
    15301 tcttagcaga tagccatgag ttagcaaaag cttatttaac agcagacaat tttagtgata
    15361 atcaggtggt tcaagtctac cgcaaggcct atcaacactt taaaaccaaa aaaggagctc
    15421 gctctagcat tgaagccctt ctcaagcggg tgtcaaatgg tcaaagcatt ccgtcgatca
    15481 atccgctagt ggatatttat aatgcggcta gtctacgttt tggtttacct gttggagccg
    15541 aagatagtga cagttttatc ggagatttgc gtctgacgat aacagatggt ggtgacgact
    15601 tttacttgat tggggatgct gataacaacc caaccttgcc aaatgagctg tgttataaag
    15661 atgatattgg agcggtttgt cgttgcctta actggcgaga tggcgaacgc accatggtga
    15721 cagaacacac caaaaatgca tttctgatta tcgaagctct tgatcaagaa ggtcaaaata
    15781 gattgcaaga agctcttgac ttcattgaag gcagcgcaaa aatgtacctt catgcaaccc
    15841 cctctttgca tgtgttggat aaagacaatc cacacgtgcc actttttaca gaaaactaga
    15901 ttagtaggat gagctgtgta aaatagaagg ttaacaaaaa aagcgagtaa acgaatgtcc
    15961 tgtcatttag gttttcgttt actcgctttt tgatgagatt ttactgctgt cctagcaaaa
    16021 ggtgaggatg aggtcgtata tcacatagag gttttctgtt aagccttatt tgttactaaa
    16081 gaagaaaggt ggcgtgtaat gactaagggt ttcgtaacaa gccttggctt gctcattgtc
    16141 ttcacagaca atcaagcaag tatcatcacc acagaccgtt gctacaattt ctggaatttg
    16201 catggcatct aaaatggagc caaaggattg ggctagtcca ggtaacgttt ttaaaataat
    16261 ttgatgttga actatcttta acatgaccaa agcgtcttcc atgtaaaaac gaagacgatg
    16321 ttcccagcgg gtttgtgaaa tggctaacgc ttcatagtgt gtgtcgtttc cagaagttac
    16381 tttgaccagg ttcaattcct tcatgtcacg tgatagggta gcctgagtga tcgtgatgcc
    16441 atttttttgc aaccgttctt gtaattcttg ctgtgtatgt atagttgtct cagagatgag
    16501 ggaacgtatg agttggtgac gcgtctcttt cttattcatt gttataatct tcctttaacg
    16561 taattgataa caatccgagt atagcaaaaa gaggagaagg tgtaaagttt ttgatgaacc
    16621 gcttacaaaa tagctttttt atggtaaaat aaagatgatg tttagaaaaa ggagatttgt
    16681 gtgattcgaa gagaagatta tcaatacctt agaaaactaa acgattttcg ctacttttct
    16741 attgaacaat ttgacaaaat tgtagggcag atggaattcc gaaaagctaa aaaggaccat
    16801 atcctttttt ttgagggaga taaaagagat aagctttttc tagtgacctc tggttatttc
    16861 aaggtggagc agtccgatca atcaggaacc ttcatgtata cggatttcat cagacacggc
    16921 accatttttc cttacggtgg cctctttaca gatgattatt accatttttc agtggtagcc
    16981 atgacagacg tgacttattt ttactttcca gtcgatttat ttgaagatta ttcgcttgaa
    17041 aatcgtctac aaatgaaaca cttatatagt aagatgtcta agctgctcga gttacatgag
    17101 ttgagggtcc gaaacttgat tacatcaagt gctagttctc gtgtgattca atctctggcg
    17161 attttgctgg ttgaaatggg taaagacagt gataccttac cttttcaatt aacaacaaca
    17221 gatattgctc aaataagtgg gacaacaaga gaaacagtta gccatgtgct tagggatttg
    17281 aagaagcaag aattgattac cataaaaggt aagtatttga cttatctaga taaagactat
    17341 tttttacaat acacaaagtg aattaaaggt cagaacactt tattttaaaa gtttttctga
    17401 cttattttaa tttataagta taaattttta cactaagatg taaaaaacat acaagtaaac
    17461 gaatacaaat ctataaaaat aaaaacaacg ctcaaaaaat gtaagcgatt actcaaaatg
    17521 gctgtgagaa aaaccctatt tttatctttt ttaaggagta cactaggaat ataagcaaga
    17581 gttaagaatc ttgacggtga attcacaaaa accaattaga aagaggtaat tcttatgact
    17641 gctcaaacac caattcatgt ttattctgaa attggaaaat taaaaaaagt gttgctccac
    17701 agacccggta aagaaattga aaatcttatg ccagactact tggaacgctt gttgtttgat
    17761 gacattccat tcttagagga tgcccaaaaa gaacatgatg cttttgccca agctttgcgt
    17821 gacgaaggta ttgaagtctt gtaccttgag accttagctg cagaatctct tgtcactcca
    17881 gaaattcgcg aagctttcat cgatgaatac ctaagtgaag ctaatatccg tggaagagct
    17941 actaaaaaag ccattcgtga gctgttgatg gctatcgaag acaatcaaga attgattgaa
    18001 aaaaccatgg caggggttca aaaatctgaa cttccagaga tcccagcatc tgaaaaaggc
    18061 ttgactgact tggttgaatc caattaccca tttgcgatcg acccaatgcc aaacctttat
    18121 ttcacacggg acccattcgc aactatcggt acaggtgttt ctcttaacca catgttctca
    18181 gaaacccgta accgtgaaac cctttatggt aaatacattt tcactcatca cccaatttac
    18241 ggtggtggca aagtgcctat ggtttatgac cgtaatgaaa ccactcgcat tgaaggtggg
    18301 gacgaacttg ttctttcaaa agatgtgctt gcggttggta tttctcaacg tacagatgct
    18361 gcttctattg aaaaattgtt ggttaacatc tttaaacaaa accttggctt caagaaagta
    18421 ttggcctttg aatttgcaaa taaccgtaaa tttatgcact tagacactgt ctttaccatg
    18481 gttgactatg acaaatttac cattcaccca gaaattgaag gcgaccttcg tgtttactct
    18541 gtcacttacg acaatgaaga acttcatatc gttgaagaaa aaggtgattt agcagaactt
    18601 cttgctgcta accttggtgt tgaaaaagtt gaccttatcc gttgtggtgg tgacaactta
    18661 gtagcagcag gtcgtgaaca atggaacgat ggttctaaca cccttactat cgcaccaggt
    18721 gtggttgtgg tttataaccg taacaccatt accaatgcta ttcttgaatc taaaggcttg
    18781 aaattgatca agattcacgg aagtgaattg gttcgcggtc gtggtggacc tcgttgtatg
    18841 tcaatgccat ttgaacgtga agatatttaa taagctatgg taaaggtggt tataggtcag
    18901 aagccttttt aaagggcagc tagtgtttat cttcgattct gtgtctttgt ccttcaatga
    18961 aattttgtca tgacagataa atttgatgcc aatgacgaaa caagaacggt ttatgcagtc
    19021 gtttatgaca atgaccagcc cgtttcaaca ggacaatttt tagctgaaac gaaaatagaa
    19081 gcacgattga cacgcattgt aaccttagca gattattgtg gttgcggtta tggtgccaaa
    19141 gtcactgaag cgctagaaac ttataccaga cgagaaggct tttaccaact aaccattcac
    19201 agtgaactga cagcacaaac cttttatgaa aacctaggtt atcagaccta tggttccaag
    19261 tatttagaag atggtgagta ttgtcaatcc cttgttaaaa ccattcttaa atgggagaag
    19321 aatatggaca tagcaatgct aattgcgatt gttggtggtc tattaggctg ctatctctat
    19381 ctcacaaaaa ataatgaacc caaagattaa gttaatactc gaaggagaca atagatgaca
    19441 caagtatttc aaggacgtag cttcctagca gaaaaagatt ttacacgcgc tgaattagaa
    19501 taccttattg atttttcagc tcatttgaaa gatttgaaaa aacgtggtgt gcctcatcac
    19561 tacttagaag gtaaaaacat tgccctcttg tttgaaaaaa catcaactcg tacgcgtgca
    19621 gcttttacaa cagcagccat tgacctaggt gctcacccag aataccttgg tgccaatgac
    19681 atccaacttg gtaaaaaaga atcaactgaa gacactgcca aagtattggg tcgtatgttt
    19741 gatgggattg aattccgtgg ctttagccaa cgcatggttg aagaattggc agaattctca
    19801 ggtgtgccag tttggaatgg tttgactgat gaatggcacc caacccaaat gcttgctgac
    19861 tatttaacag tgaaagaaaa ctttggcaaa cttgaaggcc ttacccttgt ttactgtggt
    19921 gacgggcgta ataacgttgc caattccctt cttgtgactg gtgctatcct tggtgttaat
    19981 gttcatatct tctcaccaaa agaacttttc ccagaagaag agattgtgac attggctgaa
    20041 ggctatgcta aagaaagtgg cgctcgtatc cttatcacag aagatgctga cgaagctgtt
    20101 aaaggcgcag acgtgcttta cacagatgtt tgggtatcaa tgggtgaaga agacaaattc
    20161 aaagaacgtg ttgaattgct tcaaccatat caagtcaaca tggaccttgt tcaaaaagca
    20221 ggaaatgaca agcttatctt cttgcactgc ttgccagctt tccatgacac aaataccgtt
    20281 tatggtaaag acgttgctga aaaattcggc gtgaaagaaa tggaagtcac tgatgaagtc
    20341 ttccgcagca aatacgctcg tcattttgac caagctgaaa accgcatgca cactatcaaa
    20401 gcggttatgg cagcaaccct tggtaacctc tttattccaa aagtgtaata aggactggta
    20461 ctccttaact catttcacat atcaatgtga cccctttaat ttaacaattt aactacttaa
    20521 aaacgtactt taactacatg acaagtcgtt ctggttttcg cgtccttgac cggaactaga
    20581 acggcttatt tttatgaaaa ggtaagaggt aatcattatg acagaagaaa aaaaacgggg
    20641 ttttagaatt ccttcttctt acaccgttct ttttatcatt atagccatca tggcagtgtt
    20701 aacttggttt attccagctg gcgcctacga aaccgctaag ggcggtggtg ttatttcagg
    20761 aacctacaaa acagtagcat ccaatcctca aggatttttt gacatcttga tggctcccgt
    20821 tcgaggcatg ttaggtgttg aaggcactga tggtgctatt caagtgtctt tctttatctt
    20881 aatggttggg ggctttttag gagttgtcaa caaaacaggt gctcttgaca cagggattgc
    20941 gtcagtggtt cgtaaaaaca aaggtagaga aaaaatgctg attgctattt taatcccttt
    21001 gtttgctcta ggtggaacga cctatggtat gggggaagaa accatggcct tttacccact
    21061 tttaattcct gttatgattg cggttggttt tgacagtatt gttgctgtag ccattatttt
    21121 gattggttct caaattgggt gtctagcctc cactattaac ccatttgcaa ctggtgtagc
    21181 agctgatgct gctggtgtca gcattgcaga tggaatgatt tggcgtgtta tccaatgggt
    21241 tattcttgtc ggcatgtcta tttggtttgt ttacaattac gctagtaaga ttgaagaaga
    21301 cccaagtaaa tcattggttg cagacaagga agaagagcat aaggaactct tccagttgca
    21361 aaattctgga gaagacttaa acaagcgcca acgaaacgtt ttgaccattt ttactttgac
    21421 ctttgtcatt atgattctta gtttgattcc gtgggaagat tttggcatca aattctttac
    21481 taatatcaat acttggttaa ccacaatgcc tatcttaggg ggagttattg ggaaaaccat
    21541 gggagcattt ggtacttggt atttcccaga aatcaccatg ctctttatca tgatgggtgt
    21601 cttagttgct attgtgtatc gtatgagtga agaagacttt tttagctctt ttttgactgg
    21661 tgcaggtgaa tttttaggtg ttgccatgat ctgtgctatt gcacgcggta ttcaagttat
    21721 catgaatggt ggtatgatta cagccactat cttacactta ggtgaaacaa gtctttctgg
    21781 tttatcttct caagtttttg tgatattagc ttatattttc tacctcccaa tgtccttctt
    21841 gattccatca acatcaggac ttgctggggc tacaatggga attatggcac cgcttggaca
    21901 attctcaaat gtccctgctc accttgttat tacagccttc cagtcagctt ctggaatctt
    21961 aaacatgatt tctccaactt cagcaatcgt tatgggagca cttgcgcttg gtcgcgttga
    22021 ccttggtact tggtggaaat tcattggtaa atttattgta atggtgatgc ttgtaagcgt
    22081 gctattactt gtagttgcaa cattctttta acagatagga ggttcctatg gaatcctata
    22141 tcacacctaa acatcaagag gcctgtgtag ctgccattaa acagatcgtt tcttaccctt
    22201 ctgtttgtca tgaaggagaa aatggcacac cttttggaca ggccattcaa gatgttctcg
    22261 aagcaacctt agacttatgt caaggtcttg gttttcatac ttatattgat cctgaaggtt
    22321 actatggcta tgctgagtta ggtgaccaaa aagaggtctt ggctattctt tgccatttgg
    22381 acgttgttcc agaaggagat cgtcagttat ggaagacaga tccttttgac tgtgttgagg
    22441 cagacggctg tttatttggt cgaggcactc aagacgataa agggcctact atgatggcgc
    22501 tatttgccac caaagccttg atagatgcag gcgttacctt taataaacgc attcgtttta
    22561 ttttcggaac agatgaagaa actttgtggc ggtgtatgaa tcgctacaat gatgtagaag
    22621 aacaggctac ctttgggttt gctcctgatt caagtttccc tttaacctat gccgaaaaag
    22681 gcctacttca ggcaaaatta gtggggaaag gttgtgataa actgtcactt gaaatcggac
    22741 aagcctacaa tgttgttcct gcaagagctt cttataaagg caataagctt gaggctttac
    22801 aaaaagaact agataagctt ggctttgagt acgtcgtcaa agagggagaa ttaacggttt
    22861 atggtctcgc ccaacatgct aaagatgctc ccgatggcat caacgctatg attaggcttg
    22921 ccaaggcttt agtggtcctt gaacctaaca aaacccttga ttttcttgct aatgtcattg
    22981 atgaagatgg cagagcactt aatatttttg gcagaatcga agatgaacct tcaggcaaat
    23041 taagctttaa tgccgcaggg cttaccttaa ccaaagacaa ggcagaaatt cgcctagata
    23101 ttcggattcc agttttggca gataaggaaa aattagtcca acaattaagc caaaaagcac
    23161 aagaatacgg cctaacctat gaagaatttg attatttggc cccattatat gtgccattag
    23221 acagtgaatt ggtaacaacc ttgttatctg tctatcggaa aaaaacaggt gatcaaagcc
    23281 ctgcccaatc atcaggaggg gcaacctttg ctagaacaat gaagaattgt gttgcctttg
    23341 gtgccctttt ccctgatgct gttcaaacgg aacaccaaga aaatgaacat attgttttag
    23401 cggatgctta ccgcgctatg gatatttatg cggaagctat ttaccgacta acgcgataac
    23461 aagatatgag gagtaattat gacgaaacaa aaaatcgtag tcgcacttgg aggaaatgct
    23521 attctttcaa cagatgcgtc agcaaaagca caacaagaag ccttgatgtc cacatcaaaa
    23581 tcattggtta aattaattaa agaaggtcat gaagtcatcg tgacccacgg caatggccca
    23641 caagttggta atctcttatt acaacaagca gcagcagatt ctgaaaagaa tccggctatg
    23701 cctttggata cttgtgttgc catgacagaa ggtagcatcg gtttttggtt agttaatgcc
    23761 cttgataatg aattgcaagc ccaaggtatt caaaaagaag tggcagctgt tgtcactcaa
    23821 gtgatcgtgg atgccaaaga cccagctttt gaaaacccaa caaaaccaat tggtccattt
    23881 cttaccgaag aagatgccaa aaaacaaatg gcggagtctg gtgcaagttt caaagaagat
    23941 gcaggtcgtg gctggcgtaa ggttgtgcca tcaccaaaac cagttggtat taaagaagcc
    24001 aacgtcatca gaagtctggt tgattctggc gttgttgtgg ttagtgctgg tggtggcggt
    24061 gtgcccgttg tggaagatgc aactagcaag acccttacgg gtgtggaagc cgttattgac
    24121 aaggactttg ctagccaaac cctttcagaa ttggtggatg cagacctctt tatcgttttg
    24181 acaggggttg acaatgttta tgttaacttc aacaaacctg atcaaacaaa attggaagaa
    24241 gtaacggtct ctcaaatgaa agagtacatc acacaagacc aatttgcacc aggtagtatg
    24301 ttaccaaaag tggaagctgc cattgctttt gttgaaaaca aaccaaatgc taaagccatt
    24361 atcacatcgc tcgaaaatat tgacaatgta ctatcagcaa acgctggaac acaaattatc
    24421 gcagggtaat caagaggatt tttagttctg tttaaaagac tcatttaatg gtattttctg
    24481 aagagtacga ctgacataaa gagtttatac tgtttttaaa ggaaaagagt gtgccataat
    24541 aaaaaacaat aatagtatta gaaaaggcta attttattga gaattagcct tttcttttgg
    24601 ctttgatagg tgtataataa ccatactatt ttttattttt caaaatatta gaataaacaa
    24661 aagtagacaa cgtataaaga gaactgctaa gtcgttgagt tcattagtcc caaaaagatt
    24721 accaaaaaag aaggaagaac tatgaagaaa agttttattc atcaacaaga ggagatttcc
    24781 tttgttaaaa atacgttcac tcagtattta attgctaagt tagatgttgt tgaagtgcaa
    24841 ggacccattt taagtcgggt cggggatggt atgcaagata atttgtccgg tactgaaaat
    24901 ccagtatctg tcaatgtctt gaaaattcct aatgcaacct ttgaagttgt gcattcatta
    24961 gcaaaatgga aacgtcacac cttagcccgt tttgggttta atgaaggtga aggccttgtt
    25021 gttaatatga aggctctccg acctgatgag gattcacttg accaaaccca ttctgtctat
    25081 gttgaccaat gggactggga aaaagtcatc cctgatggca aacgtaatct cgcttacctc
    25141 aaagaaaccg ttgaaaccat ttacaaggtc attcggctaa ctgaattagc tgtagaagct
    25201 cgttatgata ttgaggctgt tttacctaag aaaataacct tcattcatac cgaagaattg
    25261 gtagcgaaat accctgattt gacgcccaaa gaacgtgaaa atgccattac taaagaattt
    25321 ggagcagtct ttttaattgg tattggcggg gttttacctg acggtaaacc acacgatggc
    25381 cgcgctcctg actatgatga ctggacaaca gaaactgaaa atggctatca tgggcttaat
    25441 ggtgatattt tggtttggaa tgaccaactt ggttcagcct ttgaattatc ctcaatgggg
    25501 atccgtgtgg atgaagaggc cctaaaacgt caggtggaaa tgactggtga ccaagaccgc
    25561 ttagcctttg attggcacaa atctttgttg aatggacttt tccctctaac gattggtgga
    25621 ggaatcggtc aatcacgtat ggtcatgttt ttgttgcgta aaaaacatat tggagaagtt
    25681 cagacctctg tttggcccca agaagttcgt gatagctacg ataatatttt ataagtaatc
    25741 acatcatttt gctaagtttt ccacttgtat aagagacttt aaataaatga tcagaaatat
    25801 tgaaaaaaga aatcattttt aaggtttaga gctagaaatt tgctataata aatgttatga
    25861 gagttgtatc aggtgaattt ggtgggcgtc ctttaaagac gctcgatgga aagataacac
    25921 gccctacttc agacaaagtt agaggtgcga tttttaatat gataggacct tactttaatg
    25981 gtggtcgtgt tttagatttg tttgcaggtt caggtggatt agccattgag gctgtttctc
    26041 gtgggatgtc tgctgcagtt ttggttgaga agaatcgtaa agcgcaagct attattcaag
    26101 acaatattat aatgactaaa gctgagaatc ggtttacctt gttaaaaatg gaagctgagc
    26161 gagctattga ttgcttgact ggtcgatttg acttagtctt tttagaccct ccttatgcca
    26221 aagaaacaat tgtggcaact attgaagctt tagcagctaa aaacttattg agtgaacagg
    26281 ttatggttgt ctgtgagaca gataaaacag tcttgttgcc aaaagagatt gcaacgttag
    26341 gtatttggaa agaaaaaatt tatggaatta gtaaggtaac agtttatgtt aactaaaata
    26401 ggactttata cagggtcatt tgatcctgtt acaaacggtc atctcgatat tgttaaacgg
    26461 gcaagtggcc tatttgatca aatatatgtt gggatttttg ataatcctac caaaaaaagc
    26521 tattttaaac tagaagttcg taaagcaatg cttactcaag ctctggctga ttttacaaat
    26581 gtaattgtag taacttcgca cgaacgttta gctattgatg tagctaaaga attacgagta
    26641 actcacttga ttagaggttt gcgaaatgca actgactttg agtatgaaga aaatttggaa
    26701 tattttaatc accttttagc tccaaatatt gagaccgtct atttgatatc aagaaataaa
    26761 tggcaggccc ttagctctag tcgtgtgaga gagctaattc attttcagtc ttccttagaa
    26821 ggtttggttc ctcagtcggt tattgctcaa gtggagaaaa tgaatgaaaa gacttaaaaa
    26881 aatcaaatgg tggttagtgg gtctgctagc tttaatctct ttgttgctag cgttattttt
    26941 tccgctacct tattatattg aaatgcctgg aggcgcttac gatattcgga ctgtcttaca
    27001 agtcaatggc aaagaagaca aacgaaaagg ggcttaccag tttgttgcag tgggcattag
    27061 tcgtgccagc ctcgctcagc tattatatgc ttggctgaca ccgtttactg aaattagtac
    27121 agcagaagat acaacaggcg gatacagcga tgctgatttc cttcgaatta atcaatttta
    27181 catggaaaca tcacaaaatg cagctattta tcaagcttta tccttagctg gaaaaccagt
    27241 tacattagat tataaaggcg tatatgtttt agacgtaaac aacgaatcta cttttaaagg
    27301 aacgttacac ttagcagata ctgtaacagg tgtaaatggt aaacagttta ctagttcagc
    27361 agaacttatt gactatgttt ctcacctaaa actaggggat gaagttacgg ttcagtttac
    27421 gagtgataat aagcctaaaa aaggagttgg ccgtattatc aaactgaaaa atgggaaaaa
    27481 tgggattggc attgccttga ctgatcatac aagtgtcaat tcagaagaca cagtgatctt
    27541 tagtactaaa ggagtaggag gacctagtgc tggtctaatg tttactcttg atatatatga
    27601 tcaaataact aaagaagatt tacgcaaggg ccgtacaatt gcaggtacag gaactattgg
    27661 caaggatggc gaagtaggag atattggtgg tgcaggtctt aaagtagttg cagcagctga
    27721 agctggtgca gatatatttt ttgttccgaa taatcctgtt gataaggaaa ttaaaaaag
//
LOCUS       DAUCLR010000017        27681 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.017, whole genome shotgun sequence.
ACCESSION   DAUCLR010000017 DAUCLR010000000
VERSION     DAUCLR010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27681
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.017"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..145
                     /locus_tag="VGT87_000569"
     CDS             <1..145
                     /locus_tag="VGT87_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEQ3813451.1"
                     /translation="KLKDKKQSHYKRLFWSNAIVSVLTNWISDDMIVPAEEMAAMGLP
                     LLT"
     gene            complement(182..1006)
                     /locus_tag="VGT87_000570"
     CDS             complement(182..1006)
                     /locus_tag="VGT87_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEQ3813452.1"
                     /translation="MIQLIAIDLDGTLLNQDKQIPKENITAIQEAAQSGLKIVLCTGR
                     PQSGTRPYFDQLGLTQEEFLIINNGCSTYSSPDWQLRHSKMLKVSDIELLEELSQSFP
                     DIYLTLTEENDYLVLEEEVPDLVQEDGDLVFTIVKPVSLAELSDTPRLIFQAMYLGEK
                     AALDAFERAVRNQLSQSFHVVRSQDNILEILPQGVSKASALKELVEDLGLTADQVMAI
                     GDAPNDIEMLTYAGLGVAMENASAAIKPLADKVTLTNDMAGVAQAIRQFALAAQKD"
     gene            complement(1008..1625)
                     /locus_tag="VGT87_000571"
     CDS             complement(1008..1625)
                     /locus_tag="VGT87_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEQ3813453.1"
                     /translation="MTIDMWEILNIIGTIAFALSGAIVAMEEEFDILGIFILGFVTAF
                     GGGAIRNTLIGLPIEALWGQKPEFTCAFFAMVLIMLFPKLIARGWVRAAVLTDAIGLA
                     AFSVQGALHAVRLNQPLSAVIVAAVLTGAGGGVVRDILAGRKPSVLRSEIYAGWSILA
                     AIVLHFKLADSTIECYALVVLLTTLRMIGNRKKWNLPKIKWKEER"
     gene            complement(1822..2799)
                     /gene="lacD"
                     /locus_tag="VGT87_000572"
     CDS             complement(1822..2799)
                     /gene="lacD"
                     /locus_tag="VGT87_000572"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEQ3813454.1"
                     /translation="MTITANKRHYLEKVSHQGIISALAFDQRGALKQMMAAHQEGEAT
                     VTQIETLKVLVSEELTPYASSILLDPEYGLLATKVRANQTGLLLAYEKTGYDATTTSR
                     LPDCLVEWSVKRLKAAGADAIKFLLYYDVDGDEQINLQKQAYIERIGSECTAEDIPFF
                     LELLSYDERISDNNSAAYAKLKPHKVNGAMSVFSDKRFGVDVLKVEVPVNMAYVEGFT
                     EGEVHYSQAEAIKAFQDQEAASHLPYIYLSAGVSAKLFQETLYFAAAAGAQFSGVLCG
                     RATWAGSVPVYITKGEDEARKWLCTEGFQNIDELNRVLEETASPWTEKI"
     gene            complement(2846..3299)
                     /locus_tag="VGT87_000573"
                     /pseudo
     CDS             complement(2846..3299)
                     /locus_tag="VGT87_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061423.1"
                     /note="catalyzes the formation of tagatose
                     1,6-bisphosphate from tagatose 6-phosphate and ATP;
                     frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
     gene            complement(3309..3824)
                     /gene="lacB"
                     /locus_tag="VGT87_000574"
     CDS             complement(3309..3824)
                     /gene="lacB"
                     /locus_tag="VGT87_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEQ3813455.1"
                     /translation="MKIAIGCDHIVTNEKMAVSDFLKSKGYDVIDCGTYDHTRTHYPI
                     FGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGIRSALVRDMTTALYAKEELNAN
                     VIGFGGKITGELLMCDIIDAFIKAEYKETEENKKLIAKIAHLETHHANQEDPDFFTEF
                     LEKWDRGEYHD"
     gene            complement(3839..4264)
                     /gene="lacA"
                     /locus_tag="VGT87_000575"
     CDS             complement(3839..4264)
                     /gene="lacA"
                     /locus_tag="VGT87_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEQ3813456.1"
                     /translation="MAIILGADAHGNALKELIKSFLQEEGYDIIDVTDINSDFIDNTL
                     AVAKAVNEAEGRLGIMVDAYGAGPFMVATKLKGMVAAEVSDERSAYMTRGHNNARMIT
                     IGAEIVGPELAKNIVKGFVTGPYDGGRHQIRVDMLNKMA"
     gene            complement(4521..5972)
                     /locus_tag="VGT87_000576"
     CDS             complement(4521..5972)
                     /locus_tag="VGT87_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEQ3813457.1"
                     /translation="MMQPFLDIINKILGFPIQLGSGVVMLIVMTGLAMIFGVKFTKAL
                     EGGIKLAIALTGIGAIIGILTGAFSESLQAFVKNTGISLNIIDVGWAPLATITWGSPY
                     TLYFLLVMLVVNIVMIVMKKTDTLDVDIFDIWHLSITGLLIMWYADHNHLPVLVSLLI
                     ATVAVILVGVLKIINSDLMKPTFDDLLGTGSQSPMTSTHMNYMMNPIIMVLDKIFDKV
                     FPGLDKYDFDAAKLNKKIGFWGSKFFIGMALGFVIGIMGDPHFSVESVKNWFGLGFTA
                     GACLELFSLIGSWFIAAVEPLSQGITNFANARMHGRRFNIGLDWPFIAGRAEIWACAN
                     ILAPIMLIESVLLSKVGNGILPLAGIIAMGMTPALLVVTRGRLIRMIIFGSLLLPLFL
                     LSGTMIAPFATELAKKVGAFPAGTSAGSLITHSTLEGPMEKIFGYVIGQATTGQIASI
                     ITLIIFVAIYLGLFAWYANQMKARNAEYAKTMK"
     gene            complement(5998..6303)
                     /locus_tag="VGT87_000577"
     CDS             complement(5998..6303)
                     /locus_tag="VGT87_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEQ3813458.1"
                     /translation="MIKILAACGAGVNSSHQIKDAIETQMSDRGYDVHCDAVMVKDIT
                     EELVSRYDIFTPIAKTDLGFEMPIPTVEAGPILYRIPIMSEPVFAELERVIKENHLQ"
     gene            complement(6296..6769)
                     /locus_tag="VGT87_000578"
     CDS             complement(6296..6769)
                     /locus_tag="VGT87_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEQ3813459.1"
                     /translation="MFPNILFTEARTQPELFDLVASHLEKVGYVTQEYHQALVEREAV
                     FPTGLKVDLKDGSDILYAAIPHTETKYCLVDQVVYVRNSQALTFKHMINPEEDCLVTD
                     FFFIINSQNEGQTTILSNLITFFITKGNLSCLASLKDDKQAISNYLIEKGVFQHD"
     gene            7006..7776
                     /locus_tag="VGT87_000579"
     CDS             7006..7776
                     /locus_tag="VGT87_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEQ3813460.1"
                     /translation="MLKRERLLKITEIVNEQGIVTVNDIIQTLNVSDMTVRRDLDELE
                     KAGKLIRIHGGAQSITMPNKKERSNIEKQTVQTKEKWELASYATQLVNDGETIFIGPG
                     TTLECFAEQLKNRQIRIVTNSLPVFNILQDSETIDLILIGGEYRSITGAFVGSLASQN
                     ISSLKFAKAFISCNGIYKNDIATYSETEGEIQKLAFNNSIEKYLLVDNQKFNAYDFFI
                     FYHLNNIDAVVTDSQITEDVIERYSQFTQLLVAHENKE"
     gene            complement(7980..8183)
                     /locus_tag="VGT87_000580"
     CDS             complement(7980..8183)
                     /locus_tag="VGT87_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEQ3813461.1"
                     /translation="MEKHYQVTGMTCDGCARTVTEKLSAVPGVQSVQVNLEKGEAKVT
                     GRPLTFLLKRALKDTKFELHKWS"
     gene            complement(8197..10428)
                     /locus_tag="VGT87_000581"
     CDS             complement(8197..10428)
                     /locus_tag="VGT87_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEQ3813462.1"
                     /translation="MAKEIFLVEGMSCASCALTIEKTVNQLPEVEQAVVNLATEKLTV
                     TYQDDDLETAKVIQAVKAAGYGAQVFDEHAKHMQTGRRDEDLKVIWSQVLWSALFTIP
                     ILYLAMGHMVGLWLPSFLQPDRYPFVYSFSQLLLTIPVLVLNRHYYHNGFKALFKGHP
                     NMDSLVALATSFAFAYSLYGVVEISLGRAHFVHSLYFESVVVILTLIGLGKYFESRSK
                     GRTYQAIQKLLSLKATDVRVWRNSQWQLLPLEEISYDDLVLVQPGEKVSIDGIIVEGH
                     SSLDESFLTGESLPVEKGEKDNVFAGSLNGQGALTIKPDKLGNDTLLAQIIQLVENAQ
                     ENKAPIAAIADRVSGVFVPVVLVLALLTGVFWLVFIQESLRFSLTTAIAVLVIACPCA
                     LGLATPTAIMVGTGRAAENGILFKGGDILEQAHQVDTVVFDKTGTITEGKPSLQNLLT
                     FSGDDHLILQEAASLEAYSQHPLGEAIVRAAQEAGYDSLPVEEFESLTGLGVTGQLEG
                     RRLAIGNATLMATLGIDLASVQSVCAKASGKGQTLVYYAKEGQLRALFSIADAVKEDS
                     QATVEALQQLGIHTIMLTGDHDATAKAIASQVGITDVISQVLPDQKAGVITNLQSQGR
                     KVAMVGDGINDAPALAVADIGIAMGSGTDIAIESADVILMKPDMLDLVKAMSLSRATM
                     RIVKENLFWAFIYNVLMIPVAMGLLHLFGGPLLNPMLAGFAMSFSSVSVVLNALRLKG
                     KTI"
     gene            complement(10428..10862)
                     /locus_tag="VGT87_000582"
     CDS             complement(10428..10862)
                     /locus_tag="VGT87_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEQ3813463.1"
                     /translation="MQQISAAEWEVMRVVWASGDIKSSDIITILRKKYQWSDSTIKTL
                     IGRLVKKNLLTSYREGRAYIYQALLDETLLQKEALATVLDGICQRQHTRLLLERLYDL
                     PMTLEEIEAFQELLEVKKENAVLEVPCNCLPGQCHCQKKETY"
     gene            11034..12020
                     /locus_tag="VGT87_000583"
     CDS             11034..12020
                     /locus_tag="VGT87_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEQ3813464.1"
                     /translation="MKHPIRKTLVTLGLLLTLCLPTPVAASSRSWKSWFIEQYFWLKR
                     DKSYYSKQDDPSFQRYLDACREQSDKPYQLDTNLVNGPLVQENLYGMQVYSWNDNGKP
                     DQKTIIYLAGGSYLNNPTTYHINMLKTLSTSLDAKIVLPIYPKAPRYTYNYTMPKLVN
                     LYQHYYHKNQNIFLMGDSAGGGLALGLAHALHNESVPQPKQLVLLSPWLDVTMSHPEI
                     PEYEDADPILSSWGLKRVGELWAYSADNTNHIYVSPKNGPITYLPPITLFTGTREIFY
                     PDIRDYAAKLKAANHNITFITQEGMNHVYPIYPIEEAKTAQYQIIDAINKTP"
     gene            complement(12154..12504)
                     /gene="rbfA"
                     /locus_tag="VGT87_000584"
     CDS             complement(12154..12504)
                     /gene="rbfA"
                     /locus_tag="VGT87_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEQ3813465.1"
                     /translation="MANHRIDRVGMEIKREVNDILQKKVRDPRVQGVTITEVQMQGDL
                     SLAKVYYTIMSDLASDNQKAQTGLEKATGTIKRELGKQLTMYKIPDLVFEKDNSIAYG
                     NKIDQLLRDLDNKS"
     gene            complement(12710..15571)
                     /gene="infB"
                     /locus_tag="VGT87_000585"
     CDS             complement(12710..15571)
                     /gene="infB"
                     /locus_tag="VGT87_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEQ3813466.1"
                     /translation="MSKKRLHEIAKEIGKSSKEVVEHAKYLGLDVKSHASSVEEADAK
                     KIISSFSKASKPDVTASQTVKPKEVAQPSVTVVKETGSEHVEKTQVSKPKSRNFKAER
                     EARAKEQAARKQANGSSHRSQERRGGYRQPNNHQTNEQGDKRITHRSQGDTNDKRIER
                     KASNVSPRHDNHQLVGDRNRSFAKENHKNGRFTNQKKQGRQEPQSKSPKIDFKARAAA
                     LKAEQNAEYSRQSETRFRAQQEAKRLAELARQEAKEAALKAQAEEMSHREAALKSIEE
                     AETKLKSSNINAKSTADNRRKKQARPEKNRELTHHSQEGQKKNKKSWNSQNQVRNQKN
                     SNWNKNKKTKKGKNVKNTNTAPKPVTERKFHELPKEFEYTEGMTVVEIAKRIKREPAE
                     IVKKLFMMGVMATQNQSLDGDTIELLMVDYGIEAKAKVEVDDADIERFFEDENYLNPE
                     NIVERAPVVTIMGHVDHGKTTLLDTLRNSRVATGEAGGITQHIGAYQIEEAGKKITFL
                     DTPGHAAFTSMRARGASVTDITILIVAADDGVMPQTIEAINHSKAAGVPIIVAINKID
                     KPGANPERVIAELAEYGIISTAWGGECEFVEISAKFNKNIDELLETVLLVAEVEELKA
                     DPTVRAIGTVIEARLDKGKGAIATLLVQQGTLHVQDPIVVGNTFGRVRAMVNDLGRRV
                     KSAEPSTPVSITGLNETPMAGDHFAVYADEKAARAAGEERSKRALLKQRQNTQRVSLD
                     NLFDTLKAGEIKTVNVIIKADVQGSVEALAASLVKIEVEGVRVNVVHSAVGAINESDV
                     TLAEASNAVIIGFNVRPTPQARQQADTDDVEIRLHSIIYKVIEEVEEAMKGKLDPVYQ
                     EKILGEAIIRETFKVSKVGTIGGFMVINGKVTRDSSVRVIRDSVVIFDGKLASLKHYK
                     DDVKEVGNAQEGGLMIENFNDLKVDDTIEAYIMEEIVRK"
     gene            complement(15591..15893)
                     /locus_tag="VGT87_000586"
     CDS             complement(15591..15893)
                     /locus_tag="VGT87_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEQ3813467.1"
                     /translation="MTNLERLSSLIGLAQRAGKVISGEELVVKAIQHQQVILVFLAND
                     AGPNVTKKVTDKSNYYNVEVSTVLNALELSAALGKPRKVAAIADAGFSKKMRTLME"
     gene            complement(15886..16182)
                     /locus_tag="VGT87_000587"
     CDS             complement(15886..16182)
                     /locus_tag="VGT87_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEQ3813468.1"
                     /translation="MSKVKKIPLRKSLVSGEIIAKRDLLRIVKTKDGQVFIDPTGKQN
                     GRGAYIKLDNQEALMAKKKQVFNRSFSMDIPESFYDDLIAYVDHKIKRRELGLD"
     gene            complement(16198..17355)
                     /gene="nusA"
                     /locus_tag="VGT87_000588"
     CDS             complement(16198..17355)
                     /gene="nusA"
                     /locus_tag="VGT87_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEQ3813469.1"
                     /translation="MSKEMLEAFRILEEEKHIDKADIIDAVTESLKSAYKRRYGQSES
                     CVIEFNEKTADFQVFTVREVVEEVFDSRLEISLKDALAISSAYELGDKIRFEESVNEF
                     GRVAAQSAKQTIMEKMRRQMREVMFNEYKEHEGEIMTGTVERFDQRFIYVNLGSLEAQ
                     LSHQDQIPGETFKSHDRIDVYVYKVENNPKGVNVFVSRSHPEFIKRIMEQEIPEVFDG
                     TVEIMSVSREAGDRTKVAVRSHNPNVDAIGTIVGRGGSNIKKVISKFHPKRVDAKTGL
                     EIPVEENIDVIQWVDDPAEFIYNAIAPAEVDMVLFDDEDLKRATVVVPDSKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEYDRLEAEKEAATAVEELVVDDVMTAEKV"
     gene            complement(17530..18066)
                     /gene="rimP"
                     /locus_tag="VGT87_000589"
     CDS             complement(17530..18066)
                     /gene="rimP"
                     /locus_tag="VGT87_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEQ3813470.1"
                     /translation="MDSQGPIILEKSIKIEEVIKIANTSIIDIVTKTVTPEIKAPYEL
                     VDVEYDKMGSDYILSILVDKEGGITVEDTSDLTNIISPLLDTIDPDPFPNQYMLEVSS
                     PGLERPLKTADSLKAAVGSYINVSLYQAIDKVKVFQGDLLAFDGETLTIDYLDKTRHK
                     IVNIPYQAVAKVRMAVKL"
     gene            complement(18174..18260)
                     /locus_tag="VGT87_000590"
     tRNA            complement(18174..18260)
                     /locus_tag="VGT87_000590"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18224..18226),aa:Ser,seq:gga)
     gene            complement(18325..18960)
                     /gene="trmB"
                     /locus_tag="VGT87_000591"
     CDS             complement(18325..18960)
                     /gene="trmB"
                     /locus_tag="VGT87_000591"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEQ3813471.1"
                     /translation="MRVRKRKGAEEHLANNPHYVILNPEDAKGRWHDVFGNDRPIHIE
                     VGSGKGGFITGMALKNPDINYIGIDIQLSVLSYALDKVLASEVPNVKLLRVDGSSLTN
                     YFEDGEVDMMYLNFSDPWPKTKHEKRRLTYKDFLDTYKRILPEHGEIHFKTDNRGLFE
                     YSLASFSQYGMTLRQIWLDLHASNYEGNVMTEYEEKFSNKGQVIYRVEANF"
     gene            complement(18960..19751)
                     /locus_tag="VGT87_000592"
     CDS             complement(18960..19751)
                     /locus_tag="VGT87_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEQ3813472.1"
                     /translation="MTTTEQELTLTPLRGKSGKAYKGTYPNGECVFIKLNTTPILPAL
                     AKEQIAPQLLWAKRMGNGDMMSAQEWLNGRTLTKEDMNSKQIIHILLRLHKSKKLVNQ
                     LLQLNYKIENPYDLLVDFEQNAPLQIQQNSYLQAIVKELKRSLPEFKSEVATIVHGDI
                     KHSNWVITTSGMIFLVDWDSVRLTDRMYDVAYLLSHYIPRSRWSEWLSYYGYKNNDKV
                     MQKIIWYGQFSHLTQILKCFDKRDMEHVNQEIYALRKFREIFRKK"
     gene            complement(19815..20849)
                     /locus_tag="VGT87_000593"
     CDS             complement(19815..20849)
                     /locus_tag="VGT87_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ3813473.1"
                     /translation="MKALFLKRRQDFQKQQNKYLRYVLNDHFVLVLMFLLGFAMVQYG
                     QLLNHFPTNHLPIQVCLGILIPLLLSMGSIATYLEEADQHFLLPKEEEVISYIKQAER
                     LSFLLWGTLQTAVLLFLYPIFRRLGLSLFIFIILVLILLALKRVVLSRKTRYFLRGNR
                     LDWAKAVAFESNRKQSILKFYSLFTTVKGISTKVKERTYLNPLLKLVKQTPSNLWLSL
                     YARAFLRSSDYLGLFLRLMLLSSLSVFFIHNLYLSVSLALIFNYLVVFQLLSLYYHYD
                     YHYMTSLYPENSRSKKKNVLSFLRGLSFLMLIVNMLCCSSAPKALILIVGMVFIACIY
                     LPYKLKKIID"
     gene            complement(20852..21577)
                     /locus_tag="VGT87_000594"
     CDS             complement(20852..21577)
                     /locus_tag="VGT87_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ3813474.1"
                     /translation="MLNIKNLTGGYHNIPVLNDVSFSVDNGELVGLIGLNGAGKSTTI
                     NEIIGFLKPYQGSISIDGLTLAENAVAYRQKIGFIPETPSLYEELTLSEHINTVAMAY
                     DIDLEVAQKRAQPFLEMFRLTDKLEWFPVNFSKGMKQKVMIICAFVIDPSLFILDEPF
                     LGLDPLAISDLIQTLEVEKAKGKSILMSTHVLDSAERMCDRFVILHHGQVRAQGTLAD
                     LQEAFGDRSASLNDIYLALTKED"
     gene            21649..22068
                     /locus_tag="VGT87_000595"
     CDS             21649..22068
                     /locus_tag="VGT87_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEQ3813475.1"
                     /translation="MENCIFCSIIQGDIPSSKVYEDEQVLAFLDISQTTKGHTLVIPK
                     QHVRNLLEMTAETASHLFARIPKIARAIQSATGATAMNIINNNEALAGQTVFHAHVHL
                     VPRYNEEDGISIQYTTHEPDFPVLEKLARQINQEVSS"
     gene            22065..22421
                     /locus_tag="VGT87_000596"
     CDS             22065..22421
                     /locus_tag="VGT87_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HEQ3813476.1"
                     /translation="MKVKTMIAVGLLSFTAYKAYQKRCTIKELLSISRQAKDAAQLDL
                     DNIKANLDLIHSQGKVIQNISQDLAHKWRYFNQETQAHLTEIQNRMAKYQGDSELISS
                     ESPTCQTVILTNNHSR"
     gene            complement(22532..23815)
                     /locus_tag="VGT87_000597"
     CDS             complement(22532..23815)
                     /locus_tag="VGT87_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEQ3813477.1"
                     /translation="MKIGKKIVLMFTAIVLTTVLALGVYLTSAYTFSTGELSKTFKDF
                     STSSKKSDAIKQTRAFSILLMGVDTGSSERASKWEGNSDSMILVTVNPKTKKTTMTSL
                     ERDTLTTLSGPKNNEMNGVEAKLNAAYAAGGAQMAIMTVQDLLNITIDNYVQINMQGL
                     IDLVNAVGGITVTNEFDFPISIAENEPEYQATVAPGTHKINGEQALVYARMRYDDPEG
                     DYGRQKRQREVIQKVLKKILALDSISSYRKILSAVSSNMQTNIEISSRTIPSLLGYRD
                     ALRTIKTYQLKGEDATLSDGGSYQIVTSNHLLEIQNRIRTELGLHKVSQLKTNATVYE
                     NLYGSTKSQTVNNNYDSSGQAPSYSDSHSSYANYSSGVDTGSSYQSASTDQDSTASSH
                     TPTTPSSSSDALAADESSSSGSGSLVPPANINPQT"
     gene            complement(23824..24348)
                     /locus_tag="VGT87_000598"
     CDS             complement(23824..24348)
                     /locus_tag="VGT87_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ3813478.1"
                     /translation="MIQQSLVIEEGVPQDAKAVLSLVTAAAKETDFITGVETILEATP
                     QELSDFLSRSRTSFIDFCLLARLDEKVVGLLNLSGEVLSQGQAEADVFMLVAKTYRGY
                     GIGQLLLEIALDWAEENPYIESLKLDVQVRNTKAIYLYKKYGFRIESMRKNDIKSKNG
                     DDLDVYHMRKSVSQ"
     gene            complement(24323..24784)
                     /gene="tsaE"
                     /locus_tag="VGT87_000599"
     CDS             complement(24323..24784)
                     /gene="tsaE"
                     /locus_tag="VGT87_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEQ3813479.1"
                     /translation="MFYSENEYTLKAYGETLGTYLSIGDVIVLSGDLGAGKTTLAKGI
                     AKGMGISQMIKSPTYTIVREYEGRLPLYHLDIYRVGDDPDSIDLDDFLFGNGVTVIEW
                     GELLGEGLLQDYLQITITKRDKGRQLDLLAHGERSRQLLEIMSHDSAKSRY"
     gene            complement(24938..26398)
                     /locus_tag="VGT87_000600"
     CDS             complement(24938..26398)
                     /locus_tag="VGT87_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEQ3813480.1"
                     /translation="MEKFFKLSENGTTVSTEIMAGLTTFFAMSYILFVNPSILGAAGM
                     PSNAVFLATIIAAAISTLIMGLFANVPYALAPGMGLNAFFTYTVVFALGFSWQEALAM
                     VFICGLFNIFITVTKFRKSIIKAIPVSLQHAIGGGIGVFVAYLGFKNANIITFSISAE
                     NIVMVNGVEPAKASAKTFADGLLFVDANGGVVPTISSFTDSSVLLAIFGLLLTTALVI
                     RNFRGAILIGIVATTLVGIPLGIVDVSNLNFGISHIGEAWTELGTTFLAAFDGLSSLF
                     SDSSRLPLVFMTIFAFSLSDTFDTIGTFIGTGRRTGIFSQDDENALENSIGFNSKMDR
                     ALFADAIGTSIGALVGTSNTTTYVESAAGIAEGGRTGLTAVSTAVCFLLSILLLPLVG
                     IVPAAATAPALIVVGVMMVSSFLDVNWSKFADALPAFFAAFFMALCYSISYGIAAAFI
                     FYCLVKVVEGKTKDIHPIIWGATFLFIVNFIILTIL"
     gene            26662..27474
                     /locus_tag="VGT87_000601"
     CDS             26662..27474
                     /locus_tag="VGT87_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEQ3813481.1"
                     /translation="MTKKLIAIDLDGTLLHHDNTISTYTQKTIKAVQDKGHHVIISTG
                     RPYRMALGYYLQLNLKTPIITFNGALTHMPEQKWAYEHNVTLDKGYLLRLLKYQDDFQ
                     MDFIASEYRKNVYITMTNPESIDPQLFGVDEITQDMALEITKITRNPNALLMQTRHED
                     KYALAKNMRAFFKDEIEIDSWGGPLNILEISSKNINKAYALNYLLGIYNMDKKDLIAF
                     GDEHNDTEMLAFAGTGYAMKNASPVLLPYADQQLNFSNEEDGVAKKLEELFL"
BASE COUNT         8516 a         5996 c         4736 g         8433 t
ORIGIN      
        1 aaaattaaaa gacaaaaaac aatcgcacta taagcgcctt ttttggagca atgctattgt
       61 ttctgtcttg acaaactgga tttcagatga catgatcgtt cctgccgaag aaatggctgc
      121 tatgggcttg cctctattaa cgtaaaaagg ttccttttgg gaaccttttt ttatataata
      181 gttagtcttt ttgagctgca agtgcaaact gacggatagc ctgggccaca cccgccatat
      241 cattagtcaa agtgactttg tcagctaatg gcttgatagc tgctgaagca ttttccatag
      301 caactcctaa accagcataa gttaacatct caatgtcatt gggagcatcc ccaatagcca
      361 tcacttggtc agcagttaaa cccaaatctt ctactaattc tttgagagcc gatgccttgc
      421 tgactccctg agggagaatt tctaaaatat tatcttggct acgaacaaca tgaaaactct
      481 gactaagctg atttctcacc gcacgttcaa aggcatctag cgcagctttt tctcctaagt
      541 acatggcttg aaaaatcagg cgaggagtgt ccgacagctc tgctaaggag acaggcttga
      601 caatggtaaa taccaaatct ccatcttcct gcacaaggtc tggcacttct tcttcaagca
      661 ccaaataatc gttttcctca gttaaagtca ggtaaatatc tggaaaactt tggcttagct
      721 cttccagcaa ttcaatatct gaaaccttca acatttttga atggcgtaat tgccagtcag
      781 gactactata agtactacag ccattattga taatgaggaa ttcctcttgt gtcaaaccta
      841 attggtcaaa atagggtctt gtccctgact gaggccggcc tgtgcataag acaattttta
      901 agcctgactg agctgcttct tggatggctg tgatattctc ttttggaatc tgtttgtcct
      961 ggtttaataa ggtgccgtcc aaatcaattg caattagttg aatcatccta tctttcctct
     1021 ttccatttga ttttagggag gttccatttt ttacggttgc caatcatgcg taaagtcgtg
     1081 agtaaaacaa ccaaggcata acattcaatc gttgaatctg ctagtttaaa atgcaaaaca
     1141 atagctgcaa gaatagacca cccagcatag atttcactac gcaaaacact aggttttcgc
     1201 cctgccagaa tatctctgac aacacctccg cctgcaccag tcaacacagc tgctacaatg
     1261 acagcactta aaggctggtt aagacggaca gcatgcaagg ctccttggac actaaaagct
     1321 gccagaccaa tggcatcagt caaaacagct gcccgtaccc aacctctagc tattagctta
     1381 ggaaaaagca taatcaaaac catagcaaag aaagcgcaag tgaattctgg cttttgcccc
     1441 cataaggcct ctataggtaa accaattagg gtgtttcgaa tggctccgcc tccaaaggct
     1501 gtcacgaaac ctaaaataaa aatccctaaa atatcaaatt cttcttccat ggcaacaata
     1561 gcaccagaaa gagcaaaggc aatggtcccg atgatattta aaatttccca catatcaatt
     1621 gtcataagct accctattgt cttttagtct tcttttagtt taccacgttt tcctttaaaa
     1681 aataagtaga aaaagataaa atgtaaggat aaaaaagcaa ggataaaacc cattttcatc
     1741 cttgctacaa aagattcaaa caattatccc catacttatc ttattacatc aatgagagct
     1801 gcttgatagg ataaggttgt tttagatttt ttccgtccaa gggctagcgg tctcttcgag
     1861 taccctgttt aactcgtcaa tattttggaa gccttcagtg catagccact ttcttgcttc
     1921 gtcctctcct ttagtaatat aaactggaac ggatcctgcc cacgtggcac gcccacacaa
     1981 aacaccgcta aattgagcac ctgctgctgc tgcaaagtat aatgtctctt gaaaaagttt
     2041 ggcagatacg cctgcactca aatagatata agggaggtgg ctggcagctt cttggtcttg
     2101 aaaagcttta atagcctcag cttgactgta atggacttcc ccttcagtaa aaccttctac
     2161 ataagccata ttaacaggaa cctctacttt tagcacatca acgccaaaac gcttatccga
     2221 aaatactgac atagcaccgt taaccttatg tggcttaagc tttgcatatg ccgcactgtt
     2281 gttatctgat attctctcat cataactcaa aagttcaaga aaaaaaggaa tgtcttcagc
     2341 tgtacattct gaaccaatgc gttcaatata agcttgtttt tgtaaattga tttgttcatc
     2401 tccatcaaca tcataataga gcaaaaattt aatagcatct gcacctgctg ctttcaaccg
     2461 tttgaccgac cattccacta agcaatcggg taaacgacta gtggttgtag catcataacc
     2521 tgttttttcg taagctaaca gtaagcctgt ctgattagct cttaccttag tagccagcaa
     2581 cccatattct ggatctaata aaatagatga agcataaggc gtcaattcct cagaaacgag
     2641 aactttcaat gtttctatct gtgtaactgt cgcttctcct tcttgatgtg ctgccatcat
     2701 ttgcttcagt gcacctcgtt gatcaaaagc taaagctgat atgattcctt gatggctcac
     2761 tttttctaag taatgtcttt tatttgctgt gattgtcatg tgatacctct cttacttgaa
     2821 gttgctgata taattgttta aaagttgcca tagtcatcaa atcttgataa tagtgagtag
     2881 ctagtctctt aggcagactg cctgagaagt taagacatca aacggcttag caatagtact
     2941 catgtggctc aaagagactt cggcttcgtc cacatcactg tagggaccag cttccaaaat
     3001 ttttgtctgc atgccttcat gtaaaacagc aatacaattt ctcgtttccc ctttgatttt
     3061 gaaaaattgg ttagaaatgc cctgttcttg taactgatga atgataaagt cacccagttt
     3121 actaccacta aatcctgttg caataactga ccagcttttg ttaacattct ggtcacattt
     3181 agctccttat caccagctgt ttttgccgtt ctgtcaacac gattaaccgt gtccatagcc
     3241 agacaatcta aaggatagga aatagcgaca gaaggattta aggtaactgt taaactcatc
     3301 tcttcttcct aatcgtgata ctcaccacgg tcccattttt ctaaaaattc tgtaaagaaa
     3361 tcaggatctt cttggttagc atgatgagtt tccaagtggg ctattttagc aattagcttc
     3421 ttattctctt cagtttcttt gtattctgcc ttgataaaag cgtctataat atcacacata
     3481 agaagttcac cagtgatttt accaccaaag ccaatcacat tggcattcaa ttcttccttg
     3541 gcataaagcg ctgttgtcat atctcgaaca agggcggagc gaatacctgg taccttgttc
     3601 acggcattat taatgccaac tccagttcca caaatacaaa cacccaagtc tgcttgacca
     3661 ctaaccacag cttcgccgac tttttttcca aaaatagggt aatgggttct tgtatggtca
     3721 taagtgccac agtcaataac gtcatagcct ttagatttca aaaaatcaga aacagccatt
     3781 ttttcgttgg tgacaatatg gtcacaacca atcgcaattt tcatcacttt atcctccttt
     3841 aggccatttt attgagcatg tcaacacgaa tttgatgacg accaccatcg tagggaccag
     3901 tcacaaaacc tttgacgata ttcttcgcta attctggacc tacaatctct gcccctattg
     3961 tgatcatgcg ggcattgttg tgaccacgcg tcatgtaagc agagcgttca tctgacactt
     4021 cagcagcaac cattcctttt aattttgtgg ctaccataaa gggacctgct ccgtaagcat
     4081 ccaccataat gcctaaacgc ccttctgctt cattaacggc tttagccacc gctaatgtgt
     4141 tatcaataaa atcactgtta atatcagtca cgtcaataat gtcatagcct tcttcttgaa
     4201 gaaaactttt gatcaattct tttaatgcat ttccgtgtgc atctgcacct aaaataatag
     4261 ccatcgtttc tccttttcta atccaaatcg cttgccaatc tacaaaaaga taacaggatt
     4321 acctatcatt aggtcaggat tattgccagt aatcttagcg tcttccatta tttctttcaa
     4381 gacaaagcac ttgtgggttc ccacagagcg ccttgcctta catgagaaat actcttttca
     4441 cgttaggggg taaggcagaa ataccttacg cagttatctg ctattctccc taatttttta
     4501 gtcagtcacc tgacctgatt ttatttcatt gttttcgcat actcagcgtt acgtgccttc
     4561 atttggttag cataccaagc aaagagacct aggtaaatgg ccacaaagat gattaacgtg
     4621 atgattgagg caatctgacc tgttgtcgct tgaccaatca catagccaaa gattttttcc
     4681 attggtcctt caagtgttga atgcgtaatc aaggaaccag cgcttgtacc tgctggaaaa
     4741 gcccctactt ttttagccaa ctctgtcgca aatggagcaa tcatagttcc tgacaagagg
     4801 aataatggca ataagaggga accaaaaata atcatgcgaa tcaaacgacc acgtgtgacc
     4861 acaagaagag ctggtgtcat ccccatagca ataattcccg ccagtggtaa aatcccattg
     4921 ccaacttttg ataggagaac ggattcaatc aacatgattg gggcaagaat attggcacag
     4981 gcccagattt cagcgcgacc agcaatgaag ggccagtcca aaccaatatt gaaacgacgt
     5041 ccatgcattc tagcattggc aaaattggta atcccttgtg acaaaggctc aacagctgcg
     5101 ataaaccaag aaccaatcaa agagaaaagt tccaaacagg cacccgcggt aaagcctaga
     5161 ccaaaccaat ttttaacaga ttcaacacta aaatgaggat ctcccataat cccaatcaca
     5221 aaacctagag ccattccgat aaagaactta gagccccaga agccaatttt tttgttcaat
     5281 ttagcagcat caaagtcata tttatctagc cccggaaaga ccttgtcaaa aatcttatct
     5341 aagaccataa tgattggatt catcatgtag ttcatgtgag ttgaagtcat tggtgattga
     5401 gaccctgtcc caagaaggtc atcaaaggtc ggtttcatca aatcagagtt aatgattttc
     5461 aataccccaa caagaataac tgccacagta gcaatcaaaa gagacacaag gactggcaaa
     5521 tggttgtgat cagcatacca cataatcaat agacctgtga tagacaaatg ccaaatatca
     5581 aaaatatcga catcaagggt gtccgttttt ttcatgacaa tcatgacaat attaaccacc
     5641 agcatcacca gcaagaaata aagggtataa ggggaacccc aggtaattgt tgcaagcggt
     5701 gcccaaccga catcaataat attcaaactg attcctgtat ttttgacaaa ggcctgcaaa
     5761 gactctgaaa aagcccccgt caaaatccca ataatggctc caatacctgt aagagcaata
     5821 gctaacttaa tcccgccttc cagtgccttt gtgaatttaa ccccgaaaat catggctaaa
     5881 cctgtcatga caatcagcat gaccacacct gatcccaact gaataggaaa tcccagaatc
     5941 ttgttaataa tgtctaaaaa aggttgcatc atcttgtctc cttatgtttt tattaactca
     6001 ctgcaaatgg ttttctttaa tcacgcgctc taattcagca aaaacaggct cactcatgat
     6061 tgggatacga tagagaatcg gtcctgcttc tactgtggga attggcatct caaatcccaa
     6121 gtcagttttt gcaatcggcg taaaaatatc ataacgagac accaattctt ctgtgatgtc
     6181 tttgaccatc accgcatcac aatgcacatc atagccacga tcactcatct gagtttcaat
     6241 cgcatcttta atttgatggc ttgagttaac ccctgcaccg caggctgcta gaattttaat
     6301 catgctgaaa cactcctttt tctattaaat agtttgaaat agcttgttta tcatctttta
     6361 gactggctag acagctaaga ttgcctttcg taataaagaa ggtaataaga ttagatagaa
     6421 tcgtcgtttg accctcgttt tgactattga taataaagaa gaaatccgtt accaaacagt
     6481 cttcctctgg attaatcatg tgcttaaagg taagggcttg actatttcta acatagacca
     6541 cttgatcaac caagcaatac ttggtttctg tatgcggaat agccgcatac aaaatatctg
     6601 acccatcttt gaggtccact ttgagtcctg tcggaaatac cgcctcacgt tccactaaag
     6661 cctgatggta ctcttgcgtc acatacccaa ccttttccaa gtggctagca acaagatcaa
     6721 aaagctctgg ctgcgtcctt gcttcagtaa atagaatatt aggaaacact ccctaccgcc
     6781 tttctataag tatcaatttt taacaactgt catctcatca tcaacaaatt ggtaaccgtt
     6841 tacaaaatca ttatagtata accttcaaca taattcaaca tttttacgca atattttttg
     6901 ttctatatgt ttgtttttgt tttgtttatt ttgttttatt gaaatatgaa ggtctatatg
     6961 ctatgataaa cataagtaaa taaactatag aaaggagccc cttagatgtt aaaaagagaa
     7021 agacttctaa aaattaccga aatagtcaat gagcaaggta ttgttactgt gaatgatatt
     7081 attcagactt taaacgtttc tgatatgaca gttcgtagag acttagatga actggaaaaa
     7141 gccggcaaat tgatccgcat ccatggaggt gcacaaagta ttaccatgcc taataaaaaa
     7201 gagcggtcaa atattgaaaa acaaaccgtt caaacaaagg aaaaatggga actagcttca
     7261 tatgcgacac aactagtcaa tgatggtgaa acaatcttta ttggtccagg aacaacttta
     7321 gaatgtttcg ctgagcaatt aaaaaatcgt cagattcgca ttgtgaccaa tagcctccct
     7381 gtctttaaca tccttcagga cagcgaaacc attgacctga ttcttatcgg tggtgaatac
     7441 cgatctatta caggagcttt tgtagggtcc cttgctagtc aaaatatctc atccttaaaa
     7501 tttgccaagg cttttattag ctgtaatggc atttataaaa acgatattgc aacttatagc
     7561 gaaactgaag gagagattca aaagctagct tttaataatt ctattgaaaa atacctcttg
     7621 gtagataacc aaaaattcaa tgcctacgac ttcttcattt tttaccattt aaacaacatt
     7681 gatgctgttg ttacagattc gcaaataact gaggacgtca tcgaacgtta tagtcagttc
     7741 acacaattat tagtagccca cgaaaacaag gagtaaccgt ttcatcaaga atggacagcc
     7801 tgtcagcgca tcgtccttaa acaccttctg gcaaaagtga tttgccccca gagaggtgtt
     7861 tttgttttgc tcggaacaca cctgctaacg tcagagtctt tatgagtgac cacaaaaaaa
     7921 gaagcccaga ttaaaaaact ggaacctctt ttgagatgaa gtaaggctac caaaataacc
     7981 taagaccatt tatgtaattc aaatttggta tccttcaaag cacgcttgag taggaaagtt
     8041 aaaggacgtc ctgtcacctt agcctctcct ttttccaaat ttacctggac cgattgcaca
     8101 ccggggacag ctgataattt ttcagtaacc gttcttgcac aaccgtcaca cgtcattcct
     8161 gtaacctgat agtgtttttc catttcgttt tctccttcaa atggtttttc cttttaatcg
     8221 cagggcattc aaaacaactg atactgaact aaaactcatg gcaaaacctg ctaacatcgg
     8281 gttcaatagt ggacctccaa aaagatgaag taagcccatg gccactggaa tcatgaggac
     8341 attgtatatg aatgcccaaa ataaattttc cttgacaatc ctcatagtcg ctcgactcaa
     8401 tgacatagct ttcaccaaat ccagcatatc aggtttcatc agaatcacat ccgccgattc
     8461 aatggcaata tctgtccctg accccatagc tatcccaatg tctgctacag ccaatgccgg
     8521 agcatcatta ataccatcac ctaccattgc aacttttctc ccttgagatt ggaggtttgt
     8581 tatcacgcct gctttttgat caggtaagac ttgactgatc acatctgtaa tccccacttg
     8641 agaggcaata gccttagcgg tagcatcgtg gtcacctgtt agcatgatgg tgtgaattcc
     8701 cagttgctgc aaagcttcga cggtagcttg actatcttct ttgacagcgt cagcaatact
     8761 gaaaagagct cttagctgcc cctctttagc ataatataca agggtttgac ctttccctga
     8821 agccttagca caaactgact gaacagaagc taaatcaata ccaagggttg ccattaaagt
     8881 agcatttcca atcgccaaac gacgcccttc aagctgacca gtgactccta ggccggttaa
     8941 agactcaaac tcttccactg gtagactatc gtaaccagct tcctgagcag cacggacaat
     9001 agcctcccct aaggggtgtt gagaataggc ctccaaggaa gcagcttctt gcaagataag
     9061 gtgatcatct cccgaaaaag ttaggaggtt ttgaagactt ggtttcccct ccgtaatcgt
     9121 ccccgtttta tcaaaaacaa ccgtatctac ctgatgggct tgttctaaga tgtcaccacc
     9181 tttaaaaaga ataccatttt cagctgcacg ccctgttcca accataatcg ctgtcggggt
     9241 tgctaaacca agcgcacaag gacaagcaat gactagaaca gcgatggctg ttgtcaaact
     9301 aaaacgaaga ctttcctgta tgaataccaa ccaaaacacc cctgttaaca gagctaatac
     9361 caaaaccacc ggaacaaaga cacctgagac tctatcagca atggctgcaa taggtgcttt
     9421 attttcttga gcattctcaa ctagttggat aatttgtgct aacaaggtat cattcccaag
     9481 cttatctggt ttaatcgtga gggcgccttg accattcaag ctgcccgcaa agacattatc
     9541 tttttctccc ttttcaacag gcaaactttc cccagttaaa aaggattcat ccaaactgct
     9601 atgtccttcc acgataatgc cgtcaataga aactttttca cctggttgaa ccaggacaag
     9661 gtcatcatag ctaatctctt ctaacggcaa caattgccat tggctgtttc tccataccct
     9721 cacatcagta gccttcaagg ataggagctt ttgaatagct tgataagtac ggcccttaga
     9781 acgactttca aaatactttc ctagaccaat gagtgtcaaa atcactacca ccgattcaaa
     9841 ataaagggag tgcacaaagt gcgcccggcc cagactgatt tcaaccactc cataaagact
     9901 ataagcaaaa gcaaagctgg ttgctaaagc taccaaggag tccatattag gatgtccctt
     9961 gaacaaagct ttgaacccat tatggtaata atgacgattt aatactaaga ctggaatcgt
    10021 caagagtagc tgactaaagc tgtaaacgaa aggatagcga tctggttgca gaaatgaagg
    10081 aagccataag cccaccatgt gacccatagc taggtataat ataggaatgg tgaatagggc
    10141 agaccacaag acctgagacc aaataacctt taggtcttcg tcacgacgac ctgtttgcat
    10201 gtgttttgca tgttcatcaa agacttgtgc accataacca gcagctttta ccgcttgaat
    10261 aactttggcc gtttctaaat cgtcatcttg ataagtaacc gtgagtttct cggtcgctaa
    10321 attcacaaca gcctgttcta cttcaggcaa ttgattgacc gttttttcaa ttgttagagc
    10381 acaagaggca caactcattc cctccactaa aaatatctct ttagccatta atacgtctcc
    10441 tttttctgac agtggcactg cccaggcagg caattacacg gcacctcaag aactgcattt
    10501 tcttttttga cttctagtag ctcttggaat gcctcaattt cctctaaggt catcggtaag
    10561 tcataaagcc gttcaagaag tagacgcgtg tgttgccgct gacaaatacc gtctaaaaca
    10621 gtcgctagtg cttctttttg tagcaaagtc tcatccaaaa gagcttgata aatataggcc
    10681 ctaccttccc gataagatgt taaaagattt tttttaacta gcctaccaat gagggtctta
    10741 atagtggaat ctgaccattg atattttttc cttaaaatag taataatatc gctactttta
    10801 atatcaccac tggcccacac cacacgcatg acttcccatt cggcagctga tatctgttgc
    10861 ataacgtcac cttcattcac aatatattta cgtttgtaaa ttaattataa cgaatttaac
    10921 tacatttgta aataaataac ttttctcatt tttctatcct tatattgaca aatgaatcgt
    10981 ttttactata atattattgc aataaaaata acatttacat taaggagata cccttgaaac
    11041 atcctattcg aaaaacactt gtcaccttag gtctattact gaccttatgc ctgccgactc
    11101 cagttgcagc tagttctcgt tcttggaaga gctggtttat cgagcaatat ttctggttaa
    11161 aacgtgacaa gagttactat tctaaacaag atgacccaag cttccaaaga taccttgatg
    11221 cctgtcgtga acaatctgat aaaccttatc aactagatac taatttagtc aatggtcccc
    11281 ttgttcaaga aaatctttat ggcatgcagg tttactcttg gaatgataat ggaaaacctg
    11341 atcaaaaaac aattatttat cttgccggtg gttcttatct taacaatcca acgacatacc
    11401 atattaatat gttaaagaca ttatccacaa gtcttgacgc taaaattgtt ttacccattt
    11461 atcctaaagc cccacgttat acctataact atactatgcc aaaattggtc aatctttacc
    11521 aacactatta ccataaaaat cagaatattt tccttatggg agattcagca ggtggaggtt
    11581 tagccttagg tctagctcat gccctacaca atgaatcagt ccctcaacca aagcagctcg
    11641 ttcttttatc accttggtta gatgttacta tgtcacaccc agagattcca gaatacgaag
    11701 atgctgatcc tattctatct tcttggggat taaaacgagt gggagaattg tgggcttact
    11761 ctgctgataa taccaaccat atttatgtca gtcctaaaaa tggtccgatc acttatctac
    11821 caccaattac tttatttact gggacacgag agatctttta tcctgatatt cgcgattatg
    11881 ccgcaaaact gaaagccgct aaccacaata ttaccttcat tacccaagaa ggcatgaacc
    11941 acgtttaccc aatttatcct atcgaagagg caaaaacagc tcaataccaa atcattgatg
    12001 ccatcaacaa aactccttaa tgaggggaat aactcctacc tgccaccagt taatctggtg
    12061 gtttttttgt agtttgcacc acaaaataac aattaaaaag agccctatca taatgataag
    12121 gctctttctg tcataacatc cgcaccgcaa tacttaggat ttgttgtcaa gatctcgtaa
    12181 gagttgatca attttattac cataggcgat ggaattgtct ttttcaaaca ccaaatctgg
    12241 aattttgtac atggtcaatt gtttgccaag ttcacgttta atcgttccag tcgctttttc
    12301 aagccctgtt tgtgcttttt gattatcaga tgccaagtca ctcataatag tgtaataaac
    12361 tttagcaagt gacaagtcac cctgcatttg cacttctgta atcgttactc cctgaacgcg
    12421 tggatcacgc acttttttct gaagaatatc atttacttcg cgcttgattt ccatcccaac
    12481 gcggtcaata cgatgattag ccatagccat ttcctttcca catttttaaa acaaaagcta
    12541 ggctgaaagc ccagcttatg acattgttac ctgttttgat agcgacaatt aaagggaatc
    12601 tgagaattcc atgacgagac tggaaggaat gccccaagta gctcatctgg tagaaaaagt
    12661 cattatttcc gacagaggaa aaacgttaca tctcagacct tcttagcccc tattttctca
    12721 caatttcttc cataatatag gcttcaatag tatcgtcaac tttaagatcg ttaaagtttt
    12781 caatcatcaa cccaccttct tgagcattac cgacttcttt aacgtcatct ttgtaatgtt
    12841 tcaagctggc cagtttacca tcaaaaataa cgacactatc acggataaca cgaacactcg
    12901 aatcacgtgt caccttaccg ttgattacca taaagccacc aattgttccc actttagaaa
    12961 ctttgaaggt ttcacgaata atggcttcac caagaatttt ttcttggtaa acagggtcaa
    13021 gtttcccttt catggcttct tctacttctt caatgacttt atagataatg ctatgaagac
    13081 gaatttccac atcatccgta tctgcttgtt gacgagcttg tggggtaggg cgaacgttga
    13141 aaccgataat aacagcattt gatgcttcag caagggtcac atcagactca ttgatagcac
    13201 ctacagcaga gtgaacgaca ttgacacgga caccttccac ttcaatcttt acaagagatg
    13261 ctgcaagggc ttcaacagaa ccttgaacgt ctgctttgat gatgacatta acagtcttga
    13321 tttctcctgc tttaagggta tcaaagagat tatcaaggct aacacgctgt gtattttgac
    13381 gttgtttgag gagggcacgt tttgatcgct cctcaccagc tgcacgcgca gctttttcat
    13441 cagcgtaaac agcaaagtga tcaccagcca ttggtgtttc gttcaaacca gtaatggaaa
    13501 ctggtgttga tggctccgca gatttaacac gacgcccaag gtcatttacc atggcacgga
    13561 cacgaccaaa ggtattacca acaacgattg ggtcttgaac atgaagagtt ccttgttgga
    13621 caagaagtgt agcaatagca cctttacctt tatctaagcg tgcttcaatc acagtaccaa
    13681 tagcacgaac cgttggatca gctttcaatt cttctacttc agcaaccaaa aggactgttt
    13741 ctaatagttc atcaatattt ttattaaatt tagcagatat ctctacaaat tcgcactctc
    13801 cgccccaagc agttgagatg ataccatatt ctgccaattc tgcgataaca cgttctggat
    13861 tagcacctgg tttatcaatt ttgttgatag caacaataat tggaacacca gcagcttttg
    13921 agtgattgat cgcttcaatc gtttgtggca taacaccatc atcagctgca acaatcaaaa
    13981 tggtgatatc ggtaacggaa gctccacgtg cgcgcatgct tgtaaaggcc gcgtgtccgg
    14041 gagtatccaa gaaggtaatt ttcttgccag cttcttcaat ctggtaagca ccaatatgct
    14101 gagtaattcc tccagcttct cctgttgcca cacgtgagtt acgcaaagta tcaagcaaag
    14161 ttgtcttacc gtggtctacg tgtcccatga tagttacaac aggtgcgcgt tctacaatat
    14221 tttcaggatt gaggtaattt tcatcctcaa agaagcgctc gatatcagca tcatctactt
    14281 ctaccttagc cttagcctca ataccataat ctaccatcaa taattcgata gtatccccat
    14341 ctaaagactg gttttgagtt gccataacac ccatcataaa cagcttttta acaatttcag
    14401 cgggttcgcg tttgatacgt tttgcgattt cgacaactgt catgccttca gtatattcaa
    14461 attcttttgg caattcatgg aatttacgct cggttactgg ttttggagcg gtgttggtat
    14521 tcttaacatt tttgcctttt ttggtctttt tatttttatt ccagttacta tttttttgat
    14581 ttctcacttg gttttgacta ttccaactct tcttattttt cttttgtcct tcttgtgaat
    14641 gatgagttaa ctcacgattc ttttcaggac gtgcttgttt tttacggcga ttatcagcag
    14701 tagatttagc attaatattt gatgacttta atttagtttc tgcttcttca atagatttca
    14761 gtgctgcttc acgatggctc atctcctcag cttgtgcttt tagagccgct tctttagctt
    14821 cttgtcttgc caactcagct aaacgcttag cttcttgctg tgcacgaaaa cgtgtttcac
    14881 tttggcgtga gtattcagca ttttgttcag cttttaaggc agcagcacga gctttgaaat
    14941 caattttagg acttttcgat tgaggctctt gtcgtccttg ctttttttgg tttgtgaatc
    15001 gaccattctt gtgattctcc ttagcaaagg aacggttacg atcaccaact agctgatgat
    15061 tatcatgtct aggtgataca ttcgatgctt ttcgctcaat acgcttatcg tttgtatcac
    15121 cttgagaacg atgagtaatc ctcttatctc cttgttcgtt agtttgatga ttattgggct
    15181 ggcgataacc tcctcgacgt tcttgagaac gatgagaaga gccatttgct tgttttcgag
    15241 cagcctgttc ctttgcacga gcctctctct cagctttaaa attacgactc tttggcttag
    15301 agacctgtgt tttctctaca tgctcagaac ctgtttcttt gaccactgtt acagagggtt
    15361 gagcaacttc tttgggtttg actgtttgag aagctgtcac atcaggttta gaagcctttg
    15421 aaaaacttga aataatcttt ttcgcatccg cttcttcaac acttgatgca tggcttttca
    15481 catctaatcc taagtattta gcgtgttcaa ccacctcttt actactttta ccaatttcct
    15541 tggcaatttc atgtaatctt ttttttgaca atttgtgtcc tcctattcat ctattccata
    15601 agagtcctca ttttctttga aaatccagca tctgcaatgg cagcaacctt ccttggttta
    15661 cctagtgcag cacttaattc cagtgcattc aacactgtgg agacttctac attataataa
    15721 ttacttttat ctgttacttt ttttgtcaca ttaggaccag catcattggc taaaaatact
    15781 aatattacct gctggtgttg aatagctttt actactagtt cttcacctga aatcactttt
    15841 ccagcccgtt gagctagacc aataagacta gataatcttt ctaaattagt caagacctaa
    15901 ctctcttctt ttgattttgt gatcaacata agcaatcaag tcatcgtaaa aactttctgg
    15961 aatgtccatt gagaaactcc tgttgaaaac ttgttttttc ttggccatta aggcctcttg
    16021 attgtctaat ttgatataag caccacgacc attttgttta ccagttggat caatgaaaac
    16081 ttgaccatcc ttagttttta caatcctaag aagatctcgt ttagcaataa tttcaccaga
    16141 aacaagtgat tttcgtaaag gtattttttt gactttagac atcaaacacc tcttttctta
    16201 tactttctcg gcagtcataa catcgtcaac aactagctct tccacagctg ttgctgcttc
    16261 tttttccgcc tcaagacgat catattcgct agctgatttg atatcaatac ggtagccagt
    16321 caaatgtgcc gctaaacgaa cattttggcc gcgacgcccg atagctaatg ataatttgct
    16381 atcaggaact actaccgtag cacgtttaag atcttcatcg tcaaaaagta ccatgtctac
    16441 ttcagccgga gcaatggcat tgtagatgaa ctcagctgga tcatctaccc attgaatcac
    16501 gtcaatattt tcttcaactg gaatttcaag ccccgtctta gcatctacac gttttggatg
    16561 gaacttgctg atgacctttt taatattact tccaccacgt ccaacaattg ttccgatggc
    16621 atcaacgtta ggattatgac tgcgcacagc aaccttggtg cgatcacctg cttcacgaga
    16681 aacactcatg atttcaacag taccatcaaa gacttcagga atttcttgct ccataatacg
    16741 tttgatgaat tctggatggc tacgactaac aaagacgtta actcctttag gattattttc
    16801 aactttgtag acataaacgt caatacggtc atgtgatttg aaagtttcac ctggaatctg
    16861 gtcttggtga gacaattgtg cttctaagga accaagattg acataaataa aacgttgatc
    16921 aaaccgttca actgttcctg tcataatttc accttcatgc tctttgtatt cattgaacat
    16981 cacttcacgc atttggcggc gcatcttttc cataatagtc tgtttagctg attgagctgc
    17041 gacacgtcca aattcattga cagattcttc aaagcgaatc ttatcaccca attcataggc
    17101 agaactgata gcaagagcat ctttcaaact aatctcaagc cggctgtcaa aaacttcctc
    17161 aacaacctca cgaaccgtga atacttgaaa atcagctgtt ttttcattaa attcaataac
    17221 acatgattct gattgaccat agcgacgttt ataggcagac tttaatgatt ctgtcactgc
    17281 gtcaataata tctgctttat caatgtgttt ttcttcttcc aaaatacgga aggcttctag
    17341 catttctttg ctcataatgt tatctacctt gcttgtaaag cttggcaaaa atcctttctt
    17401 aaattaatac gatcaatagc cccctaaagg ctcccttaat tccttttgtt tagcttgtat
    17461 aaactctata ggtgttgttt caaatttgct taagcgctcc taaaaaagtt tagtgctttg
    17521 aaataaaagt tataatttga cagccattct caccttagca actgcttgat atggtatatt
    17581 cacgatctta tgacgtgttt tatcaagata atcaatggtc aacgtttcac catcaaaggc
    17641 taataagtct ccttgaaaaa ctttcacctt atcaatagct tgataaagac tcacattgat
    17701 ataagagcca actgctgctt ttaaactgtc agctgtctta agcggacgct ccaaacctgg
    17761 actagagact tctagcatat attggtttgg aaaaggatct ggatcaatcg tatctaacag
    17821 cggactaata atattcgtca agtcagatgt atcttccact gtgattccac cttccttatc
    17881 gactaaaata ctaaggatgt agtcactacc cattttgtca tactcaacgt ctactaattc
    17941 ataaggtgcc ttaatctcag gcgttactgt cttggttacg atatcgataa tagatgtatt
    18001 tgctattttg attacctcct ctatttttat gctcttctct aagataattg gtccttggga
    18061 atccacttaa tacaaaagtg gcgagacata tcccaccacc tttctgtatt atttaagcct
    18121 attataccat aatttaagag gaatgcaagg atatgcactt tgttttactc acaaggagag
    18181 gaggggattc gaacccccga gccccgttaa ggactacacg ctttccaagc gtgcgcacta
    18241 agccactatg cgacctctcc attactaaag aagttaatac tgtatcaact cctttatgtt
    18301 atcatatttt gaaacaaaat actactagaa atttgcctca actcggtaaa tgacttgacc
    18361 tttattagag aacttctctt catattctgt catgacattt ccttcatagt tactagcgtg
    18421 taagtcaagc cagatctgtc tcaaggtcat gccgtattga gaaaaactag ctaaactgta
    18481 ctcaaacaag ccccgattat cggttttaaa atgaatttca ccgtgttctg gtaagatacg
    18541 cttgtaagtg tctaagaaat ccttgtaggt caaacgacgt ttttcatgct tggtttttgg
    18601 ccaaggatct gaaaagttga ggtacatcat atccacctca ccatcttcaa aatagttggt
    18661 taaacttgat ccatccactc gcagaagttt gacattgggt acttctgaag ctaatacctt
    18721 atccaaggcg tagcttagga cagataattg aatatcaatg ccaatgtagt taatatcagg
    18781 atttttcaag gccatccctg tgataaatcc gccctttcct gaaccaactt cgatatgaat
    18841 aggacggtca tttccaaaaa cgtcatgcca acggcccttg gcatcttctg gatttaatat
    18901 cacatagtgt gggttattag ctaagtgctc ctcagcacct tttcgttttc taactcgcat
    18961 tatttctttc taaatatttc tctaaatttt ctgagggcat aaatctcctg attcacatgc
    19021 tccatgtcac gcttgtcaaa acacttgaga atttgtgtca ggtgagaaaa ttgaccatac
    19081 caaataattt tttgcataac cttgtcatta tttttatagc cataataaga cagccattct
    19141 gaccaacgag accgtggaat atagtggctc aacaggtaag caacatcata catccgatca
    19201 gtcagacgaa cagaatccca atctactaaa aaaatcatac cgctagtagt aatcacccaa
    19261 ttgctatgtt taatatctcc atgcacaatc gttgctactt ctgatttgaa ctctggtaag
    19321 ctccgtttta attctttaac gatagcttgt aagtatgaat tttgctgaat ttgcaagggt
    19381 gcattttgct caaaatcaac caataaatcg tatgggtttt caatcttata attgagctga
    19441 agcagttgat tgactaattt tttagatttg tgaaggcgca atagaatatg aatgatttgc
    19501 ttactgttca tatcttcttt ggtcaatgta cggccgttaa gccattcttg ggcactcatc
    19561 atatcaccat tgcccatgcg tttggcccaa agtaactgtg gcgcaatctg ttcttttgct
    19621 aaggcaggta gaataggggt cgtatttaat tttataaaga cacattcccc gtttggataa
    19681 gtgcctttat aagctttgcc acttttccca cgtaagggag tcaaggtaag ttcttgttcc
    19741 gtcgttgtca ccttatcgcc ctccttttct atcttttata gtcgtttacc cctattttac
    19801 ttgttttagg ctacttagtc aatgatcttc tttaacttat aaggtagata tatacaagct
    19861 atgaaaacca tacctacaat caaaatgagt gcttttggcg ctgatgagca acacagcata
    19921 ttgacgatta acattaaaaa gcttaaacct cgtaaaaaac ttaacacatt tttcttcttg
    19981 ctccgactat tttcaggata aagactagtc atataatggt aatcgtagtg ataataaagt
    20041 gaaagtaatt ggaatacaac taaatagtta aaaatgagtg ctaaacttac tgacaaataa
    20101 agattatgga taaaaaatac agataatgag cttaatagca ttagccgtaa aaataagccc
    20161 aaataatctg aactacgtaa aaaggctcta gcataaaggg acagccaaag gtttgaagga
    20221 gtttgcttaa ctaattttaa aagaggattt agataagttc tttctttcac ttttgtagaa
    20281 attcctttga ctgttgtaaa aagagaataa aactttaaaa tactttgctt gcgattgctt
    20341 tcaaatgcga cagcttttgc ccaatctaaa cgatttcctc tcaaaaaata tcttgtcttt
    20401 cgactaagca ctactctttt tagtgccaat aatatcaaaa ctaagataat aaaaataaat
    20461 aaagacagac ctagacgtct aaaaatagga tataaaaaga gcaatacagc agtctgcaaa
    20521 gttccccaaa gcaaaaaaga gagtcgttca gcttgtttga tataagaaat cacttcttct
    20581 tcttttggaa gtaagaaatg ctgatctgcc tcttctagat acgttgctat agatcccata
    20641 ctgagcaata atgggatcaa tatcccaaga caaacctgaa taggtagatg attcgttgga
    20701 aaatggttta gtagctgacc atactgcacc atagcaaagc ctaataaaaa catcaacacc
    20761 aaaacaaagt gatcatttaa gacgtaacga aggtatttat tttgctgttt ttgaaagtct
    20821 tgacggcgtt tcagaaataa tgctttcata cttaatcctc ttttgttaat gcaagataga
    20881 tatcatttaa gctcgctgaa cggtcaccaa aagcttcttg caagtcagca agagttcctt
    20941 gggcacgaac ttgtccatga tgcaaaatca caaaacgatc acacattctt tcagcagagt
    21001 ccaaaacatg tgtactcatc aaaatagatt ttccttttgc tttttcgact tctagcgttt
    21061 ggattaagtc tgaaatagct aaagggtcta accctaagaa tggttcatca agaataaata
    21121 gacttggatc aatgacaaag gcacaaataa tcatgacttt ttgtttcatc cctttagaaa
    21181 aattgacagg aaaccattca agtttgtcag ttaaacgaaa catttccaag aaaggttgtg
    21241 ctcgtttttg tgccacttct aagtcgatat cataagccat agctacggtg ttgatatgct
    21301 ctgataaggt caattcttca tagagactag gagtttctgg gataaaacct attttttgac
    21361 ggtaggcaac tgcattttct gctagagtta aaccatcaat agaaatactt ccttgataag
    21421 gcttcaaaaa gccaataatt tcgttgatag ttgttgactt tccagcacca tttagcccga
    21481 tcaaaccgac taactctcca ttgtctacag aaaatgagac atcgtttaaa acagggatat
    21541 tgtgatatcc tcctgttaga tttttaatgt ttaacatata atttctttct agtttttgat
    21601 atacttataa tatattataa caaattcaag tagagaaggt gatataaaat ggaaaattgt
    21661 attttttgca gcatcattca aggggatatc ccctcttcga aagtctacga agatgagcag
    21721 gtcctcgcct ttttagatat ctcacaaact acaaaagggc atactctagt cattcctaag
    21781 caacatgttc gtaacttact agagatgact gctgagacag ctagtcatct ctttgctcgt
    21841 attcctaaaa ttgctcgtgc tatccagtca gctactggcg ctactgccat gaatatcatt
    21901 aacaataacg aagccttagc gggacaaact gttttccatg ctcatgttca tctcgttcct
    21961 cgttataatg aagaagacgg tatctcaatt caatacacca ctcatgagcc tgactttcct
    22021 gtcttggaaa agctcgcccg acaaatcaat caggaggtta gttcatgaaa gttaaaacca
    22081 tgatagccgt cggattatta tcttttaccg cttacaaagc atatcaaaaa cgttgtacca
    22141 taaaagagct attatccatc agtcgacaag caaaggatgc tgcacaactt gatttagata
    22201 acattaaagc aaaccttgac ctcattcaca gtcaaggaaa agtaattcaa aatattagtc
    22261 aggacttggc ccataaatgg cgctacttta accaagagac acaagcacac ctcactgaaa
    22321 tccaaaatcg tatggccaaa tatcaaggag atagtgagct aatttctagt gaatcaccta
    22381 cttgccaaac cgtcatttta actaacaacc attccagata gtattatctc cctcatatac
    22441 tttatttgaa atcacagaca gctatcatct ttgatacctg tcttttttat attcaaaatc
    22501 ggatatttta aacataaaaa ccctcagaac gttaggtctg agggttgata ttagcaggag
    22561 gaactaatga tccagaccct gatgagctag actcatcagc tgctaaagca tctgatgaag
    22621 aagacggcgt agttggcgta tggcttgaag cagtagagtc ctggtctgta ctagcactct
    22681 gataagatga gccggtatct actccacttg aataattagc gtaagagcta tgactatcag
    22741 aataagatgg agcctggcct gaagagtcat agttgttgtt tactgtctga gacttagttg
    22801 acccatacaa attttcataa acagtagcat ttgtttttaa ttgactaacc ttatgaagtc
    22861 ctaattctgt tcggatacga ttttggattt ctaacaaatg attagaggta acaatttggt
    22921 atgatccacc atctgataaa gtggcatctt ctccttttag ttgataagtc ttaatagttc
    22981 taagtgcgtc acgataacct aatagactag ggatagtgcg agaagagatt tcgatattcg
    23041 tttgcatatt actacttaca gcagataaaa tcttccgata agagctaatg ctatcaagag
    23101 caaggatttt tttcaatacc ttttgaatga cttcacgttg acgcttttgt cgaccataat
    23161 ctccctcagg atcatcataa cgcatacgag cataaaccaa agcttgttca ccgttaattt
    23221 tgtgtgttcc aggcgcaaca gtagcttgat attcaggttc gttttcagca atcgagatag
    23281 gaaaatcaaa ctcatttgta actgtaatcc ctccaactgc attcacaaga tcaataaggc
    23341 cttgcatatt aatttgaaca tagttatcaa tggtgatatt caaaagatct tgcacggtca
    23401 taatagccat ctgagcgcca cctgctgcat aagcagcgtt aagcttagct tcaacaccat
    23461 tcatttcatt atttttgggt ccagataacg tggttaaggt atctcgttct aaactagtca
    23521 tagttgtttt cttggtcttt ggattaaccg taaccaaaat catcgaatca ctgtttcctt
    23581 cccacttgga ggcacgctct gaagagcctg tatcaacacc catcaacaag atagaaaaag
    23641 ctcttgtttg tttaatggca tcacttttct ttgaagatgt cgaaaaatct ttaaaggtct
    23701 ttgataattc tcctgttgag aaggtataag cactagttag atagacacct aatgccaaga
    23761 cagttgttaa cacaatagct gtgaacatta aaactatttt ttttccaatt ttcatcgatt
    23821 cacttactga cttacagatt tacgcatatg ataaacatct aagtcatctc cattttttga
    23881 tttgatgtcg ttttttcgca tactttcaat acgaaaacca tactttttat acaggtaaat
    23941 agccttagta tttcttactt gaacgtccaa cttaaggctt tcgatataag ggttttcttc
    24001 agcccaatca agagcaatct ctaacaataa ttgaccgata ccataaccac ggtaagtctt
    24061 agcaactaac ataaatacat ctgcttcagc ttggccttgg gataatactt ccccagaaag
    24121 atttaaaagc cctactactt tttcatctaa tctagctagc aggcaaaaat caataaaaga
    24181 ggttcgactt cttgataaaa agtctgacaa ttcttgagga gtagcctcta atatagtctc
    24241 tacgcctgtt ataaaatctg tttctttggc agctgccgtc actaaggaaa gtacagcttt
    24301 tgcatcttga ggtacgcctt cttcaataac gagactttgc tgaatcatgt gacattatct
    24361 ccagaagctg acgagagcgc tcaccatgcg ctaacaagtc tagttgacga cctttgtctc
    24421 gtttagtaat ggtaatttgc aaatagtctt gtaataatcc ctctcctaat aattctcccc
    24481 actcaataac ggtaacacca ttaccaaaaa gaaaatcatc taaatcaata gagtctggat
    24541 catcaccaac acggtaaata tctaagtgat aaagaggtag acgtccttca tattctctaa
    24601 caatagtata agttgggctt ttgatcattt gactaatccc catcccttta gcaatacctt
    24661 ttgccaatgt tgttttaccg gcacctaagt cgccagacaa tacaatcaca tctccaatag
    24721 acaaataagt acctagggtt tcgccgtaag ctttaagagt atattcattt tcactataaa
    24781 acatagcaac attatatcag aaaaaaaaca tacttccaag cactacctag aagatgactc
    24841 aaataaggta ttcgtaaagt aaaaacgctt ctttatatta aaggttaaaa tcaattaaag
    24901 aagcggcagt tagtagcaaa ataagtaaat tatttggtta taagatagtt aatatgatga
    24961 aatttacaat gaacaagaag gttgctcccc aaataatagg gtgaatatct tttgtttttc
    25021 cctcaacaac ttttactaga caatagaaaa taaaggcagc ggcaatacca taggaaatag
    25081 agtaacacag cgccataaag aaagctgcaa aaaaagctgg aagagcatct gcaaatttac
    25141 tccaattaac atcaagaaaa gaagacacca tcatgacacc cacaacaatt aaagctggag
    25201 ccgtagcagc agccgggaca atacctacaa gcggtaatag caatattgat aataagaagc
    25261 atactgcggt ggagactgct gttagtccag tacgtccacc ttcagcaatt cctgctgctg
    25321 attcaacata ggtagtcgta tttgaagttc caaccaaagc cccaatagaa gtaccgatag
    25381 catctgcaaa aagcgcacga tccatttttg aattaaagcc tatactattt tccaaagcat
    25441 tctcatcgtc ttgagagaaa atacctgttc gacgaccagt tccgataaag gtaccaattg
    25501 tgtcaaaagt atctgataga ctaaaagcaa aaatagtcat gaaaactagc ggtaaacgac
    25561 ttgaatcgct aaaaagagaa ctcaaaccat cgaaagctgc aaggaaagtt gtacctaatt
    25621 cagtccaagc ttcaccaata tggctgatcc caaaattgag gttggacaca tccactattc
    25681 ctaaaggaat acctacaaga gttgttgcga caataccaat taaaatagca cctctaaaat
    25741 ttcgaatcac aagagctgtc gttaaaagta aaccaaaaat agcaagtaat acactggaat
    25801 ccgtaaaact agaaatcgta ggtacaactc caccattggc gtctacaaat aatagaccat
    25861 ctgcaaatgt tttagccgat gctttagccg gttcaacacc atttaccatt actatatttt
    25921 cagcagagat agaaaaagta atgatatttg catttttaaa tcctaaataa gctacaaaga
    25981 caccaattcc cccaccaata gcatgctgta aactaactgg aatcgccttg atgatacttt
    26041 tacgaaactt ggttacggta ataaaaatat tgaataatcc acaaatgaaa accattgcca
    26101 aggcttcttg ccatgaaaac cctaaagcaa aaacaactgt ataagtgaaa aaagcgttaa
    26161 gtcccattcc tggtgccaac gcataaggca cattggcaaa taatcccata attaaggttg
    26221 atatggctgc tgcgataatc gtagccaaaa agacagcatt agagggcatc cccgctgcac
    26281 ctaaaatact tgggttaaca aacaaaatat aggacatggc aaaaaaggtc gttaaaccag
    26341 ccataatctc agttgagaca gttgtcccat tttcgcttaa cttaaaaaac ttttccattg
    26401 aaatggagtc tccttaatta aatagtaggt aaaatcaaaa agcgcttatc actagataag
    26461 tctttttgac acctcattat tattgttatt gttcgtatta aaatgaacaa taacactatg
    26521 ttaccatttt tttgttcact tttcaacgtc tataccttca tttattcatt aaaaaaacag
    26581 ttatcctatc ttttttacta atgaatacgc ttaacttatc ttttctcttt atcttggagc
    26641 aaaatttgct ataatctaat tatgactaaa aaattgattg ccattgattt ggatggaact
    26701 ttgttacatc atgacaacac catctctacc tatactcaaa aaaccatcaa agctgtccaa
    26761 gacaagggcc accacgtcat tatttcaaca ggccgtcctt accgcatggc actgggctac
    26821 tatctgcagc tcaatttaaa gaccccaatc atcactttta atggggcctt gacccacatg
    26881 cctgaacaaa aatgggccta tgaacataat gtcactttgg acaaaggcta cctactaagg
    26941 ttactaaaat atcaggatga tttccagatg gattttattg ccagtgagta tcgaaaaaat
    27001 gtctacatca ccatgaccaa tccagaaagt atcgacccgc aactctttgg tgttgatgaa
    27061 attactcagg acatggcatt agagattacc aaaatcactc gaaatccaaa cgccttattg
    27121 atgcaaacgc gccacgaaga caagtatgcc ttagctaaaa acatgcgtgc tttcttcaaa
    27181 gatgaaattg aaattgattc ttggggaggc cctctcaaca ttcttgaaat ttcgtctaag
    27241 aatatcaata aagcttacgc tctgaactat ctccttggaa tttacaatat ggataaaaag
    27301 gatttaatcg cctttggtga tgaacacaat gacactgaga tgctagcttt tgctggaact
    27361 ggttatgcca tgaaaaatgc tagccctgtg ctactgcctt atgctgatca gcagcttaat
    27421 ttttccaacg aagaagacgg tgttgccaaa aaattggaag aacttttctt atagtagcaa
    27481 acgaaaccac tatttttcag aaaatggtgg tttttcttct gtcaacagct atcaaaaaat
    27541 gaaaatcatt ttaataattt caacattttt cagcatattt ttgttttaaa cagcttaaaa
    27601 acacctttaa tatcaataat ctgcctattc aatcctaaat tctgaaaatt ttttcatgtt
    27661 ttgtactatt tttccgaaag g
//
LOCUS       DAUCLR010000018        27649 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.018, whole genome shotgun sequence.
ACCESSION   DAUCLR010000018 DAUCLR010000000
VERSION     DAUCLR010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27649
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.018"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(22..623)
                     /locus_tag="VGT87_000602"
                     /pseudo
     CDS             complement(22..623)
                     /locus_tag="VGT87_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014572446.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            908..1135
                     /locus_tag="VGT87_000603"
     CDS             908..1135
                     /locus_tag="VGT87_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEQ3813482.1"
                     /translation="MEIKKLETFHQMTIEKLAKVEGGKNNWQANVSGVIAAGSAGSPI
                     GFPVCGVACGYIGAKTAITLWAGVTGATGGF"
     gene            1148..1348
                     /locus_tag="VGT87_000604"
     CDS             1148..1348
                     /locus_tag="VGT87_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEQ3813483.1"
                     /translation="MTTMKELTINDMASISGGNTPGDAVIGGLGGLASGLKFCKLPHP
                     VLTGGCVVGFTVGGAYLGYTAN"
     gene            1887..2168
                     /locus_tag="VGT87_000605"
     CDS             1887..2168
                     /locus_tag="VGT87_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813484.1"
                     /translation="MKILDIIFNLSDRKMIFLENVEKLGGYIVPFTLIILSVAYNFRH
                     PYPIWAAVLGGISLIVIFIYFILIVYLYLRYRYLVWKKIPEIKINSTEL"
     gene            2362..2765
                     /locus_tag="VGT87_000606"
                     /pseudo
     CDS             2362..2765
                     /locus_tag="VGT87_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771275.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3016..4086
                     /locus_tag="VGT87_000607"
     CDS             3016..4086
                     /locus_tag="VGT87_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813485.1"
                     /translation="MMMLRDVKVKTSSLLVGCAATLLVSLSGTVAADSVHSSDRRFGV
                     GYKEETTSQFSSDQPSQVVLYRGEANTVRFTYTNQMYLMKDIRIALDGSDKSLTAQTV
                     PGMGHVYEGFQTSARGIFTMSGVPESTVPVANPNVQTKYIRYFKVIDDMHNTMYKGTV
                     FLVQPQAWKYTMKSVDQLPVDDLNHIGVAGIERMTTLIKNAGALLTTGGSGALPDNIK
                     VSINPKGGLATITYGAGSTDIIPPAVLWKKGSVKEPTEADQSVGTPTPGIPGKFKRDQ
                     SLNEHEAMVNVEPLSHVVKDNIKVIDEKSTGRFEPFRPNEDEKEKPASDVKVRPAEVG
                     SWLEPATALPSVEMSAEDRLKS"
     gene            4179..4577
                     /locus_tag="VGT87_000608"
     CDS             4179..4577
                     /locus_tag="VGT87_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEQ3813486.1"
                     /translation="MHRLPNRLIPEEDNNKLSRVAKEVVTEPRLNNPFLLPKKVLSLE
                     DDAAILNHLEEDRNVNKLFLEPVAAEAKREINFHPSDQQVTAKVKESSDFHQKQHQTG
                     ITYGDDTLADLAHHLLHSDGVIPSLNDKSA"
     gene            complement(4925..5194)
                     /gene="stcA"
                     /locus_tag="VGT87_000609"
     CDS             complement(4925..5194)
                     /gene="stcA"
                     /locus_tag="VGT87_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system protein StcA"
                     /protein_id="HEQ3813487.1"
                     /translation="MTKKQFYLILAISILCFILAVSPKPPIPKHTIERKRTAMTIGHS
                     KQNPHKKEALKHKKNKNKALFLPSGDSAIKSVISMTVRLLLDDQS"
     gene            complement(6108..6176)
                     /gene="shp2"
                     /locus_tag="VGT87_000610"
     CDS             complement(6108..6176)
                     /gene="shp2"
                     /locus_tag="VGT87_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone SHP2"
                     /protein_id="HEQ3813488.1"
                     /translation="MKKVNKALLFTLIMDILIIVGG"
     gene            6265..7131
                     /gene="rgg2"
                     /locus_tag="VGT87_000611"
     CDS             6265..7131
                     /gene="rgg2"
                     /locus_tag="VGT87_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg2"
                     /protein_id="HEQ3813489.1"
                     /translation="MEKELGKTLRRLRKGKQVSISFLADEYLSKSQISRFERGESEIT
                     CSRLLNLLDKLNITIDEFVSAHSKTHTHFFTLLSQARKCYAEKNVVKLTKLLKDYAHK
                     DYERTMIKAILFSIDSSIAPSQEELTRLTDYLFKVEQWGYYEIILLGNCSRFMNYNTL
                     FLLTKEMVASFAYSEQNKTNKMLVTQLSINCLIISIDHSCFEHSRYLINKIDLLLRDE
                     LNFYEKTVFLYVHGYYKLKQEEMSGEEDMRQALQIFKYLGEDSLYYSYKEHYRQIVLG
                     GKGDEDWSEADL"
     gene            7303..8130
                     /gene="mutM"
                     /locus_tag="VGT87_000612"
     CDS             7303..8130
                     /gene="mutM"
                     /locus_tag="VGT87_000612"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEQ3813490.1"
                     /translation="MPELPEVETVRRGLETLVLGQEIVAVTLKVPKMVKTDLETFALT
                     LPGQIIQSVGRRGKYLLIDLGQLVLVSHLRMEGKYLLFPDEVPDNKHFHVFFELKNGS
                     TLVYQDVRKFGTFDLIAKSQLSAFFAKRKLGPEPKKEIFKLKTFEAALLSSQKPIKPH
                     LLDQTLVAGLGNIYVDEVLWAAKVHPETASSRLNKAEIKRLHDETIRILALGIKKGGS
                     TVRTYRNALGADGTMQDYLQVYGQTGKSCPRCGQDIVKLKVGGRGTHICPKCQKKRP"
     gene            8127..8720
                     /locus_tag="VGT87_000613"
     CDS             8127..8720
                     /locus_tag="VGT87_000613"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEQ3813491.1"
                     /translation="MIIGITGGIASGKSTVVKVIRKAGYQVIDADQVVHDLQEKGGRL
                     YEALREAFGNQILKTDGELDRTKLSEMLFSNPDNMATSSAIQNQIIKEELAAKRDHLA
                     QSQAIFFMDIPLLMELGYQDWFDAIWLVYVDAQTQLQRLMARNRLDKGKARQRIASQL
                     PIEEKKPYASLVIDNSGDIAALIKQVQSALLSLANPR"
     gene            8910..10412
                     /locus_tag="VGT87_000614"
     CDS             8910..10412
                     /locus_tag="VGT87_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HEQ3813492.1"
                     /translation="MTTMTIAKGNQPIALQLGMLNRHGLIAGATGTGKTVTLKVLAEQ
                     LSLAGVPVFLADIKGDLSNLTKAGEVTDKLAARLATIGVSDYQPQAFPVRMWDVFGQN
                     GQPLRTTISELGPMMLSRLLNLNDTQTGVLNIVFKIADEKGWLLIDLKDLQAILKEVG
                     DHASDYSSHYGNVTKQSVGAIQRSLLTLEQEGAEQFFGEPALEVADLMQLDVASGYGA
                     INILSATKLFQSPTLYTTFLLWLLSELYELLPEVGDLDKPKMVFFFDEAHLLFKDAPK
                     VFLEKVEQIVRLIRSKGVGIFFVTQNPLDLPETVLAQLGNRIQHALRAYTPKEQKAVR
                     VAADTFRQNPDLDVARVITELEVGEALISVLNDKGQPSIVERAYIMPPKSSFAVLSDI
                     ESQQLVQSSPFASKYSQSIDRDSAYEKLAAKVLEDNRLAQEAIATAQREKEAKEAIKA
                     QAATKKASRRSVGRPRKTVVEKATDAFISTTVKTIGRELVRGLLGSLRKR"
     gene            10534..11727
                     /locus_tag="VGT87_000615"
     CDS             10534..11727
                     /locus_tag="VGT87_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEQ3813493.1"
                     /translation="MNWRQNLKVAWLGNFFTGASFSLVMPFMALYVENLGTPTELVEY
                     YAGLAVAVTALASALFAPVWGKLADRYGRKPMMLRASFVMTFTMGGLAIIPNVFWLLI
                     LRLLTGVFAGYVPNATALIASQAPKEESGYALGTLATGVTAGALIGPLLGGILAELLG
                     IRQVFLLVGVILFLCSLMTAVYVKEEFKPIRRFEMIPTKVILKQVKSPQIMLGLFVTS
                     MIIQISAQSVTPILSLYIRHLGQIHNLMFTSGLVVSAMGFSSLFSSSYLGKLGDRFGN
                     HRLLLAALCYSFIMYFSSALAQTSFQLGVLRFAYGFGVGALMPSINSLLTKLTPKEGI
                     SRVVAYNQMFSNLGQVIGPFIGSNVAVVLGYRSVFYVTSLIVFVNLIWSLIIFRKYIK
                     VKDIV"
     gene            11724..11870
                     /gene="rpmG"
                     /locus_tag="VGT87_000616"
     CDS             11724..11870
                     /gene="rpmG"
                     /locus_tag="VGT87_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEQ3813494.1"
                     /translation="MRVKINLECSECGSNNYLTSKNKSSHPEKIKVPKYCPKERKVTL
                     HVET"
     gene            11916..12152
                     /gene="secG"
                     /locus_tag="VGT87_000617"
     CDS             11916..12152
                     /gene="secG"
                     /locus_tag="VGT87_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEQ3813495.1"
                     /translation="MYNLLLTILLVLSVLLVIAIFMQPQKNPSSNVFDSSGSEALFER
                     TKARGFEAFMQRFTAVLVFFWLAIALVLAILSSK"
     gene            12246..14579
                     /gene="rnr"
                     /locus_tag="VGT87_000618"
     CDS             12246..14579
                     /gene="rnr"
                     /locus_tag="VGT87_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEQ3813496.1"
                     /translation="MMKERIMTYLKEHGKSNVNDLAAALEMAGAKHFPSLIKTISKME
                     SQSLLRFSDDGSLALRKEREKKKEPTVQGVFRANKAGFGFLHVDENEDDMFIGRNDVG
                     YAIDGDTVEVVVKKPADRLKGTAAEAKVVAIVDRSLKTAVGTFILDDDKPKYAGYIRS
                     KNQKIQQKIYIKKEPVVLKGTEIIKVDIDKYPTRGHDYFVASVRDIVGHQGDVGIDVL
                     EVLESMDIVSEFPAEVLAEANAISEAPTAKDLIGRVDLRQETTITIDGADAKDLDDAI
                     HIKLLDNGNYELGVHIADVSYYVTEGSSLDKEAIARGTSVYVTDRVVPMLPERLSNGI
                     CSLNPNVDRLTQSALMEINPQGYVVNYQICQSVIKTTYRMTYSTVNDMIAGDEEALQE
                     FASIADDVTLMVALHRILETMRSKRGALNFDTQEAKVIVNDKGMPVDVVLRQRGIAER
                     MIESFMLAANECVAEHFAKAKLPFIYRIHEEPKAEKLQQFIDYASTFGIHIQGTANKI
                     SQEALQAFMAKVEGQPGAEVLNMMLLRSMQQARYSEHNHGHYGLAAEYYTHFTSPIRR
                     YPDLLVHRMVREYSQPSQEKRDHFTQIIPELATSSSQLERRAIDAERVVEAMKKAEYM
                     AEYVGEEFDGIVASVVKFGFFVELPNTIEGLVHITSLPEYYHFNERTLSLQGEKSGKV
                     FKVGQPIRVKLVKADKETGDIDFEYLPSDFDVVEKIKMSDKASRRDRRKSSKSSKGIK
                     KKEPKEVAKAKTKGKTKKGSKKPFYKEQAKKKSRKRS"
     gene            14582..15049
                     /gene="smpB"
                     /locus_tag="VGT87_000619"
     CDS             14582..15049
                     /gene="smpB"
                     /locus_tag="VGT87_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEQ3813497.1"
                     /translation="MAKGEGHILAQNKKARHDYHIVETVEAGIVLTGTEIKSVRAARI
                     QLKDGFAQIKNGEAWLVNVHIAPFEQGNIWNADPERTRKLLLKKREITHLENELKGSG
                     MTLVPLKVYLKDGFAKVLIGLAKGKHEYDKRETIKRRDQERDIKKQMKHYNAR"
     gene            15055..15774
                     /locus_tag="VGT87_000620"
     CDS             15055..15774
                     /locus_tag="VGT87_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HEQ3813498.1"
                     /translation="MSIMITKGQVRLLRTYSYDSNLYTQGLEQLNNSHILLSAGRYGF
                     SKVGVYDLTQEIFSEKIAFPDTVFAEGLTVVEDYFWLLTYKEGVAYKFDKATCNCLGA
                     YPFEGDGWGLAYDKENQCLWMTSGNAFLQKRDPKDFALLDTVLVAIESVPISMLNELE
                     YVDGYLYANIWQTNTIVKLQPDSGKVVATYDISPLLKALNLDKSHYPDLNVLNGIAHL
                     DQQRFLITGKLYPLMLEVVLD"
     gene            complement(15891..16538)
                     /gene="pcp"
                     /locus_tag="VGT87_000621"
     CDS             complement(15891..16538)
                     /gene="pcp"
                     /locus_tag="VGT87_000621"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEQ3813499.1"
                     /translation="MKILVTGFDPFGGEAINPALEAIKKLPATIHGAEIKCIEVPTVF
                     QKSADVLQQHIESFQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RADGKAAYFSTLPIKAMVAAIHQAGLPASVSNTAGTFVCNHLMYQALYLVDKYCPNAK
                     AGFMHIPFMMEQVVDKPNTAAMNLDDITRGIEAAIFAIVDFKDRSDLKRVGGATH"
     gene            complement(16588..17514)
                     /locus_tag="VGT87_000622"
     CDS             complement(16588..17514)
                     /locus_tag="VGT87_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 family protein"
                     /protein_id="HEQ3813500.1"
                     /translation="MANIANLVLETIYILIGLQLFHTAYCTFKDKTNPVYFGTALFWG
                     LLGVTFVGGAFLPNKVIGFIVIVLALLTLFKQVRIGTLPAFNEQKAEEIAHRIGNWIF
                     LPVMLMAMISLLLALILPDFSKSAIGIAGILATIAILIITKQKPSALLAENNRMNQQV
                     STSGILPQLLGALGAIFAAAGVGDVIASLIREIVPADSRFFGVLAYVLGMVIFTMIMG
                     NAFAAFTVITTGIGVPFVFALGADPIIAGALAMTAGFCGTLLTPMAANFNALPVALME
                     IKDRNAVIKKQAPIALVLIISHIALMYLLAYY"
     gene            complement(17514..18197)
                     /locus_tag="VGT87_000623"
     CDS             complement(17514..18197)
                     /locus_tag="VGT87_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEQ3813501.1"
                     /translation="MEWIKLIGIVIIVLGFILKCDAIATVVVAGLVTALVSGISFIDF
                     LDILGKEFTNQRLLTIFFITLPLIGLSETYGLKHRATQLIQRVQSLTVGRLLTLYLII
                     RELAGLFSIRLGGHPQFVRPLIQPMGEAAAKANIGEELTDAEKDDIKAMAAANENFGN
                     FFAQNTFVGAGGVLLIAGTLEQLGYDGNQAKIAFSSILIAIISIIIVAIYNYLFEKKM
                     ERQHQKGDN"
     gene            complement(18406..19332)
                     /locus_tag="VGT87_000624"
     CDS             complement(18406..19332)
                     /locus_tag="VGT87_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEQ3813502.1"
                     /translation="MTLLSIIVPCFNEEANILPYFEEMHQLETSMTNQLAFEYIFIDD
                     GSKDNTLGILRELATRFPNVHYLSFSRHFGKEAGLLAGLKEAKGNYITVMDVDLQDPP
                     ELLPVMYAKLKEGYDIVGTRRQNRQGEPLIRSMCSNLFYGLIKHLSDTEMVNGVRDYR
                     LMTRQVVDSILELGEVNRFSKGIFSWVGYRITYLSFENQKRKYGKSRWHFWELLRYSL
                     DGFINFSEMPLTIATWTGTFSFLISIFAILFIIIRKILFGDPVSGWASTVSIILFMGG
                     IQLFCMGIIGKYISKIFLETKKRPLYIIKEKH"
     gene            complement(19477..19854)
                     /locus_tag="VGT87_000625"
     CDS             complement(19477..19854)
                     /locus_tag="VGT87_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEQ3813503.1"
                     /translation="MKALHTCIRVKDLDQSVAFYTSAFPFKENYRKDFPDSQFTLVYL
                     TLEGESYELELTYNYGHGDYDLGNGYGHIALGSEHFEADHKKHRQAGFPVTDIKELAD
                     KSARYYFIQDPDGYKIEVIDLNN"
     gene            complement(19865..20530)
                     /locus_tag="VGT87_000626"
     CDS             complement(19865..20530)
                     /locus_tag="VGT87_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEQ3813504.1"
                     /translation="MDQTIHHQIQQALHFRTAVRVYKEEKISDEDLALILDAAWLSPS
                     SVGLEGWRFVVLDNKPIKEEIKPFAWGAQYQLETASHFILLIAEKHARYDSPAIKNSL
                     LRRGIKEGDGLNSRLKLYESFQKEDMDMADNPRALFDWTAKQTYIALGNMMMTAALLG
                     IDTCPIEGFHYDKVNHILAKHNVIDLEKEGIASMLSLGYRLRDPKHAQVRKPKEEVIS
                     VVK"
     gene            complement(20579..21664)
                     /locus_tag="VGT87_000627"
     CDS             complement(20579..21664)
                     /locus_tag="VGT87_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEQ3813505.1"
                     /translation="MTKLDQIRLYLDQKGAELAIFSDPVTINYLTGFFCDPHERQLFL
                     FVYHDLAPVLFVPALEVARASQAISFPVFGYVDSENPWEKIKAVLPNTAAKTIYAEFD
                     HLNVNKFHGLQTIFSGQFNNLTPYVQGMRLIKSADEINKMMIAGQFADKAVQVGFDNI
                     SLDATETDVIAQIEFEMKKQGIHKMSFDTMVLTGNNAANPHGIPGTNNIENNALLLFD
                     LGVETLGYTSDMTRTVAVGQPDQFKIDIYNLCLEAQLVAIDFIKPGVTAAQVDAVARQ
                     VIEKSGYGEYFNHRLGHGIGMDVHEFPSIMASNDLVLEEGMCFSVEPGIYIPGKVGVR
                     IEDCGHVTKNGFEVFTHTPKELLYFEG"
     gene            21838..22839
                     /gene="ccpA"
                     /locus_tag="VGT87_000628"
     CDS             21838..22839
                     /gene="ccpA"
                     /locus_tag="VGT87_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002264971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEQ3813506.1"
                     /translation="MNTDDTITIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNIANSYFSILAKGIDDIAAMYKYNIVLASS
                     DEDDDKEVNVVNTLFAKQVDGIIFMGHHLTEKIRAEFSRSRTPVVLAGTVDLDHQLPS
                     VNIDYRAAVSNVVDILAENHKCIAFVSGPLIDDINGKVRLAGYKEGLKHNKLDFKEGL
                     VFEANYSYKEGFELAQRVINSGATAAYVAEDELAAGLLNGLFEAGKRVPEDFEIITSN
                     DSPVVQYTRPNLSSISQPVYDLGAVSMRMLTKIMNKEELEEKEILLNHGIKKRGTTK"
     gene            22969..23967
                     /locus_tag="VGT87_000629"
     CDS             22969..23967
                     /locus_tag="VGT87_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEQ3813507.1"
                     /translation="MKVLLYLEAENYLRKSGIGRAIKHQAKALSLVGQHFTTNPRETY
                     DLVHLNTYGLKSWLLMIKAQKAGKKVIMHGHSTEEDFRNSFIFSNLLSPWFKKYLCHF
                     YNKADAIITPTLYSKSLIESYGVKSPIFAVSNGIDLEQYGADPKKEAAFRRYFDIKEG
                     EKVVMGAGLFFLRKGIDDFVKVAQAMPDVRFIWFGETNKWVIPAQVRQMVNGNHPKNL
                     IFPGYIKGDVYEGAMTGADAFFFPSREETEGIVVLEALASRQHLVLRDIPVYYGWVDQ
                     SSAELATDIPGFIEALKKVFSGASNKVEAGYKVAQSRRLETVGHALVDVYKKVMEL"
     gene            23969..25303
                     /locus_tag="VGT87_000630"
     CDS             23969..25303
                     /locus_tag="VGT87_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEQ3813508.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKEELEKEGHEVYIFTTTDRD
                     VKRFEDPTIIRLPSVPFVSFTDRRVVYRGLISSYKIAKHYNLDIIHTQTEFSLGLLGK
                     MIGKALRIPVVHTYHTQYEDYVSYIANGKIIRPSMVKPLLRGYLKDLDGVICPSRIVL
                     NLLEGYEVTIPKRVIPTGIPLEKYIRDDITAEEVTNLKAELGIAGDETMLLSLSRISY
                     EKNIQAIINQMPAILAENAKIKLIIVGDGPYLQDLKHFAMQLEVDKHVTFTGMVPHDK
                     VALYYKACDFFISASTSETQGLTYIESLASGTPIIAHGNPYLDDVVTDKMFGTLYYAE
                     TDLTDAIIDAILKTPVMDKRLLAKKRYEISAQHFGKSIYTFYLDTLIARNSKEAQKLR
                     LYLNHSGKSSSLKLVQGAIHLPKRAAKVTAITSVKVVKAPIKLVHAIKDFLD"
     gene            25725..>27649
                     /gene="thrS"
                     /locus_tag="VGT87_000631"
     CDS             25725..>27649
                     /gene="thrS"
                     /locus_tag="VGT87_000631"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEQ3813509.1"
                     /translation="MIKITFPDGAVREFESGVTTFDIAESISKSLAKKALAGKFNDQL
                     IDTTRAIEEDGSIEIVTPDHKDAYEVLRHSAAHLFAQAAKRLFPNLHLGVGPAIAEGF
                     YYDTDNAEGQISNEDLPRIEAEMQKIVTENYPCIREEVTKEEALELFKDDPYKVELIN
                     EHAGAGLTVYRQGEFVDLCRGPHVPSTGRIQVFHLLNVAGAYWRGNSDNNMMQRIYGT
                     AWFDKKDLKAYLTRLEEAKERDHRKLGKELDLFMISQEVGQGLPFWLPDGATIRRTLE
                     RYITDKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPVMDMGDGEEFVLRPMNC
                     PHHIQVYKNHVRSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHIFVTPEQ
                     IQEEFQRALQLIIDVYADFNLTDYRFRLSYRDPNDTHKYYDNDEMWENAQSMLKAALD
                     EMGVDYFEAEGEAAFYGPKLDIQVKTALGNEETLSTIQLDFLLPERFDLKYIGADGEE
                     HRPVMIHRGVISTMERFTAILIETYKGAFPTWLAPHQVTVIPISNEAHIDYAWEVAKT
                     LRDRGVRADVDDRNEKMQYKIRASQTSKIPYQLIVGDKEMEDKSVNVRRYGSKATHTE
                     SVEEFVENILADIARKSR"
BASE COUNT         8525 a         4897 c         5925 g         8302 t
ORIGIN      
        1 agacatcgtt ctaaggcttc ttcatttgga aaaaccaccc tccttcttgc tttgtcgctt
       61 gatttcttta ttgagtgatt caatcaagtt tgtagagtaa atactagacc aaatgtaatg
      121 tggaaattga tagaaagtta ggagattttc cgttgtttca agtgatgtca taaccttttt
      181 gtaacgaggc ttccactcag aaacaagtgg tgaccataca tgcgctcaat aatgtcactg
      241 atttcgcgtg tcgtaacgcc agtctgatag agtttgatca ccatctcttc caagtggtca
      301 tctcgtctag cataagatgg taataaaact ggtgaaaact cgccgtttcg atctcttggg
      361 atgattaaat tgactaagcc atactttgtt tcaaactgac gagaataagt cccattacgg
      421 ctattacctg tgttgtaacc ttctttttca taaggttcat aaccaagaaa agctgatagt
      481 tcaacttgca agaggtcatt catagctatt tctaaagagg agcgaaagaa ttcatcaata
      541 tcttgttttt gtgctaggaa gttaagtaat tctgtggtaa actgagtcat gggaataaat
      601 ctctttctag tgaagtgtcg caactctact atacaggatt tattcctttt tgtgtgttta
      661 cacaagatat tttacactac catcaaaaat cttaaaaggt atttataaaa gcatgaatgg
      721 tatcatttaa acttcttaat taattaacta ttgtcacagc gtaagtgtag agaggataaa
      781 aagacatttc atgatgaaaa acagcctttt aagattttga aaccatgata ctgttcttta
      841 tgatattctg tactagtcga ggttgcaacg agacaaattt aagaattatg aaaagaagtt
      901 attcgatatg gagataaaga aactggaaac atttcaccaa atgactatag aaaagcttgc
      961 caaggttgag ggtggtaaga ataattggca ggcaaatgtc agtggggtta tcgctgcagg
     1021 tagtgctgga tctcctatag gttttccagt ttgtggtgta gcctgtggtt atattggagc
     1081 taaaacagct attactcttt gggctggtgt aacaggagct acaggcggat tctagaggaa
     1141 agaaaggatg acaacaatga aggaattaac aattaatgac atggctagta taagtggagg
     1201 aaacactcca ggagatgctg taattggtgg tcttggaggc ttagcttctg gattgaaatt
     1261 ttgtaaactg ccacatccag tattgacagg tggatgtgtt gtaggattta cagttggtgg
     1321 agcatatttg ggatatacag caaattaaat gatctatacg atttttatta ttatgaatgc
     1381 gtctataatg tttttaatca ttgttgatgc cattcataaa aaaatgttga taaatctata
     1441 ccatatttag tgtttttaat tttcaatcaa ttatcccttt cagaaaaatc ggtaaacatg
     1501 ttatctatcg tagctttggt agcgtcatta tttgttttta tcgataaaag aaaatagtga
     1561 ttatgccaat aaatttagat atcactctta gctttttaaa atacaaaaga gtgaaattaa
     1621 aatgaaaaag acttcaaaaa tatggaagtg ctaaaaaaat ggtctggcta tcataaatgg
     1681 aggcggaaaa gacggagcaa atatcttttt gacagggatg gcaggtgctg cgcagggagt
     1741 gacagtttgt gctcagacag gcgtatttat cccttggcaa ggttatattt tgtgtggagc
     1801 agctggtgct gctacaaata ttatctggcc tcactaaata tataaatgac tatctatcac
     1861 tgatgtataa gtagtagagg agtggtatga aaattttaga tattattttt aacctatctg
     1921 atagaaagat gatatttcta gaaaatgttg aaaagctggg aggttacatt gtccctttta
     1981 cccttatcat cttgagtgta gcctacaact ttaggcaccc ttatcctatt tgggctgctg
     2041 tactaggagg tatatccctt attgtgatct ttatttattt tatacttatt gtttatctat
     2101 atttgcgtta tagatacttg gtgtggaaaa aaataccaga aattaagatt aattctactg
     2161 agctataatg caatcacatt taaggggaaa atggatgact tatacaatat cagtacttat
     2221 gatatttaac tttagagact aaagagttaa aagcaaaatg cgtattaaaa attggttaaa
     2281 aaagttataa ctgtctctga taacaggaat aacaatttgt tcggttatat ttgctaccga
     2341 tagagtagag gaggtgaaga tatgcgtaat atattaagat ttttaggtat tgttattatt
     2401 tctgtgcagt gattctattt agtataggta gtttctacga tttaccatta atgaaaaata
     2461 tacaacttat ctgttggagt tttatccttg atttattcgt ttttgtctac aaacaaagac
     2521 aaacgacaga agtgctcaca tggtatcaag ttgttaagca attttggtta tttattaagt
     2581 gtactattct tattcctacc ttaattgctt ttattataat aataggatgt ttgacgagta
     2641 tatcagaggt atcaatttac ttttatttac accttattgt tgtttgctat acaataggaa
     2701 tgattttatc attaggtcgt cttatttctc ctaatcactc tatatttaat aaacttaaaa
     2761 agtgaagtga atcatattta aagtttgttt ttaaataatg ggactagttt agtgtaaccg
     2821 attcaattaa agaatagttc gtgacaagaa gatacctttg aagtttcaga aataatctgt
     2881 cttctatcct gctatactca tctttgccaa aactcgtcat tttatatgat ttatatagaa
     2941 ttacgagttt tttgttagac taatatgaga aaatatagta tgacgaattc ctaaataaaa
     3001 aataatgaaa ggaacatgat gatgttaaga gatgttaaag ttaaaacgag cagtttatta
     3061 gttggttgtg cggctacttt actagtcagt ttgagtggta cagttgcggc agattcagtc
     3121 cattcgtctg atagacgttt tggagttggc tataaggaag aaacaacctc tcagttttct
     3181 tcggatcagc cctcccaggt ggtattgtat cgaggtgagg ctaacacggt taggtttacc
     3241 tacaccaatc agatgtatct gatgaaagat attcgcattg ctttggatgg ttctgataag
     3301 tctttgaccg ctcagactgt tcctggtatg ggtcatgttt atgagggctt tcaaacttct
     3361 gctagaggga tttttacgat gtcaggagtt cctgaaagca ctgttcccgt tgctaaccct
     3421 aatgtacaaa ccaaatatat aaggtatttc aaagtcattg atgatatgca taacacaatg
     3481 tataaaggaa ctgtttttct tgttcaaccg caagcttgga aatacaccat gaaatctgtt
     3541 gatcagttac cagtagatga cttgaaccat attggcgttg ctggtattga acgaatgaca
     3601 actctcatta aaaatgcggg tgccctttta accacaggag gtagtggagc cttaccggac
     3661 aatattaaag tatctattaa tccaaagggg gggctggcca cgattactta tggggctggc
     3721 tctacggata ttattcctcc agcagtttta tggaaaaaag gctccgtaaa agagcctact
     3781 gaagccgatc aatctgtcgg aacaccgact cctggtattc ctggtaaatt caaacgagac
     3841 cagagcctta acgagcatga agctatggta aatgtcgaac cactgtctca tgtagtaaaa
     3901 gacaatataa aggtcataga tgaaaaatca acagggcggt ttgagccttt tagacctaat
     3961 gaagatgaga aggagaagcc tgccagcgat gttaaggtaa gaccagcaga agttggtagc
     4021 tggctagaac cagcgacagc tcttcctagt gttgaaatga gcgctgagga caggttaaaa
     4081 agttagttaa taatttgccg gttgctttag acaggcaaac aggaaagggc ttggaggttt
     4141 cttctgaagt agctgtgagc gcctcacagc cggaagaatt gcatcgattg cctaaccgtt
     4201 tgatacctga ggaagacaat aataaactat cacgagtagc caaggaagtc gttactgagc
     4261 caaggctaaa taaccccttt ttgttaccca agaaggttct ttcgttagaa gatgatgctg
     4321 ctatccttaa tcatttggag gaagaccgta atgtcaataa gttgttcctc gagccagttg
     4381 cggctgaagc caagagagaa attaattttc atccaagtga ccagcaggtg actgctaaag
     4441 ttaaggagtc atctgatttt catcagaaac agcaccaaac agggattact tatggagatg
     4501 atactctggc agatctggcg catcatttgc ttcattcaga tggtgttatt cctagtctaa
     4561 atgataagag tgcttaagaa ttatgtgcta gtaagtctcg ctaagaagag gagtgttaca
     4621 agataaataa agaagaggtc agggaactac cggcctcttt ttgtccgaaa tagcgagaat
     4681 gactctagct tacctgtatg taggtccgtg ctacacatct tgttaatgat agggagctgg
     4741 gatcagaaaa taattggtat atcgttttct cctaactttt taagaaagtg cgggcaaagt
     4801 gggctgttag ggtgatgggg ttgctctttc atgatcagtt ttttagcttg aagaaagcgt
     4861 ggtgatgaga ttagcttaaa aaagaacact accgaaatgg tagtgtccac taattttagg
     4921 agagctaaga ttggtcatct aacaggagtc taactgtcat gctaataaca gatttgatag
     4981 cgctgtcccc acttggaaga aatagagcct tatttttatt ttttttgtgt ttaagggctt
     5041 cttttttgtg agggttttgt tttgagtggc ctatggtcat cgcagtcctt ttcctttcaa
     5101 tagtgtgttt aggaattggt ggtttagggc tgacggcaag aataaaacac agtatgctaa
     5161 tagctaagat aagataaaac tgttttttgg tcattgttta ggcctttcta gttgaaaaat
     5221 gtagttgttg ttagtttagc ctttttagta gctggtattt aaaatagcat tagccaagtg
     5281 gttgtactgg ctatctaaac ggtactcagt attcctcacg tcttttccag caaggaaata
     5341 ccttgtggtg tatgctgaat cattccaggt agcatcaacg taggaattaa caccatgaat
     5401 ggtaacgatg tttcaagcat ggtgctcatt caatcctttt ttatcacata ccaagctgat
     5461 aagcctgcag cttctgccat atctttaaac acgacggcat aagcttgaca aacaccacac
     5521 ttgccacgat aaagagcttt aggagtatga ttgttgatgc tgcttacggt tttgcgattg
     5581 gtgctaagtt attttgtggc gtaagtgtat tcgttgatta aatggttata aataacctta
     5641 gccttgtcgt agtcactggt gatcggttta tctgtaatat ttttttgaca aaggttttat
     5701 attccttatg aagggctacc aaattgtctt taagagtata ggtaactgta atatcactgt
     5761 taaccatgat acgttggctt tgtctaccaa gtgtataagt actggtacga ctttgggaag
     5821 taaatgaagt aagggttcca taataatcac ctcattcgtc gagagtggca ccaggtcgtt
     5881 gctggttaat ggagctgata gtttgatagt aagtgttttg ttcagacaca tcataacggt
     5941 atgcccctgc tgtttcatca gctttgacct tgctgtcaat ggcagcaaac gcacttaaga
     6001 gtaaagctgc cgctaggtcg ctaacactct ttttaatatt tattagtttt cctgttacaa
     6061 actaaatata agggtttccc aaaagtggat tgtttttaag cgaaaggtta tccaccaacg
     6121 ataatcagga tatccatgat caatgtaaaa agcaaagctt tattaacttt tttcatgact
     6181 gtctcctttc tgattttcta ttttgtactt gtattatata atggatttag cagattttaa
     6241 ttttttgttg tgaaagtggg aaaaatggaa aaagaactcg gaaaaacctt aaggcgatta
     6301 cgtaagggta agcaggttag catttctttt ttagcagatg aatacctgtc caagtcacaa
     6361 atttcaagat ttgaacgagg ggaatcagaa atcacctgca gtcgcttgct taatcttttg
     6421 gataaactga atattacgat tgacgaattt gtttcggctc attccaagac gcatacgcat
     6481 tttttcacgc tcttaagtca ggcgagaaaa tgttatgctg agaaaaacgt ggttaaattg
     6541 accaaactgt tgaaggatta tgctcataaa gattatgaac gaacgatgat taaagctatt
     6601 cttttttcta ttgactcctc aattgcacct agtcaagaag aattgactcg cctcacagat
     6661 tatttattta aagtggaaca gtggggatat tatgagatca ttcttctggg gaattgctcg
     6721 cgcttcatga attacaatac cctattttta ttgactaagg aaatggtagc atcttttgct
     6781 tattcggaac aaaataaaac caataaaatg ttagtgactc agttatccat taattgtctc
     6841 attatcagta ttgaccattc ttgctttgaa catagtcgct atttgattaa caaaattgat
     6901 cttttgctgc gagacgagct caatttttat gaaaagactg ttttcttata tgtgcatggg
     6961 tattataaat taaagcagga agaaatgtct ggagaagagg acatgcggca ggccttgcag
     7021 atttttaagt atcttggaga ggactcattg tattatagtt ataaggaaca ttaccgccaa
     7081 atagtgctgg gaggtaaggg ggatgaggac tggtctgagg cagatttatg agttgctttc
     7141 tatcagatgt attcgtaaaa taagaaataa gaaaggagaa ggacagctgt gttttttgaa
     7201 acgcagtcag tagcctagac ctgagtctat tgagtgactt ttttatgctg agtttattct
     7261 ttgtagcagc aaagggtcta gctgtccttt aaataatgga ttatgccaga attaccagaa
     7321 gttgaaacgg tccgacgagg gcttgagact ttagtgcttg gccaagaaat tgtggctgtt
     7381 acgcttaaag tgccaaaaat ggttaaaacc gacttagaga cgtttgccct gaccctacct
     7441 ggtcaaatca ttcagtcagt tggccgtcgg ggaaaatatt tattaattga tttggggcaa
     7501 ttagtgttgg tatcacattt gcggatggaa ggcaaatatt tgctttttcc agatgaggtc
     7561 cctgataaca aacactttca tgtctttttt gaattgaaaa atggttcgac cttggtttac
     7621 caagatgtgc gtaaatttgg aacctttgat ttgatagcaa aatctcaatt atcagcgttt
     7681 tttgccaaac gaaaactagg ccctgaacca aagaaagaga tctttaagtt aaaaaccttt
     7741 gaggctgctt tgctatcctc ccaaaaaccc ataaaaccac atctgttaga ccagaccttg
     7801 gttgctggtt tagggaatat ttatgtggac gaggtacttt gggctgctaa ggttcatcca
     7861 gaaactgcgt cttctaggtt gaataaggcg gagataaaac ggctgcatga tgaaaccata
     7921 cgaatcctag ctttaggaat taaaaagggc ggttcgaccg tccgtactta ccgtaatgct
     7981 ttgggagctg atggcactat gcaggactat ttgcaggttt atggtcaaac tgggaaatcg
     8041 tgtcctcgtt gtggacagga tattgtcaaa ttaaaagttg gaggaagggg gacgcacatt
     8101 tgtccaaaat gccagaaaaa aagaccatga ttattggcat cacaggaggc attgcatcag
     8161 gaaaatcgac tgtggttaag gtcattagaa aagctggtta tcaggtgatt gatgcggatc
     8221 aagtggtcca tgacttgcaa gaaaaaggcg gtcgccttta cgaggctctt agagaagctt
     8281 ttgggaatca gattttaaaa acagatggtg agttagatcg cacaaagctg tcagaaatgc
     8341 ttttttcaaa cccagataat atggcaacat cctcagccat tcaaaatcaa atcattaagg
     8401 aagaattggc ggctaagcgt gaccacttag cacagagcca agccattttt ttcatggata
     8461 ttcccctttt aatggaattg ggctaccaag actggtttga tgcgatttgg ctggtttatg
     8521 tagatgctca gacacaattg cagcgcttaa tggctagaaa tcgtttagac aaaggaaaag
     8581 caaggcagcg aatagccagt cagttaccaa tagaggaaaa aaagccctat gccagtctag
     8641 tgattgataa tagtggggat attgcagcct taataaagca agtacaatct gcgttgttgt
     8701 cattagccaa tccgaggtag ctaagctgag agattcgtat catagctgtc aaaatcaagc
     8761 tatttctgtc taaaactagc tggtcccttt taaattagcg catcaagggc tatatgttat
     8821 aataatatta agagcgagac ccttctaaac actttaactt atgggtaaac acatatcgac
     8881 aagtatgatc atcaaaaaag aggaagccta tgactacaat gacaattgca aaaggcaatc
     8941 aaccgatagc cttacaatta gggatgctaa atcgtcacgg tttgattgct ggagcgactg
     9001 gaacaggtaa aactgtgacc ttaaaagtgc tagcagagca attaagttta gctggtgtgc
     9061 cagtcttttt ggcagatatc aaaggagacc tgtctaatct tactaaagca ggagaggtga
     9121 ctgataaatt ggctgcgcgt ttagccacaa taggggtaag tgattaccag cctcaggcct
     9181 ttcctgtaag aatgtgggat gtttttggtc aaaacggtca acccttacgg acaacgattt
     9241 ctgagctggg accgatgatg ttgtcgcgtt tattgaatct taatgatacc cagacaggtg
     9301 tcttaaacat cgtctttaaa atcgccgacg agaaaggttg gcttttgatt gatttgaaag
     9361 acttgcaggc cattctaaaa gaagttggtg accatgcctc agactattcc agccattacg
     9421 gtaatgtgac caaacaatct gtcggagcta tccaaagaag tctcttgact ttggaacaag
     9481 aaggggccga acaatttttt ggagagcctg ctttagaggt ggctgatttg atgcaattag
     9541 atgttgcttc gggttatgga gccattaaca ttttgtcagc gactaagctt ttccaatcac
     9601 ccacacttta cacgaccttc ttactttggt tattatcaga gctgtacgaa ttattgccag
     9661 aagttgggga tttggataaa cctaagatgg ttttcttttt tgatgaagct cacttgctct
     9721 tcaaagatgc ccctaaagtt ttcttggaaa aagttgaaca gattgtgcga cttattcgtt
     9781 caaagggagt tgggattttc tttgtgactc aaaacccact tgatttgcca gaaacggttt
     9841 tggctcagct tggaaatcgt attcagcatg ccttgcgcgc ttatacaccg aaagaacaaa
     9901 aggcggttcg tgtcgctgcg gatactttcc gacaaaatcc tgacttagat gtggcaaggg
     9961 tgattacaga actagaagtc ggcgaagctt tgatttctgt tttgaatgat aagggacaac
    10021 catccatcgt tgaacgtgcc tatatcatgc cgcctaagag tagctttgct gttttgtcag
    10081 atatagaaag tcagcagctt gtgcagtcct caccttttgc cagtaaatat agtcagtcca
    10141 ttgatcgtga ctctgcttat gaaaaattag ctgctaaggt tttagaagac aaccgactgg
    10201 cccaagaagc gatagcgaca gcacaacgcg aaaaagaagc taaagaagcc atcaaggcgc
    10261 aagcagctac caaaaaagct agtcgccgtt ctgttgggcg tccacgaaaa acagtcgtcg
    10321 aaaaagcaac agatgcgttt attagtacga cggttaaaac aattgggcga gaactggtca
    10381 gaggtttact gggtagttta cgtaaacgat gaaggctatc agatgagact tagcactatt
    10441 atgggatact gtttttggca gaaatgtgtt ataataatca aaggcccaat ggaaagaaga
    10501 tgatgatcta ttagtgataa agcggtaacg acagtgaact ggcgacaaaa tctaaaagtg
    10561 gcttggcttg gtaacttctt tactggggca agcttttcac ttgttatgcc ttttatggct
    10621 ctatatgtgg aaaatttagg tactccgaca gaattagtag aatactatgc tggtctggca
    10681 gttgctgtga cggcacttgc ttcagcttta tttgctcctg tttgggggaa gttggctgat
    10741 cgttatggcc gaaaacctat gatgctgcga gcaagttttg tcatgacctt taccatgggt
    10801 ggtttagcta ttattcctaa tgtgttttgg ttacttattt tgagattatt aacaggtgtc
    10861 tttgcgggct atgtgccaaa tgccactgcc ttgattgcca gccaagcccc aaaagaggag
    10921 tcaggttatg ctttaggcac attagcaaca ggagtgacag caggcgcttt aattggtcct
    10981 ttactagggg gaattttggc agagctatta ggaattcgac aagttttctt attagttggt
    11041 gtgatattat ttttatgttc tttaatgaca gctgtttatg ttaaagaaga gtttaagccg
    11101 attaggcgct ttgagatgat acctacaaaa gtcattttaa aacaggtaaa aagtccccag
    11161 attatgcttg gtttgtttgt gactagtatg attattcaaa tttcagctca atcagtgacc
    11221 cccattttat cattgtatat tcgtcatctt ggtcaaattc acaacttaat gtttacttca
    11281 ggcctggtag tttcggccat ggggttttct agcttattta gcagttctta cttaggaaaa
    11341 ttaggagacc gatttggcaa tcatcgatta ttgttagccg ctttgtgtta tagctttatc
    11401 atgtatttta gcagtgcctt agcgcagacc agttttcaat taggagtact acgctttgct
    11461 tatgggtttg gtgtgggcgc tttgatgcca agtattaact ctttactaac taagttaaca
    11521 ccaaaagaag gtatttctag agtagttgcc tataatcaaa tgtttagtaa ccttggtcaa
    11581 gtcattggcc cttttatcgg atcaaatgtg gctgtagtgt taggctaccg ttctgttttt
    11641 tatgtgacta gtttgattgt gtttgttaat ttaatatgga gtttgattat atttcgaaaa
    11701 tatattaaag ttaaggatat tgtgtgagag taaaaattaa tttagaatgc agtgagtgtg
    11761 gcagtaacaa ttatctaacg agtaaaaata aaagcagtca tccggaaaaa ataaaagttc
    11821 ccaaatactg ccccaaggag agaaaagtga ccctgcatgt tgaaacttga taagaattgt
    11881 gttagaatgg tcatgacaat agtggaggaa ataacatgta caacttacta cttacgatat
    11941 tgctagtact gtcggtattg cttgtgattg ctatttttat gcaacctcaa aaaaacccaa
    12001 gtagcaatgt ttttgatagc agcggttcag aagccttgtt tgaacgtaca aaagctcgtg
    12061 gctttgaagc attcatgcaa cgattcacag cagttctagt atttttttgg ctagcgattg
    12121 cacttgtgct tgccatttta tcaagtaaat aaggagtgag cttgacagag ttagtctagc
    12181 tcattttttt atcagcgaaa cggattgata tcctccaaca aacaaaacag aaatagaaat
    12241 taggtatgat gaaagaaaga attatgactt acctcaagga gcatggcaag tccaatgtga
    12301 acgacttggc tgcggccttg gagatggctg gagctaagca ttttcccagc cttattaaaa
    12361 ccatttccaa aatggaaagt cagagccttt tacggttttc agatgatggc agtctggctc
    12421 ttagaaaaga gcgtgaaaag aaaaaagaac ctactgttca gggtgttttt cgtgccaata
    12481 aagcaggctt tggtttctta catgtggatg agaatgaaga cgatatgttt atcggtcgta
    12541 atgatgttgg gtatgctatt gatggtgata ctgtcgaagt agtggttaaa aagccagcag
    12601 accgtctcaa aggcacagcg gctgaagcta aggtcgttgc tattgttgat cgttctttaa
    12661 aaacagctgt tggaaccttt attttagatg atgacaaacc aaaatatgct ggttatatca
    12721 ggtccaaaaa tcaaaagatt caacaaaaaa tctatatcaa aaaagagcct gttgtgctta
    12781 aaggcactga aatcatcaag gttgatattg ataaataccc gacacgcggg cacgattatt
    12841 ttgtagccag tgtgcgtgat atcgtaggcc atcaaggaga cgttggaatt gatgtcctag
    12901 aagtccttga atcgatggat atcgtgtcag agttcccagc agaagtttta gctgaggcca
    12961 atgctatttc agaagcacca actgctaaag atttaatagg ccgtgtggat ttgcgtcaag
    13021 aaaccaccat tactattgat ggtgcggacg ccaaagactt agatgatgct atccatatta
    13081 agctattgga taacggtaac tatgaacttg gggtacatat tgcggatgtc tcatattatg
    13141 tgacagaagg atcttccctt gataaggaag ctatcgcccg tggcacttct gtctatgtca
    13201 ccgatcgtgt ggttccgatg ttaccagagc gtttgtcaaa tggcatctgc tctttgaacc
    13261 ctaacgttga tcgtttgacc cagtcagcct taatggaaat taacccgcag gggtatgtgg
    13321 tcaattatca aatctgtcaa tcagtgatta agacaactta ccgaatgacg tatagtacgg
    13381 tcaatgacat gattgcagga gatgaagaag cgctccaaga attcgctagc attgctgatg
    13441 atgtgaccct catggtggct ttgcatagga ttttggaaac gatgcggtca aaacgaggcg
    13501 ccttgaattt tgatacgcaa gaagctaaag ttattgttaa tgacaaagga atgccggttg
    13561 atgtggtgct tcgtcaaagg ggaattgcag agcggatgat tgagtccttt atgctagcag
    13621 ccaatgagtg tgtggcagaa cattttgcaa aagctaagtt accgtttatc taccgtattc
    13681 acgaagaacc aaaggccgaa aaacttcaac aatttattga ttacgctagc acctttggta
    13741 ttcacattca aggaacagcc aacaagattt ctcaggaagc cttgcaagcc tttatggcaa
    13801 aagtcgaagg tcaaccagga gcagaagtgc ttaacatgat gctcttacgc tctatgcaac
    13861 aggcaagata ttctgaacat aatcatgggc attacggttt ggccgcagaa tattacactc
    13921 actttaccag tccgattcgt cgttaccctg acttgttggt tcaccgcatg gttcgtgagt
    13981 acagtcaacc tagtcaagaa aagcgcgacc attttactca gatcattcca gaattagcaa
    14041 catcttcttc acagttagag cgtcgtgcta ttgatgctga acgcgtggtt gaagccatga
    14101 aaaaagctga gtatatggca gagtatgtgg gtgaagaatt tgacggtatc gtggccagtg
    14161 tcgttaaatt tggttttttt gtagagttgc caaatacgat tgaaggtttg gttcatatta
    14221 ctagcttacc ggaatactat cactttaatg aaagaacgct aagccttcaa ggtgaaaaat
    14281 caggtaaagt ctttaaagtt ggtcaaccta ttcgtgtgaa attggttaag gctgacaagg
    14341 aaactggtga cattgacttt gagtatttac ctagcgactt tgatgtggtt gaaaaaatca
    14401 agatgtcgga taaagctagt cgacgagatc gccgtaaatc ctcaaaatca tctaaaggga
    14461 taaagaaaaa agaacctaaa gaagtagcca aggcaaaaac caagggtaaa actaaaaaag
    14521 ggtctaaaaa accattttac aaagaacaag caaaaaagaa gagtcgtaaa aggagttaac
    14581 tatggcaaaa ggtgaaggtc atatactggc gcaaaataaa aaggctagac atgattatca
    14641 cattgttgaa accgttgaag cagggattgt cttaacggga actgagatta aaagtgttcg
    14701 tgcagcccgt attcaactga aagatggctt tgcccaaatt aagaatgggg aagcgtggtt
    14761 ggtcaacgtg catattgcac cttttgaaca aggtaatatt tggaacgctg atcctgaacg
    14821 gacccgcaaa ctgttgctca aaaaacgtga gattacgcat ctagaaaatg aacttaaagg
    14881 atctggcatg accttagttc ctctcaaagt ttacctcaag gatggctttg ctaaagttct
    14941 gattggtcta gctaaaggga aacacgagta tgacaaacgc gaaaccatta aacgccgtga
    15001 tcaggaaaga gatattaaaa aacaaatgaa acactacaat gcaaggtaag gtgaatgagt
    15061 attatgataa ctaaaggaca agttcgtttg ttacgaactt attcttatga tagcaactta
    15121 tacacgcagg gattagaaca actcaacaac agtcatatat tacttagtgc cggtcgttat
    15181 ggtttctcaa aagtaggggt gtatgacctt actcaagaga tcttttcaga aaaaatagct
    15241 tttccagata cagtttttgc agaagggcta acagttgttg aggattattt ttggttacta
    15301 acctataaag agggtgtagc ctacaagttt gataaggcta cctgcaattg tttaggggcc
    15361 tatccatttg aaggagatgg ctggggatta gcctatgata aggaaaacca gtgtctttgg
    15421 atgactagtg gaaatgcttt tttgcagaag cgagacccca aagattttgc tttattggat
    15481 accgtactag ttgctataga aagtgtcccc atctcaatgc ttaacgaatt agagtatgta
    15541 gatggttatc tctatgccaa tatttggcaa accaatacta ttgtcaaact ccaacctgat
    15601 tctggaaagg tggtagccac ctatgatatt agtcctttgt taaaagcctt aaatcttgat
    15661 aagtcccatt accctgacct taatgttttg aatgggattg ctcatttaga tcaacaacgt
    15721 ttcttaatta ctggcaaact atacccgctg atgttagaag tagtattaga ttaagatgat
    15781 aattgagaaa gaccgatgaa aagtttccct aggtctttca tttttagcac aaaaaaaaca
    15841 gagttagttc atatctctgt ttttttaaaa taggtttagt agcgtcacag tcagtgagta
    15901 gcgcccccta cacgttttaa atcggaacga tctttgaaat cgacaatggc aaaaatagca
    15961 gcctcaattc ctcttgtaat atcatcgagg ttcatggcag ctgtattagg tttatcaaca
    16021 acctgttcca tcataaaggg aatatgcata aacccagctt tggcatttgg acaatattta
    16081 tccactaagt aaagggcttg atacatcaaa tgattgcaaa caaaggtacc agctgtatta
    16141 gaaacagaag caggaagccc agcctgatga atggcagcaa ccatcgcttt gattggcaag
    16201 gttgaaaaat aagctgcttt accatctgca cgaataggtg tatcaatagg ctgattccct
    16261 tcgttatcag gaatgcgagc atcgtcttga ttaatggcaa cgcgttctgg cgttagtcca
    16321 gtccggccac cagcttgccc aatacaaagg actgcatcag gttgaaagct ttcgatatgc
    16381 tgctggagca catcggcaga tttttgaaaa accgttggaa cttcaataca tttgatttct
    16441 gctccatgaa tggttgctgg caatttcttg atagcttcaa gggcaggatt aatagcttca
    16501 ccgccaaagg gatcaaagcc tgttacaaga attttcataa taatgtcctt tcaatgaata
    16561 actgacgcta ctaacttagc aaaaaagtta gtaataagca agtaaataca ttaaggcaat
    16621 gtgactaata attaaaacaa gggcgatagg agcttgcttt ttgataactg catttcgatc
    16681 tttgatctcc atcaaagcaa caggcaaggc attaaagttt gctgccattg gtgttaataa
    16741 ggtaccacaa aaaccagcag tcatagctaa agcaccggca ataatagggt cagctcctaa
    16801 agcaaaaaca aaaggaacac cgataccggt tgtaataacg gtaaaggcag caaaagcgtt
    16861 tcccataatc atagtaaaaa taaccatgcc aagcacataa gcgagaacac caaagaagcg
    16921 gctatcagca ggtacaatct cccgaatgag agaagcaatc acatcaccaa caccagcagc
    16981 tgcaaaaata gctcctaaag ctcctaagag ctgtggcaaa ataccgcttg tggacacttg
    17041 ttggttcatc cgattatttt cagctaaaag agcagatggt ttttgcttag tgattattaa
    17101 aatagcaata gtggctaaga taccagcaat cccaatggca cttttactga aatcaggaag
    17161 gatcaaggca agaagtagag aaatcatagc cattaacata actggtaaga aaatccagtt
    17221 accaatgcgg tgagcaattt cttcagcttt ttgttcatta aaggctggta atgttccaat
    17281 acgaacttgc ttaaacaagg ttaaaagagc taggacaatc acgataaaac cgataacttt
    17341 attaggcaaa aaggcaccgc caacgaaggt aacacccagc aatccccaga acagagctgt
    17401 cccaaaatag acagggttgg ttttatcttt aaaggtgcag taggctgtgt gaaacagttg
    17461 caagccgata aggatatata tcgtttctaa aacaaggttt gcaatgttag ccattagtta
    17521 tctcctttct gatgttgacg ttccattttt ttctcaaaga ggtaattgta aatagcaact
    17581 ataataatag agataatagc aattaaaata gaagaaaatg caattttagc ttggttacca
    17641 tcatagccaa gctgttctaa agttccagcg ataagtagga cacctcctgc gccaacaaag
    17701 gtattttggg caaagaaatt gccaaagttt tcattggcag ctgccatagc tttgatgtca
    17761 tctttttctg catcggtcaa ctcttctcca atgttggctt tagcagcagc ttctcccata
    17821 ggttgaatca aggggcgaac gaactgtgga tgtcccccta gtcgaatcga aaagagacct
    17881 gctaattctc gaataataag gtagagagtc aataaacggc cgacggtaag ggattgaaca
    17941 cgttgaatca actgagtagc tcggtgtttc agtccataag tttctgacaa gccgataagc
    18001 ggtaaagtga tgaagaaaat ggttagcaaa cgttgattgg taaattcttt tcctaaaata
    18061 tctagaaaat cgataaacga aattcctgaa accagcgcgg taacaagtcc tgcaacaact
    18121 acggtagcga tagcatcaca ttttaaaata aaccctaaca cgataatcac aataccgatt
    18181 aatttaatcc actccataaa gaactccttt ttcaatagta tgacgattat aacattagtc
    18241 gctaggattt gcaaaaaggc tcaacctaat agttcctttt ctacttaaag tgttaaggaa
    18301 ggtgatacta ttttggaaag gtcattatca tttggtataa cgggcttaaa aagaaagctt
    18361 cgcgcacaga aagtaggaaa cgattgatga gaaaaatgtt ttaatttaat gtttttcttt
    18421 gataatatag agaggccttt ttttagtttc aaggaatatt tttgaaatat attttccaat
    18481 aatacccatg caaaagagct gaatgcctcc catgaataaa ataatagaga cagtgctggc
    18541 ccaaccagag acaggatcac caaagagtat ctttctgata atgataaata agatggcgaa
    18601 aatagaaatc aaaaagctga aagtccctgt ccaggtagca atggttaacg gcatttctga
    18661 aaagttgata aagccatcta aagaatatcg gagcaactcc caaaaatgcc aacgactttt
    18721 tccatatttg cgtttttggt tttcaaaact aagataggtg atacgataac caacccacga
    18781 aaagatccct tttgaaaaac gattaacctc tcccaactca agaatgctat caacgacttg
    18841 tctggtcatt aaccgataat cccgaactcc attgaccatt tcagtatctg ataagtgttt
    18901 gatcagtcca taaaatagat tcgaacacat tgaacgaatt agtggttctc cttgtctatt
    18961 ttggcgcctg gtgccaacga tgtcataacc ctcttttagc ttagcgtaca taactggcaa
    19021 aagttcgggt ggatcttgca gatccacatc catcacagta atgtaattgc ctttggcttc
    19081 ttttagtcca gctaagagtc cagcttcctt gccaaaatgg cgtgaaaaag acagataatg
    19141 cacatttgga aagcgagtag ctagctcacg caaaataccc aaggtgttat ccttagaacc
    19201 atcatcaata aaaatgtatt caaaagcaag ttggttggtc atggacgttt ctagttgatg
    19261 catttcttca aaataaggaa gaatgtttgc ttcttcatta aagcagggga caatgataga
    19321 tagtagtgtc atagactttt ctttcaaatg gaattttagg gcaattgtaa cacaaaagct
    19381 gagatatttc aaaactctgt taataatgag attgtcccaa tcgtcaggtt aaaaaataaa
    19441 acaacagaag ctttaaagct tctgttgtta atagtattag ttgtttaaat caatgacttc
    19501 aattttgtag ccatctggat cctgaatgaa ataatagcgg gcagatttgt cagccagttc
    19561 tttgatatcg gtaactggaa aacctgcttg tcgatgtttt ttatggtctg cttcaaaatg
    19621 ttcagagcca agggcaatat gaccatagcc atttcctaag tcgtagtcgc catgaccata
    19681 attgtaggtt aattctaatt cataactttc tccttctaaa gtcagataaa caagagtaaa
    19741 ttgactatct ggaaaatctt tacgatagtt ttccttaaaa gggaaggcgc ttgtgtaaaa
    19801 agcaaccgac tggtcaaggt ctttgacgcg gatacaggta tgtaatgctt tcattttaat
    19861 ctccttattt aacgactgaa atcacttctt ctttaggctt acgaacttga gcatgtttgg
    19921 gatctcgcag gcgatagcct agggataaca tgctagcaat gccttctttt tctaaatcaa
    19981 tcacattatg cttagctagg atatgattga ccttatcata atgaaagcct tcaatagggc
    20041 aagtatcaat gcccaaaaga gcagctgtca tcatcatatt accaagagcg atataggttt
    20101 gtttagctgt ccaatcaaag agtgcccgag gattatctgc catatccatg tcctcttttt
    20161 ggaaagattc atagagtttt aggcggctgt tgagaccatc accttctttg ataccacgtc
    20221 gtaaaaggct atttttgata gcagggctat cgtatcttgc atgtttttca gctattaaaa
    20281 gaataaagtg actagctgtc tctagttgat actgggcccc ccaggcaaag ggcttgattt
    20341 cttctttaat aggcttgttg tctaaaacga caaagcgcca gccttctaag ccaacagaag
    20401 aagggcttaa ccaagcagca tcaaggataa gggctaaatc ctcatcagaa atcttttctt
    20461 ccttataaac acgaacggct gttctaaagt gtagtgcttg ctgtatttga tgatgaatgg
    20521 tttgatccat acgaatacct tcctttcgcg agggtttgtt taaccagatg gtaggggctt
    20581 agccttcaaa ataaagcaac tcttttggag tatgggtaaa gacttcaaag ccatttttag
    20641 tgacatggcc acagtcttcg atgcgcacac cgacctttcc tggaatgtaa atgcctggtt
    20701 caacagagaa gcacatacct tcttcaagga ccaagtcatt gctagccata atggagggaa
    20761 attcgtggac atccatgccg ataccatgtc ccaagcggtg gttgaagtac tcaccatatc
    20821 cagacttttc aatcacttga cgagcaacag catctacttg agcagctgtt acacctggtt
    20881 tgataaagtc aatagccact agttgtgcct ctaggcagag attgtagata tcgattttaa
    20941 attggtctgg ctgaccaaca gccacagttc tggtcatatc actggtgtag cctaaggtct
    21001 caacacctaa gtcaaaaaga agtagggcat tgttttcaat gttatttgtt ccaggaatgc
    21061 catggggatt ggctgcatta tttccagtta agaccatagt gtcaaagctc attttatgaa
    21121 tgccttgttt tttcatttca aactcaattt gagcgatgac gtctgtttcg gtagcatcta
    21181 aggaaatatt gtcaaaacca acttgaacag ccttatcagc gaactgcccg gcaatcatca
    21241 ttttattgat ttcatcagct gatttgatga ggcgcatgcc ttggacataa ggggttagat
    21301 tgttaaattg ccctgaaaag atagtctgca agccatggaa cttattgacg ttaaggtggt
    21361 caaattcagc ataaatggtt ttggctgctg tatttggtaa aacggccttg atcttttccc
    21421 aaggattttc agaatcaaca taaccaaaca caggaaatga tatggcctgg ctagctctgg
    21481 ccacctcaag ggcaggtaca aaaagcacgg gagctaaatc atggtagaca aacaagaaca
    21541 gttgtctttc atgtgggtca cagaagaatc cagttaaata gttaatcgta acaggatctg
    21601 agaaaatcgc caattcagct cccttttggt caagatacaa acgtatttgg tctaattttg
    21661 tcataggcta accttctttc tacttactat tttttcaaaa aaagcttaaa aaatcaagct
    21721 tcgagttaat ttttgaaaac tttttcaaaa attaacctat aacacttgaa agtggttaca
    21781 aatcatgcta aaatactttt tgaaagcgtc attttcaaat agaaaggaag ataaaaaatg
    21841 aatacagatg ataccattac aatttatgat gttgcccgtg aagctggtgt ttcaatggca
    21901 accgttagtc gtgttgttaa tggcaataaa aatgttaagg aaaatacacg taagaaagtt
    21961 ttagaagtta ttgatcgcct tgactatcgt cccaatgctg tcgcgcgtgg tctcgccagc
    22021 aaaaagacaa caaccgttgg cgttgtgatt ccaaatattg caaatagtta tttttctatc
    22081 ttagctaagg gtattgacga tatcgccgct atgtacaaat ataatattgt gcttgcctcc
    22141 agtgatgaag atgatgataa ggaggttaat gttgtcaata ccctttttgc caagcaagtg
    22201 gatggtatta tttttatggg gcatcacttg acagaaaaaa tccgggccga gttttcacgc
    22261 tctcgtactc cagttgtttt ggcaggaact gtagatcttg atcatcaatt accaagcgtc
    22321 aacattgact atagagccgc ggtgtcaaac gttgttgata ttttagctga aaatcataag
    22381 tgtatcgctt ttgtgtcagg accactcatt gatgatatca atggtaaagt gcgcttagca
    22441 gggtacaaag aagggttgaa gcacaataag cttgacttca aagaaggtct tgtttttgaa
    22501 gcgaactatt cttataaaga aggatttgaa ttggcgcagc gcgtcattaa ctcaggagca
    22561 acagcggctt atgtggctga agatgaattg gcagcaggcc ttttaaacgg cttgtttgaa
    22621 gcaggtaaac gcgtaccaga ggactttgaa atcatcacta gcaacgattc accagtggtt
    22681 caatacactc gtcccaattt gagttctatc agtcaacctg tttatgactt aggtgctgtt
    22741 agcatgcgga tgttgactaa aatcatgaac aaagaagagt tggaagaaaa agaaattctt
    22801 ttgaatcatg gtattaaaaa acgtgggaca actaagtaac tgtgacttat gagcaccatc
    22861 taacaaaagt atcacaatag actgtttctt caatactaaa atcagcctta gggctgattt
    22921 ttgcatgtct tagtgaaaaa tactataaaa tgagctataa tagaacttat gaaagtctta
    22981 ttgtatttag aagcagaaaa ttatctaaga aaatcaggaa ttggtcgagc gattaagcat
    23041 caggctaaag ccttgtcact tgttggtcaa cattttacga ctaatccaag agaaacttat
    23101 gatttggttc atctcaatac ctatggttta aaaagttggc tgctgatgat aaaagcacaa
    23161 aaagctggta agaaggttat catgcatggg cattctacag aagaagattt tagaaattct
    23221 tttatttttt caaatctatt atctccttgg tttaaaaaat acctttgtca cttttacaat
    23281 aaggcagatg ctatcattac ccctacccta tattctaagt ctttgattga gagttatgga
    23341 gtgaagtcac ctatttttgc agtgtcaaat gggattgatt tggagcagta cggagcagat
    23401 cctaaaaagg aagcagcttt tcgtcgctac tttgacatta aagagggtga aaaagtggtt
    23461 atgggagcag gattattttt tctgaggaaa ggaattgatg actttgtcaa agttgcccaa
    23521 gctatgccag atgttcgttt tatctggttt ggcgagacca acaaatgggt cattcctgct
    23581 caagttcgcc aaatggtcaa tggtaaccac ccgaaaaatc ttattttccc aggatacatt
    23641 aaaggggatg tttatgaagg tgccatgact ggtgcagatg cctttttctt tccaagtcgt
    23701 gaagaaacag aaggcattgt tgtcttagaa gccttggcca gtcgccagca ccttgtttta
    23761 cgtgatatac cagtttacta cggatgggtt gatcaaagta gtgcggaatt agcaaccgat
    23821 ataccaggtt ttatagaagc tctgaaaaaa gtcttttctg gtgccagcaa caaagttgaa
    23881 gctggttaca aggttgccca gagtcgtcgc ctagaaacgg ttggccatgc cttagtagat
    23941 gtctataaaa aagtaatgga gttataagat gcgtataggt ctattcacag atacctattt
    24001 tccacaagtt tcaggagtcg ctactagtat tcgtacgtta aaagaagagc tagaaaaaga
    24061 aggtcacgaa gtttatattt tcaccactac tgatagagat gtcaaacgct ttgaagaccc
    24121 gaccattatt cgactgccaa gtgttccttt tgtgtcattt acggatagac gtgtggttta
    24181 tcgtggcctc atttcgtcat acaaaattgc aaaacactat aatcttgata ttattcatac
    24241 gcaaactgag tttagcttag gcttattagg gaaaatgata ggcaaagctt tgcgaattcc
    24301 tgttgtccat acttaccata cccaatatga ggactacgtg agttatattg ccaacggaaa
    24361 aatcattcga ccaagtatgg tcaaacctct tcttaggggc tatttgaagg atttggatgg
    24421 ggttatctgc ccaagtagga ttgtcctcaa tcttctagaa ggttacgaag tgactatccc
    24481 taagcgggtt atcccaacag gcattccttt ggaaaaatat attcgtgatg acatcacagc
    24541 agaagaagta accaacttaa aagcagaatt gggcattgct ggtgatgaaa ccatgttatt
    24601 gagtttgtca cggatttctt atgaaaaaaa tattcaagct atcatcaatc agatgccagc
    24661 tattttggct gaaaatgcca agataaagct tattattgta ggagatggcc cctatttgca
    24721 agatttgaaa cactttgcga tgcagttaga ggttgacaaa cacgtgacct ttacaggcat
    24781 ggtgcctcat gataaggttg ctctgtacta taaggcttgt gatttcttta tctcagcatc
    24841 aactagtgag actcagggct tgacctatat tgaaagtttg gctagtggca ctcctattat
    24901 tgctcatggc aatccttatt tagatgatgt ggtgactgat aaaatgtttg gcactcttta
    24961 ttacgctgaa acagatttaa ctgatgctat tattgatgcc atactaaaaa caccagttat
    25021 ggataagcgg ttattagcaa aaaaacgtta tgaaatctca gcacagcact ttggaaaatc
    25081 tatttacacg ttctatttag atacgttaat tgctagaaat agcaaagaag ctcaaaagct
    25141 gagactttat cttaatcatt ctggtaaaag tagttctcta aaattagtgc aaggtgctat
    25201 tcacttgcct aaacgtgctg ctaaggtcac agctatcacc tcagtaaaag tagtcaaggc
    25261 tcctatcaag ctggtccatg ctatcaaaga ttttctggat taaggcttgt aaatttttga
    25321 aaacttgcta taataatctt gaagacaagt tagattggac agaatctttt aagcgagtgc
    25381 tggttggtgg aaagagcaca aggatagcta atctgatact ccttcctggt ttttatgcaa
    25441 acataaaagg gtggccccct tatcgccact agaggtgcaa ggcatgtttt ttgtcctgca
    25501 taaattaagg tggaaccacg ctgattgcgt cctttgggat gcggtcagtt tttttgtttt
    25561 ggaggggatt agccttgttt ttaaggacta gggagggtag attcgtgaat aaggtattgt
    25621 tcttctacgt ggtggtattg agctattcac taacctcttg tccctaattt gtcttactat
    25681 ccatgtttta gtggaaatct taactgactt aaaggagaaa gcaaatgatt aagattactt
    25741 ttccagatgg cgccgtgcgt gaatttgaat ctggcgtaac aacgtttgac attgctgagt
    25801 ccatcagcaa atcattggct aaaaaagctt tagctggtaa gttcaatgat caactcattg
    25861 acaccactcg tgccattgaa gaagatggca gtattgaaat cgtgacacct gatcacaaag
    25921 atgcttatga ggtgcttcgc cactctgcag cccatttatt tgcccaagct gcaaaacgct
    25981 tgttcccaaa tcttcatttg ggtgtgggcc ctgccattgc agaaggcttt tactatgata
    26041 cagataatgc tgaaggccaa atttcaaacg aagatttgcc acgtatcgaa gctgaaatgc
    26101 aaaaaatcgt gacagaaaat tacccatgta tccgtgaaga agtgaccaaa gaagaagctt
    26161 tggaattgtt caaagatgat ccatacaagg tagaattgat taatgagcat gctggtgctg
    26221 gcttgacggt ttaccgtcaa ggtgaatttg tggatctttg ccgtggccct catgtgccat
    26281 caacaggccg tatccaagtc ttccacttgc tcaatgtggc gggtgcttac tggcgtggta
    26341 acagcgacaa taacatgatg caacgtatct atggaacagc ttggtttgat aaaaaagacc
    26401 tcaaagctta cttgactcgt ctagaagaag ccaaagaacg tgatcaccgt aagcttggta
    26461 aagagcttga cttgttcatg attagtcaag aagttggtca aggcttgcca ttttggttac
    26521 cagatggagc gactattcgt cgtacacttg agcgctacat cacagataaa gaattagcat
    26581 caggctacca acacgtttac acaccaccac ttgcttctgt agaattgtac aagacatcag
    26641 gtcattggga tcattatcaa gaagatatgt tccctgtaat ggatatggga gatggtgagg
    26701 agtttgttct tcgtccaatg aactgcccac accacattca agtgtataaa aatcatgtgc
    26761 gttcttatcg tgaattgcct attcgtattg ccgagcttgg catgatgcac cgttacgaaa
    26821 aatcaggtgc cctatcagga cttcaacggg tacgtgaaat gaccttgaat gatggccaca
    26881 ttttcgtaac accagagcaa atccaggaag aatttcaacg tgcccttcaa ttaatcattg
    26941 atgtttatgc tgacttcaac ttgactgatt atcgttttcg tttgtcatac cgagacccta
    27001 acgatactca taaatattat gacaatgatg agatgtggga aaatgctcaa agtatgctta
    27061 aagctgcact tgatgagatg ggtgtagatt actttgaagc agaaggtgaa gcagcctttt
    27121 atgggcctaa gcttgatatt caggtgaaaa cagctcttgg taacgaagaa accctatcaa
    27181 ccattcagtt ggactttttg ttgccagaac gctttgacct taaatacatt ggtgcagatg
    27241 gcgaagaaca ccgcccagtc atgattcacc gtggtgttat ctcaactatg gaacgtttca
    27301 ctgctatcct tattgaaact tacaagggtg ctttcccaac ttggttggca cctcaccaag
    27361 taaccgtgat tccaatttca aatgaagccc acattgacta tgcttgggaa gtagcaaaaa
    27421 cccttcgtga ccgtggtgtt cgtgcagatg tcgatgaccg caatgaaaaa atgcagtaca
    27481 agattcgtgc gtctcaaacc agcaaaattc cttaccaatt aattgttggg gacaaagaaa
    27541 tggaagacaa atcagtcaat gttcgccgct atggtagtaa ggcgactcac actgaatctg
    27601 tggaagaatt tgtggaaaac atcttggcag acattgctcg caagtcacg
//
LOCUS       DAUCLR010000019        26677 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.019, whole genome shotgun sequence.
ACCESSION   DAUCLR010000019 DAUCLR010000000
VERSION     DAUCLR010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26677
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.019"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..669
                     /locus_tag="VGT87_000632"
     CDS             <1..669
                     /locus_tag="VGT87_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HEQ3813510.1"
                     /translation="LPLSKDYLNLEDAYLDDWRASFVPVSVKDCQKTNYTSLFLYHGD
                     IRYLAVDAIVNAANSELLGCFIPNHGCIDNAIHTFAGSRLRLACQAIMTEQGRKEAIG
                     QAKLTSAYHLPASYIIHTVGPRITKGHHVSPIRADLLARCYRSSLDLAVKAGLTSLAF
                     CSISTGEFGFPKKEAAQIAIKTVLKWQAEHPESKTLTIIFNTFTSEDKALYDTYLQKE
                     NNCE"
     gene            662..1543
                     /locus_tag="VGT87_000633"
     CDS             662..1543
                     /locus_tag="VGT87_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HEQ3813511.1"
                     /translation="MSNWTTYPQKNLTQAEQLAQLIKEADALVVGIGAGMSAADGFTY
                     IGPRFETAFPDFIAKYQFLDMLQASLFDFEDWQEYWAFQSRFVALNYLDQPVGQSYLD
                     LKEILETKDYHIITTNADNAFWVAGYDPHNIFHIQGEYGLWQCSQHCHQQTYKDDTVI
                     RQMIAEQKNMKVPGQLIPHCPECEAPFEINKRNEEKGMVEDADFHAQKARYEAFLSEH
                     KEGKVLYLEIGVGHTTPQFIKHPFWKRVSENPNALFVTLNHKHYRIPLSIRRQSLELT
                     EHIAQLISATKTIYQKS"
     gene            1590..2609
                     /locus_tag="VGT87_000634"
     CDS             1590..2609
                     /locus_tag="VGT87_000634"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEQ3813512.1"
                     /translation="MYLIEPIRNGKRITDGAVALAMQVYVQENLFLDDDILFPYYCDP
                     KVEIGKFQNAVVETNQEYLKEHHIPVVRRDTGGGAVYVDSGAVNICYLINDNGIFGDF
                     KRTYQPAIEALHHLGATEVEMSGRNDLVIDGKKVSGAAMTIANGRVYGGYSLLLDVDF
                     EAMEKALKPNRKKIESKGIRSVRSRVGNIREHLAPQYQGITIEEFKDLMVCQLLQIET
                     ISQAKRYDLTEKDWQQIDALTERKYHNWEWNYGNAPQYRYHRDGRFTGGTVDIHLDIK
                     KGYIAACRIYGDFFGKADIAELEGHLIGTRMEKEDVLATLNAIDLAPYLGAITAEELG
                     YLIFS"
     gene            2737..4407
                     /locus_tag="VGT87_000635"
     CDS             2737..4407
                     /locus_tag="VGT87_000635"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEQ3813513.1"
                     /translation="MKSDIEIAQSVALQPITDIVKKVGIDGDDIELYGKYKAKLSFEK
                     MKAVEANEPGKLILVTAINPTPAGEGKSTMSIGLADALNQMGKKTMLALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLQQGNDLGIDPRR
                     IIWKRVLDLNDRALRQVIVGLGSPVNGVPREDGFDITVASEIMAILCLATDLKDLKKR
                     LADIVVAYTYDRKPVYVRDLKVEGALTLILKDAIKPNLVQTIYGTPALIHGGPFANIA
                     HGCNSVLATSTALRLADYTVTEAGFGADLGAEKFLNIKVPNLPKAPDAIVIVATLRAL
                     KMHGGVAKSDLAAENCEAVRLGFANLKRHVENMRQFKVPVVVAINEFVADTEAEIATL
                     KALCEEIKVPVELASVWANGAEGGLALAKTVVRVIDQEAADYKRLYSDEDTLEEKVIN
                     IVTQIYGGKAVQFGPKAKTQLKQFAEFGWDKLPVCMAKTQYSFSDNPSLLGAPTDFDI
                     TIREFVPKTGAGFIVGLTGDVMTMPGLPKVPAAMAMDVAENGTALGLF"
     gene            4492..6069
                     /gene="cls"
                     /locus_tag="VGT87_000636"
     CDS             4492..6069
                     /gene="cls"
                     /locus_tag="VGT87_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEQ3813514.1"
                     /translation="MIIKKKAKVKYLLHKGKHGFLRGIFSRTTIIVLLIILQLVFLFQ
                     SYAWMEQYRVWITILESVFAITIVLYLVNSDMDAISRMTWLILIMIAPLLGSLFLIYT
                     KLDWGYRGLKQRINHLVDLSAPYLSDDDAILEVLKDSTSTTYHLVQYLERSRGNFPIY
                     NNTRVTYFPTGETFFDSLKEQLFLAKKYIFLEFFIIAEGQMWGEILSILEKKVSEGVE
                     VRVLFDGMNELSTLSSDYAKRLEQIGIKAKSFLPISPFISTYYNYRDHRKIVVIDGEV
                     SFTGGINLADEYINEVERFGRWKDAGLMLEGEATDSFLILFLQMWSITEKELIIDPYL
                     SDHSLKLPSDGYVIPYGDSPLDTDKIGKNVYIDILNHAKEYVYIMTPYLILDSEMEHA
                     LRFASERGVDIRIIMPGVPDKGVPYALAKTYYKALMTSGVKIYEYQPGFVHSKVFISD
                     NTKAVVGTINLDYRSLYHHFECATYLYRVSVIADIVNDFNEAQKQSLLMTSDHLKQRP
                     WYQKLIGLLVRTIAPLL"
     gene            6409..6747
                     /locus_tag="VGT87_000637"
     CDS             6409..6747
                     /locus_tag="VGT87_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813515.1"
                     /translation="MQIIKRDGHVADFDPDKIYQAIIKAARTVYVIDDTWRQNLTHVT
                     KKVVIDLEEAHVERPTINMVQSLVENRLMDAGYITIAEHYISYRLQRDLERNGYGDKI
                     VVHLRFEQIK"
     gene            complement(7218..8486)
                     /locus_tag="VGT87_000638"
     CDS             complement(7218..8486)
                     /locus_tag="VGT87_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEQ3813516.1"
                     /translation="MTTKYQTIISNIEQDIQKQRLKKGDKLPSIRVLSKVYHCSKDTV
                     QRALLELKYRHLIYAVPKSGYYVLGNVSMPDNVLNLSLEDYNNMAYEDFRLCLNEALS
                     AKDKYLFHYYHKTEGLEELREALLLYLAENSVYSNKDQLLITSGTQQALYILSQMPFP
                     NAGKTILLEKPTYHRMEAIVAQLGLPYQTISRHFNGLDLELLESLFQTGDIKFFYTIS
                     RFSHPLGLSYSTKEKEAIVRLAQRYQVYILEDDYLGDFVKLKEPPIHYYDTHHRIIYL
                     KSFSMSVFPALRIGALVLPSGLKPHFLTQKSLIDLDTNLLMQKALALYLENGMFQKNL
                     RFIKRYLKQRERQLALFLKQNCPDIHYQLTPTHLVIDYTTTDSYRNFTLDKSDRIIIT
                     GKKRYLSIAINQQIQSKLNSLIKNTCGKSN"
     gene            8576..9442
                     /locus_tag="VGT87_000639"
     CDS             8576..9442
                     /locus_tag="VGT87_000639"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HEQ3813517.1"
                     /translation="MKRIVVANDLVGVGKVALSASIPLMASCLLEQVLLPTCLLSSHT
                     GGGQSPVKHTTSSILDVFLTDWDNKRIKFDGIFIGYFTTTRDGQKLLEWLSRKQLPLI
                     LDPIMADNGKLYQGFSQKHIEIMIKLTQKAELVLPNISEACLLTQTAYLAKNYDEEDI
                     RQVARKLAKLGPKQVVISGISFEEDSIGFAYYQSDSDQLTFHFGKQFPYHFYGTGDIA
                     SAIITAGFYYQLPLTQILDLVVSFLEKVLQNTLTLKRDLILGVAYESHLNYLTKSFKK
                     LLEEKNVISKIS"
     gene            9420..9977
                     /locus_tag="VGT87_000640"
     CDS             9420..9977
                     /locus_tag="VGT87_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEQ3813518.1"
                     /translation="MSSQKLANYIELSMYAALIFISVQVLRIPVGMQFIHLGNALVVI
                     AVLIYGSKKGALVASLGLGIFDVLNGYASVVWITILESLAICLILHLVYERAMQSSKK
                     PKAIITITVIAALSKLTINLIKYLLLAYIGTQLPLSVSFWVALGKIGGSFGTSFVTLV
                     AVPLLYPVIKNLKEKVTIGQAKRGF"
     gene            10111..11655
                     /locus_tag="VGT87_000641"
     CDS             10111..11655
                     /locus_tag="VGT87_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEQ3813519.1"
                     /translation="MSILEVKQLSHGFGDRAIFENVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGHLQPDEGKVEWSKYVTAGYLDQHTVLESGQTVRDVLRTAFDELFKTENRIN
                     EIYASMADDKADIAVLMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMESDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNHLDAEHIEWLKRYLQHYENAFVLISHDI
                     SFLNDVINIVYHVENQSLVRYTGDYYQFQAVYEMKQSQLEAAYERQQKEIANLQDFVN
                     RNKARVATRNMAMSRQKKLDKMDIIELQAEKPKPNFEFKQARTPSRFIFQTKNLVIGY
                     DYPLTKEPLNITFERNQKIAIVGANGIGKSTLLKSLLGVIEPLEGHIVTGDFLEVGYF
                     EQEVTGINRQTPLEVVWDAFPALNQAEVRAALARCGLTSKHIESQIQVLSGGEQAKVR
                     FCLLMNRENNVLILDEPTNHLDIDAKNELKRALKAYKASILMVCHEPDFYNGWVTDTW
                     DFSKLT"
     gene            12020..13243
                     /locus_tag="VGT87_000642"
     CDS             12020..13243
                     /locus_tag="VGT87_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEQ3813520.1"
                     /translation="MTNLTFYAKIGISEEEHDSFVKEHQQISVLQGSDWAKIKNQWQN
                     ERIGIYKEEKQVASLSLLIKLLPLGRSIIYIPRGPVMDYLDRDLVAFTMKTLKDYGKT
                     KKALFIKYDPAILLKQYALGQEEEEKPLALAAIKNLQEAGVHWTGLTMEIADSIQPRF
                     QANIYTQENLEMQFPKHTRRLIKDAKQRGVETYRASQSELHKFSKIVSLTEKRKNISL
                     RNEAYFQKLMTTYGDRAYLHLAKVNIPQKLDQYRQQLNLINQDITRTQAHQKKRLKKL
                     EDQKASLERYITEFEGFTDQYPEEVVVAGILSISYGNVMEMLYAGMNDDFKKFYPQYL
                     LYPKVFQDAYQDGIIWANMGGVEGSLDDGLTKFKANFAPTIEEFIGEFNLPVSPLYHI
                     ANTMYKIRKQLKNKH"
     gene            complement(13285..14847)
                     /gene="guaA"
                     /locus_tag="VGT87_000643"
     CDS             complement(13285..14847)
                     /gene="guaA"
                     /locus_tag="VGT87_000643"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEQ3813521.1"
                     /translation="MTEISILNDVQKIIVLDYGSQYNQLIARRIREFGVFSELKSHKI
                     TAQELHEINPIGIVLSGGPNSVYADNAFGIDPEIFELGIPILGICYGMQLITHKLGGK
                     VVPAGQAGNREYGQSTLHLRETSKLFSGTPQEQLVLMSHGDAVTEIPEGFHLVGDSND
                     CPYAAIENTEKNLYGIQFHPEVRHSVYGNDILKNFAISICGARGDWSMDNFIDMEITK
                     IRETVGDRKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHGLLRKDEGDQVMGMLG
                     GKFGLNIIRVDASKRFLDLLADVEDPEKKRKIIGNEFVYVFDDEASKLKGVDFLAQGT
                     LYTDIIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRALGIALGMPEEI
                     VWRQPFPGPGLAIRVMGAITEEKLETVRESDAILREEIAKAGLDRDVWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAQLPWDVLKKISTRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            15014..16447
                     /locus_tag="VGT87_000644"
     CDS             15014..16447
                     /locus_tag="VGT87_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813522.1"
                     /translation="MLRKKRILGLFRPVELIFLGLLLSLVVSYLAWTNSFATLHNILA
                     TVGIVERSKDQQPRYHIGQAIQVQKSGPYHQWIGTINKQVEDIAENYRVSYHYEVVFP
                     IGKVTVSLPEHNLKKPDKPRFKKGDIVKLSSLTKKPHIKVYQGQLATIKQVKKRYDYS
                     LGGYQYDINLKDNLRLDGISEQDFVKPYYIRFNKGNSPEQNNRLLRKAFAYAKQHPNS
                     VISFPKGQFHIGSLPSQKDYFELPSDTAIIGHQTEFIIHGKMLWFGFPTGPKAEQGVR
                     NLVLTGVHFKANDLKKGDHFMIMADHGTDWHIYDNKFTMVHKRNSHIFDLGSLQNSLF
                     EKNQFIGYAPELVQDQQLLSKAQGHDFFSEVIQFDAAVHHFAWDGGLLSNIAPNYEAF
                     NQTRHLCHNITVSQNQFLPYIDPTGCLRAYSGSIGQHSSKVGVIRVLNNVFTSSIVTK
                     AKLTSWFMEPIHFPPNSPVIVAGNIIN"
     gene            16530..17228
                     /locus_tag="VGT87_000645"
     CDS             16530..17228
                     /locus_tag="VGT87_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ3813523.1"
                     /translation="MLPAYIKIHDAIKKDIDLGIWPIGSRLPSERHLAEHFTVSRMTL
                     RQAITLLVEEGILERRIGSGTYVASHRVQEKMRGTTSFTEIIRSQGRQPSSKLLSYQK
                     QLASDTEVKELNLDKTDLVIRMERIRYADSVPLVYEIASIPEKFIKTVKRADITEHFF
                     HSLIANGYEIGKSKQTIYAKLASERVASYLEVAKGHAILALTQVSYFTDGKPFEYVRS
                     QYIGDRFEFYLENN"
     gene            17317..17658
                     /locus_tag="VGT87_000646"
     CDS             17317..17658
                     /locus_tag="VGT87_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEQ3813524.1"
                     /translation="MNIMEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEI
                     ADEFGVSRQAVYDNIKRTEKILETYEMKLHMYSDYVVRSEIFDDMIAHYPHDEYLQEK
                     ISILTSIDNRE"
     gene            17673..19235
                     /gene="ffh"
                     /locus_tag="VGT87_000647"
     CDS             17673..19235
                     /gene="ffh"
                     /locus_tag="VGT87_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEQ3813525.1"
                     /translation="MAFESLTQRLQDVFKHIRGKKKLSESDVQEVTKEIRLALLEADV
                     ALPVVKTFIKRVRERAIGHEIIDTLDPTQQILKIVNEELTSILGSETAEIDKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLIKEENARPLMIAADIYRPAAIDQLKTLGQQINVPV
                     FDMGTDHSAVDIVRKGLEQARENRNDYVLIDTAGRLQIDEKLMEELRDVKALAQPNEI
                     LLVVDSMIGQEAANVAYEFNHQLSITGVVLTKIDGDTRGGAALSVREITGKPIKFTGI
                     GEKITDIETFHPDRMSSRILGMGDLLTLIEKASQEYDEKKSLELAEKMRENTFDFNDF
                     IEQLDQVQNMGPMEDLLKMIPGMAGNPALANIKVDENQIARKRAIVSSMTPAERENPD
                     LLNPSRRRRIAAGSGNSFVEVNKFIKDFNQAKSMMQGVMSGDMSKMMKDMGINPNNLP
                     KNMPAGMPDMSSLEGMMGQGGMPDLSGLGGDMDMSQLFGKGFKGKIGQFAMKQAMKRQ
                     ANKLKKAKKKRK"
     gene            complement(19370..20068)
                     /locus_tag="VGT87_000648"
     CDS             complement(19370..20068)
                     /locus_tag="VGT87_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ3813526.1"
                     /translation="MFSGHQLKTARLSRGITQSELGRLLHVNKMTISNWEKGKNIPNE
                     KHLNALLHLFNVTSDYFDPNYRLLTPYNQLTISNKEKVIGYSERLLNHQIDKKSKDLI
                     DKPSQLYAYRVYESLSAGTGYSYFGDGNFDVVFYDEQLEYDFASWVFGDSMEPTYLNG
                     EVVLIKQNSFDYDGAIYAVEWDGQTYIKKVFREDEGLRLVSLNKKYSDKFAPYSEEPR
                     IIGKIIANFRPLEI"
     gene            20538..21608
                     /gene="xerS"
                     /locus_tag="VGT87_000649"
     CDS             20538..21608
                     /gene="xerS"
                     /locus_tag="VGT87_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEQ3813527.1"
                     /translation="MRRELLLEKIETYKAIMPWYVLDYYQSKLAVPYSFTTLYEYLKE
                     YKRFFDWLMDADLTQAPKIADIDLSTLEHLTKKDLEAFVLYLRERPSLNTYSTKEGLS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLASRAENIKQK
                     LFLGDETLAFLDYVDKEYEQKLSNRAKSSFRKNKERDLAIISLLLASGVRLSEAVNLD
                     LKDVNLNMMIIEVIRKGGKRDSVNVAGFAKGYLESYLAVRQRRYKAEKQDLAFFLTEY
                     RGVPNRMDASSIEKMVGKYSEDFKIRVTPHKLRHTLATRLYDATKSQVLVSHQLGHSS
                     TQVTDLYTHIVNDEQKNALDNL"
     gene            22308..22790
                     /locus_tag="VGT87_000650"
     CDS             22308..22790
                     /locus_tag="VGT87_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEQ3813528.1"
                     /translation="MPKRIRKALGVVGKLMSIVPDTTEIIGKTIDNSRPIIEKRMEQK
                     HEKEMQLRTIDDVINLTVDQAQAHLEQLGFVVATIPAKPHKKWLHSNLNEVVAMSPKS
                     GKYKIGSLIKLYYITVDVLEKSQDLLDQENLRTVERNQKIADTFESVKHIRFPFKKWR
                     "
     gene            22879..23931
                     /gene="citC"
                     /locus_tag="VGT87_000651"
     CDS             22879..23931
                     /gene="citC"
                     /locus_tag="VGT87_000651"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HEQ3813529.1"
                     /translation="MPYYTISKVFPSDKTTMASVKNLLHQEGIRLDTHLDYTCAIMNA
                     QNDVIATGSYFGNSLRCLCVSSAYQGEGLLNRIVSHLIDEEYALGNYHLFVYTKTSSA
                     AFFKDLGFTEIVHIDNHISFLENKKTGFQDYLMTLNKPEQTPGKVAAIVINANPFTLG
                     HQFLVEKAARENDWVHLFMVSEDRSLIPFSVRKRLIQEGLAHLDNVIYHETGPYLISQ
                     ATFPAYFQKEDNDVIKSQALLDTAIFLKIAQTLQITKRYVGEEPTSRVTAIYNEIMAE
                     QLQQAGILLDILPRKAINQQQDPISASTARQALKDNDWELLAKLLPKTSLDYFCSLEA
                     QPIIKKIQATSSVKHY"
     gene            complement(24041..25435)
                     /locus_tag="VGT87_000652"
     CDS             complement(24041..25435)
                     /locus_tag="VGT87_000652"
                     /EC_number="4.1.1.112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HEQ3813530.1"
                     /translation="MTKIRITETVLRDGQQSQIATRMTTKEMIPILETLDNAGYHALE
                     MWGGATFDSCLRFLNEDPWERLRAIRKAVKKTKLQMLLRGQNLLGYRNYADDVVRSFV
                     QKSIENGIDIVRIFDALNDPRNLQTAVSATKKFGGHAQVAISYTTSPVYTIDYFVELA
                     KAYQAIGADSICIKDMAGVLTPEIGYQLVKCIKENTTIPLEVHTHATSGISEMTYLKV
                     AEAGADIIDTAISSFSGGTSQPATESMAIALTDLGFDTGLDMQEVAKVAEYFNTIRDH
                     YRETGILNPKVKDTEPKTLIYQVPGGMLSNLLSQLTEQGLADKYEEVLAEVPKVRADL
                     GYPPLVTPLSQMVGTQALMNIISGERYKVVPNEIKDYVRGLYGQSPAPLAEGIKEKII
                     GDEVVITCRPADLIEPQMIYLRDEIAPYARSEEDVLSYASFPQQARDFLGRREDPFYD
                     VPVQEVTVQLDIQD"
     gene            complement(25450..26028)
                     /gene="citX"
                     /locus_tag="VGT87_000653"
     CDS             complement(25450..26028)
                     /gene="citX"
                     /locus_tag="VGT87_000653"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HEQ3813531.1"
                     /translation="MCKDTYFSGEAIQLSDMLRAREERALRQLHLLKEYPEGSLLSVT
                     MNIPGPIKTSPKLLEAFDIVIKAIQTALADDKICYQLRLLPTTGYEYYLITSLPSRDL
                     KLKMIALETELPIGRLMDLDVLVLQNDLPHSISRTVLGGSPRQCFICSKEAKVCGRLR
                     KHSVEEMQTAISKLLHSFFNKDNQSSSSDKTG"
     gene            complement(26021..>26677)
                     /locus_tag="VGT87_000654"
     CDS             complement(26021..>26677)
                     /locus_tag="VGT87_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407592.1"
                     /note="citrate-ACP transferase, the alpha subunit
                     catalyzes the formation of (3S)-citryl-CoA from acetyl-CoA
                     and citrate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HEQ3813532.1"
                     /translation="EAMIKENIKASFALGGITNAMVELLEEGLVEKILDVQDFDHPSA
                     VSLGKHAEHYEIDANMYASPLSKGAVINQLDTCILSALEVDTNFNVNVMTGSDGVIRG
                     ASGGHCDTAFAAKMSLVISPLIRGRIPTFVDEVNTVITPGTSVDVVVTEVGIAINPNR
                     QDLVDHFKSLNVPQFSIEELKEKAYAIVGTPERIQYGDKVVALIEYRDGSLMDVVYNV
                     "
BASE COUNT         8524 a         4607 c         5214 g         8332 t
ORIGIN      
        1 ttgcccttat caaaagatta cttgaatctg gaagatgctt atctagatga ttggcgtgct
       61 tcttttgttc ctgtatcagt caaggattgc caaaaaacta attataccag tctttttctt
      121 taccatgggg atattaggta ccttgctgtg gatgctattg tcaatgctgc taatagtgag
      181 ttattgggat gttttattcc taaccatggc tgtattgata atgcgattca tacgtttgcc
      241 ggaagtcgtt tacgcttagc ttgtcaggca atcatgactg aacaaggccg taaggaagcc
      301 attggacaag caaagcttac cagtgcttat catctaccag cctcttatat tattcatacc
      361 gtagggccaa gaattaccaa aggacaccac gtttctccga ttcgagctga tttgttagca
      421 cgttgttatc gttctagtct tgatttggcg gttaaagcag gattgactag cctagctttt
      481 tgctctattt caacaggaga atttggcttt cctaaaaaag aagccgctca gattgctatt
      541 aagacagttt taaaatggca agcagaacat ccagaatcta agaccttaac tatcattttt
      601 aataccttta catcagaaga caaggcctta tacgacactt atttgcaaaa ggagaataac
      661 tgtgagtaat tggacaacct atcctcagaa aaacctcacc caggcagaac aactggctca
      721 attaatcaaa gaagcagatg cgcttgtagt tggtattgga gctggcatgt ctgcagctga
      781 tggttttact tatatcgggc cgcgttttga gacggcattc ccagatttta ttgctaagta
      841 tcagtttttg gatatgctgc aagctagcct ttttgatttt gaagattggc aagaatattg
      901 ggcttttcaa agtcgctttg tcgctttgaa ttatcttgat cagcccgtag gccagtctta
      961 tcttgattta aaagagattt tggaaaccaa agactatcat attattacta ctaatgctga
     1021 taatgctttt tgggtggctg gttatgatcc tcataacatt tttcacattc aaggagaata
     1081 tggtttgtgg caatgtagcc agcactgcca tcagcaaact tataaggacg atacggtcat
     1141 ccgtcaaatg atcgcagagc aaaaaaacat gaaagtccca ggtcaactga ttcctcattg
     1201 ccctgaatgt gaggcgcctt ttgaaatcaa taagcgtaat gaagaaaaag gaatggttga
     1261 agatgctgac tttcatgccc aaaaagcacg ttatgaggct tttttatcag aacataaaga
     1321 gggtaaagtg ctttatctag aaattggtgt gggacatacg acgcctcagt ttatcaaaca
     1381 ccccttttgg aaacgtgtct cagaaaaccc taatgcctta ttcgttacct tgaatcacaa
     1441 acattaccgc atccctctgt caataaggcg acaaagccta gaactgacag aacacattgc
     1501 acaattgatt agcgccacaa aaaccattta ccaaaaatcg taaccaaagc caatagacgg
     1561 ttaattaaga gatagagatg gagactaaca tgtaccttat tgaaccgatt cgtaacggta
     1621 aacgtatcac cgatggagct gttgctctag ccatgcaagt ttatgttcaa gaaaatctat
     1681 ttttagatga tgatatttta tttccctact attgtgatcc caaagttgaa attggaaaat
     1741 tccagaatgc agttgtagaa actaatcaag agtatttaaa agagcatcat atccctgttg
     1801 ttcgcagaga tactggcggt ggtgccgttt atgtagattc tggtgctgtt aatatttgct
     1861 acttgattaa tgataatggt atttttggtg attttaaaag aacctatcag cccgctattg
     1921 aagcccttca tcatttggga gcaacagagg ttgaaatgtc tggacggaat gacttggtta
     1981 ttgatgggaa aaaggtatct ggcgctgcga tgactattgc taatggccgt gtttatgggg
     2041 gatattccct tttgctagat gtggactttg aagccatgga aaaagcatta aaacctaatc
     2101 gcaaaaaaat tgaatcaaaa ggcatcaggt cagttcgtag tcgcgttgga aacatcagag
     2161 aacatttggc accacaatac caaggtatta cgattgaaga gttcaaagat ttaatggttt
     2221 gccaattgtt acagattgag acgattagcc aagctaagcg ttatgattta acggaaaaag
     2281 actggcaaca aattgatgct ttaacagagc gtaaatatca caattgggaa tggaattatg
     2341 gcaacgcgcc tcaataccgt taccatagag atggccgttt taccggtgga acagtagata
     2401 ttcatttaga tattaaaaaa ggctatattg cagcttgtcg catttacgga gatttttttg
     2461 gcaaggcaga cattgcagaa cttgaaggtc acttgatagg aacacgaatg gaaaaagagg
     2521 atgtcttagc taccctcaat gcaattgatt tagcccctta tttaggtgcg attacagcag
     2581 aagaattagg atatttgata ttttcttgat aaaaaagata cctcagaccg gtaaagtgcg
     2641 attaaaaacg aacaataatt gttttaattc aaataaatgt atgtatgata tgaattgtga
     2701 tataatgacg tcattacgta aaattgagga gtcatcatga aatcagatat cgaaattgcc
     2761 caaagtgtcg ccttacaacc aatcactgac attgttaaaa aggttggaat agacggcgat
     2821 gacattgagc tttatggcaa atataaggct aaattgtctt ttgagaaaat gaaagctgtc
     2881 gaagctaatg agcctggaaa attaatcttg gtaactgcta ttaaccctac tccagcagga
     2941 gaaggaaaat caaccatgtc tattggactg gcagatgcct taaatcaaat gggaaaaaaa
     3001 accatgcttg ccttacgtga accctccctt ggtcctgtta tggggattaa aggcggggca
     3061 gcaggtggtg gttatgccca agtattgcct atggaagaca ttaatcttca ctttactggt
     3121 gacatgcatg ccattacaac cgctaataat gcattatctg ctttgattga caatcatttg
     3181 cagcaaggaa acgatttagg aattgatcct cgccgcatta tttggaagcg cgtgttggac
     3241 cttaatgatc gcgctcttcg tcaggtaatt gttggcttgg gtagtccagt gaatggtgtg
     3301 ccacgcgaag atgggtttga tatcacagta gcttctgaaa tcatggcaat tttatgtttg
     3361 gcaactgact taaaagatct aaagaaacgt cttgctgata tcgtcgtggc ttatacctat
     3421 gatcgtaagc ctgtctatgt gcgtgacttg aaggttgaag gagcattgac attaattcta
     3481 aaagatgcta tcaagccaaa cttggtgcag actatttatg gaacaccagc tcttattcat
     3541 ggggggccat ttgctaatat tgctcatggt tgtaattctg tccttgctac ctcaacagct
     3601 ttgcgattag cagattacac tgttaccgaa gcaggattcg gggccgacct aggagctgaa
     3661 aaattcctaa atatcaaagt acctaatctt cctaaagcac cagatgctat tgtcattgtt
     3721 gcaaccttgc gcgcccttaa aatgcatgga ggtgtcgcca aatcagattt agccgctgaa
     3781 aattgtgagg ctgttcgctt aggatttgcc aacttaaaac gtcacgtgga aaacatgcgc
     3841 caattcaaag ttccagttgt ggtggctatc aatgaatttg tagcagatac cgaggctgaa
     3901 attgctacct taaaagcgct ttgtgaagag attaaggttc cagtagaatt agccagtgtt
     3961 tgggcaaatg gagctgaggg aggcttagca ttagctaaaa cagtggttcg tgtgattgat
     4021 caagaagctg ctgactacaa acgtctctat tcagatgagg atactttgga agaaaaagtg
     4081 atcaatatcg ttacccagat ttatggtggc aaagctgttc aatttggccc caaggccaaa
     4141 acacaattga agcagttcgc agaattcggt tgggataaat tgcctgtttg tatggctaag
     4201 acccaataca gtttttcaga taacccatcc ttgcttggag cgccaaccga ttttgacatt
     4261 accattcgtg aatttgttcc taaaacagga gcaggtttta ttgttggttt aacaggtgat
     4321 gtgatgacca tgcctggttt gcctaaagtt ccagctgcca tggcaatgga cgtagcagaa
     4381 aatggcacag ccctcggtct cttttaggaa aaattgctcg aataattcga gcaagcgctc
     4441 ctttcttccc gctttactgt gatataataa ggtaaatgag attttggagg gatgattatc
     4501 aaaaagaaag caaaagtaaa atatttgctc cataaaggga aacatggatt tttaagagga
     4561 atttttagta gaacaacaat cattgtatta ttaattattt tgcaattagt tttcttattt
     4621 caatcttatg cctggatgga gcagtaccgt gtttggataa cgattcttga aagtgtcttt
     4681 gctattacta ttgttttata tttagttaat agtgatatgg atgctatttc aagaatgacg
     4741 tggttaattt tgattatgat tgctccatta ctagggtcac tgtttttaat ttatacaaaa
     4801 ttggattggg gttacagagg gctaaaacag agaataaatc atcttgtaga tctgtctgca
     4861 ccttacctta gcgatgatga cgctatatta gaggtattaa aggatagcac atcaaccaca
     4921 tatcatttag tacagtactt agaaagaagt cgtggcaact ttccgattta taataataca
     4981 agggtaactt acttcccaac aggtgaaact ttttttgata gtttaaaaga acagttattc
     5041 ttagctaaaa agtacatttt tcttgaattt tttattattg cagaaggcca aatgtgggga
     5101 gaaatcctta gtattctaga aaaaaaagtc agtgaaggtg tagaagttag ggtgttgttt
     5161 gatggcatga acgaactatc aacgctatca tcagattacg ccaagagatt agaacaaata
     5221 gggattaaag ctaaatcatt tttaccgatt tcacccttta tctctaccta ttacaattat
     5281 cgagatcacc gaaaaattgt cgttattgat ggggaggtat cattcactgg aggtattaat
     5341 ctagcagatg agtacattaa tgaagtagag cgttttggcc gctggaaaga tgctggttta
     5401 atgcttgagg gtgaagcaac agacagcttt ttaattttgt ttttacaaat gtggtctatc
     5461 acagaaaaag aactgattat tgatccttat ctttcagatc attctttaaa acttccttca
     5521 gatggctatg ttattcccta cggtgattcc ccgcttgata ctgataaaat aggtaaaaat
     5581 gtttatatag acattttaaa tcatgctaaa gagtacgttt atatcatgac accttacctt
     5641 attttagata gcgagatgga acacgcttta cgatttgcat cagaacgtgg agtagatatc
     5701 cgaattatta tgccaggagt gccagataag ggggtaccat atgccttagc taaaacctat
     5761 tataaagctt tgatgacttc tggagtaaaa atttatgagt atcaaccagg gttcgttcat
     5821 tcaaaggtat ttatctctga taatacaaaa gcagttgtcg gaacaattaa cttagactat
     5881 cgtagccttt atcatcattt tgaatgcgcg acttatttat atcgtgtgtc agttattgct
     5941 gatattgtga atgactttaa tgaagctcaa aagcaatcac tattgatgac atcagatcat
     6001 ttgaagcaac gtccttggta tcaaaaattg ataggattat tagtacgaac aattgcccct
     6061 ctcttgtaat tttcgtaaaa tatgttataa tatctgtatg cgatgagtcg attgtgactg
     6121 caaagtcact tttgcgcagg ggaggtcatt aaggcgggaa gccgactttg ataatctgtg
     6181 tgaaccattt tatcacaatc ttatttatta agaatgcccc ttggattgag atattctcaa
     6241 tccttttttt aattttatta acttagttaa aattcagaat gtatcagtga ttactgtgtt
     6301 tcaaaataag aaagtagttt ttcgggtatg agtgctatga cttgacaaat gctaataaca
     6361 tttgcataat gtaagtagag atgaaaactt atgaaaagag atgatcttat gcaaattatt
     6421 aagcgtgatg gccacgttgc tgattttgat ccagataaaa tttatcaagc aattattaaa
     6481 gcagcaagga cggtctatgt tattgatgac acgtggcgac aaaacttaac acatgtcact
     6541 aaaaaagtag ttattgattt agaagaagca catgtagaac gtcctactat caatatggtc
     6601 caatcccttg tggagaatcg tttgatggat gctggatata ttaccattgc agaacattac
     6661 atttcttatc gtcttcagcg agatttagag cgcaatggct atggtgataa gattgtggtt
     6721 cacttgcgtt ttgaacaaat aaaataaaaa cgcttctaat ggcattttag tgtctttgag
     6781 gggataaggt ttaaatacct aacagataaa aatctgttag gtattttttt gctactatta
     6841 ggtgtattct atattacata gaacttaagc attcaagcct tagtttaact ttctgtttat
     6901 tttttgctac attttgggaa actcccctcc tttcttagtt gaggtgaata aacttttatt
     6961 tgataaatca tatccttagt cttaatgaaa cagtctaaga taatactact tcgaagtgac
     7021 agctagtagt ttccaatttt taagtagtct aaaattatgt ttttttaatt tcaaaaccat
     7081 gctttcgttt gttataaaaa ccgatcttcc atagttagag tttttatcca ggcattgaag
     7141 attggggcaa ctatccaatt agagatttta taatacttat ttatccccat tttttatatt
     7201 aatggtacga agaaaagtta gttgctcttt ccacaagtat ttttgattag cgagtttaat
     7261 ttgctttgta tttgttgatt gatagcaata gagagatagc gtttttttcc agttattatt
     7321 attctatcag atttatctaa tgtaaagttt ctataagagt ctgttgtcgt gtaatcaatc
     7381 actaaatgag taggggttag ttgatagtga atatcagggc aattttgttt taaaaagaga
     7441 gccaattgac gttcccgctg ttttagataa cgcttaataa agcgaagatt tttttgaaac
     7501 atcccatttt ctaaataaag agctaatgct ttttgcatta gtaagttcgt gtctaaatca
     7561 atcaatgatt tttgggttaa aaaatgaggt tttaaacctg acggtaaaac tagagctccg
     7621 atacggagtg caggaaaaac actcattgaa aaagatttta gatagatgat tcggtgatga
     7681 gtgtcataat aatgaattgg tggctcttta agtttcacaa aatctcctaa ataatcatct
     7741 tctaaaatat aaacctggta tcgttgtgct agacgaacaa tagcttcctt ttccttagtg
     7801 ctataagaaa gtcccagagg atgtgaaaat cgtgaaatcg tgtaaaaaaa tttaatatcg
     7861 ccagtttgaa ataaagattc taagagttct aaatcaaggc cattaaagtg tctagagata
     7921 gtttgatagg gtaatcctaa ctgagcaaca atagcttcca tccgatgata agtaggcttt
     7981 tcaaggagaa ttgttttgcc cgcattagga aaaggcattt gtgataaaat ataaagcgct
     8041 tgttgcgtcc cagaggtgat taatagttgg tctttgttac tgtaaacact attttcagct
     8101 aagtaaagaa gcaaagcttc ccttagttct tctagtcctt cggttttgtg ataataatga
     8161 aagaggtatt tgtctttagc acttaaagcc tcatttaagc agagtcggaa atcttcataa
     8221 gccatattat tataatcttc aagacttaga ttaagaacat tatcgggcat gcttacgtta
     8281 cccaaaacat aataaccact tttaggaaca gcataaatta agtgacgata ttttaattct
     8341 aaaagagccc gctgaacagt atctttgcta caatgataaa ccttacttaa taccctaatt
     8401 gaaggtagct tgtccccttt ttttagtcgc tgtttctgga tatcttgttc gatattggaa
     8461 atgattgttt gatattttgt tgtcataaat tgtccccata cagttaagtg ttataactat
     8521 tgtatcttat gccccttttt cttacaatag ttattataga aaagaggtaa tagctatgaa
     8581 acggattgtt gtggcaaatg atctggttgg tgttggtaag gtagccttgt ctgctagtat
     8641 tcctttaatg gcttcttgtt tgttagagca agttttatta ccaacttgcc ttttatcaag
     8701 ccatactggc ggaggacaga gccctgtgaa gcatactaca tcaagtatat tagacgtttt
     8761 tttaacagat tgggacaaca aaagaattaa gtttgacggc atctttatag gttattttac
     8821 gacaacaaga gatgggcaaa agctgctaga atggctttca agaaagcaac ttcccttaat
     8881 tttagatcca attatggcag ataatggaaa actttatcaa ggcttcagtc aaaaacatat
     8941 agagattatg ataaaactta ctcaaaaagc tgagcttgtt ttacctaata ttagtgaggc
     9001 ttgtctatta acacaaacag cttatttagc aaaaaattat gatgaagaag atattagaca
     9061 ggtggctaga aaattagcta agcttggacc taaacaggtt gtcattagcg gaatctcttt
     9121 tgaggaggac agcattggtt ttgcttacta tcagtcagat tcagatcaat taacattcca
     9181 ttttgggaag caattccctt accatttcta tggtacagga gatatagcat cagctattat
     9241 aacagcagga ttctattatc agctaccact aacacaaatt ctagatcttg ttgtttcatt
     9301 tcttgaaaaa gtattgcaaa atacccttac attaaaaaga gatttaatct taggtgttgc
     9361 ttatgaatct catttaaatt acttaactaa atcgtttaaa aagttgttgg aggaaaaaaa
     9421 tgtcatctca aaaattagct aattatattg aattgagtat gtatgccgct ttaattttta
     9481 tttcagtaca agtgttgcgt atccctgtcg gtatgcagtt tattcatctt ggcaatgcat
     9541 tagttgttat tgcagtctta atctatggta gtaaaaaagg agctctagtt gctagtcttg
     9601 gtttagggat ctttgatgtt ttgaacggct atgcttcagt cgtttggata actatcttag
     9661 aatccttggc tatttgttta attttgcatt tggtatatga aagagctatg caatctagta
     9721 aaaaaccaaa agccattata acaataacag ttattgctgc attgtcaaaa ttaactatta
     9781 accttataaa atacttactg ttagcttata taggtactca gctaccactt tcagtatctt
     9841 tttgggtagc tcttggaaaa attggtggta gtttcggaac atcttttgtg actttagttg
     9901 ctgttcctct tttgtatcct gttatcaaaa acttaaaaga aaaggttaca atcggacaag
     9961 caaaaagagg tttttgatac aagtacctta tcgataaaaa agcaaggtac acttttagtc
    10021 aaatattgac aaaaggttac aattttgcca taatgaaata actataaagc cgttttgacg
    10081 gcttttttga tgacagttaa ggaagaaacg atgagtattt tagaagttaa gcagttgagt
    10141 cacggttttg gggatcgggc tatttttgaa aatgtgtcat ttcgcctctt aaaaggcgaa
    10201 catattggac tagttggggc aaatggtgaa ggaaaatcaa cctttatgag tatagtcaca
    10261 ggacatttac agcctgatga aggaaaagta gagtggtcga agtatgtcac tgcaggttac
    10321 ctggatcaac atacagtgtt ggaatcagga caaaccgttc gtgatgtctt gcgaactgct
    10381 tttgatgagt tatttaagac cgagaatcgt attaatgaga tttacgcgtc aatggcagat
    10441 gataaagctg atattgctgt tttgatggaa gaagtaggtg agcttcaaga tcgtttagaa
    10501 agtcgtgatt tctatacttt ggatgctaag attgatgaag tagcgcgtgc gcttggtgtt
    10561 atggattttg gaatggagtc agatgttaca tccttatcag gtgggcaacg aacaaaggtt
    10621 ttattagcca aattactatt agaaaaacct gatattctgc tattagatga accaactaac
    10681 catttggatg ctgagcatat tgaatggtta aaacgctatt tacagcatta tgaaaatgct
    10741 tttgtgttga tttcgcatga tatttctttc ttaaatgatg tgattaatat tgtttaccat
    10801 gttgaaaatc aaagtttagt tcgctatact ggggattatt accaatttca agctgtttat
    10861 gagatgaaac aatctcaact tgaagcagcc tatgaacgtc aacaaaaaga gattgctaac
    10921 ttgcaggatt ttgtcaaccg aaataaggct cgggtagcga cacgtaacat ggcaatgtct
    10981 cgccaaaaaa aacttgataa gatggatata attgaacttc aagctgagaa accaaaacca
    11041 aattttgaat ttaagcaagc tagaactccc agtcgattca tttttcaaac aaaaaatctt
    11101 gtgattggtt atgattaccc attgaccaaa gaacccttaa atataacgtt tgaaagaaac
    11161 caaaaaattg ctattgttgg ggccaacggt attggaaaat ctactttgct aaaaagttta
    11221 ttaggtgtta tcgagccttt agaaggtcat attgtcacag gggatttttt agaagttggc
    11281 tactttgaac aagaagtgac aggtattaac cgacaaactc cgctagaagt agtttgggat
    11341 gcttttcctg ccttaaatca ggcagaagtt cgagcggcac tagctcgttg cggactaaca
    11401 tcaaaacata tcgaaagtca aattcaagta ctttcgggtg gtgaacaagc aaaagttcgt
    11461 ttttgtttgt tgatgaatcg tgaaaataac gtgcttattt tagacgaacc aacaaatcat
    11521 cttgatattg atgctaaaaa tgagctcaaa cgtgctttaa aagcatataa ggcttctatt
    11581 ttaatggttt gtcatgaacc tgatttctac aatgggtggg taaccgatac ttgggatttt
    11641 agtaagttaa cctaaactat tccttaattt tttacacaga aattactaaa atcagtaaag
    11701 atatagaata aatactgcta gatatgtgat ggtttaggta actgatcacc aaccaaaaaa
    11761 gatgtgacta catctttttt ttatctaatg gaatggtgta aagtcgttta ataatagctt
    11821 attttttgat gatcagttgt tcatgaaatt gttggtgatt aacaagtcat ttaagataca
    11881 aacgttatta ctaaaaagtg attcaaatag tttccctatt tttatgtttt tttaaaattt
    11941 attacgaata aatagataaa gacagtgaag atgattagta agtacatctg ctaagattac
    12001 gaatagatgg ggacaataaa tgacgaattt aacgttttat gcaaaaatcg gtatttcaga
    12061 agaagagcat gactcttttg ttaaagaaca tcagcaaatt agcgttttac aaggtagtga
    12121 ttgggcaaaa atcaaaaatc aatggcagaa tgagcgaatt ggtatctata aagaggaaaa
    12181 gcaggttgcc tctttatcac ttttgattaa gctattgcca cttggaagaa gcattattta
    12241 tattccaaga gggccagtca tggattatct tgaccgtgat ttggtggcat ttaccatgaa
    12301 aacactaaag gattatggta aaactaaaaa ggccctcttt atcaaatatg atccagctat
    12361 cctgttaaaa caatacgcac tgggacagga agaagaagaa aaacctttag ctttagcagc
    12421 tattaagaat ctccaagaag ctggtgttca ttggactggt ttaacaatgg agattgcaga
    12481 tagtatccaa cctcgtttcc aagctaatat ttacactcaa gaaaaccttg agatgcaatt
    12541 tcctaagcat accagacgtt taataaaaga tgctaagcag cgtggtgtag aaacatatcg
    12601 tgccagtcaa tcagaacttc acaaattttc caagattgtc tccttaacag aaaagcgtaa
    12661 aaatatttct ttgcgtaacg aagcttattt tcaaaagttg atgactactt atggggatag
    12721 ggcctactta catctagcaa aagtgaatat tcctcaaaaa ctagatcaat accgccagca
    12781 attaaatctt attaaccaag atattactcg cacccaagct catcaaaaga agcgtttaaa
    12841 aaaattagaa gatcaaaaag cttctttaga acgttatata actgaatttg aaggctttac
    12901 agaccaatat cctgaggaag ttgttgtagc aggtatatta tctatttctt atggaaatgt
    12961 tatggaaatg ctttatgctg ggatgaatga tgattttaag aagttttatc ctcagtatct
    13021 gctgtatcct aaagtttttc aggatgctta tcaagatggt attatttggg ctaacatggg
    13081 aggagtagaa ggctcgcttg atgatggact taccaaattt aaggccaact ttgctccgac
    13141 aatagaagaa tttataggag aatttaatct ccctgtcagc ccactttatc atattgctaa
    13201 taccatgtac aaaatacgaa aacagttaaa gaataaacat taaaagactc gccaaaatga
    13261 gcgagtcttt taatgtttat tctgttattc ccattcaact gttgcgggtg gtttacttgt
    13321 gatgtcgtag acgatacggt taacgtggtc aacttcattt acgatacgtg ttgagatttt
    13381 tttcaagaca tcccaaggaa gttgagcaaa gtcagctgtc ataccatcaa tagacgtaat
    13441 agcacgaatg gcgatggtat aatcataagt acgaccatct cccatgacgc ctacagaacg
    13501 gacacctgtg ttaactgtaa agtattgcca cacgtcacga tcaagtccag ccttagcaat
    13561 ttcttcacga aggatagcgt ctgattcgcg aacggtttca agtttttctt cagtaattgc
    13621 tcccatgaca cggatagcaa gtccaggacc tggaaatggt tggcgccaaa caatttcttc
    13681 aggcattcca agagcgattc caagcgctcg aacttcatct ttgaaaagag tgtttaaggg
    13741 ctcaatcaat tcaaactgca tgtcttcggg gagaccaccc acattgtgat gtgatttgat
    13801 ggtttgagca gtttctgttc ctgactcaat gatatcagta taaagtgttc cttgggcaag
    13861 gaagtcaaca ccttttaatt tgctggcttc atcatcaaaa acatagacaa attcattacc
    13921 aataatttta cgttttttct caggatcttc aacgtctgca agaaggtcta agaaacgttt
    13981 tgaagcatcc acacggataa tatttaggcc aaatttgccc ccaagcattc ccataacttg
    14041 atcgccctcg tctttacgaa gaagaccgtg atcaacgaaa atacaagtta attggtcacc
    14101 gatagctttt tgaagtagaa caccaacaac tgaagaatca actccaccag aaagacctag
    14161 aagaacttta cgatcgccta cagtttcacg aattttagta atttccatgt caataaaatt
    14221 atccattgac caatcaccac gcgcgccaca aattgatata gcaaagtttt taagaatgtc
    14281 attcccataa acagagtgtc tcacttctgg gtggaactga ataccgtaaa ggtttttctc
    14341 agtattttca atagctgcat agggacagtc atttgagtct ccaacaaggt ggaaaccttc
    14401 tggaatttca gtaacagcat caccatggct catcaaaacg agttgttctt gaggtgtgcc
    14461 tgaaaataat tttgacgttt cacgaagatg aagggttgac tgaccgtatt cacgattacc
    14521 agcttgtcca gcaggaacaa ctttaccacc taatttatgg gtgattaatt gcataccgta
    14581 acagatacca agaatcggaa tccctagttc aaagatttca gggtcaatgc caaaggcgtt
    14641 atcagcgtaa acagagttag gccctcctga taaaacgata cctatgggat tgatctcatg
    14701 aagttcttga gcggtgattt tatggctttt tagttcggag aaaacaccaa actctcgaat
    14761 acgtctagca ataagctgat tgtactggct accataatca agaacgataa ttttttgaac
    14821 atcattcaaa attgaaattt cagtcattat aatcctttca ttctatacca accgtcaggc
    14881 tgatagttgt caacttattg tagcatattt tcatcgaaaa gacattacaa tttctctagc
    14941 tcgttggaaa aaaacgtaat cgttatgtta caatagaggc tatgaaaggg aattaagaca
    15001 acagggaggg cctatgctaa gaaaaaagag aattttgggt ttatttcgac ctgttgaatt
    15061 aattttccta ggattattgt tgagtttagt tgtttcgtac cttgcgtgga ccaatagttt
    15121 tgcaacactt cataatatat tagcaactgt cggaatagtg gaacgaagca aagatcaaca
    15181 gcctcgctat cacattggac aagctattca agtacaaaaa agtggtccat atcatcaatg
    15241 gattggaact attaataaac aggtagaaga tatagccgaa aattaccgag tgagttatca
    15301 ttatgaagtg gtatttccaa taggaaaagt cactgtttct ttgccagaac ataacctgaa
    15361 aaagcctgat aaaccgaggt ttaaaaaagg agatatcgtt aaattatctt cattaactaa
    15421 aaagccacat ataaaagtgt atcaaggtca attagcgact attaaacaag ttaaaaaacg
    15481 ctatgactat tcgttaggag gatatcagta cgatattaac ctgaaagata atctaagatt
    15541 agatggaatt tcagagcaag attttgttaa accttattat attaggttca ataaaggaaa
    15601 ttcccctgag caaaacaatc gtcttttgcg aaaagctttc gcctatgcaa agcagcatcc
    15661 aaatagcgtt atatcttttc caaaggggca atttcacatt ggctctttgc cttcacaaaa
    15721 agattatttt gagcttccat ctgatacagc tattattggt catcagacag agttcattat
    15781 tcacggtaaa atgttgtggt ttggattccc tacaggacca aaggctgaac aaggtgttcg
    15841 taatctcgtg ttgactggag tgcatttcaa agcaaatgat ttgaaaaaag gagaccactt
    15901 tatgattatg gctgatcatg gtactgattg gcatatttac gataacaaat ttactatggt
    15961 tcataagcgt aatagtcata tttttgattt aggatctcta caaaattcat tgtttgagaa
    16021 aaaccaattt attggctatg cgccagaatt agtacaagac caacagctgc tatcaaaggc
    16081 tcaagggcat gatttttttt cagaagtcat tcagtttgat gctgctgttc atcattttgc
    16141 atgggatgga ggtctactta gtaatattgc tccaaactat gaagcattta accaaactcg
    16201 acatctatgt cacaatatta ctgtaagcca aaatcaattt ttaccttata tagatccgac
    16261 tggttgcctg agagcctata gtggttctat tggtcagcat tcctcaaaag taggagttat
    16321 tagggtttta aataatgttt ttacctcatc cattgttact aaagcgaagc tcactagttg
    16381 gtttatggaa cctattcatt ttccaccaaa ttcaccagtt attgtcgcag gtaatatcat
    16441 taattgatgc caaatacctc aatatttaca attcaatttt catcaaaaat atgataaaat
    16501 agatgaataa ccgacttgag gagtgtggca tgttaccagc ttatataaaa attcatgatg
    16561 caattaaaaa agacattgat ctgggcatct ggccaattgg tagtcgctta ccaagtgaac
    16621 gacatttagc ggaacatttc accgttagtc gaatgactct acggcaggct attacattac
    16681 tagtagaaga agggattctt gaaaggcgga taggaagtgg tacttatgtt gccagtcatc
    16741 gtgttcaaga aaaaatgcga ggaacaacaa gtttcacaga aattatacgc tcccaaggac
    16801 gacaaccctc ctctaaatta ttatcctatc aaaagcaatt agctagtgat accgaagtta
    16861 aagaattgaa cttagacaaa acagacttgg tcattcgaat ggaacgtatc cgctatgcag
    16921 acagtgtccc tttggtgtac gaaattgctt caatcccaga aaaatttata aaaacggtta
    16981 aacgagctga tattacagaa cacttttttc actcattgat agcaaatggt tacgagattg
    17041 gtaaaagtaa acagacaatc tacgctaagc tagcaagtga gcgtgtagcc tcctacttgg
    17101 aagttgctaa aggacatgcc attttggctc taactcaggt gtcttacttt acagacggca
    17161 aaccttttga gtatgtccgc agtcagtata tcggtgaccg ttttgaattt tacttagaaa
    17221 ataattagta aagaagtcat ttatgacttc cttttttaca cgaacagcat aaggcaatta
    17281 atttgctact gttaaaaaca atcatagtat gataaaatga acattatgga aattgaaaaa
    17341 acaaacagga tgaatgccct ttttgaattt tatgcagctt tgctaacaga taaacaaatg
    17401 aattatattg agctgtatta tgccgatgat tatagcttag ctgagattgc tgatgaattt
    17461 ggtgtcagtc gtcaggctgt ctatgataat attaagcgta cggaaaaaat tttagagact
    17521 tatgagatga aacttcatat gtattcggat tatgttgttc gaagtgaaat ctttgacgac
    17581 atgattgctc actaccctca tgatgagtat ttgcaagaaa aaatttccat tctaaccagt
    17641 attgacaata gagaataaga ggagttttga ctatggcttt tgaaagctta acccaacgtt
    17701 tgcaagatgt gtttaaacac attcgcggta aaaagaaatt atcagaatca gatgttcaag
    17761 aagtgacaaa agagattcgt ctagctcttt tagaagccga tgtcgcttta ccagttgtta
    17821 aaacctttat taagcgcgta cgtgagcgcg ctattggcca tgagattatt gatactcttg
    17881 atccaacaca acaaattctt aagattgtta atgaagaatt aacgagtatt ttaggctcag
    17941 aaaccgcgga gattgataaa tctccaaaaa ttccaactat tatcatgatg gtcggtttgc
    18001 aaggggctgg taaaaccact tttgcaggaa aactcgctaa taaattaatt aaagaagaaa
    18061 acgcgcgacc actgatgatt gctgccgata tttaccgtcc agcggccatt gatcagttaa
    18121 aaacactggg gcaacaaatc aatgtacctg tttttgatat ggggacagac cactcagcag
    18181 tcgatattgt cagaaaaggt ttggaacaag ctcgtgaaaa ccgcaatgac tatgtcttga
    18241 ttgatactgc gggtcgcttg caaattgatg aaaaactgat ggaagagctg cgcgatgtca
    18301 aggctttagc acaaccaaat gaaattcttt tggttgtaga tagcatgatt ggtcaagaag
    18361 ctgccaatgt agcctacgag tttaatcatc agttgagcat cacaggggtt gtgttaacaa
    18421 agattgatgg tgatacccgt ggaggtgcag cactatctgt ccgtgaaatc actggaaaac
    18481 caattaaatt tacaggtatt ggtgaaaaaa tcacagatat tgaaacattt cacccagacc
    18541 gcatgtccag tcggattttg ggaatgggtg acttactcac tcttattgaa aaagctagcc
    18601 aggaatatga tgagaaaaaa tccttagagt tagctgaaaa aatgcgtgag aacacctttg
    18661 atttcaatga tttcattgaa cagttagatc aagtgcaaaa tatggggcct atggaagacc
    18721 tcttgaaaat gattccagga atggctggta acccagcttt ggccaatatc aaagttgacg
    18781 aaaaccaaat tgctcgcaaa cgtgctattg tctcttcgat gacccccgct gaacgtgaaa
    18841 accctgactt actaaacccg agtcgtcgcc gtcgtattgc agctggttcc gggaacagct
    18901 ttgtggaagt taataaattt atcaaagact ttaaccaagc caaaagcatg atgcaagggg
    18961 ttatgtcggg cgatatgtct aagatgatga aagatatggg cattaatccc aataatcttc
    19021 ctaaaaacat gcctgctggt atgccagata tgtcttcctt agaagggatg atgggacaag
    19081 gtggtatgcc tgatttatct ggccttggtg gtgatatgga tatgagtcaa ctatttggta
    19141 aagggttcaa ggggaaaatt ggacaatttg ccatgaaaca ggccatgaaa cgtcaagcta
    19201 acaaactaaa aaaagcaaag aaaaaacgaa aataaaagag acggaaattt tcgtctcttt
    19261 ttgatgtgaa attcatttat cttgtataca agattattaa aattatggaa gaccgcgtta
    19321 catgcatgat gagttcattt aaaatttgaa ataacctaat caatctaact taaatttcta
    19381 agggcctaaa attagcgata attttgccaa taatgcgagg ttcttcgcta tagggagcaa
    19441 acttatcaga atatttttta tttaaggaca ctagacgtaa tccctcatct tcacgaaata
    19501 cctttttgat atatgtttgc ccatcccatt cgactgcata aattgctcca tcgtaatcaa
    19561 aactattttg ttttataaga acaacttcgc catttaaata agttggctcc atagaatctc
    19621 caaaaaccca agacgcaaaa tcgtattcta attgttcatc gtaaaagaca acatcaaagt
    19681 taccatcacc aaaataggag taaccagtac cagcagataa actttcatag acccgataag
    19741 catataattg tgatggttta tctatgagat ctttagattt tttgtctatt tgatgattta
    19801 acaatcgctc tgaatagcca attacttttt ctttattaga tattgtcagc tggttataag
    19861 gcgttagcaa tctatagttt gggtcgaaat aatcagatgt cacattgaat agatgcaata
    19921 aggcatttaa atgtttttca tttggtatat tcttaccttt ttcccaatta gatattgtca
    19981 ttttattgac atgcaacaat cttcccaatt ctgattgagt tattcccctt gataatcgtg
    20041 ctgtttttag ttgatgtcca gaaaacattt ggtagctcct ttagtaaagt tataacttta
    20101 ctaatagtat caaaaaaata aaacgaaagc aagtgtaaca tttcctacaa ttaattcatg
    20161 tgatagcaat actgtcaaaa aggggaaaca cctaaaacat agcgactaga tttgtgtact
    20221 agcaggtcta tatctgataa agagttaatt actttaaaga taaaattatc atttagttct
    20281 gagctaaaca aattctgtgc ctgaagttac taaaataatg atagtaaatt attactgaat
    20341 ctaaaagaag tgacgttagt atctgtaaaa aaagagattt gtctcgtgac atctttccga
    20401 aaaactgtaa ttttcttgaa aaatatttat aggtgtgata tactgctctt gtaaagaatg
    20461 aatggcataa aatacatgag acaatttgcg aaaattcatt tttgaaaaaa ttgttattgt
    20521 taaggaatta tgatgatatg agacgcgaat tattactaga aaaaattgaa acctataagg
    20581 ccatcatgcc ttggtatgtt ttagattatt accaatccaa gttagctgtt ccatacagct
    20641 ttaccacctt atatgagtat ttaaaagaat acaaacgctt ttttgattgg ctgatggatg
    20701 ctgatttaac gcaggcgcca aagattgctg atattgactt gagcacgctt gagcacctta
    20761 ccaagaagga tttagaggcc tttgtgcttt atttgcggga acgaccttct ctcaacacct
    20821 attccaccaa agagggtctt tctcaaacca ctattaatcg gaccttgtct gccttatcaa
    20881 gcctttataa gtacttgact gaagaggttg aaaatgacca aggagagcct tatttttatc
    20941 ggaatgttat gaaaaaagtg tcaacaaaaa agaaaaaaga aaccctggct tccagagcgg
    21001 aaaatattaa gcagaaactg tttttaggtg atgaaactct agctttcttg gattatgtgg
    21061 ataaggaata tgagcaaaaa ttgtctaatc gtgccaaatc ttctttccgt aaaaataagg
    21121 agagggattt ggctattatt tctctactac ttgcttctgg tgttcgtttg tctgaagcag
    21181 tgaatcttga tttaaaagat gtcaatctaa atatgatgat tatcgaagtt attcgcaaag
    21241 gcggaaaacg tgattcagtc aatgtagcag gttttgcgaa aggttatttg gagtcttatt
    21301 tagctgttcg tcagagacgt tacaaggctg aaaaacagga tcttgctttc tttttaacag
    21361 aataccgtgg cgttccaaat cgaatggatg cttctagtat agaaaagatg gttggcaagt
    21421 actctgagga ttttaaaatt cgtgtgactc ctcacaaatt gcgtcatacg ctagcgacaa
    21481 gactatatga tgctactaaa tctcaggtgt tagttagtca tcaattaggc cattcctcta
    21541 ctcaagtcac ggatctttat acccatattg tcaatgatga gcaaaaaaat gctttagata
    21601 acctttaatg ttacataatt gtttttatgt aacattaaag gtaaaaatag cttcaaaatt
    21661 aactttaata gatggctact attaaccgtg acagtttggt gtcagaccgt tttccaaact
    21721 actcaagcca cttagaaaaa ggaatatcac tgctaacttt gtcccttctt tgaatgactt
    21781 tttttgcgat gtttatagat taaatctagt agggttctca tcaaaaagat gagataagcc
    21841 tatttttagt atgtcagtac ttttatttag tatgataata tgttatcttg taaagtagct
    21901 tttaggatta ttcttgttaa aatacttaat gttattactg ataacttaga gtaatataaa
    21961 gctaaaattg aaggaatatt agagaatcat gaagacagct tctttaacat caattggttg
    22021 aatacaagga tcagataaca ttatcgctag atagtctgaa gaatcaaaaa caaagtttgt
    22081 tacataaata aattcttgtc aaaacttgta ttttctattt gaactgatac gattgacaaa
    22141 ggctaaacta aaatagcttg acttttttag aatctttgta ttttttgtcg gatcccctcc
    22201 tattttttcc ttgaattgat ctatttaatc ttttagaaca aggtttatcg catactattt
    22261 ttagacaatt tgttataata aaaagaaacg acttgaggag ataaaagatg ccaaagagaa
    22321 taagaaaagc tctaggagta gttgggaaac tgatgtcaat tgttcctgac actactgaaa
    22381 ttattggaaa aacgattgat aacagtcgac caattatcga aaaacgtatg gaacaaaaac
    22441 atgaaaaaga gatgcaacta cgcacgatag atgatgtgat taatcttact gttgatcagg
    22501 cacaagctca tttggaacaa cttggctttg tagtagccac tatacctgct aagcctcata
    22561 aaaaatggct tcatagtaat ctcaatgaag tggttgctat gtctccaaaa tctggtaaat
    22621 ataagatagg aagcctgatc aaactctact acattactgt tgatgttttg gaaaaaagtc
    22681 aagacttatt agaccaagaa aacttgcgga ctgtggaacg caatcaaaaa atcgctgata
    22741 cttttgagtc tgtcaaacac attcgatttc cttttaaaaa atggcgttga taaaaaacag
    22801 agttgccttt agatagatca gcctgttttg gtactaagtt atgttatgat tcaataaaat
    22861 taaagataag gagaagaaat gccttactac actatttcaa aagtctttcc ttcagataaa
    22921 acgacaatgg ctagtgtaaa gaacctgctt catcaagaag gtattcgttt ggatactcat
    22981 ttagattata cttgtgccat catgaatgcg caaaatgatg tgattgctac cggttcttac
    23041 tttggaaata gcttgcgctg tctttgcgta tccagtgcct atcaaggtga aggacttctt
    23101 aataggattg tgagtcatct cattgatgaa gaatatgccc ttggcaacta tcatcttttt
    23161 gtttatacga agacctcttc tgctgctttt ttcaaagatc ttggttttac tgaaattgtc
    23221 catatcgaca atcacatcag tttcttggaa aataaaaaaa ctggttttca agattatctg
    23281 atgacactta ataagcctga gcaaacacct ggtaaagtcg ctgctattgt tatcaacgct
    23341 aaccccttta ccttagggca tcaattttta gtagagaaag ctgcaagaga aaatgattgg
    23401 gttcatctgt ttatggtcag tgaagaccgc agtctaattc ctttttcggt gagaaagagg
    23461 ttgattcaag aaggtctagc tcatcttgat aatgtcatat atcatgaaac aggtccttat
    23521 ttgattagcc aagcgacttt cccagcctat ttccaaaaag aagacaatga cgtgattaag
    23581 agccaagctt tgctggatac cgctattttt ctaaagattg ctcaaacctt acagattaca
    23641 aaaagatacg ttggagaaga gccaaccagc cgagtaactg ctatttacaa tgaaattatg
    23701 gcagagcagt tgcagcaagc tggtatcctc ctagatattt tgccaaggaa agctatcaat
    23761 cagcagcaag atcctatcag tgcctcaacg gctaggcagg cattgaaaga taatgattgg
    23821 gaacttctgg caaaactcct tcccaaaacg tctttagatt atttttgttc gctagaagcc
    23881 caacccatta ttaaaaagat acaagccact tcatctgtca aacattacta agataaaaaa
    23941 cctatctgat tatcaaaatc aggtaggttt ttggtattgt tcttacagca aatgcgtttc
    24001 accaaggaga ttaatggtta agaggattag tcattccctg ttagtcttga atgtccagtt
    24061 gtacagtaac ttcttgaacc ggaacatcat agaaaggatc ttcacggcgt cccaagaaat
    24121 ctctggcttg ctgtgggaaa ctcgcatagc ttaacacgtc ttcttctgaa cgagcgtatg
    24181 gagcaatctc gtcacgtaga taaatcattt gaggctcgat taggtcggct ggtctgcaag
    24241 taataactac ttcgtcacca atgattttct ctttgatacc ttctgctaat ggcgctggtg
    24301 attgaccgta tagccctcga acatagtctt tgatttcatt tggaactacc ttgtagcgtt
    24361 cccctgagat gatattcatc aaggcctgtg tgccaaccat ttgtgataat ggcgttacaa
    24421 gtggtggata tccaagatcg gcccttactt taggtacttc tgctaagact tcttcgtatt
    24481 tatcagctag gccttgctcg gttaattggc tcaataggtt tgatagcatg ccacctggaa
    24541 cttggtaaat caatgttttt ggctcagtat ctttgacttt aggatttaaa atccctgtct
    24601 ctcgatagtg gtcacgaata gtgttgaaat attctgcaac tttggccacc tcttgcatat
    24661 ccaagccagt gtcaaagcct aaatccgtca aggcaatcgc cattgactct gtggcaggct
    24721 gactggttcc ccctgaaaaa gaggaaatcg ccgtatcaat aatatcagct cctgcttctg
    24781 ctacttttaa gtaagtcatt tctgaaatac cactggtagc atgggtatga acctcaagag
    24841 ggatagttgt attctccttg atacatttga ccaattggta acctatttca ggagttaaga
    24901 caccagccat gtctttgata caaatggagt ccgctccgat agcttggtaa gctttcgcca
    24961 attcaacaaa gtaatcaatg gtatataccg gacttgtcgt gtaagaaatg gcaacttgag
    25021 catgccctcc aaatttttta gtcgctgaaa cagctgtttg caagttgcga gggtcattta
    25081 aagcatcgaa gatacggaca atatcaatcc cgttttcaat ggatttttga acgaaggatc
    25141 tgaccacatc gtcagcataa ttgcgatatc caagaaggtt ttgtccgcgt aagagcatct
    25201 gaagtttagt ctttttcact gctttacgga tggctcttag ccgttcccaa gggtcttcat
    25261 ttaaaaagcg taggcaggaa tcaaaggttg ctcctcccca catttcaagg gcatgataac
    25321 cagcattgtc tagcgtttcg agaataggaa tcatttcctt ggttgtcata cgtgtggcaa
    25381 tctgactttg ttgaccatca cgtaaaactg tttccgttat acggattttc gtcataattg
    25441 ttctcctcat caacctgtct tatctgatga cgatgattgg ttgtctttat tgaaaaatga
    25501 atggagtaat tttgaaatag cagtctgcat ctcctcgaca ctgtgcttac gtaggcgacc
    25561 gcagactttg gcctctttag aacagataaa acattgccta ggggagcctc ctaatacggt
    25621 tctgctaatt gaatgaggca gatcattttg caagaccaag acatctaagt ccatgagacg
    25681 acctattggc aactctgtct ctaaggctat catttttaac ttcaggtcgc ggctaggtag
    25741 acttgtgatg aggtaatact cataacccgt tgtaggcagt aatcgcaact ggtaacaaat
    25801 tttatcgtca gctaaggcag tttgaatggc cttaatcact atatcaaaag cttcaagaag
    25861 tttaggagag gttttaattg gtccagggat attcatggtg accgataata agctaccttc
    25921 tgggtattcc tttaataaat gcagttgacg cagagctctt tcttcgcggg ctcttaacat
    25981 atcactaagt tggatagctt cgcctgaaaa ataagtgtct ttacacattg taaaccacat
    26041 ccatgaggct accatcacgg tattcaataa gagcaacaac cttatcacca tattgaatac
    26101 gctcaggtgt gccaacgatg gcgtaagcct tctcttttag ctcctcaata ctaaattgtg
    26161 gcacattgag ggatttgaaa tggtctacta agtcttgacg atttgggtta atggcaattc
    26221 ccacttctgt gacgaccaca tcgacacttg ttcctggtgt aatgaccgta ttaacctcat
    26281 ctacaaaagt tgggatgcgt ccgcggataa gtggtgaaat aaccaaactc atcttagccg
    26341 caaaggcagt gtcgcagtgt cctccagaag cgccacgaat cacgccgtca gatcctgtca
    26401 tcacattaac attgaagtta gtatcaactt caagggctga taaaatacaa gtgtctaatt
    26461 gattgataac agcacccttg ctcaaaggtg aggcatacat gttggcatca atttcataat
    26521 gttcagcatg cttaccaaga gaaacagctg atgggtgatc aaagtcttgg acatcaagaa
    26581 ttttttcaac cagtccttct tcgagcaact ccaccatagc attggtaata ccaccaagag
    26641 caaagctagc tttgatattt tctttaatca tagcttc
//
LOCUS       DAUCLR010000020        26433 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.020, whole genome shotgun sequence.
ACCESSION   DAUCLR010000020 DAUCLR010000000
VERSION     DAUCLR010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26433
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.020"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            64..1419
                     /gene="nga"
                     /locus_tag="VGT87_000655"
     CDS             64..1419
                     /gene="nga"
                     /locus_tag="VGT87_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotine adenine dinucleotide glycohydrolase"
                     /protein_id="HEQ3813533.1"
                     /translation="MRNKKVTLAHIVAKTSVAIALAGAMGSSLLANSTTYAVSGKENK
                     KSDVKYETTKVMEANATSSKEDNHVMHTLDGSMSTVWEENSPGGGVGEVLSYKFASPM
                     RIGRILIVNGDTSSKENYYKKNRIAKADVKYYNGNKLVLFQKIELGDTYTKKPHHIEI
                     DKKLDVDRIDIEVTEVHQGQNKDILALSEVTFGNMERDLFEKKFKEIKDKWVTDKQAD
                     EFIETADKYADKAVQMSAVASRAEYYRMYVSRKYQYKKEFVEKLKQVYKESGASHVTS
                     KKDLMLAFDDAKRKSTIGRQENGLFVTSFAEDMALLFTDQGKLKSADQIENIKGVDSG
                     RYSDGVYQYEYDSELTKNIDKLGYIRTASGDTPGANSLNIPGCQTWSGKHIENSESEL
                     IFPSISVKDLKSKAVLAEIDAKGYFEIIDPTIIAPNGDHKKVTGRFKIKKMQDRMQDR
                     K"
     gene            1424..1909
                     /locus_tag="VGT87_000656"
     CDS             1424..1909
                     /locus_tag="VGT87_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD glycohydrolase inhibitor"
                     /protein_id="HEQ3813534.1"
                     /translation="MYKVPKGLEHYQKMFQKEVTVNDFKKYLIGSDKEYRITRRDSYM
                     GDISDPEVILEYGVYPAFIKGYTQLKANIEEALLEMSNSGQALDIYQAVQTLNAENML
                     LNYYESLPFYLNRQSILANITKALKDAHIREAMAHYKLGEFAHYQDTMLDMVERTIET
                     F"
     gene            1933..3648
                     /gene="slo"
                     /locus_tag="VGT87_000657"
     CDS             1933..3648
                     /gene="slo"
                     /locus_tag="VGT87_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_095889392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin streptolysin O"
                     /protein_id="HEQ3813535.1"
                     /translation="MSNKKTFKKYSRVAGLLTAALIIGNLVTANAESNKQNTASTETT
                     TTNEQPKAESSELTIEKADQKMDDMLNSNDMIKLAPKEMPLESAEKEEKKSEDKKKSE
                     EDHTEEINDKIYSLNYNELEVLAKNGETIENFVPKEGVKKADKFIVIERKKKNINTTP
                     VDISIIDSVTDMTYPAALQLADKGFTENKPDAVVTKRNPQKIHIDLPGMGDKATVEVN
                     DPTYANVSTAIDNLVNQWHDNYSGGNTLPARTQYTESMVYSKSQIEAALNVNSKILDG
                     TLGIDFKSISKGEKKVMIAAYKQIFYTVSANLPNNPADVFDKSVTFKDLQRKGVSNEA
                     PPLFVSNVAYGRTVFVKLETSSKSNDVEAAFSAALKGTDVKTNGKYSDILENSSFTAV
                     VLGGDAAEHNKVVTKDFDVIRNVIKDNATFSRKNPAYPISYTSVFLKNNKIAGVNNRT
                     EYVETTSTEYTSGKINLSHQGAYVAQYEILWDEINYDDKGKEVITKRRWDNNWYSKTS
                     PFSTVIPLGANSRNIRIMARECTGLAWEWWRKVIDERDVKLSKEINVNISGSTLSPYG
                     SITYK"
     gene            3902..4288
                     /locus_tag="VGT87_000658"
     CDS             3902..4288
                     /locus_tag="VGT87_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813536.1"
                     /translation="MKQQSYQPLRFVYLLVALFAAMLLVARPVMADEEQTPEDRAYSQ
                     GLEKGTEAGRKAGEEATWTDLTPTVPTDLETPDDIEDTNKQLYKEGYTDGYKEGYNEG
                     FGEHYPLLIPVQFIWGIILHWWEMLS"
     gene            complement(4569..4799)
                     /locus_tag="VGT87_000659"
     CDS             complement(4569..4799)
                     /locus_tag="VGT87_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813537.1"
                     /translation="MKKKLSLFMIATAAVVGLSLATPENQTVSANEAVATQACNYDFY
                     GYCGPISWLFYKTKHLLMGEQPTTTSTATVTK"
     gene            complement(5211..5378)
                     /locus_tag="VGT87_000660"
     CDS             complement(5211..5378)
                     /locus_tag="VGT87_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813538.1"
                     /translation="MKKKLSLFMIATAAVAGLSLATLGEHQTVSANDANYSNGFYGHN
                     GPESWLLYTVW"
     gene            complement(5563..5844)
                     /locus_tag="VGT87_000661"
     CDS             complement(5563..5844)
                     /locus_tag="VGT87_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813539.1"
                     /translation="MKNKLFLVALATVTVLGPSLATPHHQTVHAAEQALAGTCTYSNE
                     NPYVGVCFERKDGMPIEENIITKNNDFSLLNWFWEIVTNWWSKITGYFS"
     gene            6329..7468
                     /locus_tag="VGT87_000662"
     CDS             6329..7468
                     /locus_tag="VGT87_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEQ3813540.1"
                     /translation="MRENTTLLHGYTVIDEFTGAASVPIYQTSTFHNSELYCPSQKHL
                     YTRFSNPTTEALEDGLACLEKATYAVAYASGMAAISTVLMLLKAGDHVIFPLEVYGGT
                     CQFATAILPNYQIETSFVDMADLATVKASIRPNTRMIYLETPSNPLLKICDISELVQL
                     AKAYGVLTVADNTFMTSLYQEPLAMGVDIVVESVTKFINGHSDVVAGLAATNNEAIYN
                     QLKLFQKNFGAIVGVEDAWLILRGMKTMGIRMEQAVKNAQQLANYLAKHPKVLKVHYP
                     GLDSHPNHDTHLQQAKNGGAVLSFELASKEELMTFTHRIQLPILAVSLGGVESILSHP
                     ATMSHACLSSQARLEQGVVDGLLRLSCGVENIEDLLADFEQALAF"
     gene            7679..10180
                     /locus_tag="VGT87_000663"
     CDS             7679..10180
                     /locus_tag="VGT87_000663"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEQ3813541.1"
                     /translation="MTFYDHTAIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRFKRAQGHNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLEDLEEVDWPESIKD
                     MQRNWIGKSTGANVTFKVKDTDKDFTVFTTRPDTLFGATYAVLAPEHALVDAITTADQ
                     AEAVADYKRQASLKSDLARTDLAKEKTGVWTGSYAINPVNGNEMPVWIADYVLASYGT
                     GAIMAVPAHDERDWEFAKQFNLDIIPVLEGGNVEEAAFTEDGLHINSGFLDHLDKASA
                     IAKMVEWLEAEGVGNEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANVTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEELLKQWLPVDIYVGGAEHAVLHLLYARFWHKVLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDSRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEITEENSGALD
                     KVYNETVKAVTEQVDQMKFNTAIAQLMVFVNAANKEDKLFSDYAKGFVQLIAPFAPHL
                     GEELWQALTASGESISYVPWPSYDESKLVENDVEIVVQIKGKVKAKLVVAKDLSREEL
                     QEVALANEKVQAEIAGKDIIKVIAVPNKLVNIVIK"
     gene            10487..11920
                     /locus_tag="VGT87_000664"
     CDS             10487..11920
                     /locus_tag="VGT87_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEQ3813542.1"
                     /translation="MEMLLAPLNWFSQNILQNPAFFVGLLVLIGYLLLKKPIYEVFAG
                     FVKATVGYLILNVGAGGLVTTFRPILVALAKKFELKAAVIDPYFGLAAANTKLEEMGF
                     ISVATTALLIGFGVNILLVALRKVTKVRTLFITGHIMVQQAATISVFVLLLIPQFQNA
                     FGAWAVGIICGLYWAISSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAPFFGKKE
                     ENLDNLKLPTFLNIFHDTVVASATLMLVFFGAILAVLGPDIMSDVDLIGPGAFNPAKQ
                     AFFMYILQTSLTFSVYLFILMQGVRMFVSELTNAFQGISSKLLPGSFPAVDVAASYGF
                     GSSNAVLSGFAFGLIGQLITIALLVIFKNPILIITGFVPVFFDNAAIAVYADKRGGWK
                     AAVALSFISGIIQVALGAVAVGLLGLTGGYHGNIDLVLPWLPFGYLFKFLGIAGYVLV
                     CIFLLAIPQLQFAKAKDKEAYYRGEAQ"
     gene            11991..12269
                     /locus_tag="VGT87_000665"
     CDS             11991..12269
                     /locus_tag="VGT87_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEQ3813543.1"
                     /translation="MVKVLTACGNGMGSSMVIKMKVENALRQLGVTDIQSASCSVGEA
                     KGLASGYDIVVASNHLIHELDGRTKGHLVGLDNLMDDNEIKTKLQEVL"
     gene            12392..12877
                     /locus_tag="VGT87_000666"
     CDS             12392..12877
                     /locus_tag="VGT87_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEQ3813544.1"
                     /translation="MNLKQAFIDNNSIRLGLSADTWQEAVQLAVQPLIDSKAVTSAYY
                     DAIIASTEKYGPYYVLMPGMAMPHAEAGLGVNRNAFALITLTKPVTFSDGKEVSVLLT
                     LAATDPSIHTTVAIPQIVALFELDNAIERLVACQSPKEVLEMVEESKNSPYLEGLDLD
                     S"
     gene            12986..13648
                     /locus_tag="VGT87_000667"
     CDS             12986..13648
                     /locus_tag="VGT87_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEQ3813545.1"
                     /translation="MTHLPNLQVALDHSDLQGAIKAAVAVGHEVDVIEAGTVCLLQVG
                     SELVEVLRSLFPEKIIVADTKCADAGGTVAKNNAKRGADWMTCICCATIPTMEAALKA
                     MKEERGDRGEIQIELYGDWTYEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNKV
                     KTLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAEDPAAAARAFKDEIRRI
                     WG"
     gene            13653..14516
                     /locus_tag="VGT87_000668"
     CDS             13653..14516
                     /locus_tag="VGT87_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEQ3813546.1"
                     /translation="MARPIGIYEKATPKQFTWRERLQFAKDLGFDFVEMSVDESDARL
                     ARLEWTKEERLDLVKAIYETGIRIPTICFSGHRRYPLGSNDPAIEAKSLKLMKQCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPETRARFIKNLRQSCDWAEEAQVMLSIEIMDDPFIN
                     SIEKYLAVEKEIDSPYLFVYPDAGNVSAWHNDLWSEFYNGHKSIAALHLKDTYAVTET
                     SKGQFRDVPFGQGCVDWQELFAVLKKTNYNGPFLIEMWSENCDTVEETKAAIKEAQDF
                     LYPLIEKAGLA"
     gene            14518..15222
                     /locus_tag="VGT87_000669"
     CDS             14518..15222
                     /locus_tag="VGT87_000669"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEQ3813547.1"
                     /translation="MAKNLQEMRERVCAANKSLPQHGLVKFTWGNVSEVCRELGRIVI
                     KPSGVDYDLLTPENMVVTDLDGNVVEGDLNPSSDLPTHVELYKAWPEVGGIVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGPVPCARSLTKAEVDGAYEQETGSVILEEFSKRGL
                     DPMAVPGIVVRNHGPFTWGKTPEQAVYHSVVLEEVAKMNRLTEQINPRVEPAPRYIMD
                     KHYLRKHGPNAYYGQK"
     gene            15547..17193
                     /locus_tag="VGT87_000670"
     CDS             15547..17193
                     /locus_tag="VGT87_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEQ3813548.1"
                     /translation="MMILDKKSYDLLSYLLKLETPETVMAISHALNQSRRKVYYQLDK
                     INQALPKGVDQIISYPRLGILLTADQKAACRLLLEEVTDYNYVMKSDERRRLSSIYIA
                     VSTERVTIDKLMQINDVSRNTILNDLTELREELEDKQYKIQLHATKARGYYFGCHPMA
                     LIQYLYKLLVDVYQGGNTSFIDIFNRKLSEIQGLSVYFSKDILTYFHEYLFLSQASLG
                     KTINTQDSQFMLQILPFMLLSYRNMRLDSETKSALKQEFHLIWKRKEYHIAQDLAREL
                     YHNFKLHLDDIEVSMVAMLMLSFRKDQDHHVESQDYDDMRATISHFIDQLESRYQLHF
                     THKQDLLKQLTTHCKALVYRKAYGIFLVNPLTDHIKEKYEELFAMTQSCATILEQDWT
                     ISLTDDDIAYLTIHLGGELRHNNTEQEKTKLVIVSDDGIGIQKLLFKQCQRYLANGQI
                     EAVFTTEQYQSVYDLLAVDMIVATTDTLKTKIPMLIVNPILSDDDIIKLIRFSKQGRL
                     SEHSRFSTELTKAIEAVVKDESDRYALVSKIEKLIHRELL"
     gene            17446..18537
                     /gene="ulaG"
                     /locus_tag="VGT87_000671"
     CDS             17446..18537
                     /gene="ulaG"
                     /locus_tag="VGT87_000671"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEQ3813549.1"
                     /translation="MTKVQDITRESWILNTFPEWGTWLNEEIEQEVVPADNFAMWWLG
                     NCGIWIKTPGGANVVMDLWSNRGKATKQVKDMVRGHQMANMAGARKLQPNLRAQPMVI
                     DPFMINELDYYLVSHYHSDHIDINTAAAIINNPKLNHVKFVGPYECGEVWKNWGVPKD
                     RIMILKPGDSLEFKDIKITAVESFDRTCLVTLPIQGADAQDGDLAGLAITDDDMARKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGRDYDIDVVLNNYGENPIGIQDKMTSVDLLR
                     MAENLRAKVVIPVHYDIWSNFMASTDEILELWKMRKERLQYDFHPFIWEVGGKYTYPQ
                     DQNRIEYHHPRGFDDCFLEDSNIQFKALL"
     gene            19025..20221
                     /locus_tag="VGT87_000672"
     CDS             19025..20221
                     /locus_tag="VGT87_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEQ3813550.1"
                     /translation="METILEVKHLSKIFGKKQKAALEMVKTGKNKSEIFKKTGATVGV
                     YDASFEVKKGEIFVIMGLSGSGKSTLVRMLNRLIEPSAGSILLEGKDISTMSADQLRE
                     VRRHDINMVFQSFALFPHKTILENTEFGLELRGVRKEERQRLAEKALDNSGLLDFKDQ
                     YPNQLSGGMQQRVGLARALANSPKILLMDEAFSALDPLIRREMQDELLDLQDSMKQTI
                     IFISHDLNEALRIGDRIALMKDGQIMQIGTGEEILTNPANDFVREFVEDVDRSKVLTA
                     QNIMIKPLTTTVELDGPQVALNRMHNEEVSMLMATNRRRQLVGSLTADAAIEARKKGL
                     PLSEVIDRDVRTVSKDTIITDILPLIYDSSAPIAVTDDNNRLLGVIIRGRVIEALANI
                     SDEDLN"
     gene            20237..21964
                     /locus_tag="VGT87_000673"
     CDS             20237..21964
                     /locus_tag="VGT87_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEQ3813551.1"
                     /translation="METILQTKLPVAQLVEQLTEWLTKTFSGLFDIMQVVGSFLMDWM
                     TKTLLFIHPLLFIVLVTAGMFFLAKKKWPLPTFTLLGLLFIYNQGLWKQLMNTFTLVL
                     VASLISVLIGIPLGIWMAKNATVRQIVNPILDFMQTMPAFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLAIRDIPTELIEASDAFGSTGKQKLFKVELPLAKNTIMAGVN
                     QTMMLALSMVVTGSMIGAPGLGREVLSALQHADIGSGFVSGLALVILAIVLDRMTQLF
                     NSKPQEKAKAGKTNKWIGLAALAIFLIAALGRGIMTMASGMADKGETVNIAYVQWDSE
                     VASTHVIAEVLKNEGYHVTLTPLDNAVMWQTVANGNADFSTSAWLPVTHGQQYQKYKS
                     KLDDLGPNLKGTKLGLAVPKYMTDVNSIEDLSKQADQKITGIEPGAGIMAAAKKTLKE
                     YHNLSSWELVAASTGAMTTSLDQAIKKKDPIVVTAWSPHWMFAKYDLKYLKDPKETFG
                     STENINTIARKGLKKDLPNVYKIIDKFHWTQKDMAAVMLDINKGMSPEAAAKKWVEAN
                     KSKVSSWTK"
     gene            22095..24737
                     /gene="polA"
                     /locus_tag="VGT87_000674"
     CDS             22095..24737
                     /gene="polA"
                     /locus_tag="VGT87_000674"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEQ3813552.1"
                     /translation="MENKNKLLLIDGSSVAFRAFFALYNQIDRFKNHSGLHTNAIYGF
                     HLMLDHMMKRVQPTHVLVAFDAGKTTFRTEMYADYKAGRAKTPDEFREQFPYIREMLT
                     ALGIAYYELEHYEADDIIGTLDKMAERTEVPFDVTIVSGDKDLIQLTDENTVVEISKK
                     GVAEFEEFTPAYLMEKMGLTPNQFIDLKALMGDKSDNIPGVTKIGEKTGLKLLHEFGS
                     LEGIYEHIDGFKASKMKENLINDRDQAFLSKTLATINTASPITIGLDDIVYNGPDVAS
                     LSQFYDEMDFVQLKKGLASQMPQEPVAAISYQEVTNVSADLFSAEDIFYFETLRDNYH
                     REAIIGFAWGHGEQIYASTDLSLLATDSFKQVFQKPIATYDFKRSKVLLSHLGIELVA
                     PSYDARLANYLLSTVEDNELSTIARIFTDISLEEDDTVYGKGAKRAVPDKDVLLEHLA
                     RKVKVLLDSKSQMLDKLTAHEQLDLYQNIELPLANVLAKMEIEGIKVNRATLQDMAEQ
                     NKVIIEALTQEIYDMAGQEFNINSPKQLGSILFEKMQLPLEMTKKTKTGYSTAVDVLE
                     RLAPIAPIVAKILDYRQITKLQSTYVIGLQDYILADGKIHTRYVQDLTQTGRLSSVDP
                     NLQNIPIRLEQGRLIRKAFTPSHEDAVLLSSDYSQIELRVLAHISGDEHLIAAFNEGA
                     DIHTSTAMRVFGIDKAADVTANDRRNAKAVNFGIVYGISDFGLSNNLGITRKQAKSYI
                     DTYFERYPGIKAYMENVVREAKDKGYVETLFKRRRELPDINSRNFNVRSFAERTAINS
                     PIQGSAADILKIAMINLDKALQAGGFRAKMLLQVHDEIVLEVPNDELTAIKKLVKDTM
                     EAAVDLAVPLCVDESTGQSWYEAK"
     gene            24924..25379
                     /locus_tag="VGT87_000675"
     CDS             24924..25379
                     /locus_tag="VGT87_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEQ3813553.1"
                     /translation="MIYSFQNPSEDVLKAYLESAKTIAVVGLSDRKDTAAYGVAKFMQ
                     AMDYRIIPVNPKLAGQLILGEKVYASIKAIPFEVDIVDVFRRSEFLPEVARDFLAGQA
                     KVFWAQLGLENQEAQTILRSAGKEAIVMNRCLKIDYLQLVAKPECITNH"
     gene            25431..25898
                     /gene="perR"
                     /locus_tag="VGT87_000676"
     CDS             25431..25898
                     /gene="perR"
                     /locus_tag="VGT87_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HEQ3813554.1"
                     /translation="MDIHSHQQALDAYENVLEHLREKHIRITETRKAIISYMIQSTEH
                     PSADKIYRDLQPNFPNMSLATVYNNLKVLVDEGFVSELKISNDLTTYYDFMGHQHVNV
                     VCEICGKIADFMDVDVMDIAKEAHEQTGYKVTRIPVIAYGICPDCQAKDQPDF"
     gene            26054..26353
                     /locus_tag="VGT87_000677"
     CDS             26054..26353
                     /locus_tag="VGT87_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813555.1"
                     /translation="MFLTWENLFLTNQSRRRLKLLVVERSYNNVGNFLKEYFGGFFEI
                     VNFDDLRLTMVDMVSLSSEYDVIVTDMILEQTTDSEILFFNQMAPSVVANRLTDM"
BASE COUNT         8045 a         4966 c         5680 g         7742 t
ORIGIN      
        1 ccgcattgac aaagactatt tgttaatgta tagtttactt aaaaataata taaggtggtt
       61 tacatgagaa acaaaaaagt aacattagct catattgtcg caaagacaag tgttgctatt
      121 gctttggctg gagcaatggg tagcagttta ttagctaata gcacaacata cgctgttagt
      181 ggcaaagaaa ataaaaaaag cgatgtcaaa tatgaaacga ccaaagttat ggaagctaac
      241 gcaacttcct ctaaagaaga caatcatgtc atgcacacat tagacggctc aatgagtact
      301 gtctgggagg aaaattcacc tggtggtggc gttggtgagg tactttccta caagtttgct
      361 tccccgatgc gtattgggag aattttaatt gttaatggag atacatctag caaggagaat
      421 tactacaaga aaaatagaat tgcaaaggct gatgttaaat actataacgg gaataaattg
      481 gtcctctttc aaaaaattga attaggcgac acctacacta aaaaaccgca tcacattgag
      541 attgataaaa aattagatgt tgatcgtatt gatattgagg taacagaggt ccatcaggga
      601 caaaacaagg atattttagc cttgtcagag gtcacttttg gcaatatgga acgcgatctt
      661 tttgaaaaaa agtttaaaga aattaaagat aaatgggtaa cagataaaca agcagatgag
      721 tttattgaaa ctgccgacaa gtatgctgat aaagctgttc agatgtctgc tgttgcatca
      781 cgtgctgagt attaccggat gtatgttagc cgcaaatacc agtacaaaaa agagtttgtt
      841 gaaaaactaa aacaagtcta caaagaaagc ggagcttccc acgttacaag caaaaaagat
      901 ttgatgttag cttttgatga tgctaaaaga aagtcaacga ttggtcgaca agaaaacggt
      961 ctttttgtga caagttttgc tgaagatatg gcattgctct ttactgatca aggtaagtta
     1021 aaatcagctg accaaattga aaatataaaa ggtgtcgata gcggaagata tagcgatggg
     1081 gtttatcaat atgagtacga ttctgaacta acaaaaaaca ttgataagct aggctatatc
     1141 cgaacagcta gcggagatac ccctggagca aattcgctca acattcctgg ttgccaaacg
     1201 tggtcaggaa aacacattga aaattcagaa agtgaattaa ttttcccatc gattagtgtt
     1261 aaagatctaa aatctaaagc tgtcttagca gagattgatg ccaaaggcta ttttgaaatt
     1321 attgatccta ccatcattgc tccaaatggt gaccataaaa aagtaactgg tcgcttcaaa
     1381 attaagaaaa tgcaagatag aatgcaagat aggaagtaac aatatgtata aggtgccaaa
     1441 gggtttagaa cattaccaaa aaatgtttca aaaagaagta actgttaatg attttaaaaa
     1501 atacttgata ggtagtgaca aagagtatcg tatcactaga agagactctt atatgggaga
     1561 tatttctgat ccagaagtta ttttagagta tggggtttac cctgctttca taaaaggcta
     1621 tacccaattg aaagctaata tcgaagaagc attattagaa atgtcaaata gcggtcaagc
     1681 attagacatt taccaagcgg ttcaaaccct aaacgctgaa aacatgttat taaattatta
     1741 cgaaagcttg ccattttatt taaaccgtca aagcatacta gctaatataa ccaaagcgtt
     1801 aaaagatgcg catattagag aggctatggc acattacaaa ttaggagaat ttgctcacta
     1861 tcaagatact atgcttgata tggtcgaaag aacaatagaa acattttaga atgataaaaa
     1921 ggtatgaagg acatgtctaa taaaaaaaca tttaaaaaat acagtcgcgt cgctgggcta
     1981 ctaacggcag ctcttatcat tggtaatctt gttactgcta atgctgaatc gaacaaacaa
     2041 aacactgcta gtacagaaac cacaacgaca aatgagcaac caaaagcaga aagtagtgag
     2101 ctaactatcg aaaaagcaga tcagaaaatg gatgatatgc ttaactctaa cgatatgatt
     2161 aagcttgctc ccaaagaaat gccactagaa tctgcagaaa aagaagaaaa aaagtcagaa
     2221 gacaaaaaaa agagcgaaga agatcacact gaagaaatca atgacaagat ttattcacta
     2281 aattataatg agcttgaagt acttgctaaa aatggtgaaa ccattgaaaa ttttgttcct
     2341 aaagaaggcg ttaagaaagc tgataaattt attgtcattg aaagaaagaa aaaaaatatc
     2401 aacactacac cagtcgatat ttccatcatt gactctgtca ctgatatgac ctatccagca
     2461 gcccttcagc tggctgataa aggttttacc gaaaacaaac cagacgcggt agtcaccaag
     2521 cgaaacccac aaaaaatcca tattgattta ccaggtatgg gagacaaagc aacggttgag
     2581 gtcaatgacc ctacctatgc caatgtttca acagctattg ataatcttgt taaccaatgg
     2641 catgataatt attctggtgg taatacgctt cctgccagaa cacaatatac tgaatcaatg
     2701 gtatattcta agtcacagat tgaagcagct ctaaatgtta atagcaaaat cttagatggt
     2761 actttaggca ttgatttcaa gtcgatttca aaaggtgaaa agaaggtgat gattgcagca
     2821 tacaagcaaa ttttttacac cgtatcagca aaccttccta ataatcctgc ggatgtgttt
     2881 gataaatcag tgacctttaa agatttgcaa cgaaaaggtg tcagcaatga agctcctcca
     2941 ctctttgtga gtaacgtagc ctatggtcga actgtttttg tcaaactaga aacaagttct
     3001 aaaagtaatg atgttgaagc ggcctttagt gcagctctaa aaggaacaga tgttaaaacg
     3061 aatggaaaat actctgatat cttagaaaat agttcattta cagctgtcgt tttaggagga
     3121 gatgctgcag agcacaataa ggtagtcaca aaagactttg atgttattag aaacgttatc
     3181 aaagacaatg ctaccttcag tagaaaaaac ccagcttatc ctatttcata caccagtgtt
     3241 ttccttaaaa ataataaaat tgcgggtgtc aataacagaa ctgaatacgt tgaaacaaca
     3301 tctaccgagt acactagtgg aaaaattaac ctgtctcatc aaggcgcgta tgttgctcaa
     3361 tatgaaatcc tttgggatga aatcaattat gatgacaaag gaaaagaagt gattacaaaa
     3421 cgacgttggg acaacaactg gtatagtaag acatcaccat ttagcacagt tatcccacta
     3481 ggagctaatt cacgaaatat ccgtatcatg gctagagagt gcaccggctt agcttgggaa
     3541 tggtggcgaa aagtgatcga cgaaagagat gtgaaactgt ctaaagaaat caatgtcaac
     3601 atctcaggat caaccctgag cccatatggt tcaattactt ataaatagga ctggttcaag
     3661 aggttcgtca aacagcttga tgctgcttat ctcttgaggc cttgtgccta ctcctctcac
     3721 aaatacagaa aaaagcttgt ttatgagata ccttcataga caagcttttt tatatactaa
     3781 tcaaaaaatt attcttttct cacatcgtga ccttgaagct aagctgcttc taatctataa
     3841 tactaacaag ctgcttttta acaagcaaag cataacataa aagggagaga taactattat
     3901 catgaaacaa caatcttacc agcctctacg cttcgtctac ctcttggtgg ctctatttgc
     3961 tgctatgttg cttgtagcaa gacctgttat ggcagatgag gagcaaactc ctgaggatag
     4021 agcttatagt caaggtttag aaaaaggtac agaagcaggg agaaaagctg gagaagaagc
     4081 tacttggact gatttgaccc caactgtccc aactgatcta gaaacacctg atgatattga
     4141 agatactaat aaacagcttt ataaagaagg gtatacagat gggtacaaag agggttataa
     4201 tgaaggattt ggagaacact accctttgtt aattcctgtc cagtttatat ggggaatcat
     4261 tttacattgg tgggaaatgt tgagctgaca tttgacaatc aactctaagc tgactccata
     4321 gctagtgggt atagttagac gcagacgaat ttaaaacagt tccttgctac cacaccttaa
     4381 accgcacctt aatcattagg ctttagtgat atgtgtccag gattctgggg acatagccta
     4441 tgtctaattg ttggggtgcg gtttaagtgt ctttgggttt gaagttgtgg caacacccta
     4501 actttaggac agcaaaaaag cccaagcctg cttacgcagt cctgggcttt tttataactg
     4561 ttgtctcact attttgtgac tgtagccgta gaagtagtag taggctgctc tcccatcaac
     4621 aagtgttttg tcttatagaa taaccagctg ataggtccgc aatatccgta aaaatcgtaa
     4681 ttacatgctt gagtagctac tgcctcattt gctgaaacag tttggttttc tggtgttgca
     4741 aggctaaggc ctacaacagc agcagttgcg atcataaata aagataattt ctttttcatt
     4801 tgtaactcct taaattatag tatacatata ttatactcat attatattaa tgattcaata
     4861 tcacatttta atctttttgt aatatgtttg accgaaaaag gcaaagaaag ccaagactaa
     4921 agcatttctt tatcttatat aaatcacatt aattattaat aatattaatc tgattatcat
     4981 tctatttgag gtgttacagc ttttttggtg ctggttagct agcaactgtt acgacttgtg
     5041 ttttgggctt ctctcttttt ataggcattg tgccttttct gttttacctt gaggggtcat
     5101 tggcaaaaac gttttagcca atcaaaaagc ctaaggctgc ttatgcagcc ctaggctttt
     5161 cataaccgta gtcttacgaa gaagatagct tctatgttac ggtatgaggt ttaccatact
     5221 gtgtaaagta accagctctc aggaccattg tgtccgtaga aaccgttact atagttagca
     5281 tcatttgctg aaacagtttg gtgctctcct agtgttgcaa ggctaaggcc tgcaacagca
     5341 gcagttgcga tcataaataa agataatttc tttttcatag ataactcctt taatttgtag
     5401 tatgactata ttatactaac tttttgttaa taaatcaagt attattttaa tgtttttatt
     5461 attattcatc tgaataagaa atgtggcgac ctaggattag aaaaccgcca cgagatggtt
     5521 ttttatttta atagttagcg gtagactatg ctgtgactag tattacgaaa aataacctgt
     5581 tatttttgac caccaattgg taactatctc ccaaaaccag ttaagaaggg aaaaatcgtt
     5641 attttttgtg ataatgtttt cttctattgg catgccatct tttctctcga agcaaacacc
     5701 tacataagga ttctcattag agtatgtaca tgttcctgcc agtgcctgct cagccgcatg
     5761 cacggtctgg tgatgagggg ttgctaaaga cggccctagg acagttacgg tcgcaagggc
     5821 aactaaaaat aatttgtttt tcactatatt tctctcctaa aaataaatag tactttagca
     5881 gtgtactata aggcgcatgg tgaaaacaag ttctgtttct cattaaatga aataaattgt
     5941 gaccttttaa tgaaattttg atggcaatat tgccctattg agtaggtgac gatgcaaagt
     6001 aggactgtca gtgtgataag ctaagctttt gagacgatgg ggccaaaggt agctatactt
     6061 taacttgcta gagaggtgtt ggcagcttgg tcaaagatga taaaagggct tgcaaactta
     6121 gctaaaggtt gtataataaa cgtaatcgat ctaaaaatga tattgttgga aaaattcatt
     6181 atccatttaa aaggtgccca ctacttttct tattaggtta gcagatcacg cttttttgaa
     6241 gagctttttg gtttgtgcaa ccattggggt gggaccattg tctaaaagag agttcattgg
     6301 agttaacgct attgcggagg tgcacgaaat gagagaaaat acgaccttat tacatggcta
     6361 tacggttatt gacgagttta caggagcagc ttcagttccc atctatcaga cttcgacgtt
     6421 ccacaattca gaactgtatt gtcctagtca aaaacatttg tacacacgct tttcaaaccc
     6481 aacgactgaa gctttagaag acgggttagc ctgtttagaa aaagcgactt atgcggtggc
     6541 ttatgcatct ggtatggctg ctatttcaac tgttttgatg ctcttaaaag ctggagatca
     6601 tgtgattttc ccattggagg tctatggagg gacttgccaa tttgcgacag ctattttacc
     6661 caattatcag attgaaacaa gctttgtaga tatggctgat ttagcaacag taaaagcaag
     6721 tatcaggcct aatacgcgta tgatttatct agagacaccg tcaaatccgc tcttgaaaat
     6781 ttgtgatatt tcagagcttg tgcaactcgc caaagcttat ggtgtgctaa ctgtggctga
     6841 taataccttt atgacaagtt tgtatcaaga acccttagct atgggtgtgg atattgtggt
     6901 agaaagtgtg acaaaattta tcaatggtca tagtgatgtg gtagctggtt tagctgctac
     6961 caataatgag gctatctaca accagctcaa gctttttcaa aaaaactttg gagcaattgt
     7021 tggggtcgaa gacgcttggc tgattcttcg tggcatgaaa actatgggga tccgtatgga
     7081 acaagcggta aaaaatgctc agcagctcgc taactattta gcgaagcacc ccaaagttct
     7141 taaggtacat taccctggct tagacagtca tcctaatcac gatacccacc tgcaacaagc
     7201 taagaatggt ggtgctgttt tatcctttga gttggcttct aaggaggagc tgatgacctt
     7261 tacccatcgt attcagttgc cgattttagc cgttagtcta ggtggagtag agtccatttt
     7321 gtcacatcca gcaacgatgt ctcatgcttg tttatcatca caagcgcggt tggaacaagg
     7381 ggttgttgat ggtttgctgc gcttgtcttg tggtgttgaa aatatcgaag acttattggc
     7441 cgattttgag caagcacttg ctttttaagt ctatttgtcc agattggctc tgtttacaga
     7501 gcaagtctgg cttttttatt agctttttgt gaggatggtt gatgctaaag cagggtttaa
     7561 aaatagctaa tcgtctgaag tctcaaaagc caaggtgcta gtcatgtctt gaccattcct
     7621 aaaagcttca tctcatgcta taataagaag actagatttt ttacatggga gatttcacat
     7681 gacattttac gatcacaccg ctattgaacc caaatggcaa gccttttggg cagataatca
     7741 tacctttaaa acaggaacag acgcatcaaa acctaagttt tatgccctag atatgttccc
     7801 atacccatca ggagctggtc tccacgttgg tcacccagaa ggctatacag caactgatat
     7861 tctcagccgt ttcaaacgtg cccaaggtca caatgtcctc cacccaatgg gttgggatgc
     7921 ttttggactt cctgctgagc agtacgctat ggatactggt aatgacccag ctgagtttac
     7981 agctgaaaac attgctaact ttaagcgcca gattaacgct ctcggattct catacgactg
     8041 ggatcgcgaa gtcaacacaa ctgaccctaa ctactacaag tggacacagt ggatctttac
     8101 taagctttat gaaaaaggcc tagcctacga agctgaagtt cccgttaact gggttgaaga
     8161 attgggaaca gctatcgcca acgaagaggt tctccctgat gggacatcag aacgtggcgg
     8221 ctacccagtt gtcagaaaac ccatgcgcca atggatgctc aaaatcactg cctatgcaga
     8281 gcgcttgcta gaagacttag aagaagttga ctggccagaa tcaatcaagg acatgcaacg
     8341 caactggatt ggtaaatcaa ctggtgctaa tgtaaccttc aaggttaagg atacagataa
     8401 ggacttcact gtctttacaa cacgtcctga cacccttttt ggtgcaactt acgccgttct
     8461 tgctcctgag catgccttgg ttgatgctat tacaacagct gaccaagcag aagccgtggc
     8521 tgactacaaa cgtcaagcaa gtctcaaatc agaccttgct cgtacagacc ttgccaaaga
     8581 aaaaactggt gtctggacgg gtagctatgc gattaaccca gttaatggta acgaaatgcc
     8641 agtttggatt gcggactatg tccttgccag ctacggtact ggtgctatca tggctgttcc
     8701 agcccacgat gaacgtgact gggaattcgc aaaacaattt aacctagata tcattccagt
     8761 tcttgaaggt ggtaatgttg aagaggctgc ctttacagaa gacggtcttc acatcaactc
     8821 aggcttccta gaccatcttg acaaagcgtc agctattgcc aaaatggtcg aatggcttga
     8881 agcagaaggc gtcggtaatg aaaaagtcac ttaccgtctc cgtgactggc tctttagccg
     8941 tcaacgttac tggggtgagc caatcccaat catccactgg gaagatggga cctcaacagc
     9001 ggttcctgag agtgagttgc cactggttct tccagtaact aaggatattc gtccttcagg
     9061 tactggtgag agcccacttg ctaacgtgac agactggttg gaagtgactc gtgaagatgg
     9121 tgttaaaggt cgtcgtgaga ctaacaccat gccacaatgg gcaggttcaa gctggtacta
     9181 tctccgttac atcgatccac acaacaccga aaaactagct gatgaggaac tcctcaaaca
     9241 atggttgcca gttgatatct atgtcggtgg tgcagagcat gccgtgcttc accttctcta
     9301 cgctcgcttc tggcacaagg ttctttacga tctcggtgtc gtaccaacca aagagccatt
     9361 ccaaaaactt tttaaccaag ggatgatttt gggaacaagc taccgcgaca gccgtggagc
     9421 gcttgtagcg actgacaagg ttgaaaaacg tgatggcagc ttcttccatg tggaaactgg
     9481 tgaagagctt gaacaagcac cagctaagat gtctaaatca cttaagaacg ttgtcaaccc
     9541 agatgatgtt gttgagcagt acggtgctga cacccttcgt gtttatgaga tgttcatggg
     9601 accacttgac gcttccatcg cttggtctga agaaggtctt gaaggctcac gtaagttctt
     9661 ggaccgtgtt taccgtttga tcacgtctaa agagattact gaggaaaata gcggagccct
     9721 tgacaaggtt tacaacgaaa ctgttaaggc cgttactgag caagttgacc aaatgaaatt
     9781 caacacagct atcgctcaac tcatggtctt tgtcaatgca gccaacaagg aagacaaact
     9841 ctttagcgac tatgccaaag gctttgttca attgattgca ccatttgcac cgcacttagg
     9901 tgaggaactc tggcaagcct tgacagcctc aggtgagtca atctcttacg tgccatggcc
     9961 aagttatgat gaaagcaaat tggttgaaaa cgatgtggaa atcgttgtcc aaatcaaagg
    10021 taaggtcaaa gctaaacttg tagttgccaa agacctttca cgcgaagaat tgcaagaagt
    10081 tgcccttgct aacgaaaaag tccaagccga aatcgcaggc aaagacatca tcaaagtcat
    10141 cgcagtacca aacaaactcg ttaatattgt tatcaaatag tttgacttga cacagccctt
    10201 cagtggttat ttgcgtcacg tcacagttaa tgccaaagag ctgatctatt caaaaagcaa
    10261 ctcatctaga aaaaccacaa gaaccttcgt ttttgtggtt tttaatcgtt gtttaggggt
    10321 atttcctcct tattattaag tgtagttaaa ttttaaaata agttaactta agtctaatat
    10381 tgaacacttc tttgtgtgat agttggtctt ttttaaaacg ctttcattac ctataatgaa
    10441 catataaaca ctttatcttt attaactttg aaaagaggta gttcttatgg aaatgctcct
    10501 agctccccta aactggttct cgcaaaatat tttgcaaaac ccagcgttct ttgtaggtct
    10561 tttggtttta atcgggtatt tactccttaa aaaacccatt tatgaggtct ttgctggttt
    10621 tgtaaaggct actgttggtt acttgatttt gaacgttgga gcaggcggtc ttgtaacgac
    10681 ttttcgtccg atcttggttg ctcttgccaa gaaatttgaa ttgaaagccg ccgttattga
    10741 tccttatttt ggtttggctg ctgctaatac taagttagaa gagatgggct tcatcagtgt
    10801 tgctacgaca gctctcttga ttggatttgg ggtcaacatt cttttggttg cacttcgtaa
    10861 ggtcactaaa gttcgtactc ttttcatcac aggccacatc atggtccaac aggcagcgac
    10921 tatctctgtc tttgtccttc ttttaatccc tcaattccaa aatgcttttg gggcgtgggc
    10981 agttggtatc atctgtgggc tttactgggc tatcagttct aatatgaccg ttgaagcaac
    11041 tcaacgcctt acaggtggcg gtgggtttgc gattggtcac caacaacaat ttgcgatttg
    11101 gtttgtggat aaggttgcac cgttctttgg taaaaaagaa gaaaaccttg ataacctaaa
    11161 attaccaacc ttcttaaaca ttttccatga tactgttgtt gcatcagcaa ctttgatgtt
    11221 ggtctttttc ggagctatct tggctgtcct tggtccagac attatgtctg atgtggactt
    11281 aatcggacct ggagcattca acccagctaa gcaagctttc ttcatgtata ttttgcaaac
    11341 ctctctcacc ttctcagttt atctctttat cttgatgcag ggtgttcgca tgtttgtttc
    11401 tgaattgacc aatgcctttc aagggatctc aagcaaattg cttccagggt cattcccagc
    11461 ggttgacgtt gcagcttcct atggctttgg ttcttcaaat gcggtgcttt caggatttgc
    11521 ctttggtttg attggtcaat taatcaccat tgctcttctt gttatcttta aaaatccaat
    11581 cttaattatc acaggattcg ttcccgtatt ctttgataat gcggcaattg cggtttatgc
    11641 tgataaacgt ggtggctgga aagcagcggt agctctatca tttatctcag gtattattca
    11701 ggtggcactt ggtgctgttg ctgtaggtct tttaggactt acaggtggtt atcacggtaa
    11761 tatcgacttg gttcttccat ggttaccatt tgggtacctc ttcaaattcc ttggtattgc
    11821 agggtatgtg cttgtttgca tcttcttact tgccattcca caattgcagt tcgcaaaagc
    11881 taaggacaaa gaagcttact accgtgggga agctcaataa cactgcatct taagaaatag
    11941 ctctgctatt ttaacaaaac aataataaaa cattttcgga ggaaaaaaca atggttaaag
    12001 tcttaacagc atgtggcaat ggtatggggt catctatggt gatcaaaatg aaagttgaaa
    12061 atgcgcttcg tcaacttggt gtgacagata ttcagtcagc ttcttgctca gttggtgaag
    12121 ctaaaggtct agcttctggc tatgatattg tagtggcatc aaaccacttg attcatgagc
    12181 ttgatggtcg taccaaaggc cacttggttg gtcttgataa cctgatggat gataatgaaa
    12241 ttaaaaccaa actacaagaa gtgctataag ctaattctga atcaaaacga aggagttgtg
    12301 acgaaggttt tcagctcttt ctctttagta ggataaaact agtaaaaatg gtgtgtgttg
    12361 caaagcaaga cactagaaag agatagtttt tatgaattta aaacaagcct ttattgacaa
    12421 caactccatc cgattggggc taagtgctga tacttggcaa gaggctgttc aattagctgt
    12481 tcaacctttg attgatagca aagcagtgac gtcagcttac tacgacgcca tcattgcatc
    12541 aacagaaaaa tacggccctt attatgtgtt gatgccaggt atggctatgc cccatgcaga
    12601 agcaggttta ggcgttaatc gcaatgcatt tgctttgatt acactaacaa aaccagttac
    12661 cttctcagat ggtaaagagg tttctgttct tttgacgttg gccgctacag acccttctat
    12721 tcacacaaca gtggctatcc cacaaattgt ggccttgttt gaactagata acgccattga
    12781 acgcttggtg gcctgccaaa gtcctaaaga ggttctagaa atggtagaag aatcaaaaaa
    12841 tagcccgtat ttagaaggtt tggatttaga ctcttaattg atagcttact gacttaaacg
    12901 gataaaacga ctcacaatca ttattgaaag gataagaact cctcatcagc tttttcatag
    12961 ccgctgagag ttctaggaaa aacacatgac acatcttcca aatttacaag ttgccttaga
    13021 ccattctgac ttgcaggggg ctatcaaagc agccgttgct gtgggtcacg aagtagatgt
    13081 gattgaagct ggtaccgttt gtttgcttca agtgggaagc gaactagttg aggtattacg
    13141 cagcctcttc ccagagaaaa ttatcgtagc agataccaaa tgtgctgacg ctggcggtac
    13201 ggtcgctaaa aacaatgcaa aacgtggtgc tgactggatg acctgcattt gctgtgcgac
    13261 gattccaacc atggaagcag ccctcaaggc tatgaaagaa gagcgcggtg accgtgggga
    13321 aattcaaatc gaactttatg gtgattggac ttatgagcaa gctcaattat ggcttgacgc
    13381 tgggatttcc caagcgattt accaccaatc tcgtgatgcc cttcttgctg gagaaacttg
    13441 gggtgaaaaa gaccttaaca aggtgaaaac attgattgac atgggcttcc gcgtgtcagt
    13501 aactggtggg cttgatgtag acactttgaa actttttgaa ggggttgatg tctttacctt
    13561 tatcgcaggg cgaggcatta ctgaagcaga agatcctgct gcagcggcgc gtgccttcaa
    13621 agatgaaatc agacgtatct gggggtaact ttatggcgcg tcctattggt atttatgaaa
    13681 aagcaacgcc aaaacagttt acctggcgtg aacgcttgca atttgcaaaa gacttaggtt
    13741 ttgattttgt ggaaatgtct gttgatgaat cagatgctcg cttagcgcgc ttagagtgga
    13801 ctaaggaaga acggcttgat ttagtgaaag ccatttatga aacaggcatt cgcatcccga
    13861 ccatttgttt ttctggtcat agacgttacc cactaggctc aaatgatcct gctattgaag
    13921 ccaagtccct taaactgatg aaacaatgta ttgaattggc acaggactta ggcgttcgta
    13981 ccattcaatt agctggttat gacgtttact atgaagaaaa atcgcctgaa acccgtgctc
    14041 gttttatcaa aaacttgaga caatcttgtg actgggccga agaagcccaa gtgatgcttt
    14101 ctattgagat tatggatgac ccttttatca actctattga aaaatacttg gcagttgaaa
    14161 aagagattga ctcgccttat ctctttgttt atcctgatgc aggcaatgtt tctgcctggc
    14221 ataatgattt gtggagtgaa ttttacaacg gtcacaagtc cattgcagcc ctccacttga
    14281 aagatactta tgctgtcacg gaaacctcaa aagggcagtt tcgtgatgtg ccatttggtc
    14341 aaggttgtgt ggactggcaa gagctgtttg cggtcctcaa gaaaaccaac tacaatgggc
    14401 cttttttgat tgaaatgtgg tcagaaaatt gtgacacggt ggaggaaaca aaagctgcta
    14461 tcaaagaagc tcaggacttc ctttacccac ttattgagaa agcggggttg gcataatatg
    14521 gcgaaaaact tgcaagaaat gcgtgaacgt gtctgtgcgg ctaacaagtc tctaccacaa
    14581 cacggtcttg tcaaatttac gtggggcaat gtctctgaag tttgtcgtga attaggacgt
    14641 attgtcatca agccttctgg ggtggactac gacctcttga caccagaaaa tatggtggtg
    14701 acagaccttg atgggaatgt ggtcgaaggt gatttgaacc cttcttcaga tttgccaacc
    14761 catgtggaac tttataaagc ttggccagaa gtgggcggta ttgtccacac tcactccaca
    14821 gaagctgtcg gctgggcaca ggcaggtcgt gatattccgt tttatgggac tacccatgct
    14881 gactattttt atggcccggt tccttgtgct cgctctctca caaaagcaga agttgatggg
    14941 gcttacgagc aagaaactgg tagcgtgatt ttagaagagt ttagcaagcg aggtcttgac
    15001 ccgatggcgg ttccaggaat cgtggtgcgt aatcatggcc catttacgtg ggggaaaacc
    15061 ccagagcaag ccgtttacca ctcggttgtc ttagaagaag tggctaaaat gaatcgcttg
    15121 acagaacaaa tcaatccacg cgtagagcca gcgccaagat acattatgga taagcattat
    15181 ttgcgtaaac atggcccaaa tgcttattac ggtcaaaaat gaggtcgcga tatggcttat
    15241 tagcacaggt tctcactaga agccagaaag ttcaaggttt tgttaatcat tgtttgggca
    15301 agtccttttg acttgtcctt ttcgatgaac tgtttaaacg ccaatgagca caaactggat
    15361 gaatcgtatg ttgtggagtt cttagagacc agatataagg tgttggtgtt ggatcagttg
    15421 aggtgattag ctcgaatcat attaagcgaa tcttattatg gtgagttttt caaaacttgg
    15481 ctgcgcagtc actgaaatca tgataaaata agcaaggatg gatttttaga gaggaggttt
    15541 gtaaacatga tgattttaga taaaaagagt tatgacttgc tgtcttattt acttaaacta
    15601 gaaacacctg agacagttat ggccatttct catgctttaa atcagtcacg tcgtaaagtt
    15661 tattaccagc ttgacaaaat taatcaggct ttgccaaagg gagtagatca gattatctct
    15721 tatcctagac ttggcattct tttaacagct gatcaaaaag ctgcttgtcg cttattatta
    15781 gaagaagtga ctgattataa ttatgtcatg aaaagtgatg agcgcagacg cctatcctcg
    15841 atttatattg cggtttccac tgagcgcgtg accattgata aactgatgca gatcaatgat
    15901 gtgtctcgta acacgatttt aaatgacctg actgagttaa gggaagaact ggaagacaaa
    15961 caatacaaga ttcagcttca tgcgacaaag gctaggggat attattttgg ttgtcatcca
    16021 atggctctta ttcaatacct gtacaaattg ttagtggacg tttaccaagg tggcaatact
    16081 agctttattg atatattcaa ccgtaaactc tcagagatac aaggcttatc ggtttatttt
    16141 tctaaagata ttttgactta ttttcatgag tatctctttt tatctcaagc tagcttagga
    16201 aagacaatta atacccaaga tagccaattc atgttgcaga ttttaccttt catgctttta
    16261 agttatcgta atatgcgtct tgattcagaa acaaagtctg ctcttaaaca agaatttcat
    16321 ttgatttgga aacgtaaaga atatcacatt gctcaggatt tagctagaga gctttaccat
    16381 aattttaagc ttcatttgga tgatatcgaa gttagtatgg tagcgatgtt gatgttgtct
    16441 tttcgaaaag atcaagatca tcacgtggaa agtcaagatt atgacgacat gcgagcaacc
    16501 attagtcact ttattgacca gttggagagc cgttatcagt tacatttcac gcataaacag
    16561 gatttgctga agcagttgac gacccactgt aaggcgctcg tttaccgtaa agcttatggg
    16621 atttttttag ttaatccctt aacagaccat attaaagaaa aatacgaaga attgtttgct
    16681 atgactcaat cttgtgccac tattttggaa caagattgga cgattagctt gacagacgat
    16741 gatattgcct acttgactat ccatcttggg ggagagctac gtcacaataa tacagagcaa
    16801 gaaaagacca aactcgttat tgtttcagat gatgggatag gcattcaaaa gttattgttc
    16861 aaacagtgtc aaaggtatct agcaaatggt cagattgaag ctgtttttac gacagaacag
    16921 tatcagagtg tttacgacct tttagcagtc gatatgattg ttgctactac agatacgcta
    16981 aagactaaaa tcccgatgct tattgttaac cctattttaa gtgacgatga tatcatcaaa
    17041 ttgatacgct tttcaaagca aggacgcttg agtgagcact cccgcttttc tacagagctc
    17101 acaaaagcta ttgaagctgt tgtaaaagat gaatcagatc gttatgcctt ggtttctaaa
    17161 attgaaaaac tcattcatcg cgagctgcta tgagtaagtg ttaagtcgtc aaagtacctc
    17221 agttttctat gacatagaaa aatataactg aggtttttaa tatagctttg aatacagtat
    17281 tgatgcgaga tacagtgtgc aaaatgagac agtattagac tgagctaatg aaaaagtcag
    17341 tccacttttg aacacagatc tgtgtgacac ttggtctttt tatcattgac aagaaagtct
    17401 atacttaaag tatagaaacg aagaacaaat tcggaggaaa ccctaatgac taaagtacaa
    17461 gatattacac gtgaatcatg gatccttaac acgtttccag aatggggaac atggttaaat
    17521 gaagaaatcg agcaagaagt cgtgccagct gacaactttg cgatgtggtg gttaggaaac
    17581 tgtggtatct ggattaagac accaggcggt gctaatgttg tcatggactt atggtcaaat
    17641 cgtgggaaag cgaccaagca agttaaggat atggttcgtg gacatcaaat ggctaatatg
    17701 gcaggagctc gtaaattaca gcccaatctt agggcccaac caatggtgat tgatccattt
    17761 atgattaatg agttggatta ttatttggtt tctcattatc atagtgacca catcgatatc
    17821 aatactgcgg cagccattat caataaccca aaacttaatc atgttaaatt tgtaggacct
    17881 tatgaatgtg gtgaagtttg gaaaaattgg ggtgtgccaa aagatcgtat tatgatctta
    17941 aagccaggtg atagccttga attcaaagat attaagatca ccgctgtaga gtcgtttgac
    18001 cgcacctgtc tggtaactct cccaatacaa ggtgctgatg ctcaagatgg tgatttagca
    18061 ggtttagcta tcacagatga tgacatggct cgcaaagctg ttaactatat ttttgaaaca
    18121 ccaggtggta ccatctatca tggtgcggat tctcattttt cgaactactt tgctaaacat
    18181 ggacgtgatt atgatattga cgttgtgtta aataactatg gggaaaaccc aattgggatt
    18241 caagacaaga tgacttctgt tgaccttctt cgtatggcag aaaatctccg tgctaaagtc
    18301 gttatcccag ttcattatga catttggtca aactttatgg catcaacaga tgaaatcctt
    18361 gaactgtgga aaatgcgcaa agaacgtctc caatacgatt tccatccctt tatctgggag
    18421 gttggtggca agtacaccta tccacaagac caaaatcgta tcgaatacca tcatcctcgt
    18481 ggatttgatg actgcttctt agaagattct aatatccaat ttaaagcttt actataagca
    18541 acaccctact cacctaagat gatagtgcct gattaagcta actcgcttcg taggacactg
    18601 tcttaggtga gtttttttgg agagatgagc aaatgtttaa gagtcaccgt ggtcgttatt
    18661 tacgatggcc gcaaaagaga tattataggt aaaagaagga tttctaatgt aacaatagcc
    18721 taatcagcaa agaaatagtc atcagttgtg ataaaaaatt gtactggtta aaagaccata
    18781 cctaaaagaa tgaaagcgat cacctatcat tgtggcgtta ccagatcgag ttattgtcct
    18841 tgacaaaaag tgaccacacg tgataatata gttatgttct gtttcggcag attctttttt
    18901 taagtaaaaa agtgaccaca cgtttattaa ttgtgtagag ctactttgtt aaatattggc
    18961 taatatttcg ccaaagctat gtcactattt cacatgataa acgattacct aggagaaaaa
    19021 acgaatggaa acaattttag aagtcaaaca tctcagtaaa atttttggca aaaaacaaaa
    19081 agcagctctt gagatggtaa agactggcaa aaataagagc gagattttta agaaaacagg
    19141 cgctactgta ggtgtctatg acgctagttt tgaggtcaaa aaaggtgaaa tctttgttat
    19201 tatggggcta tcaggaagtg ggaaatcaac ccttgtccgc atgctaaatc gtttgattga
    19261 accttcggca ggatctatct tgctggaagg taaagacatc tcaaccatgt cagcagatca
    19321 gctgcgtgag gtgcgccgcc atgacattaa catggttttc caaagctttg ccctctttcc
    19381 tcataaaacc attttggaaa ataccgaatt tggtttggaa ttacgtggcg ttcgcaaaga
    19441 agaacgccag cgattggcag aaaaagccct tgataattca ggcctattag attttaaaga
    19501 ccagtaccca aaccaactat ctggtgggat gcagcagcgt gtcggcctag cccgtgcgct
    19561 agctaatagt cctaaaattc tcttaatgga tgaggcattc tcagcgcttg atcctttgat
    19621 tcgtcgtgag atgcaagatg aattacttga tttgcaagat agcatgaaac aaaccatcat
    19681 ctttatcagt catgacttga atgaagcttt gcgtattggt gatcggattg ctttgatgaa
    19741 agacggacaa attatgcaaa ttggtactgg tgaggaaatc ttgactaacc cagccaatga
    19801 ctttgtgcgt gaattcgttg aggatgtgga ccgttctaaa gtcttgacag cacaaaatat
    19861 catgatcaaa ccgttaacaa ctactgttga attagatgga cctcaagttg ccttgaaccg
    19921 tatgcacaac gaagaggtgt ctatgttgat ggcgacgaat cgccgccgcc aattagtcgg
    19981 tagtttgacg gccgatgccg ctatagaggc gcgcaaaaaa gggttaccgc tatcagaagt
    20041 gattgatcgc gatgtgagaa ctgtctcaaa agatactatt attacagata ttttgcctct
    20101 tatctatgat tcatctgctc cgattgcagt gacagatgat aataatcgtc tgttaggtgt
    20161 cattattcga ggacgagtga ttgaagcctt ggctaatatc tcagacgaag accttaacta
    20221 aaaggagtga tgcattttgg aaacgatatt acaaacaaaa ttaccagtag ctcaactggt
    20281 ggagcagcta acggagtggc taactaaaac tttctcaggt ctatttgata ttatgcaagt
    20341 agttggtagc tttttgatgg attggatgac caaaacactt ttatttatcc acccgttact
    20401 ttttattgtt ctagtaacag cagggatgtt tttccttgct aagaaaaaat ggcctttgcc
    20461 aacctttacc ttgttaggat tgctctttat ttacaatcaa gggttatgga agcagctaat
    20521 gaataccttt actctggtgt tagtagctag tttgatttcg gtacttattg gtattccgct
    20581 ggggatttgg atggctaaaa atgccactgt ccgtcagatt gtgaatccta ttttggactt
    20641 tatgcaaacc atgccggctt ttgtttactt gattcctgcc gttgcctttt ttggtattgg
    20701 tatggtgcct ggagtatttg cttcagtcat ctttgccttg cccccaacag ttcgttttac
    20761 aaatttggcc attcgtgaca ttccaacaga attgattgag gcctcagatg cctttgggtc
    20821 aactgggaaa caaaagctct ttaaagttga attgccttta gctaaaaata ctattatggc
    20881 aggtgttaat cagaccatga tgttagccct ttccatggta gtaacaggct ccatgattgg
    20941 tgctcctggt ttaggacgtg aagtcttgtc agcccttcaa cacgctgata ttggtagtgg
    21001 atttgtcagt ggtcttgcct tggttatctt ggcgattgtg cttgaccgta tgacacagtt
    21061 gttcaatagt aaacctcaag aaaaagccaa agctggcaaa accaataaat ggattggtct
    21121 agcagccctt gctatctttt tgatagctgc tttaggacgt ggtattatga ctatggcttc
    21181 gggtatggca gataagggtg aaactgttaa catcgcttac gtgcagtggg attctgaggt
    21241 tgcctcaact catgtgattg cagaagtttt gaaaaacgaa ggttaccatg tgaccttgac
    21301 tccgcttgac aatgctgtca tgtggcaaac cgttgctaat ggcaacgctg acttttcaac
    21361 tagtgcttgg ttgccagtca cccatggcca acaatatcag aaatacaagt caaaattaga
    21421 tgatttagga cctaacctaa aagggaccaa acttggttta gctgttccaa aatacatgac
    21481 agatgtcaat agtattgagg acttaagcaa acaagccgat caaaaaatca cagggatcga
    21541 accaggtgca ggtatcatgg cagcagccaa aaagactcta aaagagtatc ataatctatc
    21601 tagttgggag ttagtggcgg catcaacagg agccatgacg acttccttgg atcaagcgat
    21661 caagaaaaaa gatcctattg tcgtgacagc ttggtcacca cactggatgt ttgccaagta
    21721 tgacttgaag taccttaaag atcctaaaga aacctttggc tcgactgaga acatcaatac
    21781 cattgctcgt aaaggcttga aaaaagatct tccaaatgtc tacaaaatca ttgataagtt
    21841 ccactggacc caaaaagata tggcagcagt catgcttgac atcaataaag gcatgagtcc
    21901 agaagctgcc gccaaaaaat gggtagaggc aaacaaatct aaagtatcaa gttggacaaa
    21961 atgagtgctt ttgtctaaat agcaaataga gaagtctagg aattttccta ggctttttag
    22021 gttaaccatt tacttatcag caaaaccaat ggcttaaagg agaaaagccc gtccctctgt
    22081 gttataataa gcttatggaa aacaagaata aattactatt aattgatggg tcatctgttg
    22141 cctttcgtgc cttttttgcc ctttacaatc aaattgaccg ctttaaaaac catagcggtc
    22201 ttcataccaa tgccatttat ggctttcatc tgatgctaga ccacatgatg aaaagagtcc
    22261 agcctactca cgtgctggtg gcttttgatg cgggcaaaac caccttccga acagaaatgt
    22321 atgccgatta taaggcgggt cgagctaaaa ccccagatga atttcgtgag cagtttcctt
    22381 atattcgtga gatgttgaca gcccttggga ttgcttatta tgagttggaa cactacgaag
    22441 cagacgatat tatcgggacg cttgataaaa tggcggagcg cacagaagtg ccttttgatg
    22501 tgaccattgt cagtggtgat aaagatttga tccagttaac tgacgaaaat accgtggttg
    22561 aaatttctaa aaaaggggtt gccgaatttg aagaattcac accggcttac ctcatggaaa
    22621 aaatgggctt gacgccaaac cagtttattg acctcaaagc cttgatggga gataaatcgg
    22681 ataacattcc tggagtgaca aaaattggag aaaaaacagg ccttaagctc cttcatgaat
    22741 ttggtagttt ggaaggcatt tacgagcata ttgatggatt caaggcctca aaaatgaaag
    22801 aaaacttgat taatgaccgt gaccaagctt tcctatctaa aacgctcgcc actatcaata
    22861 cagcatctcc tatcacaatt ggcttagatg atatcgtcta caatggtcca gatgttgcta
    22921 gcctctctca attttacgat gagatggatt ttgtgcaact caagaaaggt cttgctagcc
    22981 agatgcctca agagccagta gctgccattt cctatcaaga agtgaccaat gtgtctgctg
    23041 atttgtttag cgcagaagac atcttctact ttgaaacgct tcgggacaat taccatcgtg
    23101 aggccatcat tggttttgct tggggacatg gtgagcagat ttacgcatcc actgacctta
    23161 gcctattagc gacggatagt tttaagcagg tcttccaaaa acccattgcc acttatgatt
    23221 ttaaacgtag caaggttttg ttgagccact taggaattga gttggtagca cctagctatg
    23281 atgctagatt agctaattac cttctgtcaa ctgtagaaga caatgagctt agcaccattg
    23341 cccgcatttt tactgacatc tctttggaag aagacgatac agtctatggt aaaggtgcta
    23401 aacgggctgt tcctgataaa gacgtcttat tagaacactt agctcgtaag gtcaaagtat
    23461 tattagatag caagtctcaa atgctagaca agctcactgc ccatgagcaa ctagacctct
    23521 atcagaatat tgaattgcct ttggctaatg tccttgctaa aatggaaatt gaagggatca
    23581 aggttaatag ggcgactttg caagatatgg cagagcaaaa caaggtcatt attgaagcgc
    23641 tcacccaaga gatttatgat atggctggcc aagaatttaa cattaattcg cctaaacagc
    23701 tgggcagtat cttgtttgag aagatgcaac tacctcttga gatgactaag aagaccaaaa
    23761 caggttattc tacagcagtg gatgttttgg aacgtttggc accgattgct ccaattgtgg
    23821 ctaaaatttt ggattaccgc cagattacta aattgcaatc cacttatgtg attggtttac
    23881 aggattatat tctggctgat ggcaagattc atacccgcta tgtgcaggat ctcacccaga
    23941 cagggcgttt gtctagtgtg gaccctaatc ttcaaaacat tcctattcgt ttggaacagg
    24001 ggcgtttgat tcgtaaggcc tttaccccat ctcatgaaga tgccgtcttg ttgagttcag
    24061 attactcaca gattgaattg cgggtactgg cacatatttc aggggatgag catttgattg
    24121 ctgcctttaa tgaaggggca gacattcata cctcaacggc tatgcgtgtg tttgggattg
    24181 acaaggcagc agatgttact gccaatgacc gccgtaatgc caaggctgtt aactttggaa
    24241 ttgtgtatgg gatttctgat tttggtttgt caaataacct tggtatcacc cgtaaacagg
    24301 ccaagtctta cattgatacc tattttgaac gctatcctgg gattaaagcc tatatggaaa
    24361 atgtggtgcg tgaggctaaa gacaagggct atgtagagac cctctttaag cgtcgtcgtg
    24421 aattaccgga tatcaactct cgtaatttca acgttcgctc ttttgcggag cgtacagcta
    24481 tcaactcgcc cattcaagga agcgcagctg atattctaaa aattgctatg attaatctgg
    24541 ataaggcctt gcaagcaggt ggttttagag ctaaaatgct gcttcaagtg catgatgaaa
    24601 tcgttttgga agtgccaaat gatgagttga ctgctataaa aaaacttgtc aaagacacca
    24661 tggaagctgc tgttgattta gctgttcctt tatgtgtcga tgaaagtact gggcagagct
    24721 ggtacgaagc caaataagtt ttcttacttg ttatttctca ttttgacaac ttatgttata
    24781 ataaactaaa aaattgtagg agaagtcatg aagctgcttt gagctttttg acggataagg
    24841 ttaaaagtga ttgaagccat gctttaggag ttatcctgct atattgaaca gctgtagagg
    24901 ttattaaata gataggggaa caagtgattt attcgtttca aaatccctca gaggatgtcc
    24961 taaaagctta cttggagtca gccaaaacca ttgcggttgt tggtctttct gaccgcaaag
    25021 acacagcagc ttatggtgtc gccaaattta tgcaggctat ggattatcgg atcattccag
    25081 tcaatcccaa attagcaggt cagcttattt tgggagagaa ggtctatgcc agtatcaagg
    25141 caattccctt tgaggttgac attgtagatg tctttcgcag aagtgagttt ttaccagaag
    25201 ttgccagaga ctttttagca ggtcaggcca aagtcttttg ggcgcaatta ggccttgaaa
    25261 accaagaagc acaaaccatt ttacggtcgg caggcaaaga ggctattgtg atgaaccgct
    25321 gcctaaaaat agactatttg caattagtcg ctaaaccaga atgcatcacg aatcattgaa
    25381 actagagacg tgcgtgataa taaaggaaaa aaggatagga gtagcagctt atggacattc
    25441 attcacatca gcaagcccta gatgcctatg aaaatgtcct agagcatcta cgggaaaaac
    25501 atattcgaat tacagagaca cgtaaagcca ttatttctta catgattcag tccacagaac
    25561 accctagtgc cgataagatt taccgtgact tacagcctaa ctttcctaac atgagccttg
    25621 ccacagtcta taataatttg aaagttttag ttgatgaagg ttttgtctca gagttgaaaa
    25681 tcagcaatga tttaaccacc tactatgact ttatgggcca tcaacacgtc aatgtggttt
    25741 gtgaaatatg tgggaagatt gctgacttta tggatgttga tgttatggat attgccaaag
    25801 aagcccatga gcaaacaggt tataaggtca cgcgtatccc agttattgct tacgggattt
    25861 gtccggattg ccaagcaaaa gatcaacctg atttttagag ttaaccctta aataaaggca
    25921 ttataaatat agtgccttta caaatcaaaa acccccagtt cactatcaga cctttgttgc
    25981 ttatgttaaa gagttatttg ctggggatca taaggaatta aaggaaagaa ccatcaatca
    26041 tctcctctat tgtttgttcc tcacctggga aaatctcttt cttacaaatc aatcgcgtcg
    26101 tcgcttgaaa ctattagtgg ttgagagaag ttataacaat gttggcaact tcttaaaaga
    26161 gtattttgga ggattttttg agattgtcaa ctttgatgac ttgaggctga ccatggttga
    26221 tatggtgtca ctgtcttcag agtatgacgt aattgtgaca gatatgattc ttgagcaaac
    26281 aacggactcc gaaattttat ttttcaatca gatggcccct agtgtggtgg cgaatagatt
    26341 gacagacatg taactaaaca atgataatat ctgtgagagc atcggaattt aaaaaatgag
    26401 cgataaataa gattaagaat aagttagact tac
//
LOCUS       DAUCLR010000021        26151 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.021, whole genome shotgun sequence.
ACCESSION   DAUCLR010000021 DAUCLR010000000
VERSION     DAUCLR010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26151
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.021"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            49..1296
                     /locus_tag="VGT87_000678"
     CDS             49..1296
                     /locus_tag="VGT87_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEQ3813556.1"
                     /translation="MKSWQKVIVGGASLTLASTLLVGCGSGSKDKKEAGADSKTIKLW
                     VPTGSKKSYADTIAKFEKDSGYTVKVVESEDPKAQEKIKKDASTAADVFSLPHDQLGQ
                     LVESGTIQEVPEKYNKEIAATSTDQALVGAQYKGKTYAFPFGIESQVLFYNKSKLAAE
                     DVTSYDTITTKATFGGTFKQANTYATGPLFMSVGNTLFGENGEDVKGTNWGNEKGAAV
                     LKWIADQASNKGFVSLDANNVMSKFGDGSVASFESGPWDYEAAQKAIGKENLGVAIYP
                     KVTIGGETVQQKAFLGVKLYAVNQAPAKGDTKRIAASYKLASYLTNAESQENQFKTRN
                     IVPANKEVQSSEAVQSNELAKTVITMGSSSDYTVVMPKLSQMGTFWTESAAILSDAFN
                     GKIKESDYLTKLQQFDKDIAATK"
     gene            1570..2931
                     /locus_tag="VGT87_000679"
     CDS             1570..2931
                     /locus_tag="VGT87_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEQ3813557.1"
                     /translation="MTQSNLTPNISVIEALKRGSWDIKLSSIIMGFANFANKQFIKGI
                     LFLISELIFLVAFVSQIIPAIRGLVTLGTQTQGMTTKTIDGINIQVAVDGDNSMLMLI
                     FGLASLIFCLVFAYIYWCNLKSARNLYLFKQKGQKIPSFKEDLATLTNGRFHMTLMAI
                     PLIGVLLFTILPLIYMICLAFTNFDHNHLPPKSLFDWVGLANFGNVLSGRMAGTFFPI
                     FSWTLIWAVFATVTNFFFGIILALLINTKGLKWKKMWRTIFVITIAVPQFISLLIMRN
                     LLNDEGPLNALLNKIGLINGSLPFLSDPLWAKFSIIFVNMWIGIPFTMLIATGIIMNL
                     PSEQIEAAEIDGASKFQVFKSITFPQILLIMTPNLIQQFIGNINNFNVIYLLTGGGPT
                     NSEYYQAGTTDLLVTWLYKLTVTAADYNLASVIGILIFTVSAIFSLLAYTRTASYKEG
                     AAK"
     gene            2931..3767
                     /locus_tag="VGT87_000680"
     CDS             2931..3767
                     /locus_tag="VGT87_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEQ3813558.1"
                     /translation="MKNKRRFQLGLVYATLIILSIIWLFPIAWVILTSFRSEGTAYVN
                     YFIPKTFTLNHYINLFTNETFPFGKWFMNTLIVATFTCIISTFITVAIAYSLSRIKFK
                     FRNGFLKLALILNMFPGFMSMIAIYYILKALGLTQTLTALVLVYSSGAALGFYIAKGF
                     FDTIPYSLDESAMIDGATRMDIFFKITLPLAKPIIVYTALLAFMGPWIDFIFAQVILG
                     DATSKYTVAIGLFSMLQPDTINNWFMAFTAGSVLIAVPITLLFMFMQKYYVEGITGGS
                     VK"
     gene            3954..4770
                     /locus_tag="VGT87_000681"
                     /pseudo
     CDS             3954..4770
                     /locus_tag="VGT87_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001871253.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(5242..6225)
                     /locus_tag="VGT87_000682"
     CDS             complement(5242..6225)
                     /locus_tag="VGT87_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEQ3813559.1"
                     /translation="MVIIAVVIFFGVILGILIVEYGQKRSFSSWLVEKMFAYRKYKLS
                     KKVDNYKGVETQRNRNIPYHLDNPEEIVGFSTQEYDVNGMQVFIINDQKQSHQPVIYY
                     LYGSVYLRRPEKLHFKTVKHLIQLTNAKVVFPNFPKVPYATYQDVYPKLVEAYQQFCL
                     SNGPSQVSVIGDSSGGGMALAFAKYIRDTKTAPQPKQLILISPWLDVSFKNQAYKDFE
                     AEEAYLDARRLRDIGELWAGGQGNMQLPYVSPLYGDYEDLPEITTFVGTKEIFYPDIM
                     SLHDQLTRQRIANQLIIAEKMIHAYVIFPIREAKQARKQIAELVLKTVEHT"
     gene            complement(6256..7506)
                     /gene="dltD"
                     /locus_tag="VGT87_000683"
     CDS             complement(6256..7506)
                     /gene="dltD"
                     /locus_tag="VGT87_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEQ3813560.1"
                     /translation="MLKRLWLILGPLLIAFVLVVITIFSFPTQLDHSIAQEKANAVAI
                     TDSSFKNGLIKRQALSDETCRFVPFFGSSEWSRMDSMHPSVLAERYKRSYRPFLIGKR
                     GSASLSHYYGMQQITNEMQKKKAIFVVSPQWFTAQGINPSAVQMYLSNTQVIEFLLKA
                     RTDKESQFAAKRLLELNPGVSKSNLLKKVSKGKSLSRLDRAILKCQHQVALREESLFS
                     FLGKSTNYEKRILPRVKGLPKVFSYKQLNALATKRGQLATTNNRFGIKNTFYRKRIAP
                     KYNLYKNFQVNYSYLASPEYNDFQLLLSEFAKRKTDVLFVITPVNKAWADYTGLNQDK
                     YQAAVRKIKFQLKSQGFHRIADFSKDGGESYFMQDTIHLGWNGWLAFDKKVQPFLETK
                     QPVPNYKMNPYFYSKIWANRKDLQ"
     gene            complement(7499..7738)
                     /gene="dltC"
                     /locus_tag="VGT87_000684"
     CDS             complement(7499..7738)
                     /gene="dltC"
                     /locus_tag="VGT87_000684"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEQ3813561.1"
                     /translation="MSIEETVIELFDRLFMEDVSEMMDEDLFDAGVLDSLGTVELIVE
                     LESTFNIKVPISEFGRDDWNTVTKIVQGVEELQHA"
     gene            complement(7756..9012)
                     /gene="dltB"
                     /locus_tag="VGT87_000685"
     CDS             complement(7756..9012)
                     /gene="dltB"
                     /locus_tag="VGT87_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEQ3813562.1"
                     /translation="MMMFFSHIPYMEPYGNPIYFVYLILAFLPVIIGIFKQKRLSTYE
                     TLVSLVFILFMFGGDHYQQLVAFLFYLLWQIISVFAYQKYREKANSAGVFYLAIAMAL
                     FPLIWVKVVPLTGPSSQTLFSFLGISYLTFKSIGMIIEMRDGTLQEVRLPDFIRFMIF
                     FPTFSSGPIDRFRRFQEDYHKLPERDDYFAMLNKAVMYLMLGFLYKHIISYCLGGILL
                     PLLENKALMVGGYFNKETILVMYVYGLNLFFDFAGYSMFAIGISYLLGIRTPENFNMP
                     FLSASLKDFWNRWHMSLSFWFRDYVFMRLVHLLIKHKTFKNRNVTSGVAYLVNMLVMG
                     FWHGLTWYYIAYGLFHGIGLIINDAWIRKKKEINRHRKKKGLSPLFQSRAFHVLCIVV
                     TFHVVMFSLLLFSGFLNDLWFNRPLH"
     gene            complement(9009..10547)
                     /gene="dltA"
                     /locus_tag="VGT87_000686"
     CDS             complement(9009..10547)
                     /gene="dltA"
                     /locus_tag="VGT87_000686"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEQ3813563.1"
                     /translation="MIKDMIDSIEQFAQTQADFPVYDCLGERRTYGQLKRDSDSIAAF
                     IDSLALLAKSPVLVFGAQTYDMLATFVALTKSGHAYIPVDVHSAPERILAIIEIAKPS
                     LIIAIEEFPLTIEGISLVSLSEIESAKLAEMPYERTHSVKGDDNYYIIFTSGTTGQPK
                     GVQISHDNLLSFTNWMIEDAAFDVPKQPQMLAQPPYSFDLSVMYWAPTLALGGTLFAL
                     PKELVADFKQLFTTIAQLPVGIWTSTPSFADMAMLSDDFCQAKMPALTHFYFDGEELT
                     VSTARKLFERFPSAKIINAYGPTEATVALSAIEITREMVDNYTRLPIGYPKPDSPTYI
                     IDEDGKELASGEQGEIIVTGPAVSKGYLNNPEKTAEAFFTFKGQPAYHTGDIGSLTED
                     NILLYGGRLDFQIKYAGYRIELEDVSQQLNQSPMVASAVAVPRYNKEHKVQNLLAYIV
                     VKDGVKERFDRELELTKAIKASVKDHMMSYMMPSKFLYRDSLPLTPNGKIDIKTLINE
                     VNNR"
     gene            complement(10559..10702)
                     /locus_tag="VGT87_000687"
     CDS             complement(10559..10702)
                     /locus_tag="VGT87_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEQ3813564.1"
                     /translation="MIKNKSRMRGIAVFIGKTILFYLILMLLVYFFGYLGHGQSNFIY
                     NEF"
     gene            complement(10966..12957)
                     /gene="uvrB"
                     /locus_tag="VGT87_000688"
     CDS             complement(10966..12957)
                     /gene="uvrB"
                     /locus_tag="VGT87_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEQ3813565.1"
                     /translation="MIDKRDDKPFKLKSKYKPSGDQPQAIESLVDNIEGGEKAQILLG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPDNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVVASVSCIYGLGSPKEYA
                     DSAVSLRPGQEISRDTLLNQLVDIQFERNDIDFQRGCFRVRGDVVEVFPASRDEHAFR
                     VEFFGDEIDRICEIESLTGKTIGEVDHLVLFPATHFVTNDEHMEQSIAKIQAELAEQL
                     QLFESEGKLLEAQRLRQRTEYDIEMLREMGYTSGVENYSRHMDGRSPGEPPYTLLDFF
                     PEDFLIMIDESHMTMGQIKGMYNGDQARKQMLVDYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGEYEMSQTNTIIEQIIRPTGLLDPEIDVRPSMGQMDDLLGEINQRVARD
                     ERTFITTLTKKMAEDLTDYLKEMGVKVKYMHSDIKTLERTEIIRDLRLGVFDVLIGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNVDGHVIMYADKMTDSMQ
                     RAIDETARRREIQIAYNKAHGIVPQTIKKDIRGLISISKTSHNDISKEEMDYESMSRG
                     ERKEAINALQKQMQEAAELLDFELAAQMRDLILELKLMD"
     gene            13150..15324
                     /locus_tag="VGT87_000689"
     CDS             13150..15324
                     /locus_tag="VGT87_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEQ3813566.1"
                     /translation="MTHKIKVLLLAIMSIFLTCNIASAETIAIVSDTAYAPFEFKDSD
                     QIYKGIDVDIINEVAKRQSWDFSMSFPGFDAAVNAVQSGQASALMAGTTITNARKKVF
                     HFSEPYYDTKIVIATRKANAIKKYSDLKGKTVGVKNGTAAQAFLNNYKKKYDYTVKTF
                     DTGDLMYNSLSAGSIAAVMDDEAVIQYAISQNQDIAINMKGEPIGSFGFAVKKGSGYD
                     YLVNDFNTALKAMKADGTYQAIMTKWLGTDDKATTSQATGNPSAKATPIKDSYKIVSD
                     SSFAPFEFQNGKGKYVGIDIELIKAIAKQQGFKIEIANPGFDAALNAVQSSQADGVIA
                     GATITDARKAIFDFSDPYYTSNIILAVKAGKNIKNYEDLDRKTVGAKNGTSSYSWLKE
                     NAPKYGYNVKAFDDGSSMYDSLNSGSVDAIMDDEAVLKYAISQGRRFETPLEGISTGE
                     VGFAVKKGTNPELIEMFNNGLAALKKSGQYDDIIDKYLDSKKAATPSEKGADESTISG
                     LLSNNYKQLLAGLGTTLSLTLISFAIAIIIGIIFGMMAVSPTKSLRLISTVFVDVVRG
                     IPLMIVAAFIFWGVPNLIESMTGHQSPINDFLAATIALSLNGGAYIAEIVRGGIEAVP
                     AGQMEASRSLGLSYGTTMRKVILPQAVKLMLPNFINQFVISLKDTTIVSAIGLVELFQ
                     TGKIIIARNYQSFRMYAILAIIYLIMITLLTRLAKRLEKRLN"
     gene            15324..16064
                     /locus_tag="VGT87_000690"
     CDS             15324..16064
                     /locus_tag="VGT87_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003107174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEQ3813567.1"
                     /translation="MTELKIDVQDLHKSYGQNEVLKGIDAKFYEGDVVCIIGPSGSGK
                     STFLRTLNLLETITSGKVMVDGFELSDPKTNIDKARENIGMVFQHFNLFPHMTVLENI
                     IFAPVELGKESKEVAKKHGMALLEKVGLSDKADAFPGSLSGGQKQRVAIARSLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKDLAEQGMTMLIVTHEMGFARQVANRVIFTDGGQF
                     LEDGTPEEIFDHPKHPRLIEFLDKVLNV"
     gene            complement(16212..16364)
                     /locus_tag="VGT87_000691"
     CDS             complement(16212..16364)
                     /locus_tag="VGT87_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813568.1"
                     /translation="MTEKNGATFLMAVGFFLIAISPMVGNKYLHIVTGLALTGLGYYL
                     TKHKRP"
     gene            complement(16361..17740)
                     /locus_tag="VGT87_000692"
     CDS             complement(16361..17740)
                     /locus_tag="VGT87_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEQ3813569.1"
                     /translation="MSHFSDKFMEISGKIGSQRHLVAIRDSFISMMPITMAGSVAVLL
                     NVFLRDIPNNMGWTGFAKAMQPVIDINGYVYFGTIGIMALFFAFAFGYNLAVMHKINP
                     LAGGLISFGSFIATLPQTLTISTPLENASANLISNLKEMGLSVVTAGGASSIETSQWG
                     AIALKYVGATGLFTALIIGFLSSFVYAALTKRNITITLPDNVPPAVNKAFAAIIPGTV
                     AIYASAIFAYLIFALTGSSLSDVISTYIQLPLLGLSQGIGSVILLTFLVQLLWFFGLH
                     GHNVLAPVMDGIYMVALTENTAAYNTAHSAANLPYLWTRGSFDAYAQMGGSGVTLALI
                     IAIFIFSKREEHKTIAKLSAPMGVFNINEPITFGMPIVLNPTFVIPWLIVPPICASIA
                     YFATAIGLIPPVFLSVPWITPVGLYAYLATGGNIMAGLVSLFNLFVAFLIWAPFVILA
                     NKEKASDLA"
     gene            complement(17918..18367)
                     /locus_tag="VGT87_000693"
     CDS             complement(17918..18367)
                     /locus_tag="VGT87_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HEQ3813570.1"
                     /translation="MIISAEGDVSIHHCFEAMLISLQEDYRQNTLRQLSRDDIKAGIN
                     YVKHYGKYKERTVRVLITALEAPQLYTACFSSNHGIKELAYQLEALGENRTRITYSYD
                     YHPLDIFQKANQFIVGKLFKKSLERQSQAQLAALIQYAKQLQQPSSL"
     gene            complement(18364..18699)
                     /locus_tag="VGT87_000694"
     CDS             complement(18364..18699)
                     /locus_tag="VGT87_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEQ3813571.1"
                     /translation="MTEPSNLEAIMGLIMYGGEAKGKAVEAIHAARDGQFEEAQHLLQ
                     EATNSLNIAHKSQTHLLSQEAAGDAVELSLLMVHGQDHMMTALAFIDLAKELVTVYDK
                     MSAIQRGES"
     gene            complement(18702..19013)
                     /locus_tag="VGT87_000695"
     CDS             complement(18702..19013)
                     /locus_tag="VGT87_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEQ3813572.1"
                     /translation="MKSIMLVCNAGMSTSMLVTKMQKAAQARDLEVSIWAVPVSEADN
                     EVAANSIDVLLLGPQVKFLLKDFKDKFEPDIKVDAINMADYGLMNGEKVLETALAMME
                     E"
     gene            complement(19036..21030)
                     /locus_tag="VGT87_000696"
     CDS             complement(19036..21030)
                     /locus_tag="VGT87_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEQ3813573.1"
                     /translation="MLSKKELAIITFLIHHKEQFVSSATLAEVIGMSDRTVRKYLKEL
                     ISSLPEHGAHIISKQGRGYCLEIDHSMAFDIFWQESVTSKKRLADVSQVEESVDREDY
                     VLHKFFFEDAVQDFEELCQELYISRTTLKHVLAGIKERIIPYQLELEITHQHIQIRGK
                     EEDIRHFIMDYFFVTSFDNTLSTMVGNIFLEGINFAEIMIIVLDECRDAKLKLSDFVI
                     NNLVLHIALMVQRIRSGCPLELFSIPIAIRQSDEYQVALRILYRVEEVMGIRFPKEEA
                     NYIALHLKVKHSVGKHWQDDNTDEKLQDHLKACIAKISQLTGMTLETDTNLFQGLLAH
                     MMPLITRLENHIQLTNPLTEEIKSQYPEIFTLTKQTFSDLLVCQKNDVSDDEWAYISL
                     HLMAAIERYSNRHKLRVLVVCATGYGSAMMLKNRLEKEFEGRLRIVDVISYYEITEER
                     LKTVDLIISSISLANLMFLTPVITVSVFLSNQDIETIRQFIGEQEGIKKEVSLPSQMS
                     LSKAEQLLTGVFSPNRFLYLDEKISKEDLLLRMIACLDEAGTETFVEDFYHQMVLREN
                     YSPVIYGEVLAFPHPANPMTYSEQVVVSICREPLEWDKAHQAVHFVFLLSPSKGHNHR
                     LKYVSPGLASFVNQVELQQALLEEPNYSKFMTVFTPLIHD"
     gene            complement(21136..22230)
                     /locus_tag="VGT87_000697"
     CDS             complement(21136..22230)
                     /locus_tag="VGT87_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEQ3813574.1"
                     /translation="MVDLGFSLYPERYDVTKSKAYIDLCHSYGAKRLFMSLLQLAPAD
                     HQMFHCYAELIAYANQLGIRVIADVSPSFISQAGWSDQLIERAHAFGLAGLRLDEALP
                     LAEIVTLTRNPFGLKIELNMSTDKQLLMSLLATDAERSNIIGCHNFYPHEFTGLSWQH
                     FKDMSRFYHEHDIETAAFITAQSASEGPWLLAEGLPTVEDHRHLPIGLQVELMKAIGT
                     IDNILISNQFISEEELAACTQALARPVTTIKVRPIIDLTEVEEQIIGYPHCYRGDVSD
                     YVIRSTMPRLVYAQESIAPRDQSKEVKRGSIIIDNDRYHRYKGELQIALKNFTVSSKA
                     NVVAEVREDYLSLLDDLRPWQEFCLEIDPS"
     gene            complement(22239..23639)
                     /locus_tag="VGT87_000698"
     CDS             complement(22239..23639)
                     /locus_tag="VGT87_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEQ3813575.1"
                     /translation="MKQRKHRYQFPDGFLWGSSTSGPQSEGTVPGDGKGPSNWDYWFS
                     IESAKFHHQIGPEKTSTFYENYKGDIALLKETGHTIFRTSIQWSRLIPEGVGEVNPKA
                     VTFYREVFQDIIAQGIKLIVNLYHFDLPYALQEKGGWENKATVWAYETYAKTCFELFG
                     DLVNTWITFNEPIVPVECGYLGYYHYPCKVDAKAAVQVAYNTQLASSLAVKACHKLHP
                     DHKISIVLNMTPAYPRSNAPEDVKAARIAELFQTKSFLDPSVLGVYPAELVSILEEAD
                     LLPQYSADELEIIKNNTVDFLGVNYYQPLRVQAPSKSQQEGDPLILDIYFEPYDMPGK
                     KVNPHRGWEIYEPGLYDIALNLKEHYGNIEWLVTENGMGVEGEEAFLADGQIQDDYRI
                     TFIEDHLIQLHKALGEGANCKGYLLWTFIDCWSWLNAYKNRYGLVALDLESQKRTIKK
                     SGYWFKALSESNGFDK"
     gene            23864..24559
                     /locus_tag="VGT87_000699"
     CDS             23864..24559
                     /locus_tag="VGT87_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEQ3813576.1"
                     /translation="MAYLNYFTKTEWALWLSSVLAILISALLFGKQAPLALIASLIGV
                     TSLIFSAKANPIGQGLVIIFSIIYAYLSLRNSYYGELMTYLFMTLPMTIFSLFTWLNH
                     PFEGKKSQVTISRLTPTDRRCLFVFTILVTLIFYGILASIQTAYLLVSTLSIATSFSA
                     VYLSYKRSPYFALAYGLNDLVLILLWMHAAQTDSSQYAVVICFATFLINDSYTFYSWL
                     MLQRYQEKKVKKG"
     gene            complement(24564..24722)
                     /locus_tag="VGT87_000700"
     CDS             complement(24564..24722)
                     /locus_tag="VGT87_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813577.1"
                     /translation="MGDKPISFKDKDGNFVSAADVWNAEKLEELFNLLNPNRRLRLER
                     EKLKKDEA"
     gene            complement(24791..26104)
                     /gene="obgE"
                     /locus_tag="VGT87_000701"
     CDS             complement(24791..26104)
                     /gene="obgE"
                     /locus_tag="VGT87_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEQ3813578.1"
                     /translation="MSMFLDTAKISVQAGRGGDGMVAFRREKYVPNGGPWGGDGGKGG
                     SVIFRVDEGLRTLMDFRYNRKFKAKSGEKGMTKGMHGRGAEDLIVFVPQGTTVRDAET
                     GKVITDLVEHGQEVVIAKGGRGGRGNIRFATPRNPAPEIAENGEPGEERQLELELKIL
                     ADVGLVGFPSVGKSTLLSVVSSAKPKIGAYHFTTIVPNLGMVRTKSGDSFAMADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSASEGRDPYEDYVSINNELETYNLRLME
                     RPQIIVANKMDMPEAQENLKAFKKKLATQYDEFNDLPMIFPISSLAHQGLENLLEATA
                     ELLAKTDEFLLYDEADLVDEEAYYGFAETEKDFEITRDDDATWVLSGEKLERLFVMTN
                     MERDESIMKFARQLRGMGVDEALRERGAKDGDPVRIGKFEFEFVD"
BASE COUNT         8112 a         5435 c         4593 g         8011 t
ORIGIN      
        1 ggttgcataa tattaagtgt aagaaatata ccttaggagg aaatagctat gaaatcatgg
       61 caaaaagtta tcgtcggcgg agcaagtttg acacttgcaa gtaccttgtt agtgggatgt
      121 ggatcaggtt caaaagataa aaaggaagct ggcgctgact caaagactat taaactttgg
      181 gtaccgactg gctctaaaaa atcatatgca gataccattg caaaatttga aaaagattct
      241 ggttacaccg ttaaagtggt tgaatcagaa gatccaaaag cacaagaaaa aatcaaaaaa
      301 gatgcctcaa ctgctgccga tgtcttttca cttcctcatg atcaacttgg tcaacttgta
      361 gaatctggta ctattcaaga agttccagaa aagtacaata aagaaattgc tgctacttct
      421 actgatcagg cgctcgtcgg tgcacagtac aaagggaaaa catatgcttt cccatttggt
      481 attgaatcac aagttctttt ctacaataaa tcaaaattag ctgctgaaga cgttacttca
      541 tatgatacaa ttactacaaa agcaactttt ggtggtacct ttaaacaagc taacacttac
      601 gctactggtc cattattcat gtcagttggt aacactttat ttggggaaaa cggtgaagat
      661 gtaaaaggta ctaactgggg caatgaaaaa ggcgctgctg ttcttaaatg gatcgcagac
      721 caagcttcaa ataaaggttt tgttagctta gatgctaaca acgttatgtc taaatttggt
      781 gacggttcag tagcttcttt tgaatcagga ccgtgggact atgaagctgc tcaaaaagct
      841 atcggtaaag aaaaccttgg tgttgctatt tatccaaaag taactattgg aggcgaaacg
      901 gttcaacaaa aagcattctt gggtgtaaaa ctttacgcag tcaaccaagc accagctaaa
      961 ggtgatacaa aacgtatcgc agctagctac aaattggcat cctacttgac taatgctgaa
     1021 agccaagaaa accaattcaa gactcgtaac attgtcccag ccaacaaaga agttcaatct
     1081 tcagaagctg ttcaatcaaa cgaacttgct aaaaccgtta tcactatggg ttcttcttca
     1141 gactacactg tcgtaatgcc aaaacttagc caaatgggca cattctggac tgaaagtgct
     1201 gctattctta gtgatgcctt caacggtaaa atcaaagaaa gcgactacct tactaagttg
     1261 caacaattcg acaaagatat cgctgcaaca aaataatatg atctcatgtc atcactgcta
     1321 tactaaaaca agcacaaggt aagtacttct aaaaagttct ttccacttac cttatgaaga
     1381 gtggaactag gctccatcac attttattca agcttagtct cactcttact tgtttttgtt
     1441 ttactagtag cttagtgcta cttaaatttt taactcttta aaagtttacg taacacttag
     1501 ctatttcatc ctaacaagag attgaatctg aactaccact ctagtatcct aaaataaagg
     1561 agaatctata tgactcagtc aaacttgaca ccaaatatat ctgtaataga agctttaaaa
     1621 agaggcagtt gggatattaa actttcgagt attatcatgg gatttgccaa ctttgctaac
     1681 aaacagttta tcaaaggaat cttattttta ataagtgaac ttatctttct agtagctttt
     1741 gtctcccaaa tcatccctgc tatccgtggc cttgtcactc ttggtactca aactcaggga
     1801 atgactacaa aaactattga tgggattaac atccaagttg ctgttgatgg cgataactct
     1861 atgttaatgc ttatttttgg tttagcatca ctcattttct gcctagtctt tgcttacatt
     1921 tactggtgta atctcaaaag tgctcgtaac ctttatcttt tcaaacaaaa aggtcagaag
     1981 ataccaagct tcaaagaaga tcttgccaca cttacaaatg gtcgctttca tatgacttta
     2041 atggctatcc cactgattgg tgtgctttta ttcaccattc ttcctttgat ttacatgatc
     2101 tgtttggcct ttaccaattt tgatcataat cacttgcctc caaaatcttt atttgattgg
     2161 gtgggacttg ctaactttgg aaatgtttta agcggccgta tggcaggaac cttttttcct
     2221 atcttctcat ggactttgat ttgggctgtt tttgccacag taactaattt cttctttggt
     2281 attattcttg ctttgttaat caataccaaa ggattaaagt ggaaaaaaat gtggcgaacc
     2341 atctttgtta tcaccattgc agttccacaa ttcatctcat tattgattat gcggaactta
     2401 ctcaatgacg aaggcccact gaatgctctt ctcaataaaa tcggcttgat taatggctca
     2461 ctgccatttt tatctgatcc tctttgggca aaattctcaa tcatttttgt caacatgtgg
     2521 atcggtattc catttaccat gttgattgcg actggtatta tcatgaacct tccaagtgaa
     2581 caaattgagg ctgctgaaat tgatggcgct agcaaattcc aagtcttcaa gtctatcaca
     2641 ttccctcaaa ttctcttgat catgacacca aacttgattc aacaatttat cggaaatatc
     2701 aataacttta acgtcattta cctccttact ggtggtggtc caacaaattc agaatactat
     2761 caggcaggaa caacagactt actggtcact tggctctata aattaaccgt cacagctgct
     2821 gactacaatt tagcttctgt tatcggtatc ttaatcttta cagtttcagc tatctttagc
     2881 ttacttgctt atacaaggac agcatcctac aaggaaggag cggctaaata atgaaaaaca
     2941 aacgtcgatt tcaacttgga cttgtttatg ctactttaat catcctctca atcatttggc
     3001 tcttcccaat cgcttgggtt attcttacta gcttccgtag cgaaggaact gcttacgtca
     3061 attattttat tcctaagact tttacattaa atcactacat taatcttttt actaatgaaa
     3121 cctttccttt tggaaaatgg tttatgaata ctcttattgt agccactttt acatgtatta
     3181 tttcaacctt cataactgtt gccatagcct actctcttag ccgaattaag tttaagtttc
     3241 gcaatggctt tttaaaacta gcccttattt taaatatgtt ccctggtttc atgagcatga
     3301 ttgccatcta ctatatctta aaggccttag gtttgacgca aaccctaaca gcacttgtcc
     3361 ttgtttattc ctcaggtgca gcccttggtt tttacattgc aaaaggattt tttgatacca
     3421 ttccttattc acttgatgaa tcagctatga ttgatggcgc tactcgtatg gatattttct
     3481 ttaaaataac ccttccttta gcaaaaccaa tcattgtcta tactgccctt cttgctttta
     3541 tggggccttg gattgacttt atctttgcac aagttatttt aggtgatgct acaagtaaat
     3601 ataccgttgc tattggacta ttttcaatgc ttcagccaga taccattaac aactggttta
     3661 tggccttcac tgctggttct gtcttaattg ccgtcccaat cactctcctc ttcatgttta
     3721 tgcaaaaata ctatgttgaa ggcatcacag gtggttctgt taaataagtt acaacatacc
     3781 aaaccttcta agggcaagcc ctttaggagg tttttgataa ataaggccgt agataatcat
     3841 attagtcctc acatttatct tcagatcccg cgttgtaaaa tgaagtgcaa caaaaaagac
     3901 atgttcgtct gatatactag aattccccaa ttcagtatgg aaagcagatg aacatgtcta
     3961 atattaattc taccagaaaa tcctcctatt ctcatctttc agccacagaa cggggtgaaa
     4021 ttgctgctta tcttaaaatg ggaaagaaac ccgttgagat tgctcgactt ctgggtagtc
     4081 accgttctac aatctgccga gaaataaaac gtggttcagt agatcaggta aaggataaga
     4141 atggaaaaca aaccttcttc aacgcctatt tcgctgatag tgggcaacgt gtctattaaa
     4201 ccaatcgatt tgcctaagat ggttagtatc agaaaacgtt ctaagaaagt catgaaaacg
     4261 aataagaaga ctttaggtaa atctatcgaa gaacgtccag aatatattaa tgatcgttct
     4321 gaatttgggc attgggagat tgatttgatt ctcggcaaga aaaccaaagg tgaagctgtt
     4381 atgttgactt tggtagagcg ccagacacgc tatgcactag gagttaagct agaagacaag
     4441 cagtcccaaa ccattaaccg cgctgtcacg catctcatca gtcagtatcc tattgcatcc
     4501 atcacagcag ataatggttc tgagtttagt ttactctcaa ccttagaagc tgttgacgtt
     4561 tactttgcac atccctattc ttcacatgag agaggaacaa acgagaactt caatggcctc
     4621 ctcagagaat atgtccctaa aggaatctca cttaatccac taacctctga agaacttgac
     4681 aattatctta cagccatcaa tgagcgccca agacgacttc ttcaatatca atcctcaaca
     4741 ttcctgtttg agctagcccg aacagcttaa cctctggaac tctagttatc tatgaactcg
     4801 ttgcacttga cttgacaact tgcgagagca tatcagtagt ttttctgcta attgaaaaca
     4861 gggtatttac tgtcacacaa gtctgtcctt tctttttttc tacaacttta tttcgtcagc
     4921 gtaaataaaa caaaagctct ataatctctt gaatgggtaa atccactgta gagattatag
     4981 agctttttga atatcaaaaa agtcccataa gatttataat gaaaagcaac caaactctca
     5041 ttagaaagac tcttatggaa catatcaatc ataccttaca acacatcgga attaaagaca
     5101 aaaatatcac tctaaacaat gttttgaagg atgaatctcc tactgagctc gttactactt
     5161 atcagaaagg tagataggaa atcctgatga agagtgtgtc caagggatta tcaattaggt
     5221 cttagattac aaaatagtaa ttcaggtatg ctctactgtt ttcaacacta gttctgcaat
     5281 ttgttttcga gcttgcttag cttctcttat tggaaaaata acatacgcat gaatcatttt
     5341 ttctgcaata atcagctgat tagcaattcg ttgtcttgtt aattgatcat gtaaagacat
     5401 aatgtctggg taaaaaatct cttttgtccc aacaaaggtc gttatttcag gcaaatcctc
     5461 ataatcacca tacaaaggac tgacataagg aagttgcata ttaccttgac cacctgccca
     5521 tagttcacct atatcacgaa gacgacgagc atctaggtaa gcttcttctg cttcaaaatc
     5581 cttgtaggct tgatttttaa aactaacatc taaccaagga gatataagaa ttaattgctt
     5641 tggctggggt gctgttttag tatctcgtat atacttagca aacgccaaag ccataccccc
     5701 accagaagaa tcaccaatga cactaacttg actcggtcca ttagacaagc aaaattgctg
     5761 gtaagcttca actaatttag gatagacatc ttgataagta gcatacggta ctttaggaaa
     5821 gttaggaaaa acaaccttgg cattagtcag ctgtataaga tgcttaaccg tcttaaaatg
     5881 aagtttttcg ggtcgtcgta aatagacact gccataaaga taataaataa caggctgatg
     5941 actttgcttt tgatcattaa taataaaaac ttgcatcccg ttcacatcgt attcttgagt
     6001 tgaaaaacct actatctctt caggattatc tagatgataa gggatattac gattacgctg
     6061 cgtctcaacc cccttataat tatcaacttt tttagataac ttatactttc tataagcaaa
     6121 cattttctca accaaccaac ttgaaaagga gcgcttttga ccatattcaa ctatcaaaat
     6181 acctagtatc actccaaaaa aaatcaccac agcaataatg accacttatt atcctcgttt
     6241 tctaatatta ttttactatt gcaagtcttt cctatttgcc caaattttac tataaaaata
     6301 agggttcatt ttatagttgg gcactggctg cttcgtttct agaaatggtt gcactttctt
     6361 atcaaaagct aaccagccat tccaaccgag atggatggta tcttgcataa agtaggactc
     6421 accaccatct tttgagaagt cagcaatgcg atgaaatcct tgtgacttta actggaattt
     6481 tattttacga actgccgctt gatacttatc ttgatttaag ccggtataat ccgcccaagc
     6541 tttattaaca ggagttataa caaagagtac atctgttttt cgtttagcaa attctgataa
     6601 taaaagctga aaatcattgt attctggtga cgccaggtaa ctataattaa cttggaaatt
     6661 cttataaaga ttgtatttag gtgctattcg tttacgataa aatgtatttt taatcccaaa
     6721 acggttgttg gttgttgcta attggcctct cttagttgct aatgcattca attgtttata
     6781 cgaaaatact ttaggtaatc ccttaacgcg aggcaaaatt cttttttcat agttagtaga
     6841 tttgcctaaa aaactaaaaa gggactcttc tctcaatgct acttgatgtt gacatttcaa
     6901 aatagctctg tctaaccgac taagagactt acccttactt acttttttca ataagtttga
     6961 tttagacaca ccagggttaa gctcaagcaa acgctttgct gcaaactgtg attctttatc
     7021 agttctagct tttagtaaaa attcaatcac ttgagtgtta gacaagtaca tctgaaccgc
     7081 actaggatta atcccttgag cagtaaacca ttgaggagat actacaaaga tggctttttt
     7141 cttttgcatt tcattggtaa tttgttgcat accataataa tgcgacaaag atgctgatcc
     7201 tctcttacca attaaaaatg gtctatagct ccgcttgtag cgctctgcaa gcaccgaagg
     7261 gtgcatacta tccattcgac tccattcgct agaaccaaaa aaaggcacaa aacgacaagt
     7321 ctcatctgat aaagcttgtc ttttaatcaa accattttta aaagaactat ctgtgatcgc
     7381 aacggcattt gctttttcct gagctatgga atgatcaagt tgtgtaggaa aactaaaaat
     7441 agtaatcact actaaaacaa aggcaataag aagaggacct agaattaacc agagtctctt
     7501 aagcatgttg caattcctct acaccttgaa caatcttagt tactgtgttc caatcatcac
     7561 gaccaaattc tgaaataggt actttaatat tgaacgttga ctctaactca acaatcaatt
     7621 cgactgtccc aagactatcc aacacacctg catcaaacaa gtcttcgtcc atcatctctg
     7681 aaacatcttc cataaacaaa cggtcaaaca attcaattac agtttcttca atagacattt
     7741 ttatacttcc ttttattaat gtagtggtct gttaaaccaa aggtcattta aaaaaccaga
     7801 gaataacaga agtgaaaaca tgacaacgtg gaaagttacc acgatacata agacatggaa
     7861 tgccctgctt tgaaagaggg gtgataagcc ttttttctta cggtgacggt tgatttcttt
     7921 tttcttacga atccaagcat cattaataat aagtcctata ccatggaaaa gcccataagc
     7981 gatataatac caggttaagc catgccaaaa tcccatgact agcatattaa ccagataggc
     8041 aacacctgaa gtgacatttc ggtttttaaa ggttttatgc ttaatcaata gatgaaccaa
     8101 tcgcataaac acataatctc taaaccagaa ggacaaactc atgtgccatc tattccaaaa
     8161 atctttcaaa cttgcagata agaaaggcat gttaaagttt tctggtgttc gaatacccaa
     8221 taggtaggaa ataccaatcg caaacatcga ataaccagca aagtcaaaaa agagattcaa
     8281 gccatacacg tacataacca gaatagtttc cttattaaag tagccaccaa ccatcaaagc
     8341 cttattttca aggagaggaa gtaagatgcc acccaaacaa taggaaatga tatgcttgta
     8401 tagaaaacct aacatgagat acatgactgc cttattgagc attgcaaaat aatcatcacg
     8461 ttcaggaagt ttatgataat cttcctgaaa acgtctgaag cgatcaatgg ggccacttga
     8521 gaaggtgggg aagaaaatca tgaagcgaat aaaatccggt aaacgaacct cttggagagt
     8581 cccgtcacgc atctcaataa tcattccaat acttttaaag gtcaaatatg aaatccctag
     8641 aaaactaaag agtgtttgag aagaaggacc tgttagggga acaactttga cccagataag
     8701 ggggaataag gccatcgcaa tagctaagta aaacactcct gcactattag ctttctcacg
     8761 gtatttctgg taagcaaaaa cactgatgat ttgccacaaa agataaaaga gaaaagcaac
     8821 taattgttga taatgatctc ctccaaacat aaagagaata aataccaaac tcaccaaggt
     8881 ttcataagtg cttaagcgct tttgtttaaa aatcccaatg atgactggta agaaagccaa
     8941 aataagatag acaaagtaaa tggggttacc gtaaggttcc atgtagggaa tgtggctaaa
     9001 aaacatcatc atcggttatt gacctcatta atcaaagttt taatatctat cttaccattt
     9061 ggagttaatg gtagactatc tcgataaaga aacttagaag gcatcatata actcatcata
     9121 tgatccttaa cagaggcttt gatagctttg gttaactcca attccctatc aaaacgctct
     9181 ttaaccccat ctttgacaac gatataggcc aagagatttt gaaccttatg ttctttattg
     9241 taccttggaa cagccacagc cgatgctacc attggtgatt gattcaactg ttgggaaaca
     9301 tcttctagtt caatacgata accagcatat ttaatctgaa aatccaaacg cccaccatac
     9361 agcaaaatat tatcttctgt cagtgagcca atgtcccctg tatgataagc aggctgtcct
     9421 ttaaaagtaa agaaagcttc tgcggttttc tcaggattgt tgaggtaacc ttttgacaca
     9481 gcaggccctg taacaataat ttcaccctgc tcccctgaag ctaactcctt accatcttca
     9541 tcaataatat aggtcggaga atctggtttg gggtagccaa tcggcaagcg ggtataatta
     9601 tccaccattt ctctggtaat ttcaatagcg gataaggcta cagtcgcttc tgttggtcca
     9661 taggcattga tgatcttggc actcgggaaa cgttcaaaga gttttcgagc cgtagaaacg
     9721 gttaattctt cgccatcaaa ataaaaatgc gttaaagctg gcatcttagc ctggcaaaaa
     9781 tcatcgctaa gcatggccat atcagcaaat gaaggagtcg atgtccaaat gccaactgga
     9841 agttgcgcaa tcgttgtaaa taactgttta aagtctgcca ccaattcttt tggaagtgca
     9901 aataaggttc cacctaaagc caaggtaggt gcccagtaca tgacagaaag gtcaaaggaa
     9961 tagggtggct gcgccagcat ttgtggctgt ttaggtacat caaatgctgc atcttcgatc
    10021 atccagttgg taaagctcaa aagattgtca tgtgaaattt gcaccccttt tggttggccc
    10081 gttgttccag aggtgaaaat aatgtaataa ttatcatctc ctttaacaga atgtgttcgc
    10141 tcatagggca tttctgctaa ttttgctgac tcaatctctg atagtgagac aagggaaatc
    10201 ccttcaatag taagagggaa ttcctcaata gcaatgatta aactgggctt tgcaatctca
    10261 ataatcgcta aaatacgttc tggtgctgaa tgcacatcta ctgggatata ggcatgacca
    10321 gatttggtta aagctacaaa agtagctaac atatcataag tttgcgcccc aaaaaccaaa
    10381 actggagatt ttgccagtaa agctaagcta tctataaatg cagcaatgct atcagaatct
    10441 cttttgagtt gtccgtaagt gcggcgttcc cctaaacaat cataaactgg gaaatcagcc
    10501 tgtgtctgag caaactgctc aatactgtca atcatatcct taatcatcag tcaatctcct
    10561 aaaattcatt ataaataaag ttactttgac catgtcctag ataaccaaag aaatagacca
    10621 gcaacattaa aatcagataa aataaaattg tcttgccaat aaaaacagca attcctctca
    10681 ttcttgattt gttttttatc atactttacc ttcttgtatt agtgaaataa tgtcctaaaa
    10741 aattctactc gaaaaaacct aacttgacaa gctaaaagtc atattattaa aaagaaaatt
    10801 tttttaaagc ttaactattt agacagttaa attttatgcc tttttactaa acttactaag
    10861 ttaatcttta ctttaaaaag ttaacttaaa attgtcctaa taaaaagaat tatttgactt
    10921 taacttatat atgttaataa gtataatata agctttattc aaaacctagt ccattaattt
    10981 aagttctaaa atcaagtctc gcatttgagc tgccaattcg aaatcaagta attcagctgc
    11041 ctcttgcatt tgtttttgca gagcattgat cgcttctttt cgttctcctc ttgacatact
    11101 ttcataatcc atctcttctt ttgatatatc attgtggctt gttttactta tactaataag
    11161 gcctctgatg tcttttttta tagtttgtgg aacgatgccg tgcgctttgt tatatgctat
    11221 ttgaatctca cgacgacgag ccgtttcgtc aatagcccgc tgcatagaat ctgtcatctt
    11281 gtccgcatac ataatgacat gaccatctac gtttcgagct gctctaccga tggtctgaat
    11341 caagccgcgt tcattacgga ggaaaccctc tttatcagca tccaaaatgg ctacaagact
    11401 aacttctggc acatcaatcc cttcacggag cagattgatt ccaatcaaga catcaaaaac
    11461 accaagtcgt aaatcacgaa taatttctgt ccgctctaag gttttaatat cactgtgcat
    11521 atacttgacc ttgacaccca tctcttttag ataatcagtc aaatcttctg ccattttttt
    11581 agttaaggtt gtgatgaacg tccgttcatc gcgcgctacg cgctggttaa tttctcccaa
    11641 caaatcatcc atttgcccca tacttggcct tacatcaatt tcaggatcaa gtagcccagt
    11701 cggacgaata atttgctcaa taattgtatt agtctgtgac atttcgtatt caccaggcgt
    11761 tgctgaaaca tagacaattt ggtgcacatg actttcaaat tcctcgcgtc ttaagggacg
    11821 attatcaaga gctgatggca atcgaaaacc atagtcaact agcatttgtt ttctggcttg
    11881 gtcaccatta tacatgcctt tgatttgtcc cattgtcatg tgactttcat caatcataat
    11941 taaaaaatct tctgggaaga aatctaacaa ggtataagga ggttctcctg gtgacctgcc
    12001 atccatatga cgggaatagt tttcgactcc gctagtatag cccatttcac gtaacatttc
    12061 aatatcatat tccgttcttt ggcgtaatcg ctgtgcttct agtagtttac cttcagattc
    12121 aaataactgt aactgctctg ctaactctgc ttgaattttg gctattgatt gctccatatg
    12181 ctcatcattt gttacaaagt gagtagctgg aaataaaacc aaatggtcaa cttctccaat
    12241 agttttccca gttaaacttt cgatttcaca tatacgatca atctcatccc cgaaaaattc
    12301 cacccgaaaa gcatgttcat ccctggatgc tggaaagacc tcaacaacat cgccacgtac
    12361 acgaaaacag ccgcgttgaa aatcaatatc attacgctca aattgaatat ctactaattg
    12421 atttagcaaa gtgtcacgtg atatctcctg acctggacgt aggctgactg ctgaatctgc
    12481 gtactctttt ggcgaaccta gtccataaat acaagataca gaagctacca ctatgacatc
    12541 gttacgctca agtaaagaag aggtggctga atggcgaagt ttatcgattt catcattgac
    12601 tgaactatct ttttcgatat aggtgtcgct tgatgggaca tatgcttcag gttggtagta
    12661 atcataatag gaaacaaagt attccacagc attgtctgga aaaaattctt taaattcacc
    12721 atataattgt cctgctaagg ttttattgtg agctatcact aaggtaggtt tatttacttt
    12781 actaattact tggctcatgg tgtatgtttt tcccgttcct gtagcaccta ggagaatctg
    12841 agctttttct ccgccctcga tattatcaac taggctttca atagcttgtg gctgatcacc
    12901 agacggttta tactttgatt ttagtttaaa tggtttatca tcacgtttat caatcatttt
    12961 ataccctcta atagtcttac tctcttatag actattttaa catagataag ccagaatcac
    13021 ctccaataag cgtaaaaaac atgtcattta catgttatgt tttatgacat ataaataaca
    13081 aatttcttgc ttaatgttca atattttgtt ataattaacc atatttttta gttttataag
    13141 gagattagta tgacgcacaa aataaaagta ttgctgcttg cgataatgtc tatttttttg
    13201 acatgcaata ttgcaagtgc tgaaactatt gctattgttt cagatacagc ttatgcccca
    13261 tttgaattta aagactcaga tcaaatttac aaaggaattg acgttgatat tattaatgaa
    13321 gtagccaaac gtcaatcttg ggatttcagt atgagtttcc cgggttttga tgcagctgta
    13381 aatgctgttc aatctggtca agcgagtgct ctaatggccg gtacaaccat tacgaatgct
    13441 cgtaagaaag tctttcattt ctcagagcca tattacgata ccaaaattgt cattgcgaca
    13501 cgtaaagcca atgccatcaa aaaatacagt gacttaaaag gaaaaacggt cggtgttaaa
    13561 aatggaacag cggctcaagc ctttttgaat aactataaaa aaaagtatga ttatactgtt
    13621 aaaacatttg acacaggtga tcttatgtat aatagtttat ctgctggttc tattgccgct
    13681 gttatggatg atgaggcggt tatccaatac gcaatcagcc aaaaccaaga tattgctatt
    13741 aacatgaaag gagagcccat tggaagcttt gggtttgctg tcaaaaaggg aagcggatat
    13801 gattatctag ttaatgattt caatacagct cttaaagcta tgaaagctga tggtacctac
    13861 caagctatca tgaccaagtg gttaggcaca gatgataaag ctaccaccag tcaggcaacg
    13921 ggaaatccat ctgccaaagc tacacctata aaggacagtt ataaaattgt ctctgattcg
    13981 tcttttgcac cttttgaatt tcaaaatggt aagggcaaat acgttggtat tgacatagaa
    14041 ttaatcaaag ctattgctaa acaacaaggt ttcaaaattg aaatcgctaa tccaggtttc
    14101 gatgctgcct taaatgctgt gcaatctagc caagcagatg gggtcattgc tggtgcaact
    14161 attactgacg ctcgtaaagc tatctttgat ttttctgatc cttattatac ttctaatatc
    14221 attttagctg ttaaagctgg aaaaaacatc aagaactatg aagacttaga cagaaaaaca
    14281 gtcggtgcta aaaacggtac ttcatcttac tcttggttaa aagaaaacgc tcctaaatat
    14341 ggttataatg tcaaggcatt tgatgatggt tctagcatgt atgatagctt aaattcaggt
    14401 tctgtagatg ctatcatgga tgatgaggcg gttcttaaat acgctatctc tcaaggtcgt
    14461 cgctttgaaa cacctcttga gggcatttct actggtgaag ttggttttgc tgtcaagaaa
    14521 ggaactaatc cagaattaat cgaaatgttc aacaatggct tagctgctct caaaaaatct
    14581 ggtcagtatg atgacattat agataaatac cttgactcta agaaagctgc aactccttct
    14641 gaaaaaggtg ctgatgagtc tactatttca ggcctattat caaataacta caaacaacta
    14701 ttggcaggac ttggaaccac gctcagttta acccttattt catttgctat tgctataatt
    14761 atcgggatca tctttgggat gatggccgtg tcaccaacta aatcacttcg actcatttca
    14821 acggtctttg tggacgttgt tcgagggatt cctttgatga ttgtggctgc cttcattttc
    14881 tggggagtac caaaccttat cgagagtatg accggccacc agtcaccgat taatgatttc
    14941 ttagctgcta caattgcact gtcacttaat ggcggagcct atattgctga aattgttcgc
    15001 ggtggtatcg aagctgttcc agcagggcaa atggaagcta gtcgaagtct tggtttgtct
    15061 tacggaacca cgatgagaaa agtaattctc ccacaagctg tgaaactaat gttacctaac
    15121 tttatcaatc agtttgttat ttcattgaag gatacaacaa tcgtctcagc aattggttta
    15181 gtggaactct tccaaacagg taaaatcatt attgctagaa attaccagtc gttccgcatg
    15241 tatgctattt tagcaattat ttaccttatc atgattacac tcttaacaag acttgcaaaa
    15301 cgtttagaaa agaggcttaa ctaatgacag aattaaaaat tgatgttcaa gatttacaca
    15361 aatcttatgg tcaaaatgaa gtcttaaaag gaattgatgc caaattctac gaaggcgatg
    15421 tcgtttgtat cattggtcct tctggttctg ggaaatcaac ctttctacgt accctcaacc
    15481 tcctagagac gattaccagt ggtaaggtca tggtggacgg ttttgaatta tcagatccta
    15541 agactaatat tgacaaggca cgtgaaaata tcgggatggt attccaacac ttcaaccttt
    15601 tccctcacat gactgttctt gagaatatta tctttgctcc tgttgaatta ggtaaagagt
    15661 ctaaagaagt tgctaaaaag catggcatgg cacttctaga aaaagtcgga ctttctgata
    15721 aggccgacgc cttcccagga agtttatccg gcgggcaaaa acagcgtgtc gccatcgcca
    15781 gaagtttagc aatgaaccca gatattatgc tctttgatga accaacttct gctcttgacc
    15841 ccgaaatggt tggggacgtt ttgaacgtta tgaaagactt ggcagaacaa ggcatgacca
    15901 tgttaattgt tactcacgaa atgggatttg ctcgccaagt agctaaccgt gttatcttta
    15961 cagacggtgg tcaattcttg gaagatggta ctcctgaaga gatttttgat catcctaagc
    16021 accctcgtct cattgaattt ttagacaagg tcttaaacgt ctaatagagc agacaccata
    16081 ctaacttcca aaaaataata aggcaaggaa acaggtttcc ttgccttttt tggttactgt
    16141 tattattcaa accagcaaaa tagggatact cttatgactc tctccttcat aagagtgtcc
    16201 cctatgcttt tttatgggcg tttatgttta gtgaggtaat aacctaatcc cgtaagagct
    16261 agtcccgtca ctatgtgtaa gtatttgtta ccaaccatcg gactaatggc aatgagaaaa
    16321 aagccaacag ccattaaaaa agttgctcca tttttttctg tcatgcaagg tcactcgctt
    16381 tttctttatt ggccaaaatg acaaatggtg cccagataag gaaggcaaca aagaggttaa
    16441 acagtgaaac caaacctgcc ataatattac ctccagtagc taggtaagca taaagtccaa
    16501 caggggtaat ccaaggtact gataagaata ccggtggaat caaacctatt gcagtagcaa
    16561 aataagctat gctagcacaa attggtggca caattagcca tggaatcaca aaggttggat
    16621 tcaaaacaat tggcatccca aatgtaattg gttcgttgat attgaaaaca cccattggtg
    16681 cggacaattt ggcaatggtt ttatgctctt ctcgttttga gaatatgaag atagcaataa
    16741 tcaatgccaa ggttactcct gaaccaccca tttgagcata agcatcaaag gacccgcgtg
    16801 tccaaaggta tggtaaatta gcagcgctat gcgctgtatt gtaagcagct gtattttctg
    16861 ttaaagctac catataaata ccatccatca ctggggctaa gacattatga ccatgcaagc
    16921 cgaagaacca gagcagttga actaagaagg ttaacaagat aactgaacca ataccctgtg
    16981 ataagcctag caatggtaat tggatatagg ttgaaatgac atcacttagg gaagaacctg
    17041 ttaatgcgaa aatgaggtag gcgaaaattg ccgaagcgta aatcgcaact gttcctggaa
    17101 taatggcagc aaaggcttta ttaaccgcag gtggtacatt atctggtaag gtaatggtaa
    17161 tattacgttt ggtcaaagcc gcatacacaa aacttgataa aaagccaata attaaagctg
    17221 tgaacaagcc tgtggcaccg acatacttta gagctattgc tccccattga ctagtttcaa
    17281 tacttgaagc tccacccgca gtaacgactg aaagccccat ttctttcaaa ttgctaatta
    17341 ggttagcaga ggcattttca agtggtgtcg aaatggttaa agtctgaggt agggtggcaa
    17401 taaatgagcc aaaagaaatc aatcctcctg ccaatggatt aatcttatgc ataacagcta
    17461 aattgtaacc gaaagcaaag gcaaaaaaga gcgccatgat accaattgtc ccaaaataaa
    17521 catagccatt gatgtcgata acaggttgca tagcttttgc aaatcctgtc catcccatat
    17581 tgtttggaat atcgcgtaaa aaaacgttca ataagacagc aactgaacct gccatggtaa
    17641 taggcatcat tgaaataaag gaatcacgaa tagctactaa atgccgttgt gacccaattt
    17701 ttcctgaaat ctccataaac ttatcagaaa aatgcgacat aaatcttctc ctctttttat
    17761 ttttttaatg taacaaaggt aaccttaaaa gcagcggtgt ctgcctaaca caattatctc
    17821 aaaatcatcc ttgttaaacg tgatgcttag agataaccgg taatcggctc ttatccaaat
    17881 gaacccttct ctctcattca aggtctgtga ataatcttca caaagacgat ggttgctgca
    17941 actgtttagc atattggata agggctgcta actgagcttg agattggcgt tctaggcttt
    18001 ttttgaacag tttgccaaca ataaattgat ttgctttttg aaaaatatct agaggatgat
    18061 agtcataaga ataggtaatt ctagtccgat tctcccccag agcctctaat tgataagcca
    18121 attcctttat cccatgattg gaactaaaac aagcagtata taattgcgga gcttctaggg
    18181 ccgtaataag caccctcact gtacgttctt tgtatttacc ataatgttta acatagttta
    18241 tccccgcttt gatatcatct cttgacaact gtctcaaggt attttgtcga taatcttctt
    18301 ggagcgatat cagcatggct tcaaaacaat gatgaatgga aacatctcct tctgctgaaa
    18361 taatcatgac tcacctcttt gtattgctga cattttgtcg taaacggtaa ccaattcctt
    18421 ggctaaatca ataaaggcta aggcagtcat catgtgatct tgcccatgca ccatcaataa
    18481 agacaattca accgcatcac cagctgcttc ttgggacagc aaatgtgtct gcgatttatg
    18541 ggcaatattt aacgagtttg tcgcttcttg aagtaagtgc tgagcctcct caaactgccc
    18601 atctcgtgct gcatgaatag cctctacggc cttgcccttg gcttcgcctc catacatgat
    18661 taagcccata atagcttcta agttggatgg ttccgtcatg cctactcctc catcattgct
    18721 agggctgttt caaggacttt ttcaccgttc ataagaccat aatcagccat gttaatggca
    18781 tctactttaa tatctggttc aaacttatct ttaaaatcct ttagtaagaa tttgacttgt
    18841 ggacctaaca agagaacgtc aatgctatta gcagccactt cgttgtcagc ttcactcaca
    18901 ggaactgccc aaatagaaac ttcaaggtca cgcgcttggg ctgctttttg catcttagtc
    18961 accaacatac tagttgacat accggcatta caaactaaca taatagattt cataaggttt
    19021 ctccttttaa tcgttttaat catgaataag tggggtaaac actgtcatga atttggaata
    19081 atttggttct tctagtaacg cttgctgaag ctccacctga ttgacaaaag atgccaaacc
    19141 tggtgataca tattttaacc tatgattatg ccctttagac ggggataata gaaagacaaa
    19201 atgaacagct tgatgcgcct tgtcccactc aagaggctct cggcaaatag acactactac
    19261 ctgttcagaa taagtcatgg gattagcagg atgcggaaag gcaagcactt ctccatagat
    19321 gactgggctg taattttccc tcagtaccat ttgatgatag aagtcctcta caaacgtttc
    19381 tgttcccgcc tcgtcaagac aagcaatcat ccgtaaaagc aagtcttcct ttgagatttt
    19441 ctcgtctaaa taaagaaaac gattagggct aaaaacacct gttagaagct gctctgcttt
    19501 tgataaactc atctgtgacg gtaaactgac ctctttcttg ataccttctt gttctccaat
    19561 aaattgtcga atagtttcaa tatcctgatt tgacaaaaaa acactgacag taatcactgg
    19621 tgttaaaaac atcagattgg caaggctgat agaggaaata atcaaatcaa cggttttcag
    19681 tcgctcttcg gttatttcat agtaactgat aacatcgaca atacgcaaac gtccttcaaa
    19741 ttccttttcc aaccgatttt tcaacatcat ggcactacca taacctgtgg cacaaactac
    19801 tagaaccctc aatttatgac ggtttgaata acgctcaatg gccgccatta aatgcaaact
    19861 aatataagcc cattcatcat ctgagacatc attcttttga catacaagca aatccgaaaa
    19921 ggtctgcttg gtaagggtaa atatttcagg atactggctt ttaatttcct cagttaaagg
    19981 attagtcagc tgaatatggt tttcaagacg tgtgatcaag ggcatcatgt gggctaataa
    20041 accttgaaat aaattggtat ctgtttctaa agtcatgcct gtcaattgac taatcttggc
    20101 aatacatgct tttaaatggt cctgcaactt ttcatcggta ttatcatctt gccaatgctt
    20161 accaacagaa tgtttgacct ttagatgcag tgcaatataa ttagcctcct ctttaggaaa
    20221 acgtatcccc atcacttctt ccactcgata caaaatgcgc agagctactt gatactcatc
    20281 agactgacga atagctattg gaatggaaaa cagttctaat ggacaccccg atcgaatacg
    20341 ctgaaccatt aaggcaatat gcaagactag attatttatg acaaaatcag aaagtttcaa
    20401 cttagcatcc cgacattcat ccaagacaat aatcatgatt tctgcaaaat taataccttc
    20461 aagaaagata ttacctacca tggtggacaa ggtattatca aaactagtaa caaaaaaata
    20521 gtccatgata aaatgcctaa tatcctcttc tttacctctt atttgaatat gttggtgagt
    20581 tatttcaagc tctaactggt aaggtattat gcgctcctta atgcctgcta aaacatgttt
    20641 taaagttgtc cgactgatat acaattcttg acaaagctct tcaaaatcct gaacggcatc
    20701 ttcaaaaaag aatttatgaa gcacataatc ttctcgatca accgattctt ccacttgaga
    20761 cacatctgcc aaacgttttt tagacgtcac actttcttgc caaaagatgt caaatgccat
    20821 agaatggtca atttctaagc agtaacctcg accttgttta gaaatgatgt gagctccgtg
    20881 ttcaggaagg ctactgatca gttctttgag gtatttccta accgtacggt cactcatacc
    20941 aataacctca gccagagttg cactagagac aaactgctct ttgtgatgga ttagaaatgt
    21001 tatgatagcc agttcttttt ttgataacat tagtcctcct tactacttta gaaaacgctt
    21061 tctaccctct tttagtgtac caaatatttc ctgaaaaagg tgaccatcat tttccgcatt
    21121 gattgtgaaa agaatctatg atgggtcaat ctccaaacaa aattcctgcc aaggtcgcaa
    21181 atcatctaac aaactaagat agtcctctct gacctcagca accacattag cttttgaact
    21241 gaccgtaaaa tttttcaaag caatttgcag ttctccctta tatcgatgat agcggtcatt
    21301 gtcaataata atgctacctc gtttgacctc cttagattgg tctcttggag caatcgattc
    21361 ctgagcatag actagacgtg gcatggttga tcgaatcaca taatcagata catcgccacg
    21421 ataacaatgc ggatagccaa tgatttgctc ctcaacttct gtcaaatcga tgataggcct
    21481 aaccttgatc gttgtgactg gccttgctaa agcctgagta catgctgcca attcttcttc
    21541 cgaaatgaat tggttagata tgaggatatt atcaatggtt cctatcgctt tcattaattc
    21601 tacttgcagg ccaatgggca agtgcctatg gtcttccact gtcggcaagc cttctgccag
    21661 tagccaaggg ccttcagatg ctgactgagc ggtaataaaa gcagccgttt caatatcatg
    21721 ctcatgataa aaacgtgaca tatccttaaa atgttgccat gacaaaccgg taaattcatg
    21781 tggataaaaa ttatggcaac caataatatt gctccgttct gcatctgtcg ctagcaatga
    21841 catcaataat tgtttatcag tgctcatatt aagttcaatt ttaagcccaa agggatttct
    21901 tgttaaagtt acaatctctg ctagagggag agcctcgtct aagcgtaagc ccgctaaccc
    21961 gaaggcatga gctcgttcaa taagctgatc tgaccaacca gcttggctaa taaagcttgg
    22021 actaacgtca gcaatcactc gaatgcctaa ttgatttgca taagcaatga gctctgcgta
    22081 gcaatgaaac atctggtgat cagctggtgc cagctgtaag agactcataa acaagcgctt
    22141 ggctccatag ctatgacata agtcaatata agctttactc ttggtaacat cataccgctc
    22201 agggtataga gaaaatccta aatcaaccat attgcctcct atttatcaaa accatttgac
    22261 tcgctcaacg ccttaaacca gtaacctgat ttttttatgg ttcgcttttg actctctaaa
    22321 tccaaagcta ccaaaccata tcggttttta taagcattta accaagacca gcaatcaata
    22381 aaagtccaca agaggtagcc tttgcagttg gctccttctc ccaaagcctt atgaagctgg
    22441 ataagatgat cctctataaa ggtgatgcgg tagtcatctt gtatctggcc atctgctaaa
    22501 aaagcttctt caccttcgac acccatacca ttttccgtga ctagccattc gatgttacca
    22561 tagtgttctt ttaaattaag ggcaatatca taaagtcctg gttcataaat ttcccaaccc
    22621 cggtgaggat tgactttttt accaggcata tcataaggtt caaaataaat atctaagata
    22681 agggggtccc cctcttgctg tgacttacta ggtgcctgaa cccgcaaagg ctgataataa
    22741 ttaactccca aaaaatcaac agtattgttt ttgataatct ctaattcatc agcactatat
    22801 tgaggtaata aatcagcttc ctctaaaatg gaaactaact ctgctgggta aacacccaaa
    22861 actgatggat ctaggaagga tttggtttgg aaaagctctg cgattctagc agccttgaca
    22921 tcttcaggag cattactccg aggataagca ggtgtcatgt taagcacaat actaatcttg
    22981 tggtcgggat gaagcttatg acaagctttg actgccagtg aactggccag ttgtgtgttg
    23041 taagccactt gaactgctgc cttggcatct actttacaag ggtagtgata ataacctaaa
    23101 tagccacatt cgactggaac aatcggttca ttaaaggtaa tccaagtgtt tactaaatca
    23161 ccaaaaagct caaaacaggt ttttgcgtag gtttcataag cccagacagt agctttgttt
    23221 tcccagccac ctttttcttg caaggcatag gggaggtcaa aatgatagag gttaacaatc
    23281 aatttaatac cttgagcaat aatgtcttga aatacttctc gataaaatgt caccgccttt
    23341 ggattaacct ccccgactcc ttctggaatc aatcgagacc actggattga ggtccgaaaa
    23401 atcgtgtggc ctgtttcttt caaaagggca atgtcaccct tatagttttc ataaaaggtt
    23461 gaggtttttt ctggaccaat ttggtgatga aacttagctg actcaatact aaaccagtaa
    23521 tcccagttgc taggtccttt gccatctcca ggaaccgttc cttcgctttg aggcccagat
    23581 gtggaacttc cccataaaaa gccatctgga aattgataac ggtgttttct ttgtttcata
    23641 tcctttgtcc tttctttgct cacttctctt tttattataa gaagaaaaac aggtgatgta
    23701 aacgctttaa aaaatctgtt ttttccttat catcacggaa aataagtgat ttaaagcttg
    23761 acatatttta ttatcttaaa gagcctatct aacctgccct gctacttctt atcaaaacca
    23821 agcccctatt gacagatgcc ttagcctatg tttgaatagg ggtatggctt atttaaacta
    23881 ctttacaaaa accgagtggg cactttggtt aagctctgtc ttggccattt taattagcgc
    23941 ccttttattc ggtaagcaag ctcccttagc tttaatagct tctttgattg gtgtgacctc
    24001 tctcattttt agtgctaaag ctaatcctat tggtcaaggg ctcgtcatta ttttttccat
    24061 tatctatgcc tatctttcat tacgaaacag ttactatgga gagttaatga cctatctttt
    24121 catgaccctc cctatgacta ttttttcact ttttacttgg ttaaaccatc cttttgaagg
    24181 gaaaaaatca caagtgacta tttcacgatt aacacccact gatcgaagat gtcttttcgt
    24241 ttttactata ctggtcactc ttattttcta cggcatcttg gcttctattc aaacagccta
    24301 tttacttgtc tccactttat cgattgccac ttctttctca gccgtttatc tcagttataa
    24361 gcgtagccct tattttgctt tagcatacgg gctcaatgat ctagtcctca ttctcttatg
    24421 gatgcatgct gctcaaacag attctagcca atatgcagta gtcatctgct ttgccacctt
    24481 tcttattaat gatagttaca ctttttatag ttggctaatg ctccaacgtt accaagaaaa
    24541 aaaggtcaaa aaaggatgag ggcctaagcc tcatcctttt tcaatttttc acgttctaag
    24601 cgcaaacgtc tattgggatt taaaagatta aacaattctt ctaatttctc agcattccaa
    24661 acatctgctg ccgatacaaa atttccatcc ttatccttaa aggatatcgg tttatcaccc
    24721 ataatcagaa acctccatct taaagataac acacaagagc actatcacac tgctattgta
    24781 atggattgcc ttaatccaca aattcaaatt caaacttgcc aatacgaaca ggatccccat
    24841 cttttgctcc acgttcacga agggcttcgt caacccccat accacgcaat tgacgggcaa
    24901 atttcatgat ggactcatca cgttccatat tggtcatgac aaagagacgt tctaacttct
    24961 caccagacaa gacccaagta gcgtcatcgt cacgtgtgat ctcaaagtct ttttcagtct
    25021 cagcaaagcc atagtaagct tcttcatcca ccagatcagc ttcatcatat aataagaatt
    25081 cgtctgtttt agcgagtaat tctgccgttg cttctaacaa gttctctaag ccttgatgtg
    25141 ctaggcttga aatagggaaa atcattggca aatcattaaa ctcgtcatat tgggtagcca
    25201 attttttctt gaaggctttc aagttttctt gagcctctgg catgtccatt ttattagcca
    25261 caataatctg tgggcgctcc atcaatcgca aattataggt ttccagctcg ttattaatgg
    25321 aaacataatc ttcgtaagga tcacgaccct cactagcaga catgtcaatc acatgaagaa
    25381 taacccgtgt ccgctcgata tgacggagga attgagttcc caaaccaacc ccttggctag
    25441 caccttcaat caaacctggc agatctgcca tagcaaagct atcacctgac tttgtacgaa
    25501 ccattccaag attaggaaca atagtggtaa aatgataggc accaattttc ggtttagctg
    25561 atgacacaac acttaacaag gttgatttcc caacagatgg gaatccgacc aagccaacgt
    25621 cagctaagat tttgagttct aactctaact gacgttcctc acctggctca ccattttcag
    25681 caatctctgg cgcaggatta cgaggagttg caaaacgaat attcccacgg ccaccacgac
    25741 cacccttagc aatgaccact tcttgaccat gttctactaa gtctgtaatt acttttccag
    25801 tttcagcatc acgaaccgtt gtcccttgag gaacaaagac aatcaaatct tctgcaccac
    25861 ggccgtgcat gcctttggtc ataccttttt caccagattt cgccttgaat ttacgattat
    25921 accggaaatc catcaaggta cgcaaacctt cgtccactcg gaagataact gagccacctt
    25981 taccaccgtc tcctccccaa ggaccgccat ttgggacgta cttttcacga cgaaaagcta
    26041 ccataccatc gccaccacgg cctgcttgga cactgatttt tgccgtatct aaaaacatac
    26101 tcatactttt tttattctct aactttcttt tactttaaaa aaacgccttt c
//
LOCUS       DAUCLR010000022        25013 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.022, whole genome shotgun sequence.
ACCESSION   DAUCLR010000022 DAUCLR010000000
VERSION     DAUCLR010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25013
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.022"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            466..876
                     /locus_tag="VGT87_000702"
     CDS             466..876
                     /locus_tag="VGT87_000702"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEQ3813579.1"
                     /translation="MIREIITDHFLLQQKAQVAKKEDLWIGQDLQDTLAFYRQECLGM
                     AANMIGEQKRIVIVSMGFIDLVMFNPVMVSKKGIYQTKESCLSLSGYRKTQRYDKITV
                     EYLDHNWRPKRLSLTGLTAQICQHELDHLEGILI"
     gene            complement(958..2970)
                     /locus_tag="VGT87_000703"
     CDS             complement(958..2970)
                     /locus_tag="VGT87_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEQ3813580.1"
                     /translation="MKKYFILKSSVLSILTSFTLLVTDVQADQVDVQFLGVNDFHGAL
                     DNTGTAYTPSGKIPNAGTAAQLGAYMDDAEIDFKQANQDGTSIRVQAGDMVGASPANS
                     ALLQDEPTVKVFNKMKFEYGTLGNHEFDEGLDEFNRIMTGQAPDPESTINDITKQYEH
                     EASHQTIVIANVIDKKTNDIPYGWKPYAIKDIAINDKIVKIGFIGVVTTEIPNLVLKQ
                     NYEHYQFLDVAETIAKYAKELQEQHVHAIVVLAHIPATSKDGVVDHEMATVMEKVNQI
                     YPEHSIDIIFAGHNHQYTNGTIGKTRIVQALSQGKAYADVRGTLDTDTNDFIKTPSAN
                     VVAVAPGIKTENSDIKAIINHANDIVKTVTERKIGTATNSSTISKTENIDKESPVGNL
                     VTTAQLTIAKKTFPTVDFAMTNNGGIRSDLVVKNDRTITWGAAQAVQPFGNILQVIQM
                     TGQHIYDVLNQQYDENQTYFLQMSGLTYTYTDNDPKNSDTPFKIVKVYKDNGEEINLT
                     TTYTVVVNDFLYGGGDGFSAFKKTKLIGAINTDTEAFITYITNLEASGKTVNATIKGV
                     KNYVTSNLESSTKVNSAGKHSIISKVFRNRDGNIVSSEIISDLLTSTENTNNSLGKKE
                     TTTNKNTISSSTLPITGDNYKMSPIMTILALISLGGLNAFIKERKS"
     gene            3190..3840
                     /locus_tag="VGT87_000704"
     CDS             3190..3840
                     /locus_tag="VGT87_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEQ3813581.1"
                     /translation="MKERLEHYSTSSIYSGFEVYLPLVLQTITDVIIAENIKSKKETG
                     FKLYEHFTSRIKSEASMIEKCQRKQLPLTSKSALKIIKDSIGIRIICGFIDDIYRMVD
                     LLKSIPGMSVNTEKDYILNAKPNGYRSYHLILELETHFPDILGEKKGCYFIEVQLRTI
                     AQDSWASLEHQMKYKHQVANAEMITRELKRCADELASCDVTMQTIRQLIQETTEEE"
     gene            3843..4511
                     /locus_tag="VGT87_000705"
     CDS             3843..4511
                     /locus_tag="VGT87_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ3813582.1"
                     /translation="MKILLAEDEWQMSNVLTTAMTHQGYDVDVVFNGQEAIDKAKDNA
                     YDIMILDIMMPIKSGIEALKEIRASGNCSHIIMLTAMAEINDRVTGLDAGADDYLTKP
                     FSLKELLARLRSMERRVESFTPQVLQFAGVTLNINEQELSAGNAIRLASKEGKLMAFL
                     MLNQGKYLDTKTLYQHVWSDQEDYDTSIVWVYISYLRQKLLAIQANVIIAGDKDTSYC
                     LLEK"
     gene            4520..5752
                     /locus_tag="VGT87_000706"
     CDS             4520..5752
                     /locus_tag="VGT87_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEQ3813583.1"
                     /translation="MFNRIRIRFIMIASIAIFIILSSIVGIINTARCYQSQQEINRIL
                     HLISSNKGKLPDTTESSKRLGTKLSEDSLSQFRYYSVIFNANGHLLSSNTANISALDR
                     EEAQYFARLFAKSGEEKGSYRHQDSVYSYLITQLPNEEKLVVILDTTFYFRSVGDLLA
                     VSMMLAFGGFIFFVVLVSLFSGMVIKPFVQNYEKQRRFITNAGHELKTPLAIISANNE
                     LVELMTGESEWTKSTSDQVKRLTGLINQMITLARLEEQPDVVLHMVDFSAIAQDAAED
                     FKSLVLKDGKRFDLTIQPNIMIKAEEKSLFELVTILVDNANKYCDPKGLVKVSLTTIG
                     RRRKRAKLEVSNTYLEGKSIDYSRFFERFYREDESHNSKEKGYGIGLSMAESMVKLFK
                     GTITVNYKNDAIVFTVVI"
     gene            6045..6923
                     /gene="mvk"
                     /locus_tag="VGT87_000707"
     CDS             6045..6923
                     /gene="mvk"
                     /locus_tag="VGT87_000707"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEQ3813584.1"
                     /translation="MNENIGYGKAHSKIILIGEHAVVYGYPAIALPLTDIEVVCHIFP
                     ADKPLVFDFYDTLSTAIYAALDYLQRLQEPIAYEIVSQVPQKRGMGSSAAVSIAAIRA
                     VFSYCQEPLSDDLLEILVNKAEIIAHTNPSGLDAKTCLSDHAIKFIRNIGFETIEIAL
                     NGYLVIADTGIHGHTREAVNKVAQFEETNLPYLAKLGALTQALERAINQKNKVAIGQL
                     MTQAHSALKAIGVSISKADQLVEAALRAGALGAKMTGGGLGGCMIALADTKDMAEKIS
                     HRLKEEGAVNTWIQML"
     gene            6905..7849
                     /gene="mvaD"
                     /locus_tag="VGT87_000708"
     CDS             6905..7849
                     /gene="mvaD"
                     /locus_tag="VGT87_000708"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEQ3813585.1"
                     /translation="MDPNVITVTSYANIAIIKYWGKENQAKMIPSTSSISLTLENMFT
                     TTSVSFLPDTATSDQFYINGVLQNDEEHTKISAIIDQFRQPGQAFVKMETQNNMPTAA
                     GLSSSSSGLSALVKACDQLFDTQLDQKALAQKAKFASGSSSRSFFGPVAAWDKDSGAI
                     YKVETDLKMAMIMLVLNAAKKPISSREGMKLCRDTSTTFDQWVEQSAIDYQHMLTYLK
                     TNNFEKVGQLTEANALAMHATTKTANPPFSYLTKESYQAMEAVKELRQEGFACYFTMD
                     AGPNVKVLCLEKDLAQLAERLGKNYRIIVSKTKDLPDV"
     gene            7842..8855
                     /locus_tag="VGT87_000709"
     CDS             7842..8855
                     /locus_tag="VGT87_000709"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEQ3813586.1"
                     /translation="MSNYCVQTGGKLYLTGEYAILTPGQKALIHFIPLMMTAEISPAA
                     HIQLASDMFAHKAGMTPDASYALIQATVKTFADYLGQSIDQLEPFSLIITGKMERDGK
                     KFGIGSSGSVTLLTLKALSAYYQITLTPELLFKLAAYTLLKQGDNGSMGDIACIAYQT
                     LVAYTSFDREQVSNWLQTMPLKKLLVKDWGYHIQVIQPALPCDFLVGWTKIPAISRQM
                     IQQVTASITPAFLRTSYQLTQSAMVALQEGHKEELKKSLAGASHLLKELHPTIYHPKL
                     VTLVAACQKQDAVAKSSGSGGGDCGIALAFNQNARDTLISKWQEADIALLYQERWGEN
                     DQP"
     gene            8842..9831
                     /locus_tag="VGT87_000710"
     CDS             8842..9831
                     /locus_tag="VGT87_000710"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEQ3813587.1"
                     /translation="MTNRKDDHIKYALKYQSPYNAFDDIELIHHSLPSYDLSDIDLST
                     HFAGQDFDFPFYINAMTGGSQKGKAVNEKLAKVAAATGIVMVTGSYSAALKNPNDDSY
                     RLHEVADNLKLATNIGLDKPVALGQQTVQEMQPLFLQVHVNVMQELLMPEGERVFHTW
                     KKHLAEYASQIPVPVILKEVGFGMDVNSIKLAHDLGIQTFDISGRGGTSFAYIENQRG
                     GDRSYLNDWGQTTVQCLLNAQGLMDQVEILASGGVRHPLDMIKCFVLGARAVGLSRTV
                     LELVEKYPTERVIAIVNGWKEELKIIMCALDCKTIKELKGVDYLLYGRLQQVN"
     gene            complement(10061..11338)
                     /locus_tag="VGT87_000711"
     CDS             complement(10061..11338)
                     /locus_tag="VGT87_000711"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEQ3813588.1"
                     /translation="MTKTNLNWSGFSKKTFEERLQLIEKFKLLNAENLNQLKTDVLLP
                     IQTANQMTENVLGRLALPFSIAPDFLVNGSTYQMPFVTEEPSVVAAASFAAKLIKRSG
                     GFKAQTLNRQMIGQIVLYDIDQIDNAKAAILHKTKKLIALANKAYPSIVKRGGGARTI
                     HLEEKGEFLIFYLTVDTQEAMGANMVNTMMEALVPDLTRLSKGHCLMAILSNYATESL
                     VTTSCEIPVRLLDHDKTKSLQLAQKIELASRLAQVDPYRATTHNKGIFNGIDAVVIAT
                     GNDWRAIEAGAHAYASRNGSYQGLSQWHFDQDKQVLLGQMTLPMPIASKGGSIGLNPT
                     VSIAHDLLNQPDAKTLAQLIASVGLAQNFAALKALTSSGIQAGHMKLHAKSLALLAGA
                     TQDEIAPLVNALLADKPINLEKAHFYLSQLRQS"
     gene            complement(11325..12500)
                     /locus_tag="VGT87_000712"
     CDS             complement(11325..12500)
                     /locus_tag="VGT87_000712"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEQ3813589.1"
                     /translation="MTIGIDKIGFATSQYVLKLEDLALARQVDPAKFSQGLLIESFSV
                     APVTEDIITLAASAADQILTDEDRAKIDMVILATESSTDQSKASAIYVHHLVGIQPFA
                     RSFEVKQACYSATAALDYAKLHVASKPDSRVLVIASDIARYGVGSPGESTQGSGSIAL
                     LVTANPRILALNKDNVAQTRDIMDFWRPNYSFTPYVDGIYSTKQYLNCLETTWQAYQK
                     RENLQLSDFAAVCFHIPFPKLALKGLNNIMDNTVPPEHREKLIEAFQASITYSKQIGN
                     IYTGSLYLGLLSLLENSKVLQSGDKIGFFSYGSGAVSEFYSGQLVAGYDKMLMTNRQA
                     LLDQRTRLSVSKYEDLFYEQVQLDDNGNANFDIHLTGKFALTAIKEHRRIYHTNDKN"
     gene            12709..13548
                     /locus_tag="VGT87_000713"
     CDS             12709..13548
                     /locus_tag="VGT87_000713"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEQ3813590.1"
                     /translation="MTKADQIFKANIQKIINEGSLSEQARPKYKDGRTAHSKYITGAF
                     AEYDLAKGEFPITTLRPIPIKSAIKELFWIYQDQSNSLDVLEAKYNVHYWNEWEVDQT
                     RTIGQRYGAVVKKHDIISKILKQLAENPWNRRNVISLWDYEAFEETKGLLPCAFQIMF
                     DVRRVGEDLYLDASLTQRSNDILVAHHINAMQYVALQMMIAKHFGWKIGKFFYFVNNL
                     HIYDNQFDQAQELLKRQPVASQPKLVLNVPDGTNFFDIKPDDFELQNYDPVKPQLHFD
                     LAI"
     gene            13628..14125
                     /locus_tag="VGT87_000714"
     CDS             13628..14125
                     /locus_tag="VGT87_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEQ3813591.1"
                     /translation="MTKEIIAIWAEDEAGLIGVAGKLPWYLPKELEHFKKTTLHQAIL
                     MGRVTFEGMNCKRLPQRQTLVMTRNRDYQVDEVLTMTSIEKVLEWYHAQDKTLYIIGG
                     NKVLEAFNGYFDRIIKTVIHHRFKGDTYRPNLDFSHFTQESQTFYARDAKNPYDFTVT
                     ALKHK"
     gene            14145..14315
                     /locus_tag="VGT87_000715"
     CDS             14145..14315
                     /locus_tag="VGT87_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813592.1"
                     /translation="MQRSIFGVFTAFLCVICVLCAIPAFKKNRYGLASLFLLNAFTNL
                     VNSIHAFYGTLF"
     gene            14445..15674
                     /gene="clpX"
                     /locus_tag="VGT87_000716"
     CDS             14445..15674
                     /gene="clpX"
                     /locus_tag="VGT87_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEQ3813593.1"
                     /translation="MAGSRTNDIKVYCSFCGKSQDDVKKIIAGNNVFICNECVALSQE
                     IIKEELAEEVLADLTEVPKPKELLDVLNQYVVGQDRAKRALSVAVYNHYKRVSFTESR
                     DDDDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLIQAADYNVERAERGIIYVDEIDKIAKKGENVSITRDVSGEGVQQALLKIIEGTV
                     ASVPPQGGRKHPNQEMIQIDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNSRKID
                     DNASYMQEIISEDIQKFGLIPEFIGRLPVVAALEQLNTSDLIQILTEPRNALVKQYQA
                     LLSYDGVELAFDKEALEAIANKAIERKTGARGLRSIIEETMLDIMFEIPSQEDVTKVR
                     ITKAAVEGKSKPVLETA"
     gene            15684..16283
                     /locus_tag="VGT87_000717"
     CDS             15684..16283
                     /locus_tag="VGT87_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEQ3813594.1"
                     /translation="MAEEQVLNTHNASILLSAANKSHYPQDDLPEIALAGRSNVGKSS
                     FINTILGRKNLARTSSKPGKTQLLNFFNIDNKLRFVDVPGYGYAKVSKSERAKWGKMI
                     EEYLTSRDNLRAVVSLVDLRHAPSKEDIQMYDFLKYYDIPVIVVATKADKIPRGKWNK
                     HESVVKKALNFDKSDTFIVFSSVERIGIDDSWDAILEQV"
     gene            16431..17174
                     /locus_tag="VGT87_000718"
     CDS             16431..17174
                     /locus_tag="VGT87_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813595.1"
                     /translation="MTQVAQGRSKGKSLLCHQCNALFVFIIFLILLAVLFGVRYRNSS
                     IEGIWRTTSIDQKLGDDFAKRLTGLHQSPLIDDSLLTSSQMILTVKNNNVDLSFSVQV
                     ERDIFVKRLAAYHQNELLKTLKENHLVVGDLSSKERQIIENSMPASHELEMILDQAFE
                     KLASQIGGKYNQKTGHLSAVVLKGKVNRILHTIDIKEEVAAGHTSFSKGLLTPNGYFD
                     YTRFGKKLELLGDEKIIFKKALKKSPSSV"
     gene            complement(17232..19331)
                     /locus_tag="VGT87_000719"
     CDS             complement(17232..19331)
                     /locus_tag="VGT87_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEQ3813596.1"
                     /translation="MTHFSGKDPFVNMDDIFNQLMANMGGYRSENPRYLVNGREITPE
                     EFQHYRQTGQLPVATTKATNSQMLTPKADSVLTQLGTNLTQEARQGHLDPVIGRNKEI
                     QDTAEILARRTKNNPVLVGDAGVGKTAVIEGLAQAIVNGDVPAAIKNKEIVSIDISSL
                     EAGTQYRGSFEETIQNLIQEVKEAGNIILFFDEIHQIVGAGATSSDSGSKGLADILKP
                     ALSRGELTLIGATTQDEYRNTILKNAALARRFNEVKVNAPSAEDTFHILMGIRNLYEQ
                     HHHITLPDNVLKAAVDYSIQYIPQRSLPDKAIDLLDMTAAHLAAQHPVTDLKTLETEI
                     AKQKERQEKAVAKEDFEKALAAKTRIETLQKQIEQHNQTQNVTATVNDIAESVERLTG
                     IPVSNMGTNDLERLKGISSRLKSHVIGQDEAVAAVARAIRRNRAGFDDGNRPIGSFLF
                     VGPTGVGKTELAKQLALDLFGSKDAIIRLDMSEYNDRTAVSKLIGTTAGYVGYDDNNN
                     TLTERVRRNPYAIVLLDEIEKADPQIITLLLQVLDDGRLTDGQGNTINFKNTVIIATS
                     NAGFGQQDTETSGFNIMDRIAPYFRPEFLNRFNSIIKFNHLQKENLEEIVDLMLAEVN
                     QTTAKKGISLTIADDAKAHLIDLGYNRAMGARPLRRIIEQEIRDRITDYYLDHPEVKK
                     LQAILKEGQLVIRQNDQ"
     gene            19709..20392
                     /gene="rpiA"
                     /locus_tag="VGT87_000720"
     CDS             19709..20392
                     /gene="rpiA"
                     /locus_tag="VGT87_000720"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEQ3813597.1"
                     /translation="MEALKKIAGVTAAQYVTDGMTIGLGTGSTAYYFVEEIGRRVKQE
                     GLQVVGVTTSSVTSKQAEGLGIPLKSIDDIDSIDLTVDGADEVDKDFNGIKGGGAALL
                     MEKIVATPTKEYIWVVDASKMVEHLGAFKLPVEVVQYGADRLFRVFEKAGYKPSFRMK
                     GDSRLVTDMQNYIIDLDLGCIKDPVAFGHLLDGTVGVVEHGLFNGMVDKVIVASKDGV
                     TVLEAPKAG"
     gene            20469..21680
                     /locus_tag="VGT87_000721"
     CDS             20469..21680
                     /locus_tag="VGT87_000721"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEQ3813598.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDADKFFNAGVADTDSDTLGHISE
                     TAGLSVPNMAKIGLGNISRPIPLKTIPTEDNPTGYVTKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKIIREANKPYSGTAVIDDFGPRQMETGELIV
                     YTSADPVLQIAAHEDIIPVEELYKICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRHDYAVSPFQDTVLNKLADAGVPTYAVGKINDIFNGSGITNDMGHNKSNSHGIDTL
                     IKTLQLPEFTKGFSFTNLVDFDANFGHRRDPEGYRDCLHEFDNRLPEIFANMKEDDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSASFTGNGLIPQGHFADISATVAENFGVDTA
                     MIGESFLGHLK"
     gene            21699..22139
                     /gene="arsC"
                     /locus_tag="VGT87_000722"
     CDS             21699..22139
                     /gene="arsC"
                     /locus_tag="VGT87_000722"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922177.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEQ3813599.1"
                     /translation="MEEITIYHNPNCGTSRNVLAMIRHAGIEPTIIEYLQTPPNRETL
                     IELLQSMGISARELLRTNVPEFEAHGLANQAVAEKDVINAMLADPILINRPIVVTRKG
                     VKLCRPSETLLDILPVPLPSPYMKEDGESVNPIEVKGEKDVIDD"
     gene            22123..22932
                     /locus_tag="VGT87_000723"
     CDS             22123..22932
                     /locus_tag="VGT87_000723"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEQ3813600.1"
                     /translation="MSLMTKINETKDFLVTKGIETPEFGLILGSGLGELAEEVENAIV
                     IDYADIPNWGKSTVVGHAGKLVYGDLAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKA
                     LGCEGVLVTNAAGGIGYGPGTLMAITDHINMTGNNPLIGENLDEFGPRFPDMSDTYTK
                     VYRDKAHEVAEKMNIKLEDGVYMGLTGPTYETPAEIRAFKVLGADAVGMSTVPEVIVA
                     AHSGLKVLGISAITNFAAGFQSELNHEEVVEVTQHIKEDFKGLVKAILAEL"
     gene            23598..24311
                     /gene="deoD"
                     /locus_tag="VGT87_000724"
     CDS             23598..24311
                     /gene="deoD"
                     /locus_tag="VGT87_000724"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEQ3813601.1"
                     /translation="MSIHISAKKGDIADKILLPGDPLRAKFIAENFLEDAVCFNEVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYARELIVDYGVKTLIRVGTAGAIDPEVHVRE
                     LVLAQAAATNSNIIRNDFPEFDFPQIADFGLLDKAYHIAREMGVTTHVGNVLSSDVFY
                     TNMPERNMALGKLGVKAIEMEAAALYYLAAQHHVKALGIMTISDNLNDPTEDTTAEER
                     QTTFTDMMKVGLETLIAND"
     gene            24304..>25013
                     /locus_tag="VGT87_000725"
     CDS             24304..>25013
                     /locus_tag="VGT87_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HEQ3813602.1"
                     /translation="MTNNQTLDILLDVYAYNHAFRIAKALPNIPKTALYLLEMLKERR
                     ELNLAFLAEHAAENRTIEDQYHCSLWINQSLEDEQIANYILDLEVKVKNGAIIDFVRS
                     VSPILYRLFLRLITSEIPNFKAYIFDTKNDQYDTWQFQAMLESDHEVFKAYLSQKQSR
                     NVTTKSLADMLTLTSLPQEIKDLVFLLRHFEKAVRNPLAHLIKPFDEEELHRTTHFSS
                     QAFLENIITLATFSGVIYR"
BASE COUNT         7769 a         4423 c         5047 g         7774 t
ORIGIN      
        1 cttttctatt attacgtgag gaaaaatcaa gtaaaaaagt taagtcaact gaaaaacttg
       61 acagaaatat tcagaaaatt tataatacta actattaaca acatatcagc tattaaagca
      121 tttcaaacag caggtgtttt atttggacct gttaaggctg caaatgcaag agaaagcagt
      181 atctggtttg aaaatctcac aaagcaataa ctatctctct tagacatttg aagaagcaga
      241 cagtaaattc gataatattg tgatgagaac ttagcaacaa tctgctgatg caggtcaatt
      301 tttatttggt actcatattg cagaatttca taaagttgcc caagatatct tgaaataagg
      361 aatcacctga tgttttaaac agatgatact aagtctatca tatggtgata atagtccatt
      421 gaacatgtag tttatgaatt aaaaagaagg tttaaggaga taagaatgat tagagaaatc
      481 ataacagatc attttttatt acagcaaaaa gcgcaagtcg ccaagaaaga agatctatgg
      541 attggccaag atttacaaga taccttagcc ttttatagac aggaatgtct tgggatggct
      601 gccaatatga ttggtgaaca aaagcgcatt gttattgtga gtatgggatt tattgatttg
      661 gttatgttta atcctgtgat ggtttctaaa aagggaattt accaaactaa agaatcttgt
      721 ttgtcgttat ctggctatcg caaaacacag cggtatgata agattactgt tgagtatttg
      781 gaccataatt ggcgaccaaa gcgattaagc ttaacaggct taactgctca aatatgccag
      841 cacgaattag atcatctaga aggtatttta atttaacaaa aaacctagag taggacgctt
      901 taggtttttt tggcaaagat gcctgattat ataagggtga tcgattacta gtaaacgcta
      961 ggatttcctt tctttaataa aagcgtttag tccacctaag cttatcaagg caaggattgt
     1021 cataatagga gacattttat aattgtcccc tgttattgga agagtggaac tagagatagt
     1081 atttttgttt gtcgttgttt cttttttgcc aaggctgtta ttagtgtttt cagtagaagt
     1141 caaaaggtct gaaatgattt cactagacac tatattgcca tcacgatttc taaaaacctt
     1201 actaatgata ctgtgtttac cagcactatt aacttttgtc gagctttcaa ggtttgaagt
     1261 tacataattt ttaacccctt ttatagtagc attaacagtt ttacctgatg cttctaaatt
     1321 tgtgatatat gtgatgaaag cttcagtatc tgtgttaata gctccgatta atttagtttt
     1381 tttaaatgct gaaaagccat caccaccacc ataaagaaag tcgttgacaa caacggtgta
     1441 agtagttgtt aagttaattt cttcaccatt gtctttataa accttaacta tcttgaaggg
     1501 ggtatcagag ttcttaggat cattatctgt ataagtgtat gttaaacctg acatttgaag
     1561 aaaataggtc tggttttcat cgtattgctg atttaggaca tcgtaaatgt gttgaccagt
     1621 catttgaatg acttgaagga tattaccaaa tggttgtaca gcctgtgcag ctccccaggt
     1681 gatggtccgg tcatttttga caactaggtc acttcgaata ccaccattat tggtcatagc
     1741 aaagtcaaca gttggaaaag ttttcttagc aatagtaagc tgagccgttg ttactaagtt
     1801 accgacagga gattctttat caatattttc tgttttagaa atagttgaag aattagttgc
     1861 agttccgatt tttcgttcag taactgtttt aacaatatca ttagcatgat ttattatagc
     1921 tttgatatct gaattttctg ttttgatacc tggtgctaca gcaacaacat ttgctgatgg
     1981 agttttaata aaatcattgg tatcagtatc tagcgtacca cggacatctg cataagcttt
     2041 tccttgagag agggcttgaa cgatacgtgt tttaccgata gttccattag tgtattgatg
     2101 attatgtcct gcaaaaataa tatcaatgct atgttcggga tagatttggt tcactttttc
     2161 cataaccgta gccatttcat gatcaacaac accatcttta cttgttgcag gaatatgagc
     2221 taaaaccaca atagcatgaa catgttgttc ttgcagttct ttagcatatt tggcaatggt
     2281 ttcagctaca tctaaaaatt gatagtgttc atagttttgc tttaaaacga gatttggaat
     2341 ctctgtagtc acaacaccaa tgaagccaat cttaacgatt ttgtcattaa tggctatgtc
     2401 ttttatagca taaggtttcc aaccataggg gatatcatta gtttttttat caatgacatt
     2461 agcaatgacg atggtttgat gcgaagcttc gtgctcatat tgtttggtga tatcgttaat
     2521 tgttgattca ggatcaggcg cttgacctgt catgatacgg ttaaattcat ctagtccttc
     2581 gtcaaattca tgattaccaa gagtgccata ttcaaatttc attttgttaa agactttcac
     2641 agtaggctca tcttgtaaaa gtgcagagtt agcaggactg gctccgacca tatctccagc
     2701 ttgaacacgt atacttgttc cgtcttgatt tgcttgcttg aaatctatct cagcgtcatc
     2761 catataagca cctaattgag cagccgtccc agcatttggt attttaccac ttggtgtgta
     2821 agctgttccg gtattatcaa gagcgccgtg aaaatcattg acgccaagga attgcacatc
     2881 aacttgatct gcttgaacgt ctgtaactaa tagagtaaaa ctagtcagga tactcaatac
     2941 actacttttt aaaataaaat attttttcat aaaatctcct aataagttaa cgtaatcgtt
     3001 atagatcaag tttactatgt ttttgtttgt aattcaatgg tatttttaaa aaataacgta
     3061 caaaaaatat atgaaagcga tttaaaagca aacgatacag atcatttgca gttatatagt
     3121 tagaaataaa gataaccgat cattgcagag atttaagcat ttgttgagaa aatttgttat
     3181 aataagcgta tgaaagaaag actagaacat tattctacca gctctatata tagcggattt
     3241 gaagtgtact tgccgttggt actacagaca ataacagatg tgattattgc tgagaatatc
     3301 aaaagcaaaa aagaaacagg gtttaaacta tacgaacact ttacatcccg tattaaatcg
     3361 gaagcttcga tgattgaaaa atgtcaacgc aaacagcttc ctctaacaag caaatctgcg
     3421 ttaaaaatca ttaaagatag tatcggtatt cgcattattt gtggttttat tgatgatatt
     3481 tatcggatgg tagacttgct caaaagcatt cctggtatgt cagttaacac tgaaaaagat
     3541 tatattttaa atgccaagcc taatggctat agatcatatc atctcatttt ggaattagaa
     3601 acacattttc cagatatttt aggggaaaaa aaaggatgtt attttataga agtgcagttg
     3661 cgaaccattg ctcaagactc ttgggctagc ttggaacatc aaatgaagta caaacatcaa
     3721 gttgctaatg cagaaatgat tacaagagag ttaaagcgtt gtgcagacga gttggcatct
     3781 tgtgatgtta ccatgcaaac cattcgtcaa ttgattcaag aaacgacgga ggaagagtaa
     3841 ccatgaaaat tttattagct gaagacgaat ggcagatgtc taatgttttg acaactgcca
     3901 tgactcatca aggttatgac gttgatgtgg tttttaacgg tcaagaggca attgataaag
     3961 ccaaagacaa tgcatatgat attatgattt tagatatcat gatgccgatt aagtctggca
     4021 ttgaggcttt aaaggagatt cgcgcttcag gaaattgctc acatattatt atgctgacgg
     4081 ctatggcaga aattaatgac cgtgttaccg gactagatgc gggagctgat gattatctga
     4141 ctaaaccttt ctcattaaaa gaacttttag ctagattaag gtcgatggaa agacgagtag
     4201 aaagttttac tcctcaggta ttacaatttg ctggtgtcac attaaatatt aatgagcaag
     4261 aactgagtgc cggtaatgct atacgattag cttctaaaga aggtaaatta atggcctttt
     4321 taatgttaaa tcaaggaaag tacttggata ctaaaacact ttaccaacat gtttggtcag
     4381 accaagaaga ttatgatact tctattgttt gggtttatat ttcttatctt cgtcaaaaat
     4441 tattagctat tcaagcaaat gtcattattg caggtgataa agatactagc tattgtttat
     4501 tagaaaaata ggaggtgaca tgtttaatcg tatccgtatt cgttttatca tgattgcttc
     4561 tattgccatt tttattattt taagttcaat tgttggtatt attaacacgg cacgctgtta
     4621 ccaaagtcaa caagaaatta accgtatttt acatttaatt tcctcaaata aaggtaaatt
     4681 acctgacact acggaaagct ctaaacgact tggaacgaaa ctgtctgaag atagcctaag
     4741 ccagtttcgg tattatagtg tcatttttaa tgctaatgga catctacttt cttctaatac
     4801 tgctaatatt tcagctttag atagagaaga agcccaatat tttgctagac tttttgctaa
     4861 gtctggggaa gaaaaaggca gttaccgtca ccaagatagt gtttattcgt acttgattac
     4921 tcagctgcca aatgaagaaa aactggttgt tattttagat actacctttt attttcgtag
     4981 tgtaggagat ttattagctg tttcaatgat gttggccttt ggaggattta tcttttttgt
     5041 tgttttagtg agtctttttt ccggtatggt catcaaacct tttgtccaaa attatgagaa
     5101 gcagcgccgc tttattacta atgcaggcca cgagttaaaa acaccattag ccattatctc
     5161 agccaataat gaattggtcg aattaatgac tggagaatca gaatggacca agagtacaag
     5221 tgatcaggtg aagcgattga caggcttgat taatcaaatg attactttgg ctcgtttgga
     5281 ggaacagcca gatgtggtat tacatatggt agacttttca gctattgctc aagatgcagc
     5341 tgaagatttt aagagtctgg ttttgaaaga tggtaaacgt tttgatttga cgattcagcc
     5401 gaatattatg attaaagcgg aggaaaagtc attatttgaa cttgtgacaa ttttagttga
     5461 taatgccaat aaatattgtg atcctaaagg cttggtcaag gtatctttaa ccactattgg
     5521 aagaagacga aaacgagcga aattagaagt ttctaatacc tatttggaag gaaaatccat
     5581 tgattacagc cgcttttttg aacgctttta tcgtgaagac gaatcccata atagcaaaga
     5641 aaaaggctat ggaattggtt tatctatggc agaaagtatg gttaagttat ttaaaggaac
     5701 gataactgta aattataaaa acgatgctat tgtttttaca gtggtgattt aacttaaaca
     5761 tttttcagta cctttagcta gtaataatgt ctaggcaaag gtaataaatg atgcttaacc
     5821 agcccatgag gctggtttta acgtttaatt tagtagaaga gtgattaaag aaggtagttt
     5881 gaaataagaa aatttttgtt atgatcctaa aaagagtaga tagtctttat tgttaatttc
     5941 agtgaagaaa tgattttaaa aagatatatc atcgcatttt ctaatcattt agtgtaaaat
     6001 agtaaggtaa aagtttagaa gaattagtaa tgaaaagata aaaaatgaat gaaaacattg
     6061 gatatggtaa ggcacacagt aagatcattt tgataggtga gcatgctgtt gtgtatggct
     6121 acccagctat tgctttgcct ttaacagata ttgaggtggt ttgtcatatt tttccagctg
     6181 ataagccatt ggtctttgat ttttatgata ctctatcaac agccatttat gctgccttgg
     6241 attatttgca gcgactacaa gaaccaattg cttatgagat tgtttcacaa gtgccacaaa
     6301 agcgtggtat ggggtcttcg gcagccgttt ctattgcagc tattagagcc gttttttctt
     6361 attgtcaaga acccctctcg gatgatttgt tggaaatttt agtcaacaag gcagaaatta
     6421 ttgcccacac caatcctagt ggtttagatg ccaagacatg ccttagtgac catgccatta
     6481 agtttatccg aaacattggc tttgaaacta tcgaaatcgc cttaaatggt tatcttgtca
     6541 ttgcagatac agggattcac ggtcatacac gtgaggccgt caataaggta gcacagtttg
     6601 aggaaacgaa tttgccctat ctggctaaac taggagcttt gacacaagcc cttgaaagag
     6661 cgattaacca aaaaaataag gtggctatcg gtcagctcat gacacaagcg cactctgctt
     6721 taaaggccat tggcgttagc atcagtaaag ctgaccaact tgtggaggct gccctaagag
     6781 caggtgcttt aggcgctaag atgacaggcg gtggcttggg tggctgtatg attgccctag
     6841 cagataccaa agacatggcc gaaaaaatca gtcacagatt aaaagaagaa ggagccgtaa
     6901 acacgtggat ccaaatgtta taaccgtaac atcctatgct aacattgcta ttatcaaata
     6961 ttggggaaaa gagaatcaag ctaagatgat tccttcgacc tctagcattt ctttgacttt
     7021 ggaaaacatg ttcaccacaa ccagcgtttc cttcttacca gatactgcaa ccagtgatca
     7081 gttttacatt aacggtgtct tgcaaaatga cgaagaacat accaaaattt ctgctatcat
     7141 tgaccaattt cgccaacctg gtcaggcttt tgtaaagatg gaaactcaaa ataatatgcc
     7201 aacagctgca ggtttatcat caagttccag tggcttatca gccttggtta aagcctgtga
     7261 tcaattattt gacacccagc tagatcagaa agctttagct caaaaggcta agttcgcctc
     7321 aggatcatct tctcgttctt tttttggccc agtcgctgct tgggacaaag atagtggtgc
     7381 tatttacaag gttgagactg acttgaaaat ggccatgatt atgctggtct taaatgctgc
     7441 gaaaaagcca atttctagcc gagagggcat gaagttatgc cgcgatacct caactacatt
     7501 tgatcaatgg gtagaacaat cggcaatcga ttaccaacat atgcttacct atctcaaaac
     7561 taataatttt gagaaagttg gtcagttaac agaagctaat gccttggcca tgcatgccac
     7621 gacaaagaca gccaatcctc ccttttctta cctaacaaaa gagtcttacc aggctatgga
     7681 ggctgtgaaa gaactgcgtc aagaaggttt tgcttgttat tttaccatgg acgcaggtcc
     7741 aaatgtgaag gtcctatgtt tagaaaaaga cttggctcaa ctagcagaac gacttggcaa
     7801 gaactaccgt attatcgttt caaaaacaaa ggatttacca gatgtctaat tattgtgtgc
     7861 aaacaggtgg gaaactatac ctcacaggcg aatatgctat cttaacacca ggacaaaaag
     7921 ccttaattca ctttattcca ctgatgatga cagcagaaat tagcccagca gcccatattc
     7981 aattagcttc agatatgttt gcccataaag cgggcatgac acccgatgcc tcttatgcac
     8041 tgattcaagc aacggttaaa acctttgctg attatctagg acagtcaatt gaccaactgg
     8101 agccattttc cctaattata acgggaaaaa tggagcgcga tggcaaaaaa tttggcattg
     8161 gttcaagtgg tagtgtcacc ctcttaacct taaaggcctt atcggcctat tatcagatca
     8221 ctttaacccc agagttactc tttaaactag cggcttatac cttgcttaag caaggagata
     8281 atgggtctat gggggatatt gcctgtatcg cttaccagac tttagtcgcc tatacctcct
     8341 ttgaccgaga acaggtcagt aactggctgc agaccatgcc cttaaaaaaa cttctcgtca
     8401 aagattgggg ttaccatatc caagtcattc aaccagccct gccttgtgac tttttggttg
     8461 gctggactaa aatacctgct atttcaaggc agatgattca acaagtgaca gcgagcatta
     8521 ccccagcttt cttaagaaca agttaccagc taacgcaatc agctatggta gctttgcaag
     8581 aaggtcataa ggaagaactc aagaaaagtt tagcaggagc aagtcatctc ctaaaagagc
     8641 ttcatccgac tatctaccat cctaagctag taaccttggt agctgcttgt cagaagcaag
     8701 atgctgttgc taaatcttca ggttctggtg gtggagattg tggcatcgcg cttgccttta
     8761 atcagaatgc cagagatacc cttatttcca aatggcaaga agctgatatc gcattacttt
     8821 atcaagaaag gtggggagag aatgaccaac cgtaaagatg atcacatcaa atatgccctc
     8881 aagtaccaat cgccttataa tgcttttgat gacatagaac tcatacatca ttccttacct
     8941 agctatgatt tgtctgatat tgatctcagt actcattttg ccgggcaaga cttcgacttt
     9001 cccttttaca tcaatgccat gacaggagga agtcaaaaag gcaaagctgt caatgaaaaa
     9061 ttggccaaag tagcagcagc aacagggatt gtcatggtga cagggtctta tagcgctgct
     9121 ttaaaaaatc ctaacgacga ttcctatcgt ttacatgagg tggcagataa cttgaaacta
     9181 gccacgaata ttggtctaga taaacctgtg gcgctaggac aacaaacggt tcaagaaatg
     9241 cagcccctct ttttacaggt tcatgtgaat gtgatgcaag agttgctgat gccagagggt
     9301 gagcgcgtct ttcatacctg gaaaaaacac ctcgctgaat acgctagtca aataccagtt
     9361 cctgtcattc tcaaagaagt tggttttggc atggatgtca atagtatcaa gctagcacat
     9421 gacctaggca ttcaaacctt tgatatttca ggaagaggag ggacctcatt tgcctatatt
     9481 gaaaatcaaa gaggaggaga ccgctcttac ttaaacgatt ggggacaaac caccgttcag
     9541 tgcttactga atgcacaagg actgatggac caagtggaaa tcttagcttc gggtggtgtc
     9601 agacacccct tggacatgat taaatgtttc gtcctaggag cacgtgcagt gggactctca
     9661 cgcaccgttt tagaattggt cgaaaaatac ccaaccgagc gtgtgattgc tatcgttaac
     9721 ggctggaaag aagaattaaa aatcattatg tgtgctcttg actgtaaaac tattaaagaa
     9781 ttaaagggag tcgactactt actatatgga cgcttgcagc aggtcaatta ggcttcatca
     9841 cctctacaat agtgtcacct actacaaaga tgatagagct tttttagaac ataatataaa
     9901 atatttataa atacctcatc aaactcagaa tagagtagat gagtaaatag agtatgttat
     9961 ggagactatt tctaagactt gtatgtatag aaacaaagat taaacaccca tgtaaaccaa
    10021 ttgtttacat gggtgtttgc cttatattag ttgtttaaat ctaagactgt cttagctgag
    10081 ataagtaaaa atgtgctttt tctagattta ttggtttatc agctagtaaa gcattaacta
    10141 aaggagcaat ttcgtcttgg gttgcccccg ccaaaagagc taatgatttc gcatgtagtt
    10201 tcatgtgacc agcttggatg ccagatgagg tcagagcttt tagtgcagca aagttttgag
    10261 ctaaccccac agatgcaatc aattgggcta atgttttggc atcaggttga ttaagaagat
    10321 catgtgcgat agaaacagta gggttaagcc cgatagatcc ccccttacta gcaataggca
    10381 tagggagggt catttggcca agcagaactt gtttatcttg gtcaaaatgc cactgactaa
    10441 gtccttgata gctaccattt cttgaggcat aagcatgggc ccctgcttca atagcacgcc
    10501 agtcatttcc tgtggctatt accactgcat caatgccatt aaaaatacct ttattatgag
    10561 tagtagcccg gtaaggatct acttgagcta gtcggctggc tagctctatt ttttgagcta
    10621 actgtaggga ttttgtttta tcgtgatcta aaaggcgcac gggaatctca caactagtag
    10681 taacaagcga ctctgttgcg taattagata aaatcgccat tagacaatgc cccttagaca
    10741 gtcttgttaa atcaggaaca agagcttcca tcatagtatt gaccatattt gctcccatag
    10801 cttcttgggt atcaacagtc agatagaaaa tcaaaaactc tcctttttct tccaaatgaa
    10861 tggttctagc gcctccacct cttttaacaa tggaaggata agctttattt gccaatgcaa
    10921 ttagcttttt tgttttatga aggatggcgg ctttagcgtt atctatttgg tcgatatcgt
    10981 aaagaacaat ttgaccaatc atttgtcggt ttagggtttg agctttaaaa ccacctgagc
    11041 gtttgattag ttttgctgcg aaagatgctg cagcaacaac agaaggttct tccgtgacaa
    11101 aaggcatctg ataagttgaa ccgttgacaa gaaaatcagg agctatgcta aagggcaaag
    11161 ccaatcgtcc taagacattt tcagtcattt gattagctgt ttggataggc aaaagaacgt
    11221 ctgttttgag ttgatttaag ttttcagcat taagtagttt aaatttttcg ataagttgga
    11281 ggcgttcttc gaatgttttc tttgaaaaac cgctccagtt aagattagtt tttgtcattg
    11341 gtatgataga tccttcgatg ctccttgatg gctgttagag caaattttcc agttaagtga
    11401 atgtcaaaat tggcattacc attatcatct aattggactt gttcgtagaa aaggtcttcg
    11461 tatttagaaa cggaaagacg tgttcgttga tccagtaaag cttgtcgatt agtcattaac
    11521 attttatcgt acccagcaac taactgaccg gaataaaatt cacttacggc accggaacca
    11581 tagctaaaaa aaccaatttt atctccagat tgtaatactt tactattttc aagtaaagat
    11641 aacaatccta gataaagtga gccagtataa atatttccaa tttgtttgct ataagtaata
    11701 gaagcttgaa aggcttctat tagtttttct ctgtgttcag gaggtactgt gttatccata
    11761 atgttattta gaccttttag ggctaacttg ggaaatggaa tatggaaaca aactgcagca
    11821 aaatcagata actgaaggtt ttctcttttc tgataagctt gccacgtggt ttctaagcaa
    11881 ttgagatatt gcttggtaga gtaaatacca tcaacataag gtgtgaaact atagttaggc
    11941 ctccaaaagt ccataatatc cctagtttga gccacattat ctttatttag agcaagaata
    12001 cgagggttag cagttaccaa aagagcaata ctaccagagc cttgagtcga ttccccagga
    12061 gatcctacac cgtatctagc aatatcacta gcaataacaa ggacacgaga atctggctta
    12121 gaagccacat gcagtttagc atagtctaga gcagcagttg cgctatagca ggcttgtttt
    12181 acttcaaagg aacgtgcaaa aggctggata ccaactaagt gatgtacata gatagctgat
    12241 gcctttgact gatcagtact cgattcagtt gccaaaataa ccatatcaat cttagctcga
    12301 tcttcgtcgg ttaagatttg atctgctgca gaagcagcta aagtaataat gtcttcagtg
    12361 actggtgcca cactaaaaga ctcaatgagt agcccttgac taaattttgc tggatccact
    12421 tggcgtgcaa gcgctaaatc ttccaatttc aagacatatt gactggttgc aaaaccaatc
    12481 ttatcaattc caattgtcat atttgtcctt cttttagtta tttttatagt cttacccatt
    12541 ctatcacatt ttactgcaaa attagactga taaagttatt tgttttatcc tttaatagtt
    12601 aaaatgttca tgaccacatg aagtttttcg aatgtttatt tattctggta ttttttggta
    12661 aaattagtta gaatagtatg aagtatgctt acagaaaggt tattagagat gacaaaagct
    12721 gaccagattt ttaaagctaa tattcaaaaa attataaacg aaggatcatt gagtgagcaa
    12781 gcacgtccta aatataaaga tggtcgaaca gctcattcta agtatattac tggggcgttt
    12841 gctgaatatg atttagctaa aggagagttt cccatcacaa ctttacgacc cattccaatt
    12901 aaatctgcta taaaggaatt attttggata tatcaagatc agtctaacag tttagatgtg
    12961 ctagaagcta aatataatgt tcattattgg aatgaatggg aagttgacca gacacgtact
    13021 attggacaac gttatggtgc tgtagtcaaa aaacatgata tcatctcaaa aatactaaag
    13081 caattagcag aaaatccttg gaatcgtcgt aatgtcattt ctctctggga ctatgaagca
    13141 tttgaggaga caaaagggtt gttgccatgt gcctttcaaa tcatgtttga tgttagacgt
    13201 gtaggggaag atctttattt agatgctagt ttgacgcaac gttctaatga catattagtt
    13261 gctcatcaca tcaatgccat gcaatacgtt gccttgcaga tgatgattgc taaacatttt
    13321 ggatggaaaa taggtaagtt cttttatttt gttaacaatt tacatattta tgacaaccaa
    13381 tttgatcagg ctcaagaatt actaaaacga cagccagttg ccagtcagcc taagcttgtt
    13441 ttaaatgttc cagatgggac taatttcttt gatattaaac cagatgattt tgaattacaa
    13501 aattatgatc cagtgaagcc tcagttgcac tttgaccttg ctatttaaac tttatttaga
    13561 ttttttgtga gcattttgtt cataaatttg gtaaaataaa tagagtttaa aatgaaggaa
    13621 ataattaatg acaaaggaaa ttattgctat ttgggcagag gatgaagcag ggcttattgg
    13681 tgttgcgggt aagttaccat ggtatttacc aaaagagtta gaacatttta aaaagacaac
    13741 gctacatcaa gcaatcttaa tgggccgtgt gacttttgaa ggaatgaatt gcaaaagact
    13801 tcctcaacgt caaacccttg tcatgacaag aaatagagat tatcaagtag atgaggtgtt
    13861 gacaatgact agtattgaaa aagtcttgga gtggtaccat gcgcaggata aaacacttta
    13921 tatcattggt gggaataagg ttttagaagc gtttaatggt tatttcgatc gtatcataaa
    13981 aacagttatc catcatcgtt ttaaaggaga tacctaccga ccaaatctag attttagtca
    14041 tttcacgcaa gaaagccaaa cgttctatgc tcgagacgct aaaaatcctt atgattttac
    14101 cgttactgct ttaaaacaca aatagtctga ggagatgata agcgatgcaa cgtagtattt
    14161 ttggagtatt tacggctttt ttgtgcgtga tttgtgttct ttgtgccatc ccagctttta
    14221 aaaagaatcg ttatggctta gcaagtctgt tcttattaaa tgcgtttact aatctggtta
    14281 atagtattca cgccttctat ggtacattgt tttgagatgc gacttgcaaa gacagcactg
    14341 gtgaagattt agctatcaag caagaatcac caaaaaccaa tacaacacat tgactaatat
    14401 taaaattcag taaaagataa gagaaagtaa gagaattata agaaatggca ggaagtagaa
    14461 ctaacgatat taaagtatac tgttcatttt gtggtaaaag ccaggatgac gttaaaaaaa
    14521 ttatcgcagg aaataatgtt ttcatctgca atgaatgcgt agctttatca caagaaatta
    14581 taaaagaaga attggctgaa gaagtccttg ccgatttgac agaagttcca aaaccaaaag
    14641 agctccttga tgttttaaat caatatgtgg ttggtcaaga tcgtgctaaa cgtgctttat
    14701 cagttgctgt gtacaatcat tataagcgtg tctcgtttac agaaagtcgt gatgacgatg
    14761 atgtggattt acaaaagtcc aacattttga tgattggacc aacaggttca ggaaaaacct
    14821 tcttagcaca aactctggct aaaagcctca atgtaccatt tgctattgca gatgcaactt
    14881 ctttgactga agcagggtat gttggagaag atgtggaaaa tattttactc aaactgattc
    14941 aggctgctga ctataatgtc gaacgagcag agcgtggtat tatttatgtt gatgaaatag
    15001 ataagattgc taaaaaaggt gagaacgtat ctatcacacg tgatgtatct ggtgaaggtg
    15061 tacagcaagc ccttcttaaa attattgaag gtacggtagc aagtgttccc ccacaaggtg
    15121 ggcggaagca ccctaatcaa gaaatgatcc aaattgatac aaaaaatatc ttgtttattg
    15181 ttggcggggc ttttgatggc atcgaggaga ttgttaaaca acgtttaggt gaaaaagtta
    15241 ttggttttgg tcaaaacagc cgtaaaattg atgacaatgc ttcttatatg caagaaatta
    15301 tttcagaaga tattcaaaag tttgggctta ttccagaatt tattggccgc ttaccagttg
    15361 tggcagccct tgaacaacta aacacatcag atttgattca aattttgaca gaaccaagaa
    15421 atgccctggt caagcaatat caggcccttc tttcttacga tggcgtcgaa ttggcatttg
    15481 ataaggaagc cttagaagcc attgccaata aagccattga acgtaaaaca ggagcacgtg
    15541 gtcttcgttc tattattgaa gaaacgatgt tagacattat gtttgaaatt ccaagccaag
    15601 aagatgtcac aaaagtgcgt atcaccaagg ctgctgtcga aggcaagtca aaacccgttt
    15661 tagagacagc ttaaggagaa ataatggctg aagaacaagt cttaaatacc cataacgcct
    15721 caattttatt gagcgctgcc aataaatctc attaccccca agatgatttg ccagaaattg
    15781 cccttgctgg acgttcaaat gttggtaaat caagttttat taataccata ttggggcgca
    15841 aaaatttggc aagaacctca agcaagccag gaaaaaccca actattaaat tttttcaata
    15901 ttgataacaa gcttagattt gtggatgtcc ctggttatgg ctatgccaag gtttctaaaa
    15961 gtgaacgggc caaatgggga aagatgatcg aagaatactt aaccagcaga gacaatctgc
    16021 gtgcagtcgt gagtttggtt gatttacgtc atgccccttc aaaagaagat attcagatgt
    16081 atgacttttt gaagtactat gacattcctg ttattgttgt ggcaaccaaa gcagataaga
    16141 ttcctcgtgg caaatggaac aaacatgagt ctgtggtcaa gaaagctttg aattttgaca
    16201 aatctgatac ttttatagta ttttcttcgg tagaacgtat tggtatcgat gattcttggg
    16261 atgctatttt agagcaggtc tagcgatagg tgttgattta tctatgctaa aacattctca
    16321 gtagagggtg tttttcttat cgaaaacata aaaaatcata gttaggttta ccgattttac
    16381 ttgacgcctt aatgatgaca tgctagtatt ttatattaag gagatgagtc atgacacaag
    16441 tagcccaagg gcgctctaaa ggaaagtcat tgttatgcca tcaatgcaat gccctttttg
    16501 tatttattat ttttcttatc ttgcttgctg tactttttgg tgttcgatat cgtaatagct
    16561 ctattgaagg tatctggcga acaacaagca ttgatcagaa gttaggcgat gattttgcta
    16621 aacgtcttac tggtttgcat cagagccctc ttattgatga tagtttatta acgtcatctc
    16681 agatgatact aacagttaaa aataacaacg ttgacttgag ttttagtgtt caagttgaac
    16741 gagacatttt tgtcaaacgt ttagctgctt atcaccaaaa tgaattatta aagacattaa
    16801 aggaaaatca tttagttgtt ggtgacttga gtagtaaaga acgccaaatt attgagaata
    16861 gtatgccagc tagccatgaa ttagaaatga ttttagatca agcttttgag aaattagctt
    16921 ctcagatcgg aggcaaatat aatcaaaaaa caggacattt atctgctgtt gttctcaaag
    16981 gaaaagttaa tcgtattttg cacaccattg atatcaaaga agaagtagca gcgggacata
    17041 cttctttttc aaaaggattg ctcacaccta atggttattt tgattacacc agatttggaa
    17101 agaaacttga gttattaggc gatgaaaaaa ttatttttaa aaaagcgcta aaaaaaagtc
    17161 cgtcaagtgt ataacgtagt cacaagacgg attttttcat gttttgaaaa ttattcagtt
    17221 gaagaagttt attactgatc gttttgccta atcactagtt gtccttcttt taggattgcc
    17281 tgaagttttt tgacttctgg atggtcaaga taataatcag ttatacggtc acgaatttcc
    17341 tgttcgataa tacgtcgtaa tggacgagct cccatagcgc gattgtaccc taaatctatc
    17401 aaatgagctt tagcgtcatc agcaatcgtt aaactaatac ctttttttgc ggtggtttga
    17461 tttacttcag ctagcattaa atcaacaatt tcttctaaat tttctttttg cagatgatta
    17521 aatttaatga tactattaaa acggttcaaa aactctggtc tgaaataagg tgcaatgcga
    17581 tccataatgt taaacccaga agtttccgta tcttgttggc caaaaccggc atttgatgta
    17641 gcaataataa cggtattttt aaagttaata gtatttcctt gtccatctgt caatcgtcca
    17701 tcatctaaca cttgtagaag aagtgtaatg atttgaggat cagctttttc aatttcatct
    17761 agtaagacaa tagcataagg gttacgacga acacgctcag ttagggtatt attattatca
    17821 tcgtagccaa cataaccagc cgttgtccca atcaattttg acacagcagt gcgatcgtta
    17881 tattcggaca tatcaagtcg aataatagca tctttggaac caaataaatc aagtgctaat
    17941 tgtttagcta actctgtttt accaacacca gttggaccca caaaaaggaa agaacctatt
    18001 ggacggttac catcatcaaa acctgctcga tttcgtcgaa tagcgcgtgc tactgctgca
    18061 acagcctcat cttgtccgat gacatggctt tttaagcggc tgctaattcc tttgagcctt
    18121 tctaaatcat tagtccccat gttagagaca ggaataccag ttaagcgctc tacagactct
    18181 gcgatatcat tgactgtagc agtgacattt tgggtttggt tatgttgctc aatttgcttt
    18241 tgtaatgttt caatccgtgt ttttgctgct aaagcttttt caaagtcttc tttggcgaca
    18301 gctttttctt gcctctcttt ttgtttagct atttcagttt cgagtgtttt taagtcagtg
    18361 accgggtgtt gcgcagctaa atgagcagct gtcatatcca gtaagtcaat tgctttatct
    18421 ggcaggctac gctgtgggat gtattgaata gaataatcaa cagcagcttt tagaacatta
    18481 tcaggaaggg taatatggtg gtgttgttca taaagattac gaatacccat taaaatgtga
    18541 aaagtatctt cagctgatgg tgcattcact ttaacttcat taaagcgacg agcgagagca
    18601 gcatttttta aaatagtatt gcggtattcg tcttgagtag tagcaccaat cagtgttaat
    18661 tctcctcgtg acaaggctgg tttaagaata tctgcaagtc cctttgagcc tgaatcgctg
    18721 cttgtggcac cagcgccaac tatttgatga atttcatcaa aaaataagat gatattccca
    18781 gcttctttta cttcttgaat caagttttga atggtctctt caaaggaacc acgatactgt
    18841 gtaccagctt caagactaga aatatcaatt gatacgattt ccttgttttt aatagcagct
    18901 gggacatctc cattaacaat ggcttgagct aaaccttcaa tgactgcagt tttaccgaca
    18961 ccagcatctc cgacaaggac aggattattt ttagttcgtc gtgccaagat ttcagcggta
    19021 tcttgaattt ctttgttacg accgatgacg gggtcaagat gcccttgacg agcttcttgt
    19081 gttaagttag ttccgagttg agtcaataca ctatcagctt taggtgttaa catttgagaa
    19141 tttgttgctt tggttgtcgc tacagggagt tgtcctgttt gacgatagtg ttggaattct
    19201 tcgggagtta tttcgcgacc gttaacgaga tagcggggat tttcgctacg ataaccaccc
    19261 atgtttgcca ttaattggtt gaaaatatca tccatattta caaaaggatc tttaccagaa
    19321 aaatgtgtca tatcagttac ctcgcaataa aaaatagact ataagatgtt tgtaagcagt
    19381 cgcagaatcg ttgttaggat ttcacatgat aggaaaccct ttgctaataa aatgattcag
    19441 acattatttt tactcatctt atagtattat tataatcaaa aggtcagtaa aggtcaaaca
    19501 aaaacacttg attttcaaaa agctaaataa acacgaaaag taagtgctta tatgatgact
    19561 ttagaataaa tcataagttt taaaaagatc atcctattct atagcactta ggctgtcaat
    19621 gagagaaaaa caaatgtttt cattaacaag ttggcgcttt catggtataa taaagtgcta
    19681 agacgcaatt ctattataag ggggaatcat ggaagcactc aaaaaaattg ccggtgttac
    19741 cgctgcgcaa tatgtgactg atggcatgac cattgggctt ggaactgggt caacggctta
    19801 ctattttgtt gaggaaattg gccgtcgcgt aaagcaagaa ggactgcaag tggttggtgt
    19861 gactacgtca agtgtgacta gcaagcaagc agaaggctta ggcattcctt taaaatcgat
    19921 agatgatatt gatagcattg acctcaccgt agatggtgct gatgaagtgg ataaggattt
    19981 caatggaatc aagggtggcg gcgcagcctt actaatggaa aaaattgttg caacaccaac
    20041 aaaagaatac atctgggttg ttgatgcttc taaaatggta gagcatctgg gtgcctttaa
    20101 gttaccagtc gaagtcgttc aatatggtgc tgatcgtttg ttccgtgtct ttgaaaaagc
    20161 tggttacaaa ccgtcttttc gtatgaaagg tgatagtcgt ttggtgacag acatgcaaaa
    20221 ctatattatt gatcttgatt tgggttgtat taaagatcct gttgcttttg gtcacctgct
    20281 agatggaaca gttggtgttg tggaacacgg tctctttaat ggtatggttg ataaggtgat
    20341 tgttgctagt aaagacggcg ttactgtttt agaagcgcct aaagcaggct agtcttagca
    20401 tataacagtg ttaatatatt ttataggtat tggggagtca accgtaacct cccctagatt
    20461 ggagacttat gtcaaaattt aatcgtattc acttggttgt tttagactct gtcggtattg
    20521 gtgcagcgcc agatgctgac aaattcttta atgctggtgt agctgataca gattctgaca
    20581 ccctcggtca tatttcagaa acagcaggct tatcggttcc aaatatggca aaaattggac
    20641 ttggaaacat ttctcgtccg ataccactaa aaactattcc aacagaagac aatcctactg
    20701 gttacgtgac caaattggaa gaagtatctc ttggaaaaga taccatgaca ggccattggg
    20761 aaattatggg cttgaacatt acggaacctt ttgacacttt ctggaatggt ttcccagaag
    20821 agattttaac taagattgaa gaattctcag gtcgcaaaat tattcgtgaa gctaataaac
    20881 cctattcagg aactgcggtt attgatgatt ttggtccgcg tcaaatggaa acaggtgagt
    20941 tgattgttta cacctcagca gacccagtgc ttcaaattgc agcccatgaa gacattattc
    21001 ctgtggaaga gctttacaag atttgtgaat atgctcgctc catcacgctt gaacgccctg
    21061 cccttttagg ccgtattatt gcgcgtcctt atgtaggcga accaggaaac tttacacgta
    21121 cggctaaccg tcatgactat gcagtgtctc ctttccaaga taccgttttg aacaaactgg
    21181 ctgacgcagg cgttccaact tatgccgttg gtaagattaa tgacatcttt aatggttcag
    21241 gtattacaaa cgacatggga cacaataagt cgaacagcca tggtattgac acgcttatta
    21301 agactcttca attacctgag tttactaagg gattctcctt tactaacctc gttgattttg
    21361 atgccaactt tggtcatcgt cgtgacccag agggttatcg tgactgcttg catgaatttg
    21421 acaatcgcct acctgaaatc ttcgcaaata tgaaagaaga cgatttgctt ctcatcactg
    21481 ctgaccatgg taatgaccca acctatgcag gcaccgacca cactcgtgaa tacattccat
    21541 tgcttgccta ctctgcatcc tttactggaa atggtcttat tccacaaggg cattttgcgg
    21601 atatttcagc aactgttgca gaaaattttg gagttgatac agccatgatt ggggagtcat
    21661 tccttggtca cttaaaataa gaaagttagt aattgataat ggaagaaatc acgatttatc
    21721 ataatccaaa ctgtggcact tcacgtaatg ttcttgccat gattcgccat gcaggtattg
    21781 agcctactat tattgagtat ttgcagacgc cacctaatcg ggagacttta attgagctct
    21841 tacaatcgat gggaatctct gcgcgagaac tcttgcgaac caatgttcct gaatttgagg
    21901 ctcatggatt agcaaatcaa gcagtagctg agaaagacgt tattaatgcc atgttggcgg
    21961 atccaatttt gatcaatcgt cctattgttg tgactcgcaa aggagtaaaa ctttgccgtc
    22021 cttctgagac cttactggat attcttcctg ttcccctgcc tagtccttat atgaaagagg
    22081 atggcgagag tgtgaaccct attgaggtaa aaggagaaaa agatgtcatt gatgactaaa
    22141 attaacgaaa caaaagactt tttagttact aaaggaattg aaactcccga atttggatta
    22201 attctgggtt caggccttgg tgaacttgct gaagaagtag aaaatgctat tgttattgat
    22261 tatgcagata ttccaaactg gggcaagtca acggttgttg gccatgctgg aaagttagtt
    22321 tacggagatt tagctggccg taaagtgctc gccttacaag gacgtttcca tttttacgaa
    22381 ggaaatccgc ttgaagtggt gactttccct gttcgtgtca tgaaagctct tggatgtgaa
    22441 ggcgttcttg tcacaaatgc agctggtggt atcggctacg gcccaggtac cttgatggca
    22501 atcacagacc atatcaatat gaccggaaat aacccactaa ttggtgaaaa cttggacgag
    22561 tttggtccac gcttccctga tatgtctgat acctatacta aggtttatcg cgataaggca
    22621 catgaagtgg ctgaaaaaat gaacatcaaa ctagaagatg gtgtttatat gggcttgact
    22681 ggtccaactt atgaaacacc agcagaaatc cgtgctttca aagtattagg agcagatgct
    22741 gttggcatgt caactgtacc agaagttatt gtggctgctc attctggact taaagtgctt
    22801 ggcatttcag ctattacaaa ctttgcagct ggttttcaat cagaattgaa ccatgaagaa
    22861 gttgtggaag tgacccaaca tatcaaagaa gatttcaaag gtcttgttaa agctatttta
    22921 gcggagttgt aggacgaaca aaatcaactg gagcattatt acaggaatgt tattttatgt
    22981 cgtcagtttg gcacatctgt tcgcaataaa aaaatcaaaa aataatcacg gagtgtgagt
    23041 tccatgaatg ttgaactcag attctagctt ttctaactaa cataaccaac gaagggaaag
    23101 agacgtattc ggtttaaatt aagcatggac tcgaacattt cttcgtatta atttttaaat
    23161 aataaaggaa agagagttag tcctttctta tttgaactcg atttttagct ctcttcttct
    23221 attcaatatc aattatgaaa ggaatggggt tgtattcaat tgaatacggg cggaaagctc
    23281 ggaaactaga taaactaact tttgtgctca cacatggcgt tagttcccta aatttcagtt
    23341 gctttctgtc cgccctctga ccttctacaa aataaaagga aaggtagggc taactgtatt
    23401 cattgaattg aatacgggcg gagaactgtt tttcttactt ataaaaaata aaaaaataag
    23461 aaaggtgagg gctgagttca ttaatttgaa ttcgggctaa ggacttggtc aaaaagataa
    23521 gttgtctaga aaatcaggat tttctgccaa cttcctattt tgccatcgtt cttttaacgc
    23581 cctcagtatc ataatttatg tctatccata tttctgccaa aaaaggcgat attgctgata
    23641 aaattcttct acctggagac cctttgcgcg cgaaatttat cgctgaaaat ttcctagaag
    23701 atgcagtgtg ttttaacgaa gtgcgtaaca tgtttggcta cacaggaact tacaaagggc
    23761 accgcgtttc tgtcatggga actggtatgg ggatgccttc gatctctatc tatgctcgtg
    23821 aattaatcgt tgattacggt gtcaaaacat tgatccgtgt tggtactgcg ggtgcgattg
    23881 acccagaagt tcatgtgcgt gaattggtgc ttgcccaagc agcagcaacc aactcaaaca
    23941 ttatccgtaa tgacttccca gaatttgatt tcccacaaat tgctgatttt ggtttacttg
    24001 acaaggctta ccatatcgcc agagaaatgg gagtaacaac tcacgttggg aatgtgcttt
    24061 cttctgatgt cttttacact aacatgcctg agcgtaatat ggctcttggt aaattgggtg
    24121 ttaaagccat cgaaatggaa gcagcagctc tttactattt agctgctcag caccatgtca
    24181 aagctcttgg tattatgaca atttctgaca atttgaacga cccaacagaa gacaccactg
    24241 ctgaagagcg ccaaacaact ttcactgata tgatgaaggt cggcttggaa accttaattg
    24301 ccaatgacta ataatcaaac gctagacatc cttttggatg tctatgctta taatcacgcc
    24361 tttagaattg ctaaagcctt gccaaatatc cctaaaactg ctctctattt actagagatg
    24421 ttaaaagagc gcagagaatt gaaccttgcc tttctggcgg aacatgcagc agagaatcgg
    24481 accattgaag accagtacca ctgttcatta tggattaacc aatcgcttga agatgagcag
    24541 attgccaatt acattttgga tttagaagtt aaagtaaaaa acggtgctat tattgatttc
    24601 gtcaggtcag tgtcgcctat tctttaccga ctttttctca gactaatcac gtcagaaatt
    24661 ccaaacttca aggcttatat ttttgataca aagaatgacc aatatgatac ctggcagttt
    24721 caggccatgt tggaatctga tcacgaggtt ttcaaggctt acctgtctca aaagcagtct
    24781 cgcaatgtga cgaccaaaag cttagcagac atgttgacgt tgacctcctt acctcaggaa
    24841 atcaaggact tggttttttt gttacgacat tttgaaaagg ctgtccgtaa tcctctggct
    24901 catttgatta agccttttga tgaagaggaa ctgcatcgca ccactcattt ttcttctcag
    24961 gcttttttgg aaaacattat caccttagcg actttttctg gtgtaatcta ccg
//
LOCUS       DAUCLR010000023        24704 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.023, whole genome shotgun sequence.
ACCESSION   DAUCLR010000023 DAUCLR010000000
VERSION     DAUCLR010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24704
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.023"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            39..1565
                     /gene="glpK"
                     /locus_tag="VGT87_000726"
     CDS             39..1565
                     /gene="glpK"
                     /locus_tag="VGT87_000726"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEQ3813603.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNQKGEKVSSSQKEFPQIFPHAG
                     WVEHNANQIWNSVQSVIAGAFIESSIKPSQIEAIGITNQRETTVVWDKKTGVPIYNAI
                     VWQSRQTAPIAEQLKQDGHTKMIHEKTGLVIDAYFSATKIRWILDHVPGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAVHVTDYSNAARTMLYNIKDLTWDDEILELLNIPKDMLPEV
                     KSNSEIYGKTAAFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GDEMQLSSNNLLTTIGYGINGKVHYALEGSIFIAGSAIQWLRDGLKMIETSPESEQFA
                     LASTSDDEVYVVPAFTGLGAPYWDSNARGSVFGLTRGTSKEDFVKATLQSIAYQVRDV
                     INTMQVDSGIDIQQLRVDGGAAMNNMLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     AVGYWEDMDALKELNATGQLFKASMNESRKEKLYKGWKRAVKATQVFTQEEDADDDTK
                     "
     gene            1581..3410
                     /gene="glpO"
                     /locus_tag="VGT87_000727"
     CDS             1581..3410
                     /gene="glpO"
                     /locus_tag="VGT87_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEQ3813604.1"
                     /translation="MEFSKEIRRLALQKMQERDLDLLIIGGGITGAGVALQAAASGLD
                     TGLIEMQDFAQGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEPGSTFSMFRLKVAMDLYDLLAGVSNTPAANKVLTKEEVLKREPDLKQEGL
                     LGGGVYLDFRNNDARLVIENIKRANRDGALIASHVKAEDFLLDDNGKIIGVKARDLLS
                     DQEIIIKAKLVINTTGPWSDEIRQFSHKGQPIHQMRPTKGVHLVVDRQKLPVSQPVYV
                     DTGLNDGRMVFVLPREEKTYFGTTDTDYTGDLEHPQVTQEDVDYLLGVVNNRFPNANV
                     TIDDIESSWAGLRPLLSGNSASDYNGGNSGKVSDDSFDHLVDTVKAYINHEDSREAVE
                     KAIKQVETSTSEKELDPSAVSRGSSFDRDENGLFTLAGGKITDYRKMAEGALTGIIQT
                     LKEEFGKSFKLINSKTYPVSGGEINPANVDSEIEAYAQLGTLSGLSMDDARYLANLYG
                     SNAPKVFALTRQLTAAEGLSLAETLSLHYAMDYEMALKPTDYFLRRTNHLLFMRDSLD
                     ALIDPVIKEMAKHFEWSDQERVAQEDDLRRVIADNDLSALKGQ"
     gene            3421..4122
                     /locus_tag="VGT87_000728"
     CDS             3421..4122
                     /locus_tag="VGT87_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEQ3813605.1"
                     /translation="MDIFGEFLGTALLVLLGNGVVAGVVLPKTKTHASGWIVIATGWG
                     IAVAVAVFISGKVAPAHLNPAVSLAFAMSGTIAWSTAIAYSLAQLLGAMVGSTLVFLQ
                     FRPHYLAAESQADILGTFATGPAIRDTSSNLLSEIFGTFVLMLGILAFGLYDMPAGLG
                     TLCVGTLVIGIGLSLGGTTGYAINPARDLGPRLVHAILPLNNKGDSDWSYAWIPVVGP
                     IIGAALAVLLFQVMS"
     gene            4231..5577
                     /locus_tag="VGT87_000729"
     CDS             4231..5577
                     /locus_tag="VGT87_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEQ3813606.1"
                     /translation="MTLTTKTIHVIGASFAGLAFVDKYKDLNPDSQIILIDKESCPNY
                     IPNGINQLFRGDIQDLSDAMWGRACLAAQIESNYRFIQAEVLAIEAPSNTLLLKDSQG
                     RVFEEGYETLVCAMGASPQSHYIETSQTNKVLVTKYYEESQASLKLIEASQEVLVIGA
                     GLIGLDLAYSLSLQGKRVKLIEAAERPDFYQTDAELIAPVMAEMSTHHVTFINNKRVT
                     AIHEIEGKVVAHTEQGDTFQGDLAILAINFRPNTHLLQGQVACALDKTILVNENLQTS
                     QANIYAIGDMVSLHFGILGMDYYTPLINQAMKTGQALALHLAGYPIPPLQTVKVLGSS
                     HFGYYRASVGVTEEEAELYMDTCSYLYQNGNSKNLFWLKLIARKTDGILIGAQLLSKT
                     NALVIANQLGQALALKVTDGDLAFQDFLFLQGHSDLAYHLHEACLKLFEKRLRHED"
     gene            5567..7066
                     /locus_tag="VGT87_000730"
     CDS             5567..7066
                     /locus_tag="VGT87_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ3813607.1"
                     /translation="MKIEDLMDKERKAQYRLLVTLYHAKETLRLKDLMRLSNLSKVTL
                     LKYIDNLNHLCREQGLACQLLLEKDSLSLKENGQFHWEDLVALLLKESVAYQILTYMY
                     CHEHFNITNLSVELMVSEATLNRQLAHLNQLLSEFDLALSQGRQLGSELQWRYFYFEL
                     FRHTLTRQGIDALVNQLDASHLATLIERLIGQSLSAEALEQLLIWLAISQARMSFQKG
                     YNDHFLRDSDFMTSNIFFKRLESMLLHYLRRYALEFDAFEAKSLFVFLHAYPLLPIAS
                     MKYSLGFGGPIADHISEALWLLKKAHVIIHQTKEEIIYGLGIFFSKAYFFKGAILSQP
                     TNSQYLYQLVGEDKRALLRVIINHLVLQMGQETDFSQQLSDDILVLLIFSIERHHEPL
                     LVGLALGQNKVEAAIAELAIRRHLGHRRDFQLMPYDHQKVYDCLITYQTVCLPRQDLP
                     YYRLKQYSSPYELTALEAFLKDLFQQKNTREEELSLSPAAKSSFAHKTV"
     gene            7192..7836
                     /gene="fsa"
                     /locus_tag="VGT87_000731"
     CDS             7192..7836
                     /gene="fsa"
                     /locus_tag="VGT87_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEQ3813608.1"
                     /translation="MKFFLDTANVAAIKAINELGVVDGVTTNPTIISREGRDFETVIK
                     EICDIVDGPISAEVTGLTADAMVEEARSIAKWHDNVVVKIPMTTEGLKATNILSKEGI
                     KTNVTLIFTVSQGLMAMKAGATYISPFIGRLEDIGTDAYQLISDLREIIDLYDFQAEI
                     IAASIRTTAHVEAVAKLGAHIATIPDPLFAKMTQHPLTTNGLKTFMEDWASFKK"
     gene            8055..10040
                     /gene="tkt"
                     /locus_tag="VGT87_000732"
     CDS             8055..10040
                     /gene="tkt"
                     /locus_tag="VGT87_000732"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEQ3813609.1"
                     /translation="MTFDAIDQLAVNTVRTLSMDAIQAANSGHPGLPMGAAPMAYVLW
                     NHFMNINPKTSRNWSNRDRFILSAGHGSAMLYSLLHLAGYDLSVEDLKNFRQWGSKTP
                     GHPEVNHTDGVEATTGPLGQGIANAVGMAMAEAHLAAKFNKPGFDIVDHYTFALNGDG
                     DLMEGVSQEAASMAGHLKLGKLVLLYDSNDISLDGPTSMAFTEDVKGRFEAYGWQHIL
                     VKDGNDLEEIAAAIEAAKAETEKPTIIEVKTIIGFGAEKQGTSAVHGAPLGAEGIAFA
                     KKAYQWTHQDFEVPAEVTERFAQGLQARGEKAEQAWNDLFAAYEAEYPELAAEYQKAF
                     TNEAAQVELEAHELGSSMASRVSSQQAIQQISEQVASFWGGSADLSASNNTMVKAETD
                     FQPGHYEGRNIWFGVREFAMAAAMNGIALHGGTRVYGGTFFVFSNYLLPAVRMAALQN
                     LPTVYVMTHDSIAVGEDGPTHEPIEQLASVRSMPNLNVIRPADGNETNTAWKRAIAET
                     DRPTMLVLTRQNLPVLEGTKELAEDGLNKGAYILSEAKGDLDGIIIATGSEVKLAMDT
                     QEALEAEGIHVRVVSMPSQNIFDEQSAEYKESILPAAVTKRLAIEAGSSFGWAKYVGL
                     AGKTLTIDTWGASAPGNRIFEEYGFTVANATELYKSL"
     gene            10232..10498
                     /locus_tag="VGT87_000733"
     CDS             10232..10498
                     /locus_tag="VGT87_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEQ3813610.1"
                     /translation="MEKKRQRLYDVIRHAYDYPENRENVALSQLLLAASNRLIKHSNP
                     LLVAYQLNQDVDNYLLDNDILLPKSLCRFKQSLEKYILEEASSV"
     gene            10532..11266
                     /locus_tag="VGT87_000734"
     CDS             10532..11266
                     /locus_tag="VGT87_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ3813611.1"
                     /translation="MIEFKHVSKLYGDKEALSDLNVTINDGEIFGLIGHNGAGKTTTI
                     SILTSIIEASYGEVFVDGQLLTENREAIKKQIAYVPDSPDIFLNLTPNEYWQFLAKIY
                     GVSDEDREERLAQLTTLFELKEEVNQTIDSFSHGMRQKVIVIGALVSNPNIWILDEPL
                     TGLDPQASFDLKEMMKAHAASGHTVLFSTHVLSVAEQLCDRIGILKKGKLIFVGTIDE
                     LKEHHPDKDLESIYLELAGRKAQEEG"
     gene            11271..12899
                     /locus_tag="VGT87_000735"
     CDS             11271..12899
                     /locus_tag="VGT87_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ3813612.1"
                     /translation="MNWSTIWELIKINILYSNPQSLANLKKRQEKHPKENFKAYKSMM
                     RQQALMIAMFLVIYLFMFIGVDFSHYPGLFSFDVAMFFIMSTLTAFSSLYTIFYESND
                     LKLYIHLPVTSEELYIAKIVSSLGMGAVFLMPLISLLLIAYWQLLGNPLSILVAIVLF
                     LVLLVSSMVLAIYINAWVGKIIVRSRKRKLISTIMMFVSTFGAFVLIFAINISNNKRT
                     MTDGVFTDYPTIPYFKGFYDVVQAPFSTVALLNFWFPLLLILAMVYGIVTKVMPTYYR
                     EVFYISNENKVKQTKKPVNRPHQNQSLAQLLRKHHLLTLQNATLLTQTYLMPLMYVML
                     FIGPSLSRGTGFFKHISPDYFGVALLFGVSLGVMCATPTSFIGVGISLEKDNFTFIKS
                     LPITLKKFLLDKFCLLVGLQLIVPMVIYLVFGLFVLHLHPLLTIAFCLGYALSLIVQG
                     ELMYRRDYRLLDIKWQDMTQLFTRGDGQWLTMGLIFGNLIVAGVLGFGAVIIANIIQQ
                     PLLISILLSCLILMVLGLAQLWIQKTFWKSLERL"
     gene            12964..13785
                     /gene="proB"
                     /locus_tag="VGT87_000736"
     CDS             12964..13785
                     /gene="proB"
                     /locus_tag="VGT87_000736"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEQ3813613.1"
                     /translation="MMKRQFEDVTRIVIKIGTSSLVLPTGKINLEKIDQLAFVISSLM
                     NKGKEVILVSSGAMGFGLDILKMEKRPTNLAKQQAVSSVGQVAMMSLYSQIFAHYQTN
                     VSQILLTRDVVVFPESLANVTNAFESLISLGIVPIVNENDAVSVDEMDHATKFGDNDR
                     LSAVVAGITKADLLIMLSDIDGLFDKNPTIYEDAQLRSHVAVITQEIIASAGGAGSKF
                     GTGGMLSKIQSAQMVFENKGQMVLMNGANPRDILRVLEGQPLGTWFKQIEEVTHD"
     gene            13778..15028
                     /locus_tag="VGT87_000737"
     CDS             13778..15028
                     /locus_tag="VGT87_000737"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEQ3813614.1"
                     /translation="MTDMRRLGQRAKQASLLIAPLSTQIKNRFLSTLAKALVDDTQTL
                     LAANQKDLANAKEHGISDIMMDRLRLTSERIKAIAQGVQQVADLADPIGQVIKGYTNL
                     DGLKILQKRVPLGVIAMIFESRPNVSVDAFSLAFKTNNAIILRGGKDALHSNKALVKL
                     IRQSLEKSGITPDAVQLVEDPSHAVAEELMQATDYVDVLIPRGGARLIQTVKEKAKVP
                     VIETGVGNVHIYVDAQADLDMATKIVINAKTRRPSVCNAAEGLVIHEAVAARFIPMLE
                     KAINQVQPVEWRADDKALPLFEQAVPAKAEDFETEFLDYIMSVKVVSSLEEAISWINQ
                     HTSYHSEAIITRDIKAAETFQDLVDAAAVYVNASTRFTDGFVFGLGAEIGISTQKMHA
                     RGPMGLEALTSTKFYINGDGHIRE"
     gene            15048..15263
                     /locus_tag="VGT87_000738"
     CDS             15048..15263
                     /locus_tag="VGT87_000738"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813615.1"
                     /translation="MTNEREKEEAKVALEELLHQIQVIHLELKIVRNWGYFVFLETVC
                     LSAGSNVVSKVKQLLIDLQVTLQNCRR"
     gene            15306..15470
                     /locus_tag="VGT87_000739"
     CDS             15306..15470
                     /locus_tag="VGT87_000739"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813616.1"
                     /translation="MSWDKTFGIWLDNLLTDWCVIKEAKNLLQAVEILEAAAKSVLAK
                     LADSEQKEQD"
     gene            15494..16507
                     /gene="rsmH"
                     /locus_tag="VGT87_000740"
     CDS             15494..16507
                     /gene="rsmH"
                     /locus_tag="VGT87_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEQ3813617.1"
                     /translation="MKSFSLTFSFLNLLKYGTIKVMTKEFHHVTVLLHETVDMLDIKP
                     DGIYVDATLGGSGHSAYLLSKLGEEGHLYCFDQDQKAIDNAQVTLKSYIDKGQVTFIK
                     DNFRHLKARLTALGVDEIDGILYDLGVSSPQLDERERGFSYKQDAPLDMRMDRQSLLT
                     AYEVVNTYPFNDLVKIFFKYGEDKFSKQIARKIEQARAIKPIETTTELAELIKAAKPA
                     KELKKKGHPAKQIFQAIRIEVNDELGAADESIQDAMELLALDGRISVITFHSLEDRLT
                     KQLFKEASTVDVPKGLPLIPEDMKPKFELVSRKPILPSHSELTANKRAHSAKLRVAKK
                     IRK"
     gene            16512..16835
                     /gene="ftsL"
                     /locus_tag="VGT87_000741"
     CDS             16512..16835
                     /gene="ftsL"
                     /locus_tag="VGT87_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEQ3813618.1"
                     /translation="MTNEKRTQAVTNALQKRIKTFSRIEKAFYTAIIVTAITMAVSII
                     YLQSRKLQLQQEITSLNSHISDQKLELNNAKQEVNELSRRDRIIDIAGKAGLSNRNNN
                     IKKVE"
     gene            16839..19094
                     /locus_tag="VGT87_000742"
     CDS             16839..19094
                     /locus_tag="VGT87_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEQ3813619.1"
                     /translation="MKKWQKYVLDYVVRDRRTPVENRVRVGQNMMLLTIFIFFIFIIN
                     FMIIIGTDQKFGVSLSEGAKKVYQETVTIQAKRGTIYDRNGTAIAVDSTTYSIYAILD
                     KSFVSASDEKLYVQPSQYETVADILKKHLGMKKTDVIKQLKRKGLFQVSFGPSGSGIS
                     YSTMSTIQKAMEDAKIKGIAFTTSPGRMYPNGTFASEFIGLASLTEDKKTGVKSLVGK
                     TGLEASFDKILSGQDGVITYQKDRNGTTLLGTGKTVKKAIDGKDIYTTLSEPIQTFLE
                     TQMDVFQAKSNGQLASATLVNAKTGEILATTQRPTYNADTLKGLENTNYKWYSALHQG
                     NFEPGSTMKVMTLAAAIDDKVFNPNETFSNANGLTIADATIQDWSINEGISTGQYMNY
                     AQGFAFSSNVGMTKLEQKMGNAKWMNYLTKFRFGFPTRFGLKDEDAGIFPSDNIVTQA
                     MSAFGQGISVTQIQMLRAFTAISNNGEMLEPQFISQIYDPNTASFRTANKEIVGKPVS
                     KKAASETRQYMIGVGTDPEFGTLYSKTFGPIIKVGDLPVAVKSGTAQIGSEDGSGYQD
                     GGLTNYVYSVVAMVPADKPDFLMYVTMTKPQHFGPLFWQDVVNPVLEEAYLMQDTLTK
                     PVVSDANRQTTYKLPNFVGKNPGETSSELRRNLVQPVVLGTGSKIKKVSHQPGQTLTE
                     NQQVLILSDRFVEVPDMYGWTKSNVKTFAKWTGIDISFKGTDSGRVMKQSVDVGKSLK
                     KIKKMTITLGD"
     gene            19096..20106
                     /locus_tag="VGT87_000743"
     CDS             19096..20106
                     /locus_tag="VGT87_000743"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEQ3813620.1"
                     /translation="MFLTLIAAIISFMVSAFTMPYFIKFYQLKKIGGQQMHEDVKQHL
                     AKAGTPTMGGTVFLLVATAVSLLVSLFSIKNTQSLALISGILSIVVIYGIIGFLDDFL
                     KIFKQINEGLTAKQKLALQLVGGLMFYFLHVSPSGISSINVFGYQLPLGIFYLFFVLF
                     WVVGFSNAVNLTDGIDGLASISVVISLVTYGVIAYVQSQFDVLLLIGAMIGALLGFFC
                     FNHKPAKVFMGDVGSLALGAMLAAISIALRQEWTLLIIGIVYVLETSSVMLQVSYFKY
                     TKKKYGEGRRIFRMTPFHHHLELGGLSGKGKKWSEWQVDAFLWGVGSLASLLVLAILY
                     VF"
     gene            20264..21607
                     /locus_tag="VGT87_000744"
     CDS             20264..21607
                     /locus_tag="VGT87_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEQ3813621.1"
                     /translation="MSFKDYHFKQYVQQALEEIGFVNPTEVQKRLIPIVNSGRDLVGE
                     SKTGSGKTHTFLLPIFEKLDEAKAEVQVVITAPSRELATQIFDACKQIAKHSQEEIRL
                     ANYVGGTDKLRQIEKLKGGQPHIVIGTPGRIYDLVKSGDLAIHKATTFVVDEADMTMD
                     MGFLDTVDKIAASLPKSVQILVFSATIPQKLQPFLKKYLTNPVIEQIKTKTVIADTID
                     NWLVSTKGRDKNGQLLEILKTMQPYMAMLFVNTKERADDLHAFLTANGLKVAKIHGGI
                     PPRERKRIMNQVKKLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGMAGTAITLYQPSDDSDIKELEKMGIAFTPKVLKNGEFQDTYDRDRRQNREKAYQK
                     LDTEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKERRAENRAKGRAERKAKKQHF"
     gene            21802..22605
                     /locus_tag="VGT87_000745"
     CDS             21802..22605
                     /locus_tag="VGT87_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEQ3813622.1"
                     /translation="MTSVFLTSGWAFYDHLISPIPHGKLFSWHAVFDAIPNIIQRLPI
                     TLGLTLSGATFGLVLALIFALVKINKVKLLYPIQAIFVSFLRGTPILVQLMLTYYGIP
                     LFLKCLNQKYGFDWNVNAIPASIFAITAFAFNEAAYASETIRAAILSVDTGEIEAAKS
                     LGMTSVQVYRRVIIPNATVVAIPTLINGLIGLTKGTSLAFNAGIVEMFAQAQILGGSD
                     YRYFERYISVALVYWSISILMEQVGRLIENKMAIKAPEQARNEKLGELR"
     gene            22605..23348
                     /locus_tag="VGT87_000746"
     CDS             22605..23348
                     /locus_tag="VGT87_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEQ3813623.1"
                     /translation="MITIRNLSKTFSEQKVLDSLALDIEKGQVIALVGASGAGKSTFL
                     RSLNYLEKPDSGSISIGDFTVDFETITTEQVLILRRKLAMVFQEFNLFERRTALENVK
                     EGLKVVKKLSDQEATKLAQAELAKVGLADRKHHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSITDAAKSGQTMVLVSHDMNFVYQVADRVLFLDQGKIL
                     EQGTPEEVFRHPQKERTKEFFASYSKTYI"
     gene            23452..23676
                     /locus_tag="VGT87_000747"
     CDS             23452..23676
                     /locus_tag="VGT87_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEQ3813624.1"
                     /translation="MLVEIFSLYVKGLILSSLTMMLICLIWTLWRAKTKRDKTLIERQ
                     AFLYEMLMVAILTIPILSFAFMSILVVIKS"
     gene            23740..24657
                     /gene="trxB"
                     /locus_tag="VGT87_000748"
     CDS             23740..24657
                     /gene="trxB"
                     /locus_tag="VGT87_000748"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEQ3813625.1"
                     /translation="MYDTLIIGSGPAGMTAALYAARSNLSVAIIEQGAPGGQMNNTFD
                     IENYPGYDHISGPELAMKMYEPLEKFNVENIYGIVQKIENFGDYKCVSTEDASYEAKT
                     VIIATGAKYRVLGVPGEEYYTSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEAIYLT
                     QFAKKVTVVHRRDQLRAQKILQDRAFANDKVDFIWDSVVKEIKGNDIKVSNVLIENVK
                     TGQVTDHAFGGVFIYVGMIPVTGMVTDLEITDQEGWIITDDHMRTSIPGIFAIGDVRQ
                     KDLRQITTAVGDGAIAGQGVYHYLESFPS"
BASE COUNT         7517 a         4519 c         5354 g         7314 t
ORIGIN      
        1 atcaaatagg aaaacgctta cttaaaggag ggtaatgaat gtcgcaagaa aagtatatca
       61 tggctattga tcagggaaca acaagttcac gagcgattat tttcaatcaa aaaggggaaa
      121 aggttagcag tagccaaaaa gaattccctc agattttccc acacgctggt tgggtagagc
      181 acaatgctaa tcagatttgg aattcggttc agtctgttat tgcgggtgct tttattgagt
      241 ccagcatcaa accaagccaa attgaagcaa tcggaattac caaccaacgt gagaccacag
      301 tggtttggga caaaaaaacg ggtgtaccta tttataatgc tattgtgtgg caatcacgac
      361 agacggcacc aattgctgag caactcaaac aagatggcca tacaaaaatg atccatgaaa
      421 agacaggcct tgttattgat gcttactttt ctgcaaccaa gatccgttgg attttggacc
      481 atgttcctgg tgctcaagaa cgagctgaaa aaggcgagtt actttttgga accattgata
      541 cttggctggt ttggaaattg acagatggtg ctgttcatgt gacggattat tccaatgcgg
      601 ctcgtaccat gctctataat attaaagact taacatggga tgatgagatt ttagagcttt
      661 taaatattcc aaaagatatg cttccagagg ttaaatccaa ttctgaaatt tatggcaaaa
      721 cagctgcctt tcatttttat gggggcgaag tgcctatttc aggtatggcg ggtgaccaac
      781 aggcagcttt gtttggccaa ctagcttttg agcctggtat ggtgaaaaac acttatggca
      841 caggttcttt cattatcatg aatactggtg atgagatgca gttatcgagt aataatcttt
      901 tgaccaccat tggttacggg attaatggga aggttcacta tgccttggaa ggttccatct
      961 ttattgcagg ctctgccatt caatggttgc gagatggtct taaaatgatt gagacttcac
     1021 cagaatctga acagttcgct ctagcatcaa ctagtgacga tgaagtttat gttgtgcctg
     1081 cttttaccgg tttaggagct ccttactggg attctaatgc gcgtggatct gtctttggct
     1141 tgacacgtgg taccagcaaa gaagactttg tcaaagcaac cttacaatct atcgcctatc
     1201 aagtgcgaga tgttattaat accatgcagg tggattcagg tattgacatt caacagctcc
     1261 gtgtggatgg cggtgcagcc atgaataata tgctcatgca atttcaggct gatattttag
     1321 gaattgatat tgcccgtgct aaaaatttag aaacaacagc tctaggagca gctttcttag
     1381 caggacttgc tgtgggctac tgggaagaca tggatgcttt gaaagagtta aatgccacag
     1441 gccagctctt taaggctagc atgaatgaat cgcgcaaaga aaaattatac aagggttgga
     1501 agcgcgctgt taaagcaact caagttttca cccaagaaga agatgcagat gacgatacaa
     1561 aataactaga ggaggatgga atggaatttt caaaagagat aagacgctta gctcttcaaa
     1621 aaatgcaaga aagagattta gacctactaa ttattggggg aggtattacg ggtgctggtg
     1681 tggcacttca ggcggcagct agtggcctag atacgggtct gattgagatg caagattttg
     1741 cgcaaggaac ctctagccgt tcaaccaaat tggttcacgg ggggcttcgt tacttgaaac
     1801 aatttgatgt ggaggtggtt tcagatacgg tgtcagagcg ggctgtggtg caacaaattg
     1861 caccccacat tccaaaacca gaccctatgt tattacctgt ttatgacgaa cctggcagta
     1921 cctttagcat gttccgtttg aaggtcgcta tggatttgta tgatctttta gcaggcgtgt
     1981 ccaatacgcc agcggccaac aaggtgttaa ctaaagaaga agtcttaaaa cgagaaccag
     2041 acttaaaaca agaaggcttg cttggtggtg gggtttacct tgatttccgc aataatgacg
     2101 caaggcttgt tattgaaaat atcaaacgag ctaatcgtga tggggctctt attgctagtc
     2161 atgtgaaagc agaagatttc ttgctagatg acaatggtaa gattattggt gtgaaggcgc
     2221 gtgatttgct gtcagatcaa gaaattatta tcaaggctaa attagtcatc aacaccacag
     2281 gtccatggag tgatgagatt cgtcaattct ctcataaggg acaaccgatt catcaaatgc
     2341 gccctacaaa aggggtgcat ctggtagtgg accgtcaaaa attaccagtg tctcaacctg
     2401 tctatgttga cacagggtta aatgatggtc gtatggtctt tgtcttgcca cgtgaagaaa
     2461 aaacttattt tggaacaacg gacacggact acactggaga cttggagcac ccacaagtta
     2521 ctcaagaaga tgtggattat ttgttaggcg ttgtcaataa ccgctttcca aatgccaacg
     2581 tgaccattga tgatattgaa agcagttggg ctggccttcg ccccttgtta tcaggtaata
     2641 gtgcttctga ctacaatggt ggcaacagcg gtaaagtcag tgatgatagt tttgatcact
     2701 tggttgatac tgtcaaagcc tatattaacc acgaagatag ccgagaagct gttgaaaaag
     2761 ctattaagca ggttgaaacc agcacatctg aaaaagaatt ggatccgtct gcagtgtcac
     2821 gaggttcaag ttttgaccgt gatgagaatg gcctctttac cttggcaggt ggtaagatta
     2881 ccgactatcg caaaatggct gaaggagcat tgacagggat tattcaaacc ctcaaagaag
     2941 agtttggcaa atccttcaag cttatcaatt caaaaaccta tcctgtttca ggaggtgaaa
     3001 tcaatccagc caatgtagat tcagaaatag aagcctatgc tcaattagga actcttagtg
     3061 gtctttcgat ggatgatgct aggtatttgg caaaccttta tggttctaat gcgccaaaag
     3121 tctttgcctt aactcgtcaa ttaacagcag ctgaagggtt aagtttagct gaaaccttgt
     3181 ccttacatta tgcgatggat tatgaaatgg ctcttaaacc gacagattat ttcttgagaa
     3241 gaacaaatca cctcttattt atgcgagata gtctagatgc tttgattgac ccagtgatta
     3301 aagaaatggc taaacatttt gaatggtctg atcaggaaag agtggcacaa gaagacgatc
     3361 ttcgtcgcgt gattgcagac aatgatttga gtgccctaaa aggccagtag gagggttaat
     3421 atggatatct ttggtgaatt tttgggtaca gccttattgg ttttactagg aaatggtgtg
     3481 gtagcagggg ttgtcttgcc caaaacgaaa acccatgctt ctggctggat cgtgattgcg
     3541 actggctggg gaattgcagt tgctgtggca gtctttatca gtggcaaagt tgcccctgcc
     3601 cacctcaatc cagctgttag ccttgctttt gccatgagtg gaaccattgc ttggtcaaca
     3661 gccattgcct atagccttgc ccaactattg ggagctatgg ttggttcaac tctggtattc
     3721 cttcagttca ggccacatta tctggctgct gagagtcagg ctgatatttt agggacattt
     3781 gcgacaggtc ctgctattcg agatactagc tcaaacctat tgagtgaaat ctttgggacc
     3841 tttgtcttga tgcttggtat tttggcattt ggcttatatg atatgccagc aggactagga
     3901 accctctgtg taggtacctt ggttattggg attggattat ccttaggagg aacgacaggt
     3961 tacgccatta acccagcgcg tgatttagga cctcgtctag ttcatgctat tctaccactc
     4021 aacaacaagg gagattctga ttggtcttat gcttggatac ctgttgtagg accaattatt
     4081 ggagcggctt tagcagtttt gcttttccaa gtcatgtcct aaccatcact aaaggggttg
     4141 gctatcatag gccagctcct ttgctttttc tttataggat taggtaaaac gctttcatat
     4201 cttaaaaaca actgctataa taatactatt atgacattaa cgacaaagac catacatgtt
     4261 atcggggcat cctttgctgg tctagccttt gttgataaat ataaggacct taatccagat
     4321 tcccaaatca ttctcattga taaggaaagc tgtcctaatt atattccaaa tggtatcaac
     4381 caattattca gaggagacat tcaagacctg tctgatgcta tgtggggaag ggcctgccta
     4441 gcagcacaaa tagaatctaa ctaccggttt attcaagcag aagtgttagc tatcgaagct
     4501 ccctcaaaca ccttgcttct caaagacagt caaggacgag tttttgaaga aggttatgaa
     4561 acgcttgttt gtgccatggg tgctagcccc cagtctcatt atattgagac gtcacagacc
     4621 aacaaagttt tggtaaccaa atattatgaa gaaagccaag ctagtttaaa actgattgag
     4681 gctagccaag aggtcttggt tattggtgcc ggcttgattg gcttagacct tgcctacagt
     4741 ttgtccttac aaggcaaacg ggtcaaactg attgaagcag ccgaacgtcc agatttctat
     4801 cagactgacg cagaactgat agcccctgta atggcagaaa tgtccactca ccatgtcacc
     4861 tttattaata ataagcgtgt tacggctatt catgagatag agggaaaagt tgtcgctcat
     4921 accgagcaag gggacacctt ccaaggtgat ttggctattt tagccattaa ctttaggcca
     4981 aatacacacc ttttgcaagg acaagtggct tgtgctctgg ataagaccat tttggtcaat
     5041 gaaaacttac agaccagcca ggctaatatc tatgccattg gagacatggt ttcattgcat
     5101 tttggtatat tgggaatgga ttactataca cctttgatta accaagcaat gaagacaggt
     5161 caggctctgg ctctgcattt agctggatat cctattcctc cattacagac ggtcaaggta
     5221 ttaggtagtt ctcactttgg ttattatcgc gcgagtgttg gtgtgacaga agaagaagca
     5281 gagctttata tggatacgtg tagctatttg taccaaaatg ggaattctaa aaacctcttt
     5341 tggctgaaat taatagctag aaaaacggat gggattttaa taggcgctca gctcctatcc
     5401 aaaaccaatg ctcttgtcat cgccaaccaa ttaggtcagg ctctggctct gaaagttaca
     5461 gacggtgact tagcatttca agattttctc tttttacaag gtcacagtga tttggcttat
     5521 catcttcatg aagcttgtct caaattattt gaaaagaggc tccgccatga agattgaaga
     5581 cttaatggat aaagaacgaa aagctcaata tcgtctcttg gtgacccttt atcatgctaa
     5641 agaaacgtta aggctaaaag acctaatgag gctatcaaac ttgtctaaag tgaccctatt
     5701 aaaatacatt gacaacctaa atcatttgtg tcgagaacaa ggtttagcct gtcaattact
     5761 tttagaaaaa gacagtctga gcttaaaaga aaatggccag ttccattggg aggatttggt
     5821 agctctgctt ctcaaagaat cggttgccta tcagatattg acctacatgt actgccatga
     5881 acactttaac atcaccaact tatccgttga actgatggtt agtgaagcta ctctaaacag
     5941 acaactagct cacctcaacc agcttttatc agaatttgat ctggcgctct cgcaaggcag
     6001 acaactggga agtgagcttc agtggcgtta tttctatttt gaattgttcc gccatacctt
     6061 gaccagacaa ggcatagacg ccttggtcaa tcagttagac gcttcgcatt tggctacctt
     6121 aattgagcgg ctgattggcc agtcattatc ggcagaagcc ttagaacaat tattgatttg
     6181 gttggcgatt tcgcaagcgc gaatgtcctt tcaaaaaggc tacaatgatc attttttaag
     6241 agatagtgac tttatgacat ctaatatttt ctttaagcgc ttagaaagca tgcttttaca
     6301 ttacttaaga cgctatgccc ttgaatttga tgcttttgaa gctaagagcc tctttgtctt
     6361 tttgcatgct tatcctctac ttcctattgc cagcatgaag tatagtttgg gttttggagg
     6421 tcctattgct gatcacattt cagaagcctt atggctttta aagaaggctc atgtgattat
     6481 ccatcagaca aaggaggaaa tcatttatgg cttggggata tttttttcca aagcttactt
     6541 ttttaaagga gctatcctta gtcaaccaac caatagccaa tacctttatc aattagtagg
     6601 cgaagataaa agagcgcttt taagggttat cataaaccat ttagtgctgc aaatgggtca
     6661 ggaaacggat ttcagtcagc agttgagtga cgacatttta gtattgttga ttttttccat
     6721 tgagcgccac catgagcctc ttttagtagg gcttgccttg gggcaaaata aggtggaagc
     6781 agccattgcg gaattggcca ttagacgtca tttggggcat cgtagggatt ttcaattaat
     6841 gccctatgat catcagaaag tttatgattg tctgattacc tatcagactg tctgtttacc
     6901 gagacaagat ttaccctatt accggttaaa acagtatagc tccccctatg aactcactgc
     6961 cttagaagct tttctcaaag accttttcca gcaaaaaaat acacgagaag aggaactctc
     7021 actcagccca gcagctaagt ctagttttgc acacaaaact gtgtgatact tggtcttttc
     7081 taaagactgt tacactttta taataataaa gtaggatagt ctaaaggtag atgatttgaa
     7141 aaagcttatc aaatagagct taacaaaatc gaaaattttt ggaggtaaga aatgaaattt
     7201 ttcctagata cagctaatgt agccgctatt aaagcgatta atgaacttgg cgttgtagat
     7261 ggtgtcacca ccaatccaac cattatctca cgtgaaggac gtgattttga aacagttatc
     7321 aaagaaatct gtgacatcgt cgatggccca atcagtgctg aagtgacagg gttaacagca
     7381 gatgctatgg tagaagaagc tagaagcatt gctaagtggc atgacaatgt cgtcgttaaa
     7441 attccaatga ccacagaagg gttaaaagca acaaacattc tttcaaaaga agggattaaa
     7501 actaatgtga ccttgatttt cacagtaagc caaggtttaa tggcaatgaa agcaggcgca
     7561 acttacatta gcccatttat cggtcgtttg gaagatattg gcacagatgc ttatcaatta
     7621 atcagcgacc ttcgtgaaat cattgacttg tacgatttcc aagcagaaat catcgcagca
     7681 agtattcgta caacagccca tgtggaagct gttgctaaat taggcgcaca cattgcgacc
     7741 attccagatc ccttgtttgc taagatgacg caacatccct taacgacaaa tggtttgaaa
     7801 acctttatgg aagattgggc ttcctttaaa aaataaggct aaagagtctg tggccaacgt
     7861 gtcaacaggc tttcttattt gatagtcaaa aagaaagtga tatgagctta ttccataacg
     7921 tttttagtta tttttctagc agaaaagaat ttagaaactt tagaggattt tctgaaaaaa
     7981 acgtctattt ttgtgataca atagacaata gaaaacgttt aattttagtt gtaataaaca
     8041 gagaggtttt acttatgaca tttgatgcaa ttgaccagtt ggcagtaaac actgtccgca
     8101 ccctatcaat ggatgccatt caagcggcaa attctggaca cccaggactt ccaatggggg
     8161 cagcacctat ggcttatgtt ctttggaatc acttcatgaa catcaatccc aaaacaagcc
     8221 gtaattggtc aaacagagac cgttttatcc tatcagcagg tcatggaagt gccatgcttt
     8281 atagcttgtt gcatttagca ggttatgatt tatctgtaga agatttaaag aacttccgtc
     8341 aatggggttc taaaacacca ggtcacccag aagtgaacca cacagacggt gtcgaagcaa
     8401 ccacaggacc tcttggtcaa gggattgcaa atgccgttgg tatggccatg gcagaagctc
     8461 acctagcagc taaatttaac aaaccaggct ttgacatcgt tgatcactac acatttgctt
     8521 tgaatggtga cggtgacctt atggaagggg tcagccaaga agcagcaagt atggcaggac
     8581 atttaaaact tgggaaattg gtcttgctat atgattcaaa cgacatctct cttgatggtc
     8641 caacctctat ggctttcaca gaagatgtga aaggacgttt tgaagcttat ggttggcaac
     8701 atatccttgt gaaagacgga aacgatttag aagaaattgc agctgctatt gaagcagcta
     8761 aagctgaaac tgagaaacca accatcatcg aagttaaaac cattattggt tttggtgccg
     8821 aaaaacaagg aacttcagct gttcacggcg ctcctcttgg agcagaaggc attgcttttg
     8881 ccaaaaaagc ttaccaatgg actcatcaag attttgaagt gcctgctgaa gtaacagagc
     8941 gctttgctca aggtcttcaa gcgcgtggtg aaaaagcaga acaagcctgg aatgacttgt
     9001 ttgcagctta tgaagcagaa tatccagaat tagcagcaga ataccaaaaa gcctttacta
     9061 acgaagctgc tcaagtagaa cttgaagcgc acgaacttgg tagttcaatg gcgagtcgtg
     9121 tatccagtca acaagccatc caacaaattt cagaacaagt agcctctttc tggggtgggt
     9181 cagcagacct ttcagcttca aacaatacta tggttaaggc tgaaacagat ttccaaccag
     9241 gtcactacga aggccgtaac atctggtttg gcgttcgtga atttgccatg gctgcagcca
     9301 tgaacggtat tgcccttcac ggcgggacac gcgtttacgg cggaaccttc tttgtcttct
     9361 caaactatct tcttccagct gttcgtatgg cagcgctcca aaatttgcca acagtttatg
     9421 tcatgacaca tgattctatc gctgttggtg aagatgggcc aacgcacgaa ccaattgaac
     9481 aattggcaag tgttcgctca atgcctaact tgaacgttat tcgtccagca gacggtaacg
     9541 aaacaaatac tgcttggaaa cgtgccattg ctgaaacaga tcgcccaact atgcttgtat
     9601 tgacgcgtca aaaccttcca gtgcttgaag gaacaaaaga attggcagaa gatggtctta
     9661 acaaaggagc ttacatttta tcagaagcca aaggcgacct tgatggtatc atcattgcaa
     9721 caggttcaga agtgaaactg gcaatggata ctcaagaagc tcttgaagca gaaggcattc
     9781 atgtgcgtgt ggtatctatg ccatcacaaa atatctttga cgaacagtca gcagagtata
     9841 aagaaagcat cctaccagca gctgtgacta aacgtctcgc tatcgaagca ggttcaagct
     9901 ttggctgggc aaaatatgtc ggcctagcag gaaaaacatt gaccattgac acttggggtg
     9961 cttcagctcc aggaaatcgt atcttcgaag aatatggttt cactgtggca aatgcaactg
    10021 agttatacaa atcactctag gttacacatc ccaaacagag taaatcattt gatttactct
    10081 gtttttttaa aataaaatac ccagttcttt cttttcctca aactgcctaa tacacttctt
    10141 ttgataagaa gtcattgaca aaacaattca taaaaattat aatttaattg ttattgtatt
    10201 agcgtaaaag tacaaaaata aaggagtata aatggaaaag aaacgacagc gcttatacga
    10261 tgtgattaga cacgcttatg attatccaga aaatcgtgaa aatgtggcct tgtcgcagct
    10321 acttttagca gcttctaata gattaattaa acacagtaac ccacttcttg ttgcctacca
    10381 actaaaccaa gatgtggata attacttact tgataatgac attttacttc caaaatcgct
    10441 ttgccgtttt aagcagtcat tggaaaagta tattttagag gaagcctcat cagtctaggg
    10501 agaaaaagag aagaaaagga aaaaggatac catgatagaa tttaaacacg tttcaaagtt
    10561 gtatggcgac aaagaagctc ttagcgattt aaacgtcacc atcaatgacg gtgaaatttt
    10621 tggcctcatc ggacataatg gtgcaggtaa aaccacgacc atcagcattt tgacttctat
    10681 tatcgaagcg agttatggcg aagtatttgt ggatggtcaa cttctgacag aaaatagaga
    10741 agctatcaaa aaacaaattg cttatgtgcc agattctcct gatattttct tgaatctaac
    10801 tccaaatgag tattggcagt ttttagctaa aatttatggg gtgtcagatg aggaccgaga
    10861 agaacggtta gcacagctga caacactttt tgaactaaag gaagaagtta atcaaaccat
    10921 tgatagtttc tcacatggga tgcgccaaaa agtgattgtg attggtgctc ttgtatcaaa
    10981 tcctaacatc tggattttgg atgaaccatt aacaggtttg gatcctcagg cttcttttga
    11041 ccttaaggaa atgatgaaag ctcatgcagc ttctggacat acggtcttat tttcgactca
    11101 cgtgctatca gtcgctgagc agttatgtga tcgtatcggg attctaaaaa aagggaaatt
    11161 gatttttgtt ggaacgattg atgagttaaa agaacaccat cctgacaaag atttggagag
    11221 catctacctt gaactcgcag ggcgtaaggc gcaagaagag gggtgatggg atgaattggt
    11281 ctactatttg ggaacttatc aagataaaca ttctttattc caatccacaa agtttagcta
    11341 acctcaaaaa gcgtcaggaa aaacacccga aagaaaactt caaagcctat aaaagcatga
    11401 tgaggcaaca ggccttaatg attgccatgt ttttggtgat ttaccttttt atgtttattg
    11461 gtgttgattt cagtcattat ccaggacttt tctcctttga cgttgctatg ttttttatca
    11521 tgtcaacctt gacagccttt agctctcttt acaccatttt ttatgaaagt aatgacctaa
    11581 aactttatat tcacttacca gtaacgtcag aagaactcta tattgcaaaa attgtctcgt
    11641 cgctagggat gggtgctgtc tttttgatgc ctcttatctc tcttctctta attgcctact
    11701 ggcaactatt gggaaatcct ttgtctattt tagtagctat cgtgctcttt ttagtgttac
    11761 tagtgagttc gatggtatta gctatttata tcaatgcttg ggtgggcaaa atcattgtaa
    11821 ggagtcgcaa gcggaaactc atttcaacta tcatgatgtt tgtctcaact tttggagctt
    11881 tcgtcttaat ttttgcgatt aatattagta acaacaagcg tacgatgacg gatggcgtat
    11941 ttactgatta cccaactatt ccgtatttca aaggattcta tgatgttgtt caggcaccgt
    12001 tttctactgt ggccctcctt aacttttggt tcccattgtt acttatccta gctatggtat
    12061 atggtattgt cacaaaagtg atgccaactt attatcgtga ggttttttat attagtaacg
    12121 agaacaaagt caagcaaact aaaaaaccag ttaatcgtcc tcatcagaat caatcactgg
    12181 cgcagttgtt gcgaaaacat cacctattaa cgttacaaaa tgcaacttta ctgacacaaa
    12241 cctatctcat gcccctgatg tatgtgatgc tttttatcgg tccaagtttg tcacgtggca
    12301 caggtttctt taagcatatt tccccagatt actttggggt ggccttatta tttggggtta
    12361 gtttgggtgt catgtgtgca acaccaacca gctttattgg agtaggtatt tcacttgaaa
    12421 aggataactt tacctttatt aaaagcttac caataacgtt aaaaaaattc ttgctggata
    12481 aattttgctt gcttgtaggt ctgcaactga ttgtgcctat ggtgatttat cttgtgtttg
    12541 gtctctttgt gttgcacctc catcccctgc taacgattgc tttttgtctt ggctacgccc
    12601 tatcactgat tgtgcaaggg gaattgatgt atcgtcgtga ctaccggctt ctggatataa
    12661 aatggcaaga catgacgcaa ctctttacca gaggagatgg gcaatggtta acaatgggac
    12721 ttatctttgg taatttaata gttgcaggtg tgctaggttt tggagctgtt atcattgcca
    12781 atattatcca acaacctctg ttgattagta ttctattgag ttgtctaata ctaatggttt
    12841 taggtcttgc gcaattatgg attcaaaaaa ccttctggaa aagcttagag aggctttaac
    12901 gaagagggat cattattcgg aaaaacagtc ctctggggct gttttttatg ctataatttg
    12961 ggtatgatga aacgacaatt tgaagatgta acacgtatcg taattaaaat tggcactagt
    13021 tcattagtgc taccaacagg taaaatcaat ttagaaaaaa tcgaccaact ggcttttgtg
    13081 atttcaagtt tgatgaataa gggcaaggaa gttattttag tatcatcggg agcgatggga
    13141 tttggactag atattttgaa aatggaaaaa cgtccgacca atcttgctaa acaacaggca
    13201 gtttccagtg ttggtcaggt agccatgatg agtctttatt cacaaatttt tgcacactac
    13261 caaaccaacg tgtcacaaat tttattgacg agagatgtgg tggttttccc agaaagtcta
    13321 gccaatgtta ccaatgcttt tgaaagtctg attagtttgg gcattgtgcc gattgttaat
    13381 gaaaacgatg cggttagtgt ggatgaaatg gatcatgcga ctaaatttgg agataatgac
    13441 cgtttatctg ctgttgttgc tggtattaca aaagctgatt tattaatcat gttgtctgat
    13501 attgatggcc tttttgataa aaaccctact atttatgagg atgcgcagct acgcagccat
    13561 gttgcagtca ttacgcaaga aatcattgcg tcagcaggtg gtgcgggcag taaatttggc
    13621 acaggtggga tgcttagcaa aattcagtct gctcagatgg tatttgaaaa caaaggacag
    13681 atggtcttga tgaatggagc taatcccaga gatattttaa gagtgcttga agggcaacca
    13741 ctaggcacct ggttcaagca gatagaagag gtaacacatg actgatatga gaaggttagg
    13801 gcaaagggca aaacaagcta gtcttttaat agcacctttg tcgacgcaaa ttaaaaatcg
    13861 ctttttaagc accttagcaa aggcattagt tgacgataca cagactctct tagccgctaa
    13921 ccaaaaagat ttagcgaatg ctaaagaaca tggtatttct gacatcatga tggatcgctt
    13981 gcgcttaact tcagaacgga ttaaagcgat agcacaggga gttcaacagg ttgctgactt
    14041 agctgatcca atcggacaag tcattaaggg ctatactaac ctcgatggcc ttaaaatcct
    14101 ccaaaagaga gttcctctag gggtgattgc gatgattttt gagagtcgcc cgaatgtttc
    14161 agtggatgct tttagtctgg cgttcaaaac caataatgcc atcatcctaa ggggaggaaa
    14221 agatgccctt cactcaaata aagctttggt gaagttgatc cgacaaagtc tggaaaaatc
    14281 agggatcacc ccagatgctg tccagctagt tgaggatccc tcacatgctg ttgctgaaga
    14341 attgatgcag gcaacggatt atgtagatgt tttgattcca cgtggtggtg ctagattaat
    14401 tcagaccgtt aaggaaaaag caaaagtgcc tgtcatcgaa acaggtgtgg ggaatgtgca
    14461 tatctatgtg gatgctcagg ctgacttaga tatggctact aagattgtta tcaacgctaa
    14521 aacaaggcgc ccaagtgttt gcaatgcagc tgagggattg gtaattcacg aagctgtagc
    14581 agcaaggttt attcctatgc ttgaaaaagc gatcaatcag gttcagccag tagaatggcg
    14641 ggcagatgac aaggctttgc cgttatttga gcaggcagtg cctgcaaaag cagaggattt
    14701 tgagaccgaa tttttagatt atatcatgtc tgttaaggtt gtttcctctt tggaagaagc
    14761 tatttcttgg ataaatcagc atactagtta ccattccgaa gctatcatca caagggatat
    14821 taaggcagca gagacctttc aagatttagt ggatgctgct gctgtttatg tcaatgcttc
    14881 aacacgattt accgatggtt ttgtttttgg gcttggtgct gaaattggga tttctactca
    14941 aaaaatgcac gcccgtgggc caatgggctt agaagccttg acaagcacta aattttatat
    15001 taatggggat ggacatatca gagaataagc tactagaagg tcccttaatg actaatgaaa
    15061 gagaaaaaga agaagcaaag gttgctttag aagaactttt acatcagatt caggtgattc
    15121 atttggaatt gaaaattgtc cgaaattggg gctattttgt ttttttggag acggtatgct
    15181 tgtcagctgg atcaaacgtc gtgagcaagg ttaaacaatt attaatagac ttacaagtta
    15241 ctcttcaaaa ctgccgaaga tagctggcag acgtcgatag gctattatta gctgacttac
    15301 caaatatgag ttgggataaa acctttggta tttggttaga caacctgctg acagattggt
    15361 gtgtgattaa ggaagctaaa aatcttttac aagcagttga aattctagag gcagcggcta
    15421 agtcagtgct tgctaagttg gctgatagcg aacaaaaaga acaagattag agaagaggaa
    15481 gattcccacg agaatgaaat ccttttctct tactttttca tttctaaacc ttttgaagta
    15541 tggtacaatt aaagttatga caaaagaatt tcatcacgtg accgtactcc ttcacgaaac
    15601 agtggacatg cttgacataa agcctgatgg gatttatgtt gatgcgacgc taggtggctc
    15661 aggccactca gcttatttgt tgtccaaact tggtgaagaa gggcacctct attgttttga
    15721 ccaagaccaa aaggctattg acaatgcaca agttaccctc aaatcttata ttgacaaagg
    15781 acaggtaact tttattaaag ataattttag acacctcaaa gcacgtttaa cagcgcttgg
    15841 agttgatgaa attgatggta tcttatacga ccttggtgtt tccagcccgc aattggatga
    15901 aagagaacga gggttttctt ataaacaaga tgctccattg gatatgcgca tggatcgtca
    15961 gtcgctctta acagcttacg aagtggtgaa tacctatcca ttcaatgatt tggttaagat
    16021 ttttttcaaa tatggtgaag ataaattctc caagcagatc gctcgaaaaa ttgaacaagc
    16081 aagagctatt aagcctattg agacaacaac agagttggca gaattgatta aggcagcaaa
    16141 gccagctaaa gagttgaaga aaaaaggcca tcctgctaaa cagatttttc aagctattcg
    16201 cattgaagtc aatgatgaat tgggagcggc tgatgaatct attcaggacg ctatggaatt
    16261 attagccctt gatggtcgta tctcagtcat taccttccat tctctggaag atcgcctaac
    16321 caagcagttg tttaaagaag ctagtacggt ggatgtgcca aaagggcttc ctctaattcc
    16381 tgaagatatg aaacctaagt ttgaacttgt ttcacgtaag ccgatcttac ctagtcattc
    16441 agagttaaca gctaataaaa gggcacactc agccaagcta cgtgttgcca aaaaaattcg
    16501 gaaataagcc tatgacaaat gaaaaaagaa ctcaggcagt tacaaatgcc ttgcaaaaac
    16561 gaataaaaac tttctctaga attgagaagg ctttttatac tgctatcatt gtaacagcga
    16621 ttacgatggc agtaagtatt atttacctgc aaagtcgtaa gttgcagcta caacaagaaa
    16681 tcactagttt aaatagtcat atttcggatc aaaaactaga gcttaataat gccaagcaag
    16741 aagtaaatga gttatctcgc cgtgatcgta ttatagatat tgctggtaaa gcaggcttaa
    16801 gcaaccgtaa taataatatc aagaaagtcg agtaatctat gaaaaaatgg caaaaatatg
    16861 ttttagatta tgttgtgcgc gataggagaa ctccagtcga aaatcgcgtt cgagttggac
    16921 aaaatatgat gctcttaact atctttattt tctttatttt cattattaat ttcatgatta
    16981 ttattggaac agatcaaaag tttggagtta gtttgtcaga aggggcgaag aaggtttatc
    17041 aagaaaccgt tacgatccaa gctaagcgtg ggaccattta tgatcgaaat ggtacagcta
    17101 ttgcagtgga ttctacgact tatagcatat acgcaatttt ggataaatca tttgtctcgg
    17161 cttcagatga aaagttatat gtacaacctt ctcagtatga aacagtagct gatattttaa
    17221 aaaagcatct gggaatgaaa aaaacagatg taattaaaca gcttaagcgt aaaggacttt
    17281 tccaagtctc gtttggaccg tcagggtctg gcatttcata tagtactatg tctactattc
    17341 aaaaggctat ggaagatgcc aaaatcaagg gaattgcttt cacaactagt cctggtcgta
    17401 tgtatccaaa tgggacattt gcttcagaat ttataggcct agcatctcta acagaagata
    17461 aaaagacagg tgttaagagt ttagttggaa aaacaggtct agaagcttct tttgataaaa
    17521 ttttatcagg tcaagatggt gttattactt atcaaaaaga tcgaaatggg accacactct
    17581 tgggtacagg taagactgtc aagaaagcta ttgatggcaa agatatttac acaacgctat
    17641 ctgagcctat ccagaccttc ttagaaaccc agatggatgt ttttcaagcc aaatcaaatg
    17701 gtcagttggc cagtgcaaca cttgttaatg ctaaaactgg tgaaattttg gcaacaacac
    17761 aacgccccac ttataatgct gatactctga aaggacttga aaatacgaac tacaaatggt
    17821 acagtgcact tcatcaagga aattttgagc caggctcaac catgaaagtg atgactctgg
    17881 cagcggctat tgatgataaa gttttcaacc caaacgaaac ctttagcaat gctaatggtt
    17941 taacaattgc agatgctact attcaagact ggtctattaa cgaaggcatt tccacaggac
    18001 agtacatgaa ttatgcacaa gggtttgcct tctcaagtaa cgttgggatg actaaacttg
    18061 aacaaaaaat gggtaatgca aaatggatga attatttgac gaagttccgc tttggttttc
    18121 ctactcgttt tggtttaaaa gatgaagacg caggtatatt tccttctgat aatatcgtga
    18181 ctcaagctat gagcgctttt ggtcaaggga tttctgtaac ccagattcaa atgcttagag
    18241 cctttactgc tatttctaat aatggtgaga tgttagagcc acaatttatc agtcaaattt
    18301 atgatcctaa cacagcaagt tttagaacgg caaataaaga aattgttgga aaacctgtat
    18361 caaaaaaagc cgctagtgaa acaagacagt acatgattgg tgtaggaaca gaccctgagt
    18421 ttggaacact ctattcaaaa acatttggac caattattaa agtgggtgat ttacctgttg
    18481 ctgttaaatc aggaacagca caaattggtt cagaagatgg aagtggttat caagatggtg
    18541 gattgactaa ctatgtttat tcggttgtgg caatggtgcc agctgataaa ccagactttt
    18601 tgatgtatgt tactatgact aaaccacaac attttggtcc ccttttttgg caagatgtgg
    18661 ttaacccagt attggaagaa gcatacttaa tgcaagatac actaactaag ccagtagtat
    18721 cagatgctaa tcgtcaaaca acttataaat taccaaactt tgtaggaaag aatcctggtg
    18781 agacatcaag cgagttgcgt cgaaatcttg tccagccagt tgtccttggt actggcagca
    18841 agatcaaaaa agtatcgcat cagcccggtc aaacgttaac agaaaaccaa caagttctca
    18901 tattatcaga ccgttttgtg gaggtaccag acatgtatgg ctggacaaaa tccaatgtta
    18961 aaacctttgc taaatggact ggaatagaca tcagctttaa aggaacagat tctggtcgtg
    19021 ttatgaaaca aagtgttgat gttggtaagt ccttgaaaaa aataaaaaaa atgaccatta
    19081 ctttaggaga ttaacatgtt tttaaccctt attgcagcaa tcatatcctt tatggtatct
    19141 gctttcacaa tgccttattt tatcaaattc tatcaattga aaaaaattgg tgggcaacaa
    19201 atgcatgaag atgtcaagca acatttagct aaagctggaa caccgactat ggggggaact
    19261 gtttttcttt tagtagctac ggcagtatcg ttattggtta gtctattttc gatcaaaaac
    19321 actcaaagtt tagctttgat ttcaggcatt ttatcaattg ttgtcattta cgggattatc
    19381 ggatttttgg atgatttttt aaaaattttt aagcaaatca atgaaggatt gacagccaaa
    19441 caaaagttag ccttgcagtt ggttggagga ttgatgttct attttttaca tgttagtcca
    19501 agtggcattt cctctattaa tgtgtttggt tatcagctgc cgctgggaat tttctatcta
    19561 ttctttgttt tgttttgggt ggttggtttt tcaaatgccg ttaatttgac agatggtatc
    19621 gatggcttag cttctatctc agtggtgatt agtctagtga cttacggcgt tattgcctac
    19681 gttcagagtc aatttgatgt tttgttactg attggagcaa tgattggagc cttgcttggc
    19741 ttcttctgct ttaatcacaa acctgctaaa gtatttatgg gagatgtagg tagtttagcc
    19801 cttggagcta tgttggctgc catttctatt gcgcttcgtc aagaatggac tctcctgatt
    19861 attgggatcg tttatgttct tgaaacaagt tctgtgatgt tgcaagtgtc ctatttcaag
    19921 tacaccaaga aaaaatatgg agaaggtcgt cgtattttta gaatgacgcc tttccaccac
    19981 catttggaat tgggaggctt gtctggtaaa ggcaagaagt ggtcagaatg gcaggtagat
    20041 gcttttttgt ggggagtagg aagtctagct agtttactag ttttagctat cttatacgtg
    20101 ttctaagggg gattatcaag aatagtcacc aatagcattt tgattaacat catcaataag
    20161 agagtggaga tgacccacaa atgctgattt tgtgagtgtt gtcccactcc ttttgcttgt
    20221 ctctaacaat aatgatataa tatggaagaa aacgaggtaa aacatgtcat ttaaagatta
    20281 tcattttaag caatacgttc agcaagcgct ggaagaaatt ggctttgtca atccaacaga
    20341 ggtccagaag cggctgattc ctattgttaa ctccggtcgt gacttagttg gggaatcaaa
    20401 aacaggttca gggaaaaccc acaccttttt gttgcccatt tttgaaaaac ttgatgaagc
    20461 taaagcagaa gtgcaagtcg ttattacagc acctagccgt gaattagcaa ctcaaatttt
    20521 tgatgcttgt aaacagattg ctaagcactc ccaagaagag attcgtttgg ccaactatgt
    20581 tggaggcaca gacaaactcc gtcagattga aaaactaaaa ggcggccagc cgcatattgt
    20641 catcgggacc ccaggtcgta tttatgactt ggtcaagtct ggtgatttag cgattcataa
    20701 agcaacgacc tttgttgtgg atgaggccga tatgaccatg gacatgggct ttttggatac
    20761 ggtagataag attgcagcct ctcttccaaa atctgttcag attttggtgt tctcagcaac
    20821 tattccacag aaattacaac cctttttgaa aaaatacttg accaatcctg ttattgaaca
    20881 aatcaagaca aagaccgtta tcgcagatac cattgataac tggcttgtgt cgacaaaagg
    20941 gcgtgataag aatggtcaac tgttagagat tctcaaaacc atgcagcctt atatggctat
    21001 gctttttgtc aataccaagg aacgcgcaga tgatttgcat gcctttttaa cagctaatgg
    21061 cttaaaagtg gctaagattc atgggggcat tcctccaaga gaacgtaagc gcatcatgaa
    21121 ccaagtcaaa aaactggact ttgaatacat tgttgccaca gacttagcag cgcgtgggat
    21181 tgacattgaa ggggtcagcc atgtgatcaa tgacgccatc ccacaagact tgtctttctt
    21241 tgtccatcgt gtaggccgaa caggtcgaaa tggtatggca ggaactgcca ttacacttta
    21301 ccagccaagt gacgactcag atatcaaaga gctggaaaaa atggggattg cctttacacc
    21361 gaaggtgctc aaaaatggag aatttcaaga tacctatgac cgtgatcgtc gtcaaaaccg
    21421 tgaaaaagcc tatcaaaaat tagacacaga aatgattggt ttggtcaaaa agaaaaagaa
    21481 aaaagtcaaa ccaggttaca agaaaaaaat tcagtgggcg gttgatgaaa aacgtcgcaa
    21541 agaacgtcgt gctgaaaatc gtgctaaggg ccgcgctgag cgtaaggcca aaaaacaaca
    21601 tttttaagaa caaagagcaa gcaagtgatc aattggcatc taatcttgct ttttttacac
    21661 ctgctacttg tattgtttac catttgttaa cacaaaaagt gatcatagct tttgcttatt
    21721 gctataatac ggatttacta ttttacatgt ggatctaaga gtgttagaat attaagacaa
    21781 acaaatacaa aggggaaata catgacatca gtgtttttaa ccagtggttg ggctttttat
    21841 gatcacctca tctcaccgat tccacatggg aaacttttta gttggcatgc tgtttttgat
    21901 gccattccaa atattatcca acggcttcca attacgcttg gtttgacact atcaggagca
    21961 acctttggct tggttttggc tttgattttt gcccttgtca aaattaataa agtgaagcta
    22021 ctatacccta ttcaagctat ttttgtgagt ttcttgcgag gaactcctat tttggtacag
    22081 ttgatgttga cctactacgg tattcccctc tttctaaaat gtctcaatca gaagtatggc
    22141 tttgattgga atgttaatgc aattccggct tctatttttg ccattacagc ctttgctttt
    22201 aatgaagcag cctacgctag tgaaactatt cgggcagcca ttttatcagt agatacaggt
    22261 gaaattgaag cagcaaaaag tctaggcatg acttctgtgc aggtttaccg tcgtgtcatt
    22321 attcctaatg caactgttgt ggctattcca accttgatta atgggttaat tggcttaact
    22381 aaaggaacat cgcttgcctt taatgcaggg attgtcgaaa tgtttgccca ggctcaaatt
    22441 ctaggcggat cagattaccg ttattttgaa cgttatatct ctgttgctct tgtctattgg
    22501 tctatcagta ttttgatgga gcaagtaggt cgcttgattg aaaacaagat ggccatcaaa
    22561 gcaccagaac aagctagaaa tgaaaagtta ggagaattgc gttgatgatt acaattagaa
    22621 atttgagcaa gacattctca gagcaaaaag ttttagatag tttggctctt gatattgaaa
    22681 aaggtcaagt gattgcttta gttggtgctt caggagcagg gaaatcgact ttccttcgta
    22741 gcctaaacta tttggaaaaa ccagattcag gctccattag cattggtgac tttacggttg
    22801 attttgagac cattaccaca gagcaagtcc ttattttacg tcggaaactt gccatggttt
    22861 ttcaagaatt taatcttttt gagcgtcgta cagcccttga gaatgtcaaa gaaggtctta
    22921 aggtggtcaa aaaactgtct gaccaagaag cgactaaact ggcgcaagca gaattggcta
    22981 aggttggttt agcagatcga aaacatcatt acccacgtca tttatcaggg ggacaaaaac
    23041 aacgggtagc tttagcaaga gctctggcta tgaaaccaga tgttttatta ctagatgagc
    23101 cgacctcggc tcttgacccg gaattagttg gcgaagtgga aaagtctatt actgatgctg
    23161 ctaaatccgg acagaccatg gttttagtga gtcacgacat gaattttgtt taccaagtgg
    23221 cagatagagt actcttttta gatcaaggga aaatcttgga acaaggaaca cctgaagaag
    23281 tctttagaca tcctcaaaaa gaacgaacca aagaattttt tgcaagctat tcaaaaacat
    23341 acatttaatg ttattctttt gttataataa aaaggtctag gtattcctgg gcttttcttg
    23401 ttatgttttt tagaaaaact tatgggaaag aaataatgac taggtggaaa tatgttagta
    23461 gaaatcttta gcctttatgt aaaaggtctc attctctcaa gccttactat gatgttaatt
    23521 tgtttaatat ggactttatg gcgagcaaaa accaaaagag ataaaacatt gattgaaaga
    23581 caagcttttc tttatgagat gctaatggtt gctattttaa ccatcccaat tttatcgttt
    23641 gcttttatga gtattttggt tgtcataaaa tcctaatcat tgcaagaagg gattatcatt
    23701 gctaaaatat tggcaagagt agattaaagg agaaaaatca tgtacgatac attaattatt
    23761 ggttcaggac cagcaggaat gactgccgct ttatacgctg ctagaagtaa tttatctgtt
    23821 gccattattg aacaaggggc acctggtggt cagatgaaca acacttttga tatagagaat
    23881 tacccgggat atgatcatat atctggacca gaattagcta tgaaaatgta cgaaccttta
    23941 gagaagttta acgttgaaaa catttatggt attgttcaaa aaatagaaaa ttttggtgac
    24001 tataagtgtg tgtcgactga agatgctagt tatgaagcta aaacggttat tattgcaacg
    24061 ggagctaaat accgtgtact aggtgttcca ggtgaagaat actatactag tcgaggagtt
    24121 tcttattgtg cagtatgtga tggtgcgttt ttccgtgacc aggatctttt ggtcgttggt
    24181 ggtggtgact cagcagttga agaagctatt tatcttactc agtttgctaa aaaggtaacc
    24241 gttgttcatc gccgtgatca actaagagct cagaaaatct tgcaggaccg tgccttcgct
    24301 aatgacaaag ttgactttat ctgggattca gttgtcaaag aaatcaaagg taacgacatt
    24361 aaggtctcca atgtcctaat cgaaaatgtg aaaacaggcc aagtcactga ccacgctttt
    24421 ggaggtgtgt ttatctatgt tggtatgatt cctgtgacag gtatggtaac ggatctagaa
    24481 attaccgatc aagaaggttg gattatcaca gatgatcata tgaggacaag tattcctggt
    24541 atttttgcga ttggagatgt tcgtcaaaaa gaccttcgcc aaatcacgac agctgttgga
    24601 gatggtgcaa ttgcagggca aggtgtttat cactaccttg aaagtttccc ttcataaggt
    24661 taaaactcct tcatttttcc ttgtctttcc cttaaaaaag ctaa
//
LOCUS       DAUCLR010000024        22830 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.024, whole genome shotgun sequence.
ACCESSION   DAUCLR010000024 DAUCLR010000000
VERSION     DAUCLR010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22830
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.024"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            679..1065
                     /locus_tag="VGT87_000749"
     CDS             679..1065
                     /locus_tag="VGT87_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEQ3813626.1"
                     /translation="MDEKKLFENFQLTFGRMISPFEIEDIQKWIHEDNMPIEVVNLAL
                     REAVENNKISWKYINKILVDWYKSGDTTVEKVRDRLQRFDDSKKQRSVTTSNVPSWSN
                     PDYKEPDLKEFALGSMDGIEDGSGDF"
     gene            1046..1279
                     /locus_tag="VGT87_000750"
     CDS             1046..1279
                     /locus_tag="VGT87_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813627.1"
                     /translation="MDQEIFNFFNKQIKKDFGKTASKETFAKFASYCAEGIEKNGVKP
                     IFNWINLYAFGTGMTTAEADRLRIERYKQENAL"
     gene            1276..1416
                     /locus_tag="VGT87_000751"
     CDS             1276..1416
                     /locus_tag="VGT87_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813628.1"
                     /translation="MTKQHRETLIWYRASHQERERLLDFGLVDKARYVTLLRQLRKKY
                     AI"
     gene            1425..1631
                     /locus_tag="VGT87_000752"
     CDS             1425..1631
                     /locus_tag="VGT87_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813629.1"
                     /translation="MTPEQAEKAKIRAKQELETFSIYLDQAIDDLGGVLTSREVFLAA
                     GITYLGAGQTDIHAAVEGLCEQIQ"
     gene            1687..2017
                     /locus_tag="VGT87_000753"
                     /pseudo
     CDS             1687..2017
                     /locus_tag="VGT87_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017991.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2020..2946
                     /locus_tag="VGT87_000754"
     CDS             2020..2946
                     /locus_tag="VGT87_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HEQ3813630.1"
                     /translation="MANQLSTYTHKQFFNAPTVQKAFDDVWKGAGTQFAVSILSVLQG
                     SQSLKSASNKSIYAAAMKAAVLNLPIEPSLGRAYLVPYKGQAQFQLGYKGLIELAQRS
                     GQYKNINAGIVYKSQLISYNPLFEELILDFSKPQDEIVGYFAAFKLFNGFEKVSFWTV
                     EKVTAHGKKFSKSFASGPWKTDFDAMAQKTILKDILSKYGPLSVEMQKAIEEDNQDST
                     ISTPKDITPQEANSLDDLIGHQNENKDAPNNLKDVTEDLHDEEEKTLTDENKTVLEDT
                     SYPADEVPDFDQETGEVLASEGNLFDNLGDLI"
     gene            2943..3143
                     /locus_tag="VGT87_000755"
     CDS             2943..3143
                     /locus_tag="VGT87_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813631.1"
                     /translation="MIKLVKIEGYYINPEYVVGVWERSALNFDDLDEKVVVIQFVGDR
                     EREEFVFCDTPIDEVIKRLLDD"
     gene            3136..3933
                     /locus_tag="VGT87_000756"
     CDS             3136..3933
                     /locus_tag="VGT87_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HEQ3813632.1"
                     /translation="MTSLDLLGKDYYSRESAIRYWSISQYKRFKECEARALAELQGNW
                     TDTRDNTALLVGNYVHSYFESKKAHEEFKAQNGSEMISTRGATKGQLKKDYLVAEQMI
                     DALKSDSNFMAIYQGEKEAAITGFLGEIEFKGKIDCLNVERGYFVDIKTTKGPIDDTI
                     WSGEERVRWFEAYGYILQMAAYKTMLEAKYNKPFEPIIYAVTKETPPDTRAIRIQNVD
                     AMQNELDSLAQSIKRLDDVKKGIEKPKPCGHCEYCRANQLTQRVMIF"
     gene            4298..4693
                     /locus_tag="VGT87_000757"
     CDS             4298..4693
                     /locus_tag="VGT87_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813633.1"
                     /translation="MDELKEKALSKMLDEMNKKHSAAEDAIHNWLSDQDDEELFKGIL
                     DDKKSIKDAMKYCINQAQKQKTGNCAMVDDKTVFGWVRVYFTGKTKKIEPVQATVTVS
                     QENTKVIPKKNKKSKGVVEGQLDLFGALV"
     gene            4690..6036
                     /locus_tag="VGT87_000758"
     CDS             4690..6036
                     /locus_tag="VGT87_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfJ domain-containing protein"
                     /protein_id="HEQ3813634.1"
                     /translation="MKKNQKIIEGRLKPPKAFFDWCYEQIPTIRWQNKEKTIETEREH
                     KVYIDNRRLTKRTRLDFFDKFYSFAIVLVTSKRIEIQSYGFYSRYSNGRQSIEVKLCN
                     FEKLANNHHIKLTEQFGQYVPGLTPNFIGSGAYSGTKFYPDDWEEKIKSISELKYLQF
                     PIGLAHHNLPHMYKYRTQIEFLQKIDAWKIANDLAYDVTEYDAWHVRKAVDCRVITKK
                     WLHQNKQFFKNSNRSFREYELERRIKARNGKLVPGIEKILSYQDINKIPKAAKINRFQ
                     NWLVKNKVDFNYYIDYLSMLDELGIEIDTDNLIMPKDLVKAHDNVVKLVNQKKSEIEK
                     RKFKNRLKSLSKYEQTIGDYCFRAPVDSGELIREGKTLSHCVGSARYTQAHASGKTTI
                     IFIRRKSDSDKPFYTMEYKAGHIVQVRGKHNQSATEEVQKVVDQWLAIVNKNYKHA"
     gene            6047..6379
                     /locus_tag="VGT87_000759"
     CDS             6047..6379
                     /locus_tag="VGT87_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813635.1"
                     /translation="MITKINVPKTSIVIEIENKEITIENLINYDIKMIFRNQDNEPSL
                     DENGDIFEPMYWLDIKAKPNDEIEYHSSLGVKKEKRRLAELQIFFEYIEANKQNLFDL
                     CGLKGELA"
     gene            6376..6888
                     /locus_tag="VGT87_000760"
     CDS             6376..6888
                     /locus_tag="VGT87_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813636.1"
                     /translation="MSSLTLSLDVSTTGTGWAIFDGSTLIQSGASKPKQKSFYERAKS
                     MASELKTIQLRAIQKHDKPFEDIVIEQNTVLGPNQQSSIKIGIATGIILGRLLAEEIY
                     FVNVSTWRKYWKFSYKDRSKKSMKKQAINAVAAEFNKQVKDDEADAILIGSYFTNLGK
                     DFGDLESHKN"
     gene            6924..7241
                     /locus_tag="VGT87_000761"
     CDS             6924..7241
                     /locus_tag="VGT87_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1372 family protein"
                     /protein_id="HEQ3813637.1"
                     /translation="MEIVKDGVKFIASMIIVLAATFGCGYLFYRAGYEQAKKEVQPVT
                     IYTVDNVGGVMVGEITDKEIIEGRYTVTAHAYGKFLVTKEQYKAIKVGDKIPDYLKKR
                     GTK"
     gene            7238..7393
                     /locus_tag="VGT87_000762"
     CDS             7238..7393
                     /locus_tag="VGT87_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813638.1"
                     /translation="MIELTQKQADYVEFVRKTIVDPDMMEFAVNTQLISYALLVGYCI
                     VDEEVAK"
     gene            7390..7641
                     /locus_tag="VGT87_000763"
     CDS             7390..7641
                     /locus_tag="VGT87_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813639.1"
                     /translation="MKPRKYPYSGKIRIIKKELPRFVRLGDFAFNSNLVKHIDKIRQV
                     KPNETLIRFKIPKLFMTYEEETFKVRLEIDKVVKILNQY"
     gene            7717..8136
                     /locus_tag="VGT87_000764"
     CDS             7717..8136
                     /locus_tag="VGT87_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEQ3813640.1"
                     /translation="MSKAKAILKDLRNLDLYIASLIRRQEKIETSLLSSPKWSSDKVN
                     GGIKRKQDDVYVELIATAEDIEKKTAEAIRKQRELQNLIDSLENTDSQTILSMVYIDK
                     MTRWQVIDELNCSESTYFRLLRVATKELNNLTVNDSD"
     gene            8244..8588
                     /locus_tag="VGT87_000765"
     CDS             8244..8588
                     /locus_tag="VGT87_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEQ3813641.1"
                     /translation="MSQLRADKKGTHRVAFEKNKRKLLKTTNLCGICGKPVDKTLKYP
                     HPLSAAIDHIVPIAKGGHPSALENLQLTHWQCNRQKSDKLFANQASNEPKTIGNRNLP
                     QSRDWSSFTLKK"
     gene            8736..9092
                     /locus_tag="VGT87_000766"
     CDS             8736..9092
                     /locus_tag="VGT87_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813642.1"
                     /translation="MKKKCLICKKNFQAKTNRTLYCSEECRKKGNREKQRKLMKQKRA
                     EQRKEKKKVLNPNTDVTEKPKKIRNLAQHYKKLKKEILANESEFGFTGITLIEGIDVH
                     EENFVDLVMQKIKEQK"
     gene            9089..10357
                     /locus_tag="VGT87_000767"
     CDS             9089..10357
                     /locus_tag="VGT87_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEQ3813643.1"
                     /translation="MNYMGMGYLRRKLALFKTGVDKRYRYYAMDDRDDTRSIVMPNNV
                     REMYRSVLEWTAKGVDSLADRIIFREFTNDDFNAWEIFKANNPDIFFDTAIQSALIAS
                     CCFVYIMPGAEDGLPKMQVIEASKATGILDPTTFLLTEGYAILESDSNGNPTLEAYFT
                     DKDIWYYPKKGKPYNIKNPTGHPLLVPIIHRPDAVRPFGRSRITKAGMYHQKAAKRTL
                     ERAEVTAEFYSFPQKYVLGMDPDAEPMEKWRATVSTLLEISKDEDGDKPTVGQFTTAS
                     MAPFMEHLKMYASLFAGGSGLTLDDLGFPSDNPSSVESIKAAHENLRAAGRKAQRSFS
                     SGFLNVAYIAVCLRDEFPYLRNQFMDTVIKWEPLFEADANMLTLVGDGAIKLNQAIPG
                     FMDADVIRDLTGVKGADKPIPAITEVTTDG"
     gene            10350..11843
                     /locus_tag="VGT87_000768"
     CDS             10350..11843
                     /locus_tag="VGT87_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813644.1"
                     /translation="MVDDVLPKLLKSVRQDFEKYFGESDVVTKAFAELQAKKVTYKTV
                     NEFAIEVGRLLSLALTGSVSSDKLPDGKMYYNIAKRLLDETMGRNYKLISGYAGDVQR
                     ILNENAQIGLKVQRPPLNRDKINGMVNRLDSENTFDDVKWLFGEPIVNFSQSIVDDTI
                     KANADLQYKTGMTPQVVRTESGNCCEWCREVVGTYSYPKVPKDVWRRHQRCRCTLDYD
                     PKNGKVQSAWSKIWRKKEKTQESIERVEKFKESALVESIKNDIAKLDMTKVGPSDIID
                     IGKRINYHFRVSEHIGDKEKLKEIFSNFREIGGEIPKNTWAKGSSKLVKDQLQEAFQN
                     YPTEWAAVPDGIGKKLKAIKRKRGYFDGYDEDLVIATNGTRKTTPYHEIGHMIELVNP
                     DLVRLEKAWVDKRTANEAEVRLKDIFPSSNYGIGEVTKKDDFISPYIGKYYSDAAEVF
                     TMGLQGIFVPEERFAKSFDKKTWKYDYKTINDDPEFLNFIIGLFVKV"
     gene            11849..12073
                     /locus_tag="VGT87_000769"
     CDS             11849..12073
                     /locus_tag="VGT87_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813645.1"
                     /translation="MEPKLHRQLRQKYDDAEKQYLEKFGEDSLDRVFFWEPDVYFDEW
                     KKVLPDATLELNKAINSGVAIDPDPENAIY"
     gene            12150..12302
                     /locus_tag="VGT87_000770"
     CDS             12150..12302
                     /locus_tag="VGT87_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813646.1"
                     /translation="MTWENKDEFQQIMNKVNETKEAYEKALEAAENFVPKMSCATKLN
                     KGEGNE"
     gene            12295..12561
                     /locus_tag="VGT87_000771"
     CDS             12295..12561
                     /locus_tag="VGT87_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813647.1"
                     /translation="MNKRIKKKRKLETAIMLLIAENALQAEAIKNQNKEIMELKSIVQ
                     RNALATNEELATVKAATLDNQSVIKAIGDTVDYIKKNYKRKWGK"
     gene            12563..12778
                     /locus_tag="VGT87_000772"
     CDS             12563..12778
                     /locus_tag="VGT87_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813648.1"
                     /translation="MKYRKKPVVVEAIQFTGTNYEKIKEFIGQNTLCSTLSIVIPTLE
                     GDYIIKGIAGEFYPCKPDVFEQTYEST"
     gene            12860..14275
                     /locus_tag="VGT87_000773"
     CDS             12860..14275
                     /locus_tag="VGT87_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEQ3813649.1"
                     /translation="MVTKTKTKLGNQRPTQSVNLHFAKSLAHEAINYYKKTGLSCYPW
                     QVNMLIPIMAIDENGLWVHQKYGYAIPRRNGKTEVVYIVELWALHKGLKILHTAHRIS
                     TSHASFEKVKKYLEMSGYVDGEDFISNKAKGQERIEFKASGAVIQFRTRTSNGGLGEG
                     FDLLIIDEAQEYTSEQESALKYTVTDSDNPMTIMCGTPPTMVSTGTVFEAYRKDCLKG
                     NKRYSGWAEWSVPEMVKINDVSSWYISNPSMGFHLNERKIEAELGEDEIDHNIQRLGY
                     WPSFNQKSVISEKEWAKLKVEQVPELKSKLFVGIKFGQDGNNVSLSIAARTSENKVFV
                     ETIDCLSVRNGTQWIINFLKSADIAKVVIDGASGQELLAQEMKEQGLKKPELPKVAEI
                     ITANMMWEQGIMQETICHSDQPSLTAVVTNCEKRQIGSNGGFGYKSLYDDRDISLMDS
                     ALLAHWICYTTKPKRKQRTSC"
     gene            14356..14817
                     /locus_tag="VGT87_000774"
     CDS             14356..14817
                     /locus_tag="VGT87_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEQ3813650.1"
                     /translation="MSEFKVIETQEELDTIVKARIAREREKYQDYDQLKTRVEELETE
                     NSSLQTALNDAKSNTDSYTEEISTLKNQIADYETANLRTKVALQYGLPIDLADRLQGD
                     DEDGLKVDAERLASFIKPSQPQPPAKSNEPNIDSNADANYRALVQGLSTED"
     gene            14842..15753
                     /locus_tag="VGT87_000775"
     CDS             14842..15753
                     /locus_tag="VGT87_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEQ3813651.1"
                     /translation="MGTETSKASLFDKHLVSDLINKVKGHSSLAKLSSQKPIPFNGSK
                     EFTFTLDSDIDVVAENGKKTHGGLSLEPVTIVPIKVEYGARLSDEFLYATEEEKIDIL
                     KAFNEGFAKKLARGIDLMAMHGINPRTKKASDVIGTNHFDSKVTQVVKFTESEDADAN
                     IEAAVNLIQGAEGVVTGLAMDTEFSTALAKVTNGEMGPKMYPELAWGANPDSINGLKS
                     SVNTTVGAGADEAESKDLVIIGDFESMFKWGYAKQIPMEIIKYGDPDNSGKDLKGYNQ
                     IYLRAEAYIGWGILDAKSFARVTKGEV"
     gene            15753..15953
                     /locus_tag="VGT87_000776"
     CDS             15753..15953
                     /locus_tag="VGT87_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813652.1"
                     /translation="MLYVNSKTGASFYSSVPVCGGDWTLVEQQTGEKAKTVAEIKRQL
                     DAAGIKYSSKAKKPELEALLPG"
     gene            15963..16385
                     /locus_tag="VGT87_000777"
     CDS             15963..16385
                     /locus_tag="VGT87_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage Gp19/Gp15/Gp42 family protein"
                     /protein_id="HEQ3813653.1"
                     /translation="MGNFATTDDVILLWRPLSVDELKRANALLKVVSDTLRMEADKVG
                     KDLDKTMVDKPYFVNVIKSVTVDIVARTLMTSTQGEPMSQESQSALGYTWSGTYLVPG
                     GGLFIKDNELKRLGLKKQRYGGIELYGEIKRDNDYFDR"
     gene            16345..16683
                     /locus_tag="VGT87_000778"
     CDS             16345..16683
                     /locus_tag="VGT87_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813654.1"
                     /translation="MGKLRGITITLIDKVTIDIDPFGNPIKKDKEISVDNVLVSPATS
                     DDITSQLSLSGKKAVYTLAIPKGDNHDWGDKEVRFFGEKWRTVGLALEGIEELIPLEW
                     NKKVMVERYE"
     gene            16676..16912
                     /locus_tag="VGT87_000779"
     CDS             16676..16912
                     /locus_tag="VGT87_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813655.1"
                     /translation="MSKFKFKLNKAGVAELMKSSEMQQVLTTKATAIRERCGDGYAQD
                     IHVGKNRANAMVSAKTIKAKKDNSKNNTLLKAVR"
     gene            16913..17248
                     /locus_tag="VGT87_000780"
     CDS             16913..17248
                     /locus_tag="VGT87_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813656.1"
                     /translation="MIEVIIKKYLDEHLDVPSFFEHQKDEPARFIILEKTSGAKQNHL
                     LSSTFAFQSYAESLYEAALLNDKVKQVIEQLDVLPQVSGVHLNADYNFTDTATKRYRY
                     QAVFDINHY"
     gene            17264..17854
                     /locus_tag="VGT87_000781"
     CDS             17264..17854
                     /locus_tag="VGT87_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEQ3813657.1"
                     /translation="MANDTKNVTSAKPKAGGAIYSAPLGTTLPEDAKSKLDTKFKNLG
                     YVSEDGVVNEDTRSSENIKAWGGDIVGSVQTEKEDKFTYKLIESLNVEVLKEVYGAAN
                     VTGDLDKGIHIKSNSKELEAHAIVIDMIMNGGILKRIVLPNAKVDEVGEIKYVDGEVV
                     GYETTLKCFPDEKGDTHHEYIVKPGEANKKENSFEM"
     gene            17865..18128
                     /locus_tag="VGT87_000782"
     CDS             17865..18128
                     /locus_tag="VGT87_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813658.1"
                     /translation="MEILKGKTTSGFEYEIPKKRLKNFELVEAIAEEETDPTAVVKIV
                     NLLLGDAAKSLKEHVRDAEGIVDVEAIGVEIKEIFESQKDLKN"
     gene            18143..18514
                     /locus_tag="VGT87_000783"
     CDS             18143..18514
                     /locus_tag="VGT87_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5361 domain-containing protein"
                     /protein_id="HEQ3813659.1"
                     /translation="MIAKDDDALTCDLAETYGIYDYRQLPAYQVAVFAVGLRSNSRIK
                     MALSGETEALDTVLLAGIYDNTNLLFWSKTKDGQSGQNKPKSVVEAISGSKSQKANDV
                     ISFASGEDFENARKQLLGGDG"
     gene            18514..20877
                     /locus_tag="VGT87_000784"
     CDS             18514..20877
                     /locus_tag="VGT87_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813660.1"
                     /translation="MATELGQAYVQIMPSARGISGAISKQLDPEARSAGLSAGKLFGS
                     KLKLAALAATAGLGILLKKTISASLSTGADLQQSFGGIDTLYKGAETAVKGFAKEAYK
                     AGISANTYAEQAVSMGASLKQSLGGDAVAAAKAANMAIMDMADNSAKMGTDITSIQMA
                     YQGFAKQNYTMLDNLRLGYGGTKEEMKRLLSDAEKLPAAMGKKFDLSNYADVVEAIHL
                     VQDNMGIAGVAAEEAKTTFSGSLAAMKSSFTNVLAGLSLGDDIRPALRGLAETTSNFL
                     FGNFIPMVANIFKGLPSAIGTFIGAATPIIASQFKGLMSSLGISIDLSPITAKFAQIG
                     QNLQPVFNGLKTAFSQLPSFFTSIGSAVAPVIDTIISGLARLDFSGFEALISAILPAI
                     QTGFQTFMSIVGPAISQLVNSFVNLWNACQPLVTILSGALMPAFQILGAFLGGVLQGI
                     LGSISFAFDALKVVIEFLTPIIDWLVQGFNAISPALQIVAQWVGNVIGIFTSLGASGQ
                     GLSSMLSSAWGNIQTVVSTAKNMITLAIDGIKLVFSNLGNAGNILKGLLSAAWSAMQN
                     AVVIAKGIINSAISAIKTAFSSFGNLVSSVSGTIKSVIGSLKNAFYSLASIDLVGAGR
                     AIMQGFLNGLKSMWGAITGFVGGIADWIRKHKGPISYDRVLLKPAGKVIMQGFNNSLM
                     DGFKEVKSNVSGMADDLAGTITGKSLSLGIDAKPSVTADDLLSSNISTKTTVGSATSD
                     LSLFFVKVLALLQDILDKNTDVYLDKEKVSAILYEEFAKIMAREGIA"
     gene            20874..21569
                     /locus_tag="VGT87_000785"
     CDS             20874..21569
                     /locus_tag="VGT87_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813661.1"
                     /translation="MIPKVIIDGFDTSTIPNCVLTGYDVGDVLSPHFVENEVYGMNGT
                     SRGLESYNESKPTLTWHLSTFDDAVNLVNHLDGLGKIIEFWHIPKSFYYYDCLSVKIN
                     AVTMNSWRVTLKLALYPFRYAKGISDVTIAGNGSINNPGNVFSEPKIIVEGTGKGTLT
                     IDKQVMELNLSGKATIECKHGQQCVYDGEGNVKNSIRIRGRFFEIQPGTQGIAVSGGI
                     ARLTISPRWRYKV"
     gene            21551..>22830
                     /locus_tag="VGT87_000786"
     CDS             21551..>22830
                     /locus_tag="VGT87_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEQ3813662.1"
                     /translation="MEVQGLISIKDDNTPLVAAFEDEITQEANSDYKLSFKYPAKHEY
                     RPLIKKGIILEADDLHGTQLFRVFEITKRHGYINVYGNQVADDLNGYAIDSISVDRVQ
                     GMTVMSELAGNIKREHPFSFFSDIDGRHTFNQSDVSVMDALANGKHSIMGQWGGELVR
                     DKYQVNLLKKAGKDTETLFMYKKNLKSYEETDTIKGLISILHLVAEVEEQREEESREV
                     SDADIGHNEVSKKKTIRVTVESKLKDTHPIIVEKTIKVQDQDVKTEEDLLAYGKKYFE
                     KTLCDIPGNSLKIDVTNNYEGAVRLFDTAIVFHELYDRDLRMQITGYRFAPMANRLKS
                     IIFGEIKTNLAKQISNQIDNKVAESTAQHDAAFEAKLQKQIDNANRIFDTKEAKLREE
                     IEDGIKKAEANAEVKVAEVNAKVLEAEELAKAVD"
BASE COUNT         7992 a         3695 c         5009 g         6134 t
ORIGIN      
        1 agaattattt ttgttagcaa caaccgttat cttagcggtc attgctaggg tgcaacacga
       61 agtcattaaa aaacacaatt cgccagagaa taagcggaaa atttttaggg aagtagcttt
      121 aaaaaacagc aaaggatgga gcaagaagcg tgcaagagga ggggttgtca gctaatgcag
      181 tacatttttc aagacattaa caggtagtta cacagctgtt agcaatgaat ttattcagga
      241 caaagaactt ttcaataagg aaaaaggact gctgctaacc attttgagta atgcagacga
      301 atggagagtc tatcctgaag aactcgctag acgttgtaag gatagcgaat ctgcaatcag
      361 aagccagttg aaagcgttag agaatgccaa gtatatcaga acttatagaa aatcatttgg
      421 cgggcgatat ggtaccgaag cctatagatt ctgttctgac agaaaaataa gcgacgaggc
      481 gttcaatact ttgaaggcag aacaggactt agaactagaa aaaattgcta atacctaatt
      541 tgctaatgtg caatttgcta atgtgcaatt tgctaatcaa caaattagcc aactaataaa
      601 tactaactta taaataaata ctaaataaca ataaatacta acagataata agcatcatca
      661 tcaacatcag gaggagctat ggacgaaaaa aagctttttg aaaatttcca attaactttt
      721 ggacggatga tatcgccatt tgaaatcgaa gatattcaaa agtggattca cgaagataac
      781 atgccaattg aagttgtcaa ccttgcctta agagaagcgg tagaaaacaa caaaatcagt
      841 tggaagtata tcaataaaat cttagttgat tggtataaat ctggagatac gacagtagaa
      901 aaggtcagag acaggttgca acggtttgac gatagtaaaa aacaacgaag tgtaactacc
      961 tcaaacgtcc caagctggtc gaatccagac tacaaagaac cagatttaaa agaatttgct
     1021 ctaggaagca tggacggtat agaagatgga tcaggagatt tttaattttt ttaacaaaca
     1081 aatcaaaaaa gattttggta aaacggcgag taaagagact tttgctaagt ttgctagtta
     1141 ctgcgccgaa ggaatcgaaa aaaatggagt taagccaatt tttaattgga taaacctata
     1201 cgcttttgga actggtatga caacagcaga agcagaccgg ttgagaatag agcgatataa
     1261 acaggagaat gcgttatgac aaaacaacat agagaaacgc ttatctggta ccgagcaagt
     1321 catcaagagc gtgagagatt gcttgatttt ggactagttg ataaagcacg gtacgtgaca
     1381 ctattgcggc aattgcgtaa gaaatatgcg atttaggagg aaatatgaca ccagaacaag
     1441 cagaaaaagc aaaaatcaga gctaaacaag aacttgaaac gtttagcata taccttgatc
     1501 aggcaattga tgatctcggt ggagttctaa cttcacgaga agtcttttta gcagcgggaa
     1561 taacatatct tggcgcaggt cagacagata tacatgctgc agtcgaggga ttatgtgagc
     1621 aaatccaatg attttaaatt tagtgaagac tgggagagca actaaaacac aaaaagagga
     1681 aatgaaatga acatcaaaga aaaaattgta gtgctaagaa acactgaaga cggaagtttt
     1741 ttaaagagtt tcaaaaacaa aaaagatgta cttgcttata atgtggaact tacagatagc
     1801 attcaactgg catcattttt accagaagaa gcttacaaca tacaaaaaga aaaaaattga
     1861 taatttggca gaaacgcttg ggtgtgatgt tgtagttatc gaagcatcat atgacctaaa
     1921 atttattgat ggcgaatcag tgccagagtt aacaaaagag caaaaagtta aaagtatggt
     1981 aaacggaatg tttgagcagg tttttggagg tgaatagaga tggcaaatca attatcaaca
     2041 tacacacaca aacaattttt taacgcacca acagttcaaa aagcttttga tgacgtttgg
     2101 aaaggcgccg ggacacagtt cgcggtaagc atcttgtcag tactacaagg tagtcaaagt
     2161 ttgaaatcgg catctaataa atctatttat gcagcagcta tgaaagcagc cgtgttaaat
     2221 ctgcctattg agccaagctt aggaagggcc tatttagttc cgtacaaagg tcaagcacaa
     2281 ttccaattag gctataaagg gttgattgag ttagcgcagc gcagcggaca atacaaaaat
     2341 attaatgcag gtatcgtcta taaatcacaa ttaatttctt acaacccttt gttcgaggaa
     2401 ttaatccttg actttagcaa gccacaagat gaaattgtag ggtattttgc cgcttttaaa
     2461 cttttcaatg gattcgagaa agtttctttt tggacagtag aaaaagtaac tgcacatgga
     2521 aagaaatttt caaaatcgtt tgctagcggt ccttggaaaa cagactttga tgcaatggct
     2581 caaaaaacta ttttaaaaga tattttgagt aaatatggtc cgttatcagt tgagatgcag
     2641 aaagctatcg aggaagataa tcaagattca acgatttcta ccccaaaaga cattacccca
     2701 caagaagcaa atagccttga cgacctaatt ggtcaccaga acgaaaataa ggatgctcct
     2761 aacaatttaa aagacgtaac tgaagattta catgacgaag aagaaaaaac gctcacagac
     2821 gaaaataaga cggttttaga agatacgtct tatccagcag atgaggttcc cgattttgac
     2881 caagagactg gtgaagtatt agctagcgaa ggcaacttat ttgataacct cggagactta
     2941 atatgattaa acttgtaaaa attgaaggat attatattaa tccagaatat gttgttggcg
     3001 tttgggaaag atctgcttta aattttgacg atttagatga aaaagtagtg gttattcaat
     3061 tcgttggaga tagggaaagg gaagagtttg tcttttgcga tacacctatt gatgaagtaa
     3121 ttaagaggtt gctagatgac tagtttagac ttgctcggaa aggactatta cagccgtgaa
     3181 tcagctatca ggtactggtc cattagtcag tacaagcgtt ttaaagagtg cgaagcgcgg
     3241 gcgcttgctg aattacaagg gaattggaca gataccagag ataacactgc gttgctcgtc
     3301 gggaactatg tccactctta ctttgagagt aaaaaagctc atgaagaatt caaagcccaa
     3361 aacggctctg aaatgatttc gacccgagga gcaacaaaag gccaattaaa aaaggattac
     3421 ttagttgctg aacagatgat tgacgctctt aaaagtgata gtaactttat ggctatctac
     3481 caaggagaaa aggaagcagc aatcacagga tttcttggcg agattgaatt caagggtaaa
     3541 atcgactgcc tgaatgttga acgtggctat tttgtagaca tcaaaacaac aaaagggccg
     3601 attgacgaca cgatttggag tggcgaagag cgtgtcagat ggtttgaggc ttacggatat
     3661 atcttgcaga tggctgctta caaaaccatg ttagaagcta aatataataa accgttcgag
     3721 ccgattattt acgcggtaac caaggaaacg ccaccagata caagagccat cagaatccaa
     3781 aatgtagacg ctatgcaaaa cgagttagat agcctagcac aaagcattaa gcgattagat
     3841 gacgttaaaa aaggcataga aaaacctaag ccttgcggtc attgcgagta ttgcagggct
     3901 aatcaattaa cacaaagagt aatgattttt taggagataa aataatgata atagagagga
     3961 ataagcggag gatatggtaa gaagtgagta tgctagagct aaccacatct ctaaagaatg
     4021 ggtagtactt tactagaaaa gtggaggaat ttaaatgatt gaattcgtaa aagaagtagg
     4081 aatggctttg ttatggctgt ttgtaggata tcttgttgga gagcgtagcc aacaaaaaaa
     4141 taagtaaaaa atacgtgccg tgaaccacga aaaaagcgaa ctagaaataa gtgtcattta
     4201 acggtcatgt gactagaatg atgtaaagaa tagcgactgc ccgtatttag ccaaattaac
     4261 aacttttggc agtcgctttt agttttaggg aaatgctatg gatgaattaa aagagaaagc
     4321 attatccaaa atgctagatg aaatgaacaa aaaacatagt gcggctgaag acgccataca
     4381 taattggcta tctgatcagg atgacgaaga actattcaaa ggcatacttg acgataagaa
     4441 atcaattaaa gatgccatga agtactgcat caatcaggca caaaaacaaa aaacaggtaa
     4501 ttgcgccatg gttgacgata aaacagtatt cggttgggtc cgtgtgtatt tcactggaaa
     4561 aaccaaaaag atcgaaccag ttcaagcaac tgtcacagtt agccaagaaa atacgaaagt
     4621 tataccaaag aaaaataaaa aatcaaaagg tgtggttgag ggccaacttg acctatttgg
     4681 tgcgcttgta tgaagaaaaa ccaaaagatt atcgaaggtc gtttaaagcc acctaaggca
     4741 ttctttgact ggtgctatga acaaataccc accatcagat ggcaaaacaa ggaaaagaca
     4801 atcgaaactg aacgtgaaca caaagtctat attgataata ggcgcttgac taagcggact
     4861 agactagatt tctttgataa gttttacagc tttgctattg tccttgtcac ttccaagcga
     4921 attgaaattc aatcctatgg cttttactcc agatatagca atggaagaca atcgattgaa
     4981 gtgaagttgt gtaactttga aaaattggcc aacaatcatc acatcaaatt gactgaacaa
     5041 tttggccagt atgtgcctgg attaacacca aattttatag gctctggagc ctattcaggt
     5101 accaaatttt atcctgatga ttgggaagaa aaaatcaagt caatatcaga actgaagtat
     5161 ttgcaatttc cgatcggctt agcacatcat aacttgcccc acatgtataa gtacagaacg
     5221 caaatagagt tcctacaaaa aatcgatgct tggaagatag caaatgattt ggcttatgat
     5281 gtgactgaat acgatgcttg gcatgtacgg aaagccgtag attgtcgagt gattaccaaa
     5341 aaatggttac accagaacaa gcaattcttc aaaaacagta acaggagttt tagagaatat
     5401 gagcttgagc gtcgaattaa agctagaaat gggaagttgg ttcctggtat tgaaaaaata
     5461 ttgtcttatc aagacatcaa taaaattcca aaagcggcca aaatcaacag gtttcaaaac
     5521 tggttggtta agaataaggt agattttaac tattatatcg attatctgag tatgcttgat
     5581 gaattaggta ttgaaattga cactgataat ctgattatgc caaaagacct agtaaaggcc
     5641 catgataatg ttgtcaaatt agtcaatcaa aagaaaagcg agattgaaaa acgcaaattt
     5701 aaaaatcgtc tcaaaagcct ttctaaatat gagcaaacga ttggcgatta ctgtttcaga
     5761 gcacctgtcg attcaggaga actcatccga gaggggaaaa ctctatctca ctgcgtagga
     5821 agcgcacgat atacacaggc ccacgcttct ggaaaaacaa ctattatttt tatccggaga
     5881 aaatctgatt cagacaaacc attttacaca atggagtata aagccggcca tatcgttcaa
     5941 gtcagaggca aacataatca gtcagctact gaagaagtcc aaaaagtagt tgatcagtgg
     6001 ctggccattg taaacaaaaa ttataaacac gcataggaga aataacatga taacaaaaat
     6061 taatgttcca aaaacatcca tcgtaattga aattgaaaac aaggaaatca cgattgagaa
     6121 tttgattaat tatgatatta aaatgatttt cagaaatcag gataatgaac cgtctcttga
     6181 tgaaaacggg gacatctttg aaccgatgta ctggctagac ataaaagcta aacctaatga
     6241 tgagattgag tatcacagca gcttaggcgt caaaaaagaa aagagaagac tcgctgaact
     6301 acaaatcttc tttgaatata ttgaagcaaa taaacaaaat ctctttgacc tctgtgggct
     6361 aaaaggagaa ctcgcatgag tagcttgaca ttatcacttg atgtctctac tactggaacg
     6421 ggttgggcta tttttgatgg ttcaactcta atccagagcg gggctagtaa accaaaacaa
     6481 aaatcattct atgagcgtgc caaatcaatg gctagcgagc taaaaactat ccaacttaga
     6541 gcgattcaga aacatgataa gccttttgag gacattgtga ttgaacagaa cactgtactt
     6601 ggaccgaatc aacagtcgtc tataaaaatc gggattgcaa ctggtattat cttagggaga
     6661 ttacttgctg aagagattta ttttgtaaat gtatcgacgt ggagaaaata ctggaaattt
     6721 agctataaag accgctcgaa aaaatccatg aagaaacagg caatcaatgc agtagctgcg
     6781 gaatttaata agcaagtgaa ggatgacgaa gctgacgcga tactgattgg gtcttacttt
     6841 actaatcttg gaaaggattt tggcgactta gaaagtcaca aaaattgaga gaataaagtg
     6901 acatataaag tggaggtaat gaaatggaaa ttgtaaaaga tggagttaag tttattgcca
     6961 gtatgataat tgttctggca gctacatttg gctgtggata tctgttttac cgtgcaggat
     7021 acgaacaggc gaaaaaggaa gttcaaccag ttaccatcta taccgtcgat aatgtaggtg
     7081 gtgtaatggt aggagaaatt acggataaag aaattataga gggacggtac acggtcacgg
     7141 ctcatgccta cggtaagttt ctagtaacaa aagaacagta caaggcgata aaagttggcg
     7201 ataaaatacc tgactatttg aagaaaagag gtacaaaatg attgagttaa cacaaaaaca
     7261 ggctgattat gttgagtttg tcagaaaaac tattgtagat ccagatatga tggaattcgc
     7321 ggtaaataca caactgatta gctatgcttt acttgttgga tattgcattg ttgatgaaga
     7381 ggtggccaaa tgaaacctag aaagtatccg tattcaggaa agataagaat tataaaaaaa
     7441 gaattaccaa ggtttgtaag attgggagat tttgctttta atagcaacct agttaaacat
     7501 attgataaaa tcagacaagt aaaaccaaat gaaacgctaa ttcgtttcaa aatccctaag
     7561 ttgtttatga catatgaaga agaaactttt aaagtaagac ttgagattga taaagtcgta
     7621 aaaatattga accaatacta aacaaaaaaa gccagctcac agctgaccat tggttaataa
     7681 ttcgataaat ctattatacc aaaaaggagt taggaagtga gcaaagctaa ggcaatttta
     7741 aaagacttac gcaatttaga tttatatatc gctagtttaa ttagacgtca ggaaaagatt
     7801 gagacctcat tgctttctag ccctaaatgg tcgtccgata aggttaatgg tggtataaaa
     7861 cgtaagcaag atgatgttta cgtagagttg attgcaactg ccgaagatat cgagaagaag
     7921 actgccgaag ctataagaaa acaaagagag cttcagaacc tgattgatag ccttgaaaat
     7981 acagacagtc aaacaatttt aagcatggtc tatattgata agatgactag atggcaagtg
     8041 attgatgagc taaattgcag cgaaagcacc tatttcagac tgttaagagt tgcaactaag
     8101 gaattaaata atttgacagt aaatgacagc gattgacagt gaataacagt gcatgacagt
     8161 tttaaagtga taatatagta ttatcaagaa atgagggaca ggtaagaaaa tacccccctc
     8221 ttttaaattt agaaaggccc cctatgtctc aattaagggc agataaaaaa ggtactcaca
     8281 gggtagcatt tgaaaagaat aagcgaaagc tgttaaagac taccaacctc tgtggtatct
     8341 gtggtaagcc agtcgataag acactgaagt atcctcaccc gcttagcgca gcaatagatc
     8401 acatagttcc tattgcaaaa ggtggacatc cgtcagcgct agagaactta caacttacac
     8461 attggcaatg taataggcag aagtctgaca agttgttcgc taaccaagca agcaacgagc
     8521 caaagacaat aggaaataga aaccttcctc aaagtcgaga ctggtcatct tttacattaa
     8581 aaaagtgaat ttgtgcaaat tattgattta tgctaaaact ggtcagaagc taaacggggg
     8641 gtatggccct ccccggtcgg ccggccgagc ttcacgccgt cactgtacat tttttctcac
     8701 gttaggattt agaaaatttt ggaggttgaa tttcattgaa aaagaaatgc ttaatttgca
     8761 aaaaaaactt ccaagcaaaa accaatagaa ctttatattg ctctgaagaa tgccgtaaga
     8821 aaggcaatcg tgagaaacaa cgtaaattga tgaaacaaaa acgggctgaa cagaggaaag
     8881 aaaaaaagaa agtcctaaat cctaacacag atgtgacaga aaagcctaaa aaaatacgta
     8941 atttagcgca gcactataaa aaactaaaaa aggaaatttt ggcaaatgaa tctgaatttg
     9001 gttttactgg aataacactt attgaaggaa tagatgtaca tgaagaaaac tttgtagatt
     9061 tagtcatgca aaaaataaag gagcagaaat gaattatatg ggtatgggct atcttcgtag
     9121 gaagttagct ctttttaaaa ctggagttga taaaagatat cgttattatg ccatggatga
     9181 cagagacgac acacgaagta ttgtcatgcc aaataatgtg cgtgaaatgt acaggtctgt
     9241 gttagaatgg accgctaaag gggttgatag ccttgcggac cgtattattt tcagggagtt
     9301 taccaacgat gattttaatg cttgggaaat ttttaaagcg aataaccctg atatcttttt
     9361 tgatacagcc atacagtcag cattaattgc atcttgttgc tttgtgtaca tcatgccagg
     9421 agcggaagac ggcttaccta aaatgcaagt tatcgaagct agtaaagcga cggggatact
     9481 tgacccaact acatttttat taacagaggg ttatgcaatt ttagagtctg actcaaacgg
     9541 taatcctacg ttagaggcct atttcacaga caaagacatc tggtattatc caaaaaaagg
     9601 gaaaccatat aatattaaaa atccaacagg tcaccccttg cttgtaccta tcattcacag
     9661 accagacgca gttagaccat ttggtcgcag tcgcattacc aaggctggaa tgtatcatca
     9721 aaaggcagcg aagagaacgc ttgagagagc agaggttacg gctgagtttt actcattccc
     9781 acaaaaatat gttttgggaa tggatccaga cgctgaaccg atggaaaagt ggcgtgctac
     9841 ggtgtcaacg ttactcgaaa tctcaaaaga cgaggatggc gacaagccaa cagttgggca
     9901 attcacaaca gcaagcatgg cccctttcat ggaacacttg aaaatgtatg cttcgctatt
     9961 tgctggtggt tccggtctca ctcttgatga ccttggtttt ccatctgaca acccatcgtc
    10021 tgttgagtca ataaaagcgg cacatgagaa tttgagagca gcaggacgca aagctcaacg
    10081 ttctttttct tctggatttc taaatgtggc gtatattgct gtttgtttaa gagatgaatt
    10141 tccttatctt cgtaatcaat tcatggatac agtaattaaa tgggagcctc tctttgaagc
    10201 tgatgcaaat atgctaactt tagtagggga tggagctatc aaacttaatc aagctatccc
    10261 tggtttcatg gatgcagatg ttatccgtga tttaacggga gtaaaagggg ctgataaacc
    10321 tatacctgct atcacggagg taacaactga tggttgatga tgtcttacct aagctactaa
    10381 aatctgttcg acaagatttt gaaaagtatt ttggcgaaag tgatgttgta accaaggctt
    10441 ttgcagaatt gcaagctaaa aaagtgacct ataaaacagt caatgagttt gctattgaag
    10501 tcggaagact tttatctttg gctctgacag gttctgttag ctcggataaa ttaccagacg
    10561 gtaaaatgta ttacaatatc gctaagcgtc tcttagatga gactatggga cggaattaca
    10621 aattgatttc aggttatgct ggtgatgttc agcggatttt aaatgagaat gctcagattg
    10681 gtctaaaggt gcaacgtcca ccgctaaatc gagacaagat taacgggatg gtaaatcgtt
    10741 tggatagcga gaatacattt gatgatgtaa aatggctgtt tggtgaaccg attgttaatt
    10801 tttcgcagtc cattgtggat gataccatca aggcaaatgc ggatttacaa tataaaacag
    10861 gtatgacgcc acaggttgtc agaacagaaa gtggcaactg ttgtgaatgg tgtcgtgagg
    10921 ttgttggcac ttatagttat ccaaaagtcc ctaaggacgt atggagaaga catcagcggt
    10981 gtcgatgtac gcttgactat gacccgaaga atggaaaagt tcagagtgct tggagtaaaa
    11041 tctggcgaaa gaaagaaaaa actcaagaat ctatagaaag ggttgaaaaa tttaaggaaa
    11101 gtgctctagt agaatctatc aaaaatgata tcgctaagtt agatatgaca aaggttggtc
    11161 cgagtgatat aattgatata gggaaacgta tcaattatca ctttagagtt tcagaacata
    11221 taggagataa agagaaatta aaagaaattt tctcaaattt tcgtgaaatc ggtggagaaa
    11281 tacctaaaaa tacttgggca aaaggttcat ctaaacttgt taaagaccag ctacaagagg
    11341 cttttcaaaa ttatccaact gagtgggcag ctgttccgga cggtattggt aaaaaactaa
    11401 aagctataaa aaggaagcgc ggttacttcg atggatatga tgaagattta gttattgcta
    11461 caaatggaac aagaaaaaca acaccttacc atgagatagg acatatgatt gaactggtta
    11521 atcctgattt agtaagatta gagaaagcct gggtagataa aagaactgct aatgaagctg
    11581 aggttcgctt gaaagatatt tttccaagtt caaattatgg tattggggaa gttactaaga
    11641 aagatgactt tatatcacca tatattggta aatactacag cgatgcagct gaagttttca
    11701 ctatgggatt acaaggtatt tttgttcctg aagaacgatt tgctaagtcc tttgacaaaa
    11761 aaacatggaa atatgactac aagacaatca atgacgaccc tgaatttttg aattttatta
    11821 ttggattatt tgtgaaagtg tgataaatat ggaaccaaaa ttacatcggc aactgcgtca
    11881 aaaatatgac gacgctgaaa aacaatatct tgaaaagttt ggagaagact cgcttgatag
    11941 agtatttttt tgggagccag acgtttactt tgatgagtgg aaaaaggttc taccagatgc
    12001 aacactggaa ttaaacaaag ctattaatag cggggtggcg attgatccag atccagaaaa
    12061 cgcaatatat taagcaccta gagcaatctg gtgcttttct tatgcccaaa aaggagaaaa
    12121 gcatgaataa aatatcataa aatgtcgaaa tgacttggga aaacaaagac gaatttcaac
    12181 aaatcatgaa taaagtcaat gaaacaaaag aagcttacga aaaagcctta gaagctgcgg
    12241 aaaattttgt ccctaaaatg tcgtgtgcaa caaagttaaa taaaggagaa ggtaatgaat
    12301 aaacgtatca agaaaaaacg taaattggaa acagctatta tgctattgat tgcagaaaat
    12361 gccttgcagg ctgaagcaat taaaaatcaa aacaaagaaa tcatggagtt gaaatcaatt
    12421 gttcagcgga acgctctggc aacaaacgaa gagttagcga ctgttaaagc tgctacttta
    12481 gataaccaat cagttatcaa ggcaatcggt gacacggttg actacatcaa gaaaaactac
    12541 aaacgtaagt gggggaagtg atatgaaata tcgtaagaaa ccggtcgtag ttgaagcaat
    12601 tcaatttacg ggtacaaact atgaaaaaat caaagagttt attggtcaaa ataccttatg
    12661 ttcgactttg agtattgtaa tccccactct tgaaggcgac tacatcatca aaggcatcgc
    12721 tggtgaattt tatccatgta aacctgatgt ttttgaacag acttatgaat caacttaaac
    12781 caaagtcgta gcaatacggc tttttattgt gccctgtcgc atggctaaaa actaggcagt
    12841 acgattgaaa ggaataagta tggttactaa gacgaaaaca aagcttggca atcagcgacc
    12901 tactcaatcg gtaaatttac attttgctaa atctctagcg catgaagcta ttaattacta
    12961 caaaaaaaca gggctaagct gctatccatg gcaagtaaat atgcttatcc caattatggc
    13021 catagatgaa aatggtctgt gggttcatca aaagtatggg tatgctatcc cacggcgtaa
    13081 cggtaagacg gaagtagtct atattgttga gctgtgggct ttgcataaag gtttaaaaat
    13141 cttgcataca gctcatcgaa ttagcacatc tcatgcatca ttcgaaaagg taaaaaaata
    13201 ccttgagatg tcaggttatg ttgatggaga agactttata tcaaataaag ccaagggtca
    13261 agagcgtata gagttcaaag ccagcggcgc tgttatccag ttccgaacta ggacatcaaa
    13321 cggtggactt ggtgagggat ttgacttact tatcattgac gaggcacaag aatacacatc
    13381 tgagcaagaa tcagcattga agtacacagt tactgatagt gataatccaa tgactattat
    13441 gtgtggaacg ccgccaacga tggtatctac tggtacagta tttgaagcat atcggaaaga
    13501 ttgcttaaaa ggcaataagc gttattctgg ttgggctgaa tggtcagttc ctgagatggt
    13561 taagattaac gatgtatctt cctggtatat ttccaaccca tctatgggat tccacctcaa
    13621 cgagaggaaa atcgaagctg aattaggtga agatgagatt gatcacaaca tccaacgctt
    13681 aggttattgg ccatccttta accaaaaatc agttatatcc gaaaaagaat gggcaaaact
    13741 caaagttgag caagtgccag aactcaaaag caagcttttt gtcggtatca agtttggtca
    13801 agatggcaac aacgtatcac tatcaattgc agcaagaaca tcagaaaata aggtatttgt
    13861 cgagactatt gactgtttat cagttaggaa tggtactcaa tggattatta attttctgaa
    13921 atcagctgac attgctaagg ttgtaattga tggcgccagc ggccaagaat tacttgctca
    13981 ggagatgaaa gagcaaggtc taaagaaacc agaattgcct aaagttgctg aaattatcac
    14041 agctaacatg atgtgggagc aggggatcat gcaggaaacc atttgtcata gtgatcagcc
    14101 atctttgaca gcagtagtca caaactgtga aaagaggcaa attggctcta atggtggttt
    14161 tgggtataag tcgctttatg atgatagaga cattagctta atggacagtg cattgcttgc
    14221 gcactggatt tgttacacaa cgaagccaaa aagaaagcaa agaaccagct gttaaaaaac
    14281 gacatccgaa agggtgtttt tttactgcta aaaaatctac cgaactgccg ggaaagcagg
    14341 agaaaggacg ttaatatgtc agaatttaaa gttattgaaa cacaagaaga gttggacacg
    14401 attgtgaaag ctcgcattgc tcgagaacgt gagaaatatc aagattacga ccaactgaaa
    14461 actcgtgttg aagaactaga aaccgaaaac agcagcttac aaactgcttt gaatgatgct
    14521 aaatcaaaca ctgatagcta tacggaagag attagcaccc tgaagaatca aattgccgat
    14581 tatgagacgg caaatttacg gacaaaggta gcgttacagt atggcttacc aattgattta
    14641 gctgatcgtt tgcaaggaga tgatgaagat ggactcaaag tagatgcaga acgcttagca
    14701 tcctttatta agccatccca accacaaccg ccagcaaaat caaacgaacc aaatatcgat
    14761 agtaatgcag acgcaaatta cagagcgtta gtgcaaggat taagtacaga agattagaag
    14821 attaattaaa ggagaaaaaa tatgggaaca gaaacatcaa aagcaagctt atttgacaaa
    14881 catttagtat cagatcttat caataaagtt aaggggcata gctcactagc taaactatct
    14941 agccaaaaac ctattccgtt taacggatct aaagaattta cgtttacatt agattctgat
    15001 attgatgtgg ttgctgaaaa cggtaaaaaa acacacggtg gcttatcgct agagcctgtt
    15061 actatcgtac caatcaaggt tgagtatggt gctcgtcttt ctgatgaatt tttatatgca
    15121 acagaagaag aaaagattga tattttgaaa gcttttaacg aagggtttgc gaaaaaactc
    15181 gctcgtggta ttgacctaat ggcgatgcat ggtatcaatc cacgtacaaa aaaagcgtct
    15241 gacgttatcg gcacgaatca ctttgatagt aaagtgacgc aagtggttaa atttacagag
    15301 agcgaagacg ctgatgcaaa tatcgaagca gctgttaatc taattcaggg ggctgaaggt
    15361 gtagtgacag ggcttgcgat ggatacagag ttttcaacag cactggcgaa agttaccaat
    15421 ggggagatgg ggcctaaaat gtaccctgaa cttgcttggg gagcgaatcc agatagcatc
    15481 aatggcttga aatcgtccgt caatactact gttggtgctg gagctgatga agcagaatcc
    15541 aaagatttag taattattgg cgattttgaa agcatgttta agtggggtta tgcaaaacaa
    15601 attccaatgg aaatcattaa atacggtgat cctgataatt cggggaaaga ccttaaaggg
    15661 tataaccaaa tttacttacg tgctgaggcg tatatcggct ggggtatttt ggacgctaag
    15721 agctttgctc gtgtcactaa aggagaagta taatgttata cgttaacagt aagactggag
    15781 ctagttttta tagctccgtc cctgtttgtg gtggagattg gactttggtt gaacagcaga
    15841 caggcgaaaa agcaaaaaca gtagcagaaa ttaaacgtca gttagatgct gctgggataa
    15901 aatatagctc taaagctaag aaacctgaat tagaggcact cttgcctggt taggaggtaa
    15961 aagtgggcaa ttttgcaaca acagatgacg tcattttgtt atggcgtccc ttatctgttg
    16021 acgaattgaa acgtgcaaat gcactcttga aagtcgtatc agatacatta agaatggaag
    16081 ctgacaaagt tggcaaagac ttagataaaa cgatggttga taagccttat tttgttaatg
    16141 ttattaaatc ggttacggtt gacattgtag ctagaacact catgacatct actcaaggtg
    16201 aaccgatgtc gcaagaaagt caatcggcac ttggctacac ttggtctgga acttatttgg
    16261 ttccaggagg gggactgttt ataaaagaca atgagcttaa acggcttggt ctaaaaaagc
    16321 agaggtatgg aggtattgag ctttatgggg aaattaagag ggataacgat tactttgata
    16381 gataaagtaa ctatcgatat agaccctttc ggaaatccaa taaaaaaaga taaagaaata
    16441 tctgtcgata atgtccttgt atcgccagca acaagcgatg acataacaag ccagcttagc
    16501 ctttcaggaa aaaaagctgt ttacacttta gctattccaa aaggagacaa ccacgattgg
    16561 ggagataagg aagttagatt ctttggagaa aagtggcgca ctgtcggcct agctcttgaa
    16621 ggtattgaag agcttattcc gcttgaatgg aataagaaag ttatggtgga aagatatgag
    16681 taagtttaaa ttcaagctca ataaagctgg tgttgctgaa ttgatgaaat catcagaaat
    16741 gcagcaggta ttaaccacta aggccacagc catcagagaa cgttgtggtg atggttacgc
    16801 ccaagatatc catgtcggga aaaatagggc taatgctatg gtcagtgcta aaaccataaa
    16861 ggccaagaaa gataattcaa aaaacaacac attgttgaag gcggtgcgat gattgattga
    16921 agtaattatc aaaaaatatt tagacgagca cttagatgtg ccgtcttttt ttgaacatca
    16981 aaaagatgaa cctgcacgat tcatcatctt agaaaagact agcggggcta agcaaaatca
    17041 tttgctaagt tccacgtttg cttttcaaag ttatgccgaa tcgttgtatg aggcggcttt
    17101 acttaatgac aaagtaaagc aagtaattga gcagcttgat gtcttgccac aagtttctgg
    17161 tgtacatctt aacgctgact acaattttac agatacagca actaagcgtt atcgatatca
    17221 agctgtattt gacattaatc actattaaca aaaggagata ataatggcaa atgatactaa
    17281 aaatgtaaca tctgcaaagc caaaagcagg cggagctatt tattctgcac cgttggggac
    17341 aacattgccc gaggatgcaa aatcaaaatt agatactaaa tttaagaatt tgggttatgt
    17401 atccgaggat ggtgtggtca atgaagatac acgctcatct gaaaatatta aagcgtgggg
    17461 tggagatatt gttggatctg tgcagacaga aaaagaggat aaatttactt ataagctgat
    17521 tgaatcacta aatgtagaag tcttaaaaga agtttatggc gctgccaatg ttactggaga
    17581 tttagataag ggaatccata ttaaatcaaa ttcaaaagaa cttgaagctc atgctatcgt
    17641 tattgatatg atcatgaatg gtggaattct gaagcgcata gttttaccga atgcaaaagt
    17701 cgatgaggta ggtgaaatta agtatgttga cggcgaagtt gtcggatacg aaacaacact
    17761 aaaatgtttt ccagatgaaa aaggagatac tcatcatgag tatattgtaa aacctggaga
    17821 agctaataaa aaagaaaaca gctttgaaat gtaaagggga gtaaatggaa atcttaaaag
    17881 gaaaaacaac atcaggattt gaatacgaaa tccctaaaaa acgattaaaa aactttgaac
    17941 ttgttgaagc tattgcagaa gaggaaaccg atccaacagc agtggttaaa atcgttaatt
    18001 tgttacttgg tgatgctgct aagtctctaa aagaacatgt acgagatgca gaaggtatcg
    18061 tagacgttga agctatcgga gtagaaatca aagaaatttt tgaaagtcaa aaagatttaa
    18121 aaaactagca atcctcgctc agatgatagc aaaagatgat gatgcattga cttgtgattt
    18181 agctgaaaca tacggcatat atgattacag acagctacct gcttatcagg tggctgtttt
    18241 tgctgtcggt ttgagatcta actctaggat aaaaatggca ttatctggag agactgaggc
    18301 tttggatact gttttgttag ctggtattta cgataatact aatttgctgt tttggtctaa
    18361 aactaaggat ggtcaatctg gtcaaaacaa acctaaatcc gtggtggaag ctatatctgg
    18421 atctaaatca caaaaagcta atgatgtcat ttcttttgcg tctggcgagg attttgaaaa
    18481 tgcacgtaaa caattactag gaggtgatgg ctaatggcaa cagaacttgg tcaagcgtat
    18541 gtgcaaatta tgccatccgc tcgtggaata agtggagcaa tctcaaagca acttgatcca
    18601 gaagcaaggt cggctggttt gagcgcaggt aaattgtttg gtagtaaatt gaaattagcg
    18661 gctctagcgg ctactgctgg tttaggtatt ttactcaaga aaacaatttc tgcatctttg
    18721 tccactggtg ctgacttaca gcaatctttt ggtggtattg ataccttgta taagggcgct
    18781 gagactgctg tcaaagggtt tgctaaagaa gcgtacaaag ctggtatatc agcaaatact
    18841 tatgcagagc aagcagtttc aatgggtgca tctctaaagc aatcacttgg aggtgatgct
    18901 gtcgcggctg ccaaggctgc taacatggca atcatggata tggccgacaa ctcggctaag
    18961 atgggtactg atatcacatc aatccaaatg gcttaccagg gatttgctaa gcaaaactat
    19021 acaatgcttg ataacctaag acttgggtac ggcggcacaa aagaagagat gaagcgtctt
    19081 ttatcagacg ctgaaaagtt acctgccgct atgggcaaga agtttgattt gagtaattat
    19141 gctgatgtgg ttgaggctat acacttggta caggataaca tgggtatcgc tggagttgct
    19201 gctgaagaag caaaaactac attttcaggc tcactagctg ctatgaagtc ctcttttaca
    19261 aatgtactgg caggtttatc actaggagat gatataagac ctgctttacg aggactggct
    19321 gaaacaactt ctaatttctt atttggtaac tttattccga tggtggcaaa tatctttaaa
    19381 ggattaccat cggcaattgg tacttttatt ggagcagcaa ctcctattat tgccagtcaa
    19441 tttaaagggc taatgagtag ccttgggatt agtattgatt taagtcctat tactgctaaa
    19501 tttgcacaga ttggccaaaa tttacaacct gtttttaacg gtttaaaaac ggcttttagt
    19561 cagttgccat cattttttac tagcattggt agtgcagttg caccagtaat agacactatt
    19621 attagcggat tagccagatt agatttcagt ggttttgagg ctttaatttc agcaatctta
    19681 ccagcaattc aaacagggtt ccaaacgttc atgtctattg taggtccagc gattagtcag
    19741 cttgtaaata gttttgttaa tctttggaat gcttgccaac ctctagtaac aatattaagt
    19801 ggcgcattga tgccagcttt tcaaatttta ggagcttttc tcggcggagt cttacaagga
    19861 atccttggca gtattagctt tgcatttgat gctttaaaag ttgttattga gtttttaacg
    19921 ccaattattg attggcttgt acaaggtttt aatgctattt caccggcatt acaaatcgtt
    19981 gcccagtggg ttggtaatgt cattggtata tttactagct taggtgcatc tgggcaaggt
    20041 ttaagtagta tgctaagcag tgcttggggg aacatccaaa cagtagtttc gacagctaaa
    20101 aatatgataa ctttggcgat tgatggtatt aaattggtat ttagcaatct agggaatgct
    20161 ggaaatattt taaaaggatt actttcggca gcgtggtcag ctatgcaaaa tgcagtagtg
    20221 atagctaaag gtattatcaa cagtgcgata agcgctataa aaacagcatt tagcagcttt
    20281 ggtaatcttg tatctagcgt aagtggaact ataaaatctg ttattggcag tttaaaaaat
    20341 gcgttttaca gtttagcaag tattgactta gttggtgcag gacgtgcaat catgcaagga
    20401 tttctaaatg gactaaaatc gatgtggggg gcaatcaccg gctttgttgg gggtattgct
    20461 gactggatcc gtaagcacaa agggcctatc tcatacgata gggttttgct aaaacccgca
    20521 ggtaaagtaa tcatgcaagg gttcaacaat agcttgatgg atggctttaa agaggttaaa
    20581 tcaaatgtat ctggcatggc tgacgatctt gcaggcacca taacaggtaa aagtctatct
    20641 ctcggtatcg atgctaaacc aagcgtcaca gctgatgact tactatcaag caatattagt
    20701 actaaaacta cagtcggttc tgctacaagt gacttgtcat tattttttgt taaggtgctt
    20761 gctctgttgc aagatatcct tgataaaaat acggatgtct atctagacaa agaaaaagtc
    20821 agcgctattt tatacgaaga atttgccaaa atcatggcta gagaggggat tgcatgatac
    20881 caaaggttat tattgatggt tttgatacct ctacaatccc taattgtgtc ctaactggtt
    20941 atgatgtagg ggatgtttta tcccctcatt ttgtcgaaaa tgaagtgtat gggatgaatg
    21001 gtactagtag gggattagag tcatacaatg agtcgaaacc cactctaaca tggcatttga
    21061 gcacttttga tgatgcagtt aatttagtga atcatttaga cggacttggg aaaatcattg
    21121 aattttggca tattccgaag tcattttatt attatgattg cctatctgtc aaaatcaatg
    21181 cggtaactat gaactcgtgg agagtcacac ttaaacttgc actatatcca tttaggtatg
    21241 cgaaaggtat ctcagatgta acgattgcag gcaatggaag tataaataat ccaggaaatg
    21301 tttttagtga acctaagata attgttgagg gtaccggcaa aggaacacta accattgaca
    21361 aacaggtcat ggaattaaat ttgtcaggta aagcaacgat tgagtgcaaa catggccaac
    21421 aatgcgttta tgatggtgaa ggtaatgtga aaaactcaat ccgaataaga ggaagatttt
    21481 ttgaaataca acctggcaca caaggtattg ccgtaagcgg aggcattgcc aggttaacaa
    21541 ttagtccaag gtggaggtac aaggtttgat atcgattaaa gatgataata cacctcttgt
    21601 agcagccttt gaagatgaga ttacacagga agcgaatagc gattataaat taagttttaa
    21661 gtatccagct aaacatgagt atcgcccctt gatcaaaaaa ggaataatcc tagaagctga
    21721 tgacctacat ggaactcagc tttttagggt ttttgagatt actaagcgac atggctatat
    21781 caatgtttat ggtaatcagg tcgccgatga tttaaatggc tatgcaattg actctatcag
    21841 tgttgatagg gtgcaaggta tgacagtaat gtcagagtta gcaggtaata tcaagcgtga
    21901 gcatcctttt agctttttta gtgatattga cggtcgtcac acatttaatc aatcagacgt
    21961 atctgttatg gacgctttag ctaatggcaa gcactcaatc atggggcagt ggggtggcga
    22021 gcttgtacgt gataagtatc aggttaattt gctcaaaaaa gctggtaaag acactgagac
    22081 cttatttatg tataagaaaa acctcaaatc ttatgaggag acagatacta taaaaggtct
    22141 catctcgata ctgcatctcg ttgcagaagt tgaggagcag cgggaagagg aatctagaga
    22201 agtttctgat gcagacattg gccacaatga agttagcaaa aagaaaacga ttagggtaac
    22261 tgttgagagc aagctcaaag acactcatcc gataattgtt gaaaagacta tcaaggtgca
    22321 ggatcaagat gtcaaaacag aagaggactt gcttgcatat ggtaagaaat actttgaaaa
    22381 aactctttgc gacataccag gtaatagttt aaaaattgat gttactaata actacgaggg
    22441 cgctgttagg ctatttgaca cagcaattgt cttccacgag ctctatgaca gagacttacg
    22501 aatgcaaatc actggctatc ggttcgcccc tatggccaat cggttaaaat ccatcatctt
    22561 tggagagatt aagaccaact tagcaaaaca aattagcaat caaattgaca ataaggtagc
    22621 tgaatcaact gctcaacatg acgcagcatt tgaagcaaaa ttacaaaagc agattgataa
    22681 tgctaatcgt atttttgaca caaaagaagc taaactccgt gaagagattg aagatggcat
    22741 caaaaaagct gaagctaatg cagaggtcaa agttgctgag gttaacgcta aagtgctgga
    22801 agctgaggag ctagccaagg cagtcgatga
//
LOCUS       DAUCLR010000025        22742 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.025, whole genome shotgun sequence.
ACCESSION   DAUCLR010000025 DAUCLR010000000
VERSION     DAUCLR010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22742
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.025"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(188..385)
                     /locus_tag="VGT87_000787"
                     /pseudo
     CDS             complement(188..385)
                     /locus_tag="VGT87_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001883004.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(419..625)
                     /locus_tag="VGT87_000788"
     CDS             complement(419..625)
                     /locus_tag="VGT87_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ3813663.1"
                     /translation="MTQITLKAARINAGYTLKQVAGAVGKNPQTISKYEKDSSDISLG
                     LLQKLSSLYGVTIDNLFLGKKVRK"
     gene            779..1546
                     /locus_tag="VGT87_000789"
     CDS             779..1546
                     /locus_tag="VGT87_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ3813664.1"
                     /translation="MAKNSPQDLINREIFSTNLNMLMAKKNIKQIDIHNKLGIPKSTI
                     TGYVKGRSLPTAGNVQKLADFFGVLKSDIDPRFDSNNIETNSNIIPSTLQKVTSTLSQ
                     LEHKRQLNVLDYAETQLEQQNTVEEPQATYYTYNYYDHAASAGTGQYLNDVQVETIEL
                     PVDYDADFVIPVYGDSMEPEYHSGDYVFIKLSINLSDGDIGVFEYYGDAYIKQLVIND
                     SGAFLHSLNDKYDDILIDRDSDFRIIGEVIGSFTSKS"
     gene            1937..2098
                     /locus_tag="VGT87_000790"
     CDS             1937..2098
                     /locus_tag="VGT87_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813665.1"
                     /translation="MSINKKQVFLTLCGISISVTGIDMLLSGEYLRGVIKLILAFGIF
                     VIFSKDRDE"
     gene            2359..3213
                     /gene="dinD"
                     /locus_tag="VGT87_000791"
     CDS             2359..3213
                     /gene="dinD"
                     /locus_tag="VGT87_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HEQ3813666.1"
                     /translation="MNGLQHYDELAFENIKHIDENGVEFWYARELQTVLEYAKWDNFL
                     NVIERAKIACENTGNPVAMNFPEVSKIVQLPLGDREIKDYKLSRYACYLIVQNGDPRK
                     EVIALGQSYFAIKTRQQELADNFNKLDEEHKRLAIRQEMKEHNKSLAEAAKMSGVTNY
                     GKFQNFGYKGLYGGMSMQDIHDRKELEKGQSILDYMGSAELAANLFRATQTDEVLRNR
                     QIHDENLANDTHFNVGRTIRKTMQKLGTTMPENLPTPQESIQDLKKKHKQLDKQPDDN
                     QLSLFDDM"
     gene            3471..4616
                     /locus_tag="VGT87_000792"
     CDS             3471..4616
                     /locus_tag="VGT87_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEQ3813667.1"
                     /translation="MKIKEHKKKNGAIVYRASIYLGIDQMTGKRVKTSITGRTQTEVK
                     QKPKHAQFDFLSNGSTIKRKVVIKTFKELSHLWLETYKLTVKPQTYDATVTRLNRHIM
                     PTLGNMKVDKITASDIQMLINRLSKYYVNYTAVRSVIRKVLQQGVLLGLIDYNPARDI
                     ILPRKQPNAKKKVKFIDPSDLKSFLEHLESSQHKRYNLYFDAVLYQLLLSTGLRIGEA
                     CALEWGDIDLENGTIAINKTYNKNLKFLSTAKTQSGNRVISVDKKTLRSLKLYQMRQR
                     QLFNEVGARVSEVVFATPTRKYFNASVRQSALATRCKEAGIERFTFHAFRHTHASLLL
                     NAGISYKELQYRLGHANISMTLDTYGHLSKDKEKEAVLYYEKAMNNL"
     gene            4711..6693
                     /gene="mutL"
                     /locus_tag="VGT87_000793"
     CDS             4711..6693
                     /gene="mutL"
                     /locus_tag="VGT87_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEQ3813668.1"
                     /translation="MTNIIELPEVLANQIAAGEVVERPASVVKELVENAIDAKSSQIT
                     VEIEESGLKMIQVTDNGEGMSHEDLTLSLRRHATSKIKSQSDLFRIRTLGFRGEALPS
                     VASISKITIKTATKEVTHGSLLIATGGEIETLEAISTPTGTKIKVENLFYNTPARLKY
                     MKSLQAELAHIVDVVNRLSLAHPEVAFTLISDGRQLTQTSGTGDLRQAIAGIYGLNTT
                     KKMLAISNADLDFEVSGYVSLPELTRANRNYMTILVNGRYIKNFLLNRAILDGYGSKL
                     MVGRFPIVVIDIQIDPYLADVNVHPTKQEVRISKERELMALISTAISESLKEQDLIPD
                     ALENLAKSSTRHFSKPEQTQLPLQSRGLYYDPQKNDFFVKESAVSEKIPETDFYSGAV
                     DNSVKVEKAELLPHSEEVIGPSSVKHASRPQNIFTETDHPNLDLKNRQKLSQMLTRLE
                     NEEKSVFPELDYFGQMHGTYLFAQGKDGLFIIDQHAAQERVKYEYYRDKIGEVDSSLQ
                     QLLVPYLFEFSGSDFINLQEKMALLNEVGIFLEVYGHNTFILREHPIWMKEEEIASGV
                     YEMCDMLLLTNEVSIKTYRAELAIMMSCKRSIKANHSLDDYSARNLLLQLAQCQNPYN
                     CPHGRPVLINFSKADMEKMFRRIQENHTSLRELGKY"
     gene            6704..7924
                     /locus_tag="VGT87_000794"
     CDS             6704..7924
                     /locus_tag="VGT87_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ3813669.1"
                     /translation="MQEFLNLPKQIQLRQLVRFVTITLGSSIFPFMAMYYTTYFGTFW
                     TGLLMMITSLMGFVGTLYGGHLSDALGRKKVIMIGSVGTTLGWFLTILANLPNAAIPW
                     LTFAGILLVEIASSFYGPAYEAMLIDLTDESNRRFVYTINYWFINIAVMFGAGLSGLF
                     YDHHFLALLVALLLVNVLCFGVAYYYFDETRPETHAFDHGKGLLDSFRNYRQVFHDRA
                     FVLFTLGAIFSGSIWMQMDNYVPVHLKLYFQPTAVLGFQVTSSKMLSLMVLTNTLLIV
                     LFMTVVNKLTEKWKLLPQLVVGSLLFTLGMLLAFTFTQLYAIWLSVVLLTFGEMINVP
                     ASQVLRADMMDHSQIGSYTGFVSMAQPLGAILASLLVSVSHFTGPLGVQCLFVVIALL
                     GIYFTVVSAKMKKV"
     gene            7926..8522
                     /gene="ruvA"
                     /locus_tag="VGT87_000795"
     CDS             7926..8522
                     /gene="ruvA"
                     /locus_tag="VGT87_000795"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEQ3813670.1"
                     /translation="MYDYIKGQLTKITAKYIVVEANGLGYMINVANPYSFTDSVNQLV
                     TIYLHQVIREDAHLLFGFHTEDEKDVFLKLISVSGIGPTTALAIVAVDDNEGLVNAID
                     NSDIKYLMKFPKIGKKTAQQMVLDLAGKFVEAPQETGHTKARSNKAGNTQLDEAIEAL
                     LALGYKAKELKKIRAFFEGTSETAEQYIKSALKLLMKG"
     gene            8532..9092
                     /locus_tag="VGT87_000796"
     CDS             8532..9092
                     /locus_tag="VGT87_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEQ3813671.1"
                     /translation="MKRCSWVPKDNQLYCDYHDLEWGQPLHDDRDFFELLCLESYQSG
                     LSWLTVLKKRQAFRTVFHHYDIASVATFTSEELADALENPSIIRHKLKLAATVNNAIA
                     VQKIQEEFGSFSTYLWNFVGGKPINNLVNQENLVPAQTELSIRLAKDLKKRGFKFLGP
                     TTVYSFMQASGLVNDHEEACVFNRIF"
     gene            9161..10432
                     /locus_tag="VGT87_000797"
     CDS             9161..10432
                     /locus_tag="VGT87_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEQ3813672.1"
                     /translation="MKAELIAVGTEILTGQIVNTNAQFLSEKMAELGIDVYFQTAVGD
                     NEERLLSVITTASQRSDLVILCGGLGPTKDDLTKQTLAKYLRRDLVYDEQACQKLDDF
                     FAKRKPSSRTPNNERQAQVIEGSIPLPNKTGLAVGGFITVDGISYVVLPGPPSELKPI
                     VNEELVPLLSKQYSTLYSKVLRFFGIGESQLVTVLSDFIENQTDPTIAPYAKTGEVTL
                     RLSTKTENQALADKKLGQLEAQLLSRKTLEGQPLADVFYGYGEDNSLARETFELLVKY
                     DKTITAAESLTAGLFQSTLASFPGASQVFNGGFVAYSMEEKAKMLGLPLEELKSHGVV
                     SAYTAEGMAEQARLLTGADIGVSLTGVAGPDMLEEQPAGTVFIGLATQNKVESIKVLI
                     SGRSRLDVRYIATLHAFNMVRKTLLKLENLL"
     gene            10521..11657
                     /gene="recA"
                     /locus_tag="VGT87_000798"
     CDS             10521..11657
                     /gene="recA"
                     /locus_tag="VGT87_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEQ3813673.1"
                     /translation="MAKKLKKNEEITKKFGDERRKALDDALKNIEKDFGKGAVMRLGE
                     RAEQKVQVMSSGSLALDIALGAGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLSASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGTTQIKGTGDQKDSSIGKETKIKVVKNKVAPPFKVAE
                     VEIMYGEGISRTGELVKIASDLDIIQKAGAWFSYNGEKIGQGSENAKRYLADHPQLFD
                     EIDRKVRVKFGLLEESEEESAMAVASEETDDLALDLDNGIEIED"
     gene            11948..12346
                     /gene="spx"
                     /locus_tag="VGT87_000799"
     CDS             11948..12346
                     /gene="spx"
                     /locus_tag="VGT87_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEQ3813674.1"
                     /translation="MIKIYTISSCTSCKKAKTWLNAHKLAYKEQNLGKEPLTKEEILA
                     ILSKTENGVESIVSSKNRYAKALDCDIEELSVSEVIDLIQDNPRILKSPILIDDKRLQ
                     VGYKEDDIRAFLPRSIRNIENTEARLRAAL"
     gene            12460..12729
                     /locus_tag="VGT87_000800"
     CDS             12460..12729
                     /locus_tag="VGT87_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEQ3813675.1"
                     /translation="MGFTDETVRFKLDDGDKRQISETLTAVYHSLDEKGYNPINQIVG
                     YVLSGDPAYVPRYNDARNQIRKYERDEIVEELVRYYLQGNGIDVK"
     gene            12726..13145
                     /gene="ruvX"
                     /locus_tag="VGT87_000801"
     CDS             12726..13145
                     /gene="ruvX"
                     /locus_tag="VGT87_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEQ3813676.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIKIDEEKAEFGFTR
                     LEELVKQYQVEQFVIGLPKNMNNTNGPRVDASITYGNHIEHLFGLPVHYQDERLTTVE
                     AERMLIEQADISRGKRKKVIDKLAAQLILQNYLNRNF"
     gene            13157..13462
                     /locus_tag="VGT87_000802"
     CDS             13157..13462
                     /locus_tag="VGT87_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEQ3813677.1"
                     /translation="MTHNHENDHQHEVITLVDEQGNETLFEILLTIDGREEFGKNYVL
                     LVPAGSEEDESGEIEIQAYSFTENEDGTEGDLQPIPEDSDAEWDMIEEVFNSFLDEN"
     gene            13875..15434
                     /locus_tag="VGT87_000803"
     CDS             13875..15434
                     /locus_tag="VGT87_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HEQ3813678.1"
                     /translation="MIELNDEFIRKETIELANDGPRVHTTQYETKVPRLHKCYLLFFS
                     IIISSLTIAVPFLTDAANGLQSQNLYIGMMLTKGQLPYSDAFTTGGLFYFVIIALSYY
                     LGSTLWLVFVQVFCFYLSGLYLYKLINYMTGFQKVALTFSISYYLLSVSLGFGGLYPT
                     QLAMPFILISAWFLTKYFACLVKDEAFILFGFVGALAMLIDPSTLIFWSFACVTVFSY
                     NISQKHLARGFYQLLASIFGMILVFYTAGYFILNLQVLNPYLSQTMIYPFTFFKSGNL
                     SLLFGLAIQLFFALGLGLLTGMENVIRRFKNNSDRVVKWLFVMVILESILVAIFSQDY
                     RPYHLLPLLPFGLILTAIPVGYQYGIGLGQSSHRRRHGKNGVGRVMMIYLKRHFYLPI
                     LIVGTILICSTYCFISSIPLNQERDHIASYLEQKLNKTQSIYVWDDTSKIYLDSKAKS
                     VSQFSSPDINTQKASHRKILEDELLENKAAYIVVNRYKNLPKIIQKVLSTNYKVDKQI
                     TTKSFIVYQKK"
     gene            15531..17729
                     /gene="nrdD"
                     /locus_tag="VGT87_000804"
     CDS             15531..17729
                     /gene="nrdD"
                     /locus_tag="VGT87_000804"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEQ3813679.1"
                     /translation="MVSLEEDKVTVQPDIKVIKRDGRLVNFDSTKIYSALLKASMKVT
                     RMSPLVEAKLEAISDRIIAEIIERFPTNIKIYEIQNIVEHKLLAANEYAIAKEYINYR
                     TQRDFARSQATDINFSIDKLINKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLKM
                     LPSHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLANGFKIGNAEVESPKSIQT
                     ATAQISQIIANVASSQYGGCTADRIDEFLAPYAELNFKKHMADAKKWIVETKRESYAF
                     EKTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSWFEREIQKAILTIRINGL
                     GSEHRTAIFPKLIFTVKRGLNLEPDSPNYDIKTLALECATKRMYPDMLSYDKIIDLTG
                     SFKSPMGCRSFLQGWKDENGQDVTSGRMNLGVVTLNLPRIAMESNGDMDKFWELFNER
                     MLISKDALIYRVERVTEAKPANAPILYQYGAFGKRLEKTGNVNDLFKNRRATVSLGYI
                     GLYEVASVFYGGQWEGNPDAKAFTLSIVKAMKQACEDWSDEYGYHFSVYSTPSESLTD
                     RFCRLDTEKFGIVTDITDKEYYTNSFHYDVRKSPTPFEKLDFEKDYPEAGASGGFIHY
                     CEYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDKCYNCQFEGDFTPTERGFTCPNCG
                     NNDPKTVDVVKRTCGYLGNPQARPMVNGRHKEISARVKHMNGSTIKYPGL"
     gene            17911..18057
                     /locus_tag="VGT87_000805"
     CDS             17911..18057
                     /locus_tag="VGT87_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813680.1"
                     /translation="MGKYQLDYKGMQQVERFHEKHSKKKTDKKSRVQELKARFLEKSK
                     KQDN"
     gene            18086..19021
                     /locus_tag="VGT87_000806"
     CDS             18086..19021
                     /locus_tag="VGT87_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEQ3813681.1"
                     /translation="MLNIGIVGLGAISQKAYLPYMRQLSDITWHLSTRNAAVRQQVGQ
                     LFGHAILYSDVKELSKTNLDGVFIHAATSAHAELASLFLNQGIPVFMDKPIADNYLMT
                     KNLYDLAKENQTFLMAGFNRRFTPRVKKLSSLSTKRKVAVEKNDLNRPGDMTFKLFDF
                     FIHPLDTALFLTEGTLLKGHFQYHLEAGLLSQVMVTLMTESMTTTASMNLQSGSRREV
                     MEVQRAEETYHLENLDELSIYKGTEKRVLSFASWDTTLHKRGFETMINAFLEAISTGV
                     NPVSPESSLLSHWICQQIADSQLSYGELTVELPKD"
     gene            19030..19539
                     /locus_tag="VGT87_000807"
     CDS             19030..19539
                     /locus_tag="VGT87_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ3813682.1"
                     /translation="MEIRRPTLKDKDAVLSMINEFLEQKSATDGLWHFNVNDFNYETW
                     LEDSLRQEMGLSSQGVPAIQYVAFDERSQAIGFLNLRLRLNERLLEKGGHIGYSVRPS
                     RRGKGYAKEMLKQAVSYAISKNITTILVTCDETNVASRAVIVANGGILEDSRGGTERY
                     WIEEQRGDK"
     gene            19539..20153
                     /gene="nrdG"
                     /locus_tag="VGT87_000808"
     CDS             19539..20153
                     /gene="nrdG"
                     /locus_tag="VGT87_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEQ3813683.1"
                     /translation="MAEKCWNNPKPKEWQAEELSQGRIIDYKAFNFVDGEGVRNSLYV
                     SGCLFHCKGCYNAATWSFKAGMPYTQELEEQIMTDLAQPYVQGLTLLGGEPFLNTGIL
                     IPLIKRIRRELPEKDIWSWTGYTWEEMMLETPDKLEMLSLIDILVDGRFDITKKNLML
                     QFRGSSNQRIIDVQKSLAAKEVIIWDKLNDGDQTFEQISREDLL"
     gene            complement(20327..21058)
                     /gene="yaaA"
                     /locus_tag="VGT87_000809"
     CDS             complement(20327..21058)
                     /gene="yaaA"
                     /locus_tag="VGT87_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEQ3813684.1"
                     /translation="MLTFLIPTAKEMMIPKESHPHLLPQDSQAILKIMAAMTTEDLAK
                     SYRIKEEAAKKEQQRWQDMASQQSLAYPAYQLFNGLMYRHIKRDKLTTQEQAYLTQQV
                     YITSSFYGIIPANHPIAEHRHDFHTRIKIEGQSLKSYWRPCYNQFAKEHPQVISLLSS
                     EFDDVFSKDCKQLWISPKFMAEKEGQFKTHSTISKKARGAFLTACMENNCQTVDSLKS
                     LVFAGFYYHPDLSTDHEFVYIKKEA"
     gene            complement(21100..21957)
                     /locus_tag="VGT87_000810"
     CDS             complement(21100..21957)
                     /locus_tag="VGT87_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEQ3813685.1"
                     /translation="MTSPYNSTISLGTVSLNVTDLAKMTTFYTSIISLQVLSQDTTSR
                     QLTTDGKTVILELRQTPLPANKAYGLYHTAFLVPDRHSLGLVLNHFLTRGISLEGSAD
                     HGYSEAIYLSGPEGNGIEIYHDKAVEHWDIRDNGQIIGVTEPTDTKSILEQLTDIPKR
                     FLLAQDTRIGHVHLSVKNALASSLLYQKVFDLGDKMTIPSASWIASGNYHHHLAFNHW
                     SAPYLKKHQEGAPGLAFLTIHIETPLLFSATLKKARLHGLAILQEDSSSFTTEDEEGI
                     CVNVILGQD"
     gene            22144..22481
                     /locus_tag="VGT87_000811"
                     /pseudo
     CDS             22144..22481
                     /locus_tag="VGT87_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT         7152 a         3791 c         4758 g         7041 t
ORIGIN      
        1 tagcttgtac gtcatagtac ccttggctag tttttgtgta atcaatcatt tcagcgactg
       61 ccctagccaa aaataaaggc tcctgaatat caccgtaaat gtctaaaggt tgtccatgaa
      121 aatttgtcgt tgtaataact tgcataacct gctttgtgtt gtcattagct tcttcaaaaa
      181 tatttacttg tgtcatcata gtttctttca cctctctata tagcctattc agtttctttc
      241 tgctttcttg gttcgggtta tgtttaccct gccaccattt accaactgtt cgggggtcta
      301 ttccgatacg ttgaccaata gcaactaaat tcaagttgta ggaagttctc agctcatcta
      361 tgaccttgct ataattcctt ctcatttctt gcctgccttt ctaattaata aagtactatt
      421 attttcgtac ttttttacct aaaaaaaggt tatcaatagt gacaccgtac aaagatgata
      481 atttttgcaa tagtcctaaa gaaatatcgg agctatcttt ttcgtacttt gaaatagttt
      541 ggggattttt cccaaccgct ccagctactt gttttaaagt gtaaccagcg ttgattcggg
      601 cagcttttag tgtaatttgc gtcatgtttt caactccttt ctaaaaagct atggcttaat
      661 agtactatta ttttcgtacc aagtcaatga tttttgtaga aaaatattaa aaaaatagta
      721 cttttaggtt tatttgtgtt agaattaatt cagatacaaa aagaaagggg taagagaaat
      781 ggctaaaaat agtccccaag atttaataaa tagagaaatt ttctcaacaa atctcaacat
      841 gcttatggct aaaaagaata tcaaacagat agatattcac aacaaactag gaatacctaa
      901 gagtacgata actggctatg ttaaaggtcg ttcactccca actgctggaa acgttcaaaa
      961 gctggcggac ttcttcggag ttctaaaatc agacattgac ccccgttttg attctaataa
     1021 tattgaaaca aatagtaata ttatcccatc aaccctacaa aaagtaacat ctactttatc
     1081 tcagctagaa cacaagcgac aattaaacgt ccttgattat gctgaaacac aattagaaca
     1141 acaaaacaca gtagaagaac cacaagccac ctactacact tacaattact acgaccacgc
     1201 agcttcagct ggtacaggtc agtatctaaa tgatgtacaa gtagaaacaa ttgaattacc
     1261 agtcgattac gacgctgatt ttgtcatacc ggtttatggc gattctatgg aacccgaata
     1321 ccattctggg gactatgtat ttatcaaact atctattaac ctgtcagatg gtgatatagg
     1381 agtttttgag tattacggtg atgcttatat caaacaactt gttataaacg attctggagc
     1441 atttctgcat agtctgaacg acaagtatga cgatatactc atagatagag atagtgattt
     1501 ccgtattatc ggagaagtta ttgggagttt tacatctaaa tcatgactat ctgataccca
     1561 cgcgccgaat tccactattt tccactaatt ttggttgctt tacctttaag gactcgcatt
     1621 ttgctcttta agacagccga aaagtccgat tttctgaata ctatatgcta aaaatgccaa
     1681 ctgattttag aagctgtcac aacggaaaaa gtaaattaat aaacgaccga tatatcaagt
     1741 tctttaagtg aatttaccga gcgttttaga actattgaaa taggttgacg tattatgtcg
     1801 gtacatacca acattttcca gcattccgaa atgcgaccat gtgttcgtgt ttgggagtag
     1861 taaccttcta ttctctaagt tcgctcgaag ttcagcatta tgcgcgtggt tgtatttcaa
     1921 taaaaggaga ttttctatga gtataaacaa aaagcaagta tttttgacac tgtgtggtat
     1981 ttctatttca gtgacaggta ttgatatgct tctatctggt gagtatctaa gaggagttat
     2041 caagttgatt ttagcttttg gaatttttgt catatttagc aaagatagag atgaatagcc
     2101 catagtttct attttaagtt cgaaaatgcc cactataatg tgttaaaaca gacatgaaat
     2161 actctagcag tgtaaaacta tactccactg ctttaagtct aaaaatgagc taccagagta
     2221 gaaaaaacga cttataacta aaactattat cttatctgtt aagcagttgc ataaaatatc
     2281 ataaaaatat acataaaatt atttttattc atttttaact caccgtacct tgaccgttag
     2341 accatagaaa ggaaaagcat gaacggatta caacattatg acgaattagc tttcgaaaat
     2401 attaaacaca tagatgaaaa cggtgtagag ttttggtatg cccgtgaatt acaaactgtt
     2461 ttagagtatg ctaaatggga caatttttta aacgttattg agcgggctaa aatagcttgt
     2521 gaaaacactg gaaaccccgt cgctatgaat tttcccgagg tcagcaaaat tgtacagctc
     2581 cctctaggag atagggagat taaagattat aaactatctc gctatgcctg ctatttgatt
     2641 gtccaaaatg gtgaccctag aaaagaagtc attgcactgg gtcaatcata ttttgctatt
     2701 aaaacccgtc agcaagaact agctgataat ttcaacaaac tcgatgaaga gcacaaacgt
     2761 ttagcgattc gccaagagat gaaggaacat aataaatcat tagctgaagc tgctaaaatg
     2821 tcgggtgtca ctaactacgg caagtttcaa aactttggat ataaaggcct ttatggtgga
     2881 atgtctatgc aagatattca tgatagaaaa gaactagaaa aagggcaaag tattcttgat
     2941 tatatgggta gtgctgaatt ggccgctaac ttattccgtg ctactcaaac cgatgaagtg
     3001 ttaagaaatc ggcagataca cgatgaaaat ctagctaacg atacacactt taacgtaggt
     3061 agaacaatca gaaaaacaat gcagaaacta ggcacaacta tgcccgaaaa tctccctaca
     3121 cctcaagaga gtatccaaga tttgaaaaag aaacataaac aactagataa acaaccagac
     3181 gataatcaac tttcactttt tgatgatatg taaaaaatag gcaataatat cctcgtatag
     3241 ctatttcaac tctcaatcat ataattttat caacttaccc cccaaaaaaa aaacaaaaaa
     3301 gaccccgcaa gttttccacg ctcgcaaggt cttaaaaagc ctaatattat accatgattt
     3361 tcttttataa tatttcggat atttacccga taccattatt ataccatgat atgaactaat
     3421 ctaaaaccct tttaataata gcttgcctgc tgatggaaag gtttataatc atgaaaataa
     3481 aagaacataa gaagaaaaac ggtgcaatcg tttatcgtgc tagtatttat ctaggcattg
     3541 accaaatgac aggtaagaga gtaaaaacaa gcatcacagg aagaacacaa acagaggtca
     3601 agcaaaaacc caagcacgcg cagtttgact tcctgtctaa tggatctaca ataaaaagaa
     3661 aagttgtgat taaaacattt aaagaactta gtcatttatg gcttgaaacc tataagttaa
     3721 cagtaaagcc tcaaacttat gatgctactg ttactagact taatcgacat attatgccaa
     3781 ctctgggcaa tatgaaggtt gataagataa ccgctagtga tattcaaatg ctaattaata
     3841 gattatctaa atattatgtc aattatactg cggtacgttc agtcatccga aaagttctcc
     3901 aacaaggagt attgctaggg ctaatagatt acaacccagc aagagatatt atccttccaa
     3961 ggaagcagcc aaacgctaag aaaaaagtta agtttattga tccgtctgat ttgaagtctt
     4021 ttttagaaca tttagaatct agtcaacaca aacgctataa cctttacttt gatgcagttc
     4081 tctaccaact tttattatcc actggcttga ggataggcga agcctgtgca ttagagtggg
     4141 gagatattga cctagaaaat ggtacaatag ccattaataa gacttacaat aaaaatttga
     4201 agtttttgag tacagctaaa acccagtctg gtaatagagt gattagtgtt gataaaaaga
     4261 cccttagaag cctaaagctc tatcaaatga gacagcgaca attatttaat gaggttggtg
     4321 cgcgtgtgtc ggaggtagtg tttgccacac caacacgaaa gtattttaat gcttcggtta
     4381 gacaaagcgc tttagctact aggtgtaagg aagcagggat tgaacgcttt acctttcacg
     4441 cttttagaca cactcacgct agtttattgc tgaacgctgg tattagttat aaggaacttc
     4501 agtaccgtct aggacatgcg aatatcagca tgactttgga tacctatggc catctttcta
     4561 aggacaaaga aaaagaagct gttttatatt atgaaaaggc tatgaataat ttataagtcc
     4621 acaaaaaagt ccacaaatta atattttgag aggtgtaaac ctaatgaaac cttgttatat
     4681 caacgtttag aaagtgttaa aaacaaaatt atgacaaaca ttattgaatt accggaagtt
     4741 ctcgccaacc aaattgcagc tggtgaagtt gtagaaaggc cagcgagtgt tgtcaaggaa
     4801 ttggttgaga atgctattga tgctaaaagt agccagatta ccgttgaaat tgaagagtct
     4861 ggccttaaga tgatacaggt tacagacaac ggtgagggaa tgtctcatga agatttaact
     4921 ttaagtctgc gtcgccacgc tactagtaaa attaagagtc agagtgattt gtttagaatt
     4981 agaacacttg gcttccgtgg agaggcttta ccgtctgttg cctctatcag taaaatcacg
     5041 ataaaaacag caacaaaaga agtcactcac ggttctcttc ttatagctac tggtggggaa
     5101 attgagacac ttgaagcgat ctcaactcct actggaacca aaattaaggt tgaaaacctt
     5161 ttttacaaca cgcctgctcg tctcaaatac atgaaaagtt tacaggcaga attagctcac
     5221 attgtagatg tggtcaaccg gttgagtttg gcacatccag aagttgcttt cacactgatt
     5281 agtgatggtc gccaattaac tcagacatca ggaactggcg atttacgcca agcaattgca
     5341 gggatttatg gtttgaatac taccaaaaaa atgctggcta tctctaatgc tgatttggat
     5401 tttgaagttt ccggctatgt tagcttgcca gagttgacac gcgccaaccg taattacatg
     5461 acgattttgg ttaatgggcg ttacatcaag aatttcttgc ttaatcgagc aattcttgat
     5521 ggttacggtt ctaagctcat ggttggacgt ttcccaattg ttgtgattga tattcagatt
     5581 gatccctatt tggccgatgt caatgttcat cccacaaaac aagaggttcg tatttcaaaa
     5641 gagcgtgagt tgatggcttt aattagtacg gcgatttctg aaagtcttaa ggaacaagat
     5701 ttgattccag atgctctgga aaacttagcc aaatcaagca cacgacattt ctctaaacca
     5761 gagcaaacac aacttccttt acagtctagg ggactttatt atgatcctca aaagaatgac
     5821 ttttttgtca aagagtcggc tgtctcggaa aaaatacctg aaactgattt ttattccggc
     5881 gctgttgaca acagtgtaaa ggttgaaaaa gcagagctgt tgccccactc agaagaagtt
     5941 ataggacctt cttcggttaa acacgcaagt cgtccccaga atatctttac cgagactgat
     6001 catcctaatc ttgacttaaa aaacagacaa aagttatctc aaatgctaac ccgtttggaa
     6061 aatgaagaaa aatcagtatt tcctgaattg gattattttg gtcagatgca cggaacttat
     6121 ctctttgctc agggaaaaga tggtttattt ataattgacc aacacgctgc ccaggagcgg
     6181 gttaaatatg aatattatcg tgataagata ggtgaggttg acagtagttt acagcaattg
     6241 ttggtgccct atttgtttga gttttccggt tcggatttca ttaacttaca ggagaaaatg
     6301 gcactcttaa atgaagttgg tatcttctta gaagtttatg ggcacaatac tttcattttg
     6361 agggagcatc ctatctggat gaaagaagaa gagattgcat ctggtgtcta tgaaatgtgt
     6421 gacatgttac ttctaaccaa tgaggtatct attaaaactt accgagcaga gttagctatt
     6481 atgatgagtt gtaaacgatc tatcaaggca aatcatagct tggatgatta ttcagcaaga
     6541 aatctgctac tgcaattggc ccaatgtcaa aatccttata actgccctca tggtagaccc
     6601 gtattgatta attttagtaa ggcagatatg gaaaaaatgt tccgtcgaat tcaagaaaat
     6661 cacactagcc tgcgagagct aggaaaatat tagagaggat gagatgcaag agtttttaaa
     6721 ccttcctaag cagattcagc tgaggcaact ggtacgcttt gtgaccatta ccttaggtag
     6781 tagtatcttt ccctttatgg ccatgtatta tacgacttac tttggtacgt tttggacagg
     6841 cctcttaatg atgattacca gtttgatggg atttgttgga actttatacg gtgggcattt
     6901 gtcagatgct cttggtcgca aaaaagtcat tatgattggg tcagtaggaa caacgctagg
     6961 ctggtttttg actattttag ctaatttgcc taatgcggct attccttggt taacctttgc
     7021 gggtatttta ttggtagaga ttgcttctag tttttatggt cctgcctatg aagctatgtt
     7081 gattgatttg actgatgaga gtaatcgtcg atttgtttac accatcaatt attggtttat
     7141 caatattgcc gtcatgtttg gtgcagggct atctgggctt ttttatgatc atcatttttt
     7201 agccttgtta gtagctttat tactcgtcaa tgtactttgt tttggcgttg cttactacta
     7261 ttttgatgag actagaccag agacacatgc ttttgatcat ggaaaaggct tgctggatag
     7321 ttttcgaaat tatcgtcagg tgtttcatga tcgtgccttt gtcttgttta ccttaggtgc
     7381 catcttttct ggtagtatct ggatgcagat ggataactat gtgccagtcc atttgaaact
     7441 gtattttcag ccaacggctg tgttaggttt ccaagtaact agttctaaaa tgttatcatt
     7501 aatggtttta actaatacat tgctgattgt ccttttcatg acagtagtaa ataaattaac
     7561 ggaaaaatgg aaactattac ctcagcttgt ggttggttct ttactattta ctctagggat
     7621 gctcttggca tttaccttta cgcagctcta tgctatctgg ttatcagttg ttttgttaac
     7681 ttttggggaa atgataaatg ttcctgctag tcaagtccta cgtgctgata tgatggatca
     7741 ttcccaaata ggatcttata caggttttgt gtcaatggca caacccctag gtgctatttt
     7801 ggctagtctc ctagtatctg tcagccattt tacaggtcct ttaggcgtgc aatgcttatt
     7861 tgtagtcatt gctttgctag ggatttattt tacggttgtt tctgcaaaaa tgaaaaaggt
     7921 gtaatatgta cgattatatt aaaggtcaat tgaccaaaat tacggcaaaa tacattgtcg
     7981 ttgaagctaa tggattgggc tacatgatta atgttgccaa tccttatagc tttacagata
     8041 gtgtcaacca attggtaacc atttatctgc atcaagtgat tcgtgaggat gctcacctgt
     8101 tgtttggttt tcacacggaa gatgaaaaag atgtttttct gaaattaatt tctgtatcag
     8161 gtattggtcc gacaacagct cttgctattg tggcagttga tgataatgag ggacttgtga
     8221 atgccattga taacagtgac attaagtacc taatgaaatt tcctaaaatt ggtaaaaaaa
     8281 cagcgcagca aatggttctt gacttagctg gcaaatttgt ggaggctcca caagagactg
     8341 gtcataccaa agcgcgcagc aataaagcag gcaatactca actggacgaa gcgattgaag
     8401 ccctccttgc cctcggttat aaagcaaaag agctgaaaaa aattcgtgcc ttctttgagg
     8461 ggacctctga gacggcagag caatacatca aatcagcatt gaaactgtta atgaaagggt
     8521 aggtgtttca catgaaacgt tgctcatggg ttcctaagga taaccagctt tactgtgatt
     8581 accacgattt agaatggggt caacccctac atgatgatag agattttttt gaattactct
     8641 gccttgaaag ttaccagtca ggcttatcct ggctgactgt tttgaaaaaa cgccaagctt
     8701 tcagaactgt ttttcatcat tacgacattg catcggttgc aacctttacc tcggaggaac
     8761 tggctgacgc tttagagaat ccatccatca ttcgccacaa gctaaagcta gcagcaactg
     8821 tcaacaatgc tatcgcagta cagaaaattc aagaagagtt tggtagtttt tcaacttatc
     8881 tctggaattt tgttggggga aagccaatta acaaccttgt caatcaagag aatcttgttc
     8941 ctgctcaaac agaattatct attcgcttag ctaaggattt aaagaaaaga ggatttaaat
     9001 ttttagggcc tacaacggtt tattctttta tgcaagcatc gggtctggta aatgaccatg
     9061 aagaggcttg tgttttcaat agaatcttct aattcttcgt gaattggaag attttttgat
     9121 aaaatgagat cagattacga atagataagt aggtggggaa atgaaagctg aactgattgc
     9181 agtaggtacc gaaattttga ctggtcaaat tgtgaatacc aatgctcaat ttctgtcgga
     9241 aaaaatggca gagctaggta ttgatgtcta ttttcaaacg gctgttgggg acaacgagga
     9301 gcgtttactt tcagtgatta caactgctag tcagcggagt gatttggtca ttttatgcgg
     9361 tggccttggt ccaacgaaag atgatttaac caaacaaact ttagcaaagt accttaggag
     9421 agacttggtt tatgatgagc aagcttgtca gaaactagat gacttttttg ctaagcgcaa
     9481 gccttcatca cggacaccaa ataatgagcg acaggcacaa gtgattgaag gatcaatccc
     9541 tttgccaaat aaaactggtc ttgcggttgg tgggttcatt acagtcgatg gtattagtta
     9601 tgttgtctta ccgggtcctc caagtgaatt gaagccgata gtaaatgaag aattggtacc
     9661 acttctgtca aaacaataca gtacattgta ttcaaaggta ctacgctttt ttggtattgg
     9721 ggaaagtcag ttggtaacag tcttgtcaga ttttattgag aatcaaactg atccaaccat
     9781 tgctccgtat gctaagactg gcgaagtgac tcttcgctta tcaacaaaaa ctgaaaacca
     9841 agctctggca gataaaaagt taggtcagct agaagcgcag ctactatccc gaaaaactct
     9901 tgaaggtcaa cccttagctg atgtctttta tggctatggg gaggataatt ccttagcgcg
     9961 tgagacattt gagctcttag taaaatatga taagacaatt acagcagcag aaagtctaac
    10021 cgcgggatta tttcagtcaa ctttggcgag ttttccagga gcttctcaag tattcaatgg
    10081 aggctttgtg gcttatagca tggaagaaaa agcgaaaatg ctaggccttc ctttagagga
    10141 gttgaaatcg catggcgttg ttagtgctta tacagccgag gggatggcgg agcaagcaag
    10201 gttattgact ggtgctgata ttggggtaag tttaacaggt gttgccggac cagatatgtt
    10261 ggaggaacag cctgcaggta cagttttcat tggccttgcc actcaaaata aggtagaatc
    10321 aataaaggtt ttgattagcg ggcgaagtcg tttggatgtg cgctatatcg ccactttaca
    10381 tgcctttaat atggtccgta aaactttatt aaaacttgag aatttgctat aatgttattt
    10441 agtagctata gtaggatatg gtttacaatt atgtagatta atcctgctga cgatggtttg
    10501 ttaaaaaaga ggagaaagta ttggcaaaaa aattaaaaaa gaatgaagaa atcactaaga
    10561 agtttggcga cgaacgtcgc aaagcccttg atgatgcttt gaaaaatatt gaaaaagatt
    10621 ttggtaaggg cgcagtgatg cgattaggag aacgtgcaga gcaaaaagtt caggttatga
    10681 gttcaggaag tctagctctt gatattgcgc ttggagctgg tggttatcct aaaggacgta
    10741 tcatcgaaat ctatggtcca gagtcttccg gtaaaacgac tgtggcttta catgctgtag
    10801 cacaagctca aaaagaaggt ggaatcgcag cctttatcga tgccgagcat gcgcttgatc
    10861 cagcttatgc tgctgcgctt ggggttaata ttgatgaact cctcttgtct caaccagatt
    10921 ctggagaaca aggacttgaa attgcaggta aattgattga ttctggtgcg gttgatctgg
    10981 ttgttgtcga ttcagtagca gctttagtgc cacgtgctga aattgatggt gatattggcg
    11041 atagccatgt cggattgcaa gcacgtatga tgagtcaggc catgcgtaaa ttatcagctt
    11101 ctattaataa aacaaaaact atcgctatct ttattaacca attgcgtgaa aaagttggtg
    11161 tgatgtttgg aaatcctgaa acaacaccag gtggtcgagc tttgaaattc tatgcttctg
    11221 ttcggctgga tgtgcgtgga acaactcaaa ttaaaggaac tggtgaccaa aaagatagca
    11281 gcattggtaa ggagaccaaa atcaaggttg ttaaaaacaa ggtcgctccg ccatttaagg
    11341 tagcagaagt tgaaatcatg tatggggaag gtatttctcg tacaggggag cttgtgaaaa
    11401 ttgcttctga tttggacatt atccaaaaag caggtgcttg gttctcttat aatggtgaga
    11461 agattggcca aggttctgaa aatgctaagc gttatttggc cgatcatcca caattgtttg
    11521 atgaaatcga ccgtaaagta cgtgttaaat ttggtttgct tgaagaaagc gaagaagaat
    11581 ctgctatggc agtagcatca gaagaaaccg atgatcttgc tttagattta gataatggta
    11641 ttgaaattga agattaaaaa gagcccttca gggcttttta aaatgtattt ttaaaggtta
    11701 gtaggactac tgttcatagg tgtgtgggtg aatagaaacc atcatttaaa aagatgtgag
    11761 agagcagaac ttaggttaac tgatataaaa ttaaaataac taataattca agggaataca
    11821 ggtgaaaaac cttattttac taagataata tctttaaaat taaaaaaata ggtccaaaga
    11881 cctatattta tagataagaa actgtgctac aataaagcta tcacgaaata gaaagtgagt
    11941 cagcaacatg attaaaattt acacgatttc aagttgtacg agctgcaaga aagccaaaac
    12001 ttggctaaat gcccataagc ttgcttataa agaacagaac ttaggaaaag aaccgctaac
    12061 taaagaagaa attttagcaa ttttatcaaa aaccgaaaat ggagtggaga gcattgtttc
    12121 atctaaaaat cgttatgcca aagctctcga ttgcgatatt gaagaattaa gtgttagtga
    12181 ggttattgat ttgattcaag ataacccgcg catccttaaa agtcccattt tgattgatga
    12241 taaacgtctg caagtaggct acaaagaaga cgatattcgt gcttttttac ctcgctctat
    12301 tcgtaatatt gaaaatactg aagcacgatt acgtgctgca ctctaaaagc tcttgtagag
    12361 ctttttttta aataccttct tcgcttgaaa ttcttgttta tttaatagaa ataagctata
    12421 ataaagcata gcacttaatt ttagaaagaa ggtgtaagta tgggatttac agatgaaaca
    12481 gtccgcttta agttggatga tggcgacaaa cgacaaatta gtgaaacatt aacagcagtc
    12541 tatcattcac ttgatgaaaa gggttataat ccaattaatc aaattgttgg ttatgtcttg
    12601 agtggggacc cagcttatgt tcctcgttat aatgatgctc gaaatcagat tcgtaaatat
    12661 gaacgtgatg aaattgtaga agaacttgtt cgctactatt tacaaggaaa tggaattgat
    12721 gttaaatgag aataatggga cttgatgttg gctcgaaaac ggttggtgta gctattagtg
    12781 atcctcttgg ctttacagcc caaggacttg aaattatcaa gattgatgag gaaaaagcag
    12841 aatttggctt tacacgttta gaagagcttg ttaagcagta ccaagttgaa cagtttgtta
    12901 ttggattgcc aaaaaatatg aataacacca atggtccacg tgttgatgca agtataactt
    12961 atggaaacca catagagcac ctctttggac ttccagtcca ttatcaagat gaacgattga
    13021 cgactgttga ggcagagcgt atgcttattg agcaagctga cattagtcgt ggtaagcgaa
    13081 aaaaagtcat tgataaatta gctgcacaac tcattttaca gaactattta aatcggaatt
    13141 tttaaggaga taaaaaatga cacataatca tgagaatgac caccaacacg aagttatcac
    13201 acttgtggat gaacaaggaa atgaaacctt gtttgaaatt ttattaacaa ttgacggtcg
    13261 tgaagagttt ggtaaaaatt atgttctctt ggttccagcg ggatctgaag aagatgagtc
    13321 tggtgaaatt gaaatccaag cctattcatt tactgaaaat gaagatggta ctgaagggga
    13381 tttacaaccg attcctgagg attcagatgc tgaatgggat atgattgaag aggtatttaa
    13441 tagttttcta gatgagaact aaattgttag tgacactatg ttaaaaagta gccgtgaggg
    13501 ctattttttt aatgttattt tcgaataaat aagcaggagg tgataatgat aaaagaatta
    13561 aatgataaat ttattaataa ttgtaaggct aaacctttgc atagatttag agaattatta
    13621 cagtatgtga aaatatataa gaaattgact aaataaaaga ttctgttata cttgttttat
    13681 aatattcttt gacgataata tcatttttca acttactttg tttatgcact gtcactttgc
    13741 tatgtgcaat acaaagtcat gttatttatc tcatactaaa aaataatgga taatagctat
    13801 gttagactag tgatatgaag aaatattgct tttattgaat cgttagagaa tatgatattt
    13861 caaggagaaa aatagtgatc gagttaaacg atgaattcat ccgaaaagag acgatagaac
    13921 tggccaacga tggcccacga gtacacacca ctcaatatga aactaaagta cctcggcttc
    13981 ataaatgcta tctgcttttc tttagcatta ttatcagttc tttaacaatt gcagtacctt
    14041 ttttaacaga tgcagccaat ggtcttcagt cccaaaactt atatatagga atgatgttaa
    14101 cgaaggggca acttccctat agtgatgctt ttacaacagg agggctcttt tactttgtta
    14161 tcattgcatt aagttattat ttgggatcga cactttggct agtctttgtt caggtgtttt
    14221 gtttttactt atctggttta tatctttata aactcattaa ttatatgaca ggctttcaaa
    14281 aagtggcttt aactttttca attagctact atttgttgtc tgttagtctt ggttttgggg
    14341 gattgtatcc tactcaactg gctatgccat ttatattaat atcggcttgg tttttaacta
    14401 agtattttgc ctgtttagtg aaagatgagg catttattct ttttggcttt gtaggtgctc
    14461 ttgcaatgct aattgacccg agtaccctta tcttttggtc ttttgcttgt gtgacagttt
    14521 tttcttataa tataagccaa aagcatcttg caagaggttt ttatcaactg ctagcttcga
    14581 tttttgggat gattttagtt ttttacacag caggatattt cattttgaac ttacaagtgc
    14641 taaatcctta tttatcacaa acgatgattt atccttttac tttttttaaa tcaggaaact
    14701 tatcgttgct ttttggactg gctattcagt tgttcttcgc tttggggctt ggtcttttga
    14761 cgggaatgga gaatgtcatt aggcgattta aaaacaattc tgatagggtc gtcaagtggc
    14821 tatttgttat ggtcattcta gaatctatac ttgtggctat attttcacaa gactatcgcc
    14881 cctatcatct tttacctctt ttaccttttg gattaatttt gactgctatt cctgttggct
    14941 atcagtatgg tataggatta ggtcagagta gtcatcgcag acgtcatggt aaaaatggtg
    15001 ttggtcgagt aatgatgatt tatcttaaga gacactttta tttgccaatt ttaattgtag
    15061 ggacaatact aatctgttct acttattgtt tcattagtag tattcctctt aatcaggagc
    15121 gtgatcatat tgctagttat ttagaacaga aactaaataa aactcaatct atttatgttt
    15181 gggatgatac ttctaaaatt tatttggaca gtaaagctaa atctgtttct caatttagtt
    15241 ctcctgacat caatacgcaa aaagcgagtc atcgaaaaat attagaagat gaactattag
    15301 aaaataaggc tgcttatatc gttgttaatc gctataaaaa cctgcctaaa atcattcaaa
    15361 aagtattatc tactaattac aaagtagata aacagataac gacaaaaagt tttattgttt
    15421 atcagaaaaa gtaatctaat atcaatatat tgtgttcgag caaaaaactt gacacaatat
    15481 attgattttt ttagttgtcg tgatataata gttccgtaag gagatttgat atggtaagtt
    15541 tagaagaaga caaggtgact gttcaacctg atattaaagt gattaaacga gatggtcgcc
    15601 ttgttaattt tgatagtaca aaaatctata gtgctttatt aaaagcaagc atgaaagtaa
    15661 ctcggatgtc gccacttgtt gaggctaaat tagaggctat ttctgatcgc attatagcag
    15721 aaattattga gcgttttcca actaatatca aaatttatga aatccaaaat attgtagagc
    15781 ataagcttct tgcagctaat gaatatgcta ttgcaaaaga atacattaat tatcgtactc
    15841 agcgtgactt tgcacgttca caagcaacag atatcaattt ttctattgat aaattaatta
    15901 ataaagatca aacagttgtt aatgaaaatg ctaacaaaga tagcgatgtt tttaatactc
    15961 aacgagattt aactgctgga atcgtaggga aatcgattgg tttaaaaatg ttaccttcgc
    16021 atgttgctaa tgctcatcaa aaaggagata tccattacca tgatttggat tacagtcctt
    16081 atacaccgat gacgaactgc tgtttaattg actttaaggg catgttagcc aatggcttta
    16141 aaattggtaa tgctgaagtg gaaagtccca agtctattca aactgcaaca gctcagattt
    16201 cacagattat tgcgaatgta gcatcaagtc agtacggcgg atgcacagct gatcgcattg
    16261 acgagttttt agccccatat gcggagctta actttaaaaa acatatggct gatgctaaga
    16321 aatggatcgt tgagactaag agagaaagct atgcttttga aaagactcaa aaagatattt
    16381 atgatgcgat gcagtctttg gagtatgaaa ttaatacgct ctttacgtct aatggtcaaa
    16441 caccatttac ttctttagga tttggtttgg ggacgtcttg gtttgaacgt gagattcaaa
    16501 aagctatttt gaccattcgg attaatggtc ttggtagtga acatcgcacg gctattttcc
    16561 ctaaattaat tttcacggtt aaacgtggct tgaatttaga accagattca ccaaactatg
    16621 atattaagac tttggcttta gaatgtgcga ctaagcggat gtacccggat atgttatctt
    16681 atgataaaat tattgatttg acaggatctt tcaaatctcc aatgggatgc cgctctttcc
    16741 ttcaaggctg gaaagatgaa aatgggcaag atgtgacctc aggccgtatg aatcttgggg
    16801 ttgtcaccct caatttacct cgcattgcca tggaatcaaa tggcgatatg gataagtttt
    16861 gggagctgtt taatgagagg atgctaatta gtaaggatgc tttaatttat cgtgtcgaac
    16921 gtgtcacaga agcaaaacca gcaaatgccc ctattcttta tcaatatggt gcttttggaa
    16981 agcgtttgga gaagacaggg aatgtaaatg atctctttaa gaatcgtcgt gcaacagtct
    17041 ctcttggcta tattggtctt tatgaagtgg cgtctgtttt ttatggtggt caatgggaag
    17101 gtaatccaga tgctaaagct tttaccttgt caattgtcaa ggcaatgaaa caggcctgtg
    17161 aggattggtc agatgaatat ggttatcatt tctctgttta ttcgactcca tcagaaagtt
    17221 tgacagatcg cttttgtcgt ttagatacgg aaaaatttgg cattgtgaca gatattacgg
    17281 ataaagaata ctatacaaat tcttttcact atgatgtgcg taagagtccg acaccttttg
    17341 aaaaattaga ttttgaaaaa gattatccag aagcaggtgc ttcaggtggt tttatccact
    17401 actgtgagta tcctgttttg caacaaaatc caaaggcctt ggaagcggtt tgggactatg
    17461 cttatgatcg tgtggggtat ttgggaacca atacgcctat tgataaatgc tataattgcc
    17521 aatttgaagg tgattttacc ccaacagaac gtggttttac ttgcccaaac tgtggcaata
    17581 atgaccctaa aacagttgat gtggtcaaac gtacatgtgg ttacttgggg aatcctcagg
    17641 cccgcccaat ggttaacggt cgccataagg aaatctctgc gcgtgtaaaa catatgaatg
    17701 gttctactat aaaataccca ggcctgtaaa ctcaagctgg gggagagccc ttttgatgga
    17761 aatatgatat gcttaaaagg ttgcttcatg caagcaatct tttattgttt aatggtttct
    17821 tagcaattgt gagataagaa taactaatcc aaaacgacta attagctgtt agcaatcgga
    17881 ctataagaag aaagagataa ggaagaaata atgggaaaat atcaattaga ctataaagga
    17941 atgcagcagg tagaacgctt tcatgagaag cattctaaaa aaaagacgga taaaaaatcc
    18001 cgcgttcaag aacttaaggc tcggttttta gagaagtcaa aaaaacagga taactaaagt
    18061 atccaataaa gaaagaaggt tgagtatgct taatattggt attgtgggac taggtgccat
    18121 ttcacaaaaa gcctatttac catatatgag acagctgagt gacattactt ggcatctatc
    18181 aacgcggaat gcagcagtac gtcagcaggt cggtcagtta tttggtcacg ctattcttta
    18241 cagtgatgtc aaggagttgt caaaaacaaa cttagatggg gtctttattc atgcggccac
    18301 atcggcccac gcagagttgg ctagtttatt tttaaatcag gggatcccag tctttatgga
    18361 taaacctata gctgacaatt acctgatgac aaagaacctt tatgacttag ccaaagaaaa
    18421 tcagactttt ctgatggcag gatttaacag gcgttttaca cctcgtgtca agaagttgtc
    18481 aagtttgtca actaagcgta aagtggctgt tgaaaagaat gacttgaacc ggccaggaga
    18541 tatgactttt aagctctttg atttctttat ccatccttta gatacagcct tattcttgac
    18601 agaagggacc ttactaaaag ggcattttca atatcatctg gaagcaggct tactgagtca
    18661 agttatggta accttgatga ccgaaagcat gacgactaca gcttctatga atttacaatc
    18721 agggagtcgc cgtgaggtaa tggaagttca acgggctgaa gaaacttatc atttagagaa
    18781 cttagacgag ttatctattt ataaaggtac tgaaaaaagg gtgcttagct ttgcttcttg
    18841 ggacactacc ttgcacaaaa gaggttttga aacaatgatc aatgcctttt tagaagctat
    18901 tagcacaggc gtcaatcctg tcagccctga gtctagtctt ttaagccact ggatttgtca
    18961 acagattgct gactctcagc tttcttatgg agagttaacg gtagagttgc ctaaggatta
    19021 ggaattgata tggaaataag acgaccgact ttaaaagata aagacgctgt attgtcaatg
    19081 attaacgaat ttttagagca aaaaagtgct acagatgggt tatggcattt taacgtcaat
    19141 gactttaact atgaaacatg gttggaagat tccttgcgac aggaaatggg tttgtccagt
    19201 caaggagtgc ctgctattca atacgtcgca tttgatgaga gaagtcaggc tatcgggttc
    19261 ttaaatcttc gtttacggtt aaatgagaga ttacttgaaa aaggtgggca tattggttac
    19321 tctgtccgtc ccagccggcg tgggaaaggt tatgccaagg agatgcttaa acaagctgtc
    19381 agctatgcga tttccaagaa tatcacgaca attttggtaa cttgtgatga gactaatgtt
    19441 gcaagccgag cagttattgt ggctaacggc ggtatcttgg aagatagtcg aggtggaacg
    19501 gaacgttatt ggattgaaga acaaagaggc gataagtgat ggcagaaaag tgttggaata
    19561 atcctaagcc aaaggaatgg caggcagagg agttaagtca aggccggatt attgactata
    19621 aggcttttaa ctttgttgat ggtgaagggg ttcgtaattc cctttatgtg tctggttgtt
    19681 tattccattg caaggggtgt tataatgctg cgacttggtc ttttaaggcg ggaatgcctt
    19741 atacgcaaga acttgaagag cagataatga cggatttggc acagccttat gttcagggac
    19801 tgacactttt aggtggagaa ccctttttga atacgggtat cttaattcct ctgattaagc
    19861 gtattcggcg tgaattgcca gaaaaagata tttggtcttg gacgggttat acttgggaag
    19921 aaatgatgct tgaaacacca gacaaactgg aaatgttgtc cttaattgat attctggtag
    19981 atggtcgttt tgatatcaca aaaaagaacc tcatgttgca atttagaggc tcttctaacc
    20041 agcgaattat cgatgttcaa aagtctttgg ctgctaaaga agtgattatc tgggataagt
    20101 taaacgatgg ggaccaaacc tttgaacaaa ttagtcggga ggatttgctt taagaagctg
    20161 acttatccac atgatttttt tgaaaaggtc atgtgaaaac aggtccttta agggtgaatt
    20221 ggggagaaag taattgttat ttcctggttt tccccacatt atcctcatag ttatccacag
    20281 gttgtgtata agttgtagaa ttgtgaataa agactaaaaa agaggcctag gcctcttttt
    20341 tgatatagac aaattcatga tctgttgata ggtctggatg gtagtaaaaa ccagcaaaga
    20401 caaggctttt aaggctgtca acagtttggc aattattttc catacaggca gtgagaaatg
    20461 cacctcgtgc cttttttgaa atggtggaat gggttttgaa ttggccctct ttttctgcca
    20521 taaacttggg acttatccac aattgtttac agtctttgga aaagacatcg tcaaattcac
    20581 tggataaaag ggaaatcact tggggatgct ctttggcaaa ctgattataa cagggccgcc
    20641 agtaagactt gaggctttgt ccttcaattt ttattctggt gtgaaaatca tggcggtgct
    20701 ctgcgatagg atgattagcg ggaatgatac cgtaaaagga tgaggtgatg taaacttgct
    20761 gggtgaggta agcctgttct tgggtagtga gcttatcgcg cttgatatgg cggtacataa
    20821 ggccattgaa cagttggtag gcagggtaag ccaagctttg ttgagaggcc atgtcttgcc
    20881 agcgttgctg ttctttttta gctgcctctt ctttgatacg ataggatttg gccaagtcct
    20941 ccgtggtcat agctgccatg atttttaaaa tggcttggct gtcttgtgga aggaggtgtg
    21001 ggtgagattc cttagggatc atcatttctt ttgcggttgg aattaaaaag gttagcatag
    21061 tgttattgta gtaaagttac ttgctttctg tcaagaaaac tagtcttgtc caaggatgac
    21121 gttcacacag ataccttcct cgtcttctgt cgtgaaagaa gaagaatctt cttgtaaaat
    21181 agctaggccg tgtaatcttg cttttttcag agtagctgaa aaaagcaggg gcgtctcaat
    21241 gtgaatggtc aagaaggcta agcctggggc accttcttgg tgttttttga ggtaaggggc
    21301 tgaccagtga ttgaatgcaa ggtgatgatg gtagttccct gaagcaatcc agctagcact
    21361 tggaatggtc attttatcgc ctaaatcaaa aaccttttga tagagaagtg aagaagccag
    21421 agcatttttg acactcagat ggacatggcc aatgcgtgtg tcttgagcta gtaaaaagcg
    21481 cttggggata tctgtcaact gctccaatat gcttttggta tccgtgggct cagtcacacc
    21541 aataatttga ccattgtcgc ggatatccca atgctcaaca gctttgtcat gataaatttc
    21601 aatcccattg ccctcaggac cgctgaggta gatagcttcg ctatagccgt gatcagctga
    21661 tccttctaaa gagatacctc tggttaagaa atgattgaga acaagtccca agctatgacg
    21721 atcaggaact aaaaaggctg tgtgataaag accataagcc ttatttgctg gtaaaggtgt
    21781 ttgtcgtaac tctaagataa cggttttacc gtctgtagtc aattgacgag atgtcgtgtc
    21841 ttgtgagaga acctgtaaac tgataatact tgtgtaaaag gttgtcatct ttgctagatc
    21901 ggtaacattt agagaaaccg ttcctaaaga gatggttgag ttgtaagggg atgtcatcct
    21961 attacctcca aaaactaatt aaatctatca taatcaatca gtttcaaaaa gaaaagtagg
    22021 cacaaataag tggtataggc actaaaaagt aactcatttg ttaaccctat cttgtttctg
    22081 ttctctctaa tagtctataa tgggttataa gttacaaaaa gatactaaga ggagctgctg
    22141 cctatgctga aaaaaagacc ggcttgtccc gtagaaacgg ccttatcggt aatcgggaat
    22201 aaatagaaat tattaatatt aagagacttg ctaaagggac cttgcgtttt gggcagttga
    22261 agtcctcaat aggtttggtc agtcaaaagg tacttactgc acaattaagg gctatggaag
    22321 cagacggatt ggttcatcgg gaagtgtatg cagaagtgcc acctcgggtg gagtattcct
    22381 taacagaaac tgggttgagc ttagctcctg ttattgaagc gatgtcggat tggggccaaa
    22441 cttaccaaga aaaacatgcc gatttatttg aagaaggcta gaggaaagag ttgtccgagc
    22501 atactgagac ttagagaagc tgatagtttt taaaaaatga tattatcaaa ttctttacag
    22561 tttgataaaa ttactttttt tctataaaaa gacttgcgct catctcacga atatgataat
    22621 ataatatcaa gtctgtttga gaaaacagaa gctcaggtca agtaagtgca cacttacaat
    22681 aacacagttg ataatgggga aactcagagt caagaaaaag aaaagcaagc cttgcatttt
    22741 gc
//
LOCUS       DAUCLR010000026        20919 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.026, whole genome shotgun sequence.
ACCESSION   DAUCLR010000026 DAUCLR010000000
VERSION     DAUCLR010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20919
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.026"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            141..416
                     /locus_tag="VGT87_000812"
     CDS             141..416
                     /locus_tag="VGT87_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEQ3813686.1"
                     /translation="MRTEKDIENYLKKKTKGLCLKFTSPGTIGAPDRIVVMNTGIFFV
                     EVKAPGKKPRPSQVAMHKKIKEAGQHVWVVDSYESVDMALKEMENWV"
     gene            413..1735
                     /locus_tag="VGT87_000813"
     CDS             413..1735
                     /locus_tag="VGT87_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEQ3813687.1"
                     /translation="MRLHEYQEYAKTWIVEHPYCGLLLDMGLGKTLTTLSAIDEIQNI
                     FSEDHKILIVAPKKVAEETWPTEIGKWHFDFTYSKVLGSEGKRIEALETEADIYLINR
                     ENVTWLVEYYKTKWPFTFVVIDELSSFKSSKSKRFRALRKVRPKVQRLVGLTGTPAPN
                     SLIDLWPQIYLMDRGARLETSQTRFKDKYFVPDKRNGLIIYSWALRDGAEAEIYNKIE
                     DICVSMKAKDYLKLPPRTNNVVSVKLSNMKAYKQLEADLVLEFKNKEISAANSAVLAN
                     KLLQMANGAIYDDDKATVAIHDDKLDALENVVEESQGQPILVFYQYQHDLERLKKRFP
                     QAEELTSVDKWNSGKIPILLCHPQSAGHGLNLQKGGHIIVWFGLTWSLEYYQQANARL
                     DRQGQTEPVIVHHIVAENTVDEKVLRILQGKEKNQNALLEAVKAQLGV"
     gene            1736..1903
                     /locus_tag="VGT87_000814"
     CDS             1736..1903
                     /locus_tag="VGT87_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813688.1"
                     /translation="MKKEYVVRIYTGREKNFEAKPQFEEKTFTRKADMLKFWNSCEAT
                     VKEKYTREWKK"
     gene            1900..2166
                     /locus_tag="VGT87_000815"
     CDS             1900..2166
                     /locus_tag="VGT87_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813689.1"
                     /translation="MTEELIKPLLEEIERKDDRLYMQSNVIAVLMTAVLMLLMISIVL
                     QNHYEPQITGLRAQLSRTQKQLKRASEDRARQTKRIVELTGNGG"
     gene            2317..2508
                     /locus_tag="VGT87_000816"
     CDS             2317..2508
                     /locus_tag="VGT87_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813690.1"
                     /translation="MKIEFFLKQGPTKSVSNYGEICIDEVIGAMKNNDVIALNGKNNA
                     VIIPKQNISHVIIHKEVTG"
     gene            2505..2921
                     /locus_tag="VGT87_000817"
     CDS             2505..2921
                     /locus_tag="VGT87_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF722 domain-containing protein"
                     /protein_id="HEQ3813691.1"
                     /translation="MSRLSNAQLKAFDEWLFDYRFIDNKIALRKLELQTDMNTEVDKN
                     IGGGRANFVSKVTEDVVARWDSDRELKGLSNFKEAVIATLNLLDEELTDIFNLRWGVG
                     SSNTWEEIAYTRHLSRKSIYRKRERILEIFSKKIGM"
     gene            3011..3463
                     /locus_tag="VGT87_000818"
     CDS             3011..3463
                     /locus_tag="VGT87_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEQ3813692.1"
                     /translation="MLGGDGKLSKLTLKQKRFADEYIISANATAAAIKAGYSKKTARS
                     IGQENLTKPDIKAYIDERLEKLESEKIATQEEVLQYLTSIMRGDQQEKTLISVGEFGQ
                     KIVDIDVGAKDRIKAAELLGKRYRLFTDKVEMDVSSDVTINVGEWDDD"
     gene            3453..4730
                     /locus_tag="VGT87_000819"
     CDS             3453..4730
                     /locus_tag="VGT87_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEQ3813693.1"
                     /translation="MTTKQRPKINIVIKHPSKVFNKHIYDKLYNYSNFTEVHYGGASS
                     GKSHGVFQKIILKALNPKFKHPRKILVLRKVGATVRDSVFADIMSNLSYFGILDKCKI
                     NMSAFRITLPNGSEFIFKGMDNPEKIKSIKGISDVVMEEASEFTLDDYTQLTLRLRDK
                     KHLEKQIYLMFNPVSKVNWVYKAFFVKTPKNTVVYQTTYKDNRFLDDVTRENIEELAD
                     RNEAYYKIYALGQFATLDKLIFPKYDKQILNKDKLSHLSSFFGLDYGFINDPSAFLHV
                     KIDDANKKLYVIEEYVRKNLTNDKIANAIKDLGYTKEEIRGDSAEKKSNQELRNLGIP
                     RMIDVTKGPGTVMQGIQYLLQYDWVVDERCVKTIEELENYTWKKDKKTNEYTNEPVDS
                     YNHCIDAIRYAVQDRIYQSADRSKRMKNAKYYF"
     gene            4746..6279
                     /locus_tag="VGT87_000820"
                     /pseudo
     CDS             4746..6279
                     /locus_tag="VGT87_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560651.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            6239..7687
                     /locus_tag="VGT87_000821"
     CDS             6239..7687
                     /locus_tag="VGT87_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEQ3813694.1"
                     /translation="MLVVKKNQRQKSLNYWQKRQEDILSYLDRTDLDVFSELQKLYNE
                     QAFELQKELFDFYTKYSEENKMTYQDVVKKLRHEDLSDYVANANKYRKQAEKDPELLK
                     RLNEQYVSARATRMDALNLELVYRAGILKGVLDSAFENHLKKVASYAYKKAMGGRSGT
                     INGPVLEELVRTPFDGYNYSEQLWGNTDNLVKNLQKRLKQGFVRGEHPRAMARDLAKR
                     FNVANHRAETLIRTDGTMVINNATARRYLNAGLKYYRDLVRLDDRTTEICRTIAKENK
                     RKLLSELKPGINVAPYHFNCRTTIIPDEDELSIEVEPIDDKSTKYFKDVTSDWIDGNE
                     HKPQLSLLNEYVKNGTPYKVDGHSVVLDHSNYEYRVANWLSKKTGLQVDMVPRVNSPE
                     HIKTPDYLVDVAPFDLKEITGSGKNVIDGNLRKAKKQATNIIFDITKTPLSFEEIMGQ
                     LEHIYMIDRRGLDISIIKNKDEVLAVLEKEGG"
     gene            7715..7903
                     /locus_tag="VGT87_000822"
     CDS             7715..7903
                     /locus_tag="VGT87_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813695.1"
                     /translation="MGVRPSSFFISIIHHPIKTIKEENMFIWQMVLVALGVLVLILIV
                     GIAAIAVKSIIAELKKEG"
     gene            7908..8174
                     /locus_tag="VGT87_000823"
     CDS             7908..8174
                     /locus_tag="VGT87_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813696.1"
                     /translation="MNKRIKKKRKLETAVVMLVAENAMQAEAIKNQNKQIMELKSIVQ
                     RNALATNEELAAVKAATLDNQSVIKAIGDTVDYIKKNYKRKWGK"
     gene            8342..8911
                     /locus_tag="VGT87_000824"
     CDS             8342..8911
                     /locus_tag="VGT87_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEQ3813697.1"
                     /translation="MAEENVTTETTEQVDTQKEAVEYPKHEYERTFTRADISKMMAAE
                     RTKWESEQSAAIEKARTEGERLAKLSKDERAKEEEQKRLDAIAEREKAVAEREMRIET
                     HSLLVEKGLPLDFIDIVLATTAEEVKANIDNLQTIFDKAVEKRVNDRLTQKPPRTGNG
                     SVGMTKADIMAIEDDDERMRLIAENRNLF"
     gene            8924..9811
                     /locus_tag="VGT87_000825"
     CDS             8924..9811
                     /locus_tag="VGT87_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HEQ3813698.1"
                     /translation="MAEKNLNTMADLGDIKSIDFVNKFSKNINDLLKLLGVTRRETLT
                     NDLKIQTYKWEVTLDQTDPGEGETIPLSKVTRTKDKDYTVKWFKKRRATTAEAIARHG
                     AARAITEADKRIMRELQNGIKDAFFTFLKTKPTKVKGVGLQKALSASWAKLATFNEFE
                     GSPLVSFVSPLDVANYLGDTKVGADASNVFGMTLLKNFLGMQNVIVMPSVPEGKIYST
                     AVENLVFASLNVKGGDLGGLFADFTDETGLIAAARNRQLSNLTYESVFFGANVLFAEI
                     PEGVVEATIEAAAVPGIGG"
     gene            9823..10179
                     /locus_tag="VGT87_000826"
     CDS             9823..10179
                     /locus_tag="VGT87_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HEQ3813699.1"
                     /translation="MGDKQLIDDIKLFIGISKGDGAQDELITLAIYESKERVLAKLNE
                     YSETEITKIPDRLRFIVRDVAIKRFNRINSEGAVEDSEEGKTFKWDSYLKEYESTLRS
                     AAIGKVYSGKGVARFI"
     gene            10190..10468
                     /locus_tag="VGT87_000827"
     CDS             10190..10468
                     /locus_tag="VGT87_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813700.1"
                     /translation="MIYKDRVILVYVDEQDDFLDKRTVEKPSGKIPCMENTFTKSEQM
                     GLFGKYDLNAFKLHLQGHYDGFSKIIYKGKSRLIKGLAHHKNSTVIYV"
     gene            10465..10809
                     /locus_tag="VGT87_000828"
     CDS             10465..10809
                     /locus_tag="VGT87_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEQ3813701.1"
                     /translation="MSLIYRMRGLDRFLRSVERKQKSVRIAVDKELSKSAARIERQAK
                     ILAPVDTGWLRAQIYSEQQRLLHYRVVSPALYSIYLELGTRKMEAQSFLDPALRKEWP
                     VLMANIKKMFKR"
     gene            10813..11172
                     /locus_tag="VGT87_000829"
     CDS             10813..11172
                     /locus_tag="VGT87_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813702.1"
                     /translation="MDYSLETLYLKKVKNRLGVLDIPIYFKLPKSDVLEPFIVVGTNI
                     SDLSKTAQTGAVIDDFSLNIDAFLPGDSRLDAEEIKSRMLRLLGRNNQIKAQILVDNS
                     IGREVYRVAINITETLF"
     gene            11184..11783
                     /locus_tag="VGT87_000830"
     CDS             11184..11783
                     /locus_tag="VGT87_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HEQ3813703.1"
                     /translation="MANTKKGTTIEITTGKPIVGKKIFYFIQSVDAPKKSQALLPAYR
                     TDGTTTMGGEYIDEQTQQGRVIEKATDEHSIDLTTYFVPTDPSVAVIEEAKKTGKSIK
                     IWEVIADESVKEQIQIPESTGPKKDVYPAKFGYAKIDEIERGTGISDLVEMSYTANIV
                     GALQDGKFPLTKEEIEMLENVYGYQNPGDTTGDYDNITK"
     gene            11837..12292
                     /locus_tag="VGT87_000831"
     CDS             11837..12292
                     /locus_tag="VGT87_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813704.1"
                     /translation="MEFTAAKRNIDIKFDFKTMFKINNKLGTINPETGERNADGVGAL
                     FFNILERNESAIVDLVRLSAGSGKKALTEDEILDAIAESVDEEGTTEGLFAEIEKEMV
                     DSGFFRAKILKYIENMEKSARYLKAKDDMDATQIQIIEDMIGRMSNAVS"
     gene            12367..12600
                     /locus_tag="VGT87_000832"
     CDS             12367..12600
                     /locus_tag="VGT87_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813705.1"
                     /translation="MEGLEYKQLAERESLSELSLKLRYTLNAKKVDVGKLKYDKHRLT
                     IKRSYQKASRSQADSDSSIVERIQMLNNHFQNR"
     gene            12615..16997
                     /locus_tag="VGT87_000833"
     CDS             12615..16997
                     /locus_tag="VGT87_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEQ3813706.1"
                     /translation="MPGTFDGSIFADVGANTKDYEQAMARIVSTTQNAFRKAQDTAVN
                     SSNKMVQIIGQIMAQLANNGESLGKRLGSAYATGLKLSIGEIQRIAASIGEKIPEPIK
                     NGFQKIIIPVASVTDKIRGNLTGFINQTKMQLQNLVGIGKIRSAFAKAATGVDILTQR
                     ASSKLNNLSAIFETVAGKLPRPFGTAFRKIASSVSTLNSSIQSVGGKISNSLGQQVLN
                     PALQSWNNFFSSVSAKASAFANKVSTSLFGRLTSSLANLSSKIGSSLSNGFSRMSSSA
                     ATSLNGISQKFANTSSAGERLKSTVMSIVQAFSLMAVAQKAMHAITGAIDGAVSRVDT
                     MNRFPKTMALFGYSAEQSKASIDKLSKGIEGLPTPLDSAVKSAQQLAITTGSLDKGTS
                     LALAFNNAMIGYGATTEGAEQALRQFSQSLGSGKIQAEEFNSVSEAAPGLMSKMAEAF
                     GFGKNGVQDLKSALSDGKITAQEFADKMIELNDAQGGFAEMAQSSAGGIRTAWKNVHT
                     AVVKGVAGMISAFDEAAKANGMKTIAETLLSLKPAITSVFDTINSLIPNAVAAFARLK
                     QSINIDFSPLGASVKEVFALINTVLRDFAHTGELSGQAFDNLKAKITSLAPKVIALWA
                     VMNPASAISTIMPLLSLFGKVALALGSLGTSVGAFGGIISSGIASASGVVGAFAATLS
                     GLPGVFATAAGRGLSVLGTMTSAMSSLVSLALAAIGPAAILGLVVAGLGLINSQFGAQ
                     IEQLLNTAVTKGPGIIQGLVKGITSKIPVLIASGTQLIAKFANAITVLLPVIIQAGVQ
                     LITSLVQGIGQNATNLISSAIKIIGSFVSSIASALPQLISVGMELLLNVVNGIVQNIP
                     LIIQQAQQIIDSFGNSLQANLPSIISNGIAILVNLVQGITQMLPTVLQIATQVITSFV
                     SGIVQFLPQLLQGGIQIIISLVQGIIQNLPQIAQSAVQIIQFLASGLTQALPQIIAAG
                     IQLVAQLAVALIKGLPQIISAGIQLIMGLGKAMLGAIPNALSGVWEGIKSGFSSMWDQ
                     ITGKSSTSTAKVSADATVMAMNVGMQTTAIANQANTDTTSMLNSISQNTELANINATS
                     NATQMANNVNAQTGTMSLRGLNDSMSLASGISANMSVANINATTQAREMASGVNGATS
                     SMNLDAINHTLSLASGVGANMGAASTGATSQAQAMSSGVSNSLASMQSNSTKAASGLS
                     NSVTSEMSSAATSATSSVNRLSSAVESGFNKAKTSATTSMNGIANAVKTGFSSINSTA
                     KQSMANLVSSVTSGMSRATAVSNNACQKILSIFRALAGQMSSAGAYAGQGFANGLASS
                     AGTIYAIANSIAANVAATIRRALDIHSPSRVTKTLGAFTGEGFALGMAEWIGEINSLG
                     KAYATAVTDQNWGVNSTVSTSAKVNNSGINTSLDNLSEEVRQSQLSEPIFEVHNEIVG
                     DKIYTAVKEKESREQSKDSYFVFD"
     gene            17009..17851
                     /locus_tag="VGT87_000834"
     CDS             17009..17851
                     /locus_tag="VGT87_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEQ3813707.1"
                     /translation="MDLLIEKESQATRLSDFGIYNIAIEDSAPLLSVSHRAVKGRSGY
                     IYDGATFTTKTLKVKGRVTISNVERLLDKQDELNALLVVDEPFYVTKMYPENSDLFNF
                     ELPGESTGDLQLIGSPHKPWKYRFKVILDDTIAYEFIGKTSQGLKYNLSFALRTAELP
                     FGETKPKDITLSGGSFAYAGTAKASQLEWPFIIELTPSGGQTNFYIEIDGRRFEFKQN
                     SQLQNSDKLLLTGIATTLNGNYVNAKTNYEYFIFNPNPNKRITYKTDFLGTIRILNFV
                     ELYK"
     gene            17861..19840
                     /locus_tag="VGT87_000835"
     CDS             17861..19840
                     /locus_tag="VGT87_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEQ3813708.1"
                     /translation="MITFLDHRDIEYGVISVIRHTNAVNGERSVSGEIYTNSDVLNNI
                     DRGWRLRFEDEYYVVIYAKPVDVGQKTQVSFDAIHQFFWDFSKSSIYEGLGDGSHTID
                     TYLETVFKGSGYRYKLEVGVNAFRKQSFNYKSRLDLFNEIIKATGLEFSVSGKVVRLL
                     KNIGTDLSTVVRKNFNMNDLTIEKNIDSFITYQKGFGAWTDPEDHSKGRLEAEYESPL
                     AKEYGRLEGAPLTDERFTVADNLKEALKSNVENSYKISVKIDMEDLTRAGYRCERPVA
                     GDYIMAINETLGFQERIRIVSFTSYYDATGALVKHEVTCNDIGSVKKQSVGSLSINSR
                     INQIDADIASAIEVATQALVSADGKNTVYGGTEMPKDEPKGTLKKGDILFLKVGDTTR
                     MYFWNGAEWEEPEVVNDPERWREDLEKQISEAIEKAKKAQEEINQRTDKELEEFRAAL
                     KNLALPEEAIKKITEAIKVDDIPSIKQSFDDLKNRVSETSEESRLTAEILGNNGKTRY
                     NKNLLVGDPNRVKTYDQDYIEVEANDGGFKRGETYTISFSQTCELLKEVAITLTQINN
                     KGVKLVLTPTKAKMDAQTFEVTKDKQSIEVYPLSYTGVLTGDWYKPKQIDLNASDTQE
                     LALEMDYKDVVDGKDATITGAWSDSPQIILYGGKK"
     gene            19837..>20919
                     /locus_tag="VGT87_000836"
     CDS             19837..>20919
                     /locus_tag="VGT87_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronoglucosaminidase"
                     /protein_id="HEQ3813709.1"
                     /translation="MTETIPLRVQFKRMKAAEWASSDVVLLEGEIGFETDTGFAKFGD
                     GQNTFSKLKYLTGPKGPKGDTGLQGKTGGTGPRGPAGKPGTTDYDQLQNKPDLGAFAQ
                     KEETNSKITKLESSKADKNAVYLKAESKIELDKKLNLKGGVMTGQLQFKPNKSGIKPS
                     SSVGGAINIDMSKSEGAGVVVYSNNDTSDGPLMSLRTGKETFNQSALFVDYSGKTNAV
                     NIAMRQPTTPNFSSALNITSGNENGSAMQIRGVEKALGTLKITHENPSVEANYDENAA
                     ALSIDIVKKQKGGKGTAAQGIYINSTSGTTGKLLRIRNLGDDKFYVKSDGGFYAKKTS
                     QIDGNLKLKDPTANDHAATKAYVDKAI"
BASE COUNT         7007 a         3611 c         4641 g         5660 t
ORIGIN      
        1 tttttcttct attaacttaa atataaatat aaaaaataaa tatatataaa ataaggtata
       61 tataaaacac gtatataaaa aagaattaat aagttgaaaa aaaaacgtaa ccctgacctt
      121 tattgaccaa agggggaagg atgaggactg aaaaagatat tgaaaattat ttgaaaaaga
      181 aaacaaaagg gctgtgttta aaattcacca gtccagggac gataggagcg cctgacagaa
      241 ttgttgtcat gaatacggga atcttttttg tagaggtcaa agcgcctggt aaaaaaccaa
      301 gacccagcca agttgccatg cacaaaaaaa taaaagaggc tgggcagcac gtttgggttg
      361 ttgactccta cgaatcagtg gacatggcct taaaagaaat ggagaactgg gtgtgagact
      421 gcacgagtac caagaatacg ctaagacatg gatagtagag cacccttatt gtggcctttt
      481 acttgacatg ggccttggta aaacgctgac aacactatcg gcgatagatg agattcaaaa
      541 tattttttcc gaggatcata agattttaat cgtagctcct aaaaaagtgg cggaagaaac
      601 gtggccaacg gagattggga aatggcattt tgatttcacc tactctaaag ttttggggag
      661 tgagggaaaa cgaattgaag ccttagaaac agaagccgat atctatttga ttaatcgtga
      721 gaatgttact tggcttgttg aatactacaa gactaaatgg ccgtttacct ttgtggttat
      781 tgatgagctg tcaagcttta agtctagtaa gtcaaaacgg tttagggctt tgcgaaaagt
      841 tagaccgaaa gtccaacgtc ttgtaggact aacaggaacc ccagcgccta acagtttgat
      901 tgatttgtgg ccgcagattt atctgatgga cagaggcgcc agacttgaga cgagccagac
      961 tcgatttaaa gacaagtatt ttgttcctga taagcgtaat ggcctaatca tttacagttg
     1021 ggcacttagg gatggtgcag aagcagaaat ctataacaag attgaggaca tctgtgtcag
     1081 catgaaagct aaagactatc tcaagttacc gccgcgaacc aacaacgttg tatcagttaa
     1141 gttatctaat atgaaagcct acaaacagct tgaagctgat ttggtgttgg agtttaaaaa
     1201 taaagaaata tctgcggcta attctgcggt tttggccaat aaattacttc aaatggccaa
     1261 tggtgctatc tatgatgatg ataaagcaac agttgctata cacgacgaca aacttgacgc
     1321 gcttgagaat gtcgttgaag aaagccaagg tcagccaatc ttagtttttt accagtatca
     1381 acatgatctt gaaagactta agaaacgatt tcctcaggct gaagagctga cgtcggttga
     1441 caagtggaat tccggaaaaa taccaattct tctgtgtcac ccacagtcag ccggacatgg
     1501 gcttaatctt caaaaaggcg ggcatattat tgtttggttt gggctaacat ggagtcttga
     1561 atattatcaa caagctaatg ccagattaga tagacaaggg cagacagaac ccgttattgt
     1621 acaccacatt gttgcagaaa atacagttga tgaaaaagta cttaggattt tacaaggcaa
     1681 agaaaaaaat cagaacgcct tactcgaagc agttaaggcg cagttaggag tctagatgaa
     1741 aaaagaatat gtcgttagaa tctacacggg tagagaaaag aattttgagg caaaacctca
     1801 gtttgaagag aaaaccttta cgcgaaaagc ggacatgttg aagttttgga attcttgcga
     1861 agcaacagtt aaagaaaaat atactaggga gtggaaaaaa tgacagaaga actaataaaa
     1921 ccgctgctag aagagataga gcgaaaagat gatagattgt atatgcagtc taatgtaatt
     1981 gccgtcttaa tgacagcggt tcttatgtta ctgatgataa gtatagtctt acaaaaccac
     2041 tacgaaccac aaataacagg actgcgtgct cagctaagta ggacacaaaa gcagcttaaa
     2101 cgtgctagtg aggatagggc cagacagaca aaacggatag tggaattgac gggtaatggg
     2161 gggtaggtat gaatataatc catagaatgg aaaacgggat agatgctgta ggtgatttaa
     2221 caacttatgc agtaccatgt tgtccagagt gtaaaaatga aacatatagt cagaaacact
     2281 gtcctttttg cggtgaaaat cttatggagg tagcaaatga aaattgaatt ttttttaaag
     2341 caaggtccta ctaagagcgt ttctaattat ggtgaaattt gtattgatga agtaattggc
     2401 gcaatgaaaa ataacgatgt cattgctcta aatggaaaaa acaacgcagt tattataccc
     2461 aaacaaaata tttctcacgt aattattcat aaagaggtaa cgggatgagt agattatcta
     2521 atgcacagct aaaggctttt gatgaatggt tgtttgatta tcgttttatt gataacaaga
     2581 ttgcacttcg aaaacttgag ctacaaactg atatgaatac agaagtagat aaaaatatcg
     2641 gtggaggaag agcaaatttt gtttcaaaag ttactgagga tgtggttgca aggtgggatt
     2701 cagataggga actaaagggt ctatccaatt tcaaagaagc agttattgca acgctaaatc
     2761 ttttggatga agagctaact gatattttta atcttaggtg gggtgttggt tcaagtaaca
     2821 cttgggaaga aattgcctat acaagacatc tgtcacgtaa gtctatttac cgcaaaagag
     2881 agcggattct agaaatattc tccaaaaaaa taggaatgtg acacaaaaat gtatggaatt
     2941 gtcatctttt tgatgataaa ttgatagtat gagtttaaag gtaaggcaaa tagtgtcttg
     3001 cctttttgtt atgcttggag gtgatggaaa attgagtaaa ttaaccctaa aacagaagcg
     3061 ttttgcagat gagtacatca tctcagctaa cgcgacagca gctgctatta aagcagggta
     3121 tagtaaaaag acagcaaggt caataggtca agaaaacttg accaaacctg acattaaagc
     3181 ttatatagac gagcggcttg aaaaacttga atctgaaaag attgctacgc aagaagaagt
     3241 cctacaatat ctcacttcaa ttatgcgagg agaccaacaa gaaaagacgc tcattagcgt
     3301 tggagagttt ggccagaaga tagttgacat cgatgttggt gccaaggata gaatcaaggc
     3361 agctgagcta ctaggcaaac ggtacaggtt atttactgac aaggttgaaa tggatgtcag
     3421 ctcagatgta accattaacg taggtgagtg ggatgacgac taaacagcgc cctaaaatta
     3481 atatcgtaat caagcatcct agcaaagttt ttaacaagca tatctacgac aaactttaca
     3541 actatagcaa cttcacggaa gttcattatg gtggggcgtc tagcggaaag tctcacggtg
     3601 tttttcaaaa gataatttta aaagcactta accccaaatt caaacatccc agaaagatat
     3661 tagttctcag aaaagtcggt gcaaccgtta gagactctgt atttgctgat atcatgtcta
     3721 atttgtcgta tttcggcatc ttggataaat gtaagataaa tatgtcggct tttagaataa
     3781 cgctcccgaa tggctcggaa ttcattttta agggtatgga taaccctgag aagattaagt
     3841 caattaaggg gatatctgat gtcgtcatgg aagaagctag tgagtttacg cttgatgatt
     3901 atacgcagtt gactttgcgt ttaagagata agaaacatct agagaagcaa atatacttga
     3961 tgtttaaccc tgtgtcaaaa gtaaactggg tttataaagc cttcttcgtt aagacaccta
     4021 agaatacagt tgtctatcaa acaacttata aagacaaccg cttcttagat gatgtcacga
     4081 gagaaaatat tgaggaacta gccgatagga atgaagctta ctacaaaata tatgctcttg
     4141 gccagtttgc tacacttgat aaactaattt ttcccaaata tgacaagcaa atattaaaca
     4201 aagacaagtt atcacacttg tcttcttttt ttggtttgga ctatgggttt atcaatgacc
     4261 cttcggcatt tttgcatgtt aaaatcgatg acgcaaacaa gaagttatac gtcatagagg
     4321 aatatgtcag aaagaatttg accaatgaca aaatagcaaa tgctataaag gacctcggct
     4381 ataccaaaga agaaatcaga ggagattcgg ctgaaaagaa atctaatcaa gagctgagga
     4441 atttaggtat tcctagaatg attgatgtta ccaaagggcc tggaaccgtt atgcaaggaa
     4501 tacagtattt gcttcaatat gattgggttg ttgatgaaag gtgtgtcaag actattgaag
     4561 aactcgaaaa ttacacttgg aagaaagaca agaagactaa tgagtatacc aacgaaccag
     4621 ttgacagcta taaccactgc attgatgcca taagatatgc cgtacaagac agaatatacc
     4681 agtcggcgga tagaagtaag cgcatgaaaa atgctaaata ttatttttag gaatgaggtg
     4741 aaactttgga agaaaaacaa tttttagcag gaactcgttt taatgagaat gctaataagc
     4801 aatttattat gctgcaagaa gactttgaag caattgacta tgaatcaaaa ctttggatag
     4861 atcaacttaa gaattacatt tcaaggttta aagcagaaca attagagcgt ttgaaagagt
     4921 taaagcgata ctatcttggg gataataaca tcaagtatag acctgctaaa acagataaat
     4981 acgcagcgga taatcgtatt gcaagtgatt ttgctaagta tattacggta tttgagcaag
     5041 gttatatgct tggcgttccc gttgagtaca aaaatgaaaa taaagacctc caggcagcta
     5101 ttgaccttat gtcggtcaga aacaatgagg actaccacaa cgtcaaaata aaaacagatt
     5161 tatcaattta tggaagagtc tacgagctgt taactgttga gaaaatagat gacaaaaaaa
     5221 accgaagtaa aactctacca gctcccggcc gagcaaactt ttgttatcta tgatgataca
     5281 taccagcgaa attcgttgat ggccgttcat ttctacgaca tagattacgg ctcaggtaaa
     5341 cgtaaacaga ttatcaaagc atatacttcc gacacaattt atacctacga agactataac
     5401 cttgaaacaa aaggtatgcg attaaaagat tatgaaggac atttttttaa aggtgtacca
     5461 gtcaacgaat acgctaataa cgaggagcga acgggggctt atgagtctgt acttgataat
     5521 attgacgcct atgatttatc tcaatcagag cttgctaact ttcaacaaga ttcagtcaat
     5581 gcgttgcttg taatagctgg taatgcttac acaggagctg atgagaatga ctacttagat
     5641 gatggccgat taaatcctaa tggtcgttta gcgatctcaa ttgggtttaa aaaagcccaa
     5701 gtgttaattt tagacgataa tcctaatcca aatggcgtta aaccgcaagc gtactttctc
     5761 aaaaaagagt atgataccgc cggtagcgaa gcctacaaaa atagattagt tgcagacatt
     5821 ttgaggttta cttttacgcc agatacacaa gatatgaaat tttcaggagt tcaatctggt
     5881 gaatcaatga agtataagct tatggcttct gacaactatc gcgagaagca agagaggttg
     5941 tttaaaaaag gattaatgcg acgcttacgc ttagcagcta atatctgggc catcaaaggg
     6001 aatgaagcaa ctacatatag ccttgtaaat gatacgagta tagttttcac acctaatctt
     6061 cctcaaaacg ataatgaaat tgttacagct gcacaaaatc tttatggcat tgttagcgat
     6121 cagactatct ttgaaatctt aaatactgtc acaggagtag atgctgaagc agagcttaaa
     6181 cgcttgaaag aagaagctga taaaaagcaa tctttaccgg agcctagatt ggtaggtgat
     6241 gctagtggtc aagaagaacc aacggcagaa aagccttaat tactggcaaa agcgtcaaga
     6301 agatattcta agttatttag acagaactga tttagatgtc ttttcagaac tgcagaagtt
     6361 atataatgaa caggcttttg agttacaaaa ggaattgttt gatttttata ctaagtattc
     6421 tgaagagaac aaaatgactt atcaggatgt cgttaaaaaa ctacgtcatg aagatttatc
     6481 agattacgta gctaatgcta acaagtatcg taaacaagct gagaaagatc ctgagctgct
     6541 aaaacgactt aatgagcagt atgtatcagc aagagctaca aggatggatg cattaaatct
     6601 tgagcttgtt tatcgtgcgg ggatactaaa aggtgtactt gattcagcat ttgaaaatca
     6661 tttaaaaaaa gttgcctcat atgcttataa aaaagcaatg ggtggacggt cagggacaat
     6721 caatggtcca gttttagaag aactggttag aaccccgttt gatggttata actattcaga
     6781 acagttgtgg ggcaatactg acaatcttgt taagaatctt caaaagagat taaaacaagg
     6841 ttttgttcgt ggcgagcatc ctagagcgat ggccagagat ttagctaagc gatttaatgt
     6901 tgctaatcac agggctgaaa cactaatcag aactgatgga actatggtga ttaataatgc
     6961 taccgctagg cgctacttga atgcaggact taagtattat cgtgatttag ttagacttga
     7021 tgacaggaca actgagatat gccgtacaat tgctaaagaa aacaaaagaa agctattatc
     7081 tgagctaaag cctggaatta atgtagcacc ctatcatttc aactgcagaa caactattat
     7141 tcctgatgaa gatgagctaa gtattgaagt agaaccaatt gatgataaaa gcactaagta
     7201 ttttaaggat gtcacctcag attggataga tggaaatgag cataaaccac aactgtcact
     7261 actaaatgag tacgtaaaaa atggcactcc ttacaaagta gatggccata gtgtcgttct
     7321 tgatcattcg aattatgaat atagagttgc taattggtta tccaaaaaga caggattgca
     7381 agttgacatg gtgccaagag ttaattcccc tgaacatatc aagactcctg attatttagt
     7441 tgatgtagct ccttttgatc taaaagaaat cacaggttcg ggtaaaaacg tcattgatgg
     7501 caatctacga aaagcaaaaa agcaggcaac taatatcatt tttgatataa caaaaacccc
     7561 tttatcattt gaagagataa tggggcaatt agaacatatt tatatgatag atcgtagggg
     7621 tcttgatata tcaattatca aaaataaaga tgaggtatta gctgtattag aaaaagaagg
     7681 aggatgaccc accgcctcca cagtaaactg cttcatgggc gttagaccat catccttctt
     7741 tatctcaatt atacatcacc ctattaaaac tatcaaggag gaaaatatgt ttatttggca
     7801 aatggtatta gtggcactag gtgtattggt actcatattg attgttggca ttgcagcaat
     7861 agcggttaag tcaattattg cagaattgaa aaaagaagga taaaacaatg aataagcgca
     7921 ttaagaaaaa acgtaaattg gaaacagcgg ttgtgatgct tgttgcagag aatgccatgc
     7981 aagcagaagc tattaaaaat caaaacaaac aaatcatgga gctaaaatca atcgttcaac
     8041 gaaacgctct ggcaacaaac gaagagttag cggctgttaa agctgctact ttagataacc
     8101 aatcagttat caaggcaatt ggtgacacgg ttgactatat taagaaaaac tacaaacgga
     8161 agtgggggaa ataaagttta accgtgtcga atttgacctc tttacaaatc taaaccaaag
     8221 tcgtagcaat acggcttttt attgtgtcca aactttgttg atgacactaa aagctacact
     8281 gtttcgtcgc aggacgtaaa gctagactat cggttggtgg cgtaaccact aggagaaagt
     8341 tatggcagaa gaaaatgtaa caacagagac aactgagcaa gtcgacactc aaaaagaagc
     8401 tgttgaatat cctaagcatg aatatgagcg aacttttact cgcgcagaca tctcaaaaat
     8461 gatggctgct gaacgcacta aatgggaatc tgaacaatcc gcagctattg aaaaagctcg
     8521 tacagaaggt gaacgtttgg ccaagttgtc aaaagatgag cgcgctaaag aggaggaaca
     8581 aaaacgtcta gatgctatcg cagagcgtga aaaagcagta gcagagcgtg agatgcgcat
     8641 tgagacgcat tccctgcttg tggagaaagg attgccattg gattttattg atattgtttt
     8701 agccactact gcagaagagg ttaaggccaa tattgataat ttacaaacta tttttgataa
     8761 agctgttgaa aaacgtgtta acgatcgttt aactcagaaa ccgccacgaa ctggaaatgg
     8821 ctcggtcggt atgactaagg ctgacatcat ggcgatagag gatgacgacg aacgtatgcg
     8881 tttaattgct gagaatcgta acttatttta agaggggaat attatggctg aaaaaaactt
     8941 aaacactatg gcggacttag gagatattaa atcaattgat tttgttaaca agttttccaa
     9001 aaatattaat gatttattaa aattgctagg ggttactcgt cgtgaaacat taactaacga
     9061 tctaaaaatt cagacctata agtgggaagt gactttagat caaactgatc ctggagaagg
     9121 ggaaacaatc cctctgtcta aggttactcg aactaaagat aaagactata cagtgaagtg
     9181 gttcaagaaa cgtcgtgcaa ctacagctga ggcaattgct cgccatggtg cagctcgtgc
     9241 tattactgaa gcagataagc gcattatgcg tgagcttcag aatggaatta aggatgcatt
     9301 ctttacattc ctcaaaacaa aaccaacaaa agttaaaggc gttggccttc aaaaagcgct
     9361 gtctgcatca tgggctaagc tagctacttt taatgagttt gagggttccc cgcttgtttc
     9421 ttttgtctct cctttagacg tagctaacta tcttggagat actaaagtag gtgcggatgc
     9481 ctctaatgtt tttggaatga cattgcttaa gaactttttg ggtatgcaaa atgtgattgt
     9541 tatgccatct gtgccagagg gtaaaatcta ctcaacggct gtagaaaatc tagtttttgc
     9601 atctttaaat gttaaagggg gagacttggg cggcttgttt gctgatttta ctgatgagac
     9661 aggtttaatt gctgcagctc gtaatcgtca gctttctaat cttacctatg aatctgtttt
     9721 ctttggggcg aatgtactgt ttgctgaaat tcctgaaggg gttgtagagg caacaatcga
     9781 agctgctgct gtacccggca ttggtggtta aaggctattt atatgggtga taaacaactt
     9841 attgacgata tcaaactctt tataggtatt tccaagggtg atggtgcgca agatgagctc
     9901 atcacccttg ctatatatga aagtaaagag cgtgtgctag ctaaacttaa tgaatactca
     9961 gaaactgaaa tcaccaaaat tcctgataga ttgaggttta ttgtccgtga tgttgctatt
    10021 aaacggttta ataggattaa ttcagaagga gccgttgaag atagcgaaga aggaaagact
    10081 tttaagtggg acagttacct taaagagtat gaatcaacac tcagaagcgc tgctattggg
    10141 aaggtatatt caggcaaagg ggtagcaaga tttatttagg agatacacaa tgatctataa
    10201 agatagagta atcttagtgt atgtcgatga acaggacgat tttctagata aaagaactgt
    10261 tgaaaaacct agcgggaaaa tcccctgtat ggaaaatact ttcacaaaat ctgaacagat
    10321 ggggctattt ggtaaatatg atttgaatgc ttttaagttg cacttgcaag gccattacga
    10381 cggctttagc aagattatct acaagggaaa atcgaggttg ataaagggac tagcacacca
    10441 taaaaatagc acggttattt atgtatgagt cttatttatc ggatgagagg cctagatagg
    10501 tttttacgca gcgttgagcg caagcagaag tcagtacgaa tcgctgtaga taaagagctt
    10561 agtaaatcag ctgctaggat tgagagacag gctaaaatac tagccccggt tgacactgga
    10621 tggctgagag ctcaaatcta cagtgagcaa caacgactct tacactatag agtggtttct
    10681 cctgctttat attctattta tcttgaatta ggtactcgta aaatggaagc acaatcgttt
    10741 ttagaccctg ccctgagaaa agaatggcct gtgctaatgg ctaatatcaa aaaaatgttt
    10801 aagaggtgat gcatggatta ctcactggaa acactatatt taaaaaaggt aaaaaataga
    10861 ttgggagttt tagacatacc aatctatttt aaattgccta aatcagacgt tttagagcct
    10921 tttattgttg taggtacaaa tatatcagac ttgtcaaaaa cagctcaaac tggagcagtt
    10981 attgatgatt ttagcctgaa tatcgatgct ttcttacctg gcgatagtcg tttggatgca
    11041 gaagagataa aatctcgcat gcttagactg cttgggcgaa ataaccaaat aaaggctcag
    11101 attttggtag ataattcaat aggacgggaa gtctatagag ttgctatcaa cattacagaa
    11161 acactatttt aaaggggacc taaatggcaa acacaaaaaa aggaacaact attgaaatta
    11221 caacaggtaa accgattgta ggaaaaaaga ttttttactt tatacaatca gttgatgcac
    11281 caaagaaaag tcaggctctg ttgccagcct atcgtaccga tggtactaca accatgggtg
    11341 gtgaatacat tgatgagcaa acccaacaag gtcgcgttat tgagaaggct acagatgaac
    11401 actctattga tttgacaacg tactttgtac cgacagatcc atctgttgct gtcatcgagg
    11461 aagctaagaa aacaggtaaa tctatcaaaa tttgggaagt tattgccgac gaaagtgtca
    11521 aagaacagat tcaaattcct gaatcgactg gacccaagaa agatgtttac cctgctaagt
    11581 ttgggtacgc caagattgac gaaatcgaac gcggtactgg tatttcagac ttagtagaaa
    11641 tgtcctacac cgctaacatt gtaggggcct tacaagatgg taaattccct ctcacgaaag
    11701 aagaaattga aatgctagaa aatgtatacg gctatcaaaa tccgggtgat actactggcg
    11761 actacgataa tatcactaaa tagcacacag gggtggcaac tgatacccca ttttgattta
    11821 aaaaggagat aaaataatgg aatttacagc agcaaaacga aatatcgata ttaaatttga
    11881 ttttaaaact atgtttaaga tcaataataa actaggaaca attaacccag aaacaggaga
    11941 gcgcaatgca gatggtgttg gtgctttgtt tttcaatatc ttagagcgta atgaaagcgc
    12001 cattgttgac cttgtgcgtt tatctgcggg aagcggaaaa aaagcgctaa ctgaagatga
    12061 aattctagat gcaattgcag aatctgttga tgaagaagga acaacagaag ggttgtttgc
    12121 tgagattgaa aaagaaatgg ttgattctgg ttttttcaga gcgaagattt tgaaatatat
    12181 cgaaaacatg gagaaatcag ctcgttatct caaagcgaag gacgatatgg acgcaactca
    12241 aatccaaatt attgaagaca tgattggaag aatgagcaac gcagtatctt agtaaattgc
    12301 gcacggctag ggctcacaga cattgatact atctataaat gtactaagtg ggagctagag
    12361 gctattatgg aggggcttga gtataagcag cttgctgagc gcgagagcct ctctgaattg
    12421 tctctaaagc ttagatatac attaaatgct aaaaaggttg atgtaggtaa gctcaaatac
    12481 gataagcata ggctaactat caaaagatcg tatcaaaaag ctagccgtag ccaagccgat
    12541 agtgatagca gtattgttga gagaatacaa atgcttaata atcattttca aaatagatag
    12601 ataaggagga gtagatgcca ggaacatttg atggttctat tttcgctgat gttggtgcca
    12661 acacaaagga ctatgagcaa gccatggctc gtattgttag tacgactcag aatgctttca
    12721 gaaaagccca agatacagcg gttaacagta gtaataaaat ggttcaaatc attggacaaa
    12781 tcatggccca actggcgaac aacggcgaat cgctcgggaa gagacttggc tctgcctacg
    12841 ctactggttt aaaactgagc atcggtgaaa ttcaacgcat agcagcttcc attggcgaaa
    12901 aaattcccga acccataaag aatggttttc aaaaaatcat tattccagtt gcaagtgtta
    12961 ctgacaaaat acggggtaac ttaaccggtt tcattaatca aacaaaaatg caacttcaga
    13021 atcttgtggg tattggcaag ataagatctg catttgccaa ggctgcgact ggagttgata
    13081 tcttaactca gagggcaagt agtaagctta ataacttatc tgcaatattc gaaacggtcg
    13141 caggcaagct tccgcgacca ttcggaacag cgtttagaaa aattgcctca tccgtttcta
    13201 ccctgaatag ctctatacag tctgttggag ggaagatttc taattcactt ggtcagcaag
    13261 tcttgaaccc agccttacag tcatggaata atttttttag ttctgtatca gctaaggcgt
    13321 ctgctttcgc aaacaaagtg agtactagtt tgtttggtcg gctgacctca agtttagcta
    13381 atttatcaag taaaataggg agcagtctta gcaatggttt tagcagaatg tctagttctg
    13441 cggctacttc cttgaatgga ataagccaga aattcgccaa tacctcttct gctggagaga
    13501 gactcaaaag tacagtgatg agcatcgtgc aggcctttag tttaatggct gttgctcaaa
    13561 aagctatgca cgctattact ggggcaatag acggggcggt tagtcgcgtt gataccatga
    13621 accgctttcc gaaaacgatg gcgctatttg ggtattctgc tgaacaatct aaggcatcaa
    13681 ttgataagct atcaaaagga attgaaggtc tgccaactcc tctagacagt gctgtaaaaa
    13741 gcgctcaaca gctcgccata accacaggaa gtttagataa agggactagt ttagccctcg
    13801 ccttcaataa cgcaatgatc ggttacgggg caacaactga aggggctgag caagcactca
    13861 ggcagtttag ccagtcgttg gggtctggaa aaattcaagc tgaagagttt aactctgtat
    13921 cagaagctgc gccaggttta atgtctaaaa tggcggaagc cttcggtttt ggtaaaaatg
    13981 gcgtacaaga tttaaaatca gccttatctg atggcaaaat cactgcacaa gagttcgcag
    14041 ataaaatgat tgagcttaac gatgcccaag gcggatttgc agagatggcc caatcatcgg
    14101 ctggtggaat ccgaactgca tggaagaacg tacataccgc cgtcgtaaaa ggcgtagcag
    14161 gtatgatttc agcctttgat gaagcggcta aagctaatgg catgaagacc attgctgaaa
    14221 cacttcttag tctgaagcca gcaataacca gtgtttttga tacgattaat tctcttattc
    14281 caaatgcagt tgcagcattc gctaggctaa aacagtctat caatattgac tttagcccat
    14341 taggcgccag cgttaaagag gtgtttgctc ttattaatac tgttcttaga gattttgcac
    14401 atactggtga attatcaggg caggcttttg ataatctaaa agcaaaaatc acttctttag
    14461 cccctaaagt cattgccctt tgggcagtga tgaaccccgc tagcgctata tcaacgataa
    14521 tgcctttgct ttctctattc ggaaaagttg ctctagcttt aggaagctta gggacttctg
    14581 tcggggcgtt tggcggcata atttctagtg ggatagctag tgccagcggc gttgttggtg
    14641 cctttgcggc aactctaagc ggattacctg gcgtttttgc taccgcagca gggcgtgggc
    14701 tatctgtgct tggaactatg acaagcgcaa tgtccagtct tgtgagttta gcactggcgg
    14761 ccataggacc ggccgctatt ctaggtcttg tggtggcagg acttggtttg attaatagtc
    14821 agtttggcgc acaaatcgag cagctactta acaccgcagt tacaaaaggt cctggtatta
    14881 ttcaaggcct tgtaaaagga attacttcta aaataccagt tttgatagct agtggcactc
    14941 aattgattgc taaatttgca aatgctatca cagtgttatt accagttata attcaagctg
    15001 gcgttcagct aattacgagt ctcgtccaag gtattggaca aaatgcaacg aatttaatta
    15061 gttcagcaat aaaaatcatt ggtagttttg ttagttcaat tgcgagtgca ttgccgcagc
    15121 ttatttctgt tggaatggag ctattattaa atgttgtcaa cggcatagtt caaaatatac
    15181 ctcttattat ccagcaagcc cagcaaatta ttgatagttt tgggaatagt ttacaggcta
    15241 accttcctag tattattagc aacggtatag ctattttggt gaatcttgta cagggaatta
    15301 ctcaaatgtt gccaactgtt ctgcagatag ctactcaagt cattacgagt tttgtatctg
    15361 gaattgtaca gtttttaccg cagttacttc aaggtggcat tcaaatcatt attagccttg
    15421 tgcaggggat tattcaaaat ctaccccaga ttgcacaatc tgctgtacag attatacagt
    15481 ttttagcttc tggactgacg caagctctac ctcagattat cgcagcaggc attcagcttg
    15541 ttgcgcaact agccgtagcc ttgataaagg gacttccaca gattatttct gcgggtattc
    15601 aattaattat ggggctcggt aaagccatgt taggggctat cccaaacgct ctttcaggag
    15661 tttgggaagg tattaagagc ggattcagtt cgatgtggga ccaaatcaca ggtaagagtt
    15721 ccacaagtac agctaaagtc tctgcggacg ccacggtcat ggccatgaat gttgggatgc
    15781 aaacgacagc tatagctaat caagctaata cagatacgac atctatgctt aatagcatta
    15841 gtcaaaatac agagcttgct aatataaacg ctacatcaaa tgccacacag atggctaata
    15901 acgttaatgc tcagacagga acgatgagtc ttcgggggtt gaatgattcg atgtctcttg
    15961 cgagtggcat tagcgctaat atgtctgtcg ctaatataaa cgctactact caggctcgag
    16021 aaatggcttc aggggttaat ggtgcgactt cttcaatgaa tctagacgcc attaatcata
    16081 cgctcagctt agctagtggt gttggtgcga acatgggagc tgctagcaca ggtgcaactt
    16141 ctcaggcgca agctatgagc tcaggggtga gtaacagcct ggcatcaatg cagtcaaact
    16201 caactaaagc agcctctggt ctgtctaata gtgtgacaag cgaaatgtct tctgcagcaa
    16261 cctctgcaac ctcaagcgtt aataggttgt cctcggcggt cgagtctggc ttcaataagg
    16321 cgaagacttc agcaacaacc tcaatgaatg ggatagccaa tgcggttaaa acaggattta
    16381 gtagcattaa cagtactgca aagcaatcta tggcaaacct tgtaagttca gtgacttctg
    16441 ggatgtctcg tgcaaccgcg gtgtctaata atgcttgtca gaagatcctc tcaatattca
    16501 gggctttggc agggcagatg tcttctgcag gagcttatgc aggtcaaggt tttgctaatg
    16561 gtctagctag tagcgcaggc acaatctacg caatagctaa cagcattgcc gctaatgtag
    16621 cagcaacaat tagacgcgct ttagatattc actcaccatc tcgtgtcact aaaacactag
    16681 gtgcatttac tggagagggg tttgccctcg gtatggcaga gtggataggt gagatcaata
    16741 gcctaggtaa agcatacgca acagcagtta ctgatcaaaa ctggggggta aatagtactg
    16801 tttcaacatc tgctaaagtt aataatagtg gaatcaatac ctctcttgat aatcttagcg
    16861 aagaggttag gcagtctcaa ttgtcagagc ctatctttga agtgcataat gagattgttg
    16921 gagataaaat ttacacggct gttaaagaaa aagaatcaag agagcaatct aaagattctt
    16981 attttgtttt tgactagaag ggagtatttt ggatttatta atagaaaaag agagtcaggc
    17041 aactaggttg tctgactttg gtatttataa tattgctatt gaagatagcg ctcctttact
    17101 atctgtatca caccgagcag tcaaaggaag gagtggttac atctacgatg gggctacttt
    17161 tacgactaaa acgcttaagg tcaaaggaag agtgacgata agcaacgtag aaagactttt
    17221 ggacaaacag gacgaactaa acgctctgct tgttgttgac gaacctttct acgtgactaa
    17281 gatgtaccct gaaaactcag atctatttaa ctttgagctt ccaggggaaa gcacaggaga
    17341 cctgcaactt attggctctc ctcataaacc ttggaaatat agatttaaag tcatcttaga
    17401 cgacactatc gcttatgaat ttataggaaa aaccagtcaa ggactaaaat ataatctttc
    17461 cttcgcgcta agaactgctg aattaccttt cggtgagaca aagccaaagg acataactct
    17521 atcagggggc agttttgcct atgcaggaac cgctaaagca agtcagctag agtggccttt
    17581 tattatagag cttactcctt ctggaggtca gactaatttt tatattgaaa ttgatggcag
    17641 gcgctttgag tttaagcaga atagccagct acaaaatagt gataaattac ttttgacagg
    17701 catagctacc acgctaaacg gaaattatgt caatgctaaa acgaactatg agtattttat
    17761 cttcaatcct aatcctaaca agagaattac ttacaaaacg gattttcttg gcacgattag
    17821 gattttaaat tttgtagagt tgtataaata gggaggtcta atgattactt ttttggatca
    17881 tcgtgacatt gagtatgggg tcattagtgt cattaggcat actaatgctg ttaatggtga
    17941 gcgctcagtg agcggggaaa tttatacaaa cagtgatgtc ctcaataata tcgatagagg
    18001 gtggcgactt agatttgaag atgagtatta cgttgttatt tacgcaaagc ctgttgatgt
    18061 tggtcaaaaa acgcaagttt cgtttgatgc catacaccaa tttttttggg acttcagcaa
    18121 gagtagtatc tatgagggtc tcggagatgg gtctcatacc attgatacct atctagagac
    18181 agtatttaaa ggtagtgggt accgctataa gctagaagta ggtgtgaacg catttagaaa
    18241 acaatctttt aattataagt ccaggttaga tttattcaat gagattatta aagcgaccgg
    18301 acttgaattt tcagtcagcg ggaaagttgt tcggctacta aaaaacatag gtactgatct
    18361 gtcaacagtg gtacgaaaaa atttcaacat gaatgacctt accattgaaa aaaatattga
    18421 tagctttatt acctatcaga aaggctttgg tgcatggacc gacccagagg accactctaa
    18481 ggggagactt gaggcggaat acgaaagtcc tcttgccaag gagtacggtc gacttgaagg
    18541 ggcgcctctg acggatgagc gcttcacggt ggcagataat ctaaaagaag ctttaaaaag
    18601 taatgttgaa aattcatata agatatcagt aaaaattgat atggaagatt taacgagagc
    18661 tgggtataga tgtgagaggc cggttgctgg tgactacatt atggcgataa atgagacttt
    18721 aggttttcaa gagcgtatac ggatagtttc ttttacaagc tactacgacg caactggagc
    18781 tttagtaaaa catgaagtca cttgtaatga tatcggatct gtgaaaaaac aaagcgtagg
    18841 aagtctgtct ataaacagta gaatcaatca gatagatgca gatattgctt ctgcaattga
    18901 agtagcaaca caagcactag tatctgctga cgggaaaaat actgtttacg gtggcacaga
    18961 aatgcccaaa gatgaaccaa aaggaacctt aaaaaaagga gacatattat tcctaaaagt
    19021 tggcgatact actagaatgt atttctggaa tggtgctgag tgggaagaac ctgaggttgt
    19081 gaacgaccca gaacgatggc gtgaagacct cgaaaaacaa atctctgagg cgatcgaaaa
    19141 agccaaaaaa gcacaggaag aaatcaacca acgcaccgat aaagagcttg aagaatttag
    19201 agccgccctc aaaaacctag cgttaccaga ggaagcgatt aaaaaaatca ccgaggctat
    19261 aaaagttgat gacatcccgt ctattaaaca aagctttgat gacctcaaaa atagagtgag
    19321 tgagacaagc gaagaatctc gtttaactgc cgaaatttta ggaaataacg gtaagacccg
    19381 ctataacaaa aatttattgg ttggcgaccc taaccgtgta aaaacctatg accaagacta
    19441 tatcgaggta gaagctaatg atggcggctt taagcgtggc gagacctaca cgattagctt
    19501 tagtcaaacg tgtgagctac tcaaagaagt agctatcaca ttgacgcaaa ttaacaacaa
    19561 gggagttaag ttagtactga caccaaccaa agcaaaaatg gatgcacaga cgtttgaggt
    19621 cactaaggat aaacagtcta tagaggtata tcctttaagt tatacaggcg ttttaactgg
    19681 tgactggtat aaacctaagc aaatagattt aaacgcctca gacacgcagg aattggctct
    19741 agagatggat tataaagatg ttgttgacgg caaggatgcc acaataacag gggcatggtc
    19801 agacagtcca caaataattt tatacggagg taaaaaatga cagaaactat accattaaga
    19861 gtccaattta agcggatgaa agccgccgag tgggcaagta gtgatgtcgt cttactggag
    19921 ggtgagattg gctttgagac tgacactggt tttgctaagt ttggcgatgg tcaaaacact
    19981 tttagtaagc ttaagtacct tactggtccc aaaggtccta aaggagacac tggtctccaa
    20041 ggtaaaactg gaggaactgg tcctcggggc cctgctggca agcctggaac gacagattat
    20101 gatcaactcc aaaataaacc agatctaggt gcgtttgcac aaaaagaaga aactaatagt
    20161 aaaatcacca aattagaatc aagcaaagca gataaaaacg ctgtttactt aaaagcagag
    20221 tcaaaaatag agcttgacaa gaaattaaat ctcaaaggcg gcgtcatgac aggtcaacta
    20281 cagtttaaac ctaataaaag tggtattaaa ccctcatctt ccgtaggagg agcgattaac
    20341 attgatatgt ctaaatcgga aggtgctggt gttgttgtct attctaacaa tgataccagt
    20401 gatgggccgt taatgagctt gcggacgggt aaagagacct tcaatcaatc agcgcttttt
    20461 gtcgattaca gcggtaagac taatgccgtt aatattgcga tgcgtcagcc aaccaccccc
    20521 aatttttcct ctgcgcttaa tattactagc ggcaatgaaa atggtagtgc gatgcaaatt
    20581 agaggcgtcg aaaaagcatt gggaacgctc aaaatcacac acgaaaaccc aagcgttgag
    20641 gcaaattacg atgaaaacgc tgcagcgtta tctattgata tcgttaaaaa acagaaaggc
    20701 ggaaaaggta ctgctgctca aggaatctac attaactcaa cctcaggaac gacagggaag
    20761 ttgcttagga ttagaaatct tggtgatgat aagttctacg tcaagtctga cggtggtttt
    20821 tatgccaaga aaacttcgca gattgatggc aacctgaaac tcaaggaccc cacagcgaat
    20881 gatcatgcgg caaccaaagc ttatgtagat aaagcaatt
//
LOCUS       DAUCLR010000027        20170 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.027, whole genome shotgun sequence.
ACCESSION   DAUCLR010000027 DAUCLR010000000
VERSION     DAUCLR010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20170
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.027"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(194..1648)
                     /locus_tag="VGT87_000837"
     CDS             complement(194..1648)
                     /locus_tag="VGT87_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ3813710.1"
                     /translation="MERLGGADLDYCYHNHHDTSMISAEQLVFTYHDQKNPACQVPTC
                     QIASGQFIVLCGPSGSGKSTFLKLLNGIIPDYYAGKYEGRLDVADCQAGRDSVETFSR
                     SVASVFQNPASQFFYREVQHELVFPCENQGLDAKVIMKRLWTLAEDFAFAELLNKDMF
                     GLSGGQKQRVAIATAIMQGTNIMLFDEPTANLDSAGIAAVKAYLTQLKAAGKTIIVAE
                     HRLHYLMDLADNFFYFKNGRLTDKLTTQDLLALTDEQRQDMGLRRLDLSDLKPVLAGK
                     IESQHYRPDDSLCIEHLTVRAGSKILRCIEQLSFAAGSISGITGSNGLGKSQLVYYIA
                     GILDDKKATIKFQGIPLSAKQRLSKTSIVLQEVSLQLFAESVSKEVNLGHERHPRTTE
                     VIERLSLTTLLERHPASLSGGEQQRVMIAASLLADKDILIFDEPSSGLDLLQMKALAN
                     LLMQLKTQHKVVILISHDEELLSSVCDTIYQMLP"
     gene            complement(1585..2265)
                     /locus_tag="VGT87_000838"
     CDS             complement(1585..2265)
                     /locus_tag="VGT87_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEQ3813711.1"
                     /translation="MRLDVRTKLLLLVLANACFFFRVDGFLEFIIVIFLLLLLSALNK
                     KKLAFKLAVVYLLMIGLSVIPLSIFPSYLDHLLSFVSIAGRLVFPSLLAGLITIKTTT
                     IYELVHGLRKWHFPEVWLLTLAVMCRFIPMIRQECCVIHRSLTIRGIILTKWSILIRP
                     KQYLEYLMVPLLLSLIRSSQELTIASLTKGLAVNKGTSECFSSHLTWKDWGVQIWITV
                     IIIITILQ"
     gene            complement(2262..2855)
                     /locus_tag="VGT87_000839"
     CDS             complement(2262..2855)
                     /locus_tag="VGT87_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEQ3813712.1"
                     /translation="MKQLTIKDIMITGAFAALYFICVGLGTFINIFFDRSGNMLYAPA
                     FAALLGGTIYFLLIAKLQTFGPITLLGLMMGGFFFLSGHFFAAMFPSIIFGLIADIIA
                     SLGKYKSRFFNDLSYMVFAFVNSGPILLMWLLKDAYVESLLARGKSMTYVNRIMYDFS
                     LGHVIWFVGIVLAGALLGSAFAHYLLKKHFIKSGLLS"
     gene            complement(2852..4522)
                     /locus_tag="VGT87_000840"
     CDS             complement(2852..4522)
                     /locus_tag="VGT87_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ3813713.1"
                     /translation="MAKPNRSTLSLVWRLLTLMKTLLPWIILAVGFAVMGFVITVSIP
                     TGIAYLGLLAIRQEVIPILALYLLIALAFLRGFVRYGEHYFGHFVAFHSLAAFRNLIF
                     KKLRALSPACLDSQDSGYLLKMIGEDIEALEVFFAHTIAPICTAILSAGLMFWYFCQS
                     SWQLALLALATYACLAIVIPIYFANILQVLLKSQNEGRKDYLSYFLESLRSVKDLLQF
                     QVLDEQFERLIKKSNHVNALDRNVAQAQFLQMALTFFWLGLMILAFSYMVFDGICHDS
                     LSFDKGLLTFIAFTASFSPFLELGRLPLGFKRAMNAARNIFDLLDENVIVDEGTKQID
                     RLRSVSFEDLSFAYPKRQELVFKDLSVTFQEKGIIGIKGESGSGKSTLVKLIMKWYNW
                     RTGDIFLNDRNSCLLNAAKLQDTIAYVPQTAQLFQQSIRENLTFGRQDISDESIWNLA
                     EACGMKDRLLACKEGLDTIIKSPSDFSAGEGQRLELMRALLKDASCYIFDEPTSNLDS
                     LNEAILLDLIKTHCQGLVFLISHRPSTLACADHLFCVKNGFLKEVNKK"
     gene            complement(4515..6278)
                     /locus_tag="VGT87_000841"
     CDS             complement(4515..6278)
                     /locus_tag="VGT87_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEQ3813714.1"
                     /translation="MMETEDTISRGKRKRLLKRLRERIAPKRYLLYVSAFLSWLQFVM
                     RMISFYLIAKTFSTFILGHAIALGRLAGLLLLLNVVGFVLAILGKQLQGIASQFARDS
                     LKQSFFEAFIDLDGQFDAHASDADILTLASQGIDSLDTYYGYYLSLSMRTKWNCTTIM
                     ILVFLIYPLAGLVFLGVLPLIPLSIVAMQKRSKQIMSHYWSSYMDVGNLFMDDLKGLN
                     TLYSYQATERYEQEFSGKAEQFRKATMSLLGFQLQAVGYMDAVMYLGIGLSGFLAVQA
                     LATGQLSFFNFLFFLLIATEFFTPIREQGYGMHLVMMNTKMADRIFSFLDSVPARKDN
                     KSKTAINFNQIDIQNISLAYEKKTVLSGVTMTLTKGQLTAIAGVSGQGKTSLAQLLLK
                     RQSATTGHILFDGLDSDNLSQEIINQQVLYVSDQSTLLNRSIYDNLRLAANLSKKEIL
                     DWIDQHGLLSFINWLPDGLDTIVGENGNLLSPGQKQQVICARALLSKRSLYIFDEATS
                     SLDAENERIIDNLITRLAKTAIVIVITHKMSRLKGANQVLFLSTGQPACLGKPCDLYR
                     DQPTYRHLVDTQARLEASIYG"
     gene            complement(6275..7111)
                     /locus_tag="VGT87_000842"
     CDS             complement(6275..7111)
                     /locus_tag="VGT87_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ3813715.1"
                     /translation="MSKNSSHIQVQNLSFAYEEKLVLDDLTFSIPKGKITTIMGANGS
                     GKSTLLQLLTKNLPLKQGQVWLEQEELATISLKAFAKKVAVVHQYHQVVEGLLVGELV
                     AMARLARRSFFKTTTKEDEERLAWALEVTALSDYVYRDIQHLSGGEQQRVWIAMALAQ
                     DTDIIFLDEPTTYLDIKYQLDILKLITKINQELGLTIVLVLHDINQALSLSDHLIGLK
                     DGRLYAQGQPRDLLDQQFIRDVFGVELPFVERNGQQFVLTSLVNETNDIRTQRRDRNK
                     NR"
     gene            complement(7108..8130)
                     /locus_tag="VGT87_000843"
     CDS             complement(7108..8130)
                     /locus_tag="VGT87_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEQ3813716.1"
                     /translation="MTASDLQRDQSLLAKKRFRSGLYLVLLILSLLFLAVIALSLGGL
                     AVSYGAIVKGLFVAYDPQVALIYDLRFPRIVIALLAGAGIAVSGVLFQAVLKNPISDP
                     AIIGICSGASFMVLVSSLLLPQLLLYGPIVSFLGGGVSFLLIYGLAWKKGLNPIRLIL
                     TGIAINALFMGLSTALTSFFTSASPMVNALLAGHISQKTWADVGVLFPYTFIGLLLAL
                     LLSKTCNLLLLDDQVIRHLGIDATALRLGISLVAVLLASVATSIVGVVSFLGLIVPHM
                     SRLLVGSKHQILIPFSALLGAFVFLLADTLGRSLAYPLEISPAIIMSIVGGPYFIYLL
                     RRSDII"
     gene            complement(8132..9016)
                     /gene="isdE"
                     /locus_tag="VGT87_000844"
     CDS             complement(8132..9016)
                     /gene="isdE"
                     /locus_tag="VGT87_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HEQ3813717.1"
                     /translation="MIKRCKGIGLALMVFFLVACVNQHSKTAKETEQQRIVATSVAVV
                     DICDRLNLDLVGVCDSKLYTLPKRYDAVKRVGLPMNPDIELIASLKPTWILSPNSLQE
                     DLEPKYQKLDTEYGFLNLRSVEGMYQSIDDLGNLFQRQQEAKELRQQYQDYYRAFQAK
                     RKGKKKPKVLILMGLPGSYLVATNQSYVGNLLDLAGGENVYQSDEKEFLSVNPEDMLA
                     KEPDLILRTAHAIPDKVKVMFDKEFAENDIWKHFTAVKEGKVYDLDNTLFGMSAKLNY
                     PEALDTLTQLFDHVGDHP"
     gene            complement(9000..9875)
                     /locus_tag="VGT87_000845"
     CDS             complement(9000..9875)
                     /locus_tag="VGT87_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEQ3813718.1"
                     /translation="MTKVVIKQLLQVIVVFMISLSTMTGVVYADKGQIYGCIIQRNYR
                     HPISGQIEDSGGEHSFDIGQGMVEGTVYSDAMLEVSDAGKIVLTFRMSLADYSGNYQF
                     WIQPGGTGSFQAVDYNITQKGTDTNGTTLDIAISLPTVNSIIRGSMFVEPMGREVVFY
                     LSASELIQKYSGNMLAQLVTETDNSQNQEVKDSQKPVDTKLGESQDESHTGAMITQNK
                     PKANSSNNKSLSDKKILPSKMGLTTSLELKKEDKFRSKKDLSIMIYYFPTFFLMLGGF
                     AVWVWKKRKKNDKTM"
     gene            complement(10072..13899)
                     /gene="shr"
                     /locus_tag="VGT87_000846"
     CDS             complement(10072..13899)
                     /gene="shr"
                     /locus_tag="VGT87_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein Shr"
                     /protein_id="HEQ3813719.1"
                     /translation="MKKISKCAFVAISTLVLIQATQTVKSQEPLVQSQLVTTVALTQD
                     NRLLVEEIGPYASQSAGKEYYKHIEKVIVDNDVYEKSLEGERTFDINYQGIKINANLI
                     KDGKHELTIVNKKDGDILITFIKKGDKVTFISAQKLGTTDHQDSLKKDVLSDKPVPQN
                     QGTQKVVKSGKNTANLSLITKLSQEDGAILFPEIDRYSDNKQIKALTQQITKVTVNGT
                     VYKDLISDSVKDTNGWVSNMTGLHLGTKAFKDGENTIVISSKGFEDVIITVTKKDGQI
                     HFVSAKQKQHVTAEDRQSTKLDVTTLEKAIKEADAIIAKESNKDAVKDLAEKLQVIKD
                     SYKEIKDSKLLADTHRLLKDTIESYQAGEVSINNLTEGTYTLNFKANKENSEESSMLQ
                     GAFDKRAKLVVKADGTMEISMLNTALGQFLIDFSIESKGTYPAAVRKQVGQKDINGSY
                     IRSEFTMPIDDLDKLHKGAVLVSAMGGQESDLNHYDKYTKLDMTFSKTVTKGWSGYQV
                     ETDDKEKGVGTERLEKVLVKLGKDLDGDGKLSKTELEQIRGELRLDHYELTDISLLKH
                     AKNITELHLDGNQITEIPKELFSQMKQLRFLNLRSNHLTYLDKDTFKSNAQLRELYLS
                     SNFIHSLEGGLFQSLHHLEQLDLSKNRIGRLCDNPFEELSRLTSLGFAENSLEEIPEK
                     ALEPLTSLNFIDLSQNNLALLPKTIEKLRALSTIVASRNHITRIDNISFKNLPKLSVL
                     DLSTNEISNLPNGIFKQNNQLTKLDFFNNLLTQVEESVFPDVETLNLDVKFNQIKSVS
                     PKVRALIGQHKLTPQKHIAKLEASLDGEKIKYHQAFSLLDLYYWEQKTNSAIDKELVS
                     IEEYQQLLQEKGSDTVSLLNDMQVDWSIVIQLQKKSSNGQYVTVDEKLLSNDPKDDLT
                     GEFSLKDPGTYRIRKALITKKFATQKEHIYLTSNDILVAKGPHSHQKDLVENGLRALN
                     QKQLRDGIYYLNASMLKTDLASESMSNKAINHRVTLVVKKGVSYLEVEFRGIKVGKML
                     GYLGELSYFVDGYQRDLAGKPVGRTKKAEVVSYFTDVTGLPLADRYGKNYPKVLRMKL
                     IEQAKKDGLVPLQVFVPIMDAISKGSGLQTVFMRLDWASLTTEKAKVVKETNNPQENS
                     HLTSTDQLKGPQNRQQEKTPTSPPSAATGIANLTDLLAKKATGQSTQETSKTDDTDKA
                     EKLKQLVRDHQTSIEGKTAKDTKTKKSDKKHRSNQQSNGEESSSRYHLIAGLSSFMIV
                     ALGFIIGRKTLFK"
     gene            complement(14389..15918)
                     /locus_tag="VGT87_000847"
     CDS             complement(14389..15918)
                     /locus_tag="VGT87_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEQ3813720.1"
                     /translation="MNKNKLLRVAMLLSLLAPTAESMTVLAQDVMLETHKATTNETSD
                     SSSKEENNKNAAPTTSDKTDQGPLDASAETNSNSLVNADDKKRSDSSQSAVGSSDNKA
                     EAENQVDDKSTDHSKPTDHSKPTDQPKPTDQPKPSPSKVDTAPASSLSRQLPEVRTPI
                     QSLSPYVSDLDLSEIDIPSVNTYAAYVEHWSGKNAYTHHLLSRRYGIKADQIDSYLKS
                     TGIAYDSTRINGEKLLQWEKKSGLDVRAIVAIAMSESSLGTQGIATLLGANMFGYAAF
                     DLDPTQASKFNDDSAIVKMTQDTIIKNKNSNFALQDLKAAKFSRGQLNFASDGGVYFT
                     DTTGSGKRRAQIMEDLDKWIDDHGGTPAIPAELKVQSSASFASVPAGYKLSKSYDVLG
                     YQASSYSWGQCTWYVYNRAKELGYQFDPFMGNGGDWKYKAGYALSKTPKVGYAISFAP
                     GQAGADGTYGHVSIVEDVRKDGSILISESNCIGLGKISYRTFTAQQAEQLTYVIGKSK
                     N"
     gene            complement(16005..17105)
                     /locus_tag="VGT87_000848"
     CDS             complement(16005..17105)
                     /locus_tag="VGT87_000848"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEQ3813721.1"
                     /translation="MISSFHRPTVARVNLQAIKENVASVQKHIPLGVKTYAVVKADAY
                     GHGAVQVSKALLPQVDGYCVSNLDEALQLRQAGIDKEILILGVLLPNELELAVANAIT
                     VTIASLDWIALARLEKKECQGLKVHVKVDSGMGRIGLRSSKEVNLLIDSLKELGADVE
                     GIFTHFATADEADDTKFNQQLQFFKKLIAGLEDKPRLVHASNSATSIWHSDTIFNAVR
                     LGIVSYGLNPSGSDLSLPFPLQEALSLESSLVHVKMISAGDTVGYGATYTAKKSEYVG
                     TVPIGYADGWTRNMQGFSVLVDGQFCEIIGRVSMDQLTIRLPKAYPLGTKVTLIGSNQ
                     QKNISTTDIANYRNTINYEVLCLLSDRIPRIY"
     gene            complement(17102..17458)
                     /locus_tag="VGT87_000849"
     CDS             complement(17102..17458)
                     /locus_tag="VGT87_000849"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEQ3813722.1"
                     /translation="MIVGHGIDLQEISAIEKVYQRNPRFAQKILTEQELAIFESFPYK
                     RRLSYLAGRWSGKEAFAKAIGTGIGRLTFQDIEILNDVRGCPILTKSPFKGNSFISIS
                     HSGNYVQASVILEDKK"
     gene            complement(17574..20093)
                     /gene="secA"
                     /locus_tag="VGT87_000850"
     CDS             complement(17574..20093)
                     /gene="secA"
                     /locus_tag="VGT87_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEQ3813723.1"
                     /translation="MANILRKVIENDKGELRKLEKIAKKVESYADQMASLSDRDLQGK
                     TLEFKERYQKGETLEQLLPEAFAVVREAAKRVLGLFPYRVQIMGGIVLHNGDVPEMRT
                     GEGKTLTATMPVYLNAIAGEGVHVITVNEYLSTRDATEMGEVYSWLGLSVGINLAAKS
                     PAEKREAYNCDITYSTNSEVGFDYLRDNMVVRQEDMVQRPLNFALVDEVDSVLIDEAR
                     TPLIVSGAVSSETNQLYIRADMFVKTLTSVDYVIDVPTKTIGLSDSGIDKAESYFNLS
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEDGEILIVDQFTGRTMEGRRFSDGL
                     HQAIEAKEGVRIQEESKTSASITYQNMFRMYKKLAGMTGTAKTEEEEFREVYNMRIIP
                     IPTNRPIARIDHTDLLYPTLESKFRAVVEDVKTRHAKGQPILVGTVAVETSDLISRKL
                     VEAGIPHEVLNAKNHFKEAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMRRFGSDRIKAFLDRMKLDE
                     EDTVIKSGMLGRQVESAQKRVEGNNYDTRKQVLQYDDVMREQREIIYANRRDVITANR
                     DLGPEIKAMIKRTIDRAVDAHARSNRKDAIDAIVTFARTSLVPEESISAKELRGLKDD
                     QIKEKLYQRALAIYDQQLSKLRDQEAIIEFQKVLILMIVDNKWTEHIDALDQLRNAVG
                     LRGYAQNNPVVEYQAEGFKMFQDMIGAIEFDVTRTMMKAQIHEQERERASQRATTAAP
                     QNIQSQQSANTDDLPKVERNEACPCGSGKKFKNCHGRKSFS"
BASE COUNT         6231 a         4189 c         3461 g         6289 t
ORIGIN      
        1 caaaaagcat ctgttttggc tcactttata tgtaaaaatc ggaaaccgaa tgtcaaatca
       61 gcagagctat gcatgctttt tgaacataaa actggggttg tctacgttct gactcttgta
      121 tctattatac aagagttcct atttattttc agataaaagc cttttagtac ttttaaacag
      181 ttctagggga tcgttaaggt agcatttgat aaatggtatc gcagacacta cttagaagtt
      241 cttcatcatg tgaaatgagg atgacaacct tatgttgggt cttcagctgc atgagaagat
      301 tagctaaagc cttcatttgt aacaaatcaa gtccgctact tggctcatca aaaattaaga
      361 tatctttatc agccaaaaga ctagcggcaa tcatcactct ttgttgctca cctcctgaca
      421 aactagctgg atgacgttct aaaagagtcg ttaaagataa ccgctctatt acttctgtcg
      481 tccgtggatg tcgttcatgg cccaagttga cttcctttga aaccgattct gcaaatagtt
      541 gcaaagaaac ttcttgcaag acaatactag tctttgataa acgttgcttg gcagataaag
      601 gtatgccttg aaacttaata gttgcttttt tatcgtctaa gataccagct atataataaa
      661 ctaattgcga ttttccaagc ccgtttgatc ctgtaatgcc agaaatactt cctgcagcaa
      721 aactaagctg ttcaatacat ctaagtattt tgcttcccgc tctaactgtt agatgctcaa
      781 tacacaggct atcatctggg cgataatgtt gactctcaat cttaccagct aacacaggtt
      841 ttaggtcaga caagtctaag cgacgtagtc ccatatcttg ccgttgttca tctgtcaagg
      901 ctagcaaatc ttgtgttgtt aacttatctg ttaatctacc atttttaaag tagaaaaagt
      961 tatctgccaa atccatcaag taatgtaaac gatgctcggc tacaataatg gtttttcctg
     1021 ctgcttttaa ttgggtaaga taggctttaa cagcagctat gcctgcacta tctaagttag
     1081 cagttggctc atcaaaaagc ataatattag taccttgcat aatggccgtt gcaatagcaa
     1141 ctctttgttt ttgccctcct gacaatccaa acatatcctt gttaaggagc tctgcaaaag
     1201 caaaatcttc tgctaaagtc caaagacgct tcatgataac ctttgcatct agaccttgat
     1261 tttcacaggg aaagaccagt tcgtgttgga cttcgcgata gaaaaactga ctggctggat
     1321 tttgaaaaac agaggccaca gatctagaaa atgtttcaac actgtctctg cctgcttggc
     1381 aatcagccac atctagtcta ccttcatatt ttccagcata ataatcagga attatgccat
     1441 taagaagttt taaaaaggta gatttacctg aaccagaagg tccacacaaa acaatgaatt
     1501 gtccagatgc aatttgacaa gtcggtacct gacaagctgg atttttttga tcatgatagg
     1561 taaaaacaag ttgttctgct gaaatcattg aagtatcgtg atgattatga taacagtaat
     1621 ccaaatctgc accccccaat ctttccatgt taagtgtgag ctaaagcatt cactggtgcc
     1681 tttgttgact gctagccctt tagttagact agcgatagtt agttcttggc tacttcttat
     1741 caaagataaa agcaatggaa ccattaaata ctccaagtat tgtttaggtc tgatcaaaat
     1801 agaccatttt gttaaaataa tccccctgat tgttaaagag cgatgaataa cacaacattc
     1861 ttgtctaatc attggtatga aacggcacat aacggctaag gtcaatagcc agacttctgg
     1921 gaaatgccat ttgcgtaatc catgaaccaa ttcgtagatg gtagttgtct taatagtaat
     1981 taaacctgct agtaaagaag ggaaaactaa ccttcctgct attgagacaa aactcaataa
     2041 gtgatcaaga taagaaggga aaatagatag cgggattaca gatagaccga tcattaacag
     2101 ataaacaacc gccaacttga acgctagctt tttcttattt aaagcagaca aaagtaataa
     2161 gagaaaaata acaattataa attctaaaaa gccatcaaca cgaaaaaaga agcaggcatt
     2221 ggcaagtacc aacaaaagca gtttggtgcg aacatcaagc ctcatgataa caatcctgat
     2281 ttgataaaat gtttctttag taaataatgt gcaaaagctg accctaataa ggctcctgca
     2341 agaacaattc ctacaaacca aataacatgc cctaaactaa aatcatacat aatgcgattg
     2401 acataggtca tacttttacc gcgggctaac aaactttcaa cataggcatc tttcaacaac
     2461 cacatcagca aaatagggcc actattgaca aaagcaaaaa ccatataaga taggtcatta
     2521 aaaaaccttg acttgtattt tcctaaagaa gcaataatat ccgctattaa accgaaaata
     2581 atgctgggaa acatcgcggc aaaaaagtga ccactaagga agaaaaaacc tcccatcatc
     2641 agtcctaata atgttattgg cccaaatgtt tgcaatttag caatcaaaag aaaataaatt
     2701 gttcccccca gtaaagctgc aaaggcaggg gcatataaca tatttcctga gcgatcaaaa
     2761 aagatgttaa taaacgtgcc tagaccaaca caaataaagt agagcgcggc aaatgcgcct
     2821 gttatcataa tatcttttat tgttaactgt ttcatttttt attcacctct tttaggaaac
     2881 cattttttac gcaaaataga tgatcagcac aagctagggt tgatggcctg tgagaaatta
     2941 agaagaccaa tccttgacaa tgtgttttaa ttaaatcaag caagatagct tcatttagag
     3001 aatctaaatt tgacgtgggt tcatcaaaaa tatagcaact cgcatctttc aataaagctc
     3061 gcatcaactc taaacgttga ccttctccag ctgaaaagtc agaaggactc ttgataatcg
     3121 tatccaaacc ttccttacaa gctaacagtc tgtctttcat accacaagcc tcagctaaat
     3181 tccaaatgga ctcatctgaa atatcttgac ggccaaaggt aagattttca cggatagact
     3241 gttggaaaag ctgcgctgtt tgtggcacat aagcaatagt atcttgtaat ttagcagcat
     3301 ttagtaagca actattccta tcattaagaa aaatatctcc tgtcctccaa ttataccatt
     3361 tcataattaa tttcaccaaa gttgatttac cagatcctga ttcgccttta atgccaataa
     3421 tccccttttc ttgaaaggta actgataaat ccttaaaaac cagctcttgg cgctttggat
     3481 aagcaaaact gaggtcctca aaagaaacac tccttaaacg atctatctgt tttgtgcctt
     3541 cgtctactat gacattttcg tctagaaggt caaaaatatt tcgagccgca ttcatagcac
     3601 gcttaaagcc taatggcaaa cgtcctagtt ctaaaaaagg agaaaacgaa gcagtgaaag
     3661 caataaaagt taataagcct ttatcaaatg ataagctgtc atggcatatc ccatcaaaaa
     3721 ccatataaga aaatgctaag atcattaagc caagccagaa aaaagtcaat gccatttgca
     3781 aaaattgagc ttgcgctacg ttacgatcaa gagcatttac atgattactt ttttttatta
     3841 atcgttcaaa ttgctcgtct aatacctgaa attgcaaaag atctttgact gatcgtaaac
     3901 tctctaaaaa ataagacagg taatcttttc gaccttcatt ttggcttttt agtaaaacct
     3961 gtaaaatatt agcaaaataa atgggtataa caatggctaa acaagcatag gttgccaaag
     4021 ctaataaggc taactgccaa ctactttgac aaaagtacca gaacattaaa cctgcagaca
     4081 atattgctgt acaaataggc gcgattgtgt gggcaaaaaa aacttctaat gcctcaatat
     4141 cttctccaat cattttaagc aaataaccac tgtcttgact atccaagcaa gctggagaaa
     4201 gagctcttag tttcttaaag atcaagttcc taaaagctgc taaactatga aaagcaacaa
     4261 aatgaccaaa ataatgctct ccataacgaa caaaacctct taaaaaggct agagcaatca
     4321 aaagatataa agctaatatt ggaatcactt cttgtctaat agctagtagt cctaaataag
     4381 ctattccagt tggaatactt accgtgatga caaaacccat aacagcaaag ccaacagcca
     4441 agataatcca aggtagcaga gttttcatta gggttaataa acgccaaacc aggcttaagg
     4501 tagatctgtt aggtttagcc ataaatactt gcctccagtc ttgcttgagt atcaactagg
     4561 tgacgataag ttggttggtc ccgataaagg tcgcaaggtt tgcctaaaca agcaggctgc
     4621 cctgtgctta aaaagagtac ttggttagct cctttcagtc gagacatctt atgagtaatc
     4681 acaataacta ttgccgtctt agctaatctc gttattaaat tgtcaataat acgttcattt
     4741 tctgcatcta gactagatgt tgcctcatca aagatataaa gagacctctt acttaataga
     4801 gctcttgcgc aaatcacctg ttgcttttgg cctggtgata acaggttacc attttcacca
     4861 actattgtgt caaggccatc tggtagccag ttgatgaagc ttaacaaacc atgttgatct
     4921 atccaatcta agatttcttt tttagagaga tttgcggcta accttaaatt atcgtaaatg
     4981 ctacggttca ataaggtaga ttggtccgat acataaagaa cctgctggtt gataatttct
     5041 tgcgacaaat tgtcactgtc taaaccgtca aataaaatat gcccagtcgt tgcagactgg
     5101 cgtttcaaga ggagttgagc tagacttgtt tttccttgtc ctgacacccc cgcgatagct
     5161 gttagttggc ccttcgttaa tgtcatggta accccactaa gcactgtttt tttctcataa
     5221 gctaaagaaa tattttggat gtcaatctgg ttaaagttta ttgctgtttt actcttgtta
     5281 tctttccttg ctggaacact atccaagaaa ctaaagatac ggtcagccat tttggtattc
     5341 atcatgacca agtgcatgcc ataaccctgc tcacgaatcg gtgtaaaaaa ttctgtagca
     5401 atcaagagaa aaaagaggaa attaaaaaaa gataactgac ctgttgccaa agcttgaacg
     5461 gctaaaaaac ctgacaaacc aattcccaga tacataactg catccatata accaactgct
     5521 tgtaactgaa agccaagcaa agacatggta gcctttcgaa actgttctgc cttacccgaa
     5581 aattcttgct cgtaacgttc tgttgcttga taagaataca gtgtattaag accttttaag
     5641 tcatccataa ataaattacc aacatccata taagaagacc aataatgact cataatttgc
     5701 ttagaccgct tttgcatagc aacgattgaa agtggaatca gaggcagaac tcctaaaaag
     5761 acaaggcctg ccaatggata aattaaaaag actagtatca taatagttgt gcagttccac
     5821 ttggttctca tagacaaaga caaatagtaa ccataataag tatccaaact atcaatcccc
     5881 tgtgaagcca acgttaaaat atcagcatca ctggcatgag catcaaattg cccgtctaaa
     5941 tcaataaaag cctcaaagaa agattgtttg agtgaatctc tagcaaattg tgaagcgatc
     6001 ccttgcagtt gcttgcctaa tatagcaaga acaaagccta ccacatttaa aagtaggagt
     6061 aaacctgcca agcgtcctaa ggcaatagca tgacccaaaa taaaggtcga aaacgttttg
     6121 gcaatgaggt aaaaactgat catcctcatc acaaattgta accaagataa aaaggctgac
     6181 acataaagca aatacctttt tggtgcaatt cgttcacgta atcgttttaa cagtcttttc
     6241 ctttttcccc ttgatattgt atcttctgtt tccatcatct gttcttgttc ctatctctcc
     6301 tttgagttct aatatcattg gtttcattga ccaatgaagt gaggacaaac tgttgaccat
     6361 ttctctcaac aaagggcaat tcaacaccaa agacatctct gataaactgt tgatcaagga
     6421 ggtctcttgg ttgcccttgg gcataaagtc gcccatcttt cagtccaata agatggtctg
     6481 aaagagacaa ggcctgattg atgtcatgta atactaacac aatcgttaag cctagctctt
     6541 ggttgatttt agtaatcagc ttgagaatgt caagctggta cttaatatcc aaataagtcg
     6601 ttggctcatc taaaaaaata atatctgtat cctgggcaag ggccatagca atccaaaccc
     6661 tctgctgttc accaccagaa aggtgttgga tgtcacgata aacataatca ctcaaagccg
     6721 ttacttctaa ggcccaagca aggcgttcct catcttcttt ggtagtagtt ttaaaaaaag
     6781 aacgacgggc aagcctagcc atggctacca attcacccac taacaaacct tcgaccactt
     6841 gatggtattg gtgtaccact gccacttttt tcgcaaaagc cttgagagaa atcgttgcta
     6901 attcttcctg ctcaagccaa acctgccctt gttttaacgg aaggtttttg gttaataatt
     6961 gtaataaggt tgacttgcca ctgccatttg cccccataat agtggtaatt ttcccttttg
     7021 gaatgctaaa ggtcaggtca tctaaaacaa gtttttcctc ataagcaaag gaaaggtttt
     7081 gcacctgaat atgactgctg ttcttactca tataatatca gacctcctta gcaggtaaat
     7141 aaagtaaggc ccgcctacaa tactcattat aatcgcaggg ctaatttcca atgggtaagc
     7201 aagacttctt cctaaagtat cggctagcaa aaagacaaag gctccaagca aagctgaaaa
     7261 aggaattaag atttggtgct tacttccaac cagcaagcgg ctcatatgag ggacaatcaa
     7321 gcctaggaaa gaaaccactc caacgatcga agtggctacc gaagccaata aaacagcaac
     7381 caaggaaatt cccaaccgta acgctgtagc atcgattcct aaatgacgaa tcacctgatc
     7441 atctaaaaga agaaggttac aggtttttga caataacaaa gctagtaaca agccaatgaa
     7501 tgtataaggg aataaaaccc ctacatcagc ccatgttttt tgactaatat gacctgctaa
     7561 aagagcattg accatgggac tagctgaggt gaaaaaactg gttaaagcag ttgataaccc
     7621 cataaataaa gcattaatag caatccctgt tagaataagc cgaataggat taagcccttt
     7681 cttccatgct aaaccataaa tcagcaggaa agacactccg cctcctaaaa aggatacgat
     7741 tggaccatac agcagcaact gcggtagaag taaactagag actaaaacca taaaactagc
     7801 accactacaa atccctataa tagcagggtc tgaaatagga tttttcaaca ctgcttgaaa
     7861 aagaactccc gaaacagcta tccctgctcc tgctaataaa gcaatgacaa tccttggaaa
     7921 acgcaaatca taaatcaaag ccacttgggg gtcataagcc acaaacagtc ccttgacaat
     7981 tgccccataa gaaaccgcca aaccaccaag ggataaggca ataaccgcca aaaataggag
     8041 gctgagaatt aaaagaacta gataaagacc acttctgaaa cgttttttag ccagtaggga
     8101 ttgatccctt tgtaaatctg atgctgtcat gttacggatg atctcccacg tggtcaaaaa
     8161 gctgtgttaa ggtgtccaag gcttctgggt agttcaattt agcactcatg ccaaacaggg
     8221 tattgtccaa atcatagact ttcccttcct tgactgccgt aaaatgtttc caaatatcat
     8281 tttcagcaaa ttctttgtca aacatcactt ttaccttgtc tggaatggcg tgagctgttc
     8341 gtaaaatcaa gtcaggctcc ttagccagca tgtcttcagg attaactgat agaaattctt
     8401 tctcatctga ctgataaaca ttctcaccac ctgccaaatc caaaagattc cctacataag
     8461 attggttcgt cgccaccaaa taactacctg gcaagcccat aagaataagc actttaggct
     8521 ttttcttccc cttacgttta gcttggaaag cacgataata gtcctggtat tgctggcgca
     8581 attcttttgc ttcttgttga cgttggaaaa ggttccctaa atcatcaatg gactggtaca
     8641 tgccctcaac acttcgtaag ttcaaaaaac catactcagt atccaatttt tgatacttgg
     8701 gttccaaatc ttcttgtaaa gaattgggac tcaaaatcca agttggtttc aaagaagcaa
     8761 tcaactctat atcaggattc atgggtaaac ccacacgctt aacagcatca tagcgtttag
     8821 gaagggtata taatttacta tcacaaaccc caacgaggtc taaatttaaa cggtcacaga
     8881 tatcaaccac agcaaccgaa gtggctacaa ttctctgctg ttcagtctct ttagccgttt
     8941 tagagtgctg attcacacaa gctaccaaaa agaagaccat taaggctaga ccaattcctt
     9001 tacatcgttt tatcattttt tttcctcttt ttccaaaccc atacagcaaa ccctcctaac
     9061 atcagaaaaa aagttggaaa gtagtaaatc atgattgata agtctttttt agaccgaaac
     9121 ttatcttctt tttttaactc taaagaagtc gttaagccca tcttagaagg aagaattttt
     9181 ttatctgata gactcttatt atttgatgag ttggctttag gcttattctg agttatcata
     9241 gcacctgtgt gagactcatc ttgactttct cctagttttg tatcaacagg cttttgactg
     9301 tcttttactt cttgattttg agaattgtct gtttcagtaa caagctgggc caacatatta
     9361 cctgaatact tttgtattaa ctctgaagct gacaggtaaa agaccacttc tctgcccata
     9421 ggttcaacaa acatgcttcc tcgaatgata ctatttacag taggcaaact aatagcaata
     9481 tctagtgttg tcccattggt atcagtacct ttttgggtga tattgtaatc cactgcctga
     9541 aagcttccag ttcctccagg ttgtatccag aactgataat tgcccgaata atcagctaag
     9601 ctcatacgaa aagtaagaac gatctttcca gcatcagata cttctagcat ggcatcacta
     9661 tagacagtcc cttcaaccat accttgacca atatcaaagg aatgctcccc tccgctatct
     9721 tctatctgcc cagatatcgg atgacgataa tttctctgaa taatacatcc ataaatttga
     9781 cctttatctg catacacaac acctgtcatt gtcgataaac ttatcatgaa cactactatt
     9841 acctgcaaca attgctttat tactaccttt gtcatatgct cctcttgtct atcttttctt
     9901 aaacatctta tttcattttt taaatgaatc ccattttata acatctccaa atgtgaaaaa
     9961 cacacctatc ttgcgactac ttcaacctgt atcaggagtc ctgtcggaaa taggtatgtt
    10021 tttagctctt gctgactagc ttttttgtta tttcacttgg tactaagttt cttatttaaa
    10081 taatgtcttt cgaccaataa tgaatcccag agctacgatc ataaagctag atagacctgc
    10141 aattaagtga taacgagagc tactttcttc accatttgat tgttgattgg aacgatgttt
    10201 cttatcagat tttttagtct tagtatcttt tgccgtttta ccttcaattg atgtttgatg
    10261 atcacgcact aactgcttca atttctctgc cttatcagta tcatctgtct tagaagtttc
    10321 ttgagttgat tggccggttg cttttttagc caggagatca gttaagttag caataccagt
    10381 agctgctgaa ggaggacttg taggtgtttt ttcttgttga cgattttgag gtcctttcaa
    10441 ctgatctgtt gaagttagat ggctattttc ttgtggatta ttagtttctt tgacaacctt
    10501 tgccttctct gttgtaaggc ttgcccagtc taaacgcata aaaacggttt gaaggccaga
    10561 cccttttgaa atggcatcca tgataggcac aaagacctgt aatggcacaa gtccgtcttt
    10621 cttcgcttgt tcaatcaatt tcatacgcag cacttttgga tagttttttc cataacgatc
    10681 tgccaatggt aggccagtta catccgtgaa ataagacaca acctctgcct tttttgttcg
    10741 accaactggt ttaccagcta aatctctttg gtaaccatct acgaaatagc tcaattcacc
    10801 aaggtagcct aacattttac caacctttat acctctaaac tcaacttcta aataggaaac
    10861 acctttttta actaccaaag tcactcgatg attaatagcc ttgtttgaca tggactcaga
    10921 tgctaagtca gtttttaaca tgctggcatt taaatagtaa ataccatcac gcaattgttt
    10981 ttgatttaat gctctaaggc cgttctcaac taaatctttc tgatgtgaat gtggtccttt
    11041 cgccacaagg atatcattag atgtcaaata gatatgttct ttttgagtag caaatttctt
    11101 tgttattaaa gctttgcgaa tccgatatgt acctggatct tttaaagaaa actctcccgt
    11161 taagtcatct ttcggatcat ttgagagaag cttttcgtca accgtcacat actgtccatt
    11221 ggaagatttt ttttgcaact gaatcacaat actccaatcg acttgcatat cattaagtaa
    11281 agaaacagta tctgaacctt tttcttgtaa caattgttga tattcttcaa tagacactag
    11341 ttctttatca atggcagaat ttgttttttg ctcccaataa tacaaatcta aaagactgaa
    11401 agcttgatga tattttattt tttcgccatc taaagaagct tcaagttttg caatatgttt
    11461 ttgtggagtc agtttgtgtt gtccgataag agctcttact tttggactca cactttttat
    11521 ctgattgaac ttcacatcta aattaagcgt ttcaacatct ggaaatactg attcttcaac
    11581 ctgagtaagc aagttattga aaaaatcaag ttttgttaat tggttattct gtttaaatat
    11641 accatttgga agatttgaaa tttcattagt tgataaatcg agtacagata atttaggaag
    11701 atttttaaat gaaatattat caatacgagt aatatgattt ctacttgcca caatagtgct
    11761 taaggcgcgc aatttttcta ttgtttttgg cagtagtgct aaattatttt gagataagtc
    11821 gataaaatta agtgatgtta gaggctctag cgctttttca ggtatctcct caagactatt
    11881 ttctgcgaaa cctaatgaag tcagacgaga caattcttca aatgggttat cacaaagtcg
    11941 gccaatacga ttcttggaaa gatcaagttg ctccaggtga tgaagcgact ggaatagtcc
    12001 tccttcaaga gagtgaataa agttacttga taagtagagt tctcttaatt gagcattgct
    12061 tttaaatgta tctttgtcta gataagttaa atgattactt cttaagttaa gaaatcgaag
    12121 ttgcttcatt tgactaaata actcttttgg aatttccgta atttggtttc catccagatg
    12181 tagttctgta atatttttag catgtttcaa taaagaaata tctgttaact cgtaatggtc
    12241 tagacgcaac tcacctcgaa tctgttctaa ttccgttttt gataatttac catcgccgtc
    12301 taaatctttg ccaagtttaa ctaaaacttt ttcaagacgt tcagtcccaa cccctttttc
    12361 tttatcatca gtttctacct gataaccact ccaacctttg gtaacggtct tactaaaggt
    12421 catgtcaagt ttggtgtatt tgtcatagtg gtttaaatca ctttcttgac ctcccatggc
    12481 tgataccaaa acagcacctt tgtgtaattt atccaaatca tcaataggca tggtaaattc
    12541 gcttcgaata tagctaccat tgatatcttt ttggccaact tgtttacgca ctgctgctgg
    12601 gtaggtccct ttgctttcaa tagaaaagtc aatcaaaaat tgtcccaaag cagtattaag
    12661 catggaaatt tccattgtac catctgcttt aaccactaat ttggctcttt tatcaaaagc
    12721 accttgaagc atggaggact cttctgagtt ttctttatta gctttaaagt ttagcgtata
    12781 ggttccttct gtgagattgt taatagaaac ctcacctgct tgataagact cgatggtatc
    12841 ttttaacagt cgatgagtat cggcgagtag cttactatct ttgatttctt tgtaagaatc
    12901 cttgatgact tgaagtttct cagccagatc tttgaccgcg tctttgttgc tttctttagc
    12961 aataatcgca tccgcttctt tgatagcttt ttccaaagtg gtgacatcca actttgttga
    13021 ttgtctgtct tcagcagtca catgttgttt ttgtttggca gatacaaaat ggatttgacc
    13081 atctttcttg gtaacggtaa tgataacgtc ttcaaatcct tttgaggata tcacgattgt
    13141 attttctcca tctttgaaag cttttgttcc aagatgaagc cctgtcatat tcgagaccca
    13201 gccattagta tcttttacag aatctgaaat aagatcttta taaactgtac cattgactgt
    13261 aacctttgtg atttgctgag tcaatgcttt tatctgtttg ttatcagaat aacgatcaat
    13321 ttctggaaat aaaattgcac catcttcttg actcaatttt gttattaatg acaagttagc
    13381 agtatttttc ccagatttaa caactttttg tgtgccttgg ttttgtggca ctggtttatc
    13441 actgagcaca tcttttttta atgaatcctg atgatctgtt gttcctaatt tttgagctga
    13501 aataaaggtc actttatcgc cctttttaat aaaggtaatt aggatatcac catctttttt
    13561 attaacaata gtcaattcat gcttaccgtc tttaataagg ttagcattga tcttaatccc
    13621 ttggtagtta atatcaaagg ttcgctcgcc ttccaggctt ttttcataga catcattatc
    13681 aacaataacc ttttcaatat gtttataata ctcttttcca gctgattgac tagcgtaagg
    13741 gcctatctct tcaactaaaa gtcgattatc ctgagttaaa gctactgttg tcacgagttg
    13801 tgactgaact aaaggctctt gtgattttac agtttgagta gcctgaatga gaacaagggt
    13861 agatattgcc acaaacgcac atttgctgat ttttttcatt ttcaatcaac tttctaaaaa
    13921 tttttatgaa taagctaact tttttattaa ctgaacttaa tctatcactt tgtgattatt
    13981 ttgtcaagat tgtttccaat ggtccaattg tctgttaaaa agctccacta tgtgagccaa
    14041 gacactagca ataagcctga attgataatg aggatatctc tgataaaaga attcctctac
    14101 aacaatatta ctgagataaa gctagcctta ggatagttat tactagggct gattcaatca
    14161 ttacttcatt cacaaaacat gtaacacgtt ataagctcca taattgtgtt aggagacaac
    14221 atgagatgtc actactgcat attcatcaca ataaaaaaag acaatagcct taatcgtgct
    14281 attgtctcaa aatcatttat ttacttgaaa ctttgtcgtg ttacaccaac agtttaaaag
    14341 gcctaactcc cgctaagaaa attaagccgt aagaaaagtc attagttatt agtttttact
    14401 cttgccaata acatatgtta gctgttcagc ctgctgagct gtaaaggtac gataagaaat
    14461 cttacctaag ccgatacagt tagactctga aataagaata gacccatctt ttctaacatc
    14521 ttctacaatt gatacgtggc cataagtgcc atcagcgcct gcttgcccgg gtgcaaatga
    14581 aatagcataa cctacttttg gagtctttga cagggcatac cctgctttat acttccaatc
    14641 tccaccattt cccataaaag gatcaaattg gtaacccaat tctttggcgc gattatacac
    14701 ataccaagtg cattgtcccc aagaataact cgaagcttga taacccaaga catcataact
    14761 cttagagagc ttataacctg ctggcacaga tgcaaaacta gctgatgact gcactttcaa
    14821 ttcggctgga atggctggtg tgccaccatg gtcatcaatc cacttatcca ggtcttccat
    14881 aatttgtgcg cgacgtttac cactaccagt agtatcagta aaataaacac ccccgtcact
    14941 tgcaaagttt aattgacctc gtgaaaactt agccgctttt aaatcttgaa gtgcaaaatt
    15001 gctatttttg tttttaataa tggtatcttg tgtcattttg acaatagcac tatcatcatt
    15061 aaacttactt gcttgagtcg gatctagatc aaaagctgca tagccaaaca tattagctcc
    15121 aagcaaagtc gcaatccctt gagttcctaa agaactctca gacatcgcaa tagctacgat
    15181 agctcgaaca tccagcccac ttttcttttc ccattgcaat agcttttcac cattaatacg
    15241 tgtgctgtca taggcaatgc ctgttgattt taagtaacta tcaatctggt cagctttaat
    15301 accataacgg cgagataaaa gatggtgggt ataggcattt ttaccactcc aatgctctac
    15361 atatgccgcg tatgtgttga cagaagggat atctatctca ctcaaatcta aatctgatac
    15421 gtaaggtgac aacgactgaa taggagttct tacctctggc aactgtctcg acaatgaaga
    15481 agcaggtgcc gtatcaactt tagatggtga tggtttgggc tgatcagtcg gttttggctg
    15541 gtcagttggt ttcgaatgat cagttggttt cgaatggtca gttgatttat catctacctg
    15601 gttttctgct tctgccttgt tgtccgaaga acctacagca gactgactag aatcgcttct
    15661 ttttttatca tccgcgttaa caagactatt agagtttgtt tctgcagaag catcaagggg
    15721 accttggtca gttttatctg atgttgtagg tgctgcattt ttattatttt cctcttttga
    15781 agaagaatca ctggtttcat ttgtagttgc tttatgcgtc tcaagcatta catcttgagc
    15841 caacactgtc atgctttctg ctgtcggggc taagagactt agtagcatgg caactcttaa
    15901 tagtttgttt ttattcatat attaattagt cacttctttc taatgtcctc actttattat
    15961 aacattaaag ataagcacaa tgcaattatt tttacgtgat tttcttaata tatccgagga
    16021 atacggtcac ttaaaaggca tagaacttca taattgattg tattacggta atttgcgata
    16081 tctgttgtag aaatattttt ttgctgattg ctgccaatca aagtgacttt tgttcctaaa
    16141 ggatatgctt tgggaagtcg tatggtcagt tgatccatcg atacacgccc tataatttca
    16201 cagaattgtc catcaactaa caccgaaaag ccttgcatgt tcctggtcca gccatctgca
    16261 taaccgattg ggacggtccc tacatattca gactttttgg cagtataagt agctccataa
    16321 ccgactgtat caccagctga aatcatcttg acatgcacta agctagattc tagagataaa
    16381 gcctcttgca gtggaaatgg taagcttaga tcagaaccac ttggattcaa accataactg
    16441 acaattccta aacgaacagc attaaaaatg gtatcactat gccagatact tgtggctgaa
    16501 ttactagcat gtactaaacg aggcttatcc tcaagtccag ctatcagctt tttaaaaaac
    16561 tgtaactgct ggttaaattt agtatcatct gcctcatcag ctgtggcaaa atgagtaaaa
    16621 ataccttcta catcagcacc caactctttt agactatcaa tcaataaatt gacttctttt
    16681 gaagaacgaa gcccgatccg ccccatacca gaatcaactt ttacatgaac ttttaagcct
    16741 tgacattctt ttttctccag tctagctaaa gctatccagt ctaaactagc gattgtaaca
    16801 gtaatagcat tagcaactgc taactctaat tcatttggca ggaggacccc aagaattaaa
    16861 atctctttat caatacctgc ttgacgtaat tgcaaagcct catcaagatt tgacacacag
    16921 tacccatcca cttgaggtag gagtgctttt gacacctgga cagcaccatg accataagca
    16981 tcagccttga caactgcata cgtttttacc cctagtggaa tatgcttttg aacactggca
    17041 acattctcct taatagcttg taaattgacc cttgcaactg ttgggcgatg gaaacttgaa
    17101 atcatttttt atcctccaaa ataacactag cctgtacata attgccacta tgtgagatgc
    17161 taataaaact atttccttta aacggagatt ttgtcagaat gggacatcct ctaacatcat
    17221 ttaaaatctc aatatcttga aaggttaacc gcccaatacc tgtccctata gccttagcaa
    17281 aagcttcctt acctgaccag cgtcctgcca aatagctgag tcgacgtttg taaggaaaac
    17341 tctcaaaaat agccaattcc tgctcagtta aaattttttg agcaaagcga ggatttcttt
    17401 gataaacttt ttcaatagct gaaatctctt gtaagtctat tccatgtcca acaatcatta
    17461 ctttccacca aaccttattt catcttaatg ttagaagatt taaacagtga gaagctgaga
    17521 attattctca gcttctttct atatacctaa ctttgtcctg ttgtgtctcg ttcttatgag
    17581 aaggatttac gaccgtgaca atttttgaat tttttgcctg aaccacatgg acaagcttcg
    17641 ttacgctcca ctttaggtaa atcatcggtg ttagctgact gctgcgattg gatgttttga
    17701 ggtgctgctg ttgtggcacg ttgacttgca cgttcacgtt cttgttcgtg aatttgtgct
    17761 ttcatcatgg tacgtgttac atcaaactcg atggcaccaa tcatatcttg gaacatcttg
    17821 aaaccttctg cttggtattc caccacaggg ttattttgag catacccccg aagcccaaca
    17881 gcattacgaa gttgatctaa ggcatcaatg tgttcagtcc atttgttatc cacaatcatc
    17941 aaaatgagta ctttttggaa ttcaataata gcttcttgat cacgaagttt tgatagttgc
    18001 tggtcataga tagcaagcgc tctttgataa agcttctctt tgatttgatc atcttttaaa
    18061 ccacgtaact ctttagcact aatagactct tctggtacca gcgaggtcct agcaaaagtt
    18121 acaatagcat ctatagcatc tttacgattg ctacgtgcat gagcatctac agcacggtcg
    18181 atagtacgct tgatcatagc tttaatttct gggccaagat ctcgattagc agttatgaca
    18241 tcacgacggt tagcatagat aatttcacgc tgttcacgca tcacatcatc gtattgtaag
    18301 acctgtttac gggtatcgta gttatttcct tcaacccgtt tttgagcaga ttccacttga
    18361 cgccctaaca tacctgattt aataacagtg tcttcttcat ctaatttcat ccgatccaaa
    18421 aaggctttta tacgatcaga gccgaaacgt ctcatcaaat catcttctaa tgaaagatag
    18481 aattgggatt cacctggatc tccttgacga cctgaacgcc cacgaagctg gttatcgata
    18541 cgacgacttt catgacgctc tgtaccaatg acacataacc caccaagttc acgaacacct
    18601 tcaccaagct tgatatcagt accacgacca gccatgttag ttgcgattgt aacagcacca
    18661 cgttgcccag cattcataat gatttgagct tctttaaagt gatttttagc attcaaaacc
    18721 tcatgaggaa tccctgcttc aaccagcttg cgtgaaatca aatcactagt ttcaacagca
    18781 accgtaccta caaggatagg ttgtcctttt gcatgacgcg ttttaacgtc ttccacaact
    18841 gctctaaact tcgattctaa ggtcgggtaa agcaaatcag tgtgatcaat acgggcaatt
    18901 ggacgattgg ttggaattgg aataatacgc atattataaa cttcgcgaaa ttcttcttcc
    18961 tcggtcttcg cagtaccagt catacctgcc aattttttgt acatacgaaa catattttgg
    19021 taagtgattg aggcgctggt ttttgattct tcctgaatac gaacgccttc ttttgcctca
    19081 atagcttggt gcaagccgtc tgagaagcga cgcccttcca tcgtacgacc tgtaaattgg
    19141 tcaacaataa gaatctcacc atcttcgctg accacataat cgatatctaa caacataata
    19201 tagtttgcac gaagggcgtt gtctataaag tgagtcaagg ctacattttc aatatcgtaa
    19261 agattgctca agttaaaata actctcagct ttatcaatac ctgaatcgct taatccaatc
    19321 gttttagtgg gcacatcaat aacataatcc actgacgtca gagtttttac aaacatgtca
    19381 gcacgaatat acaattgatt ggtttcagag ctaacagcac cagaaacaat taaaggtgtc
    19441 cttgcctcgt caattaagac tgagtccact tcatcaacca aggcaaaatt gagtgggcgt
    19501 tgtaccatgt cttcctggcg aacaaccatg ttgtcacgaa ggtaatcaaa accgacttca
    19561 gagttggttg aataagtaat atcacaatta taagcttcac gtttttcagc aggagactta
    19621 gcagctaggt tgatcccaac tgaaagacct aaccagctat acacttcacc catttcggta
    19681 gcgtcacgcg ttgaaagata ctcattaaca gtaataacgt gaacaccctc accagcaata
    19741 gcattgagat aaactggcat ggtggctgta agagtttttc cttcaccagt acgcatttca
    19801 gggacatccc cgttatgaag aacaattcct cccataattt gaacacgata agggaacaag
    19861 cccaacacac gttttgctgc ttcacgaaca actgcgaaag cttcaggtaa tagttgttcg
    19921 agggtctcac ctttttgata gcgttcttta aattcaagtg tttttccttg tagatctcta
    19981 tccgacaatg acgccatttg gtctgcgtag gattctactt tttttgcaat tttttctaat
    20041 tttcttagtt cgcctttgtc attttcgata actttgcgta gaatattggc cattctcttt
    20101 cctttcgatc atctaatcat ttcattttaa cataaatatg caggcagttc aagaaaggtt
    20161 ggtaaagtta
//
LOCUS       DAUCLR010000028        19625 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.028, whole genome shotgun sequence.
ACCESSION   DAUCLR010000028 DAUCLR010000000
VERSION     DAUCLR010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19625
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.028"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(22..696)
                     /locus_tag="VGT87_000851"
     CDS             complement(22..696)
                     /locus_tag="VGT87_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEQ3813724.1"
                     /translation="MLKRKTVGVLGLGIFGRTVARELSNFDQDVIAIDIRESHVKEVA
                     DLVTKAAVGDITDKEFLLAVGVEHCDTVVIASGNNLESSVLAVMHCKKLGVPTIIAKA
                     KNKIFEEVLYGIGATKVITPERDSGKRVASNLLRRHIESIIYLEHGISMIEFVIPKSW
                     EGQSLSELDVRRKYELNVIGMRQKEVKTLDTNVKPFEPLEPNTIIVAIANDHTFEKFD
                     YLGYLK"
     gene            complement(706..2097)
                     /locus_tag="VGT87_000852"
     CDS             complement(706..2097)
                     /locus_tag="VGT87_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEQ3813725.1"
                     /translation="MSGGNMKRSFIKSLSVTQRLTFSFAIVILIGTLLLSMPFTHYQN
                     GPNTVYLDHFFNVVSMVCVTGLSVVPVAEVYNGIGQTIAMALMQIGGLGLVTLIAVST
                     FALKRKMRLSDQTLLQSALNRGDSKDLKHYLFFAYKVTFGLEAFAAIVIMIDFIPRFG
                     WKNGIFNSIFLAVSAFCNAGFDNLGSSSLKDFMLNPTLNVIITFLIISGGLGFAVWVD
                     LGVAFKKYFFERPHCYGATFRKLSNQSRLVLQTTAVILFLGTFLTWFLEKDNSKTIAN
                     FSLHQQLMVSFFQTVTMRTAGFATISYNDTLAPTNILYMIQMVIGGAPGGTAGGIKVT
                     TAAITFLLFKAELSGQSEVTFRNRIIANKTIKQTMTVLIFFFAVLMIGFILLLSVEPH
                     IAPIPLLFESISAIATVGVSMDLTPQLSTAGRLIVIVLMFVGRVGPITVLISLIQRKE
                     KTIQYATTDILVG"
     gene            complement(2167..2880)
                     /gene="rsmG"
                     /locus_tag="VGT87_000853"
     CDS             complement(2167..2880)
                     /gene="rsmG"
                     /locus_tag="VGT87_000853"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEQ3813726.1"
                     /translation="MTPQDFYRTLEEDGFSLSSKQKEQFDTYFKLLVEWNTKINLTAI
                     TEENEVYLKHFYDSIAPILQGFLANEPIKLLDIGAGAGFPSLPMKILFPNLEVTIIDS
                     LNKRISFLTLLAQELGLENVHFFHGRAEDFGQDKAFRGQFDVITARAVARMQVLSELT
                     IPFLKIGGKLIALKAQAADQELEEAKNALCLLFGKVIKNHSYQLPNGDSRFITIVEKK
                     KETPNKYPRKAGLPNKKPL"
     gene            3030..3587
                     /locus_tag="VGT87_000854"
     CDS             3030..3587
                     /locus_tag="VGT87_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEQ3813727.1"
                     /translation="MPTVLIILVVLGVLALWLMISYNSLVKSRMHTKEAWSQIDVQLK
                     RRNDLIPNLIETVKGYASYEQKTFEKITDLRARVANASTPQETMAASNELSKQVTSLF
                     AVAENYPDLKANENFLKLQEELTNTENKISYSRQLYNSTTSNYNLQLESFPSNIAGKL
                     FGFKPSEFLQTPEAEKEVPKVEFNF"
     gene            3634..4530
                     /gene="htpX"
                     /locus_tag="VGT87_000855"
     CDS             3634..4530
                     /gene="htpX"
                     /locus_tag="VGT87_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEQ3813728.1"
                     /translation="MLYQQISQNKQRTVVLLVVFFALLALIGASAGYLLLDNYAMGLV
                     LALVIGVIYATSMIFQSTSLVMSMNNAREVTEKEAPGFFHIVEDMAMVAQIPMPRVFI
                     IEDPSLNAFATGSSPQNAAVAATTGLLEVMNREELEGVIGHEISHIRNYDIRISTIAV
                     ALASAVTVISSIGGRMLWYGGGSRRQRDDGDDDVLRIITLLLSLLSLLLAPLVASLIQ
                     LAISRQREYLADASSVELTRNPQGMIKALEKLQLSQPMKHPVDDASAALYINEPRKKR
                     SFSSLFSTHPPIEERIERLKNM"
     gene            4764..5297
                     /locus_tag="VGT87_000856"
     CDS             4764..5297
                     /locus_tag="VGT87_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEQ3813729.1"
                     /translation="MLAISEIRKHPDGLSFDRLCDVKSMLLERDQQIIDIKAVKAVGN
                     VRYDKGLYLLDYQLSYEVILPSSRSMVPVCLSEVQHIQELFIEATDLADKKELVEDNL
                     VLVLDKDAINLEESIVDNILLAIPLQVLTEEEKKSKELPAGQNWAVLTEEDYQCLKEE
                     KQKENNPFASLQGLFDE"
     gene            5564..6250
                     /gene="covR"
                     /locus_tag="VGT87_000857"
                     /gene_synonym="csrR"
     CDS             5564..6250
                     /gene="covR"
                     /locus_tag="VGT87_000857"
                     /gene_synonym="csrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CovR"
                     /protein_id="HEQ3813730.1"
                     /translation="MTKKILIIEDEKNLARFVSLELQHEGYEVIVEVNGREGLETALE
                     KEFDLILLDLMLPEMDGFEVTRRLQTEKTTYIMMMTARDSIMDVVAGLDRGADDYIVK
                     PFAIEELLARIRAIFRRQDIESEKKVPSQGIYRDLVLNPQNRSVNRGDDEISLTKREY
                     DLLNILMTNMNRVMTREELLSNVWKYDEAVETNVVDVYIRYLRGKIDIPGKESYIQTV
                     RGMGYVIREK"
     gene            6256..7758
                     /gene="covS"
                     /locus_tag="VGT87_000858"
                     /gene_synonym="csrS"
     CDS             6256..7758
                     /gene="covS"
                     /locus_tag="VGT87_000858"
                     /gene_synonym="csrS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CovS"
                     /protein_id="HEQ3813731.1"
                     /translation="MENQKQKQKKYKNSLPKRLSNIFFVLFFCIFSAFTLIAYSSTNY
                     FLLKKEKQSVFQAVNIVRVRLSEVDSNFTLENLAEVLYKNDKTHLRIDDRKGSRVIRS
                     ERDITNTLDANQDIYVYNIDKQMIFTTDNEESSPGLHGPIGRVYHDHIEDQYRGFSMT
                     QKVYSNRTGKFVGYVQVFHDLGNYYVIRARLLFWLLVVELFGTSLAYLIILITTRRFL
                     KPLHNLHEVMRNISENPNNLNLRSDISSGDEIEELSVIFDNMLDKLETHTKLQSRFIS
                     DVSHELRTPVAIIKGHIGLLQRWGKDDSDILEESLTATAHEADRMAIMINDMLDMIRV
                     QGSFEGHQNDMTVLEDSIETVVGNFRVLREDFIFTWQSENPKTIARIYKNHFEQALMI
                     LIDNAVKYSRKEKKIAINLSVTGKQEAIVRVQDKGEGISKEDIEHIFERFYRTDKSRN
                     RTSTQAGLGIGLSILKQIVDGYHLQMKVESELNEGSVFILHIPLAQSKES"
     gene            7973..8467
                     /gene="nrdR"
                     /locus_tag="VGT87_000859"
     CDS             7973..8467
                     /gene="nrdR"
                     /locus_tag="VGT87_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEQ3813732.1"
                     /translation="MRCPKCNYHKSSVVDSRQAEDGNTIRRRRECEQCHTRFTTFERV
                     EELPLLVIKKDGTREQFSRDKILNGVVQSAQKRPVSSTDIENVISRIEQEVRTTYENE
                     VSSTAIGNLVMDELAELDEITYVRFASVYKSFKDVDEIEELLQQITNRVRGKKKRLNN
                     DETN"
     gene            8451..9626
                     /locus_tag="VGT87_000860"
     CDS             8451..9626
                     /locus_tag="VGT87_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEQ3813733.1"
                     /translation="MMKPIDTFTYLKRNKVTLDSVTLIQLYFPIIGSDAVSIYQYFIH
                     FFDDGLQRHKFSDILNHLQFGMKRFEDALAILTAMELVSVYQLSDTYLITLHQPLSRD
                     LFFQHPAYSRLLEQKIGEVAVSELQVTVPSQARNISKRFSDIFGVQENLTNVPQKPQK
                     NFDLSSFQQLMVRDGLQFEDNQKDIISLYSIAEQYDMTWFDTYQIAKATAVNGKIRPE
                     RLLAKKNQSMTKPSKENFSQAEQIILREAKQDSALVFLEKIKKARRATITKDERILLQ
                     TLAKMNFLDDVINVMVLYTFNKTKSANLQKSYVLKMANDFAYQKVSTAEEAIVVLRAF
                     TDRQSCRQSKVKTSQSNVPKWSNPDYQETTSQEEQAKLDQFKQAALKRLENLGKGGD"
     gene            9627..10529
                     /gene="dnaI"
                     /locus_tag="VGT87_000861"
     CDS             9627..10529
                     /gene="dnaI"
                     /locus_tag="VGT87_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEQ3813734.1"
                     /translation="MEKIGETMAKLGQNTRVNSDQLIQTILADPEVASFISQHHLSQE
                     QINLSLSKFNQFLVERQKYQLKDPSYIAKGYQPILAMNEGYADVSYLETKELVEAQKQ
                     AAISERIQLVSLPKSYRHIHLSDIDVNNASRMEAFSAILDFVEQYPSAEQKGLYLYGD
                     MGIGKSYLLAAMAHELSEKKGVSTTLLHFPSFAIDVKNAISNGSVKEEIDAVKNVPVL
                     ILDDIGAEQATSWVRDEVLQVILQYRMLEELPTFFTSNYSFADLERKWATIKGSDETW
                     QAKRVMERVRYLAREFHLEGANRR"
     gene            10592..11902
                     /gene="der"
                     /locus_tag="VGT87_000862"
     CDS             10592..11902
                     /gene="der"
                     /locus_tag="VGT87_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEQ3813735.1"
                     /translation="MVLPTVAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRQFSLIDTGGIDDVDAPFMEQIKHQAQIAMEEADVIVFVVSGKEGVTDADEY
                     VSKILYRTNTPVILAVNKVDNPEMRNDIYDFYSLGLGDPYPVSSVHGIGTGDVLDAIV
                     ENLPVEEAEENDDIIRFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DADGQEFTMIDTAGMRKSGKIYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTIDKDNHTVAKWEADIRDQFQFLTYAPIIFVSALTKQ
                     RLNKLPDLIKRISESQNKRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVSVKP
                     PTFVVFVNEEELMHFSYLRFLENQIRAAFTFEGTPIHLIARKRK"
     gene            12109..15207
                     /locus_tag="VGT87_000863"
     CDS             12109..15207
                     /locus_tag="VGT87_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEQ3813736.1"
                     /translation="MMARLIPGRVRNEGIKLYEQGLVSFQDDNKGILQIEVETYQVQY
                     GADDEDITCQCDTFHMKHYCKHIAAVEYFLKNDQKGKLFLKQLTNQTKIKETTKKMTS
                     FGSLFLDSLAMNEDDSVKYRLSALGSRSPFSSDYWWSLKINRLPDDRSYVIRDIKGFL
                     QLIKKEGFYQIGKNYFEQLSWLQFDPSSQDLIEFLWRLASNTDKGDNENIFPNHARHL
                     RLPSGFFEEGIHYLTSLYDFTFEGPSQTYHHLFVRSLEAEAGLYEFKVEVHRKSIELQ
                     IAEKNVQYLFDNDYLLYQDTFYHLTLKQRKMVQAIRSLPIEADLAKHIHFDLDDHAKL
                     AASLSDFKQIGLVEAPKSFAIRDFEVTFQFDLLNRDEISCQLMFDYGNYQVSDKASLE
                     ALPFASHLKKEEKINRSLLAFGFSPQFYSKKRLTSAKELYTFFEETVPCFERLGNVAL
                     STAIQALQVKEMPKIAIRRNQGLLDISFDFSTIIENDIDQAVTALFQNNPYFVSQTGQ
                     LVVFDDETQKVSKSLQELRARQLKNGHLQLDGIRALQVSKLFEGMTSVHFSKELEELA
                     YHLQHPETFSIKPLPVKAQMRDYQRNGVQWLSMLNHYGFGGILADDMGLGKTLQTLAF
                     LASHLKSDSKVLILSPSSLIYNWFDECQKFTPQLDVVVSYGLKQIRDQIIEEGHQITI
                     TSYSSFRQDFETYQAFHYDYLILDEAQVIKNAQTKISHCLRAFNTANCFALSGTPIEN
                     KMLEIWSIFQIVLPGLLPTKKEFLKLTAEQVSRYIKPFVMRRKKEDVLPELPDLIETN
                     YSNEMTDEQKAIYLAQLRQMKDQIRNSSDVDISRQKIEILSGITRLRQICDTPSLFMD
                     YQGKSGKLDSLRILLTQIKENGHRALIFSQFRGMLDLAKQEMTALGLTSYQMTGSTPA
                     NERQEMTRAFNNGSKDAFLISLKAGGVGINLTGADTVILIDLWWNPAVEMQAISRAYR
                     IGQKENVEVYRLITRGTIEEKILELQESKRNLVTTVLDGNESRASMSIEEIKEILGLN
                     TH"
     gene            15450..16052
                     /locus_tag="VGT87_000864"
     CDS             15450..16052
                     /locus_tag="VGT87_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813737.1"
                     /translation="MVKNQFPLVADGIAISDPAKQMALYENEDLITNIRGYYQDKEYD
                     DIARNEEFTAKATSRQAPSSKRFCSNDEKHHYVKEARQKAKQDLKEKRQAYLAKEMAY
                     VPKQVSKKQQPADSSPSQKQATTEMSRFTKKLHQDNYILAELPKEYKEPKNLPQQGTT
                     KKNNYDFLKSSQIYNNKEMRQQREKTIAQELNLSRFEDLP"
     gene            16092..17420
                     /locus_tag="VGT87_000865"
     CDS             16092..17420
                     /locus_tag="VGT87_000865"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEQ3813738.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFSEDNITPDMELIVGNAFRENNKEVAYALRHQIPFKRYHDFLGDFMKSF
                     ISFAVAGAHGKTSTTGLLSHVLKNITDTSYLIGDGTGRGSANAQYFVFESDEYERHFM
                     PYHPEYSIITNIDFDHPDYFTGIADVRNAFNDYAKQVKKALFVYGEDDELKKIEAPAP
                     IYYYGFEEGNDFIAYDITRTTNGSDFKVKHQGEVIGQFHVPAYGKHNILNATAVIANL
                     FVAGIDMALVADHLKTFSGVKRRFTEKIINDTIIIDDFAHHPTEIVATIDAARQKYPS
                     KEIVAIFQPHTFTRTIALLEDFACALNEADSVYLAQIYGSAREVDKGEVKVEDLAAKI
                     IKPSQVVTVENVSPLLDHDNAVYVFMGAGDIQLYEHSFEELLANLTKNNQ"
     gene            17466..17948
                     /locus_tag="VGT87_000866"
     CDS             17466..17948
                     /locus_tag="VGT87_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ3813739.1"
                     /translation="MLIRQVQGSDLEVIATIESDNFSPQEATTRAVLEERIRLIPDTF
                     LVALIDQEIVGYIEGPVVTTPILEDSLFHGVTKNPKTGGYIAITSLSIAKHFQQQGVG
                     TALLAALKDLVVAQQRTGLILTCHDYLISYYEMNGFINQGISESQHGGTLWYQMIWKL
                     "
     gene            18060..>19625
                     /gene="mltG"
                     /locus_tag="VGT87_000867"
     CDS             18060..>19625
                     /gene="mltG"
                     /locus_tag="VGT87_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEQ3813740.1"
                     /translation="MALTDFKDKDQQDQQRSFKEQILAELEKANQIRKEKEEELFQKE
                     LEAKEAAKRTAQLYAEYKRQDAFQKESIAHNNKTAKHFQAIKGAVMTSEALKPTLLSE
                     KENSSLKTTNKRVVQANELQETASKESQVPLTIEKGHSVRRKLSKRQQTERAAKKIST
                     VLISSIIITLLAVTLAGAGYVYSALNPVDKNSDAFVQVEIPSGSGNKLIGQILQKKGL
                     IKNSTVFSFYTKFKNFTNFQSGYYNLQKSMSLEEIASALQEGGTAEPTKPSLGKILIP
                     EGYTIKQIAKAVEHNSKGKTKKAKTPFNEKDFLDLVTDEAFIQDMVKRYPKLLATIPT
                     KEKAIYRLEGYLFPATYNYYKETTMRELVEDMLAAMDATLVPYYDKIAASGKTVNEVL
                     TLASLVEKEGSTDDDRRQIASVFYNRLNSGMALQSNIAILYAMGKLGEKTTLAEDATI
                     DTTINSPYNIYTNTGLMPGPVASSGVSAIEATLNPASTDYLYFVANVHTGEVYYAKTF
                     EEHSANVEKYVNSQ"
BASE COUNT         6445 a         3348 c         3964 g         5868 t
ORIGIN      
        1 aacgccaagg atgttttttt attatttaag atagccaaga taatcaaatt tttcaaaggt
       61 gtggtcatta gcgatggcca caatgatagt attcggttct aaaggttcaa aaggtttgac
      121 attagtatct aaggttttaa cttctttttg acgcatgcca atcacattta actcatattt
      181 acgacgaaca tctagttcgg aaagagattg tccttcccag cttttaggaa tgacaaattc
      241 aatcatcgaa ataccatgtt ccaaatagat gatgctttca atatgacgtc ttaacagatt
      301 tgaagctaca cgttttccag aatcacgttc aggggtgatg actttagttg ctccgatgcc
      361 atatagaact tcttcaaaaa ttttattttt agccttggca ataatggttg gaacaccgag
      421 ttttttacag tgcatgactg caagaacaga agattccaag ttattccctg aagcgataac
      481 gacagtatca caatgttcaa cccctacagc taatagaaat tctttgtcag taatatctcc
      541 aacagcggct ttagtgacca agtcggccac ctctttgaca tgactttctc tgatatcaat
      601 agcaataacg tcttgatcaa agttacttaa ttctctggca acagtacggc caaaaatacc
      661 aaggccaaga acgccgacag ttttacgttt taacatgttt tctccttaac ccactagaat
      721 atcagtagtg gcatattgaa tggttttttc ttttcgttga atcagactaa tgaggacagt
      781 aattgggcca acgcggccga cgaacatcaa gacaatgaca ataagacgtc cagcagtaga
      841 caattgtggt gtcaaatcca tagatacccc aactgtcgcg atggcagaaa tagattcaaa
      901 taagagtggt ataggagcga tatgaggctc cacgctaagt aataaaataa aacctatcat
      961 aagaactgca aaaaagaaga taagaactgt catggtctgc ttaattgtct tatttgcgat
     1021 aattcggtta cgaaaggtaa cttctgattg ccctgaaagt tccgctttaa agagtaagaa
     1081 agtaatggca gcagtcgtca ctttaatccc accagctgtt ccaccaggag caccaccaat
     1141 aaccatttga atcatataaa gaatgttagt tggtgctagg gtatcgttat aagagattgt
     1201 tgcaaagcca gctgttctca tagtgactgt ttgaaaaaat gaaaccataa gttgctgatg
     1261 taaactaaaa ttggcaatgg ttttgctgtt atctttttct aaaaaccagg ttaagaaggt
     1321 tccaagaaaa agaataacag ctgtggtttg tagtactaga cgagactgat tggataattt
     1381 acgaaaagtt gccccataac aatgaggtct ttcaaaaaag tattttttaa aagctactcc
     1441 taaatcaacc caaaccgcaa aaccaagtcc accagagata atcaagaacg taatgataac
     1501 atttaatgta ggatttaaca taaagtcttt taaactagaa gatcctaaat tgtcaaaacc
     1561 agcgttacaa aatgccgaca cagctaaaaa aatactattg aagataccat ttttccaccc
     1621 gaagcggggg ataaagtcaa tcatgataac tatcgcagca aaggcttcta gaccaaaggt
     1681 tactttataa gcaaaaaaaa ggtagtgctt taaatctttg ctatcacctc gatttaacgc
     1741 tgattggagc aaggtttgat cgctcaatcg catttttcgc ttgagggcaa aagtactcac
     1801 agctattaac gtcaccaagc caagaccacc aatctgcatt agtgccatag caatggtttg
     1861 tccgataccg ttatacactt cagcgacggg aacaacagaa agtccagtca cacaaaccat
     1921 ggaaaccaca ttaaagaaat gatcaagata gacggtatta ggaccatttt gataatgggt
     1981 aaagggcatg gataacaaga gagtcccaat cagaatcaca atggcaaaac taaatgttaa
     2041 gcgctgtgtg actgaaagag atttgataaa agagcgtttc atgtttcctc cagacataat
     2101 atctcctact ataataacaa aaataagtta aaaaagctaa gtttcatctt agcctttggt
     2161 actattttat agaggttttt tgttaggcaa gccagctttt cttggatact tgttaggtgt
     2221 ttcctttttc ttctcaacaa tggtgatgaa gcgagaatct ccatttggca attggtagct
     2281 atggttttta atgactttac caaaaagaag acaaagagca ttcttagctt cttctaactc
     2341 ttggtcagca gcttgtgcct ttaaggcgat taattttcct ccaattttta aaaaggggat
     2401 ggttaattca gatagaacct gcatccgagc aaccgctctg gcagtaatga catcaaattg
     2461 acctcgaaag gccttgtcct gtccgaaatc ttctgctcta ccatgaaaaa aatggacatt
     2521 ttccaagccc aattcctgag caaggagtgt cagaaaagaa atccgcttat taagagaatc
     2581 aataatggta acctcaaggt ttgggaaaag aattttcatg ggtaggctag gaaaaccagc
     2641 tccagctcca atgtccaata atttgattgg ttcattagct aaaaagccct gtaaaatagg
     2701 agcgatggaa tcataaaaat gcttgaggta aacctcattt tcttctgtaa tagctgttaa
     2761 attaatttta gtgttccatt ccactagtaa tttgaagtag gtgtcaaatt gttctttttg
     2821 tttcgatgat aaggaaaaac catcttcctc tagtgttcgg taaaagtctt ggggtgtcat
     2881 ttgtatcctc tgtttttgat agttgttacc attttatcat aaaatagccc ataacaaaat
     2941 ccaataaatc atcagaaata acacctcatt acttgctatt agcctagaaa ttgctataat
     3001 tgagacacaa agattaaagg agacccacta tgccaacagt tttgattatc ttagtcgttt
     3061 taggagtgct tgcgctatgg ttaatgatta gttacaatag cctggttaaa tctcggatgc
     3121 acacaaaaga agcttggagt cagattgatg tgcaattaaa gcgtcgtaat gacttgattc
     3181 caaatcttat tgaaaccgta aagggatatg ctagctacga gcaaaagaca tttgaaaaaa
     3241 tcactgattt gcgtgcgcgt gttgcgaatg cctcaactcc tcaagaaacc atggcggctt
     3301 ctaacgaatt gagtaaacaa gtgaccagtt tgtttgccgt tgctgaaaat tacccagact
     3361 taaaagctaa cgaaaacttc ttgaaattac aagaagagtt gaccaatacg gaaaataaaa
     3421 tctcatattc tcgtcaactc tataattcaa caacgtctaa ttacaacctt caattagaat
     3481 ctttcccaag caatatcgct ggtaaattat ttggttttaa accaagtgaa ttcttacaaa
     3541 caccagaagc tgaaaaagaa gttccaaaag ttgaatttaa cttttaaata tgaaattatc
     3601 atctagtagg caatcataag agaaaggaga attatgcttt atcaacagat ttcacaaaat
     3661 aagcaacgaa cagttgtttt attagttgtt ttcttcgctc tcttagcact tattggagct
     3721 tcagcaggct atctgctgtt agataattat gccatgggct tggttcttgc tcttgtcatt
     3781 ggggtgattt atgctaccag tatgattttt caatccacca gtcttgtaat gagtatgaac
     3841 aatgctagag aagttacaga aaaagaggct ccaggcttct tccatattgt cgaggatatg
     3901 gctatggtgg cccagattcc gatgccgaga gtttttatta ttgaagatcc ttctttaaat
     3961 gcttttgcga caggatctag ccctcaaaat gctgctgttg cagcaacgac aggtttactt
     4021 gaagtcatga atcgtgaaga acttgaaggt gttatcggcc atgaaattag ccacatccga
     4081 aattatgata tccgtatttc gactattgct gtagctttgg cgagtgctgt taccgttatt
     4141 tctagtatcg gtgggcgtat gctatggtat ggtggcggtt ctcgcaggca aagagatgac
     4201 ggagatgatg atgttttaag aatcattaca ttacttttgt cgttattatc tttgctttta
     4261 gcgcccctag tagcaagttt gattcagttg gctatttctc gtcaaagaga atatctagct
     4321 gatgctagtt ctgttgaact aacaagaaat ccccaaggta tgattaaggc tcttgaaaaa
     4381 ttgcagttat ctcagccaat gaagcatcct gttgatgatg ctagtgcagc cttgtatatt
     4441 aatgagcctc gcaaaaaaag gagcttcagt tcattattca gcacccatcc tcctattgag
     4501 gagaggattg aaaggttaaa aaacatgtga gcgtttgggg tttttgctca agcctcactt
     4561 ttttatatct ttgggtagat aatctaaaat tgcaggaatt atcaaaagta tctgaacaaa
     4621 aagaaaaaaa tgagtgaaac aatggttaaa aatagaatga tttaaatctt aaaattaaaa
     4681 aacgtcagaa aataaatgca aaactattga caaatctttt agatagtagt aaaaataaat
     4741 gagagtttta ttaggagaag atgatgttag ctatttccga aatcagaaaa cacccagacg
     4801 gtctctcgtt tgatcgttta tgtgatgtca aaagtatgtt acttgagcgt gaccaacaga
     4861 ttattgacat aaaggcagtt aaagctgtcg gtaatgttcg ctatgataag gggctttatc
     4921 tgttagatta tcaattgagt tacgaagtga tattaccgtc tagccgttct atggttccgg
     4981 tctgtttaag tgaagtccaa cacattcaag aattatttat agaggcgaca gatttagctg
     5041 ataagaaaga gttggttgaa gacaatctgg tattagtttt agacaaagac gctattaacc
     5101 tcgaagaaag tattgtggat aatatcttac ttgctattcc gctacaggtc ttgacagagg
     5161 aagaaaagaa atcaaaagag ttgccagcag gtcaaaattg ggcagttttg acagaagaag
     5221 actaccagtg tctaaaagaa gaaaagcaaa aagaaaataa tccttttgct agcttgcaag
     5281 ggttgtttga tgaataaaac tgaatattaa agatgtctgt aatttttatt tgaaaaaaca
     5341 gctattcttt gatatactct ttagagaata tggttactta tctaaagtag aaggtatttt
     5401 gaccatagag ggcaaagaag gtaatgacta ttagatgctt catattttat tcgctagaag
     5461 actatttgac cattagaact gaaatagtct aggatatgag atggatttca tagttttctt
     5521 tctgggagaa aaagatagat taagaggata agggttggta taaatgacaa agaagatttt
     5581 aattattgaa gatgaaaaga atctggctag attcgtttct cttgagctgc aacatgaggg
     5641 ttatgaagtc attgttgagg tcaatggtcg tgaagggtta gaaactgctt tggaaaaaga
     5701 gtttgattta atcctgcttg acttaatgtt accagagatg gatggttttg aagtgacccg
     5761 tcgtttgcaa accgaaaaaa caacgtatat catgatgatg actgcgcgtg attctattat
     5821 ggatgtggtt gcaggtttag accgcggtgc agacgactat attgttaaac cgtttgccat
     5881 tgaagaacta cttgcccgta ttcgtgctat tttccgccgt caagatattg aatctgagaa
     5941 gaaagtgcct agtcaaggca tttatcgaga tctagtttta aatccacaaa accgttcagt
     6001 taatcgtggc gacgatgaga tttctctcac taaacgtgaa tatgatttgc ttaatatttt
     6061 gatgactaat atgaatcgtg tcatgacacg tgaagaatta ttgtcaaatg tttggaaata
     6121 tgatgaagcc gttgagacta atgttgtaga tgtctatatt cgttatctcc gcggcaaaat
     6181 tgacattcca ggcaaggaat cttatatcca aacagtgcgt ggcatgggat acgttattcg
     6241 tgagaaataa gtcatatgga aaatcagaaa caaaaacaga agaaatataa aaactcgtta
     6301 ccaaaacgac tatctaatat cttttttgtt ctttttttct gcattttctc tgcctttaca
     6361 ctgattgcct atagttcaac aaactatttc ttattgaaga aagaaaagca gtcagtcttt
     6421 caagctgtaa atattgttag agttcgtctt tctgaggtgg actctaattt tacattagag
     6481 aacttagcag aagttttgta caaaaacgat aaaacacatc tgagaattga tgacagaaag
     6541 ggcagtcgag tcattaggag tgagcgtgat atcacaaata ctctagatgc gaatcaagat
     6601 atttatgtct ataacattga taaacagatg atttttacca cagataacga agaatcatct
     6661 cctggcttgc atggtcctat cggtcgtgtg tatcacgacc atatcgaaga tcagtatcgt
     6721 gggttttcca tgacacaaaa ggtatattct aatcggactg gaaaatttgt gggctatgtt
     6781 caagtctttc atgatttagg caattattat gtcattagag caagactgct gttttggcta
     6841 ctagttgttg agttatttgg cacaagctta gcctatttaa tcatcttaat tactacgcgg
     6901 cgctttttga agcctttaca caatttacat gaagtgatgc gtaatatctc tgaaaatcct
     6961 aataatttaa acctgcgctc agatatttcg tcaggagatg aaatcgaaga attgtctgtt
     7021 atctttgaca atatgttaga caaattggag acacatacta agttgcaatc acgttttatt
     7081 agtgatgtca gccatgaatt acgaacaccg gttgcgatta ttaaagggca tattggtctc
     7141 ttacaacgtt ggggtaaaga tgacagtgat attcttgaag aaagcttgac agcaacagcg
     7201 catgaggctg accgtatggc aatcatgatt aatgatatgt tagacatgat tcgcgtacaa
     7261 ggctcatttg agggacatca aaatgatatg acagttttgg aagattctat tgaaactgtt
     7321 gttggtaatt ttagagtttt aagagaagat tttatcttta catggcagtc agaaaatcca
     7381 aaaacgatag cccgtattta taaaaatcat tttgagcagg ctttgatgat tctgattgac
     7441 aatgctgtga agtattcccg taaagaaaag aaaatcgcga ttaacctttc agtgactggc
     7501 aaacaagaag ctattgttag agttcaagat aaaggcgaag gaatttctaa agaagatatt
     7561 gaacatatct ttgaacgctt ttatagaacg gataaatcac gtaatcgaac aagtacccag
     7621 gctggattag ggattggctt gtctattctc aagcaaattg tagatgggta tcatttacag
     7681 atgaaggttg aaagtgaatt aaatgaaggt tcagtgttta tcttacatat tcctttggcc
     7741 cagtctaaag agagttagag tagcttaagg cattgttaat gtgacaggta tagtatgtaa
     7801 tcattagata atagtagcag tcttttgcca caactggtta gaagctgatg ctattttttt
     7861 agcaatgatg gatgacatga aagaaatact gtcaatcgcg atgccttttg ttatccttga
     7921 gaatatggta aaatagtgta ttagattaat ttgatagaat tggggtgttt gcgtgcgttg
     7981 tccaaaatgt aattaccata aatcgagtgt tgttgacagt agacaagctg aagacggcaa
     8041 caccattcgt cgtcgaagag aatgtgaaca gtgtcatact cgctttacta cctttgagcg
     8101 tgttgaagaa ctccctttat tagtcatcaa aaaagatggc acacgagagc aattttctag
     8161 agataaaata ttaaatgggg ttgtccaaag tgcgcaaaaa cgacctgtct cgagcacaga
     8221 tattgaaaat gtcatttctc gtattgaaca agaagttcga acaacctacg aaaatgaagt
     8281 gtcaagtact gctattggta atttagtgat ggatgagttg gccgaactcg atgaaatcac
     8341 atatgtacgc tttgctagtg tctacaagag cttcaaggac gttgatgaaa tcgaagaact
     8401 tttgcaacaa attacgaatc gcgtacgcgg aaagaaaaaa cgtttaaata atgatgaaac
     8461 caattgatac ttttacatat ctaaaacgca acaaagtaac tcttgattcg gtgactttaa
     8521 tacagttgta ctttccaatt attggaagtg acgcagtctc tatctatcaa tactttattc
     8581 acttttttga cgatggttta caacgtcata aattcagcga tattttaaat cacctacagt
     8641 ttggtatgaa acgttttgaa gatgctcttg ccatattgac agcaatggaa ttagtgagtg
     8701 tgtatcagct atcagataca tatctcatta cgttgcacca gccgcttagc cgtgacttgt
     8761 tttttcaaca tccagcttat tctcgattat tagaacaaaa gattggagag gtagctgtct
     8821 ctgagttgca ggtaactgtc ccaagtcaag caagaaatat ttctaagcga ttttcggata
     8881 tttttggggt gcaagaaaat ttgacgaacg tacctcaaaa gccacaaaag aattttgacc
     8941 tttcaagttt ccagcaatta atggttcgtg atgggttaca atttgaagat aatcaaaaag
     9001 atatcattag cctttacagt attgctgaac agtatgatat gacgtggttt gatacttatc
     9061 aaatagctaa ggcgacggct gttaatggaa aaattcgacc tgaacgctta ttggctaaaa
     9121 aaaatcaaag catgacaaaa ccaagcaaag aaaatttttc acaggctgag caaattattt
     9181 tgcgagaagc aaagcaagac agtgctttag tctttttgga aaaaattaaa aaggctagac
     9241 gtgcaacgat tactaaggat gaacgcattt tattgcaaac attggccaaa atgaattttc
     9301 ttgatgatgt tatcaatgtc atggtgcttt atacctttaa taagacaaaa tctgctaatt
     9361 tacagaagag ttatgtctta aaaatggcca atgattttgc ctaccaaaag gtgtcaacgg
     9421 ctgaagaagc tatagtggtt ttgcgagcct tcacggatcg tcaaagttgt cggcaatcaa
     9481 aagtaaaaac aagtcaaagc aatgtgccta agtggagcaa tcctgattac caagagacaa
     9541 caagtcaaga agagcaggcg aaactagatc agttcaaaca ggctgctttg aagaggttag
     9601 aaaacctagg aaaagggggt gactagatgg aaaagattgg agaaaccatg gcgaaattgg
     9661 gtcaaaacac tcgtgttaat agcgaccagt tgattcaaac cattttagcg gatcctgaag
     9721 tagcaagctt catcagtcaa catcacttat ctcaagagca aattaacctt agcttatcta
     9781 agttcaatca atttttggtg gaacgccaga agtatcaact caaagatccc tcttatattg
     9841 ctaagggcta tcagcctatt ctagctatga atgagggcta cgctgatgtg tcttatttgg
     9901 aaaccaaaga attggtagaa gctcaaaaac aggcagctat ctctgaacgg attcaactgg
     9961 ttagtttgcc aaaatcctat cgccatattc atttatcaga tattgatgtt aataatgcga
    10021 gccgtatgga agccttctct gctattttag attttgtgga acaataccca tcagcagagc
    10081 aaaaaggctt atatttgtac ggagatatgg gaattggcaa gtcttacttg ttggctgcta
    10141 tggcccatga attgtctgag aaaaaaggtg tgtcaacgac ccttctacat tttccgagct
    10201 ttgccattga tgtaaaaaat gccatttcaa acggctctgt taaagaagaa atcgatgcgg
    10261 tcaaaaatgt accagtgctc attttagatg atattggtgc agagcaagcc acttcttggg
    10321 tccgtgatga ggtgttgcag gtgattttac aataccgtat gttagaggaa ttgccaacct
    10381 tctttacctc taattacagc tttgcggatt tggagcgaaa atgggcaacc atcaaaggaa
    10441 gtgacgaaac ttggcaggcc aaacgtgtca tggaacgcgt gcgttatttg gctagagagt
    10501 ttcatttaga aggggctaac cgccgttaag tttgagatag caatcaagaa gaatagctac
    10561 acgtatgagt gagtgaaaga aaaggagaat catggtttta cctaccgttg ccattgtggg
    10621 ccgtccaaac gtgggcaagt ccaccttatt taatcgaatc gcaggagagc gcatttcaat
    10681 tgtagaagac gttgaagggg ttactcgtga ccgtatttat gctactgggg agtggcttaa
    10741 ccgccaattt tcattgattg atacaggtgg tattgatgac gttgatgccc cgtttatgga
    10801 gcaaatcaag caccaggctc aaattgccat ggaagaagct gatgttatcg tttttgttgt
    10861 ttctggcaaa gaaggggtta cggatgcaga tgaatatgtg tctaaaattc tgtatcgaac
    10921 caatacccca gtcattttag ctgttaataa ggttgataat cctgaaatgc gtaatgacat
    10981 ttatgatttt tattcacttg gtttagggga tccctatcca gtttcttctg ttcatggtat
    11041 cggaacaggg gatgtcttgg atgcgatcgt tgaaaacttg ccagtagaag aggctgaaga
    11101 aaatgatgat attattcgct tcagtttaat cggtcgtcct aatgttggga aatctagctt
    11161 gattaacgct attttaggtg aggaccgtgt tatcgctagc cctgtcgcag ggacaacacg
    11221 tgacgctatt gacacgcatt ttacggatgc tgacggtcaa gaatttacca tgattgacac
    11281 ggctggaatg cgcaaatctg gcaagattta cgaaaataca gagaaatatt ctgtcatgcg
    11341 tgccatgcgt gccattgacc gctccgatgt ggtcttgatg gttatcaatg ctgaagaagg
    11401 tatccgtgaa tacgacaagc gtatcgctgg ctttgcacac gaagctggta aaggaatgat
    11461 tatcgtggtt aacaaatggg ataccattga caaggataat cataccgttg ctaagtggga
    11521 agccgatatt cgtgaccaat tccaattttt gacatacgct ccgattattt ttgtgtcagc
    11581 tttgacgaag cagcgcctca acaaattgcc tgacttgatt aaacgtatca gtgagagtca
    11641 gaataaacgt atcccatctg ctgtcctcaa tgatgttatc atggatgcta ttgccattaa
    11701 tccaacgcca acggataagg gtaaacgttt gaaaattttc tatgccactc aagtgtcagt
    11761 aaagccacca acatttgtcg tctttgttaa cgaagaagaa ttgatgcact tctcttatct
    11821 acgcttcttg gaaaaccaga ttcgcgcagc ctttaccttt gaagggacac cgattcatct
    11881 gattgctcgc aaacggaaat aagaaatgaa catcaactaa aatgctgacc taagaaataa
    11941 tctcatcatt gataaggact gtgttgtgta tgttacagga gacagtcctt tttataatac
    12001 ctttaagggt gtagacggaa gattagtagc aaacaaagaa aaaggatttt gacaagaaat
    12061 atcagtcgct ttttgctata atgaaaggat aagcaaaaag gtggaaaaat gatggctaga
    12121 ttaattccag gacgagtaag gaatgaaggt attaagcttt atgagcaagg tttagtctca
    12181 ttccaagatg ataataaagg cattcttcaa atagaagtgg agacttatca ggttcaatat
    12241 ggagcagatg atgaggatat cacttgtcaa tgcgacacat ttcatatgaa acattattgc
    12301 aagcatattg cagcagtcga gtatttttta aaaaatgatc aaaaaggtaa gcttttttta
    12361 aaacagttaa caaatcaaac aaaaatcaaa gaaacaacaa agaaaatgac atcttttggt
    12421 agtctctttt tagatagctt agccatgaat gaagatgatt ctgttaaata caggctgtct
    12481 gctcttggta gtcgaagccc tttttcgtca gattactggt ggagtctgaa aatcaatcgt
    12541 ttaccagatg atcgttctta tgtgattcga gatattaaag gctttttgca gcttattaaa
    12601 aaagaaggct tttatcagat tggaaaaaat tattttgaac aactttcatg gctgcagttt
    12661 gacccatcca gtcaagacct tattgagttt ttatggcgct tagcatctaa tactgacaaa
    12721 ggggacaacg agaacatttt tccaaatcat gcaaggcatt tgcgcttacc tagtggtttt
    12781 tttgaagaag gtattcacta tttgacgtca ctttatgatt ttacttttga ggggccttct
    12841 caaacttatc atcatctttt tgtccgttct ttggaagcag aggcaggcct ttatgagttt
    12901 aaagtggaag tgcatcgcaa atctattgaa ttgcaaattg ctgaaaaaaa tgtgcaatac
    12961 ctctttgata atgactatct tctttatcaa gatacctttt atcacttgac gttaaagcaa
    13021 cgaaaaatgg ttcaggctat tcgtagtctg cccattgagg ccgacttggc taaacatatt
    13081 cattttgatt tggatgatca tgcgaaatta gctgcaagct tatcagattt taaacagatc
    13141 ggtctagtag aggctcctaa aagctttgct attagagact ttgaggtgac ttttcaattt
    13201 gatttgttaa atcgagatga aatcagttgt cagcttatgt ttgattatgg caattaccaa
    13261 gttagtgaca aagcaagttt agaagcactt ccttttgcta gtcatttaaa aaaagaagaa
    13321 aaaatcaatc gttctttact tgcttttggc ttctcgccac aattttactc aaaaaagcga
    13381 ctaacaagtg caaaagagct ctatactttt tttgaagaaa cagttccatg ttttgagcgc
    13441 ttaggtaatg ttgcgttgag tacagctatc caagcattac aagtgaaaga aatgcccaag
    13501 atcgctatta gacgcaatca gggattacta gatatttcgt ttgatttttc aaccattatt
    13561 gaaaatgata ttgaccaagc tgttacagct ctttttcaaa ataatcctta ttttgtgagt
    13621 caaacaggtc aattagtggt ttttgatgat gaaactcaaa aagttagcaa aagtttacaa
    13681 gaattaagag caagacagct aaaaaatggt cacttgcaac tggatggcat tagagctcta
    13741 caagtatcca aactttttga aggcatgaca tctgttcatt tttcaaagga attagaagag
    13801 ttagcctatc atttgcagca tcctgaaact ttttcaataa aaccgttacc agttaaggct
    13861 caaatgcgag attatcaacg caacggtgtt cagtggttat caatgctcaa tcattatggc
    13921 tttggtggca ttttagcaga tgacatggga cttggaaaaa ctctccagac acttgctttt
    13981 ttagctagtc acctgaaatc agatagtaaa gtgctcattt tatctccatc tagtcttatc
    14041 tataattggt ttgatgaatg tcaaaaattc acacctcagc tagatgttgt agtttcttac
    14101 ggacttaaac aaatcagaga tcaaatcatc gaagaaggtc atcaaataac cataaccagc
    14161 tattcctcct ttagacagga ttttgagacc tatcaagcgt ttcactacga ttatcttatc
    14221 ttagatgaag ctcaagtgat aaaaaatgcg caaactaaga tttcacactg tttacgtgcg
    14281 tttaatacag ccaattgttt tgcgctttcg ggcactccta ttgaaaataa aatgcttgaa
    14341 atttggtcca ttttccaaat tgttttgcca gggttgctac cgactaaaaa agagttttta
    14401 aaattaacag ctgagcaagt atcgcgctat atcaaaccct ttgtgatgcg tcgtaaaaaa
    14461 gaagatgtcc taccagaatt acctgattta attgaaacta attattcaaa cgagatgaca
    14521 gatgaacaaa aagctattta cttagcgcag ttaaggcaga tgaaagatca aattcgaaat
    14581 tctagtgatg tggatattag ccgtcaaaaa attgagattt tatctggtat cactcgattg
    14641 aggcaaatct gtgacactcc aagtctcttt atggattacc aaggcaaaag tggtaagcta
    14701 gatagtttgc gaatcttatt gacacaaatt aaagaaaatg gtcatcgggc tttgattttt
    14761 tctcaattta gagggatgct ggacttggcc aagcaagaga tgactgctct aggattgacc
    14821 tcttatcaaa tgacgggatc tacccctgcc aatgaacgtc aagaaatgac tagagccttt
    14881 aataacggtt ccaaggatgc ttttttgatt tccctcaagg caggtggagt tggaatcaac
    14941 cttacgggag cagacacagt gattttaatc gatttatggt ggaatcctgc tgttgaaatg
    15001 caggcaatca gtcgcgccta ccgtattggt caaaaagaaa acgttgaagt atatcgctta
    15061 atcactcgag ggacgattga agaaaagatt ttagagcttc aagaaagtaa gcgcaatttg
    15121 gtgactacag ttttggatgg caatgaaagc cgagcaagta tgtccattga agaaataaaa
    15181 gaaattttgg ggctaaacac tcattaatct tccttgattt tagtgacatt aaaaaccctt
    15241 taaagaagat gacaccaaaa gcaccttata taacctcgtt aaaacgcttc tttttgaaat
    15301 atctttattg atatagcgat tgtcacttgc tagtaatgat taaaacattt agaaaaagac
    15361 aagtcataga atcatcaagg aatgctttga aaaataacag tatttaagtt ataatagagg
    15421 atgctacaag aagggagaat caaaaaagga tggtaaaaaa tcagtttcct ttagttgcag
    15481 atggcattgc tattagtgat ccagcaaagc aaatggctct gtatgaaaat gaagatctca
    15541 ttaccaacat ccgtggctat taccaggaca aggagtatga tgatattgct agaaatgagg
    15601 agtttactgc taaagcaact tctcggcagg ccccttctag taagcgcttt tgtagcaatg
    15661 atgaaaaaca ccattatgtc aaagaggcgc gtcaaaaagc aaagcaagat ctcaaggaaa
    15721 aaagacaggc ttatcttgcc aaagaaatgg cctatgttcc taaacaggtt tctaaaaagc
    15781 aacagcctgc ggattcttct ccttctcaaa agcaagcaac aacagaaatg agtcgtttta
    15841 caaaaaagct tcatcaagac aactatattt tagctgagtt gccaaaagaa tataaagagc
    15901 caaaaaatct tcctcaacaa ggaactacaa aaaaaaataa ttacgatttt ttaaaatcca
    15961 gtcaaattta caataataaa gaaatgagac aacaacgaga aaaaacaatt gcgcaagagc
    16021 ttaatcttag taggtttgag gatcttcctt aataaatcta ctcagcgtat gcagatgaca
    16081 aaggagttaa gatgtcaaaa acgtatcatt ttatcggaat taaagggtca ggaatgagtg
    16141 ccttggccct tatgttgcat caaatggggc ataaggtgca aggtagtgat gttgaaaaat
    16201 actatttcac tcaaagagga ttggaacaag cgggcatcac tatcttacct tttagcgagg
    16261 acaacattac gccagatatg gaacttatcg ttgggaatgc ctttcgtgaa aacaacaaag
    16321 aagtggccta tgctttgaga caccagattc ctttcaaacg ttatcatgac tttttaggtg
    16381 attttatgaa atcatttatc agttttgcag tagcaggtgc tcatggtaaa acttctacaa
    16441 cgggtttatt gtcacatgtc ttgaaaaata tcacagacac atcttattta attggtgatg
    16501 gtactgggcg tggatctgct aatgcacagt attttgtttt tgaatcagac gaatacgaac
    16561 gccattttat gccttatcac ccagaatact ctatcattac taatattgat tttgaccatc
    16621 ctgattattt cacaggaatt gctgatgtgc gaaacgcttt caacgattat gctaagcagg
    16681 tcaagaaagc tttgtttgtt tatggtgaag atgacgaact aaaaaagatc gaagcacctg
    16741 cacctattta ctattatggt tttgaagagg gcaatgattt tatcgcttac gacattacgc
    16801 gtactacgaa cggatcggac tttaaggtaa aacaccaagg agaggtcatt ggtcagtttc
    16861 acgtacctgc ttatggaaaa cataacattt taaacgctac agcagtaatt gcaaacttgt
    16921 ttgtagccgg cattgatatg gctctagtgg cagaccatct taaaactttt tcaggtgtga
    16981 aacgtcgttt tacagagaaa attatcaatg acactattat tattgatgac tttgctcatc
    17041 atccaaccga aattgtggca acaattgatg cggctagaca aaaataccca agtaaagaaa
    17101 ttgtagcaat atttcagcca catactttta ctcgcactat tgccttatta gaggattttg
    17161 cttgtgcttt aaatgaagct gacagtgttt atttagccca gatttatggt tctgcacgtg
    17221 aagtagataa aggtgaagtt aaggttgaag atttggctgc taagattatc aaaccatcac
    17281 aagtagtaac ggtggaaaat gtctctcctc ttcttgatca cgataatgct gtttatgttt
    17341 ttatgggagc tggagatatt cagctatatg aacattcttt tgaggaatta cttgcaaatt
    17401 taactaaaaa taaccaataa catgtcctgc cttaggcagg ttttctcatt ctaaaggagt
    17461 ttgatatgtt gattagacag gttcaaggat ccgatttgga agttattgct accattgaaa
    17521 gtgacaattt ttcaccacaa gaagcaacga caagagcagt tcttgaagag cgcatacggc
    17581 tgataccaga tactttttta gtggcactta tcgatcagga aattgttggc tatatcgaag
    17641 gtccagttgt cactactcct attttggagg attctttgtt ccacggggtg actaaaaatc
    17701 ctaaaactgg aggctatatt gctattacta gtttgtccat agctaaacat tttcagcaac
    17761 aaggggttgg gacagctttg ttggcggcac taaaagattt agtagtagcg cagcaaagaa
    17821 caggtcttat tttgacttgt catgattact tgatttccta ttatgaaatg aatgggttta
    17881 ttaaccaagg aatttcagag tctcaacatg gtggaaccct ttggtaccaa atgatttgga
    17941 aactttagaa tcacagctga ttcaactgtt ggtttgttaa aaataagtaa caaatgcatc
    18001 atttaattgc aattaggata cttttaagtt ataatggcgg ttgattatat ttaggaggat
    18061 tggctttgac cgattttaag gataaagacc aacaagatca gcagcgaagc tttaaagagc
    18121 agattcttgc tgagttggaa aaagcaaatc aaatcagaaa agaaaaagag gaagaacttt
    18181 ttcaaaaaga gttggaagct aaagaagcag ctaagagaac ggcccagcta tatgctgaat
    18241 ataaaaggca agatgctttt caaaaagagt ctatagcaca taacaataag acagctaaac
    18301 actttcaagc tataaaaggt gcggtaatga cttcagaagc gcttaaaccg actttacttt
    18361 ctgaaaaaga aaactcatct ttaaaaacga caaataagag agtcgtgcag gcaaatgagc
    18421 ttcaagagac tgcctctaaa gaatctcaag taccgttaac tattgagaaa ggtcattcag
    18481 tgagacgaaa attaagcaaa cgccaacaga ctgagcgagc tgctaaaaag atttcaaccg
    18541 ttttgattag ttctattatt ataacccttt tggctgttac tctagcagga gcaggctatg
    18601 tttatagcgc tttaaatcct gttgataaaa atagtgatgc ctttgttcaa gttgagatac
    18661 catctgggtc aggcaataaa ttgattggtc aaattcttca aaaaaaaggt ttaatcaaga
    18721 atagcactgt ttttagtttt tatacaaaat ttaaaaactt tacaaatttt cagagcgggt
    18781 attacaatct gcaaaaaagt atgagtctag aagaaattgc tagtgcttta caagaaggtg
    18841 gtacagcaga acctaccaag ccatctcttg ggaagatctt gattccagaa ggatacacga
    18901 ttaaacaaat agctaaagct gttgagcata atagcaaggg aaagaccaaa aaagctaaaa
    18961 caccttttaa cgagaaggat tttttggatt tagtcacaga tgaggctttt attcaagata
    19021 tggtaaaaag atatccaaaa ttattagcaa ctatcccaac taaagaaaaa gctatttacc
    19081 gtttggaagg ttaccttttc ccagcaacct ataactatta caaagaaact accatgagag
    19141 aacttgtaga ggacatgctg gcagctatgg atgctacttt ggtaccctat tatgataaaa
    19201 ttgctgctag tggtaagaca gtcaacgagg tattgacctt ggcctctttg gttgaaaaag
    19261 aaggttcaac agacgatgac agacgtcaaa ttgcaagtgt cttttataac cgccttaata
    19321 gcggaatggc actacaatct aatatagcta ttttgtatgc gatggggaaa cttggtgaga
    19381 aaacaacctt ggctgaggat gctactattg acaccaccat taattctcct tataatattt
    19441 ataccaatac aggtctgatg cctggtccag ttgctagctc gggggtttct gcaattgaag
    19501 caaccctaaa tccagcctca acggattatt tatactttgt ggccaatgtc catactggtg
    19561 aagtttacta tgcaaaaaca tttgaagaac actctgcaaa tgttgaaaaa tatgtgaata
    19621 gtcaa
//
LOCUS       DAUCLR010000029        19156 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.029, whole genome shotgun sequence.
ACCESSION   DAUCLR010000029 DAUCLR010000000
VERSION     DAUCLR010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19156
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.029"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..840)
                     /gene="galU"
                     /locus_tag="VGT87_000868"
     CDS             complement(<1..840)
                     /gene="galU"
                     /locus_tag="VGT87_000868"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEQ3813741.1"
                     /translation="MTKVRKAIIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEE
                     ALKSGIEEILIVTGKSKRSIEDHFDSNFELEYNLQAKGKIELLKLVDETTSINLHFIR
                     QSHPRGLGDAVLQAKTFVGNEPFVVMLGDDLMDITNPNVKPLTKQLIDDYEETHAATI
                     AVMRVPHEDVSNYGIIAPQAKAVKGLYSVDTFVEKPQPQDAPSDLAIIGRYLLTPEIF
                     SILEKQEPGAGNEVQLTDAIDTLNKTQRVFAREFKGKRYDVGDKFGFMKTSLDYALKH
                     PQVK"
     gene            complement(873..1889)
                     /locus_tag="VGT87_000869"
     CDS             complement(873..1889)
                     /locus_tag="VGT87_000869"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEQ3813742.1"
                     /translation="MTKQKVAILGPGSWGTALSQVLNDNGHDVRLWGNIPDQIEEINT
                     KHTNRHYFKDIVLDKNITATLDLGQALSDVDAVLFVVPTKVTRLVARQVAAILDHKVV
                     VMHASKGLEPETHERLSTILEEEIPAHFRSEVVVVSGPSHAEETIVRDITLITAASKD
                     IEAAKYVQSLFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGYGDNAKAAVI
                     TRGLAEITRLGVKLGADPLTYSGLSGVGDLIVTGTSVHSRNWRAGAALGRGEKLEDIE
                     RNMGMVIEGIATTKVAYEIAQDLGVYMPITTAIYKSIYEGADIKESILGMMSNEFRSE
                     NEWH"
     gene            2187..2636
                     /locus_tag="VGT87_000870"
     CDS             2187..2636
                     /locus_tag="VGT87_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEQ3813743.1"
                     /translation="MSQVIGDLRELIHQIEQISDEIAKKYDVEHLAGPQGYVLVFLAK
                     HQNQEIFVKDIEKQLRISKSVASHLVKRMVKNGFINVMPSQVDKRYKQVVLAQVGRDK
                     LPLLRECRKDIEHYFLKEITKEELLTAKKVIEQLKQNMLTYKGDNDA"
     gene            2629..4335
                     /locus_tag="VGT87_000871"
     CDS             2629..4335
                     /locus_tag="VGT87_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ3813744.1"
                     /translation="MLKDAILRYKWYALGSFFMVIGVVASSLLQPHYLKSVLSAVIQN
                     NQEKIYEVGKPLLIIALLGLMSGAINTVLAAKIAQGVSADMREKTFRKIQDFSYANIE
                     AFNAGNLVVRLTNDINQIQSLVMMMFQILFRLPFLFIGAFIMAVQTFPQLWWVIVVMV
                     ILIALIMGLVMRQMGPRFGKFQRLMDKINRIAKENLRGVRVVKSFVQEQQQYTKFKET
                     SNDLLALNLSIGYGFSLMQPALMLVSYLAVYVSINVVSTMVETDPTVIGNIASFMTYM
                     MQIMFSIIVVGSMGMQVSRAFVSMARIRQILSTEPAMTFENEKEETISGSIVFDDVSF
                     TYPNDDEPTLKHISFAIEPGQMVGIVGATGSGKSTLVQLIPRLFDPQDGQILLGGKPI
                     KTLSQTTLRQSVSIVLQKAILFSGTIADNLRQGSAKADIDAMQKAAQIAQAKEFIDRM
                     DSRYESQVEERGSNLSGGQKQRLSIARGVINHPKILILDDSTSALDAKSEKRVQEALS
                     HKLEGTTTVIVAQKISSVVKADKILVLDQGQLIGEGTHAELVANNAIYREIYETQKGR
                     EE"
     gene            4338..6122
                     /locus_tag="VGT87_000872"
     CDS             4338..6122
                     /locus_tag="VGT87_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ3813745.1"
                     /translation="MKTARFFWFYFKRYRFSFTVIAVAVILATYLQVKAPVFLGESLT
                     ELGKIGQAYYVAKMSGQTHFSPDLSAFNAVMFKLLMTYFFTVLANLIYSFLLTRVVSH
                     STNRMRKGLFGKLERLTVAFFDRHKDGEILSRFTSDLDNIQNSLNQSLVQVVTNIALY
                     IGLVWMMFRQDSRLALLTIASTPVALIFLVINIRLARKYTNIQQQEVSALNAFMDETI
                     SGQKAIIVQGVQEDTMTAFLKHNERVRQATFKRRLFSGQLFPVMNGMSLINTAVVIFV
                     GSTIVLSDKSMPAAAALGLVVTFVQYSQQYYQPMMQIASSWGELQLAFTGAHRIQEMF
                     DETEEVRPQNAPAFTSLKEAVAINHVDFGYLPGQKVLSDVSIVAPKGKMIAVVGPTGS
                     GKTTIMNLINRFYDVDAGSITFDGRDIRDYDLDSLRQKVGIVLQESVLFSGTITDNIR
                     FGDQTIGQDMVETAARATHIHDFIMSLPKGYNTYVSDDDNVFSTGQKQLISIARTLLT
                     DPEVLILDEATSNVDTVTESKIQRAMEAIVAGRTSFVIAHRLKTILNADHIIVLKDGK
                     VIEQGNHHELLHQKGFYAELYHNQFVFE"
     gene            6240..7007
                     /locus_tag="VGT87_000873"
     CDS             6240..7007
                     /locus_tag="VGT87_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEQ3813746.1"
                     /translation="MIDLQEILANMNPNQKINYDRVMQQMAKVWAKESVRPSILMHVC
                     CAPCSTYTLEYLTQFADITVYFANSNIHPKDEYHRRAYVTQQFVSEFNAKTGNTVQFL
                     EADYVPNEYVRQVRGLEEEPEGGDRCRVCFDYRLDKTAQKAVELGFDYFASALTISPH
                     KNSQTINDVGIDVQKVYTTKYLPSDFKKNNGYRRSVEMCEEYDIYRQCYCGCVYAAKM
                     QGIDLVQVKKDAKAFMADKDLDNDFTHIRFSYRGDEM"
     gene            7117..7563
                     /locus_tag="VGT87_000874"
     CDS             7117..7563
                     /locus_tag="VGT87_000874"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEQ3813747.1"
                     /translation="MTKIRGFELVSSFTNPDLLPKRETTHAAGYDLSVAEAVTIAPGE
                     IKLVPTGVKAYMQDGEVLYLYDRSSNPRKKGIILINSVGVIDADYYGNEANEGHIFAQ
                     MQNITDHPVTLAVGERIVQGVFMPFLIADGDQARGERTGGFGSTGQ"
     gene            7644..9005
                     /gene="radA"
                     /locus_tag="VGT87_000875"
     CDS             7644..9005
                     /gene="radA"
                     /locus_tag="VGT87_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEQ3813748.1"
                     /translation="MAKKKATFICQECGYQSPKYLGRCPNCSAWSSFVEEVEVKEVKN
                     ARVSLAGEKSRPVKLKDVDNISYHRTQTDMSEFNRVLGGGVVPGSLILIGGDPGIGKS
                     TLLLQVSTQLANKGTVLYASGEESAEQIKLRSERLGDIDNEFYLYAETNMQAIRTEIE
                     NIKPDFLIIDSIQTIMSPDITGVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSAVVVTMEGSRPILAEVQSLVTPTVFGNARRTTTGLDFNRVSLI
                     MAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTSPQEAFLGE
                     IGLTGEIRRVTRIEQRINEAAKLGFTKVYAPKNALQGIDIPQGIEVVGVTTVGQVLKA
                     VFS"
     gene            9194..9691
                     /locus_tag="VGT87_000876"
     CDS             9194..9691
                     /locus_tag="VGT87_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEQ3813749.1"
                     /translation="MSYFEHFMAANQAYVALHGTAHLPLKPKTKVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFTNEGF
                     AKHIHEHLGVDVSGQDFLPFQDVEDSVRKDMAKLRASSLIPDDVVINGAVYDVDTGKM
                     TQVME"
     gene            9822..10532
                     /locus_tag="VGT87_000877"
     CDS             9822..10532
                     /locus_tag="VGT87_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HEQ3813750.1"
                     /translation="MVDNRMRFTIDQNMQFPLVEIDLEHGGSVYLQQGSMVYHTENVT
                     LNTKLNGKGSGLGKLVGAIGRSMVSGESMFITQAMSNGDGKLALAPNTPGQIVALELG
                     EKQYRLNDGAFLALDGSAQYKMERQNIGKALFGGQGGLFVMTTEGLGTLLANSFGSIK
                     KITLDGGTMTIDNAHVVAWSRELDYDIHLENGFMQSIGTGEGVVNTFRGHGEIYIQSL
                     NLEQFAGTLKRYLPTSSN"
     gene            10714..12159
                     /locus_tag="VGT87_000878"
     CDS             10714..12159
                     /locus_tag="VGT87_000878"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEQ3813751.1"
                     /translation="MSKPIRVRYAPSPTGLLHIGNARTALFNYLYARRHGGTFIIRIE
                     DTDRKRHVEDGERSQLENLKWLGMDWDESPETHENYRQSERLALYQQYIDKLLAEGKA
                     YKSYVTEEELAAERERQEAAGETPRYINEFIGMSADEKAKYISEREAAGIVPTVRLAV
                     NESGIYKWTDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVVDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYM
                     PEAVFNFIALLGWNPGGEEEIFSREQLIALFDENRLSKSPAAFDQKKMDWMSNEYLKH
                     ADFETVYALCKPFLEEAGRLTEKAEKLVELYKPQLKSADEIIPLTDLFFSDFPELTEA
                     EKEVMAGETVSTVLQAFKAKLEAMSDEDFKPENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPNTIYLLGRDKSIEHIKNML"
     gene            12553..13899
                     /locus_tag="VGT87_000879"
     CDS             12553..13899
                     /locus_tag="VGT87_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ3813752.1"
                     /translation="MANSEYMFLLLSIIVYYMTKIYIFSFLSDITLPVWKQLTILALA
                     LFFNQFPYLSPLLIDPLLFLVVLRQETKQLFSLKALFLAVAPSVLVDLLSRFMGTIVI
                     PYLFLSSGIYLGHIIFDLLAYLLIFPSFAIINYMIGKDYKVICQSGYSKRSHNFYQTL
                     LIFVLVYYVDIFVILGFTDPFLHFHHSLFVPTPYKLLFLMFILLLVYLLSYFNHSSKE
                     YLKNELRREQQAYMTNLETYGKHLEKLYRDVRAFQSDYLSRIERLGQAIKSESITQIQ
                     DIYAQTVHEANDYWDDKHYNISKLRKINISSIKSLLSAKIISAEKSGIDLNVEVPDNI
                     KETYIPELDLLLLMSIFCDNAIEAALEAQQPHMSIAYFLLDDYQMFVVTNTTKKKVDI
                     NKIFEEGYSSKGSERGIGLSNAQRILKKYPYLSLRTKSFDKEFSQTLTMPKEEVDR"
     gene            13896..15179
                     /locus_tag="VGT87_000880"
     CDS             13896..15179
                     /locus_tag="VGT87_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ3813753.1"
                     /translation="MMVIYSMALFYATIFINSWVIFAKVSAIKLSWKRVSVIGISFVI
                     ANMIFDKVILIDQLFFIIVSLLSAPKKKLFEHMFNGFFTILIVELLFRVIGSFFLPAV
                     LGFSIGQINNNLKLLELCYLFVLPIFYLFSYIFSIDLSLIRFISEDKMKKWVFWMNTA
                     MFSYYFFAHFLVTVQSGFLALYFQYRSILVFIYLAIFIWVIVKLDRFAKDQLSQKLTQ
                     AQNERIAYLENYNQSIEQLYREIRTVKHDSENILISLKDSIDSGDIDLITRVYDTVIQ
                     QSATSMMRTNYEISSLDNIKETVIRSIMNSKLLEAQYLGIELYIEIPDVIDHLPIKLI
                     DLIVLFTGLVDNAIETAKGSRRPFLSIAYFKQDNKQLFIIENSTKTNRVDIAKRFDAQ
                     QQNSAHFLTVLDSYPQITLSTKSDHYRLRQLLEMR"
     gene            15183..15923
                     /locus_tag="VGT87_000881"
     CDS             15183..15923
                     /locus_tag="VGT87_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ3813754.1"
                     /translation="MMNIFILEDDFIQQTRIESIVVGILKETRIPCNQLEVFSTPQKL
                     FESIQERGDHQLYFLDIEIGEYTRCGLELAAAIRQKDPNAVIVFVTTHSEFAPISFKY
                     KVSALDFIDKAGGQKQFKEQIEECIRYTYDMMSSRESKDMFLFETPQTRLKLPYKDIL
                     YFATATTPHKVCLWTQTERLEFYGNLSEIQAVAPKLFLCHRSYLVNLDKVVRIDKSKQ
                     LLYFENGDSCMVSRLKMKCLFERWEALR"
     gene            16463..16822
                     /gene="rnpA"
                     /locus_tag="VGT87_000882"
     CDS             16463..16822
                     /gene="rnpA"
                     /locus_tag="VGT87_000882"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEQ3813755.1"
                     /translation="MKKTYRVKREKDFQAIFKDGKSTANRKFVIYHLNRGQDHFRVGI
                     SVGKKIGNAVTRNAVKRKIRHVIMALGHQLKSEDFVVIARKGVESLEYQELQQNLHHV
                     LKLAQLLEKGFESEEKH"
     gene            16788..17615
                     /locus_tag="VGT87_000883"
     CDS             16788..17615
                     /locus_tag="VGT87_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEQ3813756.1"
                     /translation="MRKVLRVKKNIKIARIVPLVLLLVACGRGEVTAQSSSGWDQLVY
                     LFARAIQWLSFDGSIGVGIILFTLTIRLMLMPLFNMQIKSSQKMQDIQPELRELQKKY
                     AGKDTQTRMKLAEESQALYKKYGVNPYASLLPLLIQMPVMIALFQALTRVSFLKTGTF
                     LWVELAQHDHLYLLPVLAAVFTFLSTWLTNLAAKEKNVMMTVMIYVMPLMIIFMGFNL
                     ASGVVLYWTVSNAFQVLQLLLLNNPFKIIAERQRLANEEKERRLRERRARKKAMKRK"
     gene            17627..18541
                     /locus_tag="VGT87_000884"
     CDS             17627..18541
                     /locus_tag="VGT87_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEQ3813757.1"
                     /translation="MVLFTGKTVEEAIETGLQELGLSRLKAHIKVISKEKKGFLGFGK
                     KPAQVDIEGISDKTVYKADKKATRGVPEDINQKNAPVVNSADVEPEEIKATQRLEAED
                     TKVVPLMSEDSPAQTPSNLAETVTERKAQQPSIPVEESEVPQDPGNDGFSKDIEKAAQ
                     EVSDYVTKIIYEMDIEATVETSNNRRQINLQIETPEAGRVIGYHGKVLKSLQLLAQNF
                     LHDRYSKNFSVSLNVHDYVEHRTETLIDFTQKVAKRVLESGQDYTMDPMSNSERKIVH
                     KTVSSIEGVDSYSEGNDPNRYVVVSLQR"
     gene            18856..18990
                     /gene="rpmH"
                     /locus_tag="VGT87_000885"
     CDS             18856..18990
                     /gene="rpmH"
                     /locus_tag="VGT87_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEQ3813758.1"
                     /translation="MKRTYQPSKIRRQRKHGFRHRMSTKNGRRVLAARRRKGRKVLSA
                     "
BASE COUNT         5830 a         3392 c         4008 g         5926 t
ORIGIN      
        1 tttgacttga gggtgtttca gggcataatc caaagaagtt ttcataaacc caaatttatc
       61 gccaacatca tagcgtttcc ctttaaactc acgagcaaag acacgctgtg tcttattgag
      121 ggtatcaata gcatcagtca gttgaacttc attgcctgca ccaggttctt gtttttctaa
      181 aatactaaaa atttctggtg tgagcaagta acgtccaata attgccaaat cactaggtgc
      241 atcttgaggt tgtggttttt caacaaaagt atccacacta tagagtcctt ttactgcttt
      301 tgcttgagga gcaatgatac cgtagttaga gacatcctcg tgaggaaccc tcatgacggc
      361 aatagttgca gcatgagttt cttcatagtc atcgatcaat tgtttggtta atggttttac
      421 atttggattt gtaatatcca tcagatcatc acctagcatg acaacaaaag gttcattgcc
      481 aacaaaggtc tttgcttgaa gaactgcgtc tcctaatcct cttggatgac tctgtcgaat
      541 aaagtgaaga ttaatagaag tggtctcgtc tactaacttt agaagttcga tttttccttt
      601 agcctgtaaa ttatattcta attcaaaatt agaatcaaaa tgatcttcta tggagcgttt
      661 ggattttcca gtaacgataa gaatttcttc gatacctgat tttagagctt cttcaacgat
      721 aaattgaata gttggtttat caacaatagg tagcatctcc ttggcaaggg ccttggttgc
      781 aggtaaaaaa cgtgtgccta aacctgctgc gggaataata gcttttctga ctttggtcat
      841 cagcacctcc tcttatttta gtgacgtatg gcttaatgcc attcattttc agaacgaaat
      901 tcatttgaca tcatgccgag gatgctttct ttgatatcag cgccctcgta gatggactta
      961 taaatagctg ttgtaattgg catgtaaact ccgaggtctt gggcaatttc ataggctacc
     1021 ttagttgttg cgatgccttc gataaccata cccatgttgc gttcaatatc ttctaatttt
     1081 tcaccacgtc caagggcagc tccagcccgc cagttacgtg aatggacaga agtacctgtt
     1141 acaattagat caccaacacc agataaccca ctgtaggtta acgggtcagc accaagcttg
     1201 acgcctagac gggtgatttc agctaaccca cgagtaatta ccgctgcttt ggcattatct
     1261 ccatacccaa gtccatggag agctcctgct ccaacagcaa taatgttttt gagggcacca
     1321 gctgtttcaa ctccgatgac gtcggtattg gtataaagtc ggaagtagtg gttactaaag
     1381 agggactgaa catactttgc agcttcaata tcttttgagg ctgccgtgat gagagtaata
     1441 tcgcgaacga tagtttcttc agcatgacta ggacctgata caacgaccac ttcgctacga
     1501 aaatgagctg gaatttcttc ttctaggata gttgataaac gttcgtgggt ctctggttct
     1561 agaccttttg aggcatgcat gaccactact ttatgatcta aaatggcggc aacttgtcta
     1621 gcaactaatc gtgttacttt agttggcaca acaaaaagca ccgcatcaac atctgatagg
     1681 gcttgaccta aatcaagagt tgccgtaatg tttttgtcta acacaatgtc tttgaagtag
     1741 tgtctattgg tatgcttggt atttatctct tcaatttgat caggaatatt tccccataaa
     1801 cggacgtcat ggccattgtc attaagaacc tgagaaagag cagttcccca agaaccaggt
     1861 cctaaaatag ctactttttg ttttgtcatt ctaaattctc cttcaagagc tagtcactct
     1921 attataacat aacaccttga ttagctctat tagaaaaggt atatggtctt ggctaataag
     1981 gcgttctcaa gagagctagc tatattaata atgttgttta ataagcttca tcaggtgagc
     2041 tgttgtcatg gctttgcctt gttcttttta gttatttaag ttcggaaact tatttttaaa
     2101 aataaagata ttttaattga caatagttat ctagagaact aaaatactta tcatagaaac
     2161 tattaaggaa aagaaggagg gaagagatgt cacaagtgat aggtgattta cgtgaattga
     2221 tacatcaaat cgaacaaatt agtgatgaga ttgcaaaaaa atatgatgta gagcatctag
     2281 caggtcctca aggttatgtt cttgtttttt tagctaaaca ccaaaatcaa gaaatatttg
     2341 tcaaagatat tgaaaaacaa cttcgtatct caaagtcagt tgctagtcat ttagtgaaac
     2401 gtatggtcaa aaatgggttt atcaatgtga tgccttccca agtggataag cgttataagc
     2461 aagtagtgtt agcgcaggtt ggtagagata aattgccttt gttgcgggag tgtcgtaagg
     2521 atatcgagca ctatttttta aaagaaatta caaaagaaga gttgctgaca gcgaaaaaag
     2581 taattgaaca gctcaagcaa aatatgctaa cttataaagg agacaacgat gcttaaagac
     2641 gcaatcttac gttataaatg gtatgcttta ggctcatttt ttatggttat tggggttgtt
     2701 gcaagtagtc tcttgcaacc acattatctt aaatctgtgc taagcgctgt tattcaaaat
     2761 aatcaagaga aaatttatga ggttgggaaa ccattactta ttattgccct tttagggtta
     2821 atgtctggag cgattaatac ggttttagct gctaaaatag ctcaaggtgt atctgctgat
     2881 atgagggaga agacctttcg aaaaatccaa gacttttcat atgccaatat tgaagctttt
     2941 aatgctggta atcttgtggt taggctaact aatgatatca atcaaatcca aagtttagtc
     3001 atgatgatgt ttcaaattct ctttcgtttg ccgttccttt ttattggcgc gtttatcatg
     3061 gcagttcaga cattccctca attatggtgg gtcattgttg taatggttat cttaattgcc
     3121 ctcatcatgg gtttggtgat gcgtcaaatg ggtcctcgtt ttggtaaatt tcaacggtta
     3181 atggataaga ttaatcgcat cgctaaggaa aatctccgtg gcgttcgcgt ggttaaatca
     3241 tttgtacaag aacaacaaca atacactaaa tttaaagaaa cgtccaatga tcttttggcg
     3301 ttaaatttat ctattggtta tggtttttca ctaatgcagc cggctttaat gttggtgtct
     3361 tatttggcag tttatgtttc tataaatgtt gtttcgacca tggtcgaaac agatccaaca
     3421 gtgattggga atattgcctc tttcatgact tatatgatgc aaattatgtt ttcaattatt
     3481 gtggttggtt ccatggggat gcaagttagt cgtgcttttg tttctatggc tagaattagg
     3541 caaatattgt ctacagaacc tgccatgaca tttgaaaatg aaaaggaaga aaccatctca
     3601 ggaagcattg tatttgacga tgttagtttt acatatccta atgatgatga gccaaccttg
     3661 aagcacattt cttttgctat tgaacctggg caaatggtcg gcatcgttgg tgcaacagga
     3721 tctgggaaat caacgcttgt acagctcatc cctcgcttat ttgatcctca agatggccaa
     3781 attttgttag gaggaaagcc gattaaaacc ctaagtcaaa caaccctgcg ccaatcggtt
     3841 tccatcgtgt tgcaaaaggc tattctcttt tcaggaacga tagctgataa ccttcgccaa
     3901 ggctcagcaa aagctgatat tgatgctatg caaaaagccg ctcaaatcgc gcaagcaaaa
     3961 gaatttattg atcgcatgga tagccgttat gagagccaag ttgaagagcg aggtagtaac
     4021 ttgtctggcg gacaaaagca acgtctgtct attgcacgtg gtgtcattaa tcatcccaaa
     4081 atccttatat tagacgattc cacttcagct cttgatgcaa aatctgaaaa acgcgttcaa
     4141 gaagccttaa gtcataaatt agaaggtaca actacagtta ttgttgctca aaaaatttca
     4201 tcggtagtta aggcagataa gatcttagtc cttgaccaag gacagttaat tggagaagga
     4261 acacacgcag agttggtggc taataatgcg atttatcgtg aaatttatga gacacaaaaa
     4321 ggaagggagg agtagtgatg aaaacagcac gttttttctg gttttatttt aaacgctatc
     4381 gtttctcgtt tactgtcatt gctgttgccg ttatcttagc aacttattta caagtaaaag
     4441 ctcctgtctt cttaggggag tccttgactg agttgggaaa aatcggtcag gcttattacg
     4501 ttgctaagat gagtggtcag acacatttta gccctgattt atcagctttt aatgccgtga
     4561 tgtttaagct tttgatgact tatttcttta ctgttttagc taatctaata tatagtttct
     4621 tactcacacg tgttgtctca cattcgacca accgcatgcg caagggcttg tttggtaaat
     4681 tagaacgttt aaccgtcgcc ttttttgacc gccataaaga tggggagatt ctttctcgtt
     4741 tcacgagtga tttggataat attcaaaact cgctgaacca atccttggtt caagtggtga
     4801 ctaatattgc cctttacatc ggcctggtct ggatgatgtt taggcaagat agccgtttag
     4861 ctttgttaac catcgcatca accccagttg ctctcatttt tttagtgatt aacatccgtt
     4921 tggcaagaaa atacaccaat atccaacagc aagaagtcag tgctttaaat gcttttatgg
     4981 atgaaaccat ttcaggacaa aaggctatta ttgtacaagg tgtccaagaa gatacgatga
     5041 cagccttttt aaagcataat gaaagggttc gacaagccac cttcaaacgc cgtctgttct
     5101 caggacaatt atttccagtc atgaatggaa tgagccttat taacacggct gtcgtgattt
     5161 ttgtcggttc aacaattgtc ctcagtgaca aatctatgcc agcagcggca gcgcttggtt
     5221 tagtggttac ttttgtacaa tattcccagc aatattacca acccatgatg caaatcgcgt
     5281 ctagttgggg agaattgcag ctggccttta ccggtgctca ccgtattcaa gaaatgtttg
     5341 atgaaaccga agaagttcgt ccacaaaatg caccagcgtt caccagctta aaagaagcag
     5401 tggcgattaa ccacgtcgat tttgggtatc ttcctgggca aaaagtttta tcagatgtgt
     5461 caatcgttgc acccaagggc aaaatgattg ccgtggttgg accgacaggt tctggaaaga
     5521 ccactattat gaacttgatt aaccgtttct acgatgtgga tgcaggttcg attacctttg
     5581 atggccgtga tattcgtgac tacgatttgg atagtcttcg tcaaaaggta gggattgtgt
     5641 tgcaagagtc agttcttttt tcaggaacca ttacggataa tattcgtttt ggtgatcaga
     5701 ccattggtca agacatggtt gaaactgctg cgcgtgcgac ccatattcat gactttatca
     5761 tgtccttacc aaaagggtac aatacctatg tctcagatga tgacaatgtc ttttcaacag
     5821 gtcaaaagca gttgatttct attgctagga cgctactgac tgaccctgaa gtgttgattt
     5881 tggatgaggc cacttcaaat gttgatacgg ttaccgaaag taaaattcaa cgggccatgg
     5941 aagctatcgt ggcaggtcga actagctttg tcattgctca ccgcctcaaa accattttaa
     6001 atgcagatca cattattgtg ttgaaagatg gcaaggtcat tgagcaagga aatcatcatg
     6061 agctattgca tcaaaaaggc ttttatgccg aattgtatca caatcaattt gtctttgaat
     6121 agatggtatc agctccgcgt cttagcggag ttttttggtg aacaataaag cagtcaagct
     6181 ttctttcctg atatggtata atagcaagca agagaaaaaa caattagaaa gtagccacaa
     6241 tgattgattt acaagagatt ctcgctaaca tgaatcccaa tcaaaagata aattatgaca
     6301 gggtcatgca acagatggcc aaggtttggg ctaaggaatc cgtgcgtccc agtattttaa
     6361 tgcatgtctg ctgtgcccca tgctccactt acaccctaga atacctgacg cagtttgctg
     6421 acattacggt ttactttgcc aattctaata ttcaccctaa ggacgaatac catcgcagag
     6481 cctatgtgac acaacagttt gtctccgagt tcaacgctaa aacaggcaac accgtgcagt
     6541 ttctcgaagc ggactatgtc cccaatgagt acgttcgaca agtgcgaggt ttagaagaag
     6601 aaccagaagg tggggaccgt tgccgtgtct gttttgatta ccgactagac aagaccgctc
     6661 aaaaagcagt ggaactaggt tttgactact ttgccagtgc cctaaccatc agtcctcata
     6721 aaaattcgca aaccattaat gatgtgggca tcgatgtgca aaaggtttac accaccaaat
     6781 acctgccaag tgatttcaag aaaaacaacg gttatcgcag atccgttgaa atgtgtgagg
     6841 agtatgatat ttaccgtcaa tgctattgtg gctgtgttta tgctgccaaa atgcaaggta
     6901 ttgacttggt tcaagtgaaa aaagatgcca aagcttttat ggcagacaag gatttggata
     6961 atgatttcac ccacatccgc tttagttatc gtggggatga gatgtgatgg catgattaaa
     7021 agtggtccgt ttaaagttga ccgctttttt gctattcttt tacgaatagt ctataataag
     7081 aggatatgat tgacttgaaa ggatatacct atcataatga caaaaattcg tggtttcgag
     7141 cttgtttcaa gctttactaa tcctgattta ctaccaaaac gtgagacgac tcatgcagct
     7201 ggttatgacc tgtctgttgc tgaggctgta accattgccc caggggagat taagttggta
     7261 ccaacagggg ttaaggccta tatgcaagat ggtgaggtgc tttacctcta tgatcgttcg
     7321 tcaaatccac gaaaaaaagg gattatcctg attaattcag taggtgtgat tgatgcagat
     7381 tattatggca atgaggctaa tgaaggacat atttttgcac aaatgcaaaa cattacagat
     7441 caccctgtga cattagctgt gggtgagagg atcgttcagg gtgtttttat gcctttttta
     7501 attgctgatg gtgaccaagc cagaggtgaa cgaacaggtg gttttggttc gacagggcag
     7561 taaagcgatt taacaaaaat gcttatgaca gcagacctat tctttaagag tgggtttgtc
     7621 ttgtctaggg gagggagatt actatggcaa agaaaaaagc aacctttatc tgtcaggagt
     7681 gtggctacca gtcgccaaag tatttagggc gctgtccaaa ttgctcggcc tggtcttctt
     7741 ttgtggaaga agtagaggtc aaagaagtca aaaatgcccg tgttagtcta gctggagaaa
     7801 agtccagacc ggttaaacta aaagatgtgg ataatattag ttatcacaga acacagactg
     7861 acatgtctga atttaataga gttcttggag ggggtgtggt tcctggaagc ttgattttaa
     7921 ttggtggtga tccaggtatt ggaaaatcca ccttactctt gcaggtatct acgcagctcg
     7981 ctaataaggg aacggttctc tatgcgtctg gggaagaatc agcagagcag attaaactgc
     8041 ggagtgagcg tctgggtgat attgacaatg aattttacct ctatgccgaa accaatatgc
     8101 aagctattcg cacagaaatt gaaaatatta aaccagattt tttaattatt gactccattc
     8161 agaccatcat gagccctgat attacaggtg ttcaaggatc agtcagtcag gtgagagaag
     8221 tcactgcaga attgatgcag cttgcaaaga cgaacaacat tgcaaccttt attgtgggtc
     8281 atgtcaccaa ggaaggaacc ttggctgggc ctcgtatgtt agaacacatg gtggataccg
     8341 ttctctactt tgaaggggaa aggcatcaca cctttcgcat tcttcgggct gtgaaaaacc
     8401 gctttggctc cactaacgag attggcatct ttgaaatgca gtctggcggc ttggtagagg
     8461 tcttaaatcc tagtcaagtc tttttagagg aacgcttaga tggggcaaca ggatcggctg
     8521 tcgttgtaac gatggaagga agtcgtccta tcttagcaga ggtgcaatct ttagtgacac
     8581 caaccgtttt tggcaatgcc agacgaacca ctacgggact tgatttcaat cgcgtaagcc
     8641 taataatggc agttttagaa aagcgttgtg gtttactctt acaaaatcaa gatgcctacc
     8701 tcaagtcggc tggtggtgtc aaactagacg aacctgccat tgatttggca gtcgctgtgg
     8761 ctatcgcatc cagttataag gaaaaaccaa ccagccctca agaagctttc ttaggcgaga
     8821 tcgggttaac aggtgagatt cggcgagtga cccgtattga gcaacggatt aatgaagcag
     8881 caaagcttgg ctttaccaag gtctatgctc ctaaaaatgc cttgcaaggc attgatatcc
     8941 ctcaaggtat tgaggttgtt ggagtgacta cggttggtca ggttttaaag gcagttttta
     9001 gctagaggat cagaaactaa gagcaaatga ggaggaaatt tgtcaagaaa gtatcccaat
     9061 catttgtttt tttaactttt cgaacgatac cgaccgtcta agaggttaat ccagctattt
     9121 tccataaaag atgttaagat aagacaaact aacagcaaaa atagctctgc tgtttctcaa
     9181 aaggagttta ttgatgtctt attttgaaca ttttatggct gccaatcagg cttatgtggc
     9241 cttgcatggg acagctcatt tgcccttaaa acccaagact aaagtagcga ttgtgacttg
     9301 tatggattca cggctacatg tggcccaagc tctggggctg gctctaggag atgctcatat
     9361 tttaaggaat gctggcggac gtgtcaccga agacatgatt cggtcgctgg ttatttctca
     9421 gcagcaaatg ggaacgcgag agattgtggt tctccaccat acagattgtg gcgcgcagac
     9481 ttttactaat gaaggctttg ccaagcatat tcatgagcac ttaggtgtgg atgtttcagg
     9541 gcaagatttc cttcccttcc aagatgtgga agacagtgtc cgcaaggata tggctaagct
     9601 aagagcctcc tctcttatcc ctgatgatgt cgttattaac ggtgccgtct acgatgtgga
     9661 tactggaaag atgacacaag taatggaata gttagtagcc aagttgcaga gctgaattca
     9721 gctctttttt atttttaaaa attattgaaa atattagaca aagaaatagg gaagcgttat
     9781 aataattgta tcaagttatt catacaaaca aggagagtaa gatggtcgac aatagaatga
     9841 gatttacgat tgaccaaaac atgcaatttc cactagttga gattgatctg gaacatgggg
     9901 gatcagttta cttacaacaa ggaagtatgg tttatcatac tgagaatgta accttgaata
     9961 ccaaattgaa tggtaaggga tctggtttag gaaaattagt tggagccatc ggccgttcca
    10021 tggtttctgg tgaatcaatg ttcattacac aagctatgtc aaatggagat ggtaaattag
    10081 cattagcacc caatacacca ggacagattg ttgctttaga actaggtgaa aaacaatatc
    10141 gtctaaatga tggtgccttt ctagccctag atggttcagc acagtacaaa atggaacgtc
    10201 aaaatattgg aaaagcactt tttggtggtc aaggcggact ttttgtcatg acaactgaag
    10261 gcctgggtac cttacttgct aactcgtttg gatctatcaa gaaaatcaca cttgatggtg
    10321 gcacaatgac tattgataat gcccatgttg tagcatggag cagagaactt gattatgata
    10381 ttcatttaga aaatggcttt atgcagtcaa ttggtacagg tgaaggagtt gttaatacct
    10441 ttagaggtca tggtgagatt tatatccaaa gtcttaacct tgagcaattt gctgggactc
    10501 ttaaacgtta tctaccaact agttcaaatt agtcaaaata acataaaatc agctttttag
    10561 ggctgatttt tttcgcctaa gcaagtaaaa tatgctataa taagaaagat tttagaagaa
    10621 gtctgaaaag gtcttggcaa gctcaaatca tcatttgttg cttgtttttc gtgccacagg
    10681 tttctgagtg atatatgtaa caggagaaga agcatgtcta aaccaattcg tgtgcgttat
    10741 gcaccaagtc caacagggct cttacatatc gggaatgccc gcacagccct atttaattac
    10801 ctttatgccc gtcgtcacgg aggtactttt atcattcgta ttgaagatac tgaccgtaaa
    10861 cgtcatgttg aagatggtga acgttctcaa cttgaaaacc taaaatggtt aggaatggat
    10921 tgggatgaaa gcccagaaac gcatgaaaat taccgccagt ctgaacgtct agcactttat
    10981 caacaatata ttgataaatt gttagctgaa ggaaaagcct ataagtctta cgtgactgag
    11041 gaagaattgg cagcagagcg tgaacgccaa gaagcagctg gcgaaacgcc tcgctatatc
    11101 aatgaattta ttggtatgtc agctgacgaa aaagctaagt acatttcaga gcgtgaagca
    11161 gcaggtatcg ttccaacagt ccgtcttgct gttaatgagt ccggtattta caaatggact
    11221 gacatggtta aaggcgatat cgagtttgaa ggtggcaaca tcggtggtga ctgggttatt
    11281 caaaagaaag atggctaccc aacttataac tttgccgttg ttgttgatga tcatgatatg
    11341 caaatttctc acgttatccg tggcgatgac catattgcca atacgccaaa acaattgatg
    11401 gtctatgaag ctcttggctg ggaagcgcca gagtttggtc atatgactct tatcatcaat
    11461 tctgaaacgg gtaaaaaatt atcaaaacgc gacaccaata ccctccaatt cattgaagat
    11521 taccgcaaaa aaggttatat gcctgaagct gtctttaact tcatcgccct tcttggctgg
    11581 aatcctggcg gagaagagga aatcttctct cgtgagcaac tgattgccct ttttgatgaa
    11641 aatcgcctta gcaagtcgcc agctgccttt gaccagaaga aaatggattg gatgagcaat
    11701 gagtatctta aacatgcgga ttttgaaaca gtttatgccc tctgcaaacc tttccttgaa
    11761 gaggcaggcc gtttgacaga aaaagctgag aagctagtag aattatacaa acctcaatta
    11821 aaatctgcag atgagattat tccattgact gaccttttct tttctgattt ccctgaatta
    11881 acagaagccg aaaaagaagt gatggcaggt gaaacagttt cgacagtctt gcaagctttc
    11941 aaggctaagt tggaagctat gtcagatgaa gacttcaaac cagaaaatat tttcccacaa
    12001 attaaggctg ttcaaaaaga aacaggaatc aaagggaaaa acctctttat gcctattcgt
    12061 attgcagtat ctggtgaaat gcatggccca gaattaccaa atacgattta tttattggga
    12121 cgtgataaat ccattgagca catcaaaaac atgctataag gctgtatcgt tcacctttat
    12181 ttttaaacgc tttaaggagt taaaaagtca cgacaacgaa agattgaaga ttaagttctt
    12241 cagtcttttt tatcgttatc tgagcttgct accttggtaa agtaaaaaat agtatagttt
    12301 atttctattt tggagattgg atttgaaaga gaggtggtga gatgcttacg tgataatgac
    12361 agcaaacggt gaggttttga ttagtgtgat ttaaagaaat ggtttttatg aatcaattag
    12421 tgtacaatat ttttttggta tatgctgaaa gagactagtt tagtattcac ttagctaatc
    12481 aattgtaatc aaataatatt gtaatcattg tcataaaata tgctaaaata ataaagttgg
    12541 aaaggagaaa aaatggctaa tagcgaatat atgtttttat tattatcaat tattgtctat
    12601 tatatgacaa aaatttatat tttttccttt ctatctgata tcacattacc agtttggaaa
    12661 caactaacga ttttggcttt agccctattt ttcaatcagt tcccgtattt gtcgccattg
    12721 ctaattgatc ctctattatt tttagttgtt ttaaggcaag aaaccaagca attattttct
    12781 ttgaaagctc tttttttggc tgttgcacct agtgttttgg tggatttgct atctcgattt
    12841 atgggtacaa tcgttatccc ctatcttttt ttgtcgagtg gtatttatct gggtcatatt
    12901 atctttgact tgcttgctta tctgctgatt tttccaagtt ttgctattat taattatatg
    12961 attggaaaag attacaaggt gatatgccaa tccggatatt cgaaacgatc gcataatttc
    13021 tatcaaacat tgctgatttt tgttttggtt tattatgtgg acatttttgt cattttagga
    13081 tttacggacc cctttttaca ttttcatcat tctctttttg ttcccacacc ttataagtta
    13141 ttgtttttga tgtttatact tcttttagtt tatcttctat cttattttaa tcatagttct
    13201 aaagaatacc ttaaaaatga gttgagaaga gaacaacaag cttatatgac taacttagag
    13261 acttatggga aacatcttga gaaactctat cgagatgtaa gagcttttca aagtgattac
    13321 ctgagccgta tagagcgttt aggtcaagcc attaagagtg aatccattac tcagattcaa
    13381 gatatttatg cccaaacggt tcatgaagct aacgactact gggatgataa acactataat
    13441 atctcgaagc ttaggaaaat taatatctca tcgattaaga gcttattgtc agctaaaatt
    13501 atcagtgcgg aaaaatcagg tattgatttg aatgtggaag tgccagataa tataaaagag
    13561 acttacattc ctgagttgga cttactattg ttgatgtcta ttttttgtga taacgccatt
    13621 gaggcagctc ttgaagcaca gcaacctcat atgtcaattg cttacttttt gttagatgac
    13681 tatcagatgt ttgttgtgac aaatacgact aaaaaaaagg tagacattaa caagatcttt
    13741 gaagaaggct attcttcaaa aggttcagaa cgtggtatcg gcctttctaa tgcgcagcgt
    13801 attctcaaaa agtatcctta tttatccttg cggactaaaa gttttgataa ggaatttagc
    13861 caaaccttga ccatgccaaa ggaggaggtg gatagatgat ggttatatac agtatggctt
    13921 tattctatgc taccatcttt attaacagct gggtcatttt tgcaaaggtt tctgctatta
    13981 aactaagctg gaaacgggtt agcgttatag gcattagttt tgtaattgct aatatgatat
    14041 ttgacaaagt cattttgatt gaccaactgt tttttattat tgttagtttg ttaagtgctc
    14101 cgaaaaagaa attatttgag catatgttta atggtttctt tactatcttg attgtcgaat
    14161 tactgtttcg cgtcatcgga tctttctttt taccagctgt cttaggattt agcattggtc
    14221 aaattaataa taaccttaag cttttagagt tatgttatct ttttgtatta ccaatatttt
    14281 atctgtttag ctacattttc agtattgact taagcttaat aaggtttatc agtgaagata
    14341 agatgaaaaa gtgggtattt tggatgaata ctgccatgtt ttcttactac tttttcgccc
    14401 attttttggt aactgtgcaa agcggatttc ttgcactata tttccagtac cggtctattt
    14461 tagtatttat ttatttagct atttttattt gggttattgt gaaattagat cgatttgcta
    14521 aagatcagct gtcacaaaaa ttaacacaag cacaaaatga acgtattgcg tatcttgaaa
    14581 attacaatca atcgattgag caactttacc gagaaatcag aacagttaaa catgattctg
    14641 aaaatatttt aattagtcta aaagatagta tcgatagtgg tgatatagat ctgattacta
    14701 gagtttatga cacggttatt cagcaatcgg caacttctat gatgcgcaca aattatgaaa
    14761 tatcttcttt agacaatatc aaagagactg tgattcgcag catcatgaat tccaaattgc
    14821 ttgaagctca gtatttgggt attgaacttt atattgagat tccagatgtt atcgatcatt
    14881 tacctattaa gttaatcgac ttaattgtac tttttactgg actagtggac aatgctattg
    14941 agacagccaa aggcagtaga cgtccttttt tatctattgc atactttaaa caagataaca
    15001 agcaattatt tattattgaa aatagcacaa aaaccaatcg tgtggatata gccaaacgct
    15061 ttgatgctca gcagcagaac tcagcccact ttttgactgt tttagatagt tacccccaaa
    15121 taacgctttc gactaaaagt gaccattatc gattaagaca attgttagaa atgagataaa
    15181 gtatgatgaa catttttatt cttgaggatg attttatcca gcagacacgc atagaatcta
    15241 tcgtagtagg catcttaaaa gagactagaa taccatgtaa tcagcttgaa gtgttctcga
    15301 ctcctcaaaa attatttgag agtatccaag aaagaggaga ccatcagctg tattttttag
    15361 atattgaaat tggtgaatat actcgttgtg ggcttgaatt agctgcagct attcgacaaa
    15421 aagatcctaa tgccgttatt gtgtttgtca ccactcattc agaatttgct cctatttctt
    15481 ttaaatataa ggtatctgct ctagatttta ttgataaagc aggtggtcaa aaacagttta
    15541 aagaacaaat cgaagaatgt atccgatata cttacgatat gatgtctagt cgtgagtcaa
    15601 aggacatgtt tttatttgag actccacaga caagattaaa attgccttat aaggatattt
    15661 tatactttgc tacagccaca actcctcaca aagtttgttt atggactcag acagaaagat
    15721 tagagtttta tggtaatcta tctgagattc aagcagtagc accgaaactt tttttgtgcc
    15781 atagatccta ccttgtgaat ctagataagg ttgttcgaat tgataagagt aagcagctcc
    15841 tttatttcga aaatggtgat tcttgtatgg tatcacggtt aaaaatgaaa tgtctgtttg
    15901 aacggtggga ggcccttcgc taaagttaag agtgcaataa cattttagga cgaaaaagca
    15961 acatttcagg aaatgatgct cttattgaag tatttttcgc tataataatt aatgtaaata
    16021 aagatttacg aagtcatgag tttatcgagc aatcaatccc caattcaatc aaaatattgt
    16081 tttctctctc tccataaaca aatagtattt ttgatatgat ggctcggcag acaaaatgac
    16141 gatgtcagtt gtctttgttt ggatatcaca ttagctggtt gtaaaatcag tattaaagcc
    16201 cggcttgccg ggttttttga taggtaaata cagggataag tagctgacgt cttgtggatt
    16261 ctgacttttt atggttgttt tctgaaggtt attcctttgt cgaaagaaga actatactac
    16321 gaaaagatgg agtgacaaga ggcagttgaa gcacaaaaac tagaaatagt acagaaagag
    16381 gcttaatctg aaagggttag ggctttttaa ttttttaggg gatgtggtat aatggtaata
    16441 atttagatat tggagttaaa ttttgaagaa gacctatcgt gtcaagcgtg agaaagattt
    16501 ccaagccata tttaaggatg gaaaaagtac agcaaatcga aaatttgtca tttatcattt
    16561 aaatagaggc caagaccact tccgtgtggg tatttctgtc ggtaaaaaaa taggaaatgc
    16621 agtcaccaga aatgcagtca aacgaaagat acgtcatgtt atcatggcat tagggcatca
    16681 gctgaagtca gaggatttcg tggtgattgc ccgtaagggt gtcgagtctt tggagtatca
    16741 agagcttcaa caaaatttac atcatgtttt aaagttagca caattgcttg agaaaggttt
    16801 tgagagtgaa gaaaaacatt aaaattgcaa gaatagtccc cttagttcta ttattagtgg
    16861 cctgtggtcg tggtgaggta acggcacaat catctagtgg ttgggaccag ttggtttact
    16921 tatttgccag agcaattcaa tggctttcct ttgatggttc aattggtgtt ggcattattc
    16981 tttttaccct tactatccgt ctcatgctca tgcctttgtt taatatgcaa atcaaatcaa
    17041 gccagaagat gcaagacatc caacctgagc ttagagaatt gcaaaagaaa tacgctggta
    17101 aagacacaca aacgcggatg aagttggctg aagaaagtca agccctttac aaaaaatatg
    17161 gggttaaccc ttatgccagt ctcttacccc tcttaattca gatgccagtt atgattgccc
    17221 tattccaagc cttgacacgg gtatctttct taaaaacagg gactttcttg tgggtggaat
    17281 tggcacaaca tgatcatttg taccttttac cagttttggc agctgtcttc actttcttgt
    17341 ccacttggtt gaccaatcta gcggctaaag aaaaaaatgt catgatgact gttatgattt
    17401 atgtgatgcc gctaatgatc attttcatgg gctttaactt ggctagtgga gtagtgctct
    17461 attggacggt ctccaatgcc ttccaagtgt tacaactact gttattaaac aatcctttta
    17521 agattattgc cgaaagacag cgtcttgcca atgaagaaaa agaacgccgt cttcgtgaac
    17581 gtcgtgctcg taaaaaagca atgaagagaa aataggagtt taggttatgg tattatttac
    17641 aggaaaaaca gtcgaagaag ctatcgaaac aggacttcaa gagttagggt tatcacgcct
    17701 taaagcgcat atcaaagtca tttcaaaaga gaaaaaaggt ttcctcgggt ttggtaaaaa
    17761 gccagcccaa gttgatatcg aaggtatcag cgacaagacg gtttataagg ctgataaaaa
    17821 agccactcga ggtgttccag aggatattaa tcaaaaaaat gctcccgtgg tcaattctgc
    17881 tgatgtagaa cctgaagaaa taaaagccac tcaaaggtta gaagcagaag ataccaaagt
    17941 ggttcctctc atgtctgaag atagtccagc gcaaacccct tctaatcttg ctgagacagt
    18001 cactgaaaga aaagcacaac aaccgtcaat cccagtcgag gaatcagaag tgccccaaga
    18061 tcctggtaat gatggtttca gcaaagatat tgaaaaagct gctcaggaag tgtccgatta
    18121 tgtgactaaa atcatctatg aaatggatat cgaagccacc gttgaaacca gcaacaatcg
    18181 tcgccaaatc aatttgcaga ttgaaacgcc agaggcggga cgtgtgattg gttaccatgg
    18241 taaggtcttg aaatccttgc agttgttagc tcaaaatttc ttgcacgacc gctattcaaa
    18301 aaatttttca gtatccttga atgtccatga ttatgtggaa catcgaacag aaaccttgat
    18361 tgactttacg caaaaagttg ccaaacgcgt cttggaatct ggccaagatt acaccatgga
    18421 tcccatgagt aacagtgagc gtaaaatcgt tcataaaacc gtttcatcca ttgaaggggt
    18481 tgatagttat tctgaaggca atgaccctaa ccgctatgtg gtagtcagtc ttcaacgtta
    18541 gatcctaaaa agaatcgttg gtagttagtt tagctatcga agattttttg tatggccagc
    18601 ttaaccgttt gttttactga tctcattgat tattgttgct attgataagg tttcaacggt
    18661 aaggcaaagg aacagtgtca aagaagagac actttatgaa atgagcggcc tcttaaatga
    18721 gagagaaact gttgaaaaac gaaaataaat cttgacagta gaccctcttt aagatagaat
    18781 attatagtat gtttagtaac tgatatcaca aatagccggc taaacgaata caaaatctat
    18841 gaggaggtat tcactgtgaa acgtacttat caaccaagta aaatccgtcg tcaacgtaaa
    18901 cacggattcc gtcaccgtat gtcaactaaa aacggacgtc gtgtgcttgc tgcacgtcgt
    18961 cgtaaaggac gtaaagtatt atcagcttaa ttgcaaaaaa tacaactaaa gtaaaccaag
    19021 agtgctcgag gctcttggtt ttttaacgtt ttctaatcgg aaatttgcaa tgtataagta
    19081 tggtgtcaac tccttacctc cttatttgtt gtatgaaaga gcattccaaa taatagaaaa
    19141 taatataaaa aatgaa
//
LOCUS       DAUCLR010000030        18861 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.030, whole genome shotgun sequence.
ACCESSION   DAUCLR010000030 DAUCLR010000000
VERSION     DAUCLR010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18861
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.030"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            172..282
                     /locus_tag="VGT87_000886"
                     /pseudo
     CDS             172..282
                     /locus_tag="VGT87_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_129544913.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            534..2753
                     /locus_tag="VGT87_000887"
     CDS             534..2753
                     /locus_tag="VGT87_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEQ3813759.1"
                     /translation="MAKIMNVTGEEVIALAATYMTKADVAFVAKALAYATAAHFYQVR
                     KSGEPYIVHPIQVAGILADLHLDAVTVACGFLHDVVEDTDITLDEIEADFGQDARDIV
                     DGVTKLGKVEYKSHEEQLAENHRKMLMAMSKDIRVILVKLADRLHNMRTLKHLRKDKQ
                     ERISRETMEIYAPLAHRLGISRIKWELEDLSFRYLNETEFYKISHMMKEKRREREALV
                     EAIVSKVKTYTTQQGLFGDVYGRPKHIYSIYRKMRDKKKRFDQIFDLIAIRCVMETQS
                     DVYAMVGYIHELWRPMPGRFKDYIAAPKANGYQSIHTTVYGPKGPIEIQIRTKDMHQV
                     AEYGVAAHWAYKKGVRGKVNQAEQAVGMNWIKELVELQDASNGDAVDFVDSVKEDIFS
                     ERIYVFTPTGAVQELPKESGPIDFAYAIHTQIGEKATGAKVNGRMVPLTAKLKTGDVV
                     EIITNANSFGPSRDWVKLVKTNKARNKIRQFFKNQDKELSVNKGRDLLVSYFQEQGYV
                     ANKYLDKKRIEAILPKVSVKSEESLYAAVGFGDISPISVFNKLTEKERREEERAKAKA
                     EAEELVKGGEVKHENKDVLKVRSENGVIIQGASGLLMRIAKCCNPVPGDPIDGYITKG
                     RGIAIHRSDCHNIKSQDGYQERLIEVEWDLDNSSKDYQAEIDIYGLNRSGLLNDVLQI
                     LSNSTKSISTVNAQPTKDMKFANIHVSFGIPNLTHLTTVVEKIKAVPDVYSVKRTNG"
     gene            2768..3211
                     /locus_tag="VGT87_000888"
     CDS             2768..3211
                     /locus_tag="VGT87_000888"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEQ3813760.1"
                     /translation="MKLVLQRVKEASVSIDGKIAGAINQGLLLLVGVGPDDNAEDLAY
                     AVRKIVNMRIFSDADGKMNQSIQDIKGSILSVSQFTLYADTKKGNRPAFTGAAKPDLA
                     RQLYDSFNEQLAEFVPVERGVFGADMQVSLINDGPVTIILDTKCH"
     gene            complement(3309..4607)
                     /gene="ska"
                     /locus_tag="VGT87_000889"
     CDS             complement(3309..4607)
                     /gene="ska"
                     /locus_tag="VGT87_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023611186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptokinase"
                     /protein_id="HEQ3813761.1"
                     /translation="MKNYLSFGMFALLFALTFGTVKPVQAIAGYEWLLDRPSVNNSQL
                     VVSVAGTVEGTNQEISLKFFEIDLTSRPAHGGKTEQGLRPKSKMPYKLEKADLLKAIQ
                     EQLIANVHSNDGYFEVIDFASDATITDRNGKVYFADKDDSVTLPTQPVQEFLLSGHVR
                     VKPYQPKAVHNSAERVNVNYEVSFVSETGDLDFTPLLRNQYHLTTLAVGDSLSSQELA
                     AIAQFILSKKHPDYIITKRDSSIVTHDNDIFRTILPMDQEFTYHIKDREQAYKANSKT
                     GIEEKTNNTDLISEKYYVLKKGEKPYDPFDRSHLKLFTINYVDVNTNKLLKSEQLLTA
                     SERNLDFRDLYDPRDKAKLLYNNLDAFGIMDYTLTGKVEDNHDDTNRIITVYMGKRHE
                     GENASYHLAYDKDRYTEEEREVYSYLRYTGTPIPDNPKDK"
     gene            4947..5795
                     /locus_tag="VGT87_000890"
     CDS             4947..5795
                     /locus_tag="VGT87_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEQ3813762.1"
                     /translation="MELKDYFPEMQVGPHPLGDKEWVSVKEGDQYVHFPKSCLSEKER
                     LLLEVGLGQCEVLQPLGSPWQRYLLDHQGNPPQLYETSQFIYLNHQQALPADLVELLQ
                     QMIAGLEVILPISATQTAFLCRQAISIKVLRWLEDLLPTLESDFGLALTMFVGNAWYQ
                     VAAGTLRECFEEECQLLTAYLRQQSGRKLLTFSGLMLWSLLSHHTFLALTRQFHQFLS
                     PQSDMADVVHALWSEHGNLVQTAQRLYIHRNSLQYKLDKFAQQSGLHLKQLDDLAFAH
                     LFLLKY"
     gene            6093..7226
                     /gene="ugpC"
                     /locus_tag="VGT87_000891"
     CDS             6093..7226
                     /gene="ugpC"
                     /locus_tag="VGT87_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEQ3813763.1"
                     /translation="MVELNLNHIYKKYPNTTHYAVEDFDLDIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELKIGGEVVNDKSPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYKKDDIDRRVKEAAQILGLTEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGSTTIYVTHDQTEAMTLADRIVIMSATKNPQGN
                     GTIGKIEQVGSPQELYNLPANKFVAGFIGSPAMNFFEVEVKDGRIVSEDGLDIAIPEG
                     QAKMLEAAGYKGKKVTFGIRPEDISSRQIVHDTYPSATVTAEVLVSELLGSETMLYVK
                     LGQTEFASRVDARDFHSPGEQVSLTFNVAKGHFFDRDTEQAIR"
     gene            7355..8968
                     /locus_tag="VGT87_000892"
     CDS             7355..8968
                     /locus_tag="VGT87_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEQ3813764.1"
                     /translation="MKKHWWHKATIYQIYPKSFKDTNGNGIGDLKGITSQLDYLQKLG
                     ITAIWLSPVYQSPMDDNGYDISDYEAIADVFGDMADMDELLAAANERGIKIIMDLVVN
                     HTSDEHAWFVEARENPNSPERDFYIWRDEPNDLTSIFSGSAWEYDNASGQYYLHLFSK
                     RQPDLNWENEALRHKIYDMMNFWIAKGIGGFRMDVIDLIGKVPDSEITGNGPRLHDYL
                     KEMNQATFGNHDVMTVGETWGATPEIARQYSRPENKELSMVFQFEHVGLQHKPDAPKW
                     DYAKELDVPALKAIFSKWQTELKLGEGWNSLFWNNHDLPRVLSIWGNDSTYREKSAKA
                     LAILLHLMRGTPYIYQGEEIGMTNYPFKDLTEVDDIESLNYAKEAMGNGVSAARVMDS
                     IRKVGRDNARTPMQWSKDTHAGFSKAQETWLPVNPNYQDINVADALADPDSIFYTYQK
                     LIALRKEQDWLVEADYHLLPTADKVFAYQRQLGEETYVIVVNVSDEEQVFATDLAGAQ
                     VIIANTDVDTVLETKHLQPWDAFCLKLKA"
     gene            9166..12663
                     /locus_tag="VGT87_000893"
     CDS             9166..12663
                     /locus_tag="VGT87_000893"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase"
                     /protein_id="HEQ3813765.1"
                     /translation="MKKKVNQGSKRYQYLLKKRGIGFVIAATGTVMLGCTPSILTHQV
                     AAKTIVGLARDEAQQGDGNAKSGDGFQSSSKEAKPVLDSSSTNPDSIAEHHLRMHFKT
                     LPAGETLGSLGLWVWGDVDQPSKDWPNGAITMTKAKKDDYGYYLDVPLAAKHRQQVSY
                     LINNKAGENLSKDQHISLLTPKMNEVWIDENYHAHAYRPLKKGYLRINYHNQSGHYDN
                     LAVWTFKDVKTPTTDWPNGLDLSHKGPYGAYVDVPLKEGANEIGFLILDKSKTGDAIK
                     VQPKDYLFKELDNHTQVFVKDTDPKVYNNPYYIDQVSLKGAEQTTPNEIKAIFTTLDG
                     LDEDAVKQNIKITDKAGKTVAIDELTLDKDKSVMTLKGDFKAQGAVYTVTFGEVSQVA
                     RQSWQLKDKLYAYDGELGATLAKDGSVDLALWSPSADTVKVVVYDKQDQTKVLGQANL
                     TKSDKGVWRAHLTSDSIKGISDYTGYYYLYEITRGQEKVMVLDPYAKSLAAWNNATAT
                     DDIKTAKAAFIDPSKLGPAGLDFAKINNFKKREDAIIYEAHVRDFTSDKALEGKLTHP
                     FGTFSAFVEQLDYLKDLGVTHVQLLPVLSYFYANELDKSRSTAYTSSDNNYNWGYDPQ
                     HYFALSGMYSANPNDPALRIAELKNLVNEIHKRGMGVIFDVVYNHTARTYLFEDLEPN
                     YYHFMNADGTARESFGGGRLGTTHAMSRRILVDSITYLTREFKVDGFRFDMMGDHDAA
                     AIEQAFKAAKAINPNTIMIGEGWRTYQGDEGKKEIAADQDWMKATNTVGVFSDDIRNT
                     LKSGFPNEGTAAFITGGAKNLEGLFKTIKAQPSNFEADAPGDVVQYIAAHDNLTLHDV
                     IAKSINKDPKVAEEEIHKRIRLGNTMILTAQGTAFIHSGQEYGRTKQLLNPDYKTKVS
                     DDKVPNKATLIDAVAQYPYFIHDSYDSSDAVNHFDWAKATDSIAHPISNQTKAYTQGL
                     IALRRSTDAFTKATKAEVDRDVTLITQAGQDGIQQEDLIMGYQTVASNGDRYAVFVNA
                     DNKTRKVVLPQAYRYLLRAQVLADAEQAGVTAIAKPKGVQFTKEGLTIDGLTALVLKV
                     SSKTANPSQQKSQTDNHQTKTPDGSKDLDKSLMTRPKRAKTNQKLPKTGEAPSKGLLA
                     AGVALLLLAISLLMKRQKD"
     gene            12778..13152
                     /locus_tag="VGT87_000894"
     CDS             12778..13152
                     /locus_tag="VGT87_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HEQ3813766.1"
                     /translation="MRPKEKETSMTFEEIVANFIPSSVAEVTSAIASGKDMIVFLGRS
                     SCPYCRRFAPKLAQVATDNQKEVYFVDSENAADAAELAAFRENYQLVTVPALLVSYDQ
                     HQRAVCDSSLTPDDILAFLTRE"
     gene            13268..13633
                     /gene="yajC"
                     /locus_tag="VGT87_000895"
     CDS             13268..13633
                     /gene="yajC"
                     /locus_tag="VGT87_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEQ3813767.1"
                     /translation="MGFPTILMFVVMLGLIWFMQRQQKKQAQERQNQLNAIEKGDEVV
                     TIGGMFAIVDEVDTTAKKIVLDVDGVFLTFELSAIKRIVTKATTETTLVETEETSVVN
                     EPAVETVDSTDTDSAIESH"
     gene            13852..14601
                     /locus_tag="VGT87_000896"
     CDS             13852..14601
                     /locus_tag="VGT87_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEQ3813768.1"
                     /translation="MFGLKAKSTKKVLGSIPKHIGIIMDGNGRWAKKRLKPRVFGHKA
                     GMDALQEVTITASELGVKVLTVYAFSTENWSRPQDEVSFIMNLPVTFFDKYVPVLHEN
                     NVKIQMIGETSRLPEDTLAALNAAIDKTKRNTGLILNFALNYGGRAEITSAVRFIAQD
                     VLDAKLNPGDITEDLIANYLMTDHLPYLYRDPDLIIRTSGELRLSNFLPWQSAYSEFY
                     FTPVLWPDFKKAELLKAIADYNRRQRRFGKV"
     gene            14614..15408
                     /locus_tag="VGT87_000897"
     CDS             14614..15408
                     /locus_tag="VGT87_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002275264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEQ3813769.1"
                     /translation="MKERVVWGGVAVAIFLPFLIIGNLPFQLFVGVLAMIGVSELLKM
                     KRLEVFSFEGVFAMLAAFVLAVPMDHYLTFLPIDANVAFYSLMVFFILAGTVLNSRAY
                     SFDDAAFPIATSFYVGIGFQHLINARLSGIDKVFLALFIVWATDIGAYLIGRQFGRRK
                     LLPRVSPNKTIEGSLGGIACAVLVSFIFMVIDRSVYAPHHFLTMLVLVALFSIFAQFG
                     DLVESALKRHFGVKDSGKLIPGHGGILDRFDSMIFVFPIMHLFGLF"
     gene            15481..16740
                     /gene="rseP"
                     /locus_tag="VGT87_000898"
     CDS             15481..16740
                     /gene="rseP"
                     /locus_tag="VGT87_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEQ3813770.1"
                     /translation="MLGIITFIIIFGILVIVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FSHVDQGGTLYTLRMLPLGGYVRMAGWGDDKTEIKTGTPASLTLNEQGFVKRINLSQS
                     KLDPTSLPMHVTGYDLEDQLSITGLVLEETKTYKVAHDATIVEEDGTEIRIAPLDVQY
                     QNASIGGRLITNFAGPMNNFILGIVVFILLVFLQGGMPDFSSNHVRVQENGAAAKAGL
                     RDNDQIVAINGYKVTSWNDLTEAVDLATRDLGPSQTIKVTYKSHQRLKTVAVKPQKHA
                     KTYTIGVKASLKTGFKDKLLGGLELAWSGAFTILNALKGLITGFSLNKLGGPVAMYDM
                     SNQAAQNGLESVLYLMAMLSINLGIFNLIPIPALDGGKILMNIIEAIRRKPIKQETEA
                     YITLAGVAIMVVLMIAVTWNDIMRVFF"
     gene            16938..18794
                     /locus_tag="VGT87_000899"
     CDS             16938..18794
                     /locus_tag="VGT87_000899"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEQ3813771.1"
                     /translation="MKQSKLLIPTLREMPSDAQVISHALMVRAGYVRQVSAGIYAYLP
                     LANRTIEKFKTIMREEFEKIGAVEMLAPALLTADLWRESGRYETYGEDLYKLKNRDNS
                     DFILGPTHEETFTTLVRDAVKSYKQLPLNLYQIQSKYRDEKRPRNGLLRTREFIMKDG
                     YSFHHNYEDLDVTYEDYRQAYEAIFTRAGLDFKGIIGDGGAMGGKDSQEFMAITPART
                     DLDRWVVLDKSVASMDDIPKEVLEEIKAELAAWMISGEDTIAYSTESSYAANLEMATN
                     EYKPSSKVAAEDALAEVETPHCKTIDEVAAFLSVDETQTIKTLLFVADNEPVVALLVG
                     NDHINTVKLKNYLAADFLEPASEEEARAFFGAGFGSLGPVNLAQGSRIVADRKVQNLT
                     NAVAGANKDGFHVTGVNPGRDFQAEYVDIREVKEGEMSPDGHGVLQFARGIEVGHIFK
                     LGTRYSDSMGATILDENGRAVPIVMGCYGIGVSRILSAVIEQHARLFVNKTPKGDYRY
                     AWGINFPKELAPFDVHLITVNVKDQAAQDLTAKLEADLMAKGYDVLTDDRNERVGSKF
                     SDSDLIGLPIRVTVGKKAAEGIVEIKIKATGDSIEVNAENLIETLEILTKEH"
BASE COUNT         5572 a         3621 c         4313 g         5355 t
ORIGIN      
        1 ccagaagtga caccaacacc agaaacgcca gagcaaccag gcgaaaaagc tccagaaaag
       61 agcaaagagg taactccagc tccagaaaaa cctgctgaca aagaagctaa ccaaacgcca
      121 gaacgtcgca atggcaatat ggctaagaca cctgtagcca acaaccacag acgtctacca
      181 gcaactggtg agcaagccaa cccattcttt acagcagcag cagtagcagt gatgacaaca
      241 gctggtgtcc tagccgttac aaaacgtaaa gaaaacaact aatcctctaa attgagaggc
      301 cttactgata agcaaacata atagagagag ccccttggtt ctctctattt ttggtcggga
      361 gcaggccttt ggtatcatgc tcacgcctga gagatcccaa aagatatcaa aagcagctca
      421 ctaaccgttt tctaagaaaa ggtcacacta agcgctttct tagcagattc tgccttttgc
      481 ttttgtgtgt aaagtgttaa aatagggaaa agaaatgcta gatgaggaac gaaatggcaa
      541 aaataatgaa cgtaacagga gaagaagtca ttgccttagc ggccacctat atgaccaagg
      601 ctgatgtggc ttttgtggca aaggctttag catatgcaac agcggcccat ttctaccaag
      661 tgagaaagtc aggcgaacct tatatcgtcc atccgattca ggtggcgggg attctggctg
      721 atttgcatct ggatgctgtg acagttgcct gtggcttttt acatgatgtc gtagaagata
      781 cggatattac cttagatgag atcgaagcag actttggcca agatgctcgt gatatcgttg
      841 atggtgtcac caagttaggt aaagttgagt acaaatctca tgaggagcaa ctcgccgaaa
      901 accatcgcaa aatgctgatg gctatgtcca aagatattcg cgtgattttg gtgaaattgg
      961 ctgaccgcct gcataatatg cgcaccctca aacatttgcg caaggacaaa caagagcgta
     1021 tttcgcgcga aaccatggaa atctatgccc ccttggcgca tcgtttgggg attagtcgca
     1081 tcaaatggga actagaagat ttgtcttttc gttacctcaa tgaaaccgaa ttttacaaaa
     1141 tttcccatat gatgaaagaa aaacgtcgcg agcgtgaagc tttggtagag gctattgtca
     1201 gtaaggtcaa aacctatacg acacaacaag gcttgtttgg agatgtgtat ggtcgaccaa
     1261 aacacattta ttccatttat cggaaaatgc gggacaaaaa gaaacgtttc gatcagattt
     1321 ttgatttgat tgctatccgt tgtgtcatgg aaacgcagag cgatgtttat gctatggttg
     1381 gctatattca tgagctttgg cgtcccatgc caggccgctt caaggattat attgcagctc
     1441 ctaaagctaa tggctaccag tcgattcata ccaccgtgta tggaccaaaa ggacctattg
     1501 agattcaaat cagaactaag gacatgcatc aagtggctga gtacggggtt gctgctcact
     1561 gggcttataa aaaaggcgtg cgtggtaagg tcaatcaggc tgagcaagcc gttggcatga
     1621 actggatcaa agagctggta gaattgcaag atgcctcaaa tggtgatgca gtggactttg
     1681 tggattcggt caaagaagac attttttctg aacggattta tgtctttaca ccgacagggg
     1741 ccgttcagga gttgccaaaa gaatcaggtc ctattgattt tgcttatgcg atccatacgc
     1801 aaatcggtga aaaagcaaca ggtgccaaag tcaatggacg tatggttcct ctcactgcca
     1861 agttaaaaac aggcgatgtg gttgaaatca tcaccaatgc caattccttt ggccctagtc
     1921 gagactgggt aaaactggtc aaaaccaata aggctcgcaa caaaattcgt cagttcttta
     1981 aaaatcaaga caaggaattg tcagtgaata aaggccgtga tttgttggtg tcttattttc
     2041 aagagcaggg ctacgttgcc aataaatacc ttgacaaaaa acgcattgaa gcgatccttc
     2101 caaaagtcag tgtgaagagc gaagaatcgc tctatgcagc cgttgggttt ggtgacatta
     2161 gtcctatcag tgtctttaac aagttaaccg aaaaagagcg ccgtgaagaa gaaagggcca
     2221 aggctaaagc agaagctgaa gaattggtta agggcggtga ggtcaaacac gaaaacaaag
     2281 atgtgctcaa ggttcgcagt gaaaatggag tcattatcca aggagcatca ggcctcttga
     2341 tgcggattgc caagtgttgt aatcctgtac ctggtgatcc tattgacggc tacattacca
     2401 aagggcgtgg cattgcgatt cacagatcgg actgtcataa cattaagagt caagatggct
     2461 accaagaacg cttgattgag gtcgagtggg atttggacaa ttcgagtaaa gattatcagg
     2521 ctgaaattga catctatggg ctcaatcgta gtggcctgct caatgatgtg ctccaaattt
     2581 tatcaaactc aaccaagagc atatcgacag tcaatgctca gccgaccaaa gacatgaagt
     2641 ttgctaatat tcacgtgagc tttggcattc caaatctgac gcatctgacc actgttgtcg
     2701 aaaaaatcaa ggcagttcca gatgtttata gtgtgaagcg gaccaatggc taagaaagga
     2761 taaaaacatg aaacttgtct tgcaacgggt caaagaggcc tctgtttcta ttgatgggaa
     2821 aattgctggc gctattaatc aaggattgtt gttgttagta ggtgtgggac cagatgataa
     2881 tgcagaagat ttggcttacg ctgtgcgaaa aatagtcaac atgcgcattt tttcagatgc
     2941 tgatggcaag atgaatcagt ctatccaaga catcaagggc agtatcttat ctgtgtcgca
     3001 atttaccctt tatgctgaca ccaaaaaggg aaaccgtcct gcctttactg gtgctgctaa
     3061 gccagatttg gctaggcagt tgtatgatag ctttaatgaa caattggcag agtttgtgcc
     3121 agttgagcgt ggcgtttttg gggcagatat gcaggttagt ctcatcaatg atggaccagt
     3181 tacgattatt ttagacacga aatgtcacta gtctctacaa ctaagagatt agaaaaaagc
     3241 aagcaactgt tgatagcgtg ttgcttgctt tttttgtcag ttaatctcat cgttttagaa
     3301 gatcgtggtt atttgtcttt agggttatca ggtataggtg tccctgtata acgcaggtag
     3361 ctgtaaactt ctcgttcttc ttcggtataa cgatctttat cataagctaa atgatagcta
     3421 gcattctctc cttcgtgtcg cttgcccata taaacggtta tgatacggtt ggtgtcatcg
     3481 tgattatcct cgacttttcc agttaaggta tagtccataa taccaaaagc atcgagattg
     3541 ttgtagagta gtttagcctt atcacgagga tcgtataaat ctctgaagtc taagttacgt
     3601 tcgctagctg ttaagagctg ctcgcttttt agcaatttgt tggtgttgac atcaacgtaa
     3661 ttgatggtga acagtttcaa gtgactgcga tcaaagggat catacggctt ttcccctttt
     3721 ttaaggacgt aatatttctc agaaataagg tcagtgttgt tcgttttttc ttcaatacct
     3781 gttttagaat tggccttata agcttgttcc cggtctttga tatggtaagt aaactcttga
     3841 tccattggta aaatcgtacg gaaaatgtca ttgtcatgag tgacgattga ggagtcacgt
     3901 tttgtaatga tataatctgg atgttttttt gataggataa attgggcaat tgctgctaac
     3961 tcttgtgatg aaagagagtc accaactgcc agtgtggtca aatggtattg gtttcttaac
     4021 aacggtgtaa agtctaaatc tcctgtttcg gagacaaagc tcacttcata gttgacgtta
     4081 acgcgttcag cagagttgtg aacggcttta ggttgatacg gtttaacgcg cacatgccca
     4141 cttagcaaaa attcttggac aggttgggtc ggcaaggtta ccgaatcatc tttgtcagca
     4201 aagtagacct tgccgtttcg atcagtaatg gttgcatcgc ttgcaaaatc aatgacctca
     4261 aagtagccgt cgttactgtg aacgttagcg atcaattgtt cttgaatagc ctttaataag
     4321 tcagcttttt ccagtttata tggcattttt gattttggac gtaagccttg ctctgtcttt
     4381 cctccatgag caggtcgtga tgttaggtca atttcaaaaa atttaaggct aatttcttga
     4441 ttcgtcccct caacagtacc agcaacgcta acaactaatt ggctgttgtt gacagatgga
     4501 cggtctagca gccattcata cccagcaata gcttggacag gcttgactgt tccaaatgtt
     4561 aatgcaaaca gcagtgcaaa catcccaaaa gataagtaat ttttcataga aacctcctaa
     4621 aagttaagtt tcaatcccca ttattagaat aacataataa ttttatagat acaaacaaaa
     4681 acctaataat tgtttttaaa tgatatctta atgataatgt catgataatg aaaaaagaga
     4741 aacaatacct attttagtat aaaaataaat taatcttccg ataaatagct atttgattga
     4801 atgttcttgc taaaaatgaa taaccattat ttagcttcaa tgattagaaa gtcagaaaat
     4861 acaaaatcat gctgacaaca aaccgaactg tgtgataaag agtcatgtgt tataatggtt
     4921 taaagaccgt catgaaagga atggctatgg agctaaaaga ctattttcca gagatgcaag
     4981 taggccctca tccgttagga gataaagagt gggtgagtgt caaagaaggg gaccaatatg
     5041 tgcattttcc caaatcatgt ttatccgaaa aagagcgctt attacttgag gtgggactgg
     5101 gtcagtgtga ggtcttgcag ccgctagggt caccttggca gcggtatttg cttgatcatc
     5161 agggcaatcc ccctcagctg tatgagactt ctcagttcat ttacctcaat catcagcagg
     5221 ctttaccagc tgacttagtt gagttgctcc aacagatgat tgcagggctt gaggtcattt
     5281 tgcctatcag cgcaactcag acggcatttt tatgccgaca agcaatctca ataaaggtct
     5341 tgcgctggct tgaagatctc ctgccaacgc tagaaagtga ttttggtttg gccttaacca
     5401 tgtttgttgg aaatgcctgg tatcaagtag ctgcgggtac attgagagag tgttttgagg
     5461 aggaatgtca gctgttaacg gcctatttaa ggcaacaatc aggcagaaaa ttattaacat
     5521 tttcaggact gatgctgtgg tctttgctgt cgcaccacac ttttttggca ttgacgcgtc
     5581 agtttcacca attcctaagt cctcaaagtg atatggccga tgtggttcac gcgctatggt
     5641 cagaacacgg caatctggtg cagacagcgc agcgcctcta cattcatcgc aattcgttgc
     5701 aatataagct tgataaattt gctcagcaat caggactaca cttgaagcaa ttggatgatt
     5761 tagcctttgc tcatctcttt ttgttgaaat attaggaaag ccacagggct ttcttttttg
     5821 ctgccttgac tgtagctgat aaggaggatg atagatcgtt cagcttgttt tggctcctgt
     5881 gaccggcctg tgtttacatg gttttcttgt tttatgggtc tttacgactt gactagccct
     5941 gctattgtgc aagttgccca aagaagtttg gtcgctttgt ctctgttttg aaagcgctat
     6001 ttttgttata ataaaagcat catataaaag atatcagcgg tttctagctt tgcagtcagc
     6061 tagaacgttt gctattcaaa taggagttac acatggttga attaaatcta aatcacatct
     6121 ataaaaaata ccccaatact actcattatg ccgttgaaga ttttgacttg gacatcaaag
     6181 ataaagaatt tatcgtcttt gttggacctt caggatgtgg taaatcgact actcttcgta
     6241 tgattgcagg tcttgaggac atttctgaag gagagttaaa aatcggtggc gaagtggtga
     6301 atgacaagtc tcctaaagac cgtgatattg ccatggtctt tcaaaactac gccctttacc
     6361 cacacatgac cgtgtatgat aacatggcct ttggtttgaa attacgtaag tacaaaaaag
     6421 atgatattga ccgtcgtgtc aaggaagctg cgcagatcct tggtttgacc gaatttttag
     6481 agcgtaaacc tgctgacctg tctggtggac aacgccaacg tgttgccatg gggcgtgcta
     6541 ttgtccgtga tgctaaggta ttcttgatgg atgaaccttt gtcaaacttg gatgcgaagc
     6601 ttcgtgtctc catgcgtgct gaaatcgcaa agattcatcg taggattggc tcaacaacta
     6661 tttacgttac ccatgaccag acagaagcca tgaccttggc agaccgtatc gttatcatga
     6721 gtgccactaa aaaccctcaa ggtaatggaa cgattggtaa aattgagcaa gtaggttcac
     6781 cacaagaatt gtacaacctt ccagctaata aattcgttgc cggttttatc ggaagccctg
     6841 ccatgaactt ctttgaagtg gaagttaaag atggccgcat tgtgagtgaa gatggtcttg
     6901 acattgctat tcctgaaggt caagcaaaaa tgcttgaagc tgctggttac aaggggaaaa
     6961 aagtcacttt tggtattcgt ccagaagaca tttctagtag acaaatcgtg catgatactt
     7021 atccaagtgc aactgtcaca gcagaagttt tggtttctga gttactggga tctgagacga
     7081 tgctctatgt caagcttgga caaactgagt ttgcttctcg ggtagatgcg cgtgatttcc
     7141 atagtccagg ggagcaagtt agcttgacct ttaatgtggc taaggggcat ttctttgaca
     7201 gagataccga acaagctatt cgttaaggta taagcgatat tcataaaaag gttgggttcc
     7261 agccttttta tcatgtcatt tgcgcaaacg attgcacaaa aggagaaaaa acgttaaaat
     7321 aaaagatgaa tgaatgatta ataggagaag aataatgaag aaacactggt ggcataaagc
     7381 tacgatttat cagatttacc cgaaatcctt taaagacacc aatggcaatg gtattggtga
     7441 tttaaaaggc attaccagtc agctggacta cttgcaaaaa cttggcatta cagccatttg
     7501 gttatctcct gtttaccagt cgcccatgga tgacaatggc tatgacatct ctgactatga
     7561 agcgattgct gatgtttttg gagacatggc agatatggat gagctcttag cagcagctaa
     7621 tgagcgaggc attaagatta tcatggactt ggtggtcaac catacgtctg atgagcatgc
     7681 ttggtttgtt gaagctaggg aaaaccctaa tagtcctgag cgtgattttt atatttggcg
     7741 agatgaacct aacgatttga cttctatttt tagtggctca gcttgggaat atgataatgc
     7801 atcaggtcaa tattatttgc acctttttag caaacgtcag ccagatctca actgggagaa
     7861 tgaagcttta cgtcataaaa tttacgatat gatgaatttc tggattgcta aaggcatcgg
     7921 tggttttcgt atggacgtga ttgacctgat tgggaaggtt ccagattctg aaatcacggg
     7981 taacgggccg cgcttacacg attacctcaa agaaatgaat caagcaacct ttggcaacca
     8041 tgacgtgatg actgtcggtg agacttgggg agcaacgcct gaaattgctc gccagtattc
     8101 tcgccctgaa aacaaagagt tatccatggt cttccaattt gagcatgtgg gtcttcaaca
     8161 taaacctgac gctcccaagt gggattatgc caaagaatta gacgtgcctg ctctgaaagc
     8221 tattttcagc aagtggcaga cagaattaaa gttgggagaa ggctggaatt cgcttttttg
     8281 gaataaccat gacttgccac gggtcttatc catttggggc aatgacagca cttaccgaga
     8341 aaaatcagcc aaggctttag ctattttact gcatttgatg cgaggaaccc cttacattta
     8401 ccaaggagaa gagattggca tgaccaacta tccttttaaa gacttaactg aggttgatga
     8461 tattgagtca ctcaactatg ctaaggaagc tatgggaaat ggagtgtcag ctgcgcgtgt
     8521 gatggatagc atccgtaagg taggacgaga caatgctaga acgccgatgc agtggtctaa
     8581 ggacacacat gctggctttt ctaaagccca agagacttgg ttgccggtca accccaacta
     8641 ccaagacatc aatgtggccg atgcccttgc tgatccagac tctatttttt acacctacca
     8701 aaaattgatt gccttgcgta aggagcaaga ctggctagtt gaagcggatt atcacctctt
     8761 gccaacagct gataaagtgt ttgcttatca gcgtcagctt ggagaagaga cgtatgtgat
     8821 tgttgtcaat gtgtctgatg aggagcaagt ctttgccaca gacttagcag gtgcacaagt
     8881 gatcatcgcc aatacagatg tggacacggt attagagacc aagcacttac aaccttggga
     8941 tgccttttgt ctcaaactga aggcctagca gttgtatgtg atttgagttt acccaacagc
     9001 caagcgagcg gaaactcgtt ttggctgttt gattcttgag atcctattaa ggggtgatag
     9061 tcattgtttg agctgtgttc taagttgaag tgcggcacgt cttgcaagcg cttgcgcaat
     9121 gtgataagat agtcttgttt gaatgttata ctaaatggag gtcttatgaa aaagaaagtc
     9181 aaccaaggat caaagcgcta tcagtatctg ttaaaaaagc gggggatagg ttttgtaatc
     9241 gctgcaactg ggactgtcat gttagggtgc acccctagta tcttaacaca tcaagttgct
     9301 gctaaaacca ttgttggact agctcgcgat gaagctcaac aaggagatgg caatgctaaa
     9361 tctggtgatg gttttcaatc gtctagcaag gaggcaaaac cagttttaga cagctcgtca
     9421 actaatcctg atagtattgc tgagcatcat ttgcgtatgc attttaaaac attgccagct
     9481 ggtgagacgc taggaagctt gggactttgg gtgtggggag atgtggatca accttcaaag
     9541 gattggccaa atggtgctat caccatgaca aaggcgaaaa aagatgacta tggctattat
     9601 ctagatgtgc cactagcagc taaacaccgc cagcaagtgt cttatctcat taataataaa
     9661 gctggagaga atctttcaaa ggaccagcac atctcgcttc tcacgccaaa aatgaatgaa
     9721 gtttggatag acgagaatta ccatgcgcac gcttatcgac ctttgaaaaa aggttacctt
     9781 cgaatcaact accacaatca atcgggacac tacgataact tagctgtctg gacctttaaa
     9841 gatgtcaaaa ccccaacgac cgactggcca aatggacttg acttgtcaca taaagggcct
     9901 tatggagctt atgttgatgt ccccttaaaa gaaggagcta acgaaatcgg atttttaatc
     9961 cttgataaaa gtaagacagg agatgctatt aaagtgcaac caaaagatta tctgtttaaa
    10021 gagttagaca atcatactca ggtttttgtc aaagacactg acccaaaagt ttacaacaat
    10081 ccttattata ttgatcaggt tagtctcaaa ggagctgaac aaaccacgcc aaatgagatt
    10141 aaagccattt ttacgacctt agatgggctt gatgaagatg cggtgaaaca aaacatcaag
    10201 atcactgaca aagcagggaa aactgttgca attgatgagt tgacacttga caaggataag
    10261 tctgtaatga cattaaaggg tgattttaag gcacaaggtg cagtctacac ggttacattt
    10321 ggagaagtta gccaagtcgc tcgccaatcc tggcaattaa aagataaact ctatgcttac
    10381 gatggtgaac ttggagctac cctagctaag gatggttctg ttgatttagc gctatggtct
    10441 ccaagtgctg atactgttaa ggttgtcgtt tatgataaac aagatcagac aaaggttctt
    10501 ggtcaagcta atttgaccaa gtcggacaag ggtgtttgga gagctcatct gacttctgat
    10561 agtatcaagg gcattagtga ttacacaggc tactattacc tttatgaaat cacgcgcggt
    10621 caggaaaaag tcatggtttt ggatccttac gccaaatctc tcgctgcctg gaataatgcg
    10681 actgctactg atgacatcaa aacagcaaaa gctgccttta ttgatccaag caaactagga
    10741 ccagcaggcc ttgattttgc caaaattaac aactttaaaa agcgtgaaga cgctattatc
    10801 tatgaagcac atgtgcgaga ttttacgtca gataaggctc tagaaggcaa gttaacacac
    10861 ccttttggga ctttttcagc tttcgttgaa cagctagact atctcaaaga cttgggggtt
    10921 acccacgttc aattgctacc ggttttgagt tatttttatg ccaatgagct ggacaagagc
    10981 cgctcaacag cctacacgtc ttcagataat aattacaact ggggttatga cccacaacac
    11041 tactttgccc tttctggcat gtattcggca aatcctaacg accctgcttt acgtatcgca
    11101 gagcttaaaa accttgtcaa tgagattcac aaacgtggta tgggtgttat ttttgatgtg
    11161 gtttataacc acacggctag aacctatctc tttgaagatt tggaacccaa ctactatcat
    11221 tttatgaatg ctgatggcac agctagagag agttttggcg gaggtcgtct aggaacgaca
    11281 catgccatga gccgtcgtat cttggtggat tcgattactt atctgactcg tgaattcaag
    11341 gtagatggtt ttcgtttcga catgatgggt gaccatgatg cggcagctat tgagcaagcc
    11401 tttaaggcag ccaaagccat taatccaaat accattatga ttggcgaagg ctggcgtacc
    11461 taccaaggtg atgaggggaa aaaagaaatt gcggcagatc aagattggat gaaagcaacc
    11521 aatacggtcg gtgttttctc tgatgatatc agaaataccc tcaaatcagg ttttccaaat
    11581 gaaggcacag cagcctttat tactggtggc gcaaaaaatc tagaaggact gttcaaaacg
    11641 atcaaagcac agcctagtaa ttttgaagca gatgcccctg gagatgtagt gcagtatatt
    11701 gcagcccatg acaacctgac cttacatgat gtcattgcca aatccatcaa taaggatcct
    11761 aaagtggctg aagaagagat tcacaagcgt attcgtctag gaaataccat gattttaact
    11821 gctcaaggga ctgcctttat ccattctggt caggaatatg gacgaaccaa gcagcttcta
    11881 aatcccgact acaagacaaa ggtgtctgat gacaaggtgc caaataaggc gactctgatt
    11941 gatgctgtag cgcaataccc ttacttcatc cacgattctt atgattcgtc tgatgcggtc
    12001 aatcattttg actgggcaaa ggcaacagat tccatagctc acccgattag caaccaaaca
    12061 aaagcctata cacagggact aattgcgttg cgtcgctcaa cagatgcctt tacaaaagca
    12121 accaaagctg aggtagatcg ggatgtgacc ttgatcaccc aagcaggaca agatggtatt
    12181 caacaagagg acctcatcat gggttaccaa acggtggcat caaatggaga tcgctatgct
    12241 gtctttgtca atgcagacaa caagacccgc aaggtagttt tacctcaagc ctaccgctat
    12301 ttgctaagag cccaagtgct tgctgatgct gagcaagctg gtgttactgc cattgctaag
    12361 cctaagggag tccagtttac caaagaaggc ttgactattg atggcctaac tgccctggtc
    12421 ctcaaagtat cctcaaaaac ggctaatccc tctcagcaaa agagtcagac agataatcat
    12481 caaaccaaaa caccagatgg ctcaaaagac ctagacaaat cattaatgac taggccaaaa
    12541 agagctaaaa caaaccaaaa gctcccaaaa acgggtgaag ccccctcaaa aggcttatta
    12601 gcagccggag tagctctgct tttattggct attagcctgt tgatgaagcg ccaaaaagat
    12661 tagggtggtg ttaatagaga caaaaagatt gccgtttgcg gattcttttt gacggctata
    12721 aaagctctga gctgtgaaaa gaccttgtga ttgagaaaat tgctacttta gtgtatcatg
    12781 agaccaaaag agaaggagac atccatgaca tttgaagaaa tcgttgcgaa ttttataccg
    12841 tcgagtgttg cagaagtcac atcagctatc gctagtggca aagacatgat tgtcttttta
    12901 ggacgttcga gctgtcctta ttgtcgccgc tttgctccca agttggcaca agtagcaaca
    12961 gataatcaaa aagaagtcta ttttgtggat agcgaaaacg ctgctgatgc tgctgagctt
    13021 gctgcctttc gtgagaacta ccaattagtg acagttccag ctttgctggt tagttacgat
    13081 cagcaccaaa gggctgtatg tgattcaagt ttgacaccag atgacatctt agcttttttg
    13141 actcgtgagt aaagcactgc gcttagcgtc tcgtttaaag aagacatcag gtaacgtcta
    13201 gtcttgctat accgcttgat ttttgatata gtaagacatg acttttatag aataaaaagg
    13261 agacattatg ggatttccaa caattttaat gtttgtagtg atgcttggat tgatttggtt
    13321 tatgcaacgt caacaaaaaa aacaagcgca agaacgccaa aatcagttaa acgctattga
    13381 aaaaggagac gaagtcgtaa cgattggtgg catgtttgcg attgttgacg aagtcgatac
    13441 aacagctaaa aagattgtgt tagatgtgga tggcgtcttt ttgacatttg agctatcagc
    13501 tattaagcgt attgtaacaa aagctaccac agaaacgaca ctggttgaaa ctgaagaaac
    13561 cagtgttgtt aatgagcctg ctgttgagac agtagatagc actgacactg acagtgccat
    13621 tgaaagccat taaggcgggg aaagtttgga tgaaatatgc caaacttttt tgttttgatc
    13681 cctggactga aacagttatt ctgatgctaa caggttcatg aaaagctgac acatctatca
    13741 gaggataggt gaacattttg tgaagataag atctaattga taaaaaacaa gccaaaaggc
    13801 gtgaaatatg ataaaatatg agataagtat gcttatcaat aaggaaaaaa catgtttgga
    13861 ttaaaagcga aatcaacaaa aaaagttttg gggagtatcc caaagcatat tggaattatc
    13921 atggatggta atggacgttg ggccaaaaaa agactaaaac ctagagtgtt tgggcataaa
    13981 gctgggatgg atgctttaca agaagtaacg attactgcat ctgagttggg ggttaaagtt
    14041 ctgacagtct atgccttttc gacagaaaac tggtccagac cccaagatga ggtgtctttt
    14101 atcatgaatt taccagtaac cttctttgac aaatatgtgc ctgtgcttca tgagaacaat
    14161 gtaaagattc aaatgattgg agagactagt cgtttacctg aagatacctt ggcagcgcta
    14221 aatgcagcca tcgacaaaac aaaacgtaat acgggtttaa ttttgaattt tgctctcaat
    14281 tatggaggac gtgctgagat taccagtgct gttcgcttta ttgctcaaga tgtcttagat
    14341 gccaaattaa atccaggaga tattacggag gatttgattg ctaattactt gatgacagac
    14401 catctgccat atctctaccg agaccccgat ttgattattc gcaccagtgg tgagctgcgt
    14461 ttgagcaatt tcttgccttg gcaatcagct tacagtgaat tttatttcac accagtcttg
    14521 tggcctgatt ttaaaaaggc agagctacta aaggccattg cggattataa ccgccgccaa
    14581 cgtcgttttg gcaaggtcta aaaggagata gttatgaaag agcgtgttgt ttggggggga
    14641 gtagcagtag ctattttcct accttttctc ataataggaa acctaccatt ccaattattt
    14701 gtaggcgttt tggccatgat tggggtatca gaattactga aaatgaaaag attagaagtc
    14761 ttttcttttg aaggtgtttt tgccatgtta gccgcctttg tattggcagt tccgatggac
    14821 cattacctca cttttttacc aattgatgcg aatgttgctt tttacagctt aatggttttt
    14881 ttcattttgg caggtactgt tttaaatagc agagcttatt catttgatga tgcggccttt
    14941 ccgattgcaa ctagttttta tgttgggatt ggttttcaac atttgatcaa tgctcgcctg
    15001 tcaggaattg acaaggtctt tttggcctta tttatcgttt gggccactga tattggagca
    15061 tatctgattg ggcgtcagtt tggtagacgc aaactattgc ctagagtttc tccaaacaaa
    15121 accattgaag gtagtttagg agggatcgct tgtgcagtcc ttgtgtcttt tatctttatg
    15181 gtgattgata ggtctgttta tgcgccacat cactttttga ccatgttggt attggtcgct
    15241 cttttttcca tttttgccca atttggtgat ttggttgaaa gtgctctcaa acgtcacttt
    15301 ggtgtcaaag attcaggtaa gcttatcccg ggacatggtg gtattttaga ccgctttgat
    15361 tccatgattt ttgtttttcc catcatgcat ctctttggtt tattttaatc ttaagaggta
    15421 atgtttggag tcgcaacaac gcgacaaaac tcaaaaaagc aatagactga aaggaaatct
    15481 atgttaggaa taataacctt tattattatt tttggtattt tagtgattgt ccatgaattt
    15541 ggacatttct attttgctaa aaaatcaggc attctagtaa gagaatttgc cattggaatg
    15601 ggccctaaaa ttttttctca tgttgaccaa ggaggaactc tttatacctt gaggatgtta
    15661 cccttgggcg gttatgtgcg aatggctggt tggggtgatg acaaaactga gatcaaaaca
    15721 ggtactccag caagtttaac ccttaatgag caaggttttg ttaagcgcat caacttgtct
    15781 caaagtaagt tagacccaac gagtctccca atgcatgtta caggctatga cttagaagat
    15841 cagctgagta ttactggctt ggttttagaa gaaaccaaga catataaggt cgctcacgat
    15901 gctactattg ttgaagaaga cggtactgag ataagaattg ctccgcttga tgttcaatat
    15961 caaaatgcta gtattggcgg acgtttaatc accaattttg caggccccat gaataatttt
    16021 attttaggga ttgtggtttt tatcctcttg gtctttttac aaggtgggat gccagatttt
    16081 agtagcaatc atgtccgtgt tcaagaaaat ggagcagcag ctaaggctgg tcttcgagat
    16141 aatgaccaaa ttgtcgcaat taatggttat aaggtgacta gctggaatga tctcactgag
    16201 gctgtagacc ttgcaacacg ggatctaggc ccgtcacaga ccattaaggt cacctacaag
    16261 tcacatcagc gcttaaaaac ggtggctgtg aaaccacaaa agcatgcaaa gacatacacg
    16321 ataggagtta aggcgagtct gaaaacagga tttaaggata agctcttagg cggtctagaa
    16381 ttagcttgga gtggggcgtt tactattttg aatgctttga aaggattgat cactggcttt
    16441 agtctcaata aattaggtgg acctgttgcc atgtatgaca tgtccaatca ggctgctcaa
    16501 aacggcttag agtcagtctt atatcttatg gcaatgcttt cgatcaattt agggatcttt
    16561 aacctgattc cgattcctgc acttgatggg ggaaaaatct tgatgaatat cattgaagcc
    16621 attcgtcgca agcctatcaa gcaagaaaca gaggcctata tcaccctagc tggggttgct
    16681 atcatggtcg tattgatgat tgctgtgaca tggaatgata tcatgcgcgt ctttttctaa
    16741 tcaagatagg tcatcttaag ctgaccttat catgttaaag atcgaaaaag acctcaaccc
    16801 taagatttgc aaggattgag gtcttactca aaatgaaagg aatacacatt caaattattg
    16861 aataatcccg cttcactctt acttgatgtt tccagaaggg tttgaggata aggggatgta
    16921 ttaaaacgga ataatctatg aaacaaagta aactgcttat cccaaccttg cgcgaaatgc
    16981 caagtgatgc ccaggttatc agccacgcgc ttatggtgcg tgccggttat gtgcgccaag
    17041 tttctgctgg tatctatgct tatttaccac tggcaaatcg taccattgag aaattcaaga
    17101 ccatcatgcg tgaagagttt gaaaagatcg gtgctgttga aatgttggca ccagctcttt
    17161 tgacagctga tctctggcgt gaatcaggcc gttatgagac ctatggagag gacctctata
    17221 agcttaaaaa ccgtgataac tcagacttta tcttgggtcc gacccacgaa gaaaccttta
    17281 cgactttggt gcgtgatgcg gtcaaatctt acaagcaatt gcccttaaac ctttatcaaa
    17341 ttcagtctaa gtatcgtgat gaaaaacgtc cgcgtaatgg cttgcttcgt acccgtgagt
    17401 ttatcatgaa agacggctat agtttccatc acaactatga agatttagat gtgacctacg
    17461 aagattatcg tcaagcttac gaagctattt ttaccagagc tggtctagat ttcaaaggga
    17521 ttattggaga tggcggtgcc atggggggga aagattccca agaatttatg gccattacac
    17581 cagctcgtac ggaccttgat cgctgggtgg ttcttgacaa gtctgttgcg tcaatggatg
    17641 atattccaaa agaggtctta gaagagatta aggcagaatt ggctgcttgg atgatttcag
    17701 gtgaagatac tattgcttat tcaacagaat caagctacgc tgccaacctt gagatggcaa
    17761 ctaacgaata caaaccgtcc tcaaaagtag ctgctgaaga tgccttggca gaagttgaga
    17821 caccacattg caaaacgatt gatgaagtgg ctgcttttct ttcagtagat gaaacacaaa
    17881 ccatcaaaac cttgcttttt gtggcagata atgaacctgt tgttgctttg ctagttggaa
    17941 atgaccatat caataccgtt aaattaaaaa actatctagc tgctgatttt ttagagccag
    18001 ctagtgaaga agaagcccgt gctttctttg gtgcaggttt tggctcactt gggcctgtca
    18061 acttggcaca aggtagtcgc attgtggctg accgcaaagt gcaaaacctt accaatgccg
    18121 ttgcaggagc taacaaggat ggtttccatg tgacaggagt caatccagga cgtgacttcc
    18181 aggctgaata tgtagatatt cgtgaagtca aagaagggga aatgtctccg gatggtcatg
    18241 gtgttctcca gtttgcgcgt ggtatcgaag tcggtcatat cttcaagttg ggcactcgtt
    18301 attcagacag catgggagca acgattcttg atgaaaatgg cagagccgtt ccaattgtca
    18361 tgggatgcta tggtatcggt gttagccgta tcctgtctgc tgtcattgaa cagcatgccc
    18421 gtctctttgt gaacaagaca ccaaaaggtg attaccgtta tgcttggggg attaacttcc
    18481 ctaaagaatt ggcaccgttt gacgtgcatt tgattacggt taacgtcaaa gaccaagcag
    18541 cacaagacct aacggcgaag ttagaagctg acttgatggc taaagggtat gatgttttga
    18601 cagatgaccg taatgaacgc gtcggttcga aattctctga tagcgatttg attggtttgc
    18661 caattcgtgt cactgttggt aaaaaagccg ctgaaggtat cgtggaaatt aaaatcaagg
    18721 caacaggtga cagcattgaa gttaatgcag aaaacctcat cgaaaccctt gaaattttaa
    18781 caaaagaaca ctaaaaaaga aatgaagagg ctgcctgttt agagcggcct ctttttgctg
    18841 tgaacttgtc tgatacagag t
//
LOCUS       DAUCLR010000031        18273 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.031, whole genome shotgun sequence.
ACCESSION   DAUCLR010000031 DAUCLR010000000
VERSION     DAUCLR010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18273
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.031"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..269)
                     /locus_tag="VGT87_000900"
     CDS             complement(<1..269)
                     /locus_tag="VGT87_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEQ3813772.1"
                     /translation="MIAIEKIEKLSKENLGLITLVTGDDIGQYSQLKSRLMEQIAFDK
                     DDLAYSYFDMSEAAYQDAEMDLVSLPFFAEQKVVIFDHLLDITTN"
     gene            complement(340..2583)
                     /locus_tag="VGT87_000901"
     CDS             complement(340..2583)
                     /locus_tag="VGT87_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEQ3813773.1"
                     /translation="MTFSWTKLMPLSKIQFAFLILVLFYQIHSPSWLTFLLSLSLICL
                     LVKRLSKKEFLGVFAILSFCALFLLYQKQQLVQKLEIQPVQITSVALVPDSIRINGDQ
                     LAVLGRHGKHSYQLFYRLKSQAEAQLFKKEHRWLVMHAKVTLEKAEEVRNFKGFNYQT
                     FLAYQGIYRIGKVEQIEQLEVISPESICDYLSSLRRRAIVHCQQHFPRPMSHYLTGLL
                     FGYLDKSFGEMTDYYSQLGIIHLFALSGMQVGFFLTCFRRVLLLLAVPLEWIKWIELP
                     FACFYAALTGYSISVIRSLVQSQLRHLGIKGLDNLACTFLLVFLWDAHFLMTVGGVLT
                     FSYAFLLTVVTVEELSGAKRQLVQVLTISLGILPFLLFYFSSFNPMSMVLTALLSYLF
                     DLFILPLLCLVFCLSPLVTVSICNHLFILLEKVIQFLGNTFNSSLVFGSPTSWHLLIL
                     VISFAIFYDYRQVRQRVITCGLVIALTLLSVKYPLTNEVTFIDIGQGDSILVREWTGK
                     NLLIDVGGRPFFSSKEHWRRGHHVANAQKTLIPYLKSRGIHTIDQLLVTHADTDHMGD
                     IEVVAKAIRIKEILTSQGSLSHPSFVRRLRRLKCHVRVLAAGDQLPIMGSVLQVLYPW
                     QLGDGKNNDSLVLYGRLLNRTFLFTGDLEKEGENEIIKRYPQLRVDYLKAGHHGSNTS
                     SSAAFLDHIQPKVAFISAGKNNRYQHPHRETLARLEDRQITYYRTDTQGAIRLTGRTS
                     WHLETVR"
     gene            complement(2564..3226)
                     /locus_tag="VGT87_000902"
     CDS             complement(2564..3226)
                     /locus_tag="VGT87_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEQ3813774.1"
                     /translation="MLDELLIKGKSFLRERYFLPYLISLLLGLFLILSFSFLFWGNRQ
                     SKAAVPALREISPVKQQVSEEKKEIQEDSSILVDLKGAVQKEGVYKLTASSRVRDVIE
                     LAGGLTSEADKHAINFAEKLTDEQVVYVPKQGEEISVLPRSLVSGKKETASKDQSKVH
                     INKASLEELQHIPGIGAKRAQDIIDMRDKLGGFKALEDLRQVSGIGEKTLEKLKDDIF
                     LD"
     gene            complement(3425..4165)
                     /locus_tag="VGT87_000903"
     CDS             complement(3425..4165)
                     /locus_tag="VGT87_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEQ3813775.1"
                     /translation="MFYAYLRGLVVFLLWVVNGNAHYHHEEKMLDASENYILVAPHRT
                     FWDPVYMAFAARPKQFIFMAKKELFANRLFAWWIKMCGAFPIDRDKPSPDAIRYPVNM
                     LKKSNRSLLMFPSGSRHSQEVKGGVAVIAKMAKVKIMPAAYQGPMSVKGLLAGERVDM
                     TFGNPIDVSDIKRMNDEGIAEVANRIQAEFDRMDDELAPFQPGKARNPLTYLYRLPLG
                     LVLVVVLLLTMLFSYIASFVWNPDKHRH"
     gene            4205..5059
                     /locus_tag="VGT87_000904"
     CDS             4205..5059
                     /locus_tag="VGT87_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEQ3813776.1"
                     /translation="MKADFSKVKITLQTFTSSLFYVKITGMIKAILKEGERIDQLFSS
                     DVGIIQNKDVFSYSIDSVLLSRFPKMPSKGLIVDLCSGNGAVGLFASTRTKAAIVEVE
                     LQERLADMGQRSIQLNQLEDQVTMICDDLKNLLNHVPRSGVDLMLCNPPYFKSHESSK
                     KNVSEHYLLARHEVTTNLEEICQVARHALKSNGRLAMVHRPDRFLEIIDSLRANGLAP
                     KRVQFVYPKLGKSANMLLIEAIKDGSIEGMTILPPLVVHKENGEYTDHIFEIYFGVAS
                     KGKPNHDH"
     gene            5049..5327
                     /locus_tag="VGT87_000905"
     CDS             5049..5327
                     /locus_tag="VGT87_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEQ3813777.1"
                     /translation="MTTKKAYMYVLECVDKTLYTGYTTDLKKRLATHNAGKGAKYTRY
                     RLPVSLLYYEVFDSKEAAMSAEALFKKRKTRSQKLAYIATHQKEKKNH"
     gene            complement(5351..7351)
                     /locus_tag="VGT87_000906"
     CDS             complement(5351..7351)
                     /locus_tag="VGT87_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEQ3813778.1"
                     /translation="MSDSHLTAFDKASKAGFIIALGIVYGDIGTSPLYTMQSLVENQG
                     GVNQVSESFILGSISLIIWTLTLITTIKYVLIALKADNHHEGGIFSLFTLVRKMSPWL
                     IIPAMIGGATLLSDGALTPAVTVTSAIEGLKAVPGLSHIYQNQTNVIITTLVILIVLF
                     GIQRFGTGFIGKIFGPVMFIWFSFLGVSGFFNTLGHLEIFKAINPYYALHLLFSPENH
                     RGIFILGSIFLATTGAEALYSDLGHVGRGNIYVSWPFVKMCIVLSYCGQAAWILANKH
                     SGIELNPFFASVPSQLRVYLVSLATLAAIIASQALISGSFTLVSEAMRLKIFPLFRVT
                     YPGANLGQLYIPVINWILFAVTSCTVLAFRTSAHMEAAYGLAITITMLMTTILLKYYL
                     IKKGTRPILAHLAMAFFALVEFIFFLASAIKFMHGGYAVVILALAIVFVMFIWHAGTR
                     IVFKYVKSLNLNDYKEQIKQLRDDVCFDLYQTNVVYLSNRMQDHMIDRSILYSILDKR
                     PKRAQVYWFVNVQVTDEPYTAKYKVDMMGTDYMVRVNLYLGFRMPQTVPRYLRTIVQD
                     LMESGRLPKQEQEYTITPGRDVGDFRFVLIEERVSNARQLSNFERFIMQTKASIKHVT
                     ASPMRWFGLQYSEVTLEVVPLILSDVLKLPIKELVPVEDSEA"
     gene            complement(7478..9097)
                     /locus_tag="VGT87_000907"
     CDS             complement(7478..9097)
                     /locus_tag="VGT87_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEQ3813779.1"
                     /translation="MKFTEFNLSQDIQSAVVTAGFEKASPIQEMTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLNKIRTNENIIQALVIAPTRELAVQSQEELFRFGREKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALILDHVETLILDEADEMLNMGF
                     LEDIEAIISRVPADRQTLLFSATMPAPIKQIGVKFMKDPEHVQIKNKELTNVNVDQYY
                     VRVKEQEKFDTMTRLMDVNQPELSIVFGRTKRRVDEITRGLKLRGFRAEGIHGDLDQN
                     KRLRVIRDFKNDQIDILVATDVAARGLDISGVTHVYNYDITQDPESYVHRIGRTGRAG
                     KSGESITFVSPNEMGYLSMIENLTKKQMKPLRPATAEEAFQAKKKVALKKIERDFADE
                     TIRSNFDKFKGDAVQLAAEFTPEELALYILSLTVQDPDSLPEVEIAREKPLPFKYVGG
                     GHGNKNGKGGRGRDNRNRGDRRGSYRGDRNRDERDGDRRRQKRDKRDGHDGSGNRDFK
                     RKSKRNSKDFFNKEKKSSAKNTGFVIRHKGE"
     gene            complement(9404..10948)
                     /locus_tag="VGT87_000908"
     CDS             complement(9404..10948)
                     /locus_tag="VGT87_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEQ3813780.1"
                     /translation="MSLTEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKSGTFAKSDWMDIEKQRGISVTSSVMQFDYAGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRNIPVFTFINKLDRDGREPLELLEELEEVLG
                     IASYPMNWPIGMGRAFEGLYDLHNKRLELYKGDERFASIEDGDQLFANNPFYEQVKED
                     IELLQEAGNDFSEQAILDGDLTPVFFGSALTNFGVQTFLDTFLEFAPEPHGHKTTEGN
                     VVDPLAKDFSGFVFKIQANMDPKHRDRIAFVRIVSGEFERGMGVNLTRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     PKNVMKQKSFHKGIEQLVQEGAIQLYKNYQTGEYMLGVVGQLQFEVFKHRMEGEYNAE
                     VVMTPMGKKTVRWISEDDLDQRMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     TLEEKM"
     gene            complement(11196..11891)
                     /locus_tag="VGT87_000909"
     CDS             complement(11196..11891)
                     /locus_tag="VGT87_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEQ3813781.1"
                     /translation="MDFFAIDPIGLPHTSLIFYLSSLLIALLLVFLTFQAYRLKSYRY
                     FFLFLQLSQVIGLYTWYVLRGFPLDEALPLYHCRIAMLAIFFLPDRNKFKQLFMVLGI
                     GGTFLALLSPDLYPFRLWHVANVSFYLGHYALLVNGLIYLLRFYDASQLRLLSVVRYL
                     ATVNFLLLLVSLATKGNYGFVMDIPVIHTRHLLLNFVIVTSGLTFMVKITEYFYLKFG
                     EAQQLALAFSKEK"
     gene            complement(11995..13362)
                     /locus_tag="VGT87_000910"
     CDS             complement(11995..13362)
                     /locus_tag="VGT87_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEQ3813782.1"
                     /translation="MKLTLHEVAKIVDAQNNVSDLDDVPLHHIEFDSRKITKGDLFLP
                     LKGQRDGHEFIDLAFQNGAVATFSEKELPGKLHLLVEDCLKAFQKLAHYYIDKMRVDV
                     IAVTGSNGKTSTKDMISAVLSTTYKTYKTQGNYNNEIGLPYTVLHMPEDTEKIVLEMG
                     QDHMGDIHLLSEIARPRIAVLTLVGEAHLEYFGSRDKIAQGKMQIVDGMDSNGILIAP
                     GDSIIDPYLPENQMVIRFGDHQELNVTDIREEKDSLTFTTNVLATPVSLPLPGKYNAT
                     NAMVAAYVGKLLAVADEDIIEALESVQLTANRTEWKKAANGADILSDVYNANPTAMRL
                     ILETFANIAKNPGGKKIAVLADMKELGPDSVALHSQLIKQLSPDRIDQLVFYGDDIQE
                     LAQLAHEFYPEGHVQFFQKNQQEDQFEDMVRQVQQLLAPSDQILLKGSNSMGLAKLVN
                     HLVEN"
     gene            complement(13561..14607)
                     /locus_tag="VGT87_000911"
     CDS             complement(13561..14607)
                     /locus_tag="VGT87_000911"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEQ3813783.1"
                     /translation="MSKQTLVLLYGGRSAEREVSVLSAESVMRAVNYDKFLVKTYFIT
                     QMGQFIRTQQFSEKPSESERLMTNETIELTQKIKPSDIYEEGAVVFPVLHGPMGEDGS
                     IQGFLEVLRMPYIGTNVMSSSIAMDKITTKRVLESIGIPQVAYTVYIDGQDLEACLVE
                     TLARLTFPIFVKPANMGSSVGISKAQTKVELRKAIQLALTYDSRVLIEQGVVAREIEV
                     GLLGNDKVKSTLPGEVIKDVDFYDYQAKYVDNKITMAIPADVDQSIVTEMRSYAEVAF
                     KALGGCGLSRCDFFLTQDGQVYLNELNTMPGFTQWSMYPLLWENMGLAYPDLIEELVT
                     LAQEIFDQRESHLI"
     gene            complement(14708..15304)
                     /gene="recR"
                     /locus_tag="VGT87_000912"
     CDS             complement(14708..15304)
                     /gene="recR"
                     /locus_tag="VGT87_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEQ3813784.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSNEDVNDFA
                     KNLLAAKRELTYCSICGNLTDDDPCHICTDTSRDQTTILVVEDAKDVSAMEKIQEYRG
                     YYHVLHGLISPMNGVGPDDINLKSLITRLMDGKVSEVIVATNATADGEATSMYISRVL
                     KPAGIKVTRLARGLAVGSDIEYADEVTLLRAIENRTEL"
     gene            complement(15351..15533)
                     /locus_tag="VGT87_000913"
     CDS             complement(15351..15533)
                     /locus_tag="VGT87_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEQ3813785.1"
                     /translation="MANPNGSVTIHAKIGTAETCITDNSDQTLVTYNLNVVAYNTNNE
                     DNIAVAVHITNISAKM"
     gene            complement(16093..16872)
                     /locus_tag="VGT87_000914"
     CDS             complement(16093..16872)
                     /locus_tag="VGT87_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEQ3813786.1"
                     /translation="MKTPEQILEATIHIGEHKVTKTFLAKSILGFIGGAMISLGYLLY
                     VRIAASGLETFGAFSSIVGACAFPIGLIIILMAGGELITGNMMAVSAALLAKKIKFSE
                     LAKNWLIITLFNVIGAVFVAFVFGHFLGLTSAGIFKEEVIEVAHAKIAASPLQALVSG
                     IGCNWFVGLALWLCYGANDAAGKFLGTWFPVMTFVALGFQHSVANAFVIPAAIFEGGA
                     TWLDFVTNFIFVYSGNIIGGAIFVSFLYFKVYYHPQKSKTQ"
     gene            complement(16996..17538)
                     /locus_tag="VGT87_000915"
     CDS             complement(16996..17538)
                     /locus_tag="VGT87_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813787.1"
                     /translation="MIKKPTQLIAYTSILVAFAILIPIIMPLKLIIGPASFTLASHVP
                     LFLAIFMSIPVAILVALGTTLGFLLAGLPLIIVLRALSHLLFAILAAWWLSRKPQLMT
                     SAVKCFSFAFFINVIHGLAEFLVVYILTATTATSMSYFWSMLGLIGLGSLIHGILDFY
                     LALVLWRFLAKNLKLPTINA"
     gene            complement(17699..18220)
                     /locus_tag="VGT87_000916"
     CDS             complement(17699..18220)
                     /locus_tag="VGT87_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEQ3813788.1"
                     /translation="MKAEDRRQKIIECLNSEQKAVSATRLGKLLGVSRQVIVGDIALL
                     RAQQIDIISTPKGYIMSTALYSHQFCARIVCQHNVEETKKELEIILAHQGIITTVEVE
                     HPIYGMITAPLNIKTHSDVTNFMSKLSQSKAELLSSLTEGLHSHLISCPSQEAFLAIK
                     HDLELAGILYKGI"
BASE COUNT         5842 a         3819 c         3181 g         5431 t
ORIGIN      
        1 ttattggtcg tgatatctaa caaatggtca aaaatgacca ccttctgctc agcaaagaag
       61 ggtaggctca ctagatccat ttctgcatcc tgataagcgg cctcagacat atcaaagtaa
      121 gaataggcca aatcatcctt atcaaaagca atctgctcca ttaagcggga tttcaactgg
      181 ctatactgac caatgtcatc tcctgtgaca agggttataa gacccaaatt ttctttactc
      241 agtttttcaa tcttttctat cgcaatcatg cttttattat atcatctttc tcctaaaagg
      301 aaagcgactg cctgtttctt cgtcaggctg gaattttaac taacggaccg tttcaagatg
      361 ccagcttgtc cgaccagtca aacgaatagc tccttgcgta tccgttcggt aataagtgat
      421 ttgtctgtct tccaaacggg ctaaggtttc tctgtgagga tgctgataac ggttattttt
      481 tccagcagaa ataaaggcca ctttaggctg aatatggtct aaaaaagctg cactagaaga
      541 ggtatttgat ccatggtgac ctgcttttaa ataatctact cttagttgag gataacgttt
      601 tatgatctcg ttttccccct ctttttccaa gtcacctgtg aaaagaaaag ttcgatttaa
      661 gagcctgcca taaagtacca aagaatcgtt atttttcccg tcccctaact gccaaggata
      721 gagtacctgt aagacacttc ccataatagg caactggtct ccagctgcta aaaccctaac
      781 gtgacatttt aaacgcctta gccgcctaac aaagcttgga tggcttaaac tgccttgact
      841 agtcaaaatc tcctttatcc gaatagcttt ggcaaccact tcgatatccc ccatatgatc
      901 agtatctgcg tgagttacta atagttggtc aattgtgtga atacctctac tttttaagta
      961 aggaattaag gttttctgag cattagctac gtgatgtcct cgtcgccagt gctcttttga
     1021 agaaaagaaa gggcgcccac ctacatctat taagagattt ttccctgtcc actccctcac
     1081 caaaatacta tctccctggc caatgtcgat aaaggtcact tcattggtca aaggatattt
     1141 gacagacaaa agcgtcaacg ctataaccaa accgcaagtg ataactcgtt gtcgcacctg
     1201 ccgataatca taaaaaatag cgaagctaat caccaagata agcaagtgcc aactggtcgg
     1261 gcttccaaaa acaagggaac tattgaaagt atttccgaga aattgaatga ctttttctaa
     1321 taaaataaaa agatggttgc atattgagac agtcaccaaa ggggatagac aaaaaaccag
     1381 acaaagaagg ggcaaaataa aaaggtcaaa gagataagac aagagagctg ttaagaccat
     1441 tgacattggg ttaaaactgg aaaaataaaa gagcaaaaat ggtaatattc ctagggaaat
     1501 ggtcaagact tgtaccaact gtcttttagc tcctgataat tcttcaacag taaccactgt
     1561 taacaaaaaa gcatagctaa acgttaatac gccaccaaca gtcattaaga aatgagcatc
     1621 ccataaaaag acaagtaaaa aagtacaagc cagattgtct aatcctttta ttcctaaatg
     1681 acgcaactgc gactgcacga ggcttcgaat aacagaaata ctataacctg tcaacgcggc
     1741 ataaaagcaa gcaaaaggaa gctctatcca ttttatccat tccaaaggaa ctgcgagtaa
     1801 tagcaacacc cttctaaaac aagtaagaaa gaagccaacc tgcatccctg ataaagcaaa
     1861 gagatgaatg atacccaact gactataata atctgtcatc tcaccaaacg acttatctaa
     1921 ataaccaaaa aggaggcccg tcaggtagtg agacataggt cttggaaaat gctgctggca
     1981 atgaacaatg gctcgccgtc ttaaagagga caaatagtca catattgact cgggtgaaat
     2041 cacttcaagc tgttctattt gctctacctt ccctatcctg taaattcctt gataagccaa
     2101 aaaagtctga taattaaagc ctttaaaatt tcgaacttcc tcagcttttt ccaatgtcac
     2161 ttttgcgtgc atcactaacc aacggtgctc tttcttgaac aattgggctt ctgcttggct
     2221 tttcaaacgg taaaataact gataagaatg tttcccgtga cgtccaagaa cagctaattg
     2281 atcaccatta atccgaatac tatcaggaac taatgctaca gaggttattt gtactggctg
     2341 aatttccagt ttttgaacca actgctgttt ctgatacagc aaaaacaaag cacagaaact
     2401 aaggatggca aagacaccga gaaattcttt tttagacaaa cgcttgacaa gtaagcaaat
     2461 caaactaagg ctaaggagaa acgttaacca gctagggcta tggatttgat aaaagagaac
     2521 caagattaaa aaagcaaatt gaatctttga caggggcatt agcttagtcc aagaaaatgt
     2581 catcttttaa tttctcgagc gttttttcgc caatccccga aacctgacgg agatcttcta
     2641 acgctttaaa accacctaac ttatctctca tatcaataat atcctgagct cttttagcac
     2701 caatacctgg gatatgttgt agttcttcta agctggcctt attaatatga acctttgact
     2761 gatccttgct ggcagtttct tttttaccag aaactagtga tcttggtagc acagaaatct
     2821 cttctccttg tttaggtaca taaacaactt gctcatcagt tagtttttct gcaaaattaa
     2881 tagcatgttt atcagcttct gaagttaggc ctccagctag ttcaatgaca tctcgcacac
     2941 gactgctagc agttagctta taaactccct ctttttgtac ggcgcctttt aaatccacta
     3001 aaatactgct atcttcttga atttcctttt tttcttcact aacctgctgt ttcacaggcg
     3061 atatttctct caaagcagga acggctgcct tgctctgcct atttccccag aacaaaaaac
     3121 tgaagcttag tattagaaaa agtcctaata aaagactaat taggtatggc agaaaatacc
     3181 gctctctcaa aaacgatttt cctttaatta ataattcgtc taacattttt ttacctcatc
     3241 tttttattcg taaatgatat ccaaaagtat agcagtattg tcatttttgt gcttaaaaga
     3301 gttaagggtg tcatcgaata tcttttttag aaatgaaaaa agtgattaag cattagcggc
     3361 ttctttcaat agaagactaa tctcaatcac ttcttatttc aggcacatgt tagccctcat
     3421 gttcttaatg gcgatgttta tctggattcc aaacaaaact agcaatatag gaaaataaca
     3481 tggttaataa taataccacc accaaaacca atcctaatgg taaacggtag agataagtta
     3541 aagggttcct tgctttgcct ggttggaaag gtgccaactc gtcatccatc cgatcaaact
     3601 ctgcctgaat acggtttgcc acctcagcaa tcccttcatc attcatgcgt ttaatatctg
     3661 agacgtcaat gggattccca aaagtcatgt caacccgttc tcccgctagt aatcccttaa
     3721 ccgacatagg cccctgatag gctgctggca taatcttgac cttagccatt ttagcaatca
     3781 cagcgacccc tcctttgacc tcttgcgaat gcctacttcc acttggaaac ataagaagag
     3841 aacgattgct ctttttgagc atattaacag ggtaacgaat agcatctgga ctcggtttgt
     3901 cacgatcaat aggaaaagca ccacacattt taatccacca agcaaagaga cgatttgcaa
     3961 acaactcttt tttagccata aaaataaact gcttaggacg tgctgcaaat gccatgtaaa
     4021 ctggatccca aaaggttcga tggggtgcta ccagaatata attctcagaa gcatctaaca
     4081 tcttttcttc atgatgatag tgggcattgc cattgacaac ccataataaa aagactacta
     4141 aaccgcgtaa gtaagcgtaa aacacaggct tctcctttaa tagaataatc ttacttattg
     4201 tatcatgaaa gctgactttt cgaaagtcaa aatcactctt caaacattca cctcaagcct
     4261 attttatgtt aaaataacag gtatgataaa agcaatctta aaagaaggcg aacgtattga
     4321 tcaactcttt tcaagtgatg ttggcattat tcaaaacaag gatgtcttta gttactctat
     4381 tgatagcgtc ctcttatccc gcttccccaa gatgccttcc aaagggttaa ttgtggattt
     4441 gtgctcagga aatggggcgg ttggcttatt tgctagcact agaaccaagg cagccatcgt
     4501 tgaagtcgaa ttacaagaac ggcttgctga tatgggacaa cgctctatcc agctcaatca
     4561 actggaagac caagtaacca tgatttgtga tgatttaaaa aacctcctaa accatgtgcc
     4621 acgttcagga gttgatttga tgctctgcaa tcccccttac tttaaaagtc acgaaagttc
     4681 taagaagaac gtctctgaac attatttact ggctcgtcat gaagtgacca ctaatttaga
     4741 agagatttgt caggtagccc gtcatgccct taaatcaaat ggtcgtctgg caatggttca
     4801 tcgaccagat cgattcttgg aaattataga tagccttaga gctaatggtc ttgcccctaa
     4861 acgggtacaa tttgtctatc ctaaattagg caaatcagct aatatgttac ttattgaagc
     4921 aatcaaagac ggttctattg aagggatgac tattttacct cctttagttg tccacaagga
     4981 aaacggagaa tacactgatc atatttttga gatttatttt ggtgttgctt ccaaaggaaa
     5041 acctaatcat gaccactaaa aaagcttata tgtatgtgtt agaatgtgtt gataaaaccc
     5101 tctacactgg ttatacaact gatttgaaaa agcgtttagc tacacataac gcaggaaagg
     5161 gggctaaata tactcgctac cggttacctg tttctttact ttattacgaa gtgtttgata
     5221 gcaaagaggc tgcgatgagc gctgaagcac tctttaaaaa acgaaaaaca agaagccaga
     5281 aattggccta tattgctact catcaaaaag aaaagaaaaa tcattgagac ttatcatctc
     5341 aatgattgtt ttatgcttca ctgtcttcca caggcaccaa ttctttgatt ggtaacttca
     5401 agacatctga tagaatcaag ggaaccactt caagggttac ttcggaatat tgaagcccaa
     5461 accaacgcat tggactagcg gtaacatgtt taatgcttgc cttagtctgc atgataaaac
     5521 gttcaaagtt actcaattga cgtgcattgg agacccgttc ttctattaaa acaaagcgaa
     5581 aatcaccaac atcacgacca ggtgtgatgg tatattcctg ttcttgtttg ggtaaacggc
     5641 cactttccat caaatcctga acaatcgtgc gcaaatagcg tggcacagtc tgtggcattc
     5701 taaaaccaag ataaaggttg actcgaacca tataatccgt tcccatcata tctaccttgt
     5761 acttagctgt ataaggttca tcagttacct ggacatttac aaaccaataa acttgagcgc
     5821 gttttggccg cttgtctaaa atggagtata aaatagaccg gtcaatcata tgatcctgca
     5881 tacgattgga taggtagaca acatttgttt ggtagaggtc aaaacaaacg tcgtcacgaa
     5941 gttgtttaat ctgctccttg taatcattca aattaagaga tttcacatac ttgaaaacaa
     6001 tccgtgtgcc cgcatgccag ataaacatga caaagacaat tgcaagtgcc aagataacaa
     6061 ccgcatagcc accgtgcata aatttgatag ctgaagccaa gaagaaaatg aattctacaa
     6121 gggcaaagaa agccatcgct aaatgagcca agatgggtct cgttcctttc ttgattaagt
     6181 aatactttaa gaggattgtt gtcatcaaca tggtgatggt aatagcaaga ccataagctg
     6241 cctccatgtg ggctgatgtt ctaaaagcca aaacagtaca agaggtcact gcaaataaaa
     6301 tccagttaat gacaggaata tacaattgtc ccaaatttgc ccctgggtaa gtcacacgaa
     6361 acaaagggaa aattttcaaa cgcatggcct ctgaaaccaa ggtaaaggag cctgaaataa
     6421 gagcctgtga ggcaataata gctgccaagg ttgccaaact aacaaggtaa acccttaatt
     6481 gtgatggcac actagcaaag aaagggttta gttcaatacc tgagtgttta ttcgctaaaa
     6541 tccacgcagc ctggccacag taagacaaca caatacacat cttaacaaag ggccagctca
     6601 cataaatatt tcctcgacca acatgtccta aatccgaata aagggcttca gctcctgttg
     6661 tcgcaagaaa aatggacccc aagataaaaa tcccacgatg gttttcagga ctaaacagca
     6721 aatgcagagc atagtaagga ttgatagcct tgaaaatttc cagatgcccc aacgtattaa
     6781 aaaaaccact aacacctaga aaactaaacc agataaacat aactggtcca aaaattttac
     6841 caataaaacc tgttccaaac cgctgaatac caaacaagac tattaaaatc actagagttg
     6901 tgataatgac attggtttga ttttggtaga tgtgactcag accaggaacg gctttcaagc
     6961 cctcaatagc tgaagtaact gttaccgcag gtgtaagagc cccatcagac aaaagtgttg
     7021 cacctccaat catcgccgga ataatgagcc acggtgacat tttacgtacc aaggtgaata
     7081 aagaaaaaat cccaccttca tggtggttat ccgcttttag agcaatcaaa acgtacttga
     7141 ttgttgtgat taaggtcagt gtccaaataa ttaaagagat tgatcctaaa ataaaggatt
     7201 cagaaacctg attaacccct ccttgatttt caactagaga ctgcatcgta taaagggggc
     7261 ttgttccaat atctccataa acaataccta aagcaataat aaaacctgct tttgaagctt
     7321 tgtcaaaggc agtaagatgg ctatcagaca tcttatactc ctcctaattt tttaaaaatt
     7381 ataacaaaat aactgaaacc attataacag attccattat aaaagcaact ttttcagaaa
     7441 attaaaaaga gctgactctc atcaactctc tttaagctta ttcacctttg tgacgaatca
     7501 caaaaccagt atttttagca cttgattttt tctctttatt gaaaaagtct ttactattac
     7561 gttttgattt acgtttgaaa tcgcggttgc cactgccatc gtgaccatca cgcttatcac
     7621 gtttttgacg acgacggtca ccatcacgct catctctgtt acggtcacca cgatagctgc
     7681 cacggcgatc accacggtta cggttgtcac gtccacgacc acctttgcca tttttattgc
     7741 cgtgaccacc acccacatat ttgaatggta atggtttttc acgtgcaatt tcaacttccg
     7801 gtaagctatc tggatcttga actgttaagc tcaaaatata taaagctaat tcttcaggag
     7861 tgaattcggc agccaattgg acagcatcac ctttgaattt atcaaagttt gaacgaatag
     7921 tctcatccgc aaagtcgcgt tcaattttct tgagggctac ttttttcttg gcttgaaagg
     7981 cctcttcagc agttgctggg cgaagaggtt tcatctgttt tttcgttaag ttctcaatca
     8041 ttgagaggta gcccatctca tttggtgaga caaaagtgat agactcacca gatttaccgg
     8101 cacgacctgt acgaccaata cggtgaacat aactttctgg atcttgggta atatcgtagt
     8161 tataaacgtg ggtaactcct gaaatatcaa ggccacgcgc tgctacatca gtggcaacaa
     8221 gaatatcaat ttggtcgttt ttgaaatcac ggataacacg taagcgttta ttttggtcaa
     8281 gatcaccatg gattccttcg gcacggaaac cacgaagttt caagccacgt gtaatttcat
     8341 caactcggcg cttagtccgc ccaaaaacga tggataattc tggctggtta acatccatca
     8401 gacgggtcat agtatcaaat ttttcttgtt ctttcacacg aacatagtat tgatcaacat
     8461 tgacgtttgt taattctttg ttcttaattt gaacatgttc agggtctttc atgaatttaa
     8521 ccccgatttg tttaataggt gccggcatcg ttgcggagaa gagcaaggtt tgacggtctg
     8581 ctgggacacg actaataata gcttcaatgt cttccaagaa tcccatgtta agcatttcat
     8641 ctgcttcatc aaggattaag gtttccacat gatctaaaat aagagccttg cgcttaatca
     8701 aatctaacaa gcgacccggt gttcccacga caatgtgggc acctgattta agggctttga
     8761 tttgtttttc aatacttgat ccaccgtaaa cagagcgaac cttaacccct ttttcacgtc
     8821 caaaacggaa caattcttcc tgactttgta cagccaattc acgcgttggt gcaatgacca
     8881 atgcttggat gatattttcg ttagtacgaa tcttattcaa ggttggtaga ccaaacgctg
     8941 ctgttttacc agttcctgtt tgtgcttgac caataacatc cttcccttct agtgcgagag
     9001 gaatcgtcat ttcctggata ggagatgctt tctcaaaacc agcggtaaca acagctgatt
     9061 ggatgtcttg tgataagtta aattctgtaa atttcaattg tttctctttt ctaaaaaggc
     9121 agtgcgaagc tgccttataa accttttaat tttcgtcgtt tgattaacaa cctaactagt
     9181 gtaacacaat tagaatcaaa aagataggaa ataaggctaa tataagcaat aacacgcttt
     9241 cacgattatc cgctaaatca taaatatgct tacagttgtg aagatatcgt tttttgatag
     9301 aatcttaaat ctgcaaaaga gataactagc cactcatttc ttttaaaaag ttactcagta
     9361 aattgatctc caaaacgtta gactgttgaa aatcatgttt cggttacatt ttctcttcta
     9421 aagtgacatc tgggtatttg tctgcaaacc agcgaagggc aaaatcattt tcaaatagga
     9481 aaactggttg gtcaaaacga tccttagcta aaatgtttcg gcttgaggac atgcgttgat
     9541 ccaaatcatc ttctgaaatc cagcgaacag tctttttacc cattggtgtc atcacaactt
     9601 cagcgttgta ttcaccttcc atacgatgtt taaagacttc aaactgcagt tgacccacaa
     9661 cacccagcat gtattcgcct gtctgataat tcttataaag ctgaattgct ccttcttgta
     9721 ctaattgttc aatacctttg tggaaggatt tctgcttcat aacatttttc ggagaaacct
     9781 tcatgaagat ttctggggta aaagttggta agggttcaaa ttcaaattta tttttcccaa
     9841 ctgttaaagt atccccaact tgataagttc ctgtatcata aaccccgata atatccccag
     9901 caacagcatt cgtcacattt tcccgtgatt ctgccataaa ttgagtaaca ttggagagct
     9961 tagcgccttt gccagtgcgt gtcaaattaa ctcccatacc gcgctcaaat tcaccagaaa
    10021 caatacgcac aaaagcaata cggtcacggt gtttagggtc catgttagct tgaattttaa
    10081 agacaaaacc tgaaaaatct ttagctaagg ggtcaacaac attaccttct gtagtcttgt
    10141 gaccatgagg ttctggggca aactccaaga aagtatcaag aaaagtctga actccaaagt
    10201 tcgtaagggc agagccaaaa aagactggag ttaggtcacc atcaagaata gcttgttctg
    10261 aaaagtcatt acctgcttct tgaagaagtt caatatcttc tttgacttgt tcataaaagg
    10321 gattattggc aaacagctga tcaccgtctt caatacttgc aaaacgctcg tctcccttat
    10381 agagttctaa acgtttatta tgtaaatcat ataagccctc aaaggcgcgt cccataccaa
    10441 tcggccagtt catcggataa gaagcaatcc ctaaaacttc ttctaattct tccaagagtt
    10501 ctaaaggctc acgcccatca cggtctaact tgttaataaa ggtgaagaca ggaatattac
    10561 gatgtttgac aacttcaaaa agttttttcg tttgcgcctc aataccttta gccgaatcta
    10621 caaccatgac agccgcatcc acagccatta aggtacggta tgtatcttcc gagaaatcct
    10681 catgtccagg tgtatccaaa atattaacac gtttacccgc gtaatcaaat tgcataacag
    10741 atgaagtaac agagatacca cgttgctttt caatatccat ccagtcggac tttgcaaacg
    10801 tacctgattt tttccctttt actgtccctg cttctctaat ttcaccacca aaatataata
    10861 gttgctcagt aatagtcgtc ttaccagcat ccgggtgaga aatgatggca aaagtacggc
    10921 gttttttaat ttcttctgta agtgacattc ttttttcctt gtatagtaaa atggcagagc
    10981 ttttctctga gttattttcg ttaggtatta cttgtttatc aaaagagttg ggctacattg
    11041 ggtttctcca tatacgatta gcctctaaag ttcttcaaaa ctggtattat aacttatctt
    11101 gggctatcaa tgagtgtctt ttttaaaaaa atcgagcctg gaaatcattc cagactcttc
    11161 ttattatagc tgattttaag tgctttttca acgacctact tttctttaga gaaagcaagt
    11221 gccagctgtt gagcttcacc aaatttaagg taaaaatatt cggttatttt aaccataaaa
    11281 gtcaagccac ttgtaacgat cacaaaatta agtagcaaat gacgcgtatg aatcactgga
    11341 atatccatca caaaaccata atttcctttc gtggctaaac tgaccaacag aagaagaaag
    11401 ttaacagttg ctaaatagcg tactactgat agcagtctta actgactggc atcgtaaaaa
    11461 cgcaagaggt aaatcaaacc attcactaac aaagcataat gaccaagata aaacgagaca
    11521 tttgctacat gccataatct aaacggataa agatctggcg ataaaagtgc aaggaatgtt
    11581 ccacctatcc ccaaaaccat aaataattgc ttaaacttat tacgatcagg tagaaaaaag
    11641 atagctaaca tagcgatgcg acaatgatac aacggcaaag cttcatctaa aggaaacccc
    11701 cttaacacgt accatgtgta caagccaatc acctgagata actgtaaaaa caagaaaaaa
    11761 taacgataag attttaacct atatgcttga aaagtcaaaa aaacaagcaa aagcgctatc
    11821 aagagtgacg acaagtaaaa gataagtgag gtatgaggca gtccgatggg gtcaattgca
    11881 aaaaaatcca taaccatccc tttctacgtt ttttcattat accttaatct ttatgagaga
    11941 acaagccctt ctctgtgctt tttctaaaaa gtaaggctaa ggtaagtctt cactttaatt
    12001 ttcaactaaa tgattgacca atttagccag ccccattgag ttactccctt ttaaaagaat
    12061 ttggtcagag ggagcaagta attgttgcac ctgtcttacc atgtcttcaa actgatcttc
    12121 ttgctgattt ttttggaaga actgaacatg tccttcagga taaaactcat gtgctaattg
    12181 ggctaactct tgaatatcat caccataaaa aacaagttgg tcaatgcgat caggtgacaa
    12241 ttgtttgatt aactgcgaat gtagggcaac agaatctggt cctaattctt tcatgtcagc
    12301 tagcactgca atcttttttc caccggggtt ctttgctatg ttagcaaagg tttctaaaat
    12361 caggcgcatg gctgtcggat tggcattgta aacatctgat aagatgtcag caccattagc
    12421 agcttttttc cattctgtac gattagcagt gagttgaaca gactctaacg cctcgataat
    12481 atcctcatct gctactgcca acaatttgcc aacataggct gctaccatag cattggtcgc
    12541 gttgtatttt cctggtagag gtagggacac aggtgttgct aacacatttg ttgtaaacgt
    12601 taagctatct ttttcttctc gaatatcagt aacattcaat tcttggtggt cgccaaatcg
    12661 aatgaccatc tggttttctg gaagataagg atcgataatc gaatcacctg gggcaattaa
    12721 aatgccattt gaatccattc catcaacaat ttgcattttc ccttgagcaa tcttgtcacg
    12781 tgacccaaaa tattctaaat gggcttcgcc taccagagtt aaaacagcaa tacggggtct
    12841 tgcaatttca gagagaagat gaatatctcc catgtggtct tgtcccatct caagaacaat
    12901 tttttcggta tcttcaggca tgtggagaac ggtgtagggt aatccgatct cattattgta
    12961 atttccctgc gttttataag ttttataggt tgttgatagg actgcgctga tcatatcttt
    13021 ggtggaagtt ttaccatttg agcctgtcac agcaataaca tccacgcgca tcttatcaat
    13081 ataataatgg gctagctttt gaaaagcttt taggcagtct tcaacaagaa ggtgaagttt
    13141 ccccggcaat tctttttctg aaaaagtagc gacagctcca ttttgaaagg ctaaatcaat
    13201 aaactcatga ccatcccttt gaccttttaa gggtaaaaag aggtctcctt ttgtaatctt
    13261 tcggctatca aattcaatgt gatgcagagg aacgtcgtct aagtcagaca cattattttg
    13321 ggcgtcaact attttagcca cttcgtgtaa ggttaatttc attttgtttt ctttctaaga
    13381 acactagtat ttacgatgtc ctatctatta tagcacaaaa cattagaaaa atttctaaaa
    13441 tgtcaaactc ccttaatggc tctactattc ttcctaaaag agcaacaaaa aaaggacgaa
    13501 cgtcacatct tgacgatgtt tgccgttcca tccaagtcaa tgtttattca tctcataggt
    13561 ttaaatcaga tggctctcac gctgatcaaa tatttcttgt gccagtgtca ccagctcttc
    13621 aatcaaatct ggataagcca atcccatatt ttcccataag agtggataca tagaccattg
    13681 ggtaaagcct ggcatggtgt ttaattcgtt aaggtaaacc tgtccgtctt gggtcaagaa
    13741 aaagtcacaa cgagacaacc cacaaccacc taaggcttta aaggctactt cagcataaga
    13801 acgcatttct gtcacaatac tctggtctac atctgctgga atggccatcg taatcttatt
    13861 gtcaacatat tttgcttggt agtcgtaaaa atcaacgtcc ttgattactt ctcctggtag
    13921 agttgattta accttatcat ttccaaggag accaacttca atttctctgg caactacgcc
    13981 ttgttcaatc aaaacacgac tgtcataggt cagggctaac tgaatcgctt ttcttaactc
    14041 tacttttgtt tgggctttgg aaatccccac agaagaaccc atattagctg gttttacgaa
    14101 gataggaaaa gtaagtcttg ctaaagtttc aactaaacaa gcctccaaat cttgtccgtc
    14161 aatataaaca gtataggcaa cttgtggaat tccaattgat tctaagacac gtttagtagt
    14221 aattttatcc atagctatgc tagaagacat gacattagtc cctatatatg gcattctaag
    14281 cacttctaag aaaccttgga tagagccatc ttctcccata ggtccatgta atacaggaaa
    14341 tacaactgca ccttcttcat agatatcact tggttttatt ttttgagtta gctctatcgt
    14401 ctcatttgtc atcaagcgtt ctgattccga tggcttttct gaaaattgct gagttctgat
    14461 aaattggccc atctgagtaa taaaataagt ttttactaag aacttatcat agttgactgc
    14521 tcgcatcaca ctctctgctg aaagcactga gacttcacgt tctgctgagc ggcctccata
    14581 taatagaact aacgtttgtt tggacatagc taacctttct tatctgaatt gaatgttttt
    14641 ttattataac aaaaagatgt agccttttca aaacctctct ttgcgagtaa aggcaccaac
    14701 atgatcatta aagttcagta cgattctcaa tagctctcaa taatgttact tcatcagcat
    14761 actcaatatc tgaacctact gcgagacctc ttgccaaacg agttacctta atcccggctg
    14821 gtttcaagac acgtgaaata tacatggacg ttgcttcccc atctgctgtg gcattggtag
    14881 ctacgataac ttcgctcacc ttaccatcca ttagacgggt aattaaactt ttaaggttga
    14941 tgtcatctgg ccccacacca ttcataggcg aaatcaagcc gtgaagcaca tgataatagc
    15001 cacgatactc ttggattttt tccatggcag aaacatcttt agcatcttct actaccagaa
    15061 tggtcgtctg atcacggctc gtgtctgtgc aaatgtgaca aggatcgtca tcggtaaggt
    15121 ttccacaaat cgaacaatag gtcagttctc ttttagctgc taataaattt ttagcaaaat
    15181 cattgacatc ttcatttgac attccaatag tataaaaggc taatctcgtc gccgtcttga
    15241 taccaattcc tggaagttta gagtaactgt caattaactt tgcaatgggt gttggataaa
    15301 gcactaagtt ctcctttatt gttttccata tatcgccaca taaagcgccc tcacatcttt
    15361 gccgatatat tcgtaatatg tacagcaacc gcaatattat cttcgttatt tgtattataa
    15421 gctactacat ttaagttgta agttactaag gtctgatctg aattgtctgt gatacaagtc
    15481 tcagcagttc caattttagc gtgaatcgtg acagaaccgt ttggattagc caagtaaaaa
    15541 cttttttact cgacttgtta ccttggcctt gggatatttg gcttttgttt tttgattcat
    15601 gttacttttt tagaaaccgc aaaccatcct tatgatataa gtttatagga attgtttcat
    15661 tccatgcaga aatgatgttt actcaattga ctatgctatt tttctcaaca atagtgcata
    15721 aagcgaactc atggttatct ttaagctttc atgctacact ttcttcacta aaagtgtatt
    15781 accaaaagca ttattaagaa atctctaagc acaaaccata agacagtttg tacgcctgat
    15841 gaatctgttg ctgcatcact gtccactttt taccctatca aggctatttt tgaaagacaa
    15901 ctaatgatct acattatttt gggaactact cccatagcaa attcttttta cggatttttt
    15961 gggaatactc attttttagg gaataaacta accattattt agctaaattt tttaaacttc
    16021 gcttcttatt ttaccataaa ttataaaaag aagactgaga ataggacgcc ctcagtctaa
    16081 aaaaatattt tctcattgag ttttactctt ttgcggatga tagtaaactt taaaatataa
    16141 aaaactaaca aaaatggctc caccgataat attaccacta tagacaaaaa taaaattagt
    16201 gacaaaatcc aaccatgttg ctcctccttc aaaaatagca gcaggaatga caaaagcatt
    16261 tgctacacta tgctgaaaac caagggctac aaaggtcatg acaggaaacc aagtaccaag
    16321 gaatttacca gcagcatcat ttgccccata gcatagccac aaggccaaac caacaaacca
    16381 gttacaacca attcctgata ctagtgcctg caaagggctc gctgctattt tagcatgggc
    16441 cacctcaatc acttcttctt taaaaatacc agctgatgtc aagcctagaa aatgaccaaa
    16501 cacaaaagct acaaaaacag ctccaatcac attaaacaag gtaatgataa gccaattctt
    16561 ggcaagctcg ctaaacttaa tttttttggc caacaaagct gcagaaacag ccatcatgtt
    16621 ccctgtaatt aattctccac ccgccattaa aataataatt agaccaatcg gaaaagcaca
    16681 agctcctacg atactggaaa aagcaccaaa agtttctaaa ccactggcgg caattctcac
    16741 gtagagtaaa taaccaaggc taatcatcgc tccaccaata aagcctaaga tagatttagc
    16801 caaaaaagtt ttggtaacct tatgttcccc aatatgaata gttgcttcta aaatctgttc
    16861 tggtgttttc atgatatctc cttttgttac tgtattcaca aacacacact ttagtatatc
    16921 aattttccta aaaaatgcaa gccctttcaa caagaaaggg ctcgtcatta tcatataact
    16981 atctctattt tagtcttagg catttatggt gggaagtttt aagttttttg ctaaaaaacg
    17041 ccatagaact aaagcaagat agaaatccaa tatgccatga ataagcgaac ctaagccaat
    17101 caagcctagc atagaccaaa aataagacat agaagttgct gtggttgctg ttaaaatata
    17161 gacaactaaa aattcagcca agccatgaat gacattaatg aaaaacgcaa aactaaaaca
    17221 ttttactgcc gatgtcatca gttgtggttt tctacttaac caccacgctg ccaaaatagc
    17281 aaacaataaa tgagagagtg ctcttaaaac aataattaaa ggaagtccag ctaataaaaa
    17341 acctaatgtg gtaccaagag cgaccaaaat tgctactgga atcgacataa aaatagccaa
    17401 aaacaaagga acatggctcg ccagagtaaa ggaagctggt ccaataataa gcttaagagg
    17461 catgattatc ggaatcaaaa tcgcaaatgc gaccaaaata gaggtataag ctattagctg
    17521 tgttggtttt ttaatcattt tatctccttt tcaaaaatag tataccttaa aaaccaataa
    17581 ctatcaagac acctgtatct tttggtattt ttaaacgata ccttgttgct gataaataat
    17641 caatagaata ttttagcatg gtattcataa caaccatcaa tggcataata tactatagtt
    17701 atattccttt gtataagata cctgctaatt ctaaatcatg cttgatagct aaaaaggctt
    17761 cttgactagg acacgaaatt aagtgactat gtagtccttc tgtcaacgaa gacaataact
    17821 ctgctttaga ttgactgagt ttggacatga aattagttac atcagagtgt gtcttaatat
    17881 ttaaaggggc tgttatcata ccgtaaatag gatgttctac ctctaccgtc gtaataatcc
    17941 cttgatgagc taaaataatt tctaattctt ttttagtttc ttctacatta tgttggcaca
    18001 caatacgtgc acaaaattgg tgactataaa gcgctgttga cataatataa ccttttggag
    18061 tcgaaataat atcaatctgc tgagctcgaa gaagagctat atctccaacg attacttggc
    18121 gactcactcc taataactta cctaaacgag tagcagatac agctttttgt tcactattta
    18181 agcactcaat aattttttga cgacgatctt ctgctttcat aatctatcta tcctttttta
    18241 tttctatcat atcatatttt taagcagggt ttt
//
LOCUS       DAUCLR010000032        17227 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.032, whole genome shotgun sequence.
ACCESSION   DAUCLR010000032 DAUCLR010000000
VERSION     DAUCLR010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17227
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.032"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            40..984
                     /locus_tag="VGT87_000917"
     CDS             40..984
                     /locus_tag="VGT87_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEQ3813789.1"
                     /translation="MIDLAIAIKNHLCHNGINTIGKKTNRQGDNHLDIKQIRYFIAIV
                     ENHFNLSQAAELLYVSQPTLSMMINDFEKRENVKLFKRKRGRIIGLTYLGDNYYKDAQ
                     KVLSLYDDMFLKLHDHSKGLKGSINIGIPPLILSVVFSEVMPKLILENPGIQFNVKEI
                     GAYQLKNELLVGNVDVAVLLSPTGIADNLVETYEIQRSELSVCLSPRHRLASKKVIQW
                     EDLTDEQLALFDPSFMVHHLVLEACERHQVRPNIILTSSSWDFMLNSTKINHNVLTIC
                     PKPITELYQLKDIKCIPMERPISWRVVLTRLRKKVILK"
     gene            1158..2765
                     /locus_tag="VGT87_000918"
     CDS             1158..2765
                     /locus_tag="VGT87_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEQ3813790.1"
                     /translation="MVNIILLIVSALIGLILGYALISIRLKSAKEAAELTLLNAEQEA
                     VDIRGKAEVDAEHIKKTAKRESKANRKELLLEAKEEARKYREEIEQEFKSERQELKQL
                     ETRLAERSLTLDRKDENLSSKEKVLDSKEQSLTDKSKHIDERQLQVEKLEEEKKAELE
                     KVAAMTIAEAREVILMETENKLTHEIATRIRDAERDIKDRTVKTAKDLLAQAMQRLAG
                     EYVTEQTITSVHLPDDNMKGRIIGREGRNIRTLESLTGIDVIIDDTPEVVILSGFDPI
                     RREIARMTLESLIADGRIHPARIEELVEKNRLEMDNRIREYGEAAAYEIGAPNLHPDL
                     IKIMGRLQFRTSFGQNVLRHSVEVGKLAGILAGELGENVALARRAGFLHDMGKAIDRE
                     VEGSHVEIGMEFARKYKEHPVVVNTIASHHGDVEPDSVIAVLVAAADALSSARPGARN
                     ESMENYIKRLRDLEEIATSFDGVQNSFALQAGREIRIMVQPEKISDDQVVILSHKVRE
                     KIENNLDYPGNIKVTVIREMRAVDYAK"
     gene            2875..3510
                     /gene="gmk"
                     /locus_tag="VGT87_000919"
     CDS             2875..3510
                     /gene="gmk"
                     /locus_tag="VGT87_000919"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEQ3813791.1"
                     /translation="MSERGLLIVFSGPSGVGKGTVRQEIFSTPDHKFEYSVSMTTRPQ
                     RPGEVDGVDYFFRTREEFEELIKTGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKSKVPDGVFVFLTPPDLDELEDRLVGRGTDSQEVIAQRIERAKEEIALM
                     REYDYAVVNDEVALAAERVKRIIETEHFRVERVIGRYDKMIKITKNPFKAK"
     gene            3526..3843
                     /locus_tag="VGT87_000920"
     CDS             3526..3843
                     /locus_tag="VGT87_000920"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEQ3813792.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILQAKRAHELEAGATPTQEFKSV
                     KSTLQALEEIESGNVVIHPDPSAKREAVRAKIEAERLAKEEEERKIKEQIAKEKEEEG
                     EKI"
     gene            3908..6292
                     /locus_tag="VGT87_000921"
     CDS             3908..6292
                     /locus_tag="VGT87_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEQ3813793.1"
                     /translation="MEKVAHVIVDIPLMQTDKPFSYGIPKELVSLVQLGSRVHVPFGK
                     GNRLLQGFVIGFGQEDSSSLKLIQTVLDPEPVLNQEQLTLADQLRKTVFSYKITLLKA
                     MIPNLLNSNYDKVLRPESGLKKPDRDFLFEGKPSVLYSTLDREKEKIALKAIQAGHIT
                     VSYLAKDKKNLKTEKYYHVDLDALAVHPISSRAKKRQLLKDYLLTHTKEAKLATLYQA
                     FSRDVVAYFVTNHLIRIDERQIDRSESYFDQIKPSSFLTLNEQQASAVTEIVEQIGKP
                     SKPFLIEGITGSGKTEVYLHIIEAVLKQDKTAIVLVPEISLTPQMTSRFISRFGKQVA
                     IMHSGLSDGEKFDEWRKIKTGQAKVVVGARSAIFSPLERIGAIIIDEEHESTYKQESN
                     PRYHAREVALLRAKHHQAVVVMGSATPSIESRARASKGVYHFIQLTQRANPLAKIPEV
                     TIVDFRDYIGQQAVSNFTPYLIDKIKERLVKKEQVVLMLNRRGYSSFVMCRDCGYVDK
                     CPNCDISLTLHMDTKTMNCHYCGFQKPIPITCPECHSNSIRYYGTGTQKAFDELQGVI
                     PEARILRMDVDTTRKKRSHKTILDSFGRQEADILLGTQMIAKGLDFPNVTLVGVLNAD
                     TSLNLPDFRASEKTFQLLTQVAGRAGRAHKPGEVLIQTYNPDHYAIQLAKKQDFEAFY
                     RYEMAIRRQMAYPPYYFTVGITLSHRLEASVVKKAYQVTELLKSLLSDNIKILGPTPK
                     PIARTHNLYHYQILLKYRFEDNLEETLNRILDWSQEADNRHLKLIIDCEPQQFL"
     gene            6354..7289
                     /locus_tag="VGT87_000922"
     CDS             6354..7289
                     /locus_tag="VGT87_000922"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEQ3813794.1"
                     /translation="MIKLLFMGTPQFSATVLKGLLDNPAYEILGVVTQPDRAVGRKKD
                     IKVTPVKQLALEHGISIYQPEKLSGSQELIEIMGLGADGIITAAFGQFLPTLLLDSVS
                     FAINVHASLLPKYRGGAPIHYAIMNGDKEAGVTIMEMIKEMDAGDMVAKASTPILETD
                     NVGTLFEKLAIIGRDLLLDSLPAYLSGELKPIPQDHSQATFSPNISPEQEKLDWTMSN
                     QEVFNHIRGMNPWPVAHTFLEGQRLKIYEAQLAEGEGLPGQVIVKTKKSLVIATGQGA
                     LSLIVVQPAGKPKMSIIDFLNGIGRKLEVGDIIGR"
     gene            7279..8601
                     /gene="rsmB"
                     /locus_tag="VGT87_000923"
     CDS             7279..8601
                     /gene="rsmB"
                     /locus_tag="VGT87_000923"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEQ3813795.1"
                     /translation="MADNWKKSTRGKALLVIEAIFDQGAYTNIALNQQLSNKALSAKD
                     RALLTEIVYGTVSRKISLEWYLAHYVKDRDKLDKWVYYLLMLSLYQLIYLDKLPAHAI
                     VNDAVGIAKNRGNKKGAEKFVNAILRQFTSHPLPDMETIKRRNKYYSVKYSLPVWLVK
                     KLEDQFGSHRAVAIMESLFVRSKASIRVTDPLKLEEVAEALDAERSLLSATGLTKASG
                     HFAASDYFTNGDITIQDESSQLVAPTLNIDGDDIILDACSAPGGKTSHIASYLKTGKV
                     IALDLYDHKLELVKENANRLGVADNIETRKLDAREVHRHFEKDSFDKILVDAPCSGIG
                     LIRRKPDIKYNKESQDFNALQAIQLEILSSVCQTLRKGGIITYSTCTIFDEENRQVIE
                     AFLQSHPNFEQVKLNHTQADTVKDGYLIITPEQYQTDGFFIGQVRRVL"
     gene            8639..9379
                     /locus_tag="VGT87_000924"
     CDS             8639..9379
                     /locus_tag="VGT87_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEQ3813796.1"
                     /translation="MKISLKTDIGQKRSNNQDFINKFDNKKGITLVILADGMGGHRAG
                     NIASEMTVTDLGREWVKTDFTELSQIRDWLFETIQSENQRIYDLGQSEDFKGMGTTVE
                     AVALVESSAIYAHIGDSRIGLVHDGHYTLLTSDHSLVNELVKAGQITEEEAASHPQRN
                     IITQSIGQASPVEPDLGVRVLEPGDYLVINSDGLTNMISNDEIVTILGSKVSLDEKNQ
                     EMIDLANLRGGLDNITIALVHNESEDVE"
     gene            9376..11283
                     /gene="pknB"
                     /locus_tag="VGT87_000925"
     CDS             9376..11283
                     /gene="pknB"
                     /locus_tag="VGT87_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEQ3813797.1"
                     /translation="MIQIGKLFAGRYRILKSIGRGGMADVYLANDLILDNEDVAIKVL
                     RTNYQTDQVAVARFQREARAMAELNHPNIVAIRDIGEEDGQQFLVMEYVDGADLKRYI
                     QNHAPLSNNEVVRIMEEVLSAMTLAHQKGIVHRDLKPQNILLTKEGVVKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATIQSDIYAMGIMLFEMLTGHIPYDGDSAV
                     TIALQHFQMPLPSIIEENHNVPQALENVVIRATAKKLSDRYGSTFEMSRDLMTALSYN
                     RSRERKIIFEDVESTKPLPKVASGPTASVKLSPPTPTVLTQESRLDQTNQTNQTDALQ
                     PPTKKKKSGRFLGTLFKILFSFFIVGVALFTYLILTKPTSVKVPNVAGTSLKVAKQEL
                     YDVGLKVGKIRQIESDTVAEGNVVRTDPKAGTAKRQGSSITLYVSIGNKGFDMENYKG
                     LDYQEAMNSLIETYGVPKSKIKIERIVTNEYPENTVISQSPSAGDKFNPNGKSKITLS
                     VAVSDTITMPMVTEYSYADAVNTLTALGIDASRIKAYVPSSSSATGFVPIHSPSSKAI
                     VSGQSPYYGTSLSLSDKGEISLYLYPEETHSSSSSSSSTSSSNSSSTNDSTAPGSNTE
                     LSPSETTAQTP"
     gene            11406..12098
                     /locus_tag="VGT87_000926"
     CDS             11406..12098
                     /locus_tag="VGT87_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEQ3813798.1"
                     /translation="MKKFQFFLLIECILLAMGIMTILDNDLSSFILILVLILLALRFY
                     NQDSRNNFLLTVSLLFLFLIFMLNPYIIMAVLLGIVYIFINHFSQVKKKNRFALIRFK
                     EEKIEVNNTKHQWIGTANYESDYYCFDDINIIRISGNDTVDLTNVIVTGMDNIIVIRK
                     IFGNTTILVPIDVTVTLDVSSIYGSVDFFRCQQYDLRNESIKLKETDNQSLKKVKIIV
                     TTIAGNVEVDRR"
     gene            12095..13099
                     /locus_tag="VGT87_000927"
     CDS             12095..13099
                     /locus_tag="VGT87_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ3813799.1"
                     /translation="MKKRYYALVWLYSTITILSIVFVVMDNLGITFNYLRNHLWQVER
                     LGFSILLLIVSVTLLLLLLWIIMDDNSKRNINQNLKYILNNRRLYLDETSEINTNLSR
                     LSKKMSHLTANMQKKESAYILDSQEVVKQERKRIARDLHDTVSQELFASSLILSGISM
                     SLEQLDKTQLQTQLTTVEAMLQNAQNDLRILLLHLRPTELANRTLSEGLHMILKELTD
                     KSDIEVIYKETIAQLPKTMEDNLFRIAQEFISNTLKHAKASRIEVYLNQTSTELQLKM
                     IDDGVGFDMDQVRDLSYGLKNIEDRVNDLAGNLHLISQKGKGVSMDIRLPIVKGDDDE
                     "
     gene            13092..13733
                     /locus_tag="VGT87_000928"
     CDS             13092..13733
                     /locus_tag="VGT87_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ3813800.1"
                     /translation="MSKIKVILVDDHEMVRMGLKSFLNFQADIDVVGEASNGREGVDL
                     ALALKPDVLVMDLVMPELGGVEATLEVLKKWKEAKVLVLTSYLDNEKIYPVIDAGAKG
                     YMLKTSSAAEILNAIRKVSKGELAIETEVDKKIKAHDQHPDLHEELTAREYDILHLLA
                     KGYDNQTIADELFISLKTVKTHVSNILAKLEVDDRTQAVVYAFRHHLVPQDDN"
     gene            13770..15170
                     /locus_tag="VGT87_000929"
     CDS             13770..15170
                     /locus_tag="VGT87_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEQ3813801.1"
                     /translation="MDAKLKYKAKKIKMVFFDIDDTLRVKDTGYMPESIQRVFKALKA
                     KGILVGIASGRARYGVPQEVQDLHADYCVKLNGAYVKDDAKTIIFQAPIPADVVVAYK
                     KWADDMGIFYGMAGRHEAVLSARNDMISNAIDNVYAQLEVCPDYNEYHDVYQMWTFED
                     KGDGLQLPAELAEHLRLVRWHDNSSDVVLKGTSKALGVSKVVDHLGLKPENILVFGDE
                     LNDLELFDYAGISIAMGVSHPLLQEKADFITKKVEEDGILYALEELGLIDKELQFPQL
                     DLPNHKGPKATIKTNHGDMTLVLFPDHAPKTVANFLGLAKEGYYDGIIFHRIIPEFMI
                     QGGDPTGTGMGGQSIYGESFEDEFSDELYNLRGALSMANAGPNTNGSQFFIVQNSKIP
                     YAKKELERGGWPAPIAASYAANGGTPHLDRRHTVFGQLVDETSFQVLDLIAGVETGAQ
                     DKPKEDVIIETIEVFD"
     gene            15170..15547
                     /locus_tag="VGT87_000930"
     CDS             15170..15547
                     /locus_tag="VGT87_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEQ3813802.1"
                     /translation="MKIGDKLHGTITGIKPYGAFVALDNGTTGLIHISEIKTGFIDDI
                     DQLLAIGNQVLVQVIDIDEYSKKPSLSMRTLAEEKQHFFHRHRYSNSRHKIGFRPLEE
                     QLPQWIEESLQFLKKNTKKSLEP"
     gene            complement(15565..16506)
                     /gene="cysK"
                     /locus_tag="VGT87_000931"
     CDS             complement(15565..16506)
                     /gene="cysK"
                     /locus_tag="VGT87_000931"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEQ3813803.1"
                     /translation="MTKIYKTITELVDQTPIIKLNRLIPNEAADVYVKLEAFNPGSSV
                     KDRIALSMIEAAEAEGMISPGDVIIEPTSGNTGIGLAWVGAAKGYRVIIVMPETMSLE
                     RRQIIQAYGAELVLTPGAEGMKGAIAKAETLAIELGAWMPMQFNNPANPSIHEKTTAQ
                     EILEAFKEISLDAFVSGVGTGGTLSGVSHVLKKASPETVIYAVEAEESAVLSGQEPGP
                     HKIQGISAGFIPNTLDTKAYDQIIRVKSKDALETARLTGAKEGFLVGISSGAALYAAI
                     EVAKQLGKGKHVLTILPDNGERYLSTELYDVPVIKTK"
     gene            complement(16634..>17227)
                     /locus_tag="VGT87_000932"
     CDS             complement(16634..>17227)
                     /locus_tag="VGT87_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEQ3813804.1"
                     /translation="EESIKKSRFICHIKRVSTEEDGKNFVNAIKKEHYKANHSCFAMI
                     IGNNRQIKRSSDDGEPSGTAGIPILSVLEKQCLTNVVVVVTRYFGGIKLGTGGLIRAY
                     SNMTATAIKRFGIIEVKQQIGLEITLSYPQYQLYSNLLDQLALTETETKFSDTIKTTL
                     YCDTERVENLIDTLTNYYHGQISCEKIGSKVIEFPCD"
BASE COUNT         5520 a         3028 c         3535 g         5144 t
ORIGIN      
        1 aaataaaaat atagaatttt tataagcata taaatttttt tgatagattt ggcaatagca
       61 attaaaaatc acttatgtca taatggaatt aatactatcg gaaaaaagac taatagacaa
      121 ggggataacc atttggatat taaacaaatt cgttatttta tcgcaatcgt tgaaaatcat
      181 tttaacttga gccaggcggc ggaactgttg tatgtgtcgc agccgacact tagcatgatg
      241 atcaatgatt ttgaaaaaag ggaaaatgtt aagctcttca aacgaaaaag aggtcgtatt
      301 attgggttaa cctatcttgg tgataattat tataaggacg ctcaaaaggt tctcagtctc
      361 tatgatgaca tgtttttgaa attgcatgat cacagtaaag ggttaaaggg aagtatcaat
      421 attggtattc ctcctcttat tttatcagtt gttttctcag aagtaatgcc aaaactgatt
      481 ttagaaaatc cgggaattca attcaatgtt aaggagattg gtgcttacca actaaaaaat
      541 gaactactcg tgggaaatgt tgacgtcgca gtcttgttat ccccaacagg catcgctgat
      601 aatttggttg agacttatga aatccagaga tcagaattat ctgtatgcct gtctccacgt
      661 catcgtttag cttccaaaaa agttattcag tgggaagatt taacagatga acaacttgcc
      721 ctatttgatc ctagtttcat ggttcaccat ttagtgcttg aagcttgcga gcgtcaccaa
      781 gtcagaccta atattatttt gacgtcctct tcctgggatt ttatgcttaa ttctaccaag
      841 attaatcaca acgtcttaac tatttgtccc aaacctatta cagaattata ccaattaaag
      901 gacattaagt gtattccaat ggaacgtccc atttcttggc gtgttgtctt aacacgcctc
      961 cggaaaaaag taattctgaa atagaagcct acatcatgga cgacttgcta cagtcttttt
     1021 ttaagtaggg ttctcaacca cttggcccag atgttaattt ttttaaaaat gaattggaat
     1081 tgtgtaaacg ctcaatttat gataaactaa gaggtggctt tctattaatg aaatctagaa
     1141 tatattgagg tacaaacatg gttaatatta ttttattaat tgtttctgcc ctcattggtt
     1201 taatattagg ttatgcactt atttcgatta gactcaagtc tgcgaaggaa gctgcagagt
     1261 tgactctttt aaacgctgaa caagaagctg ttgatattcg tggcaaagca gaagtagatg
     1321 ctgaacacat caaaaaaaca gctaaacgtg aaagtaaagc aaatcgtaaa gaattacttt
     1381 tagaagcaaa agaagaggca agaaaatatc gtgaagagat tgaacaagaa tttaagtctg
     1441 aaagacaaga gcttaaacaa ctcgagacac gcttagcgga gcgctcctta actcttgacc
     1501 gtaaagatga aaacctatca agtaaagaaa aggtactaga tagtaaagaa caaagtctga
     1561 ccgataaatc taaacacatt gatgagcggc aacttcaagt ggaaaaactt gaagaggaga
     1621 aaaaagcaga actggaaaaa gttgctgcga tgacgattgc agaagcgcgt gaagtgattt
     1681 taatggagac ggaaaacaaa ctgacccatg aaattgcgac ccgcattcga gatgccgaac
     1741 gtgacatcaa ggaccgaaca gttaaaacag ccaaggactt gttagcgcaa gccatgcaac
     1801 gtcttgctgg tgagtatgtg actgaacaaa ctattaccag tgtccatctc ccagacgaca
     1861 acatgaaggg ccgaattatt ggacgtgaag gccgtaatat tcgtacttta gagagcttga
     1921 ctggcattga cgttattatt gacgatactc ctgaagttgt tatcttatca ggatttgatc
     1981 ctattcgacg tgaaattgct cgtatgacct tggaatctct gattgctgat ggtcgcatcc
     2041 atccagctcg tatcgaggaa ttggttgaga aaaatcgtct tgaaatggat aatcgtattc
     2101 gtgagtacgg tgaagctgca gcctatgaga ttggtgcacc aaaccttcat cctgatttga
     2161 ttaaaatcat gggacgcctg caattccgta cctcgtttgg tcaaaatgtc ctacgtcact
     2221 ctgttgaggt tggtaagtta gctggtattt tagctggtga gttaggtgaa aatgttgctc
     2281 ttgcccgccg tgctggtttc ttgcatgata tgggtaaagc tattgaccgt gaggttgaag
     2341 gtagtcacgt tgagattgga atggaatttg cacgtaaata caaagaacat ccagttgttg
     2401 tcaacactat tgctagccac cacggagatg tggagccaga ttctgtgatc gctgtgctag
     2461 tagctgcagc agacgctctc agttcggctc gcccaggcgc tcgtaatgag tcaatggaga
     2521 attacatcaa gcgtcttcgt gatttagaag aaatcgcgac aagttttgat ggggtacaaa
     2581 atagttttgc tctacaagct ggacgtgaga ttcgtattat ggttcaacct gaaaaaattt
     2641 cagatgatca ggttgtcatt ttgtcgcata aagtaagaga aaaaattgaa aacaatctag
     2701 attacccagg aaatattaaa gtaactgtta ttcgtgagat gagagcggtt gattatgcca
     2761 agtagaaaaa gaggaattat cctctttttc tttatgactt acctttgaaa atgttcttga
     2821 aaaagactag cctttttgat aaactattaa aaagcaacta ataaggagaa tacaatgtct
     2881 gaacgcggtt tgttaatcgt cttttctggc ccttcaggtg tcggaaaagg aacggtaaga
     2941 caagaaattt tttcaacgcc agatcataaa tttgaatatt cggtttcaat gacgactcgt
     3001 ccgcaaagac cgggcgaggt tgatggggtt gattatttct tccgcacacg tgaagaattt
     3061 gaagaactca ttaaaactgg tcaaatgcta gagtatgctg aatatgttgg taattactat
     3121 ggcacccctt taacttatgt taatgagact cttgataagg gaattgatgt ctttttagaa
     3181 attgaagttc aaggagctct tcaggttaag tcaaaagtac cagatggtgt ttttgttttt
     3241 ttaacaccac cagatttaga tgaattagaa gaccgcctgg ttggtcgtgg tacagatagt
     3301 caagaagtca tagctcaacg tattgaaaga gcaaaagaag aaattgccct tatgcgcgaa
     3361 tacgattatg cagttgttaa tgatgaagtt gccttggcag ctgagcgggt taagcgtatc
     3421 attgagacag aacactttcg tgtggagcgc gtgattggtc gttatgataa aatgattaaa
     3481 ataacaaaaa atccctttaa agctaaatag aaaagagtaa gcattatgat gttaaaacct
     3541 tccattgata ccttattaga taaagtacca tctaagtatt cacttgtgat tttacaagcc
     3601 aaacgtgccc atgaactaga agctggtgcc actccaactc aagaatttaa atcagtgaag
     3661 tcaacacttc aggcacttga agaaattgaa tcaggaaacg ttgtgattca cccagatcct
     3721 tcagctaaac gtgaagctgt ccgtgctaag attgaggcag aacgcttggc caaagaggaa
     3781 gaggaacgta aaatcaaaga acaaattgcc aaagaaaaag aagaagaagg agaaaaaatc
     3841 taaggtttca gaaatcttag attttttcat atttagtacg atgtccatga cagaaaagga
     3901 gaagcaaatg gaaaaagtag ctcatgtgat tgtagatatt ccattgatgc aaacggacaa
     3961 gccattttct tatggtattc caaaagagtt agtttctctc gtgcagcttg gttctcgtgt
     4021 gcatgttcct tttggaaaag ggaatcgttt gttgcaaggc tttgttattg gttttggcca
     4081 ggaagattca agctctctta aactgattca gaccgtttta gatcctgagc cagtcctaaa
     4141 ccaagaacaa ttgacccttg ctgatcagct tcgaaaaaca gttttttctt ataaaatcac
     4201 gctcttaaaa gcaatgattc ctaatcttct taactctaac tatgataaag tgctaaggcc
     4261 agagtcaggt ctaaaaaagc ctgatagaga ctttcttttt gaaggaaaac catctgtctt
     4321 atattctacc ttagaccgcg agaaggaaaa aatagctctt aaggccatac aagctgggca
     4381 tattactgtt agttatttag ccaaagacaa gaaaaaccta aagacagaaa aatattatca
     4441 cgtcgactta gacgccttag cggtacatcc catctctagt cgtgccaaaa agcgtcaatt
     4501 attaaaagac tatctgctaa cccatacgaa ggaagctaaa cttgcaacgc tttatcaagc
     4561 cttttctcga gatgtggtgg cttactttgt gacaaatcac ttaattcgta ttgacgaacg
     4621 tcaaattgat cgcagtgagt cctattttga ccagatcaaa ccatcttctt ttttaacctt
     4681 aaatgaacag caagcgtcag ctgttactga aattgttgag caaattggga aaccgagtaa
     4741 accttttttg attgaaggga ttacaggttc gggtaagaca gaagtttatc ttcatatcat
     4801 cgaagcagtt ttaaaacagg ataagacagc cattgtttta gtaccagaaa tttcgttgac
     4861 tcctcagatg acctctcgtt ttatttcccg tttcggaaag caagtggcta ttatgcattc
     4921 aggcttatca gatggtgaaa agtttgatga gtggcgcaag ataaaaacag gacaggctaa
     4981 ggttgtggtt ggtgcccgct ctgctatttt ctccccacta gagcggattg gtgcaattat
     5041 tattgatgag gagcatgaat caacctataa acaagaaagc aaccctagat accatgccag
     5101 agaggttgcc ctgctcagag ctaaacacca tcaagcagtt gtggttatgg gctctgctac
     5161 gccaagtatt gaaagccgag caagggctag taagggggtc tatcatttta ttcaattaac
     5221 gcaaagagca aatcctcttg ctaagattcc agaagtaacg attgttgatt ttcgtgatta
     5281 tattggacag caagcagtta gcaattttac cccatatttg attgataaga ttaaagaacg
     5341 tttggttaaa aaagaacaag ttgtcttaat gttaaataga cgtggttatt ctagttttgt
     5401 gatgtgtcgg gactgtggtt atgtggacaa atgtcccaac tgtgatatct ccttaaccct
     5461 tcacatggat actaaaacta tgaattgcca ttactgtggg tttcaaaaac ctatcccaat
     5521 tacttgtcct gagtgtcata gcaacagtat ccgctattat ggcactggaa ctcaaaaagc
     5581 ttttgatgaa ttgcaagggg ttatcccaga agctaggatc ttgagaatgg atgttgacac
     5641 cacacgaaaa aaaagaagtc acaaaaccat actagatagt tttggtcgcc aggaagcaga
     5701 tattttgtta gggacgcaaa tgattgccaa aggactggat tttccaaatg tgactttagt
     5761 tggggtacta aatgcagata cctccttaaa tttgccagat tttcgagcgt cagaaaaaac
     5821 gtttcaattg ttaacacagg ttgctggtcg ggcaggccgt gcccataagc caggagaagt
     5881 cttgattcaa acttataatc ccgatcacta tgctattcaa ttagctaaga aacaagattt
     5941 tgaagccttt taccgttatg aaatggcgat tcgccgtcag atggcttatc ccccttacta
     6001 ctttacagtg ggtattacgc tttcgcatcg tttagaagca tcggttgtaa aaaaagctta
     6061 tcaagttaca gaactgctaa agagtctctt atcggataat attaaaatat taggaccgac
     6121 tcctaagcca attgctagaa cacacaattt ataccactat caaattttgt taaaataccg
     6181 ttttgaagat aacctagaag agacgctcaa tcgtattctt gattggagtc aagaagcaga
     6241 taatcgtcat ttaaaactaa ttattgattg tgaaccgcaa caatttttat aaggtaaatc
     6301 actaaatcta ggatgatcag ttatgatata ataaaaatac aaagaggaaa gtcatgataa
     6361 aattattatt tatgggaact ccccaatttt cagcaactgt attgaagggc ttgctagata
     6421 atcctgccta tgagatttta ggagtggtta ctcagcctga tcgagctgtt ggacgcaaaa
     6481 aagatattaa ggtaacacct gtaaagcaat tagctcttga gcatggaata tcaatctatc
     6541 agccggaaaa attatcaggc tctcaagaac tcattgagat tatgggactt ggtgcagatg
     6601 gtattattac agcagcattt ggccaatttt taccaacact attacttgat tcagtttcat
     6661 ttgctattaa tgtacatgca tcattgttac ctaaatatcg aggtggagca cctatccatt
     6721 atgctattat gaatggagat aaagaggctg gcgttactat tatggaaatg attaaagaaa
     6781 tggatgcagg tgacatggtc gctaaagcaa gcactccaat tcttgaaacc gataatgtag
     6841 gtactctttt tgagaaatta gccatcattg gtcgagactt acttttggat agtttgccag
     6901 cttatctttc tggagaatta aaacctattc ctcaagatca cagccaagct actttttcac
     6961 ctaatatctc tccagaacaa gaaaaattag attggacaat gtctaatcaa gaagtgttca
     7021 atcatattcg aggtatgaac ccttggccgg tagcgcatac gtttttagag gggcaacgtt
     7081 taaaaattta cgaagctcaa ctagctgaag gagaaggatt acctggacaa gttattgtca
     7141 aaaccaaaaa atctttggta atagctactg gacaaggagc tttgtccttg atcgttgtgc
     7201 aaccggctgg taaacctaaa atgtcaataa tagacttttt aaacggcatt ggtagaaaac
     7261 ttgaagtagg ggacatcatt ggcagataat tggaaaaaaa gtacgcgagg caaagccctc
     7321 cttgtcattg aagctatttt tgatcagggg gcttacacaa acatagcgct taatcagcag
     7381 ctaagcaata aagctttatc tgctaaggac agagctctat taactgagat tgtatatggt
     7441 accgtttcac gtaaaattag cttggaatgg tatttagccc attatgtcaa agaccgagat
     7501 aaattggaca aatgggttta ttatctcctt atgctcagtc tttatcaact gatttattta
     7561 gataagttac cagcgcatgc cattgttaac gatgcggttg gtattgccaa aaatcgtggc
     7621 aataaaaaag gtgcagagaa gtttgtcaat gctattttac gtcaattcac cagccatcct
     7681 ctcccagata tggaaacaat taagcgtcga aataagtatt attcggtcaa gtattcactg
     7741 cctgtttggt tggttaaaaa gttagaagac caatttggct cccacagagc agtggctatt
     7801 atggagagtt tatttgtgcg tagtaaggct agtattcgcg tgacggaccc tttgaagtta
     7861 gaagaagttg ctgaggcttt agatgcagaa cgttcccttt tatcagcaac tggtttaacc
     7921 aaagcgtcag gtcattttgc agccagtgat tatttcacaa atggggacat cactattcaa
     7981 gatgaaagca gtcagcttgt agctcctact ttaaacattg acggtgacga cataattctt
     8041 gatgcgtgta gcgctccagg gggtaagacc tctcatatag catcttactt aaaaacaggt
     8101 aaagttattg ccttagatct ctatgatcat aaactagagt tggtgaagga aaatgctaac
     8161 cgcttaggag ttgctgataa cattgagaca agaaaactag atgccagaga ggttcatcgt
     8221 cattttgaaa aggatagctt tgataagatt ttggtggatg ctccttgttc aggaattggt
     8281 ttgattcgtc gaaaacccga tatcaaatat aataaagaaa gtcaagattt taatgctcta
     8341 caggctatcc agctagagat attgtctagt gtttgtcaaa ccttgcgtaa aggtggtata
     8401 ataacataca gtacttgcac tatttttgat gaggagaatc gtcaagtcat cgaagcattt
     8461 ttacagagtc atcctaattt tgaacaagta aaactgaacc atacacaagc agatactgtc
     8521 aaggatggtt atcttattat cacaccagaa caataccaga ctgatggctt ctttattgga
     8581 caagtcagac gagtcttgta gtattttagg aggaaactga ctgagtcaga aaaaacgtat
     8641 gaagatttca ttaaaaacag acattgggca aaaacgctca aacaatcaag attttatcaa
     8701 taagtttgat aataaaaaag gcatcacttt agtgatttta gcagacggta tgggtggcca
     8761 cagagctgga aatattgcca gtgagatgac agtcacagac ctgggaagag aatgggtaaa
     8821 aacagacttc acagagttaa gtcagattag agattggtta tttgaaacta tccagtcaga
     8881 aaatcaacgg atttatgatc taggccaatc agaagacttt aaaggaatgg gaacgactgt
     8941 tgaagcagta gctttagttg aatctagcgc catttatgct catattggtg attcacggat
     9001 tggtcttgtc catgatgggc attatacctt attgaccagt gatcattctt tagtgaatga
     9061 gttggttaag gcaggacaga tcacagaaga agaagctgct agccatccac aaagaaatat
     9121 tatcactcaa tctattggtc aagcttcccc agtagagcct gatttaggag tgagagtttt
     9181 ggagccaggg gattatcttg tcattaacag tgatggtcta accaatatga tttcaaatga
     9241 tgaaattgtg actatcttgg gcagtaaggt tagtcttgat gaaaaaaacc aagaaatgat
     9301 tgacttagct aacctaagag gaggcctaga taatattaca atcgctttgg ttcacaacga
     9361 aagtgaggac gtcgaatgat tcagattggc aaattatttg ctggtcgtta tcgcattctg
     9421 aaatctattg gccgcggtgg tatggcggat gtttatttag caaatgattt gatcttggat
     9481 aatgaagacg ttgcaatcaa ggtcttgcgt accaattatc aaacagatca ggtagcagtt
     9541 gcgcgtttcc aacgggaagc gcgggccatg gctgaattga accatcccaa tattgttgcc
     9601 atccgggata taggtgaaga agacggacag caatttttag taatggaata tgtggatggt
     9661 gctgacctaa agagatacat tcaaaatcat gctccattat ctaataatga agtggttaga
     9721 attatggaag aagtcctttc tgctatgact ttagcccacc aaaaaggaat tgtacacaga
     9781 gatttaaaac ctcaaaatat cctactaact aaggaggggg ttgtcaaagt aactgatttc
     9841 ggcatcgcag tagcctttgc agaaacaagc ttgacacaaa ctaattcgat gttaggcagt
     9901 gttcattact tgtctccaga acaggctcgc ggctccaaag cgacgattca aagtgatatt
     9961 tatgcgatgg ggattatgct ctttgagatg ttgacaggcc atatccctta tgatggcgat
    10021 agtgctgtta cgattgcctt gcaacatttt caaatgcctc ttccatctat tatcgaggag
    10081 aaccacaatg tgccacaagc tttggagaat gttgttattc gagcaacagc caagaaatta
    10141 agtgatcgtt acgggtcaac ctttgaaatg agtcgtgact taatgacggc gcttagttat
    10201 aatcgtagtc gggagcgtaa gattatcttt gaggatgttg aaagtaccaa acccctcccc
    10261 aaagtggcct caggtcccac cgcttctgta aaattgtctc cccctacccc aacagtgtta
    10321 acacaggaaa gtcgattaga tcaaactaat caaactaatc aaacagatgc tttacagccc
    10381 cccaccaaaa agaaaaaaag tggtcgtttt ttaggtactt tattcaaaat tcttttttct
    10441 ttctttattg taggtgtagc actctttact tatcttatac taactaaacc aacttctgtg
    10501 aaagttccta atgtagcagg cactagtctt aaagttgcca aacaagaact gtatgatgtt
    10561 gggctaaaag tgggtaaaat caggcaaatt gagagtgata cggttgctga gggaaatgta
    10621 gttagaacag atcctaaagc aggaacagct aagaggcaag gctcaagcat tacgctttat
    10681 gtgtcaattg gaaacaaagg ttttgacatg gaaaactaca aaggactaga ttatcaagaa
    10741 gctatgaata gtttgataga aacttatggt gttccaaaat caaaaatcaa aattgagcgc
    10801 attgtaacta atgaatatcc tgaaaataca gtcatcagtc aatcgccaag tgcgggtgat
    10861 aaatttaatc caaacggaaa gtctaaaatt acgctcagtg ttgctgttag tgatacgatc
    10921 actatgccta tggtaacaga atatagttat gcagatgcag tcaatacctt aacagcttta
    10981 ggtatagatg catctagaat aaaagcttat gtgccaagct ctagctcagc aacgggcttt
    11041 gtgccaattc attctcctag ttctaaagct attgtcagtg gtcaatctcc ttactatgga
    11101 acgtctttga gtctgtctga taaaggagag atcagtcttt acctttatcc agaagaaaca
    11161 cactcttcta gtagctcatc gagttcaacg tcaagttcaa acagttcttc aacaaatgat
    11221 agtactgcac caggtagcaa cactgaatta agcccatcag aaactactgc tcaaacacct
    11281 taacaaagaa tagttagtct catgttttac tagcatgcca ctaactattc tttttaacta
    11341 ctcgtcatat aaagatgcaa ttttccagga attctgataa aatacaaaga aataagggga
    11401 aactaatgaa aaaatttcaa ttctttttac ttatcgagtg tatactgcta gctatgggta
    11461 tcatgacaat cttggataat gatttatcaa gttttatcct aattctagtt cttatcttat
    11521 tagcattacg cttttataac caagatagcc gcaataattt tttgctaact gtcagtttat
    11581 tgtttctttt tttaattttt atgcttaatc cctacattat catggctgtt cttttgggga
    11641 tagtttacat tttcattaac catttttcac aagtcaaaaa gaagaatcgc tttgccttga
    11701 ttcgtttcaa ggaagaaaag attgaagtga ataataccaa gcatcaatgg attggtactg
    11761 ctaattatga aagtgattat tattgttttg atgacattaa catcattcga atctcaggaa
    11821 atgacacggt tgacttaacc aatgttattg tgacaggaat ggataatatc attgtgatac
    11881 gtaaaatttt tggaaatacg actatattgg tacctattga tgtcactgtt actttagatg
    11941 ttagttccat atacggaagc gttgattttt ttagatgtca gcaatatgat ttgcgcaacg
    12001 aatctattaa gttaaaagaa acggataatc aatcccttaa aaaagtcaaa attatcgtta
    12061 caactattgc cggaaatgtt gaggtggata ggcgatgaaa aaacgttact atgctcttgt
    12121 ttggctctac tcaaccatta ccattttatc tattgttttt gtggtcatgg ataatttagg
    12181 aatcactttt aactaccttc gcaatcattt atggcaggtc gaacgtctag ggttttcgat
    12241 tttattgttg attgtttctg tgactttatt attgttgtta ttgtggatta ttatggatga
    12301 taacagtaag cgtaacatca accaaaatct aaaatatatt ctcaataatc gacgactcta
    12361 tctagatgag acatcagaaa tcaatactaa tttaagtcga ctttctaaaa aaatgtctca
    12421 cctgactgct aacatgcaaa agaaagaaag tgcttatatt cttgatagcc aagaagttgt
    12481 aaaacaagag cgcaaacgaa ttgcaagaga tttacatgac acagtcagtc aagagctatt
    12541 cgcttcgtca ttgattttat caggaatttc gatgagtttg gaacaactgg acaaaacaca
    12601 attacaaaca cagttaacaa cggttgaagc aatgttgcaa aatgctcaaa atgatctacg
    12661 cattctcctt ttgcatctta gacctaccga gctagctaat cggactttat ctgagggcct
    12721 tcatatgatt cttaaggaat taacagataa aagtgatatt gaagtcattt ataaggaaac
    12781 cattgctcag cttcctaaaa caatggaaga taatcttttt agaattgccc aagaattcat
    12841 tagcaacacg ttaaaacatg ctaaagctag tcgaattgaa gtttatctca atcaaacctc
    12901 aacagaatta caattgaaga tgattgatga tggtgtagga tttgatatgg atcaggtaag
    12961 ggatttgagt tatggtctga agaatattga agaccgtgtc aatgatttag caggaaacct
    13021 acatttaatt agtcaaaaag gcaaaggagt ttccatggat attagactgc cgatagtgaa
    13081 gggagatgac gatgagtaag ataaaagtga tattggtcga tgatcatgaa atggtccgca
    13141 tgggactcaa gagttttttg aattttcaag ctgatattga tgtcgttggt gaggcctcta
    13201 atggacgtga aggggttgat ttagcattgg ctttgaagcc agatgttttg gttatggatc
    13261 tagtgatgcc agagttaggc ggtgttgagg caactttaga agtcctaaaa aaatggaaag
    13321 aggctaaggt acttgtgtta acttcctatc tagataatga aaagatatac cctgtcattg
    13381 atgcaggagc gaagggttat atgttaaaaa catcgagtgc ggctgaaatt ttaaatgcca
    13441 ttcgcaaggt ttcaaaggga gagttagcca ttgaaacaga agttgacaaa aaaattaagg
    13501 cgcatgatca acaccctgac ttgcatgagg aactaacagc gcgtgagtat gatattttac
    13561 accttttagc taaagggtat gataatcaga ccatcgctga tgaactcttt atttccttaa
    13621 aaaccgtcaa aacacatgtg tccaatattc tagccaagtt agaagttgat gaccgaaccc
    13681 aagcggttgt ctatgctttt cgacaccatt tagtccccca agacgataat taatgctata
    13741 atatagagta tagcaaattg gaggaaagta tggacgcaaa actaaaatac aaagctaaaa
    13801 aaatcaaaat ggtgttcttt gatattgatg ataccttgag ggtcaaagat acgggttata
    13861 tgcctgagtc gattcaaaga gtatttaagg ctttgaaggc aaaaggcatc ttggttggta
    13921 ttgcttcagg tcgcgctcgg tacggtgttc cacaagaagt tcaagattta catgctgact
    13981 attgtgtcaa attaaatgga gcttatgtca aagacgatgc taaaactatt attttccaag
    14041 ctccgattcc agcagatgtt gttgtggcct ataaaaaatg ggcggatgat atgggcattt
    14101 tttatggaat ggcgggtcgt catgaagcgg tgttgtccgc tcgaaatgac atgattagca
    14161 atgctattga taatgtctac gctcaactag aggtctgtcc ggattataat gagtatcatg
    14221 atgtttacca gatgtggact ttcgaggaca agggagatgg cttgcagttg cctgctgaat
    14281 tagcagagca ccttcgtttg gtcagatggc acgataactc atcagatgtt gtcttgaaag
    14341 gtacgtcaaa agcgctaggt gtttcaaaag tagttgacca tttaggatta aaacctgaaa
    14401 acattttggt ttttggagat gaactcaatg acctagagtt atttgactat gctggtatca
    14461 gtattgcaat gggagtatca catccattgc tgcaagaaaa agcagatttt atcacaaaaa
    14521 aagttgaaga agatggcatt ttatatgcct tggaggaatt aggattgatt gacaaagaat
    14581 tacagtttcc acaattggat ttgccaaacc ataaaggacc aaaagcaacc attaaaacaa
    14641 accatggcga tatgaccctg gtcttgttcc cagatcatgc tcctaaaaca gtagccaatt
    14701 tcttaggttt ggctaaagaa gggtactatg atgggattat cttccaccgt attattcctg
    14761 aatttatgat tcaagggggc gatccaacag gaacaggaat gggtggacaa tccatctatg
    14821 gtgaaagttt tgaagatgaa ttttcggatg aactctataa tctccgtggg gccttgtcga
    14881 tggctaatgc gggaccaaat actaacggca gtcagttctt tattgttcaa aatagtaaga
    14941 ttccttatgc caaaaaagaa ctagaacgtg gtggttggcc ggctccaatt gcagcttctt
    15001 acgctgcaaa tggtggaact cctcaccttg atcgtcgtca tacggtcttt ggtcagcttg
    15061 tggacgaaac ttcattccaa gttttggatt tgattgctgg cgttgaaact ggcgcacagg
    15121 ataaacctaa agaagacgtt atcattgaaa caatcgaggt atttgactaa tgaaaattgg
    15181 cgacaaactg catggcacca tcactgggat taaaccatac ggagcctttg ttgcccttga
    15241 caatgggaca acaggtctca ttcatatttc agaaataaaa acgggattta ttgacgatat
    15301 tgatcaatta ttggctatag gcaaccaggt tttagtacaa gtaattgata ttgatgaata
    15361 tagtaaaaag ccaagtttat ctatgcggac cttagcagaa gaaaaacagc attttttcca
    15421 tcgtcatcgt tattccaaca gtcggcacaa gattggcttt aggccacttg aagagcagtt
    15481 acctcagtgg attgaagaaa gcctgcagtt tttaaaaaag aacactaaaa agagtctgga
    15541 accttagtag ttccaaaccc ttttttattt cgtcttaatt actggtacat catagagttc
    15601 agtcgataaa tagcgttcgc cattatctgg taaaatagtt aacacatgtt tgccttttcc
    15661 taactgttta gcgacttcaa tagcggcgta aagagcagct ccagaagaaa tcccaaccag
    15721 gaagccttcc ttagctcctg ttagtcgagc agtttctaaa gcatctttcg atttaacacg
    15781 gataatttgg tcataggctt tggtatctaa cgtgtttggg ataaatccag ctgatatacc
    15841 ttgaatttta tgtggtccag gctcttgacc agataagaca gcagattctt cagcttcaac
    15901 agcatagata acagtttcag ggctagcttt tttcaagaca tgtgaaacac cagaaagtgt
    15961 tcctccagta ccaacaccag atacgaatgc atctaaagaa atctccttaa aagcttccaa
    16021 aatttcttga gctgttgttt tttcatggat gcttggattg gcagggttat taaattgcat
    16081 aggcatccaa gcacctagtt ctattgctaa agtttcagct tttgcaatag cccctttcat
    16141 accttctgct ccaggtgtta agacaagctc tgcaccataa gcctgaatga tttgccgtct
    16201 ttccaagctc atagtttcgg gcataacaat aatgactcga taccctttag cagcacctac
    16261 ccatgcaaga ccaatacctg tattaccact tgttggttcg ataataacgt caccaggact
    16321 tatcatacct tcagcttcag cagcttcaat catcgataaa gcaatacgat ctttaacaga
    16381 agatcctggg ttaaaagctt ctaattttac ataaacgtca gcagcttcgt ttggaattaa
    16441 acggttaagt ttgataatag gtgtttgatc tactaattct gttatagttt tgtaaatttt
    16501 agtcatagtt tagcctcttt tcttgataca aaataaatac gctgtgtatt taagtataag
    16561 gagagatatt attaaagtaa aatacagatt tactatcact ctgataataa agccatatca
    16621 cttttgttga tgattaatca caggggaatt caataacttt ggatccgatt ttctcacagc
    16681 ttatctgacc atgataataa tttgttaaag tatcaatgag attttccaca cgttcagtat
    16741 cacaataaag tgttgtcttt atcgtgtctg aaaacttagt ttctgtttcg gttaaagcca
    16801 attgatctag caagttagag tatagttgat attgaggata agaaagtgtg atttctagtc
    16861 ctatctgctg tttaacttca atgatgccaa atcgtttaat agctgttgcg gtcatgttag
    16921 agtatgctct gattaaccca cctgttccta atttaatacc accaaagtaa cgcgttacca
    16981 caactactac atttgttaga cactgctttt ctaacacaga aaggataggt attccagcag
    17041 ttcctgaagg ttcaccatca tcacttgagc gcttgatctg tctgttgtta ccgataatca
    17101 tcgcaaagca ggaatgatta gctttgtaat gttctttttt gatagcatta acaaaattct
    17161 taccatcttc ttcagtgctc actcgtttga tatgacaaat gaaacgagat tttttaatac
    17221 tttcttc
//
LOCUS       DAUCLR010000033        16467 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.033, whole genome shotgun sequence.
ACCESSION   DAUCLR010000033 DAUCLR010000000
VERSION     DAUCLR010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16467
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.033"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            291..3023
                     /locus_tag="VGT87_000933"
     CDS             291..3023
                     /locus_tag="VGT87_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpnA family nuclease"
                     /protein_id="HEQ3813805.1"
                     /translation="MINKKYIIPVSLLTLAITLTSVEEVTSRQNLTYANEIVTQRPKR
                     ESVISDKSNFPVISPYLASVDFGERKTPLPTPDKGVKVTTEQSIAQVRKGPEERTYTV
                     TGKITSVINGWGGYGFYIQDSEGIGLYVYPQKDLGYSKGDIVQLTGTLTRFKGDLQLQ
                     QVTAHKKLELSFPTSVKEAVISELETTTPSTLVKLSHVTVGELSTDQYNNTSFLVRDD
                     SGKSIVVHIDHRTGVKGADVVTKISQGDLINLTAILSIVDGQLQLRPFSLEQLEVVKK
                     VTSSNSDASSRNIVKIGEIQGASHTSPLLKKAVTVEQVVVTYLDDSTHFYVQDLNGDG
                     DLATSDGIRVFAKNAKVQVGDVLTISGEVEEFFGRGYEERKQTDLTITQIVAKAVTKT
                     GTAQVPSPLVLGKDRIAPANIIDNDGLRVFDPEEDAIDYWESMEGMLVAVDDAKILGP
                     MKNKEIYVLPGSSTRPLNNSGGVLLPANSYNTDVIPVLFKKGKQIIKAGDSYKGRLAG
                     PVSYSYGNYKVFVDDSKNMPSLMDGHLKPEKTNLQKDLSKLSIASYNIENFSANPSST
                     KDEKVKRIAESFIHDLNAPDIIGLIEVQDNNGPTDDGTTDATQSAQRLIDAIKKLGGP
                     TYRYVDIAPENNVDGGQPGGNIRTGFLYQPERVSLSDKPKGGARDALTWVNGELNLSV
                     GRIDPTNVAWKDVRKSLAAEFIFQGRKVVVVANHLNSKRGDNALYGRVQPVTFKSEQR
                     RHVLANMLAQFAKEGAKHQANIVMLGDFNDFEFTKTIQLIEEGDMVNLVSRHDISDRY
                     SYFHQGNNQTLDNILVSRHLLDHYEFDMVHVNSPFMEAHGRASDHDPLLLQLSFSKEN
                     DKAESSKQSVKAKKTSKGKLLPKTGDSLVYVITLLGTASLLVPILLLTKGKKES"
     regulatory      3188..3303
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            3301..3801
                     /locus_tag="VGT87_000934"
     CDS             3301..3801
                     /locus_tag="VGT87_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEQ3813806.1"
                     /translation="MTKLTVHDYVHIGLVAALYVVLTITPPLNAISYGMYQFRISEMM
                     NFLAFYHRKYIIAVTLGCMIANFYSFGLIDVFVGGGSTLIFVTLGVILFSKYQKDYLF
                     NGIFNKAFVYFSFFFATSMFTVAIELYFFGAPFLLTWFTTALGELVSLLIGSLIIDKL
                     SQRISF"
     gene            3995..5953
                     /gene="ligA"
                     /locus_tag="VGT87_000935"
     CDS             3995..5953
                     /gene="ligA"
                     /locus_tag="VGT87_000935"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEQ3813807.1"
                     /translation="MKKRIKELTDLLNRYRYDYYTKDAPSVSDSDYDKLYRELVTLEQ
                     SYPEYVLQDSPTQQVGGTILKGFEKYRHQYPLFSLQDAFSREELDAFDKRVKAEFPNA
                     TYLAELKIDGLSISLSYENGFLQVGATRGDGNIGENITENIKKIKDIPHQLSEPLTIT
                     VRGEAYMSRQSFKAINEARQENGETEFANPRNAAAGTLRQLDTAVVAKRELATFLYQE
                     ASPTARNQQNEVLAELADLGFSVNPYYQLTSSMDEIWDFIKTIEAKRDQLAYDIDGVV
                     IKVNSLAMQEELGFTVKAPRWAIAYKFPAEEKEAEILSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRIGDTVIVYKAGDIIPAVLNVVMSKRNQQEVMLIP
                     KLCPSCGSELVHFEDEVALRCINPLCPSLIQRSLEHFASRDAMNITGLGPAIVEKLFL
                     AGFVHDVADIYQLTKENFMQLDGIKEKSADKLLAAIEASKSNSAEKLLFGLGIRHIGS
                     KVSRLILEVYGDISALLTAKEEEIARIDGLGSTIAQSLTQYFEQKTAAILVDELKTAG
                     VNMHYSGQKVNSDAALFGLTVVLTGKLNQLNRNEAKDKLEALGAKVTGSVSKKTDLVI
                     AGSDAGSKLEKAKSLGIRIEDEDWLRKL"
     gene            5967..6989
                     /locus_tag="VGT87_000936"
     CDS             5967..6989
                     /locus_tag="VGT87_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEQ3813808.1"
                     /translation="MKKQLRARLIYNPTSGQELMRKSVPEVLDILEGFGYETSAFQTT
                     AQKNSALNEARRAAKAGFDLLIAAGGDGTINEVVNGIAPLKKRPKMAIIPTGTTNDFA
                     RALKVPRGNPSQAAKLIGKNQTIQMDIGRAKKDTYFINIAAAGSLTELTYSVPSQLKT
                     MFGYLAYLAKGVELLPRVSNVPVKITHDKGVFEGQVSMIFAAITNSVGGFEMIAPDAK
                     LDDGMFTLILIKTANLFEIVHLLRLILDGGKHITDRRVEYIKTSKIVIEPQCGKRMMI
                     NLDGEYGGDAPITLENLKNHITFFADTDLISDEALVLDQDELEIEEIVKKFAHEVEDL
                     EQELEE"
     gene            7381..7578
                     /locus_tag="VGT87_000937"
     CDS             7381..7578
                     /locus_tag="VGT87_000937"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEQ3813809.1"
                     /translation="MNPIFALALACFGVSLAEGFLMANLFKAASRQPEIIGQLRSLMI
                     LGVAFIEGTFFVTLVMAFILK"
     gene            7613..8329
                     /gene="atpB"
                     /locus_tag="VGT87_000938"
     CDS             7613..8329
                     /gene="atpB"
                     /locus_tag="VGT87_000938"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEQ3813810.1"
                     /translation="MEEAKIPMLKLGPITFNLTLLAVCIVTIAVIFAFVFWASRQMKL
                     KPEGKQTALEYLISFVDGIGEEHLDHNLQKSYSLLLFTIFLFVAVANNLGLFTKLETV
                     NGYNLWTSPTANLAFDLALSLFITLMVHIEGVRRRGLVAHLKRLATPWPMTPMNLLEE
                     FTNFLSLAIRLFGNIFAGEVVTGLIVQLANYRVYWWPIAFLVNMAWTAFSVFISCIQA
                     FVFTKLTATYLGKKVNESEE"
     gene            8347..8841
                     /gene="atpF"
                     /locus_tag="VGT87_000939"
     CDS             8347..8841
                     /gene="atpF"
                     /locus_tag="VGT87_000939"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEQ3813811.1"
                     /translation="MSITFGELVGNFILVTGSVIVLLLLIKKFAWGAIESILQTRSQQ
                     ISRDIDQAEQSRLSAQQLEAKSQANLDASRSQASKIISDAKEIGQLQGDKLVAEATDE
                     AKRLKEKALTDIEQSKSDAISAVKTEMSDLTVLLAEKIMGANLDKTAQSQLIDSYLDD
                     LGEA"
     gene            8841..9377
                     /locus_tag="VGT87_000940"
     CDS             8841..9377
                     /locus_tag="VGT87_000940"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEQ3813812.1"
                     /translation="MTKKEQALIEQYAKSLVEVASEHHSLDALQADVLAILETFVTTN
                     LDQSLSSLAVPHAEKIKLLTLLKGNNSVYMNNFLNLILQNEREAYLYQMLQAVLNEIA
                     IVSNQYDVTVTSSLPLTEEQKSRVRAVVAKKFAVTAGRLIEKVDPSLIGGFIISVNNK
                     VIDTSIRRQLQAFKMNLK"
     gene            9393..10901
                     /locus_tag="VGT87_000941"
     CDS             9393..10901
                     /locus_tag="VGT87_000941"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEQ3813813.1"
                     /translation="MAINAQEISALIKKQIENFQPNFDVTETGIVTYIGDGIARARGL
                     DNAMSGELLEFENGAYGMAQNLESNDVGIIILGDFSAIREGDVVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPVDGLGDIETTGFRPVETPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTSVAIDAILNQKGQDMICIYVAIGQKESTVRTQVETLRRYGAL
                     DYTIVVTASASQPSPLLFIAPYAGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERSAKVSDDLGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLQENLFNSGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQPLHKPLPVEKQVVILYALTHGFLDDVPV
                     DDILAFEEALYDYFDVHYNDLFETIRTTKDLPEEAALDAAIKAFKEHSNFKS"
     gene            10917..11792
                     /locus_tag="VGT87_000942"
     CDS             10917..11792
                     /locus_tag="VGT87_000942"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEQ3813814.1"
                     /translation="MAGSLSEIKAKIISTEKTSKITSAMRMVSSAKLVKSEQAARDFQ
                     IYASKIRQITTDLLKSELTIGSDNPMLVSRPVKKTGYIVITSDKGLVGGYNSKILKSV
                     MDMITEYHADGDYEIISIGSVGSDFFKARGMNVAFELRGLADQPSFEQVRQIISQSVD
                     MFVNEIFDELYVCYNHHVNSLTSQVRVQQMLPISDLVADEAAEEGVTGFELEPNRHDI
                     LDQLLPQFTESLIYGAIIDAKTAEHAAGMTAMQTATDNAKNVINDLTIQYNRARQAAI
                     TQEITEIVAGANALE"
     gene            11954..13360
                     /gene="atpD"
                     /locus_tag="VGT87_000943"
     CDS             11954..13360
                     /gene="atpD"
                     /locus_tag="VGT87_000943"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEQ3813815.1"
                     /translation="MSSGKIAQVVGPVVDVMFASGDKLPEINNALIVYKDSDKKQKIV
                     LEVALELGDGMVRTIAMESTDGLTRGLEVLDTGRAISVPVGKETLGRVFNVLGETIDL
                     EEPFAEDVDRQPIHKKAPSFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTQKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLTQ
                     MGIYPAVDPLASSSRALSPEIVGEEHYAVATEVQRVLQRYRELQDIIAILGMDELSDE
                     EKTLVGRARRIQFFLSQNFNVAEQFTGLPGSYVPVAETVRGFKEILEGKYDHLPEDAF
                     RSVGPIEDVIKKAEKMGF"
     gene            13373..13789
                     /locus_tag="VGT87_000944"
     CDS             13373..13789
                     /locus_tag="VGT87_000944"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEQ3813816.1"
                     /translation="MTQMTVQVVTPDGIKYDHHAKCISVTTPDGEMGILPNHINLIAP
                     LQVHEMKIRRGGEDEKVDWIAINGGIIEIKDNVVTVVADSAERDRDIDVSRAERAKLR
                     AEREIAQAETTHNIDEVRRAKVALRRALNRINVSKK"
     gene            14055..14312
                     /locus_tag="VGT87_000945"
     CDS             14055..14312
                     /locus_tag="VGT87_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEQ3813817.1"
                     /translation="MVCYNNPMELLMKIIAFSCHLFFIGLSYQLLISVIDWTKFSHYH
                     PENLGRLRLFVFLLATALGYLVSHCMLELLQISQTLFFEFR"
     gene            14377..15648
                     /gene="murA"
                     /locus_tag="VGT87_000946"
     CDS             14377..15648
                     /gene="murA"
                     /locus_tag="VGT87_000946"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEQ3813818.1"
                     /translation="MDKIIIEGGQTRLEGEVVIEGAKNAVLPLLAASILPSKGKTILR
                     NVPILSDVFTMNNVVRGLDIRVDFNEAANEITVDASGHILDEAPYEYVSQMRASIVVL
                     GPILARNGHAKVSMPGGCTIGSRPINLHLKGLEAMGATITQKGGDITAQADRLQGAMI
                     YMDFPSVGATQNLMMAATLADGVTTIENAAREPEIVDLAQFLNKMGARIRGAGTETLT
                     ITGVTHLRGVEHDVVQDRIEAGTFMVAAAMTSGNVLIRDAVWEHNRPLISKLMEMGVS
                     VTEEEYGIRVQANTPKLKPVTVKTLPHPGFPTDMQAQFTALMAVVNGESTMVETVFEN
                     RFQHLEEMRRMGLQSEILRETAMIHGGRQLQGAPVMSTDLRASAALILTGIVAQGVTI
                     VNNLVHLDRGYYQFHEKLAKLGATISRSSEV"
     gene            15652..15840
                     /locus_tag="VGT87_000947"
     CDS             15652..15840
                     /locus_tag="VGT87_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEQ3813819.1"
                     /translation="MTNGLKYVLEQMLLLFIIAALACLFLAIGLMIGYSFMGDGHSPW
                     HILSMDKWAELVNKFTGK"
     gene            15876..16467
                     /locus_tag="VGT87_000948"
                     /pseudo
     CDS             15876..16467
                     /locus_tag="VGT87_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990412.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
BASE COUNT         5081 a         2837 c         3514 g         5035 t
ORIGIN      
        1 ggtgtaaaaa cgtatttatt ttatgaaatg tttttgttac aataagcatg tatcgaatgc
       61 tataaagctg tcttgttcgc tatcaggggt gcttgtgatg ttatgtcaga tcaaacaagt
      121 ctttaattgg gtgacttaac agattaaaag aaaagatcta atcgtgttac ttgatagtat
      181 aattaatgca aacagaaagt gtatcccaat tcatagtctc aggtggaggt taatattcaa
      241 aaataaaatc ttgttgttgt cttaaactaa tcacattaaa aggagatatc atgattaaca
      301 agaaatatat aatacctgtt tcattgttga cactagctat tacgcttact agtgttgaag
      361 aagttacttc acgccaaaat ttgacttatg ccaatgaaat cgtaacacaa aggccaaaga
      421 gagaatctgt tattagtgat aaatcgaatt ttcccgtcat atcaccttac ctagcaagtg
      481 tggattttgg tgagagaaaa acacctttgc caacacctga taaaggagta aaagtaacta
      541 ctgaacagtc tattgctcaa gtaagaaagg ggcctgaaga aagaacctat actgttactg
      601 gcaagattac gagtgtgatc aatggctggg gaggctatgg cttttatatt caagatagtg
      661 aaggtattgg actttatgtt tatcctcaaa aagatttagg atacagtaag ggagatattg
      721 ttcaattaac aggtacactt actcgcttta aaggtgattt acaactccaa caggtgactg
      781 cacacaaaaa gttagagtta tcttttccga cttctgttaa agaagcagta atatcagaat
      841 tagaaacaac aacaccctca acattagtta agttatctca cgtgacagtt ggagaattat
      901 caactgatca atataacaac acatctttcc ttgtaaggga tgatagtggt aaaagtatag
      961 ttgttcatat agatcatcgt acaggggtta aaggggctga tgttgttact aaaataagtc
     1021 agggtgattt gattaacctc acagccatat tgtctattgt tgatggtcag ttacaattaa
     1081 gaccgttttc tcttgaacaa ttggaagtgg ttaaaaaggt cacaagctca aatagtgatg
     1141 cttcatctcg taatattgtg aaaataggcg agattcaagg agctagtcat acgtcgccac
     1201 ttctcaaaaa agcggtcacc gtagaacagg ttgttgtcac ttatttagac gattccactc
     1261 atttttatgt tcaagatctt aatggtgatg gtgatttagc gacttcagat ggtattcgtg
     1321 tttttgctaa aaacgctaag gttcaagtcg gcgatgtttt gaccatttca ggtgaagtgg
     1381 aagaattctt tggtcgtggt tatgaggaac gtaagcagac tgaccttacc atcacccaaa
     1441 ttgtggctaa agcagtgacc aaaacaggga cagctcaagt tccatcaccg cttgttttag
     1501 ggaaagaccg tatcgcgcca gccaatatta ttgataatga tggcttgcgt gtgtttgatc
     1561 cagaagaaga cgctattgat tattgggaat caatggaagg catgttagtg gcggttgatg
     1621 atgctaaaat ccttggtcca atgaaaaata aagaaattta tgtcttacct ggctctagta
     1681 caagaccgtt aaataattca ggtggagtat tacttccagc taattcttat aacacagatg
     1741 tgattcctgt tcttttcaaa aaaggcaaac aaattattaa agcaggagac tcttacaaag
     1801 gaagattagc tgggccagta tcttatagct atggtaatta caaggtcttt gttgatgaca
     1861 gcaaaaacat gccaagttta atggatggtc atctaaaacc tgaaaaaaca aacttgcaaa
     1921 aagatcttag taagttaagc attgcttctt acaatattga aaacttctca gccaatcctt
     1981 cttcaactaa agatgagaag gtcaaacgga ttgccgaatc ctttattcat gatctgaatg
     2041 ctccagacat cattggatta attgaagtcc aagataataa tgggccgact gatgatggga
     2101 caacggatgc gacacaaagc gcgcaacgcc tcattgatgc tattaaaaaa ctaggcggcc
     2161 caacttatcg ttatgttgat attgctccag aaaataatgt tgacggaggt caaccaggtg
     2221 gtaatattcg aacaggattc ctctatcaac cagagcgcgt cagcctttct gataagccaa
     2281 aaggcggtgc tcgtgatgct ctaacttggg ttaatggaga attaaacctt agtgttggtc
     2341 gaattgatcc aactaacgtc gcttggaaag atgttcgtaa atcactagca gcagaattta
     2401 tcttccaagg tcgtaaagtc gttgttgttg caaatcattt gaactctaag cgtggggata
     2461 atgctcttta tggtcgtgtg caaccagtca cttttaaatc tgagcaaaga cgtcacgtct
     2521 tggctaatat gctagcacaa tttgcgaaag aaggggcaaa acaccaagct aatattgtga
     2581 tgctaggtga ctttaatgat tttgaattca caaagacgat tcaattaatc gaagaaggtg
     2641 atatggttaa cttggtgagc cgacatgata tttcagatcg gtattcttat tttcaccaag
     2701 gcaataatca gacccttgat aatatattag tttcacgcca tttacttgat cactacgaat
     2761 ttgacatggt tcatgtgaat tccccattta tggaagctca cggacgcgca tcagatcatg
     2821 atccattgtt acttcaatta tcattttcta aagaaaatga taaggcagag tcttctaaac
     2881 aaagtgtaaa agctaaaaaa acttcaaaag gaaaactgtt gccaaaaaca ggagatagtc
     2941 ttgtttatgt gataacacta ctaggaacgg ctagtttatt agtgcctatt ttattattga
     3001 ctaaaggcaa aaaggaatca tagcgatttg aagaattgga gttcataaag agtcgttaaa
     3061 ggcttgatat acttacgtta gaccttaagc taaaatggat gactatataa aaactaagat
     3121 tgataacaaa agaagataac aataagacag ctttacttgc taaagacttt tgtcaagcta
     3181 attgttttat ggtacaatgt aaccgttgct ttgcaaccct tggtgcttag ttccctttca
     3241 ccaagcatat tagaaacgta gatagctttt gagagttatc cagacgttaa aggagaaaaa
     3301 atgacaaaat tgactgtaca cgactatgtt cacattgggc tagtagctgc cttgtatgtt
     3361 gtcttaacta ttaccccgcc tttaaatgcg atttcttatg ggatgtatca atttagaata
     3421 tctgaaatga tgaatttctt agctttttat catcgcaagt atattattgc ggtgactctt
     3481 ggatgtatga ttgctaactt ttatagtttc ggactgattg atgtctttgt aggcggcggt
     3541 tcaactctta tttttgtgac cttaggtgtg attttgttta gtaagtatca aaaagattat
     3601 ctttttaatg gcatttttaa taaagctttt gtttactttt ctttcttttt tgcaacttcc
     3661 atgtttactg tagcaatcga actttacttc tttggagctc catttttatt aacatggttt
     3721 acaactgcgt taggagaatt agtatctctg ctcataggat cacttattat tgataagttg
     3781 tctcaaagaa tttcctttta gtaatgcatc atactcaaaa gagttgggtc aatacccttc
     3841 tcttttttat gttgtttgta atgttaaaat cgttctagat agaaatattt aacactttag
     3901 ctaatagtac catatggagt ggccaacgat tacttttgat gttgttatga tatagaaata
     3961 gctcatttgt tatcttttat gataaaataa aagtatgaaa aaacgcataa aagaattaac
     4021 agatcttctt aaccgttacc gttatgacta ttatactaaa gatgccccat cagtttctga
     4081 cagtgactat gataagcttt atcgtgaact ggtgacttta gagcagtcat atcccgagta
     4141 tgtcttgcaa gatagcccaa cgcagcaggt tggagggact attttaaagg ggtttgaaaa
     4201 atatcgacat cagtatccgc tttttagttt gcaggatgct ttttcacgtg aggagctgga
     4261 tgcttttgat aagcgcgtca aagccgaatt tccaaatgcc acctatctag cagagttaaa
     4321 aattgatggt ttatctattt ccttgagtta tgagaatggc ttcttacagg ttggagcaac
     4381 acggggtgat ggcaatatcg gtgaaaatat cacagaaaat atcaaaaaaa taaaggatat
     4441 tcctcaccaa ttgagtgagc ctttaaccat caccgttcga ggagaggctt acatgtctcg
     4501 tcagtccttt aaagcgatca atgaagcacg ccaagaaaat ggggagactg aatttgctaa
     4561 tcctagaaat gctgccgctg ggacactaag gcagctagat acggcagtgg ttgctaagcg
     4621 tgaactagcc acttttttgt atcaagaagc tagtccgaca gctagaaatc aacaaaatga
     4681 ggtcttagca gaattagcag atctaggttt ttcagtaaat ccgtattatc aactcacatc
     4741 ttctatggat gagatttggg attttatcaa aactattgag gcaaaaagag atcaattagc
     4801 ttatgacata gatggcgttg ttattaaagt taatagccta gccatgcaag aagagctagg
     4861 gtttacggtt aaagctccac gctgggccat tgcttacaaa tttccagcag aagaaaaaga
     4921 ggcagaaatc ctttcggttg actggacagt gggacgaaca ggcgtggtca cacctacagc
     4981 gaatctaact cctgtgcaac tagcggggac aaccgtgagt cgtgcaaccc tacataatgt
     5041 ggattatatt gccgaaaaag atatccgtat tggcgataca gtgattgtat ataaggcagg
     5101 ggacattatt cctgctgtct taaatgtcgt tatgagtaag cggaaccagc aagaagtcat
     5161 gcttattcct aaactatgcc catcttgtgg tagtgaatta gttcattttg aagatgaggt
     5221 tgctttgcgt tgtattaatc cgttgtgtcc tagtttgatt cagcgaagtt tggagcattt
     5281 tgcaagtcgt gatgccatga acattactgg gctgggacca gctattgtgg aaaaactatt
     5341 tttagcgggg tttgttcatg atgttgctga tatttaccag ttgaccaaag aaaatttcat
     5401 gcaattagat ggcatcaaag aaaagtcagc tgataaattg ctcgctgcga ttgaggcttc
     5461 taaatcaaat tcagctgaaa aactgttatt tggtctagga attcgtcata ttggctctaa
     5521 agttagtcgc ttaattttag aggtatacgg agatatttct gctcttttaa ccgctaaaga
     5581 agaggagatc gcaaggatag atggccttgg ctcaacgatt gctcaatcac ttactcaata
     5641 ctttgaacaa aaaacggctg cgattttagt agacgagtta aagacagcag gtgtcaacat
     5701 gcattactca ggtcaaaaag ttaatagtga cgcagcgctt tttggtctaa ctgttgtttt
     5761 aactggcaaa ttaaatcagc tgaaccgaaa tgaggccaaa gataaattag aggcattagg
     5821 tgccaaagtt actggaagtg tatctaagaa aacagattta gttattgcag gtagtgacgc
     5881 tggttctaag ctagaaaaag caaagagctt aggaattcgg attgaagatg aagattggct
     5941 acgaaagctt taagtgaggt aatgttatga aaaaacaatt gagagcacgt ctcatttata
     6001 atccaacgtc aggacaagaa cttatgagaa aaagtgttcc tgaagtttta gacatattag
     6061 aagggttcgg gtatgaaaca tctgcctttc aaacaacagc acaaaagaac tctgctctaa
     6121 atgaagcaag aagagcggca aaggcaggtt ttgatttatt gattgctgct ggaggtgatg
     6181 gcactattaa cgaagtggtc aatggaattg ctcccttgaa aaagcgccct aaaatggcca
     6241 ttattccaac aggtaccact aatgattttg ctagagcgtt aaaagtacca agaggaaatc
     6301 cgagccaggc agcaaaactt atcggaaaaa atcagactat ccagatggat attggtagag
     6361 ctaagaaaga tacttatttc atcaatattg ctgcagcagg aagtttgacg gaattaactt
     6421 acagtgtacc aagtcagtta aaaacgatgt ttggatactt ggcttatctt gctaaaggag
     6481 tagaactgct cccaagagtt agcaatgtgc ctgttaaaat tacgcatgac aaaggcgtct
     6541 ttgaaggtca agtttctatg atttttgcag ctattaccaa ttctgttggg ggatttgaaa
     6601 tgattgctcc tgatgcaaaa ctagatgacg gtatgtttac gctcatttta ataaagacag
     6661 caaatctttt tgaaattgta catcttttgc gtttgatttt ggatggtggt aagcatatta
     6721 cagatcgccg tgtcgaatac attaagacca gtaaaatagt cattgaaccc caatgtggta
     6781 agcgaatgat gattaattta gatggcgagt acggaggaga tgctccaatt acattagaga
     6841 acctaaaaaa tcatatcact ttttttgcag atactgattt gatttctgat gaagctttag
     6901 tcttagatca ggacgaattg gaaattgagg aaatcgtaaa gaaatttgct catgaagtag
     6961 aagacttaga acaagaatta gaagaataat acaatagtta ttagctactg acagtttagt
     7021 caaatgcact actattagaa tctaagattt ttaaatcaat tgaggtagtg ttttttgatg
     7081 cgctcaaaga gtcaatttat tcatatgagg ggcccatttg aggtctctct tttttgatca
     7141 tatgcaagaa aatgccatgt tttaggggtg ttattatacc ctttttggaa tcttgttgcc
     7201 tttttgttat aataagagtg taattctatt tttaaaaaga tcttaacaaa gacagactcg
     7261 tttatcaaaa aggttagtat tagtctaaga aagcgtactc agtctaaaga aatctgttga
     7321 cattcttaat tttaaggaat agaatagcaa agtaaaaaaa atttaaagga gtattctact
     7381 atgaatccta ttttcgcatt ggctttggcc tgttttggtg tttctcttgc cgaaggtttc
     7441 ttgatggcaa atctttttaa agcagcatct cgccaaccag aaatcattgg tcaattacgt
     7501 tcattaatga tcttgggtgt tgcctttatc gaaggtactt tctttgttac ccttgttatg
     7561 gccttcattc ttaaataaca gcctatttga taatagaaaa ggagggtgtt agatggaaga
     7621 agctaaaata cccatgctaa agcttggtcc aataaccttt aatttgaccc tacttgctgt
     7681 ttgtattgtc acaattgctg ttatctttgc ctttgttttt tgggcaagtc gccaaatgaa
     7741 attgaaacca gaagggaagc aaactgcttt agaatattta atcagttttg tggatggtat
     7801 tggagaagag cacttagatc ataatctaca aaaatcttat tcgctgttac tctttaccat
     7861 ttttctcttt gtagctgtcg ctaataattt agggttattt actaagttag agacagttaa
     7921 tggctataac ctatggacct cgccaacagc caatctggct tttgaccttg ctctatctct
     7981 ttttattacc ttaatggtac acattgaagg ggttagacgt cgtggcttgg ttgcccattt
     8041 gaaacgtttg gctacaccgt ggccaatgac tccgatgaat ttattagaag agttcacaaa
     8101 tttcttatca cttgccatta ggttattcgg taatatcttt gccggggaag ttgttacagg
     8161 tttgattgtt caactggcca attatcgagt ttattggtgg ccgatcgctt tcctagtcaa
     8221 tatggcgtgg acagcctttt cagtctttat ttcctgcata caggcttttg ttttcacaaa
     8281 actaacagcg acctatctag gaaagaaagt taatgaatca gaagaataag gaaggagaac
     8341 aaacgaatgt caatcacatt tggtgaactc gttgggaatt ttattctagt aacaggttct
     8401 gtaatcgtct tgttactctt gatcaaaaaa ttcgcttggg gtgcaattga atcgatttta
     8461 cagacacgct cacaacaaat ctctcgagat attgatcagg ctgagcaatc acgtctaagt
     8521 gctcaacagt tagaggcaaa aagtcaagct aacctagatg ctagtcgttc acaagcaagt
     8581 aaaatcatta gtgatgccaa agaaattggt caattacaag gtgataaatt ggtggcagaa
     8641 gctactgatg aagcaaaacg cttgaaagaa aaagcgttga cagatattga acaaagcaaa
     8701 tcagacgcta tttcagcagt caaaacagaa atgtctgatt taacggttct tttagcggaa
     8761 aaaattatgg gagccaatct tgataagacg gcgcaaagcc agcttattga cagttatctt
     8821 gatgacttag gagaagctta atgaccaaaa aagaacaggc tcttatcgag caatatgcaa
     8881 aaagccttgt tgaggtggca agtgaacatc attctcttga tgccttacaa gctgatgtgc
     8941 ttgcgatttt agagacgttt gtaacaacta atcttgatca aagtctatca agtctagctg
     9001 ttccgcatgc tgaaaaaata aaattgctaa ccttattaaa gggaaataat tctgtttata
     9061 tgaataattt cttgaatctc attttacaaa acgaacgtga agcttatttg tatcaaatgc
     9121 ttcaggctgt tttaaatgaa attgcaatag tttcaaatca gtatgatgtg acagttacct
     9181 catcactacc tttgactgag gagcaaaaaa gccgtgttcg agcagttgtt gcaaagaaat
     9241 ttgctgtaac agcgggtcgt ttaattgaaa aagtagatcc ttcgcttatt ggaggattta
     9301 ttatcagcgt caataataaa gtaattgata ccagtattcg tcgtcaattg caagcattta
     9361 aaatgaattt aaaatagaaa gtggtgtgac ttttggcaat taatgcacaa gaaattagcg
     9421 ctttaattaa aaagcaaatt gagaacttcc agccaaattt cgatgtcaca gaaactggga
     9481 tcgttaccta tattggtgac ggtattgcac gtgcccgtgg attagataat gccatgagtg
     9541 gtgaacttct tgaatttgaa aatggtgcct atgggatggc tcaaaacctt gagtcaaatg
     9601 atgtgggtat tattattctt ggtgattttt cagcaatccg tgaaggagat gtggttaagc
     9661 gcaccggtaa aatcatggaa gtaccagttg gtgaagctct cattggacgt gtggtaaacc
     9721 cacttggaca accagttgat ggcttgggag atattgaaac aactggcttt agaccagtag
     9781 aaacgccagc tcctggtgtt atgcagcgta aatccgtttc tgaaccctta caaactggat
     9841 taaaagcaat tgatgctttg gttccaattg gtcgtgggca acgtgagttg attatcggag
     9901 accgtcaaac aggaaaaaca tccgtagcta ttgatgcgat tttgaaccaa aaaggtcaag
     9961 acatgatttg tatctatgtg gctattggtc aaaaagaatc aaccgttcgt acacaagttg
    10021 aaacccttcg ccgttacggt gccttggatt atactattgt tgtgacagcg tcagcctcac
    10081 aaccatcacc attgctcttt atcgctcctt atgcgggtgt tgccatggct gaagaattta
    10141 tgtaccaagg caagcatgtc ttgattgtct atgatgactt atcaaaacaa gcggtggctt
    10201 atcgtgaatt gtctctcttg cttcgtcgtc caccaggtcg tgaggcttac ccaggggatg
    10261 ttttctactt gcatagccgt cttttggaac gctctgcaaa agtttctgat gatttaggtg
    10321 gaggttctat cacagctcta ccatttatcg aaacacaggc cggagatatc tctgcatata
    10381 ttgcgactaa cgttatttcg attacagacg gacaaatctt cttgcaagag aacctcttta
    10441 attcaggaat tcgtccagcg attgatgcag gatcatctgt ctcacgtgta ggtggttcag
    10501 ctcaaattaa agcgatgaaa aaggttgctg gtactcttcg tttggatttg gcttcctatc
    10561 gtgaattaga agcctttacc caatttggtt cggaccttga tgctgcaaca caagcaaaac
    10621 ttaatcgtgg tcgccgtacg gttgaaatct tgaagcaacc attgcataaa ccgttacctg
    10681 ttgaaaagca agtcgttatt ctttatgccc ttactcatgg tttcttagat gatgttcctg
    10741 tagatgatat tttggctttt gaagaagctc tttacgacta ctttgacgtt cattacaatg
    10801 atttatttga aactatccgt accacaaaag accttcctga agaagctgcc cttgatgccg
    10861 ctattaaagc gtttaaagaa cactcaaact ttaagtcata gagggaggat actagtatgg
    10921 caggctctct aagtgaaatt aaagctaaaa ttatttcaac tgagaaaaca agtaaaatca
    10981 ctagtgccat gcgtatggtt tcttctgcaa aacttgttaa atctgagcaa gcagctcgtg
    11041 attttcaaat ttacgcctca aaaattcgcc agattactac agatttattg aaatcagaat
    11101 taaccatagg atctgataat ccgatgttgg tgtctcgtcc tgtcaaaaaa acaggctata
    11161 ttgtcatcac gtctgataaa ggacttgttg gaggttacaa ttcaaaaatc ttgaaatctg
    11221 ttatggatat gattacagag taccatgctg atggtgacta tgaaatcatt tctattggta
    11281 gtgttggttc agacttcttc aaagcaagag ggatgaatgt ggcttttgaa cttcgtggac
    11341 ttgcggatca accaagtttt gaacaagtga gacaaatcat ttcacaatcc gttgacatgt
    11401 ttgtcaatga gatttttgat gagttatatg tctgttacaa tcaccatgtg aatagcttaa
    11461 ccagccaagt ccgtgttcaa cagatgttgc caatttctga cttggttgct gatgaagcag
    11521 cagaagaagg tgttactggg tttgaattgg aacctaatcg tcatgatatt ttagatcaat
    11581 tgttacctca attcacagaa agtttaattt atggggctat cattgatgct aaaacggctg
    11641 aacatgcagc aggtatgact gctatgcaga cagcgacaga taacgctaag aatgtcatta
    11701 acgatttgac catccaatat aaccgagctc gtcaagcagc aattacacaa gaaattactg
    11761 aaattgttgc aggagcaaat gcactagaat aaaaaatgac gctgactagc gattaggtta
    11821 ttgtcagcat tcaattttat caatcgagat aaaatgactt atgggacttt tctattgaaa
    11881 gactgctctt ttcagtatga aagaagttag caagactaac taagccctga ctatcgaata
    11941 taaggagaaa tgaatgagct caggcaaaat tgctcaggtt gttggtccag tagtagacgt
    12001 tatgtttgct agtggggaca aacttccaga gattaataat gcattgattg tttataaaga
    12061 tagtgataaa aagcaaaaaa tcgtccttga agttgctctg gaacttggtg acggtatggt
    12121 gcgaacaatc gctatggaat caactgatgg gcttacacgt gggttagaag ttcttgacac
    12181 tggtcgtgcg attagcgtac cagtaggtaa agaaactttg ggacgcgtct ttaatgtact
    12241 tggagaaacc attgacttgg aagaaccatt tgcagaagat gttgaccgtc agccgatcca
    12301 taaaaaagca ccatcgtttg atgaattatc aacatcatca gaaattcttg aaactggtat
    12361 caaggtaatt gaccttcttg ccccgtacct taaaggtggt aaagttggac tttttggtgg
    12421 tgccggagtt ggtaagactg tccttatcca agaattaatc cacaatatcg cccaagaaca
    12481 cggaggtatt tcagtattta ccggtgttgg tgagcgaaca cgtgaaggaa atgaccttta
    12541 ctgggaaatg aaagaatcag gcgttattga aaaaaccgcc atggtttttg gtcagatgaa
    12601 tgagccgcct ggggcacgta tgcgtgtagc ccttactggt ttaaccattg ctgagtattt
    12661 ccgtgatgta gaaggccaag atgttttgct ctttattgat aatatcttcc gtttcactca
    12721 ggcaggttca gaagtatcag ccctcttagg ccgtatgcct tctgctgttg gttaccaacc
    12781 aacccttgct actgaaatgg ggcaattgca agaacggatt acatcaactc aaaaaggatc
    12841 tgttacatct atccaagcga tttatgtacc agcagatgat tatactgacc ctgcgccagc
    12901 aacagcattt gctcacttgg attcaacaac taaccttgaa cgtaaattga ctcaaatggg
    12961 tatttatcca gccgttgacc cattggcttc aagttcacgg gccttatcac ctgaaattgt
    13021 tggtgaagag cactatgctg tggcaacaga agttcaacgt gtgcttcaac gctaccgtga
    13081 attacaagac atcatcgcta ttttggggat ggatgaattg tcagatgaag aaaaaacatt
    13141 ggttggacgt gcgcgtcgta tccagttctt cctctcacaa aattttaacg ttgctgaaca
    13201 atttaccggt cttcctggtt cttacgtgcc agttgctgag acagttcgcg gctttaaaga
    13261 aattcttgaa ggaaaatacg atcaccttcc agaagatgcc tttcgttcag ttggtccaat
    13321 cgaggatgtg attaaaaaag ccgaaaaaat gggcttttag tgaggtgatg ccatgacaca
    13381 aatgactgtt caagtggtaa cgccagatgg aatcaaatat gatcaccatg ctaaatgtat
    13441 ttctgtaaca acaccagatg gggaaatggg gattttacct aatcacatca atttaattgc
    13501 acccttgcaa gttcatgaaa tgaaaattcg ccgaggtggt gaagatgaaa aagtggactg
    13561 gattgctatc aatggtggta ttattgaaat caaggataat gtggttaccg ttgtcgctga
    13621 ttctgcagaa cgtgaccgtg atatcgatgt cagccgagca gagcgagcaa aactaagagc
    13681 agaacgtgaa attgcacagg ctgaaacgac ccacaacatt gatgaagttc gtcgtgccaa
    13741 ggttgccttg cgccgcgctt tgaatcgtat caacgttagt aaaaaataag tggtgttgat
    13801 aaaagacaag gataagtaag aaaggttttt aacctattat tttgtttgct gagtcttttt
    13861 aacatcagtt tctgagagtt tgaccatact accttctcta aacacgttat tattgttgag
    13921 aggagttata tgggggaact cttttttgta tccacgttgt tagtagaaac tcagtaatga
    13981 cgcttaatgt attttcaggc tatcaactta gtcagagagg atgtcaattt aattgataag
    14041 ttggacggat caagatggta tgttataata atcctatgga attattaatg aagatcattg
    14101 ccttttcgtg tcatttattt tttattggcc ttagttacca attattgatt agtgtgattg
    14161 actggacaaa atttagtcat tatcatccag aaaatcttgg ccggctacga ctttttgtct
    14221 ttttattagc tactgcccta gggtatttgg tgagccattg tatgctggaa ttgcttcaaa
    14281 ttagccagac gctctttttt gagttccgct aatactggaa taaaagaagt atggtataat
    14341 tttactacga ctaagagata gttaagggag aaaattgtgg ataaaataat cattgaaggt
    14401 ggtcaaacac gcttagaagg agaagttgtt attgagggtg ctaaaaacgc agttcttcct
    14461 ttactggcag catctatttt accttcaaaa ggaaaaacaa ttcttagaaa cgttcctatt
    14521 ctatctgatg tttttactat gaataacgtg gtacgtggtt tagatattcg tgttgatttt
    14581 aatgaagcag ctaacgaaat cacagttgat gcctcaggac acattttaga tgaagcccct
    14641 tacgagtacg tgagccaaat gcgtgcctct attgttgttt taggacccat tttggcacgt
    14701 aatggccacg caaaagtatc aatgcctgga ggctgtacga ttggcagtag gcctattaat
    14761 ttgcacttaa aaggattaga ggctatgggg gcaacgatta ctcaaaaagg tggagatatt
    14821 actgctcaag cagatcgact gcaaggtgcc atgatttata tggacttccc atctgtagga
    14881 gctacccaaa acttgatgat ggctgccact ttagctgatg gtgtgaccac tattgaaaat
    14941 gctgctcgtg agcctgaaat tgttgattta gctcaatttt tgaataaaat gggagctaga
    15001 attcgtggtg ctggaacaga gacgttaacg ataacaggcg tcacgcattt acgaggcgta
    15061 gaacatgatg ttgttcaaga tcgtatagaa gccggtacat ttatggttgc agcggcaatg
    15121 acttcaggta atgttttgat tagagatgct gtttgggaac ataatcgtcc attgatttca
    15181 aaattaatgg aaatgggtgt ttctgtaaca gaagaagaat acggtattcg cgttcaagca
    15241 aacacgccaa agctcaaacc tgtaacggtt aaaaccttgc ctcatccagg ttttccaaca
    15301 gacatgcaag ctcaatttac agctttaatg gcagtggtca atggagagtc tactatggtt
    15361 gaaactgtct ttgaaaatcg atttcaacat ctggaagaaa tgcgacgcat gggcttacaa
    15421 tctgaaatcc ttagggaaac tgccatgatt catggtggaa gacaactgca aggtgctcct
    15481 gtgatgtcaa cagatttacg tgccagtgct gcccttatct tgacagggat tgttgctcaa
    15541 ggagttacaa tagttaataa tttggttcat ttagatcgtg gctactatca atttcatgaa
    15601 aaattagcaa agcttggagc tactattagt cgtagcagtg aggtctagct tatgacaaat
    15661 ggtttaaaat atgtcttaga acaaatgtta ttacttttca taattgctgc cttggcgtgt
    15721 ctttttttag ccattggact tatgattggt tatagtttta tgggagatgg ccatagtcca
    15781 tggcatattt tatctatgga taagtgggca gagttagtga ataaatttac tggaaagtag
    15841 gccttgccta ctttttatag catctgagag gaagtatgtt aacaaggaaa aaacgaaaaa
    15901 gggtgcaaga gcaaaaggtt cctaaaagag ccaacgcttt acttgctaag tcaacccggc
    15961 aatatcaaga ccgttcataa cttaagaaag gttaccgttc atttaagcca gcaggttggc
    16021 accgattgca ccacttaaaa ggaagttatg atcacgcggt tgatagaggg catttgctag
    16081 gttatgcttt agttggtgga ctgaaaggtt ttgatgcttc tactggtaat cctgataata
    16141 ttgccactca actaagttgg gctaatcaag caaataaacc ctactcgaca ggtcagaatt
    16201 actatgaagg tttagtacgt cgtgctttgg ataaggggca tcgcgtccgc taccgtgtca
    16261 ccctacttta tgatggtgat aatctactag ccagcggtag ccatttggag gctaaatcct
    16321 ctgatgacag cttggctttt aacgtgtttg ttccaaatgt ccaagcagga ttgaccgttg
    16381 actatcggac gggacaaata gctgtcaaca attgcctttt gcttatctct tcagttgctg
    16441 tgattttatc atacagtgat aaaagat
//
LOCUS       DAUCLR010000034        15780 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.034, whole genome shotgun sequence.
ACCESSION   DAUCLR010000034 DAUCLR010000000
VERSION     DAUCLR010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15780
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.034"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(26..379)
                     /locus_tag="VGT87_000949"
     CDS             complement(26..379)
                     /locus_tag="VGT87_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ3813820.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            884..1306
                     /locus_tag="VGT87_000950"
     CDS             884..1306
                     /locus_tag="VGT87_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEQ3813821.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYLLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGISVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            1303..1533
                     /locus_tag="VGT87_000951"
     CDS             1303..1533
                     /locus_tag="VGT87_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ3813822.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            1991..2194
                     /locus_tag="VGT87_000952"
     CDS             1991..2194
                     /locus_tag="VGT87_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEQ3813823.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQVKRKQFEKIIDTLNAI"
     gene            2276..3493
                     /locus_tag="VGT87_000953"
     CDS             2276..3493
                     /locus_tag="VGT87_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEQ3813824.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYTDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGARSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLNAVLDDDTVPKTVLTGEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            complement(3868..4536)
                     /locus_tag="VGT87_000954"
                     /pseudo
     CDS             complement(3868..4536)
                     /locus_tag="VGT87_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSsu5 family transposase"
     gene            complement(4532..4894)
                     /locus_tag="VGT87_000955"
                     /pseudo
     CDS             complement(4532..4894)
                     /locus_tag="VGT87_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibD"
     gene            complement(5005..5412)
                     /locus_tag="VGT87_000956"
     CDS             complement(5005..5412)
                     /locus_tag="VGT87_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEQ3813825.1"
                     /translation="MVLNRFCRLRNEYRNFRVDRIKSICIEEELCQSHDGSLEQILKQ
                     MLSYKKLYNVILRAEKGETYNSIKNRYSLGFLEETDLGSKMEIEFQTDSFEILSKQLI
                     EYGSGIEIVQPDELKCITRKHLAQITNHCLNLI"
     gene            complement(5385..5957)
                     /locus_tag="VGT87_000957"
     CDS             complement(5385..5957)
                     /locus_tag="VGT87_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEQ3813826.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
     gene            complement(6233..7027)
                     /locus_tag="VGT87_000958"
                     /note="aph(3')-IIIa"
     CDS             complement(6233..7027)
                     /locus_tag="VGT87_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEQ3813827.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(7120..7662)
                     /gene="sat4"
                     /locus_tag="VGT87_000959"
     CDS             complement(7120..7662)
                     /gene="sat4"
                     /locus_tag="VGT87_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HEQ3813828.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            complement(7659..8567)
                     /locus_tag="VGT87_000960"
     CDS             complement(7659..8567)
                     /locus_tag="VGT87_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HEQ3813829.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            complement(8600..9334)
                     /locus_tag="VGT87_000961"
     CDS             complement(8600..9334)
                     /locus_tag="VGT87_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEQ3813830.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            complement(9315..10184)
                     /locus_tag="VGT87_000962"
     CDS             complement(9315..10184)
                     /locus_tag="VGT87_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEQ3813831.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            complement(10199..10423)
                     /locus_tag="VGT87_000963"
     CDS             complement(10199..10423)
                     /locus_tag="VGT87_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ3813832.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            complement(10566..12110)
                     /locus_tag="VGT87_000964"
     CDS             complement(10566..12110)
                     /locus_tag="VGT87_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEQ3813833.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKLIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKTRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHLPQ
                     YYVENSHEAIIPKELFLQVQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRTYWNIHGRKEFVWRCVTRIEQGPGVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            complement(12130..12546)
                     /locus_tag="VGT87_000965"
     CDS             complement(12130..12546)
                     /locus_tag="VGT87_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HEQ3813834.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            complement(12547..12843)
                     /locus_tag="VGT87_000966"
     CDS             complement(12547..12843)
                     /locus_tag="VGT87_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HEQ3813835.1"
                     /translation="MSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTNSASGVLRAN
                     EIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLKEKVVR"
     gene            complement(12880..13044)
                     /locus_tag="VGT87_000967"
                     /pseudo
     CDS             complement(12880..13044)
                     /locus_tag="VGT87_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898188.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
     gene            complement(13356..13688)
                     /locus_tag="VGT87_000968"
                     /pseudo
     CDS             complement(13356..13688)
                     /locus_tag="VGT87_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701960.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
     gene            complement(13681..15309)
                     /locus_tag="VGT87_000969"
     CDS             complement(13681..15309)
                     /locus_tag="VGT87_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEQ3813836.1"
                     /translation="MIKKVRIKNFKCFKDWFELDLNGGINILVGNNEEGKSTILEAIH
                     LALTGFYRGSYINRELSQYLFNREVVDEYLTNINSNSPCALPEIIIEIYFSELDDANL
                     EGSGNGLGLKEEIGFAFKVLFDNSYEDEYKLFMEKGELSSLPIEYYKSEWLGFNRENI
                     TTRSIPSKSFLIDNSSYKYKDGSDQYISNMIRGTLDNEDLISISKAFRETKDDFSKNE
                     SVKNINEKLSGSKSNLGKKVSLGVNLGSNFNWESSMITEFDSIPYSNAGRGLQCLLKT
                     ELALNNINTNKDKIILIEEPENHLSYSNMNNLINILQENSNKESSQIIISTHSSFVLN
                     KLGLENLILLSNKKSSKITNLSSGTEKYFKAVSGYDTLRVLLCDKAILVEGPSDELII
                     QRAYKDKYGKLPIFNGIDIISVGTSFLRFLELGDKLDLNLYVVTDNDGDVEALMRKYK
                     DYEYNEKIKIYYDEKTYSGDMDKFNYNTLEPCILRANDLKTLNSIFNTNKTDSDSILK
                     YMEKNKTNCAVSIFESESKIQYPEYINRVIEDVE"
     gene            complement(15332..15538)
                     /locus_tag="VGT87_000970"
     CDS             complement(15332..15538)
                     /locus_tag="VGT87_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003146610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ3813837.1"
                     /translation="MELSYKRLFKKLIDLDMQHNELMEKANVSRSTFYKLKNGENVTT
                     DVLLRICDALDCDISEIVECIKND"
BASE COUNT         4714 a         3143 c         2523 g         5400 t
ORIGIN      
        1 ttccgttatg tatggattct tttaattagt cttcgatgtt tcttgcttcg ttgataccgc
       61 tggctaaaga ttccattaag gatagttctt tgtctgtaaa gctatccatg tatttctcta
      121 tctgtaatcg tcgggtgctt tttaccaagt tattagcagg taagaaaaat tcatcaacgg
      181 aaacatgaag taacgataca aggtcataaa gaacttgtat gctggggtgt tgccctttat
      241 tttcaatatt agttaagtac cgtgggtcaa tttcaatcaa tgctcccact tgttcacgag
      301 ttaaacctcg tttcaatcga gcttctttaa tggctaaacc aaaggctcta aaatcatatt
      361 tatcttcttt tttacgcata gtagaccacc tctatacatt ttattgttcc tactgaatta
      421 aaaacaggta tagaaaaacg tgttatatgg tttataggtt tatatttaat aaaaagcact
      481 actaaacgcc aataaaaaaa accgttatat ggtagtgcta tttacgctgt taaaatattg
      541 tatattactt ccaaatggcg gtttgttgga ggtcaacgtc gccatgaagt acatcatata
      601 caataaattt ccttacattg ggttcttgtc aaaaaaagtc gtctatctgc aatagataag
      661 tacgtccacc aatgtggttt tataaatcat atagatagaa taacagaagc atgtaaacag
      721 agaaataaat ctgtttatat gcttttttgg ctattcagaa cttttttaca aagtttattt
      781 atcagtaatg caacaaatcc ccctttcaca ttgggactaa gagtgaaagg agataaacga
      841 gcaaggctca cttcctttcc tagacagaaa gggggtgaga aacatgaaac catcttcttt
      901 tcagaccaca atagaaaatc agtttgacta tatctgtaaa cgtgctatgg aagacgagcg
      961 aaagaattat ctgctttatc tttcaaggat cgcaaagcgt gaagtgtcct tttctgatgt
     1021 tggcgattat cttgttagcc agtttgcgac aacagataac tattcaactg actttcagat
     1081 ttttacactc aatggaatat cagttggtgt tgaaaatgat ttattgagtg aagcattacg
     1141 tgagttgcca gacaagaaac gtgaaattct actgctgttt tactttatgg acatgagcga
     1201 ttcagaaatt gcagacctgt tgaaattgaa ccgttctact gtctatcggc atagaaccag
     1261 tggactagcc ttaatcaaaa agtttatgga ggaatttgaa gaatgaaaac acaatatcct
     1321 atgattccct ttcctctcat tgtaaaggca acagatggcg atacagaagc gattaaccag
     1381 attctacatc attacagagg gtacataacg aaacgttccc tacgacttat gaaagatgaa
     1441 tatggcaatc aaagtatggt cgttgatgaa gtcttacgtg gaagaatgga aaccagactg
     1501 attacaaaga ttctgtcatt tgaaattaag taataacctc tctcttttcg tggaagcgtg
     1561 ccatactatt ccacgcttcc cgaacaggga ggtttgttat tccatcaaag catattgagc
     1621 tttcaatgtg ttttgatagg ctaacgagcc attgttcttt gaaaactgaa taaaagtaat
     1681 tgaatacgtt tcgataagaa aagagccaac ggagctaacc gccatgacct atcttctaaa
     1741 gatagcgagc gattcatgtt agtgttccga aaaacaatct ttagcaggat tgccagcgac
     1801 gactttctta tcgtgataat gatactccca tacagtcaat agtccgagcg ttaaaagcgt
     1861 cgcaggcaat gagtatggct acatgagaac catgcagggg tggaactccc gtgagctttg
     1921 ctagagctgt tcgattgctt gtaaaacaac ttttatgaaa tccaataagt gatttggaaa
     1981 ggaggatttt atgaagcaga ctgacattcc gatttgggag cgttataccc taactattga
     2041 agaagcgtca aaatattttc gtattggcga aaacaagctg cgtcgtttgg cagaagaaaa
     2101 taaaaatgca aactggctga ttatgaatgg caatcgtatt caggttaaac gaaaacaatt
     2161 tgaaaaaatt atagatacat tgaatgcaat ctagcgtagc caaagggtct tgtatatgat
     2221 aaaatagtat taagtcgtat cagggctctt tccataatgg aaaggagcaa atgccatgtc
     2281 agaaaaaaga cgtgacaata aaggtcgaat tttaaagact ggagagagcc aacgaaaaga
     2341 cggaagatac ttatacaaat atacagattc atttggagaa ccgcaatttg tttactcgtg
     2401 gaaacttgtg gctacagaca gagtaccagc aggaaagcgt gattgtatct cacttaggga
     2461 gaaaatcgca gagttacaga aagacattca tgatggtatt gatgttgtag gaaagaaaat
     2521 gacactctgc cagctttacg caaaacagaa cgctcaaaga ccaaaggtta gaaaaaatac
     2581 tgaaactgga cgcaaatatc ttatggatat tttgaagaaa gacaagttag gtgcaagaag
     2641 tattgatagt attaaaccat cagacgctaa agaatgggcg attagaatga gtgaaaatgg
     2701 ttatgcctat caaaccatca ataactataa acgttcttta aaggcttcat tctacattgc
     2761 gatacaagat gattgtgttc ggaagaatcc atttgacttt caactgaatg cagttcttga
     2821 tgatgatact gtccctaaga ccgtactaac aggagaacag gaagaaaaac tgttagcctt
     2881 tgcgaaagct gataaaacct acagcaaaaa ttatgatgaa attctgatac tcttaaaaac
     2941 aggtcttcgt atttcagagt ttggtggttt gacacttcca gatttagatt ttgagaatcg
     3001 tcttgtcaat atagaccatc agctattgag agatactgaa attgggtact acattgaaac
     3061 accaaagacc aaaagtggtg aacgtcaagt tcctatggtt gaagaagcct atcaagcatt
     3121 taagcgagtg ttagcgaatc gaaagaatga taagcgtgtt gagattgatg gatatagtga
     3181 tttcctcttt cttaatagaa agaactatcc aaaagtggcg agtgactata atggtatgat
     3241 gaaaggtctt gttaagaaat acaacaagta taacgaggat aaattgccac acatcactcc
     3301 acatagtttg cgacatacat tctgtaccaa ctatgcaaat gcaggaatga atccaaaggc
     3361 attacagtac attatgggac atgctaatat agccatgacg ctgaactatt acgcacatgc
     3421 aacattcgat tctgcaatgg cagaaatgaa acgcttgaat aaagagaagc aacaggagcg
     3481 tcttgttgct tagtagtaca aatgaattta ctacttattt accacttctg acagctaaga
     3541 catgaggaaa tatgcaaaga aacgtgaagt atcttcctac agtaaaaata ctcgaaagca
     3601 catagaataa ggctttacga gcatttaaga aaatataaaa agataattag aaatttatac
     3661 tttcctgaat aattcatagc tctaaattta acctgttttt ttgaacaaat tgccaatttt
     3721 taaattaggc agatactttc ttccaaaata atgtgttttc gaacaggatg cctacggaag
     3781 aagatcataa taaaaattat ttgtctattt aagggcagac gaatcccttg aaaaagaatc
     3841 ggttaaaaaa tttgtgtgat aactagtcta aatccattgt ttggatcgcc gcaggcgttc
     3901 aaaaacctta taaaattcag tttgatacac atgataactt gacagtttga gatagacctg
     3961 tctcccagtc tgaacaagtt taccggctac tttaagcaat atcagccgga tggaatgaat
     4021 cgtcatgccc tggttcactt tatcaaaacc aatgcttttt aaaaaattaa cgaggttgta
     4081 tgcaaggaca ctgatcatca ttctgacatg attttctaag aaacgcgggc tatctgtttt
     4141 atcgaagtaa aagccacctt tcgcttcttt gatgaaattt tccattgttc ctcgcttgcc
     4201 gtaaagagaa aatatgactt caggcgagac attgtcggat agattagtca caataaacgc
     4261 atgatgaaat agcaattctc ctccttcacg gacggagcgt atacagacac gacgaggttt
     4321 cgaccaggat tgtgcttgat aaggtagtga aaaatactgc acttctcgct cttcccattc
     4381 ttgattgtcc ccgtaaagaa cagaatgttc agctaactga cttaacctcc gattgctctt
     4441 taatcggata acgtactggc tttcagttgc ttcacaagac tcatacacct ctggtgtggc
     4501 gaagccgctg tcaccccgaa ccaatatctc ggtatgggta atgcctttcc tccaaataat
     4561 tcaaatattc cttcggagtg gcagttgata ccagaatgac aatttccttc agatatccaa
     4621 gatttcttaa gcaatgaaga cttctcaaga tacctctgct gtcaggtatg actcctattg
     4681 gtctggggtc ctccgggctt gttatgattt tctcaaaatc agcctctgtt tcagcaggat
     4741 atttttcaaa tgcagtaaaa acagtattgg acccaaacag caccgcatcc gaatgaattc
     4801 ccccggcaac ctggtagtaa acttccggat tatcaaagcc tttaatgcgg ccatcaaggc
     4861 taatttgtgt gtgcataatt atctttggtt tcatattttt caatcctcct ttttgatagt
     4921 agaatatcat aatacactga cagctgtatg tcagcagggg atatgaaacc ttacaaaaaa
     4981 tacataacag accaatcgct ttctctaaat caagtttagg cagtgattcg taatttgtgc
     5041 caggtgttta cgggttatgc attttaattc atcaggttgg actatttcaa tgcctgatcc
     5101 atattcaatc agctgcttgc tcaatatctc aaaggaatct gtctggaatt cgatttccat
     5161 tttacttccc aaatcggttt cttcaaggaa gccgagagaa tacctgttct tgatagaatt
     5221 gtatgtctcc cctttttcag ctctaagaat tacattatat agttttttat atgaaagcat
     5281 ttgttttaat atttgttcca aagagccatc atggctttga cagagctctt cttctatgca
     5341 aatgctttta atccgatcca cacgaaaatt cctatattca tttcttagcc tgcaaaaccg
     5401 attaagtacc agttctgttc ataaaacccc agtgatatag gctctatcat tctgctttcc
     5461 ggttcttgcc ctcctgatgc aggatattga attgatatta cccttttatt gcagattgca
     5521 gtctgaattg ctgcaataac gttatccgcc aatgtatttt tctgtccaga tgtgctgtac
     5581 acgttaatac tgttctccaa ctcctgtgca tagttttttt caccatgctt caaaatagat
     5641 ttgattttat acatagctga atcaaaatcc tttataaatg attcatctcc atggcaattt
     5701 aaaaatttcc ctgctgtaat caatgcaccc acttccgccg ctgtaaacat taccggcgga
     5761 agtaggaagc cctctacaag aaaatatcct tttccggcct cagatccaat tggtattccg
     5821 gcttcttcaa gtgtccggat atctctgtaa actgtcctta agcttatatt aaatcgctgt
     5881 gcaatttctt tggcaggaat tatttttttt gattgcagct gaataagaat agaggttact
     5941 ctgttaatcc tgttcaaatt ttattcctcc tctgtgactt ccataaaaag tatatcctat
     6001 gcgttcttaa gctagctgct gttagtgctt ctcattgcac cctggttata aaagccatac
     6061 tcacaaacct cttcaccaaa actctttaca tctctaatat agaactctat ctagcgaact
     6121 tttagaaaag atataaaaca tcagagtatg gacagttgcg gatgtacttc agaaaagatt
     6181 agatgtctaa aaagcttgta gttaaagctt tttagacatc taaatctagg tactaaaaca
     6241 attcatccag taaaatataa tattttattt tctcccaatc aggcttgatc cccagtaagt
     6301 caaaaaatag ctcgacatac tgttcttccc cgatatcctc cctgatcgat cggacgcaga
     6361 aggcaatgtc ataccacttg tccgccctgc cgcttctccc aagatcaata aagccactta
     6421 ctttgccatc tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt
     6481 cctcttcggg cttttccgtc tttaaaaaat catacagctc gcgcggatct ttaaatggag
     6541 tgtcttcttc ccagttttcg caatccacat cggccagatc gttattcagt aagtaatcca
     6601 attcggctaa gcggctgtct aagctattcg tatagggaca atccgatatg tcgatggagt
     6661 gaaagagcct gatgcactcc gcatacagct cgataatctt ttcagggctt tgttcatctt
     6721 catactcttc cgagcaaagg acgccatcgg cctcactcat gagcagattg ctccagccat
     6781 catgccgttc aaagtgcagg acctttggaa caggcagctt tccttccagc catagcatca
     6841 tgtccttttc tcgttccaca tcataggtgg tccctttata ccggctgtcc gtcattttta
     6901 aatataggtt ttcattttct cccaccagct tatatacctt agcaggagac attccttccg
     6961 tatcttttac gcagcggtat ttttcgatca gttttttcaa ttccggtgat attctcattt
     7021 tagccattta ttatttcctt cctcttttct acagtattta aagatacccc aagaagctaa
     7081 ttataacaag acgaactcca attcactgtt ccttgcattc taaaacctta aataccagaa
     7141 aacagctttt tcaaagttgt tttcaaagtt ggcgtataac atagtatcga cggagccgat
     7201 tttgaaacca caattatgat agaatttaca agctataagg ttattgtcct gggtttcaag
     7261 cattagtcca tgcaagtttt tatgctttgc ccattctata gatatattga taagcgcgct
     7321 gcctatgcct tgccccctga aatccttaca tacggcgata tcttctatat aagcgtaccg
     7381 gttccaattt tttcgcagtt taacttttcc gacgcattta tcgtcttggt agtaaagata
     7441 tattatctta tcagtattgt caatatattc aaggcaatct gcctcctcat cctcttcatc
     7501 ctcttcgtct tggtagcttt ttaaatatgg cgcttcatag agtaattctg taaaggtcca
     7561 attctcgttt tcatacctcg gtataatctt acctatcacc tcaaatggtt cgctgggttt
     7621 atcgatatct ttcaggtgcc ctgctttcat ttctgtaatc actgttcccg cctctcttct
     7681 atatcagcgg catatgtgct atccaggcag ccggttttac cagtgtattt tttatacatg
     7741 tccctggtat attttgttat attcctatca tactccggat aggcataatg aagcctttcc
     7801 gccacctcac cggataccgc cctgaacaat tgatggcata gaaataatgc ttcccatatg
     7861 ttttcatagg aatccatccg gtaggtggac aaaagtttct cccacaaatc ctcggatata
     7921 tacctttcaa taaacttata gttcttgcct acacttaatt taaagcctgt ttcgatgccg
     7981 accttccatg atatcattct cagcagctca tggcgaacaa tctgattaaa atgatcaata
     8041 gcaaataaaa tttccttacg gcacaatcct ttaataacat aaggtgttac attccaaaat
     8101 tcattgcagc aatcatcata ctcccttgcg cttggctttc ttacatgata atctatatca
     8161 gtcggaacta tgtccctttt aattctacaa tctttatcaa ttagaacctt tattaattta
     8221 tcgcccttta ggtaattatc taactcttcc aagggcaata aggtaagatc aattttattg
     8281 taatcatcaa atagcataag ataggaaaat cccttttctt caggtgggaa taattccata
     8341 tcctccggct tttgcatcat tattatattc ccaaattgat taagccagtc atcattagat
     8401 ataaacggtt ctatatcact tacaaaatat gtaatatcat aatcctgaaa ttcatcttta
     8461 ggtatattaa tatttgcgcg tgacccctca agggtcacaa ttcgaatacg ttcatcctgt
     8521 tctgctaaag aaagtactaa atccatcatt tctttttctg atctcattta catactcctc
     8581 gtttattttt tctatattat tacatcttct tttttgccga tacaatcagc atcattgggc
     8641 gtcgcatttc atccgccatc cccggaatat ccatcatgtt ctctggcggc tgtggctcca
     8701 caatctgatt tattataaaa ctatttgaaa gcagtgtatt tagatatgtg gtcagtgttc
     8761 tatgatattt tgtaaccttt tcttccaaaa acatagctgt ccgtttgccc tcataataat
     8821 aattgtccac cgggaaatgc agtatttctc ctttttcgtt ataataccag tcttgtgttc
     8881 catgagcagt aaaaacagga tgttcaactg taaaaactaa attgccacca gccttcagca
     8941 tcctatatat cttttttatt aaattctcat agtctgctac ataatgaaac gcaagcgaac
     9001 ttagtattac atcaaagctc tcctctggga aatccacatc ttctatggca cagcattcat
     9061 attcaatctg tggaaaatgg gtttttcctt ttgctacttc gagcatttta tgagaaatat
     9121 caacacctac tacagaggaa gcaccgtttt ccatcgcata tatacagtgc catccatagc
     9181 cgcatcctaa atcaagcaca cgcttaccct taaaatcagg tagcatcttt ttcaaagtct
     9241 cccattctcc cgcaccagcc agtcctttct gcgagcgact catttgactg tatttttgaa
     9301 aaaatatatt atcatcatat ttgttttctt tcatctgaac tctcctcgtt taaaaagtta
     9361 tttatctccg atacaatttc attcacttct ttataaagtt tctcggtcat atcgtagcat
     9421 tcaaaaagag aaagaccgag tacttcaaaa atatgattaa ctctttcggc atatttttca
     9481 ggtttatatt caaaagtttc aagcagtttt atagctttct tttcgttaat gcaataagca
     9541 ttattacatg caaatagtac ttgatttaag catgaaataa tacgaaaaac atggcctgca
     9601 atataatatt tatcctctac ccccgcattt gcttttacaa acattaaaga gaattcagct
     9661 tcaaatataa aaaagttcat caagcttttc tttagagcag tagggtaaat ttctgcctgc
     9721 ttttttaatt cgcataagct ttcgttctta gcatatagta ttttactaat cgctaattct
     9781 ccacgataca ttgcacttat ataaccatgg ggatgcccgg tctgataatt ggcagtaaca
     9841 attccttgct ccgtatcttt gattatttgt tctacccgtt ttatatcacg taaaatcaag
     9901 tcaacatgat acccatttat aaataaccat ccgccgccat taatccaatc accccacgct
     9961 ccgggaggca caacaaggtt gtttctattt ttatcatcca attctgtagc aatttgatta
    10021 atcgctgtca ggtcaaatga gtctgaattg taatagattc cgatatctat atcagaattt
    10081 tctgtatggg tgcctcttgc acgtgagccc cctaatacga tgccttctat aaaaggcaaa
    10141 gatgataatt tctctgttac tatttgaata acattaccta ccatataaac atcctccttt
    10201 attcgttaga tatttttatt tcttctccat ctggtaaaat aaatgcctgc acaaacttga
    10261 cacctagcac atcagcaatg gccttcaact catccaaggt tactgtttct cgttgtaatt
    10321 ttttattgaa attctgtgga gtctggccaa tacgtctagc aagttcggaa acacttatat
    10381 tcatttgctc acacagttct ttaatcattt ctgccgtagt catacagcac ctccaagttt
    10441 acgctaagaa tattataaac catttggtta ataaacacaa tagttattaa aatattatta
    10501 taatcagcaa gcaaaagaaa acccacaact ctactgtaga gtcatgggct atttacttct
    10561 ggttttcata tttccatttc gatgccagat ttgaaggcta tgacgaagtg gccttcatag
    10621 actgtaacgc tctggattat cttccttaca agcttatcat catattctaa ggtgcggaat
    10681 ttgttcttgc ggataaattc aatcagctca ttgattcgct cgttctcgcc actgagggag
    10741 gcatcttcta ctaaaaggat ctgtcgcttg tctctcagct catcaatctc atctgctaag
    10801 tggtcatagt cttgaccctt gttcgccagc ttgattagtt ctttttgttt ttcctctagt
    10861 gagttgttaa tctctgaaat ttggtactct gtcgtttcgc caatcaccgc atgaatattt
    10921 tcttccagtg tctttatcat gttattgcca cctgcaagta gcttattaat cgcagtcatt
    10981 actgcaccat agagttcatc ttcttttacg gttcggttct tacagacccc aggaccctgc
    11041 tcgattctag tcacacatcg ccagacaaat tcttttctgc catgaatatt ccaataggtt
    11101 ctcctataaa tatcgccaca atctccacag aaggtaatgg cacttaaagc gtatttacta
    11161 ctataaattc ttttgttctt gccttctcct gtgtagatat tgcttctccg atgaatctct
    11221 tcctgcacct gcaaaaataa ctctttagga ataatcgcct catggctatt ttcaacataa
    11281 tactggggaa gatggccttc attcttaact cgttttttgg ttagaaaatc tacagtaaca
    11341 gtcttttgta agagggcatc tccgatgtat ttttcgtttt ggaggatttt tttaactgat
    11401 tccggtcgcc atcttgtttt tcccgctgct gttaaaatac catccttttc aagggctcga
    11461 ccaatgccca ctagactctg accctcaagg tattcccggt agatgagttt gataatctca
    11521 gcttcttcgg ggacaatagt taaatttcca tcttcatctt tcgtgtagcc cataaatcgc
    11581 ttatggttga cctgcacctt tccttgttgg tatcggtact gtagcccaag cttaacgttt
    11641 tgtgaaaggc tctggctttc ctgctgtgcc aaagatgcca taatagtcag caaaacctca
    11701 cctttggcat ccgtggtgtt gatgttctct ttctcaaaat aaactgatat gttcttatcc
    11761 ttgagctgtc taatgtattg aaggcagtct agggtgttac gcgcaaatcg gctgatggat
    11821 tttgtaataa ccatgtcgat gttaccgtcc atacactctg caatcatgcg attaaattcg
    11881 tcacgctttt tagtgttcgt accagagata ccatcatctg caaagatgcc agcaaactcc
    11941 cattcattat ttttctttat aaactctgta tagtgtgaga cctgaacctc atagctagaa
    12001 ttttgttctt ctgtttctgt agaaacacgg caataggcag caacacgaag tttctttatc
    12061 ttttcttttg cggctgtact tcctactctt ttacgagcag gaataaccat tatatttttc
    12121 tctgtcactt tattcctcgc tttctatcag actgtataag tattctgctc gttcaaaagg
    12181 atccactggc atcttattat ctgccttttt cattttgaat cgttctttgg gagggggaga
    12241 ggtgaaagcg gcaagctcta ccactcgtcc taaatccttt gcacgtttat ctctaacttc
    12301 ttcagcttta tcaaatatct ctttatcaat aattgctgga tacgtatcat ttccaaggta
    12361 gttgacgttt ttcaaaatgc gccccatcac agaatgtgtc ttttcaatac ctgcctgttc
    12421 gccagccact gtaagggata gtcctgatat gtatttctca aagaatacct ttacttgacc
    12481 tgctgccttt tcatcgacag taaccactcc atcttgaatt ttgtatccat atggaatata
    12541 tgccatttat ctcaccacct tttctttcag ggaaagaccg catttcaaat tgaatgtcag
    12601 ctcatccctg gaatttacaa tgatgttcac tacaaattct tcaaatagtt cttccgtgta
    12661 gtcaccatta aaattatctg ctgacacata attaatgagg tcctttatct cgtttgctcg
    12721 caaaacccca ctcgcagagt tcgttaccag gtttgttttt tcggtagtaa gatttttaat
    12781 ctcactatct aaaacatttc gttcctgatt aaaaagagca ggttctagga aacctttgga
    12841 catcagtgca ataagggtat tgcgttcttc caatagttgc tccattagct tatcaatagc
    12901 gtccattctt tcacggtcgc tttcttcatc gatttggcta actgacttga aaagtggttc
    12961 taggattagc ttatgactga aagcaagctt attcatcatg gttgtcaatg tggctttgat
    13021 ttccccatct cgcaaaaaca acatggagca actttcttta tcttcgatac tatctcaaca
    13081 tgtgtcgtgg ataaggtaag tatgtaaaat ggtatgtgga acatagtgat ttgctgtaaa
    13141 tgatttgcta actactttag ttctttatac aaagtataaa tcggatattt tattattttt
    13201 tcttttatat cttctgagat taggtcaaag tatttgtccg ctaaatcaga aaggggttct
    13261 tttttcatca cctaatgggt gatgaagcag aatactttaa agactattaa atcaatgtta
    13321 ggaaagacta ttagtaaaac tatgaattat tcaggacttt gtttattaat aaaataatta
    13381 ggatcatttc tatttttaaa tatagcactc ttaccgttga caggtactat tccattaatt
    13441 ctctgactaa acattatatc ttgatatggt cttaatccat gtttaagtaa aaatgaaaac
    13501 catgttaaaa cttcaatatt tttaggaatg gctccattta ttttaattat tttctttttt
    13561 atagaatcac aatttgcatt tgtatatgtt gttatcaaaa tctttttatc tttatttttt
    13621 aaagcatcct tcacaattaa tgttgtctta cctgctccag cagcagctat atataattta
    13681 ttattcaaca tcttctatta ccctgtttat atattctgga tattgaattt ttgattctga
    13741 ttcgaatata cttacggcac aatttgtttt atttttctcc atgtatttta atatagagtc
    13801 actatctgtt ttattagtat taaatatact attgagagtt ttcaagtcat ttgctcttaa
    13861 aatacagggc tctaaagtat tgtaattaaa tttatccata tctccactat aagtcttttc
    13921 gtcataatat attttaatct tttcattata ttcataatct ttgtattttc tcattaaagc
    13981 ttctacatct ccgtcattat ctgtaactac atagagattt aagtctaatt tatctcctaa
    14041 ttccaaaaat cttaaaaaag atgttccaac agaaattata tcgataccat taaaaattgg
    14101 taatttgcca tatttatctt tatatgctct ttgtataatt agttcgtctg aaggaccttc
    14161 aactagtata gctttatcac ataataatac tcgtaatgtg tcatatccag aaacagcttt
    14221 aaaatatttt tctgtaccac tacttaaatt tgtaatttta gagcttttct tgtttgataa
    14281 aagtattaaa ttttctaagc ctaatttatt taaaacaaaa ctgctatgag ttgaaataat
    14341 tatttgacta gattccttat tactattttc ttgtagaata ttaattaaat tattcatgtt
    14401 agaatatgac aaatggtttt ctggctcttc aattaaaatt attttatctt tatttgtatt
    14461 aatattattt aaagcaagct cagtttttaa taagcattgt agtcctctac cagcattgga
    14521 atatggaata ctatcaaatt ccgttatcat acttgattcc cagttgaaat tactccctaa
    14581 attaacacct aaactaactt tttttcccaa atttgatttg gatccactta atttttcgtt
    14641 aatattctta acactttcat tcttactaaa atcatcctta gtttctctaa aagcttttga
    14701 tatggaaatt aaatcttcgt tatctaatgt ccctcttatc atattagaaa tatattgatc
    14761 tgaaccatcc ttatatttat aagatgaatt atctattaaa aatgattttg aaggaatgct
    14821 tctagtagta atattttctc tattaaagcc tagccattca cttttataat attctatagg
    14881 taaactgctt aattctcctt tttccataaa tagtttatat tcatcttcat acgaattatc
    14941 aaataatact ttaaatgcaa atcctatttc ttcttttaat cctaagccgt taccactacc
    15001 ctccaaattt gcatcatcta attcagagaa gtatatttct attataattt ctggtaatgc
    15061 acaaggactg ttggaattta tattggttaa atattcatct acaacttctc tattgaataa
    15121 atattgagat aattcacgat tgatataact acctctataa aaaccagtta atgctaagtg
    15181 tatagcttca agtattgttg attttccctc ttcattattt cctaccaaaa tatttatgcc
    15241 accatttaaa tctaattcaa accaatcttt aaaacattta aaatttttaa tcctaacttt
    15301 ttttatcaat tttcaatctc cgtttattat ttcaatcatt tttaatacac tccacaattt
    15361 ctgatatgtc gcaatccaat gcgtcacaaa ttctgagcaa aacatcggta gttacattct
    15421 caccattctt taatttataa aatgtactcc tacttacatt tgctttttcc ataagttcat
    15481 tgtgttgcat atctaaatca ataagttttt taaataacct tttataactt aattccatat
    15541 aacctccaag attatttttt gatagctaat ctcgtacata tatacatttt aaatatactt
    15601 atgtttgtta ttgcatacta attaattata acattaacta taggttattt tcaatcattt
    15661 gatatatcac tttaaatgaa caaaaaaata atggcagtcc gaagactgcc gatatttatc
    15721 tctctgttcc tttgctatgg tctttcttgt ttgacttagc tttgtcatct gtcttaaagc
//
LOCUS       DAUCLR010000035        15507 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.035, whole genome shotgun sequence.
ACCESSION   DAUCLR010000035 DAUCLR010000000
VERSION     DAUCLR010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15507
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.035"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..1590
                     /locus_tag="VGT87_000971"
     CDS             <1..1590
                     /locus_tag="VGT87_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-Rha alpha-1,3-L-rhamnosyltransferase"
                     /protein_id="HEQ3813838.1"
                     /translation="HCLIDEFLQRKNKGFDFSAWHDGLITMGFDKLEEFDSLTIMNDT
                     CFGPIWEMAPYFENFEEKETVDFWGITNNRGTKAFKEHVQSYFMTFKNQVIQNKVFQQ
                     FWQSIIEYENVQEVIQHYETQLTSILLNEGFSYQTVFDTRKAESSFMPHPDFSYYNPT
                     AILKHHVPFIKVKAIDANQHIAPYLLNLIRETTNYPIDLIVSHMSQISLPDAKYLLSQ
                     KYLNCQRLAKQTCQKVAVHLHVFYVDLLDEFLTAFEDWNFHYDLFITTDSDIKRKEIK
                     EILQRKGKTADIRVTGNRGRDIYPMLLLKDKLSQYDYIGHFHTKKSKEADFWAGESWR
                     KELIDMLVKPADSILSAFETDDIGIIIADIPSFFRFNKIVNAWNEHLIAQEMMSLWRK
                     MDVKKQIDFQAMDTFVMSYGTFVWFKYDALKSLFDLELTQNDIPSEPLPQNSILHAIE
                     RLLVYIAWGNSYDFRIVKNPYELTPFIDNKLLNLREDEGAHTYVNFNQMGGIKGALKY
                     IIVGPAKAMKYIFLRLMEKLK"
     gene            1587..4061
                     /locus_tag="VGT87_000972"
     CDS             1587..4061
                     /locus_tag="VGT87_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEQ3813839.1"
                     /translation="MIKDTFLKTNWLNISHHIILLVFGFYFSFYSLAKELVSSTAQPV
                     NYYTHLLNVSFVGYIISLIGLSYYLSRQVSRQLFLKTSFIVISYLIVSYWVQITQHLN
                     DKRFDIWSLTKNQFYQFQALPSLLIILVMATLIKILVAYFAIEKDRFGLLGYQGNTFS
                     VALILAVVPINDIHLLKLISSRFSELVTAGNSQIALLKISGLLIVLLVIFATIIYVVL
                     NALKHLNSNKSSFSVAATTSLFLALVFNYTFQYGVKGDEALLGYYVFPGATLFQIVAI
                     TLVALLAYVITNRYWPTTFFLLILGTIISVVNDLKESMRSEPLLVTDFVWLQELGLVT
                     SFVKKSVIVEMVVGLAICIVVAWYLHGRVLAGKLFMSPVKRASAVLGLFIVSCSMLIP
                     FSYEKEGKILSGLPIISALNNDNDINWLGFSTNARYKSLAYVWTRQVTKKIMEKPTNY
                     SQETIASIAQKYQKLAEDINKDRKNNITDQTVIYLLSESLSDPDRVSNVTVSHDVLPN
                     IKAIKNSTTAGLMQSDSYGGGTANMEFQTLTSLPFYNFSSSVSVLYSEVFPKMAKPHT
                     ISEFYQGKNRIAMHPASANNFNRKTVYSNLGFSKFLALSGSKDKFKNIENVGLLTSDK
                     TVYNNILSLINPSESQFFSVITMQNHIPWSSDYPEEIVAEGKNFTEEENHNLTSYARL
                     LSFTDKETRAFLEKLTQINKPITVVFYGDHLPGLYPDSAFNKHIENKYLTDYFIWSNG
                     TNEKKNHPLINSSDFTAALFEHTDSKVSPYYALLTEVLNKASVDKSPDSPEVKAIQND
                     LKNIQYDVTIGKGYLLKHKTFFKISR"
     gene            4240..4935
                     /locus_tag="VGT87_000973"
     CDS             4240..4935
                     /locus_tag="VGT87_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEQ3813840.1"
                     /translation="MKKLIIIPAYNESSNIVNTIRTIESDAPDFDYIIIDDCSTDNTL
                     AICQKQGFNVISLPINLGIGGAVQTGYRYAQRCGYDVAVQVDGDGQHNPCYLEKMVEV
                     LVQSSVNMVIGSRFITKEGFQSSFARRIGIKYFTWLIALLTGKKITDATSGLRLIDRS
                     LIERFANHYPDDYPEPETVVDVLVSHFKVKEIPVVMNERQGGVSSISLTKSVYYMIKV
                     TLAILVVRLKGNR"
     gene            4937..5278
                     /locus_tag="VGT87_000974"
     CDS             4937..5278
                     /locus_tag="VGT87_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEQ3813841.1"
                     /translation="MTVTASLFISFIVLTFVFFLINLIKKDKLAIKYSLLWFILALLI
                     LLFTWLPNILNKMSHFLGIHSPTNMLFFLGFCLSLAIIFSLTNNISLQNDKVKRLTQE
                     VALMKKEKTND"
     gene            5271..6557
                     /locus_tag="VGT87_000975"
     CDS             5271..6557
                     /locus_tag="VGT87_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEQ3813842.1"
                     /translation="MINPSKQNQTIFLWNMLGSLSTAVISVILLMVVTRLLTSADSDI
                     YAFAYSFANMMVVVGLFQVRNYQATDINEKYSFSQYLVARLMTCLLMLAITVIYLTLT
                     KTDSYKSTIVFLVCFYRSTDAFSDLYQGMFQQHERLDIAGKSLAYRNTLIFMVYTAII
                     LYSKNLTLALVAVCIVSLVFIMYYDIGHSKKFQKLMFSELLSNISFQNSLKLLKESFP
                     LFLNGFLIIYIYTQPKYAIELMTTLGEVALGSQTIFNILFMPAFVMNLLILFFRPHIT
                     QMAIALIRGQIKEFNKIQVQLFAYLGVFSLIALVGSGLFGIPFLSILYGTNLTDYWVD
                     FMLIMLGGSIGSFATVIDNILTAMRKQQLLLIPYTGGFLISLLITNLFVMKYHILGAA
                     LSFLITMLVWLGLSIMIYLFIMNRFKKGRVNATIYD"
     gene            6538..8034
                     /locus_tag="VGT87_000976"
     CDS             6538..8034
                     /locus_tag="VGT87_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HEQ3813843.1"
                     /translation="MQQSMTKVKYLWKENNNYIYSTFLTLLLTVILYANSLNLPLKFI
                     SLLFVVSTLLIVFLPKKLEVATIIFILVFGTISAIISPINDIPDEYVHYSRTVYISEG
                     DINLTNNNKKLRISKDVDKLIKQSGKTFITSNLKATKHSTREYSYPYIKGTNAYYSFS
                     YIPQALGILVGNALDLPILLTYYFGRLCNLISYAMLAFIAIKLSGSFKQVIAVVTLLP
                     MNIYLAASFNQDGFAIGLVLVTIGLFINLLSSKDKSNYNTKFFLYLVLCGLLVLSKFT
                     YFLLVCLPLFIPNEKFGKNTKLVILKKLGGLLLIFLFAAMWFRLYGQVKTPYVADFLK
                     EVNVSQQVKNMLESPIVYSSIIIRHMVINLINMNNIFQFGALSYGITNLFPLYVCFFF
                     FVYISNASKITINIVEKMGIIFVISAIIGATVLAMYLTWTPVGSSTVLGVQSRYLIGI
                     IPLVLLLFSSQQQKFKQIEDILSDKLAIHVSLLFILAMLMSTIFRYYH"
     gene            8128..9351
                     /gene="pepT"
                     /locus_tag="VGT87_000977"
     CDS             8128..9351
                     /gene="pepT"
                     /locus_tag="VGT87_000977"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEQ3813844.1"
                     /translation="MKYDNLLDRFIKYVKVNTRSDPDSETTPSTESQEAFALTILKPE
                     MEAIGLQNVHYNPVNGYLIGTLPANNPTLTRKIGFIAHMDTADFNAENVNPQIIDNYQ
                     GRDITLGSSNYKLDPKAFPNLNNYIGQTLITTDGTTLLGADDKSGIAEIMTAIEFLTS
                     QPQIEHCDIKVAFGPDEEIGVGADKFEVADFEVDFAYTMDGGPLGELQYETFSAAALE
                     VTFLGRNVHPGTAKDQMINALQLAIDFHEKLPAKERPEYTDGYQGFYHLTGLTGTVEE
                     ARASYIIRDFEEASFEARKVKVENIAQSMNAHLGTKRVLVELNDQYYNMKKVIEKDMT
                     AIELAKEVMEELAIKPVIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEFVSLQT
                     MERAVDVIIGLVCKA"
     gene            9392..9880
                     /locus_tag="VGT87_000978"
     CDS             9392..9880
                     /locus_tag="VGT87_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEQ3813845.1"
                     /translation="MIKLFGKIRYHWQPELSWSIIYWSIAFAPIFVGLSLLYERTEIP
                     SRVFILFAIFAVLVGIGLHRYFIIENNGILRIVSFKLFGPRKLLISTITKIEVTKSTL
                     CLHVEDKSYLFYMRKWPKKYFLDALAVNPYFQGEVILSDNFIKLDYFEVYQHDKKALT
                     RG"
     gene            complement(9867..10064)
                     /locus_tag="VGT87_000979"
     CDS             complement(9867..10064)
                     /locus_tag="VGT87_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEQ3813846.1"
                     /translation="MKVSIIPEKCIACGLCQTYSSLFDYHDNGIVTFSSSSETSQSIC
                     PSDKDAILAVKSCPTKALTLE"
     gene            10113..10589
                     /locus_tag="VGT87_000980"
     CDS             10113..10589
                     /locus_tag="VGT87_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEQ3813847.1"
                     /translation="MAKEPWEEKIVDDTIGTRTRKSRNAFISTPWLTALLSVFFVIIV
                     AILFIFFYTSNSGSNRQAETNGFYGASTHKKTKKASNAKKTSSSSTTTDTTPSSEETL
                     ASSEGTGETLTVLAGEGAASIAARAGISVEQLQALNPEHMTQGYWYANPGDQVTIK"
     gene            10604..11284
                     /locus_tag="VGT87_000981"
     CDS             10604..11284
                     /locus_tag="VGT87_000981"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEQ3813848.1"
                     /translation="MKAIKIAIDGPASSGKSTVAKIIAKNLGYTYLDTGAMYRSATYI
                     ALTHGYTGKEVALILEELEKNPIFFKKAKDGSQLVFLGDEDVTLAIRQNDVTNNVSWI
                     SALPEIREELVHQQRRIAQAGGIIMDGRDIGTVVLPDAELKIFLVASVEERAERRYKE
                     NLEKGIESDFETLKEEIAARDYKDSHRKVSPLKAAEDALIFDTTGVSIDGVVQFIQEK
                     AEKIVDMS"
     misc_feature    11308..11431
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            11446..11976
                     /gene="infC"
                     /locus_tag="VGT87_000982"
     CDS             11446..11976
                     /gene="infC"
                     /locus_tag="VGT87_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEQ3813849.1"
                     /translation="MKIIAKKDLFINDEIRVREVRLVGLEGEQLGIKPLSEAQSLADA
                     SNVDLVLIQPQAVPPVAKLMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPVIDKGD
                     FETKLRNGRKFLEKGNKVKVSIRFKGRMITHKEIGAKVLADFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPISDKK"
     gene            12018..12215
                     /gene="rpmI"
                     /locus_tag="VGT87_000983"
     CDS             12018..12215
                     /gene="rpmI"
                     /locus_tag="VGT87_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEQ3813850.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAFTSHRFHGKTKKQRRHL
                     RKAGLVSSGDFKRIKAMVTGL"
     gene            12274..12633
                     /gene="rplT"
                     /locus_tag="VGT87_000984"
     CDS             12274..12633
                     /gene="rplT"
                     /locus_tag="VGT87_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEQ3813851.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVAD
                     AAAFTALADAAKAKLGK"
     gene            complement(12924..15134)
                     /locus_tag="VGT87_000985"
     CDS             complement(12924..15134)
                     /locus_tag="VGT87_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEQ3813852.1"
                     /translation="MTSLGKNDKEVATVKKFKTLITGFINTRLGFIITLLFCYWIKTL
                     WAYHTDFSLDLGNIYQVFLTIINPIPLAFLLFGGALYVKNTRAFYICSWVVYIILNIL
                     LISNSIYYREFSDFITVSAMLASSKVSAGLGDSALNLLRIWDIIYILDFIILISLSIT
                     KKIKNDQRPFNKRAAFAITALSSLLLSINLFLAEIDRPELLTRGFSNTYIVRALGLPA
                     FTLYSGNQTYQAQKERNGATAEELIDVKTYVKGHYAAPDPQYFGIGKGKNIIVLHLES
                     FQQFLIDYKLKEGDKEYEVTPFINSLYHSNATLAFPNFFHQVKAGKTSDAETMMENSL
                     FGLNSGSFMVNYGGENTQFATPSILTQKGGYTSAVFHGNVGTFWNRNNAYKQWGYNYF
                     FDSSYFSKQNSKNSFQYGLNDKYMFKDSIKYLEQMQQPFYTKFITVSNHYPYTSLKGE
                     SSEEGFPLAKTDDETINGYFATANYLDAALKSFFDYLKATGLYDNSIFVLYGDHYGIS
                     NSRNSSLAPLLGKDSETWSEYDNAMLQRVPYMIHIPGYTNGSIKETFGGEIDALPTLL
                     HILGIDTSQFVQLGQDLLSPQNSQIVAQRTSGTYMTPEYTNYSGRLYNTQTGLEITNP
                     DEMTIAKTKEIRSAVAQQLAASDAIQTGDLLRFDTQNGLKAIDPNQFIYTKQLKQLKD
                     ISAKLGSESTSLYSKNGHKSTQKLFKAPSYLELNPVEADAATSELKEDNTKNRE"
     gene            15242..>15507
                     /locus_tag="VGT87_000986"
     CDS             15242..>15507
                     /locus_tag="VGT87_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEQ3813853.1"
                     /translation="MNKLYIDSFVEKKLTAGVQLLDEKDFSNIKEKNQLVQLVTKSNR
                     PIGTAYISKQNKGIGWYLGPEKIDLSISYFVSLFSVAKAKRQDFA"
BASE COUNT         5097 a         2384 c         2834 g         5192 t
ORIGIN      
        1 cactgtctta ttgatgagtt tttgcaacgc aaaaataaag gttttgattt ttctgcatgg
       61 cacgatggct tgattactat gggatttgac aaattggaag agtttgactc attgacgatt
      121 atgaacgata cttgttttgg tcctatttgg gagatggctc cttattttga aaattttgaa
      181 gaaaaagaga cagttgactt ttggggaatt actaataata gaggtacaaa agcttttaaa
      241 gaacatgtcc aaagttattt tatgacgttt aaaaatcaag tcattcaaaa caaagttttt
      301 caacaatttt ggcaatcgat aatagaatat gaaaacgttc aagaagtgat tcaacattat
      361 gaaacgcaat taacatccat tctcttaaat gaaggcttta gttatcagac ggtctttgat
      421 actagaaaag ctgagtcgtc attcatgcca catccggatt tttcatatta taatccaaca
      481 gcaattttaa aacaccatgt gccttttata aaagtaaaag ctattgatgc taaccagcat
      541 atcgctcctt acttactgaa tctgatacga gaaacaacca attatcctat agatcttatt
      601 gtttcacata tgtcgcaaat ctctttgcca gatgctaagt accttttatc tcaaaaatac
      661 ctaaactgcc aacggttagc aaaacaaact tgtcaaaagg tagctgttca tttacatgtt
      721 ttttacgttg atttgctaga tgaattttta acagctttcg aagactggaa cttccattat
      781 gatcttttta ttacaacaga tagtgatatc aaaagaaaag agatcaaaga aatacttcaa
      841 agaaaaggta aaactgctga tatacgcgtt acgggaaata gaggaagaga tatttatcca
      901 atgttattac taaaagataa actttctcaa tatgattata ttggtcattt ccatacgaaa
      961 aaatcaaaag aagctgattt ttgggcagga gaatcttggc gaaaagaatt gattgatatg
     1021 ctcgtgaaac cagcagatag tattcttagc gcttttgaga cagatgatat tggtattata
     1081 atcgcagata ttccaagttt ttttagattt aataagattg tcaatgcttg gaatgagcat
     1141 ttaatcgctc aagaaatgat gtctttgtgg agaaaaatgg atgttaaaaa acaaattgat
     1201 tttcaagcta tggatacatt tgttatgagt tatggaacat ttgtatggtt taaatacgat
     1261 gctctaaaat cactttttga tttagaatta actcaaaatg atattccaag tgaaccttta
     1321 ccacaaaact ctatattaca tgcgattgag cggttacttg tctatatagc gtgggggaat
     1381 tcttatgatt ttaggattgt taaaaatccc tatgagttaa caccttttat tgacaataaa
     1441 ctgttaaatt tacgtgaaga tgaaggagct catacctacg ttaactttaa tcaaatgggc
     1501 ggtatcaaag gagcattaaa atatattatt gttggtcccg ctaaagcaat gaagtatata
     1561 ttcttgcgtt taatggagaa acttaaatga ttaaagacac atttttaaaa accaattggt
     1621 taaatattag tcaccatatt atccttcttg ttttcggttt ttatttcagt ttttacagtt
     1681 tggcgaaaga actagtaagc tccacggcac aaccggtaaa ctattatact catttactaa
     1741 atgtttcttt tgtgggatat attatatcac tgattggatt atcttattat ttgagtcgcc
     1801 aagttagtcg acagttgttt ttgaaaacta gttttattgt gatatcttat ctaattgtca
     1861 gctattgggt acaaataaca cagcacctga atgataaacg gtttgatatc tggtcattaa
     1921 ctaaaaatca attttatcaa tttcaagctc taccttcttt actcattatt ttagtgatgg
     1981 ccactttaat aaaaatattg gtagcatatt ttgcaataga aaaagataga tttgggctat
     2041 taggctatca aggtaatact ttttctgtag ctctgatttt agcagttgtg ccaattaatg
     2101 acatacatct gttaaaacta ataagttctc gattttctga attagtaaca gcaggtaata
     2161 gccaaattgc actgttaaaa ataagtggac tgttgatagt tttacttgtc atatttgcaa
     2221 caatcatata cgtggtttta aatgctctaa aacaccttaa ctcaaataaa tcttcatttt
     2281 cagtagcagc tactactagt ttgtttttag cattagtttt taactatacg ttccagtatg
     2341 gcgtaaaagg tgatgaagca ttgctaggat attatgtttt ccctggagct actctttttc
     2401 agatagtagc tattacacta gttgctcttt tagcatacgt gataacgaat agatattggc
     2461 caactacctt ctttttgctt attctgggaa caattatttc tgttgttaat gatttaaaag
     2521 aatcaatgag aagcgagccg ttattagtaa ctgattttgt ttggttacaa gaattaggtt
     2581 tagtgacaag ctttgttaaa aaatcggtga ttgtagaaat ggttgtagga cttgctattt
     2641 gtattgtggt ggcttggtat ctacatggcc gagttttagc agggaaatta tttatgagcc
     2701 ctgtcaaacg ggcaagtgct gtattaggtt tatttattgt atcttgtagt atgttaatac
     2761 cattttctta tgaaaaagaa ggtaaaatat tatctggtct tccgattatt tcggctttaa
     2821 ataatgataa tgatataaac tggttaggtt tttcaacaaa tgctaggtac aaatctttag
     2881 catatgtttg gacaagacag gtgaccaaga aaataatgga aaaaccgaca aattatagtc
     2941 aagaaacaat agcgagtatc gctcagaagt accaaaaatt agcagaagat attaataaag
     3001 acagaaaaaa taatattact gaccaaacgg ttatttatct tttaagtgaa agcttgtcag
     3061 atcctgatag agtatcaaat gttactgtta gccacgatgt tttacctaat atcaaggcaa
     3121 tcaaaaatag cacaactgcg ggactcatgc agtcagactc ctacgggggt ggaacggcta
     3181 acatggagtt tcaaacgtta acaagcttac ctttttataa tttttcttct tcagtatctg
     3241 ttctttattc agaagtcttt cctaaaatgg ccaaacctca tacgattagt gagttttacc
     3301 aaggaaaaaa tcgtattgcg atgcatcctg ctagtgctaa caattttaat agaaaaacag
     3361 tttatagtaa tttaggtttt tccaaattct tagctctatc gggttctaag gataagttta
     3421 agaacattga aaatgtcggt ttattgacta gcgataaaac tgtctataat aatattttat
     3481 ctttaattaa tcctagtgaa agccaatttt tttcagttat tacaatgcaa aatcatattc
     3541 cttggtcatc cgattatcct gaagaaattg ttgctgaagg aaaaaatttc acggaagaag
     3601 aaaatcacaa cctaacaagt tatgctcggt tattatcgtt tactgataag gaaacaagag
     3661 catttttaga aaaattaaca caaattaaca agcctatcac agtggtgttt tacggagatc
     3721 atttacccgg tttatatcct gatagtgctt ttaacaagca tattgaaaat aaatacctta
     3781 ctgattattt tatttggagt aatggtacta acgagaaaaa aaatcatccg cttatcaact
     3841 caagtgattt tactgcagct ttatttgagc atactgattc aaaagtatca ccttactatg
     3901 ctttgttaac agaggtactg aataaagcta gtgtcgataa atcaccagat agtcctgaag
     3961 ttaaagctat tcagaatgat ttaaaaaata tccaatacga tgtgactata ggaaaaggtt
     4021 accttttgaa acacaaaact ttttttaaga tatcacgtta agaaaaatgc taggctgtcc
     4081 gagtataggc aagcatcaag gtttggtaat ttgctcaaat gattgtcaac agagtgtcta
     4141 ggaccaggtt taaaagatta taattcttaa ggtcttattc gtttataaaa aatgaatact
     4201 cttttaaaat cttaattgaa atgaatagga gaagttttcg tgaaaaagtt aatcattatt
     4261 cctgcttaca atgaaagcag taatattgtc aatactatac gtactattga atcagatgcc
     4321 ccggattttg actatatcat tattgatgat tgctcaacgg ataatacgtt agcaatatgt
     4381 caaaaacagg ggttcaatgt tatttctttg cccattaacc tgggaattgg cggtgcggtg
     4441 caaactggct atcgttatgc acaaagatgt ggatatgacg ttgcagttca agtagatgga
     4501 gatggtcagc acaatccatg ctatttggaa aaaatggttg aggtattagt tcaatcttca
     4561 gtaaatatgg taattggatc acgatttatc acaaaagaag ggtttcagtc atcatttgct
     4621 cgacgtatcg gcataaagta ttttacttgg cttattgcgc tactaacagg aaaaaaaata
     4681 acagatgcaa cgtcaggtct aaggttaatt gaccgttcat tgattgaacg ttttgctaat
     4741 cattatccag atgattatcc tgaacctgaa acagttgttg atgtattggt tagtcatttt
     4801 aaagtgaaag aaattcctgt tgtaatgaat gagcgacaag gcggtgtgtc atctatctcg
     4861 ttgacgaaat cggtctatta tatgattaaa gtgactttag ctattttagt tgttagattg
     4921 aaaggaaatc gttaaaatga cggtaacagc cagtttattt ataagtttta ttgtattaac
     4981 ctttgttttc tttttgatta acttaatcaa aaaagataaa ctagcgatta agtactcttt
     5041 attgtggttt attttagctt tattaatttt actatttacg tggttaccta acatattaaa
     5101 taaaatgtct cacttcctgg gtattcattc accaaccaat atgttattct ttttaggttt
     5161 ttgtttgagt ttagctatta ttttttcact gactaataat atttcacttc aaaatgataa
     5221 agtaaaacgt ctgacccaag aagtggccct tatgaaaaaa gagaaaacca atgattaatc
     5281 cttcaaaaca aaaccaaacg atttttttat ggaatatgtt aggaagttta tcaacagctg
     5341 taatatcagt tatattactt atggtggtta ctaggttact aacttcggct gattcagata
     5401 tttacgcctt tgcctattct tttgctaata tgatggtagt tgttggactt tttcaggttc
     5461 gtaattatca agcaacagat attaatgaaa aatattcctt tagtcagtac ttagtggcaa
     5521 gactgatgac ttgtttatta atgttagcca taacagtaat ttatttaacg ttaactaaga
     5581 cagatagcta caagagtaca attgtctttc tagtctgttt ctatagatcg acagatgctt
     5641 tttcggattt atatcaggga atgttccaac aacatgaacg gctggatatt gcaggtaaat
     5701 cgctagccta cagaaatact cttattttta tggtgtacac agcaataata ttatattcca
     5761 aaaatcttac tttagcatta gtagctgtct gtatagtttc attagttttt ataatgtatt
     5821 atgacattgg tcattcgaaa aaatttcaaa agttgatgtt tagtgaatta ttaagtaata
     5881 tttcatttca aaacagttta aagttattaa aagaaagttt tccacttttt ctaaacggtt
     5941 ttttgattat ctatatttat actcaaccaa aatacgctat tgaactaatg acgactttag
     6001 gtgaagttgc tttgggctct caaacaattt ttaatatttt atttatgcca gcctttgtta
     6061 tgaatttgct aattttattt tttagacctc atattacaca gatggctatt gctttaatca
     6121 gaggtcaaat aaaagagttt aataagatac aagttcaatt gtttgcttat ttaggagttt
     6181 tttcactgat agcgttagtt ggaagtggtt tgtttggtat tcccttttta tcaatactat
     6241 atggtactaa ccttacggat tattgggtag attttatgtt gattatgcta ggaggctcga
     6301 ttggtagttt tgcgacagtt atcgataata tcttaaccgc tatgagaaaa cagcaacttc
     6361 ttcttattcc atatacagga ggatttttga tttcgttatt aattaccaat ctttttgtta
     6421 tgaaatatca tattttagga gctgctttga gctttttaat aacaatgttg gtttggttgg
     6481 gattatccat tatgatttat ctatttatta tgaatagatt taagaaggga agagttaatg
     6541 caacaatcta tgactaaagt taaatatctt tggaaagaaa ataataacta tatttattcg
     6601 acttttttga cattacttct gacagttatt ttatatgcca atagtttaaa tctaccttta
     6661 aaatttatta gtcttctttt tgtggtgagc actttactga tagtgttttt accaaaaaag
     6721 ttagaggttg ctacgatcat ttttatttta gtatttggaa cgatatctgc tatcatatca
     6781 cctataaatg atattcctga tgaatatgtt cattattcaa ggacagttta tatctcagaa
     6841 ggtgatatta atcttaccaa caataacaaa aagttaagaa tatccaaaga tgttgataag
     6901 cttattaaac agtcaggaaa aacatttata acatcaaact tgaaagcaac taaacacagc
     6961 acaagagagt atagctatcc ttacatcaaa ggaactaatg catattatag tttttcttat
     7021 ataccacaag ctttgggtat tttagtagga aatgcgctgg atttacctat acttttaaca
     7081 tattatttcg gaagattgtg caaccttatt agctacgcta tgcttgcttt tatagctatc
     7141 aagttgtctg gttcttttaa acaagtgata gctgttgtta ctttactacc tatgaacatt
     7201 tatttagcag catcttttaa tcaagacggc tttgctatcg gtttagtttt agtaacaatt
     7261 ggattgttta ttaacttgct ctcatcaaaa gataaatcta actataatac caaattcttc
     7321 ctttatttag ttctatgtgg gttactagtt ctttcaaaat tcacatactt tttattagta
     7381 tgccttccat tatttattcc taatgaaaaa ttcggaaaaa ataccaagtt agtcatcttg
     7441 aaaaaattgg gaggactatt actcattttt ctttttgctg caatgtggtt tagactttat
     7501 ggacaggtta aaaccccata tgtcgcggat tttctaaaag aagtaaacgt tagccagcaa
     7561 gttaaaaata tgctagaatc ccctatagtc tatagcagta ttattattag acatatggta
     7621 attaatctga ttaatatgaa taatattttt cagtttggcg cattaagcta tggtataacg
     7681 aaccttttcc cgctttatgt gtgtttcttc ttttttgttt atattagtaa tgctagtaaa
     7741 ataactatta atattgtaga aaaaatgggt ataatctttg ttatttcagc gattataggt
     7801 gcaactgtat tagctatgta cctgacttgg acaccagtag ggtcctcaac tgtcttaggt
     7861 gttcaaagtc gttatctgat aggaattatc ccattagtgt tgcttctttt tagtagtcaa
     7921 cagcaaaagt tcaaacaaat agaggatatc ctttctgaca aactggctat tcatgtttcg
     7981 cttttgttta ttctcgcaat gttaatgagt actattttta gatattatca ttaaactctt
     8041 aattagaggc tttaaaaaac ttagcttgaa taagtatttt aaagcctttt atgctataat
     8101 taaattgaga aattattgag gtggcctatg aagtacgata acttgttaga tcgatttata
     8161 aaatatgtca aagtgaatac aagaagcgat ccagacagtg agactactcc tagtacggag
     8221 agtcaagaag catttgcctt aacgatttta aagcctgaaa tggaggcaat tggtttgcaa
     8281 aatgttcatt ataatccagt taatggctat cttattggaa ctctgcctgc caataatcca
     8341 accttgacgc gtaaaattgg ttttatagcc catatggata cggctgattt caatgctgaa
     8401 aatgttaatc cgcagattat tgacaattat caaggaaggg acattacact tggcagttct
     8461 aattataagt tggatccaaa agcatttcca aatcttaata attatattgg tcaaacattg
     8521 attaccactg atgggactac acttttaggg gcagatgata aatctggtat tgctgaaatc
     8581 atgacagcaa tagaattctt gacatcacaa ccacaaatag agcactgtga cattaaagtg
     8641 gcttttggcc ctgatgaaga gattggggtc ggagctgata agtttgaagt ggctgatttt
     8701 gaggtggatt ttgcttatac tatggatggc ggtcctttag gcgaattgca atatgaaact
     8761 ttcagcgctg cagctctaga agtgactttt ttaggcagaa atgttcatcc tggtacggct
     8821 aaggatcaaa tgattaatgc ccttcagtta gcaatagatt ttcatgagaa gctacctgct
     8881 aaagagcggc ctgaatacac agatggttat caaggcttct atcacttaac cggactaact
     8941 ggaacagttg aagaggcacg cgcatcttac attattcgtg attttgaaga agcatcattt
     9001 gaagctagaa aagtgaaagt tgaaaatata gctcaatcga tgaatgctca tctaggaaca
     9061 aaacgtgtct tggtagagct taatgaccaa tactacaata tgaaaaaagt tatcgaaaaa
     9121 gatatgaccg ctattgagtt agctaaagaa gtaatggaag agcttgctat caagcctgta
     9181 atcgaaccca tacgtggagg aacagatggc tctaaaattt catttatggg tattcccact
     9241 cctaatattt ttgcaggggg tgagaatatg cacgggcgtt ttgagtttgt tagcttacaa
     9301 acgatggaga gagctgtcga tgtcattatt ggacttgttt gcaaagccta attatcatgt
     9361 aattgatatt tagtggaagg ggcttataaa aatgattaaa ctatttggta aaataaggta
     9421 tcactggcag ccagagttgt cttggtctat tatttattgg tctattgcgt ttgcacctat
     9481 ttttgtaggt ttatctttgt tgtatgagcg cactgaaata cctagtcggg tttttattct
     9541 atttgctatt tttgctgtcc tagttggcat tggattacat cgttatttca ttattgaaaa
     9601 taatggtatt ttaagaatcg tatcttttaa attgtttggt ccacgtaagc tacttatttc
     9661 tactattact aagattgagg tgaccaaatc aacactttgt ctgcacgttg aagataaaag
     9721 ttatctcttt tatatgcgca aatggcctaa aaaatatttt cttgatgcct tagcggttaa
     9781 tccatacttc caaggagaag ttatattaag tgataacttt attaaattag actattttga
     9841 agtttatcag catgataaaa aagcccttac tcgagggtaa gagctttagt tgggcaagat
     9901 ttgacagcta aaatagcgtc cttatctgaa ggacaaattg actgacttgt ttcagatgag
     9961 cttgaaaatg tgacgatacc attgtcatga taatcaaaaa gtgatgaata ggtttggcat
    10021 agaccacaag cgatgcattt ttctggaatg atagatactt tcataactat tattctaata
    10081 tgattttgtt aaattaacaa ggggggacga tcatggctaa agaaccatgg gaagaaaaaa
    10141 ttgttgatga tactataggg acacgaacac gtaaatcaag aaatgctttc attagcacgc
    10201 cttggttgac tgctttatta agtgtattct ttgtcatcat tgttgctata ctttttattt
    10261 tcttctatac atcaaatagc ggtagtaata gacaagctga aacaaatggt ttttatggag
    10321 catccactca taaaaaaaca aagaaagctt ctaacgctaa aaaaacatca agtagttcaa
    10381 caactacaga cacaacacct tctagcgaag aaacacttgc ttctagtgaa ggaactggcg
    10441 aaacccttac tgtattggca ggtgaggggg cagcttctat tgcagcccgt gcaggtattt
    10501 ctgtggagca gctacaagca cttaatccag agcacatgac tcaaggatat tggtatgcca
    10561 atccaggaga tcaagtcact attaaataat tgaagaggaa aaaatgaaag caattaaaat
    10621 tgcgattgat gggccagctt caagtggaaa aagtacagta gccaagatta ttgcaaaaaa
    10681 tcttggctat acttatctag atactggtgc tatgtatcgc tcagcaactt atatcgcact
    10741 gacacatggc tatacgggta aagaagtagc ccttatttta gaggaacttg agaaaaatcc
    10801 tattttcttt aaaaaagcaa aagatggctc ccaattagtt tttcttggag atgaggacgt
    10861 gaccttagcc attaggcaaa atgatgtgac taacaatgtt tcttggatct cagctttgcc
    10921 agaaattaga gaagagctgg ttcatcaaca aagacgcatt gcacaagctg gtggcattat
    10981 catggatggt cgtgacattg gaacggttgt tctcccagat gcggaactaa aaatcttttt
    11041 agtggcttct gtcgaagagc gggcagagcg ccgctataaa gaaaacctag aaaaaggaat
    11101 tgaatcagat tttgagacgc taaaagaaga gattgcagct cgtgattaca aggatagtca
    11161 tcgtaaggtt tcacctctaa aagcagctga agatgccctt atttttgata ccacaggtgt
    11221 ttctattgat ggtgtggtgc aatttatcca agaaaaagca gaaaaaatag ttgacatgtc
    11281 ctagagccta tgttagaata ttaacaatga gaaaagtaga agtgagagct tctcgccttg
    11341 cgacttaggt tgtctggccc taacatgtcg aattcctatg gaatattatg tgtgcgggct
    11401 tgatttatca agtccgcttt cgtttttctc taaaaaaata aagaggtgaa gatcatagct
    11461 aaaaaggatc tattcattaa tgatgaaatt cgcgttcgcg aagttcgtct cgttggtctt
    11521 gaaggtgaac aattaggtat taaaccattg tctgaagcgc aatcattggc tgatgcttca
    11581 aatgttgatt tggttttaat ccagccacaa gctgttcctc ctgttgccaa acttatggac
    11641 tatggaaagt tcaaatttga gtatcaaaag aaacagaaag aacaacgtaa aaaacaaagc
    11701 gttgtgactg tgaaagaagt tcgtctcagt ccagttatcg ataaaggtga ctttgaaaca
    11761 aaacttcgta acggtcgtaa attccttgaa aaaggaaata aagttaaggt ttctattcgt
    11821 ttcaaaggac gtatgattac tcataaagag attggtgcca aggtactagc tgactttgct
    11881 gaagcgactc aagatattgc tatcattgag caacgagcaa aaatggatgg acgccaaatg
    11941 tttatgcaac ttgcaccaat ttcagacaag aagtaattgt cacagtttca ttatatcttt
    12001 aagaggagaa tactaaaatg ccaaaacaaa aaacacaccg cgcatcagct aaacgtttta
    12061 aacgtacagg ttcaggcggt ttgaaacgct tccgcgcctt tacatcacac cgtttccacg
    12121 gaaaaactaa aaaacaacgt cgtcatcttc gtaaagctgg tttggttagc tcaggagatt
    12181 tcaaacgtat caaagcaatg gttactggtc tttaatttta aaggcagtag acatttacga
    12241 gtattataag aattatttga aggagaacta ttaatggcac gtgttaaagg tggcgttgtt
    12301 tcacgcaaac gtcgtaaacg tattttaaaa ttagctaaag gttactatgg agcaaaacac
    12361 atcttgttcc gtactgcaaa agagcaagtg atgaactctt actactatgc ataccgtgac
    12421 cgccgtcaga aaaaacgtga tttccgtaaa ttatggatca ctcgtatcaa tgcggctgct
    12481 cgtatgaacg gtttgtcata ctcacaattg atgcacggtt tgaaacttgc tgaaatcgaa
    12541 gtaaaccgta aaatgcttgc tgatttagca gtagctgatg cagcagcttt cacagctctt
    12601 gcagatgcag ccaaagcaaa acttggcaaa taagaaacgc tatacctaga aaataacttt
    12661 ttattaccta tagaaagtta taaagaaaca aaaatgaagg agacgatgga cgtcttcttt
    12721 ttattatact caatataata aaaagaagtt tcccatgttt tattaccagt aatgtgggat
    12781 atttagatgg tagcaagaag ttttatagtt gatttgtttt ctttaggtct aattagcata
    12841 ttttgattac tgataaactt gaatatcgcc aataaaaaag aagcaaaatt attattttgc
    12901 ttctagtcag atcattaaat tgattattct ctatttttgg tgttatcctc ttttagttca
    12961 gaagttgccg cgtcagcttc tacgggattt agttctaagt aagatggtgc tttaaaaagt
    13021 ttctgagttg atttatgacc atttttactg tataaacttg ttgactctga tccgagtttt
    13081 gctgaaatat ctttcagttg ttttaactgc ttagtgtaga taaactggtt aggatcaata
    13141 gcttttagac cattttgagt atcaaagcgc aggagatcac ccgtttgaat agcgtcgcta
    13201 gctgctagtt gttgagcaac agcagagcga atttccttag ttttggctat agtcatttca
    13261 tcgggattag tgatttctaa acctgtttgc gtgttgtaca gacgtccact atagttagtg
    13321 tattcaggag tcatataagt cccagatgtt cgttgtgcca caatctggct attttgaggt
    13381 gataataaat cttgtcctaa ttgaacaaac tgactagtgt caataccaag tatgtggagt
    13441 aaagtaggaa gagcatcgat ttcaccgcca aaggtttctt tgatactacc attcgtataa
    13501 cctggaatat ggatcatata cgggactcgt tgtagcatag cattatcata ttcagaccaa
    13561 gtttcagaat ctttaccaag aagtggagca agactagaat tacgagaatt tgaaatacca
    13621 taatggtcac catataaaac aaaaatagaa ttgtcgtaca aaccagtggc tttcaagtaa
    13681 tcaaaaaaag atttaagggc agcgtctaaa taattggcag tagcaaagta accattgatc
    13741 gtttcatcgt ctgttttggc taaaggaaaa ccttcttcac tactttcgcc ttttagacta
    13801 gtataaggat agtgattact cacagtgata aacttggtat aaaaaggttg ttgcatctgt
    13861 tcaagatatt ttatggaatc cttaaacatg tacttatcat ttaacccata ttgaaaagag
    13921 ttcttactat tttgtttaga gaagtagcta gaatcaaaaa aataattata gccccattgt
    13981 ttataagcat tattgcgatt ccagaaagtt ccaacgttac catgaaagac agcgctggta
    14041 tagccacctt tttgggttaa aatacttgga gtagcaaatt gtgtattttc accaccgtag
    14101 ttcaccataa aagaaccact atttaaacca aataaggaat tctccatcat ggtttcagca
    14161 tcagaagttt tacctgcttt aacctgatga aaaaagttag ggaatgctaa ggtagcattt
    14221 gagtgataaa gcgagttgat aaaaggcgtt acctcatatt ctttatctcc ttcttttagt
    14281 ttataatcta ttaagaattg ttgaaagctt tctaaatgaa gaacaataat atttttgcct
    14341 tttccaattc caaaatactg aggatcaggt gctgcataat gtcctttgac ataagtttta
    14401 acatcaatta attcttctgc agtagctcca tttcgttctt tttgagcttg ataggtttga
    14461 ttgccgctat agagggtaaa cgctggtagt cctaaagctc tcacaatata agtatttgaa
    14521 aagccacgtg ttaatagttc cggacgatca atttctgcta aaaaaagatt aatagaaagc
    14581 aatagactag ataaagcggt gatggcaaat gctgcgcgct tgttaaaagg acgttggtca
    14641 tttttgattt ttttagtgat agataaacta attagaataa taaaatcaag aatatagata
    14701 atatcccaga tgcgtaaaag gtttaaagca gagtccccta atccagcgga gactttacta
    14761 ctagctagca tggcactcac tgtaataaag tctgaaaatt ctcggtaata tatagaattt
    14821 gatatcaaga gaatgttaag gataatataa accacccaag aacagatata aaaggctcgg
    14881 gtattcttaa cgtaaagagc acccccaaaa agtaaaaaag ctagtgggat tggattgata
    14941 atagttagaa agacttggta tatatttccc aaatctagtg aaaagtctgt atgatatgcc
    15001 cacagggtct ttatccagta acaaaagagt agagtaatta taaaacccaa tcttgtgttg
    15061 ataaaacctg tgataagtgt tttaaatttc ttcacagtag caacttcctt atcgtttttt
    15121 cctaaagatg tcatctccaa ataaaatctc agattaaatt ataccaaaaa tgtgaagcta
    15181 atgcttgttg taagttcaaa tttagtagga ttttttatca gattttgtta taataaaaac
    15241 tatgaataaa ctctatattg attcttttgt cgaaaagaag ctgacagcag gggtacaatt
    15301 attagatgaa aaagattttt ctaatataaa ggaaaaaaat cagttggttc aactggtgac
    15361 caagtccaat cgtcccattg gaacagcata tatctctaag caaaataaag gaattggttg
    15421 gtatctagga ccagaaaaaa tcgacttgtc tatctcttat tttgttagtc tattttcagt
    15481 tgctaaggcc aaaagacaag attttgc
//
LOCUS       DAUCLR010000036        15499 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.036, whole genome shotgun sequence.
ACCESSION   DAUCLR010000036 DAUCLR010000000
VERSION     DAUCLR010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15499
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.036"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..796
                     /locus_tag="VGT87_000987"
     CDS             <1..796
                     /locus_tag="VGT87_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEQ3813854.1"
                     /translation="FMITPYSKKKEFSVRGNHQTNLDELIKASKVKASDYWLTLLTSP
                     GQYERPILRTIPWVKSVHLSYQFPNHFLFNVIEFEIIAYAQVENGFQPILENGKRVDK
                     VRASELPKSFLILNLKDEKAIQQLVKQLTTLPKKLVKNIKSVSLANSKTTADLLLIEM
                     HDGNVVRVPQSQLTLKLPYYQKLKKNLENDSIVDMEVGIYTTTQEIENQPEVPLTPEQ
                     NAADKEGDKPGEHQEQTDNDSETPANQSSPQQTPPSPETVLEQAHG"
     gene            1008..2372
                     /gene="ftsA"
                     /locus_tag="VGT87_000988"
     CDS             1008..2372
                     /gene="ftsA"
                     /locus_tag="VGT87_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEQ3813855.1"
                     /translation="MARNGFFTGLDIGTSSIKVLVAEFISGEMNVIGVSNVPSTGVKD
                     GIIIDIEAAATAIKTAVEQAEEKAGMTIEKVNVGLPANLLQIEPTQGMIPVPSESKEI
                     KDEDVDSVVKSALTKSITPEREVISLVPEEFIVDGFQGIRDPRGMMGIRLEMRGLIYT
                     GPSTILHNLRKTVERAGIKVENIIISPLAMAKTILNEGEREFGATVIDMGGGQTTVAS
                     MRAQELQYTNIYAEGGEYITKDISKVLKTSLAIAEALKFNFGQAEISEASITETVKVD
                     VVGSEEPVEVTERYLSEIISARIRHILDRVKQDLERGRLLDLPGGIVLIGGGAIMPGV
                     VEIAQEIFGVTVKLHVPNQVGIRNPMFSNVISLVEYVGMMSEVDVLAQTAVSGEELLR
                     RKPIDFSGQESYLPDYDDSRRPESTIGYEQQASQTAYDSQVPSDPKQKISERVRGIFG
                     SMFD"
     gene            2396..3715
                     /gene="ftsZ"
                     /locus_tag="VGT87_000989"
     CDS             2396..3715
                     /gene="ftsZ"
                     /locus_tag="VGT87_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEQ3813856.1"
                     /translation="MAFSFDTASIQGAIIKVIGVGGGGGNAINRMIDEGVAGVEFIAA
                     NTDIQALSSSKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEEILTEALTGADMVFI
                     TAGMGGGSGTGAAPVIARIAKSLGALTVAVVTRPFGFEGNKRGNFAIEGIEELREQVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITSPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERIVEAARKAIYSPLLETTIDGAQDVIVNVTGGLDMTLTEA
                     EEASEIVGQAAGQGVNIWLGTSIDDTMKDDIRVTVVATGVRQEKAEQVSGFRQPRTFT
                     QTSAQQVAGAQYASDQAKQSVQPGFDRRSNFDFDMGESREIPSAQKVISNHNQNQGSA
                     FGNWDLRRDNISRPTEGELDNHLNMSTFSANDDSDDELETPPFFKNR"
     gene            3718..4389
                     /locus_tag="VGT87_000990"
     CDS             3718..4389
                     /locus_tag="VGT87_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEQ3813857.1"
                     /translation="MDLLTNKKKIFETIRLSTEAANRTNDSVSVIAVTKYVDSTIAGQ
                     LIEAGIEHIAENRVDKFLEKYDALKYMPVKWHLIGTLQRRKVKEVINYVDYFHALDSV
                     RLALEINKRADHPVKCFLQVNISKEESKHGFNISEIDEAIEEIGKMEKIQLVGLMTMA
                     PANASKGSIITIFRQANQLRKNLQLKKRKNMPFTELSMGMSNDYPIAIQEGSTFIRIG
                     RAFFH"
     gene            4401..5057
                     /locus_tag="VGT87_000991"
     CDS             4401..5057
                     /locus_tag="VGT87_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEQ3813858.1"
                     /translation="MAFKDTFNKMISYFDTDEVNEVEEDVAASTDNVIPRSQQSVRAS
                     SHPKQEPRNNHVQQDHQARSQEQTRSQMHPKHGTSERYYQQSQPKEGHEMVDRRKRMN
                     TSGIANRREQYQQSTCSDQTTIALKYPRKYEDAQEIVDLLIVNECVLIDFQFMLDAQA
                     RRCLDFIDGASKVLYGSLQKVGSSMYLLAPSNVSVNIEEMTIPHTTQDIGFDFDMKRR
                     "
     gene            5062..5316
                     /locus_tag="VGT87_000992"
     CDS             5062..5316
                     /locus_tag="VGT87_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEQ3813859.1"
                     /translation="MILILSILLRLIKVYTYLLIAYALMSWFPGAYDSKIGRLISGIV
                     EPILKPFRAFNLQFAGLDFTIFVVIISLNFLAQVLVRVFI"
     gene            5316..6107
                     /locus_tag="VGT87_000993"
     CDS             5316..6107
                     /locus_tag="VGT87_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEQ3813860.1"
                     /translation="MVSHSKIYQHFHQEEYPFIDRMSDMINRVEDYYLLEVTEFLNPR
                     EVMILKSLIALTDLKMFVSTDYYPSEYGRVIIAPGYYDLEQSDFQIALVEISYQAKFN
                     QLTHSQILGTLINELGVKRNLFGDVFVEMGYAQLMIKRELLDYFLGTITKIAKTSVKL
                     REVNFDQLIRSIDNSQTLDILVSSFRLDGVVATILKKSRTQVIALIEANKIKVNYRLA
                     NKASDNLVIGDMVSIRGHGRFTLLADNGVTKHGKQKITLSKMIHK"
     gene            6117..6875
                     /locus_tag="VGT87_000994"
     CDS             6117..6875
                     /locus_tag="VGT87_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEQ3813861.1"
                     /translation="MALTTLEIKDKTFKTKFRGYCEEEVNEFLDIVVDDYEALVRKNR
                     DNEARIKDLEEKLSYFDEMKESLSQSVILAQETAEKVKATANAEATNLVSKATYDAQH
                     LLDESKAKANQMLRDATDEAKRVAIETEELKRQTRVFHQRLISSIESQLSLSNSPEWD
                     ELLQPTAIYLQNSDDAFKEVVKTVLNEDIPESDDSASFDATRQFTPEELEELQRRVDE
                     SNKELEAYQLDSQSDSTTEPEVNLSETQTFKLNI"
     gene            7148..9949
                     /locus_tag="VGT87_000995"
     CDS             7148..9949
                     /locus_tag="VGT87_000995"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEQ3813862.1"
                     /translation="MKLKETLNLGKTAFPMRAGLPNKEPQWQAAWEQAELYKKRQELN
                     AGKPAFHLHDGPPYANGNIHVGHALNKISKDIIVRSKSMSGFQAPYVPGWDTHGLPIE
                     QVLAKQGIKRKEMDLAEYLEMCRQYALSQVDKQRNDFKRLGVSADWENPYVTLDPQFE
                     ADQIRVFGAMAEKGYIYRGAKPVYWSWSSESALAEAEIEYHDIDSTSLYYANKVKDGK
                     GILDTNTYIVVWTTTPFTVTASRGLTVGPDMDYLVVKPAGSDRQYVVAEGLLNSLAGK
                     FGWESFETLASHKGADLEYIVTEHPWDTDVEELVILGDHVTLESGTGIVHTAPGFGED
                     DYNVGTKYKLEVAVTVDERGLMMENAGPDFHGQFYNKVTPIVIDKLGDLLLAQEVINH
                     SYPFDWRTKKPIIWRAVPQWFASVSDFRQDILDEIEKTTFHPSWGETRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTKEVTDHVADLFQENGSIIWWQKEAKDLLPEGF
                     THPGSPNGEFTKETDIMDVWFDSGSSWNGVMNARENLSYPADLYLEGSDQYRGWFNSS
                     LITSVAVNGHAPYKAILSQGFVLDGKGEKMSKSKGNIISPNDVAKQYGADILRLWVAS
                     VDTDNDVRVSMEILGQVSETYRKIRNTLRFLIANTSDFNPATDTVAYADLGAVDKYMT
                     IVFNQLVATITDAYERYDFMAIYKAVVNFVTVDLSAFYLDFAKDVVYIEAANSLERRR
                     MQTVFYDILVKITKLLTPILPHTTEEIWSYLEHESEAFVQLAEMPVAETFSAQEDILE
                     AWSAFMTLRTQAQKALEEARNAKIIGKSLEAHLTIYASEEVKTLLTALDSDIALLLIV
                     SQLTIADLADAPADAVAFEGVAFMVEHAIGEVCERSRRIDPTTRMRSYNAFVCDHSAK
                     IIEENFPEAVAEGFEESGK"
     gene            complement(10214..10516)
                     /locus_tag="VGT87_000996"
     CDS             complement(10214..10516)
                     /locus_tag="VGT87_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEQ3813863.1"
                     /translation="MRLINTTSSHPELIRNQLKNTDAYLVEVYSAGNTDVIFTQAPKH
                     YELLISNKYRAIKEDELDIIREFFLKRKIDPKIVIPGQSKTLHTNNLIEISFQTSV"
     gene            complement(10567..11022)
                     /locus_tag="VGT87_000997"
     CDS             complement(10567..11022)
                     /locus_tag="VGT87_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEQ3813864.1"
                     /translation="MMIPTFGHKNAHKDYVTRYGVYAIIPNHEQTKIILVQAPNGSWF
                     LPGGEIEAGEGQLQALERELIEELGFSATIGSYYGQADEYFYSRHRDTHFYHPAYLYE
                     VTAFQAVSKPLEDFNNLGWFSPIEAIAKLKRESHQWGVKEWQKKHHSTN"
     gene            complement(11150..13432)
                     /locus_tag="VGT87_000998"
     CDS             complement(11150..13432)
                     /locus_tag="VGT87_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEQ3813865.1"
                     /translation="MLCQNCNLNESTIHLYTSVNGKQRQVDLCQNCYQIMKSDPANSI
                     LNGLTPGYRAQDRSTSPFFDDFFGDLNNFRAFGNLPNTPPTQAGQNGNGGGRYGGNYN
                     GQRPAQPQTSNQQAKGLLEEFGINVTDIARNGNIDPVIGRDEEITRVIEILNRRTKNN
                     PVLIGEPGVGKTAVVEGLAQKIIDGTVPQKLQGKQVIRLDVVSLVQGTGIRGQFEERM
                     QKLMEEIRNRKDVILFIDEIHEIVGAGSAGDGNMDAGNILKPALARGELQLVGATTLN
                     EYRIIEKDAALERRMQPVKVDEPSVEETITILKGIQPKYEDYHHVKYSPAAIEAAAHL
                     SNRYIQDRFLPDKAIDLLDEAGSKMNLTLNFVDPKEIDKRLIEAENLKAQATRDEDYE
                     RAAYFRDQITKYKEMQAQKVDEQDIPIITEKTIEAIVEQKTNIPVGDLKEKEQSQLVN
                     LANDLKAHVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLA
                     IELFGSTNNMIRFDMSEYMEKHAVAKLVGAPPGYVGYEEAGQLTEQVRRNPYSLILLD
                     EVEKAHPDVMHMFLQVLDDGRLTDGQGRTVSFKDTIIIMTSNAGTGKSEASVGFGAAR
                     EGRTSSVLGELSNFFSPEFMNRFDGIIEFKALSKEHLLHIVDLMLEDVNERLGYNGIH
                     LDVTQKVKEKLVDLGYDPKMGARPLRRTIQDYIEDAITDYYLEHPTEKQLRALMTNSE
                     NITIKAVKEGDSFLNEESLD"
     gene            13730..13960
                     /locus_tag="VGT87_000999"
     CDS             13730..13960
                     /locus_tag="VGT87_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003105222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEQ3813866.1"
                     /translation="MESHLVRIINRLELMVTDGGNLKRNFERDGVVVAEVAFSNDPEN
                     GPVFTLRDVEARESYSFDSIDLIAMEIYDLLY"
     gene            14087..14773
                     /locus_tag="VGT87_001000"
     CDS             14087..14773
                     /locus_tag="VGT87_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEQ3813867.1"
                     /translation="MDLSFLPKYWAYFNYGVLVTIMISVSVVFFGTLIGVLVTLIKRS
                     HVKPLTWVVNLYVWIFRGTPMVVQIMIAFAWMHFNNMPTIGFGVLDLDFSRLLPGIII
                     ISLNSGAYISEIVRAGIEAVPKGQLEAAYSLGIRPQNAMRYVILPQAFKNILPALGNE
                     FITIIKDSALLQTIGVMELWNGAQSVVTATYSPISPLLVAAFYYLMVTTVMAQLLAVL
                     ERHMSQGGNH"
     gene            14773..>15499
                     /locus_tag="VGT87_001001"
     CDS             14773..>15499
                     /locus_tag="VGT87_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEQ3813868.1"
                     /translation="MTEAIISIKDLHKYYGHNEVLKGIDLDIMPGEVVVIIGPSGSGK
                     STLLRTMNLLEVPTKGQIRFEGIDITDKKNDIFSMREKMGMVFQQFNLFPNMTILENI
                     TLSPIKTKGMAKAEADKTALSLLDKVGLSEKAKAYPASLSGGQQQRIAIARGLAMDPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAKEVADRVIFMDGGVI
                     VEEGSPNQLFDLTKEERTKDFLSR"
BASE COUNT         4801 a         2699 c         3271 g         4728 t
ORIGIN      
        1 ttttatgatc actccttata gcaaaaagaa agagttttct gtaagaggaa accatcaaac
       61 gaaccttgac gaattaatca aagctagcaa agtcaaagca tctgactatt ggttaacgtt
      121 gttaacttcg cctggtcagt atgaacgacc gattcttcgt actattccat gggtgaaatc
      181 tgtacatctc tcttaccaat ttcctaatca ctttctattt aacgttattg aatttgaaat
      241 catcgcttat gcacaagtcg aaaacggttt tcagcctatt ttggagaatg gaaaacgtgt
      301 tgacaaggtc agggcatcag aactaccgaa atctttcttg attcttaatt taaaagatga
      361 gaaagcgatc caacagttag ttaagcaatt aacgacatta cctaaaaaat tagtcaagaa
      421 tatcaagtca gtgtctcttg caaattccaa aacgacagcg gatttactac ttattgaaat
      481 gcatgacggt aatgtagtta gagtaccgca gtcacaactc acattgaaac ttccctatta
      541 tcaaaaattg aaaaaaaacc ttgaaaatga tagtatagtg gatatggaag ttggaattta
      601 tactacaaca caggagattg aaaatcaacc tgaagttcct cttacgccag aacaaaacgc
      661 agctgataaa gaaggagata agcctggtga acatcaggaa cagacagaca atgattcaga
      721 gacgccagca aatcagagta gtcctcagca aacaccacca tccccagaaa cggtcctcga
      781 acaggctcat ggctagctaa tatctaagtt gaaaaagcaa tgaaaacgtt agaaattcaa
      841 cgattctaac ccataatgaa ttgcctaaaa aaaattaagt ttatataaca aaaaacgtaa
      901 aatgataaca ttttacgttt ttttatggta taatattttc tgaatgattc tgttttttag
      961 cagtttttag aatagcaaaa gtttggaaag taagtgaggt caagtgaatg gctagaaatg
     1021 gcttttttac tggtttggac attggaacaa gctcgataaa agttttagta gcagaattta
     1081 tttctggtga gatgaacgtc attggtgtta gtaatgttcc aagtaccggc gtaaaagatg
     1141 gcataataat cgatatagag gcagctgcga ctgccatcaa aactgcggta gaacaagcag
     1201 aagaaaaagc agggatgaca attgaaaagg ttaatgttgg gctaccggca aaccttcttc
     1261 aaattgaacc aacacaagga atgattcctg tcccaagtga gtctaaagag ataaaagatg
     1321 aggatgttga tagcgttgtt aaatcggctt taacaaaaag tatcacacca gaacgagagg
     1381 ttatctcttt agttccagaa gagttcattg tggatggctt tcagggcatt cgagatccac
     1441 gtggtatgat ggggattaga ttagagatgc gcgggcttat ttatactgga ccaagcacca
     1501 ttttacataa tctgcgtaaa acggtagaaa gagcaggcat taaagttgaa aacatcatta
     1561 tttctccgtt agctatggct aaaaccattt taaacgaagg tgagcgcgag tttggagcta
     1621 ctgtaattga tatgggaggt ggacagacaa ctgtcgcttc tatgcgagca caagaattgc
     1681 agtataccaa tatatatgct gaaggcggcg aatacattac taaagatata tcaaaagtat
     1741 taaaaacgtc tttggctatt gcagaagcac ttaagtttaa ttttggtcaa gcggagatat
     1801 cagaagctag tataactgaa acagtaaaag ttgatgtggt aggtagtgaa gagcctgttg
     1861 aggtaactga acgttattta tctgaaatta tttcagcgcg tattcgtcat attttagatc
     1921 gtgtgaagca agatttggaa agaggtcgtt tactagactt accaggaggc attgttttga
     1981 ttggcggcgg tgcaatcatg cctggagtgg tagagattgc acaagaaatc tttggagtaa
     2041 ctgtaaagct ccatgttcca aatcaagtcg gtattagaaa tccaatgttt tcaaacgtta
     2101 tcagtttggt agaatatgtt ggtatgatgt ctgaagtaga cgttttagca caaactgcag
     2161 tttcaggaga agaacttttg cgacgcaagc ctatcgattt cagtggtcaa gaatcttatt
     2221 taccagatta tgatgattca agaagaccag aatcgaccat tggctatgaa caacaagcgt
     2281 cacaaacagc atatgattca caagttccga gtgatcctaa acaaaaaatt tcagaacgtg
     2341 ttcgtggcat atttgggagt atgtttgatt aaaagtaata aagtgaggag ataaaatggc
     2401 attttcattt gatactgcat caattcaagg tgcaattata aaagtaattg gagtcggcgg
     2461 aggtggcgga aatgccatta atcgtatgat tgatgaaggt gttgctggtg tcgagttcat
     2521 cgcagcaaac acagacattc aggcattaag ctcatcaaaa gctgaaacgg ttattcaact
     2581 aggccctaaa ttaactcgtg gacttggtgc tggaggacaa cctgaagtag gacgtaaagc
     2641 tgctgaagaa agcgaagaaa ttttaacaga agctcttaca ggagcggaca tggtatttat
     2701 tactgccggt atgggtggtg gctctgggac aggggctgca ccggttattg ctcgtatcgc
     2761 taaaagtttg ggagccttga cagtagctgt tgttactcgc ccgtttggtt ttgaaggtaa
     2821 caaacgtggt aattttgcta ttgaaggtat cgaagaactc cgtgaacaag ttgatacttt
     2881 gttaattatt tcaaataata accttcttga gattgttgat aaaaagacac ctttattaga
     2941 agcacttagt gaagctgata atgttttacg tcagggagtt caagggataa ccgacttaat
     3001 tactagtcct ggccttatca atctcgattt tgccgacgtg aaaacagtta tggcaaataa
     3061 agggaatgcc ttgatgggga ttgggattgg ttctggagaa gagcgcattg ttgaggcggc
     3121 gcgtaaggca atctattcac ccctattaga aacgactatt gatggtgcac aagacgttat
     3181 tgtgaacgtt acaggaggtc tcgacatgac actcacagaa gctgaagaag cctctgaaat
     3241 tgttgggcaa gctgctggtc aaggcgttaa catttggtta ggaacatcta ttgatgatac
     3301 tatgaaagat gacatccgtg tgactgttgt agcaactgga gtgcgccaag aaaaagccga
     3361 acaagtttca ggttttcgtc agcctaggac ttttacccaa accagcgcgc agcaagtagc
     3421 gggtgcacaa tatgcatcag atcaagcaaa acagtcggtt caaccagggt ttgatcgtcg
     3481 ctcaaatttt gattttgaca tgggggagtc tcgcgagata ccaagtgcac aaaaggtaat
     3541 ttctaatcat aatcaaaatc aaggttcagc ttttggaaat tgggatttga gacgtgataa
     3601 tatttctcgt ccaacagaag gtgaattgga taaccatctt aatatgtcaa cgttctcagc
     3661 taacgatgac agtgatgatg aattagaaac gcctccattc tttaaaaacc gttaataatg
     3721 gatttactga caaataaaaa gaaaattttt gagactatcc gcttatctac agaggcagca
     3781 aataggacta atgatagtgt ttcagttatt gctgtaacaa aatatgtgga tagtacaatt
     3841 gcaggtcagc ttatcgaagc aggaattgag cacattgccg aaaaccgtgt tgataaattt
     3901 cttgaaaagt atgatgcgtt aaagtatatg ccagtaaagt ggcatttaat tggtacctta
     3961 caacgtcgta aagtcaagga agttatcaat tatgtcgatt attttcacgc tctagattct
     4021 gtgagattag ctttggaaat caacaagaga gctgaccatc ctgtgaagtg ttttctacaa
     4081 gttaatattt ctaaagaaga gagtaaacat ggttttaaca tttctgagat tgatgaagcg
     4141 attgaagaaa taggtaagat ggagaagata cagttagttg gtttaatgac tatggcacca
     4201 gcaaatgcca gtaaaggaag tattataact atttttcgac aagcaaatca attaagaaaa
     4261 aacttgcagt taaaaaaaag aaagaatatg ccttttacag aattgagcat gggcatgagt
     4321 aacgattatc caattgctat tcaagaaggc tcaactttta ttcggattgg tagagctttc
     4381 tttcactaat ggagaataag atggctttta aagatacatt taacaagatg atttcttatt
     4441 ttgacacgga tgaggttaac gaagttgaag aagatgttgc agcatcaact gataacgtta
     4501 ttccaagatc acaacaatca gtcagagcaa gtagtcatcc aaaacaagaa cctagaaaca
     4561 atcacgtaca acaagatcat caagcgagat cccaagaaca gacaaggtca caaatgcatc
     4621 caaaacatgg tacttctgaa cgctattatc agcagtctca gccaaaagaa ggccatgaaa
     4681 tggttgacag aagaaaacgg atgaacactt ctggtattgc aaatcgccgt gagcagtatc
     4741 aacaatcaac ttgttcagat caaacaacta ttgccttaaa gtatcctcgt aaatatgagg
     4801 acgctcaaga aattgtggat cttttaatag ttaatgaatg cgttttgatt gattttcagt
     4861 ttatgctaga tgctcaggct agacggtgtt tagattttat tgatggtgct agtaaagtgc
     4921 tctatggtag cttacaaaag gtcggctctt caatgtactt actggctccg tcaaatgtat
     4981 ccgtcaatat agaagaaatg actatcccac atactacaca agatattggc tttgattttg
     5041 atatgaaaag gcggtaaata aatgatatta atactatcta ttcttcttcg tctgatcaaa
     5101 gtttacactt atttattgat tgcttacgca ctaatgtcat ggtttcctgg ggcatacgat
     5161 tcaaaaattg ggcgtttgat tagtggtatc gttgaaccaa ttttaaaacc ttttagagca
     5221 tttaatttac aatttgccgg tcttgacttc actatttttg tcgtcattat tagtttgaat
     5281 tttttagctc aagttttggt ccgtgtgttt atttaatggt tagtcatagt aagatttatc
     5341 agcattttca ccaagaagaa tatcctttta ttgatagaat gtctgatatg attaatagag
     5401 ttgaagatta ctatctttta gaagttactg agtttttaaa tcctagagaa gtgatgattt
     5461 taaaaagttt gattgcttta acagatctaa aaatgttcgt atcaacagat tactacccaa
     5521 gcgaatatgg tcgtgtcatt attgcacctg gttactatga cttagaacaa agtgattttc
     5581 aaatagcttt agtagagata agttatcagg caaagtttaa tcagttgaca catagtcaaa
     5641 ttttaggaac tttaattaat gaattaggag taaagcgaaa tttatttgga gatgtttttg
     5701 ttgaaatggg atatgcccag ctcatgatta agcgggagtt attggattat tttttaggaa
     5761 caattactaa aatagctaaa actagtgtga aattaagaga agttaacttt gatcagttaa
     5821 ttaggtctat tgataacagc cagaccctgg atattctagt ttctagtttt cgattagatg
     5881 gtgtagttgc tactatctta aaaaaatctc gaacgcaagt tatagcatta attgaagcaa
     5941 ataagattaa ggtaaactat cgactcgcta ataaagcttc agataatcta gtcatagggg
     6001 atatggtgag tatcagaggt cacgggcgtt ttactctttt agcagataat ggagtgacca
     6061 aacatggcaa acaaaaaata acactaagta aaatgataca taaataagga gatacaatgg
     6121 cacttacaac gctagaaatt aaagataaaa cttttaagac aaaattccga ggatactgtg
     6181 aagaagaagt caatgaattc cttgatattg ttgttgatga ttacgaagct cttgtacgta
     6241 aaaatcgtga taacgaagca agaattaaag atcttgaaga aaaattatct tactttgatg
     6301 aaatgaaaga gtcacttagc cagtctgtaa tcttagctca agaaacagct gaaaaagtga
     6361 aagctacggc taatgcggaa gcaacaaact tggttagtaa agcgacttat gatgctcagc
     6421 atttattaga tgaatctaaa gctaaagcca atcaaatgtt gcgtgatgca actgatgagg
     6481 ctaagcgagt tgcgattgaa acagaagaat taaagcgtca aacacgtgtg ttccaccaac
     6541 gtttaatctc atctattgag tcacaattaa gcttatcaaa ttcacctgaa tgggatgaac
     6601 tgctacaacc gactgcaatc tatcttcaaa attctgacga tgcttttaag gaagtcgtga
     6661 agaccgttct taatgaagac attcctgaat ctgatgatag tgcctctttt gatgcaacac
     6721 gtcagtttac tccagaagag ttagaagaat tgcaacgtcg tgttgatgaa agcaataagg
     6781 agttagaggc ttaccaactt gactctcaat ctgattctac gactgagcca gaggtaaatc
     6841 tcagtgaaac acaaacgttt aaattaaata tctaaataaa gaaaaacaag atgatttgct
     6901 tataatcaca gcaagcggga gatgatgaga gtctcgtatt ccctaagtat tatcattatc
     6961 attttctaaa attttcgctg aactacagta agcgaggagg tttgttcacc ttacgagcaa
     7021 agagggagcg gtaagtctta tgatatatta tcgctctgaa ctaaggtggt accacgagct
     7081 ttcgtccttt ttgagacgag gcttttttcg ttatagtcgc aagtttgttt aaaaggagtt
     7141 tgaaataatg aaattaaaag agacacttaa tctaggaaaa actgcctttc caatgcgtgc
     7201 aggtcttcct aataaagagc cacaatggca agcagcttgg gaacaagcag agctttacaa
     7261 aaaacgtcaa gaattgaatg caggtaagcc tgcctttcat cttcatgatg gacctcctta
     7321 cgctaacggc aatattcacg ttgggcatgc ccttaataaa atttcaaaag atatcattgt
     7381 gcgttctaag tccatgtctg gctttcaagc gccttatgta ccaggttggg acacacacgg
     7441 ccttccaatc gaacaagtct tggctaagca aggaatcaaa cgtaaggaga tggatttagc
     7501 agaatacctt gagatgtgtc gtcaatacgc tttaagtcag gttgataaac agcgaaatga
     7561 tttcaaacgg ttaggtgttt cggcagactg ggaaaatcct tatgtcactt tggacccaca
     7621 gtttgaagct gatcaaattc gtgtttttgg agcgatggct gaaaaaggtt acatctatcg
     7681 tggtgctaaa cctgtttatt ggtcatggtc ttcagaatcg gccttggcag aagctgaaat
     7741 tgaataccat gacatcgatt caacctcttt atactacgcc aataaagtta aagatggtaa
     7801 aggcatcctt gatactaata cgtatatcgt tgtttggacc acaacaccat ttaccgttac
     7861 agcatcacgc ggtttgactg ttggtcctga tatggattat cttgtggtga aacctgctgg
     7921 ttcagaccgt caatatgttg tggcagaagg tcttttgaat agccttgctg gaaaatttgg
     7981 ctgggaatct tttgaaactt tagccagcca taaaggagct gatttagagt acattgtgac
     8041 agaacaccca tgggatactg acgtagaaga attggttatc cttggtgacc atgttaccct
     8101 tgagtcaggg acagggattg tccacacggc gccaggtttt ggtgaggatg actacaatgt
     8161 tgggacaaaa tacaaactgg aagttgctgt gactgttgat gaacgtggcc taatgatgga
     8221 aaatgcaggt ccagatttcc acggacaatt ttataacaag gtaacgccga ttgtcattga
     8281 taaacttggt gatcttttat tggcacaaga agtgatcaat cactcttacc catttgactg
     8341 gcggacgaaa aaaccaatca tctggcgtgc tgtaccgcaa tggtttgctt ctgtttctga
     8401 tttccgtcaa gatattttgg atgaaattga aaaaacaacc ttccatccat catggggtga
     8461 aacacgcctt tacaatatga ttcgtgatcg tggcgactgg gttatttctc gtcaacgtgc
     8521 ttggggagtt ccgcttccta tcttctatgc cgaagatggc acagctatta tgactaaaga
     8581 agtgacagat catgtggctg atttgttcca agaaaatgga tcaatcattt ggtggcaaaa
     8641 agaagctaaa gacctattac cagagggctt cactcatcca gggtcaccaa atggcgagtt
     8701 tacaaaagaa actgatatca tggacgtttg gtttgactct ggttcttctt ggaatggagt
     8761 catgaatgct agagaaaatc tttcctatcc agcggatctc tacttagaag gctctgacca
     8821 gtatcgtggt tggttcaatt catccttaat tacatctgtg gctgttaatg ggcatgctcc
     8881 atataaggct attttatcgc aaggctttgt tcttgatggt aagggcgaaa aaatgtctaa
     8941 atcaaaaggc aatatcattt ctcctaatga tgtggctaaa caatatggtg cagatattct
     9001 tcgactttgg gtagcttctg tggataccga caatgatgtc cgtgtttcta tggagatcct
     9061 tggtcaagtc tctgaaacct accgtaaaat ccgtaacacc cttcgtttct tgattgctaa
     9121 tacatctgat ttcaaccctg ctacggatac agtagcttat gcagacttag gagctgttga
     9181 taagtacatg acaattgtct ttaatcagtt ggtagcaacg attactgacg cttatgagcg
     9241 ttatgacttt atggctattt acaaagcagt ggtgaacttt gttacggttg atttatcagc
     9301 cttctatctt gattttgcta aagacgttgt ctatattgaa gctgccaata gcttagaacg
     9361 tcgtcgcatg cagacagtct tctatgatat cttggttaag attaccaagt tattgacgcc
     9421 aatcttgcct cacaccactg aagaaatctg gtcttatcta gaacatgagt cagaagcatt
     9481 tgttcaattg gcagaaatgc ctgtggcaga aactttctca gctcaagagg atattttaga
     9541 agcttggtct gctttcatga ctttgcgtac ccaagctcaa aaagctttgg aagaagcgcg
     9601 aaatgctaag attatcggta aatcattgga agcccatttg accatctacg ctagcgaaga
     9661 agtgaaaacc ttattgactg ctttagacag cgatattgct ttgcttttga tcgtgtctca
     9721 attaaccatt gctgacttgg cagatgcgcc tgcggatgca gtggcatttg aaggtgttgc
     9781 ctttatggta gaacatgcca taggtgaagt ttgtgagcgt tcacgtcgca tcgacccaac
     9841 tactcgcatg cgttcttaca atgcatttgt ctgtgatcac agcgctaaaa tcattgaaga
     9901 aaatttccca gaagctgtgg ctgaagggtt tgaagagagt ggcaaataag tcttatcaag
     9961 acttgaagtg acactgagta agaagacaac catcgggtct taggacctga tggtttttta
    10021 gtagagctga ttacgtccat tagccagctt taacgagaga caaagtagga gaaatttaga
    10081 ctactttttt aatttctttt aagaaaattg ctggcttcat ataaggacta ttaagataga
    10141 gttcaccttt cgctaataaa aaaacttagc tcttttagaa gggctaagtt ttgtctgaat
    10201 agctggctgt tgtttataca gaagtttgaa atgaaatttc aataaggtta ttcgtgtgaa
    10261 gtgtttttga ttgtccagga ataacaattt taggatcaat tttacgtttt aaaaaaaatt
    10321 cgcgaatgat atcaagttca tcctccttga tggcacggta tttatttgaa ataagtagtt
    10381 cgtaatgttt tggtgcttgg gtaaaaatga catctgtatt ccctgctgag tatacttcga
    10441 ctaaataggc atcggtgttt tttagctgat ttctgatgag ttctgggtga ctgctagtgg
    10501 tattgatgag tctcataaat gtcctcctta gtattcctgt acctattata gcactaattt
    10561 aaagccttag ttagtagaat gatgcttttt ttgccattcc ttgactcccc attgatggct
    10621 ctctcgtttt aatttagcaa ttgcctcgat aggagaaaac caacctaagt tattaaaatc
    10681 ttctaagggc ttagaaacgg cttgaaatgc agttacttca taaagatagg caggatggta
    10741 gaagtgggta tcacgatggc gagagtagaa atattcatca gcctgaccgt aatatgaacc
    10801 aatagtagca gaaaaaccaa gttcttcaat cagttcacgc tctagggcct gaagctgacc
    10861 ttcacctgct tcaatttcac caccaggtaa aaaccaggaa ccattaggag cttgaacaag
    10921 aataattttt gtttgttcgt gattgggaat gatagcatag acaccgtaac gtgtgacata
    10981 gtctttatgt gcatttttat gtccaaatgt tggaatcatc attttattta cctttccttt
    11041 ttcttcatta tactaaattt ttgctaatta ttcttatcaa tttgtttagt ttaataaaaa
    11101 attgatagaa aaagagagag tctccttata gaaactctct tagctaacgt taatcgagag
    11161 actcttcgtt aagaaaagaa tctccctctt taacagcttt aatcgtgata ttttcactgt
    11221 ttgtcatcag tgcacgtaac tgtttttcag ttgggtgctc taaataatag tctgtaatgg
    11281 catcttctat ataatcttgg atagtacgac ggagcggccg agcgcccatt ttaggatcat
    11341 agcctaaatc tactaatttt tctttgacct tttgtgtcac atcaagatga atcccattat
    11401 agcctaagcg ctcattaaca tcttccagca ttaaatcaac gatatgcagc aaatgctctt
    11461 tagataaagc tttgaattca ataataccat cgaaacgatt cataaactct gggctgaaga
    11521 agttgcttaa ttcaccaagg acagaacttg tccgtccttc tctagcagca ccaaatccga
    11581 cagaagcttc gcttttacct gttccggcat tagaggtcat gataataatg gtgtccttga
    11641 agctgactgt tcgtccttgg ccgtctgtta agcgaccgtc atcaagaact tgtaagaaca
    11701 tgtgcatgac gtcaggatgg gctttttcca cctcatctaa gagaataagt gaatatggat
    11761 tgcgacgaac ttgttcggtt agctgtccag cttcttcata gccgacataa cctggaggag
    11821 ccccgactaa tttggcgaca gcgtgttttt ccatgtattc ggacatgtca aagcgaatca
    11881 tattgtttgt cgaaccaaag agttcaatgg ctagttgttt agacaattca gttttaccaa
    11941 ccccagtcgg tccaacgaat aagaaagaac caatagggcg atttggagtt cctaatccca
    12001 cacggttacg acgaatagcc ttagcgattt tatcaacagc atcgtcttgc ccgatcacgt
    12061 gtgctttcag atcattggct aagtttacga gttgagactg ttccttttct tttaagtccc
    12121 caactggaat attagttttt tgctctacaa tagcttcaat ggttttttca gtaatgatgg
    12181 gaatatcttg ctcatcgact ttttgagcct gcatttcttt gtattttgta atttgatcgc
    12241 ggaaataagc tgcgcgttca tagtcttcgt ctcgagtagc ttgcgccttg agattctcag
    12301 cttcaataag acgtttgtca atttctttag gatcaacaaa gttgagggtt agattcattt
    12361 tggaaccagc ttcgtccaga agatcaatag ccttatcagg aagaaaacgg tcttgaatat
    12421 agcggttaga taaatgagcg gcagcttcaa tagcggctgg gctatatttg acatgatgat
    12481 agtcttcgta tttcggttgg atacctttta aaatcgtgat ggtttcctca acagaaggtt
    12541 catcaacttt aacgggttgc atacgtcgtt ctaaggcagc gtctttttca ataatacggt
    12601 attcatttaa tgtagtagca ccaacgagtt gcaactcacc acgggccaag gctggtttta
    12661 aaatattacc agcatccata ttgccgtctc ctgcagaacc agcaccgaca atctcatgaa
    12721 tttcatcaat aaagagaatc acatccttgc gattgcgaat ttcttccatt aatttttgca
    12781 tgcgctcttc aaactgacca cggatacctg ttccctgaac gaggctaacc acatcaagac
    12841 gaatcacttg cttgccttgg agtttttgag gaacagtacc atcaataatt ttttgagcca
    12901 aaccttctac aacagcagtt ttaccaactc caggctcacc aattagcaca ggattatttt
    12961 tagtacggcg gttgaggatc tcgataacgc gtgtaatctc ttcgtcacga ccaataacag
    13021 gatcaatatt accatttctt gcaatatctg tgacattaat cccaaactct tctaacaagc
    13081 cctttgcttg ctgatttgat gtttgtggct gagcaggtcg ttgtccgttg tagttaccac
    13141 catagcgtcc tccgccattt ccattttgcc ctgcctgagt aggtggggta tttggaagat
    13201 taccaaaagc tctaaaatta ttcaaatcac caaaaaagtc atcaaagaaa ggacttgtgg
    13261 atctatcttg tgctctatat cctggggtta ggccatttaa aatagaattg gcaggatcgg
    13321 atttcatgat ttgataacaa ttttgacaga gatcaacctg tctttgtttt ccattaacac
    13381 ttgtataaag atgaatagta gattcgttta aattacaatt ttgacacagc ataatatacc
    13441 tccgagggtt tatctaaaag tcaaagtcat ttagtcaaaa atggtcaaac tttatattca
    13501 tattatagca ccacggcttg ggaaatcaag taaaaagttt atcgctttag cgttagcttt
    13561 tagagagtaa catgacagag taactttatt aagtttccaa tgagcaaatg tttaaaatca
    13621 ttgaaaagag taaaaaaagt aaaatatcaa taaatatatt actttccttg ttagtttccc
    13681 ttgttttgtg ttaaaataag agggataaaa aacgaatagg agaaacgaaa tggaatcaca
    13741 tttggtgaga atcatcaatc gtttggagtt gatggtgaca gatggcggta atctcaagcg
    13801 aaattttgaa cgcgatggtg ttgttgttgc agaagttgcc ttttcaaatg atcctgaaaa
    13861 tggacctgtt ttcacattgc gtgatgttga agcgcgtgag tcatattcct ttgatagcat
    13921 tgacttgatt gcaatggaaa tctacgattt attatattaa gcttgacata gtaaagagac
    13981 ttgcctgagg caggtttttt tattgtcaaa aagtattgta ttaatattca tcagatggta
    14041 taattatctt tattgtaatg aacctaggac ttatgaggag atataaatgg atttgtcatt
    14101 tttgcccaaa tactgggcct actttaacta cggtgtactt gtcaccatta tgatttcagt
    14161 cagcgttgtc ttttttggaa cccttattgg tgtcttggta accctgatta agcgtagtca
    14221 tgtgaagccg ttgacctggg tcgttaatct ttacgtttgg atctttcggg gaacacctat
    14281 ggtggttcaa atcatgattg cctttgcttg gatgcatttt aacaatatgc ctactattgg
    14341 ttttggggtt ttagacttgg acttttcaag actacttcct ggaattatta tcatttcatt
    14401 gaatagcggt gcttatattt cagaaattgt tagagcaggt attgaggctg taccaaaagg
    14461 gcaattagaa gcagcttatt cactaggtat tcgtcctcaa aatgccatgc gttatgtgat
    14521 tttgcctcag gcctttaaaa atattttgcc agccttagga aatgaattta ttaccattat
    14581 taaggatagt gctcttttac aaaccattgg agtgatggaa ctttggaatg gtgcccaatc
    14641 ggtggtaacg gctacttatt ctccaatttc ccctttactg gtggctgctt tttactactt
    14701 aatggtcaca acagtgatgg cacagttatt ggcagtctta gaacgtcaca tgtcgcaagg
    14761 aggtaatcat tgatgactga ggctattatt tcaattaaag acttgcacaa gtattatggg
    14821 cacaatgaag tgcttaaggg aattgattta gacattatgc caggagaggt tgtggtgatt
    14881 attggccctt ctggttctgg aaaatcgacc ttacttcgaa ctatgaatct tttggaagta
    14941 ccaactaagg gacagattag gtttgagggg attgatatta ccgataagaa gaatgatatt
    15001 ttcagcatgc gtgaaaaaat gggaatggtt ttccagcagt ttaacctctt tcccaatatg
    15061 actattttag aaaatatcac tttatcgcca atcaaaacta agggaatggc taaagcagag
    15121 gctgacaaaa cagccttgag cttgttggac aaagttggat tatcagaaaa agccaaggct
    15181 tatcctgcta gtctttctgg tgggcaacag cagcggattg cgattgcgcg tggactggct
    15241 atggatccag atgttttact ctttgatgaa ccaacttcag ctctagaccc agaaatggtg
    15301 ggtgaggtct tggctgtcat gcaagatttg gctaaatctg ggatgactat ggttattgtg
    15361 actcatgaaa tgggctttgc taaagaagtc gcagatcgtg tgatttttat ggatggcggc
    15421 gttattgtgg aagaaggaag tcctaaccaa ctttttgatc tgactaaaga agaacgtaca
    15481 aaagattttt tgagtagag
//
LOCUS       DAUCLR010000037        15120 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.037, whole genome shotgun sequence.
ACCESSION   DAUCLR010000037 DAUCLR010000000
VERSION     DAUCLR010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15120
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.037"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(18..902)
                     /locus_tag="VGT87_001002"
                     /pseudo
     CDS             complement(18..902)
                     /locus_tag="VGT87_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354842.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            915..1481
                     /locus_tag="VGT87_001003"
                     /pseudo
     CDS             915..1481
                     /locus_tag="VGT87_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631865.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
     gene            1496..2488
                     /locus_tag="VGT87_001004"
     CDS             1496..2488
                     /locus_tag="VGT87_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEQ3813869.1"
                     /translation="MKLKLYNVRGEEAVLAKKWADDNGIEISLTESSLTPETVKEAEG
                     FDGIANAQIGPLDDAIYPLLKEMGIKQIAQRSAGVDMYNLDLATENDIIITNVPSYSP
                     ESIAEFTVTIALNLIRHVELIRENVKKQNFTWRLPIRGRVLGDMTVAIIGTGRIGLAT
                     AKIFKGFGCKVVGYDIYQSDAAKAVLDYKESVEEAIKDADLVSLHMPPTAENTHLFNA
                     DLFKSFKKGAILMNMARAALIETQDLLDALDAGLLSGAGIDTYEFEGPYIPKNFEGQE
                     ITDSLFKALINHPKVIYTPHAAYCTDEAVKNLVEGALNATVEIIKTGTTTTRVN"
     gene            2593..3258
                     /locus_tag="VGT87_001005"
     CDS             2593..3258
                     /locus_tag="VGT87_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HEQ3813870.1"
                     /translation="MTYIALIGDIIQSKQLTDRSKVQKTLAAYLDDLNKTFAPYIISK
                     LSLTLGDEFQGLFQVDTPIFHLIDLINHHMDIPIRFGVGVGSILTDINPDISIGADGP
                     AYWHAREAIRYIHQKNDYGNTTLALRTGHHNQDDVLNSLLAAGDAIKANWRASQWEIF
                     DTLLDLGIYEEYFDQQRLGKQLSLSSSALSKRLKSSHVKIYLRTRQSALNCLKQIKEE
                     TDD"
     gene            3251..3967
                     /locus_tag="VGT87_001006"
     CDS             3251..3967
                     /locus_tag="VGT87_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HEQ3813871.1"
                     /translation="MINGVSHYLAQTPTLTLFLICHVLSDYQLQSQQVADLKEKHLTY
                     LGYHLIGVSIPLICLTLIIPQAWLMSLLVMISHALIDWLKPKMANSLKWKREWIFLLD
                     QCLHIAISSFAGLRLAGVTLPNWLPISILMTVLFILLITKPTNIVFKLFFSKYQPDQG
                     EKMDTIIGAGATIGILERIVIGVCMIMGQFASIGLVFTAKSIARYNKISESPAFAEYY
                     LIGSLFSILSVFIAAWICFF"
     gene            4118..5464
                     /gene="trmFO"
                     /locus_tag="VGT87_001007"
     CDS             4118..5464
                     /gene="trmFO"
                     /locus_tag="VGT87_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEQ3813872.1"
                     /translation="MSQSTATYINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKATP
                     QHKTTNFAELVCSNSFRGDSLTNAVGLLKEEMRRLDSIIMRNGEANRVPAGGAMAVDR
                     EGYAKSVTAELENHPLIEVIRDEITEIPNDAITVIATGPLTSDALAEKIHAVNGGDGF
                     YFYDAAAPIIDKSTIDMSKVYLKSRYDKGEAAYLNCPMTKEEFMAFHEALTTAEEAPL
                     NAFEKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQ
                     LRQDNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLL
                     TETFQSRSNPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKREEALIFPQTTAIGS
                     LPHYVTHADSKHFQPMNVNFGIIKELEGPRIRDKKERYEAIASRALADLDTCLASL"
     gene            5617..7023
                     /locus_tag="VGT87_001008"
     CDS             5617..7023
                     /locus_tag="VGT87_001008"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HEQ3813873.1"
                     /translation="MIQFGGFSMQQQVAITETVLRDGHQSLMATRLSIEDMLPVLTIL
                     DKIGYYSLECWGGATFDACIRFLNEDPWERLRTLKKGLPNTRLQMLLRGQNLLGYRHY
                     ADDIVDKFISLSAQNGIDVFRIFDALNDPRNIQQALRAVKKTGKEAQLCIAYTTSPVH
                     TLNYYLSLVKELVEMGADSICIKDMAGILTPKAAKELVSGIKAMTNLPLIVHTHATSG
                     ISQMTYLATVEAGADRIDTALSPFSEGTSQPATESMYLALKEAGYDITLDETLLEQAA
                     NHLRQARQKYLADGILDPSLLFPDPRTLQYQVPGGMLSNMLSQLKQANAESKLEEVLA
                     EVPRVRKDLGYPPLVTPLSQMVGTQAAMNVILGKPYQMVSKEIKQYLAGDYGKTPAPV
                     NEDLKRSQIGSAPVTTNRPADQLSPEFEVLKAEVADLAQTDEDVLTYALFPSVAKPFL
                     TTKYQTDDVIKVTAFIKA"
     gene            7066..7380
                     /locus_tag="VGT87_001009"
     CDS             7066..7380
                     /locus_tag="VGT87_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813874.1"
                     /translation="MTYSFIDILQVTLVSLFLVLTCLTGIMGLMMLSGKLLSQEKKTL
                     SQQASSIPQEQEADKLSGEALETLLAKDELARIAVMTSLAYASQEESGKRFEVIAVER
                     KS"
     gene            7380..7730
                     /locus_tag="VGT87_001010"
     CDS             7380..7730
                     /locus_tag="VGT87_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEQ3813875.1"
                     /translation="MKRYEVTVNGQVYEVSLRELADGETVEVSRPAAPATEKEMNANA
                     AGGGIQVKAPMSGTVLSIFATEGKAVKKGEAVLVLEAMKMENEILAPADGLVSKIHVV
                     ANQTVESEQVLISF"
     gene            7740..8870
                     /locus_tag="VGT87_001011"
     CDS             7740..8870
                     /locus_tag="VGT87_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HEQ3813876.1"
                     /translation="MLDVLNQMVQSSGLAHLTVNNLIMICLACFFLYLGIKKEYEPYL
                     MVPIAFGILLVNLPMAGLMDHPANGNPGGLLYYLYKGTSLGIYPPLIFLCLGASTDFG
                     PLIANPKTILLGGAAQVGIFLAFFLAIMLGMTPQEAASVGIIGGADGPTAIYVTTKLA
                     PDLLSTIALAAYSYMALVPIIQPPIIKLLTTKAERQVKMTQARTVSQKEKIIFPIMVT
                     IFVSLLVPSATTLVGCLMLGNLVREIKIVPKIVENLQQVVMFCITIILGLTVGAKANG
                     DLFLSATTLKIIALGLIAFAAGTAGGVLMGKIMYYLSGNKVNPMIGAAGVSAVPMAAR
                     VVQKIGQEEDPSNFLLMHAMGPNVAGVIGSAIASGALLAFFG"
     gene            complement(9070..9954)
                     /gene="citG"
                     /locus_tag="VGT87_001012"
     CDS             complement(9070..9954)
                     /gene="citG"
                     /locus_tag="VGT87_001012"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HEQ3813877.1"
                     /translation="MTKAVLTSISQLALKALLYEVSLSPKPGLVDRFDNGAHDDMSFM
                     TFIDSMIALSPFFQAYIETGFAYAKEEPLLLFNRLRQLGQKAEETMFCATQGINTHKG
                     LNFSMALLLGATGAYLARTPHLMTDLGRFSKEDTLAICRLVKPMTAHLIQTDLGHLNT
                     KKEFTYGEQLFVTYGIKGPRGEASEGFTTLTDHALPYFRQMISQNDPETSQLRLLVYL
                     MSIVEDGNLIHRGGIEAWKGVKADMRLLLQQDLSTTDLRLALSSYNQCLINQHLSPGG
                     AADLLALTFYFAFLEKLL"
     gene            complement(9947..10642)
                     /locus_tag="VGT87_001013"
     CDS             complement(9947..10642)
                     /locus_tag="VGT87_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ3813878.1"
                     /translation="MNPIIEAVKQNLDLSRNIPLKIAFYNALKKTIILRQIPVGSRIN
                     EKEFSIALNISRTPIRYALGLLSEEHLVEHIPKKGIIVKGVSIKDACEIFEIRKALET
                     LATVQAMHLMTEEDFKVMHNLLEDCETFIAEDDTNRILDNFNAFNNLIYSYSQMVRLK
                     EIVTELQAYLVYFRKISISSVERRKRALSEHWMIYRGMKNKDHEQITLITHEHLNSSL
                     EFILKEMRPEQND"
     gene            complement(10800..12203)
                     /locus_tag="VGT87_001014"
     CDS             complement(10800..12203)
                     /locus_tag="VGT87_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HEQ3813879.1"
                     /translation="MLLTMLAYAMIIVFMYVVMKKKMTPFTALVMIPLIMTIAVILTG
                     SADFNADAKFVAFVGDGGIAKDLTAIGPMVMYGINNTAKTGIMLLFAILFFSVMLDAG
                     LFDPITEKMIRFAKGDPMKVLIATAVVAAAVSLNGDGTTTTLICCSAFLPIYKKLDMK
                     IMNLGVLIILQNTIMNLLPWGGPTARAMSVLGVGPEILGYLAPGMILSLLYVICWVAP
                     SMGRKERARLGVIDLSEEDMRQLTDITDPDTLFIRRPKNFVFNAILTIGLITWLVAGS
                     FNKSIAMAPLLLFAVGTCIALMVNYPVLKDQSKRIGDNAGDAVQVVILVFAAGIFMGL
                     FQGSGMASALAQSFATIIPKQLAGFWGLVIALVSAPGTFFISNDGFYYGILPVLAEAG
                     AEYGFSNMAMALASLMGQAFHLLSPLVAFIYLLLRLTGLDMGEWQKEAAKYALIIFVI
                     FVVTIIAMGQMPLYIPQ"
     gene            12583..12906
                     /locus_tag="VGT87_001015"
     CDS             12583..12906
                     /locus_tag="VGT87_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813880.1"
                     /translation="MQFLQKWFKHSKKEKLKESSPLSTEIEPSENWEKIPAYIPADKS
                     DYKKVTLITSVIAAGDRPNSQFKVKRILKRNPEAITVSLIASSIAAGVYPESQFRVTS
                     IYCKR"
     gene            12963..13358
                     /locus_tag="VGT87_001016"
     CDS             12963..13358
                     /locus_tag="VGT87_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HEQ3813881.1"
                     /translation="MLRKFKITIDGKEYLVEMEEIGAPAQAAAPAQPISTPVSVPTEA
                     SPQVEEAQAPQPVAAAGADAIPSPMPGTILKVLVAVGDQVTENQPLLILEAMKMENEI
                     VALSAGTITAIHVGPGQVVNPGDGLITIG"
     gene            13403..14524
                     /locus_tag="VGT87_001017"
     CDS             13403..14524
                     /locus_tag="VGT87_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019323213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HEQ3813882.1"
                     /translation="MDTLIAGITSITIPQIVMMVIGALLMYLGIKKEYEPTLLVPMGL
                     GTILVNFPGSGVLTQVVNGVEQEGVFEALFNFGIGTELFPLLIFIGIGAMIDFGPLLQ
                     NPFMLLFGAAAQFGIFFVVVVAVLAGFDIKEAASIGIIGAADGPTSIFVANQLAKDLL
                     GPITVAAYSYMALVPIIQPFAIKLVTTKKERRIRMTYKAENVSQMTKILFPIIITLVA
                     GFIAPISLPLVGFLMFGNLLRECGVLDRLSQTAQNELVNIISILLGLTISIKMQADLF
                     LNVQTLLIIVFGLLAFIMDSIGGVMFAKFLNLFRKEKINPMIGAAGISAFPMSSRVIQ
                     KMATDEDPQNFILMYAVGANVSGQIASVIAGGLLLAYFS"
     gene            14712..15020
                     /gene="citD"
                     /locus_tag="VGT87_001018"
     CDS             14712..15020
                     /gene="citD"
                     /locus_tag="VGT87_001018"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HEQ3813883.1"
                     /translation="MDIKQTAVAGSLESSDLMITVSPNDEQTITITLDSSVEKQFGNH
                     IRQLIHQTLVNLKVTAAKVEAVDKGALDCTIQARTIAAVHRAAGVDQYDWKEIDSWNV
                     "
     gene            15008..>15120
                     /locus_tag="VGT87_001019"
     CDS             15008..>15120
                     /locus_tag="VGT87_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625899.1"
                     /note="citryl-ACP lyase; catalyzes the formation of
                     acetate and oxaloacetate from citrate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit beta"
                     /protein_id="HEQ3813884.1"
                     /translation="MERLRRTMMFVPGANAAMLRDAPLFGADSIMFDLEDSV"
BASE COUNT         4616 a         2950 c         3049 g         4505 t
ORIGIN      
        1 tttaactaca aaaatcgcta gttcgttttt aaaaggctta aaaattcctc aataactgga
       61 ctgaaactcg catcatctcg gtaagcaatc ccaatagggt aaaaattatt tttatcttgc
      121 aaaggaatcc aaactaagtt atctacctga ttaagactct caaaatcact cgggaggata
      181 gtcaaggtat ttaaatcgtg caaactatga ataagaactt cccaatcatt gtgtttaaag
      241 acaatatctg gttcaaaacc gagagctcgg cattttctag gaagcatttc gcctaacatg
      301 tagtgatcgt ttaaagaagc aatcttatag ccttttaaat ctttaagttt taactgagga
      361 tgttgcgcca aagggtgctg tttgaggaga acgatactga ccttatagcc ttttgtggac
      421 gtttgtaata gctcaatggt aatatccttt tgaatactta gaaaggagag gagaccaata
      481 tcgatttttc cttgggacaa taattcttat aatttgcgag aaccatcctg tataagtgat
      541 aattcaacat ttgagtgagt agccataaag attgaaattt gtttcatgaa ttgtatagca
      601 aacagtgatg ttaattcaac acgaatcatc tctttttttc ttgatttaat tgctgaatat
      661 ccgcaacaat ttgattaaag tcatccacaa ggtctttgcc tttgtgataa agaatatctc
      721 cagcttcggt tagtagtagc tgtcaatcac tttgtgtaaa aagcgtcgtg ttcaaactag
      781 gactctagcc ttttaattga ttgagaaaga gtatgttgcg ttacaaacaa acttttagca
      841 gcgtgggaat gattttttgt ttcagcaact gcaataaaat aggttaatta tctaatatcc
      901 atagtagatc cttcatgatt ggtcagggaa ttgcgtctct acttggttta caacctattt
      961 tgatgtctct ccttattgcc atgattttct gcttccttat tgtttctcca attacaacgg
     1021 taggaattgc tcttgcgatt aacttatctg gtattggttc aggtgctgca aacttaggta
     1081 tttgtgccgc aagctttgga ctttgcttag caggttgggc tgttaattca aaaggaactt
     1141 cacttgctca cgtcttggga tcaccgaaaa tttcaatggc caacgtttta tcaaaaccaa
     1201 aaatcatgtt gccaatgtta tgttctgcag ctgtacttgg tgttattggt gctatcttta
     1261 atattcaagg aacacctgct agtgcaggct ttgggattag ttgtttgatc ggacctatca
     1321 atgcccttaa tttagctaaa ggcggatggt ctctagtaaa tatcctactt atcattatca
     1381 tttttgtaag ggcaccaatt gttctcaata tgatctttaa ctaccttttt atcaaggtgc
     1441 ttaaagtgat tgatccaatg gattacaaac ttgatatcta agaaaggaac ttttgatgaa
     1501 attaaaatta tataatgtta gaggcgaaga agcagtctta gcaaaaaaat gggctgatga
     1561 caatgggatt gagatttcct tgacagagtc ttccttaact cctgaaacgg ttaaagaagc
     1621 tgaaggcttt gacgggattg ccaatgccca aattggtcct ttagatgatg cgatttatcc
     1681 tctcttaaag gaaatgggga ttaaacaaat cgctcagcgc agtgctggtg tcgatatgta
     1741 taaccttgat ttggccaccg aaaatgacat tattatcaca aatgttccta gctattctcc
     1801 agaatccatt gcagaattta cggttactat tgcccttaat ttaattcgtc atgtggagtt
     1861 gattcgtgaa aatgttaaaa aacaaaattt cacttggaga ctccctatcc gcggccgtgt
     1921 tttaggtgat atgacagttg cgattatcgg aactggccgt attggtctag ctactgctaa
     1981 aatctttaaa ggttttggct gcaaggtcgt tggatatgat atttaccaaa gcgatgctgc
     2041 caaagctgtc ttggactata aggaatctgt agaagaagcg attaaagatg ctgatcttgt
     2101 ttccttacac atgccaccaa ctgcagagaa cacacacctt ttcaatgctg atttatttaa
     2161 atcattcaaa aaaggggcta ttttgatgaa tatggcgcgt gctgctctca ttgagacgca
     2221 agatttactg gatgctttgg atgcaggctt actgagcgga gctggtattg acacttacga
     2281 atttgaagga ccttatatac ctaagaattt tgaaggtcaa gaaattacag attcactatt
     2341 taaagctttg attaaccatc ctaaggttat ttatacgcct catgcagctt attgtactga
     2401 tgaagccgtt aaaaacctag ttgaaggtgc ccttaatgct acagtagaga ttatcaaaac
     2461 tgggacaacg acaactcgtg ttaactaaca actgatttga cgtccattat aaaggaataa
     2521 ttttaaaaat tcctttataa tggacttttt tgcattagtc cgttataata gactgtaaga
     2581 aaggggactc ttatgacata tatagcgctt attggagata ttatccagtc caaacaattg
     2641 acggatcgtt ccaaagttca aaaaacttta gcagcttatt tagacgacct caacaaaacc
     2701 tttgctcctt atattatttc taaattatcc ctaacgttgg gagatgaatt tcaaggactt
     2761 tttcaggtgg atactcctat tttccatctg attgatttga ttaatcatca catggatatc
     2821 cctattcgat ttggagtagg agtaggttct attctaacag acatcaatcc tgatattagt
     2881 attggggctg atggacctgc ttattggcat gctcgtgagg ctattcgtta tattcatcaa
     2941 aaaaatgatt atggtaatac tacattagct cttcggactg gtcatcacaa ccaggatgat
     3001 gtattaaaca gtttactagc agctggagat gctattaaag ccaactggcg agctagtcaa
     3061 tgggaaattt ttgacacgct tttagatttg ggcatttatg aggaatactt tgatcaacaa
     3121 cgactaggaa agcaattatc tcttagctct agcgctttgt caaaacgtct aaaaagtagt
     3181 catgttaaaa tttatctgag aacacggcag agtgcgctaa actgtctcaa acaaatcaag
     3241 gaggaaactg atgattaatg gtgtctctca ctacctagct caaaccccta cacttaccct
     3301 gtttctcatt tgtcatgtgt tatcagacta ccagttacaa agtcagcaag tagctgattt
     3361 aaaagaaaaa catttgactt atttaggtta ccacttgatt ggagttagca ttcccctaat
     3421 ttgtttaacc ctcattatcc cacaagcatg gttgatgagc ctattggtta tgataagtca
     3481 cgcgcttatt gactggctaa agccaaagat ggctaactct ctaaaatgga aaagagagtg
     3541 gatttttctg ctggatcaat gcttacatat cgctattagt tcatttgctg ggctaaggtt
     3601 agcaggggtg accttgccta attggcttcc gatatctatt ttgatgaccg ttttatttat
     3661 cctactcatt accaaaccaa ctaatattgt ctttaaactc tttttcagca aataccagcc
     3721 tgaccaagga gaaaaaatgg acacgatcat aggagcgggg gctaccatag gaattctaga
     3781 gagaattgtt attggggtgt gtatgatcat ggggcaattt gcttcaatag gtctggtctt
     3841 taccgctaaa tctattgctc gctataataa aatttcggaa agtccagctt ttgcagaata
     3901 ctacctaatt ggttctttat ttagtattct cagtgttttt atcgcagcat ggatttgttt
     3961 cttctagttg aaaagggttc ccgtagaaag aggctaacgc ttagtctctt tctttttctt
     4021 tcttcttatt tctgttataa taagtcaggt aactacatta gaaagatttg tgggtctgtc
     4081 atctaagaga tgtcattccc gaagaggtat tagtgacttg tctcaatcaa ctgcaactta
     4141 tattaatgtt attggagctg gactagctgg ttctgaagct gcctatcaga ttgctaagcg
     4201 cggtatcccc gttaaattgt atgaaatgcg tggtgtcaaa gcaacaccgc aacataaaac
     4261 cactaatttt gccgaattgg tctgttccaa ctcatttcgt ggtgatagct taaccaatgc
     4321 agtcggtctt ctcaaagaag aaatgcgtcg cttggattcc attattatgc gtaatggtga
     4381 agctaatcgt gtacctgctg gaggagcaat ggctgttgac cgtgaggggt atgcaaagag
     4441 tgtcactgca gagttggaaa atcatcctct cattgaggtc attcgtgatg aaatcacaga
     4501 aattcctaac gatgccatta cggttatcgc gacgggaccg ctgacttcgg atgccctggc
     4561 agaaaaaatt cacgcggtaa atggtggcga tggattctat ttctacgacg cagcagcgcc
     4621 tatcattgat aaatctacta ttgatatgag caaggtttac ctcaaatctc gctacgataa
     4681 aggcgaagct gcttacctca actgccctat gaccaaggaa gaattcatgg ctttccatga
     4741 agctctgaca accgcagaag aagccccgct gaatgctttt gaaaaagaaa agtattttga
     4801 aggctgtatg ccgatcgaag ttatggccaa acgaggcatc aaaaccatgc tctatggccc
     4861 catgaagcca gtcggcttgg aatatccaga tgactataca ggtcctcgcg atggagaatt
     4921 taaaacgcca tatgccgtcg tgcaattgcg tcaagataat gcagctggaa gcctttataa
     4981 tatcgttggt ttccaaaccc atctcaaatg gggtgagcaa aaacgcgttt tccaaatgat
     5041 tccagggctt gaaaatgctg agtttgtccg ctacggcgtc atgcatcgca attcctatat
     5101 ggattcacca aatcttttaa cggaaacctt ccaatctcgg agcaatccaa accttttctt
     5161 tgcaggtcag atgactggag ttgaaggtta tgtcgaatca gctgcttcag gtttagtagc
     5221 aggaatcaat gctgctcgtt tgttcaaaag agaagaagca cttatttttc ctcagacaac
     5281 agctattggg agtttgcctc attatgtgac tcatgccgac agtaagcatt tccaaccaat
     5341 gaacgtcaac tttggcatca tcaaagagtt agaaggccca cgcattcgtg acaaaaaaga
     5401 acgttatgaa gctattgcta gtcgtgcttt ggcagattta gacacctgct tagcgtcgct
     5461 ttaacactaa aaaagagttc cttttcagtt gagttgctga agaggacttt tttacttgct
     5521 gatttgagcg ctagctatgc aaaagaatgc acaaaaaata catgaataga ctatggtcac
     5581 tagaagcaat attatataat gaatagtact cattagttga ttcagtttgg aggttttagc
     5641 atgcagcagc aagtcgcaat aacagaaaca gttttacggg acggtcatca aagtttgatg
     5701 gcaacgcgtt tatctattga ggatatgctt cctgtcctta ccatcttaga caagattggt
     5761 tactattccc tagagtgttg gggaggagca acctttgatg cctgtattcg ttttttgaac
     5821 gaagaccctt gggaaaggct gcgcacgtta aaaaaaggct tacctaatac ccgtttacaa
     5881 atgttattaa gaggccaaaa ccttttaggc taccgtcatt atgcagatga catcgttgac
     5941 aaattcatta gtttatcagc tcaaaatggg attgatgttt tccgcatttt tgatgcccta
     6001 aatgacccac gaaatatcca gcaagcctta agagctgtga aaaagacggg taaagaggct
     6061 caactgtgta ttgcctatac aactagccca gtgcacacgc ttaactatta cctttcttta
     6121 gtgaaagaat tggttgagat gggagctgat tctatctgca tcaaagatat ggctggtatt
     6181 ctaactccca aagcggctaa agagttagta tctggtatca aagccatgac taacttacct
     6241 cttattgtgc atacccatgc aactagtggc atttctcaga tgacctatct agcaacagta
     6301 gaagctggtg ctgatagaat tgatactgct ttgtcacctt ttagcgaagg tacttcacaa
     6361 cctgctacag aatccatgta tttagcctta aaagaagcag gttatgatat cacattagat
     6421 gaaacgctac tggaacaggc tgctaatcac cttcgacaag cccgtcaaaa gtacttagca
     6481 gatggcattc tggatcccag tcttctcttt ccagacccaa ggacactaca ataccaagtt
     6541 cctggaggca tgttatctaa tatgctatct cagctgaaac aagccaatgc cgaatcaaaa
     6601 ttagaagaag ttttagcaga agttcctcgg gtaagaaaag acttgggtta tcctccctta
     6661 gtaacaccgc ttagccaaat ggtcgggact caagctgcta tgaatgtgat tttaggaaaa
     6721 ccttaccaga tggtgtctaa ggaaattaaa caatacttgg caggcgacta tggcaaaaca
     6781 ccagcaccag tcaacgaaga cttaaaacga agtcaaattg gctcagctcc tgtcaccaca
     6841 aatcgtcctg cagatcaact aagtcctgaa ttcgaagtgt taaaagcaga agtagcagat
     6901 ttagcccaaa cggacgaaga tgtgctgact tatgccctct tcccaagcgt agcaaaaccg
     6961 tttttaacaa ccaaatacca aactgatgac gtcattaaag tgactgcctt tatcaaagca
     7021 taataggcta tcttaatcag atagaaacaa aaatatggag atcacatgac ctattccttt
     7081 attgatatct tacaagttac cttagtatcc ttgttcttag tgttaacctg tttaacaggg
     7141 attatgggat taatgatgct aagtggtaaa ttattgtcac aagaaaaaaa aactctctct
     7201 caacaagctt ccagcattcc tcaagagcaa gaagctgaca agttgagtgg agaagctctc
     7261 gaaactcttt tagccaaaga cgaattggct agaattgctg ttatgactag tcttgcttat
     7321 gctagccaag aagagtcagg aaaacggttt gaagttattg ccgtggaaag gaaatcctaa
     7381 tgaaaagata tgaagtgact gtcaatggac aggtttatga agtgagttta cgtgaactag
     7441 ctgatggaga aactgttgaa gttagccggc cagcagcacc tgctactgag aaagaaatga
     7501 atgccaacgc tgctggcggt ggcattcagg taaaggctcc aatgtctgga acagtcttgt
     7561 ctatctttgc tacagaagga aaagctgtta aaaaaggaga agctgttctg gtcttagagg
     7621 ccatgaaaat ggagaatgaa attctagcac ctgcagatgg tctggtttca aaaattcatg
     7681 ttgtggctaa ccaaacggtc gagtctgaac aagttttgat ttcattctag gaggaaacca
     7741 tgctagacgt actcaatcaa atggttcaat cgtcaggatt ggctcatcta actgttaaca
     7801 atcttatcat gatttgcttg gcctgtttct ttttatacct tggtatcaaa aaggaatatg
     7861 agccttacct tatggtacca atcgcctttg ggattttact cgttaatttg cctatggcgg
     7921 gattgatgga ccaccctgcc aatggtaatc ctggtggcct tctttattac ctatataagg
     7981 gaactagcct agggatttac ccacctttaa tttttctttg tttgggggca tcaactgact
     8041 ttggtccctt gattgccaat cctaaaacca ttttactagg aggcgcagca caagttggga
     8101 ttttcttagc ctttttcctt gctattatgt taggcatgac acctcaagaa gcggcttctg
     8161 tcgggattat tggtggtgcc gatggaccaa cagctattta tgtgacgaca aagttggcgc
     8221 ctgatttact ctctactatc gccctagcag cttattctta catggctctg gtacctatta
     8281 ttcaacctcc catcattaaa ctcttgacta ccaaggcgga acgccaagtg aaaatgactc
     8341 aagcaagaac tgtttcacaa aaagaaaaaa tcattttccc gattatggta acaatctttg
     8401 tcagcctttt ggttccaagt gctacaacct tggttggctg tttgatgctg ggaaatctgg
     8461 tcagagaaat taaaattgtt cctaaaattg tagagaatct ccaacaagta gttatgtttt
     8521 gcattacgat cattttagga ttaaccgttg gtgctaaggc caatggagat ctctttttat
     8581 cagccacaac cttgaaaatt attgcccttg ggctaatcgc ctttgctgct gggacagcag
     8641 ggggtgtctt gatggggaaa atcatgtact atttatctgg taacaaagtg aaccctatga
     8701 taggagcagc aggtgtttca gctgttccga tggcagcacg agtggtccaa aagatcggac
     8761 aagaagaaga tccaagtaat ttcttactca tgcacgccat ggggcccaat gtagctggcg
     8821 ttattggctc agccattgcc tcaggtgcct tgctggcctt ctttggctaa ttgtgaaaaa
     8881 ggattagtaa attaatctta ataaatcatt atcaggagtt ggaagcaatc tagctccttt
     8941 ttagtagaaa gctcgagtga taaactattc caaaataatt ataaaaaaag actgtcctga
     9001 ttgtacggac tatatcaatt gacttttata gtctagctga aatagcagtg caaaactaag
     9061 acactcgttt taaagtagtt tttctaaaaa agcaaaataa aaggtcagag ccaataaatc
     9121 tgccgcgccc ccagggctta aatgctgatt aatgaggcat tgattgtagg aggacaaagc
     9181 caatcttagg tcagttgttg ataaatcttg ttgcaggagt agtcgcatat ctgccttaac
     9241 gcctttccat gcttcaatcc ctccacgatg aatcagattt ccatcttcaa caatagacat
     9301 caaataaaca agaagacgaa gctgactggt ttcggggtca ttttgactta tcatctgacg
     9361 aaagtagggc aaggcatggt ctgttaaggt cgtgaatccc tcgcttgcct caccccgagg
     9421 tcctttgatg ccataagtta caaagagttg ctccccataa gtgaactctt ttttagtgtt
     9481 caaatgacca aggtctgttt gaatcaaatg tgccgtcata ggttttacta aacgacagat
     9541 agctagagta tcttccttgc taaaacgccc taaatcagtc attaagtgag gtgttcttgc
     9601 tagataggcc ccagttgcac caagaagtaa agccatggaa aaattcaagc ctttatgagt
     9661 gttaattccc tgagtagcac aaaacatggt ttcctcggcc ttttgaccta gttgtctaag
     9721 acggttaaac agaagaagtg gctcttcttt tgcataagca aaacctgttt cgatataagc
     9781 ctgaaaaaaa ggagagagtg ctatcatact gtcgataaag gtcataaagg acatgtcatc
     9841 atgggcaccg ttatcaaatc gatcaactaa accaggtttt ggagatagtg agacttcata
     9901 aagcaaagct tttaaggcca gttgactaat acttgttaga actgccttag tcattttgct
     9961 caggcctcat ttcttttaag ataaattcaa gtgagctatt caagtgttca tgtgtaatca
    10021 acgtaatttg ctcatgatct ttgttcttca tacctcggta aatcatccag tgttcagata
    10081 gagctcgttt acggcgttca actgaagaaa tcgaaatttt tctaaagtag accaaataag
    10141 cttgcaattc tgtcacaatt tcttttaagc ggaccatttg gctatagctg taaatcaaat
    10201 tgttaaaggc attaaaatta tccaaaatac gattggtgtc atcctctgcg ataaaggttt
    10261 cacagtcttc caacaagtta tgcatgactt taaaatcttc ttctgtcatt aaatgcatcg
    10321 cttgcaccgt tgcgagagtc tctagagctt ttcgaatttc aaaaatttca caggcatcct
    10381 tgatactaac tcctttgaca ataatgcctt ttttaggaat gtgctctaca aggtgttctt
    10441 cagacaataa tcccaaagcg tagcgaatag gtgtcctgct aatattaagc gcaatggaaa
    10501 actctttttc attaatgcgg cttccaacag gaatttgtcg caaaataatg gtctttttaa
    10561 gagcgttata aaaggctatt ttcaaaggga tatttcttga taggtcaaga ttttgtttaa
    10621 cggcttcgat aataggattc atgtcatctc cttaatgcta gtgtcctcac tttattatac
    10681 ttaaatttgt gaaacattcc aataggacta gatgagttct gaaaaattaa agccatgctc
    10741 acaaaaggac atgactttaa tcatctcata aggaataagg tagtagtgtt atcgttggtt
    10801 tattgtggaa tgtaaagtgg catttgtccc atggcaataa tggttaccac aaagataaca
    10861 aagatgataa gggcatattt agcagcctct ttttgccatt cccccatgtc aagacctgtc
    10921 aagcgaagaa gaagataaat gaaggcaact aatggactaa gtaagtggaa ggcttgtccc
    10981 atgagggaag caagtgccat agccatgtta ctgaaaccat attcagcacc agcttctgca
    11041 agaacaggca agataccata gtaaaaacca tcattggaga taaagaaggt accaggtgca
    11101 gaaactaagg caataacgag cccccagaaa cctgccagtt gttttggaat aatggttgca
    11161 aaactttgtg caagagcgct agccatacca gaaccttgga aaagacccat aaagatacca
    11221 gcagcaaaga cgagaataac cacttgaacc gcatcaccag cattatcacc aatgcgtttc
    11281 gattgatctt taagcactgg gtaattgacc atcaaggcga tacaagttcc caccgcaaaa
    11341 agaagaagcg gtgccatagc aatagatttg ttgaaagagc cagcaactaa ccaagtgatt
    11401 aatccaatgg ttaagatagc attgaaaaca aagtttttag gacgacgaat aaaaagggta
    11461 tctgggtcag taatgtctgt gagttgacgc atgtcttctt cagacaagtc aataacacca
    11521 agtcttgcac gttctttacg ccccatgctt ggagcaaccc aacaaatcac ataaagaaga
    11581 gataaaatca tacccggtgc aagatagcca agaatttcag gaccgacacc aagaacagac
    11641 atcgcacgag cagtaggacc accccatggc agtaagttca taatagtatt ttgaagaata
    11701 atcaagacac ctaggttcat gattttcatg tccaattttt tatagatagg taagaaagca
    11761 gagcaacaaa ttaaagtagt ggttgttcca tcaccattaa gagatactgc ggcagcaacg
    11821 acagctgttg cgataagcac cttcattgga tcacctttag caaagcgaat catcttttca
    11881 gtaatcggat caaacaatcc tgcatctaac atgacagaga agaacaaaat ggcaaatagc
    11941 aacatgatac ctgttttagc agtattgttg ataccataca taaccattgg tccgatagct
    12001 gttaggtctt tagcaattcc accgtcacca acaaaggcaa caaatttggc atctgcattg
    12061 aagtcagctg aaccagtcag tataacagca attgtcatga ttaatggaat catgaccaaa
    12121 gcagtaaaag gggtcatttt tttcttcatt accacgtaca taaagacaat aatcatggca
    12181 taagccagca ttgttaataa catcattgtt atctcctttt tcaagttatg atttcagtat
    12241 agatgaaagc gcttataaaa taaatacttc tttgtgcaga aagacacaaa aaaaacaatt
    12301 tgaaaattaa ttttgaaaac catttttatt acatcagatg actttaaatt ttctataata
    12361 atgatgaacc tagttagatc aagcttttct tgctatcagt atcttgatct cagcttttca
    12421 acagttacct tttattttgt ttttaggtaa ctgttgaaaa gctttatgaa acattgaaag
    12481 cgctcttaga aagaacacaa aaaagccatc tcaaaaaaga ttggtagtat ataataaact
    12541 tactagttcc ctaaataggt aaatgtgaaa aagaggtagc tcatgcagtt tttacaaaaa
    12601 tggtttaaac actctaaaaa agagaagttg aaagaatcct cgccccttag tacagaaata
    12661 gaaccttctg aaaactggga aaagattccc gcttatattc ctgcagacaa aagtgattac
    12721 aaaaaggtta ctttaattac cagtgttatt gctgctggcg atagacctaa tagtcaattc
    12781 aaggttaaac gcattctcaa gcgcaatcct gaggcaatca ctgtttcttt aattgcttca
    12841 agtatcgctg caggtgttta cccagaaagt cagtttcggg tgacatccat ctattgcaaa
    12901 aggtagtcgg tgaacgatac tttagacgaa ctcatgacac aataacatat aggagaaaaa
    12961 tgatgttacg aaaatttaaa attaccattg acggtaaaga atatcttgta gaaatggaag
    13021 aaatcggtgc tcctgcccaa gcagctgccc ccgctcaacc aatatccact ccagtatctg
    13081 tccctactga agcaagccca caagtggagg aagcgcaagc accacagcca gtagctgcag
    13141 cgggagcgga tgccattcca tcaccaatgc ctggaaccat cttaaaagtc ttagtagcag
    13201 tgggagacca agtaactgaa aatcagccac tattgatttt agaagccatg aagatggaaa
    13261 atgagattgt ggccttatca gcaggaacca ttacagctat ccacgtcggt ccaggtcaag
    13321 tggtcaatcc tggtgatggt ctcattacaa ttggttaata gtactataac tagaataaat
    13381 agacagaaaa ggaagtgaat ttgtggacac tttaatcgca ggaataacct ccatcaccat
    13441 tcctcaaatt gttatgatgg tcatcggtgc cctcttaatg tatctaggaa ttaaaaaaga
    13501 atacgaacca accttacttg tccccatggg acttggaacc attctcgtta acttccctgg
    13561 ttcaggagtt ttaacccaag tggttaacgg agtcgagcaa gaaggggttt ttgaggctct
    13621 cttcaatttt gggattggaa cagaactttt cccactgttg attttcattg gtataggagc
    13681 catgattgac tttggtccct tgcttcaaaa cccattcatg ctcttgtttg gtgctgcagc
    13741 tcaattcgga attttctttg ttgtcgttgt tgccgtactg gctggctttg acattaaaga
    13801 agcagcttca atcggcatta tcggagcagc agacggaccg acttctatct ttgttgccaa
    13861 ccaactggcc aaggatttac tgggtccaat tacggttgca gcctattctt acatggctct
    13921 ggttcctatt atccaaccat ttgccattaa attagtgaca acgaaaaaag agcgccgtat
    13981 ccgtatgact tacaaagctg agaatgtatc tcaaatgact aaaatcttat tcccaatcat
    14041 cattacctta gtagcagggt tcatcgcccc aatttctcta cctttagttg gctttttgat
    14101 gttcggtaac ttattacgtg agtgtggtgt gctcgatcgc ctgtcacaaa ctgctcaaaa
    14161 tgaattggta aacattatta gtatcttgct tggattgacc atctctatta aaatgcaggc
    14221 agacctgttc ttaaatgtac agacactctt aatcatcgtc tttggtttac ttgcttttat
    14281 catggattct atcggtgggg tcatgtttgc caaatttctc aatctcttcc gaaaagagaa
    14341 gattaatcca atgattggcg ctgcgggtat ttcagctttt ccaatgtcaa gtcgtgtcat
    14401 tcaaaaaatg gcaacagatg aagacccgca aaactttatc ctcatgtacg ctgtaggtgc
    14461 caatgtctct ggccaaatcg cctctgttat cgcaggtggt ttgttgctag cttactttag
    14521 ctaagaaagg aaggaaacct tatgacaatt aatatggacc atttgattag ttcctttgag
    14581 ctaatggcgc ttggtatggc aggtgtcttt atcgtgttag gcattctcta tcttgtcgca
    14641 gagatcctga tcaaactttt cccagttagt aaataacaat atcatttaag aaaatcaagg
    14701 aggtaaacgc aatggatatt aaacaaactg ccgttgctgg ttcacttgaa tcaagtgacc
    14761 taatgattac agtatcccct aatgacgagc aaaccattac aataacccta gacagtagtg
    14821 ttgaaaaaca atttggcaat cacattcgtc aactcattca tcaaacccta gtgaatttga
    14881 aggtgacggc tgctaaagta gaggctgttg ataaaggcgc tttagattgc accattcaag
    14941 cgagaaccat cgcagctgtt catcgcgcag ctggtgttga ccaatacgat tggaaggaga
    15001 ttgactcatg gaacgtttaa gaagaacaat gatgtttgtg ccaggtgcca atgcagccat
    15061 gcttcgagat gctcctttat ttggcgctga ttcaatcatg tttgacttgg aagattctgt
//
LOCUS       DAUCLR010000038        14997 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.038, whole genome shotgun sequence.
ACCESSION   DAUCLR010000038 DAUCLR010000000
VERSION     DAUCLR010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14997
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.038"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            143..3217
                     /locus_tag="VGT87_001020"
     CDS             143..3217
                     /locus_tag="VGT87_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEQ3813885.1"
                     /translation="MTKGIRFQLLGSPHIFLDDKEQFFAFAKANALLYYLVVNGSVSR
                     EVAASLLWENKNTQTAKKNLRNAIYQVNKVLQADVIICPNRNLLVLNKTLDIKTDINL
                     FLADPLAHLELYQGEFLQGFYLKSGEEFDLWVSKMRMQYEQVYLKACYQKIEEKLSLD
                     AIEDVEEHLKQLIERDEFEEKNYQLLMRLYQQGNCPGKVIETYYQLANVLDKELGIQP
                     SLQSQQIYQEVVAKDRNERKIKHFLRNSNHFLGRIDEIKQLENYFANCLACQEVGALL
                     LIGDTGIGKRTLARQVLANQTQTFQIVTAKCFREEAMDSLLPWRNILDGLGDLVIQNR
                     LLTTKAWKAALKRCFPAATIFQEDNNQPFIKDHTSLLVSFIVDILQHLAEIKALVILI
                     EDCHWMDEDSLTLLQRVMNQLVHYPIAFVLTKHLGTTPELGLCLNALMSQGRLESICL
                     EPFNRQESLAYISSQLGSQPVTEEEMEHLYQASQGNPFFLSEYTQALLRHEKFVPLTP
                     AIKAKLGLKLANLSSRDDALLNYLSCCRRPIPLNTLAQLMLLPLEEVIEMVDNLGHYY
                     ILVEESVGEEVLISFRQRIIQLYSYYRLSLSKRRLLHGQIAKRLEDLLPILTPSPHLL
                     DDIAYHYQESRQVIKALEYNLNYLDATLPFQHELFPIYSKSIGSLEKSDRDHQRLMEE
                     QFDKIRQSIADLELTYDNNRDFQQLLIRFSYLEGRYDIRTGRYQEGIKHIQKVIALAT
                     ELKQPSFLLEGYRQLIHYCIQVENKPEMRYYTSLSLEAAVAANHFEAIAISLRLNGLY
                     HLIIGELNEAERLLQQSIDFFKVTPGLQANYAIQIAAALDYLGEIAQIRYQFEKAVTY
                     QKQAIALTENKPAELSVSIFYIGLGISYFYLADFEQAEQILSLAKEALVNHSYPWKET
                     QLEIYLAMIQWKKGNYQPALTLLDYRETLMSRYRNPRDEGLVFYLMAVVKYQLIFQGA
                     TLSQQEKEMADHLLSESFEYYYEIASTNLNPYRDCHLVSELNDLRQQLSAKS"
     gene            complement(3248..4234)
                     /locus_tag="VGT87_001021"
     CDS             complement(3248..4234)
                     /locus_tag="VGT87_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEQ3813886.1"
                     /translation="MLEDYYPSTTSYYHGGIDDDLYTAKWGMVMTFLDLNDSSLTPFE
                     GTHFALIGFKSDKGVYINNGRVGAVESPAAIRTQLAKFPWHLGNQVMVYDVGNIDGPN
                     RSLEQLQNSLSKAIKRMCDLNLKPIVLGGGHETAYGHYLGLRQSLSPSDDLAVINMDA
                     HFDLRPYDQTGPNSGTGFRQMFDDAVADKRLFKYFVLGIQEHNNNLFLFDFVAKSKGI
                     QFLTGQDIYQMGHQKVCRAIDRFLEGQERVYLTIDMDCFSVGAAPGVSAIQSLGIDPN
                     LAVLVLQHIAASRKLVGFDVVEVSPPHDIDNHTANLAATFIFYLVQIMAQHS"
     gene            complement(4421..5962)
                     /gene="hutH"
                     /locus_tag="VGT87_001022"
     CDS             complement(4421..5962)
                     /gene="hutH"
                     /locus_tag="VGT87_001022"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEQ3813887.1"
                     /translation="MTRVINLDGESLTLEDVIAIARQGVACRIDDSAIEAVNASRKIV
                     DDIVSEKRVVYGVTTGFGSLCNVSISPEDTVQLQENLIRTHASGFGDPLPEDAVRAIM
                     LIRINSLVKGYSGIRLSTIEKLLELLNKGVHPYIPEKGSLGASGDLAPLAHMVLPMLG
                     LGKAYYKGELLSGQEALDKAGIDKISLAAKEGLALINGTTVLTAIGALATYDAIQLLK
                     LSDLAGALSLEVHNGITSPFEENLHTIRPQSGQLATARNIRNLLEGSQNTTVATQSRV
                     QDPYTLRCMPQIHGASKDSIAYVKSKVDIEINSVTDNPIICKDGHVISGGNFHGEPMA
                     QPFDFLGIAISEIGNVSERRVERLVNSQLSKLPSFLVKYPGLNSGFMITQYACASLAS
                     ENKVLAHPASVDSIPSCENQEDFVSMGTTAARKAFEILKNSRRIVATEIMAACQALDL
                     KPENHELGKGTKVAYDLFRKEVNFIEHDKHIEIYDELNKASTVIEDPSFLEAVEQAVE
                     LSIQF"
     gene            complement(5974..7317)
                     /locus_tag="VGT87_001023"
     CDS             complement(5974..7317)
                     /locus_tag="VGT87_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEQ3813888.1"
                     /translation="MASNHMNEQEREQAKFSLSGATLYGINAVIGSGIFLLPRAIYKG
                     LGPASIAVMFGTAILTIMLAVCFAEVSGYFGKNGGAFQYSKRAFGDFIGFNVGFLGWT
                     VTIFAWAAMAAGFARMFIITFPAFEGWHIPLSIGLIILLSLMNIAGLKTSKIVTITAT
                     IAKLIPIVAFCACTLFFIKNGLPNFTPFVQLEPGTNLLGAISNTAVYIFYGFIGFETL
                     SIVAGEMRDPEKNVPRALLGSISIVSVLYMLIIGGTIAMLGSQIMMTNAPVQDAFVKM
                     IGPAGAWMVSIGALISITGLNMGESIMVPRYGAAIADEGLLPATIAKQNQNGAPLVAI
                     LVSGAIAIVLLLTGSFENLAKLSVVFRFFQYIPTALAVMKLRKDAPDANVIFRVPFGP
                     IIPILAVIVSLVMIWGDNPMNFVYGAVGVIIASSVYYLMHGRNHQMDQMPPEEQI"
     gene            complement(7537..8133)
                     /locus_tag="VGT87_001024"
     CDS             complement(7537..8133)
                     /locus_tag="VGT87_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HEQ3813889.1"
                     /translation="MTKVTIKAPSDYLQTDWSGGETNQLFLYPEDGDYKKREFSYRLS
                     KSTVALRQTTFTLLDGYHRILMTLDKPITLSDLSHAKEISLTPFEPYAFEGDTRIVSR
                     GICQDMNLMYDDQYQGQLLPVWSDTLFEAPKADCQLLYALSKLTYSVTNQANQELEAN
                     HLLVIEQEASLSDLKVCLNQTSPAQTPVAVWAGLWYKT"
     gene            complement(8155..9828)
                     /locus_tag="VGT87_001025"
     CDS             complement(8155..9828)
                     /locus_tag="VGT87_001025"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEQ3813890.1"
                     /translation="MVLSDIEIANSVTMEPISKVADQLGIDKEALCLYGKYKAKIDAR
                     QLVALKGKPDGKLILVTAISPTPAGEGKTTTSVGLVDALSAIGEKAVIALREPSLGPV
                     FGVKGGAAGGGHAQVVPMEDINLHFTGDFHAIGVANNLLAALIDNHIHHGNSLGIDSR
                     RITWKRVVDMNDRQLRHIVDGLQGKVNGVPREDGYDITVASEIMAILCLSENISDLKA
                     RLEKIIIGYNYQGEPVTAKDLKAGGALAALLKDAIHPNLVQTLEHTPALIHGGPFANI
                     AHGCNSVLATKLALKYGDYAVTEAGFGADLGAEKFIDIKCRMSGLRPAAVVLVATIRA
                     LKMHGGVPKADLATENVQAVVDGLPNLDKHLANIQDVYGLPVVVAINKFPLDTDAELQ
                     AVYDACDKRGVDVVISDVWANGGAGGRELAEKVVTLAEQDNQFRFVYEEDDSIETKLT
                     KIVTKVYGGKGITLSPAAKRELADLERLGFGNYPICMAKTQYSFSDDAKKLGAPTDFT
                     VTISNLKVSAGAGFIVALTGTIMTMPGLPKVPASETIDIDEEGNITGLF"
     gene            complement(9846..10472)
                     /locus_tag="VGT87_001026"
     CDS             complement(9846..10472)
                     /locus_tag="VGT87_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HEQ3813891.1"
                     /translation="MGLVDLSLTDFAKVLGSDAPAPGGGSAAALSGANGISLTKMVCE
                     LTLGKKKYADYQVIIEDLHTKSSRLQQDLLDAIDKDTEAFNLVSAVFDMPKETDADKA
                     ARRTGMQKALKTAAQSPFEMMTLMVEALEITATAVGKSNTNAASDLGVAALNLKAGLQ
                     GAWLNVLINLSGIKDEDFVTDYRQKGQALLDKGCHLADDIYTKILDIV"
     gene            complement(10483..11382)
                     /gene="ftcD"
                     /locus_tag="VGT87_001027"
     CDS             complement(10483..11382)
                     /gene="ftcD"
                     /locus_tag="VGT87_001027"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003005485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEQ3813892.1"
                     /translation="MAKIVECIPNFSEGQNQAVIDGLVATAKSIPGVTLLDYSSDASH
                     NRSVFTLVGDDQSIQEAAFQLVKYASENIDMTKHHGEHPRMGATDVCPFVPIKDITTQ
                     ECVEISKQVAERINRELGIPIFLYEDSATRPERQNLAKVRKGQFEGMPEKLLEEDWAP
                     DYGDRKIHPTAGVTAVGARMPLVAFNVNLDTDNIDIAHKIAKIIRGSGGGYKYCKAIG
                     VMLEDRHIAQVSMNMVNFEKCSLYRTFETIKFEARRYGVNVIGSEIIGLAPAKALIDV
                     AEYYLQVEDFDYHKQVLENHLLG"
     gene            complement(11470..13500)
                     /locus_tag="VGT87_001028"
     CDS             complement(11470..13500)
                     /locus_tag="VGT87_001028"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002914331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEQ3813893.1"
                     /translation="MSFYSETDIAAAMTVKLDDVLPEKTVFKEGIRRAPDRGFRLTQA
                     QTEIALKNALRYVPTKFHEEVIPEFLEELKTRGRIYGYRFRPKDRIYGKPIDEYKGNC
                     TAAKAMQVMIDNNLSFEIALYPYELVTYGETGSVCANWMQYCLIKKYLEVMTDEQTLV
                     VESGHPVGLFQSKPEAPRVIITNGLLVGEYDNMKDWEIAEEMGVTNYGQMTAGGWMYI
                     GPQGIVHGTFNTLLNAGRLKLGVADDGDLTGKLFISSGLGGMSGAQGKAAEIAKAVAI
                     IAEVDQSRIETRHSQGWVSQIAESPEEALQLAQKAIDAKESTSIAYHGNIVDLLEYVN
                     DKQIHVDLLSDQTSCHNVYDGGYCPVGISFDERTRLLAEDKDTFHQMVDDTLARHFEA
                     IKTLTENGTYFFDYGNAFMKSVYDSGITEISKNGRNDKDGFIWPSYVEDIMGPMLFDY
                     GYGPFRWVCLSGKHEDLVTTDKAAMEAIDPDRRYQDRDNYNWIRDAEKNQLVVGTQAR
                     ILYQDCIGRVTIALKFNELVRKGKIGPVMIGRDHHDVSGTDSPFRETSNIKDGSNVTC
                     DMAVQCYAGNAARGMSLVALHNGGGTGIGKAINGGFGLVLDGSERIDEIIKSAIAWDT
                     MGGVARRNWARNEHAIETAIEYNRLHAGTDHITIPYLADDDLVTSAVNQLFQ"
     gene            13790..>14997
                     /locus_tag="VGT87_001029"
     CDS             13790..>14997
                     /locus_tag="VGT87_001029"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEQ3813894.1"
                     /translation="MVADVLLTHFNQLFCLNDPGHPLTGQEMKKATIVEDGYIAIKDG
                     LIVALGSGEPDAELVGPQTIMRSYKGKIATPGIIDCHTHLVYGGSREHEFAKKLAGVS
                     YLDILAQGGGILSTVRATRSASFDNLYQKSKRLLDYMLLHGVTTVEAKSGYGLDWETE
                     KRQLDVVAALEKDHPIDLVSTFMAAHAIPEEYKGDPKAYLDVIIKDMLPVVKEKNLAE
                     FCDIFCEKNVFTADESRYLLSKAKEMGFKLRIHADEIASIGGVDVAAELSAVSAEHLM
                     MITNDGIAKLIGAGVIGNLLPATTFSLMEDTYAPARKMIDAGMAITLSTDSNPGSCPT
                     ANMQFVMQLGCFMLRLTPIEVLNAVTINAAYSVNRQERVGSLTVGKEADIAIFDAPNI
                     DYLFYFFATN"
BASE COUNT         4183 a         3119 c         3401 g         4294 t
ORIGIN      
        1 atgaattccc ttattttcga gatgactagc ttctttgttt attggtaagt gttaagacaa
       61 gcttttgctc attttgggtg acacttgcca aatcgtttta tttaagatat agtgaacttg
      121 actaattttt ggaggcgttt tcatgacgaa gggtattcga tttcaattgc ttggcagtcc
      181 ccacattttt cttgatgaca aagaacaatt ttttgccttt gccaaagcaa atgctctcct
      241 ttattactta gtggttaatg gatctgttag ccgcgaggtg gcggcgagtc tactttggga
      301 aaataagaac acgcaaacag cgaaaaagaa tttacgaaac gctatctacc aggtcaataa
      361 agtcttgcaa gctgatgtga ttatttgtcc caatcgcaac ctgctggttt tgaataaaac
      421 acttgatatc aaaacagata ttaacctttt tctggctgat ccgctagctc acttagaact
      481 ctaccaaggg gagtttttgc agggctttta ccttaaaagt ggtgaggaat ttgatctttg
      541 ggtttcaaaa atgcgtatgc agtatgaaca ggtataccta aaagcgtgtt accaaaaaat
      601 tgaagaaaag ctctctttag atgccattga agatgtcgag gaacatttaa aacagcttat
      661 tgaacgtgat gaatttgaag agaaaaatta tcagctcttg atgaggcttt atcagcaggg
      721 gaattgtcct ggtaaggtca tcgaaactta ttatcagttg gctaatgtgt tggataagga
      781 gctagggatt cagccaagtt tgcaaagcca acagatttat caggaagtgg tcgctaaaga
      841 tcgtaatgaa cgcaaaatca agcatttttt gcgcaacagt aatcattttt tggggcggat
      901 tgatgagatt aagcagctgg aaaactattt tgccaactgt ttggcctgcc aagaggtagg
      961 agctctgctc ttaattggtg atacagggat tgggaaacga accctagcca gacaggtctt
     1021 agccaatcaa acccaaacgt ttcaaattgt cacagctaaa tgttttcgtg aagaggctat
     1081 ggattcctta ttgccttggc gtaatatctt agatggcctg ggagatttgg tgattcaaaa
     1141 ccgcttattg accaccaaag cttggaaggc tgctcttaaa cgctgttttc ctgcggcaac
     1201 catttttcaa gaagataaca accaaccctt tatcaaggac cacaccagct tgttggtttc
     1261 ctttattgtt gatattttac aacatttggc agaaatcaag gcgctggtaa tccttattga
     1321 ggattgtcat tggatggatg aggacagttt gaccttgttg caacgggtca tgaatcaatt
     1381 ggtccactat ccgattgctt ttgtcttgac caagcatcta ggaacgactc ctgagctcgg
     1441 tctttgtttg aatgctttga tgagtcaggg gcgtttagaa agcatttgtt tggagccttt
     1501 taatcggcaa gaaagtttgg cttatattag cagtcaattg ggtagtcagc cagtgacgga
     1561 agaagaaatg gagcacctct atcaagccag ccaaggcaac cctttctttt tgtcagaata
     1621 cactcaggcc ctactgcgtc acgaaaaatt tgtgccttta acgcctgcca ttaaggctaa
     1681 gttgggtctt aaactagcta atctaagtag tcgtgatgac gcattgttaa actatttgtc
     1741 ttgttgtcgg aggcctatcc ctctaaatac cctcgctcaa ttgatgttac tgcctttaga
     1801 agaagtgatc gagatggtgg ataatctggg gcactactat attttggtag aagaaagcgt
     1861 aggagaggag gtgttgattt catttcgtca gcgaattatt caactctata gttattaccg
     1921 tttatctttg tcaaaaaggc gtttattgca cggacaaatt gccaaacggt tagaagattt
     1981 actgcctatt ttgacaccaa gccctcactt attagatgac attgcttacc attatcagga
     2041 atcacggcag gtgattaagg ccttggaata caaccttaac tatctagatg ctaccttgcc
     2101 atttcagcat gagctgtttc ccatttattc aaagagtatt ggctccttgg aaaagtcaga
     2161 tcgtgaccat caacgcttaa tggaagagca gtttgataag attcggcaaa gcattgcgga
     2221 tttggaactg acctatgata ataaccgtga tttccagcag ttgcttattc gtttttctta
     2281 ccttgaaggc cgttatgata ttcgaacagg gaggtatcaa gaagggatta aacatattca
     2341 aaaggtcatc gctttggcaa ctgagctaaa acagccctcc ttcttgctag aaggctatcg
     2401 ccagttaatt cattactgta tccaagtgga aaacaagcca gaaatgcgtt attacactag
     2461 tctttcattg gaggcagcgg ttgctgctaa tcattttgag gccattgcga ttagtttgcg
     2521 gttaaacggt ctttatcatt tgattattgg ggagttaaat gaggcagagc gtctgcttca
     2581 acagtcgatt gacttcttta aggtgacgcc aggcttgcaa gctaactatg cgattcagat
     2641 tgctgctgcc ttagattatt tgggggagat tgcccagatt cgctatcagt ttgaaaaggc
     2701 cgttacttac caaaaacaag ccattgcctt gaccgaaaac aagccagctg agttatcggt
     2761 gagtattttt tatattggat tagggatttc ctacttttat ttagctgatt ttgaacaagc
     2821 tgagcaaatc ttgagtttgg ctaaggaagc tttggtcaat catagttatc cttggaaaga
     2881 aacgcaactt gaaatttacc tggccatgat tcaatggaaa aagggtaatt atcagccagc
     2941 cttgacccta cttgattaca gagaaacctt gatgtctagg tatcgcaatc cgcgtgatga
     3001 gggcttggtt ttctatttga tggctgtggt caagtaccaa ttgatatttc aaggagctac
     3061 cctaagtcag caggaaaagg aaatggcaga ccacctgttg tcagaatcct ttgagtatta
     3121 ttatgaaatt gctagtacta acctcaaccc ttaccgagat tgtcatttgg tgagtgagtt
     3181 gaacgatttg cgtcaacaat tatctgctaa aagctaagga aaaccctctg aagtgaatca
     3241 gagggtttta gctatgttgt gccataatct gtacgaggta aaagataaag gtggctgcta
     3301 aattggcggt gtggttatca atatcgtgtg gtggtgaaac ctcaaccaca tcaaagccaa
     3361 ctaattttct actagctgca atgtgttgca aaactaagac ggcaagattt gggtcaatac
     3421 cgagggactg gatagcacta accccaggtg cagcaccaac tgagaaacaa tccatgtcga
     3481 tagtcaggta gaccctttct tgcccttcta agaaacggtc aatggctcgg cagacctttt
     3541 gatgacccat ttggtagatg tcttgaccag tcaaaaattg aatgcccttg gattttgcca
     3601 caaagtcaaa taggaaaagg ttgttattat gttcttgaat gcctaaaacg aagtatttaa
     3661 agaggcgttt gtcagcaacc gcatcgtcaa acatttgtcg aaagcctgtc cctgaattag
     3721 ggcctgtttg gtcgtaaggc cttaaatcaa agtgggcgtc catattgatg acagctaggt
     3781 catcacttgg ggataggctt tggcgtaggc ctaagtagtg gccgtaggcg gtttcatggc
     3841 cacctcctaa gacgatgggt ttgagattga ggtcacacat ccgtttgatg gctttgctta
     3901 aactgttttg cagttgttcc aaagagcgat tggggccgtc aatgttaccc acatcgtaaa
     3961 ccataacttg gtttcctaaa tgccaaggga atttggcgag ctgggttcgt atagcagctg
     4021 gactttcgac cgctccgaca cgaccattat tgatataaac ccccttatca cttttaaagc
     4081 caatcaaggc aaaatgtgtc ccttcaaatg gggttagtga gctgtcgtta aggtcaagga
     4141 aagtcatgac catgccccat ttggcagtat ataagtcatc gtcgatccca ccatgataat
     4201 agctagtggt ggatgggtag taatcttcta gcatgcgcgt catctccttt aggattatga
     4261 tagcaaaata aaacttacag tcaactctct ataggctgtc atcactggtt tttaggaata
     4321 gcagagcaat aaaatcgtct gcttttggtc ccagttagtt agtctaaaga ggccatgacc
     4381 tctgccacag cccctctcag ttaaagaagt accttattca ctaaaattgg atactcaact
     4441 caactgcctg ctcaaccgct tcgaggaagc ttgggtcttc aatgactgtt gatgccttgt
     4501 ttagttcatc gtagatttcg atgtgtttat cgtgttcgat aaagttgact tccttgcgga
     4561 agagatcgta ggcaacctta gtccctttac ctagttcatg attttcaggt ttgagatcaa
     4621 gagcttggca ggctgccatg atttcagtag ccacgatgcg gcgagaattt ttaaggattt
     4681 caaaggcctt gcgagcagcg gtggtgccca tgctgacaaa gtcttcttgg ttctcacaag
     4741 atgggataga atccacactg gctggatgtg ccaaaacctt gttttcagaa gcaagagagg
     4801 cacaggcgta ctgggtaatc ataaagcctg aattgagacc tgggtatttg acaaggaagg
     4861 atggtagttt gctgagctgg ctattgacca aacgttccac acggcgttca gaaacatttc
     4921 caatttcaga aatggcaatg cctaagaagt caaatggctg tgccattggt tctccgtgga
     4981 agttacctcc tgaaataacg tggccatctt tacagataat tggattatct gtgacagaat
     5041 tgatttcaat gtcaactttg gatttgacat aggcgatact gtctttgcta gccccatgaa
     5101 tttgaggcat gcagcgcaag gtgtaagggt cttgcacacg agattgagta gctacggttg
     5161 tgttttggct gccttcaagg aggtttcgga tattgcgggc tgttgccaac tggccgcttt
     5221 gtgggcgaat ggtgtgaagg ttttcttcaa atggactagt aatcccgtta tgcacttcaa
     5281 gggatagggc accagctagg tctgacagtt tcaataattg aatggcgtca taggttgcaa
     5341 gggcaccgat tgcagtgaga acagttgttc cgttgataag ggcgagtcct tctttagcgg
     5401 ctaaagaaat cttgtcaata ccagctttat caagagcttc ttgaccagaa agaagctctc
     5461 ctttgtagta agctttccct agaccaagca taggaagcac catgtgagca agcggcgcca
     5521 aatctcctga agcccctaga gacccttttt caggaatgta aggatgaacc cctttattga
     5581 gcagttccaa cagcttttca atggtggata gacgaatacc agaataccct ttgacgagag
     5641 agttgatacg gatcagcatg atagctctca cggcatcttc tggcaatggg tcaccaaatc
     5701 cactagcatg ggtacgaatc aaattttctt ggagttggac tgtatcttct ggtgagatgc
     5761 tcacgttaca aagggatcca aaaccggtgg tcaccccgta aaccacccgt ttttcactga
     5821 caatgtcatc aacaatttta cgagaggcat tcacagcttc aatagcacta tcatcaatgc
     5881 gacaggctac gccttggcga gcaatcgcaa tcacgtcttc gagggtaagg ctttcgccat
     5941 ctaaattaat cactcttgtc atagtttttt ctcctaaatt tgttcttcag ggggcatttg
     6001 gtccatttgg tggttacgtc cgtgcataag gtagtacaca ctacttgcaa tgatgacacc
     6061 aactgcacca tagacaaagt tcattgggtt gtcaccccaa atcataacta aactaacgat
     6121 aactgcaaga atcgggataa tagggccaaa tggtactcgg aagatcacat tagcatcagg
     6181 agcatctttc cgtaatttca taacggctag tgctgttgga atgtattgga agaagcggaa
     6241 aacaacactg agttttgcta agttttcaaa tgagccagtg agtaggagta caatagcaat
     6301 tgctcctgaa accaagatag caacgagtgg cgcgccattt tgattttgct tggcaatagt
     6361 agctggtaag agcccttcat cagcaatggc agcaccgtaa cgtggcacca tgatggattc
     6421 tcccatgtta agacctgtaa tggaaattaa agcaccgatt gagaccatcc aagcgccagc
     6481 agggcctatc attttaacaa aggcatcttg aacaggtgca ttggtcatca tgatttgaga
     6541 gccaagcatg gcaatggttc caccgataat cagcatatat aagacagaga cgatactgat
     6601 tgatcctaaa agagcacgcg gtacattttt ttcagggtca cgcatttccc cagcgacaat
     6661 agagagggtt tcaaaaccga taaagccata aaagatataa acagcagtat tggagatagc
     6721 ccctaagagg ttagtccctg gttccaactg aacaaatggg gtaaagttag gaagcccgtt
     6781 tttgatgaag aaaagggtac aggcacaaaa ggcaacgatt ggaattaact tagcaatggt
     6841 ggctgtgatg gtgacaattt tagaggtttt caaaccagcg atattcatca aagatagtaa
     6901 gatgattaac ccaatgctaa gtggaatgtg ccaaccttca aaggcaggga aagtaatgat
     6961 aaacattctg gcaaacccag ctgccatggc tgcccaagcg aagatggtaa cggtccaacc
     7021 aaggaagcca acgttaaagc caataaaatc accaaaagct cgtttggaat attggaaggc
     7081 tccgccgttt ttcccaaaat aaccagagac ttctgcaaaa caaactgcca acatgatggt
     7141 caaaatagct gttccaaaca taacagcgat ggatgcaggg ccaaggccct tgtaaatggc
     7201 cctaggtaag agaaaaatac cagagccgat aacggcattg atcccgtaaa gagtggcacc
     7261 gctcaagcta aactttgcct gttctctttc ttgttcgttc atgtgatttg acgccataat
     7321 aatgctctcc tttttgtttg caaattgcgt tcaaatataa tggataaggt cgctaggaaa
     7381 tgaggagata gtcggtttag gagggtcctt cttgcaccta acgatagtga tgagctattg
     7441 gttagcgatg agaaataggt agccttttgt gatgatgtgt agtgatttca ttggagaaga
     7501 ataggcatga gtagtgtgat gcctatcttt atcccactaa gtcttatacc ataagcctgc
     7561 ccaaacagct actggagttt gggcggggct tgtttgattg aggcaaactt ttagatctga
     7621 aaggctggcc tcctgctcga ttaccaacaa gtgattggct tcaagctctt gattcgcctg
     7681 attggtcaca gaataagtca gtttgcttag ggcataaagc agttgacagt ccgctttagg
     7741 tgcctcaaat agtgtgtcag accagacggg taataattgc ccttggtatt ggtcatcata
     7801 cattaaattc atgtcttggc agattccacg actgacgatt ctggtatcgc cttcaaacgc
     7861 ataaggttca aaaggagtta gggaaatctc tttagcatga ctcaaatcac tcaatgtgat
     7921 gggtttgtct agggtcatta gaatcctgtg atagccatca agtaaggtaa aggttgtctg
     7981 tcgtagggcc acagttgact ttgagaggcg ataagagaat tctctctttt tgtaatctcc
     8041 atcttcggga taaaggaaca attggttggt ttcaccgcca gaccagtcag tttggaggta
     8101 atcagagggc gctttgatag ttacctttgt catttgttct ctccttgttt ccaattagaa
     8161 tagtcctgtg atgttgccct cctcgtcaat atcaatggtt tcgctggcag gtacttttgg
     8221 aaggccaggc atggtcatga tagtacccgt cagagcaacg ataaatcctg ctcctgcaga
     8281 cactttgagg ttgctaatag tcactgtaaa gtcagttggt gcgccaagtt ttttggcatc
     8341 gtctgagaag gaatattggg ttttggccat gcaaattggg tagttgccaa agccgaggcg
     8401 ttctaagtca gccagttcac gtttggctgc tgggctcaag gtgatgcctt taccaccgta
     8461 aactttggta acgattttag tcagtttggt ttcaatgcta tcgtcttcct cataaacaaa
     8521 gcggaattga ttgtcttgct cggctagtgt cacgactttt tcagctaatt cacggccacc
     8581 agctccgcca tttgcccaaa catcagaaat cacaacgtca acgccacgtt tgtcgcaggc
     8641 atcatagaca gcttgtaatt cagcatcggt atcaagcggg aatttattga tcgccacgac
     8701 aactggaagg ccatagacgt cttggatatt agccaaatgt ttgtcaaggt taggtaaacc
     8761 atccacaacg gcctgaacat tttcagtagc caggtctgct tttggcacac ccccatgcat
     8821 tttaagggca cgaatagtgg ctactaggac aacggctgct ggacgaaggc cggacatgcg
     8881 gcatttgatg tcaatgaatt tttcagctcc aaggtcagca ccaaatccag cttcggtaac
     8941 ggcataatca ccatatttca aggctagttt ggtggctagg acactattac agccatgggc
     9001 gatattagca aatgggccac cgtgaatgag ggctggtgtg tgttccaggg tttgcacgag
     9061 attaggatga atcgcatcct tgagcagtgc ggctagggca ccaccagctt tcaagtcttt
     9121 agctgttaca ggttcgcctt gatagttgta accgatgatg attttttcaa ggcgggcttt
     9181 gaggtcagaa atattttcgg ataggcacag gattgccatg atttcagagg cgacggtaat
     9241 gtcgtaacca tcctcacgag ggaccccgtt gaccttgcct tgcaagccgt caacaatatg
     9301 acgaagctgg cggtcattca tatccaccac acgtttccaa gtaatacgtc tggaatcaat
     9361 gcctaggcta ttgccatggt gaatgtggtt atcaatcaag gcagctaata agttgttggc
     9421 gacaccgatg gcatgaaagt cccctgtaaa gtgaaggttg atgtcttcca ttgggacaac
     9481 ttgcgcgtgg ccaccgccag cagctcctcc cttaacccca aaaacaggac caagagaagg
     9541 ttcacgaagg gcaataactg ctttttctcc aatggcagaa agagcatcta ccaatccaac
     9601 agaagtggtg gttttgccct caccagctgg tgttggtgag atagcagtga caagaatgag
     9661 tttgccatca ggtttgcctt ttaaggcgac cagttgacgg gcatcaattt ttgccttgta
     9721 cttaccatag agacagagcg cttccttgtc aatccctaat tgatcggcaa ccttactgat
     9781 aggttccata gtgacagagt tggctatttc aatatctgat aaaaccatag ttgctccttt
     9841 acttgttaaa caatatcgag aattttcgtg tagatgtcat ctgccaaatg acagcccttg
     9901 tctaataagg cttgtccttt ttggcgatag tcggtaacaa agtcttcatc tttgatgcca
     9961 gacagattaa tcaaaacatt caaccaagcg ccttgtaaac ctgcttttaa gtttaaagca
    10021 gcaactccta agtcgctagc agcatttgta ttagacttac cgacagctgt tgcagtgatc
    10081 tcaagtgctt cgaccatgag ggtcatcatc tcaaaaggtg attgtgcagc tgttttcaag
    10141 gccttttgca tgccagttcg tctggcagcc ttgtctgcgt cagtctcttt tggcatatca
    10201 aaaacggcag aaaccaggtt gaaggcttcg gtatctttgt caatggcatc taataagtcc
    10261 tgttgcaagc gactactttt ggtatgaagg tcttcaatga taacttgata atcagcatac
    10321 tttttcttac caagagtcaa ttcacagacc atcttggtta aggaaatgcc attagcgcca
    10381 gagagagcag cagcagaacc accacctgga gcaggggcat ctgagccaag gactttagcg
    10441 aaatctgtta agcttaaatc aactaaaccc ataagacacc ccctagccta ataaatgatt
    10501 ttctaacact tgtttatggt agtcaaagtc ctcaacttgc aagtagtatt cagccacatc
    10561 gattaaggct ttggctggcg ctaagccgat gatttcagaa ccaataacat tcacaccgta
    10621 gcgacgcgct tcaaatttga tggtttcaaa agtacggtaa agagaacatt tttcaaagtt
    10681 aaccatgttc atggagactt gggcaatatg acggtcttct aacatgacac cgatggcttt
    10741 acaatatttg tagccaccac ctgaaccgcg gataatttta gcaattttat gagcaatgtc
    10801 gatattgtca gtatccaaat taacattgaa agcaaccaat ggcatgcgag caccaacggc
    10861 agttacccca gcagttgggt gaatcttacg atctccgtaa tcaggtgccc aatcctcttc
    10921 caacagtttt tctggcatgc cttcgaattg tcctttacgg actttggcca ggttttgacg
    10981 ttctggacgt gtggcagaat cttcataaag gaagattggg attccaagtt cacggttaat
    11041 ccgttctgcg acttgtttgg aaatttcgac acattcttgt gtagtgatgt ctttgatcgg
    11101 gacgaacggg cagacatcgg ttgctcccat acgagggtgt tcaccatgat gtttggtcat
    11161 atcaatgttt tcagaagcgt atttcaccag ttggaaagct gcttcttgaa tagactggtc
    11221 atctccaact aaggtaaaca cgctccggtt gtggctcgca tcagaagagt agtcgaggag
    11281 ggtcactcca ggaatacttt tggccgtcgc taccaagccg tcgataacag cctgattttg
    11341 gccttctgag aagttgggaa tacattcaac aattttcgcc atgttaatct ccttttatgt
    11401 cattgagtta ctcattaagg gaatgggtta cctattatag ataacgctta catttttatc
    11461 tagctgcttt tactgaaata attgatttac cgctgaagtc acaaggtcat cgtcagccaa
    11521 atatggaata gtgatgtgat cagtacctgc atggaggcgg ttgtattcaa tagcggtttc
    11581 aatagcgtgc tcattgcgag cccaattgcg acgagcaaca ccacccatgg tgtcccaagc
    11641 aatagcagat ttaataatct catcaatgcg ttcgctacca tctagaacaa gtccaaagcc
    11701 gccgttaatg gctttaccga taccagttcc gccaccgtta tgaagagcca ctaaactcat
    11761 accacgcgca gcgttaccag cgtaacattg aactgccata tcacaggtga cattggaacc
    11821 gtctttaatg tttgaggttt cacgaaatgg tgagtcggta ccagacacgt catggtggtc
    11881 acgaccaatc atgacaggac caatcttgcc tttgcggacc aattcattaa atttaagggc
    11941 gatagtcaca cgaccgatac agtcttggta aaggatccgg gcttgggtac caactacgag
    12001 ttgatttttc tcagcatcac gaatccagtt gtagttatca cggtcttgat agcggcggtc
    12061 aggatcgata gcttccatgg cagctttgtc agtagtcacc aagtcttcgt gtttaccact
    12121 caagcagacc caacggaaag gtccatagcc atagtcaaaa agcataggtc ccatgatatc
    12181 ttccacatag gatggccaga taaagccgtc tttgtcgtta cgaccattct tagaaatttc
    12241 tgtaatacca gagtcgtaaa cagacttcat aaaggcattg ccgtagtcaa agaaataggt
    12301 gccattttca gttaacgtct taatagcttc aaaatgccgt gccaaggtat catcaaccat
    12361 ttggtggaag gtatccttgt cttctgccag gagccgggtt ctttcgtcaa agctaatacc
    12421 tactgggcag tagccaccgt cataaacgtt gtgacaagag gtttggtcag acaaaagatc
    12481 aacatgaatc tgcttgtcat tgacgtattc taaaaggtca acaatattac catggtaagc
    12541 aatcgaagtg gactccttag catctatggc tttttgggct aattgcagag cttcttcagg
    12601 gctctcagca atttggctaa cccagccttg cgaatgacgg gtttcaatcc gagattggtc
    12661 aacttcagca ataatggcaa ctgctttagc aatttcagca gccttacctt gtgccccact
    12721 catgcctcct aaaccagatg agataaagag tttaccggtc aaatcaccat cgtcagccac
    12781 tccaagtttg agacgaccag catttaagag agtattaaat gttccatgaa caatcccttg
    12841 tgggccaata tacatccaac cacctgcggt catttgacca tagttggtga cacccatctc
    12901 ttcagcgatt tcccagtctt tcatgttgtc gtattcacca accaaaaggc cattagtaat
    12961 aatcacacga ggggcttcag gtttggattg gaaaagacca acgggatgac cagattcaac
    13021 caccaaggtt tgttcatcag tcatgacctc taggtatttt ttaatcaaac aatattgcat
    13081 ccagttggca caaacagaac cagtttctcc ataagtgact agttcgtaag ggtacaaggc
    13141 aatctcaaaa ctcaagttat tgtcaatcat gacctgcatg gccttggcag ctgtacaatt
    13201 tcccttgtac tcgtcaatgg gtttgccgta aatgcggtct tttggtctaa agcggtagcc
    13261 ataaatacgg ccacgggttt tgagttcttc caaaaattct ggaatgactt cttcgtggaa
    13321 ctttgtaggt acatagcgta gggcattttt taaggcaatt tcggtctgag cctgggtcaa
    13381 tcgaaaacct ctgtctggtg ctcttcggat accttctttg aacacagtct tttccggtaa
    13441 aacatcgtct aacttaacag tcatagcggc agctatgtca gtttcactgt aaaatgacat
    13501 agaaaactcc tcctcaactc ctttttattg tcgagattag tctaacttag cctagtctat
    13561 aaaaagtcaa ccacgctttg aaatgcgaaa aaagtctaaa aaaaatcaaa acctcatttt
    13621 ttgactaaaa tttgacgctt aatcagtaca cttgaggtat gacagataaa gtcagtcaga
    13681 ggttaattgt tgttaccaaa gttgcaaaga tcgcaactat tgagtgggtg aggggggcta
    13741 gtaatggcta ttaatcagtt gacaaacgat gttaaaaaag gaggttttta tggttgctga
    13801 tgtcttatta acacatttta atcaactatt ttgtctaaat gatccaggtc atcctttaac
    13861 tggtcaagaa atgaaaaaag ccactatcgt tgaagatggc tatatagcca tcaaagacgg
    13921 cctcattgtg gctcttggtt ctggtgaacc agatgctgag cttgtcggtc cacagactat
    13981 tatgcgttct tataaaggta aaattgcaac gcctggtatt attgactgtc atacccattt
    14041 ggtctatggt ggcagtcggg aacatgaatt tgccaagaaa ttagcagggg tgtcttactt
    14101 ggatatttta gctcaaggtg gtggtatttt aagcacggtg agggccactc ggtcagccag
    14161 ctttgacaac ctttatcaaa aatccaagag attgctagac tacatgttgc ttcatggagt
    14221 gactaccgtc gaagcgaaaa gtggctacgg ccttgattgg gaaacagaaa aacgccaact
    14281 ggatgttgtt gctgctttag aaaaagacca tcctattgat ttggtgtcta cgtttatggc
    14341 agcccatgcg attcctgaag aatacaaggg ggatcctaag gcttatctcg atgtgattat
    14401 caaggatatg cttcctgttg ttaaagagaa aaatctggca gaattttgtg atattttctg
    14461 tgaaaaaaat gtttttacag ctgatgagtc ccgctatctg ttaagcaaag ccaaagaaat
    14521 gggcttcaag cttcgtattc acgcagatga aattgcgtct ataggcggag ttgatgtcgc
    14581 agcagagctc agcgcagtta gtgcagagca tttgatgatg ataacaaatg atggtatcgc
    14641 taagttgatt ggtgctggtg ttattggtaa tttgcttcca gccaccacct ttagtttaat
    14701 ggaagacact tatgcccctg ctcgcaaaat gatcgatgcc ggcatggcca tcaccctctc
    14761 aacagatagt aatccaggca gttgcccgac tgctaacatg cagtttgtga tgcaattagg
    14821 ttgttttatg ttacgtttaa caccgattga agtcttaaat gctgttacca ttaatgcggc
    14881 ctactctgtc aatcgtcagg agagagttgg tagtttgaca gttggtaaag aagcagatat
    14941 tgccatcttt gatgccccaa atattgatta cctattttat ttctttgcca ccaactt
//
LOCUS       DAUCLR010000039        14800 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.039, whole genome shotgun sequence.
ACCESSION   DAUCLR010000039 DAUCLR010000000
VERSION     DAUCLR010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14800
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.039"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..1105
                     /locus_tag="VGT87_001030"
     CDS             <1..1105
                     /locus_tag="VGT87_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEQ3813895.1"
                     /translation="IAKLEDFVQKNIVRASTTKRAQARRKQLEKIERLDKPTSARKSA
                     HMTFQADKPSGNVVLTVEKAAIGYNQHVLSEPINLDVHKLDAIAIVGPNGIGKSTLIK
                     SVIGQIPFIKGEVKYGANVETGYYDQTQSHLTSSNTVLEELWQDFSTTPEVDIRNRLG
                     AFLFSGDDVKKSVAMLSGGEKARLLLAKLSMENNNFLVLDEPTNHLDIDSKEVLENAL
                     IDFDGTLLFVSHDRYFINRLATKVLEITENGSTLYLGDYDYYLEKKAELEELARLAAG
                     ETVEETKEASATDYQLQKANQKERRRLTRRYEEIEARLETIEERIGAIQEDMHASNDT
                     AQLIAWQKEWDQLDQEQETLMEEWETIAEQIES"
     gene            1390..2358
                     /locus_tag="VGT87_001031"
     CDS             1390..2358
                     /locus_tag="VGT87_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEQ3813896.1"
                     /translation="MVTVSKEQHLDMFLKMERIREFDSRINKLVRRGFVQGMTHFSVG
                     EEAANVGAVAHLSYDDIIFSNHRGHGQSIAKDMDLNKMMAELAGKATGVSKGRGGSMH
                     LADFEKGNYGTNGIVGGGYALAVGAALTQQYKGTNNIAVAFSGDGATNEGSFHESVNM
                     AATWKLPVIFFIINNRYGISMSINNATNTPHLYTRAEAYGIPGFYCEDGNDVMAVYET
                     MGKAVEHVRGGNGPAIVEVESYRWFGHSTADAGKYRTKEEVDEWKEKDPMIKYRTYLT
                     SEGIATDDELDAIQAQVKKEVDDAYEFAQNSPDPELSVAFEDVWVD"
     gene            2417..3415
                     /locus_tag="VGT87_001032"
     CDS             2417..3415
                     /locus_tag="VGT87_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEQ3813897.1"
                     /translation="MSETKLMALREAVNLAMTEEMRKDENIFLMGEDVGVYGGDFGTS
                     VGMIEEFGPKRVKDTPISEAAISGAAIGAAITGLRPIVDVTFMDFLTIMMDAIVNNGA
                     KNNYMFGGGLITPVTFRVASGSGIGSAAQHSQSLEAWLTHIPGIKVVAPGNANDAKGL
                     LKSAIRDNNIVLFMEPKALYGKKEEVNQDPDFYIPLGKGDIKREGTDLTIVSYGRMLE
                     RVLQAAEEVAADGINVEVVDPRTLIPLDKELIIESVKKTGKLMLVNDAYKTGGFIGEI
                     ATMITESEAFDYLDHPIVRLASEDVPVPYARVLEQAILPDVEKIKAAIVKMANKGN"
     gene            3600..5009
                     /locus_tag="VGT87_001033"
     CDS             3600..5009
                     /locus_tag="VGT87_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEQ3813898.1"
                     /translation="MAFEIIMPKLGVDMQEGEIIEWKKQEGDTVNEGDILLEIMSDKT
                     NMELEAEDSGVLLKIVRPAGDTVLVTEVIGYIGAEGESVDTIASSEKTTEIPVPASAD
                     AGPAVAPKENVASPAPQVAATAIPQGNGGKVRATPAARKVAAEMGIDLGQVPGTGPKG
                     RVHKEDVENFKGAQPKASPLARKIAADKGIDLATVSGTGFNGKVMKEDIMAILEAAKP
                     AEAKAPAAKEEKVVDLPEGVEYKPMSAMRKAISKGMTNSYLTAPTFTLNYDIDMTEMI
                     ALRKKLIDPIMAKTGLKVSFTDLIGMAVVKTLMKLEHEYMNASLINDANDIELHRFVN
                     LGIAVGLDDGLIVPVIHGADKMSLSDFVLASKDVIKKAQTGKLKAAEMSGSTFSITNL
                     GMFGTKTFNPIINQPNSAILGVGATIPTPTVVDGEIVARPIMAMCLTIDHRLVDGMNG
                     AKFMVDLKKLMENPFELLI"
     gene            5340..7103
                     /gene="lpdA"
                     /locus_tag="VGT87_001034"
     CDS             5340..7103
                     /gene="lpdA"
                     /locus_tag="VGT87_001034"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEQ3813899.1"
                     /translation="MAVEIIMPKLGVDMQEGEIIEWKKQEGDTVNEGDILLEIMSDKT
                     NMELEAEDSGVLLKITRQAGETVPVTEVIGYIGAEGESVEVSSPAASDVNVARTTEDL
                     EAAGLEVPKAPAQAASAAPKAALADDEYDIIVVGGGPAGYYAAIRGAQLGGKIAIVEK
                     SEFGGTCLNVGCIPTKTYLKNAEILDGIKIAAGRGINLASTNYTIDMDKTVDFKNTVV
                     KTLTGGVQGLLKANKVTIFNGLGQVNPDKTVTIGSQTIKGRNVILATGSKVSRINIPG
                     IDSKLVLTSDDILDLREMPKSLAVMGGGVVGIELGLVWASYGVDVTVIEMADRIIPAM
                     DKEVSLELQKILSKKGMKIKTSVGVSEIVEANNQLTLKLNNGEEVVAEKALLSIGRVP
                     QMNGLENLNLEMDRNRIKVNDYQETSIPGIYAPGDVNGTKMLAHAAYRMGEVAAENAI
                     HGNTTRKANLKYTPAAVYTHPEVAMVGLTEEQAREQYGDVLIGKNSFTGNGRAIASNE
                     AHGFVKVIADAKYHEILGVHIIGPAAAEMINEAATIMESELTVDELLLSIHGHPTFSE
                     VMYEAFADVLGEAIHNPPKRK"
     gene            7414..7491
                     /locus_tag="VGT87_001035"
                     /pseudo
     CDS             7414..7491
                     /locus_tag="VGT87_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(7589..9466)
                     /locus_tag="VGT87_001036"
     CDS             complement(7589..9466)
                     /locus_tag="VGT87_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEQ3813900.1"
                     /translation="MSHFLVEKLTKTVGDKTVFQDISFIIHDFDRIGIIGVNGTGKTT
                     LLDVLSGRLGFDGDHSPFSKANDYKIAYLTQDPEFNDAASVLDTVLSADVKAIQLIRQ
                     YELLMANYTEDKQETLESLMSEMDRLDAWSIESDVKTVLSKLGITDLEQKVGDLSGGM
                     RRRVQLAQVLLGAADLLLLDEPTNHLDIDTIAWLTTYLKTAKKTVLFITHDRYFLDHV
                     ATRIFELDKAGLTEYQGNYQDYVRLKAEQDERDAANLHKKKQLYKQELAWMRTQPQAR
                     ATKQQARINRFTDLKKEVHQDSSADKLEMAFETSRIGKKVIHFEDLSFAYGDRQLIKD
                     FNLIIQNKDRIGIVGDNGVGKSTLLNIINGDLKPTSGKLDTGDTIRVGYFSQQLKDMD
                     ETKRVINYLQEVADEVKTSVGTTSISELLEQFLFPRSSHGTLIAKLSGGEKKRLYLLK
                     LLIEKPNVLLLDEPTNDLDIATLTVLENFLTNFAGPVITVSHDRYFLDKVATKILAFE
                     EGDIRVFYGNYSDYLDEKAFEKETVEADLAKAIVTEEVPLPPKEEKRRMSYLEKQEWA
                     QIEDKIATIEANIEEIENQMLTVVSDYDQLAQLQKELDQRNNDLLLAYERFEYLSGLD
                     I"
     gene            complement(9463..10671)
                     /locus_tag="VGT87_001037"
     CDS             complement(9463..10671)
                     /locus_tag="VGT87_001037"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEQ3813901.1"
                     /translation="MKLMTMPSEFQKALPILTKIKEAGYEAYFVGGSVRDVLLGRPIH
                     DVDIATSSYPEETKAIFNRTVDVGIEHGTVLVLENGGEYEITTFRTEDVYVDYRRPSQ
                     VSFVRSLEEDLKRRDFTVNALALDENGQVIDKFRGLIDLKQKRLRAVGKAEERFEEDA
                     LRIMRGFRFAASLDFDIEATTFEAMRSHSPLLEKISVERSFTEFDKLLMAPHWRKGIS
                     AMIACQAYDYLPGLKQQEAGLNHLIVSLKDNFTFSDYHQAWAYVMISLAIEDPKSFLK
                     AWKTSNDFQRYVTKLIALYRIRQERSFEKLDIYQYGKKMASLVEDLRKAQSLSVDMDR
                     INTLDQALVIHDKHDIVLNGSHLIKDFGMKPGPQLGLMLEKVELAIVEGRLDNDFTTI
                     EAFVREELAT"
     gene            complement(10796..11644)
                     /locus_tag="VGT87_001038"
     CDS             complement(10796..11644)
                     /locus_tag="VGT87_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEQ3813902.1"
                     /translation="MKLAVITDSTATLPTDLKQDKAIFSLDIPVIIDDETYFEGRNLS
                     IDDFYQKMADSQNLPKTSQPSLSELDNLLGLLSSKGYTHVIGLFLAGGISGFWQNIQF
                     LAEEHPEIEMAFPDSKITSAPLGSMVKNVLDWSRQGMTFQAILNKLQEQIDGTTAFIM
                     VDDLNHLVKGGRLSNGSALLGNLLSIKPILRFDEEGKIVVYEKVRTEKKAMKRLVEIL
                     NDLIADGQYNVSIIHSKAQDKADYLKRLLQDSGYQYDIEEVHFGAVIATHLGEGAIAF
                     GVTPRL"
     gene            complement(11644..12024)
                     /locus_tag="VGT87_001039"
     CDS             complement(11644..12024)
                     /locus_tag="VGT87_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEQ3813903.1"
                     /translation="MQLVREKEFVNQYHYDARNLEWEKENGTPETNFEVTFQLIDKDE
                     QQKETVIVSVLQFVIVKEEFVISGVISQMVRILDRLVDKPSEFTQEEVESLAAPLLDM
                     VKRLTYEVTEIALDRPGIHLEFKN"
     gene            complement(12271..12428)
                     /locus_tag="VGT87_001040"
                     /pseudo
     CDS             complement(12271..12428)
                     /locus_tag="VGT87_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051153.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
     gene            12927..13952
                     /locus_tag="VGT87_001041"
     CDS             12927..13952
                     /locus_tag="VGT87_001041"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922160.1"
                     /note="This family includes IgM or IgG-cleaving cysteine
                     proteases; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IdeS/Mac family cysteine endopeptidase"
                     /protein_id="HEQ3813904.1"
                     /translation="MRKRCYSTSAAVLAAVTLFALSVDRGVIADSFSANQEIRYSEVT
                     PYHVTSVWTKGVTPTANFTQGEDVFHAPYVANQGWYDITKTFNGKDDLLCGAATAGNM
                     LHWWLDQNNEKIEAYLKKHPDKQKIMFGDQELLDVRKVINTKGDQTNSELFNYFRDKA
                     FPGLSARRIGVMPDLVLDMFINGYYLNVYKTQTTDVNRTYQEKDRRGGIFDAVFTRGD
                     QSKLLTSRHDFKEKNLKEISDLIKKELTEGKALGLSHTYANVRINHVINLWGADFDSN
                     GNLKAIYVTDSDSNASIGMKKYFVGVNSAGKVAISAKEIKEDNIGAQVLGLFTLSTGQ
                     DSWNQTN"
     gene            13972..14337
                     /locus_tag="VGT87_001042"
     CDS             13972..14337
                     /locus_tag="VGT87_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813905.1"
                     /translation="MSSTDDGILRTNTIAGNFFIDGQQQKTLINPIKIDKDIVTIQEF
                     DFKIRKFLMESKEIHLTKSPYIRGSLEIHSKNRKHEKINLYDAKPNSTRSDVLKKYKD
                     NKTINMKDFSHFDIYLWTK"
     gene            14541..14800
                     /locus_tag="VGT87_001043"
                     /pseudo
     CDS             14541..14800
                     /locus_tag="VGT87_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT         4688 a         2874 c         2840 g         4398 t
ORIGIN      
        1 aattgccaag ttagaagatt ttgttcaaaa aaatattgtc agggcttcaa ctacaaaacg
       61 tgctcaggcg agacgtaagc aattggaaaa aatagaacgc ttagacaaac cgactagtgc
      121 tagaaaatca gctcacatga cttttcaggc agataagccg tctggcaatg tggtgttgac
      181 ggttgaaaaa gctgctattg gttataacca acatgtctta tctgaaccta ttaacctaga
      241 cgttcacaaa ttagatgcga ttgctattgt tggtccaaat ggtattggta aatccactct
      301 gattaaatct gttattggac agattccttt catcaaagga gaagtgaaat acggtgccaa
      361 tgttgaaaca ggttactatg accaaaccca atcacatttg acgtctagca acacagtttt
      421 ggaagaatta tggcaggatt tttcaaccac gccagaagtt gatatcagaa accgattagg
      481 agcctttctc ttttctggag atgatgtcaa aaaatcggtg gccatgctgt caggtggaga
      541 aaaagcccgt ctgcttcttg cgaagttatc tatggaaaac aataattttc ttgttctgga
      601 cgagccgact aaccacctag acattgacag taaagaagtg ctagagaatg ccctcattga
      661 ctttgacggc accttgcttt ttgtcagcca cgaccgctat ttcattaatc gtttggcaac
      721 taaggttctg gaaattaccg aaaatggctc aaccctttac ttaggtgact atgattatta
      781 ccttgagaaa aaggcagagc tagaagaact agcccgccta gcagcaggag agacagttga
      841 ggaaacaaaa gaagcctctg ccactgatta tcagctacaa aaagctaatc aaaaagagcg
      901 tcgtcgcttg acccgccgat atgaggagat tgaagcaaga cttgaaacca ttgaagagcg
      961 cattggtgct attcaagaag acatgcatgc ctccaatgat acggcgcagt taatcgcttg
     1021 gcaaaaggaa tgggaccaat tggatcaaga acaagaaact cttatggaag aatgggaaac
     1081 catcgctgaa caaatagaat cataaaaata agctttagca gttttgctaa gcttattttt
     1141 atcgctttgc tacttgtttg aaggtgtgaa agaggtttta aaatgatatg ttttttatgt
     1201 caaggtcggt aaaacgtgaa actgttatat agtattttta aattttttag tacagttttg
     1261 tttgtaattt cacaaactat tgcatttcta aaaaaccaaa taaggtaagg tagaaagcga
     1321 tttaattatt tgcaaattgt gaaaaaaagt tttatcataa aaaggtaata tgataatatg
     1381 gaggccgaaa tggtaacagt ttctaaagaa caacatttgg atatgttcct taaaatggaa
     1441 cgtattcgtg aatttgattc acgtattaat aaactcgtcc gtcgtggatt tgtacaaggg
     1501 atgactcact tctcagttgg tgaagaagca gccaatgttg gagcggtggc tcacttgtct
     1561 tacgatgaca ttattttttc aaatcaccgt ggacatggac aatcaatcgc taaagacatg
     1621 gaccttaata aaatgatggc tgagcttgct ggtaaagcaa ctggtgtgtc taaagggcgt
     1681 ggtggctcaa tgcacttggc tgattttgaa aaaggaaact atggtaccaa tggtatcgtt
     1741 ggtggtggtt acgctcttgc agttggtgct gccctcactc aacaatacaa aggaactaac
     1801 aatatagctg ttgctttctc aggtgatggc gctactaacg aaggatcatt ccacgagtct
     1861 gttaatatgg ctgcaacatg gaaacttcct gttattttct tcatcatcaa caaccgttat
     1921 ggaatttcaa tgagcatcaa taatgcaaca aatacaccac acttatacac acgtgcagaa
     1981 gcttacggaa ttcctggttt ctactgtgaa gatggcaatg acgtgatggc tgtttatgaa
     2041 acaatgggga aagccgttga acatgttcgt ggtggaaatg gtccagctat tgttgaggta
     2101 gaatcatacc gttggtttgg tcactcgact gcagatgctg gtaaataccg tactaaagaa
     2161 gaagtggatg agtggaaaga aaaagatcca atgatcaaat atcgtactta tctgacaagc
     2221 gaaggcattg ccactgatga cgaattggat gctattcaag cacaagtcaa aaaagaagtt
     2281 gacgatgctt atgaatttgc acaaaacagt ccagatccag aactttcagt agccttcgaa
     2341 gacgtttggg ttgactaaga tcaccttgtc agtaagaagc aatcgtattt gaataataaa
     2401 tggagaagta aaaatgatgt cagaaacaaa attaatggct ttgcgtgaag cagtcaacct
     2461 tgctatgact gaggaaatgc gcaaggacga aaacattttc cttatgggag aagacgtagg
     2521 ggtttacgga ggagacttcg ggacttctgt tggaatgatt gaagaatttg gtcctaaacg
     2581 tgttaaagat accccaattt cagaagctgc tatctctggt gcagctattg gtgcagcgat
     2641 tacaggacta cgtccaattg tcgacgttac cttcatggat ttccttacca tcatgatgga
     2701 cgctatcgtg aataatggtg ctaaaaataa ttacatgttc ggtggaggcc ttattacccc
     2761 tgtaaccttc cgtgtggcat caggttctgg tattggttcc gctgcgcaac actcacaatc
     2821 tcttgaagca tggttgactc acattccagg aatcaaagtt gttgctcctg gtaatgccaa
     2881 cgatgctaaa ggcttattaa aatcagctat ccgtgataat aacattgttc ttttcatgga
     2941 accaaaagcg ctttacggta aaaaagaaga agttaaccaa gatcctgact tctatatccc
     3001 acttggcaaa ggtgacatca aacgtgaagg aactgatttg acaattgtgt catacggtcg
     3061 tatgttggag cgtgttcttc aagcggctga agaagttgca gcggatggta ttaacgttga
     3121 agttgttgac ccacgtaccc tcattccact tgataaagag ttgattattg aatctgttaa
     3181 gaaaacaggt aaattgatgc ttgtcaacga tgcctacaag accggtggat tcattgggga
     3241 aattgcaacc atgattaccg aaagtgaagc ctttgattac cttgatcatc caatcgttcg
     3301 tcttgcaagt gaagatgttc cagtacctta tgcacgtgta cttgaacaag cgatcttacc
     3361 agacgttgag aaaatcaaag ctgccattgt gaaaatggca aacaaaggca actagaagat
     3421 aaggcaagtg gtctgataaa ggctatgttt atcagtaacg tctcagctaa aaactatttt
     3481 aaaagttttt agaagaactg agttgtctta tcccgtgtcg cggcacctca cctttcgtat
     3541 gttttcgtaa caaggtgggg atgttgatgg cgcttatcaa atcgtgaaag gatgtagaaa
     3601 tggcatttga aattattatg ccaaagctgg gcgttgacat gcaagaaggc gagatcatcg
     3661 agtggaaaaa acaagaaggt gataccgtca atgaaggtga tatccttctt gaaatcatgt
     3721 cagacaaaac caatatggaa cttgaggcag aagactcagg tgtgctattg aaaattgtcc
     3781 gtccagctgg tgatactgta cttgtaactg aagttatcgg ttacattggt gcagagggtg
     3841 aatcagtaga tactattgct tcaagtgaaa aaacaacaga aattccagtt cctgcttcag
     3901 cagatgctgg accagcagtt gctccaaaag aaaatgtggc aagtccagct cctcaagtgg
     3961 cagcgacagc tattccgcaa ggaaatggtg gaaaagttcg tgctactcca gctgcacgta
     4021 aggtagcagc agaaatgggc attgaccttg gccaagttcc aggaacagga ccaaaaggac
     4081 gtgtccataa agaagacgtt gaaaacttta aaggtgctca accgaaagct tcaccacttg
     4141 ctcgtaaaat tgcggcagac aaaggtattg atttggcaac cgtatcaggt actggcttta
     4201 acggtaaggt catgaaagaa gacatcatgg ctattcttga agcggctaaa ccagctgaag
     4261 caaaagcccc agcagctaaa gaagaaaaag ttgttgacct tccagaaggt gtggaataca
     4321 agccaatgtc agctatgcgt aaggcaatct ctaaaggtat gacaaactct taccttactg
     4381 cccctacctt tacgcttaac tacgatattg acatgacaga gatgattgcc cttcgtaaga
     4441 agttaattga cccaatcatg gctaaaacag gccttaaagt cagctttaca gacttgattg
     4501 gtatggcagt ggttaaaacc ttgatgaaac ttgagcatga gtacatgaat gcttccctca
     4561 tcaatgatgc taatgatatt gaattgcacc gctttgtgaa ccttggtatt gccgtaggtc
     4621 ttgatgatgg tcttatcgtt cctgttatcc atggggctga caagatgagc ttgtcagatt
     4681 ttgtgcttgc ttcaaaagat gtgattaaaa aagcgcaaac tggtaagttg aaggcagctg
     4741 aaatgtctgg atcaaccttc tctatcacaa acttgggaat gtttggtacg aagacattta
     4801 acccaattat taaccaacca aactcagcaa ttcttggtgt tggagcaact atcccaactc
     4861 caacggttgt ggatggtgag attgttgccc gtccaattat ggcgatgtgc ctaaccatcg
     4921 accaccgttt agttgatggt atgaacggtg caaaattcat ggttgatctc aagaaattga
     4981 tggaaaatcc atttgaattg ttaatctgat caatcagttt taaacaagaa aagcattcaa
     5041 taataaaagg gattttgcta agtttagact tggtgggtgc ccttttctca aaaatataga
     5101 aaggatgtag aacgaggaga tctcatgcaa atgagtgata agcaattgaa tccgagcgaa
     5161 aagctcggat aaaaatggta agagttggct aatcttagcg tcgtagacgt ctgaactttc
     5221 ctcaattttt gtttcggaaa gtcctagtcg tcctgacgtg ggtgcgtcaa cgaaatcaaa
     5281 gatttctgac ttaccgcaaa aattcaatcg ctttctcctt gctcttggta tcttaaatta
     5341 tggctgttga aattattatg ccaaaactcg gtgttgacat gcaagaaggt gaaatcatcg
     5401 agtggaaaaa acaagaaggt gatactgtca atgaaggcga tattcttctt gaaatcatgt
     5461 cagataaaac caatatggaa cttgaagcag aagactcagg tgttcttctt aaaattacac
     5521 gtcaagcagg tgaaacagta cctgtaacag aagttatcgg atatatcggt gctgaaggtg
     5581 aatctgttga ggtttctagc ccagctgctt cagatgtgaa tgttgctcgt acaacagaag
     5641 atttagaagc tgctggactt gaagtgccaa aagcaccagc tcaagctgct tcagctgcac
     5701 caaaagctgc acttgctgat gatgagtatg acatcatcgt tgttggtggt ggccctgctg
     5761 gttattatgc agctattcgt ggtgctcaac ttggtggtaa aattgccatc gttgagaaat
     5821 ctgaatttgg tggaacttgc ttgaacgtag ggtgtatccc aaccaaaact taccttaaaa
     5881 atgctgaaat ccttgatggt attaagattg cagcaggacg tgggattaac cttgcctcaa
     5941 ctaactatac cattgacatg gacaaaacag ttgactttaa aaacaccgtt gttaaaacct
     6001 taacaggcgg cgttcaaggt cttctaaaag ctaataaagt cactatcttt aatgggcttg
     6061 gtcaagttaa ccctgacaag actgtaacca ttgggtcaca aaccattaag ggtcgcaacg
     6121 ttatccttgc tacaggttct aaagtatcac gtatcaatat cccaggaatt gactctaaac
     6181 ttgtcttaac gtctgacgat atccttgacc tccgtgaaat gccaaaatca ctagcagtta
     6241 tgggcggtgg tgttgttggt atcgagcttg gacttgtttg ggcatcttac ggtgtggatg
     6301 ttaccgttat tgaaatggct gaccgcatta tcccagctat ggataaggaa gtctctcttg
     6361 aacttcaaaa aatcctttct aagaaaggca tgaagatcaa aacatctgtt ggtgtctctg
     6421 aaattgttga agcaaataac caattgacct tgaaacttaa caatggtgaa gaagttgtcg
     6481 ctgaaaaagc tcttctttct atcggtcgtg taccacaaat gaacggtctg gaaaatctta
     6541 accttgaaat ggatcgtaac cgtatcaaag ttaatgacta ccaagagaca tcaattccag
     6601 gtatctatgc gccaggtgac gttaacggaa cgaaaatgct tgctcacgct gcttaccgta
     6661 tgggtgaagt ggctgcagaa aatgcgatac atggcaacac aactcgtaaa gctaacctta
     6721 aatacactcc agcagccgtt tacacacacc ctgaagtggc aatggttggt ttaactgaag
     6781 aacaagcgcg tgaacaatat ggtgatgtgc ttattggtaa aaacagcttt actggtaatg
     6841 gacgtgcgat tgcttcaaat gaagcccatg gttttgttaa agttattgct gatgccaaat
     6901 accacgaaat cttaggtgtt catattattg gtccagctgc agctgagatg attaacgaag
     6961 cagctactat tatggaatct gaattaaccg ttgatgaatt gttattatca attcatggac
     7021 acccaacctt ctctgaagtg atgtacgaag cctttgcaga tgtgcttggc gaagctatcc
     7081 ataacccacc aaaacgtaag taaaacgtct ttgtcttatt aactaatgaa aaatatcatc
     7141 acataacaga tcacaaaaaa gactagcaca actatgaact gcaccccaaa cgttagatat
     7201 aaaaccctaa cgtttggggt gtttttgtat gacattaagc tatgaagata agctagatat
     7261 ttagcgctaa aaaaaaccga gggaactccg tttgaaagaa gcatctctgc aaagaagaag
     7321 acaaaaaacc attataaaga atggacaaag acgagctctg attagatttt cttttcagaa
     7381 cggctaaagt gactcgttct acctactatt atcatcgatt attgcaacaa taaacgtatc
     7441 aaaataaaac gaaaaggact tagtcctcta caacacagaa ctaaatcctt tacttcaaat
     7501 agagtagcca acttttgggg ctcagtgcaa gagcacttct tttctattat cacttgttta
     7561 ttataaacat aacattacca tagtatactt atatgtctag tccactcaaa tactcaaacc
     7621 gttcatacgc caataataaa tcattattgc gctgatcgag ttccttttgt aattgagcta
     7681 actggtcata atcactaact acagttaaca tttggttttc aatttcttca atattagcct
     7741 caatagttgc tattttatct tctatctgag cccattcttg tttttcaagg taggacatgc
     7801 gccttttctc ctctttcgga ggaagtggta cttcttcagt gactattgct ttagctaagt
     7861 cagcttcaac tgtttctttt tcaaaagctt tttcatcaag gtaatcactg taattgccat
     7921 aaaaaacacg aatatcgcct tcttcaaagg ctaaaatctt agtggctact ttgtctagga
     7981 aataacgatc gtgactaacc gtaatgactg gtcctgcaaa gttagttaaa aagttttcta
     8041 agaccgtcaa ggtggcaatg tctaaatcat ttgttggctc atcaaggaga agaacatttg
     8101 gcttttcaat gagcagtttt agcaagtaga gccgtttttt ctccccacca gataatttag
     8161 caatcagcgt gccatgactt gatctcggaa aaagaaattg ttctaaaagc tcactgatac
     8221 tcgttgttcc cacgcttgtt ttaacctcat cagccacttc ttgaaggtaa ttaatgaccc
     8281 gtttggtctc atccatatct ttgagctgct gagagaaata acctacacga atagtatctc
     8341 cagtatcaag cttcccagaa gttggtttga gatcaccgtt gatgatattt aacagggtag
     8401 attttccgac gccgttatct ccaacaattc caatacgatc tttattttga ataatcaagt
     8461 taaaatcctt aatcaactgt cggtcgccat aagcaaaaga aaggtcttca aagtgaatca
     8521 ccttcttccc aattcggctc gtttcgaaag ccatctccaa cttatccgca gaactgtctt
     8581 ggtgaacttc ttttttcaag tcagtgaaac gattgattct agcctgctgt ttggttgcac
     8641 gcgcctgtgg ctgcgtcctc atccaggcta attcttgttt atagagttgc ttcttcttgt
     8701 gtaaattagc agcatctcgt tcgtcttgtt ctgcttttaa gcgcacatag tcttggtaat
     8761 tgccttgata ttctgtcagt cccgctttat ccaattcaaa aatacgggta gccacatgat
     8821 caaggaaata acggtcatgg gtaataaaaa ggacggtttt tttagctgtc ttgagatagg
     8881 ttgttaacca agcgatggta tcaatatcga gatggttggt cggctcatct aataggagta
     8941 agtcagcagc tcctagcaat acttgagcta gctggacacg tcttctcata ccacctgata
     9001 ggtctcccac tttttgttcc aaatccgtga tgcctaattt tgataggact gttttgacat
     9061 cactttcaat agaccaagca tctaagcgat ccatttcaga catgagggac tctaaggtct
     9121 cttgtttatc ttcagtataa ttcgccatca atagttcata ctgacgaatc agctgaatcg
     9181 ccttaacatc agctgacaat accgtatcta aaacagaggc tgcatcgtta aattcaggat
     9241 cttgtgttaa gtaagcgatt ttgtaatcat tggctttaga aaatggtgaa tggtctccgt
     9301 caaaaccaag ccttcccgac aagacatcta aaagagttgt ttttcctgtc ccattaaccc
     9361 caataatccc aatacggtca aagtcatgaa taataaagga aatatcttga aagacggtct
     9421 tatctcccac cgtttttgtc aatttctcca ccaaaaaatg gctcatgttg ctaattcctc
     9481 tctaacaaat gcttcaattg tagtgaaatc attatctagg cgcccctcta caatcgctaa
     9541 ttcaactttc tcaagcataa gccctagctg aggacctggt ttcatcccaa aatccttaat
     9601 caggtgagaa ccattaagga cgatatcgtg tttgtcatga ataaccagtg cctgatctaa
     9661 agtattaata cgatccatgt caacggataa gctttgagct tttcggaggt cttccactag
     9721 actcgccatt ttcttgccgt actggtaaat atccaatttt tcaaaagatc tttcctgacg
     9781 aattctataa agagcaatca actttgtgac ataacgctga aaatcatttg aagtcttcca
     9841 tgcttttagg aagcttttag gatcctcaat ggctaaacta atcatcacat aagcccaagc
     9901 ttggtgataa tcactaaagg taaagttatc tttcaaagaa actatcagat gattcaagcc
     9961 tgcttcctgc tgcttcaagc ctggtaaata gtcgtaagcc tgacaagcaa tcatggcaga
    10021 aatccctttt cgccagtgcg gtgccatcaa taatttatca aattcagtaa aagaacgttc
    10081 cacagaaatt ttttccaata gaggtgaatg gctcctcata gcttcaaaag ttgtagcttc
    10141 aatgtcaaaa tccagactcg ccgcaaatcg aaagcctctc ataatgcgta gtgcatcttc
    10201 ttcaaaacgt tcctctgctt tacccactgc tcgtaaccgt ttttgcttta aatcaatgag
    10261 tcctctaaat ttatcaataa cttgaccatt ttcatccaag gccaaggcat tgacggtaaa
    10321 gtctcttcgt tttaagtctt cctctaaaga gcgcacaaaa gaaacctgac taggacggcg
    10381 ataatcaaca tagacatctt ctgtcctaaa ggttgtgatt tcatactcac cgccattttc
    10441 aagcacgagc actgtaccat gttcaatgcc aacatcgaca gttctgttaa aaatcgcttt
    10501 agtctcttca ggataggaac tagttgcaat atctacatca tgaatgggac gcccgagtaa
    10561 aacatctctg acgcttcctc ccacaaagta ggcttcataa ccagcttctt ttattttagt
    10621 caatatgggt aaagccttct gaaactcaga aggcattgtc attaatttca tacgttgttt
    10681 tctaacctat catccaacta gcccaatcta aagtgtgatt aggctacttg ggttctatca
    10741 cattttgtta ttgttttagt gattcttgca attaactatt ttttctaatc gacaattaaa
    10801 gcctcggtgt tacgccaaat gcaatggctc cttctcctaa atgagtagca atcaccgctc
    10861 caaaatgaac ctcttcaatg tcatattgat aaccactgtc ttgtaagagt cgtttcaaat
    10921 aatcagcttt gtcttgtgct tttgagtgaa taatagacac attatactga ccgtctgcta
    10981 tcaagtcatt taaaatctca accaagcgtt tcatggcttt cttttctgtt ctcacttttt
    11041 cataaacaac gatttttccc tcttcatcaa agcgtaaaat aggtttaata ctcaaaagat
    11101 ttccaagaag agcagaccca tttgacaggc gtccgccttt aaccagatga ttaaggtcat
    11161 caaccataat aaaggctgtc gtcccatcaa tctgttcttg cagcttgttt aaaatagctt
    11221 gaaatgtcat tccttgacgt gaccaatcta gcacattttt taccatacta cctaaaggag
    11281 cagatgtgat cttactgtct ggaaatgcca tttcaatctc aggatgttct tcagctaaaa
    11341 attgaatatt ttgccaaaag cctgagattc ctccagctaa gaacaagcca ataacatgtg
    11401 tataaccctt agaagacaaa agccctaaaa gattatctag ttcagataaa cttggctgac
    11461 ttgtcttggg aagattttgg gagtcagcca tcttttgata aaaatcatct atactcaaat
    11521 ttcgaccttc aaaataggtc tcatcgtcaa tgataactgg tatatcaagg ctaaaaatag
    11581 ctttatcttg tttcagatct gttggtagtg ttgctgtact gtcagtaatt actgctaatt
    11641 tcattaattt ttaaactcca aatgaatacc tggacgatcc aaggcaattt ctgtgacttc
    11701 ataagtcaat cgcttaacca tgtctagcaa aggagcagct aaggattcaa cttcctcttg
    11761 cgtaaattcg cttggtttat ccactaaacg atctaaaatg cgaaccattt gtgagatgac
    11821 cccactaata acaaattctt ctttgacaat aacaaattgc aatacagata caattactgt
    11881 ttctttttgc tgctcatctt tatcaatcaa ctgaaaagtc acttcaaaat tagtctctgg
    11941 tgttccgttt tctttttccc actctaaatt tctagcatca taatggtact ggttgacaaa
    12001 ttctttttca cgaactaatt gcatttttta ttctccttgg tatcaatatg atccattata
    12061 gcatagattt gaaaattatt aaagtagccc ctaaactact attttcgcat aacgttttaa
    12121 acagtgctat ctctcgaaca gatatctatt ttttcaccta tttatattta gtcgtcctta
    12181 tactgacatt atatcaaaaa aacacccgtt agtggtcttg tactgcacct caattaaaac
    12241 agaaccactg gtcaggtaag ttttaagtca aggataaaaa ggtttatcat taaggcctca
    12301 tcatgcttag ccaagtattt cgaactagct tgtcttagct ctagtcgctc aaacgtaaat
    12361 cctaggcgtt caatccgccg caaaacctct ccaactaacc catacttaaa cccatcgggc
    12421 ttaatcatcg ctccgccctt cttcttttat ccattataaa ataaaaacgt tttaacagac
    12481 aaaaaatagg aaagtaccag actattggat ttattttttt atctcaaact ccattaacga
    12541 tgttgagtta aaggcattca aaacaacata gatcgtaagc taacaagatt ttttatcaat
    12601 tgaacctatc gtccccagtt ataccacgac ctcttctgga tgaggtcttt ttttatttgt
    12661 ctaatttcat caacgcttct tcccttcttt cttctatcac agctctccta ttgctcactt
    12721 cctcatgcag caacatatca tcaaaaaacc gttcattttt aagcacaacc actactgtat
    12781 ctcttgtgat gtctccattg caatcgcttg tttaatttta ttaaaaatta tttaaaaaat
    12841 taaatattat tttaagaact tgcaataata tatatctata ttatataatt tctttatcat
    12901 aaaattatat agaaaaaggt attcttatga gaaaaagatg ctattcaact tcagctgcag
    12961 tattggcagc agtgacttta tttgctctat cggtagatcg tggtgttata gcagatagtt
    13021 tttctgctaa tcaagagatt agatattcgg aagtaacacc ttatcacgtt acttccgttt
    13081 ggaccaaagg agttactcct acagcaaact tcactcaagg cgaagatgtt tttcacgctc
    13141 cttatgttgc taaccaagga tggtatgata ttaccaaaac attcaatgga aaagacgatc
    13201 ttctttgcgg ggctgccaca gcagggaata tgcttcactg gtggttagat caaaacaacg
    13261 aaaaaattga agcatatcta aaaaaacacc cagataaaca aaaaatcatg tttggtgatc
    13321 aagaattatt ggatgtaaga aaagttatta atactaaagg tgaccaaaca aatagcgagc
    13381 tttttaatta tttccgagat aaagctttcc ccggtttgtc agcacgccga attggagtta
    13441 tgcctgatct tgttttagat atgtttatca atggttatta cttaaatgtt tataagacac
    13501 agactactga tgtcaataga acctatcaag agaaagatcg ccgaggtggt atttttgacg
    13561 ccgtatttac aagaggtgat caaagtaagc tattgacaag tcgtcatgat tttaaagaaa
    13621 aaaatctcaa agaaatcagt gatctcatta agaaagagtt aaccgaaggc aaggctctag
    13681 gcctatcaca cacctacgct aacgtacgca tcaaccatgt tataaacctg tggggagctg
    13741 acttcgattc taacgggaac cttaaagcta tttatgtaac agactctgat agtaatgcat
    13801 ctattggtat gaagaaatac tttgttggtg ttaattccgc tggaaaagta gctatttctg
    13861 ctaaagaaat aaaagaagat aatattggtg ctcaagtact agggttattt acactttcaa
    13921 cagggcaaga tagttggaat cagaccaatt aaagcgtatc aataagaaag aatatcttca
    13981 acagatgatg gtattctaag gactaatact attgcaggga atttttttat agatgggcaa
    14041 cagcaaaaaa cattaataaa tcctataaaa atcgataagg atattgttac gattcaagag
    14101 ttcgatttta aaataagaaa atttttgatg gaatcaaaag aaattcattt gactaagtct
    14161 ccttatataa gggggagttt agaaattcac agtaaaaata ggaaacatga aaaaatcaat
    14221 ttatatgacg caaaacccaa tagcaccaga tctgatgtct taaaaaaata taaagacaat
    14281 aagactataa acatgaaaga tttcagccat tttgatattt atctttggac taaataagct
    14341 aaaagctttg gataaatatg atcaattgac ctataataac attgatacca aatctaaaac
    14401 tgctcagatt gaattgcgcc ccaaaagtta gacaaaaaat ctaataattg gggtgttttt
    14461 gtctggcatt aagtttagct aataaccttg cttaaatcac attaaaccaa taatggaaaa
    14521 agacatctcc aaataaatgc tatgatggaa ctatatgata gagtgatcaa ggttggcaat
    14581 atcaacacaa ctattatcat catttcctaa actctaaagg aattcgtccg tctatgtcac
    14641 gtaaaagaaa cagtcctgac aatggcatga tggaatcttt ctttggcatc ttaaagtcag
    14701 aaatgttcta tggttatgag aataactata aatcactaga agacttagaa caagcgatta
    14761 caaatgacat tgattattac aacgataaac atatcaaagt
//
LOCUS       DAUCLR010000040        14769 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.040, whole genome shotgun sequence.
ACCESSION   DAUCLR010000040 DAUCLR010000000
VERSION     DAUCLR010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14769
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.040"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            28..654
                     /gene="rplC"
                     /locus_tag="VGT87_001044"
     CDS             28..654
                     /gene="rplC"
                     /locus_tag="VGT87_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEQ3813906.1"
                     /translation="MTKGILGKKVGMTQIFTESGEFIPVTVIEATPNVVLQVKTVETD
                     GYEAVQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNIEGLEVGAELSVEQFEA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRLAG
                     RMGGNRVTVQNLEIVQVIPEKNVILIKGNVPGAKKSLITIKSAVKAAK"
     gene            678..1301
                     /gene="rplD"
                     /locus_tag="VGT87_001045"
     CDS             678..1301
                     /gene="rplD"
                     /locus_tag="VGT87_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEQ3813907.1"
                     /translation="MANVKLFDQTGKEVSSVELNDAIFGIEPNESVVFDVVISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSAKVAEDKFVAVEGLSFAAPKTAEFAKVLSALSIDTKVLVLVEEGN
                     EFAALSARNLPNVTVATAATASVLDIVNADKLLVTKEAISTIEEVLA"
     gene            1301..1597
                     /locus_tag="VGT87_001046"
     CDS             1301..1597
                     /locus_tag="VGT87_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEQ3813908.1"
                     /translation="MNLYDVIKKPVITEKSMIALEAGKYTFEVDTRAHKLLIKQAVEA
                     AFDGVKVASVNTVNVKPKAKRVGRYTGFTSKTKKAIITLTADSKAIELFAAEAE"
     gene            1615..2448
                     /gene="rplB"
                     /locus_tag="VGT87_001047"
     CDS             1615..2448
                     /gene="rplB"
                     /locus_tag="VGT87_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEQ3813909.1"
                     /translation="MGIKVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVSLKSKAGRN
                     NNGRITVRHQGGGHKRHYRLIDFKRNKDGVEAVVKTIEYDPNRTANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPDADIKVGNALPLANIPVGTVVHNIELKPGKGGELVRAAGA
                     SAQVLGQEGKYVLVRLQSGEVRMILGTCRATIGTVGNEQQSLVNIGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            2587..2865
                     /gene="rpsS"
                     /locus_tag="VGT87_001048"
     CDS             2587..2865
                     /gene="rpsS"
                     /locus_tag="VGT87_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEQ3813910.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRR"
     gene            2881..3225
                     /gene="rplV"
                     /locus_tag="VGT87_001049"
     CDS             2881..3225
                     /gene="rplV"
                     /locus_tag="VGT87_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEQ3813911.1"
                     /translation="MAEITSAKAMARTVRVSPRKTRLVLDLIRGKKVADAIAILKFTP
                     NKAARVIEKTLNSAIANAENNFGLEKANLVVSETFANEGPTMKRFRPRAKGSASPINK
                     RTTHVTVVVSEK"
     gene            3238..3891
                     /gene="rpsC"
                     /locus_tag="VGT87_001050"
     CDS             3238..3891
                     /gene="rpsC"
                     /locus_tag="VGT87_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEQ3813912.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFINK
                     ELADASVSTIEIERAVNKVIVSLHTAKPGMVIGKGGANVDALRGQLNKLTGKQVHINI
                     IEIKQPDLDAHLVGENIARQLEQRVAFRRAQKQAIQRTMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            3895..4308
                     /gene="rplP"
                     /locus_tag="VGT87_001051"
     CDS             3895..4308
                     /gene="rplP"
                     /locus_tag="VGT87_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEQ3813913.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVSFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            4318..4524
                     /locus_tag="VGT87_001052"
     CDS             4318..4524
                     /locus_tag="VGT87_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEQ3813914.1"
                     /translation="MKLQEIKDFVKELRGLSQEELAKKENELKKELFDLRFQAAAGQL
                     EKTARLDEVKKQIARVKTVQSEMK"
     gene            4550..4810
                     /gene="rpsQ"
                     /locus_tag="VGT87_001053"
     CDS             4550..4810
                     /gene="rpsQ"
                     /locus_tag="VGT87_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEQ3813915.1"
                     /translation="MERNQRKTLYGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEKAVII"
     gene            4835..5203
                     /gene="rplN"
                     /locus_tag="VGT87_001054"
     CDS             4835..5203
                     /gene="rplN"
                     /locus_tag="VGT87_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEQ3813916.1"
                     /translation="MIQQETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKTGARRPDGSYIKFDDNAAVIIRDDKTPRGTRIFGPV
                     ARELREGGYMKIVSLAPEVL"
     gene            5282..5587
                     /gene="rplX"
                     /locus_tag="VGT87_001055"
     CDS             5282..5587
                     /gene="rplX"
                     /locus_tag="VGT87_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEQ3813917.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLKALPKVNKVIVEGVGMIKKH
                     QKPNTENPQGAIVEKEAPIHVSNVQVLDKNGVAGRVGYKVVDGKKVRYSKKSGEVLD"
     gene            5611..6153
                     /gene="rplE"
                     /locus_tag="VGT87_001056"
     CDS             5611..6153
                     /gene="rplE"
                     /locus_tag="VGT87_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEQ3813918.1"
                     /translation="MANRLKEKYTNEVIPALTEKFNYTSVMAVPKVEKIVLNMGVGDA
                     VSNAKNLEKAAAELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEISFDDVDKVRGLDIVIVTT
                     ANTDEESRELLKGLGMPFAK"
     gene            6169..6354
                     /locus_tag="VGT87_001057"
     CDS             6169..6354
                     /locus_tag="VGT87_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEQ3813919.1"
                     /translation="MAKKSMIAKNKRPAKHSTQAYTRCEKCGRPHSVYRKFKLCRVCF
                     RELAYKGQIPGVVKASW"
     gene            6505..6903
                     /gene="rpsH"
                     /locus_tag="VGT87_001058"
     CDS             6505..6903
                     /gene="rpsH"
                     /locus_tag="VGT87_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEQ3813920.1"
                     /translation="MVMTDPIADFLTRIRNANQVKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEVIEDDKQGIIRVFLKYGKNGERVITNLKRISKPGLRVYAKRDDMPKVLNGLG
                     IAIISTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            7106..7642
                     /gene="rplF"
                     /locus_tag="VGT87_001059"
     CDS             7106..7642
                     /gene="rplF"
                     /locus_tag="VGT87_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEQ3813921.1"
                     /translation="MSRIGNKVITMPAGVELTNNNNVITVKGPKGELTREFNKNIEIK
                     VEGTEITVVRPNDSKEMKTIHGTTRANLNNMVVGVSEGFKKDLEMKGVGYRAQLQGTK
                     LVLSVGKSHQDEVEAPEGITFTVANPTSISVEGINKEVVGQTAAYIRSLRSPEPYKGK
                     GIRYVGEYVRLKEGKTGK"
     gene            7747..8103
                     /gene="rplR"
                     /locus_tag="VGT87_001060"
     CDS             7747..8103
                     /gene="rplR"
                     /locus_tag="VGT87_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEQ3813922.1"
                     /translation="MISKPDKNKIRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKDVSKGTKTEQAVVVGKLVAERAVAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            8122..8616
                     /gene="rpsE"
                     /locus_tag="VGT87_001061"
     CDS             8122..8616
                     /gene="rpsE"
                     /locus_tag="VGT87_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEQ3813923.1"
                     /translation="MAFKDNAVELEERVVAINRVTKVVKGGRRLRFAALVVVGDGNGR
                     VGFGTGKAQEVPEAIRKAVEAAKKNMIEVPMVGTTIPHEVYTNFGGAKVLLKPAVEGS
                     GVAAGGAVRAVIELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEVAALRGISV
                     SDLA"
     gene            8631..8813
                     /gene="rpmD"
                     /locus_tag="VGT87_001062"
     CDS             8631..8813
                     /gene="rpmD"
                     /locus_tag="VGT87_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002959367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEQ3813924.1"
                     /translation="MAQIKITLTKSPIGRKPEQRKTVVALGLGKLNSSVVKEDNAAIR
                     GMVTAISHLVTVEDVK"
     gene            9028..9468
                     /gene="rplO"
                     /locus_tag="VGT87_001063"
     CDS             9028..9468
                     /gene="rplO"
                     /locus_tag="VGT87_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEQ3813925.1"
                     /translation="MKLHELKAAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRIPKRGFTNINTKEYALVNLDQLNVFDDGTEVTPAILKDAGIV
                     RAEKSGVKVLGNGELTKKLTVKAAKFSKSAEAAIIAKGGSIEVI"
     gene            9485..10789
                     /gene="secY"
                     /locus_tag="VGT87_001064"
     CDS             9485..10789
                     /gene="secY"
                     /locus_tag="VGT87_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEQ3813926.1"
                     /translation="MFLKILKDALKIKTVRNKIFFTIFIILVFRIGTHITVPGVNAKS
                     LEQLSELPFLNMLNLVSGNAMRNFSVFSMGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYISLVLAFAQSIGITAGFNTLSNVALVKTPDIKTYLLIGALLTTG
                     SVIVTWLGEQITDKGFGNGVSMIIFAGIISSIPSAIATIREDYFVNVKASDLHSSYLI
                     VGILIIAVLAIVFFTTYVQQAEYKIPIQYTKLMQGAPTSSYLPLKVNPAGVIPVIFAS
                     SITTIPSTIIPFVQNGRDLPWLNRLQEIFNYQTPVGMIVYALLIILFSFFYTFVQVNP
                     EKTAENLQKNSSYIPSVRPGRETEQFMSALLKKLATVGAIFLAFISLAPIAAQQALNL
                     SSSIALGGTSLLILISTGIEGMKQLEGYLLKRKYVGFMNTAE"
     gene            10939..11577
                     /locus_tag="VGT87_001065"
     CDS             10939..11577
                     /locus_tag="VGT87_001065"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEQ3813927.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEEFGVAHISTGDMFRAAMANQTE
                     MGRLAKSYIDKGELVPDEVTNGIVKERLAEDDIAEKGFLLDGYPRTIEQAHALDATLE
                     ELGLRLDGVINIKVDPSCLVERLSGRIINRKTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNMAQGEPILEHYRKLGLVTDIEGNQEITDVFADVEKALLELK"
     gene            11695..11913
                     /gene="infA"
                     /locus_tag="VGT87_001066"
     CDS             11695..11913
                     /gene="infA"
                     /locus_tag="VGT87_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEQ3813928.1"
                     /translation="MAKEDVIEIEGKVVETMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILVGDRVTVEMSPYDLTRGRITYRFK"
     gene            11939..12055
                     /gene="rpmJ"
                     /locus_tag="VGT87_001067"
     CDS             11939..12055
                     /gene="rpmJ"
                     /locus_tag="VGT87_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEQ3813929.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPTNPKHKQRQG"
     gene            12073..12438
                     /gene="rpsM"
                     /locus_tag="VGT87_001068"
     CDS             12073..12438
                     /gene="rpsM"
                     /locus_tag="VGT87_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEQ3813930.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDIR
                     VKDLTSDQEDAIRREVDAIKVEGDLRREVNMNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            12456..12839
                     /gene="rpsK"
                     /locus_tag="VGT87_001069"
     CDS             12456..12839
                     /gene="rpsK"
                     /locus_tag="VGT87_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEQ3813931.1"
                     /translation="MAKPTRKRRVKKNIESGVAHIHATFNNTIVMITDVHGNALAWSS
                     AGALGFKGSRKSTPFAAQMAAEAAAKSAQEHGLKTVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            12885..13823
                     /locus_tag="VGT87_001070"
     CDS             12885..13823
                     /locus_tag="VGT87_001070"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEQ3813932.1"
                     /translation="MIEFEKPIITKIDENKDYGRFVIEPLERGYGTTLGNSLRRVLLS
                     SLPGAAVTSIKIDGVLHEFDTIPGVREDVMQIILNVKGLAVKSYVEDEKIIELEVEGP
                     AEVTAGDILTDSDIELVNPDHYLFTIAEGHSLRATMTVAKKRGYVPAEGNKKDDAPVG
                     TLAVDSIYTPVKKVNYQVEPARVGSNDGFDKLTIEIMTNGTIIPEDALGLSARVLIEH
                     LNLFTDLTEVAKATEVMKETEKVNDEKVLDRTIEELDLSVRSYNCLKRAGINTVFDLT
                     EKSEPEMMKVRNLGRKSLEEVKVKLADLGLGLKNDK"
     gene            13838..14224
                     /gene="rplQ"
                     /locus_tag="VGT87_001071"
     CDS             13838..14224
                     /gene="rplQ"
                     /locus_tag="VGT87_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEQ3813933.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAYIRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
BASE COUNT         4853 a         2697 c         3096 g         4123 t
ORIGIN      
        1 attattaaga aaaggaaata ttttctcatg acaaaaggaa tcttagggaa aaaagtggga
       61 atgactcaaa tcttcactga atcaggcgaa ttcatccctg ttactgtcat tgaagcaact
      121 ccaaacgttg tgcttcaagt taaaactgtt gaaacagacg gttatgaagc agttcaggtt
      181 ggttttgatg acaaacgtga agtcttgagt aacaaacctg ccaaaggcca tgttgcaaaa
      241 gcaaacacag ctcctaagcg cttcattcgt gaattcaaaa acattgaagg cttagaagtt
      301 ggtgcagaat tatctgtaga acaatttgaa gctggtgatg ttgttgacgt cacagggaca
      361 tcaaaaggta aaggtttcca aggtgttatc aaacgccatg gtcaatcacg tggtcctatg
      421 gctcacggtt ctcgttacca tcgtcgccca ggttctatgg gacctgttgc gcctaaccgc
      481 gttttcaaaa acaaacgctt ggcaggacgt atgggtggta accgtgtaac agttcaaaac
      541 cttgaaattg tacaagttat cccagaaaag aacgttatcc ttattaaagg taacgtacca
      601 ggtgctaaga aatctcttat cactatcaag tcagcagtta aagctgctaa ataataagaa
      661 aggagaaaac agttaaaatg gcaaacgtaa aactatttga ccaaactggt aaagaagtta
      721 gctcagttga attaaacgac gctatcttcg gtatcgaacc aaacgaatca gttgtttttg
      781 atgttgtaat tagccaacgc gctagccttc gccaaggtac tcatgcggtt aaaaaccgtt
      841 cagcagtatc aggtggtgga cgtaaaccat ggcgtcaaaa aggaactgga cgcgctcgtc
      901 aaggttctat ccgctcacca caatggcgtg gtggtggtgt tgtctttgga ccaactcccc
      961 gttcatacgg atacaaactt ccacaaaaag ttcgtcgcct tgcgttgaaa tcagtttact
     1021 cagcaaaagt tgctgaagat aaatttgtag ctgtagaagg cctttcattt gcagcaccaa
     1081 aaactgctga atttgcaaaa gtgctttcag ctcttagcat tgatacaaaa gtacttgttc
     1141 ttgttgaaga aggaaatgaa tttgcagcac tttctgcacg taaccttcca aacgtaactg
     1201 ttgcaactgc agcaactgca agtgttcttg atatcgtgaa cgcagacaaa cttcttgtta
     1261 ctaaagaagc aatctctaca attgaggagg ttcttgcata atgaatttgt acgacgtaat
     1321 caaaaaacca gttatcactg agaagtcaat gattgctctt gaagcaggta aatacacttt
     1381 cgaagttgat actcgtgcac acaaactttt gatcaaacaa gctgttgaag ctgcttttga
     1441 cggagttaaa gttgcaagtg taaatactgt taacgttaaa ccaaaagcaa aacgcgttgg
     1501 tcgttacaca ggtttcactt caaaaactaa aaaagctatc atcactctta cagctgattc
     1561 taaagcaatc gagttgttcg cagctgaagc tgaataatct aaggaggaaa taacgtgggt
     1621 attaaagttt ataaaccaac gacaaatggc cgtcgtaaca tgacttcttt ggatttcgcg
     1681 gaaatcacaa caagcacgcc tgagaaatcc ttgcttgttt ctcttaagag caaagctggt
     1741 cgtaacaaca atggtcgcat cacagttcgt caccaaggtg gtggacacaa acgtcattac
     1801 cgtttgatcg acttcaaacg taacaaagat ggcgttgaag cagttgttaa aacaatcgaa
     1861 tatgatccaa accgtactgc aaacatcgca cttgtacatt acactgacgg tgtgaaagct
     1921 tacatcattg cacctaaagg tcttgaagta ggtcaacgta ttgtttctgg tccagatgcc
     1981 gacatcaaag ttggtaacgc acttccatta gcaaacattc ctgtcggtac agttgttcac
     2041 aatattgagt tgaaacctgg taaaggtgga gaacttgttc gtgcagctgg agcttctgct
     2101 caagtacttg gtcaagaagg taaatacgtt cttgttcgtc ttcaatcagg cgaagttcgt
     2161 atgattcttg gtacatgccg tgcaactatc ggtactgttg gtaacgaaca acaatcactt
     2221 gttaacattg gtaaagcagg acgtagtcgt tggaaaggta tccgcccaac agttcgtggt
     2281 tctgtaatga accctaacga tcacccacac ggtggtggtg aaggtaaagc accagttgga
     2341 cgtaaagcgc catcaactcc atggggtaaa ccagcgcttg gtcttaaaac tcgtaacaag
     2401 aaagctaaat cagacaaact tatcgttcgt cgtcgtaacg aaaaataatt ttagtaagtt
     2461 gcttaaaact tgctgaaata gcgttacaat agctttacta tttattccgc caactcggta
     2521 gagtttggag tcttgctctt gaattcaagc cgctgtggta cattatttaa aggagaaaac
     2581 tacaaaatgg gacgtagtct taaaaaagga cctttcgtcg atgagcattt gatgaaaaaa
     2641 gttgaagctc aagcaaacga cgaaaagaaa aaagtaatca aaacttggtc acgtcgttca
     2701 acgattttcc caagtttcat cggatataca atcgcagttt atgacggacg taaacatgta
     2761 cctgtttaca tccaagaaga catggtaggt cacaaacttg gtgaatttgc accaacgcgt
     2821 acttacaaag gtcacgcagc tgacgacaag aaaacacgtc gttaatagga ggaggacaca
     2881 atggcagaaa ttacttcagc taaagcaatg gctcgtacag tgcgtgtttc acctcgtaaa
     2941 acacgtttag tacttgatct tatccgtggt aagaaagttg ctgacgcaat cgcaatctta
     3001 aaattcactc caaacaaagc agctcgtgtt attgagaaaa ctcttaactc agcaattgct
     3061 aacgcagaaa acaactttgg tttggaaaaa gcaaacttgg tagtatctga aacattcgct
     3121 aacgaaggac caacaatgaa acgtttccgt ccacgtgcga aaggttcagc ttcaccaatc
     3181 aacaaacgta caactcacgt aactgtagtt gtatcagaaa aataaggagg taaaatcgtg
     3241 ggtcaaaaag tacatccaat tggtatgcgt gtcggaatca tccgtgactg ggatgcgaaa
     3301 tggtatgctg aaaaagaata cgcggattac cttcatgaag atcttgcaat ccgtaaattc
     3361 attaataaag aattagctga cgcatcagtt tcaactattg aaattgaacg tgcagtaaac
     3421 aaagttattg tttcacttca cactgctaaa ccaggtatgg ttatcggtaa aggtggcgca
     3481 aacgttgacg ctcttcgtgg tcaacttaac aaattaactg gaaaacaagt acacatcaac
     3541 atcatcgaaa tcaaacaacc tgatttagat gctcaccttg ttggtgaaaa tattgctcgt
     3601 caacttgagc aacgtgttgc tttccgtcgt gctcaaaaac aagcaatcca acgtacaatg
     3661 cgtgcaggag ctaaagggat taaaactcaa gtttctggtc gtttgaacgg tgctgatatc
     3721 gctcgtgctg aaggttattc agaaggaact gttccacttc acacgcttcg cgctgatatc
     3781 gactatgctt gggaagaagc tgacacaact tatggtaaac ttggcgttaa agtttggatt
     3841 taccgtggag aagttcttcc agctcgtaaa aacactaaag gaggcaaata acaaatgtta
     3901 gtacctaaac gtgttaaaca ccgtcgcgaa ttccgtggaa aaatgcgcgg tgaggctaaa
     3961 ggtggtaaag aagtttcatt cggtgaatac ggacttcaag ccacaactag ccactggatt
     4021 acaaaccgtc aaatcgaagc tgcccgtatt gcgatgactc gttacatgaa acgtggtggt
     4081 aaagtttgga ttaaaatctt cccacacaaa tcatacactg caaaagctat cggtgtacgt
     4141 atgggttctg gtaaaggggc acctgaaggt tgggtagcac cagttaaacg tggtaaagtg
     4201 atgtttgaaa ttgctggtgt ttctgaagaa attgctcgcg aagcattacg tcttgctagc
     4261 cacaaattac cagttaagtg caaattcgta aaacgtgaag cagaataagg agaagacatg
     4321 aaacttcaag aaattaaaga ttttgttaaa gagcttcgtg gattgtctca agaagaactt
     4381 gctaagaaag aaaacgaact caaaaaagaa ttgtttgatc ttcgtttcca agctgcagca
     4441 ggtcaacttg aaaagactgc tcgccttgac gaagttaaga aacaaattgc acgtgttaaa
     4501 actgtgcaat cagaaatgaa ataatagatt gggaaaggag aaattctaaa tggaacgtaa
     4561 tcaacgtaaa actctttacg gacgcgtagt gtctgacaag atggacaaaa caatcacagt
     4621 tgtagttgaa actaaacgta accacccagt ctatggtaaa cgtatcaact attctaaaaa
     4681 atataaagca catgacgaaa acaacgttgc taaagaaggc gatatcgttc gtatcatgga
     4741 aactcgccca ctttcagcta caaaacgttt ccgtcttgtg gaagtagtgg aaaaagctgt
     4801 tattatctaa tcaaactaaa aggagaaaat tgaaatgatt caacaagaaa ctcgcttgaa
     4861 agttgctgat aatagcggtg ctcgtgagat cttgactatc aaagtacttg gtggttcagg
     4921 acgtaaattc gctaacatcg gtgacgtaat cgttgcttct gtaaaacaag ctactcctgg
     4981 tggagcagtt aaaaaaggtg atgtggttaa agctgttatc gttcgtacaa aaactggtgc
     5041 acgccgtcca gacggttcat acatcaaatt tgacgacaat gctgctgtaa tcatccgtga
     5101 tgataaaact cctcgcggaa ctcgtatctt tggcccagtt gcacgcgaat tgcgtgaggg
     5161 tggctacatg aagatcgtat cacttgcacc agaagtactt taatctcagg ttaacaaacg
     5221 tagtccccta ggttttccta gggtgcccat cggggcgtaa gaaataatag gagaaaccag
     5281 aatgtttgta aaaaaaggcg acaaagttcg cgtaattgct ggcaaggaca aaggcactga
     5341 agctgtagtt cttaaagcac ttccaaaagt taacaaagtt attgttgaag gtgttggaat
     5401 gattaaaaaa caccaaaaac ctaacactga aaatcctcaa ggagctatcg tcgaaaaaga
     5461 agcacctatc catgtctcta atgttcaagt attagataaa aatggtgtag ctggacgtgt
     5521 tggttataaa gttgttgacg gtaaaaaagt acgttacagc aaaaaatcag gcgaagtgct
     5581 tgattaatca cgaaggaaag gagaagcata atggcaaatc gtttaaaaga aaaatatact
     5641 aacgaagtaa ttcctgcgtt gacagagaaa ttcaattaca catcagttat ggctgtgcca
     5701 aaagttgaga aaattgttct caatatgggt gttggtgatg ctgtatccaa cgcaaaaaac
     5761 ctagagaaag ctgcagctga attagcactt atctcaggtc aaaaaccact tattactaaa
     5821 gctaagaaat caatcgctgg cttccgtctt cgtgaaggtg tagcgatcgg tgcaaaagtt
     5881 accctccgtg gcgaacgtat gtacgaattc ctagacaaat tggttagcgt ttcacttcct
     5941 cgtgtacgtg acttccatgg cgttccaaca aaatcgtttg atggacgcgg aaactacact
     6001 ctcggtgtga aagaacaact tatcttccca gaaatcagct ttgatgatgt tgataaagta
     6061 cgtggtcttg atattgtaat cgtcactact gcaaatactg acgaagaatc acgtgaattg
     6121 cttaagggcc ttggaatgcc ttttgcaaaa taataggggg taaataaatt ggctaaaaaa
     6181 tctatgattg ctaagaacaa acgtcctgca aaacactcta cacaagctta tactcgctgt
     6241 gaaaaatgtg gacgtccaca ttcagtttac cgcaagttta aactttgtcg tgtttgcttc
     6301 cgtgaattgg cttacaaagg tcaaattcca ggtgttgtca aagcttcttg gtaaaaatat
     6361 gacacaaaac ttaatttgtt cttcagatta agtcttgtgc ttaattaaaa cggtgtgagc
     6421 ataagctgat accaaagtcc tttaaggaca acattaacta gtaagtgata gcatcaaact
     6481 gctagtaaga ggagaatata aaaaatggtt atgactgacc caattgcaga ctttttaaca
     6541 cgtatccgta atgctaatca agtaaaacac gaagtgttag aagtacctgc ttcaaacatt
     6601 aaaaaaggga ttgctgagat tcttaaacgt gaaggttttg taaaaaatgt tgaagttatc
     6661 gaagatgata aacaaggcat cattcgtgtg ttcttaaaat acggaaaaaa tggtgagcgt
     6721 gttatcacta acttaaaacg tatttcaaaa ccaggtcttc gcgtttacgc aaaacgtgat
     6781 gacatgccta aagttcttaa cggacttgga attgcaatca tttcaacatc agaaggtctt
     6841 ttgactgaca aagaagctcg ccaaaagaat gttggtggag aagtgatcgc ttacgtttgg
     6901 taatttaaga tactaagagc gtcgtggaca aagtgaaaat aggaaatctg acgtagcgtg
     6961 tttacactca aggaagattt atctttttca cacagaccac agctcgtgtt caattttgaa
     7021 atataaattg aagtatatca ttctatcaaa cccccgtgaa aactagtcgc tatgcggctt
     7081 gacaatttaa caggagaaaa taaacatgtc acgtattggt aataaagtaa ttactatgcc
     7141 tgcaggcgtt gaattaacaa ataacaacaa tgttattact gttaaaggcc ctaaaggcga
     7201 actcactcgt gagttcaaca aaaatattga aatcaaagtt gaagggactg aaatcacagt
     7261 tgtacgtcct aacgactcaa aagaaatgaa aacaatccat gggacaaccc gtgctaactt
     7321 gaataacatg gttgtaggtg tttctgaagg tttcaaaaaa gatcttgaaa tgaagggtgt
     7381 cggttaccgt gctcaacttc aaggtactaa acttgtcctt tcagtaggta aatctcacca
     7441 agacgaagtt gaagctccag aaggaattac tttcactgtt gctaacccaa cttcaatctc
     7501 agttgaagga atcaacaaag aagttgttgg tcaaacagct gcttacatcc gtagcttgcg
     7561 ttcaccagag ccttacaaag gcaaagggat ccgttacgtt ggtgaatacg tacgccttaa
     7621 agaaggtaaa acaggtaaat aatgttctta gtcgggtatg ggaagtattg caccttaaca
     7681 tgctcgataa gttcaatcga caacttgtca atgagacaaa ttgattaaaa ttaagaggtg
     7741 aaaattgtga tttcgaaacc agataaaaac aaaatccgcc aaaaacgcca ccgtcgcgtc
     7801 cgcggtaaac tctctggaac tgctgatcgc ccacgtttga acgtattccg ttctaataca
     7861 ggcatctacg ctcaagttat tgatgacgta gcgggtgtaa cgctcgcaag tgcatcaact
     7921 cttgataaag acgtttctaa aggaacaaaa acagaacaag ccgttgtagt cggcaaactt
     7981 gttgctgaac gtgcagtggc taaaggtatt tctgaagtgg tgtttgaccg cggtggatat
     8041 ctctatcacg gacgtgttaa agccttggct gatgcagctc gtgaaaacgg attgaaattc
     8101 taatagggag gacacaaaat aatggcattt aaagataatg cagttgaact tgaagaacgc
     8161 gttgttgcga ttaaccgcgt tacaaaagtt gttaaaggtg gacgtcgtct tcgctttgca
     8221 gctcttgtag ttgttggtga tggaaatggt cgtgttggat ttggtactgg taaagctcaa
     8281 gaagttccag aagctatccg taaagcagtt gaagctgcta agaaaaacat gatcgaagta
     8341 ccaatggttg gtacaacaat ccctcacgaa gtttacacta acttcggtgg agctaaagta
     8401 ttattgaaac cagctgtaga aggttctgga gttgctgctg gtggtgcagt tcgtgccgtc
     8461 atcgaattag caggtgttgc tgatattact tcaaaatctc ttggttcaaa cactccaatc
     8521 aacattgttc gtgcaactgt tgaaggattg aaacaactta aacgtgcaga agaagttgcc
     8581 gcacttcgtg gcatctcagt ttctgactta gcataagaaa ggggatcatc atggctcaaa
     8641 ttaaaattac tttgactaag tctcctatcg gacgtaagcc agaacaacgt aaaactgttg
     8701 ttgctcttgg acttggtaaa ttaaactctt cagtagtcaa agaagataat gctgctatcc
     8761 gtggtatggt gacagctatt tctcacttgg ttaccgttga agatgttaaa taagttataa
     8821 ctgtgaaatc tctgatttca taattaaaaa cgagcacaaa accgactctt gtagtttgct
     8881 tttttggcaa caaacagtag tcggttagtg cgtatctgta ttaagttatt ttggataact
     8941 taagtatcat taatgtatag gcgagtttct atagggagtc cttttccctc atagaggcgc
     9001 tagcatttta caaaaaaagg agaaaaaatg aaacttcatg aattaaaagc tgctgaaggc
     9061 tcacgtaaag tacgtaaccg tgttggtcgt gggacatcat caggtaatgg taaaacatct
     9121 ggtcgcggtc aaaaaggtca aaaagctcgt agcggtggcg gtgtacgttt aggttttgaa
     9181 ggtggacaaa caccattgtt ccgtcgtatt ccaaaacgtg gctttacaaa tattaatact
     9241 aaagaatatg cacttgtgaa tcttgatcaa ctgaatgttt ttgatgatgg tacagaagtg
     9301 actccggcta ttttaaaaga tgctggtatt gtccgagctg aaaaatcagg cgttaaagtg
     9361 cttggtaacg gtgaattgac taaaaaattg actgtaaaag cagctaagtt ctcaaaatct
     9421 gctgaagcag ctattattgc taaaggtggt tcaatcgaag tcatctaatg aggggatact
     9481 cattatgttc ttaaaaatac taaaagacgc gttaaagata aagactgtac ggaataaaat
     9541 tttctttact atatttatca ttcttgtatt ccgaattggg acacatatta ccgtacctgg
     9601 tgtaaatgcc aagagcttag agcaattaag tgaactccct ttcttaaata tgttaaactt
     9661 agttagtggt aatgcgatga gaaatttctc agtattttca atgggggtta gcccatatat
     9721 tactgcttct atcgttgttc agttattgca gatggatatt ttacctaagt ttgttgagtg
     9781 gggcaaacaa ggtgaggttg gacgtcgtaa attaaatcaa gcgacgcgct atatttcact
     9841 tgttttggct tttgcccaat ccattggtat cactgcaggg tttaatactt tgtcgaacgt
     9901 tgcacttgtt aagacaccag acatcaaaac atatttactg atcggggcat tactgacaac
     9961 aggtagcgtt attgttacct ggcttggaga acaaatcaca gataaaggat ttggtaatgg
    10021 cgtatcaatg attatctttg cgggtattat ttcgtctatt ccaagtgcaa ttgcaacaat
    10081 tcgtgaagac tattttgtaa atgttaaggc aagtgattta cactcgtctt atctcattgt
    10141 tgggatttta atcatagctg ttcttgctat tgttttcttt acaacatatg tccaacaagc
    10201 ggaatataaa attccaatcc aatacacaaa gctaatgcaa ggtgcaccta caagttcata
    10261 tcttccatta aaagtaaatc cagccggcgt tattcccgtt atctttgcta gctcgattac
    10321 aactatccca agtacgatta ttccttttgt tcaaaatggt agagatttac cgtggttaaa
    10381 ccgtttgcaa gaaattttta attatcaaac tccagtcgga atgatagttt acgccttgtt
    10441 gattatattg ttctcattct tctatacctt tgtacaagtt aatcctgaga agacagcaga
    10501 aaatcttcag aagaattcct catatatacc aagtgttcgc cctggacgtg agacagaaca
    10561 atttatgtcc gcattgctaa aaaaactagc aaccgtagga gctatcttct tagcatttat
    10621 ctctttagcg ccaatagcag cacaacaagc tctcaacctt tcttctagta ttgctttagg
    10681 tggaactagt ttgctcattt tgatttcaac tggtatcgaa ggtatgaaac agcttgaggg
    10741 atatcttcta aagagaaaat atgtcggatt tatgaataca gcagaataga atacaggttg
    10801 acattgtcaa ccttctattt tgtttttaaa tgacaataag acttggttat catcaaacga
    10861 tttcttgttg ccatttgaaa acaaaaacca aggaaaataa tttcctacta tgtatttatt
    10921 ttaaaagagg aggaaaagat gaatctttta atcatgggtt tgccgggagc tggtaaaggg
    10981 acccaagcag ctaagattgt tgaagaattt ggtgttgctc acatctcaac gggggatatg
    11041 ttccgcgccg caatggctaa tcaaaccgaa atgggacgtt tagctaaaag ttatattgat
    11101 aaaggtgaat tggttcctga tgaagtcaca aacgggattg taaaagagcg cttagctgag
    11161 gatgatatcg cagaaaaagg ttttttgctt gatgggtatc cacgtactat tgaacaagca
    11221 cacgccttag atgcaacact tgaagaacta ggactccgct tagatggtgt tattaatatt
    11281 aaagtggatc catcatgtct tgtagagcgt ttgagtggtc gtattatcaa tcgtaaaact
    11341 ggtgaaacgt tccataaagt gttcaaccca ccagtagatt ataaagaaga agattactat
    11401 caacgtgaag atgataagcc tgaaactgtt aaacgtcgtt tggacgtcaa tatggctcaa
    11461 ggagaaccta ttcttgaaca ctatcgtaag cttggtcttg ttacagatat tgaaggtaat
    11521 caagaaataa cagacgtttt tgcagatgtt gaaaaagcgt tgctagaact caaataaatt
    11581 atactaatct acttgcataa gtagattagt aatgatatac tagtttagtc tgacttataa
    11641 ttgttacctc tgtgctcaga ggacatcaaa tcgaaattta gggggtactt ttgcgtggca
    11701 aaagaagacg tgattgaaat tgaaggcaaa gtagtagaaa cgatgccaaa tgcaatgttt
    11761 actgttgaat tggaaaatgg acatcaaatt ctagcaactg tatcaggaaa aatccgcaaa
    11821 aattacattc gtattttagt aggtgaccgt gttacagtag aaatgagtcc ttatgacttg
    11881 acacgtggac gcatcacata ccgctttaaa taatcgaaat aattggaggg attaaaacat
    11941 gaaggtaaga ccatcggtta aaccaatttg cgaatactgt aaagttattc gccgtaatgg
    12001 tcgtgttatg gtaatttgtc cgacaaatcc aaaacacaaa caacgtcaag gataaaatag
    12061 aaaggagaaa aaatggctcg tattgctgga gttgatattc caaatgataa acgcgtagta
    12121 atttcactta cttatgttta cggaattggt cttgcaacat ctaaaaaaat cttagcagct
    12181 gcaggtattt ctgaagatat ccgtgttaaa gatttaacat cagatcaaga agatgctatc
    12241 cgtcgtgaag tggatgcaat caaagttgaa ggtgaccttc gacgtgaagt aaacatgaac
    12301 attaaacgtt tgatggaaat cggttcatac cgtggaatcc gtcatcgtcg tggacttcct
    12361 gtccgtggac aaaatactaa aaacaatgct cgcactcgta aagggaaagc tgttgcgatt
    12421 gcaggtaaga aaaaataaaa taggaggtaa aacaattggc taaaccaaca cgtaaacgtc
    12481 gtgtgaaaaa gaacatcgaa tctggtgttg cacacattca cgctacattt aataacacta
    12541 ttgttatgat tacagatgtg catggtaatg ctcttgcatg gtcatcagct ggtgctcttg
    12601 gtttcaaagg ttctcgtaaa tctactccat ttgctgctca aatggctgca gaagcagctg
    12661 caaaatctgc acaagaacac ggactaaaaa ctgttgaagt gactgtaaaa ggtcctggtt
    12721 caggtcgtga atcagctatt cgtgctctag cagctgcagg tcttgaagta actgcaattc
    12781 gtgatgtgac ccctgtgcca cataatggtg ctcgtcctcc aaaacgtcgt cgtgtataat
    12841 catataacaa atagtacaca agtttcgttt cgaggggtga aaaaatgatt gagtttgaaa
    12901 aaccaataat aacaaaaatt gatgaaaata aagattacgg tagatttgtc atcgaaccac
    12961 ttgaacgtgg ctacggaaca actctaggta attctcttcg tcgtgtactc ttgtcttcac
    13021 ttccaggtgc agcagtaaca tcaattaaaa ttgatggagt actacacgaa tttgatacaa
    13081 tcccaggtgt acgtgaagat gtcatgcaaa ttatccttaa tgttaaggga cttgctgtga
    13141 aatcatacgt cgaagacgaa aagattatcg aacttgaagt agaaggacca gcagaggtaa
    13201 cagctggtga tattcttact gacagtgata tcgaacttgt taacccggat cattatcttt
    13261 ttacaatcgc tgaaggacat tctctacgtg caacaatgac tgttgctaaa aaacgtggtt
    13321 atgtgccagc agaaggcaac aaaaaagatg atgcacctgt gggtacattg gcagtagatt
    13381 caatctatac gccagttaaa aaagttaatt atcaagttga acctgcccgt gtaggtagca
    13441 atgatggctt tgataaatta acgattgaaa tcatgacaaa cggaacaatt attcctgaag
    13501 atgctttagg tctatctgct cgagttttga tcgaacactt aaacctgttt actgatttaa
    13561 cagaggttgc aaaagctacc gaagtaatga aagaaactga aaaagtgaac gatgaaaagg
    13621 tacttgaccg caccatcgag gagcttgatt tatccgtacg ctcatataac tgtttaaaac
    13681 gtgcaggcat taacactgtc ttcgacttaa cagaaaaatc tgagcctgaa atgatgaaag
    13741 tccgtaatct tggacgtaag agccttgaag aagttaaggt taaacttgct gacttaggtc
    13801 tcggactaaa aaacgataaa taatatagga ggacaaaatg gcttaccgta aactaggacg
    13861 cactagctca caacgtaaag caatgcttcg tgatttgacg acagatcttt tgatcaacga
    13921 atcaattgta acgacagaag cacgtgcaaa agaaatccgt aaaacagttg aaaaaatgat
    13981 tactcttggt aaacgtggtg atcttcatgc tcgtcgccaa gcagctgcat acattcgtaa
    14041 cgaaatcgca tcagaaaatt atgatgaagc taccgataaa tacacatcaa cgacagctct
    14101 tcaaaaactt ttctcagaaa ttgcacctcg ttatgcagaa cgtaacggtg gatacactcg
    14161 tattcttaaa acagaaccac gccgtggaga tgctgctcca atggcaatta ttgaattagt
    14221 ttaattttta tcaattttgt tgagtgttat gatgatggag taatcgagag attattctta
    14281 gtctagctct ggtctaccgc taggattttt cctagcggga acactcatca tattgttaat
    14341 atgtaacgct tgtttatgaa aggtatctaa gctatagaaa ctttttataa gcaggcgttt
    14401 tgttgtatta tgaggtgacc cccccgcttt taagctgagc aaaacaataa cacagaagcc
    14461 tcttctgatg cgtttataaa gttttctcag agacaataag gtcattagaa taagaaaacc
    14521 ttatgcaggt gtttaaagac ctctcatggg caaataaacg gtgtaacccc ctcatttaaa
    14581 tgtccgaaaa aaacttgcat cgcaaacaga tgctcaaact atattgttag ttttatctcc
    14641 tattcaatat tttattaata agtattttaa aatcctcttt taatttggag gtgaaaatat
    14701 cttttttact tttaaaatct gtcactttaa gccgtttcca acaaaattgt aaaaaagtac
    14761 aaaaaaagt
//
LOCUS       DAUCLR010000041        14637 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.041, whole genome shotgun sequence.
ACCESSION   DAUCLR010000041 DAUCLR010000000
VERSION     DAUCLR010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14637
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.041"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..704
                     /locus_tag="VGT87_001072"
     CDS             <1..704
                     /locus_tag="VGT87_001072"
                     /inference="COORDINATES: protein motif:HMM:NF013550.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_164232585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEQ3813934.1"
                     /translation="TGPEGPQGKTGPAGPAGPRGEQGLQGDRGEVGPRGEQGPAGPAG
                     KDGEKGDQGPMGPRGDRGEAGPAGKDGERGPVGPAGKDGQDGKDGLPGKDGQNGKDGL
                     PGKDGKDGQDGKDGLPGKDGQDGKDGQPGKPAPKTPEVPQKPDTAPHTPKTPQIPGQS
                     KDVTPAPQNPSNRGLNKPQTQGGNQLAKTPAAHDTHRQLPATGETTNPFFTAAAVAIM
                     TTAGVVAVAKRQENN"
     gene            874..1329
                     /gene="msrB"
                     /locus_tag="VGT87_001073"
     CDS             874..1329
                     /gene="msrB"
                     /locus_tag="VGT87_001073"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEQ3813935.1"
                     /translation="MKRSFPWKHPDELKQRIGDLSYEVTQHAATESPFTGEYDNFFEK
                     GIYVDIVSGEVLFSSLDKFNSGCGWPAFSKPIENRMVTNHDDSSYGMRRVEVKSREAG
                     SHLGHVFSDGPKEAGGLRYCINSAALKFIPYEQMEKEGYAQWLTLFDET"
     gene            1459..2478
                     /locus_tag="VGT87_001074"
     CDS             1459..2478
                     /locus_tag="VGT87_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEQ3813936.1"
                     /translation="MSTHLRKLPGLLLCLLLALPAWCLGRLFPIIGAPVFAILLGMLL
                     ALFYEHRDKTKEGISFTSKCILQTAVVLLGFGLNLTQVMAVGMQSLPIIISTIATALL
                     VAYGSQKWLRLDVNTATLVGVGSSICGGSAIAATAPVIKAKDDEVAKAISVIFLFNML
                     AALLFPSLGQLLGLSNEGFAIFAGTAVNDTSSVTATATAWDAIHHSNTLDGATIVKLT
                     RTLAILPITLGLSLYRAKKEHDIVTEENFSLRKSFPRFILFFLLASLITTLMTSLGVS
                     ADSFHSLKTLSKFFIVMAMAAIGLNTNLVKLIKTGGQAILLGAICWVAITLVSLAMQL
                     SLGIW"
     gene            2685..3110
                     /locus_tag="VGT87_001075"
     CDS             2685..3110
                     /locus_tag="VGT87_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEQ3813937.1"
                     /translation="MKRKFLIGSHGRLASGLQSSIDILAGMGQALETIDAYVDDSDYT
                     SQIDDFIAGVAADEQGLIFTDLLGGSVNQKMVTAVMNSGKDNIFLITNSNLATLLSLV
                     FLKPGEALTKDEIVTVINESQVQLVDLVPETNSEDDFFD"
     gene            3137..3628
                     /locus_tag="VGT87_001076"
     CDS             3137..3628
                     /locus_tag="VGT87_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEQ3813938.1"
                     /translation="MITQIRVDDRLIHGQVAVVWTKELNAPLLVVANDEAAKNEITQM
                     TLKMAVPNGMKLLIRSVEDSIKLFNDPRAKDKRIFVIVNSVKDACAIAKEVPDLEAVN
                     VANVGRFDKSDPASKVKVTPSLLLNPEEMAAAKELVSLPELDVFNQVLPSNTKVHLSQ
                     LVN"
     gene            3645..4454
                     /locus_tag="VGT87_001077"
     CDS             3645..4454
                     /locus_tag="VGT87_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEQ3813939.1"
                     /translation="MLVPATMAALAVLICFGGNYLTGQSMMERPLVVGLVTGLLLGDM
                     KVGILMGASLEALFLGNVNIGGVIAAEPVTATAMATTFTIISHIDQKAAMTLAVPIGM
                     LAAFVVMFLKNVFMNIFAPMVDKAAAANHQGKLVMLHYGTWIIYYLIIASISFIGILV
                     GSGPVNAFVEHIPQNLMNGLSAAGGLLPAVGFAMLMKLLWTNKLAVFYLLGFVLTAYL
                     KLPAVAVAALGAVICVISSQRDLELDAITRGAISKQTTFDSKESEEEDFFA"
     gene            4451..5278
                     /locus_tag="VGT87_001078"
     CDS             4451..5278
                     /locus_tag="VGT87_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEQ3813940.1"
                     /translation="MTSQDNLTKEDRKMLRSVFWRSWTMNASRTGATQYHAVGVIYTL
                     LPVINRFYKTDKDKAEALVRHTTWFNATMHINNFIMGLVASMEKKNSEDPDFDASAIT
                     AVKASLMGPISGVGDSFFWGILRVIAAGIGISLASAGSAMGAVVFLLLYNIPAFIIHY
                     YSLYGGYSVGAGFIKKLYESGGIKIVTKTSSMLGLMMVGSMTASNVKFKTILTVAAKG
                     AKEAASIQDYLDQLFIGIVPLMVTLAAFWLLRKKVNINWIMFGIMFLGIILGLLGIC"
     gene            5414..7063
                     /locus_tag="VGT87_001079"
     CDS             5414..7063
                     /locus_tag="VGT87_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ3813941.1"
                     /translation="MRGEQVEEHFKKQLQDDISRHFSYQSLMLSLLLIGLFIIFSLAP
                     QQLGLYRDINATATRYHRLISKQEALLDELGKNSLLPFLNKNLSTADLSKHYFHLRHS
                     SQTSPELLLFSPSQGLLFASNPHLGNVFSKSVYIQEVLRAAHPPKTLFKVAMDSEDGH
                     YLMIIKPMIDQNQLKGYAFLVMSGKDFLHPTKTLTSELVIADKLDNTFTFSNREFIAS
                     SLDKINSQYLHHYFVFQDNRAFITRKVALQGGLWLYMYRPLIPMVSVMLFSLISSAVI
                     FVILQRKSSGLANRIAAKNSRAINQMVRDMSAISRQEKRRIDLESQDEFQYLSDQINQ
                     MVERLQQLHDKTLDLETQKLLFEKRMLEAQFNPHFLYNTLETILITSHYDSALTEKIV
                     IQLTKLLRYSLTDSSKPVLLKDDLSVIESYLVINQVRFEELQYSINLSPDLDSLAVPK
                     LFLLPLIENAIKYGLKERHDVKINIACYYQDDHIIFSVRDNGSGIDAHHQKVIREQLE
                     AGESHHGLINSYRRLKYHFSEVSLVFDQGDKQFNVSYHVKE"
     gene            7067..7855
                     /locus_tag="VGT87_001080"
     CDS             7067..7855
                     /locus_tag="VGT87_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ3813942.1"
                     /translation="MYKLVIIEDEHIIRKWLRYAIDYKALDILVIGEAKDGKEGAVLI
                     KESQPDIVLTDINMPIMTAFDMFEATKDQTYAKIILSGYADFPNARSAIHYGVLEFLT
                     KPIEKAALWECLQTIIAKIEKQKGSNQKTDACVYIPLPQMTDQIPEVVKDILEWVHAH
                     FQDKISTSRLAHDLGYSESYIYQNIKKHLQMPLSDYINQYRINQAIQLMQQEPDLMVY
                     EIAQAVGIYDYRYFDRVFKKYLGQTVKAFKEEHFMKDTDRSKPC"
     gene            7849..8895
                     /locus_tag="VGT87_001081"
     CDS             7849..8895
                     /locus_tag="VGT87_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEQ3813943.1"
                     /translation="MLKLKRKWLLSFLLVIIILAIVNVAMCIFSSSKKDSAKELVILT
                     PNSQTILTGTIPAFEEKYGVKVRLIQGGTGQLIDQLGRKDKPLNADIFFGGNYTQFES
                     HKDLFESYVSPQVSTVISDYQLPSHRATPYTINGSVLIVNNELARGLHITSYEDLLQP
                     ALKGKIAFADPNSSSSAFSQLTNILLAKGGYTSSEAWEYVKRLLLSMNSIRATSSSDV
                     YESVAEGKMIVGLTYEDPCINLQKSGANVSIVYPDEGTVFVPSSVAIIKKAKAMKEAK
                     QFINFILSSDVQNAFGQSTSNRPIRRDAQTSHEMKPLDEIITLKEDYGYVTRHKKKIL
                     NTYNRLHEALERGK"
     gene            9010..10407
                     /locus_tag="VGT87_001082"
     CDS             9010..10407
                     /locus_tag="VGT87_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEQ3813944.1"
                     /translation="MTYQTIYPYTNEVLHTFDNMTDQGVADVLERAHLLYKKWRKEDH
                     LEERKAQLHQVANILRRDRDKYAEIMTKDMGKLFTEAQGEVDLCADIADYYADKADEF
                     LMSTPLETDSGQAYYLKQSTGVILAVEPWNFPYYQIMRVFAPNFIVGNPMVLKHASIC
                     PRSAQSFEELVLEAGAEAGSITNLFISYDQVSQVIADKRVVGVCLTGSERGGASIAEE
                     AGKNLKKTTLELGGDDAFIILDDADWDQLEKVLYFSRLYNAGQVCTSSKRFIVLDKDY
                     DRFKELLTKVFKTAKWGDPMDPETTLAPLSSAQVKADVLDQIKLALDHGAELVYGGEA
                     IDHPGHFVMPTIIAGLTKDNPIYYQEIFGPVGEIYKVSSEEEAIEVANDSNYGLGGTI
                     FSSNQEHAKAVAAKIETGMSFINSGWTSLPELPFGGIKHSGYGRELSELGFTSFVNEH
                     LIYIPNKTNNSNTKV"
     gene            10509..12305
                     /gene="uvrC"
                     /locus_tag="VGT87_001083"
     CDS             10509..12305
                     /gene="uvrC"
                     /locus_tag="VGT87_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEQ3813945.1"
                     /translation="MNELIKHKLELLPDSPGCYLHKDKEGTIIYVGKAKNLKKRVRSY
                     FRGSHDTKTELLVSEIVDFEYIVTESDTEALLLEINLIQKNMPKYNIKLKDDKSYPFL
                     KITNESFPRLVITRYIKKNDGLYFGPYPDSYTANEVKKLLDRIFPFKKCKNPINKVCF
                     YYHLGQCCAHTICHTDKAYWDRLIDDVKHFLNGKDDKIIEDLRSKMLAASEEMAFERA
                     AEYRDLISGIATMRTKQRVMSKDLQDRDIFGYYVDKGWMCVQVFFVRQGKLIQRDVNL
                     FPYYNDAEEDFLTYMGQFYQDKQHFIPKEVFIPEAIDEELVAAIVPTKIIKPKRGEKK
                     QLVALATKNARVSLQQKFDLLEKDIKKTSGAIENLGQLLRIDKPVRIEAFDNSNIQGT
                     SPVAAMVVFVDGKPSKKDYRKFKIKTVVGPDDYASMREVLFRRYSRVKKEGLQAPNLI
                     IVDGGVGQVNVAKDVIEKQLGLTIPVAGLQKNDKHQTHDLLFGNPLEVVPLPRRSEEF
                     FLLHRIQDEVHRFAVTFHRQVRRKNSFSSTLDHISGLGPKRKQLLLRHFKTITAIASA
                     TSEEIQALGIPKTVVEAIQQQITDNKNDRSSP"
     gene            12490..13092
                     /locus_tag="VGT87_001084"
     CDS             12490..13092
                     /locus_tag="VGT87_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEQ3813946.1"
                     /translation="MKFLELNKKRHAIKTFNDQPVDYEDLRTAIEIATLAPSANNIQP
                     WKFVVVQEKKAELAKGLPLANKVQVEQAQYVVALFSDTDLALRSRKIARIGVKSLPDD
                     LIGYYMETLPPRFAAFNEVQTGEYLAINAGIVAMNLVLSLTDQKIASNIILGFDKSTT
                     NEILDIDPRFRPELLITVGYSDEKPEPSYRLPVDEVIERR"
     gene            13217..14626
                     /gene="pepV"
                     /locus_tag="VGT87_001085"
     CDS             13217..14626
                     /gene="pepV"
                     /locus_tag="VGT87_001085"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEQ3813947.1"
                     /translation="MTTIDFKAEVDKRKEAMLADLVDLLRINSERDDQLADDKHPFGP
                     GPVKALEHFLAMAERDGYKTRNIDNYAGDFEFGQGDEVLGIFGHLDVVPAGSGWDTDP
                     YEPVIKDDRIYARGSSDDKGPTMACYYALKIIKELGLPVSKKVRFIVGTDEESGWGDM
                     DYYFAHNGLKNPDFGFSPDAEFPIINGEKGNITEYLHFAGDNKGAFVLHRFQGGLREN
                     MVPESATAVITAPHDLDVLEAALEQFLSEHGVKGSMKATDGKIEVTIIGKSAHGSTPE
                     AGVNGATLLAKFLNQFIFEGAAKDYLHVAGEVLHEDFAAEKLGLAYTDDRMGALSMNA
                     GVFTFDSQSADNTIALNFRYPKGTDAATLKAGLEKLPGLTKVSLSEHEHTPHYVPMDD
                     ELVATLLAVYEKQTGLKGYEQVIGGGTFGRLLERGVAFGAMFPGDENTMHQANEYMPL
                     ENIYRSAAIYAEAIYELIK"
BASE COUNT         4522 a         2669 c         3073 g         4373 t
ORIGIN      
        1 agaccggtcc tgaaggccca caaggcaaaa ccggcccggc aggtccagca ggtccacgtg
       61 gtgagcaagg cctgcaaggt gatcgcggtg aagttggacc aagaggtgaa caaggtcctg
      121 ctgggccagc tggaaaagat ggtgaaaaag gagaccaggg tccaatgggt ccacgtggtg
      181 accgcggcga agccggtccc gccggaaaag atggtgaacg tggtccagta ggtccagctg
      241 gcaaggacgg ccaagatggt aaagatggtc ttccaggcaa ggacggccaa aacggtaaag
      301 atggtctccc aggtaaagac ggcaaggacg gccaagatgg caaagatggt cttccaggca
      361 aggacggcca agatggcaag gacggtcaac caggtaaacc ggctcctaaa acaccagagg
      421 tccctcaaaa accagatacc gcaccacata ctccaaaaac ccctcagatc cctggccaat
      481 caaaagacgt gacacctgct cctcaaaacc cttctaatag aggtctaaac aaaccacaaa
      541 cacaaggtgg taatcagctc gcaaaaacac cagcagctca cgacacacac agacaattgc
      601 cagcaacagg cgaaacaacc aatccattct ttacagcagc tgctgtagct atcatgacga
      661 cagctggagt tgtagctgtt gcaaaacgtc aagaaaacaa ctaatcctct ttttgctacc
      721 taactgatgg aaagtaaaac tcagagagaa cctcaaggtt ctctcttttt ttgaatcaag
      781 atagtagctc taggtataac gtcttgtctg gggtgctgtg aacaaatcag ttactgctca
      841 cctctttttt tggtaaaata taagcactaa aacatcaaaa ggagttttcc atggaaacat
      901 ccagatgaat taaaacaacg cattggtgat ctttcttacg aggtcaccca gcatgcagca
      961 acagaatctc cctttacagg cgaatatgat aatttttttg agaagggtat ctatgtcgat
     1021 attgttagtg gtgaggtctt gttttcatca ctggataaat ttaattctgg ctgtggttgg
     1081 cctgcctttt caaaacccat tgaaaatcgt atggtcacaa accacgatga ctcttcttat
     1141 gggatgagac gagttgaggt taaaagccgt gaagcaggat cgcatttggg gcatgtcttt
     1201 agtgacggcc ccaaagaagc tggaggcttg cgttactgta tcaattcagc agctcttaaa
     1261 ttcattcctt atgagcagat ggaaaaagaa ggctatgccc agtggctcac attgtttgat
     1321 gagacataac agggtttcaa aatgactgaa aaagaagcgt cagtgattcc cataagcact
     1381 ggattggtga ggcgtacggg tcaattttac aagcctttgt aaaaggtcta taataaggaa
     1441 tgaaaaaagg aggttagtat gtcaactcac ttaagaaaac ttccaggcct gttactttgt
     1501 ctgcttttag ctcttccagc ctggtgttta gggcgcttgt ttcctattat tggagctcct
     1561 gtttttgcta ttcttttagg aatgttgtta gccttgtttt atgaacatcg tgacaagact
     1621 aaagagggaa ttagttttac atccaagtgt attttgcaaa cagcagtggt tttgcttggt
     1681 tttggattaa acctaaccca agttatggca gtaggcatgc agtctttacc cattatcatt
     1741 tcaactattg cgacagctct tttggtagct tatggctcac agaaatggct gcgcttagat
     1801 gtcaatacag ccaccttggt gggtgtaggg tcttccattt gtggggggtc tgctattgca
     1861 gcgacggctc ctgtcattaa ggcaaaggat gatgaggttg ctaaggcaat ttcagtcatt
     1921 tttctcttta atatgttagc agctttgcta tttccaagtt taggacaatt actgggctta
     1981 tctaatgaag gttttgctat ttttgctggg acagctgtta acgatacctc ttcagtaacc
     2041 gcgacagcaa cagcttggga tgccattcac cattccaata cactagatgg cgcaaccatt
     2101 gttaaattga ctcgcacctt ggctattctt ccaattactt taggcttatc cctttaccga
     2161 gcgaaaaaag agcacgacat cgttacagaa gaaaacttta gccttaggaa gtcattccct
     2221 cgtttcatcc tcttcttttt attagcttct ctcattacaa cgctgatgac cagtttggga
     2281 gtttctgccg atagtttcca ttccctaaaa accttatcaa aattctttat cgtgatggct
     2341 atggcagcga ttggtttaaa cacaaacttg gttaaattaa tcaagacggg cggtcaggct
     2401 atccttttag gagctatttg ctgggtagct atcacccttg tcagtttagc catgcaatta
     2461 agtttgggca tttggtaatc agacacgaga aatcagtttg actgatttct ttttttgtat
     2521 cttatttgtg tagaattgtc tacattttaa agagaatagt gcattttaaa aacgttttca
     2581 tttgataaaa taaacctatc gttaaaccag ttcagtaaag acaactggtt gagataagca
     2641 attaccgttt attcagcggt ttttgttaat agaggaggat aggaatgaaa cgaaaatttc
     2701 tcattgggag tcacggcaga ctagccagtg gtttgcaaag ctctattgac attttagcag
     2761 gtatgggaca ggcacttgaa accattgatg cttatgttga tgatagtgac tacactagcc
     2821 agattgatga ctttatcgct ggggtagcag cagatgagca gggccttatt tttactgatt
     2881 tgttaggcgg cagtgtgaat cagaaaatgg taaccgctgt aatgaacagt ggcaaagaca
     2941 atatcttttt aatcacaaac agtaacttag ccactttatt gtcgttggta tttttaaaac
     3001 caggcgaagc gcttactaaa gatgaaattg taaccgttat taatgaatcg caagttcagt
     3061 tagtggattt ggtccctgag acaaattcag aagatgactt ttttgattaa tatcttaata
     3121 tctaggagga agacaaatga ttacccaaat tcgtgttgac gaccgtttga ttcacgggca
     3181 agtggcagtt gtatggacca aagagttaaa tgcgccgctt ttagtagtgg ctaatgatga
     3241 agctgcaaaa aatgaaatca cacaaatgac gcttaaaatg gctgtgccaa acggcatgaa
     3301 actgcttatt cgttcagttg aagactcgat taagctcttt aatgatccac gcgccaaaga
     3361 caagcgtatc tttgtgattg tcaattctgt taaagatgct tgtgccattg ctaaagaagt
     3421 tccagactta gaagctgtta atgtggctaa tgtggggcgc tttgataagt cagacccagc
     3481 aagcaaagtc aaagtgacac caagtctttt acttaaccca gaagaaatgg cagccgcaaa
     3541 ggaattagtc agtttaccag aacttgatgt ctttaaccaa gtcttaccat caaataccaa
     3601 ggtccatttg agccaattag tcaactaaag agaggtaaaa aagtatgtta gtaccagcaa
     3661 caatggcagc tttagctgta ttgatttgtt ttgggggaaa ttatttaacc ggtcaaagca
     3721 tgatggaaag acccctagta gttggactgg tcacaggtct tcttttagga gatatgaagg
     3781 tcggtatctt aatgggggcc tctctagaag ctcttttcct aggaaatgtg aatattgggg
     3841 gtgttattgc agctgagcct gtaacagcga ctgccatggc aacaaccttt accattattt
     3901 cacatattga ccaaaaagca gccatgaccc tagctgttcc aattggaatg ttagcagcct
     3961 ttgtggttat gtttttgaaa aacgtcttca tgaatatctt tgctccaatg gttgataaag
     4021 ctgcagcagc taaccatcaa ggtaaactag tgatgctcca ctatggcact tggattatct
     4081 attacttaat cattgcctct atttctttca ttggtatttt agtaggtagt ggacctgtta
     4141 atgcctttgt ggaacacatt cctcaaaatc tcatgaatgg cttgagtgct gcgggaggcc
     4201 tccttccagc tgtcggtttt gccatgctga tgaaattatt gtggaccaac aaattagctg
     4261 ttttttacct attgggcttt gtcttaacag cttatttgaa attaccagcg gttgcagttg
     4321 ctgccctagg agcagttatt tgcgtcatta gttcccaacg tgatctagaa ttagacgcaa
     4381 ttactagagg tgcaattagt aaacaaacaa cctttgactc taaagaatca gaagaggagg
     4441 acttcttcgc atgacatcac aagataattt gactaaagaa gaccgtaaaa tgcttcgctc
     4501 agtcttttgg cgctcatgga cgatgaatgc cagtcgtact ggggcaacac aataccacgc
     4561 agtaggggtt atttacactt tactaccagt catcaatcgt ttttataaga ctgataagga
     4621 taaagcagag gcactggtcc gtcatacaac atggttcaat gccaccatgc acatcaataa
     4681 ctttatcatg ggtcttgtgg catcgatgga aaagaaaaac agtgaagatc ctgattttga
     4741 tgctagtgct attacggcgg taaaagcatc tctaatggga cctatttccg gcgttggaga
     4801 ttccttcttc tggggaattt taagggttat tgcggcaggt atcgggattt ccttggcaag
     4861 tgcaggctca gccatgggag ctgttgtctt cttactcttg tacaatattc ctgcttttat
     4921 tattcactac tatagccttt atggtggtta ttctgtaggt gcaggattta tcaaaaaact
     4981 ttacgaatct ggtggtataa aaatcgtcac taaaacctca agtatgcttg ggttaatgat
     5041 ggtaggctca atgacggctt caaatgtaaa atttaaaacg attcttacag ttgctgcgaa
     5101 aggtgccaaa gaagcagctt ctattcaaga ttaccttgac caattgttta ttggtattgt
     5161 accattaatg gtaacactag cagctttttg gcttctacga aagaaagtga atatcaactg
     5221 gattatgttt ggtatcatgt tccttggcat tatccttgga cttttaggta tttgctaata
     5281 atctcctagc aataaaggta agtaagacaa gttttcttgt tttgcttacc tttttccttt
     5341 taagatgatg agacttgctt tgatgaaaaa cgtagtagca ttaagaaatt tgttataatg
     5401 aaaggagctt aagatgcgag gtgaacaggt ggaagaacat tttaaaaaac aattacaaga
     5461 tgatatttct agacattttt cgtaccaatc gttaatgtta tcattgttgc taattggtct
     5521 ttttattatt ttttcattag cgccacaaca attaggcctc tatcgtgata tcaatgccac
     5581 tgcaactcgt tatcaccgtt tgattagcaa acaagaagcc ttgctggatg aattagggaa
     5641 aaatagctta ctaccttttt taaataaaaa cctcagcact gctgatttaa gcaagcacta
     5701 ttttcatttg cgccatagca gccaaacgtc accagaactt ttactatttt cacctagtca
     5761 aggcctttta tttgctagta acccacattt aggaaatgtt tttagtaaat ctgtttatat
     5821 tcaagaagtc ttgagagcag ctcatcctcc aaaaaccttg tttaaagttg ctatggatag
     5881 tgaagatggt cactacttga tgatcattaa gccaatgatt gatcaaaacc aactaaaggg
     5941 atatgctttt ttagtgatga gtggcaaaga ttttcttcat cctacaaaaa cattgacgtc
     6001 agagttggtc attgctgata agctggacaa cacgtttacg ttttccaatc gtgagtttat
     6061 agcatctagt ctagataaga tcaatagcca gtatttacac cattattttg tttttcaaga
     6121 taatcgagcc tttatcacaa gaaaggttgc cttacaagga ggtctttggc tttacatgta
     6181 taggcccttg attccaatgg tatcggtgat gttattttca cttatttcat cagctgttat
     6241 ctttgtgatc ttgcaacgta aatcaagtgg tttagctaac cgaattgcag ctaaaaattc
     6301 aagagcaatc aatcaaatgg ttagagatat gagtgcgatc tctcggcaag aaaaaagacg
     6361 tattgacctt gagagtcaag atgaatttca atatttatct gatcaaatca atcaaatggt
     6421 agagcgattg caacagttac atgataaaac gttagatttg gaaactcaaa aattattatt
     6481 tgaaaaacgg atgttagaag ctcaattcaa tccgcatttc ctctacaata cgctagaaac
     6541 cattttgatt acgagccatt atgattctgc cctaacagaa aaaattgtta tccaattgac
     6601 aaaattgttg cggtatagtc tcacggattc tagcaaacct gtcttgctca aagatgattt
     6661 aagtgtcatc gagtcttatt tagtgattaa tcaggtgcgg tttgaagaat tgcaatatag
     6721 cattaaccta tccccagacc ttgatagtct tgcagtgcct aaattgttct tgttaccttt
     6781 gatagaaaat gccatcaaat atggcttgaa agaacgacac gatgtcaaga ttaacattgc
     6841 ttgttattat caagatgacc acattatttt ttctgtgcga gataatggtt cgggaattga
     6901 tgctcatcac caaaaagtga ttcgagagca attggaagct ggagagtcac accatgggtt
     6961 aattaactct tatcgtcggc ttaagtatca tttttcagaa gtatccttgg tttttgatca
     7021 aggtgataaa cagtttaatg ttagctacca tgttaaggag tagtctatgt acaaattagt
     7081 cattattgaa gatgaacata ttattaggaa atggttgcga tacgctattg attataaagc
     7141 tttagatatt ttggtcattg gcgaggctaa agatggcaaa gaaggagctg ttttaatcaa
     7201 agagagtcaa ccagatatcg ttttaaccga tattaacatg cccatcatga cagcttttga
     7261 catgtttgaa gcaacaaaag atcaaactta cgccaaaatt attttatctg gctatgccga
     7321 ttttccaaat gcaaggtctg ccatacacta cggtgtactt gaatttttaa caaaaccgat
     7381 tgaaaaagca gctctttggg aatgtcttca aaccattatt gctaaaatag aaaagcaaaa
     7441 ggggtctaat caaaaaacag atgcttgcgt ctatattccc ttaccccaga tgactgatca
     7501 gattcctgaa gtagtcaaag atattttgga gtgggtacat gctcattttc aagacaagat
     7561 cagcacgagc cgcctagctc atgacttagg gtatagtgag agttatatct atcaaaatat
     7621 caaaaagcat cttcagatgc ctttaagtga ttacattaat caatatcgga ttaatcaggc
     7681 aattcaattg atgcaacaag aaccagattt aatggtatat gaaatagcac aagctgtagg
     7741 tatttatgac tatcgctatt ttgatagggt ttttaaaaag tatctaggac aaactgtaaa
     7801 ggcttttaaa gaagagcatt ttatgaaaga taccgatagg agtaagccat gttaaaactc
     7861 aaacgcaaat ggctattaag ctttttgtta gtaataatta ttttagcaat cgttaacgtt
     7921 gcaatgtgta tattctcatc gtcaaaaaaa gatagtgcta aagaattagt tatcttgact
     7981 cctaatagcc aaactatttt aacagggact attccagcct ttgaggaaaa gtatggggtt
     8041 aaagtaagat taatccaagg tgggacgggc caacttattg atcaattagg tcgaaaagat
     8101 aagccattaa acgctgatat tttctttggt ggcaattaca ctcaatttga aagccataaa
     8161 gatttatttg aatcctatgt ttctccgcag gtttctactg tcatttcaga ttaccaattg
     8221 cctagtcatc gtgcaacccc atatacgatc aatggcagtg tactgattgt taataacgaa
     8281 ttagcaagag gacttcatat taccagttat gaggatttgc tacaaccagc tttaaaaggc
     8341 aaaattgctt ttgctgaccc aaatagctca tctagcgctt tttcacaatt aacaaacatc
     8401 ttactggcta aaggaggcta tacgagctca gaagcttggg agtatgtgaa acgtttatta
     8461 ctgtctatga attctatcag agcaacaagt tcttcagatg tttacgaatc tgttgcagaa
     8521 ggtaaaatga ttgttggttt gacctatgaa gatccttgca tcaatctgca aaaaagcggt
     8581 gcaaacgttt cgattgttta tcctgatgag gggacagtat ttgttccgtc atcagtcgct
     8641 attatcaaaa aagcaaaggc aatgaaagaa gctaagcaat ttattaattt tattttatca
     8701 tcagatgtac aaaatgcctt tgggcagtca actagtaata gacctatccg acgagatgct
     8761 caaacaagtc atgaaatgaa accattagat gaaattatta ctttaaaaga agattacgga
     8821 tatgttacta gacataagaa aaaaatattg aatacctata atcgactaca tgaagcatta
     8881 gaaagaggaa agtagactct aggcttgttg ttgctatact agtagtgtta caaaatagcg
     8941 tagtcatttt ttgaaatcga tttcataatc tggtagacta aggttatcac gaaaaggagg
     9001 ctatatgtga tgacttatca aactatttac ccttatacaa atgaagtcct tcatacattt
     9061 gataatatga ccgaccaagg tgtggcagat gtccttgaaa gagctcacct tctctataaa
     9121 aaatggcgca aggaagatca tttagaagaa cgtaaagccc agcttcatca agtggctaat
     9181 attcttcgcc gtgaccgcga taaatatgct gaaatcatga ccaaagacat gggaaaactg
     9241 ttcacagaag cccaaggtga agtagacctt tgtgccgata ttgctgatta ctacgctgat
     9301 aaggcagatg aatttttgat gtctacgcca ctagaaacag attcaggtca agcttactat
     9361 ttgaaacaat caactggtgt tatcctagct gttgagcctt ggaatttccc ttactatcaa
     9421 atcatgcggg tttttgcgcc aaactttatt gttgggaacc caatggtttt aaaacacgcg
     9481 tccatttgcc cacgctctgc ccaatctttc gaagaattag ttcttgaagc tggtgctgaa
     9541 gcaggtagta ttaccaattt gtttatttct tatgaccaag tatcacaagt gattgctgat
     9601 aaacgtgtgg ttggtgtctg cttgacaggt tccgaacgtg gaggagctag cattgcagaa
     9661 gaagctggta aaaacttgaa aaaaacaacc cttgaattgg gtggagatga tgcctttatt
     9721 atccttgatg acgcggattg ggatcaatta gagaaggtgc tttacttctc tcgtctctac
     9781 aatgcaggac aagtatgtac ctcatcgaaa cgttttatcg tgcttgacaa agactatgac
     9841 cgtttcaaag aattattaac aaaagtcttc aaaacagcta aatggggtga cccaatggat
     9901 cccgaaacga cgttggcacc attatcatca gctcaagtta aagcagatgt tcttgaccaa
     9961 attaagttgg ccttggatca tggtgcggag ttggtttatg gtggtgaagc gattgatcat
    10021 ccaggtcatt ttgtcatgcc aaccattatt gctggcctca caaaagacaa ccctatttac
    10081 tatcaagaaa tctttggccc agtcggtgaa atctacaaag tgtcttctga agaagaagcg
    10141 attgaagtgg cgaatgattc taactatggc cttggaggta ctatctttag tagcaatcaa
    10201 gaacatgcga aagccgtagc tgctaaaatt gaaactggta tgtcttttat caactcaggt
    10261 tggacaagtc ttccagaact cccatttggt gggattaaac attcaggcta tggtcgtgaa
    10321 ttgagtgagc ttggctttac atcttttgtg aatgaacacc ttatctatat tccgaataaa
    10381 acgaataata gcaatactaa agtttgatct agattgttaa aggagatctg ctcgccttaa
    10441 taaacaatca aggcgttaca ctcttctttt ttaaagtcaa aattttaaaa atttgataaa
    10501 ctagtaacat gaacgaactg attaaacata aactagaact cttaccagac agccctggct
    10561 gttacttaca taaagataaa gaaggaacta ttatctatgt cggtaaggca aagaatttga
    10621 aaaaacgcgt ccggtcctat tttcgtggga gccatgatac caaaacagag ttactagtat
    10681 ctgagattgt agatttcgaa tatattgtaa cagagtcaga taccgaagcg cttttgttgg
    10741 aaattaactt gattcaaaaa aatatgccta agtacaacat caagttaaaa gatgataaat
    10801 cttatccctt tctcaaaatc acaaatgagt cttttcctcg gttagtgatt acaagatata
    10861 ttaagaaaaa tgatggattg tactttgggc cctacccaga ttcttacact gctaatgaag
    10921 ttaaaaagtt attagatagg atttttccat ttaaaaagtg taaaaatcct atcaataagg
    10981 tctgttttta ttaccattta ggccagtgct gtgctcatac catttgtcat acagacaagg
    11041 cctattggga tcgtttaatt gatgatgtta agcacttttt aaatggtaaa gatgacaaaa
    11101 ttattgaaga tcttcgttct aagatgttag cagcttctga agaaatggca tttgaacgtg
    11161 ccgctgaata tcgagatttg atttcaggta ttgccaccat gcgcaccaag cagcgggtga
    11221 tgagcaagga tttgcaagat agagatatct ttggctatta tgttgataag ggatggatgt
    11281 gtgtccaagt tttctttgtg cgtcaaggga aattaattca gcgtgatgtt aacctttttc
    11341 cctattataa cgatgcagaa gaagatttct taacttatat ggggcaattt taccaagata
    11401 aacagcattt catccctaaa gaagtcttta ttccagaagc tattgatgaa gaactggtag
    11461 ccgctattgt gccaaccaaa atcatcaaac ccaaacgcgg tgaaaaaaaa caactggtag
    11521 ctttggctac taagaatgct cgtgttagtc tgcaacaaaa atttgattta ttagaaaaag
    11581 atatcaaaaa aacgagtggt gctatcgaaa atttaggtca gttacttagg attgacaagc
    11641 ctgtgcgcat tgaagccttt gataattcta acattcaagg gacaagtcca gttgcggcta
    11701 tggttgtttt tgttgatggc aaacctagta agaaagatta tcgtaaattt aagattaaaa
    11761 cagtggtggg accagatgat tatgccagta tgagagaagt gctttttcga aggtacagtc
    11821 gggtgaaaaa ggagggactg caagctccta acttgattat tgttgatgga ggcgttggac
    11881 aagtcaatgt ggccaaagat gtgatagaaa aacaacttgg actcactatt cctgttgcgg
    11941 gacttcaaaa aaacgataag caccagaccc atgatttgct ttttggaaac ccgcttgaag
    12001 ttgtaccgtt gccgcgccgt tctgaagagt ttttcttact gcatcggatt caagacgaag
    12061 tgcatcgctt tgcagttaca tttcatagac aagtgcgacg taaaaattct ttttcatcaa
    12121 cattagatca tatttcaggg cttggcccaa agcgaaaaca gttattacta agacacttta
    12181 aaaccataac agcgattgca tcagctacct ctgaggagat tcaagcttta ggtataccta
    12241 aaacagtagt tgaagcaata cagcaacaga taacagacaa taaaaatgat agatcgtctc
    12301 cttaaataag tgttatgttt tcagtactga tgaggtaaaa aggcgctact tgttacgcac
    12361 gacatttcag ttcgttattt tgaaactttc agaaagttgc gaagtgtagt gagtttatga
    12421 ttttaaaaac tattttatgg taaaataaaa tggtattaaa aaaaacagat ttagatagga
    12481 agttttgaaa tgaaatttct agagttaaac aaaaaacgcc atgccattaa gacctttaat
    12541 gaccagccag ttgactacga ggatttgcga acagccattg agattgcaac cttagcacct
    12601 agtgccaata acattcaacc ttggaaattt gtagtcgttc aagagaaaaa agctgagcta
    12661 gcaaaagggt tacctctagc aaataaagtg caggttgaac aggctcaata tgtagtggcg
    12721 ctattttcag acactgattt agctttacgt tctcgtaaaa ttgcccgtat cggtgtcaaa
    12781 tcattaccag acgacttaat tggttactat atggagactt tgccaccacg ttttgctgct
    12841 tttaatgagg ttcaaaccgg tgagtactta gctattaatg caggcattgt ggctatgaac
    12901 ttggtgttat ccttgacgga tcaaaagatt gcttccaata ttatccttgg ttttgataag
    12961 tcaacgacca atgagatttt agacatcgat ccgcgttttc gtccagaatt attgattact
    13021 gttggctata gtgacgagaa accagaacca agttatcgtt tgcctgttga tgaagtcatc
    13081 gaaagaagat agagtcagga caattagtta aatgtaatgt ccagatgcgc tactgagcga
    13141 ccactattgt tagactgata ctattttggc ttattttaag ttaaacatta tccctaaaac
    13201 gaataaggag agtataatga caaccattga ttttaaagca gaagttgaca aacgtaaaga
    13261 agctatgcta gcagatttag ttgacctatt acgcattaat tcagaacgcg atgaccaatt
    13321 agctgatgat aaacacccat ttggcccagg tccagttaag gctttggagc acttcctcgc
    13381 aatggctgag cgtgatggct acaaaacgcg taatattgat aactatgcag gtgactttga
    13441 atttggtcaa ggtgatgaag tactaggaat ctttggtcac cttgatgttg tgccagctgg
    13501 tagcggttgg gatacagacc cttatgagcc agtgattaaa gacgatcgca tctatgcgcg
    13561 tgggtcttcg gatgataagg gaccaaccat ggcgtgctac tacgctctga aaatcattaa
    13621 agaattgggt cttcctgtgt ctaaaaaagt tcgtttcatt gttggtaccg atgaagaatc
    13681 tggctggggg gatatggatt attactttgc tcataatggc ttgaaaaatc cagattttgg
    13741 tttctcacca gatgctgaat ttccaattat caatggtgag aaaggaaata tcaccgaata
    13801 cctccatttc gctggtgaca acaagggcgc ttttgtgctt catcgcttcc aaggtgggtt
    13861 gcgtgaaaac atggtaccag aatctgcaac agctgtcatt acggcaccgc atgaccttga
    13921 tgtgctagaa gcagctttgg agcaattttt atcagaacat ggtgttaaag gttctatgaa
    13981 ggctactgat ggtaaaattg aagtaactat tattggtaag tctgctcacg ggtcgactcc
    14041 agaagcgggt gttaatggtg ctaccttgtt ggctaaattc ctgaatcaat ttatctttga
    14101 aggggctgct aaggattatt tacatgtggc aggtgaggtg cttcatgaag actttgcggc
    14161 agaaaaacta ggccttgctt atactgatga ccgtatggga gccctgagca tgaacgcagg
    14221 tgtcttcacc tttgatagtc aatcagctga caataccatc gccttgaatt tccgctatcc
    14281 caaaggaact gatgcggcaa ccttaaaagc aggattggaa aaattaccag gattaactaa
    14341 ggtaagcctt tctgagcatg aacatacacc tcattatgtg ccaatggatg atgaattagt
    14401 agctacctta cttgctgttt acgaaaaaca aacaggtctt aaaggctacg aacaagttat
    14461 cggcggagga acctttggtc gtttgctaga acgtggtgtg gcctttggag cgatgttccc
    14521 aggcgatgaa aacactatgc accaagctaa tgaatatatg cctttagaaa atatttatcg
    14581 ctcagcagct atttatgctg aagcaatcta cgaattaatt aaataagatg cttatga
//
LOCUS       DAUCLR010000042        14561 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.042, whole genome shotgun sequence.
ACCESSION   DAUCLR010000042 DAUCLR010000000
VERSION     DAUCLR010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14561
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.042"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            92..1084
                     /locus_tag="VGT87_001086"
     CDS             92..1084
                     /locus_tag="VGT87_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEQ3813948.1"
                     /translation="MVMIEVSHLQKNFSKTIKEPGLKGALKSFVHPQREIFEAVKDLS
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGYCRINGKIPQDNRQDYVRDIGAV
                     FGQRTQLWWDLALQETYVVLKEIYDVPEKAFRKRMDFLNEVLDLNEFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIIMIDKGQEIFDGTVTQLKQTFGKMKSLSFELEAGQEEVVSQFLGLPDIT
                     VERHELTLEIQYDSSRYQTADIIQKTIADFAVRDLKMTDVDIEDIVRRFYRKEL"
     gene            1086..1904
                     /locus_tag="VGT87_001087"
     CDS             1086..1904
                     /locus_tag="VGT87_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEQ3813949.1"
                     /translation="MWSFWKRYSPFLSAGIQELITYRVNFFLYRIGDVMGAFVAYYLW
                     KAVFDSSKQSLINGFTLSDMTFYIIMSFVTTLLTKSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFMEIGFRWIVLMSVGFPFLVVLSGIKVMAGLSILQVLASSCLYLVSLLLA
                     FLINFYFNICFGFSAFVFKNLWGSNLLKNALVAFMSGSLIPLAFFPKMVSIVLSFLPL
                     SSLVYTPVMIVIGKYSLSQIMVALSLQIFWLLVMVVLSQVIWKKVQYHLTIQGG"
     gene            1906..2691
                     /locus_tag="VGT87_001088"
     CDS             1906..2691
                     /locus_tag="VGT87_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ3813950.1"
                     /translation="MAKLRRMHAIFIKQYLKQIMEYKVDFVVGVLGVFLTQGLNLLFL
                     SVLFQHIPSLEGWTFEQIAFIYGFSLIPKGIDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PISPLFHVLVETFQVDALGELLVGVILLVTTAGSIVWTLPKVLLFILVIPFATLIYTS
                     LKIATASISFWTKQSGAVIYIFYMFNDFSKYPMSIYHSFLRWLISFIIPFAFTAYYPA
                     SYFLTGQHLLFNIGGLVVVSLLVLALSLKLWKWGLDAYESAGS"
     gene            2892..3041
                     /locus_tag="VGT87_001089"
     CDS             2892..3041
                     /locus_tag="VGT87_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase"
                     /protein_id="HEQ3813951.1"
                     /translation="MQLLDNIYGTLRSFAFMRPVKSSNDTSLADALQADTDMAFILVR
                     GINYQ"
     gene            3436..4584
                     /locus_tag="VGT87_001090"
     CDS             3436..4584
                     /locus_tag="VGT87_001090"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEQ3813952.1"
                     /translation="MTDVYIAAGLRTPIGLVGKQFAKEQPEILGAKLINALQNKYPVP
                     IDQVICGNAVGTGGNIGRLMTLYSHLGESVSALTVDMQCASAGAALSVGYAKIKAGMA
                     SNLLVGGIESSSLQPESVYASADWRQGAYKVAQFSPDSISPFAMIEGAERVAREHGFT
                     KEYLNHWTLRSHQKASYCQEQALLADLILDLPGASDQGIRPRLSSKVLSKVPPILGED
                     HVISAANACLTHDAAAFLQLSSQPSAFKLIDVVEVAGDPQRSPLMVIKASQVLLEKHG
                     LGMADMTAIEWNEAFAVIDGLFETHYPDLLDRYNIFGGALAYGHPYGASAAIIILHLM
                     RALEIKNGHYGIAAIAAAGGQGFAVLLKYHKEFSHADKARILGKAMPK"
     gene            4541..5788
                     /locus_tag="VGT87_001091"
     CDS             4541..5788
                     /locus_tag="VGT87_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEQ3813953.1"
                     /translation="MLTKLEYWAKQCPNKKAIVADQISLTYQELWQAVLIKDQTIKDS
                     VPYIISHSRYLNQLLSFLRGLKEGSCPIILHPNISGTFQQQIKHVDGELLKKADFAVL
                     SSGTTGKAKLFWRCLSTWTRLFDYQNKVFGMTGNSCLFLHGSFSFTGNLNLALAQLWA
                     GGCLVLSQKLSLKTWLSLWQAKKVSHLYLLPTYLNRLLPYLTKNNMTATHLLTSSQMI
                     SQELLRHYYKKFPQLEIVIFYGASELSFITWCNGRAAVKINGLVGQPFPDVSISFKDK
                     EIFVETPYSVEGMSQPYSVSDLGKMSPAGLILEGRQDDWVNQRGVKCHLPSLVELAHQ
                     APNVKEAHALKIGKGENETLILVLVLTKKDCLAPIKDFLALYLNSGQLPKYYLVIDCL
                     PLKDNGKINREVLLNKIPKQWLS"
     gene            5844..6878
                     /locus_tag="VGT87_001092"
     CDS             5844..6878
                     /locus_tag="VGT87_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HEQ3813954.1"
                     /translation="MCYNMVKKDEEKAMKQAILDRYQALKCYQNAGLSNQAFRAIAKE
                     PIIDNRLGSPTFWVIWPIEKENQSAKQLLTFLLDLVEMPFELSGQLHETQTLLTRFHP
                     SLLPDHMFWKELASLVDQAFPGKTLSQAGELEKRLHQFRYIISSQQAQSIRNHYKMIE
                     MTDAQALALFLRSKKGPCLWRQAPDYTLMDSARLHNKLRFEDNKVIFPSQEVSYNIKV
                     LLWFHTEFILDSTGFFLNEVDAEVVTEKGIVNGASFNYGTDGPRHWDLDVDPISRHDP
                     QFRRDTLKGFRTPKRVFRQWFRAQKDDFMFSYFNAKGLFAYHNKSSFARVKKSAKQFK
                     RQIHPIKGWF"
     gene            7040..7750
                     /locus_tag="VGT87_001093"
     CDS             7040..7750
                     /locus_tag="VGT87_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019321978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ3813955.1"
                     /translation="MKKILIVDDEKPISDIIKFNLTKEGYDIVTAFDGREAVTIFEEE
                     KPDLIILDLMLPELDGLEVAKEIRKTSHVPIIMLSAKDSEFDKVIGLEIGADDYVTKP
                     FSNRELLARVKAHLRRTETIETAVAEENASSGTQELTIGNLQILPDAFVAKKHGQEVE
                     LTHREFELLHHLANHMGQVMTREHLLEIVWGYDYFGDVRTVDVTVRRLREKIEDTPSR
                     PEYILTRRGVGYYMKSYD"
     gene            7743..9095
                     /gene="vicK"
                     /locus_tag="VGT87_001094"
     CDS             7743..9095
                     /gene="vicK"
                     /locus_tag="VGT87_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEQ3813956.1"
                     /translation="MTRDIIGNLSTFELAILILLVFVAFYFIHLAVRDYRNARIIRMM
                     SHKIRDLINGRYTDIIDEKADIELMELSDQLNDLSDVFRLTHENLAQEKNRLASILAY
                     MSDGVLATDRSGKIIMINETARKQLNLSKEEALKKNITDLLEGDTSYTYRDLVSKTPV
                     VTVNSRNDMGEFVSLRLRFALNRRESGFISGLVVVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALKEDIAPSFIKVSLDETNRMMRMISDLLNLSRIDNQVT
                     QLAVEMTNFTAFITSILNRFDLVKNQHTGTGKVYEIVRDYPITSVWLEIDNDKMTQVI
                     ENILNNAIKYSPDGGKITVRMKTTDTQLIISISDQGLGIPKTDLPLIFDRFYRVDKAR
                     SRAQGGTGLGLAIAKEIIKQHHGFIWAKSDYGKGSTFTIVLPYEKDAAIYEEWEEDVD
                     "
     gene            9099..9908
                     /locus_tag="VGT87_001095"
     CDS             9099..9908
                     /locus_tag="VGT87_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEQ3813957.1"
                     /translation="MNESGFKYSILASGSTGNCFYLETPKKRLLIDAGLTGKKITSLL
                     AEIDRKPEDLDAILITHEHSDHIKGVGVMARKYHLDIYANEKTWQLMDECNMLGKLDA
                     SQKHIFQRDKVLTFGDVDIESFGVSHDAIDPQFYRIMKDNKSFVMLTDTGYVSDRMTG
                     IIENADGYLIESNHDIEILRSGSYPWSLKQRILSDLGHLSNEDGAGAMIRSLGYNTKK
                     IYLGHLSKENNIKELAHMTMVNQLAMADLAVGTDFTVHDTSPDTACPLTDI"
     gene            10362..11054
                     /locus_tag="VGT87_001096"
     CDS             10362..11054
                     /locus_tag="VGT87_001096"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEQ3813958.1"
                     /translation="MKQLEELLSTSFDIQFNDLTLLETAFTHTSYANEHRLLNVSHNE
                     RLEFLGDAVLQLIISEYLFAKYPKKTEGDMSKLRSMIVREESLAGFSRFCSFDAYIKL
                     GKGEEKSGGRRRDTILGDLFEAFLGALLLDKGIDAVRRFLKQVMIPQVEKGNFERVKD
                     YKTCLQEFLQTKGDVAIDYQVISEKGPAHAKQFEVSIVVNGAVLSKGLGKSKKLAEQD
                     AAKNALAQLSEV"
     gene            11055..>14561
                     /gene="smc"
                     /locus_tag="VGT87_001097"
     CDS             11055..>14561
                     /gene="smc"
                     /locus_tag="VGT87_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEQ3813959.1"
                     /translation="MFLKEIELEGFKSFADKTKIEFDKGVTAVVGPNGSGKSNITESL
                     RWALGESSAKNLRGGKMPDVIFAGTQNRNPLNYAKVAVVLDNSDHFIKTAKKEIRVER
                     HIYRNGDSDYLIDGRKVRLRDIHDLFMDTGLGRDSFSIISQGRVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRKKETQIKLNQTQDNLDRLEDIIYELDTQLAPLEKQAKVAKQFLEL
                     DANRKQLQLDILVKDIDIAQERQTKDTEALAALQQDLASYYAKRQSMEEDYQKFKQKK
                     QVLSQESDQTQTTLLELTKLIADLEKQIELVKLESGQEAEKKAEAKKHLEQLQEQLDG
                     FQAEEKQRTEQLLHIDQQLCDVKQQLNELSNALERFSSDPDQLMETLREEFVLLMQKE
                     AALSNQLTALKAHLDKEKQARQHKAQEYQLLVTKLDQLNDESQKAQAHYKAQKEQVEM
                     LLQNYQEGDKRVQELERDYQLNQERLFDLLDQKKGKEARKASLESIQKSHSQFYAGVR
                     AVLQSQKKLGGIIGAVSEHLSFDSDYQTALEIALGANSQHIIVTDEAAAKRAIAYLKK
                     NRQGRATFLPLTTIKARSLSEHYHRQLATCEGYLGTAESLVRYDDSLSAIIQNLLSST
                     AIFETIDQANIAARLLGYKVRIVTLDGTELRPGGSFSGGANRQSNTTFIKPELEQISE
                     ELTRLVEQLKITEKEVAALQSDLIAKKEELTQLKLAGDQARLAEQRAQMAYQQLQEKQ
                     EDSKALLAALDQSQTTHSDESLLAEQARIEEALTAIAKKKSALTCDIDDIKENKDLVR
                     QKTQNIHQALSQARLQERDLLNEKKFEQANQSRLRTQLKQCQQNILKLESILNNNVSQ
                     DSIQRLPQWQKQLQDATEHKSGAQKRLVQLRFEIEDYEARLEETAEKITKESEKNDTF
                     IRRQTKLETHLEQVANRLRAYAKSLSEDFQMTLADAKEVTNSIDHLESAKEKLHHLQK
                     TIRALGPINSDAITQYEEVHERLTFLTSQKTDLTKAKNLLLETINSMDSEVKARFKMT
                     FEAIRKSFKETFTQMFGGGSADLVLTETDLLSAGIEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFAIIRVKTIPFVILDEVEAALDEANVKRFGDFLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGITMQESGVSKIVSV"
BASE COUNT         4598 a         2456 c         3053 g         4454 t
ORIGIN      
        1 ttaagcaaac gattgcaccg tcttttttat ggtgttataa taaattgttt agtaacggtg
       61 ataagacgat aacagaaacg aggaatggta aatggtcatg atagaagtgt cccaccttca
      121 aaaaaatttt tccaaaacca ttaaagagcc aggattaaag ggagccttga agtcctttgt
      181 tcatccccaa agagaaatct ttgaggcggt taaggactta tcttttgagg tacccaaagg
      241 gcaaatttta ggttttattg gcgccaatgg tgcaggaaaa tcaacaacga ttaagatgtt
      301 aacaggtatt ttaaagccta catcaggtta ctgtcgcatt aacggaaaga ttccacaaga
      361 taatcgtcaa gattatgtga gagacattgg agctgttttt ggtcaacgaa cacaactatg
      421 gtgggatttg gccttgcaag agacctatgt cgttttaaaa gaaatttacg atgtgcctga
      481 aaaagccttt cgcaagcgca tggatttttt aaatgaggtt ttggacctga atgagtttat
      541 taaggatcct gtccggacct tatcactcgg tcagcggatg cgagctgata ttgctgcttc
      601 cttgttgcat aatcctaaag tattgttttt agatgaacca acgattggac ttgatgtttc
      661 tgtgaaggac aacattcggc gtgccattac tcaaattaat caagaagaag agacaactat
      721 tttactgacg acccatgatt tgagtgatat tgaacagctc tgtgatcgga ttatcatgat
      781 tgataagggc caagaaatct ttgatggcac ggtgacccaa ctcaaacaaa catttgggaa
      841 gatgaaaagc ttatcttttg aattggaagc gggacaggaa gaagttgttt ctcagtttct
      901 tggtttgcct gatataactg tggaacgaca cgaattaact ttggaaattc aatatgacag
      961 ttcgcgctat caaacagcgg acattattca aaaaaccata gctgattttg cggtcaggga
     1021 tttgaagatg actgatgtag atattgagga tattgttcgt cgtttctacc gaaaggagct
     1081 ttaagatgtg gtcattttgg aaacgttata gcccctttct cagtgcagga attcaggaat
     1141 taataaccta tcgcgttaat ttctttttgt atcgtattgg agatgtcatg ggggcttttg
     1201 tggcttatta cctctggaag gctgtttttg attcgtctaa gcaatcctta atcaatggtt
     1261 ttactctgtc tgacatgacc ttttacatta tcatgagttt tgtgaccact ttactgacca
     1321 aatcagacag ctcctttatg attggcgaag aagtgaaaga tggttctatc atcatgcgcc
     1381 tgcttcgacc agttcatttt gcagcctctt acctctttat ggaaattggt tttcgctgga
     1441 ttgtgctaat gagtgtcggc tttccttttt tagtggtctt atctggtatt aaagtcatgg
     1501 cggggctgtc tatcttgcaa gtcctagcca gcagttgtct gtatttagtt agccttttat
     1561 tagctttttt aatcaatttt tatttcaaca tttgttttgg cttttcagca tttgttttta
     1621 aaaacctttg ggggtctaat ttattgaaaa atgccttggt tgcctttatg tcaggcagtt
     1681 tgattccatt ggcttttttt cctaaaatgg tttctatagt gcttagtttt ctaccgttat
     1741 cctctttggt ttacacgcca gttatgattg tcattgggaa atacagcctt agtcagataa
     1801 tggttgccct aagtttgcaa atcttttggt tactggtgat ggtagtctta tcgcaagtca
     1861 tctggaaaaa ggttcagtac catttaacga ttcaaggagg gtgagatggc aaaattgaga
     1921 cgtatgcacg ctatttttat caaacaatac ctcaaacaaa ttatggaata caaggttgat
     1981 tttgtggtgg gagtgctagg tgtttttctc actcagggac ttaatctgct ctttttatcg
     2041 gtacttttcc aacatatccc ttctttagaa ggctggacgt ttgaacaaat tgcctttatt
     2101 tatggctttt ctttgattcc aaagggaatt gatcacctct tttttgacaa tctttgggct
     2161 ttagggcaac ggcttgtccg caaaggagaa tttgacaagt acctcaccag accaattagt
     2221 cctttgtttc atgttttggt tgagaccttt caagtggatg ccctgggtga attattggtt
     2281 ggggttatct tattagtgac cacggctggt agtatcgtat ggactcttcc taaggtgctt
     2341 ttgtttattt tggttattcc ttttgcgacc ttgatttaca cctctttaaa aattgcgacc
     2401 gccagcattt ctttttggac caagcagtct ggggcagtta tctatatttt ttacatgttc
     2461 aatgactttt ccaagtaccc tatgtccatt taccattcct ttttgcggtg gttaatcagt
     2521 tttatcattc cctttgcttt tacagcttat taccctgctt cttatttttt aacaggtcaa
     2581 cacctgctct ttaatattgg aggcttggta gtggtgtcgt tactggtttt ggccttgtcg
     2641 cttaagcttt ggaaatgggg cctagatgct tatgaaagtg caggttccta gagagactct
     2701 taaacaaatt gaaaatcgtt taggtcagaa tatgaaaacc tagataatga aggaaggcag
     2761 ctgttgtaaa aacggctgct tttttttgtg atggtttacc aagcgtaggc attggttgga
     2821 accatcatca gcagattggt ctaaattaag ggcggtaaaa taacataagc agatatattt
     2881 caagtggttg catgcaattg ctagataaca tttatggaac tttgagatct tttgccttta
     2941 tgagacctgt caaatctagt aacgatacca gcttagcaga tgccttgcag gctgatacag
     3001 acatggcttt tattttagtt agaggcatta actatcaata aatagtaaag acaaggtttg
     3061 tcaataccat aaaaaagtta agtgtcacag cttagctttt ttatggtgtc taattcttta
     3121 ttctatataa gattttcttt tcttaggaat ggttttccag tataataaag tcaccctgta
     3181 tcaaaaacaa aggttacagt ttgaatgtta aagcaacaaa gtaagtgact tctacgacaa
     3241 tagttgtggg acttgacatt tcaatgacgt tagagaattt tatagatttt attgatggca
     3301 cactagcgag actaaagaga ttctctaaat taatttaggt gtatttttag cagttattag
     3361 ttcaaaaaag aaaaccatca gaaataatta tataataacc atttaagcac atcgaaaata
     3421 aaggaggtca atataatgac ggatgtttat attgctgcag gccttagaac ccctatcggt
     3481 ttagtaggga aacaatttgc taaagaacaa ccagaaatcc ttggagcaaa gctcatcaac
     3541 gctttacaaa ataagtatcc agttcccatt gaccaagtga tttgtggcaa tgccgtcgga
     3601 acgggtggta atattgggcg cttgatgacc ctgtattctc atttaggaga atctgtctct
     3661 gctttgacgg ttgatatgca gtgtgcatca gctggtgcag ctctttccgt gggttatgct
     3721 aaaatcaagg caggtatggc aagcaacctt ttagtgggag ggattgaaag cagctcctta
     3781 caacctgaat cggtatatgc ttcagctgat tggcgccaag gagcctacaa ggtagctcag
     3841 ttttctccag atagtatcag cccttttgcg atgattgaag gggcagaacg agtggcaaga
     3901 gagcatggct ttacaaagga gtatttgaat cactggacat tgagaagtca tcaaaaggct
     3961 agttattgcc aggagcaagc cctgttagct gatcttattc tagacctgcc aggagccagt
     4021 gatcaaggta ttcgaccgcg cttgtcaagt aaggtgttgt caaaggtacc ccctatctta
     4081 ggagaagatc acgtgattag tgcagccaat gcctgcctaa cccatgatgc tgctgccttt
     4141 ttacagctaa gtagtcagcc ttcagctttt aaactgattg atgtggtaga agtagcagga
     4201 gacccacagc gtagcccttt aatggtgatt aaggctagtc aagtcctttt ggaaaaacat
     4261 ggtctaggga tggcagatat gacagcgatt gaatggaacg aagcctttgc cgttattgat
     4321 ggtttatttg aaacccatta cccagattta ttggaccgct ataatatatt tggtggtgca
     4381 ttagcttatg gtcatcccta tggtgcctca gcagccatta taatattgca cttaatgagg
     4441 gctttagaga ttaaaaatgg acactatgga atagctgcta tagcagcagc tggtggccaa
     4501 gggtttgctg ttttactaaa atatcataag gagtttagtc atgctgacaa agctagaata
     4561 ttgggcaaag caatgcccaa ataaaaaagc tatcgtagcc gatcagataa gcttgactta
     4621 tcaagagcta tggcaagcgg ttttaataaa ggatcagaca ataaaagaca gtgtaccata
     4681 tatcatcagt catagtcgct atcttaatca gttactctct tttttacgag gattaaaaga
     4741 aggcagttgt cctatcattt tacaccctaa tatctcaggt acatttcagc agcaaataaa
     4801 acacgtcgat ggtgagcttc ttaaaaaagc tgattttgct gttttgagtt cgggaacaac
     4861 tggcaaagca aaattatttt ggcgttgtct atcaacttgg acgagacttt ttgattatca
     4921 aaataaggtg tttggtatga ctggtaatag ttgtctattc ttacatggta gttttagttt
     4981 tactggcaat cttaacttag cattagctca actatgggcc gggggatgtc tggttttaag
     5041 ccaaaagctg tctttgaaaa cgtggttaag cttatggcaa gcaaaaaagg tgagtcacct
     5101 ttacctttta ccgacctacc taaatcgctt gctaccttat ttgactaaga acaacatgac
     5161 tgcgactcac ttattgacct cttcgcaaat gatatcacaa gaattactaa gacattatta
     5221 caaaaaattt cctcagcttg aaattgtcat tttctacggg gctagtgaat tatcttttat
     5281 aacgtggtgt aatggcagag ctgctgtcaa aattaatggt ttggtaggac agccctttcc
     5341 tgatgtgtct atcagtttca aagataagga aatttttgta gagactcctt atagtgtaga
     5401 aggaatgtca caaccataca gtgtctcaga cttaggaaaa atgagcccag cgggtttaat
     5461 tttagaaggg cgacaagatg actgggttaa tcaacgagga gtgaaatgcc acctgcctag
     5521 cctagttgaa ctcgctcacc aagcgccaaa cgtcaaagaa gcgcacgctt taaagatagg
     5581 aaaaggagaa aacgaaaccc taatattagt attagttttg actaagaagg attgcctagc
     5641 acctattaaa gactttttag ctctttattt aaactctgga caacttccca agtactatct
     5701 tgtgattgat tgtttgcctt taaaagataa tggtaaaatt aatcgagaag ttcttttaaa
     5761 taaaataccc aaacagtggc ttagttaacc acttcaggtt tcgttttgct tatagtgaga
     5821 tacaagtgac gattcagctt tagatgtgtt ataatatggt caaaaaagat gaggagaagg
     5881 ccatgaaaca agctatctta gatcgctatc aggccttgaa atgctatcaa aacgcaggtt
     5941 tatcaaacca agcatttcgt gctatagcta aagagcctat cattgataat cgacttggtt
     6001 ctccaacttt ctgggtaata tggcctattg aaaaagaaaa ccaatcagct aagcagttac
     6061 taacgtttct tcttgatttg gtagagatgc catttgagct aagtggtcag ttgcatgaaa
     6121 ctcaaacgct actcacccga ttccatccat ctctattgcc agatcatatg ttttggaaag
     6181 agttggctag cttagttgat caagcttttc ctggcaaaac actaagccaa gcaggtgagc
     6241 ttgaaaagcg tttgcatcaa tttcgttata tcatctctag tcaacaagct caatctatca
     6301 gaaaccatta taaaatgatt gagatgacag acgcccaagc tctggccctt tttttgaggt
     6361 ctaaaaaagg gccttgtttg tggcggcaag caccagatta taccttgatg gattctgcca
     6421 ggttacataa taaactaaga tttgaagata ataaagtcat ttttccaagt caagaagttt
     6481 cctataatat aaaagtactt ctttggtttc ataccgaatt tattctagat agcacaggct
     6541 tttttcttaa tgaagttgat gcagaagtag tgaccgaaaa aggaattgtc aatggtgcat
     6601 cttttaacta tggcacagat ggtcctagac attgggattt agatgttgat ccgattagcc
     6661 gtcacgatcc tcagtttaga agagacaccc ttaaaggatt tcgaacgcca aaaagagtat
     6721 ttcgtcagtg gtttagagct caaaaagatg actttatgtt ctcttatttt aatgccaaag
     6781 gactttttgc ttaccacaac aagagtagtt ttgccagagt caaaaaatct gctaagcaat
     6841 tcaaacgcca aattcatcct ataaagggtt ggttttagaa agtactctat ttgtaacggt
     6901 agtcgtcatt gtcaattagg ctgaattttt ggtataatca ggtagaaata atttagaaaa
     6961 atatagtttt gtgatttttt attaaccaca aaaatcacaa attgtaatat caattacaag
     7021 aactttctga ggtatagaca tgaaaaaaat acttattgtg gatgatgaaa aaccgatttc
     7081 tgacattatt aagtttaatt tgacaaaaga aggttatgac attgttacag cttttgatgg
     7141 acgcgaagcg gtaaccattt ttgaagaaga aaagccagat ttaattattc ttgatttgat
     7201 gctccctgag ttggacggtc ttgaagtagc caaggaaatt cgtaaaacca gtcatgtccc
     7261 gattattatg ttgtcggcta aagatagtga gtttgacaag gttattggac ttgaaattgg
     7321 ggctgatgat tacgtgacca agcccttttc taatcgggaa ttgctggcgc gtgtcaaggc
     7381 tcatctgcgt cgtaccgaaa ctattgaaac ggctgttgca gaagaaaatg cttcttcagg
     7441 tacacaggaa ctaaccattg gtaatttaca gattttacca gatgcgtttg ttgctaaaaa
     7501 acatggtcaa gaggtagagt tgactcatcg tgaatttgaa ctattgcatc atctagctaa
     7561 ccatatgggt caggtgatga cacgagaaca cttattggaa attgtttggg gatatgatta
     7621 ttttggcgat gtgcgcacgg ttgatgtgac tgttcgtcgt ctccgtgaaa aaattgaaga
     7681 cacaccaagt cgtcctgagt atattttaac aagacgtggt gttgggtact acatgaaatc
     7741 ttatgactag agatatcatt ggaaacttat ccacatttga attagcgatc cttatcttac
     7801 ttgtttttgt tgctttttac tttatccatc ttgcggtgcg tgattaccga aatgcacgta
     7861 ttattcggat gatgagccat aaaatccgag acttgattaa tggtcgctat actgatataa
     7921 tcgacgaaaa agcagacatt gagttaatgg agctttcaga ccagttaaat gacctgtcag
     7981 atgtttttcg cttgacgcat gaaaatcttg cccaagaaaa aaatcgcttg gcaagtattt
     8041 tggcttatat gtcagatggt gtacttgcta cagaccggtc tggtaaaatc atcatgatta
     8101 acgagacagc tcgcaagcaa ttaaatttaa gtaaagaaga ggcactaaag aaaaacatta
     8161 cagatttgtt agaaggtgat acttcatata cctaccgtga tttggtatcc aaaacaccag
     8221 tggtaactgt taatagccga aatgatatgg gtgagtttgt ttcattacgc ttgcgttttg
     8281 cgttgaatag gagagagagt ggttttattt cgggcttggt tgtggtgctc catgacacca
     8341 cagaacaaga aaaagaagaa cgtgaacgcc gtctttttgt ttctaatgta agtcatgaat
     8401 taaggacccc tttaacttcg gttaaatcct acttggaggc tcttgatgaa ggtgcactta
     8461 aagaagatat tgctccaagt ttcataaaag tttctcttga tgaaactaat cggatgatgc
     8521 gtatgatttc agatctttta aacctgtctc ggattgataa tcaagtaacc caattagcag
     8581 tagagatgac taattttact gcttttataa cttctatttt aaacagattt gatttggtta
     8641 aaaatcagca tacaggtaca ggaaaagtct atgaaattgt aagagattac cctattacct
     8701 ctgtctggct tgaaattgat aatgataaaa tgacacaggt tatcgagaat attttgaaca
     8761 atgccattaa gtattctcca gatggtggaa aaattacagt ccgtatgaaa acaacagata
     8821 cccaattaat tatttccatt tcagaccaag gactaggtat ccctaaaaca gatttgcctc
     8881 ttatttttga tcggttctat cgtgtagaca aggcaagaag tcgtgcccaa ggagggaccg
     8941 gtctaggcct tgccattgct aaagaaatca tcaagcagca ccatggcttt atctgggcta
     9001 agagtgacta tggtaaagga tcgaccttta ctattgtctt gccttatgaa aaagatgcag
     9061 ccatctatga agaatgggag gaagatgtag actaatcaat gaatgagagt ggttttaaat
     9121 atagtatttt agcatctggg tccacaggaa attgttttta tttagaaaca cctaaaaaac
     9181 gcttattgat tgatgcaggg cttactggta agaaaattac cagtcttctt gctgaaattg
     9241 accgcaaacc tgaggatttg gatgctattt tgattaccca tgagcactct gaccatatta
     9301 agggtgtagg cgttatggcc cgcaaatacc atttggatat ctatgctaat gaaaaaacat
     9361 ggcagctgat ggatgagtgc aatatgctag gtaagcttga tgcctcacaa aaacatattt
     9421 tccaaaggga taaagttttg acctttggag atgttgatat cgaaagtttt ggagtcagcc
     9481 atgacgctat tgatcctcaa ttttatcgga ttatgaaaga taataagtct tttgtgatgt
     9541 tgacagatac aggatatgtc agtgatagga tgacaggtat tattgaaaat gcagatggct
     9601 acttgattga atccaatcat gatattgaaa ttttgagatc aggctcttat ccttggagct
     9661 tgaagcagcg tattttatct gatttaggac atttatcaaa tgaagatgga gcgggagcca
     9721 tgattagaag cctggggtac aataccaaaa aaatatacct aggtcatctg agtaaagaaa
     9781 ataacatcaa agagttagcg catatgacga tggtcaatca actggctatg gcagatttag
     9841 cagtaggtac agactttacg gtccatgata cctctccaga tactgcttgt ccattaactg
     9901 atatttgaga taagaaacta aaagagaata tgcaaagtag cgctgattgg tgatatacca
     9961 gtcagggcta ttttttgatg tcttaaagcg tattgtcttc tgagttcaat gaactctcat
    10021 tggttatcct ttagagcatc aatagactta aattagcctg tagttttggc ttgtctgatc
    10081 tatttatcaa aggtgttgag gattataaag ataaggtagt tttttagttg ttcagactaa
    10141 aaacaaaata gctctatact atctgtagtg ggactgtagt gggactgtag tgggaaattc
    10201 ccctcagata gtgtagagtc ttttgattgt atgagaaagt ttcctttgac gggtatttaa
    10261 gatgtttaat aaatcacgaa aaatccactt agagtcggtt ttctaggtgg atttttggta
    10321 tactaaataa ggtctactga caaatatgaa agggattttt tatgaaacag cttgaagagt
    10381 tactctcaac atcttttgat attcaattta atgatttgac tttactagaa acagctttta
    10441 cccatacctc ttacgctaac gagcatcgtc tcctaaacgt ttcacataat gaacgcttgg
    10501 aatttttagg agacgctgtt ctacaattga ttatttcaga gtatttattt gcaaagtatc
    10561 ctaaaaagac agaaggagat atgtctaagt tacgttccat gattgtacgt gaggaaagct
    10621 tggcaggttt ttcacgattt tgttcttttg acgcttatat caaactagga aaaggtgagg
    10681 aaaagtcagg tggtcgccgt cgtgatacta ttttaggaga cttatttgaa gcctttttag
    10741 gtgctctttt attagataag gggattgatg cagtacgtcg cttcttgaaa caagtcatga
    10801 ttcctcaggt ggaaaaaggg aattttgaaa gggttaaaga ctataaaact tgtcttcaag
    10861 agtttttgca aacaaaaggt gatgtggcta ttgattatca agtcattagt gagaaaggac
    10921 ctgcccacgc taaacaattt gaggtatcga tagttgttaa tggtgctgtc cttagtaaag
    10981 gtttaggtaa gtctaaaaaa ttagcggagc aagatgctgc taaaaatgcc cttgcccaac
    11041 ttagtgaggt ttagatgttt ttaaaagaaa ttgagttgga agggttcaag tcctttgcgg
    11101 acaagactaa aattgaattt gataagggag taactgcagt cgtaggacca aatggttcag
    11161 gtaaaagtaa tattacagaa agtttacgtt gggctttagg agaatcaagt gctaaaaatc
    11221 tgcgtggggg caagatgcca gatgtcattt ttgctgggac acaaaaccgt aatcctctaa
    11281 actatgctaa ggtagcggtt gttcttgata attccgatca ttttataaaa actgccaaga
    11341 aagaaatccg tgtcgaaagg catatttacc gaaatggaga tagtgactat ctgattgatg
    11401 gtcgcaaggt aaggctccgt gatattcatg atttgtttat ggatacaggg cttggtcgtg
    11461 attctttctc cattatttct caaggtcgtg tagaggaaat tttcaatagt aaacctgaag
    11521 aaaggcgcgc catttttgaa gaagcagctg gtgttttaaa atataaaaca cgtaaaaaag
    11581 aaactcagat taaattaaat caaactcaag ataatttaga ccgtctagag gatattattt
    11641 atgagctaga cacgcagttg gccccacttg aaaagcaagc taaagtagct aaacaatttt
    11701 tagaacttga tgccaatcgt aagcaactac aactagatat tctagtcaaa gatattgata
    11761 tagctcaaga aagacagact aaagatactg aagctttagc tgctttacaa caagacttag
    11821 catcttatta cgctaagcgt caatctatgg aagaagatta ccaaaaattc aagcaaaaaa
    11881 agcaggttct tagtcaagag tcagatcaaa cccaaacaac attattagaa ttaaccaagc
    11941 taatagctga cttggaaaag caaattgaat tggttaagct agaaagtggc caagaagcag
    12001 agaaaaaggc agaagccaaa aaacatctcg aacagttaca agaacaattg gatggttttc
    12061 aagcagaaga aaagcagcgt acagaacaat tattgcatat tgatcaacaa ctttgtgatg
    12121 tcaaacaaca gttaaacgaa ttgagcaatg cattagagcg tttttcaagt gatcccgacc
    12181 aattaatgga aactctaaga gaagagtttg tattattgat gcaaaaagag gcggccttat
    12241 caaatcagct gacagcttta aaagcccatt tagacaaaga aaaacaggct cgtcaacata
    12301 aggctcaaga atatcagcta cttgttacta agcttgatca acttaatgac gagtctcaaa
    12361 aagctcaagc tcattataaa gctcaaaaag agcaagtcga aatgttgctc caaaattatc
    12421 aagaaggtga taaacgcgtt caggaattag aaagagatta ccaactgaat caagaaagac
    12481 tatttgactt gttggatcaa aaaaaaggaa aagaagctcg taaggctagc ttagaatcta
    12541 ttcaaaaaag tcatagtcaa ttctatgcgg gagtacgagc tgtccttcaa tcacaaaaaa
    12601 agcttggagg gatcataggt gcagtcagtg aacacctttc ttttgattct gattaccaaa
    12661 cagctcttga aattgcatta ggtgctaata gtcagcatat tattgtaaca gacgaagctg
    12721 cagcaaaaag agcaattgct tatttgaaaa aaaatcgcca aggacgagca acttttttac
    12781 ctttaactac tataaaggct agatcattgt cagaacacta tcatcgtcaa ttagcgactt
    12841 gtgaaggtta tctgggaact gcagaatcat tggttcgcta tgatgatagt ctttcagcta
    12901 ttatccaaaa ccttttatct agtactgcta tctttgaaac tattgaccaa gctaatattg
    12961 cagcgcgctt attaggatat aaggtaagaa ttgttactct tgatggaaca gaattacgac
    13021 caggtggttc tttttcagga ggagctaata gacaaagcaa tacaactttt atcaaaccag
    13081 agttagaaca aatcagtgag gaattaacgc gattagttga gcaattaaaa ataactgaaa
    13141 aagaagtagc tgccttgcaa agcgatttga tagcaaaaaa agaagaactg acacaattga
    13201 aattagccgg tgaccaggca cgcttagctg aacaaagagc tcaaatggct tatcagcaat
    13261 tgcaagaaaa acaagaagat tcaaaggctt tattagctgc ccttgatcaa agtcagacga
    13321 ctcattctga tgaatcttta ttggcagagc aagctcgtat tgaagaagca ttaacagcta
    13381 ttgctaagaa aaaaagtgcc ttaacttgtg atattgatga tatcaaagaa aacaaagatt
    13441 tggttaggca aaaaacacaa aacattcacc aagctttatc tcaagctcgt ttgcaagaaa
    13501 gagatttact taatgaaaag aaatttgagc aagctaatca atctcgtcta agaactcagc
    13561 tgaagcaatg ccagcaaaat attctcaaac ttgaaagcat actaaacaac aacgtaagtc
    13621 aagacagcat tcaaagacta ccccagtggc aaaaacagtt acaagatgcc acagagcata
    13681 agtctggtgc tcaaaaacgt ttagtccaat taagatttga aatagaggac tatgaggccc
    13741 gcttagaaga aacagcagaa aaaataacca aagaaagcga aaaaaatgac acatttattc
    13801 gtcgtcaaac caagttagag acacatttag agcaagtggc taatcgctta cgagcctatg
    13861 ctaaaagttt atcagaagat tttcaaatga cactagcaga cgcaaaggag gtaacaaata
    13921 gtattgatca cttagaaagt gccaaagaaa aattacacca tttacaaaag actattcgtg
    13981 cactaggacc aattaatagt gatgctatta ctcagtatga ggaagtgcat gagcgtttga
    14041 cttttttaac aagtcaaaag acagatctga caaaagctaa aaatcttttg ctagagacta
    14101 ttaatagtat ggatagcgaa gtgaaggctc gtttcaaaat gacttttgag gcaattcgaa
    14161 aaagttttaa ggaaaccttt actcagatgt ttggaggggg ttctgctgat ttggttttaa
    14221 cggaaacaga cctcttgtct gctggtatag agatttctgt gcagcctcct ggtaagaaaa
    14281 tccaatctct taacttgatg tcaggcggag aaaaagcttt gtcagcactg gctttattgt
    14341 ttgctattat acgtgttaaa acgattccgt ttgtgatttt agatgaggta gaagcggctc
    14401 ttgatgaagc aaatgttaag cgctttgggg atttcctcaa tcgctttgac aaagatagtc
    14461 aatttattgt cgtgacccat cgtaaaggaa caatggctgc agcagatagt atttatggaa
    14521 ttaccatgca ggaatcgggg gtctctaaaa ttgtatctgt a
//
LOCUS       DAUCLR010000043        14525 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.043, whole genome shotgun sequence.
ACCESSION   DAUCLR010000043 DAUCLR010000000
VERSION     DAUCLR010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14525
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.043"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(1..444)
                     /locus_tag="VGT87_001098"
                     /pseudo
     CDS             complement(1..444)
                     /locus_tag="VGT87_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370026.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
     gene            complement(961..1593)
                     /locus_tag="VGT87_001099"
     CDS             complement(961..1593)
                     /locus_tag="VGT87_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEQ3813960.1"
                     /translation="MVENTKLLCSWVWLLALAILITIYSTWLWYPLEVDHLKLEQVVF
                     MSKDAILHNYNGLLNYLTNPFVTRLEFANFHSSADGLKHFADVKWLFHLTQVVFLGLL
                     YPTLKTFTQRLKTKRFWLLQKPLILAALFPLMIGLMASFIGFEHFFTLFHQVLFVGDS
                     SWLFDPLKDPVIWILPEVFFLHCFLFFMIVYEIILWSLVGLARWQRLNVE"
     gene            complement(1593..2357)
                     /locus_tag="VGT87_001100"
     CDS             complement(1593..2357)
                     /locus_tag="VGT87_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEQ3813961.1"
                     /translation="MPYKGYLIDLDGTIYQGKNRIPAGERFIKRLQEKGIPYLLVTNN
                     TTRTPEMVQSMLTNQFHVETGIETIYTATMATVDYMNDMNRGKTAYVIGETGLKSAIA
                     AAGYVEELENPAYVVVGLDSQVTYEMLAIATLAIQKGALFIGTNPDLNIPTERGLMPG
                     AGALNALLEAATRVKPVFIGKPNAIIMNKSLEVLGIQRSEAVMVGDNYLTDIMAGIQN
                     DIATILVTTGFTRPEEVPTLPIQPDHVLSSLDEWRL"
     gene            complement(2357..3109)
                     /locus_tag="VGT87_001101"
     CDS             complement(2357..3109)
                     /locus_tag="VGT87_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEQ3813962.1"
                     /translation="MGLSYQEELTLPFELCDVKSDIKLPLLLDYCLMVSGRQSAQLGR
                     SNNNLLVDYKLVWIVTDYEITIHRLPHFQETITIETKALSYNKFFCYRQFYIYDQEGG
                     LLVDILAYFALLNPDTRKVATIPEDLVAPFKTDFVKKLHRVPKMPLLEQSIDRDYYVR
                     YFDIDMNGHVNNSKYLDWMYDVLGCAFLKTHQPLKMTLKYVKEVSPGGQITSSYHLDQ
                     LTSYHQIISDGQLNAQAMIEWRAIKQTESEID"
     gene            complement(3119..4249)
                     /locus_tag="VGT87_001102"
     CDS             complement(3119..4249)
                     /locus_tag="VGT87_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEQ3813963.1"
                     /translation="MSKKPTSAYVHIPFCTQICYYCDFSKVFIQNQPVDAYLKALIQE
                     FESYDIHDLKTLYIGGGTPTAITAKQLEYLLNHLERNLNLDDLEEFTIEANPGDLTPE
                     KIAVLQRSAVNRISLGVQTFNNKQLKQIGRSHNEEQIYSTIANLKTAGFHNISIDLIY
                     ALPGQTLEQVKENVAKALALDIPHLSLYSLILEHHTVFMNKMRRGKLNLPTEDLEAEM
                     FEYIISEMEANGFEHYEISNFTKPGFESRHNLMYWDNVEYFGCGAGASGYLNGIRYQN
                     RVPIQHYLKAVEAGNARLNEEVLRKEEMMEEELFLGLRKKTGVSIQRFQEKFGISFEE
                     RYGNIVRELQNQGLLVKDDAFVRMTKKGLFLGDSVAERFILD"
     gene            complement(4312..4953)
                     /locus_tag="VGT87_001103"
     CDS             complement(4312..4953)
                     /locus_tag="VGT87_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813964.1"
                     /translation="MTKQQIEKLADLLPPFAATLYLDMIPEFQEGYASYVYQTPKQDI
                     QKRRIKQLTKNFERLLDTPSFYVMPVTRKTAEQLLADKGLAYHLLSTVAANQVFAAAV
                     NEDQTLFQVLRNGARVAFVILQEKGEFLEMTLGILETVTENEVPAAIVEAVQTYLLTD
                     MQEKLPSLSIMVSQESSKLFYQEQGFVLLAKGCKPGNENDSDVCQLVKYFSKP"
     gene            complement(5077..6432)
                     /locus_tag="VGT87_001104"
     CDS             complement(5077..6432)
                     /locus_tag="VGT87_001104"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEQ3813965.1"
                     /translation="MGKYFGTDGVRGEANVELTPELAFKLGRFGGYVLSQHETERPKV
                     FVARDTRISGEMLESALIAGLLSVGIEVYKLGVLATPGVSYLVRTEKASAGVMISASH
                     NPALDNGIKFFGNDGFKLADDQELEIEALLDAPEDTLPRPSAEGLGTLVDYPEGLRKY
                     EKFLVTTGTDLSGMTVALDTANGAASVSARDVFLDLNAEIAVIGEKPNGLNINDGVGS
                     THPEQLQELVKETGADLGLAFDGDSDRLIAVDETGEIVDGDRIMFIIGKYLSEKGLLA
                     HNTIVTTVMSNLGFHKALDKQGINKAITAVGDRYVVEEMRSSGYNLGGEQSGHVIIMD
                     YNTTGDGQLTAIQLAKVMKETGKSLSELAAEVTIYPQKLVNIRVENSMKDRAMEVPAI
                     ANIIAKMEDEMAGNGRILVRPSGTEPLLRVMAEAPTDAEVDYYVDTIADVVRTEIGCD
                     N"
     gene            complement(6486..7441)
                     /locus_tag="VGT87_001105"
                     /pseudo
     CDS             complement(6486..7441)
                     /locus_tag="VGT87_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984857.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
     gene            complement(7438..8289)
                     /locus_tag="VGT87_001106"
     CDS             complement(7438..8289)
                     /locus_tag="VGT87_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEQ3813966.1"
                     /translation="MNNLSSIDIKFLLSLFADPWLLAVHLLDILIVAYLIYRFIKALT
                     GTKIMSLVQGVIFFLVLRFIAEWIGFTTITYLMNQVITYGVIAGVVIFTPEIRAGLEK
                     FGRSTQVFLQKQYVSSESALVDALIKSVAYMGPRKIGALIAIEQTQTLQEYIATGIPL
                     NADISSQLLINIFIPNTPLHDGAVIVGQNKIVAACAYLPLSESKAISKEFGTRHRAAI
                     GLSENSDALTIIVSEETGAISVTRKGQFLHDLSTDEFETVLRTYLMSDSNVSLPWYKK
                     ILGGKSK"
     gene            8396..9739
                     /locus_tag="VGT87_001107"
     CDS             8396..9739
                     /locus_tag="VGT87_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEQ3813967.1"
                     /translation="MKMKTLLGIIAGKAAQSILTKLGRGSTYPGRLALACNKDILKDL
                     SKDYDIVVVTGTNGKTLTTALTVGILKEAFGEIITNPSGANMITGITSTFLAAKKGKS
                     GRQIAVLEIDEASLPRITTYLKPSLFVYTNIFRDQMDRYGEIYTTYQMIVDGARNAPK
                     ATILANGDSPIFSSKDIVNPVQYYGFDTAKHAPQLAHYNTEGILCPKCEHILQYRLNT
                     YANLGDFICLNCGFKRPQLDYRLTELTTITNHSSEFIIDGQDYKINVGGLYNIYNALA
                     AVSVAEFFGVSPEKIKAGFNKSKAVFGRQETFTIGDKSCTLILIKNPVGASQALEMIQ
                     LADYPFSLSVLLNANYADGIDTSWIWDANFELITQMPITEINAGGVRHSEIARRLRVT
                     GFDDTKIKQAEKLEQIIETIEKQEAKHAYILATYTAMLEFRSLLADRHVVEKEMI"
     gene            9739..10530
                     /locus_tag="VGT87_001108"
     CDS             9739..10530
                     /locus_tag="VGT87_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEQ3813968.1"
                     /translation="MTYTSLKSPENQDYIYDLTIAHLYGNLMNTYGDNGNILMLKYVA
                     EKLGARVTVDIVSINDTFEQDDYDIVFFGGGQDYEQSIVAKDLPSKKAALADYIANNK
                     VVLAICGGFQLLGQYYVQANGVKIDGLGIMGHYTLNQHQNRFIGDIKIHNDEFNETYY
                     GFENHQGRTFLSGDEKPLGRVVYGNGNNKEDQTEGVHYKNVYGSYFHGPILSRNVNLA
                     YRLVTTALKKKYGSAISLSSYDDILKQEITEEYADLKSKASFNKV"
     gene            complement(10639..11628)
                     /locus_tag="VGT87_001109"
     CDS             complement(10639..11628)
                     /locus_tag="VGT87_001109"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007893175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEQ3813969.1"
                     /translation="MKYIVNKSHNPAFNIALEAYAFRELVEEDELFILWINEPAIIIG
                     KHQNTIQEINKEYIDEHGIHVVRRLSGGGAVYHDLNNLNYTIISNKTAEGAFDFKTFS
                     QPVIATLADLGVTANFTGRNDIEIDGKKICGNAQAYYKGRMMHHGCLLFDVDMTVLGD
                     ALKVSKDKIESKGVKSVRARVTNILNELPEKITVEEFSDKILTKMKETYPDMTEYVLS
                     EDELAKIEQSAKEQFGSWDWTYGKAPEYTIGRNVRYPAGKISTFANVENSIIKNLKIY
                     GDFFGIKDVQDIENLLIGCKYEYRDVFERLKTIDTTQYFSRMTVEEVAKAIVS"
     gene            11861..14278
                     /gene="hylA"
                     /locus_tag="VGT87_001110"
     CDS             11861..14278
                     /gene="hylA"
                     /locus_tag="VGT87_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronidase"
                     /protein_id="HEQ3813970.1"
                     /translation="MNTYFCTHHKQLLLYSNLFLSFAMIGQGTAIYADTLTSNSEPNN
                     TYFQTQTLTTTDSEKKVVQPQQKDYYTELLDQWNSIIAGNDAYDKTNPDMVTFHNKAE
                     KDAQNIIKSYQGPDHENRTYLWEHAKDYSASTNITKTYRNIEKIAKQITNPESCYYQD
                     SKAIAIVKDGMAFMYEHAYNLDRENHQTTGKENKENWWVYEIGTPRAINNTLSLMYPY
                     FTQEEILKYTAPIEKFVPDPTRFRVRAANFSPFEANSGNLIDMGRVKLISGILRKDDL
                     EISDTIKAIEKVFTLVDEGNGFYQDGSLIDHVVTNAQSPLYKKGIAYTGAYGNVLIDG
                     LSQLIPIIQKTKSPIEADKMATIYHWINHSFFPIIVRGEMMDMTRGRSISRFNAQSHV
                     AGIEALRAILRIADMSEEPHRLALKTRIKTLVTQGNAFYNVYDNLKTYHDIKLMKELL
                     NDTFVPVQKLDSYVASFNSMDKLALYNNKHDFAFGLSMFSNRTQNYEAMNNENLHGWF
                     TSDGMFYLYNNDLGHYSENYWATVNPYRLPGTTETEQKPLEGTPENIKTNYQQVGMTS
                     LSDDAFVASKKLNNTSALAAMTFTNWNKSLTLNKGWFILGNKIIFVGSNIKNQSSHKA
                     YTTIEQRKENQKHPYHAYVNNQPVDLNNQLVDFTNTKSIFLESDDPAQNIGYYFFKPT
                     TLSISKALQTGQWQNIKADDKSPEAIKEVSNTFITIMQNHTQDGDRYAYMMLPNMTRQ
                     EFETYISKLDIDLLENNDKLAAVYDHDSQQMHVIHYGKKATMFSNHNLSHQGFYSFPH
                     PVKQNQQ"
BASE COUNT         4617 a         2981 c         2505 g         4422 t
ORIGIN      
        1 cggattatct tgcaagagaa tagcggagct accgagatta ctttgatgag cccaaaggac
       61 aatctcacgc ctagcagagt ctcttgaatg agacccatac gccatgttaa aaatacctcc
      121 atcatcacca agagcctgag caaaattccc acgaatggtt ccgcaaagag catccgtagg
      181 attggttact ctcatcatgc atggttcaaa aagacaaaac caccttagtc cccaagaatt
      241 atcccaatta aaacaggacc acttgtcatg taagctttaa gtttaggatc aaaaggttta
      301 tcaccaaggt ctcgtcgtgc ttagccaagt atttcgaact agcttgtctt agctcgaatc
      361 gctcaaacgt aaatcttagg cgttcaatcc accgcaaaat ctctccaact aaccctctcg
      421 cttaacccca tctggcttaa tcataaaaaa atgttggttc catcactcta ctcttctttt
      481 gtctattata acatgaaaac gctttaacaa aaagaatatg aaggtatccg actgctggta
      541 tgaaccctct ttctcaagtt atcattggca atgttgaatg gagaccactc aaaacagcat
      601 agctctaaaa cccagagatg gcaaagattc ggtttactct agttttggga ccactcaaaa
      661 cagcatagct ctaaaaccca tctaaaatta tttgtgggct gtctgacgtt ttgggaccat
      721 tcaaaacaac atagctctaa aacgttcctg taattttgat ttgttccata ttgttttggg
      781 accattcaaa acagcatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
      841 ggcaccgagt cggtgctttt tttgatactt ctattctact ctgactgcaa actaaaaaac
      901 aagcgctttc aaaacgcttg ttttatcatt tttagggaaa ttaatctctt aatcctttta
      961 tcattctaca tttaggcgct gccatcttgc taaacctact aagctccaca ggatgatttc
     1021 gtaaactatc ataaagaaaa ggaaacagtg caagaaaaag acttctggaa ggatccaaat
     1081 cacaggatct ttcaagggat caaagagcca gctgctatct cctacaaaaa gcacctgatg
     1141 aaaaagagta aaaaaatgtt caaacccaat gaaactagcc attagaccaa tcataagagg
     1201 aaacaaagct gctagtatta aaggtttttg aaggagccag aaacgtttgg tttttaacct
     1261 ttgtgtgaaa gtcttaaggg ttgggtaaag gaggccaaga aaaacgactt gagttaggtg
     1321 aaaaagccac ttcacatctg caaaatgctt gagtccgtct gctgatgagt gaaaattagc
     1381 aaattccaac cttgtcacaa aaggattggt gaggtaattt agtagtccgt tatagttatg
     1441 caagatagca tccttgctca taaaaacaac ttgttctaac tttagatggt ccacctcaag
     1501 aggataccac agccacgttg aatagatcgt tatgagaata gctaacgcta ataaccatac
     1561 ccagctacat agtaatttag tgttttctac catcacaacc tccactcatc aaggctagat
     1621 aggacatgat ctggctgaat tggtaaggtt ggtacctctt caggtctggt aaagcctgtt
     1681 gtcacgagga tcgttgcaat atcattttga atacctgcca tgatatcagt caagtaatta
     1741 tcgccaacca tgacggcttc agagcgctga atgcccaata cctctagtga tttattcata
     1801 atgatagcgt tgggcttgcc aataaaaaca ggttttacac gtgtggcagc ttctagtaag
     1861 gcattcaatg cacctgctcc tggcatcaaa ccacgttcgg ttggaatgtt taaatcagga
     1921 tttgtcccaa taaagagggc acctttttga atagctaagg tggctatagc caacatctca
     1981 taggtcacct gactgtctaa accaaccacc acgtaagcgg gattctcaag ttcttctacg
     2041 taacctgctg ctgcaatagc tgatttgagc cctgtttctc caataacgta agctgtcttg
     2101 ccacggttca tgtcattcat gtaatcgacg gttgccatgg tagccgtata aatggtctca
     2161 atgcctgtct caacatgaaa ctgattagtc agcatgcttt gcaccatttc gggtgttctt
     2221 gtggtattat tagtcactaa taaataggga atgccttttt cttgtaggcg tttgataaac
     2281 cgctcccctg ctggaatacg gtttttccct tggtaaatcg ttccgtctaa atcaattaaa
     2341 taacccttat aaggcactag tctatctcgc tttctgtttg tttaatcgct cgccattcaa
     2401 tcatggcttg ggcattcagc tgcccatctg agatgatttg atggtaagaa gttaattggt
     2461 ccaaatggta actggaagta atttgaccgc ctggtgagac ttctttaaca tatttcaaag
     2521 tcatcttaag aggctgatgc gtttttaaaa acgcacaccc caacacatca tacatccaat
     2581 ctaaatattt actgttgttg acatgaccat tcatatcaat atcaaaataa cgcacataat
     2641 aatcacgatc aattgattgt tctaaaagag gcattttagg aacacggtgt agctttttaa
     2701 caaaatcagt cttaaaaggc gctactaaat cttctggaat agttgccact tttcgcgtat
     2761 ctgggtttaa caaagcaaaa taggctaaga tatccactaa aagacccccc tcttgatcat
     2821 aaatataaaa ttggcgataa caaaaaaatt tattatagga aagggctttt gtttcaatgg
     2881 tgatggtttc ttgaaaatgt ggcaagcgat gaatagtgat ctcataatcc gttacaatcc
     2941 aaacaagctt gtaatcaact aaaagattgt tgttacttcg tcctaattgc gcagactgtc
     3001 taccagaaac catcaaacaa tagtctaata aaaggggcaa ttttatatct gatttgacat
     3061 cacataattc aaaaggaagt gtcaactcct cctgataact taatcccata atactctcct
     3121 aatctaatat aaaccgctca gcaacagaat cacctaagaa aagacccttt ttagtcattc
     3181 tgacaaaggc gtcatctttg acaagcagtc cttggttttg aagctcccta acaatatttc
     3241 cataacgctc ctcaaaagaa ataccaaatt tttcttggaa ccgctggatg gagactcctg
     3301 tctttttacg taagcccaaa aagagttcct cttccatcat ttcctctttg cgcagcactt
     3361 cttcatttag tctagcgttt cctgcttcaa ccgctttaag ataatgctgg atgggcaccc
     3421 gattttgata acgaatgcca ttaaggtaac ctgaagcccc tgcaccacat ccaaaatact
     3481 ccacattatc ccaatacatc aagttatggc gactttcaaa acctggtttg gtaaaattgg
     3541 aaatttcata atgctcaaaa ccattagctt ccatttctga aataatatac tcaaacattt
     3601 ctgcttccaa gtcttccgtt ggtaagttaa gtttaccacg ccgcattttg ttcataaaaa
     3661 cggtgtgatg ttccaaaata aggctataaa gactaagatg gggaatatca agtgccaaag
     3721 ctttagcaac attttccttg acttgttcta aggtttgacc tggcaaggca taaattaaat
     3781 caatggaaat attgtgaaag cctgcagttt ttaaattggc aatggtgcta taaatttgtt
     3841 cttcattatg gctacggcca atctgcttta attgcttgtt attaaaagtc tgcaccccca
     3901 aggaaatccg attgacagct gacctctgaa gcaccgcaat tttttctggc gtcaaatcac
     3961 caggatttgc ctcaattgtg aattcttcaa gatcatctaa attgaggttt cgttcaaggt
     4021 gatttaggag atactccaac tgtttagcag tgatagctgt aggcgtacct cctccaatat
     4081 aaagagtctt taagtcatga atgtcatagg attcgaattc ttgaatcaag gctttcaaat
     4141 aagcatcaac aggttgattt tggataaaga cttttgaaaa atcgcaataa taacaaattt
     4201 gtgtgcaaaa aggaatgtgc acataggctg aagtaggttt ttttgacatg gtcttattat
     4261 accactttga tgataaaatg tttctatgaa taaaccattt cccttgattc ttcatggctt
     4321 ggaaaagtat tttacgagct gacaaacgtc agagtcattt tcgtttcctg gcttgcaacc
     4381 cttagctagc aaaacaaacc cttgttcttg gtaaaaaagc ttgcttgatt cttgactgac
     4441 cataatgctt aaagaaggga gtttctcttg catatctgtc agaagatatg tctggacagc
     4501 ctccacaata gctgctggaa cctcgttttc tgtcactgtt tcaagaatcc ctaaagtcat
     4561 ttctagaaat tcaccctttt cttgaagaat cacaaaggct actcttgctc catttcgaag
     4621 cacttgaaaa agcgtctgat cctcgttaac agcagccgca aacacttgat tagctgcaac
     4681 tgtcgataac aaatgataag ctaggccttt atctgctagc aattgctctg ctgtttttct
     4741 tgtcacaggc atcacataaa aagatggggt atctagtaat cgttcaaaat ttttagttaa
     4801 ctgcttgatg cggcgctttt gaatatcttg ttttggtgtt tgataaacat atgaagcata
     4861 accttcctga aattcaggaa tcatatctaa gtaaagtgtg gctgcaaaag gaggcagtaa
     4921 gtccgctagt ttttcgattt gctgttttgt catccgatat cctttctctt ctactttagc
     4981 aatttataaa aagagacttg ggctaaacgc cccaagcctc tttccaattg ttgacagaag
     5041 ccttagaaat aaggtttctt gtttgtactc aactcgttag ttatcgcatc cgatttctgt
     5101 acggacgaca tcagcaatag tatcgacata ataatcaact tcggcatctg ttggcgcctc
     5161 tgccatgaca cgaagaagag gttctgtacc acttggtctc acgagaatac gaccattacc
     5221 agccatttca tcctccattt ttgcaatgat gttcgcaata gctggaactt ccatagcacg
     5281 gtctttcatg ctgttttcca cacgaatatt caccagtttt tgagggtaaa tggttacttc
     5341 agcagctaat tccgaaagtg atttcccggt ttccttcatc accttggcta attggatagc
     5401 tgtcaactga ccatctccag tggtattgta gtccatgata atgacatgac ctgattgttc
     5461 accaccgaga ttgtatcctg agctacgcat ttcttcaacc acatagcggt ctccaactgc
     5521 tgtaattgct ttattaatac cttgtttatc taaagcttta tgaaaaccga ggttagacat
     5581 caccgttgtg acaatggtat tgtgagcaag aagccctttt tctgacaaat atttaccaat
     5641 gataaacata atacgatcac cgtcaacaat ctcaccagtt tcgtccacgg caatcagacg
     5701 gtcgctatcg ccatcaaagg ctaaaccaag atctgctcct gtttccttaa ctaactcttg
     5761 aagttgttct ggatgcgttg aaccaacacc gtcattaata ttaagaccat ttggtttttc
     5821 gccaatgaca gcaatttctg catttaaatc taaaaagaca tcacgtgctg acactgaagc
     5881 agctccatta gcagtgtcta aggcaactgt cataccgctt aagtcagtac ctgttgttac
     5941 caagaacttt tcatatttac gaagtccttc tggataatct accaaggttc caagtccttc
     6001 tgcactcggg cgaggaagcg tgtcctccgg agcatctaag agagcttcaa tttccaattc
     6061 ttggtcatca gctaacttaa acccatcatt cccaaagaat ttgatgccat tgtcaagcgc
     6121 agggttatga cttgcagaaa tcatcacacc tgcacttgct ttttctgttc taactaaata
     6181 agagacacca ggtgtcgcta aaacaccaag tttataaact tcaataccaa cagatagtag
     6241 accagcaatc aaggctgact ccaacatttc tcctgaaata cgggtgtcac gcgccacaaa
     6301 aactttaggt ctttcggtct catgttgact taaaacataa ccaccgaatc ggcccagttt
     6361 aaaagctaac tcaggtgtca gctcaacatt tgcttcacca cgaacaccat ctgttccaaa
     6421 atattttccc ataatttata taagcctaat ggcttcctcc attcaatttt cctagttatt
     6481 atccattatt ttttggtggt tgtcaactga acggtcacta ctgacggctc aattgacacc
     6541 tgactagaag acaaactcac ggtcttacta gtatttttag tgacatcaga aatgttaact
     6601 tctgcaataa tttcatcaat atcttctaag acctctcgac tacctgagat aacagctgtt
     6661 tgttttgaca atttgtattg aatatctgat aagctatcat ccatcatccc cactgcctca
     6721 actctaatcg ggactgattt cgttattttt ttacagcaac tgataaattt gtttttgcag
     6781 gatcaattgc acttgctaaa atagttccat cagctgagac agcttgtaag gtcactcgac
     6841 tactataatt cctatctaac acttgatcat caggtaactt tgccacaacg tgatcaatta
     6901 aagcaatggt agattcatca ctggtcactt caaccttatt aacccccgtt tcaattttgc
     6961 taatttcata accatttgca atttgtttag catcaacgct tcctctaaca ggaaatacct
     7021 tagattcttt tttgccaatt gtgactgata ttttatctgg agatacggta gctgtcaccc
     7081 cagaaggaag gttttctact cttaggttga cacttactgt acctggataa ctatgtgtta
     7141 gatccgccac aatcttaaaa ttacgtgtat cattgttcac ttcagaatct aatttgatac
     7201 gattagtgga ggttagataa acttccgcac cataagaata cccactgata aaatatttgt
     7261 cactatcata cttcatatca attggtacat ctttaaggct atgcgtatag gtttcaatcg
     7321 ggctataaat ttgcgagcta gaattattat gattgctcga tgctgcagtt aaaaaaagga
     7381 gaatcgcaaa aaagactgat accattccta accaaggacg actatttaaa aatcgtttca
     7441 tttagatttc cctcctagaa ttttcttata ccatggtaaa ctaacatttg aatcactcat
     7501 caggtaggtc ctaagtaccg tttcaaattc atctgtgctt aaatcgtgta aaaattggcc
     7561 tttgcgagtt actgaaatag ctcctgtttc ttctgaaaca atgatagtta gtgcatcaga
     7621 gttttcagat aatccaatag ccgctctatg acgggtccca aattctttgg aaattgcctt
     7681 tgattctgac aaaggcaagt aagcacaggc tgccacaatc ttattctgac caacgataac
     7741 agctccatca tgaagcggcg tattcggaat aaaaatattg atgagtagtt gacttgaaat
     7801 gtcggcattc agcggaattc ctgtcgcaat atattcttgt aaggtttgag tctgttcaat
     7861 agcaatcaat gcacctatct tgcgtggacc catataggcc acagatttaa tcaaagcatc
     7921 gaccaaagca gattcactgc tgacgtactg tttttgtaaa aaaacttgcg tagaacgacc
     7981 aaatttctcc agtcctgctc gaatttcagg ggtaaaaatg actactcctg caattacacc
     8041 ataagtaatg acttggttca ttaagtatgt gattgtcgta aagccaatcc actctgcaat
     8101 aaaacgaaga accaaaaaga aaatgacccc ttgaactaaa gacatgattt tagttccagt
     8161 caatgcttta ataaaacgat aaatcaaata ggcaacaatt agaatatcta aaagatgcac
     8221 tgccagcagc caaggatccg caaataagct taataaaaat ttaatatcga tactagataa
     8281 attattcata caactctcat ttctatatca aaaaagttat tagctatatt atatcatatc
     8341 atgatcaaag atttttccct atctaaaatc aataaactta tgataaaata taagcatgaa
     8401 aatgaagaca ttactcggca ttattgctgg caaagctgct caatccattt taacaaaact
     8461 agggcgagga tctacttatc ctggtcgatt ggctctagct tgtaataaag atattttaaa
     8521 ggatttgtct aaagactatg atattgtggt cgtcactggt actaatggta aaacgttaac
     8581 aactgctctt actgtcggta ttttgaaaga ggcatttggc gagataatca caaatccaag
     8641 tggtgccaat atgattactg gaattacttc tactttttta gctgctaaaa aagggaaatc
     8701 tggaaggcaa attgctgttt tagaaattga cgaagctagt cttcctagaa ttaccactta
     8761 cttaaaacct agtctttttg tttatactaa tatttttagg gatcagatgg accgctacgg
     8821 agagatttat actacttatc agatgattgt agatggtgct cgaaatgctc caaaagcaac
     8881 tattctagcc aatggagata gtcctatttt ctcttctaag gacatagtta atccagttca
     8941 gtattatggt tttgatactg ctaagcatgc tccgcagcta gctcattaca acacagaagg
     9001 gattttatgt ccaaaatgcg aacatattct tcagtatcgt ctcaatactt atgcgaatct
     9061 aggagacttt atttgtttaa attgtggctt caagagacca cagttggatt atcgattaac
     9121 tgaactcacg acgattacca atcacagttc tgagtttatc attgatggcc aagattacaa
     9181 aatcaatgtc ggtggcttat ataatattta caacgccctc gctgccgtct ctgttgctga
     9241 atttttcggt gtttctcccg agaaaatcaa ggcgggcttt aacaaaagta aggcggtttt
     9301 tggaagacaa gaaactttta ctattggtga taaatcatgc acccttatcc ttatcaagaa
     9361 tcctgttgga gccagtcaag cgcttgaaat gattcagcta gctgattacc catttagctt
     9421 atctgtgctt ttaaatgcta attacgctga cggcattgat accagctgga tttgggatgc
     9481 taattttgaa ctcatcaccc aaatgcccat tacagaaatt aatgctggcg gcgtccgcca
     9541 ctctgaaatt gctagaagac ttagagtcac aggatttgac gatactaaaa taaagcaagc
     9601 tgaaaagctc gagcaaatca tagaaaccat tgaaaaacaa gaagctaagc atgcctatat
     9661 tttagcaacc tatacggcaa tgttagaatt ccgtagctta cttgctgacc gccatgtcgt
     9721 tgaaaaggag atgatctaat gacatacact tcactaaaat ctcccgaaaa tcaagattac
     9781 atctacgacc tcactatcgc ccatctctat ggtaatctta tgaatactta tggtgataac
     9841 ggcaatattt taatgctcaa gtatgtcgct gaaaaacttg gagctagggt tactgttgat
     9901 attgtgtcta tcaatgatac ctttgaacaa gacgattacg atattgtttt ctttggtggt
     9961 ggtcaagatt atgaacaaag cattgttgcc aaggatttgc ctagcaaaaa agctgcctta
    10021 gcggattaca ttgctaataa taaagtggtg cttgccatct gtggtggctt ccaattacta
    10081 gggcaatact atgtccaagc taatggggtt aaaattgatg gacttggaat tatgggacac
    10141 tacaccctta atcagcacca aaatcgcttt attggagata ttaaaattca taatgacgaa
    10201 tttaacgaaa cctattatgg ctttgaaaat catcaaggac gtaccttctt atctggtgac
    10261 gaaaagccac ttggacgagt ggtttatggc aacggtaata ataaagaaga ccaaactgaa
    10321 ggtgttcact ataaaaacgt ctatggtagt tatttccacg gtcctatctt atcacgcaat
    10381 gttaatcttg cttaccgctt agtaacaaca gctcttaaaa aaaagtatgg atcagctatt
    10441 tcactatcta gctatgatga tatactgaaa caagaaatca ctgaagaata tgccgacctt
    10501 aaaagcaaag cttcctttaa taaagtttaa gttcttcttt catgtcatca aacaaaaact
    10561 cctaagcagg acacgatatt tcgcttagga gttttatagt tttttcttct gacagagctt
    10621 cttaagtgat taaaaccact aagaaacaat agctttagca acttcttcaa cggtcatgcg
    10681 tgagaagtac tgggtcgtat caatcgtttt taaacgttca aaaacatcac ggtactcata
    10741 tttacaacca attaataagt tctcaatgtc ttgcacatct ttgattccaa agaaatcacc
    10801 atagatttta aggtttttaa tgatagaatt ttcaacattt gcaaaggttg aaattttacc
    10861 tgcagggtaa cgaacattgc gtccaatcgt atattctggc gctttgccgt aggtccaatc
    10921 ccaactacca aattgctctt tggcagattg ttcaatctta gccaactcat cctctgacaa
    10981 gacatattct gtcatgtctg gatacgtttc tttcatcttg gtcaagattt tatctgaaaa
    11041 ttcctcaacg gtaatttttt ctggtaattc atttaaaata ttggtcacgc gagcacgaac
    11101 agacttcact cctttagact caatcttatc cttacttacc ttaagagcat cgccaagaac
    11161 ggtcatatcc acatcaaaca aaaggcaacc atggtgcatc atgcgacctt tgtagtaagc
    11221 ttgagcatta ccacagattt ttttgccatc aatttcaata tcattacggc ctgtaaagtt
    11281 ggctgttaca cccaaatcag ctaaagtcgc aatcacaggt tgtgagaagg ttttgaaatc
    11341 aaatgcgcct tccgctgttt tatttgaaat gatagtgtag ttcaagttat tcaaatcatg
    11401 ataaaccgca ccaccaccag ataaacgtcg cacaacatga ataccatgtt catcaatgta
    11461 ttctttattg atttcttgaa tggtgttttg atgtttgcca ataataatag ctggctcatt
    11521 gatccaaagg ataaacaatt cgtcttcttc cactagttct ctaaaagcat aggcttccaa
    11581 agcgatgtta aaggcaggat tgtgactttt gttaacgatg tatttcatag gagaaaaaat
    11641 cctttcaaaa atgtaatcta cctctctagt ataatgaaaa caaatataaa agacaagtgt
    11701 cctattctgt atgacatctt cagtacctac acttattagt gcacaaaaac tcagccatta
    11761 tttatgtaag ggtttataaa aatactttaa cgtggtatat ttttacttag taaaccaatc
    11821 tacttttatt atctcatttt tatattggag gaatgtatca gtgaatactt atttttgcac
    11881 acaccataaa caattactac tttattcaaa cctattcctt agctttgcta tgataggcca
    11941 aggaactgcc atttatgccg atacactgac ttcaaattca gaacctaata atacttactt
    12001 tcaaacgcaa acgctcacta ctacagatag cgaaaaaaag gtagtacagc cacaacaaaa
    12061 agactactat actgaattgt tagaccaatg gaacagtatt atcgcaggca acgatgctta
    12121 tgataaaacc aatcctgaca tggtcacttt tcataataaa gctgaaaagg atgctcaaaa
    12181 cattattaaa agctatcaag ggcctgacca cgaaaataga acttaccttt gggaacatgc
    12241 aaaggattat tccgcttcta ctaatatcac gaaaacttac cgcaatattg aaaaaatagc
    12301 aaaacagatc actaatcctg aatcatgcta ttatcaagat agtaaagcta ttgctattgt
    12361 aaaagacggt atggccttca tgtatgaaca cgcttataat ctagatcgtg aaaatcatca
    12421 aacaactgga aaagaaaaca aagaaaattg gtgggtttat gaaattggaa ctcctcgtgc
    12481 tattaataat accttatcct tgatgtatcc ttattttact caagaagaaa ttcttaaata
    12541 cacagctcca atcgaaaaat ttgtgcctga ccctactcgt tttagggttc gcgccgccaa
    12601 tttttcacct tttgaagcca atagcggaaa tttaattgat atggggcgtg ttaaactcat
    12661 ttccggtatt cttcgtaaag atgatctcga aattagtgat acaatcaaag caattgagaa
    12721 agttttcacg ctagttgacg aaggaaatgg tttttaccaa gacggttctt taattgatca
    12781 cgtggttact aacgctcaaa gtccacttta taaaaaaggc attgcttaca ctggagctta
    12841 cggtaatgtg cttatagatg gcttatcgca attaattcct attattcaaa aaacaaagtc
    12901 tcctatagaa gcggataaaa tggctactat ctatcattgg attaaccatt cttttttccc
    12961 tatcatcgtt cgtggagaaa tgatggatat gactcgaggg cgttctatca gtcgttttaa
    13021 tgctcaatct catgttgctg gcattgaagc acttcgtgct attttacgta ttgctgacat
    13081 gtctgaagag cctcaccgtt tggcacttaa aacacgtata aaaacactcg tcacacaagg
    13141 gaatgctttt tacaatgtct atgataattt gaaaacctat cacgatatca aacttatgaa
    13201 agaactacta aatgatactt ttgttccagt ccaaaaactt gatagttacg tagctagttt
    13261 caatagtatg gataaattgg cactatataa taataaacac gattttgctt ttggcctatc
    13321 aatgttttcg aatcgaactc aaaattatga agctatgaat aatgaaaatc ttcatggctg
    13381 gtttacttct gatggaatgt tttacctata caataacgat ttaggacact acagtgaaaa
    13441 ctattgggca acggtaaatc cctaccgctt acctggaacc acagaaactg agcaaaaacc
    13501 actagaggga actcctgaga atattaaaac gaactatcaa caagttggca tgactagtct
    13561 ctctgatgac gcttttgttg caagtaaaaa acttaataac acaagtgctc tagctgctat
    13621 gaccttcact aattggaata aaagcctcac tctcaataaa gggtggttta tcttaggaaa
    13681 caaaataatc tttgttggta gcaatatcaa aaaccagtca tctcacaagg cgtatacaac
    13741 tattgaacaa cgaaaagaaa atcaaaagca cccttaccat gcttatgtta acaatcaacc
    13801 cgttgacttg aataatcagc tagttgattt tacaaacact aaaagtattt tccttgaaag
    13861 tgatgatccc gctcaaaata ttggttacta cttcttcaag ccaacaacac ttagcataag
    13921 taaggcactt caaacagggc aatggcaaaa cataaaagct gatgacaaat caccagaagc
    13981 catcaaagaa gtttcaaata cctttatcac tatcatgcaa aaccatactc aagatggcga
    14041 tcgttatgcc tatatgatgc ttccaaatat gactcgtcaa gaatttgaaa cctatattag
    14101 caagcttgat atcgacttgt tagaaaacaa tgacaaactg gccgctgtct acgatcatga
    14161 tagtcaacag atgcacgtca ttcactatgg aaaaaaagca acgatgtttt caaatcataa
    14221 tctttctcat caaggctttt atagttttcc tcatcctgtc aagcaaaatc agcaataaaa
    14281 gcttgctcat caaggaattg cagctaaaaa caatgctctt aactctcata aaattaccca
    14341 caaaagacaa cgacgcttac cgcatactgg gtatcagttc tcctctttag aattcttagg
    14401 tggagcttta gttgcttctt ttaaccatat cacaaaaccc tttcgtaaga aagacttaag
    14461 gatttagctg ttattggaaa gtaagggtta acaatgaaac gttattaaag acaaaaaact
    14521 ggaaa
//
LOCUS       DAUCLR010000044        14300 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.044, whole genome shotgun sequence.
ACCESSION   DAUCLR010000044 DAUCLR010000000
VERSION     DAUCLR010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14300
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.044"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(114..1019)
                     /locus_tag="VGT87_001111"
     CDS             complement(114..1019)
                     /locus_tag="VGT87_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEQ3813971.1"
                     /translation="MRIQQLHYIIKIVECGSMNEAAKQLFITQPSLSNAVKDLEMEMG
                     ITIFNRNPKGITLTKDGVEFLSYARQIIEQTSLLEDRYKNHNTGRELFSVSSQHYAFV
                     VNAFVSLLKRTDMTRYELFLRETRTWEIIDDVKNFRSEIGVLFINDYNRDVLTKLFDD
                     NHLTASPLFKAQPHIFVSKSNPLATKSLLSMDDLRDFPYLSYDQGIHNSFYFSEEMMS
                     QMPHNKSIVVSDRATLFNLMIGLDGYTVASGILNSNLNGDQIVAIPLDVPDEIDIVFI
                     KHEKANLSKMGERFIEYLLEEVTFD"
     gene            1241..1777
                     /locus_tag="VGT87_001112"
     CDS             1241..1777
                     /locus_tag="VGT87_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813972.1"
                     /translation="MNVTEQNSHQILIQKLLVSIHYLTLFRDELKLVERTPSILGGEF
                     PAHLVQSELGDIVAAIDTLDMQQRLIESTFWYEESAFKLMNKTLDIVDNWIKGVDHLI
                     DLCQSKEVFQIIIGDKRIRVFGVLSDVFSSLKVSALSLKEAPIPDLLYEHIQRVNLEE
                     EAFIKYYQGPKVPTGEFE"
     gene            2054..2746
                     /gene="pyrF"
                     /locus_tag="VGT87_001113"
     CDS             2054..2746
                     /gene="pyrF"
                     /locus_tag="VGT87_001113"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEQ3813973.1"
                     /translation="MKEERPIIALDFSSFEETKAFLDLFPAEEKLYVKIGMELYYAQG
                     PDIVRSIKSLGHNVFLDLKLHDIPNTVRAAMAVLKELDIDMATVHAAGGVEMLKAARE
                     GLGQGPTLIAVTQLTSTSEDQMRGDQNIQTSLLESVLHYSKGAAKAQLDGVVCSAQEV
                     EAIKAVTPTGFTCLTPGIRPKGSNIGDQKRVMTPNQARRIGSDYIVVGRPITQAKDPV
                     AAYQAIKAEWAG"
     gene            2804..3433
                     /locus_tag="VGT87_001114"
     CDS             2804..3433
                     /locus_tag="VGT87_001114"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEQ3813974.1"
                     /translation="MTLASQIATQLLDIKAVYLKPEDPFTWASGIKSPIYTDNRVTLS
                     YPKTRDLIENGFVETIRAHFPEVEVIAGTATAGIPHGAIIADKMTLPFAYIRSKPKDH
                     GAGNQIEGRVLKGQKMVIIEDLISTGGSVLDAAAAASREGADVLGVVAIFTYELPKAS
                     QNFKEAGIKLITLSNYTELIAVAKLQGYITNDGLHLLKKFKEDQVNWQQ"
     gene            3630..5084
                     /locus_tag="VGT87_001115"
     CDS             3630..5084
                     /locus_tag="VGT87_001115"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEQ3813975.1"
                     /translation="MTYQDATAMAIAVQTGQTTPLELVTQAIYKAKKLNPTLNAITSE
                     RFEAALEEAKQRDFSGKPFAGVPIFLKDLGQELKGHSSTSGSRLFKEYQATKTDLFVK
                     RLEALGFIILGRSNTPEFGFKNISDSSLHGPVNLPKDNTRNAGGSSGGAAALVSSGIS
                     ALATASDGGGSIRIPASFNGLIGLKPSRGRMPVGPGSYRSWQGASVHFALTKSVRDTR
                     NLLYYLQMEQMESPFPLATLTKDSIYQSLQRSLTIGFYQRLSDGSPVSLDTAKALRQA
                     VTWLREQGHQLVELEEFPVNMTEVIRHYYIMNSVETAAMFADIEDTFGRPMTKDDMET
                     MTWAIYQSGKDIPAWRYSQVLQKWDTYSATMASFHETYDLLLTFTTNTPAPKHGELVP
                     DSKLMANLAQAEIFSSEEQFNLVETMFGKSLAINPYTALPNLTGQPAISLPTYETKEG
                     LPMGIQLIAAKGREDLLLGIAEQFEAAGLLKIPQ"
     gene            5202..6065
                     /locus_tag="VGT87_001116"
     CDS             5202..6065
                     /locus_tag="VGT87_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEQ3813976.1"
                     /translation="MKKSVTLLSIGLASLLLAACAPHKSQKSSWDTIKEKGVLKVATP
                     GTYQPTSFYNDNNELVGYEVDMVKEIGKRLNIKVKFVETGFDQAFTSVDSGRVDISLN
                     NFDITPKRQKKYNISTPYKYGVGGMIVRADGSSNMVKKDLSDWKGKKAAGASGTEYMK
                     VAQKQGAELVTYDNVTGDVYLNDVANGRTDFIPNDYPAQKLFVDYMLSQNPNLNVKMS
                     DVQYNPTEQGIVMNKKDDSLKKKIDAVIKDMIKDGSLKKISETYYAGQDLTKPFGKDK
                     KIPVIDTKDVN"
     gene            6094..6744
                     /locus_tag="VGT87_001117"
     CDS             6094..6744
                     /locus_tag="VGT87_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEQ3813977.1"
                     /translation="MINIPLMKDSLGFVLSGLPYTLGISLLSFFTGLFLGLGLALLGR
                     SHQPLLHYLVRAYISIMRGVPMIVVLFVLYFGLPYYGLELPALLCAYLGFSMVSAAYI
                     SEVFRSSIEAIDKGQWEAAKALGLPYALMVKKIILPQAFRIAVPPLGNVIIDMVKSSS
                     LAAMITVPDIFQNAKIIGGREWDYMSMYILVAFIYWIIAFLLERYQEFLENKLALV"
     gene            6877..7530
                     /locus_tag="VGT87_001118"
     CDS             6877..7530
                     /locus_tag="VGT87_001118"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEQ3813978.1"
                     /translation="MAHSIWHEKIKSFLPEHYYGRINHFLDEAYASGLVYPPRENVFK
                     ALQVTPLEETKVLILGQDPYHGPKQAQGLSFSVPEEISAPPSLINILKELADDIGPRD
                     HHDLSTWASQGVLLLNACLTVPAGQANGHAGLIWEPFTDAVIKVLNEKDSPVVFILWG
                     AYARKKKAFITNPKHHIIESSHPSPLSSYRGFFGSKPFSRTNAILEKEGMTGVDWLK"
     gene            7662..8930
                     /locus_tag="VGT87_001119"
     CDS             7662..8930
                     /locus_tag="VGT87_001119"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEQ3813979.1"
                     /translation="MILIKNGRVMDPKSQRDQVADVLIDGKQIVKIASAIECQEAQVI
                     DASGLIVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGVTTVVMMANTNPVISDVE
                     TLQEVLASAAKEKIHIYTNASVTQAFNGKDVTDFKALLEAGAVSFSDDGIPLESSKVL
                     KEAFDLANANQTFISLHEEDPQLNGVLGFNEGIAEEHFHFCGATGVAEYSMIARDVMI
                     AYDRQAHVHIQHLSKAESVQVVAFAQQLGAKVTAEVSPQHFSTTEDLLLIAGTSAKMN
                     PPLRTQRDRLAVIEGLKSGVITVIATDHAPHHKDEKAVDDMTKAPSGMTGLETSLSLG
                     LTHLVEPGHLTLMSLLEKMTLNPALLYGFDAGYLAENGPADLVIFADKQERLITENFA
                     SKASNSPFIGNKLKGVVKYTIADGEVVYPN"
     gene            complement(8988..9629)
                     /gene="plsY"
                     /locus_tag="VGT87_001120"
     CDS             complement(8988..9629)
                     /gene="plsY"
                     /locus_tag="VGT87_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEQ3813980.1"
                     /translation="MKLLLFITIAYLLGSIPTGLWIGQYFYHINLREHGSGNTGTTNT
                     FRILGVKAGTATLAIDMFKGTLSILLPIIFGMTSISSIAIGFFAVLGHTFPIFANFKG
                     GKAVATSAGVLLGFAPLYLFFLASIFVLVLYLFSMISLASVVSAIVGVLSVLTFPAIH
                     FLLPNYDYFLTFIVILLAFIIIIRHKDNISRIKHHTENLIPWGLNLSKQVPKK"
     gene            9764..11713
                     /gene="parE"
                     /locus_tag="VGT87_001121"
     CDS             9764..11713
                     /gene="parE"
                     /locus_tag="VGT87_001121"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEQ3813981.1"
                     /translation="MTKKEITINNYNDDAIQVLEGLDAVRKRPGMYIGSTDATGLHHL
                     IWEIVDNAVDEVLSGFGDDIKVVINKDGSVSVADSGRGMPTGQHAMGIPTVQVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSAWLEVEITRDGAVYRQRFENGGKPVTTLK
                     KVGTAPKSKSGTVVTFMPDDKIFSTIDFKFNTIAERLKESAFLLKNVKMSLTDLRGDE
                     PIVEEFHYENGVQDFVEYLNEDKETLTPVIYMEGQDHDFQVEVALQYNDGFSDNILSF
                     VNNVRTKDGGSHETGLKSAITKAMNDYARKTNLLKEKDKNLEGSDYREGLSAVLSILV
                     PEQHLQFEGQTKDKLGSPLARPIVDSIVSEKLTFFLLENGEVASHLVRKAIKARDARE
                     AARKARDDSRNGKKNKKDKGLLSGKLTPAQSKNAKKNELYLVEGDSAGGSAKQGRDRK
                     FQAILPLRGKVLNTEKAKMADILKNEEINTMVYTIGAGVGADFNLEDINYDKIIIMTD
                     ADTDGAHIQTLLLTFFYRYMRPLVETGHVYIALPPLYKMSKGKGKTEKIAYAWTDGEL
                     EDLRREFGKGAILQRYKGLGEMNANQLWETTMDPETRTLIRVTIDDLARAERRVSVLM
                     GDKAAPRRQWIEDNVKFTLEENTVF"
     gene            11804..14263
                     /gene="parC"
                     /locus_tag="VGT87_001122"
     CDS             11804..14263
                     /gene="parC"
                     /locus_tag="VGT87_001122"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021340535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEQ3813982.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQERALPDIRDGLKPVQRRI
                     LYSMNKDGNTFEKGYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQDWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKNTVSFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSSGISAGYATDIPPHNLSEVIDAVVYMIDHPKASLEKLMEFLPGPDFPPGGI
                     IQGADEIKKAYETGKGRVVVRSRTEIEELKGGKQQIIVTEIPYEVNKAVLVKKIDDVR
                     VNNKVPGIVEVRDESDRTGLRIAIELKKEADSQTILNYLLKYTDLQVNYNFNMVAIDH
                     FTPRQVGLQKILSSYISHRKDIIIERSKFDKAKAEKRLHIVEGLIRVLSILDEIIALI
                     RSSDNKADAKENLKVSYDFSEEQAEAIVTLQLYRLTNTDIVTLQNEENDLRDLITTLS
                     AIIGDEATMYNVMKRELREVKKKFANPRLSELQAESQIIEIDTASLIAEEETFVSVTR
                     GGYLKRTSPRSFNASSLEEVGKRDDDELIFVKQAKTTEHLLLFTTLGNVIYRPIHELT
                     DLRWKDIGEHLSQTISNFATEEEILYADIVTSFDQGLYVAVTQNGFIKRFDRKELSPW
                     RTYKSKSTKYVKLKDDKDRVVTLSPVIMEDLLLVTKNGYALRFSSQEVPIQGLKSAGV
                     KGINLKNDDSLASAFAVTSNSFFVLTQRGSLKRMAVDDIPQTSRANRGLLVLRELKTK
                     PHRVFLAGGVQSDTSAEQFDLFTDIPEEETNQQMLKVISKTGQTYEIALETLSLSERT
                     SNGSFISDTISDQEVLVARTR"
BASE COUNT         4537 a         2572 c         3005 g         4186 t
ORIGIN      
        1 ggagttgagc ctttggagac aatctattgt ctgatattta tcaaaaaaca cgataacttg
       61 accgatacag gtcttactac cgtgtttttt tagtatgacg attgaaaaag acattaatca
      121 aaggttactt cttcgagaag gtattcgata aaacgttccc ccattttgga caagttggct
      181 ttttcatgct tgataaaaac aatgtcaatt tcgtcaggga catctagcgg gatagccaca
      241 atctggtcac catttagatt gctatttaga ataccactgg caacggtgta gccatctaaa
      301 ccaatcatta agttaaagag ggtggcacgg tcactgacca caattgattt gttgtggggc
      361 atctgggaca tcatctcttc tgaaaaataa aaggagttgt gaatcccttg gtcatagctc
      421 aggtaaggaa aatctctcaa gtcgtccatg cttaataggc ttttagttgc tagaggattt
      481 gatttgctga caaaaatatg aggctgtgcc ttaaataatg gagaggcagt caaatgattg
      541 tcatcaaata atttggtcaa gacatctcgg ttatagtcgt tgataaagag aacaccaatt
      601 tcagatcgaa agtttttgac atcatcaatg atttcccaag ttcttgtctc gcgcaaaaag
      661 agttcgtacc gagtcatgtc ggtccgcttg aggagtgaga caaatgcatt gactacgaag
      721 gcataatgtt gagatgaaac gctaaagagc tctcggccag tattgtggtt tttatagcgg
      781 tcttctaaca gtgatgtttg ctctataatt tggcgagcat aagataaaaa ttccacccca
      841 tcttttgtca aggtgatccc tttaggattt cggttgaaaa tggtaatgcc catctccatt
      901 tccaaatcct tgaccgcatt ggatagactt ggttgtgtga taaataattg cttagcagct
      961 tcattcatag agccgcactc aacaattttg ataatataat gcagttgttg aattctcata
     1021 agtacagtct agctcaaaaa aaataaagac gcaagcacaa acgtcatcat gggtttagaa
     1081 ctttgaaaaa gcgctggagt cttttctcaa tatggcctag gataaatatg attcaatttt
     1141 gagagttgaa tctatcaatt tcattgccct tttatttttc ttgtgttata ttaatagtat
     1201 taacaaatag gtatttttct aatcaaaggg tggtggtcgt gtgaatgtta ctgaacaaaa
     1261 tagtcatcaa atcttaatcc aaaaattatt ggtcagtatc cactatttga ctctttttcg
     1321 agatgaactg aagcttgtag aacgaactcc ttctatactt ggaggggaat ttccagctca
     1381 tttagtacag tctgagttgg gagatattgt tgcggccatt gataccttgg atatgcagca
     1441 acgcttgatt gagtctacct tttggtatga agaatcagct tttaagttga tgaacaaaac
     1501 tttggatatt gtcgataatt ggattaaagg agtggatcat ctgatagatt tatgccagtc
     1561 caaagaagtc tttcaaatta ttattggaga taaacgcatt cgagtttttg gggtcttaag
     1621 tgatgttttt tcatctttaa aagtgagtgc tttgtcactt aaggaagctc ctattccgga
     1681 tttgctctat gaacatatcc agagggttaa tcttgaagaa gaagctttta taaaatatta
     1741 ccaaggccct aaggtaccca caggtgagtt tgaataaatg attgggtaaa aatgataaaa
     1801 tgagttgacg taagtgtaca aaaaaagata tagtaggaaa aatgtgttca aataacaaaa
     1861 agttttgaca tcttgtttta aaaaagctat aatagtttaa atttagacac ctttaagtga
     1921 gtccagagag gcaaacaagg tatacaagat aaaaaaaggg aattcccttt tagtttgctg
     1981 tagcctcgct tctttggtag cgaggctttt tggtacttaa tgagatattg acacattata
     2041 gaaaggataa gttatgaaag aagaacgccc aattattgct ttagactttt catcatttga
     2101 ggagactaaa gcatttttag acttatttcc agcagaagaa aaactatacg tgaaaattgg
     2161 tatggaatta tattatgcgc aaggaccaga tattgttcgg tctatcaaat cgttagggca
     2221 taatgtattt ttggatttaa aattacatga tattccaaat actgttcgtg ctgctatggc
     2281 agttcttaaa gaattagata ttgatatggc aactgttcat gcagctggtg gtgtagagat
     2341 gctaaaagca gctcgagaag gactaggcca aggccctacc ttaattgcgg taacgcagct
     2401 aacttcaacc agtgaagacc aaatgcgtgg tgatcaaaat attcaaacga gtcttttaga
     2461 gtctgttttg cattacagca agggggcagc taaagctcag ctagatgggg tggtttgttc
     2521 ggcacaagaa gtagaagcta tcaaggcggt gacgcctacg ggatttactt gcttaactcc
     2581 tggcattcgt cctaaaggca gtaatattgg cgatcaaaaa cgagtgatga ctccaaacca
     2641 agcaagacgc attggaagtg attatattgt ggtagggcga ccaattaccc aggcaaaaga
     2701 cccagtggct gcttaccaag ccataaaagc tgagtgggca ggctaacaga taagattagg
     2761 aaaccaacac ttgtctgtta aagaagaaaa aggagaataa gaaatgacac tagcaagcca
     2821 aattgcaact caattacttg atattaaagc tgtttattta aagcctgaag atccttttac
     2881 gtgggcatct ggcattaaat cacctattta cactgataat cgtgtcaccc tttcttatcc
     2941 taagactcgt gatttaattg agaatgggtt tgtggaaact atcagggctc atttcccaga
     3001 agttgaggtc atcgctggta cagctacagc aggtattcct cacggtgcca ttatagcaga
     3061 taaaatgact cttccttttg cttatattcg cagtaagcca aaagatcatg gagcaggcaa
     3121 ccagattgag gggcgtgtgc ttaaaggcca aaaaatggtt atcattgaag atttgatttc
     3181 aacaggtggt tcggtactag atgcagccgc agcagccagt cgtgagggag ctgacgttct
     3241 gggagttgtt gcgattttca cttatgagtt accaaaagct agtcagaatt ttaaagaagc
     3301 tggtatcaag ttgattacgc tttctaatta tactgagcta attgcagttg caaagttgca
     3361 aggctatatt actaatgatg gtttacattt acttaaaaaa tttaaagaag accaggtaaa
     3421 ctggcaacaa taaggaacta tcagccaggt ttatttttaa tgaaaaaatc tctttaaccc
     3481 caattcaaaa tgttatgttt tgattgtttt gtctacattc cttacacatc acattctaat
     3541 ttctaacctt ttaatggtgg ttttctttta tttgtgtttt aataaaaaag aatgttttag
     3601 gattcatatc ttagaaagga agagtagcta tgacttatca agatgcgacg gctatggcta
     3661 ttgctgttca gaccggtcag accacaccac tagaattagt aacccaggct atttataagg
     3721 ctaaaaagtt gaaccctacc ttaaatgcta ttacctcaga acgctttgaa gcagccctag
     3781 aagaagccaa acaaagggat ttttcaggta agccttttgc aggagttccc atttttttga
     3841 aagatttggg acaagaatta aaaggtcact catcaacctc aggttccaga ttgtttaaag
     3901 agtatcaagc gactaaaacg gatttatttg tgaaaagatt agaagcttta ggctttatta
     3961 ttttagggcg ttccaacacg cctgaatttg gctttaaaaa tattagcgat agcagtttgc
     4021 acggaccagt gaatttacca aaggataata cacgaaatgc tggtggctca tctgggggag
     4081 ctgctgctct agtgtcatca ggaattagtg cactagcaac tgctagtgat ggtggtggtt
     4141 ctattcgaat tccagcctcg tttaatggct taattgggct caaacctagc cgtgggcgca
     4201 tgcctgtcgg acctggttct tatcgaagct ggcaaggagc ctcagttcat tttgccttga
     4261 ccaaatccgt tagagatact agaaatctac tctactattt acagatggaa caaatggaaa
     4321 gccccttccc cctagccacc ttaacaaaag atagtattta tcaatcgcta caacgttcct
     4381 tgacaattgg tttttatcaa cggttaagtg acggtagccc agtatcttta gatactgcaa
     4441 aagctttaag acaagcagta acttggctaa gagaacaagg gcatcagcta gttgaattgg
     4501 aagagtttcc tgtaaatatg acagaagtga ttcgtcatta ttatattatg aatagtgtgg
     4561 aaacggctgc tatgtttgct gatattgaag acacttttgg acgtccgatg accaaagacg
     4621 atatggaaac catgacctgg gctatttacc aatcaggtaa agacatccca gcttggcgtt
     4681 actcacaggt ccttcaaaaa tgggatacgt acagtgcaac catggctagt tttcatgaga
     4741 cttatgacct gttgttgacg ttcacaacca acacaccagc tcctaagcac ggagaacttg
     4801 tcccagattc gaaactcatg gctaacttgg ctcaagctga aattttcagc agtgaagagc
     4861 aattcaattt ggtcgaaacc atgtttggta agagtctggc tattaatcct tacacagctc
     4921 tgcctaatct aactggccag ccagctatta gtttacctac ttacgaaact aaggaagggc
     4981 tcccaatggg cattcaacta attgcagcca aaggtagaga ggacctatta ttaggaattg
     5041 cagagcagtt tgaggcagca ggattattga agattcctca ataataatct tataaaacag
     5101 tttaactatt atagattaac tttttaaaga gattgagcgc tcactgataa cacaacttaa
     5161 tcaaaatgtt atactattct tatcttaata aaggagttac tatgaagaaa tcagttactt
     5221 tactgtctat cggacttgcc agccttttat tggcagcatg tgcgccacat aaatctcaaa
     5281 aatcaagttg ggacactatc aaagaaaaag gggtgttaaa agtagctacc ccaggaacct
     5341 atcagccaac ttctttttac aacgataata atgagttagt aggttacgaa gtagatatgg
     5401 tcaaagaaat cggtaaacga cttaacatta aagtcaagtt tgttgaaaca ggatttgacc
     5461 aagcgtttac ctcagttgat agtggtcgag tggatatttc tttgaacaac tttgacatta
     5521 ccccaaaacg tcagaaaaaa tacaatatct ctacgcctta taaatacggg gtaggaggca
     5581 tgattgtccg tgctgatggc agctcaaaca tggttaaaaa agatcttagc gactggaaag
     5641 gaaaaaaagc agctggtgct tctggaacag aatacatgaa agtagcgcaa aaacagggag
     5701 ctgaactggt gacctacgat aatgttacag gagatgttta tctaaacgat gtggctaacg
     5761 gtcgaacaga tttcattcca aatgattacc cagctcaaaa attatttgtg gattacatgt
     5821 tatctcaaaa tccaaatcta aatgtcaaaa tgagcgatgt tcagtacaac ccaaccgaac
     5881 aaggaattgt tatgaacaaa aaagacgata gcctcaagaa aaaaatagat gctgttatta
     5941 aagacatgat aaaagatggt agcttgaaga aaatctctga aacctactat gctggtcaag
     6001 atttgacaaa accttttggc aaagacaaaa aaattcccgt cattgatacg aaagacgtta
     6061 actaatactt gatgttaaag gagtcctaac cacatgatta atatcccatt aatgaaagat
     6121 agtttaggat ttgtgttatc gggattacct tatacccttg gtatttcgtt gctcagtttt
     6181 tttacgggtc tgtttttagg tttaggccta gcccttctgg gaaggtcaca tcagccactg
     6241 ctacactatc ttgttagggc ctacatctct attatgcgag gtgtgccgat gatcgtggtg
     6301 ctctttgttc tttatttcgg gttgccttat tatggtttag aattgccagc tttactttgc
     6361 gcttatcttg gtttttctat ggttagcgct gcctatattt ctgaagtttt tcgctcttct
     6421 atagaagcta ttgacaaggg acagtgggaa gcagctaagg ctttagggtt gccatatgct
     6481 cttatggtta agaaaattat tcttcctcaa gcctttagaa ttgcagttcc tcctctaggc
     6541 aatgtcatta ttgatatggt taaaagttcg tcgctagctg ccatgattac cgtaccagat
     6601 atttttcaaa atgccaaaat tatcggtggt agggagtggg actacatgtc catgtacatt
     6661 ttagtagcct ttatctattg gattattgct tttttgttag aacgttacca agaattctta
     6721 gaaaataagc tagcacttgt ttaatatcct gtaaaaaacg aatattaacc ctggaaatat
     6781 catattttca gggtctttct atttaaaatg tggtaaacta gatagaaaag gacatcgtat
     6841 ttaacttaga aactgacgtg attagaaagg aatcttatgg ctcattcaat ttggcatgag
     6901 aaaatcaaat catttttgcc ggagcattac tacggtcgca tcaatcactt tttagatgaa
     6961 gcctatgctt ccggtcttgt ctaccctcca cgagaaaatg tctttaaagc cttacaagtc
     7021 actcctttgg aagaaaccaa agtgttgatt ttaggacaag acccttatca tggccccaaa
     7081 caggcccagg gcttatcatt ttcagttcca gaagagattt ctgctccgcc atcccttatt
     7141 aatattttaa aagaattagc agatgacatt ggtcctcgtg accatcatga tttaagcact
     7201 tgggctagtc aaggggttct gcttttgaat gcttgtttaa cagtgccagc aggacaggct
     7261 aatgggcatg caggcttaat atgggagcca tttacagacg ctgttattaa agtgctgaat
     7321 gagaaagaca gccctgtagt tttcattttg tggggagctt atgcaaggaa gaaaaaagcc
     7381 ttcattacta atccaaagca ccacatcatc gagagctctc atccaagccc tttgtcatct
     7441 tatcgtggct tttttggcag caaacccttt tcaagaacca acgctatttt agaaaaagag
     7501 ggcatgactg gcgtagattg gctaaaataa gccagccatt tcaactaaat agcttaagct
     7561 agacattttg atacctttaa taagtgaaat gagtgcagta gatttttcct attaaagaac
     7621 gtaaggtctt atggttaaaa cagataaata aaggagaaaa catgatactc atcaaaaatg
     7681 gacgcgttat ggacccgaaa tcgcagagag atcaagtggc agatgtcttg attgatggta
     7741 aacagattgt aaagatagct tcagctattg aatgccaaga agctcaagtg attgatgcta
     7801 gtggtcttat tgttgccccg ggtttggtcg atattcatgt gcattttaga gaacccggtc
     7861 aaacgcacaa agaagatatt cacacgggag ccttggcagc agcagcaggt ggtgtgacta
     7921 cggttgtcat gatggctaat accaatcctg tcatttcaga tgtcgaaact ttacaggaag
     7981 tgctagctag cgcagctaaa gaaaagattc atatttacac caatgccagt gtcacccaag
     8041 cctttaatgg aaaagacgtg actgatttta aagctttgtt ggaagcagga gcagtaagct
     8101 tttcagatga tggtattccc ttagaaagtt ctaaagtttt aaaagaagcc tttgatttgg
     8161 ctaatgccaa ccaaactttc atttctttgc atgaggaaga tcctcagtta aatggggttc
     8221 ttggttttaa tgaaggtatt gctgaggagc attttcattt ttgtggggca actggtgtag
     8281 cagaatatag tatgattgca agagatgtca tgattgctta tgacaggcaa gctcatgttc
     8341 atatccaaca tttatctaaa gcagaatcgg ttcaagtagt ggcttttgct cagcagttgg
     8401 gtgctaaggt aactgctgaa gtgtccccac aacatttttc aacaactgaa gatctcttat
     8461 tgatcgcagg taccagcgcc aagatgaatc ctccccttcg tacacaaaga gatcgcttag
     8521 ctgtcataga aggcttgaaa tcgggtgtta tcacagtcat tgccactgat catgctcctc
     8581 atcacaagga tgaaaaagct gtggatgata tgactaaagc cccatctggt atgacaggac
     8641 ttgaaacttc tttgtcatta ggtttgacgc atttggttga accaggtcat cttacactta
     8701 tgtcactatt agaaaaaatg acgcttaatc cagctttgct ttatggtttt gatgcgggtt
     8761 atttggcaga aaatggtcca gcagatttgg ttatttttgc agacaagcaa gaacgtctga
     8821 tcactgagaa ttttgcttct aaagctagta attccccatt tattggtaac aaactcaaag
     8881 gtgtcgtgaa atacactatt gcagacggag aggttgttta ccctaattaa caaatgagtt
     8941 aaaacaaaaa agcttgactt tcagagaaag tcaggctttt agtaaattta ttttttaggc
     9001 acttgtttgc ttaaatttag cccccaaggt attagatttt cagtatgatg tttgatacga
     9061 ctaatgttat ctttgtgtct tataataata atgaacgcaa gtaatatcac aataaaagtt
     9121 aaaaagtagt cataatttgg taaaagaaaa tgaatggcag gaaatgttaa aacagataac
     9181 acaccaacga tagctgaaac cacactagct aaagatatca tactaaataa atagagaact
     9241 aaaacaaaga tagatgctaa aaaaaagaga tataacggag caaagcctag caatacacca
     9301 gcacttgttg ctacggcctt accaccttta aagttggcaa aaataggaaa agtatgccct
     9361 aaaactgcga aaaagccgat agcaatggat gaaattgaag tcataccaaa aataattggt
     9421 aacaatattg aaagtgtccc tttaaacata tcaatagcta aggtagctgt tcctgccttg
     9481 acacctaaaa tccgaaaagt atttgtggtt ccagtatttc ctgatccatg ctctcgtaag
     9541 ttgatgtggt aaaagtactg tccaatccat agtccagttg gaatagaacc aagtaaatag
     9601 gcaatggtaa taaaaagtag taatttcata atccttatta taacatattc atagattgga
     9661 gaactagatc tggaaaagaa ttcggaaaaa attagcaaat tattttgcaa aattggtaaa
     9721 aaacttgtaa gataaaaaag atgaaactgt ctggaggtcg gtgttgacta agaaagaaat
     9781 tacaattaat aattataatg acgatgctat ccaagtccta gaagggttag atgctgttcg
     9841 caagcgtcca gggatgtata tcggttcaac agatgctacg ggattgcacc acctgatttg
     9901 ggaaattgtt gataatgctg tcgatgaggt cttgtctggt tttggagatg atattaaggt
     9961 tgttatcaat aaagatggct cggtcagtgt ggcagatagc ggacgtggaa tgccaacagg
    10021 tcagcatgcc atggggattc ccaccgttca agttattttt acgattcttc atgcaggtgg
    10081 taaatttggt cagggtggct acaaaacgtc aggtggtttg cacggtgttg gctcctctgt
    10141 tgtcaatgct ttgtctgcct ggttagaagt cgaaatcaca agagacgggg cagtgtatcg
    10201 tcagcgcttt gaaaatggtg ggaaacctgt tacaaccctt aaaaaggttg gtacagctcc
    10261 taagtctaaa tcaggaactg tcgtcacctt tatgcctgat gataagattt tttcaaccat
    10321 tgactttaag ttcaatacca ttgcagaacg gcttaaagaa tctgcttttc ttttgaaaaa
    10381 cgttaagatg agcttgacag atcttcgtgg agacgaaccg attgttgaag agtttcatta
    10441 tgaaaatggg gttcaagatt tcgtcgaata cctcaatgaa gataaggaaa ccttgacacc
    10501 tgttatttac atggaaggtc aagaccacga ctttcaagtc gaggttgctt tgcagtataa
    10561 cgatggtttc tccgacaata tcttatcctt tgtaaacaat gtgcgcacca aagatggtgg
    10621 aagccatgaa acgggactga agtcagctat taccaaagcg atgaatgact atgctcgtaa
    10681 aaccaatctc ttaaaagaaa aggataaaaa tctagagggc tcagattatc gagaagggct
    10741 ctcagcagta ttatctatct tagttcctga gcagcacttg caatttgaag gacagaccaa
    10801 agacaaattg ggaagtccct tagcgagacc gattgtcgat agtatcgttt ctgaaaaatt
    10861 aaccttcttt ttactcgaaa atggagaagt agcttctcat ctagttcgca aagccattaa
    10921 agctagagat gctcgcgaag ctgctcgaaa agcgcgtgat gactcacgca atggtaagaa
    10981 aaacaaaaaa gacaagggcc tactatccgg gaaattgact cctgcccagt ccaaaaatgc
    11041 caagaaaaat gaattgtatc tggtcgaagg agattctgcg ggaggttcag ccaaacaagg
    11101 acgagaccgc aaatttcagg ctattttacc attacgtggc aaagttttaa ataccgaaaa
    11161 agctaaaatg gctgacatct tgaaaaatga agaaattaac actatggttt ataccattgg
    11221 agctggagtg ggagctgact ttaatctcga ggatatcaac tatgataaga ttattatcat
    11281 gaccgatgcc gataccgatg gtgcccacat tcaaaccctc ttattgacct tcttttaccg
    11341 ttatatgcga ccattagtag aaacaggtca cgtttatatt gcgcttcccc cgctttacaa
    11401 aatgtcaaaa ggtaaaggca aaactgaaaa aattgcttat gcttggactg atggtgaatt
    11461 ggaagatctg cgcagagaat ttggcaaggg ggctattctt caacgctata aaggactggg
    11521 tgaaatgaat gctaaccaat tatgggaaac cactatggac cctgaaacca gaacccttat
    11581 tcgtgttact attgacgact tggcaagagc agagcgacgt gtttctgtgt tgatgggtga
    11641 caaagcagcc ccacgccgtc agtggatcga ggataacgtc aagtttacct tagaagaaaa
    11701 tacggtgttt tagtcataca agtttctcac agataattac aagtgctaaa gacgattgtt
    11761 ttttaggtaa ttaataaata atgaaaaaga aagctatttg aatatgtcaa acattcaaaa
    11821 catgtccctt gaggatatta tgggagaacg cttcggccgt tattccaagt atattattca
    11881 ggaacgggca cttcctgata ttcgagatgg attgaaaccc gttcagcgtc ggattcttta
    11941 ttcgatgaat aaggatggca atacttttga aaaaggttac cgtaaatcag ccaaatcagt
    12001 tggtaatatt atggggaatt tccacccaca tggggattcc tctatttatg atgccatggt
    12061 acggatgagt caagattgga agaaccgtga aatccttgtt gaaatgcacg gaaataatgg
    12121 ttcaatggat ggtgatccgc ctgcggctat gcgttataca gaagctcgct tgtctgaaat
    12181 tgcgggttat cttcttcaag acattgagaa aaatacggtc tcttttgctt ggaactttga
    12241 tgacacagaa aaagaaccca ccgttttacc agcagctttt cctaacttac ttgtcaatgg
    12301 gtcttctggt atttccgcag gctatgcgac agatattcca ccgcacaatt tgtcagaagt
    12361 gattgatgct gttgtgtata tgattgatca tcccaaagct agtttagaga aattaatgga
    12421 gtttttacca ggaccagatt tcccgccagg aggcattatc caaggagctg acgaaatcaa
    12481 aaaagcctac gaaacaggta agggacgtgt cgttgttcgc tctaggacag aaatcgagga
    12541 acttaaaggt ggtaaacaac aaattattgt cactgagatt ccttatgaag ttaacaaagc
    12601 agtcttggtc aaaaagattg acgatgtgcg tgttaacaat aaagtcccag gcatcgtaga
    12661 agtacgcgat gaatctgatc gcacaggtct tcgcatagct attgagttga aaaaagaggc
    12721 tgatagccaa accattctaa attatttgtt gaaatacaca gaccttcaag ttaattataa
    12781 ttttaacatg gttgctattg accacttcac accacgtcaa gtaggacttc aaaaaatact
    12841 atcaagctat atcagtcacc gtaaagatat tattattgag cggtctaaat tcgataaagc
    12901 caaagctgaa aaacgcctcc acattgttga aggattgatt cgtgtcttgt ccatcttaga
    12961 tgagattatt gctttgatta gatcgtctga caataaggct gatgctaaag aaaatctgaa
    13021 agtcagctat gatttctctg aggaacaagc agaagcgatt gtaaccttgc agctttatcg
    13081 tttaactaat accgatattg ttactctcca aaatgaagaa aatgatttaa gagacttaat
    13141 cacgaccctt tccgctatta tcggtgacga agcaaccatg tataacgtca tgaaacgtga
    13201 attaagagag gtgaaaaaga aattcgccaa tcctcgttta tcagagttac aagcggaaag
    13261 tcaaatcatt gagattgaca ctgccagctt gattgcagag gaagaaactt ttgttagtgt
    13321 gacacggggc ggatacctca aaagaaccag cccacgatcc ttcaatgcgt ctagtttaga
    13381 agaagttggc aagagagatg atgacgaact catcttcgtt aaacaggcca aaacaacaga
    13441 acaccttttg ttgtttacaa ctttaggcaa tgtcatttac cgaccaatcc atgaattgac
    13501 cgatttacgt tggaaggata tcggtgaaca tttatcacaa accatttcaa acttcgcgac
    13561 agaagaagag attctctacg ctgatattgt gacttccttt gaccagggct tgtatgtggc
    13621 tgtcacacaa aatggtttta tcaaacgctt tgatcgtaaa gaactaagtc cttggcgaac
    13681 ttataaatct aaatcaacca aatatgtgaa gctaaaggat gacaaagacc gcgtggttac
    13741 cttgtcacca gttattatgg aagacctcct attagtgact aaaaatggtt atgctttgcg
    13801 attctccagt caggaagtgc ctattcaagg gttgaaatct gctggtgtta aaggcattaa
    13861 cttgaaaaat gatgacagcc ttgcctcagc ctttgctgtg acaagtaact ctttctttgt
    13921 cttaactcag aggggttctc tcaaacggat ggctgtcgat gatattcctc agacaagtcg
    13981 tgccaatcgt ggactattgg tcctacgaga gttaaaaacc aaacctcata gagtttttct
    14041 ggccggaggc gttcaatcag atacttcagc tgagcagttt gatttgttca ctgatatacc
    14101 tgaagaagaa actaatcaac aaatgcttaa agtcattagt aagactggtc aaacctacga
    14161 gattgcttta gaaacgcttt cgttatcgga aagaaccagc aatggctcct ttatttcaga
    14221 cactatttcg gatcaagaag tactggtagc tagaacacgt taaaaagagt gaaaactctt
    14281 tttaatttta tggtaaattt
//
LOCUS       DAUCLR010000045        14153 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.045, whole genome shotgun sequence.
ACCESSION   DAUCLR010000045 DAUCLR010000000
VERSION     DAUCLR010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14153
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.045"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..459)
                     /locus_tag="VGT87_001123"
     CDS             complement(<1..459)
                     /locus_tag="VGT87_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813983.1"
                     /translation="MTTREIAVTIWIIVLLILVFYFCIKKGIFKSVLDILISIWIVLK
                     LPISQWVSVANIFYIVLIYYVTKNDIELSYWYIKDYVIIFLFTIFPAILLLKESSVVE
                     IIRNQWRELLMFNTALLFISNTYTFSLPIELLLVFLLIILSIFSAVIDTKK"
     gene            complement(553..993)
                     /locus_tag="VGT87_001124"
     CDS             complement(553..993)
                     /locus_tag="VGT87_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEQ3813984.1"
                     /translation="MTSSTQACVFSGVKIALMQDQHLVTILRDNKSTIPFPDTWDLPG
                     GGREGLETPFECVQREVMEELGIAISQDMVVWEKAYQGVMNPETYSIFMVAMISKDLV
                     KAIHFGEEGQAYKFVPVKDFLADKKAVPQLQERLAAYLAEKSDQ"
     gene            complement(1021..1338)
                     /locus_tag="VGT87_001125"
     CDS             complement(1021..1338)
                     /locus_tag="VGT87_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEQ3813985.1"
                     /translation="MVLSPKPFEMIASGTKTIELRLNDEKRQKVQVDDAIVFVRTDDS
                     SQMLWTKVVELHYFDDFVQLYQSLPLDNCGYRKEELEMARPEDMERYYSQEQQAQYGG
                     SRH"
     gene            complement(1352..1822)
                     /locus_tag="VGT87_001126"
     CDS             complement(1352..1822)
                     /locus_tag="VGT87_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEQ3813986.1"
                     /translation="MAKYGFLSVLEEEMDKHFQYDYAMDWDKKNHAVEVTFVLEAQNK
                     EAIKTIDDSGEMTQDDIVFEDYVLFYNPAKSQFDAADYLVTIPFDAKKGFSREFLAYF
                     AQFLNDVAIEGHSDLMDFLADDSKADFFLEWNAQAFEEGQQGLEEGESYPYPRY"
     gene            1995..3968
                     /locus_tag="VGT87_001127"
                     /pseudo
     CDS             1995..3968
                     /locus_tag="VGT87_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061613.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
     gene            4153..5910
                     /gene="pabB"
                     /locus_tag="VGT87_001128"
     CDS             4153..5910
                     /gene="pabB"
                     /locus_tag="VGT87_001128"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEQ3813987.1"
                     /translation="MHRKTIIDFKELGQRYLFDEPLVELVAKTLDQVGPVIEKVQHYQ
                     QLGYYVVGYLSYEAAAFFDNALQTHNDRLGNEYLAYFTVHKTCQKKDLPLDYDSITIP
                     NQWVSATQKEAYQKAIETIHHEMRQGNTYQVNYTIQLTQELNAADSFAIYNKLVVEQA
                     AGYNAYIAHDEFAVISASPELFFKQEGNQLTTRPMKGTTKRGVNTWLDQQEHDWLQAD
                     GKNRSENMMIVDLLRNDMGKICQTGSVCVDRLCEVEQYSTVWQMTSTIVGDLKADCDL
                     IDILTALFPCGSITGAPKVSTMAIITSLEPNPRGIYCGTVGICLPDGRRFFNVPIRTI
                     QLSHNQATYGVGGGITWQSKWEDEYEEVHQKTAFLYRHKQIFDLKTTAKVEHQKIAFL
                     EQHLNRLKEAATYFAYPYDEKALQKQLSTYLENKNNAAYRLMIRLSKDGKISLSDQPL
                     EPLSADFLTAQLSLQKKDVTASPFTYFKTSYRPHIEQKSYEQLFYNQAGQLLETSIGN
                     LFVQLGQTLYTPPVAVGILPGLFRQELLATGQAQEKEVTLADLKEASAIFGGNAVRGL
                     YPLNLELTHLDALLAKSQA"
     gene            5943..6509
                     /locus_tag="VGT87_001129"
     CDS             5943..6509
                     /locus_tag="VGT87_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEQ3813988.1"
                     /translation="MILLIDNYDSFTYNLAQYLSEFDETIVLYNQDPNLYDMAKKADA
                     LVLSPGPGWPKEANQMPKLIQDFYQTKPILGVCLGHQAIAETLGGTLRLAKRVMHGRQ
                     STIETQDPASLFRSLPQEITVMRYHSIVVDQLPKGFSVTARDCDDQEIMAFEHHTLPL
                     FGLQFHPESIGTPDGMTMIANFIAAIPR"
     gene            6542..7810
                     /locus_tag="VGT87_001130"
     CDS             6542..7810
                     /locus_tag="VGT87_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEQ3813989.1"
                     /translation="MPDHLALRMRPKTISEVIGQKHLVGEGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEIAEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLENGTIIMIGATTENPFFSVTPAIRSRVQIFELEPLSNEDIKTAIQ
                     LAISDKERGFPFLVTIDDEALDFIVTATNGDLRSAYNSLDLAVMSTSPNEDSSRHISL
                     ETMENSLQRSYITMDKNGDGHYDVLSALQKSIRGSDVNASLHYAARLVEAGDLPSLAR
                     RLTIIAYEDIGLANPDAQVHTVTALDAAQRIGFPEARILIANVVIDLALSPKSNSAYL
                     AMDAALADLRTSGNLPIPRHLRDGHYAGSKDLGNAKDYLYPHAYPEKWVKQQYLPDKL
                     VGHHYFEANETGKYERALGSNKERIDKLLD"
     gene            7899..8095
                     /gene="ssrS"
                     /locus_tag="VGT87_001131"
     ncRNA           7899..8095
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VGT87_001131"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            8096..8168
                     /locus_tag="VGT87_001132"
     tRNA            8096..8168
                     /locus_tag="VGT87_001132"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8129..8131,aa:Lys,seq:ctt)
     gene            8383..9084
                     /locus_tag="VGT87_001133"
     CDS             8383..9084
                     /locus_tag="VGT87_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HEQ3813990.1"
                     /translation="MKKTKLIFSLTSIFIAIISRPVFGLEVDNNSLLRNIYSTIVYEY
                     SDTVIDFKTSHNLVTKKLDVRDARDFFINSEMDEYAANDFKDGDKIAVFSVPFDWNYL
                     SEGKVTAYTYGGVTPYQKTSIPKNIPVNLWINGKQISVPYNEISTNKTTVTAQEIDLK
                     VRKFLIAQHQLYSSGSSYKSGKLVFHTNDNSDKYSFDLFYTGYRDKESIFKVYKDNKS
                     FNIDKIGHLDIEIDS"
     gene            complement(9410..9634)
                     /locus_tag="VGT87_001134"
     CDS             complement(9410..9634)
                     /locus_tag="VGT87_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3813991.1"
                     /translation="MTYFRLKKELNWLLRSKGIVVYEGNQKLQIAINTGKNNSEKVFF
                     DTFFEVVRAAATFSTAKNICFGLGLLRSQK"
     gene            9813..11441
                     /locus_tag="VGT87_001135"
     CDS             9813..11441
                     /locus_tag="VGT87_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEQ3813992.1"
                     /translation="MSKYLKYFSIITLFLTGLILVACQQQKPQTKERQRKQRPKDEIV
                     VSMGAKLPHEFDPKDRYGVHNEGNITHSTLLKRSPELDIKGELAKTYHLSEDGLTWSF
                     DLHDDFKFSNGEPVTADDVKFTYDMLKADGKAWDLTFIKNVEVVGKNQVNIHLTEAHS
                     TFTAQLTEIPIVPKKHYNDKYKSNPIGSGPYMVKEYKAGEQAIFVRNPYWHGKKPYFK
                     KWTWVLLDENTALAALESGDVDMIYATPELASKKVKGTRLLDIPSNDVRGLSLPYVKK
                     GVITDSPDGYPVGNDVTSDPAIRKALTIGLNRQKVLDTVLNGYGKPAYSIIDKTPFWN
                     PKTAIKDNKVAKAKQLLTKAGWKEQADGSRKKGDLDAAFDLYYPTNDQLRANLAVEVA
                     EQAKALGITIKLKASNWDEMATKSHDSALLYAGGRHHAQQFYESHHPSLAGKGWTNIT
                     FYNNPTVTKYLDKAMTSSDLDKANEYWKLAQWDGKTGASTLGDLPNVWLVSLNHTYIG
                     DKRINVGKQGVHSHGHDWSLLTNIAEWTWDESTK"
     gene            11554..12531
                     /locus_tag="VGT87_001136"
     CDS             11554..12531
                     /locus_tag="VGT87_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ3813993.1"
                     /translation="MKRTTIIIIWKIIRCVTLIFGVSVLTFVLLKQSPVDPVMASVNY
                     DTSLTPAQYKAIAHHYGLDKPALVQYFIWLKNVIQGHLGTSLVYRQPVSDIIKSRAGA
                     SFILMGLSWILSGLIGFILGTLSAFHQGKLLDRVVRWFSYLQISVPTFWIGLIFLLIF
                     SVQLGWFPIGISSPIGTLSQDITLADRIKHLMLPVFTLSILGIANVTLHTRTKMMSVL
                     SSEYVLFARARGETQWQIFKHHCLRNAIVPAITLHFSYFGELFGGSVLAEQVFSYPGL
                     GSTLTEAGLKSDTPLLLAIVMIGTLFVFAGNLIADILNSIINPQLRRKV"
     gene            12528..13349
                     /locus_tag="VGT87_001137"
     CDS             12528..13349
                     /locus_tag="VGT87_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ3813994.1"
                     /translation="MILKRRTMVLWQLGIAISLILSILALNLYFYRTPLETNAALRNL
                     APSLNHLFGTDGLGRDMFVRTIKGLYFSLQVGLLGALMGVFLATVFGVLAGLGNSLID
                     KIIAWLVDLFIGMPHLIFMILISFVVGKGAQGVIIATAVTHWPSLARLIRNEVYDLKN
                     KAFVQLSKSMGKTPYYIVRHHILPLIASQIFIGFILLFPHVILHEASMTFLGFGLSAE
                     QPSVGIILSEAAKHISLGNWWLVIFPGLYLILVVNAFDTIGESLKKLFYPQTDHF"
     gene            13361..>14153
                     /locus_tag="VGT87_001138"
     CDS             13361..>14153
                     /locus_tag="VGT87_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ3813995.1"
                     /translation="MTETLLSIKDLSITFTQYGRFLKPFQSTPIQALNLEVKKGELLA
                     IIGASGSGKSLLAHAIMDILPKNAAVTGDMIYRGQSLTSKRIRQLRGKEMTLIPQSVN
                     YLDPSMKVKHQVRLGISENAKATQEGLFQQFGLKESDGDLYPFQLSGGMLRRVLFTTC
                     ISDTVSLIIADEPTPGLHPDALQMVLDQLRSFADKGISVIFITHDIVAASQIADRITI
                     FKEGKAIETAPASFFSRGGEQLQTEFARRLWRTLPQQDFLKGVTHD"
BASE COUNT         4307 a         3060 c         2640 g         4146 t
ORIGIN      
        1 tttttttgta tcaataacag ctgaaaatat tgacaaaatt atcaataaaa atactaacaa
       61 taactcaata ggtagagaaa atgtataggt attactaata aacaataacg ccgtattgaa
      121 cattaataat tctctccact gatttcttat tatttctaca acagaggatt cttttaataa
      181 taagatagct ggaaaaatcg taaataaaaa aataataaca taatctttta tataccaata
      241 agataactca atatcattct ttgtcacgta atatattaaa acaatataga aaatattagc
      301 tacacttacc cactgagata taggaagctt taacactatc cagatactaa tcaatatgtc
      361 taaaacactt ttaaaaattc ctttttttat gcaaaaataa aataccagaa tcaataaaac
      421 tattatccat attgtaactg caatttctct agtagtcata tacctccatc taatctctca
      481 atcgtccttc caaactccca tagacttggc catttctcac aatccttaaa cactgaaata
      541 ccaacactca tctcactggt cacttttctc agctagataa gctgccaatc gttcttgtaa
      601 ttgaggcaca gccttcttat ccgctaaaaa atccttcacc ggtacaaact tgtaagcctg
      661 accttcttct ccaaaatgaa tggctttaac aagatcctta ctaatcatgg ccaccatgaa
      721 aatagagtaa gtctccggat tcataacccc ttgataagct ttttcccaga cgaccatatc
      781 ttgcgaaatc gcaattccta gttcttccat cacttcacgt tgcacacatt caaagggtgt
      841 ttctaaacct tctcggcctc caccaggcaa atcccaggtg tcaggaaaag gaatggtaga
      901 cttattatct cttaaaatgg tcacaaggtg ttggtcttgc attaaagcaa ttttcacccc
      961 agaaaaaacg catgcctgtg tcgatgatgt cataatcgtc tcctctacag cactgataac
     1021 tcaatgccga ctacccccat actgggcttg ctgttcctga ctataatagc gttccatgtc
     1081 ttctggacga gccatctcca gttcttcttt ccgatagcca cagttatcta agggtaacga
     1141 ctggtaaagc tgaacaaagt cgtcaaaata atgaagctca accacctttg tccacaacat
     1201 ctgactacta tcatctgttc taacaaatac gatggcatca tcaacttgga ccttttgccg
     1261 cttctcgtca ttcagacgca attcaatcgt ctttgtacct gaggcaatca tctcaaaagg
     1321 tttaggactt agcaccatct cgtgtgtcac cttagtacct tgggtaaggg taactttctc
     1381 cctcttctaa tccttgttga ccttcttcaa aagcttgcgc gttccactcc aagaagaaat
     1441 cagccttgct gtcatccgct aagaaatcca ttaaatcgct gtgcccttca atagcaacat
     1501 cgttgagaaa ttgcgcaaag taagctaaga attcacgaga aaagcccttt ttagcgtcaa
     1561 aaggaatagt aaccaaataa tctgctgcat caaattgaga cttggcaggg ttgtaaaaga
     1621 gcacatagtc ctcaaagaca atatcatctt gggtcatttc gccactatca tcaatggttt
     1681 ttatggcctc tttattttga gcttctaaaa caaaagtcac ttcaaccgca tgatttttct
     1741 tgtcccaatc catagcataa tcatactgaa aatgcttatc catttcttct tctaatactg
     1801 ataaaaagcc atattttgcc atctaattgt cctttttcct aacgattatt tgcttccatt
     1861 gtagcataat ttgaactaac tttcactggc aaatgctgac tgatgggcct tctgtatttt
     1921 agtaagcgtt ttacccctta ctttcctccc attatgttaa actaataagc gattatgatg
     1981 ttaggagaaa aagaatgaga tattctagac gctacccttc tcagacaaga cagatgcttc
     2041 ttgccttgtt attaatgact gccagcctac aagctggagc tatgactact tatgcggatg
     2101 aggttaatac caaccatcaa ccactaacgg cccaagtgga agctagtaag ccgacagcca
     2161 tggaatctgt cacttcacct gcagaccaga cccatccaat ctcaacgcaa gaggcttcgt
     2221 ctcctttaca ccctttaaca acagaggcca ctcctgccgc ccaagaatct ccgattacct
     2281 tagaagacta taaggtagcc agtgcgagcc aattagctga gtgggctcgc cagcaacggg
     2341 tcacaggcca acagctgcta gattttgcct tggaaaccat caaagaaact aaccctgagc
     2401 tcaataatgt catttctctg cgtgaaccat tggctcgaca agagagcgag caaatgacag
     2461 atgaggggca acccttttac aaggttccta ttctggttaa agggttagga cacaccgttg
     2521 ctggaagtag caacaccaac gggcttgcct ttttgaaaga caaaaccagc tcatctacca
     2581 gcgcctttgt caaacaactc caaaaggctg gttttatcgt ggtagggcag tcctctttcc
     2641 ctgaaatggg atggattaat gtgacgaatt ccaatcttta cggaaataca cataacccat
     2701 ggcagcttga ccaaaatcct ggcgggtcct ctggtggctc tgcagctgct gtggcttcag
     2761 gacaagtcag tctagccagt gcttctgatg gtgggggctc aacccgcatt ccagcttctt
     2821 ggagtggctt gattggccta cacccaacac gtggcatttt agagggaaat ccgacttctg
     2881 aacgtagtaa tgttagtcat tttgctctaa ctaagagcat ggaagacact gaaaagctct
     2941 tccaattcct cttaaaagac aaggctaaag cgcaacaaaa cccacagcgt ttagacacaa
     3001 gtatccccat tgcttacagc acccagacac cagcttgaac accaattagt gaggaagcta
     3061 ttgctgcgat caaagaagct gtagcctttt tacagaagca aggttatcaa accgtagaag
     3121 tgccttaccc cgttgatggt aagctcatga tgcaatacta ttacactatt gctgcttctg
     3181 cagcgccatc tatcaatttt atggcacaac aaaccttgaa acgccccctt caaaaagagg
     3241 atgtcgaact cctatcatgg gcgctctacc aaactgggaa agacttgacc aaagaggatg
     3301 tcaacaaagc ctgggagggt atcgcagcca tgaccgagca attaaaccag ttctaccaaa
     3361 aataccccat cttcttaaca ccgacaacag cctatcccgc ccctgctgct gattatcacc
     3421 acattcctaa agatctagta gcacagctca gtgacatgtc aggcttgagc aaagaagaaa
     3481 aattggacct tatttaccgg caatggttac ctgcatggac gctaacacca tttacccagc
     3541 ttgctaacct gacgggaaca ccatccttgt ctttaccaac ccatctgacg aaaagcggtc
     3601 tacctcttgg cattttggtg aatagcggag ctcataacga tagtctcctg ttacaactgg
     3661 gacagctctt tgaaaaagca aatcgtttcc atatcctgac ggctggcaaa aaaggattgc
     3721 cagaaactcc tgcgcatgag cataacctct caactcaatc tgaaaacaaa caaggcgttg
     3781 ccattccagt gacttatcaa accaagggat ttactactgg accaaccaag ggccagaacg
     3841 gcttagtaac cctccctcaa actggtgatg gtcagtcaaa aggtgtgttg ttaactagct
     3901 acatcagcct tttccttgga accctcttct tagcaggaag cctctggagc aataaggtaa
     3961 aagactaggc tattgtcgtc ggagatttaa cctacctaaa cattcactgc tgcttgcctt
     4021 acgataagag tatattgtag tcaatcaaga ctgaactttc ttagtgaaag ttcagtcttt
     4081 ttacgtggcc aaaagacagt tgttgcctgt tctcattagg cttagcaacc gcttttatga
     4141 tataatttgg gtatgcatag aaaaacgatt attgatttca aagagctcgg gcaacgctat
     4201 ctttttgatg agccattagt agaattggta gccaaaacct tagatcaggt cggcccagtc
     4261 attgaaaaag tacaacacta ccaacagctg ggctattacg tggtgggcta tctcagttat
     4321 gaagcagctg ctttttttga caatgcctta caaacccata atgacagact aggcaacgag
     4381 taccttgcct attttacagt gcacaaaacc tgtcaaaaaa aagacctacc tcttgattat
     4441 gacagcatta ctatccctaa ccaatgggtc agcgccactc aaaaagaagc ctatcaaaaa
     4501 gccattgaaa ccattcacca tgagatgcga cagggcaata cttatcaggt taactatacc
     4561 attcaattaa ctcaggaact caatgcagct gacagcttcg ccatttacaa taaactcgtc
     4621 gttgaacagg ctgctggcta caatgcctat atcgcccatg atgagtttgc cgttatttca
     4681 gctagtcctg agttgttctt taaacaggaa gggaatcaac tcaccaccag acctatgaag
     4741 ggcaccacca agcgcggggt taacacctgg ctggatcaac aagaacatga ttggctccaa
     4801 gctgacggga aaaatcgctc tgaaaacatg atgattgttg acttgctccg caatgacatg
     4861 ggaaaaattt gccaaacagg cagtgtttgt gtcgataggc tctgtgaggt ggaacaatac
     4921 tctaccgtct ggcaaatgac ctcaaccatt gttggtgacc tcaaagcgga ctgtgacctg
     4981 atagacattc tcacagctct ctttccttgt ggctctatca caggggctcc taaagtctcc
     5041 accatggcga ttatcacatc tctggagcca aacccaaggg ggatttattg tggaactgtt
     5101 ggcatttgtc tacctgacgg tcgccgtttc ttcaatgttc ccattagaac catccagctc
     5161 agtcataatc aagctactta tggtgtcgga ggcggtatca cttggcaaag caagtgggaa
     5221 gacgaatacg aagaagtcca tcaaaaaaca gcttttctct atcggcacaa acaaatcttt
     5281 gacctcaaaa caaccgccaa ggtggaacac caaaaaatag ccttccttga gcaacacctc
     5341 aatcgcctta aagaagcagc cacttatttt gcttaccctt acgatgagaa agctttgcag
     5401 aaacaactgt caacctattt ggaaaataag aataatgctg cctaccgttt gatgattcgt
     5461 ttatccaaag atggaaagat tagcctctct gatcaaccct tggaaccttt gtcagccgat
     5521 tttctgacag ctcaactctc tttacagaaa aaggacgtga cagcttcgcc ttttacctac
     5581 tttaagacca gctaccggcc tcatattgag caaaaatctt atgaacagct attctataac
     5641 caagcggggc aattattaga aacgtccatt ggcaatctct ttgttcagct gggtcagacc
     5701 ctctacacac caccagtggc tgtggggatt ttaccaggac tttttcgcca agagctgcta
     5761 gctactggtc aagctcagga aaaagaggtg acactagctg atttaaaaga ggctagcgct
     5821 atttttggag gaaatgctgt ccgtggcctc tatcctctca accttgagct tactcacctt
     5881 gatgccctct tagctaagag tcaagcctaa actgccactc atcttttgtt attaaggaac
     5941 ctatgatact cttaattgat aattacgatt catttaccta caacctcgcc caatatttaa
     6001 gtgaatttga cgagacgatt gtcttgtata accaagaccc aaacttatat gacatggcca
     6061 aaaaggccga cgcgctagtc ctctcacctg gtcctggttg gcccaaggaa gccaaccaaa
     6121 tgccaaaact cattcaagac ttttaccaaa caaaacctat cttaggagtg tgtctgggac
     6181 accaagcgat cgctgaaaca ttagggggaa ccttacgctt ggccaaacgc gtcatgcacg
     6241 ggagacaaag caccattgaa acgcaagacc ctgctagtct ttttcgctcc ctgccacaag
     6301 agatcaccgt catgcgctac cattccatcg ttgtggatca gttaccaaaa ggttttagcg
     6361 taactgctag agactgtgac gatcaagaaa tcatggcatt tgaacaccac accctgccac
     6421 tttttgggct acaatttcac ccagaaagca tcggaactcc tgatggcatg accatgattg
     6481 ctaacttcat cgcagctatt ccccgttaat cccatccccc aaaagaaagg aggacagtta
     6541 catgcctgat catttagcgc tcagaatgcg ccccaaaacc atttcagagg tcatcggaca
     6601 aaaacacctt gttggcgaag gcaaaatcat ccgtcgcatg gtggaagcaa atagactatc
     6661 gtccatgatc ctctatggcc ctccaggaat cggtaaaact tccattgctt ctgctattgc
     6721 aggtactacc aagtacgcct ttcggacctt taatgccacc gttgatagca aaaaacgtct
     6781 ccaagaaatt gcggaagaag ctaaattttc tggtggcttg gtattattac tagacgagat
     6841 tcatcgcctt gacaagacaa aacaagattt tcttttacct ttacttgaga atggcaccat
     6901 tatcatgatt ggcgcaacca ctgagaaccc cttcttttca gtgacacccg ctatcagaag
     6961 cagggtccag atttttgaat tggaaccact ttctaacgag gatattaaaa cagccattca
     7021 gctagcaatt tccgacaaag aacggggatt tcctttttta gtgacaattg atgatgaggc
     7081 gctagatttc attgttaccg ccacaaatgg agacctacga tctgcttata attccttgga
     7141 tctagcagtc atgtctactt ctcctaatga agatagtagc cgtcatatta gtctagagac
     7201 aatggaaaat agtctgcagc gtagttatat taccatggat aaaaacgggg acgggcatta
     7261 tgatgtttta tctgccttac aaaaatccat tagaggttct gatgtcaatg ccagcctgca
     7321 ctacgccgct cgtttagtag aagctggaga cttgcctagt ttagcaagac gattgaccat
     7381 tattgcttac gaagatattg gcttagccaa ccctgacgcc caagttcata ccgtcaccgc
     7441 actagatgcc gctcaacgca ttggttttcc agaagctcgt attcttatcg ctaacgtggt
     7501 cattgatttg gctctatcac ctaaatccaa ttcagcttat ttagctatgg atgcagcttt
     7561 agctgatttg agaacatcag gtaacctccc cattccaagg catcttagag acgggcatta
     7621 cgctggcagc aaggacctag gcaatgccaa agattacctc tacccacacg cctaccctga
     7681 aaaatgggtc aaacaacaat acctaccaga taaattagta ggacatcatt attttgaagc
     7741 caatgaaacc ggtaaatacg aacgcgcact tggttcaaat aaagagcgta ttgataagct
     7801 cttagactga ctgctagttt taaaaatcgc aaccgatttc aaaaatcgat aattttgctt
     7861 gaaaaaattt taaaaagtgg tattataaaa gtacagaaac attgctgtgg catacgaatc
     7921 cacatctaag tgttgaccga ctatttcttt gtattttcgg gaaacaaaga ctctttctag
     7981 tctgcaagcc gtttcacgcg gacgcaactt ccaagaaaga gcgcgttacc cacctgctta
     8041 tactgcgcgg gctcaataca aaaacgtgga tcaccggtgc caatacagca ttcttgcctc
     8101 cttagctcag ttggtagagc agtagactct taatctatgg gtcacaggtt cgagccctgt
     8161 agggggtata caactataca taaaaaagcc tttgtttaaa ggcttttttg attgtttcat
     8221 aactaatcgg cttaattaaa agcgtcaatc cacttttagt atcatgtctc aattttcatt
     8281 tgatagtcag caagtatatc tacaaaattg ttaatgatta tttcttttga aagtgttata
     8341 ttaaaacata ataaaataat aactcctgaa aagaggctat ttatgaaaaa aacaaaactt
     8401 attttttctt tgacttcaat attcattgca ataatttctc gtcctgtgtt tggattagaa
     8461 gtagataata attcccttct aaggaatatc tatagtacga ttgtatatga atattcagat
     8521 acagtaattg attttaaaac cagtcataac ttagtgacta agaaacttga tgttagagat
     8581 gctagagatt tttttattaa ctccgaaatg gacgaatatg cagccaatga ttttaaagat
     8641 ggagataaaa tagctgtgtt ctccgtccca tttgattgga actacttgtc agaaggaaaa
     8701 gtcacagcat atacgtacgg cggagtaaca ccctaccaaa aaacttcaat acctaaaaat
     8761 atccctgtta atttatggat taatggaaag cagatctctg ttccttacaa cgaaatatca
     8821 actaacaaaa caacagttac agctcaagaa attgatctaa aggttagaaa atttttaata
     8881 gcacaacatc aattatattc ttctggttct agctacaaaa gtggtaaatt agtttttcat
     8941 acaaatgata attcagataa atattctttc gatcttttct atacaggata tagagataaa
     9001 gaaagtattt ttaaagtata caaagacaat aaatctttca atatagataa aattgggcat
     9061 ttagatatag aaattgactc ctaattcaat ttttcaatat aacttttata cactaagcta
     9121 ttccaaatag attgaatcct ctaaaaagta agtatgtctt tttttatatc acttaggttt
     9181 tgttctaaat ttgttctaga atgctatact ttctatatta attttaaaaa acaaaaattg
     9241 ataaattcct tgataatact agccattttt gtttataaat tttctttatt atgctgtttc
     9301 tacttgtagg gggtatgata caaaaatgac aaaagctttt agaacgtaga cagacccagt
     9361 gtgatgctca aaaagcatga actactctgc taatgtgata cccgatttcc tatttctgcg
     9421 aacgaagtaa gccaagacca aaacagatgt tttttgctgt ggagaaagtc gctgccgccc
     9481 gaaccacctc aaaaaaagta tcaaaaaaga ctttttcact gttattttta cctgtattaa
     9541 tagctatctg aagtttttga ttcccttcgt aaactacaat tcctttactt cttagtaacc
     9601 agtttaattc tttttttaat ctaaaataag tcatgaaacc tctttatctt ttgttaatac
     9661 atcaacttat aaaaaagccc tgagtatctt cctgaaaata acaaaaattc taattaacca
     9721 gttaaacaat tgccctccta tttgtttatt gctataatga gatggagtcg ttactattat
     9781 tatttattaa ttagaaataa ggagatttga ttgtgtcaaa atacctaaaa tacttctcta
     9841 ttatcacgtt atttttgact gggcttattt tagttgcatg tcaacaacaa aagcctcaaa
     9901 caaaagaacg tcagcgcaaa caacgtccaa aagacgaaat tgtcgtttct atgggggcaa
     9961 agctccctca tgaattcgat ccaaaggacc gttatggagt ccacaatgaa gggaatatca
    10021 ctcatagcac tctattgaaa cgttctcctg aactagatat aaaaggagag cttgctaaaa
    10081 cataccatct ctctgaagat gggctgactt ggtcgtttga cttgcatgat gattttaaat
    10141 tctcaaatgg tgagcctgtt actgctgatg atgttaagtt tacttatgat atgttgaaag
    10201 cagatggaaa ggcttgggat ctaaccttca tcaagaacgt tgaagtagtt gggaaaaatc
    10261 aggtcaatat ccatttgact gaggcgcatt cgacatttac agcacagttg actgaaatcc
    10321 caatcgtccc taaaaaacat tacaatgata agtataagag caatcctatc ggttcaggac
    10381 cttacatggt aaaagaatat aaggctggag aacaagctat ttttgttcgt aacccttatt
    10441 ggcatgggaa aaaaccatac tttaaaaagt ggacttgggt cttacttgat gaaaacacag
    10501 cactagctgc tttagaatct ggtgatgttg atatgatcta cgcgacacca gaacttgcta
    10561 gtaagaaagt caaaggcact cgcctccttg acattccatc aaatgatgtg cgcggcttat
    10621 cattacctta tgtgaaaaaa ggcgtcatca ctgattctcc tgatggttat cctgtaggaa
    10681 atgatgtcac tagtgatcca gcaatccgca aagccttgac tattggttta aatagacaaa
    10741 aagttctcga tacggtttta aatggttatg gtaaaccagc ttattcaatt attgataaaa
    10801 caccattttg gaatccaaaa acagccatta aagataataa agtagctaaa gctaagcaat
    10861 tgttgacaaa agcgggatgg aaagaacaag cagacggtag ccgtaaaaaa ggtgaccttg
    10921 atgcagcgtt tgatctgtac taccctacta atgatcaatt gcgagcgaac ttagccgttg
    10981 aagtagcaga gcaagccaaa gccctaggga ttactattaa actcaaagct agtaactggg
    11041 atgaaatggc aacgaagtca catgactcag ccttacttta tgccggagga cgtcatcacg
    11101 cgcagcaatt ttatgaatca catcatccaa gcctagcagg taaaggttgg accaatatta
    11161 cgttttataa caatcctacc gtgactaagt accttgacaa agcaatgaca tcttctgacc
    11221 ttgacaaagc taacgaatat tggaagttag cgcagtggga tggcaaaaca ggtgcttcta
    11281 ctcttggaga tttgccaaat gtatggttgg tgagccttaa ccatacttat attggtgata
    11341 aacgtatcaa tgtaggtaaa caaggcgtcc acagtcatgg tcatgattgg tcattattga
    11401 ctaacattgc cgagtggact tgggatgaat caactaagta acgcgtttag ttaatacagc
    11461 gactttaagt ttggagtctg agaaaacaag atacacaaaa ctgtgctgat cgtttcctcg
    11521 gctccttttc ttatcgatta aggagatttc attgtgaaac gtactaccat tattatcatc
    11581 tggaaaatca tcagatgtgt cacgcttatt tttggggtat ctgttttgac cttcgttttg
    11641 ttaaaacaat ctccagtaga tccagtcatg gcaagtgtca attatgacac atcactaacc
    11701 cctgctcagt acaaagcgat tgctcaccac tatggcttgg ataagccagc tctagtccaa
    11761 tattttattt ggttaaaaaa tgtgatacag ggacatttag ggacctcgct cgtttatcgg
    11821 caacctgtta gtgatattat taaatcacgg gcaggtgctt ctttcatact tatgggactc
    11881 tcttggatct tatcgggtct tattggattt atcttaggaa cgttatccgc tttccatcaa
    11941 gggaaattac ttgatcgagt tgtcaggtgg ttttcttacc ttcagatatc agtaccaacg
    12001 ttttggattg gactcatttt tttattaatc ttttctgtcc agctggggtg gttcccgatt
    12061 ggtatttctt ctccgatagg cactttaagt caagatatta cgttagctga tcggattaag
    12121 caccttatgt tacctgtttt cacgctaagt attctaggca ttgccaatgt cactcttcat
    12181 acgagaacta aaatgatgtc ggtgctttcc agtgaatatg tcttatttgc cagagcgcgt
    12241 ggggaaacac agtggcaaat ttttaaacat cattgtctta gaaatgctat cgtaccagct
    12301 attacactgc atttttccta ttttggagaa ttgtttggag gatctgttct tgctgagcaa
    12361 gttttctcat atcctggctt agggtctacc ctcactgaag caggccttaa aagtgataca
    12421 ccgctccttc tagctattgt gatgataggg acattatttg tttttgcggg caatcttatt
    12481 gcggacattt taaatagcat catcaatcca cagttaagga gaaaagtatg atattgaaac
    12541 gtcgaacgat ggttttatgg caactaggta tcgccatttc tctcattctt agtattctag
    12601 ccttaaacct ttatttctat aggacgcctt tggaaaccaa tgcagcttta cgcaacctcg
    12661 ctccttcctt aaaccatctt tttggaacag atggtttagg tagggatatg tttgtcagaa
    12721 cgattaaagg gctttatttc tctttacaag tcggcttatt aggtgccctt atgggagtct
    12781 ttcttgcgac cgtttttgga gtgcttgcag gtctaggaaa tagccttatt gataaaataa
    12841 tagcctggtt ggttgatttg tttattggta tgcctcattt gatttttatg attctcattt
    12901 cttttgttgt tgggaaaggg gctcaagggg ttatcattgc aacggctgtt acccattggc
    12961 cctctctagc aaggcttatc cgcaatgaag tctatgatct aaagaataaa gcctttgtcc
    13021 agctctctaa aagcatggga aaaacgcctt attatattgt gaggcatcat atcctgcctt
    13081 tgattgcttc tcaaattttc attgggttta tcctcttatt tccgcacgtc atcttgcatg
    13141 aagcatccat gactttctta ggatttggcc tttctgccga acaaccttcg gttggtatca
    13201 ttctgtcaga ggcagctaag catatctctc ttggcaattg gtggttggtg atttttccag
    13261 gcctttatct tattttggtt gtcaatgcct ttgatactat cggagaatct ttaaagaaac
    13321 tcttttaccc tcaaacggat catttttagg aggtagagat atgacagaaa cattattaag
    13381 cattaaagac ctctccatca ccttcactca atacggaaga tttttaaaac catttcaatc
    13441 cacaccgata caagcgctga atttagaagt taaaaaaggt gagttattag ctattatagg
    13501 tgctagtggt tcaggcaaga gtttattagc acatgctatt atggatattc ttcctaaaaa
    13561 tgcagctgta acaggagata tgatttatcg tggtcaatca ctaacttcta aacgcatcag
    13621 acagttgcga ggaaaagaaa tgacgttgat tccacaatcc gttaattatt tagatccatc
    13681 tatgaaagtc aaacatcagg tgcgcttggg tatctcagaa aatgctaagg ctactcaaga
    13741 aggattgttt caacagtttg gattaaaaga aagtgatggt gacttgtatc ctttccaact
    13801 ttctggcgga atgctccgac gtgttttgtt tacaacgtgt attagtgata cggtttcttt
    13861 gattattgcg gatgagccca cccctggatt acatccagat gctctgcaaa tggttttaga
    13921 tcaactacgc tcttttgcag ataaaggaat aagcgtcatc tttatcactc atgatattgt
    13981 agcagctagt caaattgcgg atcgtataac tatttttaaa gagggaaaag ctattgaaac
    14041 agccccagct agctttttta gcagaggtgg agagcaatta caaacagaat ttgcgagacg
    14101 tttatggcgc acactccctc agcaagactt tttgaaagga gttactcatg acc
//
LOCUS       DAUCLR010000046        13943 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.046, whole genome shotgun sequence.
ACCESSION   DAUCLR010000046 DAUCLR010000000
VERSION     DAUCLR010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13943
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.046"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(151..1069)
                     /locus_tag="VGT87_001139"
                     /pseudo
     CDS             complement(151..1069)
                     /locus_tag="VGT87_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            1255..1328
                     /locus_tag="VGT87_001140"
     tRNA            1255..1328
                     /locus_tag="VGT87_001140"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1289..1291,aa:Arg,seq:tct)
     gene            complement(1485..1964)
                     /gene="rlmH"
                     /locus_tag="VGT87_001141"
     CDS             complement(1485..1964)
                     /gene="rlmH"
                     /locus_tag="VGT87_001141"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEQ3813996.1"
                     /translation="MKVKLICVGKLKERYLKDGISEYQKRLSRFCQFEMIELTDERTP
                     DKASFADNQLIMSKEAQRIHKKIGERDFVIALAIEGKQFPSETFSELISGVTVKGYST
                     ITFIIGGSLGLDSIIKKRANMLMSFGLLTLPHQLMRLVLTEQIYRAFMITQGSPYHK"
     gene            2176..3399
                     /locus_tag="VGT87_001142"
     CDS             2176..3399
                     /locus_tag="VGT87_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEQ3813997.1"
                     /translation="MPSMKHILKSLSILLVGFLGGLIAIITFNNLYPHSPSKINSGKA
                     TTSNMVFNNTTNTTKAVKAVQNAVVSVINYQDNPSSSLSNPYTKLFGEGRSKENKDAE
                     LSIFSEGSGVIYRKDGNSAYVVTNNHVIDGAKRIEILMADGSKVVGELVGADTYSDLA
                     VVRISSDKIKTVAEFADSTKLNVGEVAIAIGSPLGTQYANSVTQGIVSSLSRTVTLKN
                     ENGETVSTNAIQTDAAINPGNSGGPLINIEGQVIGINSSKISSTPTGSNGNSGAVEGI
                     GFAIPSTDVIKIIKQLETNGEVIRPALGISMVNLNDLSTNALSQINIPTSVTGGIVVA
                     EVKEGMPASGKLAQYDVITEIDGKIVNSISDLQSSLYGHDINDTIKVTFYRGTTKKKA
                     DIKLTKTTQDLTKTQ"
     gene            3458..4264
                     /locus_tag="VGT87_001143"
     CDS             3458..4264
                     /locus_tag="VGT87_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEQ3813998.1"
                     /translation="MFLILSRNSLMTKELLIDLPIEDIVTNPYQPRIQFNQRELQDLA
                     TSIKSNGLIQPIIVRKSDIFGYELVAGERRLKASKMAGLKKIPAIIKKISTLESMQQA
                     IVENLQRSNLNAIEEAKAYQLLVEKKHMTHDEIAKYMGKSRPYISNTLRLLQLPAPII
                     KAIEEGKISAGHARALLTLSDDKQQLYLTHKIQNEGLSVRQIEQLVTSTPSSKLSKKT
                     KNIFATSLEKQLAKSLGLSVNMKLTANHSGYLQISFSNDDELNRIINKLK"
     gene            4467..5822
                     /gene="dnaA"
                     /locus_tag="VGT87_001144"
     CDS             4467..5822
                     /gene="dnaA"
                     /locus_tag="VGT87_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEQ3813999.1"
                     /translation="MTENEQIFWNRVLELAQSQLKQATYEFFVHDARLLKVDKHIATI
                     YLDQMKELFWEKNLKDVILTAGFEVYNAQISVDYVFEEDLMIEQNQTKINQKPKQQAL
                     NSLPTVTSDLNSKYSFENFIQGDENRWAVAASIAVANTPGTTYNPLFIWGGPGLGKTH
                     LLNAIGNSVLLENPNARIKYITAENFINEFVIHIRLDTMDELKEKFRNLDLLLIDDIQ
                     SLAKKTLSGTQEEFFNTFNALHNNNKQIVLTSDRTPDHLNDLEDRLVTRFKWGLTVNI
                     TPPDFETRVAILTNKIQEYNFIFPQDTIEYLAGQFDSNVRDLEGALKDISLVANFKQI
                     DTITVDIAAEAIRARKQDGPKMTVIPIEEIQAQVGKFYGVTVKEIKATKRTQNIVLAR
                     QVAMFLAREMTDNSLPKIGKEFGGRDHSTVLHAYNKIKNMISQDESLRIEIETIKNKI
                     K"
     gene            5977..7113
                     /gene="dnaN"
                     /locus_tag="VGT87_001145"
     CDS             5977..7113
                     /gene="dnaN"
                     /locus_tag="VGT87_001145"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEQ3814000.1"
                     /translation="MIQFSINRTLFIHALNATKRAISTKNAIPILSSIKIEVTSTGVT
                     LTGSNGQISIENTIPVSNENAGLLITSPGAILLEASFFINIISSLPDISINVKEIEQH
                     QVVLTSGKSEITLKGKDVDQYPRLQEVSTENPLILKTKLLKSIIAETAFAASLQESRP
                     ILTGVHIVLSNHKDFKAVATDSHRMSQRLITLDNTSADFDVVIPSKSLREFSAVFTDD
                     IETVEVFFSPSQILFRSEHISFYTRLLEGNYPDTDRLLMTEFETEVVFNTQSLRHAME
                     RAFLISNATQNGTVKLEITQNHISAHVNSPEVGKVNEDLDIVSQSGSDLTISFNPTYL
                     IESLKAIKSETVKIHFLSPVRPFTLTPGDEEESFIQLITPVRTN"
     gene            7188..7385
                     /locus_tag="VGT87_001146"
     CDS             7188..7385
                     /locus_tag="VGT87_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEQ3814001.1"
                     /translation="MYQIGSFVEMKKPHACVIKETGKKANQWKVLRVGADIKIQCTNC
                     QHVIMMSRYDFERKLKKVLQP"
     gene            7715..8830
                     /gene="ychF"
                     /locus_tag="VGT87_001147"
     CDS             7715..8830
                     /gene="ychF"
                     /locus_tag="VGT87_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEQ3814002.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTELITPKKTVPTTFEFTDIAGIVKGASRGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPIADIDTINLELILADLESINKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTEDEAKVVKGLFLLTTKPVLYVANVDEDKV
                     ANPDGIDYVKQIRDFAATENAEVVVISARAEEEISELDDEDKEEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGIKAPQAAGIIHSDFERGFIRAVTMSYDD
                     LMTYGSEKAVKEAGRLREEGKEYVVQDGDIMEFRFNV"
     gene            8900..9469
                     /locus_tag="VGT87_001148"
     CDS             8900..9469
                     /locus_tag="VGT87_001148"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEQ3814003.1"
                     /translation="MVKMIVGLGNPGSKYEKTKHNIGFMAIDNIVKNLDVTFTDDKNF
                     KAQIGSTFINHEKVYFVKPTTFMNNSGIAVKALLTYYNIDITDLIVIYDDLDMEVSKL
                     RLRSKGSAGGHNGIKSIIAHIGTQEFNRIKVGIGRPLKGMTVINHVMGQFNTEDNIAI
                     SLTLDRVVNAVKFYLQENDFEKTMQKFNG"
     gene            9472..12975
                     /gene="mfd"
                     /locus_tag="VGT87_001149"
     CDS             9472..12975
                     /gene="mfd"
                     /locus_tag="VGT87_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEQ3814004.1"
                     /translation="MDILELFSQNKKVQSWHSGLTTLGRQLVMGLSGSSKALAIASAY
                     LDDQKKIVVVTSTQNEVEKLASDLSSLLDEELVFQFFADDVAAAEFIFASMDKALSRI
                     ETLQFLRNPKSQGVLIVSLSGLRTLLPNPDVFTKSQIQLTVGENYDSDTLTKQLMTIG
                     YQKVSQVISPGEFSRRGDILDIYEITQELPYRLEFFGDDIDSIRQFHPETQKSFEQLE
                     GIFINPASDLIFEASDFQRGIEQLEKALQTAQDDKKSYLEDVLAVSKNGFKHKDIRKF
                     QSLFYEKEWSLLDYIPKGTPIFFDDFQKLVDKNARFDLEIANLLTEDLQQGKALSNLN
                     YFTDNYRELRHYKPATFFSNFHKGLGNIKFDQMHQLTQYAMQEFFNQFPLLIDEIKRY
                     QKNQTTVIVQVESQYAYERLEKSFQDYQFRLPLVSANQIVSRESQIVIGAISSGFYFA
                     DEKLALITEREIYHKKIKRRARRSNISNAERLKDYNELAVGDYVVHNVHGIGRFLGIE
                     TIQIQGIHRDYVTIQYQNSDRISLPIDQIGSLSKYVSADGKEPKINKLNDGRFQKIKQ
                     KVARQVEDIADDLLKLYAERSQQKGFSFSPDDDLQRAFDDDFAFVETEDQLRSIKEIK
                     ADMESMQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVAVLVPTTVLAQQHYENFKA
                     RFENYPVEVDVLSRFRSKKEQAETLERVRKGQIDIIIGTHRLLSKDVVFSDLGLIVID
                     EEQRFGVKHKETLKELKTKVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPVQ
                     TYVLENNPGLVREAIIREMDRGGQIFYVYNKVDTIEKKVAELQELVPEASIGFVHGQM
                     SEIQLENTLIDFINGDYDILVATTIIETGVDISNVNTLFIENADHMGLSTLYQLRGRV
                     GRSNRIAYAYLMYRPDKVLTEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNILGA
                     SQSGFIDSVGFEMYSQLLEQAIASKQGKTTVRQKGNTEINLQIDAYLPDDYIADERQK
                     IDIYKRIREIRSREDYLNLQDELMDRFGEYPDQVAYLLEIALLKHYMDNAFAELVERK
                     NNQVIVRFEVTSLTYFLTQDYFEALSKTHLKAKISEHQGKIDIVFDVRHQKDYRILEE
                     LMLFGERLSEIKIRKNNSVFK"
     gene            13137..13409
                     /locus_tag="VGT87_001150"
     CDS             13137..13409
                     /locus_tag="VGT87_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEQ3814005.1"
                     /translation="MRLDKYLKVSRLIKRRSVAKEVADKGRIKVNGILAKSSTNIKLN
                     DHIEISFGNKLLTVRVIEIKDSTKKEDALKMYEIISETRITLNEEA"
     gene            13396..13767
                     /locus_tag="VGT87_001151"
     CDS             13396..13767
                     /locus_tag="VGT87_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEQ3814006.1"
                     /translation="MKKPSIVQLNNHYIKKENLKKKFEEEESQKRNRFMGWILVSMMF
                     LFILPTYNLVKSYVDFEKQNQQVVKLKKEYNELSKRTKKEKQLAERLKDDNFVKKYAR
                     AKYYLSREGEMIYPIPGLLPK"
BASE COUNT         4759 a         2220 c         2544 g         4420 t
ORIGIN      
        1 aagttttgga ggatatctcc ttaacactct tactattata gcagattgat tttttttgtc
       61 tatacttttt ttaaaaaaaa tttgattttt tttaaaccgc aagttgtcgc caaatttcaa
      121 gttgaagtta gccagtttta acatttctac tggcgactta tagttgaaga gtcgtttagg
      181 ataatgattg atccaatgtt caatagcagc gacttcttta ggggtcgttg ttttggtacc
      241 tttagggaga catctgcgaa tcaggcgatt atggttttcg ttagtgcctc tctcccatga
      301 cgcataggga tgggcatagt agatgtcact tgctggaaag acatctgata agcgagaaaa
      361 ttctgtcccg ttgtctgccg tgatagagtt gacggtgtag tcttttagaa tccccgtaag
      421 agctttattg acagcttgag cagacttatc cggtattaat cgaatcagct gatgacgtgt
      481 tttcctgtcc gttagggtga gtaagcattg atttttagcc ctcgtcagaa tgactgtatc
      541 aatctcataa tgcccagctt caagacgttg gttaatgcta tctgggcgtt gttcaatact
      601 ctttcctgcg ggtttaaaat aaggactggc atgttttcta gcctgcttag cctttctcgg
      661 ataaagcata gcctccttac caagacttaa tttcccatga tgaatccagt aatagatggt
      721 agatacaggg acttgaactc ccttagcttt aactatcatc tcaggggaat agttctgacg
      781 catgtagtgt tcaatagcct ccctatcttg cttacgctgt ctaatgggtc taacagaacg
      841 cttgcgattt tcttgataga ccaactgagc atgttgagca gaataaacgg cttcaaactt
      901 tcctttacga acctgttgcc taaccaatcc tcgcttgatt tcattatgaa tggtctgggg
      961 ggccttacct agtaagcgag caatttcacg attagagctt ccagcgttga atttgccgac
     1021 gctcatggat tgtcaattgt ttgccttttg gtgtataata atcttgcatc tttttgagtt
     1081 tccttgtgtt tgtggtgaac tacaagtata cctcttagag gtgctttttt cataccttaa
     1141 aagtggctaa gttcatttta gaatttttca acgcgggata taatgttatt ttctagtttt
     1201 ttcaaaataa atattgacag ttttagataa tttttgttaa aatgatgtgg ttaaggtctc
     1261 atagctcagc tggatagagc attcgccttc taagcgaacg gtcgcaggtt cgaatcctgc
     1321 tgggatcaaa gataaaaaag cactgtttta gtgctttttt tgtatttacc ttagtgtatt
     1381 gaaaagatag gatttagtct gtcttttttt aatgtttgct tttaattata ccgaactatt
     1441 atctaataaa gtggtaagag ttagttattc cagttcacac tctcttactt atgataagga
     1501 ctgccttgtg taatcataaa agcacgataa atttgctcag taagaactag acgcattaac
     1561 tgatgaggta aggttaacaa tccaaaactc attagcatat tagctctttt tttaataatt
     1621 gaatctaacc caagacttcc accaataata aacgtgatag ttgaatatcc cttaacagta
     1681 acccctgata ttaattcact aaatgtctct gaaggaaact gttttccttc aatagctaat
     1741 gcaattacaa aatcacgttc cccaattttt ttatggattc gctgagcttc tttactcata
     1801 atcaattgat tatcagcaaa acttgcttta tctggtgttc tttcatcagt taactcaatc
     1861 atttcgaatt gacaaaaacg ggaaagtctt ttttgatatt ctgaaatacc atctttgagg
     1921 tatctttctt ttagttttcc aacacaaatt agttttactt tcatagctta attgtatcac
     1981 aagaacatta ctttttacac aatttatcca caagttattt ttcttcaact actaataaaa
     2041 acctttgtac aaaggtcttt attagtaaaa agaaaagtaa tccacagcct gtggattact
     2101 tttctttttt ttatttttaa ggtataatta agcaaagttc tataagctat ggacaaataa
     2161 ttcggaggac aaaacatgcc aagtatgaaa catatcttaa aatccttaag tattttacta
     2221 gttggatttt taggaggatt aatagcaatt attacattta ataatctcta cccacattct
     2281 ccttcaaaaa taaattcagg taaggcaaca actagtaata tggtttttaa caacacaacc
     2341 aatactacta aagctgtcaa agctgttcaa aatgcagttg tatcagttat taattatcaa
     2401 gataaccctt cttcatccct ttctaaccct tatacaaaac tctttggaga agggcgttca
     2461 aaagagaata aggatgctga attatctatt tttagtgaag gatctggggt catttatcga
     2521 aaagatggca actccgctta cgttgttact aataaccatg ttatcgacgg agctaaacgg
     2581 attgaaattc ttatggcaga cggatctaaa gttgttggtg aattagttgg agctgatact
     2641 tattcggatt tagctgttgt taggatctct tcagataaga taaaaacagt agctgaattt
     2701 gcagattcta caaaactaaa tgttggagaa gttgctattg ctatcggcag cccactagga
     2761 acacaatacg ctaattctgt tactcaagga atcgtctcta gtttaagtcg tactgtaact
     2821 ttaaaaaacg agaatggtga gactgtctca acaaatgcta ttcagacaga tgctgctatt
     2881 aaccctggaa actctggtgg accactaatt aatattgagg ggcaagtaat cggaattaat
     2941 tcaagtaaaa tttcttctac cccaactggt agcaacggta atagtggtgc tgttgaagga
     3001 attggttttg ctatcccatc tactgatgtt attaaaatta ttaaacaact agaaactaat
     3061 ggtgaagtta tcagacctgc tcttggaata tccatggtca atttaaatga cttatccaca
     3121 aatgctctta gccaaattaa tattccaact agtgtaactg gtggtattgt agtagcagaa
     3181 gttaaggaag gaatgccggc atctggcaaa cttgctcagt acgatgtgat tacagaaatt
     3241 gatggcaaaa tagttaattc aattagtgat ttacaaagta gtctatacgg acacgatatt
     3301 aatgatacta ttaaagtaac tttttataga ggaactacaa agaaaaaagc agatatcaaa
     3361 ttaacaaaaa ctactcaaga tttgactaaa acgcagtaaa caagctacct aaatactatt
     3421 acattttatc acataaaaca gtccaaagga ctgttttatg tttcttattc tatccaggaa
     3481 ctcccttatg acaaaagaat tattgataga cctaccaatt gaagatattg ttacaaatcc
     3541 ctatcagcca aggatacaat ttaatcaaag agaactccaa gacctagcaa cttctattaa
     3601 atcaaacggc ctcattcaac ctattattgt tcgaaaatct gacatttttg gctatgagtt
     3661 agttgctgga gaacgacgac ttaaagcttc aaaaatggct ggtctaaaaa aaattccagc
     3721 tatcatcaag aagatctcta cactcgagag catgcaacaa gctatagttg aaaatttaca
     3781 acgttctaac cttaacgcta tcgaagaagc taaagcctat cagttattgg ttgaaaaaaa
     3841 acacatgact cacgatgaga ttgctaaata tatgggaaaa tcaagacctt atattagcaa
     3901 taccttacgt ctgttacaac tcccagcacc tatcattaaa gcaattgaag aaggaaaaat
     3961 tagtgcggga cacgcgcgtg ctcttttaac tttgagtgat gataagcaac aactgtacct
     4021 cactcataaa atacaaaatg aaggcctaag tgttaggcaa attgagcaac tggttacttc
     4081 tactccaagt tcgaagctat ctaaaaaaac taaaaatatt tttgccactt ctttagagaa
     4141 acaattggct aaatcattgg gactctctgt caatatgaag ctgacagcaa accatagtgg
     4201 gtaccttcag atatcttttt ccaatgatga tgaattaaac agaattatca acaagctaaa
     4261 gtagcttgtt gatattctgt tttttctttt ttagttttcc acataaaaaa tagttgaaaa
     4321 caatagcggt gtcaccttaa aatggctttt ccccaggttg tggagaaccc aaattaacag
     4381 tgttaattta ttttccacag gttgtggaaa actagaatag tttatggtag aatagttcta
     4441 gaattatcca caagaaggaa cctagtatga ctgaaaatga acaaattttt tggaacaggg
     4501 tcttggaatt agctcagagt caattaaaac aggcaactta tgaatttttt gttcatgatg
     4561 cccgtctatt aaaggtcgat aagcatattg caactattta cttagatcaa atgaaagaac
     4621 tcttttggga aaaaaatctt aaagatgtta ttcttactgc tggttttgaa gtttataacg
     4681 ctcaaatttc tgttgactat gttttcgaag aagacctaat gattgagcaa aatcagacca
     4741 aaatcaatca aaaacctaag cagcaagcct taaattcttt gcctactgtt acttcagatt
     4801 taaactcgaa atatagtttt gaaaacttta ttcaaggaga tgaaaatcgt tgggctgttg
     4861 ctgcttcaat agcagtagct aatactcctg gaactaccta taatcctttg tttatttggg
     4921 gtggccctgg gcttggaaaa acccatttat taaatgctat tggtaattct gtactattag
     4981 aaaatccaaa tgctcgaatt aaatatatca cagctgaaaa ctttattaat gagtttgtta
     5041 tccatattcg ccttgatacc atggatgaat tgaaagaaaa atttcgtaat ttagatttac
     5101 tccttattga tgatatccaa tctttagcta aaaaaacgct ctctggaaca caagaagagt
     5161 tctttaatac ttttaatgca cttcataata ataacaaaca aattgtccta acaagcgacc
     5221 gtacaccaga tcatctcaat gatttagaag atcgattagt tactcgtttt aaatggggat
     5281 taacagtcaa tatcacacct cctgattttg aaacacgagt ggctattttg acaaataaaa
     5341 ttcaagaata taactttatt tttcctcaag ataccattga gtatttggct ggtcaatttg
     5401 attctaatgt cagagattta gaaggtgcct taaaagatat tagtctggtt gctaatttca
     5461 aacaaattga cacgattacc gttgacattg ctgccgaagc tattcgcgcc agaaagcaag
     5521 atggacctaa aatgacagtt attcccatcg aagaaattca agcgcaagtt ggaaaatttt
     5581 acggtgttac cgtcaaagaa attaaagcta ctaaacgaac acaaaatatt gttttagcaa
     5641 gacaagtagc tatgttttta gcacgtgaaa tgacagataa cagtcttcct aaaattggaa
     5701 aagaatttgg tggcagagac cattcaacag tactccatgc ctataataaa atcaaaaaca
     5761 tgatcagcca ggacgaaagc cttaggatcg aaattgaaac cataaaaaac aaaattaaat
     5821 aacatgtgga aaagaatatc ttttatgaaa tagttatcca caagttgtga acaaccattt
     5881 agtcttggat tctctcgttt atttagagtt atccactata tacacaagac ctactactac
     5941 tacttattat tatacttatt aaataaagga gttctcatga ttcaattttc aattaatcgc
     6001 acattattta ttcatgcttt aaatgcaact aaacgtgcta ttagcactaa aaatgccatt
     6061 cctattcttt catcaataaa aattgaagtc acttctacag gagtaacttt aacagggtct
     6121 aacggtcaaa tatcaattga aaacactatt cctgtaagta atgaaaatgc tggtttgcta
     6181 attacctctc caggagctat tttattagaa gctagttttt ttattaatat tatttcaagt
     6241 ttgccagata ttagtataaa tgttaaagaa attgaacaac accaagttgt tttaaccagt
     6301 ggtaaatcag agattacctt aaaaggaaaa gatgttgacc agtatcctcg tctacaagaa
     6361 gtatcaacag aaaatccttt gattttaaaa acaaaattat tgaagtctat tattgctgaa
     6421 acagcttttg cagccagttt acaagaaagt cgtcctattt taacaggagt tcatattgta
     6481 ttaagcaatc ataaagattt taaagcagta gcgactgact ctcatcgtat gagccaacgt
     6541 ttaatcactt tggacaatac ttcagcagat tttgatgtgg ttattccaag taaatctttg
     6601 agagaatttt cagcagtatt tacagatgat attgagaccg ttgaggtatt tttctcacca
     6661 agccaaatct tgttcagaag tgaacacatt tctttttata cacgcctctt agaaggaaat
     6721 tatcccgata cagaccgttt attaatgaca gaatttgaga cggaggttgt tttcaatacc
     6781 caatcccttc gccacgctat ggaacgtgcc ttcttgattt ctaatgctac tcaaaatggt
     6841 actgttaagc ttgagattac tcaaaatcat atttcagctc atgttaactc acctgaggtt
     6901 ggtaaggtaa acgaggattt agatattgtt agtcagtctg gtagtgattt aactatcagc
     6961 ttcaatccaa cttaccttat tgagtcttta aaagctatta aaagtgaaac agtaaaaatt
     7021 catttcttat caccagttcg accattcacc ctaacaccag gcgatgagga agaaagtttt
     7081 atccaattaa ttacaccagt acgaacaaac taagtaagaa aaagctccct tttaggagtt
     7141 tttttgttat tataaatatt aacgataata taagtggaga aaagggaatg tatcaaattg
     7201 gatcatttgt tgaaatgaaa aaacctcatg cttgtgtaat taaagaaact ggtaaaaagg
     7261 ctaatcaatg gaaggtactg agagtaggag ctgatattaa aattcagtgt actaactgtc
     7321 agcatgtgat tatgatgagt cgttatgatt ttgagcgaaa attaaaaaaa gtactgcaac
     7381 catgaaagtc cttttaaata aataattcta gctatatttg caaccaatac tttcctaaaa
     7441 aattgctagt atgccgttgg aaaattagct attctaatgt tatcgaaaga agaaagatgg
     7501 ccattgacaa tgacgaaagt tgtagcacag ttacttagtt tctcggcaaa ctatatcaaa
     7561 atgagaataa tacaccaaac tttgataatt taaatgaagg tgaaagagtg gcataatgat
     7621 aagcaaatct gaaattttct tcttattcat accgcttttt cttctatttt ttggtataat
     7681 aatcttgatt gaaatttgaa tggagattcg ctaaatggct ttaacagcag gtattgtagg
     7741 cttaccaaat gttggtaaat caaccttatt taacgcaatt acaaaagcag gagcagaagc
     7801 tgctaattat ccttttgcga ctattgatcc caatgttggt atggtagagg ttccagatga
     7861 gcgtctgcaa aaattgacag aattgattac acctaaaaaa acagttccga caacctttga
     7921 atttactgat attgcaggta tcgttaaagg tgcttctaga ggggaaggtc taggaaataa
     7981 atttttagct aatattcgtg aagtcgatgc tattgttcat gtggtacgtg cttttgatga
     8041 tgaaaatgtc atgcgtgaac aaggtcgtga ggatgctttc gttgatccta tagcagatat
     8101 tgacactatt aatcttgaat taatcttagc tgatttagag tcaatcaata aacgttatgc
     8161 gcgtgttgaa aaaatggcac gaactcaaaa agataaagaa tcagtagcag agttcaatgt
     8221 tttgcaaaag attaaacctg ttttggaaga tgggaaatca gctaggacaa ttgagtttac
     8281 agaagatgaa gcaaaagttg ttaaaggtct ctttttatta acaactaaac ctgttttata
     8341 tgtagctaat gtcgatgaag ataaagttgc taatccagat ggtattgatt atgtcaaaca
     8401 aattcgtgac tttgcagcta ctgaaaatgc tgaagtagtt gttatctcag cgcgtgcaga
     8461 agaagaaatt tcagagcttg acgatgagga taaagaagaa tttttggaag ctatcggtct
     8521 tactgaatca ggcgttgata aattaaccag agcagcttat catctcttag gccttggaac
     8581 ctattttaca gcaggtgaaa aagaggttcg tgcttggacg tttaagcgtg gtataaaagc
     8641 tccacaagct gctggtatta tccattcaga ttttgaaaga ggctttattc gtgcagtaac
     8701 catgtcttat gatgatctaa tgacctacgg ttcagaaaaa gccgtcaaag aagctggacg
     8761 cttgcgtgaa gaaggaaaag aatatgttgt ccaagatggg gacatcatgg aattcagatt
     8821 taatgtgtaa ttatattaaa acaatatcag aaggttggaa gaacattcca gcccttttgg
     8881 cattttagaa agagaaaata tggtaaaaat gattgttggt ctgggaaatc caggctctaa
     8941 atatgaaaaa acaaagcaca atattggttt tatggctatt gacaatattg tcaagaacct
     9001 tgacgttacc tttacagatg ataaaaattt taaagcacag ataggaagta cttttattaa
     9061 tcatgaaaaa gtttactttg tgaaacctac tacttttatg aataatagcg gcatagcagt
     9121 aaaagcatta ctaacctact ataatattga cataacagat ttaattgtta tctatgatga
     9181 tttagacatg gaagtcagta aattacgttt acgtagtaag ggttcagcag gaggacataa
     9241 tggcattaag tcaatcattg cccacattgg aactcaggaa tttaaccgaa tcaaagttgg
     9301 tattggacga cctttaaaag gtatgactgt tattaaccat gtgatgggcc aattcaatac
     9361 tgaagataat attgctattt cgttaactct tgacagagtt gtcaatgctg tcaagtttta
     9421 tttacaagaa aatgattttg aaaaaacaat gcagaaattt aatggataat catggatatt
     9481 ttagaattat ttagtcagaa taagaaagtc caatcctggc actctggatt aaccacctta
     9541 ggaagacaac tggtaatggg gttatcgggt tcaagtaaag cattggctat agcttccgct
     9601 tatttagatg atcaaaaaaa aatagttgtg gttacatcaa ctcaaaatga ggttgaaaaa
     9661 ttagccagcg atttatctag tttacttgat gaagaacttg ttttccaatt ttttgcagac
     9721 gatgtggctg cagcggaatt tatctttgcg tcaatggata aagctctatc aagaatagaa
     9781 accctgcaat ttttaaggaa tcctaaatct cagggcgttt taattgttag tttatcaggc
     9841 ttaagaactt tattgccaaa cccagatgtt tttacaaaga gtcagattca actaacagtt
     9901 ggagaaaatt atgatagtga tactcttact aaacaactga tgacaattgg atatcagaag
     9961 gtctcacagg ttattagtcc gggagaattt agccgtcgag gggatatttt agatatctat
    10021 gagattacac aagaattgcc ttatcgattg gaattttttg gtgatgatat tgatagtatt
    10081 aggcaatttc atccagaaac tcaaaaatct tttgaacaac tagaaggtat ttttattaat
    10141 ccagcaagtg atcttatttt tgaggctagt gattttcaac gtggcattga gcaattagaa
    10201 aaggctctac aaacagcaca agatgataaa aaatcttatt tagaagatgt attagctgtt
    10261 tcaaaaaacg gttttaaaca taaggatatc cgtaaatttc aatcattatt ttatgaaaaa
    10321 gagtggtcat tattagatta tattcctaag ggaacgccaa tcttttttga tgattttcaa
    10381 aaactagttg ataaaaatgc aagatttgat ttagagattg ctaatctctt gacagaagat
    10441 ttacagcaag gaaaggctct ttccaatctt aactacttta cagataatta tcgagagctt
    10501 aggcactata agccagcgac cttcttttca aattttcata agggacttgg aaatatcaaa
    10561 tttgatcaga tgcatcagct aactcagtat gccatgcagg aattttttaa tcaatttcct
    10621 ttgttgattg atgagattaa acggtatcaa aaaaatcaaa caacggttat tgtacaggta
    10681 gagtctcagt atgcttacga acgacttgaa aaatcttttc aagattacca atttcgcctt
    10741 cctttagtga gtgctaatca aattgtttca cgtgaatcac aaattgtaat tggagctatc
    10801 tccagtggtt tttattttgc tgatgaaaag ttagcgttaa tcacagagcg tgaaatttat
    10861 cataaaaaga tcaaacgacg cgctagacga tctaatatta gcaatgctga gcgtttgaaa
    10921 gattacaatg agttagcagt aggtgattac gtggttcata atgtccatgg tatcggtcgc
    10981 tttcttggaa ttgaaacgat tcagattcag ggaatccatc gagattatgt tactattcaa
    11041 tatcaaaatt cagaccgtat ttctcttcca attgaccaaa ttggtagctt atcaaagtac
    11101 gtttctgctg atggaaaaga acctaaaatt aataaactca atgatggtcg ttttcaaaag
    11161 ataaagcaaa aggtcgctag acaagtagag gatattgctg atgaccttct aaaattatat
    11221 gctgaaagaa gtcagcaaaa aggattttca ttttcaccag atgatgactt gcagcgcgct
    11281 tttgatgatg attttgcttt tgtagaaaca gaagatcaac ttaggtctat aaaggaaatt
    11341 aaagctgata tggagagcat gcagcctatg gatcgtcttt tagtaggcga tgtaggattt
    11401 ggtaagacag aagtagctat gagggcagcc tttaaagcgg tgaatgatca caaacaagta
    11461 gctgtcttag ttccaaccac agtcttggcc cagcagcatt atgaaaactt caaagcacgc
    11521 tttgaaaatt accctgttga ggttgacgtc ttaagtcgtt tccgtagtaa aaaagagcaa
    11581 gctgaaacac tagaacgtgt acgaaaaggt caaattgaca ttattatcgg aacccatcga
    11641 ctcctatcaa aagatgtggt cttttctgat ttaggattga ttgtgattga tgaggaacaa
    11701 cggtttggtg ttaagcataa agaaacttta aaggaattaa aaactaaggt tgacgtctta
    11761 accttaacag ctactccgat tcctagaacc ttacacatgt ctatgttagg tatccgagat
    11821 ttatcggtta ttgagacccc accaaccaat cgttatcctg ttcaaaccta tgttttggaa
    11881 aataatccag gtctcgttag agaagctatc attcgtgaaa tggatcgtgg aggacaaatt
    11941 ttttacgttt acaataaagt tgacactatt gaaaagaaag ttgcagagct acaagaatta
    12001 gtcccagaag cttctatagg ttttgttcat gggcaaatga gtgaaattca acttgaaaat
    12061 accttgattg actttataaa tggtgattat gatatccttg tggctacaac aatcatcgaa
    12121 acaggagttg acatttctaa tgtaaacact ttgtttattg agaatgctga tcatatggga
    12181 ttgtcaacat tgtatcaact gagggggcgc gtcggaagaa gtaatcgtat tgcctatgct
    12241 tacctgatgt atcgtcctga taaggtccta acagaagtct ctgaaaaacg tttagaggct
    12301 attaaaggct ttactgaatt aggttcaggc ttcaagattg ctatgcgaga tttgtctata
    12361 cgaggagcag gtaatatttt aggagcttct caaagtggct ttattgattc ggtcggtttt
    12421 gaaatgtatt ctcagttatt ggaacaggct attgctagca agcaaggaaa aacgactgtt
    12481 cgccaaaaag gtaatactga aatcaatctt cagattgatg cctatttacc agatgattat
    12541 attgcagatg agcgccaaaa aattgacatt tacaagcgta ttcgagaaat tcgatcaaga
    12601 gaagattatc tcaatttgca agatgagctt atggatcgtt ttggagagta tcctgatcaa
    12661 gttgcctatt tgttagagat agctctgcta aaacattata tggacaatgc ctttgcggaa
    12721 ttagttgaac gcaaaaataa tcaggtcatt gttcgatttg aagtaacttc tttaacttat
    12781 ttcttgactc aggattattt cgaagcctta tcgaaaaccc atcttaaagc taaaattagt
    12841 gaacatcagg ggaaaatcga tatcgtcttt gacgttcgcc atcaaaaaga ttatagaatt
    12901 ttagaagaat tgatgttatt tggagaaagg cttagtgaga taaaaatcag aaaaaacaat
    12961 tcagttttta aataataatt tgaaacaact tttagactga atagtattaa tgacaatgga
    13021 acgaactagc tctttatact tttgtcaaaa atctaagatc tttgctgcga aattaggatc
    13081 ttttctacca tttaaaaaat aaaaatgata aaatgaagag gattgtagga gaaattatga
    13141 gactagataa atatctaaag gtatcgcgcc ttattaaacg tcgttcagta gcaaaagaag
    13201 ttgcggataa gggacgaatt aaagttaatg ggatacttgc taaaagttca acgaatataa
    13261 aactaaatga tcacattgaa attagttttg gaaataaatt actgacagta agagtcattg
    13321 aaataaaaga tagtacaaaa aaagaagatg ctcttaagat gtatgagata atcagtgaaa
    13381 caaggataac attaaatgaa gaagcctagt attgttcaat taaataacca ttatattaag
    13441 aaagagaatc tcaaaaaaaa atttgaagaa gaagaatctc aaaaaagaaa tcgttttatg
    13501 ggatggatcc ttgtaagtat gatgttttta tttattttgc caacttataa tcttgtcaaa
    13561 agttatgttg attttgaaaa gcaaaatcaa caggtggtta aattaaaaaa agagtataat
    13621 gaattgtcaa agaggacaaa aaaagaaaaa caattagcag aacgactaaa agatgataat
    13681 tttgtcaaaa aatatgctag ggcaaaatac tatttatcgc gtgaaggaga aatgatttat
    13741 cctattccag gactattacc aaaatgatga tggacaatat tgtaaaaaaa atagaagcat
    13801 ttcttgcttt ttctgataaa aaattagcag agctgcaaca ggaaaatcaa aaagttaaag
    13861 aagaaagtca gtatattcaa aaaaactaga aaggagtcaa tatgagaaaa ttattagcgg
    13921 ctatgttaat gacttttttt ctg
//
LOCUS       DAUCLR010000047        13928 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.047, whole genome shotgun sequence.
ACCESSION   DAUCLR010000047 DAUCLR010000000
VERSION     DAUCLR010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13928
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.047"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..215
                     /locus_tag="VGT87_001152"
     CDS             <1..215
                     /locus_tag="VGT87_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease
                     C-terminal domain-containing protein"
                     /protein_id="HEQ3814007.1"
                     /translation="LLIKLIQIFEELSFVTIDDGLMTVNPQAQKREISESHIYQDLKE
                     LVKFQEIMALASPKEMYDYLVKSNDQ"
     gene            365..883
                     /locus_tag="VGT87_001153"
     CDS             365..883
                     /locus_tag="VGT87_001153"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEQ3814008.1"
                     /translation="MDLTNYIASIKDYPKAGITFRDISPLMADGKAYSYAIREIAQYA
                     CDKDIDMVVGPEARGFIIGCPVAVELGIGFAPVRKPGKLPRDVVSADYEKEYGLDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGIVAGCAFLIELEGLNGRHAIR
                     NYDYKVLMQFPG"
     gene            964..1647
                     /locus_tag="VGT87_001154"
     CDS             964..1647
                     /locus_tag="VGT87_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEQ3814009.1"
                     /translation="MSFLEHYKSGNLVIPSALLFHYKDLFKSSDDFLVWQFFYLQNTT
                     KRDDLAPSQIAHALGKSVADINKIISSLTSQGLLDMRTIELTGEIEIIFDASPVLAKL
                     DQLFVSQTATEIDKQETPNHFKRLVDEFERELGRFLSPFELEDLEKTLRDDKTDPDLI
                     REALKEAVFNGKTNWKYIQAILRNWRKEGIVNLRQVEERRRVREGEDLSQVTISEDFL
                     SAMNLWSDS"
     gene            1644..2300
                     /gene="nth"
                     /locus_tag="VGT87_001155"
     CDS             1644..2300
                     /gene="nth"
                     /locus_tag="VGT87_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEQ3814010.1"
                     /translation="MRIGKARLAKVLTIIGQMFPEAKGELDWETPFQLLIAVILSAQT
                     TDKAVNKVTPGLWQSYPEIEDLAFAELSDVENALRTIGLYKNKAKNIIKTAQAIRDDF
                     KGQVPKTHKELESLPGVGRKTANVVLAEVYGVPAIAVDTHVARVSKRLNISSPDADVK
                     QIEADLMAKIPKKDWIITHHRLIFFGRYHCLAKKPKCEICPVQSYCKYYQNTYGKSKA
                     "
     gene            2372..3058
                     /locus_tag="VGT87_001156"
     CDS             2372..3058
                     /locus_tag="VGT87_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEQ3814011.1"
                     /translation="MDSQLSNRLAQVAAYVPKGVKLLDVGSDHAYLPIFLVETNQISA
                     AIAGEVVRGPYESALKNVTQSGLAEHIQVRLANGLAAFEEADDVTAITICGMGGRLIA
                     DILEAGKEKLQGIERLVLQPNNREDDLRAWLSVNAFKIVAETIMAENDKYYEIIVAEH
                     GEKALSATELRFGPYLSQEKSVVFKEKWQREMDKLAYALSCIPEEKTQERQLLLTKIQ
                     QIKEVIVHES"
     gene            3048..3836
                     /locus_tag="VGT87_001157"
     CDS             3048..3836
                     /locus_tag="VGT87_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019781659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEQ3814012.1"
                     /translation="MKAKTLIDAYEAFCPLDLSMEGDVKGLQMGSLDKDIRKVMITLD
                     IRESTVAEAIKNEVDLIITKHAPIFKPLKDLVSSPQRDILLDLVKHDISVYVSHTNID
                     IVPGGLNDWFCDLLEIKEATYLSETKEGFGIGRIGTVKEQALEELASKVKRVFDLDTV
                     RLIRYDKENPLISKIAICGGSGGEFYQDAVQKGADVYITGDIYYHTAQEMLTEGLFAV
                     DPGHHIEVLFTEKLKEKLQGWKEENGWDVSIISSKASTNPFSHL"
     gene            3876..4982
                     /locus_tag="VGT87_001158"
     CDS             3876..4982
                     /locus_tag="VGT87_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEQ3814013.1"
                     /translation="MKIAIIGAGIVGSTAAYYLQQSGQKEVTIFDHGQGQATKAAAGI
                     ISPWFSKRRNKVWYRMARLGADFYQQLINDLKEDGFATDFYQQNGIYVLKKQEEKLRD
                     LYELALARKVESPIIGELAIKNRKELGNDFKGLIGFDNCLYASGAARVEGAALCETLL
                     KASGYPVIRQKVTLKQQGSGYEIAGHYFDQVILAAGAWLPDLLRPLGYQVDVRPQKGQ
                     LLDYDVHHIISDTYPVVMPEGEIDLIPFNQGKISVGASHENDKGYDLEPDWQVLEKIE
                     MQALTYLPLLKEATQKSCRVGIRAYTSDYSPFYGQVPGLKNLYTASGLGSSGLTVGPL
                     IGYELAQLLLGHEGLLTPSDYSPEPYLLRSTGKA"
     gene            5040..5909
                     /gene="rfbA"
                     /locus_tag="VGT87_001159"
     CDS             5040..5909
                     /gene="rfbA"
                     /locus_tag="VGT87_001159"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEQ3814014.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPIYDKPMIYYPLSTLMLA
                     GIKDILIISTPQDLPRFEELLGDGSEFGISLSYKEQPSPDGLAQAFIIGEEFIGDDRV
                     ALILGDNIYHGNGLTKMLQKAAAKEKGATVFGYQVKDPERFGVVEFDENMNAISIEEK
                     PEVPKSHFAVTGLYFYDNDVVEIAKNIKPSARGELEITDVNKAYLDRGDLSVELMGRG
                     FAWLDTGTHESLLEAAQYIETVQRLQNAQVANLEEIAYRMGYISKEDVHKLAQSLKKN
                     EYGQYLLRLIGEA"
     gene            5909..6502
                     /locus_tag="VGT87_001160"
     CDS             5909..6502
                     /locus_tag="VGT87_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEQ3814015.1"
                     /translation="MTETFFDKPLACREIKEIPGLLEFDIPVRGDNRGWFKENFQKEK
                     MLPIGFPERFFEEGKLQNNVSFSRQHVLRGLHAEPWDKYISVADDGKVLGAWVDLREG
                     ETFGNVYQTVIDASKGMFVPRGVANGFQVLSETVSYSYLVNDYWALDLKPKYAFVNYA
                     DPSLGITWENLAAAEVSEADKNHPLLSDVKPLKPKDL"
     gene            6746..7786
                     /gene="rfbB"
                     /locus_tag="VGT87_001161"
     CDS             6746..7786
                     /gene="rfbB"
                     /locus_tag="VGT87_001161"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEQ3814016.1"
                     /translation="MYKNIIVTGGAGFIGSNFVHYVYNNHPDVHVTVLDKLTYAGNRA
                     NIEAILGDRVELVVGDIADAELVDKLAAKADAIVHYAAESHNDNSLEDPSPFIHTNFI
                     GTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGQGEGPGEKFTAETKYNPSSPYS
                     STKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILAGIKPKLYGE
                     GKNVRDWIHTNDHSTGVWAILTKGRIGETYLIGADGEKNNKEVLELILEKMGQPKDAY
                     DHVTDRAGHDLRYAIDSTKLREELGWEPQFTNFSEGLEETIKWYTENETWWKAEKDAV
                     EAKYAKTQEVIK"
     gene            complement(7869..9008)
                     /locus_tag="VGT87_001162"
     CDS             complement(7869..9008)
                     /locus_tag="VGT87_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEQ3814017.1"
                     /translation="MWSEKHKSGKVNFVERYKHPYTGKWCRASVLMEKDTPRIRKEAQ
                     KYLDEKISKILTSLTTTDAYLLDVMNEWWEHHQKSLKSTSVRSLDFRIRELRNLIDPE
                     VMIAKITTKYLQSIIDKIPGSYDKRKRARQLLKQTFDYAIALEYVSINPVISTQLAKP
                     VKTIKDFEDVAQKFLEKDELKRLLDEMYRRKGSIKMAYLAEFMSLNGCRIGEALAIQP
                     DNIKNDIIEIHGTLDYTSNGYRNAIKTTPKTNSSWRETLITKREKEIIQDILKINALE
                     KNTNPNYKDMGYIFISRNGVPIQDNALNTSIRAANKRLEKPIQKELTSHIFRHTLVSR
                     LAENKVPLKTIMDRVGHADSKTTQQIYTHVTKSMKNEVVDILNRL"
     gene            complement(9128..9985)
                     /locus_tag="VGT87_001163"
     CDS             complement(9128..9985)
                     /locus_tag="VGT87_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEQ3814018.1"
                     /translation="MKNISPLRYPGGKSQVYDYVRELVIANDAITYIEPYMGGMGIAL
                     KLLLNNNVHKIMVNDYDKAIYAFWYSVLNYTEQLIEKINTTPITIDEWKLQREVQKNK
                     NNCDDLLTLGFSTLFLNRTNRSGIIKAGVIGGLKQDGNYKLDCRFNKEKTIKKIKLIA
                     SYKKQIKLYNMDAEKFIRLNITKTKNSFTFFDPPYYTKGPGLYTNFYNHENHHSLSST
                     IKHYMSNKNWILTYDLSEEIFQMYKEFKYEKYYLNYSVTKPSKGIEYIFYSNGLAVPE
                     NTINIKKAN"
     gene            complement(10024..11688)
                     /locus_tag="VGT87_001164"
     CDS             complement(10024..11688)
                     /locus_tag="VGT87_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEQ3814019.1"
                     /translation="MEKIEFIQYRKLKNITIDFDKNINIIAGTNGTCKSSILHLISNS
                     HQRVSNSSRNPILSTIQKMNKIFNPKIESLARGDKKYNDPAPGMTGTLYTAFYENYSL
                     DFRRHNSRTVNNTERFAIKPKYKRGTSDRLPEIPIIYLGMFRLFSYGEWEGESKFHNI
                     INKLPKQYLDQLSMLYKKFTGYEIKFDGKLNNLGGIRSKTEFSTNTEGIDSNTISAGE
                     DNLLTILISLVSLKAYYESITPDTEKTVESILLIDELDASLHPEFQIKLLEEFKTFSK
                     DYKIQIFFTTHSLSLIDYSLDKKIGNIIYLIDQIDHVDLLKHLNKYSINALLQNKLVN
                     NVYVKQPIPVLLEDNEAKDFFQNLLQAYFERYQLSLESFFHIIDAKISSSAIKALAQD
                     RYTNQSSLRIISITDGDEQLGQQYLTHNLFSLPGNYSPEELIFKYLKEVLLDDYNFWQ
                     KQDIIAAGYTKKLVQESFITQLDELEAKLNQPGSHHGVKREENKKIYNCDTNFWNYVM
                     KYWIDDTNNQDEVLKFFDNLKIAFHKTAPFYNINPTSSWPFITGQL"
     gene            complement(11690..12073)
                     /locus_tag="VGT87_001165"
     CDS             complement(11690..12073)
                     /locus_tag="VGT87_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEQ3814020.1"
                     /translation="MVKINEMLSKYNIKLFEFPDTMWKRSGFYYPDLRIIYVNQNLSQ
                     IEKEKVILHELGHIEHDPKQYQRLLLKYENQADRFMIRELIKNYLSSHNVVDFNWLQF
                     ATTYQISTTWGQQMIQEEFQNIVGV"
     gene            complement(12057..12398)
                     /locus_tag="VGT87_001166"
     CDS             complement(12057..12398)
                     /locus_tag="VGT87_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ3814021.1"
                     /translation="MDEKDLKRIIESRYNSVRAFAIENDIPYTTMRSILERGVMNAKA
                     ETIFKICDILGINPESFAEEKTDWQATIDLSNLREKVVMFDGKPLSDKDVEKIEAIIK
                     ISLGVGNGEDK"
     gene            12596..12808
                     /locus_tag="VGT87_001167"
     CDS             12596..12808
                     /locus_tag="VGT87_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEQ3814022.1"
                     /translation="MKNIEHIRKEKGVSLVDIADCLRLKSQTVREKINGDSDFKFGEA
                     LKVQQTFFPEFDIVYLFKERKESSVG"
     gene            12836..13555
                     /locus_tag="VGT87_001168"
     CDS             12836..13555
                     /locus_tag="VGT87_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HEQ3814023.1"
                     /translation="MNHLINVTLNENQEPVVSARDLHKELKVKTRFSEWVKQNFKILE
                     EGYDFTSVVGTTVVNNGAVREIQDYVLSLDAAKNLAMVSKTDKGKEVRKYFIQVEKDF
                     NSPEKIMARALLMADKKVHKLEAQIEADRPKVLFADAVEASETSILIGDFAKILRQNG
                     YNIGQNRLFAWLRENGFLIRKNGESYNMPTQRSMDMKLFEVKERTHQEPNGSIRISKT
                     TKMTGKGQQYFINKFLNEKVG"
     gene            13559..13726
                     /locus_tag="VGT87_001169"
                     /pseudo
     CDS             13559..13726
                     /locus_tag="VGT87_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054766.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4279 a         2321 c         2815 g         4513 t
ORIGIN      
        1 ttttactgat taagttgatt caaatttttg aagagttgtc ttttgtgacg attgacgatg
       61 ggctgatgac ggttaatccc caagcgcaaa aacgagagat ttcagagagc cacatttacc
      121 aagacttaaa agaattggtg aaatttcaag aaatcatggc tttggctagc ccgaaagaga
      181 tgtatgatta tttggtgaaa tcaaacgatc aataagtaaa gcagttgaca gtagttattg
      241 aggtagttac gaatgaagta tcagataaac ataatgtcag aagtcttatt ttacgaaaaa
      301 tatgtttcgt gatataatga agagtatttt atatttggca atttgccact agaaagaatt
      361 aactatggat ttaacaaatt acattgcatc gatcaaagat taccctaaag ctggtattac
      421 gtttcgagat atctcacctt taatggctga cggtaaagca tatagctatg ctattcgtga
      481 aatagcacag tacgcttgtg ataaagatat tgatatggtt gttggacctg aagcgcgtgg
      541 ttttatcatt ggttgtcctg ttgcagttga gttaggaatt ggttttgctc ccgttcgtaa
      601 accaggaaaa ctgccaagag atgttgtctc tgctgattac gaaaaagaat atggtcttga
      661 taccttaact atgcatgcag atgctattaa gcctggacaa cgtgttttga ttgtcgatga
      721 tttactagcg actggcggta cggtaaaagc aactattgaa atgattgaaa aacttggtgg
      781 tattgtagct ggttgtgcct tccttattga attagaaggt ttaaatggtc gccatgctat
      841 taggaactat gactataaag tcttaatgca gtttcctgga taagcacatt aatcagcatc
      901 tgattagggg atttaaagta gtaaaggcta tagattcgat tttagccttt tgattgaggg
      961 attatgagtt ttttagaaca ttataagtca ggtaatttgg ttatcccaag tgccttgctt
     1021 tttcattaca aagatctttt taagagctca gatgattttt tggtctggca gtttttctat
     1081 cttcaaaata cgacaaagcg agatgactta gcacccagtc aaattgctca tgcgcttgga
     1141 aaatcagttg ctgatattaa taaaatcatt tcatctttga ctagtcaagg gcttttagat
     1201 atgcggacaa ttgaacttac tggagaaatt gaaattattt ttgatgcgag tcctgtgtta
     1261 gccaagttag atcaattgtt tgtcagtcaa acggcaactg aaattgacaa acaagaaaca
     1321 ccaaaccatt tcaaacgatt agttgatgaa tttgaacgtg aattaggacg attcttgagt
     1381 ccttttgagt tagaagatct tgaaaaaacg cttcgtgatg ataaaacaga tcctgatttg
     1441 atacgagaag ctttaaaaga agctgtcttc aacggcaaaa caaattggaa atatatccag
     1501 gctatcttac gtaattggcg aaaagaaggt attgttaatc ttcgacaagt agaagaacgt
     1561 agacgtgtta gagaaggcga agatctttct caagtgacga tatcagagga ttttctatct
     1621 gcgatgaatt tatggagtga ttcatgagaa ttggaaaagc aagattagca aaagtcctga
     1681 ccatcattgg tcaaatgttt cctgaggcta agggagagtt agactgggaa acaccttttc
     1741 aattgttgat tgcggtgatt ttatcagctc aaacgacaga taaggccgtc aataaggtaa
     1801 ctcctggact atggcagtct tatccagaaa tagaagactt agcttttgct gaactttctg
     1861 atgttgaaaa tgctctgaga acgattggac tctataaaaa taaggccaaa aatatcatta
     1921 aaactgctca ggctattcgt gatgatttta agggtcaagt gccaaaaacc cacaaagagc
     1981 ttgaaagttt accgggtgtt ggccgaaaaa cagccaatgt ggtattggca gaggtttatg
     2041 gtgttccagc tattgcggtt gatactcatg tggctagagt ctcaaagagg ctcaatattt
     2101 catcaccaga tgccgatgtc aagcagattg aagcagattt gatggctaaa attccaaaga
     2161 aagattggat tattactcac catcgattga ttttctttgg acgctaccat tgtttagcca
     2221 aaaaaccaaa atgtgagatt tgtcccgttc agtcttactg caagtactat caaaatactt
     2281 atggaaaatc gaaagcctag cctttggttt tcttttttta tggaggtaat tttgctataa
     2341 tagaaccatt acgttttaga aagaaatcaa gatggatagt caattatcaa accgtttagc
     2401 ccaagttgcg gcctatgttc caaaaggtgt caaattatta gatgttggca gtgatcatgc
     2461 ctaccttccc atatttttgg tagaaactaa tcagatttca gcagccattg ctggcgaagt
     2521 ggttaggggt ccctacgaat ctgctcttaa aaatgtcaca cagtctggcc ttgccgagca
     2581 tattcaggtg cgcttagcca atggccttgc agcctttgaa gaagctgatg atgtgacagc
     2641 cattaccatt tgtggcatgg gtggccgttt gattgctgat attttagagg caggtaagga
     2701 aaaattgcag ggcattgaac gtctggtttt acaacccaac aatcgtgagg atgatttacg
     2761 ggcttggtta tcggttaacg cttttaaaat tgttgcagaa accatcatgg ctgaaaatga
     2821 caaatattat gaaatcatcg ttgctgagca tggtgaaaag gccttatccg cgacagaatt
     2881 gcgttttggt ccataccttt ctcaggaaaa atcagtcgtt tttaaagaga agtggcaacg
     2941 agaaatggat aagttagctt atgccttgtc ctgtatccca gaagaaaaaa ctcaagagcg
     3001 tcagcttctc ttgacaaaaa tacaacaaat aaaagaggtg attgttcatg aaagctaaaa
     3061 ccctaattga tgcctatgaa gccttttgcc cacttgattt atctatggaa ggagacgtta
     3121 aaggtcttca aatgggttca ttggataaag acattcgtaa ggttatgatt actttagata
     3181 tccgagagtc aacagtagca gaagccatca aaaatgaagt tgatctcatt ataactaagc
     3241 atgctcccat ctttaaaccg cttaaagatc tggtttcatc ccctcagcgt gatatccttc
     3301 tagatttggt caagcatgac atttctgtct atgtgagcca taccaatatt gacattgtac
     3361 caggtgggct aaatgattgg ttttgtgacc tgctagaaat caaggaagca acttacttgt
     3421 ctgaaaccaa agaaggcttt ggcattggtc gtattggaac agttaaagaa caagcgttag
     3481 aagagttagc aagtaaggtc aaaagggtgt ttgatttaga taccgtgcga cttatccgtt
     3541 acgataagga aaaccctttg atttcaaaga ttgctatctg tggcggaagt ggtggtgaat
     3601 tttatcagga tgctgtgcaa aaaggcgcag acgtttatat tacaggggat atctattacc
     3661 atacagcaca ggagatgctg acagaaggac tatttgcggt tgacccgggg catcatattg
     3721 aggtgctttt tacggagaaa ttaaaggaga aactccaagg gtggaaagaa gagaacggtt
     3781 gggatgtcag cattatttca agcaaagcct cgaccaatcc atttagccat ttataggctg
     3841 ttaaggtctt aaaataagat aaaaggatac caggaatgaa aatagccatt attggggcgg
     3901 ggattgtagg atcaaccgcc gcttattatt tacaacaaag tggtcaaaaa gaagttacca
     3961 tttttgacca tggtcaagga caagccacta aggctgctgc tggtattatt agtccttggt
     4021 tttcaaagcg tcgcaataaa gtttggtacc gcatggctcg tttaggagcg gacttttatc
     4081 agcaattaat caatgacctc aaagaagatg gttttgcaac agatttttat cagcaaaatg
     4141 gcatctatgt tttaaaaaag caggaagaaa aactaagaga tctttatgaa ttagcgttag
     4201 caagaaaggt agaatcaccg attattggtg agttagccat taaaaatcgt aaagaactgg
     4261 ggaatgattt taaaggattg attggctttg ataactgtct gtacgcttca ggagctgcta
     4321 gagtggaggg agccgcttta tgtgagacgt tactcaaggc tagtggttat ccagttattc
     4381 gccaaaaagt cactctgaaa cagcaaggaa gtggttatga gattgctggt cattattttg
     4441 atcaggtcat tctggcagca ggagcatggc tacctgactt attgaggcct ttaggctatc
     4501 aggttgatgt gcgaccacaa aagggacaac tcttggatta tgacgttcat cacatcatca
     4561 gcgacactta tccagttgtt atgccagaag gtgaaataga tcttattcct tttaaccaag
     4621 gtaagatttc tgttggtgcg agccatgaaa atgataaagg ttatgatttg gagcctgatt
     4681 ggcaggtctt agaaaaaatt gaaatgcaag ctctcactta tttgccgtta ttaaaagaag
     4741 ctactcaaaa atcatgtcgt gtagggataa gagcttatac cagtgattat tctccttttt
     4801 atggtcaggt tccaggattg aagaaccttt atacagctag tggtttaggg tcatcgggat
     4861 taacagttgg acctttgatt ggctatgaat tagctcaatt attgctagga cacgaaggac
     4921 ttttgacgcc tagcgactat tcccctgagc cttacctttt gcggtctact ggtaaagcgt
     4981 gacactgctt tcaaaaaatg atagaatgat agggtatata gaagaatagg agcactacta
     5041 tgaaaggtat tattcttgca ggaggttcag ggacacgctt gtatccactg acccgtgccg
     5101 catctaaaca gttgatgccc atttatgaca aaccaatgat ttattaccca ttgtcaacct
     5161 tgatgttggc agggattaaa gatattttaa tcatttcaac tccccaagat cttcctcgtt
     5221 tcgaagaatt gctaggagac gggtcagaat ttggcattag cttatcctat aaggaacaac
     5281 caagcccaga tggtttagca caagctttca ttattggaga ggagtttatc ggtgatgacc
     5341 gtgttgctct tattttagga gataatattt accacggtaa tggcctcact aaaatgcttc
     5401 aaaaggcagc tgccaaagaa aaaggagcca ccgtttttgg ttatcaagtg aaagaccctg
     5461 agcgctttgg agtggtggaa tttgatgaga acatgaatgc tatctctatc gaagaaaaac
     5521 cagaagtacc taagtcccac tttgcagtga ctggacttta tttctatgac aatgacgtgg
     5581 tagagattgc taaaaacatt aaaccaagcg cgcgtggtga gttggaaatt acagacgtca
     5641 acaaggctta cttggatcgt ggtgacctct ccgttgaatt gatggggcgt ggttttgcat
     5701 ggctagacac tggaacgcat gaaagtctac ttgaagcggc tcaatatatt gaaactgttc
     5761 aacgcttgca aaacgctcaa gtggcaaatc tggaagaaat tgcctatcgc atgggttata
     5821 tcagtaaaga agatgtccat aaattggcgc aatctttaaa gaaaaacgaa tacgggcaat
     5881 acttgcttcg tttgattgga gaagcttaat gacagaaact ttttttgaca aaccattggc
     5941 ttgccgagaa atcaaagaaa tcccaggctt actagagttt gatattcctg ttcgtgggga
     6001 taatcgtggc tggtttaaag aaaatttcca aaaagaaaaa atgttgccaa ttggctttcc
     6061 agaacgtttt tttgaggaag gaaaactaca aaataacgtt tctttttcac gtcagcatgt
     6121 gctgcgtgga cttcatgctg aaccttggga taaatacatc tcagttgctg atgatggcaa
     6181 ggttttagga gcatgggttg atcttcgtga gggagagact tttggaaacg tctaccagac
     6241 agtgattgat gcttcaaaag gaatgtttgt tcctagaggg gttgctaatg gctttcaagt
     6301 tctttcagag actgtctctt acagttatct tgtcaatgac tactgggctc ttgacttgaa
     6361 acctaagtat gcttttgtaa actatgctga cccaagtctt gggattactt gggaaaatct
     6421 agcagctgca gaagtttctg aagcagataa aaaccatcct cttcttagtg atgtcaaacc
     6481 actgaaacca aaagacctat agggaaacaa catactcaga tgattgggat taatcatttg
     6541 aggtggatat gagaggagtt attcttctct acttcaggta acaagtaaca actaaaaatg
     6601 atgaaaggaa agttcagttt ctctctttga gctgaacatg ggactcaaat cttagaaagc
     6661 aatgttttct taggcctaaa acgtctttgt tagagtcatt acttttaata gattttttcg
     6721 gaaacgaaag gtctctttat atcttatgta taaaaatatt atcgtaactg gtggagctgg
     6781 tttcatcggt tctaactttg tacactatgt ctacaataac cacccagatg ttcatgtaac
     6841 tgtccttgat aaattgacat atgcaggtaa ccgtgctaac attgaagcta ttcttggtga
     6901 tcgtgttgag ttagttgttg gtgatatcgc tgacgctgaa ttggtagata aattggctgc
     6961 caaagcggat gctattgttc actatgcggc tgagagccac aacgataact cattggaaga
     7021 tccaagtcca tttatccata caaactttat cggaacttac actttgcttg aagcagctcg
     7081 taaatacgat atccgtttcc accacgtgtc aactgatgaa gtttatggag atcttccact
     7141 tcgtgaagac cttccaggac agggcgaagg accaggtgaa aaattcactg ctgaaacaaa
     7201 atacaatcca tcatcacctt actcatcaac taaggcagct tctgacctta tcgttaaggc
     7261 atgggtacgt tccttcggtg tgaaagcgac catttcgaac tgttcaaata actatggacc
     7321 ataccaacac atcgaaaaat tcattccacg tcaaattaca aatatcttgg caggaatcaa
     7381 accaaaactt tatggtgaag gtaaaaacgt ccgtgactgg attcatacta atgatcattc
     7441 tacaggagta tgggctattt tgactaaggg tcgtatcggt gaaacatacc ttattggtgc
     7501 cgacggcgag aaaaacaaca aggaagttct tgaattgatt cttgaaaaaa tgggtcaacc
     7561 aaaagacgct tatgatcacg taactgaccg tgctggtcac gatcttcgtt acgctattga
     7621 ttctacaaaa ttgcgtgaag aactcggctg ggaaccacaa tttacaaact tttcagaagg
     7681 tttggaagaa actattaagt ggtacacaga aaatgagaca tggtggaaag cagaaaaaga
     7741 tgctgtagaa gccaagtatg ctaaaactca agaagtgatt aaataaaaca ttaaagaacc
     7801 ttgtcatatc aacgtttgtt gaagatgtca aggttttttc ttgtgttttg gggcattttt
     7861 ggggcatatt atagccgatt taagatgtcg acgacttcat ttttcatgct tttcgtgacg
     7921 tgggtataga tttgttgagt agttttcgag tcagcatgtc caactctatc catgatagtt
     7981 tttaagggca ctttgttttc tgcaaggcga ctcaccaggg tatgacggaa gatgtggctg
     8041 gtgagttctt tttgaatcgg tttttctagt ctcttatttg ccgctctgat agaggtgttg
     8101 agggcgttgt cttgaatagg aacaccattt ctggaaatga agatgtagcc catgtccttg
     8161 taattaggat tagtattttt ttcaagagca ttaattttca gtatatcctg aataatttct
     8221 ttttcacgtt tggttatcaa tgtttcacgc caactggaat ttgttttagg ggtggttttg
     8281 atagcgtttc tatagccatt tgaggtgtag tcaagagttc cgtggatttc aatgatatca
     8341 tttttgatat tgtctggctg tatagctaga gcttctccaa tgcggcagcc atttaagctc
     8401 ataaattctg ccagataagc cattttaatc gatcctttac gtctatacat ttcatctaat
     8461 agccttttga gttcatcttt ttctaaaaac ttctgggcaa cgtcttcaaa gtctttgatg
     8521 gtcttaacag gttttgcgag ttgggtacta ataactggat tgattgagac atactctaaa
     8581 gcgatagcgt agtcgaaggt ttgtttgagc agctgccgtg ctcgtttacg tttgtcataa
     8641 ctacctggta ttttgtcgat aatactttgc aggtatttcg tggttatctt ggcaatcatc
     8701 acttctggat caattaagtt tcgcaattct cttattctaa aatcaagaga gcggacagaa
     8761 gttgatttga gtgatttctg atgatgttcc caccattcgt tcatgacatc tagtaggtag
     8821 gcatcggttg tggtaagact tgttaagatt ttagatattt tttcgtcaag atatttttgg
     8881 gcttcttttc gtattcgtgg cgtgtctttt tccataagga cagaagctct gcaccacttg
     8941 ccagtatacg ggtgtttgta ccgttctaca aaatttactt ttccgctttt gtgtttttct
     9001 gaccacattg ttttttacct cattttctgt taaaataggt atagtaaaga gacctactgc
     9061 gaagcaggtt tttactatta ccaacttgac ctacacgttt gcttgccggc gctgtgtagg
     9121 tcttttttta atttgctttt tttatattta ttgtattttc tgggactgct aatccattag
     9181 agtagaaaat atactctatt cctttacttg gttttgttac tgaatagttc aggtaatatt
     9241 tttcatactt aaattcttta tacatttgaa aaatttcttc agataaatca taagtgagaa
     9301 tccaattttt atttgacata taatgtttaa ttgtactgga cagactatga tgattttcat
     9361 gattgtaaaa gtttgtatat aatcctggtc cttttgtgta atatggaggg tcaaaaaaag
     9421 tgaaagaatt ttttgttttg gtgatattta aacgaatgaa cttttcagca tccatattat
     9481 aaagtttaat ttgtttttta taagaggcaa ttagctttat ttttttaata gttttttctt
     9541 tgttgaaacg gcaatctaat ttatagttcc catcttgttt taacccacca attacaccag
     9601 cttttatgat acctgagcga tttgttcgat tgaggaataa ggttgagaaa cctaaggtta
     9661 gcaagtcgtc acaattattt ttattttttt ggacttctct ttgaagtttc cactcatcaa
     9721 tggtgatagg agtagtattt attttttcga ttagctgttc cgtataattt aatactgaat
     9781 accaaaaagc atagatagct ttgtcatagt cgttaaccat gattttatga acattgttat
     9841 ttaagagtaa ctttaaggct attcccatcc ctcccatata gggttcaata taggtgattg
     9901 catcattagc tataacgagt tctcttacat aatcataaac ttgagactta ccaccgggat
     9961 atcttaacgg actaatgttt ttcataagct attcctcctt tccatataga atacactatt
    10021 tttttaaagt tggccagtaa taaatggcca acttgaagta ggattgatat tataaaacgg
    10081 tgcggtctta tgaaaagcta tttttaagtt gtcaaaaaat ttcaatacct catcttgatt
    10141 attagtatca tctatccaat atttcataac ataattccaa aaatttgtgt cacaattata
    10201 gatcttttta ttttcttctc tttttacacc gtggtgagaa ccaggttgat ttaattttgc
    10261 ttctagttca tcaagttgag taataaaaga ttcttgaaca agttttttcg tatatcctgc
    10321 tgcgataata tcttgcttct gccaaaaatt gtagtcatct aataagactt ctttaagata
    10381 cttaaaaata agttcttctg gagaataatt ccctggtaga ctaaataaat tgtgtgttaa
    10441 atattgctga cctaattgtt catcaccgtc agtgattgat atgattctta gtgatgactg
    10501 attggtatat ctatcttggg ctagtgcctt tatcgccgaa gaggatattt tagcatctat
    10561 tatgtggaag aatgattcta gtgataattg gtatctttca aaatatgctt gaagtaaatt
    10621 ttgaaaaaaa tctttagctt cgttatcttc taataaaaca ggtataggtt gcttgacata
    10681 tacattgttc actaatttat tttgtaacaa ggcattaatt gagtatttat tgagatgttt
    10741 taataaatca acatgatcta tttggtcaat taaatagatt atattgccta tttttttatc
    10801 tagtgagtaa tcaatcaatg agagactatg agtagtgaaa aaaatctgta ttttataatc
    10861 ttttgaaaaa gtcttaaatt cttctaaaag tttaatttga aattcaggat gcaaagaggc
    10921 atctaattca tcaatgagta gaatactttc tactgttttc tcggtatcgg gagtaatact
    10981 ttcataataa gcttttagtg atacaagtga aatcaatatg gttaaaaggt tgtcttctcc
    11041 agcagatatt gtatttgagt caataccttc ggtgtttgtt gaaaactctg ttttagatct
    11101 gatgcctccc aaattgttta atttaccatc aaatttaatt tcgtatccgg tgaatttttt
    11161 gtaaagcata cttagctgat caagatactg ttttggtaat ttattgataa tattgtgaaa
    11221 cttactttca ccctcccatt ctccgtacga gaatagccgg aacatcccta ggtatattat
    11281 tggaatttcc gggagtctat cagaagttcc tcttttatat tttggtttaa tagcaaaacg
    11341 ctcagtatta ttaacagttc tactattgtg tctcctaaaa tctagtgaat aattttcgta
    11401 aaatgctgtg taaagtgttc cagtcatacc aggagcagga tcattgtatt ttttatctcc
    11461 tctggccaac gattcgattt taggattaaa tattttgttc attttctgaa ttgttgatag
    11521 gattggattt cttgaagaat tcgagactct ttgatgggaa ttactaatta aatgcagaat
    11581 ggatgattta caagttccat tagtaccggc tattatatta atattcttat caaaatcaat
    11641 cgtaatgttt ttaagttttc tatactgtat aaattctatt ttttccatct tacactccca
    11701 cgatattctg aaactcttcc tgtatcattt gttgccccca ggtagtcgag atttgatatg
    11761 tcgtagcaaa ctgtaaccaa ttaaaatcaa cgacattgtg ggatgacaag taatttttga
    11821 tcaattccct aatcataaat ctatcggctt gattttcata ttttagtagc aatctttgat
    11881 attgcttagg atcgtgttcg atatggccca gctcatgtaa aataactttt tctttttcta
    11941 tttgtgatag attttgattg acataaatta tcctaaggtc aggatagtaa aaccctgacc
    12001 ttttccacat tgtgtcggga aactcgaata actttatgtt gtacttacta agcatctcat
    12061 ttatcttcac cattccctac tcctaaagat attttaataa tagcttcaat tttctctacg
    12121 tctttgtcag acaaaggttt gccgtcgaac atcacaacct tttcgcgtag attggatagg
    12181 tcaatagtgg cctgccaatc agttttttct tcagcaaaac tttctgggtt aattccaaga
    12241 atatcacata ttttaaaaat agtttctgct tttgcattca tcacaccgcg ctctaaaatg
    12301 gagcgcattg ttgtgtatgg gatatcattt tcaatagcaa aagccctaac gctattatat
    12361 ctactttcga taatcctctt taaatctttt tcatccattt ttttaatttc cttttttctt
    12421 tttctactat ataataacat acgaaaaatc atatttcaag acaaaaaaat aacaaaaaat
    12481 ttaaaaaaac tgttgactgt atacgaaaaa tcatatataa ttaaatcaag cttaaggaaa
    12541 cgacagcttt ttaaaataaa ataatatacg aaaaatcgta ttggaaagga atgatatgaa
    12601 aaacatcgaa cacattcgaa aagaaaaagg tgtttcattg gttgatattg ccgactgttt
    12661 acggttgaaa tctcaaacag ttcgagaaaa aattaacggt gactctgatt ttaaatttgg
    12721 tgaagcattg aaagttcaac aaactttttt tccagaattt gatatcgtct atctttttaa
    12781 agagcgtaaa gaatcatctg taggatgata tacgaaaatt cgtagaaagg aaattatgaa
    12841 tcatctaatt aacgtaacac taaacgaaaa tcaagaacca gtagtaagtg caagagactt
    12901 acataaagaa ttaaaagtca aaactcgttt tagtgagtgg gtaaaacaaa actttaaaat
    12961 tcttgaagaa ggatatgatt ttacaagtgt agttggaact acggttgtaa ataatggggc
    13021 tgtcagagaa atccaagact acgttctctc acttgatgct gccaaaaatt tggcaatggt
    13081 atccaagact gacaaaggca aagaagtccg taaatacttc atccaggttg aaaaagactt
    13141 caacagtcct gagaaaatca tggcaagggc gttgctgatg gctgataaga aagtgcacaa
    13201 gctggaagca caaatcgagg cagaccgtcc aaaggtgcta tttgctgatg cggttgaggc
    13261 tagtgagaca tctatcttga ttggtgattt tgctaaaatc ttacgtcaga atggttataa
    13321 catcggtcaa aatcgcttat tcgcttggtt gcgtgagaat ggcttcctga ttcgtaaaaa
    13381 tggcgagagc tacaacatgc cgacacaacg aagcatggac atgaagctgt tcgaagtcaa
    13441 ggaacggacg caccaggagc caaatggcag tatccgtatc agcaaaacta ccaagatgac
    13501 aggtaaaggc cagcagtatt ttattaataa atttttaaat gagaaagtgg ggtagagcat
    13561 gagaccaaaa cgatacccat atagcgggaa gaaaaacaga aaagcaaaag acatcagctt
    13621 gagactgatg tctaaagagg aattatctga ttttagtcgt cagattgctg aagccactcg
    13681 tgataatgtc gaaccatttc gagagccgca aagtatgatg agctaagtat taactttgca
    13741 aggtttacag attccccttc gctttctttc gcatatctat tgtaatggta ctcaataact
    13801 tccagaattt cttcggtttt accattgctt agagattggg cgaaattgtt gaaatcttta
    13861 ttcatataat cacctttttt cgagatgatt atatcaaaaa agtccgacgg gaatcggact
    13921 caaaacaa
//
LOCUS       DAUCLR010000048        13852 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.048, whole genome shotgun sequence.
ACCESSION   DAUCLR010000048 DAUCLR010000000
VERSION     DAUCLR010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13852
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.048"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            47..1456
                     /locus_tag="VGT87_001170"
     CDS             47..1456
                     /locus_tag="VGT87_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEQ3814024.1"
                     /translation="MVSVMWFRRDLRIDDQKALAHAIASQSPTLCVFQFNKEQLNLLH
                     SRNQSSFITSVLAFRKLLKCEGIDLYLMYGDLMTCFEQLLTQLKDWTDVYFNYDESGY
                     GRLRDQKAAQFFKKNGIAVHTYQDHYLHGSQEIINQSGQPYKVFTPYYRIWQNYPKET
                     PIKVELSQGRWLNLETPDDVLRTVESFKDEKYQDVATFDEASKQLNRFIQDQLAAYHA
                     NRDFPAQLGTSRLSPFLRIGAIGIRTVYHAVRQAPNSLGQATFLKELAWRDFYNMVYV
                     AYPDQKTQPIQKAFSQIEWVNNPDWFQLWKEGKTGYPIVDAAMLQLQKTGWMHNRLRM
                     IVASFLTKDLLCDWRLGEQYFQQQLIDYDAASNIGGWQWAASTGTDAVPYFRIFNPVT
                     QGKRFDPKGEFIKAYLPQLEHVPEKYLHEPWKMPKYLQESVSCIIGTDYPQPIVDHAK
                     QREQAIAKYEWAKEKAKIE"
     gene            1634..3328
                     /locus_tag="VGT87_001171"
     CDS             1634..3328
                     /locus_tag="VGT87_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEQ3814025.1"
                     /translation="MTYNEVYQEWLHNNDLSDDIKADLAAIKDNEAEIQDRFYKTLEF
                     GTAGLRGKLGAGTNRMNTYMVGKAAQALANTIIDHGPEAVKKGIAVSYDVRYQSRTFA
                     ELTCSIMAANGIKAYLYKGIRPTPMCSYAIRALGCISGVMITASHNPQAYNGYKAYWQ
                     EGSQILDDIADQIAQHMAALTQYEEIKQMPFEKALDSGLVTYIDESIEEAYKKEVLGL
                     TINDTDIDKSVRVVYTPLNGVGNLPVREVLRRRGFENVYVVPEQEMPDPDFTTVGYPN
                     PEVPKTFAYSEKLGKAVDADILIATDPDCDRVALEVKNAVGDYVFLNGNKIGALLSYY
                     IFSQRFDLGNLPANPVLVKSIVTGDLSRAIASHYGIETVETLTGFKNICGKANEYDVT
                     KQKNYLFGYEESIGFCYGTFVRDKDAVSASMMIVEMAAYYKKKGQNLLDVLQTIYATF
                     GYYNERQIALELEGIEGQKRIARIMEDFRQTPIASVAEMALDKTIDFIDGYQDFPKQN
                     CLKFYLDDGSWYALRPSGTEPKIKFYLYTIGQTQENSATKLDAIEAACRTKINQVN"
     gene            3535..4389
                     /locus_tag="VGT87_001172"
     CDS             3535..4389
                     /locus_tag="VGT87_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEQ3814026.1"
                     /translation="MTELIDGKALAQKMQQELAAKVNNLKQKKGIVPGLAVILVGDDP
                     ASQVYVRNKERAALTVGFKSETVRLSEFICQEELIAVIERYNADNTIHGILVQLPLPN
                     HINDKKIILAIDPKKDVDGFHPMNTGHLWSGRPLMVPCTPSGIMELLREYNVNLEGKH
                     AVIIGRSNIVGKPMAQLLLDKNATVTLTHSRTRQLEEVCRCADVLIVAIGQGHFITKQ
                     YIKDGAIVIDVGMNRDDNGKLIGDVALDEVAEVAAKITPVPGGVGPMTIAMLLEQTYQ
                     SALRSTHK"
     gene            4542..5882
                     /locus_tag="VGT87_001173"
     CDS             4542..5882
                     /locus_tag="VGT87_001173"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEQ3814027.1"
                     /translation="MADYLTVTHLTKYLKLKFDRDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDESAVIQATMWAGVYKKLGFDLEEGMKINVIGRVQLYEPSGSYSIVIEKAEPDG
                     IGALALQFEQLKKKLTAEGYFEQKHKQLLPQFVSKIGVITSPSGAVIRDIITTVSRRF
                     PGVEILLFPTKVQGDGAAQEVVANIRRANQREDLDLLIVGRGGGSIEDLWAFNEEIVV
                     QAIFESQLPVISSVGHETDTTLADFVADRRAATPTAAAELATPITKTDLMSWIVERQN
                     RSYQACLRRIKQRQEWVDKLSQSVIFRQPERLYDAYLQKIDRLSMTLMNTMKDRLSSA
                     KENKVQLDHALANSQLQTKIERYQDRVATAKRLLMANMASQYDSQLARFEKAQDALLS
                     LDASRIIARGYAMIEKNQALVASVSQITKGDQLTIKMRDGQLDVEVKDVKNENI"
     gene            5860..6075
                     /locus_tag="VGT87_001174"
     CDS             5860..6075
                     /locus_tag="VGT87_001174"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEQ3814028.1"
                     /translation="MSKTKTFEENLQDLETIVNKLENGDVPLEEAISEFQKGMLLSKE
                     LQKTLQAAEKTLVKVMQADGTEVDMDD"
     gene            6075..6947
                     /locus_tag="VGT87_001175"
     CDS             6075..6947
                     /locus_tag="VGT87_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEQ3814029.1"
                     /translation="MDKLARIDEAIRRYYKTTSNGVSEELIDAILYSVDSGGKRIRPL
                     ILLEMIEGFGVSLQNAHFDLAAALEMIHTGSLIHDDLPAMDNDDYRRGRLTNHKQFGE
                     ATAILAGDSLFLDPFGLIAQAELNSEVKVALIQELSLASGTFGMVGGQMLDMKGENQA
                     LSLPQLSLIHLNKTGKLLTFPFKAAALVTEQAITVRQQLEQAGMLIGHAFQIRDDILD
                     VTASFEDLGKTPKKDLFAEKATYPSLLGLEASYQLLTESLDQALTIFQTLENDVGFKP
                     QIITKLIEGLRLNA"
     gene            6940..7767
                     /locus_tag="VGT87_001176"
     CDS             6940..7767
                     /locus_tag="VGT87_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEQ3814030.1"
                     /translation="MPKERVDVLAYKQGLFETREQAKRGVMAGLVVSVINGQRYDKPG
                     DKIDDGTELKLKGEKLKYVSRGGLKLEKGLHVFGVSVANQIGIDIGASTGGFTDVMLQ
                     DGAKLVYAVDVGTNQLVWKLRQDPRVRSMEQYNFRYAQPEDFSEGQPVFASIDVSFIS
                     LSLILPALHNVLSDQGQVIALIKPQFEAGREQIGKKGIVKDKQIHEKVIQKVMDFASG
                     YGFTVKGLDFSPIQGGHGNIEFLAHLAKSQTPETLAPHLIQKVVAKAHKEFEKHEKE"
     gene            7754..8224
                     /locus_tag="VGT87_001177"
     CDS             7754..8224
                     /locus_tag="VGT87_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEQ3814031.1"
                     /translation="MKKSERLELIKKMVLTHPIETQHDLLRLLAEHGLELTQATISRD
                     MNEIGIVKIPSGSGRYIYGLSQDSGKKIVQGPRSIKSTILAVSDKTKGLEQHLYLKVV
                     PGNSKLIKRYLLADFSKAIFSLIADDDSLLLIAKSPSEADMIRQEILLWMQGIT"
     gene            8246..9907
                     /gene="recN"
                     /locus_tag="VGT87_001178"
     CDS             8246..9907
                     /gene="recN"
                     /locus_tag="VGT87_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEQ3814032.1"
                     /translation="MLLEISIKNFAIIDEISLNFENGMTVLTGETGAGKSIIIDAMNM
                     MLGARASTEVIRRGANKAEIEGFFSVDATPELVACLESSGIAMEEELIIRRDIFANGR
                     SVSRINGQMVNLATLKQVGQFLVDIHGQHDQEELMRPQLHQQILDAFGDKAFEQLKEN
                     YQLIFDRYKSLRRQVIDKQKNEKEHKDRIDMLAFQIAEIEAAALSRGEDDRLNQERDR
                     LMNHKQIADTLTNAYVMLDNDDFSSLSNIRSSMNDLLSIEQFDSEYKGMSTSISEAYY
                     ILEEVSKQLSDTIDQLDFDGGRLQEIEFRLDTLNSLTRKYGGNVNDVLDYYDNIIKEY
                     QLLTGDDLSSGDLEAELKSLEKQLVAAASELSVSRHQLAEQLEAEIKAELKELYMEKA
                     DFKVHFTTSKFNRDGNESLEFYISTNPGEGFKPLVKVASGGELSRLMLAIKAAISRKE
                     DKTSIVFDEVDTGVSGRVAQAIAQKIYKIGRHGQVLAISHLPQVIAIADYQYFISKES
                     KEESTVSKVRLLTPEERVEEIASMIAGTDMTQAALTQARELLAKH"
     gene            10079..11008
                     /locus_tag="VGT87_001179"
     CDS             10079..11008
                     /locus_tag="VGT87_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEQ3814033.1"
                     /translation="MTSKKACLSSIIVLASLTCGNDTVSANHLSATGDKFDDCSTLVE
                     KDVAPKDELEMLAWSSSQTTDDADRDYEDFLDDDSFNDDSFISQNETDKMFENLTDDR
                     LLDELDEEDTIEPEQNVIMPSDDELFDLTDAVETRLTVSSAPHLEAELPKPHLRSLSD
                     TALRSGEIRGHLDNKLDALSVTATKLALTMAQKFDLTTHVYSIGESFSEVLAAHYEDR
                     KAESAFSKKKRFHLPIATPDVVIEELRRLVSSIGSSKEDVSVPYNRKLGMAVAKRKIA
                     LPQTGEEFSYYPALLGLMILGLTPIMIPKKINN"
     gene            11224..12075
                     /locus_tag="VGT87_001180"
     CDS             11224..12075
                     /locus_tag="VGT87_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEQ3814034.1"
                     /translation="MEKYMGTIKIVTDSSITIEPELIKALDITVVPLSVMIDSKLYSD
                     NDLKEEGHFLSLMKASKSLPKTSQPPVGLFAETYENLVKKGVTDIVAIHLSPALSGTI
                     EASRQGAEIAEAPVTVLDSGFTDQAMKFQVVEAAKMAKAGASLNEILTAVQAIKSKTE
                     LYIGVSTLENLVKGGRIGRVTGVLSSLLNVKVVMALKNDELKTLVKGRGNKTFTKWLD
                     SYLAKNSHRPIAEIAISYAGEASLALTLKERIAAYYNHSISVLETGSIIQTHTGEGAF
                     AVMVRYE"
     gene            12068..12910
                     /locus_tag="VGT87_001181"
     CDS             12068..12910
                     /locus_tag="VGT87_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEQ3814035.1"
                     /translation="MNNRHLFSGIFFFVISLCLAFLLLNIIIPKSNSRLKKSDFLKKE
                     QVAIQYVAIGDSLTEGVGDLTHQGGFVPLLTNDLSEYFKANVNHQNYGVSGDTSQQIL
                     DRMIKQKQIQLSLKKADIMTLTVGGNDVMAVIRKNLADLQVSSFRKPARQYQKRLRQI
                     IELARKDNKDLPIFILGIYNPFYLNFPELTDMQKVIDDWNIKTKEVVGEYDRVYFVPI
                     NDLLYKGINGQEGIVHSSGDQTTIVNDALFTGDHFHPNNTGYQIMSNAVMEKIKKHEK
                     KIKP"
     gene            12888..13475
                     /locus_tag="VGT87_001182"
     CDS             12888..13475
                     /locus_tag="VGT87_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEQ3814036.1"
                     /translation="MKKKSNLNWWKWSFLCLLAFNTAFLMVIASRLIQVREPESELIA
                     KKPVKNIKIGTFVTTREQLNETVASYLKDYQTKKMSYKFYATSSSILFEGTYQLLGYE
                     VPLYIYFQPHRLENGAVQLQVISFSVGTLPLPEKDVLQYLKSSYKLPSFVKVMPNQSA
                     IVVNLQDIQNDAKVYLKAKKIDLFNDEISFNIYKK"
     gene            13574..13849
                     /locus_tag="VGT87_001183"
     CDS             13574..13849
                     /locus_tag="VGT87_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEQ3814037.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVDAVFSTIEAFLAEGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGAEIEIAASKVPAFKAGKALKDAVK"
BASE COUNT         4491 a         2295 c         2953 g         4113 t
ORIGIN      
        1 taaaaagaaa tagttgcatt atttgcatag aagaaaggga tctgttatgg tttcagtgat
       61 gtggtttcgt agagatttaa ggattgatga tcaaaaagct ttagctcatg ctattgctag
      121 tcaatcacca accttatgtg tgtttcaatt taacaaggag caattaaatc tgctacatag
      181 tcggaatcaa tctagtttta ttactagcgt tttggctttt agaaagttat taaaatgtga
      241 aggtattgac ttatatctaa tgtatggaga cttaatgacc tgttttgagc aattattgac
      301 acaattaaaa gactggacag atgtgtattt taactatgat gaatctggat atggtcgttt
      361 acgtgatcaa aaagcagcac aatttttcaa aaaaaatggg attgctgttc atacctacca
      421 agatcactat ttacatggta gtcaagagat cattaaccaa tcaggtcagc cttataaagt
      481 ttttacgccc tactatagga tttggcaaaa ttatccgaaa gagactccta ttaaagtaga
      541 actatcgcaa ggcaggtggt taaatcttga gacaccagat gatgttttaa ggactgttga
      601 gagttttaaa gatgaaaaat atcaggatgt tgcaactttt gatgaagcgt caaaacaact
      661 aaatcgattt attcaagacc aattagcagc ttatcacgct aatcgagatt ttccagcgca
      721 gctagggacg agtcggttgt ctcctttttt aagaatagga gctattggca ttcgcacggt
      781 atatcacgct gtgcgtcaag caccaaatag tctagggcaa gctacttttt taaaagaatt
      841 agcttggaga gacttttaca atatggtcta tgtggcttat cctgatcaga aaactcagcc
      901 tatccaaaaa gccttttctc aaatcgaatg ggttaataat cctgactggt ttcagttgtg
      961 gaaagaaggt aaaacgggtt accctattgt tgatgctgcc atgcttcagt tacaaaagac
     1021 aggatggatg cataatcgtt tacgaatgat agtagcttct tttttaacta aagatctttt
     1081 atgtgattgg cgtttaggag agcagtattt tcaacaacaa ctaattgatt atgatgctgc
     1141 cagtaatatc ggaggatggc agtgggctgc ttccactgga acagatgctg ttccttattt
     1201 tagaatattt aatcctgtta ctcaaggtaa gcgttttgat cccaaaggcg agtttattaa
     1261 agcgtatttg cctcaactag aacacgttcc agagaagtat ctccacgaac cgtggaagat
     1321 gccaaaatac cttcaagaga gtgtatcctg tattattggc acagactatc ctcaaccaat
     1381 tgtcgatcat gctaaacaac gtgaacaagc tattgcaaag tatgagtggg caaaagaaaa
     1441 agcaaaaata gaatgactgt tctcattata agagtttaaa caaaaagtta tcatgaatta
     1501 agattattta atttaaaaaa taagttgact ttatttagat tatttgatac tattcagttg
     1561 tctcccttag agaaagcgat ttcttgtttt ggagctgatt tttaccattt ggtcttagaa
     1621 taggatgagt aatatgactt acaacgaggt atatcaagaa tggttgcaca ataatgatct
     1681 tagtgatgat attaaagcag atttagcagc cataaaagac aatgaggctg agattcaaga
     1741 tcgtttttac aaaacacttg aatttggaac agcagggcta agaggaaaac ttggagcagg
     1801 caccaatcgc atgaatacct atatggttgg taaggcagca caagctttag ctaatactat
     1861 tattgatcat ggacctgaag cggttaaaaa gggcattgcg gttagttatg atgttcgcta
     1921 tcaatctaga acatttgcag aattaacatg ctctattatg gcagctaacg gtattaaggc
     1981 gtacctttat aaagggattc ggccaacacc aatgtgttcg tacgctattc gtgctttggg
     2041 atgtatctct ggtgttatga ttacagctag tcacaatccc caagcttaca atggttataa
     2101 agcttattgg caagaaggtt ctcaaatttt ggatgacatt gcggatcaaa ttgcacaaca
     2161 catggctgca ctaactcagt atgaggaaat caaacaaatg ccttttgaaa aggctctgga
     2221 cagtggactt gttacttata ttgatgagag tattgaagaa gcatataaaa aagaagtgct
     2281 tggtttaacg attaatgata ctgacattga taagtcagtc cgagtagtct ataccccttt
     2341 aaatggtgtt gggaatctac cagtgcgtga ggttttaaga cgccgcggtt ttgaaaatgt
     2401 ttatgtggta cctgagcagg aaatgcccga tcctgatttt acaacagttg gctatcctaa
     2461 tcccgaagtc cctaaaactt ttgcatattc agaaaaatta ggtaaggcag ttgatgctga
     2521 tattttgatt gcaactgatc cagattgtga ccgtgtggct ttagaagtta aaaatgcagt
     2581 cggtgactat gtttttctca atggcaataa aatcggagcg cttttatctt actatatctt
     2641 ttcgcaacga tttgacttag gcaatttacc ggctaatcct gttttagtga aatccattgt
     2701 aacaggagac ttgtcacggg ctattgctag ccattatggt attgaaaccg ttgaaacatt
     2761 gactggtttt aagaatattt gtggcaaggc aaacgaatat gatgtgacga aacagaaaaa
     2821 ctacctgttt ggttatgaag aaagtattgg tttttgctat ggcacttttg ttcgtgacaa
     2881 agacgctgtt agcgcctcta tgatgattgt agaaatggca gcttactaca agaaaaaggg
     2941 gcagaattta ctagatgttt tgcagactat ttatgcgaca tttgggtatt ataatgaacg
     3001 tcaaattgcc ttggagcttg aggggataga aggacaaaaa cgcattgcga ggataatgga
     3061 agatttcaga cagacaccaa tagctagtgt tgcagagatg gcgttagaca agacaattga
     3121 ttttattgat ggctatcaag attttccaaa gcaaaactgt ctcaaatttt atttggatga
     3181 tggttcttgg tatgcactta gaccatcagg aacagagcct aaaataaaat tttatttata
     3241 tactattggc caaacacaag aaaatagtgc aacaaaatta gatgctattg aagcagcgtg
     3301 tcgcacaaaa ataaatcagg ttaattaaac atcaaaaatg gataacactt ttacactatt
     3361 ttccatataa acaactacaa aaggaggtga gacttttagg caccttcttt ttcttgtagg
     3421 tgccactaat caatttaatc gactcaaacc aatactaaaa aataagtaag agtataaaga
     3481 acaggcatct atttttttgt tataatagaa aaatgaaatt aaagggagat gtcaatgaca
     3541 gaactaattg atggtaaagc cttagctcaa aagatgcaac aagagttagc agctaaagtc
     3601 aacaatctaa aacaaaaaaa aggaattgta ccaggcttag ccgttattct tgtaggtgat
     3661 gatcctgcta gtcaggtgta tgtccgtaat aaagagcgtg cagctcttac tgtaggtttt
     3721 aaaagtgaga ccgttagatt atcagaattc atttgtcaag aagagcttat tgcagtaatc
     3781 gaacgttaca atgcagataa cactattcat ggtattttag tgcagctacc cctgccaaat
     3841 catattaatg ataaaaaaat tattctcgcc attgatccca aaaaagatgt ggatggtttt
     3901 cacccgatga atacaggtca cctttggtca ggacgtccct taatggttcc ttgtactcca
     3961 tcagggatta tggaattgct tcgagaatat aatgttaacc ttgaaggtaa acatgccgtc
     4021 attattggca gatcgaatat cgttggaaaa ccaatggcac agctcttact agacaaaaat
     4081 gcaacagtca cgttgacaca ttcaagaaca cgtcaattag aagaagtatg tcgctgtgca
     4141 gatgtgttga ttgtggcaat cggacaaggt catttcataa caaaacaata tataaaagat
     4201 ggtgcgatag tgattgatgt gggaatgaac cgtgatgata atggcaagct aattggagat
     4261 gtggccttgg atgaggtggc agaagttgca gcgaaaatca cccctgtacc aggaggtgtc
     4321 ggtcctatga cgattgctat gttgctagag caaacttatc aatctgctct ccgtagtact
     4381 cataaatagt tatcattaga caattcaaca taagagaata taagaagtca gcaatggctt
     4441 ccttttttgt ttttctgctc actatttatc cagaaaaatg gtataattgc attagtatgc
     4501 aaagggacta tgtcaggtcc aactaagtga aaggtggctt gatggcagat tatttaaccg
     4561 tcactcattt gacgaaatat ttaaaattaa aatttgaccg tgacccttat ttggaacggg
     4621 tttatctgac tggtcaagtg tccaattttc gaaaacgacc aactcatcaa tatttttcct
     4681 taaaagatga aagtgctgtg attcaagcca ccatgtgggc aggagtctat aaaaaactag
     4741 gatttgacct agaagaaggc atgaaaatta atgtgattgg gcgagtccaa ctttatgaac
     4801 cgagtggttc ctattctatc gtgattgaaa aggcagagcc agacggcata ggtgccttgg
     4861 ctttgcagtt tgaacaatta aagaaaaaat taactgcaga aggttatttt gagcaaaaac
     4921 acaagcagct cttaccacag tttgtgtcaa aaattggggt cattacaagt ccaagtggcg
     4981 ctgtgattcg agatattatc acaactgttt cacggcgttt tccaggggtt gagattttat
     5041 tattcccaac taaagtacaa ggtgatggag ccgcccaaga agtagtggcg aatattcgaa
     5101 gagccaacca aagagaggat ttggacttgc tcattgttgg ccgtggaggt ggctcgatag
     5161 aagacctttg ggctttcaac gaggaaatag tagttcaggc tatctttgaa tcgcaacttc
     5221 cagtgatttc gagtgtgggt catgaaactg atacaaccct ggcagatttt gtggcagatc
     5281 gcagagcagc cacaccaaca gcagcagcag agttagcaac gcctattaca aaaacagatc
     5341 ttatgtcttg gatagtagag cggcaaaatc gttcttatca ggcctgcttg aggcggatta
     5401 agcaacggca agagtgggtg gataaactat cacaatctgt tatttttagg caaccagaac
     5461 gattatatga tgcctacctg caaaaaattg atcgccttag catgaccctg atgaatacga
     5521 tgaaagatcg tctgagctcc gccaaagaaa acaaggttca gttggaccat gccttggcca
     5581 atagtcaatt acagacaaaa atcgagcgat accaagaccg tgttgctacc gctaagcgtt
     5641 tactgatggc taatatggct agtcaatatg atagtcagct ggctcgcttt gaaaaagcac
     5701 aggatgcttt gctatcactg gatgccagcc gaattatcgc ccgtggttat gcaatgattg
     5761 aaaaaaatca agcactggta gcctctgtaa gtcagataac aaagggtgat cagttaacca
     5821 ttaagatgcg tgatggacaa ttagatgtag aggtaaaaga tgtcaaaaac gaaaacattt
     5881 gaagaaaatt tacaagattt agagactatt gtgaacaaac ttgaaaatgg ggatgtccct
     5941 ttggaagaag ctatttcaga atttcaaaaa ggaatgcttc tctcaaaaga acttcaaaag
     6001 accttacaag cggcagaaaa aacactcgtc aaagtgatgc aagctgatgg cacagaagta
     6061 gatatggatg attaatggac aaattagcta gaattgacga agccattcgt cgctactata
     6121 agacgactag taacggtgta tctgaagagt tgattgatgc tatcttgtat tctgttgaca
     6181 gtggtggcaa gcgcattcga ccacttatct tattagagat gattgaggga tttggggtat
     6241 cgttacagaa tgctcatttt gatttggctg cagcccttga aatgattcat acaggaagtc
     6301 tgattcacga tgatttgcca gccatggata atgatgatta ccgacgtggt agactgacca
     6361 atcacaaaca atttggagaa gccacggcca ttttggcggg agatagctta tttttagacc
     6421 cctttgggtt aatagctcaa gctgaattaa atagcgaggt taaagtagcc ttaatccaag
     6481 aactttccct agcttcagga acttttggca tggttggtgg tcagatgcta gatatgaaag
     6541 gggaaaatca agctttgagt ctccctcagt tgtcactgat tcacctcaat aaaaccggaa
     6601 aattattaac ttttcctttt aaagcagcag ctcttgtaac agaacaggct attactgttc
     6661 gtcaacaact agagcaggcg ggaatgctca tcgggcacgc ctttcagatt agggatgata
     6721 ttttagacgt gacagctagc tttgaagatc ttggaaaaac gcctaaaaag gatttatttg
     6781 cagaaaaagc tacttaccca agtttactgg ggttagaagc ctcctaccaa ctgttgacag
     6841 agagtttaga tcaggctttg acgatttttc agacactaga aaacgatgta ggctttaagc
     6901 ctcaaataat tacaaaactg atagaagggt tacgacttaa tgcctaaaga aagagtagat
     6961 gtgttagcct ataagcaagg attatttgag accagagagc aagctaagcg tggtgttatg
     7021 gcaggcctag tggtctctgt gattaatggc caacgctatg acaagccagg tgacaaaatt
     7081 gacgatggga ctgagttaaa acttaaaggt gaaaaactca aatacgtcag tcgtggtgga
     7141 ttaaaactgg aaaaggggct gcacgttttt ggtgtatcgg ttgctaatca aattgggatt
     7201 gacattggcg cttcaacagg tggttttacc gatgttatgt tacaagatgg ggccaaacta
     7261 gtctatgccg tcgacgttgg gactaaccag ttggtatgga aactcagaca agatccacga
     7321 gtaagaagta tggaacagta taactttcgt tatgctcagc cagaggactt tagtgaggga
     7381 cagcctgtat ttgccagtat tgatgtgtcc tttatttctc tcagcttgat tttgccagcc
     7441 ctgcataacg ttttgtcaga tcagggacag gttattgctc tcattaaacc gcaatttgaa
     7501 gctgggcgcg agcagattgg taaaaaaggc atcgtcaaag acaaacagat tcatgaaaaa
     7561 gtgattcaaa aggtcatgga ttttgcctca ggttacggat ttacagttaa gggacttgat
     7621 ttttccccta ttcagggtgg tcatggcaat atcgaatttt tagctcatct tgctaagtca
     7681 cagacacctg aaacgttagc cccgcatttg attcagaaag ttgttgccaa ggcacataag
     7741 gagtttgaga aacatgaaaa agagtgaacg acttgaatta atcaaaaaaa tggttttgac
     7801 gcatccaatt gagacgcaac atgacctctt aagattattg gcagagcatg ggttagaatt
     7861 aacccaggct actatttcaa gagacatgaa tgaaattggt atagtaaaga ttccttctgg
     7921 cagtggacgt tacatctatg gtctttctca agatagtgga aaaaagatcg ttcaaggacc
     7981 tagatcgata aagagcacta ttttagctgt atctgacaaa acaaaaggtt tagaacaaca
     8041 cctctattta aaagtcgtac ctggcaatag taaattaatc aaacgttatt tattagcaga
     8101 tttttcaaaa gctattttta gtcttattgc tgatgatgat agtttattgt taattgcaaa
     8161 gtctccttca gaagcagata tgatacgcca agaaattttg ctttggatgc agggtataac
     8221 ctagaaaaaa ataggggaga aagtgatgtt attagaaatt tctattaaaa attttgctat
     8281 tattgatgaa atttccctaa attttgaaaa tggtatgacg gttttgacgg gtgaaactgg
     8341 agcaggaaag tctattatca ttgatgctat gaatatgatg cttggtgcgc gtgctagtac
     8401 agaagtgatt cgtcgtggag ctaataaagc agaaattgag gggttctttt cggtagatgc
     8461 taccccagaa ctggttgcgt gtctggaatc atcaggtatt gctatggaag aagaactgat
     8521 tattcgccga gatatctttg ccaatggcag aagcgtgagc cgcattaatg gtcagatggt
     8581 taacctagcc accttaaaac aggtcggaca gtttttggta gatattcatg ggcaacatga
     8641 ccaagaagaa ttaatgcgac cacagctcca ccagcaaatt ttggacgctt ttggtgataa
     8701 ggcttttgag caattgaaag agaactatca gcttattttt gatcgttata aaagcttgcg
     8761 tcgccaggtt attgacaaac aaaaaaatga aaaagaacat aaagatcgta tcgacatgtt
     8821 ggcttttcag atagcggaga ttgaagctgc tgccttgagt cgaggtgagg acgaccggtt
     8881 aaatcaagaa cgtgatcgct taatgaacca caaacaaatt gctgataccc tgaccaatgc
     8941 ctacgtcatg ctagataatg acgatttttc aagtctatcc aatattcgct ctagcatgaa
     9001 tgacttacta tcaattgagc agtttgattc agagtacaaa gggatgtcga cttcgatttc
     9061 tgaagcctat tatattcttg aagaagtgag caagcaatta tcagatacca ttgaccaact
     9121 ggattttgat ggtgggcgat tacaggaaat tgaatttcgc ttggatacac tcaatagctt
     9181 aacccgtaaa tacggtggta atgtgaatga tgtgcttgac tactacgata atattatcaa
     9241 agaataccag ctattaacag gagatgactt gtcttcaggg gatctagagg cagaattgaa
     9301 aagcttggaa aaacaattgg ttgctgctgc ttcggagtta agtgtctctc gccatcaatt
     9361 ggcggagcaa ttagaagctg agattaaagc tgaacttaag gaactttata tggaaaaagc
     9421 agatttcaaa gttcacttta ccacatcaaa attcaatcgt gatggtaatg aaagcttgga
     9481 attttacatc tctactaatc caggcgaagg attcaagccc cttgtcaagg tggcttctgg
     9541 aggagagtta tcacgcctta tgttagccat caaggctgct atttctagaa aagaagataa
     9601 gacgagtatt gtttttgatg aagtagatac aggtgtttct ggtcgcgttg cgcaagccat
     9661 tgcccaaaaa atttataaaa ttgggcgaca tgggcaagtt ctcgctattt cacacttacc
     9721 tcaagttatt gctattgctg attatcagta ttttatttct aaagaaagta aagaagagtc
     9781 caccgtttct aaggtaaggt tattaactcc tgaagaacgt gtggaagaaa ttgctagcat
     9841 gattgcagga acagatatga cacaagctgc tcttacgcag gctcgtgaac tacttgccaa
     9901 acattaagat tatgtttggt atttgatatt ttcataatta gatgacattg attttttagg
     9961 tgctaaaata atcattgtgt cagaccattt ttcctctgtt aatctggctt ggtttgcctt
    10021 gacaaacctc tgagaaatag tagaatagat aagagaattt taagctgagg ggatagaaat
    10081 gactagtaaa aaagcgtgtt tatcaagcat cattgtgtta gcaagtttaa cgtgtggaaa
    10141 tgatactgtt agtgccaatc atctctcagc aactggagat aagtttgatg attgctcaac
    10201 acttgttgaa aaagatgtgg cccctaaaga tgaacttgag atgttagcat ggtcctcgtc
    10261 tcaaacaact gatgatgctg acagagacta tgaagatttt ctcgatgatg attcttttaa
    10321 tgatgattct tttatttctc aaaatgaaac tgataagatg tttgagaatt taactgatga
    10381 taggttatta gatgaattag atgaagaaga tacaattgag ccagagcaaa atgtaataat
    10441 gcctagtgac gatgagctat ttgatttaac tgatgctgtt gagacacgcc ttactgtttc
    10501 tagtgctccc catttagagg ctgaattgcc gaaaccacat ttgaggagcc tatcagatac
    10561 agcactgcgg tctggtgaaa ttagaggaca tttagataac aaactggacg ctttgtctgt
    10621 aacagctaca aagttagcat taacgatggc tcaaaaattt gatttgacaa cgcatgtcta
    10681 ttctataggt gaaagcttta gtgaagtatt agctgctcat tatgaagaca gaaaagcaga
    10741 atcagctttt tctaagaaaa agagatttca ccttcctatt gctactccag atgttgttat
    10801 agaggagtta aggcgcctgg tctcttctat tggaagttca aaagaagatg tttcagttcc
    10861 ttataatcgg aagctaggta tggcagttgc aaaaagaaaa atagccctgc cacaaacggg
    10921 agaggagttc tcttattatc cagctttact tggtttaatg atattaggat taacgccgat
    10981 tatgatacca aagaagataa ataattaggt aataagttat tgtagttgta aaagacgggg
    11041 caaatgttca agcgtgaaaa ggttgacttt tgcctcgttt tgtatctcgt tattgctaag
    11101 gcattttctt cgagcagcta gtgctcgcca tacctatcta ccgagtgact attcaatagc
    11161 actaccagtt ttgctttatt tagcaaggag tgttagaata aagaggtgag aaactagttt
    11221 agaatggaaa aatacatggg aactataaaa attgttacag attcatcaat aactattgaa
    11281 ccagaattaa ttaaagcttt agatattact gtagtgcctt tatctgtcat gattgatagc
    11341 aaattatatt ctgataatga tttaaaagaa gaaggtcatt ttttgagtct tatgaaggct
    11401 agcaaatccc ttcccaaaac cagtcagcca ccggttggtc tctttgctga aacttacgaa
    11461 aatttggtca aaaaaggagt aactgacatt gttgctattc acctctcacc agctttatca
    11521 ggtactattg aagcttcccg tcagggggct gaaatcgctg aggcccctgt tacagtttta
    11581 gattcaggat ttactgatca agccatgaaa tttcaggtcg ttgaggcagc aaagatggca
    11641 aaagcgggtg ctagtttgaa tgagattttg actgctgttc aagctatcaa atcaaaaaca
    11701 gagctctaca ttggtgtatc taccttagaa aatttagtaa aaggaggtcg tattggtcgt
    11761 gtgacgggtg ttttgagctc gctacttaat gtcaaagttg ttatggcact taaaaatgat
    11821 gagttaaaaa cgcttgtcaa aggtcgaggt aataaaacat ttacgaaatg gttagatagt
    11881 tacctcgcta aaaatagtca tcgcccgatt gcagagatag ctatttctta tgctggtgaa
    11941 gctagcctgg cattaacctt aaaagaaaga atcgcagctt attacaacca ctctatttcg
    12001 gttttagaaa caggctctat catccaaaca catacaggag aaggagcttt tgcggttatg
    12061 gttcgttatg aataatcgtc atttatttag tgggatattt ttctttgtta ttagtttatg
    12121 tctggccttt ttattgctaa atattattat ccctaagtca aattcacgtt tgaaaaagag
    12181 tgattttctg aaaaaagaac aagtagctat ccaatatgtt gctataggag attcattgac
    12241 agaaggagta ggtgatctaa ctcatcaagg tggttttgtt cctttgttaa cgaatgatct
    12301 cagtgaatat tttaaggcta atgttaatca tcaaaattac ggcgtatctg gtgataccag
    12361 tcaacagatt cttgatagga tgataaaaca aaagcagata cagttatctt taaaaaaagc
    12421 agatataatg acgttaaccg ttggtggtaa tgatgttatg gcagttattc ggaagaattt
    12481 agcggatttg caagtttcta gttttagaaa gccagctcgt cagtatcaaa aacgattaag
    12541 acagattatc gagttagcca gaaaagataa taaagatctt cctattttta ttttaggcat
    12601 ctataatccg ttttatttga attttccaga actaactgat atgcaaaaag tgattgatga
    12661 ctggaatatc aaaactaagg aggttgttgg agaatacgat cgtgtgtact ttgtgccaat
    12721 aaatgacctc ttgtataaag ggataaatgg acaagaagga attgtccatt cttcaggaga
    12781 tcaaactaca attgtcaatg atgccttgtt tactggggac cattttcacc caaataatac
    12841 tggctatcaa atcatgtcaa atgcagtaat ggagaaaatt aaaaagcatg aaaaaaaaat
    12901 caaaccttaa ttggtggaag tggagcttct tatgtctgct tgcttttaat accgcctttt
    12961 tgatggttat tgcaagtcgt ctcattcaag taagagaacc tgagtcagaa ctgattgcta
    13021 aaaaaccagt taaaaatatc aaaattggta catttgtcac aaccagagaa cagcttaacg
    13081 aaactgtagc aagttatctt aaagattatc agactaaaaa aatgtcttat aaattttatg
    13141 cgacatcttc ttctattttg tttgaaggaa cttatcaatt attagggtat gaagtccctt
    13201 tatatatcta ttttcaacct catcgtctag aaaatggagc tgtccaacta caggtgatct
    13261 ctttttcagt gggaactctg ccactcccag aaaaagatgt tttgcagtat ttgaaatcaa
    13321 gctacaaatt gccaagcttt gttaaagtga tgccaaatca atcagctatc gttgttaatt
    13381 tacaagatat ccaaaatgat gctaaggttt atttaaaagc caagaaaatt gatttattta
    13441 atgatgaaat cagttttaac atctataaga aataagctca aataacgtta aatacttgac
    13501 ctaatcgctt tttttagata tgataatatc tgttaaagca ttaagcttac taattatttg
    13561 gaggatttgt taaatggcta acaaacaaga tttaatcgca aaggttgcag aagcaactga
    13621 attgactaaa aaagattcag cagcagcagt tgatgccgtg ttttctacaa tcgaagcttt
    13681 ccttgctgaa ggtgaaaaag tacaattgat cggttttggt aacttcgaag tacgcgaacg
    13741 tgcagctcgt aaaggtcgta acccacaaac tggtgcagaa attgaaattg cagcttcaaa
    13801 agttccagcc ttcaaagctg gtaaagctct taaagacgct gttaaataat tg
//
LOCUS       DAUCLR010000049        13624 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.049, whole genome shotgun sequence.
ACCESSION   DAUCLR010000049 DAUCLR010000000
VERSION     DAUCLR010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13624
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.049"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(31..1197)
                     /locus_tag="VGT87_001184"
     CDS             complement(31..1197)
                     /locus_tag="VGT87_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEQ3814038.1"
                     /translation="MKNQLGQLALEQAKTFGGKLEVQPKVDIKTKHDLSIAYTPGVAG
                     VSSAIAKDKTLAYDLTTKKNTVAVISDGTAVLGLGDIGPEAAMPVMEGKAALFKAFAG
                     VDAIPIVLDTKDTEEIISIVKALAPTFGGINLEDISAPRCFEIEQRLIKECHIPVFHD
                     DQHGTAIVVLAAIFNSLKLLQKSLDEVSIVVNGGGSAGLSITRKLLAAGATKVTVVDK
                     FGIINEQEAAQLAPHHLDIAKVTNREFKSGTLEDALEGADIFIGVSAPGVLKAEWISK
                     MAARPVIFAMANPIPEIYPDEALEAGAYIVGTGRSDFPNQINNVLAFPGIFRGALDAR
                     AKTITVEMQIAAAKGIASLVPDDALSTTNIIPDAFKEGVAEIVAKSVRSVVLKS"
     gene            complement(1229..2560)
                     /locus_tag="VGT87_001185"
     CDS             complement(1229..2560)
                     /locus_tag="VGT87_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEQ3814039.1"
                     /translation="METISKKMPQKDLSEHSKAWQNRRIGSVPLTVYLVLATLILVTG
                     WLQQLPVNMLGGFAVILTLGWLLGTIGATIPGLKHFGGPAILSLLVPSILVFFNLLNP
                     NVLEATNVLMKQANFLYFYIACLVCGSILGMNRKILIQGLFRMIIPMLLGMVCAMGVG
                     TLVGVILGLDWQHTLFYVVTPVLAGGIGEGILPLSLGYSAITGVGSEQLVAQLIPATI
                     IGNFFAILCTALLNRFGEKHTSYSGQGQLVKIGHSEDMSDALKDNSGALDVKLMGAGV
                     LTACSLFIAGGLLQHLTGFPGPVLMIILAAFLKYLNVIPQETQNGAKQLYKFISSNFT
                     FPLMAGLGLLYIPLKEVVATLSWQYFIVVISVVLTVVSVGFFVSRFLNMNPVEAAIIS
                     ACQSGMGGTGDVAILSTADRMNLMPFAQVATRLGGAITVITMTAILRIIFS"
     gene            2721..4262
                     /locus_tag="VGT87_001186"
     CDS             2721..4262
                     /locus_tag="VGT87_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEQ3814040.1"
                     /translation="MKKPLRLWASLSLILVSMIVVTTSLFYGIMLHDTHQSIKNQETH
                     LLTSTGKMLASHQAIKELLLNNQPNAKTTAYTNSIASIYNLDYVVVMNMKGIRLTHPN
                     PKNIEKPFQGGDEEAVLAGKKVISTAKGTLGKSLRYLVPVFDGDKQIGAIAVGIKLTT
                     LNDVALTSKRNYTLSLLLCLLISLLVTSFISFRLKRQLHQLEPSEIYQLFEERNAMLD
                     QIEAAVFVVDKAGILQLCNQAGQKLIARKCQLGKPTGNSFNHLFPDFPKLSLQEGHEQ
                     LFRYGEEDYLLAISPICVKNDHRGHIIFMREAVKAIDTLDQLAYTTAYASALQAQTHK
                     FMNQLHVIYGLVDIAYYDQLKIYLDSILEPENEILTSLSVLVKEPLLASFLIGEQEKY
                     QELNVHLKIDVLSEIPHSATKNQLNNGLMIYRFIHTNLLTTLRPKSLVLSIQHDQNHL
                     ISHYTLTDNWIDLERVQPIFDLPYFQQLLTDTNSKFHQHQTVTELQFSVTTPYVGGQH
                     ERFNH"
     gene            4243..4908
                     /locus_tag="VGT87_001187"
     CDS             4243..4908
                     /locus_tag="VGT87_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEQ3814041.1"
                     /translation="MNVLIIEDDPMVDFIHRNYLEKLNLFDRIISSDSMKAVQSILTD
                     YAIDLILLDIHITDGNGIQFLEKWRAQHIPCEVIIISAANDGNIIRDGFHLGIIDYLI
                     KPFTFERFQESIQQFVTHREHLANQQLEQAQIDQLKCLTSKKDTKNKQLLEKGLSEST
                     FQWIMENIKAFDQPFTIQELASACHLSHVSVRKYIAYLEENKQLNSQQIFTKVGRPYR
                     VYW"
     gene            complement(4963..6036)
                     /locus_tag="VGT87_001188"
     CDS             complement(4963..6036)
                     /locus_tag="VGT87_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEQ3814042.1"
                     /translation="MRKLYSFLAGVLGVIVILTSLSFILQKKSGSGSQSDKLVIYNWG
                     DYIDPALLKKFTKETGIEVQYETFDSNEAMYTKIKQGGTTYDIAVPSDYTIDKMIKEN
                     LLNKLDKSKLVGMDNIGKEFLGKSFDPQNDYSLPYFWGTVGIVYNDQLVDKAPMHWED
                     LWRPEYKNSIMLIDGAREMLGVGLTTFGYSVNSKNLEQLQAAERKLQQLTPNVKAIVA
                     DEMKGYMIQGDAAIGITFSGEASEMLDSNEHLHYIVPSEGSNLWFDNLVLPKTMKHEK
                     EAYAFLNFINRPENAAQNAAYIGYATPNKKAKALLPDEIKNDPAFYPTDGIIKKLEVY
                     DNLGSRWLGIYNDLYLQFKMYRK"
     gene            complement(6029..6805)
                     /locus_tag="VGT87_001189"
     CDS             complement(6029..6805)
                     /locus_tag="VGT87_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ3814043.1"
                     /translation="MKKFANLYLTSVFVLLYIPIFYLIFYSFNKGGDMNGFTGFTLEH
                     YQTMFEDSRLMTILLQTFVLAFSSALLATIIGTFGAIFIHHVRGKYQNAMLSANNVLM
                     VSPDVMIGASFLILFTSLKFQLGMSSVLLSHIAFSIPIVVLMVLPRLKEMNQDMVNAA
                     YDLGANYFQMLKEVMLPYLTPGIIAGYFMAFTYSLDDFAVTFFLTGNGFTTLSVEIYS
                     RARQGISLDINALSTIVFFFSILLVIGYYYMSQDKEEKHA"
     gene            complement(6802..7596)
                     /locus_tag="VGT87_001190"
     CDS             complement(6802..7596)
                     /locus_tag="VGT87_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ3814044.1"
                     /translation="MKKTSSLFSIPYFLWILFFVVAPVTLLFYKSFFDIEGRVTLANY
                     ETFFSSWTYLRMSVNSILYAGIITLVTLLISYPTALFLTRLKHKQLWLMLIILPTWVN
                     LLLKAYAFMGIFGQQGGINSFLTFMGIGPQQILFTDFSFIFVASYIELPFMMLPIFNA
                     LDDIDHNVINASRDLGASEFQAFSKVIFPLSLNGVRAGVQSVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLTTQNWGMGSTIGVVLILTMIAIMWLTKEKSK"
     gene            complement(7580..8734)
                     /locus_tag="VGT87_001191"
     CDS             complement(7580..8734)
                     /locus_tag="VGT87_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ3814045.1"
                     /translation="MTKPIITFNNVSKTFEDSGTQVLKNINFDLEEGKFYTLLGASGS
                     GKSTILNIMAGLLDASSGDIYLDGERINDLPINKRDIHTVFQNYALFPHMTVFENVAF
                     ALKLKKVDKKEIAKRVKETLKMVQLEGYENRSIQKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTEMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNEGEIVQ
                     SGTPVDIYDEPINHFVANFIGESNIINGTMIEDYLVSFNGKEFESVDGGMRPNEPVEV
                     VIRPEDLQITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIEGEVIG
                     LDFTPEDLHIMRLNETEEEFDARIEEYVEMDEPEDGLINAIEEERNEENL"
     gene            complement(8780..9667)
                     /gene="murB"
                     /locus_tag="VGT87_001192"
     CDS             complement(8780..9667)
                     /gene="murB"
                     /locus_tag="VGT87_001192"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEQ3814046.1"
                     /translation="MITELHGIDIRENEPLKHYTYTKVGGPADFLAFPRNHYELSRIV
                     AYANKENMPWLVLGNASNLIVRDGGIRGFVIMFDKLNAVHLNGYTLEAEAGANLIETT
                     KIAKFHSLTGFEFACGIPGSIGGAVFMNAGAYGGEISHIFLSAKVLTSSGEIKTISAR
                     DMAFGYRHSAIQETGDIVISAKFALKPGNYDTISQEMNRLNHLRQLKQPLEFPSCGSV
                     FKRPPGHFAGQLIMEANLKGHRIGGVEVSEKHAGFMINVADGTAKDYEDLIAYVIETV
                     ENHSGVRLEPEVRIIGENL"
     gene            complement(9817..10317)
                     /gene="folK"
                     /locus_tag="VGT87_001193"
     CDS             complement(9817..10317)
                     /gene="folK"
                     /locus_tag="VGT87_001193"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEQ3814047.1"
                     /translation="MTIVYLSLGTNMGDRAAYLQKALEALADLPQTRLLAQSSIYETT
                     AWGKTGQADFLNMACQLDTQLTAADFLKETQAIEQSLGRVRHEKWGSRTIDIDILLFG
                     EEVYDTKELKVPHPYMTERAFVLIPLLELQPDLKLPPNHKFLRDYLAALDQSDITLFS
                     AQQTEF"
     gene            complement(10314..10673)
                     /gene="folB"
                     /locus_tag="VGT87_001194"
     CDS             complement(10314..10673)
                     /gene="folB"
                     /locus_tag="VGT87_001194"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEQ3814048.1"
                     /translation="MDKIVLEGCRFYGYHGAFKEEQTLGQIFLVDLELSVDLQAASLS
                     DQLTDTVHYGMVFDSVRQLVEGGKFILIERLAGAICEQLFNEFPPIEAIKVAIKKENP
                     PIAGHYKAVGIELERQR"
     gene            complement(10689..11480)
                     /gene="folP"
                     /locus_tag="VGT87_001195"
     CDS             complement(10689..11480)
                     /gene="folP"
                     /locus_tag="VGT87_001195"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEQ3814049.1"
                     /translation="MKIGRFVIEGNAAIMGILNVTPDSFSDGGSYTTVQKALDHVEQM
                     IADGAKIIDVGGESTRPGCRFVSATDEIDRVVPVIKAIKENYDILISIDTYKTETARA
                     ALEAGADILNDVWAGLYDGQMFALAAEYDAPIILMHNQDEEVYQEVTQDVCDFLGNRA
                     QAALDAGVPKNNIWIDPGFGFAKSVQQNMELLKGLDRVCQLGYPVLFGISRKRVVDAL
                     LGGNTKAKERDGATAALSAYALGKGCQIVRVHDVKANQDIVAVLS"
     gene            complement(11489..12073)
                     /gene="folE"
                     /locus_tag="VGT87_001196"
     CDS             complement(11489..12073)
                     /gene="folE"
                     /locus_tag="VGT87_001196"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEQ3814050.1"
                     /translation="MKRERLMSINKEKAEAAIYQFLEAIGENPNREGLLDTPKRVAKM
                     YAEMFLGLGKDPKEEFTAVFKEQHEDVVIVKDISFYSICEHHLVPFYGKAHIAYLPSD
                     GRVTGLSKLARAVEVASKRPQLQERLTSQIADALVEALNPKGTLVMVEAEHMCMTMRG
                     IKKPGSKTITTTARGLYKESRAERQEVISLMTKD"
     gene            complement(12108..13385)
                     /locus_tag="VGT87_001197"
     CDS             complement(12108..13385)
                     /locus_tag="VGT87_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEQ3814051.1"
                     /translation="MTYEETLEWIHDHLVFGIKPGLKRMLWVLGQLGNPQKNVKGVHI
                     VGTNGKGSTVNHLQHIFTTAGYEVGTFTSPYIMDFKERISINGRMISEKDLVIAANRI
                     RPLTERLVQETDFGEVTEFEVITLIMFLYFGDMHPVDIAIIEAGLGGLYDSTNVFQAM
                     VVVCPSIGLDHQAILGETYADIAAQKAGVLEGGETLVFAVENPSAREVFLTKAEQVGA
                     SIWEWQEQFQMAENASGYRFTSPLGVISDIHIAMPGHHQVSNAALAIMTCLTLKDRYP
                     RLTSDHIREGLANSLWLGRTELLAPNLMIDGAHNNESVAALVAVLKNNYNDKKLHILF
                     GAIDTKPIADMLVALEQIGDLQVTGFHYPNAYPLEKYPERFGRVADFKDFLALRKHAK
                     ADDFFVITGSLYFISEIRRYWKKHIEKNVLLTH"
BASE COUNT         4241 a         2983 c         2415 g         3985 t
ORIGIN      
        1 aaagtataac tttctctttt ttcatatatt ctatgattta aggactaccg acctgacgct
       61 tttagcgaca atctcagcga ctccttcttt aaaggcatct ggaataatat tagtagtgct
      121 caaagcgtca tctgggacaa gactggcaat accttttgcg gcagcaattt gcatctcaac
      181 agtaatggtt tttgcccgtg catctaatgc tcccctaaaa atacctggaa aggcaaggac
      241 attattaatc tggttaggaa aatcactgcg gccagtacct acaatgtaag cccccgcttc
      301 aagggcttca tcagggtaaa tctctggaat agggttggcc atggcaaaaa ttactgggcg
      361 cgctgccatt ttactaatcc actctgcttt taagactcct ggtgctgata cgccaatgaa
      421 gatatctgct ccttcaagag catcttctaa ggtacctgat ttaaactcac gattagtgac
      481 tttcgctata tccaaatgat gtggcgctaa ttgagcggct tcttgttcat tgataatgcc
      541 aaatttatcc actactgtca ctttcgtagc acctgctgct aataatttac gggtaatgga
      601 taatcccgca gatccaccac cgttcaccac gatggatacc tcatcaaggg atttttgaag
      661 aagtttcaaa ctattaaaga tggctgctaa caccacaata gctgttccat gctgatcgtc
      721 atggaaaaca ggaatatgac attccttaat taagcgttgc tcaatttcaa aacagcgggg
      781 cgcactaata tcctctaaat taataccacc aaaggttggt gcaagcgcct tgacaatact
      841 aataatttct tcagtatctt tggtatctaa tacaatcgga atagcatcga cacctgcaaa
      901 tgctttaaaa agggctgctt ttccttccat aactggcatg gctgcttcag ggccaatatc
      961 ccccagacca agaaccgcag taccatcact gataacggct actgtatttt tcttggtggt
     1021 taagtcatat gctaatgttt tatcttttgc gatggcagat gacacaccag ctacacctgg
     1081 tgtataggca atgctcaaat catgtttagt tttaatgtca acttttggtt gaacttccaa
     1141 tttaccgccg aacgttttag cttgttcaag ggcaagttga cctaattgat ttttcatagg
     1201 aaatacctct cttttttatt ggtttggatt aactaaaaat aattcttaaa atagctgtca
     1261 tggtgataac ggtaatggcc ccgcctagac gagtagcgac ttgggcaaac ggcattagat
     1321 tcatgcggtc agccgtactc aaaatagcca cgtcccctgt tccacccata ccactttgac
     1381 aagctgaaat aatagctgct tccactgggt tcatgttaag gaaacgagag acaaaaaaac
     1441 cgacactgac aaccgttaac accacactaa tcaccacaat aaaatattgc cagctaaggg
     1501 ttgccacaac ctcttttaag gggatataaa gcagaccaag ccctgccatt aacggaaaag
     1561 taaagttgct ggaaataaat ttataaagtt gtttggcacc attttgggtt tcttggggaa
     1621 taacattcag gtatttcaag aaggctgcca gaataatcat caacaccggt cctggaaaac
     1681 ctgttaaatg ctgtaacaag ccaccagcaa taaaaagtga acaagctgtt aaaacgcctg
     1741 cccccattaa tttaacatct agggctccag aattgtcttt tagggcatca gacatgtctt
     1801 cgctgtgacc aattttaacc agttgccctt gaccagaata acttgtgtgc ttctctccaa
     1861 aacgattgag caaagccgtg cacaagatag caaagaaatt cccaataatg gtggctggaa
     1921 tcagttgagc cactaattgc tcacttccga ctcctgtaat ggcactgtaa ccaagagata
     1981 agggtaaaat accttcacca attcctccag caaggactgg cgtcacaaca taaaagaggg
     2041 tatgttgcca gtctaatcct aaaatgacgc caaccaaagt ccctacaccc attgcacaaa
     2101 ccattccaag taacatggga ataatcatcc gaaaaagacc ttgaatcaag atttttcgat
     2161 tcatgcctaa aatactaccg cagaccaggc aggcaatata aaaataaaga aaattggctt
     2221 gcttcatcaa gacattcgtc gcttctaaga cattggggtt gaggagatta aaaaaaacca
     2281 aaatcgaagg gactaagagt gataaaatag ctgggccacc aaaatgtttg aggcctggaa
     2341 tggtcgctcc aatggttcct agtagccaac caagggttaa aataacagca aagccaccca
     2401 gcatattaac aggcagctgt tggagccagc ctgtgactaa aattaaggta gctaaaacca
     2461 aataaacagt caagggaacg gagcctattc gcctattttg ccatgctttt gaatgctcag
     2521 acaagtcttt ttgaggcatt ttcttgctta ttgtttccat gaaacaacct cctttagtga
     2581 taaggctagt ataaaagaga acatgtcaaa agcatagttt atttaagctg tttaactact
     2641 ttaatttttt caagttattt aattaattta aactagcagg tcatttctta tcgtgttata
     2701 ctcaataaag aggagacctt atgaaaaaac cactacgttt atgggccagt ttatcgttga
     2761 ttttggtatc tatgattgtt gtcacaacct ctctatttta cgggattatg ctacatgata
     2821 cgcaccaatc catcaaaaat caggaaaccc atttgctaac gtcaactgga aaaatgttag
     2881 caagtcatca agcaattaaa gaattactcc taaacaacca accaaatgct aaaacaacag
     2941 cctataccaa ctccattgcc tccatctata accttgatta tgtggtcgtg atgaacatga
     3001 aagggattcg actcacccat cctaacccga aaaatatcga aaaacctttt caaggaggtg
     3061 atgaggaggc tgttctagca ggaaaaaagg ttatctctac cgccaagggc actttaggaa
     3121 agtcacttcg ctatctagta cctgtgtttg acggtgacaa acagattggt gctattgcgg
     3181 ttggcattaa actaactacc ctaaatgatg tggctttaac gtcaaaacgc aattataccc
     3241 tgtctttact cctttgtctt cttattagtt tactggtcac cagttttatt tcttttcgac
     3301 tcaagcgtca actgcaccag ttagaaccaa gtgaaattta ccagctcttt gaagagcgta
     3361 atgccatgtt ggatcaaatc gaagccgccg tatttgtggt agataaagct ggtatcttac
     3421 agctctgcaa tcaggctgga caaaaattaa ttgctcggaa atgccagttg ggaaaaccta
     3481 ctggcaacag tttcaatcac ctctttcctg attttccaaa acttagtttg caagaggggc
     3541 atgaacaact ctttcggtat ggtgaggaag actacttgtt agccatttca cccatctgtg
     3601 tcaaaaacga tcaccgaggc cacattattt tcatgcgaga agcagttaag gctattgata
     3661 ccttggatca gctagcctat accaccgcct atgcttctgc tctacaagcc caaactcata
     3721 aattcatgaa tcaattgcat gttatttatg gcttagtgga tattgcttat tatgaccaat
     3781 tgaaaattta tttagacagt attttggagc ccgaaaatga aattctcact agcttatctg
     3841 tccttgttaa ggagcctctt ctagctagtt ttctcattgg agagcaagag aaataccaag
     3901 aattaaatgt ccatctcaag attgatgttt taagcgagat tcctcattct gccaccaaga
     3961 atcaacttaa taatggtttg atgatttacc gctttatcca tactaatctt ctaaccacac
     4021 tgagacccaa atcactcgtt ttgagtattc aacatgatca gaatcattta atcagccatt
     4081 acaccttgac agacaattgg attgatttgg agcgggtaca gcctattttt gacttacctt
     4141 attttcaaca gctcttgacc gatacaaatt ccaaattcca tcaacatcaa accgtaaccg
     4201 agttacagtt ctcagtgaca acaccttatg taggagggca acatgaacgt tttaatcatt
     4261 gaagatgatc ccatggtgga ttttatccat cgcaattacc tcgaaaaact aaatcttttt
     4321 gatcgcatta tatccagcga ctctatgaaa gctgttcaaa gcatacttac tgattacgca
     4381 attgacctca ttttactgga tattcatatt acagatggca atgggattca atttttggag
     4441 aaatggcgtg ctcagcatat tccttgcgaa gtcattatta tttcagccgc aaatgatggg
     4501 aatattatcc gagatggttt tcatttagga attatcgact acctcatcaa gccctttacc
     4561 tttgaacgct ttcaagaaag catccaacag tttgttacac atcgagaaca tttggccaat
     4621 cagcaactag agcaggccca aattgaccaa ctgaaatgtt tgactagcaa aaaagacact
     4681 aaaaacaaac aattgttaga aaaaggctta tctgaatcca ctttccagtg gattatggaa
     4741 aatatcaaag cctttgatca accttttact atccaagaat tagcttccgc ttgtcactta
     4801 tcccatgtct ctgtccgtaa atacattgct tatttagaag aaaataagca actgaacagc
     4861 cagcaaattt ttaccaaggt tggccgtccc tatcgcgtgt actggtgacg tcatctgtcg
     4921 atacattaaa aaggctggag aaataccagc ctttttgagt gcttatttgc gatacatttt
     4981 aaattggagg tataaatcat tataaatccc caaccatctt gaccctaaat tatcataaac
     5041 ttccaatttt ttgataatgc catctgttgg ataaaaagca ggatcatttt ttatctcatc
     5101 tggaagtaag gccttggctt ttttatttgg tgtcgcataa ccaatatatg cagcattttg
     5161 cgcagcattt tcaggacgat tgataaagtt caaaaaagca tacgcttctt tttcatgttt
     5221 catggttttt ggtagtacca aattatcaaa ccaaaggtta gacccttctg aaggcacgat
     5281 gtagtgaagg tgttcgttac tatctaacat ctcactggct tcaccagaaa aggtaattcc
     5341 aatagcagcg tcaccttgaa tcatgtagcc tttcatctca tctgctacaa tggctttaac
     5401 attcggcgtc aactgctgca gttttctctc ggctgcctgc aactgctcta gatttttaga
     5461 attcacacta taaccaaaag ttgttaaacc aacccctagc atttcacgcg ctccatcaat
     5521 cagcataata ctatttttat attctggacg ccacagatct tcccagtgca taggcgcctt
     5581 atcaactaat tgatcattat aaacaatccc aacggttccc cagaaataag gcaaagaata
     5641 gtcgttttgt gggtcaaagc ttttccctaa aaattctttc ccgatattat ccatgccaac
     5701 taattttgac ttatcaagct tattgagtag gttttctttg atcattttat caatggtgta
     5761 atcactagga acagcaatgt cgtaagtggt tccgccttgc ttgattttag tgtacatggc
     5821 ttcattggaa tcgaaagttt catactgcac ttcaatgccc gtttctttgg tgaatttttt
     5881 gagcaaagct ggatcaatgt aatctcccca gttataaata actaatttat ccgattgact
     5941 accagaacct gattttttct gcaagatgaa agaaagactt gttaaaataa caataacacc
     6001 caaaactcct gctagaaagg aataaagttt acgcatgttt ttcctccttg tcctgtgaca
     6061 tataataata accgatcact aagaggatgg agaaaaagaa aacgatggtt gacaaagcat
     6121 tgatatccaa ggaaattccc tgacgagccc gcgaataaat ctcaacagat aaagtagtaa
     6181 aaccatttcc agtcaaaaag aaagtcactg caaaatcatc taaggaatag gtaaaggcca
     6241 taaaataacc tgcaataatc cctggtgtta agtatggcag catgacttct ttgagcattt
     6301 ggaaataatt agctcccaaa tcataagcgg cgttgaccat gtcttgattc atctctttca
     6361 agcgcggcaa taccatcaaa accacaatag gaatcgaaaa agcaatatga cttaataaaa
     6421 ctgaagacat gcccagctga aacttcaatg atgtaaaaag aattaaaaag gaagccccaa
     6481 tcatgacatc tggtgatacc atcaagacat tattggctga tagcatggca ttttggtact
     6541 tacctctaac atggtggata aagatagctc caaaggtccc aataatcgtt gctagtagag
     6601 cgctactaaa agcaagaaca aaggtttgca gtaagattgt catgagacga ctatcctcaa
     6661 acatggtttg gtaatgctca agggtaaatc ctgtaaaacc attcatatcc ccacctttgt
     6721 tgaaagaata gaaaatcaaa taaaaaatag gaatgtagag taaaacaaag acactcgtta
     6781 aataaagatt ggcaaatttt ttcatttact tttctccttt gtgagccaca taatagcaat
     6841 catggttaaa attaacacca cacctatggt tgatcccatt ccccagtttt gggtggtcaa
     6901 aaaatgttgt tcaatggctg taccaagtgt aatcacccgg tttccaccaa tcaaacgggt
     6961 taacataaag agactcaaac ttgggataaa gacagactga acacctgccc taaccccatt
     7021 taaagaaagg ggaaaaataa cttttgagaa ggcctgaaat tcactagctc ctagatcgcg
     7081 actggcattg atgacattat ggtcaatatc atccaaagca ttaaaaatcg gtaacatcat
     7141 aaaaggaagc tcaatgtaag aggctacaaa aatgaaggag aaatccgtga aaaggatttg
     7201 ctgcgggcca atccccataa aggttaaaaa gctgttaatc cctccttgtt gaccaaagat
     7261 tcccataaag gcataggctt ttagcaataa gtttacccaa gttggcaaaa taatgagcat
     7321 aagccacaac tgcttgtgct ttaggcgcgt taaaaagaga gccgtaggat atgaaattaa
     7381 gagcgtgacg agtgtgataa taccagcgta taaaatagaa ttcacactca ttctcaaata
     7441 ggtccaagag ctaaaaaagg tttcataatt ggctaaggtc acgcgccctt ctatgtcaaa
     7501 aaaggacttg taaaacaaga gagtgactgg tgccacaaca aagaagagaa tccataagaa
     7561 gtaaggaatc gaaaaaagac tagaggtttt cttcattacg ctcctcctca atggcattaa
     7621 tcaatccatc ttcaggctca tccatttcca catattcttc aatacgggca tcaaattcct
     7681 cttcagtctc attaagacgc atgatatgaa gatcttcagg ggtaaaatct aatccgataa
     7741 cttctccttc gatagctttg cgggtagaat gaatcatcca ttcattaccc aattcatcat
     7801 aggcaataat ttcgtagtga accccgcgga ataattgggt atcaacctta acttgtaatt
     7861 tcccttcttc tggcaaagta atttgaagat cttcaggacg aataaccact tcaacaggct
     7921 cattagggcg cattccaccg tccacagatt caaattcttt cccgttaaag gagacaagat
     7981 agtcttcaat catggtaccg ttaataatat tagattctcc aataaaatta gcaacaaaat
     8041 gattaattgg ctcatcgtaa atatccactg gtgttcccga ctgaacaatt tcaccttcat
     8101 tcatgacaaa aatccaatca ctcatggcca aggcttcttc ttgatcgtga gtaacaaaaa
     8161 caaaggtaat acctagacgt tgttgcaatt cacgtaattc atattgcatc tctgttctta
     8221 atttcaaatc aagggctgag agtggttcgt caagtaagac cacacgcggt tgattgataa
     8281 tcgcacgcgc aatggcaaca cgttgacgtt gaccaccaga taacttctgg atagaacgat
     8341 tttcataccc ttctaattga accattttca aggtttcttt cacgcgctta gcaatctctt
     8401 tcttgtccac tttcttcagc ttcaaagcaa aggcaacatt ctcaaaaaca gtcatatgag
     8461 ggaagagggc ataattttgg aaaacggtat gaatgtcacg tttattaatc ggcaaatcat
     8521 taatacgttc cccatctaaa taaatgtctc cgctgctggc atctagtagg cccgccataa
     8581 tatttaaaat agttgatttt ccagagccag aagctccaag cagggtgtaa aattttcctt
     8641 cttcaaggtc aaagttaatg ttctttagaa cctgtgttcc actgtcctca aatgtttttg
     8701 aaacattgtt aaatgtgata attggcttag tcaattgtca taaatcctcc acggtacttt
     8761 ctttaattat caataatggt tacaggtttt ccccaataat ccgaacttct ggttcaagcc
     8821 tgacaccaga atggttttca actgtctcaa tgacataagc aatcaaatct tcataatctt
     8881 tagctgtgcc atctgccaca ttaatcataa aaccagcgtg tttttcggaa acttccacac
     8941 caccaatccg atgcccttta agatttgctt ccatgattaa ttgacctgca aagtgtcctg
     9001 gcgggcgctt aaacacagat ccacaagatg gaaattctaa aggttgtttt agttggcgaa
     9061 ggtgatttaa ccgattcatt tcttggctga tcgtatcata attacccggt ttaagagcaa
     9121 acttagcaga aatgacaatg tcacctgttt cttgaatggc agagtgacgg taaccaaaag
     9181 ccatatccct agctgaaatg gttttgatct ctccacttga ggtaagcacc ttagcggata
     9241 agaaaatatg tgatatttcc ccaccatagg cacctgcatt cataaaaaca gcacccccaa
     9301 tgcttccagg aatgccacat gcaaattcaa accctgttaa actatggaat ttggcaatct
     9361 tcgttgtttc aatcagattg gcaccagctt cggcttctaa ggtataacca tttaagtgca
     9421 ctgcatttag cttatcaaac atgatgacaa agccacgaat accaccatct cgcacaatga
     9481 gattgctggc atttcccaaa acaagccaag gcatattttc tttgttggca taagcaacga
     9541 tgcgtgatag ctcgtagtga tttctaggaa aagccaagaa atctgctggg ccacctacct
     9601 tagtgtaagt gtaatgtttt aggggttcat tctctcgaat atcaatccca tgaagttcag
     9661 ttatcatagt tatcttcttt cttttatcac ttcttatacg cgtaaaaaaa tcggcataaa
     9721 gccgatacta ttatatcaaa ttttagaaaa aataacgatg aatttggcaa aactttatgg
     9781 attggcttat agcaggcatt actccgaaaa cagagtctag aactctgtct gttgagctga
     9841 aaagagcgtg atatctgatt gatctaaagc tgcaagataa tcccttaaaa acttatgatt
     9901 aggaggcaat tttaaatctg gctgcaattc caataagggg attaaaacaa aagcacgctc
     9961 agtcatataa gggtgtggca ctttcaattc ctttgtgtca taaacctctt ctccaaatag
    10021 caagatatca atgtcaatag ttcttgatcc ccatttttca tgcctcacac gaccaagaga
    10081 ttgctcaata gcttgtgttt cttttaagaa atcagccgcg gttaattgtg tatctaattg
    10141 acaggccata ttgagaaaat cagcctggcc tgtcttgccc caagcagtcg tttcataaat
    10201 ggatgattga gcaagcaacc gtgtttgagg taagtcagct aaagcctcaa gagctttttg
    10261 caagtaagct gctcggtccc ccatattggt gcctaaactt aaataaacaa tggtcatctc
    10321 tgtctctcca attcaatacc aactgcctta tagtggcctg caatcggtgg gttttccttt
    10381 ttaatggcca ctttgatggc ttcaataggt ggaaattcgt taaaaagctg ttcacaaatc
    10441 gcacctgcta aacgttcaat caaaataaac tttccccctt ccactaactg acggacacta
    10501 tcaaacacca tcccgtaatg gaccgtatct gttaattggt ctgacaaaga agctgcttgc
    10561 aaatcgactg acaattctaa atcaaccaga aaaatttgcc caagggtctg ttcttcttta
    10621 aaggctccat ggtagccata aaaacggcaa ccttctaata cgattttatc cataagtcct
    10681 cacatcaact agctcaaaac agccacaatg tcttgattag ccttgacatc gtgtacgcgt
    10741 acaatctgac agccttttcc aagggcataa gcagacaagg ctgctgtcgc tccgtctcgc
    10801 tctttagctt tggtgttgcc gcctaacaag gcatctacga cacgctttct cgaaatacca
    10861 aacaagacag gatagcccaa ctgacagacg cggtccaatc cttttaataa ctccatattc
    10921 tgttgaacag atttggcaaa tccaaatcct ggatcaatcc aaatattgtt ttttggcacg
    10981 ccagcatcaa gagctgcttg tgctctattg cctagaaaat cacaaacgtc ttgtgttacc
    11041 tcttgataaa cttcttcgtc ttggttatgc atcaagatga taggcgcatc gtactcggct
    11101 gctaaggcaa acatttgtcc gtcgtacaaa cctgcccaaa catcattgag aatatcggca
    11161 cccgcctcta aagctgctct agctgtttcg gttttatagg tatcaatgct gattagaata
    11221 tcatagtttt ctttgatggc cttgatcaca ggaaccaccc ggtcaatttc atcggtagcg
    11281 cttacaaaac ggcaacctgg acgtgttgat tctccaccaa cgtcgatgat tttagcacca
    11341 tcagcaatca tttgctcaac gtgatctaat gctttttgca cagtagtgta agacccccca
    11401 tctgaaaaag aatctggagt cacatttaaa atccccatga tagccgcatt gccctcaatc
    11461 acaaaccttc caattttcat aagtcctcct aatcttttgt cattagagaa ataacttctt
    11521 gtctctcagc acgactttcc ttgtataagc ctctggcagt ggtggtaatg gttttactac
    11581 ctggcttttt gatgcctcgc atggtcatgc acatgtgctc tgcttctacc ataactaaag
    11641 tccctttagg attgagggct tcaactaaag catccgctat ttgagaggtc aaacgctctt
    11701 ggagttgagg tcgtttacta gctacttcaa ctgctcgcgc taatttactc aaacctgtta
    11761 ctcgaccatc acttggtaag taagcgatat gagccttgcc ataaaaagga actaagtggt
    11821 gttcacatat agaataaaaa ctaatgtctt tgacaatcac cacatcctca tgttgttctt
    11881 taaagacagc cgtaaactcc tctttaggat cttttcccag tcccaaaaac atctccgcat
    11941 acattttagc aacccgttta ggcgtgtcaa gaagaccttc ccgatttgga ttttcaccaa
    12001 tagcctctaa aaactgataa atggctgctt ctgctttttc cttattaata gacattagtc
    12061 tttccctttt cattagtttt ttccttttcc ttttcatttt ctctttttta atgggttaaa
    12121 agaacgtttt tttcaatatg ttttttccaa taccgtctaa tttctgaaat aaaatatagt
    12181 gaccctgtaa tcacgaaaaa gtcatctgct ttagcatgct tacgcagggc caagaaatct
    12241 ttgaaatcag caaccctacc aaaacgttct gggtattttt ccaatggata ggcgttaggg
    12301 taatgaaagc cagtaacctg caagtcacct atttgctcaa gagcaaccaa catatccgca
    12361 ataggcttgg tgtcgatcgc accaaacaaa atatgaagct ttttatcatt gtagttattt
    12421 tttaagacgg ctaccaaagc agcaacgctt tcattgttat gagccccatc aatcattaga
    12481 tttggtgcta ataattcggt acggcctagc cacaaactat ttgccagacc ttcccttata
    12541 tgatctgaag ttagcctagg atagcggtct ttcaaggtta agcaagtcat aatagctaaa
    12601 gctgcattag acacttggtg gtgtcctggc atagcgatgt gaatatctga aataactcct
    12661 aaaggactag taaaacgata gccagatgcg ttctcagcca tttgaaactg ctcttgccat
    12721 tcccagattg aagcaccaac ctgctcagct tttgttaaaa aaacctctcg agctgacggg
    12781 ttttcaacgg caaaaacaag ggtctcgccc ccttctaaaa caccagcttt ttgggcggca
    12841 atatcagcgt aagtctctcc caaaatagct tggtgatcta aaccaattga gggacatacc
    12901 acaaccattg cttgaaaaac attggtcgaa tcataaagac cgccaaggcc tgcttcaata
    12961 atagcaatat ctacaggatg catgtctcca aaatagagga acatgatcaa agtaatcact
    13021 tcaaattcag ttacctctcc aaaatcagtc tcttgaacta gacgctctgt caaaggacga
    13081 atacgattag cagcaataac caaatctttt tctgaaatca ttcgaccatt tatgctaata
    13141 cgttctttga aatccataat ataaggagag gtaaaggtcc ccacttcata accagctgtc
    13201 gtaaaaatgt gttgtaaatg attgaccgtg gaaccttttc cgttagttcc aacgatatgc
    13261 actcccttga catttttttg gggatttcct aactgtccaa gaacccaaag catacgtttt
    13321 aagcctggct tgatgccgaa aactaagtga tcatgaatcc attctagagt ctcttcataa
    13381 gtcatttcac acctcacatc tatctttagt ataccatata taaaaaaaag tgacagcaga
    13441 taacctagag tagtaccctt tttttattat cctcaccaaa cactcaaata agttctaaag
    13501 tacactacac ttttatcttc attgcttttt ttacgtgata agaataccag ccccacatag
    13561 tctttgctac ctcctaaagt gttatctgtg acaataattt ttttactcct taatacacgt
    13621 tctt
//
LOCUS       DAUCLR010000050        13121 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.050, whole genome shotgun sequence.
ACCESSION   DAUCLR010000050 DAUCLR010000000
VERSION     DAUCLR010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13121
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.050"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(14..661)
                     /locus_tag="VGT87_001198"
     CDS             complement(14..661)
                     /locus_tag="VGT87_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEQ3814052.1"
                     /translation="MTPNKEDYLKCIYEIGEQEPKITNKMVAEKMHVSAPAVSEMIKK
                     MISQGWIVKDKAKGYLLKDKGYALVANLYRKHRLIEVFLIHQLGYNTQEVHQEAEVLE
                     HTVSDTFIDRLDKILDFPDFCPHGGTIPRYGQPLVEMNTTTLNTITELGRFRLSRIHD
                     HFDLIQYLETHHLSINTELTLTQIDTFAKTYTICYGDKELVIPENIAKQLYVTAL"
     gene            807..1739
                     /locus_tag="VGT87_001199"
     CDS             807..1739
                     /locus_tag="VGT87_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_101244918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEQ3814053.1"
                     /translation="MGKRMSLILGAFLSVFLLVACSSTGTKTAKSDKLKVVATNSIIA
                     DMTKAIAGDKIDLHSIVPIGQDPHEYEPLPEDVEKTSNADVIFYNGINLEDGGQAWFT
                     KLVKNAQKTKNKDYFAVSDGIDVIYLEGASEKGKEDPHAWLNLENGIIYSKNIAKQLI
                     AKDPKNKETYEKNLKAYVAKLEKLDKEAKSKFDAIAENKKLIVTSEGCFKYFSKAYGV
                     PSAYIWEINTEEEGTPDQISSLIEKLKVIKPSALFVESSVDRRPMETVSKDSGIPIYS
                     EIFTDSIAKKGKPGDSYYAMMKWNLDKISEGLAK"
     gene            1803..2528
                     /locus_tag="VGT87_001200"
     CDS             1803..2528
                     /locus_tag="VGT87_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004218966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEQ3814054.1"
                     /translation="MITTNNLCVTYDGNSNALEAINVTIEGPSIVGIIGPNGAGKSTF
                     MKAILNLIDYQGHVTVDGKDGRKLGHTVAYVEQRSMIDYNFPITVKECVALGTYSKLG
                     LFRRVGKKQFEQVDKVLKQVGLEDFGHRPIKSLSGGQFQRMLVARCLIQESDYIFLDE
                     PFVGIDSVSEKIIVDLLKELKMAGKTILIVHHDLSKVEHYFDKLMILNKHLVAYGNVC
                     EVFTVDTLSKAYGNHLILGKEMV"
     gene            2529..3383
                     /locus_tag="VGT87_001201"
     CDS             2529..3383
                     /locus_tag="VGT87_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEQ3814055.1"
                     /translation="MSMKFFEGLMSYHFLQNALITAVVIGIVSGAVGCFIILRSMSLM
                     GDAISHAVLPGVALSFILGVNFFIGAIIFGLLASVIITYIKENSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDSDKWITIGVSIFVLGVISLFFKELLLT
                     SFDPILAKSMGVKVNAYHYLLMVLLTLVAVTAMQSVGTILIVALLITPAATAYLYANS
                     LKVMLVMSSLLGALASVLGLYLGYTFNVAAGSSIVLTSAMMFLISFFVSPKQGYLKRW
                     MQKKEKTP"
     gene            complement(3531..4337)
                     /locus_tag="VGT87_001202"
     CDS             complement(3531..4337)
                     /locus_tag="VGT87_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEQ3814056.1"
                     /translation="MKKLLSLSLVAISLLSLSACESVDRAIKGDKYIDEKTAKEESEA
                     ASKAYEESIQKALKADASQFPQLTKEVGKEEAKVVMRTSQGDITLKLFPKYAPLAVEN
                     FLTHAKKGYYDNLTFHRVINDFMIQSGDPKGDGTGGESIWKGKDPKKDAGNGFVNEIS
                     PFLYHIRGALAMANAGANTNGSQFYINQNKKNQSKGLSSTNYPKPIISAYEHGGNPSL
                     DGGYTVFGQVIDGMDVVDKIAATSINQNDKPKQDITITSIDIVKDYRFKN"
     gene            4554..6959
                     /locus_tag="VGT87_001203"
     CDS             4554..6959
                     /locus_tag="VGT87_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEQ3814057.1"
                     /translation="MVKRNQRKKSAPKKRLTKAEVEKQRAIKRMILSVLMALLLIFAM
                     LRLGVFGVTTYNMIRFLVGSLAYPFMFAWLIYLFCFKWLRQKDGMIAGVVIAFLGLLV
                     EWHAFLFAMPRMLDQAIFLGTARLITRDLLALRVTEFVGGGMLGALLYKPIAFLFSNI
                     GSYFIGFLFILLGLFLMTPWDIYDVSHFVKEAVDKLAVAYQENKEKRFIKREEHRLQA
                     EKEALEKQAQEEEKRLAELTVDPETGEIVEDSQSQVSYDLAEDMTKEPEILAYDSHLK
                     DDEASLFDQEDLAYAHEEIGAYDSLSALASSEDEMDMDEPVEVDFTPKTHLLYKLPTI
                     DLFAPDKPKNQSKEKNLVRKNIKVLEDTFQSFGIDVKVERAEIGPSVTKYEIKPAVGV
                     RVNRISNLADDLALALAAKDVRIEAPIPGKSLIGIEVPNSEIATVSFRELWEQSDANP
                     ENLLEVPLGKAVNGNARSFNLARMPHLLVAGSTGSGKSVAVNGIISSILMKARPDQVK
                     FMMIDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFSKIGVRNIA
                     GYNTKVEEFNASSEQKQIPLPLIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGIH
                     MILATQRPSVDVISGLIKANVPSRMAFAVSSGTDSRTILDENGAEKLLGRGDMLFKPI
                     DENHPVRLQGSFISDDDVERIVNFIKDQAEADYDDAFDPGEVSDNDPGFSGNGGAAEG
                     DPLFEEAKALVLETQKASASMIQRRLSVGFNRATRLMDELEEAGVIGPAEGTKPRKVL
                     QTN"
     gene            complement(7029..7382)
                     /locus_tag="VGT87_001204"
     CDS             complement(7029..7382)
                     /locus_tag="VGT87_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEQ3814058.1"
                     /translation="MMIYKLTALAFLIVTPLFAMIVTSLFKLNKKGFKFPDVALLLFA
                     IEIVLVSGKFFTHNLLPYYLIIMSLLAIVISLLLVIRTQSFSYHRFMKLFWRVGFLVT
                     VIFYLILVIFIFTLA"
     gene            7626..8051
                     /gene="rplK"
                     /locus_tag="VGT87_001205"
     CDS             7626..8051
                     /gene="rplK"
                     /locus_tag="VGT87_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEQ3814059.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFDFITKTPPAAVLLKKAAGVEKGSGTPNTTKVATVTR
                     AQVQEIAETKMPDLNAANIEAAMRMIEGTARSMGFTVTD"
     gene            8157..8846
                     /gene="rplA"
                     /locus_tag="VGT87_001206"
     CDS             8157..8846
                     /gene="rplA"
                     /locus_tag="VGT87_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEQ3814060.1"
                     /translation="MAKKSKQMRAALEKVDSTKAYSVEEAVALVKETNFAKFDASVEV
                     AYNLNIDVRKADQQIRGAMVLPNGTGKTQRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINGGWLDFDVVIATPDMMAIVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADKAGNVQALIGKVSFDADKLVENFKAFHDVMAKAKPATAKGTYMANVSITSTQ
                     GVGIKVDPNSL"
     gene            9169..9897
                     /locus_tag="VGT87_001207"
     CDS             9169..9897
                     /locus_tag="VGT87_001207"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEQ3814061.1"
                     /translation="MEPKYQRILIKLSGEALAGEKGVGIDIPTVQAIAKEIAEVHVSG
                     VQIALVIGGGNLWRGEPAADAGMDRVQADYTGMLGTVMNALVMADSLQHYGVDTRVQT
                     AIPMQNVAEPYIRGRALRHLEKNRIVVFGAGIGSPYFSTDTTAALRAAEIEADAILMA
                     KNGVDGVYNADPKKDANAVKFDELTHGEVIKRGLKIMDATASTLSMDNDIDLVVFNMN
                     EAGNIQRVVFGEHIGTTVSNKVCD"
     gene            9927..10484
                     /gene="frr"
                     /locus_tag="VGT87_001208"
     CDS             9927..10484
                     /gene="frr"
                     /locus_tag="VGT87_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEQ3814062.1"
                     /translation="MANAIIETAKERFAQSHQSLSREYASIRAGRANASLLDRIQVDY
                     YGAPTPLNQLASITVPEARVLLISPFDKSSIKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRKELAKEVKKVGENAKIAIRNIRRDAMDDAKKQEKAKEITEDELKTLEKDI
                     QKATDDAIKEIDRMTAEKEKELLSV"
     gene            10592..11449
                     /locus_tag="VGT87_001209"
     CDS             10592..11449
                     /locus_tag="VGT87_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEQ3814063.1"
                     /translation="MNDLLATVITGLIKEENANDYFIQKEGFTFTLSKAEGERQIGDM
                     VTGFAYTDIKQKARLTTKEIRSTRTSYGWGEVTEVRRDLGVFVDTGIPNKEIVVSLDV
                     LPEMKELWPKKGDKLYIRLEVDKKDRIWGLPAEPEVFQKMASPAYNNMQNQHWPAIVY
                     RLKLTGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMIVTYLEANGGFMTLNDKSSPEEIKATFGISKGQFKKALGGLMKAKRIKQ
                     DATGTELIG"
     gene            11522..12031
                     /gene="msrA"
                     /locus_tag="VGT87_001210"
     CDS             11522..12031
                     /gene="msrA"
                     /locus_tag="VGT87_001210"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEQ3814064.1"
                     /translation="MERAIFAGGCFWCMVQPFEEQAGILSVRSGYTGGHLPNPSYEQV
                     CAKTTGHTEAVEIIFDPKQIAYKDLVELYWAQTDPTDAFGQFEDRGDNYRPVIYYTTE
                     RQKEIAEQSKANLQASGRFDQPIVTTIEPAEPFYLAEDYHQGFYKKNPKRYAQSSAIR
                     HQFLEENWS"
     gene            12028..12243
                     /locus_tag="VGT87_001211"
     CDS             12028..12243
                     /locus_tag="VGT87_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEQ3814065.1"
                     /translation="MRKSFYSWLMTQRNPKSNEPVAILADLVFDDTTFPKHTNDFELI
                     SRYLEDQASFSFNLGQFDEIWEDYLAH"
     gene            12399..>13121
                     /locus_tag="VGT87_001212"
     CDS             12399..>13121
                     /locus_tag="VGT87_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEQ3814066.1"
                     /translation="MIITKKSLFVTSVALSLAPLATAQAQEWTPRSVTEIKSELVLVD
                     NVFTYTVKYGDTLSTIAEAMGIDVHVLGDINHIANIDLIFPDTILTANYNQHGQATTL
                     TVQAPASSPASVSHVPSSEPLPQASATSQSTVPMAPSATPSDVPTTPFASAKPDSSVT
                     ASYELTSSTNDVSTELSSESQKQPEVPQEAVPTPKAAETTEVEPKTDISEDSTSANRP
                     VPNESASEEVSSAAPAQAPAEKE"
BASE COUNT         4126 a         2288 c         2806 g         3901 t
ORIGIN      
        1 gagacgcaag gggttaaagg gctgtgacat agagttgctt agcaatattt tcagggatga
       61 caagttcctt gtcgccataa caaatcgtat aggtttttgc aaaggtatca atttgagtta
      121 aggttagctc ggtattgata ctcaggtgat gtgtttctag atactgaatt aaatcaaagt
      181 ggtcgtggat tctgcttagg cgaaaccgtc ccaactccgt gatggtgttg agagttgtag
      241 tgttcatttc gacaagaggc tgaccatagc gaggaatagt ccctccgtgt gggcagaaat
      301 ctggaaaatc aaggatcttg tctaaacggt cgataaaggt gtctgagaca gtgtgctcta
      361 agacttcagc ttcttggtgc acttcttggg tattatagcc cagctgatga atcaaaaata
      421 cctcaatcaa gcggtgtttg cggtagaggt tagcaacaag agcatagcct ttgtctttta
      481 gcaaatagcc ttttgcttta tctttgacaa tccagccttg tgaaatcatt tttttaatca
      541 tctctgaaac ggcaggtgcc gaaacgtgca tcttttctgc aaccatttta tttgtaatct
      601 tgggttcttg ttctccaatt tcatagatac atttcaagta atcttcttta ttaggcgtca
      661 tatcgtttgg cctttctctt atttataatg attattatat cagaattttt aggaaaaagc
      721 ttgacaaaaa gcctaaaaga ggatatcctt atagcataat taactaaact taattaaata
      781 aataatatat cctaaggaga gaaatgatgg gaaagagaat gagcctcatt ttgggtgctt
      841 ttttatctgt ttttctttta gtagcttgtt cttcaactgg tactaaaact gctaagagtg
      901 ataaattaaa agtcgtggca accaattcaa ttattgccga catgacaaaa gctattgctg
      961 gtgataaaat cgatctgcac agcattgtgc caatcggtca agatcctcat gagtacgaac
     1021 cattaccaga agatgttgaa aaaacaagta atgctgatgt gattttctat aatggtatca
     1081 atctagaaga tggtgggcaa gcttggttca ccaaactagt gaaaaatgct caaaaaacga
     1141 aaaacaaaga ttactttgcc gtgtctgatg gcattgatgt gatttacttg gaaggtgcaa
     1201 gcgaaaaagg aaaagaagat ccacatgctt ggttaaatct cgaaaacgga atcatttatt
     1261 caaaaaacat tgccaaacaa ttgattgcaa aggatcctaa aaacaaagaa acttatgaaa
     1321 agaacctaaa agcttatgtg gctaaattgg aaaaactaga caaagaagcc aaatcaaaat
     1381 ttgatgctat tgcagaaaat aaaaaattga ttgtgactag tgaaggttgc ttcaagtact
     1441 tttcaaaagc ttacggtgtc ccatctgctt atatctggga aattaacacc gaagaagaag
     1501 gaacaccaga tcaaatttca tcattgattg aaaaactaaa agtcatcaag ccatctgcgc
     1561 tttttgtaga gtcaagtgtc gatagacgcc ctatggaaac tgtttctaaa gatagtggta
     1621 ttcctattta ttctgagatc tttacagatt caattgctaa aaaaggtaaa cctggcgata
     1681 gttattatgc tatgatgaaa tggaaccttg acaaaatttc tgaaggtcta gcaaaataag
     1741 ataacaataa agcacagcga ttttagtgtt gtgctttgtt ttatttatgt aaaggagaca
     1801 aaatgattac aactaacaat ctttgtgtga cctacgatgg caacagtaat gctttagaag
     1861 cgattaatgt gacaattgaa ggtccttcta tcgttggtat tatcggtcct aatggagctg
     1921 ggaaatcaac ctttatgaaa gctattttaa atcttattga ttatcaaggt cacgtaacgg
     1981 ttgatggcaa agatggtcgt aagcttgggc atacagtagc ttacgtagaa caacgaagca
     2041 tgattgacta taattttcct attactgtta aagaatgtgt ggcgctaggg acttatagta
     2101 aattaggcct ttttcgccga gtaggaaaaa aacaatttga gcaggttgat aaggttctca
     2161 aacaagttgg tttagaagac tttggccatc gaccgataaa atccttatct ggtggtcagt
     2221 ttcagcggat gttggttgcg cgttgcttga ttcaagaatc agactatatt ttcttagatg
     2281 aaccgtttgt tggcattgat tcggtcagcg aaaaaatcat tgtagatctt ttgaaagagc
     2341 tcaaaatggc tggcaaaacg attttaattg tacatcatga tttgagcaaa gtagagcatt
     2401 attttgacaa actcatgatt ttaaataaac atttggtggc ttatggcaac gtttgtgagg
     2461 tatttactgt tgataccttg tcaaaagctt atggcaatca tttgatttta ggaaaggaga
     2521 tggtgtaaat gtctatgaaa ttttttgagg gcttgatgtc ataccacttt ttacaaaatg
     2581 cactgataac ggctgtagtc attggtatcg tctcaggtgc tgtagggtgt tttattatcc
     2641 ttaggtcaat gtctcttatg ggtgatgcca tctcacacgc tgttttacca ggggttgctt
     2701 tgtcatttat tttaggagtc aattttttta ttggagcgat tatttttggg ttattagctt
     2761 ctgttattat tacttatatc aaggaaaact ctgtcattaa aggagatacg gctattggta
     2821 tcacctttag ctcttttttg gcactgggag tcatcttgat aggggtagcc aatagttcga
     2881 cggacttatt tcatattttg tttgggaata ttttagctgt gcaagacagc gataagtgga
     2941 ttactattgg tgtttcgatt tttgttttag gggttattag tctctttttc aaagaactat
     3001 tattaacatc atttgatcct atcttagcca aatcaatggg tgtgaaggtc aatgcgtatc
     3061 attatttgtt gatggtccta ttaactttag tggctgttac ggcgatgcaa agcgtgggta
     3121 ctatcttgat tgttgcctta ctgattacgc cagcagcgac agcttattta tatgctaata
     3181 gcttaaaagt aatgttagtg atgtcatctt tactaggcgc tttagcatcg gttttaggac
     3241 tttatctggg ctataccttt aatgttgccg cagggtcaag tatcgtactg acttctgcga
     3301 tgatgttttt gatcagtttc tttgtttcac ccaagcaagg ctacctcaaa agatggatgc
     3361 aaaaaaagga aaaaacacct taaacttttc actttaaaca ccttagtggt ttgcaaatag
     3421 tggcaaatga catcaaaggg tgttttttat atgtcatcaa aagaccataa aaaaccttat
     3481 caaaccaagg tatttagcct attatctgat aaggtttttt taagcatgac ttagttttta
     3541 aagcgataat ctttgacaat gtcaattgag gtaatcgtaa tgtcttgttt tggtttatca
     3601 ttttggttga tagaagtcgc ggcaatttta tcgacaacat ccataccatc aatgacttga
     3661 ccaaatacag tataaccgcc atctaagctt ggattgccgc catgctcata ggcagagatg
     3721 ataggttttg ggtagttggt acttgataat cccttgcttt gatttttctt gttttggttg
     3781 atataaaatt ggctaccgtt agtattagcg ccagcatttg ccatggcaag agcaccgcga
     3841 atatgatata aaaatggaga gatttcgttg acaaagccat tgccagcatc ttttttagga
     3901 tccttgccct tccaaatcga ttcaccacct gtgccatctc ctttggggtc acctgattga
     3961 atcataaagt cgttgatcac acgatggaag gtaaggttat catagtagcc ttttttagca
     4021 tgggtgagga agttctcaac agctaaggga gcatattttg ggaataactt aagggtgatg
     4081 tccccttgac ttgtcctcat tacgacctta gcttcttctt tgccgacttc tttggttagt
     4141 tgtggaaatt ggctagcatc agctttgaga gctttttgaa tgctttcttc ataggcttta
     4201 gaagctgctt cgctttcttc tttggcagtt ttttcatcaa tgtatttatc gccttttata
     4261 gcgcggtcaa cagattcaca agcactcaaa ctaagtaaac taatggctac taaactgaga
     4321 gataataatt ttttcatgtc tactcctaag aatgattttc tttattttat cataaaatgc
     4381 aacagaaaat agtagtaaag agttaaaaac gcctattttt gtgttttatg atctaacctt
     4441 ggttaaaagg ctgctacgac tgttattata gcacaagatg ctatagaaaa acttaaaaca
     4501 ccttggtttt ttagagctat ttcctaatag ttaaaaaatg gtataatagt gttatggtta
     4561 aaagaaatca aagaaaaaaa agtgcgccta agaagcgttt aacaaaagca gaagtggaaa
     4621 agcaacgtgc cattaagagg atgattttat ctgttttaat ggctttgttg cttatttttg
     4681 cgatgcttcg cttgggcgtc tttggcgtga caacttataa tatgattcgt tttttggtgg
     4741 gtagtttagc ctatccgttt atgtttgcct ggttaattta cttattttgt tttaaatggt
     4801 tacggcaaaa agatggaatg attgcgggag ttgttattgc ctttttggga ttgctagttg
     4861 agtggcacgc cttcttattt gcgatgccta ggatgcttga tcaagctatt tttctaggca
     4921 ctgctcggct aattacaaga gatttactag ccctgcgcgt gactgaattt gttggtggag
     4981 gaatgcttgg tgcattactt tataaaccca tagccttttt attttcgaat atcggctctt
     5041 actttattgg ttttttgttt atcttattag gattatttct aatgacgcct tgggatatct
     5101 atgatgtgag tcactttgtg aaagaggctg ttgataaact agcagtggct tatcaggaaa
     5161 ataaagaaaa gcggttcatc aaacgagaag agcaccgctt acaagctgaa aaagaggctt
     5221 tagagaaaca agcacaagag gaagaaaaac gtctagcaga attgacggtt gatcctgaga
     5281 cgggagagat tgttgaggat agtcagtctc aagtcagcta tgatttggct gaggatatga
     5341 caaaagagcc agagattctt gcttatgata gtcacctcaa agacgatgag gcaagcttat
     5401 ttgaccaaga agacttggct tatgctcatg aggaaattgg ggcttatgac tctttatctg
     5461 ctttagcatc ttctgaagat gagatggata tggatgaacc tgttgaagtt gatttcacac
     5521 caaaaaccca tcttctttat aaacttccaa ctattgattt atttgcacca gataagccca
     5581 aaaaccagtc caaagagaaa aacttagtcc gcaaaaatat caaggtgctt gaagatacct
     5641 tccaaagttt tgggattgat gtcaaggtag aacgagcaga aatcgggcct tccgtcacca
     5701 aatatgaaat taaaccagct gtcggggtcc gagttaatcg tatttcaaat cttgctgatg
     5761 atttggcact tgctctggct gctaaagatg ttcgtattga agcaccaatt cctggaaaat
     5821 cgttaatagg tattgaagtt cctaactcag aaattgcaac ggtttctttt cgcgaacttt
     5881 gggaacaatc tgatgccaat cctgaaaacc ttttagaagt accactaggt aaagctgtta
     5941 atggcaacgc tcgcagtttt aacttagcta gaatgccgca tcttttggta gctggttcaa
     6001 ctggttcagg taaatctgtg gcagttaatg gcattatttc aagtattttg atgaaggcac
     6061 gtccagatca agttaagttt atgatgattg accccaaaat ggttgaatta tctgtttata
     6121 atgatattcc acatttatta atccctgttg taaccaatcc gcgtaaagcc agtaaggcac
     6181 tccaaaaagt ggttgatgag atggaaaacc gctacgagtt atttagtaaa attggtgtgc
     6241 gtaatatagc aggttataat acaaaggttg aagagtttaa tgcttcttct gagcaaaaac
     6301 aaataccgtt gcctttaatc gttgtcattg tagatgaatt ggctgacttg atgatggtgg
     6361 ctagtaaaga agttgaagat gctattattc gtttgggaca aaaagcacgt gctgcaggta
     6421 tccatatgat tcttgcaact caacgtccct ctgtagatgt tatttctggt ttgattaaag
     6481 caaatgttcc gtcgcgtatg gcatttgctg tttcaagtgg tactgatagc cgtacaatcc
     6541 ttgatgaaaa tggtgctgaa aagctcttgg gacggggtga tatgctcttt aagccgattg
     6601 atgaaaatca ccctgttcgc ttgcaagggt ctttcatctc agatgatgat gtggagcgta
     6661 ttgtcaactt tatcaaagac caagctgaag cggattatga tgacgccttt gatcctggtg
     6721 aagttagcga caatgatcct ggtttttctg gaaatggcgg agctgctgaa ggtgatcctc
     6781 tttttgaaga agcaaaagcc ttggtcttag aaacacaaaa agctagtgcc tcaatgattc
     6841 aaaggcgttt gtcggttgga tttaatcgtg ccacgcgctt gatggatgaa ttggaagaag
     6901 caggtgttat cggtccagca gaaggaacca aacctcgtaa ggtattgcag acaaactaaa
     6961 agaacctgac gtctcatgat gagatgttag gttctttttt tgtgatgtca ggccagttga
     7021 tgagatgcct atgctaatgt aaaaataaag ataactagaa taaggtagaa aataacggtg
     7081 actaaaaagc caactcgcca aaatagcttc ataaagcgat ggtaagaaaa cgactgagtt
     7141 cggatgacta gcagtagtga aatgacgatg gctagcagcg acatgataat aaggtaataa
     7201 ggcagtaaat tatgggtgaa aaattttcct gagacaagca caatttctat cgcaaaaagt
     7261 aaaagtgcga catctggaaa tttaaatccc tttttattaa gtttaaataa agaggttaca
     7321 atcatggcaa aaagaggcgt tactatcaag aaagctaagg cagttaattt atagatcatc
     7381 ataggtctat tttatactca aatcccttac ttgtaaactg atacttacct gttgtgatag
     7441 aaaatgatct ttgagaggga aagcgcctac ttcaatttaa acattgcaaa aggcatcagc
     7501 tattttaaca aaaagacttg atttattttg gcaataagct ataatagtaa ggtatgcaat
     7561 attgcatatc tgtgggaggt aaaaatctca attaccgcca aaaccacaac aggaggattt
     7621 ttaaaatggc taaaaaagtc gaaaaacttg taaaacttca aatccctgct ggtaaagcta
     7681 caccagctcc accagttgga ccagctcttg gtcaagcagg tatcaacatc atgggcttca
     7741 ctaaagaatt taacgctcgt acagctgatc aagctggtat gatcatccca gttgttatct
     7801 cagtttatga agacaaatca tttgatttca tcactaaaac accaccagct gctgttcttt
     7861 tgaaaaaagc tgcaggtgtt gaaaaaggat caggtacacc taacactact aaggttgcga
     7921 cagttactcg tgcacaagta caagaaattg ctgaaactaa gatgccagat ttgaacgctg
     7981 caaacattga agctgcaatg cgtatgatcg aaggtactgc tcgttctatg ggattcactg
     8041 ttactgacta gtcagtcaag aacccctatt acccgcacga cttcatcata agagatgagt
     8101 gacgtgggag attttaaatc aaatcgatat gaccacatta caaggagaat tttaaaatgg
     8161 ctaaaaaaag caaacaaatg cgtgctgcac ttgaaaaagt agatagcaca aaagcgtaca
     8221 gtgtagaaga agctgtagca ttagtaaaag aaacgaactt cgcaaaattt gatgcgtctg
     8281 ttgaagttgc ttacaacttg aacattgacg ttcgtaaagc agaccaacaa atccgtggcg
     8341 caatggtatt gccaaacgga actggtaaaa cacaacgtgt tcttgttttt gcacgtggtg
     8401 caaaagctga agaagcaaaa gcagctggtg cagacttcgt aggtgaagac gaccttgttg
     8461 caaaaatcaa tggtggatgg cttgactttg acgttgttat cgcaacgcca gacatgatgg
     8521 ctatcgtagg tcgtcttgga cgtgtccttg gacctcgtaa cttgatgcca aaccctaaaa
     8581 ctggtacagt aacgatggat gttgctaaag ccgttgaaga gtctaaaggt ggtaaaatca
     8641 cttaccgtgc tgacaaagca ggtaatgttc aagctcttat tggtaaagtt tcatttgatg
     8701 ctgacaaatt ggttgaaaac ttcaaagcct tccacgatgt aatggctaaa gctaaacctg
     8761 caacagctaa aggaacttac atggcaaacg tctcaatcac atcaacacaa ggtgttggta
     8821 tcaaggttga tcctaactca ctttaagaaa aaaattaggg catcgcctaa tcatagcaaa
     8881 aagacaaatt tctgaagaca aatcttcact agaggtttgt ctttttttat ggtttaaaat
     8941 ggaatgcaaa caaaaaagtg taggcgataa taccatgaaa ggagctgatt ctcttaatga
     9001 gatgtggtat taacacatca tgagctcaca aaaaatgata aaggaagtat cggtggtgtt
     9061 aaaaaaggga ttttaattga tatttattag cctctaaaat gtcctttccc tattcataat
     9121 tgagggtttg tggtaaaata gtaaggacaa actaaggaga acatagtagt ggaacctaaa
     9181 tatcaacgta tattaattaa gttatctggt gaagcattag caggtgaaaa aggagttggc
     9241 attgacattc caactgttca ggctattgca aaagaaattg ccgaagttca tgtgtcggga
     9301 gtacagatcg ctcttgttat tggtggaggc aatctctggc gtggagagcc tgcagcagac
     9361 gctggaatgg accgtgttca ggctgattac actggcatgc ttggaacggt gatgaatgcc
     9421 ctagtcatgg ctgatagtct tcaacattat ggtgttgata cccgtgtgca aacagctatt
     9481 cctatgcaaa atgtagcaga accttatatt cgcggacgtg ccttgcgcca tctagaaaaa
     9541 aatcggattg ttgtttttgg agctggtatt ggctcgcctt atttttcaac agacaccacc
     9601 gcggcactgc gtgctgctga gattgaagca gatgctattt taatggcaaa aaatggtgtg
     9661 gatggggtct ataatgctga tcctaaaaaa gatgctaatg cggttaaatt tgatgaatta
     9721 acacatggtg aagtgatcaa acgtggtctc aaaatcatgg atgcaacggc atcgacctta
     9781 tcgatggaca acgatattga tctggtggtt ttcaatatga atgaagctgg caatattcaa
     9841 cgtgttgtct ttggagaaca tattggaact actgtatcaa ataaagtttg tgactaaaag
     9901 ttagaaaaga aagagagtaa aaaaacatgg caaatgcaat tattgaaact gcaaaagaac
     9961 gttttgcaca atcccatcag tctttatcac gtgaatatgc cagcattcgt gcgggtcgtg
    10021 ccaacgctag tcttttagac cgtatccaag ttgattatta cggggcaccg acgccattaa
    10081 atcaattggc ttcaatcact gtgccagaag cgcgtgtctt attgatctcg ccttttgata
    10141 aatcttctat caaagatatc gagcgcgctc ttaacgcatc agatttaggc attacacctg
    10201 ctaacgatgg ctctgtcatt cgtttggtta ttccagcttt gaccgaagaa acacgtaaag
    10261 aattagccaa agaagtgaaa aaagttggtg aaaatgctaa aattgcaatc cgtaatatcc
    10321 gccgtgatgc tatggatgat gctaaaaagc aagaaaaagc aaaagaaatc actgaagatg
    10381 agttaaaaac acttgagaaa gatatccaaa aagcaaccga tgatgctatc aaagaaattg
    10441 accgcatgac ggctgaaaaa gaaaaagaat tgctctcagt gtaacaggat ttctacgaaa
    10501 aatagacgac tatagacaca aaaagatagg tgaccatttc agaagcgttt ggttttgatt
    10561 tgtccctatt ttttaaagaa agaaaaaaga tatgaatgat ttactagcaa cagtcattac
    10621 tgggcttatt aaagaagaaa acgctaatga ctattttatc caaaaagagg ggtttacctt
    10681 taccttgtct aaagcagaag gagaacgcca gattggtgat atggtcacag gctttgccta
    10741 tacagacata aagcaaaagg cacgtctcac aaccaaagag atccgttcaa cacggacaag
    10801 ttatggctgg ggggaagtga cagaggttcg tcgtgaccta ggcgtttttg tcgatacagg
    10861 aattccaaat aaagaaattg tggtttcttt ggacgtgttg cctgaaatga aagaactatg
    10921 gcctaaaaaa ggggataaac tttacattcg cttagaagtg gataaaaaag accgtatttg
    10981 gggacttcca gcagagcctg aggtctttca aaagatggct agccctgcat acaataacat
    11041 gcaaaatcaa cattggccag caattgttta ccgcctaaaa ctaacaggaa cgtttgttta
    11101 cctgcctgaa aacaacatgt tgggctttat tcacccaagt gaacgctacg cagaaccgcg
    11161 cttaggtcag gttttagatg cccgtgtgat tggctttaga gaggtcgatc gtaccctaaa
    11221 cttgtcgttg aaaccacgtt cttttgagat gttggagaat gacgctcaga tgattgttac
    11281 ttatctggaa gccaatggtg gtttcatgac cttaaatgac aagtcaagtc cagaagagat
    11341 taaggcaacc tttggtattt caaaaggcca gtttaaaaaa gccttgggtg gattgatgaa
    11401 agctaaacgc atcaaacaag acgccacagg aactgaatta ataggataaa aatgctttgc
    11461 cactggcaaa gttttttgtt acaatgaaga ccattaactg aagaaaaagg ggatatagag
    11521 aatggaaaga gccatttttg caggaggttg tttttggtgt atggtgcaac cttttgagga
    11581 gcaagcaggt attttgtcag tcagaagtgg ctacacaggt gggcatctcc caaatcctag
    11641 ctatgaacaa gtttgtgcca aaacaacagg acatacagaa gctgttgaaa ttatttttga
    11701 ccctaagcaa atcgcttata aagatttggt agagctctat tgggcgcaga cggatccaac
    11761 agacgccttt gggcaatttg aagatcgtgg ggacaactac cgtcctgtca tttactacac
    11821 aacggaaaga caaaaagaaa ttgcagagca atcaaaagca aacttgcaag cttcaggacg
    11881 ttttgaccaa cccattgtca ccactattga accagcagag ccattttatc tcgcagagga
    11941 ttatcaccaa ggtttctata aaaagaatcc caagcggtat gcacaaagca gtgctattcg
    12001 tcatcagttt ttagaggaga attggtcatg agaaaatcat tttacagctg gctgatgaca
    12061 caacgtaacc ctaaatcaaa tgaacctgtg gcgattttag cagatttggt gtttgatgac
    12121 accacttttc caaagcatac caatgatttt gaactcatta gtcggtatct agaagatcaa
    12181 gctagttttt cctttaattt aggtcaattt gatgaaatct gggaagatta tctagcacat
    12241 taatacgtgg ataaaaaaga gtcatatcga ctcttttgaa gggttatcac gttattatta
    12301 caaaatgaca tcactttctt tcaaaaaaat gacaagctac tttttttctt aaaaatatgc
    12361 tatgatatag cttagaaaaa aataaaaagg aagcagagat gattattact aaaaagagct
    12421 tatttgtgac aagtgtcgct ttgtcgttag cacctttggc gacagcgcag gcacaagagt
    12481 ggacaccacg atcggttaca gaaatcaagt ctgaactcgt cctagttgat aatgttttta
    12541 cttatactgt aaaatacggt gacactttaa gcacaattgc tgaagcaatg gggattgatg
    12601 tgcatgtctt aggagatatt aatcatattg ctaatattga cttaattttt ccagacacga
    12661 tcctaacagc caactacaac caacacggtc aggcaacgac tttgacggtt caagcgcctg
    12721 cttctagtcc agctagcgtt agtcatgtac ctagcagtga gccattaccc caagcatctg
    12781 ccacctctca atcgactgtt cctatggcac catctgcgac accatctgat gtcccaacga
    12841 caccattcgc atctgcaaag ccagatagtt ctgtgacagc gtcatatgag ctcacatcgt
    12901 caacgaatga tgtttcgact gagttgtcta gcgaatcaca aaagcagcca gaagtaccac
    12961 aagaagcagt tccaactcct aaagcagctg aaacgactga agtcgaacct aagacagaca
    13021 tctcagaaga ttcaacttca gctaataggc ctgtacctaa cgagagtgct tcagaagaag
    13081 tttcttctgc ggccccagca caagctccag cagaaaaaga a
//
LOCUS       DAUCLR010000051        13113 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.051, whole genome shotgun sequence.
ACCESSION   DAUCLR010000051 DAUCLR010000000
VERSION     DAUCLR010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13113
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.051"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            92..1462
                     /locus_tag="VGT87_001213"
     CDS             92..1462
                     /locus_tag="VGT87_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEQ3814067.1"
                     /translation="MSKIVVVGANHAGTACIKTMLTNYGDANEIVVFDQNSNISFLGC
                     GMALWIGEQIAGPEGLFYSDKEELESLGAKVYMESPVQSIDYDAKTVTALVDGKNHVE
                     TYDKLIFATGSQPILPPIKGAEIKEGSLEFEATLENLQFVKLYQNSADVIAKLENKDI
                     KRVAVVGAGYIGVELAEAFQRKGKEVVLIDVVDTCLAGYYDRDLTDLMAKNMEEHGIQ
                     LAFGETVKEVAGNGKVEKIITDKNEYDVDMVILAVGFRPNTTLGNGKIDLFRNGAFLV
                     NKRQETSIPGVYAIGDCATIYDNATRDTNYIALASNAVRTGIVAAHNACGTDLEGIGV
                     QGSNGISIYGLHMVSTGLTLEKAKRLGFDAAVTEYTDNQKPEFIEHGNFPVTIKIVYD
                     KDSRRILGAQMAAREDVSMGIHMFSLAIQEGVTIEKLALTDIFFLPHFNKPYNYITMA
                     ALGAKD"
     gene            complement(1621..2604)
                     /locus_tag="VGT87_001214"
     CDS             complement(1621..2604)
                     /locus_tag="VGT87_001214"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEQ3814068.1"
                     /translation="MTATKQHKKVILVGDGAVGSSYAFALVTQNIAQELGIIDIFKEK
                     TQGDAEDLSHALAFTSPKKIYAADYSDCHDADLVVLTAGAPQKPGETRLDLVEKNLRI
                     NKEVVTQIVASGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQAL
                     AAKIGVDARSVHAYIMGEHGDSEFAVWSHANVAGVGLYDWLQANRDIDEQGLVDLFIS
                     VRDAAYSIINKKGATFYGIAVALARITKAILDDENAVLPLSVFQEGQYEGVEDCYIGQ
                     PAIVGAYGIVRPVNIPLNDAELQKMQASANQLKAIIDEAFAKEEFASAAKN"
     gene            2795..5281
                     /gene="gyrA"
                     /locus_tag="VGT87_001215"
     CDS             2795..5281
                     /gene="gyrA"
                     /locus_tag="VGT87_001215"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEQ3814069.1"
                     /translation="MQDRNLIDVNLTSEMKTSFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRHMLVD
                     GHGNFGSMDGDGAAAQRYTEARMSKIALELLRDINKNTVNFQDNYDGSEREPVVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLAESIDAVKMVMEHPDCTTRELMEVIPGPDFPTG
                     ALVMGRSGIHRAYDTGKGSIVLRSRTEIETTQTGRERIVVTEFPYGVNKTKVHEHIVR
                     LAQEKRLEGITAVRDESSREGVRFVIEIRREASATVILNNLFKLTSLQTNFSFNMLAI
                     ENGVPKILSLRQIIDNYISHQKEVIIRRTRFDKDKAEARAHILEGLLIALDHLDEVIA
                     IIRNSETDVIAQTELMSRFDLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LSDILAKPERIITIIKEEMDEIKRKYANPRRTELMVGEVLSLEDEDLIEEEDVLITLS
                     NKGYIKRLAQDEFRAQKRGGRGVQGTGVNNDDFVRELVSTSTHDTLLFFTNFGRVYRL
                     KAYEIPEYGRTAKGLPIVNLLKLEDGETIQTIINARKEETAGKSFFFTTKQGIVKRTE
                     VSEFNNIRQNGLRALKLKEGDQLINVLLTSGQDDIIIGTHSGYSVRFNEASIRNMGRS
                     ATGVRGVKLREDDRVVGASRIQDNQEVLVITENGFGKRTSATDYPTKGRGGKGIKTAN
                     ITPKNGQLAGLVTVDGTEDIMVITNKGVIIRTNVANISQTGRATLGVKIMKLDADAKI
                     VTFTLVQPEDSSIDEINTDRENSISKNKDN"
     gene            5301..6050
                     /locus_tag="VGT87_001216"
     CDS             5301..6050
                     /locus_tag="VGT87_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEQ3814070.1"
                     /translation="MVKKQKRRKIKSMSWARKLLIAVLLILGLALLFNKPIRNTLIAR
                     NSNKYQVTKVSKKQIKKNKEAKSTFDFQAVEPVSTESVLQAQMAAQQLPVIGGIAIPE
                     LGINLPIFKGLGNTELIYGAGTMKEEQVMGGENNYSLASHHIFGITGSSQMLFSPLER
                     AQNGMSIYLTDKEKIYEYIIKDVFTVAPERVDVIDDTAGLKEVTLVTCTDIEATERII
                     VKGELKTEYDFDKAPADVLKAFNHSYNQVST"
     gene            6134..6547
                     /locus_tag="VGT87_001217"
     CDS             6134..6547
                     /locus_tag="VGT87_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEQ3814071.1"
                     /translation="MKLNAIHHVAIIVSDYNLSKNFYVNKLGFEIIRENYRPDKHDYK
                     LDLSCGRIELEIFGKVTSDPYYQAPPKRVSQPEFKSEACGLRHLAFRVTNIESYVDDL
                     KSLGILVEPIRHDDYTGEKMTFFFDPDGLPLELHE"
     gene            7161..7535
                     /locus_tag="VGT87_001218"
     CDS             7161..7535
                     /locus_tag="VGT87_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814072.1"
                     /translation="MTQKNHGVFWGLLLGSAAASIAYLSLSSSKKDQLLKDSAKKIDD
                     LNAYLQDKSKQVLDAVSEKVQESKDAVEVYGGIAAETVEESLGQAKEKVEGIGEATSQ
                     TIQSKMEKLTSDKTDAADDDEK"
     gene            complement(7585..8496)
                     /locus_tag="VGT87_001219"
     CDS             complement(7585..8496)
                     /locus_tag="VGT87_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEQ3814073.1"
                     /translation="MKTVRIFYNPNSGKKEDQLAGQVKDYFCQHGFSEDSVEVITPKN
                     ADQAFQLAKQAAKDKIDLVIPLGGDGTLNKIIGGIYEGGAHCLIGLVPSGTVNNFAKA
                     MHIPLQITEALDTILTGQIKQVDICKANQQYMISSLTLGLLADIAADVTAEEKRRFGP
                     LAFLKDSIRILKRNRSYAISLISHNHRIHLKTKFLLITMTNTIAGFPSFSPGAQADDG
                     YFQVYTMKKVSFFKFLWHINDFKQGDFSKAEEISHFQANTLSLLPQAKKQAILPRTRI
                     DGDKSDYLPIQLDIIPKAISIIVPTED"
     gene            complement(8613..9263)
                     /locus_tag="VGT87_001220"
     CDS             complement(8613..9263)
                     /locus_tag="VGT87_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEQ3814074.1"
                     /translation="MNQTFKQSLPLSFSEEVANSVTHAIGAFAMLILLPISASYAYQT
                     YDLKAAIGISIFVISLFLMFLSSTIYHSMAYGSVHKYILRIIDHSMIYIAIAGSYTPV
                     ALSLVSGWLGYIIIVLQWGITLFGILYKIFAKRINEKFSLMLYIVMGWLVVFILPVII
                     QKTSLAFGLLMLFGGLSYTIGAVFYAKKRPYFHMIWHLFILLASALQFIAITFFML"
     gene            complement(9260..9700)
                     /locus_tag="VGT87_001221"
     CDS             complement(9260..9700)
                     /locus_tag="VGT87_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEQ3814075.1"
                     /translation="MIFPSLPYWKDLPDLDLYLDQVLLYVNQCTDFSEVSDNKSLTAS
                     MINNYVKHGYVTKPIKKKYQKQQLARLIAISLFKTVFPIQDISRVLEELQAQADSESL
                     YNTFVTCWNQKAPIEEDIPPIVQVACQTVKDYHKTIYLSQEVTQ"
     gene            9919..10767
                     /gene="ylqF"
                     /locus_tag="VGT87_001222"
     CDS             9919..10767
                     /gene="ylqF"
                     /locus_tag="VGT87_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEQ3814076.1"
                     /translation="MAMIQWFPGHMSKARRQVQENVKHVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLMILNKADLADATRTKEWKAYYESQGIKTLAINSKEQSTVKKVTEAAK
                     ELMADKIQRLRERGIQKETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKSNKELEILDTPGILWPKFEDELVGLKLALTGAIKDQLLPMDEVTIFGLNYFREYY
                     PNRLTKRFKNIPLEEEAPEIIMTLTRQLGFKDDYDRFYTLFVKEVRDGKLGQYTLDQV
                     GDMDAD"
     gene            10757..11548
                     /locus_tag="VGT87_001223"
     CDS             10757..11548
                     /locus_tag="VGT87_001223"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEQ3814077.1"
                     /translation="MPTSIKAIKESLEAVTSLLDPLFQELATDTRLGVQKALKSRQKA
                     IQAELAEEERLEAMLSYEKALYKKGYQAIAGIDEVGRGPLAGPVVAACVILPKYCKIK
                     GLNDSKKIPKAKHETIYQAVKEKALAIGIGIIDNQLIDEVNIYEATKLAMLEAIKQLE
                     GQLTQPDYLLIDAMTLDIAISQQSILKGDANSLSIAAASIVAKVTRDQMMANYDRIFP
                     GYGFAKNAGYGTKEHLQGLKAYGITPIHRKSFEPVKSMCCDSTNP"
     gene            11613..12449
                     /gene="dprA"
                     /locus_tag="VGT87_001224"
     CDS             11613..12449
                     /gene="dprA"
                     /locus_tag="VGT87_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEQ3814078.1"
                     /translation="MNHFELYKLKKAGLTNKNILNILDYQKQQEKSLSLRDMAVVSGC
                     KHPSHFIEAYKQLDIQKLKMEFKQFPSISILDKHYPMALKEIYNPPVLLFFQGNLDLL
                     EKPKLAIVGSRRSSDTGVKSVRKILKELGNRFVIISGLARGIDTSAHLACLKNGGQTI
                     AIIGTGLDRFYPKENRELQTFLGKNHLVITEYGPGEEALSYHFPERNRIIAGLSRGIL
                     VVEAKNRSGSLITCQIGIEEGRDIFAVPGNILDGKSEGCLQLIKEGATCVTSGMDILS
                     EY"
     gene            12555..>13113
                     /locus_tag="VGT87_001225"
     CDS             12555..>13113
                     /locus_tag="VGT87_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HEQ3814079.1"
                     /translation="MGTKTTTKPKTGTKKSTTKKKSTAKKNLVIVESPAKAKTIEKYL
                     GRSYKVVASVGHIRDLKKSSMSIDFDNNYEPQYINIRGKGPLINSLKKEAKAAKKVYL
                     ASDPDREGEAISWHLSHILGLDPQDNNRVVFNEITKDAVKHAFVEPRQIDMDLVDSQQ
                     ARRVLDRIVGYSISPILWKKVKKGLS"
BASE COUNT         4302 a         2265 c         2673 g         3873 t
ORIGIN      
        1 actcaaaaaa gtgagcaata tatttggaag cgttttttga ctgtggtata atagagccct
       61 atataatatt gtgaaatgag gaacaaaata tatgagtaaa atcgttgttg ttggtgcaaa
      121 ccatgctggt acggcctgta tcaagactat gttaacaaac tacggtgatg ctaatgagat
      181 tgttgtattt gaccaaaact caaatatttc atttttaggc tgtggtatgg cactttggat
      241 tggtgagcaa attgctggac cagaaggact tttctattca gataaagaag aattagagtc
      301 tttaggggct aaagtttaca tggaatcacc tgttcaatca atcgactacg atgccaaaac
      361 agtgacagcg cttgttgatg gtaaaaacca cgtggaaact tacgacaagt tgatttttgc
      421 aactggctca caacctatct taccaccgat taaaggtgcc gaaatcaaag aaggatcact
      481 tgaatttgaa gcaactcttg aaaatcttca atttgtgaag ttataccaaa actcagctga
      541 tgtgattgca aagcttgaaa acaaagacat taaacgtgta gccgtagttg gtgctggtta
      601 cattggtgtt gagttagctg aagctttcca acgcaaaggc aaagaagtgg ttctgattga
      661 tgtcgtggac acttgcttgg caggttatta cgaccgtgat ttgactgact taatggctaa
      721 aaacatggaa gaacatggta ttcaactagc ctttggtgaa acagttaaag aagtagctgg
      781 taatggtaag gttgaaaaga tcattactga caaaaatgag tacgatgtgg atatggttat
      841 cctcgccgtt ggtttccgtc caaacacaac tcttggaaat ggtaagattg atcttttccg
      901 taatggtgct ttccttgtca ataaacgcca agaaacttct attccaggtg tttatgctat
      961 cggtgactgt gcaactatct acgataatgc tactcgcgat acaaactaca ttgctttagc
     1021 ttcaaatgct gtccgtacag gaattgtagc agcacataat gcttgtggta cagaccttga
     1081 aggaatcggt gtccaaggct caaacggtat ttccatttac ggcttgcaca tggtatcaac
     1141 tggtttgact cttgaaaaag ctaaacgact tggttttgat gctgcagtga ctgagtatac
     1201 tgataaccaa aaacctgaat ttatcgaaca cggtaacttc ccagtaacca ttaagattgt
     1261 ttacgataaa gactcacgtc gtatcttggg tgctcaaatg gcagcccgtg aagatgtgtc
     1321 aatgggaatt cacatgttct cacttgctat ccaagaaggc gtaaccattg aaaagttggc
     1381 attaactgat attttcttct taccacattt caacaaacct tacaactaca tcacaatggc
     1441 agcacttggt gccaaagact agtatctgat aggaaaaagc cagagcagtt cagcagcact
     1501 ggcttttttg ctttttaatt gttgcaacct gaaaagttct aaacttatga ttatcctctt
     1561 atagcacgtt ggaaaaggaa tatccggcaa aacaaaaaga gaccaaagtc tcttttgtga
     1621 ttagttttta gcagcagaag caaattcttc cttagcaaag gcttcatcaa tgatagcttt
     1681 taattgattt gcagaggctt gcattttttg caattcagca tcgttaagtg gaatgtttac
     1741 tggacgaaca attccatacg ctccaacgat agctggttga ccaatatagc aatcttcaac
     1801 accttcgtat tggccttctt ggaaaacaga aagtggaaga acagcatttt catcatcaag
     1861 aatagctttt gtaatacgag ctagggcaac tgcaataccg tagaaagtag cacctttttt
     1921 attgatgatt gagtaagcag catcacgtac agaaatgaaa agatcaacta ggccttgctc
     1981 atcaatgtca cgattagctt ggagccagtc gtaaagacct acaccagcaa cgttagcgtg
     2041 tgaccaaaca gcaaattcag agtcaccgtg ttctcccatg atgtaagcgt gaaccgaacg
     2101 cgcatcaaca ccgattttag cagccagagc ttgacggaaa cgggctgaat ctagagatgt
     2161 acctgatcca ataacacgct ctttagggaa acctgagaat ttccatgttg agtaagtcaa
     2221 aacgtcaact gggttagcag caacaagaaa gattcctttg aaaccagatg caacgatttg
     2281 tgtaacaacc tctttattga tacgaaggtt tttttcaaca aggtcaaggc gtgtttcacc
     2341 tggtttttga ggagcaccag ctgttaagac aacaagatca gcgtcatgac agtctgagta
     2401 gtcagctgca tagatttttt taggtgatgt aaaagctaga gcgtggctta aatcttctgc
     2461 atctccttga gttttttctt taaaaatatc aataatacca agttcttgag caatattttg
     2521 tgttaccaat gcaaaagcgt atgatgaacc gacggcacca tcaccgacca agattacttt
     2581 tttgtgttgt ttagttgcag tcatttctaa acatctcctt aattttatta gagcaaaagc
     2641 tcatacggtc ttattttaac atattgataa ttgtttgtca ttgaattcag aaaaataatg
     2701 aaaatagttt cctgataacg cttccaataa gaaaaaataa ggaatttatg gtataatgaa
     2761 atctagtatt gactagaaaa ggagcattcc ttaaatgcaa gatcgaaatt taattgacgt
     2821 caatctgaca agtgaaatga aaacgagttt cattgactat gccatgagtg tcattgtggc
     2881 aagagctttg ccagatgtgc gtgatggtct gaaacctgtg catcgtcgta ttctctatgg
     2941 gatgaatgaa ctcggtgtga cacctgataa accgcataaa aaatctgctc gtattacggg
     3001 tgatgtcatg ggtaaatatc acccgcatgg ggattcttct atttatgaag ctatggttcg
     3061 tatggctcag tggtggagtt atcgccatat gcttgttgat ggccatggca attttggttc
     3121 tatggatggt gatggtgctg ccgctcaacg ttatactgaa gcgcgcatga gtaaaattgc
     3181 gcttgaatta ttaagggata tcaataaaaa taccgttaat tttcaagata actacgatgg
     3241 aagcgaaaga gaaccagtag tattaccagc tcgctttcct aatttattag tcaatggagc
     3301 gacagggatt gccgttggga tggcaactaa cattccgcct cataatttgg cagagtctat
     3361 tgatgctgtc aagatggtaa tggaacatcc agactgtaca acaagagagt tgatggaagt
     3421 gatccctgga cctgactttc cgactggtgc ccttgttatg gggcgctcag gtattcatag
     3481 ggcttatgat actggtaaag gttctatcgt tttacgctct cgaacagaaa ttgaaacaac
     3541 tcaaacaggt cgggaacgta ttgtggttac tgaatttccc tatggcgtca ataaaacgaa
     3601 agtgcatgaa cacattgttc gtttagcaca agaaaaaaga ctagaaggta ttacagcagt
     3661 tcgtgacgaa tctagccgtg aaggagtgcg ttttgttatc gagattcgtc gagaggcatc
     3721 cgcgacagtt atcttaaata acctctttaa attaacaagc cttcagacga atttcagttt
     3781 taatatgttg gctattgaaa atggtgtgcc taaaatattg tcactcagac aaatcattga
     3841 taattacatt agtcatcaaa aagaagttat tatacgtcgc acccgatttg ataaagataa
     3901 agctgaagcc agagctcata ttttagaagg cttactgatt gcccttgatc atttagacga
     3961 agtgattgct attattcgta acagcgagac agatgtcatt gctcagacag aattgatgtc
     4021 acgtttcgac ttgtctgaac gccaaagtca agctattttg gatatgcgtt tgcgtcgttt
     4081 gactggtttg gaacgtgata agattcaatc agaatatgat gatttgttgg ccttgattgc
     4141 tgacttatct gatattttag ccaaaccaga acgtattatc actatcatca aagaagaaat
     4201 ggatgagatc aagcgtaaat acgctaaccc acgccgtaca gaattaatgg tcggtgaagt
     4261 cctttctcta gaagatgaag acctaattga agaagaagat gttttgatta ccttgtcaaa
     4321 taaaggttat atcaagcgtc ttgctcaaga tgaatttcgt gcccagaagc gtggtggacg
     4381 tggcgtccaa ggaactgggg tcaacaatga cgactttgtc cgtgaattgg tatcaacaag
     4441 tacccatgat actttgctct tctttacgaa ttttgggcgt gtctataggt tgaaagctta
     4501 cgaaattcca gaatatggtc gtacagcaaa aggtcttcca attgttaatc tattgaaatt
     4561 agaagatggt gaaaccattc aaactattat caacgcacgt aaggaagaaa cagcaggtaa
     4621 atccttcttc tttacaacga aacaaggcat tgtcaaacgt acagaagttt cagaatttaa
     4681 taatatccgt caaaacggtc tccgtgctct taagttgaaa gagggagatc agctgattaa
     4741 cgtccttttg acaagcggtc aggatgacat tatcattgga actcattccg gatattctgt
     4801 tcgttttaat gaagcaagca tccgcaacat gggccgatca gctacgggtg ttcgcggggt
     4861 taagcttcgt gaagatgatc gcgttgttgg cgcgtcacgt attcaagata accaagaagt
     4921 tcttgtaatt accgaaaatg gctttggtaa gagaacaagt gctacagact atcctacaaa
     4981 aggtaggggg ggaaaaggaa taaaaacagc taatattacc cctaaaaatg gtcaattagc
     5041 tggattggtt accgtagatg gtacagaaga tatcatggtg attacaaata aaggtgtcat
     5101 tattcgcaca aatgttgcaa atatttcgca aacaggtcgt gctactttgg gtgttaaaat
     5161 tatgaaacta gatgctgatg ctaagattgt aacatttacc ttggttcagc ctgaagacag
     5221 tagtatcgat gaaatcaata ctgatcgtga aaatagtatt tcaaaaaata aagataatta
     5281 atcataacaa ggagtaaaaa atggtaaaaa aacaaaagcg tcgaaaaata aaatccatgt
     5341 cttgggcaag aaaactacta atagctgtac ttttaattct aggtttagca ttgcttttta
     5401 acaaacctat ccgaaataca ttaattgctc gaaactctaa taaatatcaa gtaacgaaag
     5461 tttcgaaaaa acaaatcaaa aagaataaag aggctaagtc aacatttgat ttccaagccg
     5521 tagagccagt tagtacagag agtgtcttgc aagcacaaat ggcggctcag caacttcctg
     5581 ttataggggg cattgccata ccagagcttg gcattaattt accaattttt aaaggtttag
     5641 gaaatactga gcttatttat ggcgcaggaa cgatgaaaga agaacaagtt atgggaggag
     5701 aaaataatta ttctcttgcc agtcatcata tttttggaat tacaggttca tctcaaatgc
     5761 tcttttcgcc gcttgaaaga gcacaaaatg ggatgtccat ctatttaaca gataaagaaa
     5821 aaatttacga atacatcata aaagatgttt tcacggtagc tcctgaacgc gttgatgtta
     5881 tcgatgatac agctggtctc aaagaagtga ctttagtgac ttgtacagat atcgaagcaa
     5941 cagaacgtat tattgtcaaa ggagaactaa aaacagaata cgattttgat aaagcgcccg
     6001 ccgatgtatt gaaagctttt aatcattctt ataaccaagt atctacctag atatatctta
     6061 aatataatat ttaaaaagta ggaccataga gaaaatttat ctcatcctac ttttttttga
     6121 aaggagaaat attatgaaac taaatgccat tcaccacgtt gctattattg tatctgatta
     6181 taatctgtca aaaaattttt atgtgaataa actcggattt gaaattatca gagaaaatta
     6241 tcgtcctgac aagcatgatt acaaattaga tttatcttgt ggtcgtattg aattggagat
     6301 ttttggtaag gtgacaagtg acccctacta tcaagctcct cctaaacgtg taagtcaacc
     6361 agaatttaaa agtgaggcct gtggtttgag gcaccttgct tttcgtgtta ccaacattga
     6421 gagttatgta gatgatctaa agagcttagg aattctagtg gagcctatca ggcatgatga
     6481 ttacacaggt gagaaaatga ctttcttttt tgatccggat ggtttgcctt tagaattaca
     6541 tgaatagact tattaggtta acgactggat cgtgctataa aactcgttaa aaatagttat
     6601 ttgttgtatc ttcatttgct tgattaatct cttttgatcg ttaaatgatt tggttaactt
     6661 tcaaagtgaa gagcaatttt tggagtaatc ttgtcgaagt tttttaatag aataactata
     6721 gaaaaagcaa aatatttgac gataaactct tacagtgtta taatgacaat ataagttaat
     6781 aactgtgttg tcactttgtt gaaatgtttt cgtattaatg tttttttatc tattacgata
     6841 gataatttta cttcaatttt tgattgaaaa tactaaaata agtgatgtgg tcactttgac
     6901 aaccatttat tggtcctttt agataagaca attttgtggc tcttcagttg aaaggatgtg
     6961 ttgttgatgt tagaggaaac aatcatgtta aattaactca ggcttaaaga ccaaaaaagg
     7021 agtgaagtct attatgaaaa aaagaaattt taaacttaca gaaatgttac aattggtttt
     7081 attagtggtt cctgctgtaa aacttgctaa aaaaatgttt aacgatagta aaacaccgtc
     7141 taaaagaaag taggtctaca atgactcaaa aaaatcatgg cgttttttgg gggttgttgc
     7201 ttggatcagc agcagctagc attgcttact taagtttatc gtcttccaaa aaagatcaac
     7261 tcttgaaaga tagtgctaaa aaaatagatg atctcaacgc ttacttgcaa gataaaagta
     7321 agcaagttct ggatgcagta tcggaaaaag tacaagaatc caaagatgct gttgaagttt
     7381 atggtggtat tgccgcagaa acagttgaag aatctttggg acaggctaaa gaaaaggtag
     7441 aaggaattgg cgaagctact agtcaaacta tccaatctaa aatggaaaaa ctcaccagcg
     7501 ataagacaga tgctgctgat gatgatgaaa aataggtttt aaaatacctt taagataaga
     7561 caaaaagtgc cattaggcac ttttttaatc ttctgttgga acaataatcg aaatggcttt
     7621 tggaatgata tctaattgaa tgggcagata gtcactttta tccccatcaa tcctggtacg
     7681 tggtaatatc gcttgttttt tagcttgtgg tagtaagcta agcgtattgg cttgaaaatg
     7741 actgatttcc tctgctttgg aaaaatcacc ctgtttaaaa tcattaatat gccataaaaa
     7801 tttaaagaaa gagacttttt tcatggtata aacttggaaa tagccatcgt cagcttgagc
     7861 accaggagaa aaagaaggaa aaccagcaat ggtatttgtc atcgtgatta agagaaactt
     7921 cgtttttagg tgaatacgat ggttgtggct aattaaagag atggcatagc tgcgatttcg
     7981 tttcagtatt cgaatactat cttttaaaaa tgctagagga ccaaagcgtc gtttttcctc
     8041 agccgttacg tcggcagcaa tatcagctag taatcctaag gttaaactgg aaatcatgta
     8101 ttgctggtta gctttgcaaa tatcaacttg tttgatttgt cccgtcaaga tagtatccaa
     8161 cgcctcagta atttgtaaag ggatatgcat agccttggca aaattattga ctgtccctga
     8221 gggcactaag cctattaaac aatgagctcc tccttcatag atgccaccga tgattttatt
     8281 tagtgtccca tcgccaccaa gagggataac caagtcaatc ttgtccttag cagcttgttt
     8341 tgctaattga aaagcctggt cagcattttt aggggtaata acttctacag aatcttcgga
     8401 aaaaccatgt tggcaaaaat agtctttgac ttgcccagca agttgatctt ctttctttcc
     8461 agaattagga ttgtaaaaaa tccgaacggt tttcatcagg tctctccttt gacagcttat
     8521 tacttgctat ccattatacc acttctaaga gttgatgtct tacatcaact catcacatta
     8581 agcctctcaa tcagtctgag catctatttg tcttaaagca taaagaaagt gatagcgata
     8641 aattggaggg cagaggccaa taaaataaag agatgccaaa tcatgtggaa gtagggtctt
     8701 tttttggcat aaaataccgc tccaatggta taagacaagc caccaaataa catgagaaga
     8761 ccaaaagcaa gactagtttt ttgaatgatg actggcaaga taaacacaac gagccagccc
     8821 ataacgatat aaagcatcaa actgaatttt tcattgatac gtttggcaaa aattttgtat
     8881 aaaatcccaa aaagcgtaat gccccactgc aaaacaataa tgatatagcc tagccaacca
     8941 gaaacaaggg ataaggcaac aggtgtataa cttcctgcga tagcaatgta aatcatactg
     9001 tgatcaataa tgcgtagaat gtatttgtga accgaaccat aagccatgga gtggtaaatc
     9061 gtagaggaca aaaacatcaa aaagagactg atgacaaaga tggagatacc aatagcggct
     9121 ttaaggtcat aagtttgata agcatagctg gctgaaatag gaagtagaat gagcatagca
     9181 aatgctccga tagcgtgtgt cacgctgttt gctacttcct cactaaaaga taagggtaaa
     9241 ctttgtttaa aggtttggtt cattgggtca cctcctgtga taaataaata gttttgtggt
     9301 aatctttgac cgtttgacag gcaacctgca caatcggtgg aatgtcctcc tcaatgggag
     9361 ccttctgatt ccaacaggtt acaaaagtat tgtaaagact ttcagaatca gcctgtgcct
     9421 gaagttcctc caatacgcga ctaatatctt gaataggaaa taccgtttta aacaaactga
     9481 tggcaattaa gcgcgctagt tgttgctttt ggtatttttt cttgataggt ttagtcacat
     9541 acccatgctt gacgtaatta ttaatcatag atgccgttag tgatttatta tctgacactt
     9601 ctgaaaaatc agtgcactgg ttgacataaa gtaagacttg atccaaatat aagtctaaat
     9661 cagggaggtc cttccaataa ggtaatgatg gaaaaatcat agttttgtct ctctatctag
     9721 ttttgataac tagattatac gattaatgaa aacttattgc aagcaaaaac tgtaaagact
     9781 taaaagagga aagtagaaat ggcgaagtga agctaatcat gctataatgt taaagaattg
     9841 attgggatag atgccttctg tcgcaatcag actattttat cacgaagaag atgatgctac
     9901 gaaaggaatg atacactcat ggcaatgatt caatggtttc ctgggcacat gtccaaagcc
     9961 agaagacagg ttcaggaaaa tgtaaagcat gttgactttg taactatttt agtggatgca
    10021 cgcttaccac tttctagtca aaatcccatg cttacaaaaa ttgttggtga taaacctaaa
    10081 ttaatgattt taaataaagc tgacttggca gatgcaactc gaactaaaga gtggaaggct
    10141 tattatgaaa gtcaaggtat taaaacatta gccatcaatt ccaaagaaca atcaacggtt
    10201 aaaaaagtaa cagaagcagc caaggaactc atggctgata aaattcaacg cttgcgagag
    10261 cggggcattc aaaaagaaac cttgcgaacc atgattattg gcattccaaa tgctggtaaa
    10321 tctaccttaa tgaatcgctt ggctggtaag aaaattgctg tagttggcaa taaacctggt
    10381 gtgactaaag gacaacaatg gttgaagtcc aataaggaat tggaaattct agacactcct
    10441 ggtattttat ggccaaaatt tgaggacgaa ttggtgggct taaaattagc tttaacaggt
    10501 gctatcaagg accaattact accaatggat gaagtgacta tctttggctt gaattacttt
    10561 agggaatact accctaatcg tttaaccaaa cgttttaaga atattcctct tgaggaagaa
    10621 gctcctgaaa tcattatgac cttgacacgc caacttggat ttaaagacga ttatgaccgt
    10681 ttttacactt tattcgttaa ggaagttcgt gatggtaaat taggtcaata caccttagat
    10741 caggtgggag atatggatgc cgactagtat taaagctatt aaagaaagct tagaggccgt
    10801 tactagcctc ttagaccccc tctttcaaga attggcaacc gacactaggt taggcgtcca
    10861 aaaagctcta aaaagccgac aaaaggctat tcaggccgag ttagcagaag aagaacgatt
    10921 agaagccatg ctttcttatg aaaaagctct ttataaaaaa ggttatcaag ccattgcagg
    10981 tattgatgag gtgggacgtg gtcccttagc aggtcccgtt gtggcagctt gtgtgatttt
    11041 acctaagtat tgtaaaatta aaggccttaa tgattctaaa aaaatcccta aagctaagca
    11101 tgagaccatt tatcaggcag tgaaagaaaa ggctttggct atcggtatcg gtattattga
    11161 caatcagctg attgatgagg tcaatattta tgaagcaacc aaactagcta tgctagaagc
    11221 cattaaacag ttggagggcc aactcacaca accagattat ctcttgattg atgccatgac
    11281 attggatatt gctatttcgc agcagtctat tcttaaaggc gatgccaatt ccttgtctat
    11341 tgcagcagca tcaattgtag ctaaggtcac cagagatcag atgatggcta actatgatcg
    11401 catttttcct ggttatggct tcgctaaaaa tgcaggctat ggcaccaaag aacatttaca
    11461 gggattaaaa gcttacggca taacgcctat ccatcgtaaa agttttgaac ctgttaaatc
    11521 catgtgctgc gattcaacta atccttaaaa agttctgttt ttagcagaat ttttttcttt
    11581 tttacgaata aatgaagtaa ggaggtaact tagtgaatca ttttgaactt tataagctaa
    11641 agaaagctgg actgactaat aaaaacattc tcaatattct tgactaccaa aaacaacagg
    11701 aaaaatcgtt gtcacttcga gatatggccg ttgtttctgg ctgtaagcat ccgtctcact
    11761 ttatagaagc ctataagcag ttagatattc aaaaattaaa aatggaattt aaacaatttc
    11821 ctagtatttc tatcttagat aagcattacc caatggcatt gaaagaaata tacaatccac
    11881 ctgtcctctt gttttttcag ggaaatttag accttcttga gaaacctaaa ttagccattg
    11941 tcggctccag acgctcaagc gataccggag taaagtctgt ccgtaaaatt cttaaagaac
    12001 tcgggaaccg ttttgtgatt attagcggac ttgctcgagg tatcgacact agtgctcatt
    12061 tagcctgcct taaaaatggg ggacaaacca tcgctattat tggaacaggg ttggatcgct
    12121 tttaccctaa agaaaatagg gagttgcaaa ctttcttagg gaaaaatcat cttgtgataa
    12181 cagaatacgg tccaggagaa gaagctttat cttatcactt tccagaacgg aatcgtatta
    12241 tcgcaggcct tagccgaggt attcttgtcg ttgaagcaaa aaatcgttca ggttccttga
    12301 ttacttgcca aattgggata gaagaaggcc gagacatttt tgctgtccca ggaaacattt
    12361 tggacgggaa atccgaaggt tgccttcagt taattaaaga gggagcaaca tgcgtcacgt
    12421 cgggcatgga tatcctttca gagtactaaa aaagttaaag tttcttccta tatagcattt
    12481 tttattgaca gtttcttaaa aatgggatat tatgctataa gctttaaaca aactgttata
    12541 ggaagtgtgt cgaattggga acaaaaacta caactaagcc aaaaacgggt actaaaaagt
    12601 ccaccactaa aaagaaatca acagcgaaaa aaaatctcgt tattgttgaa tcacctgcca
    12661 aagctaagac tattgaaaaa tatcttggac gcagttataa agttgttgct tcggtaggac
    12721 atattcgtga cttaaaaaaa tcgtctatgt ctattgattt tgataataat tatgagccac
    12781 aatacattaa tatccgtggt aagggacccc ttatcaattc tttaaaaaaa gaagccaaag
    12841 ctgctaaaaa agtttattta gctagtgacc cggatcgtga gggagaggct atttcttggc
    12901 atttgtcaca tatcttagga cttgatcctc aagataataa ccgagtggtt tttaatgaaa
    12961 ttacgaaaga tgcagttaaa catgcctttg tggagcctcg tcagattgat atggacttgg
    13021 tagattctca acaagcacgt cgtgttttgg accgtattgt gggatattcc atttcaccaa
    13081 ttctttggaa aaaggtcaaa aaaggacttt cag
//
LOCUS       DAUCLR010000052        12610 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.052, whole genome shotgun sequence.
ACCESSION   DAUCLR010000052 DAUCLR010000000
VERSION     DAUCLR010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12610
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.052"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            8..1171
                     /locus_tag="VGT87_001226"
     CDS             8..1171
                     /locus_tag="VGT87_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEQ3814080.1"
                     /translation="MATVVALIVALLLFGLIYDLLGVQKNELAAQKSAQPKVKTARVV
                     ANGDILIHDILYMSARKADDTYDFTPYFEYVKDWISGADLAIGDYEGTISPDYPLAGY
                     PLFNAPEEIAGALKNTGYDVVDLAHNHILDSQLDGALNTKKVFHQLGIDSIGIYDKDR
                     SKESFLIKNVNGIKIAILGYSYGYNGMEATLSQEDYEKHMSDLDEAKIKKELQLAEKK
                     ADVTIVMPQMGTEYALEPTAEQKELYHKMIDWGADVVLGGHPHVIEPSETVIKGRQKK
                     FIIYSMGNFISNQRLETVDDIWTERGLLMDLTFEKKDNKTKIKTVEAHPTMVLAKGKG
                     IVGKEGFELYNYRTMVLEDFIKGGKYHDKIDEETKAKVALAYQEINDLVNLKW"
     misc_feature    1276..1350
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            1385..1699
                     /gene="rplU"
                     /locus_tag="VGT87_001227"
     CDS             1385..1699
                     /gene="rplU"
                     /locus_tag="VGT87_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEQ3814081.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAIYVEKIDAEAGAEVTFNEVVLVG
                     GDKTVVGTPVVEGATVVGTVEKQGKQKKVVTFKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            1711..2037
                     /locus_tag="VGT87_001228"
     CDS             1711..2037
                     /locus_tag="VGT87_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEQ3814082.1"
                     /translation="MIKAIFTRQKNGQLSSVTLTGHAGSGKHGFDIVCASVSTLAINF
                     VNSLEVLADCQALVDLNDVEGGYMAITIPPHDNKEEVQLLFESFLLGMTSLAKDSSKF
                     VNTQVI"
     gene            2065..2358
                     /gene="rpmA"
                     /locus_tag="VGT87_001229"
     CDS             2065..2358
                     /gene="rpmA"
                     /locus_tag="VGT87_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEQ3814083.1"
                     /translation="MLKMNLANLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVSG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPVAK"
     gene            2706..3620
                     /locus_tag="VGT87_001230"
     CDS             2706..3620
                     /locus_tag="VGT87_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEQ3814084.1"
                     /translation="MNIQQLRYVVAIANNGTFREAASKLFVSQPSLSVSIKDLEAELG
                     FQIFNRTTSGTVLTSQGLVFYEKALEVVKSFDSFEKTFSQADLDQNEFSIASQHYDFL
                     PPLITAFSQQYDGHRVFRIFESTTIQILDEVAQGNSEIGIIYLNVDNQKGLFQRMDKL
                     GLEYVSLIPFTTHIYLSKTHPLANREALYLNDIQGLPAVRFTQERDEYLYYSENFVDT
                     SECPRIYNVSDRATLNGILERTNAFATGSGFLDHRSVNGIKVIPLADHIDNQMIYVKR
                     KDKNLSVAGATFVTILKDYFDKRRKALT"
     gene            3617..4075
                     /locus_tag="VGT87_001231"
     CDS             3617..4075
                     /locus_tag="VGT87_001231"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEQ3814085.1"
                     /translation="MKKRLFVPSLILLVALDQLSKFWIVSHIALGEVKPFIPGIVSLT
                     YLQNNGAAFSILQDQQWFFVVITVLVIGYAIYYLATHPHLNIWKQLALLLIISGGIGN
                     FIDRLRLAYVIDMIHLDFMDFAIFNVADSYLTVGVILLVICLWKEEDYGN"
     gene            4065..4955
                     /locus_tag="VGT87_001232"
     CDS             4065..4955
                     /locus_tag="VGT87_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEQ3814086.1"
                     /translation="MEINVITSGQRLDKALADLSPLSRGQANDQIKQGLVLVNGQQKK
                     AKYTVQAGDVICFELPKEEVLEYQAQNIPLDIIYEDDALAIINKPQGMVVHPSAGHPS
                     GTMVNALMYHIKDLSSINGVVRPGIVHRIDKDTSGLLMVAKTDAAHQALAEELKAKKS
                     LRKYLAIVHGNLPNDRGMIEAPIGRSEKDRKKQAVTAKGKEAVTRFTVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDPLYGPRKTLSGHGQFLHAKTLGLTHPMTGKEM
                     IFTVEAPEIFQKVLKLLRKK"
     gene            5351..5872
                     /gene="pyrR"
                     /locus_tag="VGT87_001233"
     CDS             5351..5872
                     /gene="pyrR"
                     /locus_tag="VGT87_001233"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEQ3814087.1"
                     /translation="MKTKEIVDDVTMKRAITRITYEIIERNKQLDNVVLAGIKTRGVF
                     LARRIQERLHQLEGLDLPIGELDTKPFRDDMRVEEDTTLMSVDITGKDVILIDDVLYT
                     GRTIRAAIDNLVSLGRPARVSLAVLVDRGHRELPIRADYVGKNIPTSSVEEIVVEVVE
                     VDGRDRVSIIDPT"
     gene            5888..7147
                     /locus_tag="VGT87_001234"
     CDS             5888..7147
                     /locus_tag="VGT87_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEQ3814088.1"
                     /translation="MKDVSYDVEEVPKAGMLVGLSFQHLFAMFGATVLVPILVGIDPS
                     VALLSSGLGTLAHLSVTKFKIPAYMGSSFAYIAAMQLLMKTNGIGAVAQGAMTGGLVY
                     LIVALIVKAIGNDWIDNILPPIVVGPIVMVIGLSLASTAVNDVMLKNGNYNLTYLVIG
                     LVTLLSVIFFNIYGKGIVAIVPILLGLLVGYVVALLVGVFTGQEIVDFTNVAQAKWFS
                     IPSVEIPFLTYGVKFYPSAILTMAPIAFVTMTEHFGHIMVLNSLTKRDYFKDPGLEKT
                     LTGDGFAQIIAGFLGAPPVTSYGENIGVMALNKILSVYVIAGAAVIAALLSFIGKVSA
                     LIQSIPTPVIGGISVALFGVIASSGLKILIESKVDMDNKKNLLIASVILVSGIGGLML
                     QVNGLQISGVAFSTLLGIILYQVLPEK"
     gene            7208..8143
                     /locus_tag="VGT87_001235"
     CDS             7208..8143
                     /locus_tag="VGT87_001235"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEQ3814089.1"
                     /translation="MSVVNNRVALTNLVSMEALTTEEVLGLINRGSEYKAGKVVISDH
                     QKDLVANLFFENSTRTHKSFEVAEKKLGLTVLDFNADASAVNKGESLYDTVLTMSALG
                     TDICVIRHPEDDYYKELVESPTITASIVNGGDGSGQHPSQCLLDLLTIYEEFGRFEGL
                     KIAIAGDLTHSRVAKSNMQILKRLGAELYFYGPEEWYSEAFNAYGTYIAIDQIIKELD
                     VLMLLRVQHERHDGHQSFSKEGYHQAFGLTQERYQQLKDSAIIMHPAPVNRDVEIADS
                     LVEAPKARIVSQMANGVFVRMAIIEAILNGRNKNS"
     gene            8187..9269
                     /gene="carA"
                     /locus_tag="VGT87_001236"
     CDS             8187..9269
                     /gene="carA"
                     /locus_tag="VGT87_001236"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEQ3814090.1"
                     /translation="MTKRLLILEDGTIFEGEPFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLIGNYGINRDDYESISPTCKGVVVSEVSRLASNWRKQMTLDAFLK
                     IKGIPGISGIDTRALTKIIRQHGTMKATMADDGDSIQHLKDQLRATVLPTNTIEQVST
                     KTAYPAPGIGKNIVLVDFGLKHSILREFSKRQCNITVVPFNITAEEVLQLNPDGLMLS
                     NGPGNPEDLPEALDMIRGVQGKIPIFGICMGHQLFSLANGAKTCKMTFGHRGFNHAVR
                     EIATGRIDFTSQNHGYAVERSSLPDTLMVTHEDINDKTVEGVKHRDFPAFSVQFHPDA
                     APGPHDASYLFDEFLEMIDSWRCTSK"
     gene            9515..>12610
                     /gene="carB"
                     /locus_tag="VGT87_001237"
     CDS             9515..>12610
                     /gene="carB"
                     /locus_tag="VGT87_001237"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004218948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEQ3814091.1"
                     /translation="MPKRKDIQKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYKV
                     ILVNSNPATIMTDKEIADKVYIEPLTLEFVNRIIRKERPDAILPTLGGQTGLNMAMAL
                     SKAGILDDLEIELLGTKLSAIDQAEDRDLFKQLMQELDQPIPESTIVKTVDEAVTFAR
                     DIGYPVIVRPAFTLGGTGGGICSSEEELCEITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPTQTLSDIENQMLRDASLKIIRALK
                     IEGGCNVQLALDPYSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     INPITGTTYAMFEPTLDYVVAKIPRFPFDKFEHGERQLGTQMKATGEVMAIGRNLEES
                     LLKACRSLEIGVCHNEMTSLSNISDEELVTKVIKAQDDRLFYLSEAIRRGYSIEELES
                     LTKIDLFFLDKLLHIVEIEQDLQMHVEHLESLKKAKRYGFSDQKIAEIWQKDESDIRA
                     MRHSHSLYPVYKMVDTCAAEFDAKTPYFYSTYELENESVQSNKESILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQKAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAQALSEAGVTILGTQVEDLDRAEDRDLFEKALKELGIPQP
                     QGQTATNEEEALEAAKKIGFPVLVRPSYVLGGRAMEIVENKEDLREYIRTAVKASPEH
                     PILVDSYIFGKECEVDAISDGKSVLIPGIMEHIERAGVHSGDSMAVYPPQQLSKQIQE
                     TIAEYTKRLAIGLNCIGMMNVQFVIKNEQVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKLILGQTLKDLGYEDGLYPQSQLVHIKAPVFSFTKLAQVDSLLGPEMKSTGEVMGSD
                     TSLEKALYKAFEANNSHLSEFGQIVFTIADDSKAEALSLARRFKAIGYQIMATQGTAA
                     YFAEQGLSACLVGKIGDAANDIPTLVRHGHVQAIVNTVGIKRTADKDGQMIRSSAIEQ
                     GV"
BASE COUNT         3924 a         2127 c         2670 g         3889 t
ORIGIN      
        1 aaaaacgatg gcaacagttg ttgctcttat cgtcgcactt ttgctatttg gcttgattta
       61 tgatttactt ggggttcaaa aaaatgagct agcagctcaa aaatctgctc agccaaaggt
      121 caaaacagcg cgtgttgtag ctaatggcga cattttaatt catgatattc tctacatgag
      181 tgctagaaaa gctgacgata cctatgattt cactccttat tttgaatatg ttaaggactg
      241 gattagcggt gctgacttag ctattggaga ttatgaaggg accattagcc cagattatcc
      301 cttagcaggc tatcccttat tcaatgctcc agaagagatt gcaggtgctc tgaaaaatac
      361 tggctatgat gtagtcgatt tagcccataa tcatatctta gattcccaat tagacggtgc
      421 ccttaacact aaaaaggttt ttcatcaatt aggtatagac agtattggca tctatgacaa
      481 ggatcgttca aaagaatcct tcttaattaa aaatgtcaat ggtatcaaaa ttgcaatttt
      541 aggttattct tatggttata acggcatgga ggccacactt agccaagaag actatgagaa
      601 acacatgtct gatttagatg aagctaagat aaaaaaagaa cttcagctag ctgaaaaaaa
      661 ggctgacgtg accattgtca tgccacaaat gggaacagaa tatgccttag aaccgacagc
      721 agagcaaaaa gaactttacc acaaaatgat tgattgggga gctgacgtcg tcctaggagg
      781 ccatccgcat gttattgaac cttcagagac agttataaaa ggtaggcaaa agaaattcat
      841 tatttactct atgggaaatt ttatttcaaa tcagcggctt gaaaccgtag atgatatctg
      901 gactgagcgt ggcctattga tggaccttac ttttgagaaa aaagataaca aaacaaaaat
      961 taaaacagtt gaggcccatc caaccatggt tttagccaaa ggtaaaggca ttgtgggtaa
     1021 agaaggtttt gaactataca attatcgaac aatggtttta gaagatttta tcaaaggtgg
     1081 aaaataccac gacaagattg atgaggaaac caaagcaaaa gtagcacttg cttatcagga
     1141 aattaatgat ttagttaacc tgaaatggta atgtttggtg gtttagcatc taacgataaa
     1201 aaatagagat taaacttaat ttttccttga taaaaagcag ttatggtgct aaaatagtaa
     1261 cgttgactaa atgcacatcc tgtgcaaccg cgcgaaaatc gttgtcaagt agaccaagtc
     1321 tcacgattcc aggcgagtct tcacagaggg aaacctcaaa caaaaatttt ataggaggtg
     1381 catcatgagc acatacgcaa tcatcaaaac tggtggaaaa caagttaaag ttgaagtagg
     1441 tcaagcaatc tacgttgaga aaattgacgc tgaagctggc gcagaagtca cttttaacga
     1501 agttgttctt gtaggtggtg acaaaactgt agttggtact ccagttgttg aaggagctac
     1561 tgtcgttgga actgttgaaa aacaaggaaa acaaaagaaa gttgtaacat tcaaatacaa
     1621 acctaaaaaa ggtagccacc gtaaacaagg tcatcgtcaa ccttacacta aagttgtcat
     1681 caacgcaatc aacgcttaat ttactagctt atgattaaag caatttttac tcgccaaaaa
     1741 aatggtcaac tgagcagtgt tacactgaca ggtcatgctg gtagtggtaa acatggcttt
     1801 gatattgttt gtgcctctgt tagtactcta gccattaact ttgttaattc tcttgaggtg
     1861 ttagcagatt gccaagcatt agtagatctt aatgatgttg aaggtgggta tatggctatt
     1921 acaataccac cacatgacaa caaggaggaa gttcaattat tatttgaatc ttttcttcta
     1981 ggaatgacca gtttggcgaa ggactcttcg aaatttgtca acactcaggt catctaaatt
     2041 taatttaaga gaggaaacaa cattatgtta aaaatgaatc ttgctaactt gcaacttttc
     2101 gcccacaaaa aaggtggagg ttctacatca aacggacgtg actcacaagc taaacgcctt
     2161 ggtgctaaag ctgctgacgg tcaaactgtt tcaggtggat caatccttta ccgtcaacgt
     2221 ggaactcata tctacccagg tgtaaacgta ggccgtggtg gagatgacac actttttgct
     2281 aaagttgaag gtgttgtacg tttcgaacgt aaaggacgcg ataaaaaaca agtttctgtt
     2341 tacccagtag ctaaataatt gtttaatatc gcattatata aggctttcca ggtgtctgga
     2401 aagccgattt ttttattttg ctactcattt gctacccata ttctaaaagc cgatgtagga
     2461 agagagcttg tcagcagttt tatcttttga ggatttagta acatgagcgt agatattcat
     2521 ggtagtttgg atagtttcac aaccaagtgt attggatttt cttctatcct actaaagatt
     2581 gtagttgctt atttagagca atagttctaa ttgtaaaaag tcagcataat aaaacaatat
     2641 cataagatgt tgttataacg ggctttatgg tataattaag gatactcatt ttaggaagga
     2701 aagcaatgaa tattcaacaa ttacgctatg tggtagccat cgctaataat ggaacttttc
     2761 gagaagctgc ctctaaattg tttgtcagtc agcctagctt atcagtttct atcaaagatt
     2821 tagaggcgga attagggttt caaattttta atcggactac ttcaggaaca gtcttgacaa
     2881 gtcaaggact agttttttat gagaaagctt tagaagtagt caaatctttt gattcttttg
     2941 aaaaaacctt ttctcaagct gatctggatc aaaatgaatt ttctattgct agccaacatt
     3001 atgacttttt gccacctctt attacagctt tttcgcaaca atacgatggt catcgtgtct
     3061 ttcgtatttt tgaatcaaca actattcaaa ttttagatga agttgctcaa ggaaacagtg
     3121 aaattggtat tatttacttg aatgtagaca atcaaaaagg tctctttcag cggatggata
     3181 agctgggctt ggaatatgtt tctctaattc ctttcactac tcatatttat ctttcaaaaa
     3241 cgcatccatt ggctaatcgc gaagcgcttt atttgaacga tattcaagga ttacctgcgg
     3301 taaggtttac ccaagaaagg gacgagtatt tgtattattc tgaaaatttt gtggatacta
     3361 gtgaatgccc tcgtatttat aatgtgtcag atagggcaac cttgaacggt attttagaga
     3421 gaaccaatgc ctttgcgaca ggatcaggtt ttctcgatca tcggagtgtt aatgggatta
     3481 aagtgatacc tctagctgat catattgaca atcaaatgat ttatgtcaaa cgaaaagata
     3541 aaaacctttc tgttgctggg gccacttttg tgactatttt aaaagactat tttgataaaa
     3601 gaaggaaggc cttaacatga aaaaacgatt gtttgtgcct agcttgatcc tccttgtagc
     3661 tttggatcaa cttagtaaat tttggattgt ttctcatata gcgcttggag aagtgaaacc
     3721 ctttatccca ggtatcgtca gcttgactta cttgcaaaac aatggggctg ccttttccat
     3781 attgcaggac cagcaatggt tctttgttgt cataacggtt ttagttatcg gttatgctat
     3841 ttattacctt gctactcatc cccatttaaa tatctggaag caattagctc tcttgcttat
     3901 tatttctggt ggaatcggga attttattga tcgtttgcgt ttagcttacg tgattgatat
     3961 gattcattta gactttatgg attttgccat ttttaatgtg gcagattcat accttaccgt
     4021 tggtgtcata ttattagtga tatgtttatg gaaagaagag gattatggaa attaatgtga
     4081 taacatcggg gcaacgcttg gataaggcac tagctgattt aagtcccctg tcacgtggtc
     4141 aagccaatga ccaaattaag caaggccttg ttttagttaa tggtcagcaa aaaaaggcta
     4201 aatatacggt tcaagcagga gatgtgattt gttttgaact ccctaaagaa gaagtcttag
     4261 agtaccaagc tcagaatatt cccttggaca ttatctatga agatgatgct ctcgccatta
     4321 ttaacaagcc acagggaatg gtagttcacc cttcggcggg acacccttca ggaacaatgg
     4381 ttaatgcctt gatgtatcat atcaaggatt tgtcctcaat caatggtgtg gttcgtccag
     4441 gaattgttca tcggattgat aaggacacct caggtctctt aatggtagct aaaacagatg
     4501 cagctcatca agccttggca gaagaattaa aggctaaaaa atctctcaga aaatacctcg
     4561 ccattgtcca tggaaaccta ccgaatgatc gcgggatgat tgaagctcct ataggtcgta
     4621 gcgaaaagga ccgtaaaaaa caggctgtta cagcaaaagg caaagaagca gtgacacgtt
     4681 ttacagtgct agaacgtttt ggcgattaca gtctagtgga attacaactg gaaactggtc
     4741 gaacgcacca aattcgtgtt catatggcct atattggtca tcctgttgct ggggaccctt
     4801 tatatggacc acgtaagaca ctatcaggtc atggtcaatt tcttcatgca aaaacattgg
     4861 gattaacgca tcctatgaca ggaaaagaaa tgatatttac tgttgaagct cccgaaattt
     4921 ttcaaaaggt gttaaagctg ctaagaaaaa aataaacagc gaggagtagc cattattttt
     4981 agtcttatat ctttgtgtaa agtttttaac attataaaaa atactaaaaa gcatttacga
     5041 taagccagga aatatgagaa gtcttttatg ttattgggat atgcaacata tctgttagag
     5101 atctttattt aaactcctta aagaaaagag atattccaaa tagatgctag gtattgtctt
     5161 ttttacttgt ttttcttttc tttttttgct atactgattg aaataaaata ttcctttaaa
     5221 acagtcctgt gaggctgtca aggagacgga ttaaaaacat gcactatgat acttaattgt
     5281 gctatatttt gctgaatctc cttgtttcca aggagatctt tttatttgaa tttttagagg
     5341 aggtttcaac atgaaaacta aggaaattgt tgatgacgtg accatgaaac gcgccattac
     5401 ccgtattact tatgaaatta tcgaacgaaa caaacaattg gacaatgtgg ttttggcagg
     5461 catcaaaacg cgaggtgttt ttctagctcg tcgcattcaa gaaagactgc atcaattaga
     5521 agggttggat ttaccaattg gtgagttaga caccaagcct tttagagatg acatgagagt
     5581 tgaggaagac acaactctca tgtcagtaga tattacaggc aaggatgtga ttttgattga
     5641 tgatgtgctt tatactggtc gtaccatccg cgcagccatc gataacttag tgagtttagg
     5701 tcgaccagcc cgtgttagtc tagctgtttt ggtggatcgt ggccatcggg aattaccgat
     5761 tcgcgcggat tacgttggga aaaatattcc aacaagcagt gtagaagaaa ttgttgttga
     5821 ggttgtcgaa gtcgatggcc gtgatcgtgt tagcattatt gacccaacat aagaaagagg
     5881 ttttactgtg aaagatgtca gttatgatgt ggaagaagtg ccaaaggcag gtatgttagt
     5941 tggtttgtcc tttcagcact tatttgctat gtttggtgcg actgttctgg ttcccattct
     6001 agttggaatt gacccatcag ttgctttgtt atctagtggt ttaggaaccc ttgcccactt
     6061 atcagtgacc aaatttaaaa ttccagccta catgggctca agttttgctt atatagcagc
     6121 tatgcagtta ctgatgaaga caaatggtat tggtgctgta gctcaagggg ctatgacagg
     6181 aggtctcgtt tacctgattg tagctttgat tgttaaagct attggcaatg attggattga
     6241 taacatctta ccaccgattg tggttggtcc gattgttatg gtcattggtc taagcctagc
     6301 ttctacagct gttaatgatg tcatgctaaa aaatgggaac tataatctga cttatcttgt
     6361 tattgggtta gtcaccttat tatcagttat ttttttcaat atttatggta aaggaattgt
     6421 tgctattgta ccaattttat tggggctatt agttggttat gttgtagccc ttttagtggg
     6481 tgttttcacc ggtcaagaaa ttgttgattt taccaatgtg gctcaggcca aatggtttag
     6541 tatcccatca gtggaaattc ctttcttaac ctacggggtt aaattctacc caagtgccat
     6601 tttaactatg gcacccattg cctttgttac aatgacagaa cattttggac acattatggt
     6661 cttaaatagt ttgacaaaaa gagattattt caaggatcca ggacttgaaa agactttaac
     6721 tggtgatggt tttgcgcaaa tcattgccgg cttcttaggg gcacctccgg tcacttctta
     6781 tggtgaaaac atcggtgtaa tggctttgaa taaaatatta tcggtctatg ttattgcagg
     6841 tgccgcagtg attgccgctc tcctcagttt tatcggtaag gtatcagcct taattcaatc
     6901 tattccaaca ccagttattg gaggtatatc agttgccttg tttggtgtaa tagcttctag
     6961 cggtttgaaa atcttgattg aatccaaagt tgatatggat aataagaaaa acttattgat
     7021 tgccagtgtt attttggtct ctgggattgg aggattgatg cttcaagtaa atggtcttca
     7081 aatttcaggt gtcgctttct caacacttct tggcatcatc ttatatcagg tacttcctga
     7141 aaaataggaa gaattgttgt tagaccactt acatagtcat cagtcgttaa tgataaaggg
     7201 gataatcatg tcagttgtaa ataatcgcgt tgcacttacc aatttagttt caatggaagc
     7261 attaaccacc gaagaagttt taggtcttat caaccgtggt tcagaatata aggctggtaa
     7321 ggttgttatt tcggatcatc aaaaggatct tgttgccaat ttattctttg aaaactccac
     7381 ccgaacccat aagtcttttg aagtggctga gaagaaactc ggcttgacag ttcttgattt
     7441 caatgctgat gcaagtgcgg ttaacaaggg agaaagtttg tatgatactg tgttaaccat
     7501 gagtgcactt ggaacagaca tctgtgtgat tcgtcaccca gaagatgatt attacaagga
     7561 attagtagaa agtccaacta ttacagcttc cattgtcaat ggaggagatg gttctggaca
     7621 acacccgagt caatgtttac ttgacttatt aaccatctat gaagaatttg gacgctttga
     7681 aggcttaaag attgcgatcg ctggtgactt aactcactct agggtggcaa agtccaacat
     7741 gcaaatcctc aaaagattag gagcagagct ttatttctat ggacctgaag aatggtattc
     7801 agaagccttt aatgcttacg gcacttatat tgctattgac cagattatta aggaattgga
     7861 tgttcttatg ttgctgcgtg tacagcacga acgtcatgac ggccatcaga gtttttcaaa
     7921 agaaggttac catcaagcgt ttggattgac acaggaacgt taccaacaat taaaagattc
     7981 tgctattatt atgcatccag ctccagttaa tcgggatgtg gaaattgcag atagtttggt
     8041 ggaagctcct aaagcgagaa ttgtatctca gatggctaat ggtgtctttg ttcgcatggc
     8101 tattattgaa gccattttga atggtagaaa taaaaactca tgatgaaaga ttaaagcatg
     8161 tttagaaggt aggaaaaggt aggaaaatga caaaacgatt acttatttta gaagacggca
     8221 ctatttttga aggagaaccc tttggtgctg atattgatgt gactggtgaa attgtcttta
     8281 atacaggaat gacaggctac caagaatcca tcactgacca atcctacaat gggcaaattt
     8341 taacctttac ctatcctttg attggaaatt atgggattaa tcgagatgac tatgagtcca
     8401 tttcacccac ctgtaaaggg gttgttgtgt ctgaagtcag tcggttggca agtaattggc
     8461 gcaagcaaat gacgctagat gcgttcttga aaataaaagg gattccaggg atttcgggaa
     8521 ttgatacgag agctttgacc aaaattattc ggcaacacgg taccatgaaa gctacgatgg
     8581 cagatgatgg agatagtatc cagcatttaa aagatcaact gcgtgcaaca gtcttaccga
     8641 ctaacaccat tgaacaagtg tcaactaaaa cagcttatcc tgcaccaggg atcgggaaaa
     8701 atattgtatt ggttgatttt ggtttaaaac attctatttt aagagaattt tctaaaagac
     8761 aatgtaatat caccgttgtc cctttcaaca tcactgcaga agaagtgctc caactaaatc
     8821 cggatggttt gatgctatct aatggcccag gaaacccaga agatttgcca gaggctttag
     8881 acatgattcg tggggtacaa ggtaagattc ctatttttgg tatttgtatg ggacatcaac
     8941 tctttagtct agcaaatggt gccaagactt gtaaaatgac ctttggtcat cgtggcttta
     9001 accatgccgt tagagaaatt gcaacgggaa gaattgattt taccagtcaa aaccatggtt
     9061 atgccgttga acgtagtagt ctacctgata ctttaatggt tacgcatgaa gacattaacg
     9121 ataagacggt cgaaggagtg aaacatagag attttccagc tttttcagtc caatttcacc
     9181 ctgatgcggc tccaggacct catgatgcca gctacttatt tgacgaattt ttggaaatga
     9241 ttgattcttg gagatgtacg agtaaatagt aaatcagttg caatttcacg actatatcac
     9301 ctcttgttac tctatttatt aacatttaca gttgatttag taccctatca aaaatattat
     9361 ttaatgttgc tctgtgaggc aacaaataga gaaaggaagg caaggagaac acaagtaaat
     9421 aatttagtaa aaagttaggt tttcaaagag ataaagctct tattgagaag tgctgacttt
     9481 accgcttctt gtggtattct tggtatcatt aattatgcca aaacgaaaag atattcaaaa
     9541 aatcatggtt atcgggtctg gcccgattat tattggacaa gctgctgaat ttgattatgc
     9601 agggactcag gcctgtttgg ctctaaaaga agaaggctat aaggtcatac ttgttaattc
     9661 aaatcctgca acgattatga cagataaaga aatcgctgat aaggtatata ttgagccttt
     9721 gactcttgaa tttgttaatc gtatcattcg aaaagaaagg ccagatgcca tactgccaac
     9781 cttaggtggt caaacgggcc tcaatatggc aatggcatta tctaaagctg gtattcttga
     9841 tgatttagag attgaattgt taggtactaa gttatctgcg attgatcagg cagaagacag
     9901 agaccttttc aaacaattaa tgcaagaact agaccaaccc attccagaat ctacaattgt
     9961 taaaacagtt gatgaagctg ttacatttgc tagagatatc ggttatccag tcattgtgcg
    10021 cccagctttt actcttggtg gaactggtgg aggaatatgt agtagtgaag aagaactttg
    10081 tgaaattact gaaaacggcc taaagttatc accggtcaca cagtgtttga ttgaacgctc
    10141 aattgctggc ttcaaagaaa ttgaatacga agtaatgcga gatagtgctg ataacgcgct
    10201 ggttgtttgt aatatggaaa actttgatcc cgtagggatt cacacaggag attctattgt
    10261 atttgcacca acacagaccc tttctgatat tgaaaatcaa atgttacgtg atgctagttt
    10321 aaaaataatt cgagctctga aaattgaagg tggctgtaac gtccagttag cactagatcc
    10381 ttatagtttc aaatattatg ttattgaagt aaatcctaga gtgtctcgtt cctctgccct
    10441 tgcttcaaaa gcaacaggtt atcccattgc caaattggct gcaaagattg ctgttggcct
    10501 aacacttgat gaaatgatta accctatcac aggcactact tacgctatgt ttgaacctac
    10561 ccttgattac gttgttgcta aaattccgcg ttttccgttt gataaatttg aacatggtga
    10621 acgccaactg ggaactcaaa tgaaagcaac aggtgaagtc atggctattg gccgtaattt
    10681 ggaagagtcc ttactaaaag cctgtcgttc tttagaaatt ggtgtttgcc ataatgagat
    10741 gacaagttta agtaacatta gtgatgaaga gttagtgact aaagtgatta aagctcaaga
    10801 tgatcgactt ttttacttgt cagaagcaat acgacgaggt tatagtatcg aagaacttga
    10861 gagcttgacc aagattgatt tatttttctt ggataaatta cttcatattg ttgaaataga
    10921 gcaagatcta caaatgcatg ttgagcattt ggaaagcctc aaaaaagcaa agcgttatgg
    10981 attttcagat caaaaaattg cagaaatttg gcaaaaggat gaaagtgata tcagagcgat
    11041 gagacattct catagcctgt atcctgtcta taaaatggta gatacttgtg cagctgaatt
    11101 tgatgctaaa accccttatt tttattcaac atatgagttg gaaaatgaat ctgttcagtc
    11161 gaataaagaa tcaattttag ttttaggttc aggccctatt cgtattggtc aaggggttga
    11221 atttgactac gcaacggttc attctgttaa agccatccaa aaagctggct atgaagccat
    11281 tatcatgaat tcaaatccgg agactgtatc tacagatttt tcggtatcag ataaattata
    11341 ttttgaaccg ttaacctttg aagacgtcat gaatgtgata gatttagagc agcctaaggg
    11401 agtaatagtt cagtttggag gacaaactgc tattaactta gcacaagcac tttctgaggc
    11461 gggtgttaca atattaggaa cacaagtaga ggatttggat agggctgaag acagagattt
    11521 atttgaaaag gcgttgaaag agcttggaat cccacaacca caaggtcaaa cggcaactaa
    11581 tgaagaagaa gctttagagg cagctaaaaa aattggtttt ccagtcttag ttagaccttc
    11641 ttacgtactt ggtggccggg ctatggaaat tgttgaaaac aaagaagatc ttagagaata
    11701 tataagaaca gcggttaaag caagtcctga acatcctatc ttagtggact cttatatttt
    11761 cggtaaggag tgtgaagtgg atgccatttc agatggaaaa tcagtcttaa ttcctggtat
    11821 tatggagcac atcgaaagag caggtgttca ttcaggagat tcaatggcag tttatccgcc
    11881 acagcaatta tctaagcaaa ttcaagaaac aatagctgag tatactaaac gtttagccat
    11941 tgggttaaat tgtattggta tgatgaatgt tcaatttgtt attaaaaatg aacaagtcta
    12001 tgttattgaa gtgaatccac gcgctagtcg taccgtacct ttcttatcaa aagtaacagg
    12061 tattccgatg gctcagattg ctactaagtt gattttaggt caaacattaa aggatctagg
    12121 atatgaagat ggtctttatc cccaaagtca actggtgcat atcaaagcgc ctgtgttttc
    12181 atttacaaag ttggcacagg ttgatagtct tttaggtcct gaaatgaaat caactggaga
    12241 agtaatgggt tcggatacta gtcttgaaaa agcactttat aaagcgtttg aagccaataa
    12301 ttctcattta tcagaatttg gtcaaattgt ctttactata gcagatgata gtaaggcaga
    12361 agctctaagt ctagcaagac gatttaaggc gattggctat caaattatgg ctactcaagg
    12421 tacagcagct tactttgctg agcaaggctt aagcgcgtgt ttagttggaa aaattggtga
    12481 tgctgccaat gacatcccga cactcgtgcg acatggtcac gttcaggcaa ttgttaatac
    12541 agttggaatt aaacgcacag ctgataaaga cggacagatg attagaagtt cagctattga
    12601 acaaggagtt
//
LOCUS       DAUCLR010000053        12568 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.053, whole genome shotgun sequence.
ACCESSION   DAUCLR010000053 DAUCLR010000000
VERSION     DAUCLR010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12568
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.053"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            99..803
                     /locus_tag="VGT87_001238"
     CDS             99..803
                     /locus_tag="VGT87_001238"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEQ3814092.1"
                     /translation="MTNQLIYKGKAKDIYSTKDENVIRTVYKDQATMLNGARKETIDG
                     KGALNNQISSLIFEKLNKAGVVTHYIEQISKNEQLNKKVDIIPLEVVLRNVIAGSFSK
                     RFGVEEGRVLETPIVEFYYKNDDLNDPFINDEHVKFLGIVNDEEIAYLKGETRRINEL
                     LKGWFAQIGLNLIDFKLEFGFDQEGTIILADEFSPDNCRLWDKNGNHMDKDVFRRDLG
                     NLTDVYQVVLEKLIAL"
     gene            927..4652
                     /locus_tag="VGT87_001239"
     CDS             927..4652
                     /locus_tag="VGT87_001239"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEQ3814093.1"
                     /translation="MNKRIFVEKKADFGIKSASLVKELTHNLQLTSLKALRIVQVYDV
                     FNLAEDLLARAEKHIFSEQVTDCLLTETEITAELDKVAFFAIEALPGQFDQRAASSQE
                     ALLLFGSDSQVKVNTAQLYLVNKDITEAELEAVKNYLLNPVDSRFKDITLPLEEQAFS
                     VSDKTIPNLDFFETYQADDFATYKAEQGLAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKNIDFSASKFQKQLQTTYDKYIAMRDELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDDVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITTPIAETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKENVVREKPEAGDVVILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRDGEVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLDKVPLKYQGLNGTEIAISESQERMSVVVRPSDVDAFIA
                     ACNKENIDAVVVATVTEKPNLVMTWNGEIIVDLERRFLDTNGVRVVVDVKVVDKDLTV
                     PEARTTSAETLEADTLKVLSDLNHASQKGLQTIFDSSVGRSTVNHPIGGRYQITPTES
                     SVQKLPVQHGVTTTASVMAQGYNPYIAEWSPYHGAAYAVIEATARLVATGADWSRARF
                     SYQEYFERMDKQAERFGQPVSALLGSIEAQIQLGLPSIGGKDSMSGTFEDLTVPPTLV
                     AFGVTTADSRKVLSPEFKAAGENIYYIPGQAISEDIDFDLIKDNFSQFEAIQAQHKIT
                     AASAAKYGGVLESLALMTFGNRIGASVEIAELDSSLTAQLGGFVFTSAEEIADAVKIG
                     QTQADFTVTVNGNDLAGAGLLAAFEGKLEEVYPTEFEQADALEEVPAVVSDAVIKAKE
                     TIEKPVVYIPVFPGTNSEYDSAKAFEQVGSSVNLVPFVTLNEAAITESVDTMVANIAK
                     ANIIFFAGGFSAADEPDGSAKFIVNILLNEKVRAAIDSFIENGGLIIGICNGFQALVK
                     SGLLPYGNFEEAGETSPTLFYNDANQHVAKMVETRIANTNSPWLAGVEVGDIHAIPVS
                     HGEGKFVVSASEFAELRDNGQIWSQYVDFDGQPSMDSKYNPNGSVNAIEGITSKNGQI
                     IGKMGHSERWEDGLFQNIPGNKDQTLFASAVKYFTGK"
     gene            4813..6267
                     /locus_tag="VGT87_001240"
     CDS             4813..6267
                     /locus_tag="VGT87_001240"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEQ3814094.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPQAAQVTYFGLHSLQHRGQEGAGI
                     VSNDNGKLYGYRNVGLLSEVFKNQSELDNLTGNAAIGHVRYATAGSADIRNIQPFLYK
                     FHDGQFALCHNGNLTNAISLRKELEKQGAIFNASSDTEILMHLIRRSHNPNFIGKVKE
                     ALNTVKGGFAYLLMTENKLIAALDPNAFRPLSIGQMQNGAWVISSETCAFEVVGAKWV
                     RDVEPGEVILIDDSGIQCNRYTDETQLAICSMEYIYFARPDSTIHGVNVHTARKNMGK
                     HLAQEFKQDADIVIGVPNSSLSAAMGFAEESGLPNEMGLVKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMIDDSIVRGTTSRRIVGLLREAGASEVHVAIASPELKY
                     PCFYGIDIQTRRELISANHAADEVCDIIGADSLTYLSLDGLIESIGLETKAPNGGLCV
                     AYFDGHYPTPLYDYEEEYLRSLEEKTSFYIQKVK"
     gene            6295..7317
                     /locus_tag="VGT87_001241"
     CDS             6295..7317
                     /locus_tag="VGT87_001241"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEQ3814095.1"
                     /translation="MSEKNAYAKSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVREPVLVSGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNNPVKFEEVVSGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQLIDGSKVKEGDILLGLASSGIHSNGYSLVRRVFADYTGKELLPELEGKQLK
                     DVLLEPTRIYVRAALPLIKEELVNGIGHITGGGFIENVPRMFADDLAAEIDEDKVPVL
                     PIFKALEKYGDIKHEEMFEIFNMGVGLMLAVSPENVNRVKELLDEPVYEIGRIIKKAD
                     DSVVIK"
     gene            7485..8039
                     /gene="purN"
                     /locus_tag="VGT87_001242"
     CDS             7485..8039
                     /gene="purN"
                     /locus_tag="VGT87_001242"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEQ3814096.1"
                     /translation="MKIAVFASGNGSNFQVIAEQFPVSFVFSDHRDAYVLERAQNLAI
                     PSFAFELKEFENKAAYEQAIVDLLDKHEIDLVCLTGYMKIVGETLLLAYEGRIINIHP
                     AYLPEFPGAHGIEDAWEAGVDQSGVTIHWVDSGVDTGQVIQQVRVPRLADDSLESFET
                     RIHETEYQLYPAVLDSLGVERKVI"
     gene            8223..9770
                     /gene="purH"
                     /locus_tag="VGT87_001243"
     CDS             8223..9770
                     /gene="purH"
                     /locus_tag="VGT87_001243"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEQ3814097.1"
                     /translation="MTKRALISVSDKSGIVDFAKELKNLGWDIISTGGTKVALDDAGV
                     ETIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDADSHLQAAKDNNIELIDLVVVNL
                     YPFKETILRPDITYDLAVENIDIGGPSMLRSAAKNHASVTVVADPADYATVLGELADA
                     GQTTFETRQRLAAKVFRHTAAYDALIAEYFTTQVGEAKPEKLTITYDLKQAMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDVDAAIRIIRDFKDRPTVVALKHM
                     NPCGIGQADDIETAWDYAYEADPVSIFGGIVVLNREVDAATAKKMHPIFLEIIIAPSY
                     SEEALAILTNKKKNLRILELPFDAQAASEVEAEYTGVVGGLLVQNQDVVAENPSDWQV
                     VTDRQPTEQEATALEFAWKAIKYVKSNGIIITNDHMTLGLGAGQTNRVGSVKIAIEQA
                     KDHLDGAVLASDAFFPFADNIEEIAAAGIKAIIQPGGSVRDQDSIDAANKHGLTMIFT
                     GVRHFRH"
     gene            complement(9829..10953)
                     /locus_tag="VGT87_001244"
     CDS             complement(9829..10953)
                     /locus_tag="VGT87_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEQ3814098.1"
                     /translation="MKKFHRFLVSGVILLGFNGLVPTMPSTLISQQENLVHAAVLGDN
                     YPSKWKKGNGIDSWNMYIRQCTSFAAFRLSSANGFQLPKGYGNACTWGHIAKNQGYPV
                     NKTPSIGAIAWFDKNAYQSNAAYGHVAWVADIRGDTVTIEEYNYNAGQGPERYHKRQI
                     PKSQVSGYIHFKDLSSQTSHSYPRQLKHISQASFDPSGTYHFTTRLPVKGQTSIDSPD
                     LAYYEAGQSVYYDKVVTAGGYTWLSYLSFSGNRRYIPIKEPAQSVVQNENTKPSIKVG
                     DTVTFPGVFRVDQLVNNLIVNKELAGGDPTPLNWIDPTPLDETDNQGKVLGDQILRVG
                     EYFTVTGSYKVLKIDQPSNGIYVQIGSRGTWVNADKANKL"
     gene            11206..12471
                     /gene="purD"
                     /locus_tag="VGT87_001245"
     CDS             11206..12471
                     /gene="purD"
                     /locus_tag="VGT87_001245"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEQ3814099.1"
                     /translation="MKLLVVGSGGREHAIAKKLLASKGVDQVFVAPGNDGMTLDGLDL
                     VNIVVSEHSRLIAFAKENEISWAFIGPDDALAAGIVDDFNSAGLRAFGPTKAAAELEW
                     SKDFAKEIMVKYNVPTGAYGTFSDFEKAKAYIEEQGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAQEMLLDNKFGDSGARVVIEEFLDGEEFSLFAFVNGDKFYIMPTAQDHKRAF
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVETIVRPVLEGMIAEGRPYLGVLYAGLILT
                     ADGPKVIEFNSRFGDPETQIILPRLTSDFAQNIDDIMMGIEPYITWQNDGVTLGVVVA
                     SEGYPLDYEKGVPLPEKTDGDIITYYAGAKFAENSELLLSNGGRVYMLVTTEDSVKAG
                     QDKIYTQLAQQDTTGLFYRNDIGSKAIRE"
BASE COUNT         3607 a         2442 c         2934 g         3585 t
ORIGIN      
        1 ctttttttaa aaaaacttat aaacgtaaat aatatcttga atatttttat agagtatgtt
       61 actcttaaat gaaaacagaa agaaaggcga ataatcaagt gactaaccag cttatttata
      121 aaggaaaagc gaaagatatt tatagtacaa aagatgaaaa tgtgattaga acggtctata
      181 aagaccaagc tactatgttg aatggagctc gtaaggagac tattgatggc aagggagcac
      241 taaataatca gatttcatct cttatttttg agaaattaaa taaggctggt gtggtgacac
      301 attatattga gcaaatttca aaaaacgaac aattgaataa aaaagttgat attattccac
      361 ttgaggtggt gttacggaat gtgatagcag gctcattttc aaaacgtttt ggagtggaag
      421 aaggacgtgt tcttgaaacg ccaattgtgg aattttacta caaaaatgat gatctgaatg
      481 atccttttat caatgatgag catgtgaagt ttttgggtat tgttaatgat gaggagattg
      541 cttatctgaa aggagagact cgccgtatta atgaactctt gaaaggttgg tttgctcaga
      601 ttgggcttaa cttgattgac ttcaagttgg agttcggatt tgatcaagaa ggtacaatta
      661 tcttagcaga tgaattttcg cctgataact gtcgcctatg ggataagaat ggtaaccata
      721 tggacaagga tgtttttaga agagaccttg gcaacctgac ggatgtctat caagttgtac
      781 ttgagaagtt gatagcattg taactagaag aggaaggaat tgaatccgtt cgggaaactc
      841 cgaaaaaaag atggaactta ctgaaatctc aatttcagat gtcctcctat tttccagtcg
      901 ctcccttgtc ggacttggta tcttatatga ataaacgtat ttttgttgag aaaaaggctg
      961 actttggtat taaatcggct agtcttgtga aagagttgac gcataatcta caactgacct
     1021 ctttgaaggc tttgcgtatt gtgcaggtct atgatgtctt caatttggct gaggatttgc
     1081 tggcgcgtgc tgagaagcat attttctctg agcaggtgac agactgtctt ttgacggaaa
     1141 ctgaaatcac tgcggagctt gataaggttg ccttctttgc cattgaggcg cttcctggtc
     1201 aatttgacca acgtgctgct agttcgcaag aagctttgct attatttgga agtgacagtc
     1261 aggttaaggt caatacagcc cagctttact tggtcaataa ggatattaca gaagcagagc
     1321 ttgaagccgt taagaactat cttttgaacc ctgttgattc gcgtttcaag gacattactt
     1381 tgccgcttga agagcaggct ttctctgtgt ctgataagac gatccctaat cttgatttct
     1441 ttgaaactta tcaagctgac gattttgcga cttataaggc agagcagggc ttggctatgg
     1501 aggtcgatga ccttctcttc atccaagatt atttcaaatc aatcggacgt gtgccaactg
     1561 agactgagtt gaaagttttg gatacttact ggtcagacca ctgccgtcac acaacctttg
     1621 aaactgaatt gaagaatatt gatttttcag cttctaaatt ccaaaaacaa ttgcagacga
     1681 cttatgacaa atatatcgcc atgcgtgatg agcttggccg ttctgaaaag ccacaaacac
     1741 ttatggatat ggcgactatt tttggtcgtt atgagcgtgc caacggtcgt ctggacgata
     1801 tggaagtctc agatgaaatc aatgcctgct cagttgagat tgaagtagat gttgatgatg
     1861 tgaaagagcc ttggctcctc atgtttaaga acgagactca caatcaccca acagaaattg
     1921 agccattcgg tggagcggcg acttgtatcg gtggtgctat tcgtgaccca ttgtcaggac
     1981 gttcatacgt ttatcaggct atgcgtattt caggcgcagg cgatatcacg actccgattg
     2041 cggaaacacg tgctggtaaa ttgccacaac aagttatttc taaaactgcg gcgcacggct
     2101 attcttcata tggtaaccaa attggacttg cgacaactta tgtgcgtgag tacttccacc
     2161 ctggcttcgt agccaaacgc atggagcttg gagctgtggt cggagcggca cctaaggaaa
     2221 atgtggttcg tgaaaaacca gaagcaggtg atgtggtaat cttgcttggt ggtaaaacag
     2281 ggcgtgacgg tgtcggcggt gcgacaggtt catctaaggt tcaaacggtt gagtctgtgg
     2341 aaacagctgg cgcagaggta caaaaaggga atgccatcga agagcgtaag attcaacgtc
     2401 tcttccgtga tggtgaagtg actcgtctca tcaaaaaatc aaacgacttt ggtgctggtg
     2461 gtgtctgtgt tgccatcggt gaattggctg acggtcttga aattgatttg gacaaggtgc
     2521 ctcttaaata ccaaggtctt aatggtactg aaattgcaat ctcagaatct caagagcgta
     2581 tgtcagtcgt tgttcgtcca agtgatgtgg atgccttcat cgcagcctgc aacaaggaaa
     2641 atatcgatgc agtcgttgtt gcgaccgtta ctgaaaaacc aaatcttgtc atgacttgga
     2701 atggcgaaat catcgttgat ttggaacgcc gtttccttga taccaatggt gtccgtgttg
     2761 ttgttgatgt taaagtcgtt gacaaggact tgacagttcc agaagcacgc acaacatcag
     2821 cagagacact tgaagcagat acgcttaagg tcttgtctga cctcaaccac gctagtcaaa
     2881 aaggtcttca aactatcttt gactcatctg ttggtcgttc aaccgttaac cacccaatcg
     2941 gtggtcgtta ccaaatcaca ccgacagaaa gttctgttca aaaattgcca gttcaacatg
     3001 gtgtgacaac aactgcatct gttatggctc aaggttacaa tccttatatt gcagagtggt
     3061 caccttatca cggtgctgcc tatgctgtca ttgaagcgac agctcgcttg gtagcaacgg
     3121 gtgctgactg gtctcgtgca cgtttctctt accaagagta ctttgagcgt atggataaac
     3181 aggcagagcg ttttggtcag ccagtatcag ctcttcttgg ttctattgag gctcagattc
     3241 aacttggttt gccatcaatc ggcggtaagg actctatgtc tggtactttc gaagacttga
     3301 cagtaccacc aaccttggta gctttcggcg tgacaacagc ggacagccgc aaggttctct
     3361 ctcctgagtt taaagcggct ggcgaaaaca tttactatat cccaggtcaa gctatttcag
     3421 aagatattga ttttgacctt atcaaggata actttagcca gtttgaagct attcaagctc
     3481 aacataagat tacagctgcc tcagccgcta aatacggtgg tgtcctagaa agtcttgctc
     3541 tcatgacttt tggtaaccgt atcggtgctt ctgttgaaat tgcagagctt gacagcagct
     3601 tgacagctca actcggaggt tttgtcttta catcagctga ggaaattgct gacgcggtga
     3661 aaatcggtca aactcaggca gactttacag tcactgtcaa tggaaatgac cttgctggcg
     3721 ctggccttct agcagccttc gaaggcaaat tggaagaggt ttacccaaca gaattcgaac
     3781 aggcagacgc tcttgaagaa gttcctgctg tggtatcaga tgctgttatc aaggctaagg
     3841 aaacaattga aaaaccggtg gtttacattc cagtcttccc tggtaccaac tcagagtacg
     3901 attcagctaa ggcctttgaa caggttggat ctagcgtcaa cttagtacca tttgtaacct
     3961 tgaatgaggc tgctattact gaatcagttg acactatggt tgctaatatt gctaaggcaa
     4021 atatcatctt ctttgctgga ggtttctcag cagcagatga accagatggc tctgctaagt
     4081 ttatcgtcaa tatcttgctt aacgagaagg tgcgcgcagc tattgacagc ttcatcgaaa
     4141 atggaggcct tatcatcggt atctgtaatg gtttccaagc ccttgttaaa tcaggtcttc
     4201 ttccatacgg aaacttcgaa gaagctggtg agacaagtcc aactctcttc tataacgatg
     4261 cgaaccaaca cgttgccaag atggttgaga ctcgtatcgc aaataccaac tcaccttggt
     4321 tggcaggagt tgaggtcggc gatattcatg ccattccagt ttcacatggt gaaggtaaat
     4381 ttgttgtcag cgcttctgaa tttgcagagc taagagacaa tggtcaaatc tggagccaat
     4441 atgtggactt tgacggacaa ccatctatgg attctaaata caatccaaac ggctctgtca
     4501 atgccatcga agggattacc agcaagaatg gtcaaatcat cggtaagatg ggacactcag
     4561 aacgctggga agacggactc ttccaaaata tccctggtaa caaagaccaa accctctttg
     4621 caagtgctgt aaaatacttt acagggaagt aaaaaggttc agtgaacctt tttagccctc
     4681 gccttgaatt agtgaagggc gaggttagaa agaatctcgc ttaatttttg agaaatagta
     4741 aacacgggcg ataagttgtg cgaaaaactt tctattttcg ctttacttga cagcctttgt
     4801 atcgaggtaa aaatgacata cgaagtaaaa tctctaaatg aagaatgtgg agtctttggt
     4861 atctggggac atcctcaggc agctcaagtc acttactttg ggcttcatag ccttcaacat
     4921 cgcggtcaag aaggagctgg tattgtttca aatgacaatg ggaaactcta tggttatcga
     4981 aatgttgggc tcctttctga agtttttaag aatcaatctg aattagataa tttaactggg
     5041 aatgcggcta ttggacatgt tcggtatgct actgcaggtt ctgcagatat tcgcaatatt
     5101 cagccttttc tttataaatt tcatgacggg caatttgctt tatgccataa tggtaatttg
     5161 acaaatgcta tttccttaag gaaagaatta gaaaagcaag gtgcaatttt caatgcctcc
     5221 tcagatactg aaattttaat gcatttgatt cgtcgaagcc ataacccaaa tttcatagga
     5281 aaggtaaaag aagctctaaa tacagtaaag ggtggctttg cctatctact gatgacagaa
     5341 aataaactca ttgctgctct tgaccctaat gcctttcgtc ctttgtcaat tggacaaatg
     5401 caaaatggtg cctgggttat ttccagtgag acctgtgctt ttgaggtggt aggcgcaaaa
     5461 tgggttagag atgttgaacc tggcgaagtt attcttatag acgatagtgg tattcaatgt
     5521 aatcgttata ctgatgaaac gcaactggca atttgttcga tggaatatat ctattttgca
     5581 aggccggatt caactataca tggtgttaat gttcatacgg ctcgaaaaaa tatgggaaag
     5641 catcttgcac aagaatttaa acaggatgct gatattgtaa ttggtgtccc aaattcgtcc
     5701 ttatcggctg ctatgggctt tgctgaagaa tccggattac caaatgagat gggtcttgta
     5761 aaaaatcagt atacgcagcg aacctttatt caaccgacac aagaattaag ggaacaaggt
     5821 gttcgaatga aactatcagc ggtatctggt gttgtcaaag gaaagcgcgt tgttatgatt
     5881 gatgactcaa ttgtaagagg aacgacttct agaaggattg tcggattatt aagagaagca
     5941 ggagctagtg aagtacatgt tgctatagct agtccagaat taaagtatcc ttgtttttat
     6001 ggtattgata ttcaaacaag acgagaatta atttcagcaa atcacgctgc tgacgaagtg
     6061 tgtgatatta ttggcgcaga tagtctgacc tatctctcac tagatgggtt gattgaatca
     6121 attggactcg aaacaaaagc accaaatggt ggtttatgcg tagcttactt tgatggacat
     6181 tatccaacac cgctttatga ttatgaagaa gaatacctca gaagtttaga agaaaaaaca
     6241 agtttttata ttcaaaaagt gaaataagct ctgctaatta tataaggaga aaacatgtct
     6301 gaaaaaaatg cttatgcaaa atctggtgtt gatgttgaag ctggttatga agtggttgaa
     6361 agaataaaaa aacacgttgc tcgtacagaa cgtgcgggag tcatgggagc tctaggtggc
     6421 tttggtggga tgtttgactt gagtaaaaca ggagttagag agcctgtttt ggtctcaggg
     6481 acagacggtg ttggcacaaa actcatgctt gctatcaagt acgacaagca cgacacaatc
     6541 ggtcaagact gtgttgccat gtgtgtcaat gatattattg cagcaggtgc tgagcccctt
     6601 tacttccttg actacgtagc gacaggaaaa aataatccag tcaaatttga agaagttgtt
     6661 tctggcgttg cagaaggttg tgttcaagca ggagcagcac tcattggagg tgagaccgct
     6721 gaaatgcctg ggatgtatgg cgaagatgat tatgaccttg ctggctttgc tgttggtgtg
     6781 gctgaaaaat ctcagctcat cgacggttca aaagtaaagg aaggcgatat tcttttggga
     6841 cttgcttcaa gtggtatcca ttcaaacggt tattctttgg tacgtcgtgt ctttgctgac
     6901 tatactggta aagagctgct tccagaactt gaaggaaaac aacttaagga tgtccttctt
     6961 gagccaactc gtatttatgt tagagcagct ctgccattaa tcaaggaaga actagtcaac
     7021 ggtatcggcc acatcacagg tggtggcttt atcgagaatg ttcctcgtat gtttgcagat
     7081 gatttggctg cggaaatcga tgaggacaag gtgccagtgc ttccgatttt caaggcgctt
     7141 gaaaaatatg gtgacatcaa gcacgaagaa atgtttgaaa tcttcaatat gggtgtcggt
     7201 cttatgctag ctgttagtcc tgaaaatgtt aaccgcgtca aagagctctt ggatgaacca
     7261 gtttatgaaa tcggtcgtat catcaagaaa gcagacgata gcgtggtgat taaataagtc
     7321 actaaatttt ataagaaagg agtgtttagt tagtttgcct aactgaacac gggattaaat
     7381 ttctaggaaa aaagataaaa tgacttagac gcaagcgtcg ttgttcgttt tcctattttt
     7441 ctacgaaatt tttcggcaag ccgaatcatc cctttgtatc gaatatgaaa atcgctgttt
     7501 ttgcttctgg taatggttcc aactttcagg tcatagcaga gcagtttcca gttagttttg
     7561 tcttttcaga tcatcgtgat gcctatgttt tagagcgtgc tcagaattta gccattccaa
     7621 gcttcgcttt tgaactcaag gagtttgaga ataaggcggc ttacgagcaa gccatcgttg
     7681 atttgttgga caaacacgag attgatttgg tctgtctaac gggctatatg aagattgttg
     7741 gagaaacctt actcttggcc tatgaggggc gtattatcaa tattcaccca gcctacctgc
     7801 ctgaatttcc aggtgcccac ggtatcgagg atgcttggga agctggtgtt gaccagtctg
     7861 gcgtgaccat ccactgggtt gactctggtg tggataccgg tcaggtcatc caacaagtgc
     7921 gcgtgccacg cctagcagac gatagcctag aaagctttga aacccgtatc catgaaaccg
     7981 aatatcaact ctacccagct gtcttagata gcttgggagt ggagaggaaa gtaatctagc
     8041 ctttatctta aaattttagg taaagttagg aaagatagta aagaagaatt aacgttccaa
     8101 aaagaaagga attgaactcg tctggaaggc acagaaaaaa gataaacatc tcctaggcgc
     8161 aagcgtcttc gtcgagtttc ctatttttcc ttggccttct agtcggactt ggtatcttaa
     8221 atatgactaa acgtgcttta atctcagtgt ctgacaagtc aggaattgtt gactttgcaa
     8281 aagaattgaa aaacttgggt tgggatatta tctcaactgg tgggactaag gttgcccttg
     8341 acgatgctgg tgttgagacc attgccatcg acgatgtgac tggattccca gagatgatgg
     8401 acggtcgtgt taagaccctc cacccaaaca ttcacggtgg gcttttggct cgtcgcgacg
     8461 ctgacagcca ccttcaggct gctaaggaca acaatatcga gctgattgac ctcgtggttg
     8521 tcaacctcta tcccttcaag gagactatcc ttcgtccaga cataacctat gatttggcgg
     8581 tggaaaacat cgacatcggc ggtccatcaa tgcttcgctc agccgctaaa aatcacgcta
     8641 gcgtgaccgt tgtggctgac ccagcggact atgcgactgt tttgggagaa ttggctgacg
     8701 ctggtcagac gacatttgaa acgcgtcaac gcttggcagc taaggtcttt cgtcacacag
     8761 cagcctacga cgctttgatt gctgagtact tcacaactca agtgggagag gctaagcctg
     8821 aaaaattaac catcacttac gaccttaaac aggctatgcg ttacggagaa aatccacaac
     8881 aagacgctga tttctatcaa aaagccttgc caacagacta ctcaattgct tctgctaaac
     8941 agctcaacgg aaaagaattg tccttcaata atatccgtga tgttgatgca gcaatccgta
     9001 ttatccgtga tttcaaagac cgtccgacag tggttgccct caaacacatg aacccatgtg
     9061 gtatcggaca ggctgatgat attgagactg cttgggatta cgcttatgaa gctgacccag
     9121 tttcaatctt tggtggaatt gtcgtcctta accgtgaagt tgacgcagcg acagccaaga
     9181 agatgcaccc tatctttttg gaaatcatca tcgcaccatc atactcagaa gaagcgctag
     9241 ctattctcac aaataaaaag aaaaacttgc gaatccttga gttgccgttt gatgcccaag
     9301 ctgccagcga agtggaagct gagtacactg gcgtagttgg tggacttttg gtgcaaaacc
     9361 aagacgttgt ggctgaaaat ccatctgact ggcaagtggt gacagaccgc caaccaacag
     9421 aacaagaggc gactgccctt gagtttgcct ggaaggctat caagtatgtt aagtctaacg
     9481 ggattattat tactaacgat cacatgacgc ttggactcgg tgcaggtcaa accaaccgtg
     9541 tcggctcagt caagattgct atcgagcagg ctaaagacca ccttgacggt gccgttctag
     9601 catcagatgc cttcttccca tttgcggaca acattgaaga aatcgctgcc gcagggatca
     9661 aagcaatcat ccaaccaggt ggttcagttc gtgaccaaga ctctattgac gccgcaaaca
     9721 aacatggctt gaccatgatc ttcacaggtg tgagacattt taggcattaa taaaaaaatg
     9781 tggtctaatt aaaaccacat ttttttatta aaagaaaata taagttaact ataatttgtt
     9841 agctttatca gcatttaccc atgttccacg agatccgatt tgaacataaa taccattact
     9901 tggttgatca atttttaata ctttataact accagtgacg gtaaaatatt cacccacacg
     9961 gagaatttga tctcctaaaa cttttccttg gttatctgtt tcatctaatg gtgtgggatc
    10021 aatccagttt agtggagttg ggtctcctcc ggctaattct ttattaacga tcaaattatt
    10081 aacaagctga tctacacgaa aaacgccagg gaaggtaaca gtatcaccga ccttaatgga
    10141 aggttttgta ttttcatttt gaaccacaga ctgtgcgggc tctttaatgg gaatatagcg
    10201 tcggtttcca gaaaaactga ggtagctaag ccatgtataa cctccagcag tcacgacttt
    10261 atcgtaataa acagattgac ctgcttcata gtaagcaaga tcagggctat cgatactggt
    10321 ttgtcctttg actggtaatc tggttgtaaa gtgataagtt ccagaggggt caaatgaagc
    10381 ttgagaaatg tgttttagtt gtcttgggta ggaatgactt gtctgagatg ataagtcttt
    10441 aaaatggata taaccactta cctgagattt tggaatttga cgcttatggt atctttcagg
    10501 gccttgtcca gcgttgtaat tatactcttc gatagtgaca gtgtctccac ggatatcagc
    10561 tacccatgct acatgaccgt aagcagcatt tgactgataa gcgtttttat caaaccaagc
    10621 gatagcccct atgcttggtg tcttattcac aggataaccc tgatttttcg cgatatgtcc
    10681 ccacgtgcag gcattaccgt agcctttagg taactgaaaa ccattagcag agcttaaacg
    10741 aaaagctgca aaagaagtgc attggcggat atacatgttc cacgaatcga ttccattgcc
    10801 ttttttccac ttactcggat agttatcgcc taaaacagct gcatgaacaa gattttcctg
    10861 ttgcgaaata agtgtagatg gcatagtagg tactagacca ttaaaaccta aaaggattac
    10921 tcctgagacc aaaaaacgat gaaatttttt cattaattac ctcctgtaac ttattattcg
    10981 aaagcaagaa acaaaaaatg ttatttttag atgattattt tgaaaattgg ctttacttga
    11041 tattaaataa ctgtttgatt ggctaataga gtgttcctta tttcaagaat tgtggtctac
    11101 tatcacattt ttacaatgtt tcaattattg ataattaata cattgtataa aaaggcgaac
    11161 gtttatgata taatatctaa ttataatacg aaagaggtgg atgttttgaa attacttgtt
    11221 gttggttcag gtggtcgtga acatgcgatt gctaagaagt tgttagcgtc taagggtgtg
    11281 gatcaggttt ttgtggcacc tggtaatgat ggtatgacct tggatggtct ggacttggtg
    11341 aatatagtag tttccgaaca ttccaggttg attgcctttg ctaaggagaa tgagatttct
    11401 tgggccttta ttggtcctga tgatgcgcta gcagctggta tcgttgatga tttcaacagt
    11461 gctggactca gagcttttgg tccaaccaag gcagcagcgg agctagagtg gtcaaaagac
    11521 tttgccaagg aaatcatggt caaatacaat gtcccaacag gagcctatgg cacattttca
    11581 gactttgaaa aagccaaagc ctacatcgaa gagcagggcg cacctatcgt ggtcaaggct
    11641 gatggcttgg cgctaggtaa gggtgtggtc gtggctgaaa ccgttgagca ggcggtagag
    11701 gcggcgcaag agatgcttct ggacaataag tttggcgatt caggagcgcg tgtggttatc
    11761 gaggaattct tggatggcga ggagttttcc ctctttgcct ttgttaatgg tgataagttc
    11821 tacatcatgc cgacagctca ggatcacaag cgtgcctttg acggagacaa ggggccaaac
    11881 actggtggta tgggtgccta tgcgccagtt cctcatctac ctcagagcgt ggtggataca
    11941 gcggttgaga cgatcgttag gcctgtcctt gagggtatga ttgccgaagg gcgtccttat
    12001 ctaggcgtcc tctatgcggg gcttatcctg acggctgatg gtcctaaggt tatcgagttc
    12061 aactcacgtt tcggtgaccc tgaaactcag attatcctcc ctcgcctgac ttccgatttc
    12121 gctcagaata ttgacgatat catgatgggc attgagcctt acatcacttg gcagaatgac
    12181 ggtgtgactt tgggtgttgt cgttgcctca gaaggctatc cgctcgatta cgagaaaggt
    12241 gtgccactgc ctgaaaagac cgacggcgat atcatcacct actatgccgg agctaagttt
    12301 gcggaaaata gcgagctact tctctcaaac ggaggacgtg tctatatgct cgtcaccaca
    12361 gaagacagcg tcaaagcagg gcaggacaaa atctataccc aactggccca acaagacaca
    12421 acaggtctct tctaccgaaa tgacatcgga agcaaagcta taagagaata agactaatca
    12481 ttcaaatatt agcgtcagca atcagcgacc gaaggtcgct ccaaaaagaa agaagaaagg
    12541 aactgaacac gggactaatt tcctagga
//
LOCUS       DAUCLR010000054        12567 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.054, whole genome shotgun sequence.
ACCESSION   DAUCLR010000054 DAUCLR010000000
VERSION     DAUCLR010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12567
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.054"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(19..750)
                     /locus_tag="VGT87_001246"
     CDS             complement(19..750)
                     /locus_tag="VGT87_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEQ3814100.1"
                     /translation="MRLDKFLVATGVGTRSQVKLLLKKKAIFVNQKVETSAKAHIDEY
                     KDLVTYQGTPLVYESFVYYLLNKPSGYVSATQDRQQATVMELLDDTARQKAVFPVGRL
                     DKDTRGLLLLTNNGQLAHDLLSPKKHVTKEYLAKVVGIMTEADKDYFARGISLKDHQC
                     LPAHLEVLASDLQQQTSLVKITIQEGKFHQVKRMVAACGKGVLDLQRLSMGPLKLDPS
                     LAEGEFRRLTPEELQSLAPYCQSSL"
     gene            865..1230
                     /locus_tag="VGT87_001247"
     CDS             865..1230
                     /locus_tag="VGT87_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEQ3814101.1"
                     /translation="MTIYSHTYETRPNHSAKAMGSGTLNVLATPALVAFMENASYLFA
                     QEQLDSDFTTVGSEIAIQHLAASAIGDPVTIIITALKEEGRKYDFRLEAFVGEQLIGK
                     ACHTRVRVNSKHFMAKVAE"
     gene            complement(1350..2570)
                     /locus_tag="VGT87_001248"
     CDS             complement(1350..2570)
                     /locus_tag="VGT87_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ3814102.1"
                     /translation="MSTLSLDTTNKRALVAAIAASGTDDLNVMFLAFSMSSIMTDLSL
                     SGTQGGWIATITNLGMLVGGLLFGLLADRHHKFKVFKWTILLFSVATGLIYFTQSLPY
                     LYLMRFIAGIGVGGEYGVAIAIMAGIVPPEKMGRMSSLNGIAGQLGSISSALLAGWLA
                     PSLGWRGLFLFGLLPILLVIWMTLAIDDQKIWDHYGQEEEECSQPIKINELFKTKSLT
                     AQTLALMVMTTVQIAGYFGMMNWLPTIIQTSLNLSVKSSSLWMVATIVGMCLGMLCFG
                     QLLDCFGPRLIYSLFLLASSICVYLFQFANSMASMVIGGAIVGFFVNGMFAGYGAMIT
                     RLYPHHIRSTANNVILNVGRALGGFSSVAIGSILDASGISMVMIFLASLYVISFGAMW
                     SIGQLKAERYQQLR"
     gene            2944..4428
                     /locus_tag="VGT87_001249"
     CDS             2944..4428
                     /locus_tag="VGT87_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEQ3814103.1"
                     /translation="MKTIAFDSNKYLNLQRDHILERISQFDGKLYMEFGGKMLEDYHA
                     ARVLPGYEPDNKIKLLKELKEQVEIVIAINANNIEHSKARGDLGISYDQEVFRLIDKF
                     NTLDIYVGSVVITQYNNQPAADAFRKQLEKNGIASYLHYPIKGYPTDINHIISSEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSQMYHDQINGVKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLETYGKTAVNYNRDIEVFPVLNRTFERILSKSPYA
                     SPTDMGVNMVGFSIVNEEAAIEASKQEIIRRYYQTLVDFKAERVTESAVKKIELLMND
                     IGVTPDDRHVTVAAHQKAEQTGQPALALQLPNGQIVTGKTSELFGPTAAVIINAIKTL
                     AKIDKTTHLIEPEYVKPIQGLKVNHLGSHNPRLHSNEILIALAITAMTSEEANLAMKE
                     LGNLKGSEAHSTVILTEEDKNVLRKLGVNITFDPVYQHHKLYRK"
     gene            complement(4533..4934)
                     /locus_tag="VGT87_001250"
     CDS             complement(4533..4934)
                     /locus_tag="VGT87_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEQ3814104.1"
                     /translation="MSDLTQEMTLNVISIFDNYQIELAEKGHLILSTEVTETALNYYG
                     NAHGGYLFTLCDQVGGLVARTTGVESVTLQANANYLKAGHKGDKLMVEGRLVHGGRTT
                     QVVDVTIHNQTGALLTKTSLTMFVTGRRHNG"
     gene            complement(5019..5800)
                     /locus_tag="VGT87_001251"
                     /pseudo
     CDS             complement(5019..5800)
                     /locus_tag="VGT87_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060407.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
     gene            complement(5965..7737)
                     /locus_tag="VGT87_001252"
     CDS             complement(5965..7737)
                     /locus_tag="VGT87_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEQ3814105.1"
                     /translation="MNTKVKLIKASNTGARNRNALHLDLKRVAAYCRVSTDSKDQLES
                     YKSQVDYYTNLIKNNKNWTLAGIYADEATTGTTATKRADFMRLISDCQNGDIDMIITK
                     SISRFARNTLDTLKYVRLLKENNVGVVFEEENIDTLTMDGELLLTILSSVAQQEVENT
                     SAHVKKGLKMKMEKGELIGFQGCLGYDYDPVTKSISINEEEAKIVRYIFKRYLEGNGG
                     SVIGRELEEQGYLTPRGKTKWSDTTVLGIIKNEKYIGDILMGKTFTVDPITKRRLANF
                     GESDKYHIENHHEPIISREDFEKAQEIRLRRAQNRNTIANKDRKREKLSRQYAFSSML
                     ECGFCGEILSRRTWHTSSIYKKINWQCVKSTKKGKKYCPHSKGIQEAAIEKAFVESYR
                     QLCHADSTVIDDFLKIVEEEINDNTLVKDLKKIENQLNRIISQERKLVDLHLEDSIDE
                     EVYAKKYKKLTKQKEELLDEKKTLELTIKDEDSIKERLKQFKKVLENKEIIEEFNGTV
                     FESIVDKVVVGRIDKDGTVHPYDLTFYFKTGVKDSQDSNNFKDKRKNAKDNDINKLCS
                     YKNDEDKKLCSQSKDNARGVCKFT"
     gene            complement(7793..7930)
                     /locus_tag="VGT87_001253"
     CDS             complement(7793..7930)
                     /locus_tag="VGT87_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012797125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814106.1"
                     /translation="MKAIKVYSMRVAMLCRLYDLKLINDKEYTKIKNRIENDYKKRDR
                     K"
     gene            complement(8468..8878)
                     /locus_tag="VGT87_001254"
     CDS             complement(8468..8878)
                     /locus_tag="VGT87_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEQ3814107.1"
                     /translation="MDKEYYLFVEGKKVFVSKEVYLAYHSELNKEKYQTRRDRLNNCF
                     FFCSYDHDGNFEENLEDLEFDVEKIIETKEMIEEVRRAISKLNPAERELIESLFYKEE
                     TIREVAAKLNISHPAVIKRRNKVLEKLKEMLENF"
     gene            complement(9148..9663)
                     /locus_tag="VGT87_001255"
     CDS             complement(9148..9663)
                     /locus_tag="VGT87_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814108.1"
                     /translation="MYADSNLKFEKISSAAKLILSLYRQVTWAVDSRANFMMFESKEN
                     YGSTSESAYLYLSTFAPEKVEEKFNNRVSNVMDSKLMLLIIYDACVRLKVYPEYGEIY
                     HKIIYNYYISEKKITDEACMRSASLERTVYYQRKKEAIALVGVIIWGYTLPTAISQLE
                     DGRSIEEIMNI"
     gene            9858..10259
                     /locus_tag="VGT87_001256"
     CDS             9858..10259
                     /locus_tag="VGT87_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ3814109.1"
                     /translation="MAFADRLKDFREKEKLSQADFAKMIGISTRTLVHYEDGERYPRD
                     VEVYKKIAEVMDCDYNYLLEESDEFLNRVYNMGGKRELEKARALTEGLSSLFAGGEIS
                     DEDKDAAFEAITRAYWEAKRENKKYGRKKKD"
     gene            10268..10816
                     /locus_tag="VGT87_001257"
     CDS             10268..10816
                     /locus_tag="VGT87_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004818771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEQ3814110.1"
                     /translation="MSMNRYIYDKVNRLIKKYKTRDPIELIEALNINLVYLPETKILL
                     GMYHYIQRNRFIFISSNTNFNRKTILAHELGHDQLHRDYCMSGGAFHDQTVLNPTNKF
                     ETEANIFAAHLLISDEDILDNINDQVCDYEIAGQLGVDINLLNLKISELAKMGKFNKP
                     VNVNIPQGDFLKNYRPEHDDYY"
     gene            10825..11307
                     /locus_tag="VGT87_001258"
     CDS             10825..11307
                     /locus_tag="VGT87_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814111.1"
                     /translation="MGIFITGYFLLILSGIVFYYLISTYSVKISAFLIDFIVVGLGFY
                     ISIKEKLDNKLAIPLSITVVIIYGILLVLINQKLPRISKLLNYIIAFIGSSVALWLAL
                     DFITNTLTAFKIIGQTYHQLPITKSMMINSFIHYVIVFLISIPVFKGRMKFILGGNYE
                     "
     gene            11300..12373
                     /locus_tag="VGT87_001259"
     CDS             11300..12373
                     /locus_tag="VGT87_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HEQ3814112.1"
                     /translation="MSKEMDKNKDENQEKNELQIRSSAAEYLTFIAANGDDQEAIEIR
                     YEDENIWLTQKMMAALYDVSVSAINQHLKKIFDDNELDESSVIKKYLITANDGKSYNT
                     KHYNLQAIIAVGFKVNNERAVQFRKWSNQIVKDFTIKGWAMDDERLKNSGTKLTKDYF
                     EKLLVKIREIRLSERRFYQKITDIYATSLDYDPSAKATKRFFAAIQNKLHYAIHGKTA
                     AELIVDRANHKTKNMGLTTWEGSPDSKIHKYDVVVAKNYLNEEELNQMQRIVSSYLDM
                     AEMQAERHIPMTMEDWEKRLNGFLQLWDKEILNDAGKVSAALAKKHAETEFEKYRIIQ
                     DRLYKSDFDKFLELEDDTKKLEE"
BASE COUNT         4038 a         2408 c         2041 g         4080 t
ORIGIN      
        1 gacaggggag gtttcttttt atagtgagct ctgacaataa ggtgccaggc tttgcaattc
       61 ctcaggcgtc aggcgcctaa actccccttc tgcaaggcta gggtctagtt ttaatggtcc
      121 catgctgaga cgctgcaagt caagcactcc ctttccacag gctgctacca tgcgtttcac
      181 ctgatggaat tttccttctt gtatggtaat tttaaccaaa cttgtttgct gctgtaaatc
      241 actagcaagc acttctaaat gagcaggtag gcactggtga tcctttaagg aaatacctct
      301 agcaaaataa tctttatcag cttcagtcat gatgcctact actttagcca agtactcttt
      361 cgtaacatgt tttttagggg ataaaaggtc atgggctagt tgtccattat tggtcaagag
      421 caaaagtcca cgggtatcct tatctaacct acccacagga aaaactgctt tttgcctagc
      481 cgtgtcatct aacaactcca taacagtagc ctgctgtctg tcctgggttg ctgacacata
      541 ccctgacggc ttgtttaaca ggtaataaac aaaggattca tagaccaagg gcgtgccttg
      601 atacgtcacc aaatccttgt attcatcaat gtgtgctttg gcagaggttt caaccttttg
      661 attgacgaaa atagccttct tttttaagag aagtttgact tggctcctag ttccaacacc
      721 agttgccact aaaaatttat ctaagcgcat cataaccctt tcaaagaaaa ttcattatta
      781 agtataacat aaaaaaacag tagtttcatg actgtaagcg cttaatatat tataataggc
      841 ttatagaaac gaggagatta acttatgact atttattctc acacttatga aacccgtccc
      901 aatcattcgg ctaaagccat gggctcagga acgctgaatg tcctagctac tcctgctctt
      961 gttgctttta tggaaaatgc ttcttactta tttgcccaag aacagctcga cagtgacttt
     1021 acaacggttg gcagcgagat agctatccaa catttggcag cttcagccat tggagatcct
     1081 gttacaatca tcattacagc ccttaaagaa gaaggccgaa aatacgattt ccgcttggaa
     1141 gcttttgtgg gagaacaact cattggaaaa gcctgtcata ctagagttag ggtgaacagt
     1201 aaacatttta tggccaaagt agcggagtaa aacaaaagaa aagaaccaaa aaggtaagca
     1261 atccctaagt gcttaccttt ttggttatct caatcaataa aatagctttg tgagtgaaag
     1321 taactcaaat actaacaccc atctctttgc taacgcaatt gttggtagcg ttctgctttt
     1381 aactgaccaa tagaccacat agctccaaaa ctaatcacat acagagaagc taggaaaatc
     1441 atgaccattg aaatacctga ggcatctaaa atactaccaa tagcaacgga cgaaaatcct
     1501 cctagagcac ggccaacatt caaaataaca ttattagccg tggatcgaat gtgatgggga
     1561 taaagtctgg taatcatagc cccgtagcca gcaaacatcc cattgacaaa aaaaccgaca
     1621 atagcacctc caataaccat actcgccatg gaattggcaa attgaaaaag gtaaacacaa
     1681 atagatgatg ctaataaaaa gagggaataa atcagacgtg gaccaaagca atctaaaagc
     1741 tgaccaaaac acagcatccc caaacacatg ccgacaatag tagccaccat ccacaaggac
     1801 gaacttttta ctgaaaggtt taagcttgtt tgaataatgg ttggcaacca attcatcatt
     1861 ccaaaatagc ccgcaatctg cacagtcgtc atgaccatta aagcaagggt ttgagctgtt
     1921 aaggattttg ttttgaatag ttcattgatt ttaattggtt gactgcattc ttcctcctct
     1981 tgcccataat gatcccaaat tttttggtca tctatggcta aagtcatcca aatcaccaag
     2041 agaatcggaa gtaatccaaa gagaaaaagg ccacgccagc ctagacttgg agccagccac
     2101 cctgctaaaa gagccgaact aatggagcca agctggccag caataccatt taaagaagac
     2161 atccgaccca ttttttctgg tggtacaatc cctgccatta tggcaatggc tacaccgtac
     2221 tcacctccaa ctcctatgcc agcaataaat cgcatcaggt acaggtaggg caaagactgg
     2281 gtaaaataaa ttaatcctgt tgctactgaa aacagtaaaa tcgtccattt aaagacctta
     2341 aacttgtggt gcctatcagc cagcaacccg aataagaggc ctcccaccaa cattcctaaa
     2401 ttggtaatgg tcgcaatcca gccaccctga gtgccactaa gactcaagtc tgtcataatg
     2461 gaagacatgg aaaaggcaag aaacatgaca ttcaagtcat ccgttcctga agctgcaata
     2521 gctgccacta aagctcgctt atttgtggtg tccaatgata atgttgacat aattgtctcc
     2581 ttcttaatag tagctcactg actaccttaa ttaattgaat accctttgtc caaaggtcaa
     2641 aaaaagactt ggaaacaagt ccgcaagtcc atcaaagttc actaaataat aagagatgac
     2701 aaaaaaactc tctttaatgt taagactttc atgcttcacg gaacatgttt aaagtagtat
     2761 ttaattgtta ctagtatagt aaagctcatt ttaattgtca agttcttttg aacatagcgg
     2821 atacattttt cttctaagaa ccaagcctaa gtttttttaa aaagtcacaa aatgaggtat
     2881 ccctgcaaaa aaccttttct ttcttggaat ttctttgtta taatgcttta cagaggtgaa
     2941 aaaatgaaaa ctattgcttt tgattctaac aagtatttaa acttgcaaag agatcatatt
     3001 ttagaacgta tcagccaatt tgatggcaaa ctttacatgg aatttggtgg taaaatgtta
     3061 gaagattacc atgctgcccg tgtacttcct ggctatgaac ctgacaataa gattaaatta
     3121 ctcaaagagc tcaaagaaca agtcgaaatt gtaattgcca ttaacgctaa taatattgaa
     3181 cattctaaag cgcgtggtga tttaggcatt tcttatgacc aagaagtctt tcgcttaatt
     3241 gacaaattta acacgttaga tatttacgta ggttctgtcg ttattactca atacaataac
     3301 caacccgctg cagatgcctt tcgcaagcaa ctcgaaaaaa atggcattgc ttcttacctg
     3361 cattatccta tcaagggcta cccgacagat attaatcata ttatctcatc tgaggggatg
     3421 gggaaaaatg attacatcaa aacgagtcgt aaccttatcg ttgttacagc accaggccct
     3481 gggtctggaa aattagccac ttgcatgtcc caaatgtatc acgaccaaat caatggggtt
     3541 aagtctggct atgctaaatt tgaaaccttc ccagtttgga atcttcctct tcatcaccct
     3601 gtcaatctgg cttatgaggc agccacggca gacctagatg atgtgaatat gattgacccc
     3661 ttccatttgg aaacttatgg caaaacggcc gttaattaca accgtgatat cgaggtcttt
     3721 cctgtcttaa atcggacctt cgagcgtatt ttgagcaagt ctccttatgc ctcaccaacc
     3781 gatatgggtg ttaacatggt tggtttttct atcgtcaatg aagaagctgc tatcgaagct
     3841 tctaaacaag aaatcatccg tcgttattac caaaccctcg tggatttcaa ggcagagcga
     3901 gtcacagaat ctgctgtcaa aaaaattgag ctcttaatga atgatatcgg tgtcacccct
     3961 gacgatcgtc acgttactgt agctgctcat caaaaagcag agcaaacagg ccaacctgcc
     4021 cttgctctcc aactaccaaa tgggcagatt gtgacaggta aaacatctga actctttgga
     4081 ccaactgcag ctgttatcat caatgcgatt aaaaccttgg caaagattga taaaaccact
     4141 catttgattg aacccgaata cgtgaaacct attcaaggcc tcaaagtgaa ccacttaggc
     4201 agtcacaatc cgcggcttca ttccaatgaa attctgattg cgttggccat tactgctatg
     4261 actagtgaag aagctaatct agccatgaaa gaactcggca atttgaaagg tagtgaagcc
     4321 cattctaccg ttatcttaac cgaagaagat aaaaatgtac tccgaaaact tggggtaaat
     4381 attacctttg accctgttta ccaacatcac aaactttacc gtaaataaac cgtaaccatc
     4441 cgtctaacgg gtgatttatg gtatctctca ataaaggaaa aaagtcccaa ttctcagaga
     4501 gttgggactt ttttccttta ttgattcatt aatcacccgt tatgacgtcg gccagtcaca
     4561 aacatggtca agcttgtttt tgttagaaga gctcctgttt ggttatgaat cgtcacatca
     4621 accacctgcg tggttcgacc tccatgaacc aatcgacctt caaccatcag cttgtctccc
     4681 ttgtgccctg cttttaaata gttagcattg gcctgtaagg tgacagattc tacgccagta
     4741 gttctagcaa ccaaaccacc tacttgatca catagggtga aaagataacc accatgggca
     4801 ttcccataat aattcaaagc cgtctcagtc acttcagtac ttaaaataag gtgtcctttt
     4861 tcagcaagtt caatttggta attatcaaaa atactaataa catttaaggt catctcttgt
     4921 gttaaatctg acattgtttt tctcttttct aagcttagtc taaaagcaat acaagcctca
     4981 ttatactaca aataggaaca gaaggcagag ctcatgtttc aaaaatcagt agcctttcca
     5041 tccttttcta gcgtattgag cacttccaag cataaatgaa gcgcaaaact aaaggaatca
     5101 gcaccccagt tgcgttgatc gtaaacctct acatctgcta gagtatctgc tgtaaataag
     5161 agttgccccc aaaggatgcc tcttaattga gctactgctg ctaaggcaga acattccatc
     5221 tcaacaaccg aacacccttc ttcctggcga taagccactt tttctttggt ttctcggtaa
     5281 aaaccatctg ttgaccaggt gatgacctct tgataagcta acccctgtgc taaaagcgtt
     5341 ttctcaatca gtctaagcat cttaggatca atatcaatat aacgagatgg tgccacgtaa
     5401 tgataacttg ttccttcatc tcgcaaagcc ttaactggca ctaaaaaacg gttttctgcg
     5461 atagaaacca aaacgccaca ggttcctgta ctgatcattt ctttaacccc ataaccaatc
     5521 agccagtcca tgaattgcgc cgcaggagct gagccaacgg gagcttgagc taggcaaatg
     5581 tcttggcctt tataaggtat ctgataaaca gggtaaacct ttgtggctga aatgaattct
     5641 gctacttttt taccttgatg gtctaaagca aaagcttcaa tctcgtcttc taagaaagca
     5701 tagacacact tagctgtaga ccttcatgtg taggtataat gacggctgat ggattactat
     5761 cacattctaa aaggggaatc tcgtgtttgg taataggcat tcgtttctcc ttatttttgt
     5821 catgtggagg cagctacctt actccttgat tagcaacact acgcactcga cgtgatgcgt
     5881 ctgcgggagt caaaaggtcc agtggacctt ttgagcgtct caccttgaaa taacagagtg
     5941 aggcggactc tttaactccg tttctcaagt aaacttacac actccacgtg cgttgtcttt
     6001 tgattgggaa cataattttt tatcctcgtc attcttgtag gaacacaatt tattaatgtc
     6061 attgtctttg gcattttttc ttttatcttt aaagttatta gaatcttgac tatctttaac
     6121 tcctgttttg aaatagaatg ttaagtcata tggatgaact gttccgtctt tgtcaattct
     6181 ccccaccaca actttatcaa ctatgctttc aaatactgtt ccgttaaatt cctctataat
     6241 ttctttattt tctaagacct ttttaaattg ctttaatctt tctttaatag agtcttcatc
     6301 tttgatagtt agttccaatg ttttcttttc atcaagtaat tcttcttttt gttttgtaag
     6361 ttttttatac tttttagcat aaacttcttc gtctatacta tcttctaaat gaagatcaac
     6421 taactttctt tcttgactaa tgattctatt taattggttt tctatctttt tcagatcctt
     6481 gactaaagta ttatcattta tttcttcttc aacaattttt aaaaagtcat ctattactgt
     6541 tgaatctgca tggcataatt gcctatagct ttcaacaaat gctttttcta ttgctgcttc
     6601 ttgtattcct tttgaatgag ggcaatattt tttacctttt tttgttgatt ttacacattg
     6661 ccagtttatt tttttgtaaa ttgaacttgt atgccaagtt cttcttgaaa gtatttctcc
     6721 acaaaatcca cattcaagca tacttgaaaa agcatattgt cttgatagtt tttctctttt
     6781 tctatccttg ttagctatgg tgtttctgtt ttgtgctctc ctaagtctaa tctcctgtgc
     6841 cttttcaaaa tcttctcttg atataattgg ctcgtgatga ttttcaatgt gatatttatc
     6901 agattctcca aaatttgcta atcttctttt tgttatggga tcaactgtaa aggtctttcc
     6961 cattagtata tcgccaatat acttttcatt cttaattatt cctaacactg tagtatcaga
     7021 ccattttgtt ttacctcttg gggtaagata tccttgctct tctaattccc tgccaatgac
     7081 tgagccgcca ttaccctcta aatatctttt aaagatgtat ctaacaatct tagcttcttc
     7141 ttcatttata gagatacttt ttgttacagg gtcatagtcg tagccgaggc aaccttgaaa
     7201 accaataagt tccccttttt ccattttcat tttcagtcct tttttcacat gggctgaggt
     7261 attttctact tcttgttgag ctactgaact taatattgtt aatagtaact ctccatccat
     7321 tgtcaaagta tctatatttt cttcttcaaa tactacacca acattatttt cctttaaaag
     7381 ccttacatac tttaaggtat ctaatgtatt tctagcaaat cttgatatag atttagtaat
     7441 tatcatgtct atatctccat tttggcaatc acttattaac ctcatgaaat ctgctctttt
     7501 agtagcagtt gttcctgttg ttgcttcatc tgcatatata ccagccaaag tccagttctt
     7561 attatttttt attagatttg tgtaataatc aacttgcgat ttatatgatt caagttggtc
     7621 tttactatca gtgctaactc gacaatatgc tgcaactcgt tttaaatcta aatgtagtgc
     7681 atttctattt cttgctcctg tatttgaagc ttttattaat tttactttag tattcatttt
     7741 acctccttcc cattttgtat ctttctacag tgttattata tcacaagatt aactactttc
     7801 tatctctctt cttataatca ttttctattc tattttttat ttttgtgtat tccttatcat
     7861 taattaattt aagatcataa agtctacata acatagctac tctcatgcta taaactttta
     7921 ttgctttcat aataacctcc tatgtaaatt taagtttttt gacattgaca agtgccttgg
     7981 attagcaaca taggaatctc acctccgcct ctgttcagga tgagccggct ttaaccttag
     8041 aagtatcatt atcctcattt tcttcattgc gaatagggta tccctcccta tattcaaact
     8101 cttacttatc gctccctttc ttcttccctt gcttttagct tagcagtact tgttttgccg
     8161 aattattcag cagaaagatc ttggcggata ggttatcacg ctccaaaaat gattaacgtc
     8221 ttgtcttggt ctattcaatt gttaaggttc aaaatttatc gagagctatt aatcttttta
     8281 aaatttgacc atcagaaaat acttatcttg atgaaacaaa attgtttaaa attaccctct
     8341 tatacttaac agtgaaaaga agtctttctt ggtaaccaat tttcaaaaat aaatttgctt
     8401 aaataaaaaa gtccaattcc cactgtataa gtaacagtga aaattagacc ctcttggcaa
     8461 ctgtcatcta aaagttttcc aacatttcct ttagtttttc taagacttta ttacgccttt
     8521 taataacggc tggatgagaa atattcagtt tggcagctac ttctctaatt gtttcttctt
     8581 tataaaagag gctttctatt agctctcttt cagcaggatt gagtttagaa atagctcttc
     8641 ttacttcttc aatcatttcc tttgtttcga taattttttc aacatcaaat tccagatctt
     8701 ctaaattttc ttcgaagttt ccatcatgat cataggaaca aaaaaagaaa cagttattta
     8761 acctgtctct tctcgtttga tatttctctt tatttaattc actatgataa gcaagatata
     8821 cttccttgct aacaaatact ttcttccctt ctacaaataa ataatattct ttgtccatta
     8881 atttttcctc cttgttgaca tgaatttctt ttgtttgaaa atgaaattca cacaaggagg
     8941 gcttctaatt ttttgcatat attctcctgg gcataaaaaa agaccgaaag aaaataaatt
     9001 ctttcagtca ataaatgcca atattaaatt taggtgcaaa tcacctatca tggatattat
     9061 atatgcactt aagtacacat aaaattcgtg gatggtactt ttttcattcg gaaatagtac
     9121 gcaataagtt cgtcataaat taagtcctta gatgttcatt atttcttcaa tgctacgacc
     9181 gtcttcaagt tgactaatag cagtcggtaa agtatatccc caaattatta cacctaccaa
     9241 tgcaattgct tccttctttc tttgataata aacagttctc tctaaagacg cacttctcat
     9301 acaagcttca tcagtaatct ttttttcaga aatgtagtag ttataaataa tcttatgata
     9361 aatttctcca tactccggat aaacttttag cctaacgcag gcatcataaa ttattaaaag
     9421 cataagctta ctatccatta cattggaaac tctattgtta aacttttctt caactttttc
     9481 tggagcaaat gtagaaagat acaagtaagc agattctgaa gttgatccat agttttcttt
     9541 actctcaaac atcataaaat ttgccctgct atctactgcc caggtaactt gtctatataa
     9601 acttaaaata agttttgcag ctgaactgat tttttcaaat ttcaagttgc tatctgcata
     9661 caagttcatt atattcttta aagttttact tattctcaat ctaatcaccc caatacaatt
     9721 tatttacaat cctgaatgtt tgtgctataa tatgaatata agttcagttt gtattatatc
     9781 tcaaatcgga aaataattca agcactattg ttcgtattta aagttataaa tgcacattag
     9841 ttcaggaggt taaaattatg gcttttgcgg atagattaaa ggattttcgt gaaaaggaaa
     9901 agttaagtca agcagatttt gcaaaaatga taggaataag tacaagaact cttgtacatt
     9961 atgaagacgg agaaagatac ccaagagatg tcgaggttta caaaaaaata gctgaagtta
    10021 tggactgtga ttacaattat cttctagaag aaagtgacga gtttttaaat agagtttata
    10081 acatgggtgg caaaagagaa ttagaaaaag ctagagcatt aacagaaggt ctaagctctt
    10141 tatttgctgg tggagaaatt tctgacgaag ataaagatgc tgcttttgaa gctattactc
    10201 gtgcttattg ggaggctaaa agagaaaaca agaaatacgg tcgtaagaaa aaagattagg
    10261 tgattttatg agcatgaatc gctatatata tgataaggtt aatcgactta taaaaaaata
    10321 taagacacga gatcctatcg aattaataga agctttgaat attaacctgg tatatctacc
    10381 tgaaacaaaa atacttttag gaatgtacca ttatattcaa aggaacagat ttatttttat
    10441 cagtagtaac acaaatttta atagaaagac tatattagca catgaattag gtcacgatca
    10501 acttcacaga gattattgta tgagtggcgg agcttttcat gaccaaacag tgctaaaccc
    10561 tacaaataaa tttgaaactg aagctaatat ttttgcagct catttattaa taagtgatga
    10621 agatatccta gataatatca atgatcaagt ttgtgattat gaaatcgctg gacagttagg
    10681 tgtagatata aatttactaa atctaaaaat ttctgagtta gcaaaaatgg gcaagtttaa
    10741 taaaccagtt aatgttaata tccctcaggg agattttctt aaaaactatc gccctgaaca
    10801 tgatgattat tattaggaga ctctatggga atttttatta caggatattt tttattaatc
    10861 ctttctggaa tagttttcta ctatctcatc tctacctact ctgtaaaaat ttcagcattt
    10921 ttgattgatt ttatagttgt aggtttagga ttttatatat ccataaaaga aaagttagat
    10981 aacaaattag ctattcccct ttcaattaca gtagtaataa tttatggaat attgctagtt
    11041 ttaataaatc aaaagctacc aaggattagt aaacttttaa actatataat tgcttttata
    11101 ggatcatcag tcgccctatg gttggcactt gattttataa caaacactct aaccgctttt
    11161 aaaataatag gtcaaactta tcatcaatta ccaataacaa aaagtatgat gataaattcc
    11221 tttatacatt atgtaattgt attcttgatt agtattccag tgtttaaagg tagaatgaaa
    11281 tttatacttg gaggaaatta tgagtaaaga aatggataaa aacaaagacg aaaatcaaga
    11341 aaaaaatgag cttcaaatta gatcatctgc tgctgaatac ttgactttta ttgcagctaa
    11401 cggtgatgat caagaagcta tagaaattag atatgaagat gagaatattt ggctaactca
    11461 aaagatgatg gcagctcttt atgatgtaag tgtatcggca ataaatcagc atctaaaaaa
    11521 aatatttgac gataacgaac tggacgaaag ttcagttatt aagaaatact taataactgc
    11581 aaacgacgga aaatcatata atacaaagca ctataaccta caagcaatta ttgcagtagg
    11641 gtttaaagta aataatgaac gtgcagtaca atttagaaag tggtcaaacc aaattgtaaa
    11701 agattttaca attaaaggtt gggcgatgga tgatgaaaga ctcaagaatt ctggcacaaa
    11761 acttaccaag gattatttcg aaaaattact tgtaaaaatt cgtgaaatta gattatctga
    11821 gagaagattc tatcaaaaaa tcacagatat ttatgccacc tctttagact atgatccatc
    11881 tgcaaaagca acaaaaagat tttttgcagc tattcaaaat aaattgcact atgcaattca
    11941 tggaaaaact gcagctgaat taatcgtaga tagagccaat cacaaaacga aaaatatggg
    12001 acttacaact tgggaaggat caccagattc aaaaattcat aaatatgatg tagtcgttgc
    12061 taaaaattat ctaaacgaag aagaattaaa tcaaatgcaa agaattgttt catcttattt
    12121 ggatatggca gaaatgcaag cagaaagaca tattcctatg acaatggaag attgggaaaa
    12181 aaggttaaat ggctttttac aattatggga taaagaaatc ttaaacgacg caggaaaagt
    12241 atctgcagct ctagctaaaa aacacgcaga gactgagttt gaaaaatata gaatcataca
    12301 agataggctc tacaaaagtg attttgataa attcctagaa ctagaagatg atactaaaaa
    12361 attagaagaa taacttaata tacgcttaac gcttataact ttaatagctt taatagcaag
    12421 cacagtaagt gtacggacac ttacgaaaaa attaatggag cagcccttta cggaatgctc
    12481 cattttttta atttttacct tgttagggag aaccctaaga ccccaaaata tattttataa
    12541 aggagaataa aatggcaaat agattta
//
LOCUS       DAUCLR010000055        12298 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.055, whole genome shotgun sequence.
ACCESSION   DAUCLR010000055 DAUCLR010000000
VERSION     DAUCLR010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12298
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.055"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            62..358
                     /locus_tag="VGT87_001260"
     CDS             62..358
                     /locus_tag="VGT87_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEQ3814113.1"
                     /translation="MTKQDQLIVEKMEQTYEAFSPKLANLIEALDAFKEHYEEYATLR
                     NFYSSDEWFRLANQPWDDIPCGVLSEDLLFDMIGDHNQLLADILDLAPIMYKHM"
     gene            505..789
                     /locus_tag="VGT87_001261"
     CDS             505..789
                     /locus_tag="VGT87_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEQ3814114.1"
                     /translation="MKFKKVLVIPALALAATCFLTACGTKKDSKKEEVKEIKMSDIKD
                     DAVSKKTKVVDGEEVTEYTTKDGNVIQIPAGNEEGMESKDAGGSGAPAKN"
     gene            1213..2481
                     /locus_tag="VGT87_001262"
     CDS             1213..2481
                     /locus_tag="VGT87_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEQ3814115.1"
                     /translation="MFQLRKKMTRKQLALLSAGVLTCVVGGSYLIMNHQQQEIVSSVN
                     KVKALTIKEAMEQGKDISLTLAGEVTANNSSKVKIDSSKGEVKDVFVKKGDVVKVGQP
                     LFSYETSQRLTAQSSEFDVQTKANQLQVAKTNAALKWETYNRKVNEINTLKSRYNTAP
                     DESLLEQIRSAEDSVSQALSDAKTADSDVKTAQIELDKANATATTEKGKLEYDTVKSD
                     TAGTIVSLNTDLPNQSKSKKENETFMEIIDKSKMLVKGNISEFDRDKLKIGQKVEVID
                     RKDNSKKWTGKVTQVGNLKAEEKGQGQGQGGNDQQDNPNQAKFPYVIELDQSDKQPLI
                     GSHTYVNVLNNVPEAGKIVLKETFTMAENGKTYVWKVDKNKVKKQEIKTKPFSKGYVE
                     VTSGLTMQDKIAQPLPGMKDGMEVGSIVKP"
     gene            2468..3142
                     /locus_tag="VGT87_001263"
     CDS             2468..3142
                     /locus_tag="VGT87_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ3814116.1"
                     /translation="MLNLKDIRKSYHLGTEEFAILKGIDLEVNEGDFLAIMGPSGSGK
                     STLMNIIGCLDKPGSGSYAIEGRDVSSLSDNELADLRNQKIGFVFQNFNLMPKLTACQ
                     NVELPLTYMNVPKKERRKRALEMLKLVGLEERSEFKPMELSGGQKQRVAIARALVTNP
                     SFILGDEPTGALDTKTSVQIMDLFKQFNDNGKTIIIITHEPEVAALCKKTVILRDGNI
                     EHSDIE"
     gene            3155..4372
                     /locus_tag="VGT87_001264"
     CDS             3155..4372
                     /locus_tag="VGT87_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ3814117.1"
                     /translation="MEDIIAALSSILSHKMRSILTMLGIIIGIGAIIAIFSIIEGNTE
                     NTKRQLIGGSNNTINIVFNKKSSIDPKFPDKSNAKKPDYLPFMAEEELSKIQQVKGVK
                     NALISYGIDDKVYHLGQKSSAKISAITKNVAEVRRMTFIKGSDFSDKDFIDQKQVIYL
                     EKSLYESLFPKDDGLGKFVEVMGNPFRVIGVFESKEQSGLTSGTEKIAYIPLHQWYNI
                     NGVVDATPEITIQTYRADDLKPVAKRVSDMLNQTIPKSDYMFGVMNLKEFERQLDNLN
                     KSNFVLLAGIASISLIVGGIGVMNIMLVSVTERTREIGIKKALGARRKLILKQFLIEA
                     VILTLLGGVIGVISGMVSGLIITRSLEYPYILSLFSVVLSLAFCCIIGIVFGLLPAIK
                     ASKLDPIEALRFE"
     gene            4466..5119
                     /locus_tag="VGT87_001265"
     CDS             4466..5119
                     /locus_tag="VGT87_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ3814118.1"
                     /translation="MFKILVVEDDDTISQVICEFLKANNYDPDCVFDGQAALDKWQTT
                     SYDLIILDIMLPSLSGLEVLKTIRKTSDVPIIMLTALDDEYTQLVSFNHLISDYVTKP
                     FSPLILIKRIENVLRVSTPDEKRQIGDLLVDETEHSVYWQGTLVKLTKKEYDIIDYLA
                     KRHQKIVTRDQLMDDIWGYSELDTRVLDNHIKNLRKKMTGIPLKTITGMGYLLGERE"
     gene            5116..6504
                     /locus_tag="VGT87_001266"
     CDS             5116..6504
                     /locus_tag="VGT87_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEQ3814119.1"
                     /translation="MRLIKKTFLVINGLIIVVVTSILLVLYFAMPIYYTKVKDKEVKR
                     EFDQTSKQIKGKTVTEIRDILTKKINKDNIWYSLVDSDNQLLYPSLQLLDGVSESKDS
                     QNVNIVTTFDNSYSNVKVMSQKVTLRDGKKMTLLGQSSLQPVTDASKVLLDLYPSLLI
                     FSVTVGSIVAYLYSRTSSRRILSMSQTAKKMVNLEPNLTCTIHGKDEIAMLASDINRL
                     YASLSTSIKSLQKEYEKASDSEREKSEFLRMTSHELKTPITSVIGMIDGMLYNVGDFA
                     DRDKYLRKCRDVLEGQAQLVQSILSLSKIETLASQNQELFSLKSSLEEEMEVFLVLSE
                     LKHLKVTINLEEQFVKANKVYLLKAIKNIIDNAFHYTKSGGQVMIQLKDNQLVIKNEA
                     ETLLTQQQMKQLFQPFYRPDYSRNRKDGGTGLGLFITHQILDQHHLAYRFVVLDQRWM
                     VFTIDFPSHHDD"
     gene            6606..8234
                     /locus_tag="VGT87_001267"
     CDS             6606..8234
                     /locus_tag="VGT87_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEQ3814120.1"
                     /translation="MKKRKLLAVTLLSTILLNSAVPLVVADTSLRNNPSSTDQPTTAD
                     TDTDDESETAKKDKKSKETASQHDTQKDHKPSHNHPTPPSNDTKQTDQASSEATDKPN
                     KDKNDTKQPDSSDQSTPSPKDQSSQKESQNKDGRPTPSPDQQKDQTPDKTPEKSADKT
                     PEKGPEKATEKTPEPNRDAPKPIQPPLAAAAPVFGLWRESDKDLSKLKPSSRSSAAYV
                     RHWTGDSAYTHNLLSRRYGITAEQLDGFLNSLGIHYDKERLNGKRLLEWEKLTGLDVR
                     AIVAIAMAESSLGTQGVAKEKGANMFGYGALDFNPNNAKKYSDEVAIRHMVEDTIIAN
                     KNQTFERQDLKAKKWSLGQLDTLIDGGVYFTDTSGSGQRRADIMTKLDQWIDDHGSTP
                     EIPEHLKITSGTQFSEVPVGYKRSQPQNVLTYKSETYSFGQCTWYAYNRVKELGYQVD
                     RYMGNGGDWQRKPGFVTTHKPKVGYVVSFAPGQAGADATYGHVAVVEQIKEDGSILIS
                     ESNVMGLGTISYRTFTAEQASLLTYVVGDKLPRP"
     gene            8312..8563
                     /locus_tag="VGT87_001268"
     CDS             8312..8563
                     /locus_tag="VGT87_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814121.1"
                     /translation="MIKKVTTPSQKTKKRVRNGYLLKVGTACLLLSILSYGIGLLGQP
                     SMENTFMGIASVAMLGSVCFFIIFALNRIFDALEDNLRD"
     gene            9239..10849
                     /locus_tag="VGT87_001269"
     CDS             9239..10849
                     /locus_tag="VGT87_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ3814122.1"
                     /translation="MHVSKLFTSQQWRELKLISYLTENSNAIGVKDKELSKALNISML
                     TLQSCLTNMQFMKEVGGITYKDGYINIWYHQCCGLQEVYQKALRESPSLKLLELLFFR
                     DFSSLEELAEELFVSLSTLKRLIKKTNTYLSHTFAISIVTSPVQVSGDERQIRLFYLK
                     YFSEAYKISEWPFGDILNLKNCERLLSLLIKEVDVKVHFTLFQHLKILSGVNLIRYYK
                     GYSCSYNNKKTSHRFSQLIQHSSEIQDLSRLFYLKFGLHLDEYTIAEMFSNHLNDKLE
                     IGCAFEIINQDPTSGGRQVTNWIHLLDEMEIKLNLSITNKYEVAVTLHNASVLNEEDI
                     TANYLLFDYKKSYLNFYQKEHPRIYEAFVTSVEKLMQADNAQVSKELINQLTYCFFIT
                     WENSFLKVNQKDEKVRLLVIERSYNSVGNFLKKYIGEFFSITNFDELDCLTIDLVEIE
                     KQYDVIVTDVMVGKSEELEIFFFYKMIPEAIIDRLNEFLNVSFTDNNVMVKNLEAPSP
                     SKSHSDKEVQKPEKPEKPDNSVNQATSS"
     gene            11037..12296
                     /locus_tag="VGT87_001270"
     CDS             11037..12296
                     /locus_tag="VGT87_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407887.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEQ3814123.1"
                     /translation="MSKRNPNKHYSLRKLKTGTASVAVALTVLGTGLATGQTIKADTS
                     EQEHPNITKIKEQLLRNKLVPATQWYQAYTKSEELSRQVEKLQTLVQSKTSNYEKIKS
                     QLQELEQSKKALEDTFYSTLRDHSSLFKEQDKEIKNMRAQLEFKNKQISDLTNENAQL
                     KEAIEGYVQTIQNASREIAAKQQELAAAKSQLEAKNAEIEALKQQDASKTEEIAKLQS
                     EAATLENLLGSAKRELTDLQAKLDTATAEKAKLESQVTTLENLLGSAKRELTDLQAKL
                     DAANAEKEKLQSQAAALEKQLEATKKELADLQAKLAATNQEKEKLEAEAKALKEQLAK
                     QAEELAKLKADKASGAQKPDTKPGNKEVPTRPSQTRTNTNKAPMAQTKRQLPSTGEET
                     TNPFFTAAALTVIASAGILALKRKEES"
BASE COUNT         4448 a         2367 c         2392 g         3091 t
ORIGIN      
        1 tcatgacaat tgctagcgtt tcagtgtaaa ataaaggtaa taaccagcaa aggagcaacc
       61 catgacaaag caagatcagt taatcgttga aaaaatggaa caaacctatg aggcctttag
      121 cccaaagcta gcaaatctca tagaagccct agatgccttt aaagagcatt atgaggaata
      181 tgccacactt agaaattttt acagtagtga cgaatggttt cggcttgcaa accaaccttg
      241 ggatgatatc ccgtgcggtg tcctatctga agacctcctc tttgatatga tcggtgacca
      301 taaccaacta ctagctgata tcttagactt ggcccctatc atgtacaagc atatgtaacc
      361 attgttaacg tcacagatac tacgatgaca ggacaaaaaa agcttgaaaa tctcaaaaaa
      421 acagataaaa aacagataat cgttagaaaa acagtagatg cgtctgttaa actaaaggta
      481 tcaaaacaaa aggagcattt catcatgaaa ttcaaaaaag tattagtcat tcctgcatta
      541 gcattagcag caacatgctt tttaacagct tgtggcacaa aaaaagattc taaaaaagaa
      601 gaagttaaag aaatcaaaat gtctgacatc aaagacgacg cagttagcaa aaaaacaaaa
      661 gtggttgatg gcgaagaagt tacagaatac accacaaaag atggaaacgt gattcagatt
      721 cctgcaggaa acgaagaagg catggaatca aaagatgctg gtggatcagg cgctcctgct
      781 aaaaactaag ggttacaaca aagagcataa ggtgactcgc aaacgttacg catgacatga
      841 ggcagtctgc ctaagatgat aactcactta tcataatgcc taaatagcca aacacactaa
      901 ctcgcaaatg tacaaaatca tatacccgtg cttatgtgag cgaacggtta tatgattttt
      961 gttttccaaa aacaagaaaa gaacgctaaa atagccagtt tgtcgtgttt caagctacca
     1021 aaaaggaagt gctaaaatga aaaaaaggtc cattatacct gtcatcttac tgttggtcat
     1081 cggattaatc gttggtgtta agcgtcatca tgactagtag gtcttgctag tgaccgttca
     1141 aaagggataa cgtgggtatg gtataaccat aaaaatcgta taaaaagaaa aaatagctat
     1201 tgaggagaaa gtatgtttca gttaagaaaa aaaatgacgc gcaaacaatt agccttgttg
     1261 agtgctggag tgttgacctg tgtggttggt ggtagctact tgataatgaa ccatcaacaa
     1321 caagaaattg tctccagtgt caacaaagta aaagccttaa ccataaaaga agccatggaa
     1381 caaggaaaag atatcagctt gaccttagct ggtgaagtaa cagctaacaa cagcagcaaa
     1441 gtcaaaatcg actcaagtaa aggagaagtc aaagatgtct ttgtcaaaaa aggcgatgtt
     1501 gtcaaagtag gacaaccctt gtttagctat gaaacgtcac agcggttaac ggctcaaagt
     1561 tcagaatttg atgttcaaac caaagccaat caactccaag ttgctaaaac caatgcagca
     1621 ttgaagtggg aaacctacaa tcgcaaggtc aatgaaatca acaccctaaa atctcgctac
     1681 aacactgcac cagatgagag cttactagag caaattcgca gcgcagaaga cagtgtctct
     1741 caagcactaa gcgatgccaa aacagcagat agcgatgtca aaaccgctca aatagaactc
     1801 gataaagcta atgctactgc cacaacggaa aaaggtaaac tagagtatga caccgttaag
     1861 tcagacaccg caggaaccat tgttagccta aatactgatt tgccaaatca atcaaaatcc
     1921 aaaaaagaaa atgaaacttt tatggaaatt atcgacaaat caaaaatgtt agtcaaaggt
     1981 aacatcagtg aatttgaccg tgacaagtta aaaatcggtc aaaaagtcga agtgattgac
     2041 cgcaaagaca actctaaaaa atggactgga aaagtaaccc aagttggcaa cctcaaagca
     2101 gaggaaaaag gccaaggtca aggccaaggt ggcaatgacc aacaagacaa tccaaaccaa
     2161 gcaaaattcc cttatgttat tgaacttgac caatcagaca agcagccact cattggctca
     2221 cacacttatg ttaatgtgct caacaatgtt ccagaagctg gcaagatcgt attgaaagaa
     2281 acctttacaa tggcagaaaa tggaaaaacc tatgtgtgga aagttgataa aaacaaggtc
     2341 aaaaaacaag aaatcaagac taagccattc tcaaaaggtt atgttgaggt gacaagtggc
     2401 ttgactatgc aagataagat tgctcagccg cttcctggca tgaaagacgg tatggaggta
     2461 ggaagtattg ttaaacctta aagatattcg aaaaagctat catcttggga ctgaagaatt
     2521 tgcgatttta aaaggaatcg atttagaagt taacgagggt gactttttag ccatcatggg
     2581 accatcaggt tcgggaaagt caacattgat gaatatcatt gggtgtttag ataagcctgg
     2641 ctctggctca tatgccattg aaggcagaga cgtgtcatcc ttatctgata atgaacttgc
     2701 tgatttgcgc aatcaaaaaa tcggctttgt ttttcaaaac tttaacctga tgcccaagct
     2761 aacagcttgt caaaatgtcg aattgccctt gacttatatg aatgttccta aaaaagagcg
     2821 tcgcaaacga gccctagaga tgttaaagct agtaggatta gaagaacgta gtgaatttaa
     2881 accgatggag ctatctggtg ggcaaaaaca gcgtgtagcg attgcaagag ctttagtcac
     2941 taacccgagt tttatccttg gtgatgagcc aacaggtgca ctagacacaa aaaccagcgt
     3001 ccaaatcatg gacctattta aacaattcaa tgataacggc aaaacgatta ttatcatcac
     3061 acacgagcct gaagtagctg ccttatgcaa aaagacggtg atcctaagag atggtaatat
     3121 agaacattcc gatatagagt aaagggggaa aataatggaa gatattattg cagctttaag
     3181 ctctatatta tcacacaaga tgcggtcaat tttgacaatg ctagggatta ttatcggtat
     3241 tggtgccatt atcgcaatct tttcaatcat cgaaggtaat accgaaaata ccaaaagaca
     3301 gttgattggt ggcagcaata acactattaa cattgtgttt aacaaaaaaa gcagtattga
     3361 ccctaaattt ccagacaagt caaatgctaa aaaaccagac tatcttcctt ttatggctga
     3421 agaagagctt agcaaaatac agcaagtcaa aggtgtcaaa aacgccctga tctcatatgg
     3481 tattgatgac aaagtctacc atctaggcca aaaatcatct gcaaagattt ctgccatcac
     3541 aaaaaacgta gctgaagtga ggcgcatgac atttattaaa ggcagtgact tttcagacaa
     3601 agactttatt gaccaaaaac aggtgattta ccttgaaaaa agcttgtatg agtcactatt
     3661 tccaaaagac gatggccttg gaaaatttgt cgaagtgatg ggaaatccct tcagggtcat
     3721 tggggtgttt gaatcaaaag aacaaagtgg cctcacaagt gggactgaga aaattgctta
     3781 tatcccgcta catcagtggt ataatattaa tggtgtagtg gatgcgacac ctgagatcac
     3841 gatccagacc tatcgcgcag atgacctcaa gcctgtagca aagcgtgtca gtgatatgct
     3901 taatcaaacg atccctaaat cagattatat gtttggtgtc atgaacctta aagaatttga
     3961 acgccaacta gacaacctaa acaaatctaa ttttgtgtta ttagctggta ttgctagtat
     4021 ctctcttatt gtaggtggta ttggtgtgat gaacattatg ctagtatcag ttaccgaaag
     4081 aacgagagaa attgggatca aaaaagcgct tggcgctaga cgtaagctta ttttaaaaca
     4141 atttttaatt gaagcggtca ttctaacctt gctaggtggt gtgatagggg ttatttcagg
     4201 gatggtatca ggattgatca tcacaagatc actcgagtat ccctatatcc tatccttgtt
     4261 ctcagttgtc ttgagtttag cgttttgttg tattataggg attgtctttg gactattacc
     4321 agccattaaa gcgtctaagc tagacccaat tgaagcattg cgatttgaat aatcaaccaa
     4381 acaagcataa aagtcaaaaa ggatgaccct ttgatgtcat ccttagctgc ttgatacact
     4441 aaacgccaat ctagaaaggg aagttatgtt taaaatatta gtagttgaag atgatgatac
     4501 catcagtcaa gtgatttgtg agtttttaaa agcgaataac tatgatccag attgtgtatt
     4561 tgatggccaa gctgccttag ataagtggca gacaacgtct tatgatctga ttattttaga
     4621 catcatgctc ccttccttaa gtggcttaga agtgctaaaa acaattcgaa agacatcaga
     4681 tgtccctatt attatgctaa cggcattaga tgacgagtac acacagcttg taagcttcaa
     4741 ccatttgatt agtgattatg tgaccaaacc attttcacca ctcattttaa tcaaacgtat
     4801 cgaaaatgta ttacgcgtct ctactccaga tgaaaagcgc caaatcggag acttacttgt
     4861 tgatgagacc gaacatagcg tctattggca aggaacattg gtcaaactaa ccaaaaaaga
     4921 atatgacatt attgattatt tagcaaaacg tcaccaaaag attgtcacca gagaccaatt
     4981 gatggatgac atatggggct attctgaact ggacacgcgt gtccttgaca accacatcaa
     5041 gaaccttcgt aaaaaaatga cgggtattcc cctcaagaca attacaggca tgggctattt
     5101 attgggagag cgtgagtgag actaatcaaa aaaacctttt tggtcatcaa tggcttaatt
     5161 attgtagtgg ttacctctat tttgttagtg ctttattttg caatgccaat ttattacacc
     5221 aaagtcaaag acaaagaagt taagcgtgaa tttgaccaaa caagcaagca aatcaaaggc
     5281 aaaacggtaa ccgagattag agatatccta acaaaaaaaa tcaacaaaga taatatttgg
     5341 tacagcttag tagacagtga taaccaactg ctctatcctt ccttgcagct cctagacggc
     5401 gtcagtgaaa gcaaagacag tcaaaatgtt aatatcgtca ccacctttga caactcttat
     5461 tctaatgtta aggtgatgag tcaaaaagta accctacgcg atggaaaaaa aatgacattg
     5521 cttgggcaat cttcacttca acccgtaaca gatgctagca aagttctcct tgacctctac
     5581 ccgtcactat tgattttttc agtgaccgta gggagtattg tcgcctactt atatagtagg
     5641 acttctagcc ggcgtatcct tagcatgtca caaaccgcca aaaaaatggt caacctagaa
     5701 ccaaacttaa cgtgtaccat tcacggcaaa gatgaaatag caatgttggc tagtgatatt
     5761 aaccgtttgt atgcgagtct ttcaacgagc atcaaatcct tgcaaaaaga gtatgaaaaa
     5821 gcttctgatt cagaaagaga gaaatctgag tttttacgca tgacatcgca cgagctaaag
     5881 acaccaatca ctagtgttat cggaatgatt gatggaatgc tctataatgt tggtgacttt
     5941 gcagatcgtg acaaatattt gcgaaaatgc cgagacgtcc ttgaagggca agcccaactg
     6001 gttcaatcca ttttatcttt atctaagatt gaaaccctag cttctcaaaa tcaagaactg
     6061 ttttcactaa aaagcagcct agaagaagaa atggaagtct ttctcgtttt atcagaacta
     6121 aaacacctca aagtgactat caatctcgaa gaacaattcg tcaaagccaa taaagtatac
     6181 ctactaaagg cgattaaaaa tattattgac aatgcctttc actataccaa atcaggcggc
     6241 caagtgatga ttcaactaaa agacaaccaa ctagtgatta aaaatgaagc agagacatta
     6301 ttgacacaac agcagatgaa acagttattc caaccgtttt atcgaccaga ttatagtcgt
     6361 aacaggaaag acggtggtac aggattgggg ctgtttatta cccaccagat tcttgatcag
     6421 catcatctgg cttatcgctt tgttgttctt gatcaaagat ggatggtatt tacgattgat
     6481 tttccatccc atcatgacga ttgatccaca ctagtaggac gacaaaagtc atcaatcatt
     6541 aaccaagcct tgacctttac ccatcattgt ttaggcaagt ataaaaaaga ggagagaaat
     6601 catatatgaa gaaaaggaaa ttgttagcag taacactatt aagtaccata ctcttaaaca
     6661 gtgcagtgcc attagttgtt gctgacacct ccttgcgtaa taacccatca tccactgatc
     6721 agcctactac agcagatact gatacggatg acgagagtga aacagcaaaa aaagacaaaa
     6781 aaagcaagga aacagcgtcg cagcacgaca cccaaaaaga ccataagcca tcacacaatc
     6841 acccaacccc cccttcaaat gatactaagc agaccgatca ggcatcatct gaagctactg
     6901 acaaaccaaa taaagacaaa aacgacacca agcaaccaga cagcagtgat caatccaccc
     6961 catctcccaa agaccagtcg tctcaaaaag agtcacaaaa caaagatggc cgacctaccc
     7021 catcacctga tcagcaaaaa gatcagacac ctgataaaac accagaaaaa tcagctgata
     7081 aaacccctga aaaaggacca gaaaaagcaa ctgaaaaaac tccagagcca aatcgtgacg
     7141 ctccaaaacc catccaacct cctttagcag cagcagctcc tgtctttgga ctttggagag
     7201 aaagtgacaa agacctgagc aagctaaaac caagcagccg ctcatcagcc gcttacgtga
     7261 gacactggac aggtgactct gcctacactc acaacctgtt gtcacgccgt tatgggatta
     7321 ctgctgaaca gctagatggt tttttgaaca gtctaggtat tcactatgat aaagaacgct
     7381 taaacggaaa gcgtttatta gaatgggaaa aactaacagg actagacgtt cgagctatcg
     7441 tagctattgc aatggcagaa agctcactag gtactcaggg agttgctaaa gaaaaagggg
     7501 ccaatatgtt tggttatggt gcccttgact tcaacccaaa caatgccaaa aaatacagcg
     7561 atgaggttgc tattcgtcac atggtagaag acaccatcat tgccaacaaa aaccaaacct
     7621 ttgaaagaca agacctcaaa gcaaaaaaat ggtcactagg ccagttggat accttgattg
     7681 atggtggggt ttactttaca gatacaagtg gcagtgggca aagacgagca gatatcatga
     7741 ccaaactaga ccaatggata gatgatcatg gaagcacacc tgagattcca gaacatctca
     7801 agataacttc cgggacacaa tttagcgaag tgcccgtagg ttataaaaga agtcagccac
     7861 aaaacgtttt gacctacaag tcagagacct acagctttgg ccaatgcact tggtacgcct
     7921 ataatcgtgt caaagagcta ggttatcaag tcgacaggta catgggtaac ggtggcgact
     7981 ggcagcgcaa gccaggcttt gtgaccaccc ataaacctaa agtgggctat gtcgtctcat
     8041 ttgcaccagg ccaagcagga gcagatgcaa cctatggtca cgtcgctgtc gtagagcaaa
     8101 tcaaagaaga tggttccatc ttgatttcag agtcaaatgt tatgggatta ggcaccattt
     8161 cctatcggac gttcacagct gagcaggcta gtttgttgac ctatgtcgta ggggacaaac
     8221 tcccaagacc ataaccccac aaccaagaag ctacctgcct gttgaccaat ctcaaaaaga
     8281 gatagcgtca acttagaatg aggttcatca gatgataaag aaagtgacca ccccgagtca
     8341 aaaaacaaaa aaacgtgttc gtaatggata cctcctaaaa gtaggtacag cctgtttact
     8401 cttaagtata ctcagttatg gcattggtct tttgggccag cctagcatgg aaaatacgtt
     8461 tatgggaata gcgagtgttg ccatgttagg aagtgtctgt ttctttatta tctttgctct
     8521 aaaccgtatc tttgacgcac tagaggacaa tttgcgagat tagagtaata ggtcaaataa
     8581 tcttccaata atttagagca caagctaagt tattggaagt ttttttgtct tttgagagcc
     8641 atgatgccat caaaaagcca aacactcctt atgatgtgag agtcacatga ctaaaattaa
     8701 aagcaaaata gaccccaaag ccctgttttc tcaaaacagt caacgaaaaa agacaagtta
     8761 accagttcat aaaaaacatc gtagaaacat tttcaagagc taatgttggt gtaaattgac
     8821 tgaagtatga tagaattttt aatgaatgtg acaataatgt cacagataag aaccgaaaac
     8881 agcaaaaata attaaattga atataaaaaa gttaattaag tgagtgataa taaagaaatg
     8941 aagttcaaaa aatataaata aaataaaaca ccagaactgc taaaagcagt gaatgacata
     9001 aatgtcgcta aaaaaataga tagaagagtc taaaatttca gttttaggtc acgttttatg
     9061 gatagaaatc aggatgatga ccttaagttg tgatttgcgc atcattgacc gctttactcg
     9121 ttttaatgaa agaatttgaa agatatttta tggtatactt ttccttaata tggttcatac
     9181 ggacttgata aatctattta caaaaataat aaaaaattaa gataaggaca tgaagttaat
     9241 gcatgtaagt aaattgttta ctagccaaca atggagagaa ttgaaactga tctcatattt
     9301 aacagaaaac tccaatgcta tcggagttaa agataaggag cttagcaagg ctctcaatat
     9361 ttccatgtta acattacagt catgcctcac taatatgcag tttatgaaag aagtaggtgg
     9421 aattacctac aaagacggct atattaacat ttggtatcac cagtgttgtg ggctacaaga
     9481 ggtctatcaa aaagctcttc gagaatcgcc ttctttgaaa ttactggagc ttttgttttt
     9541 cagagacttt agttccttag aagagttagc tgaagagctg tttgtcagtt tatctaccct
     9601 caaacgcctc atcaaaaaaa ccaacactta cttgtctcat acctttgcta ttagtatcgt
     9661 gacaagtcca gtgcaggtat caggagatga gaggcaaatc cgcctgtttt acctcaagta
     9721 tttttcagaa gcctataaaa tctcagaatg gccatttggg gacattttaa acctgaaaaa
     9781 ctgtgaacgc ctattgagct tattgatcaa agaagtagat gtcaaagttc attttacact
     9841 atttcagcac ttaaaaatct taagtggtgt caacttgatc cgttactaca aggggtattc
     9901 ttgcagttat aacaataaaa aaaccagtca ccgtttttct caacttatcc aacattcctc
     9961 agaaatccag gatctttctc gcttgttcta cctcaaattt gggttacatc tcgacgagta
    10021 taccatagct gaaatgtttt ctaaccatct taacgacaag ctagaaattg gctgtgcttt
    10081 tgagattatc aatcaagacc caacatcagg aggcagacaa gtaaccaact ggatccatct
    10141 attagatgag atggaaatca agctcaatct cagcatcacc aataaatacg aagttgctgt
    10201 aaccctccat aacgcttcgg tgttaaacga agaagacatt acagccaatt atttgctgtt
    10261 tgattataaa aagagttacc tgaactttta ccaaaaagaa caccctcgta tctatgaggc
    10321 ctttgtgaca agtgtcgaga agctgatgca ggcagataat gctcaagtta gcaaagaatt
    10381 gatcaatcag ctcacttatt gtttctttat tacctgggaa aatagtttct taaaagtaaa
    10441 tcaaaaagat gaaaaagtac gacttctagt gatagagaga agttacaata gtgtcggtaa
    10501 ttttttgaaa aagtacatcg gtgagttttt cagcattact aattttgatg agttagattg
    10561 tttgacaatt gatctagtag agattgaaaa acagtacgat gttatcgtga cagatgttat
    10621 ggtgggtaaa agcgaagagt tagagatttt ctttttctac aagatgatcc cagaagctat
    10681 tattgataga ttaaacgaat ttttgaacgt tagtttcaca gataacaacg ttatggtcaa
    10741 aaacctcgaa gccccttccc cttcaaaatc tcatagtgac aaagaggtac aaaagccaga
    10801 aaagccagaa aagccagaca attcagttaa tcaagcaaca tcatcatagg atttcagacg
    10861 tcatggtaag aaaaaaaaga atcatttaca gcccatagag gtaaggtcaa aagctgaaag
    10921 cagctcaaaa aaactgacct ttaccttttg gcttttttta tttagaataa ttttattgga
    10981 gagatgctta ataatttaag cacaattctt agaaattgag aaataaggag taaacaatgt
    11041 ctaaaagaaa tccaaacaaa cactactcac tgagaaagtt aaaaacaggt actgcatcag
    11101 tagcagtagc tttgacagtt ttggggactg ggttagcaac tggacaaaca ataaaagctg
    11161 ataccagtga acaagaacac ccgaacatta caaagatcaa agaacagctt ttacgcaata
    11221 agttggtccc tgctacacaa tggtaccaag cctatactaa aagcgaggaa cttagtcgtc
    11281 aggtcgaaaa acttcagacc ttggtccaat caaagacctc aaattatgaa aagataaagt
    11341 cacagttaca agagttggag caatctaaaa aggctttgga ggacactttt tacagtacac
    11401 ttagagatca ctcaagtctt ttcaaagaac aagataaaga aatcaaaaat atgagggccc
    11461 agcttgagtt taaaaacaag caaatctcag atttaaccaa cgaaaacgca cagttaaaag
    11521 aagccattga aggttatgtg caaactatcc aaaacgctag tcgtgaaatc gcagcaaaac
    11581 aacaagaact tgcagctgca aaaagccagt tagaggcaaa aaatgctgag attgaggcat
    11641 tgaaacaaca agatgcctct aagactgaag aaattgctaa attgcaatca gaagcagcaa
    11701 ctttagaaaa ccttctaggt tcagccaagc gtgagttgac tgacttgcaa gctaagctag
    11761 atacagcaac tgctgaaaaa gcaaaactag aatcacaagt aacaacctta gaaaaccttc
    11821 taggttcagc taagcgtgaa ttgactgatc tgcaagctaa gctagatgca gctaacgctg
    11881 aaaaagaaaa gctccaatca caagcagcag ccctagaaaa acaactagaa gcaactaaaa
    11941 aagagttagc tgatttacag gctaaattag cagcaaccaa ccaagaaaaa gaaaagttag
    12001 aagctgaagc aaaagctctt aaagagcaat tagcaaaaca agctgaagag cttgctaagc
    12061 taaaagcaga taaagcttca ggggctcaaa aaccagatac taaacctggc aataaagagg
    12121 ttccaacaag accatcacaa acaagaacaa acactaataa agctcctatg gcgcaaacaa
    12181 agagacaatt accgtcaaca ggcgaagaaa caaccaaccc attcttcact gcagcagcat
    12241 tgacagtgat cgcatctgca ggcatacttg ccctaaaacg caaagaagaa agctaagc
//
LOCUS       DAUCLR010000056        12016 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.056, whole genome shotgun sequence.
ACCESSION   DAUCLR010000056 DAUCLR010000000
VERSION     DAUCLR010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12016
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.056"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            62..135
                     /locus_tag="VGT87_001271"
     tRNA            62..135
                     /locus_tag="VGT87_001271"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:96..98,aa:Asn,seq:gtt)
     gene            complement(632..3208)
                     /locus_tag="VGT87_001272"
     CDS             complement(632..3208)
                     /locus_tag="VGT87_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEQ3814124.1"
                     /translation="MKNNNKWIIAGLASFLFPLSIIFIILLSMGIYYNSDKTILASDA
                     FHQYVIFAQNFRNIMHGSDSFFYTFTSGLGINFYALMCYYLGSFFSPLLFFFNLASMP
                     DAIYLFTLIKFGVIGLAACYSFHRLYPKISAFLMISISVFYSLMSFLTSQMELNSWLD
                     VFILLPLVVLGLNKLITENKTRTYYLSISLLFIQNYYFGYMVALFCILYALVCLLSLN
                     DFNKMFIAFVRFTAVSICAALTSALVILPTYLDLSTYGENLSPVKQLVTNNAWFLDIP
                     AKLSIGVYDTTKFNALPMIYVGLFPLMLSVIYFTLESIPLKIKLANACLLTFIIISFY
                     LQPLDLFWQGMHSPNMFLHRYAWSFSIVILLLACETLSRLKEVTQIKAGFAFIFLIIL
                     TSLPYSFSQQYNFLPLTLFLLSVFLLLGYTISLFSFRNSQIPSTFISAFILIFSLLES
                     GLNTYYQLQGINKEWGFSSRQIYNSQLKDINNLVNSVSKNSQPFFRMERLLPQTGNDS
                     MKFNYYGISQFSSVRNRLSSSLLDRLGFQSKGTNLNLRYQNNTIIMDSLLGIKYNLSE
                     GPLNKFGFTKLKTSGNTTLYQNHYSSPLAILTRNVYKDVNLNINTLDNQTKLLNQLSG
                     KSLTYFNLQPAQLISGANQFNGQISAQASDYQNSVTLNYQINIPKHSQLYISIPNIIF
                     SNPDAKEVRIQTDNHNFIYTTDNAYSFFDLGYFADAKVATFSFVFPKNKQISFKEPHF
                     YSLSIESYLEAMNSIKQKNVHTYAKSNTVITDYNSKTKGSLIFTLPYDKGWSAQKDGK
                     NLPVKKAQGGFLSVTIPKGKGRVILTFIPNGFKLGLSLSCVGIIAYMLLYKYIDIKSK
                     LL"
     gene            complement(3291..4910)
                     /locus_tag="VGT87_001273"
     CDS             complement(3291..4910)
                     /locus_tag="VGT87_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEQ3814125.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTAGNTYGLIGANGAGKSTFL
                     KILAGDIEPSTGHISLGPDERLSVLRQNHFDYEEERAIDVVIMGNEQLYNIMKEKDAI
                     YMKADFSEEDGVRAAELEGIFAELGGWEAESEASQLLQNLNIPEDLHYQNMSELANGD
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSISWLEDFLIDFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLFVGNYDFWKQSSELAARLQADRNAKAEEKIKELQEFVARFSANA
                     SKSKQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREMGNDFLTVENLSVTIDGEKI
                     IDNISFILRPGDKAAIIGQNDIQTTALMRALADDIDYEGTIKWGVTTSRSYLPKDNSK
                     DFATEESILEWLRQFASKGEDDDTFLRGFLGRMLFSGDEVKKSVNVLSGGEKVRVMLS
                     KLMLLKSNVLILDDPTNHLDLESISSLNDGIKDFKESVIFASHDHEFIQTIANHIVVI
                     SKNGVIDRIDETYDEFLDNPEVQARVAELWK"
     gene            complement(4989..5861)
                     /locus_tag="VGT87_001274"
     CDS             complement(4989..5861)
                     /locus_tag="VGT87_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEQ3814126.1"
                     /translation="MKKRGIDLLLVTIGSFITAIGFNTMFVDNHIASGGMVGIAVVIK
                     ALFGISPSLFLMASNIPLLLMCYFFLGKQNFIKTLYGSWIYPIAIRSTNSLPTLTHNQ
                     LLAAIFGGIICGIGLGMVFWGNSSTGGTGILTQILHKYSPLSLGVAMTIVDGISVLMG
                     FIALSADDVMYSTIGLFVIGYVISVMENGFDSSKNVMIISKDYQAIREYITTVMDRGV
                     TKLPIRGGYTTSDKIMLMAIVSSHELPTLQEKILEIDDTAFIVVMPAAQVMGRGFSLT
                     KQYKREDKDVLLPM"
     misc_binding    5984..6225
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6280..7302
                     /gene="trpS"
                     /locus_tag="VGT87_001275"
     CDS             6280..7302
                     /gene="trpS"
                     /locus_tag="VGT87_001275"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEQ3814127.1"
                     /translation="MTKPIILTGDRPTGKLHLGHYVGSLKNRVFLQNENKYKMFVFLA
                     DQQALTDHAKESELIQESIGNVALDYLSVGLDPKQSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEIAQKGFGESIPSGFLVYPVSQAADITAFKANLVPVGNDQKPMIE
                     QTREIVRSFNHTYHTDCLVEPEGIYPENEKAGRLPGLDGNAKMSKSLGNGIYLSDDAD
                     TVRKKVMSMYTDPNHIKIEDPGQIEGNMVFHYLDIFARKEDQADIEAMKEHYQRGGLG
                     DVKTKRYLLDILERELAPIRERRLEYAKDMGEVFRMLQEGSQKARTVAAKTLSEVKSA
                     MGINYF"
     gene            7610..9091
                     /gene="guaB"
                     /locus_tag="VGT87_001276"
     CDS             7610..9091
                     /gene="guaB"
                     /locus_tag="VGT87_001276"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEQ3814128.1"
                     /translation="MSNWDTKFLKKGYTFDDVLLIPAESHVLPNEVDLKTKLADNLTL
                     NIPIITAAMDTVTGSKMAIAIARAGGLGVIHKNMSITEQAEEVRKVKRSENGVIIDPF
                     FLTPEHKVSEAEELMQRYRISGVPIVETLANRKLVGIITNRDMRFISDYNAPISEHMT
                     SEHLVTAAVGTDLETAERILHEHRIEKLPLVDNSGRLSGLITIKDIEKVIEFPHAAKD
                     EFGRLLVAAAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPNR
                     TLIAGNIATAEGARALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIYQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAASDIVFQMLGGIRSGMGY
                     VGAGDIQELHENAQFVEMSGAGLIESHPHDVQITNEAPNYSVH"
     gene            9294..10157
                     /locus_tag="VGT87_001277"
     CDS             9294..10157
                     /locus_tag="VGT87_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HEQ3814129.1"
                     /translation="MEGIFYALIPMFTWGSIGFVSNKIGGKPSQQTLGMTFGALLFSL
                     AVWLIVRPEMTLQLWIFGILGGFIWSIGQTGQFHAMQYMGVSVANPLSSGSQLVLGSL
                     IGVLVFHEWTKPMQFVVGSLALLLLIVGFYFSSKQDDANAQVNHLHNFSKGFRALTYS
                     TIGYVMYAVLFNNIMKFDVLSVILPMAFGMVLGAITFMSFKISIDQYVIKNSVVGLLW
                     GIGNIFMLLAASKAGLAIAFSFSQLGAIISIVGGILFLGETKTKKEMRWVVTGIICFI
                     VGAILLGVVKS"
     gene            complement(10213..11319)
                     /gene="recF"
                     /locus_tag="VGT87_001278"
     CDS             complement(10213..11319)
                     /gene="recF"
                     /locus_tag="VGT87_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEQ3814130.1"
                     /translation="MWIKELELKHYRNYDHLLASFSSGLNVFIGNNAQGKTNFLEAIY
                     FLSLTRSHRTRADKELIHFDHSTVSLTGKIQRISGTVDLEINLSDKGRVTKINALKQA
                     KLSDYIGTMMVVLFAPEDLQLVKGAPSLRRKFIDIDLGQIKPVYLSELSHYNHVLKQR
                     NSYLKSAQQIDAAFLAVLDEQLASYGARVMEHRIDFINALEKEANTHHQAISNGLESL
                     SLSYQSSVVFDKKTNIYQQFLHQLEKNHQKDFFRKNTSVGPHRDDLAFYINGMNANFA
                     SQGQHRSLILSLKMAEVSLMKALTGDNPILLLDDVMSELDNTRQTKLLETVIKENVQT
                     FITTTSLDHLSQLPEGIRIFHVTKGTVQIDSDIH"
     gene            complement(11321..11734)
                     /gene="yaaA"
                     /locus_tag="VGT87_001279"
     CDS             complement(11321..11734)
                     /gene="yaaA"
                     /locus_tag="VGT87_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEQ3814131.1"
                     /translation="MIYKLFTEFITLQALLKELGIIQSGGAIKRFLAETTVLFNGEDE
                     KRRGKKIRVGDKISLPDQDLIITIVEPSQEEKEQFAEEIAEKTRVAALVKQMNQANKK
                     TSSKHKNRQSTTKKSLRATKKTKGKPTAPVRFPGI"
BASE COUNT         3915 a         1937 c         2247 g         3917 t
ORIGIN      
        1 aacacgggtt cgaatcccgt acggactatc gcaattgtca ttttggcaat ttttttcttt
       61 ttccggcata gctcagttgg tagtagcgca tgactgttaa tcatgatgtc gtaggttcga
      121 gtcctactgc cggagtgata agatatttcc taagataatc ttaggatttt tttatttagt
      181 aaaacagata ctcaggtcaa atactcaatt tactaattat taaaccacta aatacagcat
      241 tgtgaactaa caggattgta ttttgcatta aaatagaagt tctgttcttt aattgtatag
      301 atgataacat taagtttaat tacaaacatt aactgaagct taatttttta cattttgaaa
      361 gcatgtctcc catttaatac tgatgaatat aagattattg taaggagtca aaagattgaa
      421 atcgtatcag cgatgattat tgtcatttca atctttgaaa gagagtagtt taacattaac
      481 cctcacgaaa aatctgacct ttatattatt tgagtttatt agtaaaatcg ataaaagcat
      541 ctcaagtatt agaatatttg tctagctctt gagatgcttt tagtatattg actcatttgc
      601 caaattgata aaaaaatctg atttttttgg actaaagtaa tttagacttt atatctatgt
      661 acttatacaa aagcatataa gcgataattc ctacgcaaga tagagataac cctaatttaa
      721 aaccattagg aataaaggta aggataacac gtccctttcc tttaggaata gtcactgata
      781 gaaatcctcc ttgtgctttt ttgactggaa gatttttccc atctttttgt gctgaccaac
      841 ctttatcgta aggaagtgta aaaataagag aacctttcgt ttttgaatta taatcagtga
      901 ttaccgtatt acttttagcg taagtatgaa catttttttg tttaatacta ttcattgctt
      961 caaggtaaga ttcaatagac aaactataaa aatgaggttc cttaaaacta atttgtttat
     1021 tttttggaaa aacaaacgaa aatgtagcaa ctttggcatc ggcgaaatat cctaaatcaa
     1081 aaaaagagta agcgttatct gtagtatata tgaaattatg attatctgtc tgaatacgca
     1141 cctctttagc atcaggattt gaaaatataa tattgggtat gctaatatag agttgactat
     1201 gtttagggat gttaatttga taattaaggg taacggagtt ttgataatca gaagcttgtg
     1261 cagatatttg tccgttaaat tgattagcac cagaaataag ttgagctggc tgtaagttaa
     1321 aataggttaa agatttccca cttagttggt taagtaattt ggtttggtta tcaagggtat
     1381 tgatatttag gttgacatct ttgtaaacat tacgtgttaa tatagctaaa gggctactat
     1441 agtgattttg ataaagagta gtattcccgc tagtttttag ttttgtaaat ccaaatttat
     1501 ttagaggtcc ttcgctaaga ttatatttta taccaagtag actgtccata ataatagtat
     1561 tgttttggta tctaaggttt aaatttgtgc ctttagactg aaatcccaat cgatccaata
     1621 aagaactaga tagtctattt cttacagagg aaaattgtga aatgccgtaa taattaaatt
     1681 tcatgctatc gttccctgtt tggggaagta gtctttccat tctaaaaaaa ggttgactat
     1741 tttttgacac agagttgaca aggttgttaa tatcctttaa ttgactatta tatatctgtc
     1801 gtgatgagaa tccccactcc ttattaattc cttgaagctg gtagtaggtg tttaaccctg
     1861 attcaagaag gctaaagata agtatgaaag cagaaataaa agtagatggg atttgagaat
     1921 ttctaaacga aaatagtgaa atagtataac ctaataataa aaaaacacta agtaaaaaaa
     1981 gagttaaagg tagaaaatta tattgttgag aaaagctata aggaagagat gtcagtataa
     2041 tgaggaaaat aaaagcaaaa cctgctttta tttgagtcac ttcttttagt cgagagagag
     2101 tctcacatgc gagtaatagg ataactatgg aaaaagacca agcgtagcga tgcaaaaaca
     2161 tatttggtga gtgcatcccc tgccaaaaaa gatcaagtgg ctgtaggtaa aaacttatta
     2221 taataaaagt taacaagcag gcattggcta attttatttt taaagggata ctttctaaag
     2281 taaaataaat aacactaagc attaggggaa ataatcctac gtaaatcata ggcagagcat
     2341 taaacttggt agtatcgtac actcctattg agagcttagc aggtatatcc aaaaaccaag
     2401 cattgttcgt aactaactgt tttaccgggg atagattctc tccataagtt gacaaatcta
     2461 gataggtagg aagtattact agagcacttg ttaaagcagc acatattgac acagctgtaa
     2521 acctaacaaa agcgataaac attttgttaa aatcattgag acttaaaaga caaactaagg
     2581 cgtaaagaat acaaaaaaga gcaaccatgt agccaaagta gtaattttga atgaataata
     2641 atgatatcga aagataataa gttctggttt tattttctgt gataagttta tttaatccaa
     2701 gtacaacaag tggaagaaga atgaaaacat ctaaccaaga atttagttcc atttgacttg
     2761 tcaagaagct cattaagcta taaaaaactg agatggaaat catcaagaaa gcactgattt
     2821 ttggatataa tctatgaaaa gaatagcatg cagctaatcc tattacccca aattttatca
     2881 aggtaaacaa atagatagca tctggcatag aggctaaatt aaaaaagaaa agtaatggag
     2941 aaaagaaact gccaagataa taacacatta aagcataaaa atttatccct agtccgcttg
     3001 taaaggtata aaaaaaacta tcagaaccgt gcatgatgtt acgaaagttc tgcgcaaaaa
     3061 taacatactg atgaaaagca tcactagcta gaattgtttt atcactatta taataaatgc
     3121 ccatcgatag aaggatgata aatataatac taagagggaa caaaaaacta gcaagtccag
     3181 caattatcca tttattatta tttttcataa attatatggt ctttcgttta caaattttaa
     3241 aaaagaggct aggaagagat cttcttagcc acctcattta tcataaggtt ttatttccaa
     3301 agttcagcaa ctcgggcttg gacttctgga ttgtcaagga attcgtcata agtttcatct
     3361 atacggtcga taacaccatt ttttgaaata acaacgatgt ggttagcaat cgtttgaata
     3421 aattcatggt catgactggc aaagataaca gattctttga agtctttgat cccatcatta
     3481 agacttgaaa tagattccaa atccaagtga tttgttggat catcaagaat aagaacgttt
     3541 gatttcaata gcatcaactt agaaagcatg acacgcactt tttcgccacc ggaaagaacg
     3601 ttaacagatt ttttaacttc gtctccagag aaaagcatcc gtcctaggaa accacgtaaa
     3661 aaagtatcgt catcttcacc ttttgaggca aattgacgta accattcaag aatagattct
     3721 tccgtggcaa aatcttttga attgtcttta ggcaagtaag aacgacttgt tgtcactccc
     3781 catttaatgg ttccttcgta gtcaatatca tctgctaaag cacgcataag agcggttgtt
     3841 tgaatatcat tttgaccaat aatagcagct ttgtcacctg gacgtaaaat gaaactgata
     3901 ttatcaatga ttttctctcc atcgatcgtt acagaaagat tttcaacagt gaggaagtca
     3961 ttccccattt cacgctctgc tttaaaatta ataaatgggt atttacggct tgagggaaca
     4021 atttcttcta attcaatctt atcaagcatt tttttacgag aggttgcttg ttttgattta
     4081 gatgcgtttg ctgagaaacg ggcaacgaat tcttgtaatt ctttgatttt ttcttcggct
     4141 ttagcattgc ggtcagcttg taaacgagcg gctagctcag aagattgttt ccagaaatcg
     4201 tagttaccca caaagagttt gattttacca aagtcaaggt cggccatgtg agtacatact
     4261 ttattcaaga agtgacggtc atgggaaaca acgatgactg tattttcaaa atcaatcaag
     4321 aaatcttcca accatgaaat agattgaata tcaagaccat tggttggctc gtcaagtaat
     4381 aaaacgtcag gcttaccaaa gagcgcttta gcaagaagga ctttcacttt atctccatta
     4441 gccagttcac tcatattttg gtagtgaagg tcttctggaa tatttagatt ttggagaagt
     4501 tgagaagctt cactttctgc ctcccagcca cctaattcag caaagatacc ttctagctca
     4561 gcggcacgaa ccccatcttc ttctgaaaaa tctgctttca tatagatagc gtctttttct
     4621 ttcatgatat tgtaaagttg ctcattaccc atgattacaa catcaatcgc acgttcttct
     4681 tcataatcaa aatgattttg gcgaagaaca gataagcgtt cgtcaggtcc taaagagata
     4741 tgaccagtac ttggttcaat atctcctgca aggattttaa gaaaagttga ttttccggca
     4801 ccattcgcac cgattaagcc gtaagtattg ccggctgtga atttgatatt aacatcatca
     4861 aatagttttc gatcactaaa gcgtagtgat acatcagaaa ctgtaagcaa tttgtcacct
     4921 catcttattt atctaagctt cattgtacct aaaatagtcc cctttttcaa tcagaaaagg
     4981 gctaatcact acataggcaa taaaacgtct ttgtcttcac gtttgtattg tttagtcaaa
     5041 ctaaatcctc ttcccataac ttgagctgct ggcatgacaa caataaaggc tgtgtcgtca
     5101 atttcgagga ttttttcttg gagagtgggt aattcgtgac ttgatacaat agccattaac
     5161 ataattttat cagaagtagt ataaccaccg cgaataggaa gtttagtcac tccacgatcc
     5221 ataacagttg tgatatattc tcgaatagct tgataatctt tggaaataat cataacattt
     5281 ttggatgagt caaagccatt ttccataacg ctaatcacat aaccaattac gaaaagtcca
     5341 atcgtagaat acatcacgtc atctgcagaa agtgcaataa atcccataag gacactaata
     5401 ccatctacaa ttgtcattgc aactccaaga gataaaggag agtatttatg aagtatttgt
     5461 gttagaattc cagttcctcc tgtcgaagag tttccccaaa aaaccattcc taaaccaatg
     5521 ccacagataa taccaccaaa aatagctgct aataactgat tgtgtgtcag agtaggtaat
     5581 gaattggttg aacggatggc aataggatat atccaagaac cataaagtgt tttaataaaa
     5641 ttttgttttc caagaaagaa gtaacacatt aataataagg gaatattact agccattaaa
     5701 aaaagcgacg gactaatgcc aaatagagct tttataacaa ctgcaatacc aaccataccc
     5761 cctgatgcaa tatgattgtc gacaaacatt gtattgaaac caatagctgt tatgaatgag
     5821 ccaattgtga caagtagtaa atcaatccct cgttttttca tgcttttaat ctcctttata
     5881 actggttaaa aatagtagac tcattatctc ttaaatgtac tgagaccgtc aagtcatatt
     5941 ttcaaaaaat ctggtgaact tgacaagaag tttaaaattt catagaataa atctaaatta
     6001 gaagagtatt cttttcatgt ttttagagag ttagtggttg gtgcaagcta acaacagaaa
     6061 aggtgaaatg gactaatgat tatttgaaag gaaaccataa ctttcaggtt ggcacccctt
     6121 accgtgcagc ttctttcatt gagaagattg agatagtatc tcgttttgat aaagcagatg
     6181 ctaattgagg tggtaccgcg tattacttgt aataacgccc tcacgtttta atagcgtggg
     6241 gactttttgc tatatcaaga gatagttagg gagaaaatga tgacaaaacc tattatttta
     6301 actggagata gaccaactgg aaaattacat ttaggtcatt atgtcggaag tcttaaaaat
     6361 cgtgtctttt tacaaaatga aaacaagtat aagatgtttg ttttcttggc cgatcaacag
     6421 gcactgacgg atcatgctaa agaatccgaa ttaattcaag aatctattgg aaatgttgcc
     6481 ttggattacc tctctgtcgg tttggatccg aagcaatcaa cgatttttat tcaaagtcag
     6541 attccagagc tagctgaatt gagcatgtat tatatgaatc tggtatcatt agcacgtttg
     6601 gaaagaaatc ccactgttaa aaccgaaatt gctcagaaag gttttggcga aagtattcca
     6661 tctggttttt tggtttatcc agtatcacag gccgctgata ttacagcatt taaagcgaat
     6721 ttagtacctg taggtaacga ccagaaaccg atgattgaac aaacacgtga aattgtgaga
     6781 agttttaatc atacttacca tacagactgt ttagtagaac ctgaaggtat ttatccagaa
     6841 aatgaaaaag ctggacgctt acctggtctt gatggcaatg ccaaaatgtc taaatcattg
     6901 ggaaatggaa tctatctctc agatgatgca gataccgttc gcaaaaaagt gatgagcatg
     6961 tatactgatc caaatcatat taaaatagaa gatcctggtc aaattgaagg gaatatggtc
     7021 tttcattatt tggatatttt tgctagaaaa gaagatcaag ctgatatcga agcaatgaaa
     7081 gagcattatc aaagaggtgg tttaggagat gtgaaaacga aacgctacct tctagatatt
     7141 ttagaacgtg aattagcacc tattcgtgaa agacgtttgg agtacgctaa agatatggga
     7201 gaggtgttcc gtatgttaca agaaggtagt caaaaagcaa gaactgtggc agccaagact
     7261 ttatcagaag tgaagtcagc aatgggtatt aattattttt aatgttttag agaaaaagct
     7321 aagttagtaa tgataagata attactatta tcaaagcaga acctaatttg tcatttcttt
     7381 acaggatagt agagattatg ttttatatag tcttattttt gattaaaaat aagacccaaa
     7441 tgaatttttt gattaataaa aaacggataa tactgatata ttatgctgat agtgacttca
     7501 gctgtaggaa tattttattg acaaatgatt ttagcgtgat atgatagtaa caataaaaaa
     7561 tagaccaggg taatattccc taaaaataaa agaaaagagg attatctgga tgtcaaattg
     7621 ggacactaaa tttttgaaaa aaggctacac ttttgacgat gtcttgctta ttccggcaga
     7681 gagtcatgtt cttccaaatg aagttgattt aaagacaaag ctcgcagaca atttgacatt
     7741 aaatattcct attattacag cagctatgga tacggttact ggtagtaaaa tggctattgc
     7801 aattgcgcgt gcgggtggtt taggtgttat tcataaaaac atgtctatta ctgagcaagc
     7861 agaagaagtt cgcaaggtaa aacgttctga aaatggtgtc atcattgatc cattcttctt
     7921 aacacctgaa cacaaggttt cagaagctga agaattaatg cagcgctacc gtatcagtgg
     7981 tgtgccaatt gttgaaactt tagcaaaccg taagttggtc ggtatcatta ctaatcgtga
     8041 tatgcgtttt atttctgatt acaatgcacc aatctctgaa catatgacta gtgagcacct
     8101 cgttacggct gctgttggta cagatcttga aactgcagaa cgtattcttc atgaacaccg
     8161 tattgaaaaa cttcctttgg ttgataatag cggtcgtctg tctggtttaa tcactatcaa
     8221 agatattgaa aaagtaatcg aattcccaca tgctgctaaa gatgaatttg gtcgtcttct
     8281 ggtcgctgct gcggtaggtg ttacctcaga tacttttgag cgtgccgagg ccttgtttga
     8341 agctggtgca gatgcgattg ttattgacac agctcatggt cattcggctg gtgttcttag
     8401 aaaaattgct gaaattcgtg ctcattttcc aaatcgtact ttgattgctg gaaatattgc
     8461 cactgctgaa ggtgcgcgtg ctctttatga tgcaggtgtt gatgttgtta aagtgggtat
     8521 tggtcccgga tctatctgta ccactcgtgt ggttgcaggt gtcggtgttc ctcaagtgac
     8581 tgctatttac gatgctgctg ctgttgctcg tgaatatggc aaaacgatta ttgctgatgg
     8641 tggtatcaag tattctggcg atattgttaa ggctcttgca gccggtggta atgctgttat
     8701 gcttggatca atgtttgcag gtactgatga agctccaggt gaaactgaaa tctaccaagg
     8761 ccgtaagttc aaaacttatc gtggaatggg ttccattgca gctatgaaga aaggatcaag
     8821 cgatcgttac ttccaaggtt ccgtcaatga agctaataag ttagttccag aaggtattga
     8881 aggccgtgtt gcttataaag gagctgcttc agatattgtc ttccaaatgt taggtggtat
     8941 ccgctcaggt atgggctatg ttggcgcagg tgacattcaa gaattacatg aaaatgctca
     9001 atttgttgaa atgtcaggtg caggcttgat tgaaagtcat ccacatgatg tccaaatcac
     9061 aaatgaagca ccaaactatt ctgtgcactg ataatttaat ttagtaactt taagggctga
     9121 catattgtgc cagctctttt tttatattaa aaaatgataa aatataataa caattttcgg
     9181 ataactttta ttgttttttc ttagtaaata agctataatt agtctttaca tgaaaccatt
     9241 ttatgataga attattataa tctttaaaga tttactataa aggagataac attttggaag
     9301 gtatttttta tgcacttatt cccatgttta catggggaag cattggattt gttagtaaca
     9361 aaattggagg aaaaccttcg caacaaactc taggaatgac ttttggggct ttattatttt
     9421 cattagctgt ttggttaatt gttagacctg agatgacact gcaactttgg atttttggga
     9481 ttttaggcgg ttttatctgg tcaattggtc aaactggtca atttcatgcc atgcaataca
     9541 tgggagtctc cgttgccaat cctttgtcaa gtggctctca acttgttctg ggaagtttga
     9601 ttggtgtcct cgtttttcat gaatggacaa aaccgatgca atttgtagta ggaagtctag
     9661 cactattact tctgattgtt ggtttctact tttctagtaa acaagatgat gctaatgcac
     9721 aagtcaatca ccttcataat ttttcaaaag gatttagggc gctcacttat tcaacaattg
     9781 gttacgtgat gtatgcagtc ttatttaata acatcatgaa atttgatgtt ttatctgtta
     9841 ttttaccaat ggcatttggt atggtcctag gagctataac tttcatgtca tttaaaattt
     9901 ccattgacca gtatgtgatt aaaaatagcg ttgttggatt actttggggc attgggaata
     9961 tttttatgct tttagctgca tcaaaagcag gacttgctat tgcctttagt ttctcacaat
    10021 taggtgctat catttcgatt gtcggaggca ttttattcct aggcgaaacc aaaaccaaaa
    10081 aagaaatgcg ttgggttgtc acaggaatta tttgttttat cgtaggtgct attttattag
    10141 gtgtggtcaa atcttaatcg ccacataata tcaaaaaaag gctcagttta actgaaccct
    10201 tttttgatgt tattagtgaa tatcagaatc aatttgaacg gttccttttg tcacgtgaaa
    10261 aatacggata ccttctggca actgtgacag gtgatccaaa cttgttgttg tgataaaggt
    10321 ttgaacattt tctttaatta ctgtttcaag taactttgtt tgtcgtgtat tgtctagctc
    10381 gctcatgaca tcgtctagta aaagaatggg attatctcca gtcaaggcct tcattaaact
    10441 aacttcagcc atttttaagg aaagaataag gctgcggtgc tgaccttgac tagcaaagtt
    10501 agcattcatc ccgttaatgt aaaaggctaa gtcatcgcga tgtgggccaa cgctagtgtt
    10561 tttacggaag aaatctttct gatgattttt ttcaagttgg tgtaaaaatt gttggtaaat
    10621 attagttttt ttgtcaaaga caactgacga ctgataggag agagagagac tttctaaacc
    10681 atttgagatg gcttggtgat gagtatttgc ttctttctct aacgcattga taaaatcaat
    10741 acgatgttcc atgacacggg ctccatagct agctaattgc tcatctagaa cagctagaaa
    10801 agcggcatca atttgttggg cactttttaa atagctatta cgctgtttaa gcacgtggtt
    10861 ataatgggat aactcagata agtaaacagg cttaatttga ccaaggtcaa tgtcgataaa
    10921 tttgcgacga aggctaggtg ctcccttaac tagttgcaaa tcttctggtg caaaaagcac
    10981 aaccatcatc gttccaatat aatcagataa ttttgcttgc tttagcgcat taatctttgt
    11041 tacacgtcct ttatctgata gattgatttc taaatcaaca gtaccactaa tacgctgaat
    11101 cttaccagta agagaaacag ttgagtgatc aaaatgaatc aattctttat cagccctcgt
    11161 gcgatgactt ctagtaagtg atagaaaata gattgcctcg agaaaattgg tttttccttg
    11221 agcattatta ccgataaaga catttaaacc cgacgaaaat gaggcaagca ggtggtcata
    11281 attgcgataa tgttttaact ctaactcttt aatccacata ctagatacct gggaagcgga
    11341 caggtgcagt tggtttacct ttagttttct tagttgctct tagtgatttt ttggtagtag
    11401 attgacgatt tttatgttta gaagaagtct ttttgttagc ttggttcatt tgtttcacta
    11461 gagccgctac acgtgtcttt tcagctattt cttcggcaaa ttgttctttt tcttcttgac
    11521 taggctcaac aatagtgata attaagtctt ggtcaggtag agaaatctta tcccctactc
    11581 tgattttttt gcctcgacgt ttctcatctt caccattaaa tagaacggtt gtttcagcga
    11641 gaaatctctt gatagcgcca ccactttgga taattcctaa ttctttaaga agggcttgta
    11701 gggtgataaa ttctgtaaat aatttgtata tcattgtcat ctccattttg tcattcaagt
    11761 cattatagca taaaacagaa ataaataata atagctgaat aagagctatt tataaagtat
    11821 catactcgtc caaataacta tttagttttg aagttcttgt taattagttt taacaaaagc
    11881 tgtacttatg tttatcattt ttctacgtat aaatcctatt attaatatgt ccctctagta
    11941 aagttgtata acacaaaact tttatatttt ggaacgttga caattttcaa gatataaact
    12001 taagttgcta taaaca
//
LOCUS       DAUCLR010000057        11884 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.057, whole genome shotgun sequence.
ACCESSION   DAUCLR010000057 DAUCLR010000000
VERSION     DAUCLR010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11884
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.057"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..837
                     /locus_tag="VGT87_001280"
     CDS             <1..837
                     /locus_tag="VGT87_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEQ3814132.1"
                     /translation="QVKDLVSGHLERIQEEVEKKYPNAGFNSDKYNGLKESNSLLSDG
                     FEVKSGNLIFDKKLTIPLTTLFDVINPDFLANSDRAAYDNYRTYKEQHPKKLVALTFD
                     DGPDPTTTPQVLDILAKYQAKGTFFMIGSKVVNNENLTKRVSDAGHEIANHTWDHPNL
                     TNLSVSEIQHQVNMTNQAIEKACGKKPRYLRPPYGATNATVQQSSGLTQMLWTVDTRD
                     WENHSTDGIMTNVKNQLQPGGVVLMHDIHQTTINALPTVMEYLKAEGYECVTVSELYA
                     HQ"
     gene            complement(947..2032)
                     /locus_tag="VGT87_001281"
     CDS             complement(947..2032)
                     /locus_tag="VGT87_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEQ3814133.1"
                     /translation="MITKFPPQWQEKLDQVAFTHLTPIQEQAFQPIVDGKNFLGISPT
                     GTGKTLAYVFPSLLALTPKKSQQLLILAPNTELAGQIFEVTKDWAQPLGLTAQLFISG
                     TSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMSVNTIVLDEYDELLGDSQYDFV
                     QKISHYVPRDHQMVYMSATNKVNQTSLAPNTFCIDLSEQTNDAIQHFYLMVDKRERTD
                     LLRKFANIPHFRALVFFNSLSDLGATEERLQYNGAAAVSLASDINVKFRKTILEKFKS
                     HQLSLLLATDLVARGIDINNLDYVIHFDVARDKENYTHRAGRTGRMGKSGIVITFVSH
                     PEDLKKLKKFAKVSEISLKNQQLHFIN"
     gene            2130..2756
                     /gene="udk"
                     /locus_tag="VGT87_001282"
     CDS             2130..2756
                     /gene="udk"
                     /locus_tag="VGT87_001282"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEQ3814134.1"
                     /translation="MLKKPIIIGVTGGSGGGKTSVSRAILDSFPNARIAMIQHDSYYK
                     DQSHMSFEERVKTNYDHPLAFDTDFMIQQLKELLAGRPVDIPIYDYKKHTRSNTTFRQ
                     DPQDVIIVEGILVLEDERLRDLMDIKLFVDTDDDIRIIRRIKRDMMERGRSLESIIDQ
                     YTSVVKPMYHQFIEPSKRYADIVIPEGVSNVVAIDVINSKIASILGEV"
     gene            2836..3099
                     /locus_tag="VGT87_001283"
     CDS             2836..3099
                     /locus_tag="VGT87_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEQ3814135.1"
                     /translation="MLLIWLGLLFVWNELVFVIYAFDKRKAIKKKRRISERNLLVITV
                     LFGGFGALLAAKKYHHKTRKWYFVITWYTSILLTLLVTYIFMK"
     gene            complement(3152..3961)
                     /locus_tag="VGT87_001284"
     CDS             complement(3152..3961)
                     /locus_tag="VGT87_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEQ3814136.1"
                     /translation="MKQITQQKWLRYGLFIALVLNGIELELLGLTANNLSFKEAFALI
                     LTISLLGIYLIPFAAAIFYLSKKFHMNLNVIIVSCLSGLYISGFLASCGNHLVGQFWS
                     YIIPSKDALKLWGDALTAPIVEEPIKASSAILVITLFPRLTLKEKLVVALLSGMGFQL
                     TEDIRYLIQAKSIDSLVPTAIERISTAVTSHWVHTAIFTIGAYLLLKGSNLFSKQQQI
                     FWLLSPLVLHFIWNSPLTSIPGMTVLLGTLILLIFGDLFQKINTLDDDVLF"
     gene            4106..4603
                     /locus_tag="VGT87_001285"
     CDS             4106..4603
                     /locus_tag="VGT87_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEQ3814137.1"
                     /translation="MNKSKKIEQYQLMIAQAKELFANESNALANLSNASALLNMTLPN
                     SVFAGFYLFDGQELILGPFQGRVSCVHIKLGKGVCGESAQSRRTIIINDVKQHANYIS
                     CDAAAMSEIVVPMVKEGHLIGVLDLDSSLVADYDEVDQEYLEAFVDLFLEKTTFTFNM
                     FGVKN"
     gene            4603..6273
                     /gene="dnaX"
                     /locus_tag="VGT87_001286"
     CDS             4603..6273
                     /gene="dnaX"
                     /locus_tag="VGT87_001286"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEQ3814138.1"
                     /translation="MYQALYRKYRSQTFDEMVGQSVISTTLKQAVESGKISHAYLFSG
                     PRGTGKTSAAKIFAKAMNCPNQVDGEPCNQCDICRDITNGSLEDVIEIDAASNNGVDE
                     IRDIRDKSTYAPSRATYKVYIIDEVHMLSTGAFNALLKTLEEPTENVVFILATTELHK
                     IPATILSRVQRFEFKAIKQKAIREHLAWVLDKEGIAYEVDALNLIARRAEGGMRDALS
                     ILDQALSLSPDNQVTIAIAEEITGSISILALGDYVRYVSQEQATQALAALETIYDSGK
                     SMSRFATDLLTYLRDLLVVKAGGDNQRQSAVFDTNLSLSIDRIFQMITVVTSHLPEIK
                     KGTHPRIYAEMMTIQLAQKEQILSQANLSGELISEIETLKNELAQLKQQLSQLQSRPD
                     SLARSDKTKPKTTSYRVDRVTILKIMEETVRNSQQSRQYLDALKNAWNEILDNISAQD
                     RALLMGSEPVLANSENAILAFEAAFNAEQVMSRNNLNDMFGNIMSKAAGFSPNILAVP
                     RTDFQHIRKEFAQQMKSQKDSVQEEQEVALDIPEGFDFLLDKINTIDD"
     gene            6368..6571
                     /locus_tag="VGT87_001287"
     CDS             6368..6571
                     /locus_tag="VGT87_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEQ3814139.1"
                     /translation="MTIRQFLFMAFVCAFETYFFNDLLLSGNYLFALFWGLLLFRDLR
                     RVHTINQLTKTILKTANSPKKKD"
     gene            complement(6546..7487)
                     /gene="birA"
                     /locus_tag="VGT87_001288"
     CDS             complement(6546..7487)
                     /gene="birA"
                     /locus_tag="VGT87_001288"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HEQ3814140.1"
                     /translation="MKTSEKIYQLLSQTDDFVSGEYLADQLSISRTSVWKSIKSLENQ
                     GIQIDSLKHKGYRMVQGDILLPKTISQGLGMPVTYTPHSQSTQLDAKQGIEAHNSAPR
                     LYLAPSQEAAKGRLDRQFFSASTGGIYMSMYLKPNVPYADMPPYTMMVASSIVKAISR
                     LTGIDTEIKWVNDIYLGNHKVAGILTEAITSVETGLITDVIIGVGLNFFVTDFPEAIA
                     QKAGSLFTEKPTITRNDLIIDIWKLFLSIPVKDHVKVYKEKSLVLNKQVTFIENSQEK
                     RAIAIDLTDQGYLIVQFENGDLQTLRSGEISLSSWEN"
     gene            7691..10069
                     /locus_tag="VGT87_001289"
     CDS             7691..10069
                     /locus_tag="VGT87_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEQ3814141.1"
                     /translation="MKTKKVIILVGLLLSSQLTLIACQSRGNGTYPIKTKQSCKGMTS
                     NKIKPIKKSKKTNKTHKGVAGVDFPTDDGFILTKDSKILSKTDQGIVVDHDGHSHFIF
                     YADLKGSPFEYLIPKGASLAKPAVAQRAASQGTSKVADPHHHYEFNPADIVAEDALGY
                     TVRHDDHFHYILKSSLSGQTQAQAKQVATRLPQTSRLVSTATANGIPGLHFPTSDGFQ
                     FNGQGIVGVTKDSILVDHDGHLHPISFADLRQGGWAHVADQYDPAKKAEKPAETHHTP
                     ELSEREKEYQEKLAYLAEKLGIDPSTIKRVETQDGKLGLEYPHHDHAHVLMLSDIEIG
                     KDIPDPHAIEHARELEKHKVGMDTLRALGFDEEVILDIVRTHDAPTPFPSNEKDPNKM
                     KDWLATVIKLDLGSRKDPLQRKGLSLLPNLETLGLGFTPIKDISPVLQFKKLKQLLMT
                     KTGVTDYRFLDNMPQLEGIDISQNNLKDISFLSKYKNLTLVAAADNGIEDIRPLGQLP
                     NLKFLVLSNNKISDLSPLASLHQLQELHIDNNQITDLSPVSHKESLTVVDLSRNADVD
                     LATLQAPKLETLMVNDTKVSHLDFLKNNPNLSSLSINRAQLQSLEGIEASSVIVRVEA
                     EGNQIKSLVLKDKQGSLTFLDVTGNQLTSLEGVNNFTALDILSVSKNQLTNVNLSKPN
                     KTVTNIDISHNNIPLADLKLNEQHIPEAIAKNFPAVYEGSMVGNGTAEEKAAMATKAK
                     ESAQEASESHDYNHNHTYEDEEGHAHEHRDKDDHDHEHEDENEAKDEQNHAD"
     gene            10606..11802
                     /locus_tag="VGT87_001290"
     CDS             10606..11802
                     /locus_tag="VGT87_001290"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEQ3814142.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAEDPEAHVAAE
                     TCVYTGSVHVFGEISTTAYIDINRVVRDTIAEIGYTEAEYGFSAESVGVHPSLVEQSG
                     DIAQGVNEALESREGDTDDLSHIGAGDQGLMFGFAINETPELMPLPISLSHQLVRRLA
                     ELRKSGEISYLRPDAKSQVTVEYDEHDKPVRVDTVVISTQHDPEATNDQIRQDVIEKV
                     IKAVIPADYLDDDTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFSG
                     KDATKVDRSASYAARYIAKNLVAAGLATKAEVQLAYAIGVAQPVSVRVDTFGTSTVPE
                     AVLEAAVRQVFDLRPAGIIQMLDLKRPIYKQTAAYGHMGRTDIDLPWERLNKVDALVE
                     AVKTVL"
BASE COUNT         3801 a         2077 c         2452 g         3554 t
ORIGIN      
        1 caggtaaagg atctagtatc aggacattta gaaagaatac aagaagaagt tgaaaaaaaa
       61 tatccaaatg ctggttttaa tagcgataag tacaatggct taaaagaatc taattcttta
      121 ttaagcgatg gctttgaggt aaaatcggga aaccttattt ttgataaaaa gctaacgata
      181 cctttgacga cattatttga tgttattaat ccagattttt tagcaaatag cgatagagct
      241 gcgtatgata attataggac ctacaaagaa cagcatccca aaaaactagt tgcattaacg
      301 tttgatgatg gtccagatcc gacgacgact cctcaagttt tagatatttt ggcaaaatac
      361 caggctaagg gaactttctt tatgataggt tcaaaagttg tgaataatga aaaccttact
      421 aaacgtgtta gcgacgctgg ccatgaaatt gctaatcata cttgggatca tcccaatctg
      481 actaatcttt cagtcagcga gattcaacat caagttaata tgacgaacca ggctattgaa
      541 aaagcttgtg gcaagaaacc tcgctattta cgccctccat acggagctac aaatgcaact
      601 gttcagcaat cctcaggatt aacacaaatg ctttggacgg ttgatacaag agattgggaa
      661 aatcatagca ctgatggcat tatgactaat gtcaaaaacc aattgcaacc gggaggagtt
      721 gttttaatgc atgatattca tcaaacaact atcaatgctt tgccgacagt tatggaatat
      781 ctaaaagcag aaggatacga atgtgtgact gtatcagaac tctatgcgca tcagtaagta
      841 atatcgttat gtttctttag aattgatata ctaaacaatc caattacttt gaagtaattg
      901 gattgttgtt tttagtagcc cttgattaag ttttgttttt taacaactag ttgatgaaat
      961 gaagttgttg atttttgagg gagatttcag agactttagc aaattttttc aattttttga
     1021 ggtcttcagg gtggctaaca aaggtaataa cgataccaga ttttcccata cggccggttc
     1081 gtccagctct gtgagtataa ttttccttat cacgagcaac atcaaaatga atgacatagt
     1141 caagattatt aatatcaatg ccccgagcaa caagatccgt agctaacaat aaggagagtt
     1201 ggtgggattt gaatttttcg agaatggttt tacggaattt gacattgatg tcactcgcta
     1261 aggaaaccgc agcagctcca ttatactgga gacgttcttc cgtagcaccc aaatcagata
     1321 aactgttgaa aaagaccaaa gctctaaaat gaggaatatt agcaaatttt cgtaataaat
     1381 ctgtacgttc gcgcttgtca accatgaggt aaaaatgctg aatagcatcg ttggtttgct
     1441 ctgaaagatc gatgcagaat gtatttggag ctaaggaagt ttgattcacc ttattagtag
     1501 cactcatgta taccatttga tgatctcgag gaacatagtg gctaattttt tgaacaaaat
     1561 catattggga atcacctaat agttcgtcat attcatctaa aacaatggtg ttgacagaca
     1621 tcatcttgat ttttttcaat ttaatcagtt caaaaatgcg tccaggggtc ccaatcagga
     1681 tttcaggacc ttttttgagg cgttcgattt gacgtttttg actggttcca gaaataaaga
     1741 gttgtgccgt taagcctagt ggctgtgccc aatcctttgt gacttcaaaa atttggccag
     1801 ctaattcggt attaggggct aaaatgagta gttgttggga ctttttaggc gtcagtgcca
     1861 atagactagg aaagacataa gctaaggtct tgcccgtccc agtagggcta ataccaagga
     1921 aatttttacc atcaacaata ggttgaaagg cctgttcttg gattggcgtt aaatgggtaa
     1981 atgcaacttg gtcaagtttt tcttgccatt gagggggaaa tttagtgatc atgcgtcttc
     2041 cttttactat tgtttctata ttcattatag cattatagtc tcaattttgg taaaataatg
     2101 agaaagactt tttagatttg gagtttaaaa tgcttaaaaa accgattatt attggtgtaa
     2161 ctggtggttc aggtggcggt aaaacaagtg tgtcacgcgc tattttggac agttttccaa
     2221 atgctcgtat tgcaatgatt caacacgatt cttactataa agatcagtct cacatgagtt
     2281 ttgaagaaag ggtcaagacc aattatgacc atcctctagc ttttgataca gattttatga
     2341 ttcagcaact aaaagaatta ttagctggta gacctgtaga tattcctatc tatgattaca
     2401 aaaagcatac ccgcagtaat acgacctttc gtcaggatcc tcaggatgtt attattgttg
     2461 aaggtattct tgtcctagaa gatgagcgtt tgcgagattt gatggatatc aagctttttg
     2521 tagacacaga tgacgatatt cgtattattc gtcgtattaa gcgtgacatg atggaacgtg
     2581 gcagaagctt agaaagtatt attgatcagt acacaagcgt ggtaaagcct atgtatcatc
     2641 agtttattga gccaagtaaa cgctatgctg atattgttat tccagaggga gttagtaatg
     2701 tcgttgctat tgatgttatt aatagtaaaa ttgcgagtat tcttggagaa gtatagtgga
     2761 agtggctgag tagattattt aaatctgctc ttttattgtt gtgctcttca tagagaaagg
     2821 gatcataaaa tagaaatgtt attaatttgg cttggattac tatttgtttg gaatgaactc
     2881 gtgtttgtca tatatgcctt tgataaaaga aaagccataa aaaagaaacg gcgtattagt
     2941 gaaagaaact tattagtgat aacagttttg tttggaggtt ttggagcatt acttgcagct
     3001 aaaaagtacc accataaaac cagaaagtgg tactttgtca tcacttggta tactagtatt
     3061 ttgttgacat tactagtaac ttatatcttt atgaagtgag tcacttaaaa gacccttaat
     3121 cagtattatt tagtcaaggc tcttttatag tttaaaaaag cacatcatcg tcaagggtgt
     3181 tgattttttg aaaaagatca ccaaagatta aaaggattaa tgttcctagt aaaactgtca
     3241 tgccaggaat gcttgttaga ggagagttcc agatgaaatg tagcaccaga ggacttaata
     3301 accagaaaat ttgttgctgt tttgaaaaaa ggttagaacc ctttaataat aagtatgctc
     3361 caatggtaaa aatagctgta tgtacccaat gtgaagtcac agcagtgcta atacgttcaa
     3421 tagcagtcgg tacgagacta tctatagatt ttgcttgaat caaataacgg atatcttctg
     3481 ttagttgaaa gcccatgcca gaaagtaaag caacaactaa tttttcttta agagtaagtc
     3541 taggaaataa agtaattact aaaatagccg aagaagcctt aatgggctct tcgacaatag
     3601 gggcagttaa agcatctccc caaagcttta aagcgtcttt agaagggatg atgtatgacc
     3661 aaaattgtcc tacgagatgg ttcccacaag aagctaaaaa acctgaaata taaagtccgc
     3721 taagacagct aacaataata acattcaggt tcatatgaaa ttttttactt aaataaaaaa
     3781 tagctgctgc aaatggaatt aagtaaatgc ctaatagact aatggttagt attagagcaa
     3841 aagcttcttt aaaggaaagg ttgttagctg tcagtcctaa caattcaagt tcaataccat
     3901 ttaaaactaa agcaataaaa agaccgtatc gtaaccattt ctgttgagtg atttgtttca
     3961 cgaaaacctc ctaatattaa gatgatatta ataatattga aaattatatc ataaacaacc
     4021 tcaaaaacca ttttaaaaac tgactgaagc gaaagatagt cgtaaaaaat actcgaatag
     4081 acccagatat gctataataa aaggtatgaa taagtctaaa aaaattgagc aataccaact
     4141 aatgatagca caggcaaaag agctgtttgc gaatgaatca aatgctttgg ctaatttgtc
     4201 aaatgccagc gccctgttaa acatgacact acccaattct gtttttgcgg gattttacct
     4261 ttttgatggg caggaattaa ttcttggccc ttttcaaggt agggtttctt gcgtccatat
     4321 caagcttggt aaaggtgttt gtggcgaatc agctcagtct cgtcgaacca ttattataaa
     4381 tgatgtgaag cagcatgcca actatatttc ctgtgatgcg gcagccatga gtgaaattgt
     4441 tgtgcctatg gtgaaagaag gccatttgat tggggtgctg gacttggatt ctagccttgt
     4501 agctgattat gatgaggttg atcaagaata cttagaggcc tttgtggacc tatttcttga
     4561 aaagaccacg tttaccttta acatgtttgg agttaaaaac tgatgtatca agctctttat
     4621 cggaaatacc ggagccaaac gtttgacgaa atggtgggac aatcggttat ttccacaact
     4681 ttaaagcagg cagttgaatc tggcaagatt agccatgctt atcttttttc aggtcctaga
     4741 gggactggga aaacaagtgc ggcaaagatt tttgcaaagg ccatgaattg tcctaaccaa
     4801 gtcgatggtg aaccctgtaa tcaatgcgat atttgccgag atatcacgaa tggaagctta
     4861 gaagatgtga ttgaaattga tgctgcctcg aataatggtg ttgatgaaat tcgtgacatt
     4921 cgagacaaat caacctatgc gccaagtcgt gcgacttaca aggtttatat tattgatgag
     4981 gttcacatgt tatcaacagg ggcttttaat gcgcttttga aaactttgga agaaccgaca
     5041 gaaaatgttg tctttatctt ggcaacaacg gaattgcata aaattccagc cactatttta
     5101 tctcgtgtgc aacgctttga attcaaagct attaagcaaa aagctattcg agagcattta
     5161 gcctgggttt tggacaaaga aggtattgcc tatgaggtgg atgctttaaa tctcattgca
     5221 aggcgagcag aaggaggcat gcgtgatgct ttatctattt tagatcaggc tttgagcttg
     5281 tcaccagata atcaggtcac cattgcaatt gccgaagaaa ttacaggttc tatttccata
     5341 cttgctctgg gtgactatgt tcgatatgtc tcccaagaac aggctacgca agctctggca
     5401 gccttagaga ccatttatga tagtgggaag agcatgagcc gctttgcgac agatttattg
     5461 acctatctgc gtgatttatt ggtggttaaa gctggcggcg acaatcaacg tcagtcagct
     5521 gtttttgata ccaatttgtc tctctcgata gatcgtatat tccaaatgat aacagttgtt
     5581 actagtcatc tccctgaaat caaaaaggga acccatcctc ggatttatgc cgaaatgatg
     5641 accatccaat tagctcagaa agagcagatt ttgtcccaag caaacttgtc aggagagtta
     5701 atctcagaga ttgaaacgct caaaaatgag ttggcacaac ttaaacaaca attgtcgcag
     5761 ctccaatcgc gtcctgattc actggcaaga tctgataaaa cgaaacctaa aaccacaagc
     5821 tacagggttg atcgggttac cattttgaaa atcatggaag aaacggttcg aaatagccaa
     5881 caatctcgac aatatctaga tgctctaaaa aatgcttgga atgaaattct agataacatt
     5941 tctgcccaag acagagcctt attgatgggc tctgagcctg tcttagcaaa tagtgagaat
     6001 gcgattttgg ctttcgaggc tgcctttaat gcagaacaag tcatgagccg aaataatctt
     6061 aatgatatgt ttggtaacat tatgagtaaa gctgctggtt tttctcccaa tattctggca
     6121 gtaccaagga cagattttca gcatattcgt aaggaatttg ctcagcaaat gaaatcgcaa
     6181 aaagacagtg ttcaagaaga acaagaagta gcgcttgata ttccagaagg gtttgatttt
     6241 ttgctcgata aaataaatac tattgacgac taaaactgaa atacataagg gtaaggtttt
     6301 gcccttaccc acgttttatc tcgttacttt tcttcatagt acagtatttt tttgttataa
     6361 taaagctatg actattcgac aatttctttt tatggccttt gtatgtgcct ttgaaaccta
     6421 tttttttaat gacctgttgc tctctggaaa ttatctcttc gccttatttt ggggcttgtt
     6481 gctctttaga gatttaagaa gagtgcatac cattaaccaa ttgactaaaa ccattttaaa
     6541 aacggctaat tctcccaaga agaaagactg atttcaccgc ttcgcaaggt ttgaaggtca
     6601 ccattttcaa actgtacaat gaggtatcct tgatctgtga gatcaatcgc gatagcacgt
     6661 ttttcttgac tattttctat aaaggtcact tgtttattaa gaacaagtga tttttctttg
     6721 taaactttga catggtcctt gacaggaata gataagaata atttccagat gtcaatgata
     6781 aggtcattac gcgtgatagt cggtttttcg gtaaagagag aacctgcctt ttgagcaatc
     6841 gcttctggga agtcggtgac aaaaaagttg agaccaaccc caataatgac atcggtgatt
     6901 aaaccagttt caacagaagt aatggcttct gtcagaatcc ctgcaacttt atggttacct
     6961 agatagatgt cgtttaccca tttgatttct gtatcaatgc ctgttaatcg cgagatagct
     7021 ttgacaatac tggaagcaac catcatcgta taaggaggca tatcagcata agggacattg
     7081 ggttttaggt acatggacat gtaaatacca ccggtgctag ctgaaaaaaa ttgccgatcg
     7141 aggcgtcctt tggcagcttc ttggctagga gctaggtaaa gccttggagc gctgttatga
     7201 gcttcgattc cttgtttggc atctaattgg gtagattggc tatgaggggt ataggtgact
     7261 ggcatcccaa gcccttggct aatggttttg ggtaataaaa tatcaccttg taccatgcgg
     7321 tagcccttgt gttttaaaga atcaatttga atcccctgat tttcgagact tttgatggat
     7381 ttccaaacgg atgtacgaga gatactgagt tgatctgcta gatattcccc actgacgaag
     7441 tcatcggttt gtgacagtaa ttgataaatt ttttctgatg ttttcatatg tatagtatat
     7501 tataaaagat cttattttca aaggaaaaga aaagttagtt tttttgtgta atgctactaa
     7561 caacagaaac tattgtttgt tgaagcttca gagctccaac tcgtcaacaa ctaatagtta
     7621 tcctaaaaag agcttgagga aaaacaagtg atgtgatata cttaaccagt taaataaaaa
     7681 ggagggttga atgaaaacga aaaaagttat tattttagtt ggtctattgt tatcatctca
     7741 gttgactttg atagcttgtc aatcacgagg taatggtaca tatcccatta aaacgaaaca
     7801 atcatgtaag ggaatgacgt caaacaaaat taaaccgatt aaaaaaagca aaaagacaaa
     7861 caagactcac aaaggtgtgg cgggtgtcga ttttcctaca gatgatgggt ttattttaac
     7921 caaagactca aaaatcttat caaaaacaga tcagggaatc gttgttgacc atgatggtca
     7981 ttcgcatttt attttttatg ccgatttaaa gggaagtcca tttgaatacc ttattccaaa
     8041 aggagcaagt ttagctaagc cagctgttgc tcagcgagca gctagtcaag ggacttctaa
     8101 agtagcagat cctcatcacc attatgaatt taacccagcg gatattgtgg ctgaagatgc
     8161 tttaggctac acggttcgcc acgatgatca cttccattat attttgaagt caagcttatc
     8221 aggtcagaca caggcacaag ctaaacaggt tgctactcgc ttgccacaaa ccagtagact
     8281 tgtttcaaca gctacagcta atggtattcc aggcttgcat ttcccaacct cagatggttt
     8341 tcaatttaac ggtcaaggta ttgttggggt aacaaaagac agtattttag tggaccacga
     8401 tggtcactta catcctattt cttttgcgga ccttcgtcag ggtggctggg cacatgtggc
     8461 agatcaatac gatcccgcta aaaaagcaga aaagccagca gaaacccatc acacaccaga
     8521 gctatctgaa cgtgaaaagg aataccaaga aaaattagct tatttggcag aaaaattggg
     8581 gattgatcca tcaactatta aacgtgtgga aacacaagac ggtaaacttg gtttggaata
     8641 ccctcaccat gaccacgcac acgtattgat gttatctgat attgaaatcg gaaaagacat
     8701 tccagatcca catgctattg agcatgcccg tgaattggaa aaacataagg ttggaatgga
     8761 taccttgcgt gccttagggt ttgatgaaga agtgattttg gatatcgttc gcactcacga
     8821 tgctccaacc ccattcccat caaatgaaaa agatccgaat aagatgaaag actggttagc
     8881 aacggttatc aaacttgact tgggcagccg taaagatcct ttgcaacgta aaggactttc
     8941 actgttaccc aacttagaaa ctttaggact tggctttaca ccaatcaaag atatctcacc
     9001 tgttttgcaa tttaaaaaat tgaaacagtt gttaatgaca aaaacagggg tgactgatta
     9061 tagatttttg gataatatgc cacagttaga aggcattgat atttcacaaa acaatctcaa
     9121 agatattagt ttcttgagca aatataaaaa cttaactcta gtagcggctg ctgataatgg
     9181 tattgaagat attaggccgc ttggtcaatt accaaatctc aaattcctcg tattgagtaa
     9241 taataagatt tctgatttaa gcccactggc atcgttacat caattgcaag aattgcacat
     9301 tgataataat cagattacag atttaagccc tgtttctcat aaagaatcat tgacggttgt
     9361 tgatttatca agaaatgctg atgttgactt agcaacactt caagcaccca aattagaaac
     9421 gttaatggtc aatgatacca aggtttctca tttggatttc ttgaaaaata atcctaatct
     9481 atctagccta tctattaacc gtgcgcaatt gcaatctctt gaaggtattg aagcaagtag
     9541 cgtcattgtc agagtagaag cagaaggtaa ccaaattaaa tcgcttgtgc ttaaagacaa
     9601 gcaagggtca cttactttct tggatgtgac aggcaaccag ttgacttctc tagaaggtgt
     9661 taataatttt acagcacttg acattttaag cgtgtctaaa aaccaattaa caaatgtcaa
     9721 cttatctaaa cccaataaga cagttactaa cattgatatt agtcataaca atatcccatt
     9781 ggcagacctt aaattgaacg agcaacatat tccagaagcc attgcgaaaa acttcccagc
     9841 ggtttacgaa ggttctatgg taggtaatgg aacagctgaa gaaaaagcag ctatggctac
     9901 taaggcgaaa gaaagtgctc aagaagcatc ggaatcacat gactacaacc ataatcatac
     9961 ctatgaagat gaagaaggtc atgctcacga gcacagagac aaagatgatc acgaccatga
    10021 acatgaggat gaaaatgaag ctaaagatga gcaaaaccat gctgactaaa ctaatctaag
    10081 aataacccga tccaactgaa tcgtcctaat gacaccattg ttgactatca agagaacctc
    10141 aagaataacc ttgaggtttt tttgaaataa ataggataat gagtgaaaag aatggcacct
    10201 aaatggctaa aacatgttat aatagattgt tattgagtcc cgataagacg gtctgttgaa
    10261 cgaaatgttt ttgagacgtt gttgacttca tttggggaac agtcgttctc cctttagcta
    10321 aattacttcg tctaaggctt attttgtggt gacatcctct caacaaggag accggattct
    10381 ttgtaactca acgctattgt cagacattct ttgtctggtt aatggacgct agccactagg
    10441 tggactagct agttgcctta ctagtaaaac cgtaactgat tagctaagtt ggttttggcc
    10501 acttgacttg ctcaatcagc taggaagaaa ggggtaggca acttcttgct ttgtggtcaa
    10561 tgtgactgaa aaaggaagaa tcaacatatt aggggggaca tttttatgtc agaacgtaaa
    10621 cttttcacgt ctgagtccgt atctgaaggg catccagata agattgcaga ccaaatttca
    10681 gatgctattt tagatgccat tttagcagaa gatccagaag cccatgtggc tgcagaaact
    10741 tgtgtttaca caggttctgt tcatgttttt ggagaaatct caacaacagc atacattgat
    10801 attaaccgtg tggtccgcga caccattgca gaaattggtt atacagaagc agaatatggc
    10861 ttttcagcag aatcagtggg agtacatcca tcacttgtag aacaatctgg agatatcgct
    10921 cagggggtta atgaagctct cgaaagcaga gaaggtgata ctgatgacct tagccatatt
    10981 ggagcgggtg accaagggct catgtttggt tttgccatca atgaaacacc agaattgatg
    11041 cctttaccaa tttccttatc tcaccaatta gtgcgtcgct tggcggaatt acgtaagtca
    11101 ggtgagatta gttatctacg tccagatgct aaatctcaag tgacagttga gtacgatgaa
    11161 cacgataaac ctgttcgtgt ggatacagtc gttatttcta ctcagcatga tccagaagct
    11221 actaatgacc aaattcgtca ggatgtgatt gaaaaagtga tcaaggctgt aattccagca
    11281 gattatttag atgatgacac aaagttcttc attaatccaa caggacgttt tgttattgga
    11341 ggtccgcaag gagactctgg attgaccgga cgtaagatta ttgtggatac ctatggaggc
    11401 tattcacgtc atggtggcgg tgctttttca ggaaaagatg cgacaaaagt ggaccgttcg
    11461 gcttcttacg ctgctcgtta tattgctaaa aaccttgtgg cagcaggtct tgcgacgaaa
    11521 gctgaagtcc aactagctta tgcgattggg gtggctcagc ctgtgtctgt tcgcgtggat
    11581 acatttggaa cctcaacggt tcctgaagct gttttagaag ctgctgttcg tcaagtcttt
    11641 gatttacggc cagcaggcat tattcaaatg cttgatttaa aacgcccgat ttacaaacaa
    11701 acagctgcct atggtcacat ggggagaaca gatattgatt tgccatggga gcgtttaaat
    11761 aaggttgatg ctttagtaga agctgtaaaa acagtattat aaacgacaac tttattaaaa
    11821 aagtagagtg agtgaaaaaa tcttactctg cttttttgtt attaaataag cctttaaaag
    11881 ggat
//
LOCUS       DAUCLR010000058        11749 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.058, whole genome shotgun sequence.
ACCESSION   DAUCLR010000058 DAUCLR010000000
VERSION     DAUCLR010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11749
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.058"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            149..1357
                     /locus_tag="VGT87_001291"
     CDS             149..1357
                     /locus_tag="VGT87_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ3814143.1"
                     /translation="MNNRKKNIQYLVYSKVIYRIGDVMFDFANNTFLAGLNPASLSLV
                     AVYQFLESVIGVLFNLFGGVIADSFKRKKIIITTNILCGTACLVLSFLTKEQWLVYAI
                     VLTNVILAFMSAFSSPSYKAFTKEIVKKDSISQLNSLLETTSTVIKVTVPMVAIFLYK
                     LLGIHGVLLLDGLSFLIAALLISFILPVNDEVVIKEKVTIREIFNDLKIGFKYVYSHK
                     SIFIITVLSALVNFFLAAYNLLLPYSNQMFGEISAGLYGTFLTAEAIGGFIGAILSGF
                     VNKELSSMRLILFLSLSGLMLMLAPPFYIMFHNAIILALSPALFSLFLSIFNIQFFSL
                     VQKDVDNDFLGRVFGIIFTITILFMPIGTGFFSVALNPNNSFNLFIIGSCITILSLVF
                     RILFKKYNIR"
     gene            complement(1459..1668)
                     /locus_tag="VGT87_001292"
     CDS             complement(1459..1668)
                     /locus_tag="VGT87_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ3814144.1"
                     /translation="MRFSYNKLWKLLIDKGWTKTKLRQEAGISSSTIAKLGKGENITT
                     DILLEICIALDCKIEDIVEIVDNNI"
     gene            complement(1658..3315)
                     /locus_tag="VGT87_001293"
                     /pseudo
     CDS             complement(1658..3315)
                     /locus_tag="VGT87_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904398.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2326 domain-containing protein"
     gene            complement(3306..3530)
                     /locus_tag="VGT87_001294"
     CDS             complement(3306..3530)
                     /locus_tag="VGT87_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814145.1"
                     /translation="MQLPNKLYSYQKSTLAYLPRVLNEIKSGNSNVKDIFHAISEELD
                     DPTDFLSIMDCLYALNAIEMSNEGEVKLCL"
     gene            complement(3530..4423)
                     /locus_tag="VGT87_001295"
     CDS             complement(3530..4423)
                     /locus_tag="VGT87_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814146.1"
                     /translation="MTTMLDFPFHHEFIESLGRWTHILITLPKRHHGQVDTAIPRYLL
                     VTIREKTGIVKLTTVQNTAFTTQSNQLKKQYGQFLLTESNNCCACPGCDRPLILTSGN
                     LAAENYEVSAIEKDKDAEPLNLIALCPDCFLTYQADSRKKIVTALKNVKKILVSAHNS
                     QQAISDMKLDSGIVSVLTSLNKLKCDEYDISYDSKQLTDKISPENNRTLYQLVKNPVI
                     DNYLTIQKIIVNLDKQGKIDYEEIQYQTRSMYKKLKAAKHNNLAIFNTISEKLHKATL
                     QDIYFCQMIVSYFIQKCEVLE"
     gene            4439..4615
                     /locus_tag="VGT87_001296"
     CDS             4439..4615
                     /locus_tag="VGT87_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEQ3814147.1"
                     /translation="MGQNVVMRQAPAGNIKPDKEKSVEKIDGIVSLILGLDRCIRRQG
                     DEASVYDERGILSF"
     gene            4670..4972
                     /locus_tag="VGT87_001297"
     CDS             4670..4972
                     /locus_tag="VGT87_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEQ3814148.1"
                     /translation="MAKTGTLNLRVDDSVKSAADDILKRLGIPMSTAIDMFLNQIILT
                     GGIPFDVSLPEAPQRVNVDYMSQEKFYDKLITSFEDAKTGNRQDVREFLFQLKENA"
     gene            4972..5277
                     /locus_tag="VGT87_001298"
     CDS             4972..5277
                     /locus_tag="VGT87_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEQ3814149.1"
                     /translation="MKEYQVTMSDDAKADLLSIYHYVRDELCAPQAADNLLERLSQGM
                     LSLSIFPERCAIIEDLIGKGYTFRQLIVKKYRIVYHVLEDEVIIVAVVYGSRHMNNW"
     gene            5296..5516
                     /locus_tag="VGT87_001299"
                     /pseudo
     CDS             5296..5516
                     /locus_tag="VGT87_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424370.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5621..6072
                     /locus_tag="VGT87_001300"
                     /pseudo
     CDS             5621..6072
                     /locus_tag="VGT87_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407405.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            6138..6446
                     /locus_tag="VGT87_001301"
     CDS             6138..6446
                     /locus_tag="VGT87_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814150.1"
                     /translation="MTRLISQQILSEYVKSLEVEADKVVIPGQNTPVFSWDRDGNLSI
                     DTPLLKVRGESLATQADLTTISLTPGPKGELWQTSVERLDIKVDKHISLTFKNGVRID
                     "
     gene            6851..7189
                     /locus_tag="VGT87_001302"
     CDS             6851..7189
                     /locus_tag="VGT87_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEQ3814151.1"
                     /translation="MKSPFTVTNTMLNKVVEISKIIGNLELQVQKDLKLRKENRIQSI
                     HSSLAIEQNSLTVEQITAIIDGKRVLGNPREIREVKNAYEAYEEILTLTPYDESHFLK
                     MKEFQQYIYR"
     gene            7213..8037
                     /locus_tag="VGT87_001303"
     CDS             7213..8037
                     /locus_tag="VGT87_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine ligase"
                     /protein_id="HEQ3814152.1"
                     /translation="MGLGSDSLPVKISLFDVDTYLADSMQFHLEYILRLFPKSGVHYL
                     MPTFRGEAVDHRHLSQFYHSECEIEGDLHDVMNLVDRYIKFLVSEILRECRDSIFAIT
                     KDVSHLESFIDRKIPIITFEEACTILENEIDSLEYNDEHHFYVLTDVGEKKLMEHFEG
                     VVWVKNYPCKSVPFYQRFSTDHLFAYNADLLLGIGETVGCGERCETYEEVVENMNYLQ
                     VDASEYSWYLEMKKTRPMKSSGFGLGVERLIAWILKCDDVRKVQLISRENGIDYLP"
     gene            8051..9295
                     /locus_tag="VGT87_001304"
                     /pseudo
     CDS             8051..9295
                     /locus_tag="VGT87_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285438.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit family protein"
     gene            9220..10239
                     /locus_tag="VGT87_001305"
     CDS             9220..10239
                     /locus_tag="VGT87_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEQ3814153.1"
                     /translation="MFGKQGIRCARKGDERNIKIIVNRVNSERNIKSIGICSPSYPGT
                     FHYQKRYKRGVEFLQNKGFTIVEGNLTGKEGYRGEISGGVQDRAEELNQLIPRVDVIM
                     TSIGGYNSNSILKYIDYDLFKQKSPIFIGYSDTTALALALYNKTGCITYLSQSVISNF
                     GEFEPFNELNYFYFDFMLQSKCETLMVQIPDVWTDEWINWETYERTKKTNKNEWIIFN
                     KGEFNGTLIGGNLDTIVGIIGTEYMPKITEDTILLLEDVYTDLGRLYRNFTTLALHGI
                     FDKIGGLIISKFETIGENSDVINDIINEFVGHRKIPILLNFDCGHTHPSCLMPIGGKI
                     TLSLS"
     gene            10252..10452
                     /locus_tag="VGT87_001306"
     CDS             10252..10452
                     /locus_tag="VGT87_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814154.1"
                     /translation="MNNRESKLTGFYDVFEEEVEKINHIHNVFVEESEKFGFQQMKIA
                     MVEHRKKYLDATDVHYTKYKNI"
     gene            10572..11057
                     /locus_tag="VGT87_001307"
     CDS             10572..11057
                     /locus_tag="VGT87_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814155.1"
                     /translation="MLFRDRIPTYKGYRRQFNQILLGAWGIESAYVDAEIIVATWRGL
                     QRFKGIKVEFIQLSNKNIFDVLEKDLSKKLRFEDISIEAILGKYLCNNDIEIIKCLYE
                     KDKINMELLISLISKISNKLVKQEFIKVLVLYEYVKTFYQQIAFTFHYLIYMEQDIIQ
                     V"
     gene            11054..11470
                     /locus_tag="VGT87_001308"
     CDS             11054..11470
                     /locus_tag="VGT87_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814156.1"
                     /translation="MNYKIFIRTKSGDIFDIADGGRIDDMVSKFNKVNVLGVCMGIGT
                     TVLSQEIEYEIEDRIMILVEKIDVKIYKNCLELANKISGYHCSVFEFPYKKIKKFFKH
                     ELYSRHHYIIVRLDGSMEYRFSSVALKNKIKSILDS"
BASE COUNT         3902 a         1671 c         2294 g         3882 t
ORIGIN      
        1 agatgatgct ctgagaaaat gtaattcaaa agtttttaca cgtgatattt ttcagtatga
       61 ttaacaaaaa catttttgcg aaaaaatatt tttaagtaat aaattcagtt aaaagttatt
      121 atatctgaaa attttaaagg tctttaaaat gaacaatagg aaaaaaaata ttcaatattt
      181 agtgtatagt aaagttatat atcgaattgg cgatgttatg tttgattttg ctaacaatac
      241 ttttcttgct gggcttaatc cagcgtctct atcattagtt gcagtatatc agtttctaga
      301 aagtgtcata ggtgttctat ttaatttatt tggtggagtc attgcagaca gtttcaagcg
      361 gaaaaaaatt attattacta caaatatctt atgtggcaca gcttgcttag tactctcgtt
      421 cctaacaaaa gagcaatggt tggtttatgc cattgtacta actaatgtta tcttggcatt
      481 tatgagtgct ttttctagtc catcctataa agcatttaca aaagaaattg ttaaaaaaga
      541 tagtatatca caacttaatt cattgctaga gacaacaagt actgtaatca aagtaacagt
      601 tcctatggta gcaattttct tatataagct acttggtata cacggtgtat tactattgga
      661 tggactatca tttctaatag ctgctttact aatttccttt attttacctg ttaatgacga
      721 agtggtgata aaggagaagg tgacaatcag agaaatattt aacgatttaa aaataggatt
      781 taaatatgtt tacagccata agtccatctt tattattacc gttctttctg cacttgttaa
      841 tttttttcta gccgcttata atttattgtt gccctatagt aatcaaatgt ttggagaaat
      901 ttcagctgga ctttatggca cttttttaac agcagaagca attggtggat ttattggggc
      961 aatattaagt ggttttgtga ataaagaatt atcaagtatg agattgatat tgtttctatc
     1021 tttatcagga ctgatgttaa tgttagctcc cccattttat attatgtttc ataatgcaat
     1081 tattttagct ttgtcaccag ctttattcag tttatttctt tccatcttta atattcaatt
     1141 tttttctctt gtccagaaag atgttgataa cgatttttta gggagagtgt ttggtattat
     1201 ctttacaata actattcttt ttatgcctat tgggactggc tttttctcgg tggcattaaa
     1261 tcctaacaat agctttaatc tttttattat tggttcgtgc attactatat tgtcattagt
     1321 atttagaata ttatttaaaa agtataatat tcgttaacag ttagtactaa gtattattga
     1381 gaaaattatt tttttcaaag taaaggcact aagttgatct gacttacaca tcttatcaac
     1441 tcagtgcttt ttttatttct agatattatt atctacaatt tctactatat cttcaatctt
     1501 acaatcaagt gcaatacaaa tttccaataa aatatcagta gtgatatttt ctccttttcc
     1561 caattttgcg atcgtggaag aactaatccc agcttcttgc ctgagctttg ttttggtcca
     1621 acctttatca ataagaagtt tccataattt attgtaacta aatctcatct gagttttctc
     1681 ctttgttcca tgaaataccg tagagttcat ttccgttact agagagtctc aataccgtat
     1741 tttctgacaa gagttgatta gcatctggag aataagagct ggctttatcg aaggctaaaa
     1801 agacttgttt gttcaatgac tttgactggt catacagctt caaaagtgct tcagtcgctt
     1861 ggtagctaac attactcaag agtaatgaat cgtgtgcgag tgctggcaga ttagtaaggt
     1921 agagcattac taagtcgaat acaatcatcc ctttgaactt agttcctgta ccatcatcat
     1981 ctggtgtata gaaggagtag ctattgtgag cattgaggtt aattgaggag ctttgcgaat
     2041 atctggatat agaatgttat tgaattcacg catcttactg ttgattttta cttctagatc
     2101 tagaaggata tcttctgtac tgcgtttcaa gtcagcgtct gcttctgatt tttctttgga
     2161 tagaccagac tcctttaagt aggcctcgtt ttgttctttt agtgcattga ttgtagctgt
     2221 tagtttagag tagttttcta agtactcact tgaaagattt gggatgtttc ctagttcttg
     2281 aagttcttgg tttagaattg ttaattgact ctctaattca tcgatctcag ctattaggga
     2341 ttcacgttca gttgagaatt cggaatctaa aatagttgcc aattttttgt ggtaagcttc
     2401 aacttcatat aattttttga gattagtatc tgggaagtat ttttgaagct ctgttaagtc
     2461 agattcagta ggatataagc caaattcgat actgatgtcc aatagcttaa gacgtctttg
     2521 cttgtcccga agactgcttt ctaactccag cttagtattt ctaagttgga gtttttgttg
     2581 atttttctca atatcatcag agtcaatttt atcttgatga gtatcttgga gatgtgctat
     2641 atcatattcc aatcttttta tttctgagac attcgcttca aattgctttt taccgccaac
     2701 caggttggag atgaaggcat actttcgagc attacgaaaa gttgttaact ggtcgctctt
     2761 atctttcaga cgttctttgt atcgtgcaat gttatcatac aaattaaaca gttggatgag
     2821 agtagtaata gattcagaag atttttggct cgaataaact tgtaagggga aatcggtatt
     2881 ttgattactt tttcctgcaa tcctaaagaa gccactcata gctaatcgga atgatagctc
     2941 tggagaatct aaatgatatc gtttcttaag tttgttaaga aaaattccta ggtccattgt
     3001 ttcaccggtt ggagagtagc tcttgtcaca cactagaatg atatctgggt tggccgcatc
     3061 tcttgaaaaa taaaactttt tctcaaattg aaagcagaag tagataggat gatcacctaa
     3121 ttttttaacc gcaattgatt ttagatagct attacctcca aagatgaaat caatggctaa
     3181 tagtgaggag gatttgccga tagagttatc agctaaatcc atccccataa tcacgtttaa
     3241 cccatggtgg aagcagattg gttcccgaat ttctccgtgt ttcttaaata ctggagacca
     3301 catttctata agcataattt tacttctcct tcattagaca tttcaatagc gttcaatgca
     3361 tacaagcaat ccattatgga tagaaaatct gtaggatcat ccaactcttc tgagatagcg
     3421 tgaaagatat ctttgacgtt tgagtttcca cttttgattt catttagcac tctaggtaaa
     3481 taggccaggg tgcttttttg ataggagtaa agtttatttg gtaattgcat tattcaagta
     3541 cctcgcattt ttggataaag taagagacaa tcatctgaca gaagtagatg tcctgtagtg
     3601 tagctttatg gagtttttcc gaaatagtat tgaaaatagc cagattatta tgcttggctg
     3661 cttttaactt tttatacatc gatcgcgttt gatattggat ttcctcgtaa tcaatttttc
     3721 cttgtttgtc caaatttacg atgatttttt gaatggttaa gtagttatca ataactgggt
     3781 ttttaaccaa ttgataaagc gtacgattgt tctcaggtga tatcttatca gtcaattgct
     3841 tcgagtcata ggagatatca tattcatcac attttagttt attgagacta gtcaaaactg
     3901 aaacaattcc actgtctaac ttcatatcag aaatcgcttg ttggctgtta tgtgcagaga
     3961 ctagaatctt tttaacattt tttagagctg ttacaatttt tttgcgacta tctgcttgat
     4021 aggttaaaaa acaatctggg cagagggcta tcagattcag tggttcagcg tccttatctt
     4081 tttcaatggc tgaaacctcg taattttctg ctgctaagtt gccactggtt agaatgagtg
     4141 gtctatcaca gcctgggcat gcacaacagt tattagattc tgttaacaag aactgcccgt
     4201 attgtttttt gagttgattc gactgggtag tgaacgctgt attttgcaca gtggttaact
     4261 ttacaatccc tgtcttctct ctgatggtta ccagaagata ccttggaatt gcggtgtcga
     4321 cttgaccatg gtgccttttg ggcagggtta taaggatatg agtccaccgt ccaagggact
     4381 ctataaactc atgatggaag ggaaaatcta acatggtggt catccagtcg ttaaatggat
     4441 gggacaaaac gtggtgatgc gacaagctcc tgcaggcaac atcaagccag ataaggaaaa
     4501 atctgttgag aagattgacg gtattgtaag tcttatcctg gggcttgacc gttgcattcg
     4561 ccgccaaggt gatgaagcta gcgtctatga tgagcgaggg atattgagtt tttagttgag
     4621 tcaactccaa aaatgtgata caatgtcgtt acaaaaaagg aggataacca tggctaaaac
     4681 gggaactttg aatttacgag ttgatgattc agtaaaaagt gcggcagatg atattttgaa
     4741 acgcttaggt attcctatgt caactgcgat tgatatgttt ttgaatcaga ttattttgac
     4801 tgggggcatc ccatttgatg tttctctacc tgaagcgcct caacgagtta atgttgatta
     4861 catgagtcag gagaagtttt atgataagct tatcaccagc tttgaagatg cgaaaactgg
     4921 caaccgtcag gatgtcagag aatttctatt tcaattgaag gaaaatgctt aatgaaagaa
     4981 tatcaggtca ccatgtcgga tgatgctaaa gcagatttgc tgagcatcta tcactatgtt
     5041 cgtgatgaac tctgtgcacc acaggcagcg gataatcttc ttgagaggtt atcccaagga
     5101 atgttatcac tatccatttt tcctgaacgt tgtgccatca ttgaggatct gattggaaaa
     5161 ggctatacct tcagacaact gattgtcaaa aaatatcgca ttgtatatca tgttctggaa
     5221 gatgaagtta taattgttgc ggtcgtctat ggatcacgtc atatgaataa ttggtaaagt
     5281 aagggagagt gatgtatgga actcgtaaag acgatacaaa ttggtgatga catctatcct
     5341 ccgatacctg acctatttgg cattcaagag aatcaggagt ttaaccttta taaatcaact
     5401 gatggaactt tggtgatgag cagatgacaa cagttgttga acaagcaagg ttagatgaga
     5461 ttgcgacttc agtattatca cgacatcttg acgcatttaa ggaattgaca gaatgagaat
     5521 actaggtttt gtaagaaaga ctgttgatta ctttgaaaga aatataccga aagcacttca
     5581 aaggaggtgc tttttttgta ccccaaaata aggagggctt atgggactac ttgatttaat
     5641 tagaaaaaaa gggctagaga taagcctcaa aacagttatg aaggtcagga cttttcctac
     5701 ctctttggtc gcacgactag tggcgagaat gtggatgagt ttaagactat gcacacgacg
     5761 gcagtctatg cctgtgtgcg tattcttgct gaagcggtgg cgtcatttcc tattcacatc
     5821 tatgaacgga cagaaaatgg gaaggagaaa aagctggacc acccactgta ctttcttctt
     5881 catgatgaac ccaatccaga gatgtcctcc tttatctttc gagaggcaat catgagccat
     5941 cttttgatat ggggaaatgc ctatgtgcag attatacgag ataaaagtgg tatggtatcg
     6001 ctactgctaa agcagaaggc gagcgttctg ataagaaagc tgctcttgat tatctggcaa
     6061 cggatgcctt agaagcacga gttgcagcac ttcaaaaagc taagattgat gagttgaccg
     6121 tctctagttc agcatggatg acaaggctta tctcccaaca gattctatca gagtatgtga
     6181 agagcctaga agttgaagct gataaggtcg ttattccagg acagaatact ccagtcttta
     6241 gttgggatag ggatgggaat ctttccattg acacaccgct cctaaaggtg agaggggaaa
     6301 gtctagcgac acaagctgac cttacaacca tctctttaac tcctggtcca aagggagaac
     6361 tctggcagac aagtgttgaa aggttggata taaaagtaga caagcacatc agcctaacct
     6421 tcaaaaatgg cgtacggatt gattgataaa aggcaagggc gtatcaattc ggcacgtcct
     6481 tttttagttt atctgtggta taatgaaaga agttatattg ggagttgaga tgcacgattc
     6541 atttggattt tgcactccct aaaaagggta aaggttagat ttcatggtta aactaatagc
     6601 aacagaaata ggaataaagt ggataataca ataaaagttc taaatagctt gaaacaaagg
     6661 gactaggagt tttaatactc tagggtaatt ttatgtagag tatttgaaaa taataacgtt
     6721 aaaaagttag taatcgttaa agaatttaaa ataaattgta atagtttatc gcttgtttta
     6781 tcgcttgaag aacgagcgat aaatttattt ttatggtata aataagaaaa aagattgtaa
     6841 ggagaaaatg atgaaatcac cttttacagt tacgaatacc atgttaaata aggtagtaga
     6901 gatttctaaa attattggta atttagaatt acaagtacag aaagatttaa aattaagaaa
     6961 agaaaatcgt attcagtcta ttcactcatc actcgctatt gaacaaaatt ctttaacagt
     7021 agaacaaata acagccatta ttgatgggaa aagagtgtta ggaaaccccc gtgagataag
     7081 agaagtaaaa aacgcttatg aagcttatga agaaatttta acgctaactc cctatgatga
     7141 atcacatttt ttaaaaatga aggaatttca acagtacatt taccggtaac aacatcgtca
     7201 atcacaagtc ctatggggtt ggggagtgat tcgctacctg taaaaatttc cttatttgat
     7261 gttgatacct atcttgcaga ttcgatgcag tttcacttag agtatatact tagacttttt
     7321 ccaaagtcag gagttcatta cttaatgcct acttttagag gagaagcagt agatcatcga
     7381 catttatcgc aattttatca tagtgagtgt gagatagagg gagatttaca cgatgtgatg
     7441 aatttagtgg atagatatat taagtttctg gttagtgaaa ttcttagaga gtgtcgagat
     7501 agtatttttg ctataacaaa agatgtaagt catttggaaa gttttattga tcgaaagata
     7561 ccaattatta cgtttgaaga ggcttgtact atattggaaa atgaaataga ttcattggaa
     7621 tataatgatg aacatcattt ttatgtgtta acagatgttg gggaaaagaa attaatggaa
     7681 cattttgagg gagtagtttg ggtaaaaaat tatccctgta aaagtgtccc tttttatcaa
     7741 aggttctcaa cagatcatct atttgcctat aatgcagatt tgttgttagg aataggcgaa
     7801 actgtaggtt gtggagaaag atgcgaaaca tatgaagaag ttgtagaaaa tatgaattat
     7861 ttacaagtag atgcttctga atatagttgg tatttagaaa tgaaaaaaac aagacctatg
     7921 aaatcttcag gatttggttt aggagtagag cgtttaatag cgtggatatt gaagtgcgat
     7981 gatgttcgaa aagttcaatt aatcagtaga gaaaatggga ttgattattt accataaagg
     8041 aggaagaaaa atgaaaaaga atattttaat tgtaggtgga ggtccagaga taactatatg
     8101 ttatgattta attaacaata gtacggagta taatttcttt tcagtatcag aaattcctcc
     8161 gatgtatcca gagttgtttg tagaaacaat tgaaaccagc tttagaaatt ttggaaagac
     8221 gattgaacaa attaaagagc tcaaatattc tattgatgct gttgcttgtt gggatgaagc
     8281 tttaacgcat atagctgatg atatttcaaa agaattagga ttgaacccta tttcatcgct
     8341 agattctcaa tcattcaggt ttaaagatcg aatgagaatg gtatgtgaag ctggtggact
     8401 aaaaatgccc aaatataaaa ttatcaatca attttcagat acaaataaaa taatcaattg
     8461 gaaatatcca ttaatagtga aaccaacctc ttttttagca agtataggag tgaaaaaagt
     8521 ttataatttt tcagaattac aacaagcagt ttctcaaatg cttaatgtaa aatttccggt
     8581 ttacatagct tcaggggtat atgaattggg gaattatata atttagaacc tagagttttg
     8641 gtggaagagt ttattgatgg agaagaatat agcttagaga gcgtggtaag aaatggaatc
     8701 tatacacctc tagggataac taaaaaatag tggatgaaaa attatttatg gatgaaatag
     8761 gccatatttt cccttccaat ttaaacaaag aagaaaaaag tagagtatat tcttgggcag
     8821 aaaaattaca tcaaattttg caattaaatc atattacgac tcatacagaa tttagaattg
     8881 gaagaaatgg agatattatt ttaatagaaa ttggagcaag aatcggtgga gattgtatcc
     8941 aaacttaatg aaaaacagct ttatagtcaa gggatttgat ttttataata cttatttaaa
     9001 agctagacta ggtttacaag aagagttaaa tacagacact aataagtttt caggaattgt
     9061 atttttcaga ctcaatccag agaattacgg aaaaatattt aatggagtga gttacgatgc
     9121 acctaaaact tcactaatat ttgaagaaaa gatgtataaa gaggaaggaa ctgtattgcc
     9181 aaatccgatt aattggagta atgagagagt aggatatgtt tgtttggaaa acaaggaata
     9241 cgctgtgcta gaaaaggaga tgaaagaaat attaaaatca ttgtcaatag agtaaatagt
     9301 gaaagaaaca tcaaatctat aggtatttgt tcaccctcct atccggggac atttcattat
     9361 cagaaacgat ataagagagg agtagaattt ttacaaaata aaggatttac tatagtagag
     9421 gggaatttaa caggaaaaga aggttataga ggggaaattt caggaggtgt tcaagacaga
     9481 gctgaagaat taaatcaact tattccgcgt gtggatgtca tcatgacttc cataggtggc
     9541 tacaattcta attctatatt aaagtatata gactacgatc tatttaaaca gaaatctcct
     9601 atctttattg gatattctga cacaacagct cttgctttag ccttatataa taagactgga
     9661 tgtataactt acctgtctca gagcgttatc tctaattttg gagagtttga accgtttaat
     9721 gaattaaatt atttctattt tgacttcatg cttcagagta agtgcgaaac cttaatggta
     9781 caaataccag atgtttggac ggatgagtgg ataaattggg aaacctatga aagaaccaaa
     9841 aaaacaaata aaaatgagtg gattattttt aacaaaggtg aatttaacgg tactttgatc
     9901 ggaggaaatc tcgataccat tgtaggaata ataggaactg agtatatgcc aaaaataaca
     9961 gaggatacca tactcttgtt agaagatgta tatacggatc taggaagatt atataggaat
    10021 ttcacaacgc ttgccttaca tggtattttt gataaaatag gaggacttat tatttcaaaa
    10081 tttgaaacaa taggggaaaa tagcgatgtt attaatgata tcattaacga atttgtaggg
    10141 catagaaaga ttcctatttt attgaatttt gattgtggtc atacccatcc tagctgttta
    10201 atgcctattg gtggtaaaat tacgttgagt ttaagctaaa agaggtggta tatgaacaat
    10261 agagaaagta aattaacggg tttctatgat gtttttgagg aagaagtaga aaaaataaat
    10321 catattcata atgtttttgt agaggagtca gaaaaatttg gctttcaaca gatgaagatt
    10381 gctatggtag agcatagaaa aaaatactta gatgcaaccg atgttcatta tacaaaatac
    10441 aaaaatattt gaagttcata gagtaaaaga agagtcagta tacgccctcc aatcagattt
    10501 ggcacttagt atgtcgccgt tgtatagcta acttaccaca acaaatacct gtaaagtgga
    10561 ttcaagacag agtgttattt agagatagaa ttcctacata taaaggttat agaagacaat
    10621 ttaatcaaat attattaggt gcatggggaa tagaatcagc ttatgtggat gcagaaataa
    10681 tagttgcaac ttggcgaggc ttgcaacgat ttaaaggaat taaagtagaa tttattcaac
    10741 tgtctaacaa aaatatattt gatgttttgg agaaagattt atctaaaaag ttaagatttg
    10801 aagatatttc aattgaagca atattgggta aatatttgtg taataatgac atagaaatca
    10861 taaaatgtct ttatgaaaaa gacaaaataa atatggaact cttaattagt cttatttcaa
    10921 aaataagtaa caaactggtg aaacaggaat ttataaaagt tttagttctg tacgagtatg
    10981 taaaaacttt ttaccagcag attgcattta cttttcatta tctaatttat atggaacagg
    11041 acattattca agtatgaact ataaaatctt catacgaact aagtcaggtg acatatttga
    11101 tattgctgat ggtggtagaa ttgatgatat ggtttctaaa tttaataaag taaacgtatt
    11161 aggagtttgc atgggaatag gaaccactgt tttatcacaa gagattgagt atgaaattga
    11221 agatagaata atgatattag ttgaaaaaat agatgtgaaa atctataaga attgtcttga
    11281 attagcaaat aaaataagtg ggtatcattg ttctgttttt gagtttcctt ataaaaaaat
    11341 caaaaagttt tttaaacatg aattatattc gagacatcat tatattattg ttcgattaga
    11401 tggaagtatg gaatacagat tttcatctgt agctttaaaa aataaaatta aaagcatact
    11461 tgatagttga tgtatactgt catttagaat aataatcaca tattgttgtc aacgaagccc
    11521 accatagcct ataatgaaag cctagtaggg atgagctata taccaactat ctatcaatcc
    11581 agacgtctct actaaaaaat actccgtctt ctatgaaact agaaacggag tatttctgtt
    11641 tgtctatacc actaaatgaa cagttatata gaaaggtgat tggatgtttg gaataaacat
    11701 cctgaaaaaa atttgagacc ttcttatatc ctaaactaga acgaatgtc
//
LOCUS       DAUCLR010000059        11696 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.059, whole genome shotgun sequence.
ACCESSION   DAUCLR010000059 DAUCLR010000000
VERSION     DAUCLR010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11696
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.059"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..430
                     /locus_tag="VGT87_001309"
     CDS             <1..430
                     /locus_tag="VGT87_001309"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814157.1"
                     /translation="ESGAAYVSDYEGPYYEGRNKGKEDGYSAGNHTGVPDAPGPHTSP
                     FDPYSSDKRSSYLYRSGYEEGYTQGYYEGWHNANDSVTEGQDNLEGSSDDSNDSKGLE
                     SQVDSENPMDMSKLSILDLVYGMVKDFWGYMLDVLTGNAK"
     gene            448..903
                     /locus_tag="VGT87_001310"
     CDS             448..903
                     /locus_tag="VGT87_001310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814158.1"
                     /translation="MKSNLKKITAVYLALACINIFSMRPVSADDSVHPTSGSGVVDLG
                     DNVNSARSDVESLQEVGQAQDKGYDMGEKTGYQDGLRVDAPKVPKNPRPTPSTNPYSS
                     NPEAKSTYDEAYKDGYEFGYRKGWDDGHPIQATLDWLWITITSWFSRFI"
     gene            1261..1512
                     /locus_tag="VGT87_001311"
     CDS             1261..1512
                     /locus_tag="VGT87_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814159.1"
                     /translation="MPEKKVLPVLSMVVSFVCLLDHIFFFATYSYLYLPLVASSLALI
                     GLVQNKGRQKVWSLAGLILAALAMAVVAYAYYDPTVIPY"
     gene            complement(1606..2238)
                     /locus_tag="VGT87_001312"
     CDS             complement(1606..2238)
                     /locus_tag="VGT87_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814160.1"
                     /translation="MKNTLTLLLVLFAIGVTSSVRADTYGNQRIWGFRTDWEKGRDFG
                     ARPIKKGDSDIKIKTAPEAVLTVKKWENNKWTTIPQKKNTTLSSTEAQKTFSVADGAG
                     LAVFYLGNEHIANIGDKYKVTLTIDGFYLASEEWTVGESLPKDEEERDEAEIQKFIEE
                     LEQRQRESEDQKYAEELFKLSIKEEDHKTWHQRLSESIQDQWWNFKGLFQ"
     gene            complement(2752..3720)
                     /locus_tag="VGT87_001313"
     CDS             complement(2752..3720)
                     /locus_tag="VGT87_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3644 domain-containing protein"
                     /protein_id="HEQ3814161.1"
                     /translation="MENLSERLVLKSVEAFIMGLEIYNKPTIKYRIEGFSFFICNAWE
                     LMLKAEMLNRGFSIYYSDNPERTLSLEYVLKKIYSDENTRIRLNLEKIIELRNISTHF
                     ITEDYEIKYAPLFQACVLNLASEMKRFHNKDITESIAHNFLTISESYEPLSNEEIKLK
                     YPSEIAEKFIKQANEVDVLSKEYNSEKFSIDIRQNLYITKKKDEADFVVSISKDAETP
                     VSLVKELKDPSDTHKYSYSNVITAVQERLKKSAIKLGYEKGFNTYVLNLVIDFYNIKS
                     ETKFSYCHLIGSTQTYTYSQQFVEFIVNEIKKDPQNFVESLKKSNK"
     gene            4444..4680
                     /locus_tag="VGT87_001314"
     CDS             4444..4680
                     /locus_tag="VGT87_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018364244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEQ3814162.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGGAENYVQLFDTLEVDGKVLQAT
                     PYFLIHVTGAGEGFSMWAPTPCDVLAEDWIEVND"
     gene            4673..5371
                     /locus_tag="VGT87_001315"
     CDS             4673..5371
                     /locus_tag="VGT87_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEQ3814163.1"
                     /translation="MTKVVLVTGCASGIGYAQARYFLRQGYHVYGVDKSDKPDLNGNF
                     HFIKLDLSSELSPLFKVVPSVDIVCNTAGILDAYKPLLDVSDEEVEHLFDINFFATVK
                     LTRHYLRRMVEKQSGVIINMCSIASFIAGGGGVAYTSSKHALAGFTRQLALDYAKDQI
                     HIFGIAPGAVKTAMTASDFEPGGLADWVARETPIGRWTKPDEVAELTGFLASGKARSM
                     QGEIVKIDGGWTLK"
     gene            5447..6406
                     /locus_tag="VGT87_001316"
     CDS             5447..6406
                     /locus_tag="VGT87_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEQ3814164.1"
                     /translation="MKLAVLGTGMIVKEVLPVLQKIDGIDLVAILSTVRSLKTAKDLA
                     KAYHMPLATSEYEAVLGNEEIDTVYIGLPNHLHFAYAKEALLAGKHVICEKPFTMTAG
                     ELDELVIIAKKRKLILLEAITNQYLSNMTFIKEHLDQLGDIKIVECNYSQYSSRYDAF
                     KRGDIAPAFNPKMGGGALRDLNIYNIHFVVGLFGRPKTVQYLANMDKGIDTSGMLVMD
                     YEQFKVVCIGAKDCTAEIKSTIQGNKGSLAVLGATNTLPQIELVIYGQKPQVINHNKH
                     DHRMYEEFVAFRDMIDQRDFEKVNQALEHSRAVMAVLERAVHS"
     gene            6739..8076
                     /locus_tag="VGT87_001317"
     CDS             6739..8076
                     /locus_tag="VGT87_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEQ3814165.1"
                     /translation="MEEDYNKREPEKFTQFLRRQKVVFFVAFFGYVCAYLVRNNFKLM
                     SNTIMVQNGWDKAQIATLLSCLTVSYGLAKFYMGALGDRVSLRKLFSISLGASALICI
                     LIGFFNSSMVVLGILLVLCGVVQGALAPASQAMIANYFPNKTRGGAIAGWNISQNMGS
                     ALLPLTIALLTSMGLVVPANGNILLAFLIPGVLVFLFALCCWKLGGDNPESEGLDSLR
                     TMYGDAGESAVASEEEKHNLSYWQLIWKYVFCNPSLLLVAAVNVALYFVRFGIEDWMP
                     IYLSQVANMSEAHIHFAISMLEWVAIPGSLVFAWLAVRYPNKMAKIGAIGLFVLAAIV
                     FVYERLTATGAPNYFLLLVIAGILGSLIYGPQLIVNILTINFVPLNVAGTAIGFVGVT
                     AYLIGNMGANWLMPILADGFGWFWSYIVVAALSAFSAVGYLILAKREEEIIKD"
     gene            8249..9631
                     /gene="glmU"
                     /locus_tag="VGT87_001318"
     CDS             8249..9631
                     /gene="glmU"
                     /locus_tag="VGT87_001318"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEQ3814166.1"
                     /translation="MTNYAIILAAGKGTRMTSDLPKVLHKVSGLTMLEHVFRSVKAIS
                     PEKAVTVIGHKSEKVRAVLADQSAFVHQTEQLGTGHAVMMAETQLEGLEGHTLVIAGD
                     TPLITGESLKSLIDFHVNHKNVATILTATAQDPFGYGRIVRNKDGEVIKIVEQKDANE
                     YEQQLKEINTGTYVFDNKRLFEALKCITTNNAQGEYYLTDVVAIFRANKEKVGAYILR
                     DFNESLGVNDRVALATAETVMRQRITQKHMVNGVTFQNPETVYIESDVEIAPDVLIEG
                     NVTLKGRTHIGSGTVLTNGTYIVDSEIGQGSIITNSMIESSVLAAGVTVGPYAHLRPG
                     TTLDREVHIGNFVEVKGSHIGEKTKAGHLTYIGNAQVGSSVNVGAGTITVNYDGQNKY
                     ETVIGDHAFIGSNSTLIAPLEVGDNALTAAGSTISKTVPADSIVIGRSRQVTKEGYAK
                     RLAHHPSRSK"
     gene            9662..10216
                     /locus_tag="VGT87_001319"
     CDS             9662..10216
                     /locus_tag="VGT87_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEQ3814167.1"
                     /translation="MKFEEKTLKRQTVFDGHIFKVVVDDVELPNNLGQSKRELIFHRG
                     AVAVLAITPERKIVLVKQYRKAIESVSYEIPAGKLEIGEEGSKLKAAARELEEETAYT
                     GTLTFLYEFYTAIGFCNEKITLFLATDLTQVANPKPQDDDEVIEVLELTYQECMDLVA
                     QGKLADAKTLIALQYYALHFGGEQ"
     gene            10216..10467
                     /locus_tag="VGT87_001320"
     CDS             10216..10467
                     /locus_tag="VGT87_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814168.1"
                     /translation="MGRPLLTDDIIEKARRMETFEPDDAVDFDTKVMTLPEKDEKARI
                     YKSRRIENAKRSQLQSKLNVILIAVMLLIAILVYAIFYL"
     gene            10486..11181
                     /locus_tag="VGT87_001321"
     CDS             10486..11181
                     /locus_tag="VGT87_001321"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEQ3814169.1"
                     /translation="MKIGIIAAMEEELSLLLANLLDAQEHQVLSKTYYTGRFGKHELI
                     LVQSGVGKVMSAMTVAILVEHFKAQAIINTGSAGAVASHLAIGDVVVADRLVYHDVDA
                     TAFGYAYGQMAGQPLYYDCDPQFVAIFKQVLKHEKTNGQVGLIATGDSFVAGQDKIDQ
                     IKTAFSDVLAVEMEGAAIAQAAHTAGKPFIVVRAMSDTAAHDANITFDQFIIEAGKRS
                     AQILMTFLENSPV"
     gene            11332..11673
                     /locus_tag="VGT87_001322"
     CDS             11332..11673
                     /locus_tag="VGT87_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814170.1"
                     /translation="MTLKKHYYLLSLLALVTVGAAFNTSQSVSAQVYSNGGYRQGLTD
                     EKSHLQYSKDNAKLQLRNILNGYQSDLGRNYSSYYYYNLRTVMGLSSEQEIEKYYEEL
                     KNKLHDMYNHY"
BASE COUNT         3466 a         2061 c         2515 g         3654 t
ORIGIN      
        1 cgaatcagga gctgcttatg taagtgatta tgaaggtcca tattatgagg gtaggaacaa
       61 gggtaaagaa gacggttata gtgctggcaa tcacacaggt gtccctgatg ctcctggacc
      121 acatacttct ccttttgacc cctatagctc tgataaacga agcagctatc tatatagatc
      181 tggttatgag gaaggatata ctcagggata ttatgaggga tggcataacg ccaatgattc
      241 tgttactgag ggtcaggata atctagaagg ttcttctgat gactcaaacg actcaaaagg
      301 gttagaatct caagtagata gtgaaaatcc catggatatg tcaaaacttt ctattttaga
      361 tctagtttat ggaatggtta aagatttttg gggatatatg ttagatgtac tcacaggaaa
      421 tgctaaataa ggaaataggt taatttaatg aaatcaaatt taaagaaaat cactgctgtt
      481 tatttagctt tagcatgtat aaatatcttt tctatgagac cagtatcggc tgatgattcc
      541 gttcatccta cttcaggatc aggagttgtt gacttaggtg ataatgtaaa ttcagctagg
      601 tcggatgtag aatcattgca agaagttgga caagcacaag ataaaggtta tgatatgggt
      661 gaaaaaactg gttatcagga tggattacgg gtagatgctc ctaaagtacc gaaaaatccg
      721 agacctacac cctctactaa tccttatagt agtaatccag aggcgaaatc tacgtacgat
      781 gaggcgtata aagacggata tgagtttgga tatcgtaagg ggtgggatga tggccatcca
      841 atacaagcaa ctttagactg gttatggata acgataactt cttggttctc acgttttatt
      901 taatctaagc aagatgctct tgaaatggat gagcaaaatc tggataaaaa agctaaggag
      961 acggctatag tgatggttat caattaggat actatcaggg atggcgtgaa acaaatcatc
     1021 cgatacaagc ggcattagag tctgcactag actggctatg ggatttccta tcatcattct
     1081 ttccagctga cagtgagcaa aattaattat agtaaacaat aaaaattatt tggttaacat
     1141 cacgctattt taaaacaatt ttctgctata attgggatag tccctttggt agtcaggcca
     1201 cttctgatag gggtggcttt tttgggtttt agtgatccgt gtgttgagaa aggagctttt
     1261 atgccagaaa agaaagtatt gcccgtccta agcatggtgg tgagttttgt ctgcctgctt
     1321 gatcatatct tcttttttgc tacttatagt tatctctact tgccgcttgt agcaagtagt
     1381 ctagctctta tcggtctggt gcaaaacaaa ggcaggcaaa aggtttggtc gctagctggg
     1441 cttatcttag ctgctctagc tatggcagta gtggcctatg cctactatga cccaaccgtc
     1501 atcccctact aaccttcccc cctctttgca cgcaatcaac atcacaaaaa gcacccctga
     1561 caggtgcttt ttgtacttta gctaacctca tagcctagca aaccctcact gaaacagtcc
     1621 cttaaagttc caccactgat cttggatgct ctcacttaac cgctgatgcc aagttttatg
     1681 gtcttcttct tttatagaaa gtttgaatag ttcctcagcg tatttttggt cttctgattc
     1741 tcgttgtctt tgctctaact cttcaataaa tttttgaatt tcagcttcgt ccctttcttc
     1801 ttcatctttt gggagcgatt cccctacggt ccactcttca ctagctaagt aaaaaccatc
     1861 aatagtgagt gttactttgt atttgtcgcc tatatttgct atatgctcat tccctagata
     1921 aaacactgct aaacctgcac cgtcagcaac agaaaaagtt ttctgtgctt cagtactact
     1981 taaagtggtg ttcttttttt gtggaatagt tgtccattta ttattttccc acttttttac
     2041 agttaagact gcttctggtg ctgttttaat tttaatatca ctatcccctt ttttaatagg
     2101 gcgagcacca aaatctcgtc ctttttccca atctgttcta aatccccata ttctttgatt
     2161 gccatacgta tccgctctga cgctactagt taccccgatg gcaaagagta ccagtagcaa
     2221 ggttaatgta tttttcattt ttttatcccc ttttgctcat tttttaagac aaggcttatt
     2281 ataccaattc ttgcgctaaa aagataggga gtttatgtct tgtggttggg aaatggcgcg
     2341 actattcttg gtaggggcag acagactgac taaagttcct attagcgcaa cggatatcgt
     2401 ccataaagaa gaacaatatg acatttgaag agattctcga tcctagatag ttactgcatt
     2461 tcatagggtt tcttatctag tagcattgag gattttatca cacaaatcgt ttcatagacc
     2521 tgttttaaag gccatcacaa gtcccactaa aaaggggata aaaaaaggcg tccacaggca
     2581 agaaaaaagg caccttacgg ggtgtctttt aattaatttg tcaataataa ttgacgttta
     2641 ttttgtaagt ggtataatat agttaataaa aaaactcgtg aaggataaac gctgggtccc
     2701 aaaatggggt aggtacttag tactgagcat tcctatgtgc ctggggtttt tttatttgtt
     2761 cgatttttta aggctctcaa caaagttttg agggtctttt tttatttcat ttactataaa
     2821 ttcaacaaac tgttgtgaat atgtataagt ctgtgttgat cctataagat gacaatatga
     2881 aaattttgtt tcactcttaa tattatagaa atcaatcact aaatttaata cataagtatt
     2941 gaaccctttt tcatagccaa gttttatcgc agactttttt agcctttcct gaactgctgt
     3001 tattacattg ctataggaat atttatgagt atctgacgga tcttttaatt ctttaactag
     3061 tgaaacaggg gtttcagcat cttttgaaat actaacaacg aagtcagcct catctttttt
     3121 cttagtaata taaagatttt gtctaatatc aattgaaaat ttttcagaat tatactcttt
     3181 actcagtaca tcaacttcat tagcttgttt gatgaatttt tctgcaattt ctgaaggata
     3241 ttttagcttt atttcctcat tggataaagg ttcgtaactt tctgatattg ttaaaaaatt
     3301 atgtgctatt gattcggtaa tatctttatt atggaatctt ttcatttctg acgctaaatt
     3361 taaaacgcat gcttgaaata atggagcata tttaatttca tagtcttcag taataaagtg
     3421 ggtgctaata tttcttaatt caattatttt ttcaagattt aaccgaattc tggtattttc
     3481 atcagaatat atctttttta aaacatattc taggctcaat gttctttctg gattatcact
     3541 gtaataaata ctaaaccctc ggttaagcat ctcagctttt agcattagtt cccaagcatt
     3601 acatataaaa aaactaaagc cttcaattcg atattttatt gtcggtttat tatatatttc
     3661 gagccccata ataaaagctt cgacactttt taaaactaat ctttcactta aattttccat
     3721 ttaattactc cgtttatttt attatataca ttatatcatc agtattaagt gttttataga
     3781 atttcagaaa gattttatag aattattatt acggtttttc gattttggaa aataaaaggt
     3841 accttcaagg tgacttttat tatttaagtg gcttaatgaa ttggttaaaa tctttttagt
     3901 ttagtaaaat tgctatttaa ggttagccac ctctcgtatg gtcacagttg atgaattatg
     3961 ctatagtata gacataaaaa atagttcaaa ttccttcttg cctgttggtg gattttgaac
     4021 tgaaagcctc tctgattgca gttggtaggc ttttttgatg ctttttcgta ctttttttat
     4081 ttattacgct aaaaaatgtt atacttgatg aagaaaaagg agagtttaga acgttaagat
     4141 acctcacagc agattgatag aggatatgga agaaggttag ctttcccaag agatgccgtt
     4201 tgggaatggt ataacaataa ctggcagaag ttataagtaa aacaaaaaca aattaactca
     4261 gatgattttt agacgttccc acgcgtgtgc gaatgcgtgt gcgaattgat gttatcaata
     4321 aatcctttaa taacgccttt ttcgaacagg aaacaaattt attatcccac gcgtgtgcga
     4381 atgtgtgggc gaattaattg gagaaaaaat gagaaaatac gatatacaaa aaaggagaac
     4441 aatatgacat ttgaagagat tttaccaggt ttaaaagcca agaaaaaata tgtacgtaca
     4501 ggttggggtg gcgctgaaaa ttatgtgcag ttgtttgata cccttgaagt ggatgggaaa
     4561 gtactacagg cgacgcctta ttttctgatt catgttactg gtgcaggtga aggtttttcc
     4621 atgtgggcac cgacaccatg tgatgtgtta gcagaggatt ggatcgaggt caatgactaa
     4681 agttgtttta gtgacgggct gtgctagtgg gattggttat gcacaggccc gctatttttt
     4741 gagacaaggg tatcacgtat atggtgtgga taaatcggat aaaccggact taaacggtaa
     4801 ttttcacttt atcaaattgg atttatcctc tgagttatcc ccactattta aagtggtccc
     4861 cagtgttgat attgtgtgca atactgcagg gattttagat gcctacaagc cacttttaga
     4921 cgtcagtgat gaagaagtgg aacacctatt tgatattaat ttttttgcga cggtgaagtt
     4981 aacacgtcat tatcttagac gtatggtgga aaagcagtca ggtgttatca tcaatatgtg
     5041 ctcaatagca agttttatag cgggtggcgg tggtgttgcc tatacgagtt ctaagcatgc
     5101 ccttgcggga ttcacgcgcc agctggcttt ggattacgcc aaagatcaga ttcacatttt
     5161 tggtatagca ccaggtgctg tcaaaaccgc tatgacggct agcgattttg aacctggtgg
     5221 cttagcagac tgggtagctc gtgagacgcc aattgggcgt tggactaaac cagatgaagt
     5281 tgctgaatta acgggatttc ttgcgtcagg aaaagcgaga tcaatgcaag gtgaaattgt
     5341 caaaattgac ggtggctgga ctttgaaatg atcacatgcc ttagcttgag ctggcgcagt
     5401 aagccctaga ggtgtgacga gtcaaacagt caaaggagat gaacaaatga aattagcggt
     5461 tttggggaca ggtatgattg ttaaggaagt tttgcctgtc ttgcagaaaa ttgatggtat
     5521 tgatctagtt gctatcttat caactgttag aagtttaaag acagctaaag acctcgctaa
     5581 ggcatatcac atgcctctag ctacgagtga atatgaggct gtcttaggca atgaggagat
     5641 tgacacggtc tatattggac tgccaaatca cctgcatttt gcctatgcca aggaggctct
     5701 tctggctggt aaacatgtca tttgtgaaaa gccctttacc atgacagcag gagaattaga
     5761 tgaactggtg ataatcgcta aaaaacgaaa actcattttg ttagaagcga ttacgaatca
     5821 gtatttaagc aacatgacct ttatcaaaga gcatcttgac caacttggcg atatcaaaat
     5881 tgtagagtgt aattattcac agtattcttc acgctacgat gccttcaaac gaggtgatat
     5941 cgcaccagcc ttcaatccta aaatgggtgg tggagcttta cgtgatttaa acatttacaa
     6001 cattcatttt gtggtaggat tatttggtcg ccctaaaacg gttcaatatt tagccaatat
     6061 ggacaagggc attgacacct caggcatgct tgtcatggac tatgagcagt ttaaagtggt
     6121 gtgtatcggg gccaaagact gcactgcgga aatcaaatca accatccaag gtaataaagg
     6181 gtcactggca gtgcttggag caacaaatac ccttcctcag attgagctcg ttatttatgg
     6241 tcaaaaacca caagtgatta accacaacaa gcatgaccac cggatgtatg aagagtttgt
     6301 cgcttttagg gacatgattg accaaagaga ctttgagaaa gtgaatcagg ctttggaaca
     6361 tagccgagct gttatggctg tgttggagcg ggctgttcat tcgtgattgt aatctggtag
     6421 ccattgcctt ttgtgcagct gctatggtaa tggttgaaag acgtgttttc acgagggtta
     6481 accccctcgt gcttttttgg taaaaataac ttgttcagga tttgggcttt ctttgtgaga
     6541 tgagagatgc ctgacgaatt gatttgatta aaataatgat aaagccaaac gatttaagaa
     6601 atgaccgttc ggaaattctg tcccaaagga gctctagaag cagtaaaatc gaatttgtaa
     6661 gcgttgacac tgttgcataa ctctgttaca ctaagagaat gtcatgacaa aaattacagt
     6721 gtaggagttt aattgtttat ggaagaggat tataacaaaa gagaacctga gaagtttacg
     6781 caatttctgc gccgtcaaaa ggttgtcttt tttgtcgcat tttttggtta tgtgtgtgct
     6841 tacttggtac gcaacaattt caaactaatg tcaaacacta ttatggtcca aaacggttgg
     6901 gacaaggctc aaattgcgac cttgttatcg tgtttgaccg tttcttatgg cttagcaaaa
     6961 ttctatatgg gggccttagg ggatcgtgtt agcttgagaa agctcttttc gatcagcttg
     7021 ggtgccagcg ctttaatttg tattttaatc ggttttttca atagttcaat ggtggtatta
     7081 ggaatactgc tagtcttgtg tggtgtcgta caaggtgcct tggcacctgc ctcacaagcc
     7141 atgattgcca attattttcc aaataaaaca cgcggcggag ccatcgctgg atggaacatt
     7201 tctcaaaaca tgggatcagc cctcttgcca ttaaccatcg ccttgctcac cagtatgggc
     7261 ttggtggtac cagctaatgg caatatttta cttgcctttt taatccctgg tgttttagtg
     7321 ttcttgtttg ctttgtgttg ctggaagctt ggcggtgata accctgagtc tgagggactt
     7381 gactctcttc gaacgatgta tggtgatgcg ggcgagtcag ctgttgctag tgaagaagaa
     7441 aagcataacc tgtcttattg gcaactcatc tggaaatacg ttttttgtaa cccgtcgctc
     7501 ttacttgttg ctgctgtcaa tgtggccctt tattttgttc gttttgggat tgaagactgg
     7561 atgccgatct acttgtcaca agtagccaat atgtcagagg ctcatattca ttttgcgatt
     7621 tcaatgttag agtgggtagc tattccaggc tcgctggtat ttgcgtggtt agcggttcgt
     7681 tatcctaata aaatggccaa gattggggct attgggcttt ttgtgttagc ggctattgtc
     7741 tttgtctatg aacgcttgac tgccacaggt gctccaaact atttcttgtt gcttgttatt
     7801 gcaggtattt tagggtcatt gatttatggc ccacagttga tcgtgaatat tttaacaatt
     7861 aactttgttc ctttaaatgt tgcaggaaca gcgattggtt ttgtaggagt aacagcttat
     7921 ctcatcggca atatgggagc aaactggctg atgccgattt tggcagatgg ttttggctgg
     7981 ttttggtcat atattgtcgt tgcagcctta tctgcttttt cagcggttgg ttatttgatt
     8041 ttagccaaac gtgaggaaga aatcatcaaa gattagctag gcaaatgcct aagaaagaca
     8101 gacttattgc tgaagtcctg tcttttttag tcatcacaag ccgcaaatga cactataaaa
     8161 gacaagaaaa tgctaggatg tttcaggaaa aaaagagaaa atatgctata atgagaaggt
     8221 atttaaatta tatagtaagg aaaaacaaat gacaaattat gccattattt tagcagcggg
     8281 caaagggact cgcatgactt ctgatctccc aaaagttctt cataaagtat ctggactaac
     8341 catgcttgag catgtttttc gtagtgtaaa agcgattagt cctgaaaaag ccgtgacggt
     8401 tattggtcac aaatcagaaa aggtacgtgc tgtcttagca gatcaatcag cctttgtcca
     8461 tcaaacagag cagttaggaa ctggccatgc cgtgatgatg gcagaaacac aactagaagg
     8521 actagaaggg cataccttgg tgattgcagg agatactccc ttgatcactg gagaaagcct
     8581 gaaaagtctg attgattttc atgtgaatca taaaaatgtc gccactattt taacagctac
     8641 ggctcaagat ccatttggtt atggtcgtat tgttcgcaac aaagatggcg aagtgatcaa
     8701 gattgttgag caaaaagacg ccaatgagta tgagcaacaa ttaaaagaaa tcaatacagg
     8761 aacctatgtg tttgataaca agcgtctctt tgaagcgctc aaatgcatca ctaccaacaa
     8821 tgctcaagga gagtattacc tgactgatgt tgtggctatt tttagagcaa acaaagaaaa
     8881 ggtaggagcc tatatcctga gagacttcaa tgaaagtctt ggtgttaatg atcgtgtggc
     8941 cttagcaaca gctgaaacag tgatgcgcca acgcatcacc caaaaacata tggtcaatgg
     9001 ggtgactttc caaaatcctg aaaccgttta tattgaaagc gatgttgaga ttgctccaga
     9061 cgtccttatc gaagggaatg tgaccttgaa agggcgtaca catatcggct caggaactgt
     9121 cttgacgaat ggtacttata ttgtggattc tgaaattggt caaggaagta tcatcaccaa
     9181 ctcaatgatc gaatcatctg ttttagctgc aggagttacg gtaggccctt acgcccacct
     9241 tcgaccagga acgaccttag atagggaagt ccatatcggt aactttgttg aggtcaaggg
     9301 ttctcacatt ggtgaaaaaa caaaggctgg gcatctgact tatattggaa acgctcaggt
     9361 tgggtcttca gtcaatgttg gggctggaac catcactgtc aactacgacg gccaaaacaa
     9421 atatgaaaca gtcattggag atcacgcttt tattgggagc aactcgactc tcattgcacc
     9481 tttggaagtt ggagataatg ctttaactgc agctggctca acgatttcta agacagtacc
     9541 agctgatagc attgttattg gtcgtagccg ccaagtcaca aaagaaggtt atgccaagcg
     9601 cctagcgcat cacccaagtc gaagcaaata attcaagatc tatttagtaa ggaggccagg
     9661 catgaaattt gaagaaaaaa cgcttaagag acaaacagtt tttgatggtc atatttttaa
     9721 agttgttgtg gatgatgttg aacttcccaa caatctggga caatccaagc gagaattaat
     9781 ttttcatcgt ggtgccgttg ctgttttggc tattactcct gaacgtaaga ttgtccttgt
     9841 caaacaatac cgtaaggcta ttgaaagtgt ctcatacgag atcccagcgg gtaagctaga
     9901 gattggtgag gaaggctcaa agctaaaagc tgctgcacgt gagctagaag aagaaacagc
     9961 ctatacgggg acattgacgt ttttgtatga attttataca gcgattggtt tttgcaacga
    10021 aaaaatcaca cttttcttag cgacagacct aacacaagtt gccaatccca aacctcaaga
    10081 cgacgatgaa gttatcgagg tcttagagtt gacatatcaa gagtgcatgg acttagtcgc
    10141 tcaaggtaaa cttgctgatg ctaaaacgtt gattgctttg caatactatg ccttacattt
    10201 tggaggtgag cagtgatggg aaggccattg ttgaccgatg atatcatcga aaaagcgaga
    10261 cgaatggaaa catttgagcc agatgatgct gtagactttg acacaaaagt aatgacgcta
    10321 ccagaaaaag atgaaaaagc acgcatttat aaaagccgtc ggattgaaaa tgccaaacgc
    10381 agccaattgc aatctaaatt aaatgtgatt ttgatagctg ttatgctgtt aattgccatc
    10441 ttggtatatg ctatctttta tctttaaaaa acgtaaggaa atgatatgaa aataggaatt
    10501 attgctgcta tggaggagga gttgtctctg ctcttagcaa atctcttgga tgctcaagaa
    10561 catcaggtgc tttcaaaaac ttactatacg ggtcgttttg gcaagcatga gctcattttg
    10621 gtacagtcag gtgttggaaa agtgatgtct gctatgacag ttgcgatctt ggttgagcat
    10681 ttcaaagccc aagctattat caatacagga tcagcagggg cagtcgcttc acatttagcg
    10741 attggagatg tggtggttgc agatcggtta gtgtatcatg atgtggatgc tactgcattt
    10801 ggttatgctt atggtcaaat ggctggtcaa ccattatact atgattgtga tccacagttt
    10861 gtggctatct tcaaacaggt cctaaaacac gaaaagacaa atggccaagt tggtttgatt
    10921 gcaacaggag acagttttgt tgctggtcag gacaagattg accagatcaa aacagctttt
    10981 agcgacgtgt tagcagtaga aatggaaggc gcagctattg ctcaagcagc acatactgca
    11041 gggaaaccct tcatcgtcgt tcgtgcgatg agtgataccg cagcgcatga tgccaatatt
    11101 acttttgatc aatttattat agaagctggc aagcgttcgg cccagatatt aatgacattt
    11161 ttagaaaatt cacctgttta actcacctta tcgtgagtca attagctgga taatctctat
    11221 tttaggagtc tggtcacttc ttgttttgaa acctaaaaat aagaattgca atcacttgac
    11281 caaacgctat aatgggttaa ggaacgatag attactaaaa ggagtctaaa aatgacacta
    11341 aaaaaacact attatcttct cagtctgcta gctcttgtaa cggttggtgc tgcctttaac
    11401 acaagccaga gtgtcagtgc acaagtttat agcaatggag gatatcgtca aggtttgact
    11461 gatgaaaaat cacacctgca atatagtaaa gacaacgcca aacttcaatt gagaaatatc
    11521 cttaacggct accaaagtga cctagggcga aactactcta gctattatta ctacaatcta
    11581 agaaccgtta tgggactatc aagtgagcaa gagattgaaa aatattatga agagcttaag
    11641 aacaagttac atgatatgta caatcattat taagtcaaag gtttaaaaat ggcgac
//
LOCUS       DAUCLR010000060        11431 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.060, whole genome shotgun sequence.
ACCESSION   DAUCLR010000060 DAUCLR010000000
VERSION     DAUCLR010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11431
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.060"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(58..366)
                     /gene="rpsJ"
                     /locus_tag="VGT87_001323"
     CDS             complement(58..366)
                     /gene="rpsJ"
                     /locus_tag="VGT87_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEQ3814171.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAEKIVETATRTGATVAGPVPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLVDIINPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(565..1854)
                     /locus_tag="VGT87_001324"
     CDS             complement(565..1854)
                     /locus_tag="VGT87_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEQ3814172.1"
                     /translation="MIYNRRKIFSLALPSMIENILQMLMGMVDNYLVAQIGLVAVSGV
                     SIANNIISIYQSLFIALGAAVSSLIARSIGENNQNKQLNYMAGVLQVTLLLSVGLGLL
                     SVVGHHQVLEWLGAEASVTLVGGQYLSIVGGMIVSLGLLTSLGAIVRAQGYPKIPMQV
                     SLLINVLNAIFSALSIYVWGFGLLGVAWATVLSRLVGMFLLCQFIPIKQVVKRLMRPL
                     DKIIFDLSLPAAGERLMMRAGDVLIIGIVVRFGTTALAGNAIGETLTQFNYMPGLAMA
                     TATIILVARQLGGGKVTEIRYIIREAFILSTLMMLVMGALTYLLGPSLLPLFTQNTDA
                     QRSAMVVLLFSLLGAPATAGTLVYTAVWQGLGKAKFPFYATTIGMWVIRIGLGYVIGV
                     VWQYGLIGVWMATVLDNTSRWFILSKQFKKYQEITSH"
     gene            complement(2242..3258)
                     /gene="adhP"
                     /locus_tag="VGT87_001325"
     CDS             complement(2242..3258)
                     /gene="adhP"
                     /locus_tag="VGT87_001325"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEQ3814173.1"
                     /translation="MKAVVVNQASTGVEVVEHDLPNVGHGEALVKVEYCGVCHTDLHV
                     AHGDFGQVPGRILGHEGIGIVEEIGEGVTSLKVGDRVSIAWFFEGCGHCEYCTTGRET
                     LCRSVKNAGYSVDGGMSEYAVVTADYAVKVPEGLDPAQASSITCAGVTTYKAIKEAGA
                     APGQWIVIFGAGGLGNLAVQYAKKVFNAHVVAVDINNDKLELAKEVGADILVNGKEIE
                     DVPGYIQEKTGGAHGVVVTAVSKVAFNQAIDSVRAGGTVVAVGLPSEYMELSIVKTVL
                     DGIKVVGSLVGTRKDLEEAFAFGAEGLVVPVVEKVPVDTAPEVFDEMERGLIQGRKVL
                     DFIS"
     gene            complement(3510..6152)
                     /gene="adhE"
                     /locus_tag="VGT87_001326"
                     /gene_synonym="adhC"
     CDS             complement(3510..6152)
                     /gene="adhE"
                     /locus_tag="VGT87_001326"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEQ3814174.1"
                     /translation="MTEGHNTVETTSVSVTIDALVQKGLAALEEMRKLDQEQVDYIVA
                     KASVAALDAHGELAKHAYEETGRGVFEDKATKNLFACEHVVNNMRHQKTVGIIEEDDV
                     TGLTLIAEPVGVICGITPTTNPTSTAIFKSLISLKTRNPIIFAFHPSAQESSAHAARI
                     VRDAAIAAGAPENCVQWVETPSLEATNALMNHDGIATILATGGNAMVKAAYSCGKPAL
                     GVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDDFVAEFKS
                     YHTYFVNKKEKALLEEFCFGAKANSKNCAGAKLNPNIVGKPATWIAEQAGFTVPEGTN
                     ILAAECKEVSENEPLTREKLSPVIAVLKSESREDGVEKARQMVEFNGLGHSAAIHTAD
                     AELAKEFGTRIRAIRVIWNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNAVGDNVSAIN
                     LLNIKKVGRRRNNMQWFKVPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGFLDR
                     IIEQLDLRRNKVVYQIFAEVEPDPDITTVMKGTELMRTFKPDTIIALGGGSPMDAAKV
                     MWLFYEQPEVDFHDLVQKFMDIRKRAFKFPELGKKTKFVAIPTTSGTGSEVTPFAVIS
                     DKANNRKYPIADYSLTPTVAIVDPALVLTVPGFIAADTGMDVLTHATEAYVSQMANDF
                     TDGLALQAIKIVFDNLEKSVKTADFEAREKMHNASTMAGMAFANAFLGISHSMAHKIG
                     AQFHTVHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIAKLLGLPASTP
                     EEAVESYAKAVYDLGCRVGIQMNFKAQGIDENEWKEHSRELAYLAYEDQCSPANPRLP
                     MVDHMQEIIEDAYYGYAERPGRRK"
     gene            complement(6461..8236)
                     /locus_tag="VGT87_001327"
     CDS             complement(6461..8236)
                     /locus_tag="VGT87_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEQ3814175.1"
                     /translation="MRIKWFSFVRVTGLLLVLLYHFFKNVFPGGFIGVDIFFTFSGYL
                     ITALLIDEYTKKESIDIIGFLKRRFYRIVPPLVLMILLTIPFTFLIKKDFIANIGSQI
                     TAVLGFTTNIYEILTGSSYESQFIPHLFVHTWSLAIEVHFYLFWGVFVWLLARRKETQ
                     KQLRGLLFLISLGIFAISFLSMFIRSFMTSNFSLIYFSSLSHSFPFFLGAMFATITGI
                     NETTVRFQKNVRLWPRQYVLAAMVGAFTLLLVLTVTLDFNHITTYLFGFALASLFASI
                     MIYAARVLHEQTPDVQEPKAITYIADISYGIYLFHWPFYIIFSQLMSHILAVILTVFF
                     SILFATVSYYIVEPLVQGRKPNLLGLEIDCSPYYKWIVGGTLALALLTLGTCMIAPKV
                     GKFEKQLLVSSLQQAQSNMERTHTLAAGDANALSDVGIIGDSVALRSSAAFSKIMPQA
                     QLDAAVSRNFKEAFDLFNNQIKSKSLSKTVVLAVGVNSLDNYSQAVQSFIEALPNGHR
                     LVLVSPYNAKNASQVAEARDYGLKLSKKYKYVTIADWYKVAVEHPDIWYGSDGVHYSE
                     DSQGAELYVSTIQTAVEKSAKKPAK"
     gene            complement(8233..8634)
                     /locus_tag="VGT87_001328"
     CDS             complement(8233..8634)
                     /locus_tag="VGT87_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814176.1"
                     /translation="MIDMQDIVKKWSITRAKLEIVSVIVILVCAISVFSVRISNKTSL
                     TYDKGRIHYTGYVINHKMNGEGKLVYPNGDIYEGTFKNGLFEGKGTFTAKTGWLYNGE
                     FHKGQANGKGVLKAKNNKVYKGIFKQGIFQK"
     gene            complement(8657..9094)
                     /locus_tag="VGT87_001329"
     CDS             complement(8657..9094)
                     /locus_tag="VGT87_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEQ3814177.1"
                     /translation="MKKVCFVCLGNICRSPMAEFVMKSIVSSDVMMIESRATSDWEHG
                     NPIHSGTQSILKTYQINYDITKCSKQITITDFNTFDYIIGMDSDNVKNLKEMSQHQWD
                     SKIYLFREGGVPDPWYTNDFEETYQLVRKGCQDWLSRLMSKEY"
     gene            complement(9232..10230)
                     /gene="ruvB"
                     /locus_tag="VGT87_001330"
     CDS             complement(9232..10230)
                     /gene="ruvB"
                     /locus_tag="VGT87_001330"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023079556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEQ3814178.1"
                     /translation="MARILDNNVMGNEEFSDRTLRPQYLHEYIGQDKVKEQFAIFIEA
                     AKRRDESLDHVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPAVEKAGDLVAILNELE
                     PGDILFIDEIHRMPMSVEEVLYSAMEDFYIDIMIGAGDTSRSIHLDLPPFTLIGATTR
                     AGMLSNPLRARFGITGHMEYYQEKDLTEIVERTATIFEIKIDHEAARKLACRSRGTPR
                     IANRLLKRVRDYAQIIGDGIITAQITDRALTMLDVDREGLDYIDQKILRTMIEMYQGG
                     PVGLGTLSVNIAEERNTVEEMYEPYLIQKGFLMRTRTGRVATQKAYRHLGYPYQNT"
     gene            complement(10261..10359)
                     /locus_tag="VGT87_001331"
     CDS             complement(10261..10359)
                     /locus_tag="VGT87_001331"
                     /inference="COORDINATES: protein motif:HMM:NF038244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system DWW-type pheromone"
                     /protein_id="HEQ3814179.1"
                     /translation="MLKKVKPFLLLAAVVAFKVARVMHEFDWWNLG"
     gene            complement(10450..11361)
                     /locus_tag="VGT87_001332"
     CDS             complement(10450..11361)
                     /locus_tag="VGT87_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ3814180.1"
                     /translation="MLEHFGGKVKVLRLEKRISREDLCGDESELSVRQLARIELGQSI
                     PSLSKVIFIAKALNVSVGYLTDGADLELPKRYKELKYLILRTPTYMDDGKLQVREEQF
                     DEIFEDYYDKLPEEEKVAVDIIQAKFEVYQTGDINFGYSILKEFLPQLKRKSIYNLNE
                     LLLIDLYLIILVVSHFSDDIFDVQFYEEITESMLKQHNNLPLEDLFLLNNILLSCADT
                     YIRLKMFGRLKETLQLSHFIMSTIQDFQKMPMYCMYEWKLSIFYLKDINRARNYFEQS
                     ILFTQMTGDTYLVQKLRGEWNKDIHYI"
BASE COUNT         3665 a         2404 c         1848 g         3514 t
ORIGIN      
        1 gtttagctcg tgttcaagtt ccatcaaaca ctaactgtac ttgcaaaatc tcaccgatta
       61 aagtttgatt tctacgttga caccacttgg aagatcaagt ttcatcaaag catcaacagt
      121 tttctgtgtt ggattgatga tgtctaccaa acgtttgtgt gtacgcattt caaattgttc
      181 gcgagaatct ttgtatttgt gagtcgcacg aataattgtg taaagactgc gttcagttgg
      241 aagtggaact ggtccagcaa ctgtagcacc tgtacgtgtt gcagtttcaa cgattttttc
      301 tgccgctgta tcaagtgtac ggtgttcgta cgctttcaaa cggatacgga tttttttgtt
      361 tgccatcttt tctcctctcg tctatttacg ataataggct agctcctcaa gaaaaccgac
      421 acctgttgcg tggcaatgca accgagcgtg tcgcaacctc ttgtatcatt gctaaagctg
      481 tatttttaca gcaccacaat ataataacag aatcatttag tcattgcaag ctttttaggc
      541 atttttttca aaaaaactac cttcctaatg agaggtaatt tcttggtact ttttaaattg
      601 tttagagaga ataaaccacc gactggtatt atctaacact gtcgccatcc agacccctat
      661 gagtccatat tgccaaacca caccaatcac ataaccaagg ccaatgcgaa tcacccacat
      721 tcctatggtt gttgcataga aaggaaactt tgcttttccc aaaccctgcc agacggctgt
      781 ataaactaaa gtgcctgccg ttgcgggagc tcctagtaaa gaaaaaagta aaacaaccat
      841 ggcggacctt tgagcatcag tattctgtgt aaatagcggc aagagactag gacctagcag
      901 ataggtcaag gctcccataa ccagcatcat gagggtggat aaaataaaag cttctctaat
      961 aatatacctt atctcagtta ccttaccgcc ccccagttgc ctagccacta gaataattgt
     1021 tgccgttgcc atggctaagc caggcatgta attaaactga gtcaaggttt ctccaatagc
     1081 attacctgcc aaggccgttg tcccaaagcg aacaacaatg ccaataatta ggacatctcc
     1141 tgctctcatc atcaaacgtt caccagcagc aggtagtgat aaatcaaaaa taattttgtc
     1201 caaaggtctc atcaatcgtt tcacaacctg tttaatagga ataaactgac aaagtaaaaa
     1261 catacccact aaacgagata aaaccgtcgc ccaagcaaca ccaagaagac caaatcccca
     1321 tacatagata gacaaggcag aaaaaatggc attgagtacg tttattaata agctcacttg
     1381 cataggaatc ttaggatacc cttgagctcg aacaatagct cctaagctag ttaaaagtcc
     1441 tagactaaca atcataccgc cgacaatgga tagatattgc cctccaacca atgttacaga
     1501 agcttcagct cctagccatt ctaacacctg atgatgtcca acaactgata acaaccctaa
     1561 gccgaccgaa aggaggagtg ttacttgcaa gacacctgcc atatagttta attgtttgtt
     1621 ctgattattt tctccaatac ttcgagcaat cagactagat acagcagctc ccagcgcgat
     1681 aaagagggac tgataaatac tgataatgtt gttagctatg gagacaccag agactgctac
     1741 aagtccaatt tgagccacca ggtaattatc aaccattccc attaacatct gtaaaatatt
     1801 ttctatcata gaaggcagag ctaaagagaa aatttttctt ctattatata tcatttatgt
     1861 cccctaataa aaaattcttg atcaaacgat tttttcaaac acagtcactg gactcattca
     1921 gttattttgg tccattaatg aattcataaa gtctatctta atagcagagt cccaagatac
     1981 acgacactta gtgttacagt aacaattgac cacatgccaa ttcttaacat ttacctaacg
     2041 tttataacta agaagacgaa gaacaaaagg accccaaacg aattttttct attatataat
     2101 atattcttca tttagcaact ttcaatgtat caaaatagca ctctcgagtc aaaaatggtt
     2161 tctgatactc gagagtgcta ttttatttgg aaccaagttt aagcaactta ggcttatctc
     2221 atatttaata catttgtatg tttagctgat gaaatctaag accttgcgcc cttggattaa
     2281 accacgttcc atttcatcaa agacttctgg agcagtgtca actggaactt tttcgacaac
     2341 cggaacaacc agaccttctg caccaaaggc aaaggcttct tccaagtctt tacgtgtccc
     2401 tacaagtgat ccgaccactt tgataccatc taaaacagtt tttacgatag acagttccat
     2461 gtattctgat ggaagaccta cggctacaac tgtcccaccg gcacggacac tatcaattgc
     2521 ttggttaaaa gcaactttag aaacagccgt taccacaaca ccatgtgctc caccggtttt
     2581 ttcttgaata tagcctggaa catcttcaat ttctttgccg ttaactaaaa tatctgcgcc
     2641 aacttcttta gctaactcaa gtttatcatt attaatgtcc actgcgacaa catgtgcatt
     2701 aaagactttt ttagcgtatt gaacagccag atttccaagg ccacctgccc caaagataac
     2761 aatccattgg cctggagcag cacctgcctc tttaatagct ttataagttg ttacaccagc
     2821 acaagtaatg gaagaagctt gagccgggtc taaaccttct ggaactttaa ctgcataatc
     2881 agctgttact actgcatatt cactcattcc tccatcaaca ctgtatccag catttttaac
     2941 actacgacaa agtgtctcac gtcctgtagt acagtattcg caatgaccac atccttcaaa
     3001 gaaccatgca atagagacac gatcaccaac ctttaaggat gttacgcctt ctccaatttc
     3061 ttctactata ccaatacctt cgtgccccaa aatacgtcct ggtacctgac caaaatcgcc
     3121 atgtgcaacg tgtaaatcag tatgacaaac gccacagtat tctactttaa caagcgcttc
     3181 accatgacca acatttggaa gatcatgctc aacaacttcg acacctgtac tagcttgatt
     3241 aacaactacg gctttcatat cagtcctcca tctaataacg acttatttgt aatcggttac
     3301 aaataaattg tacatctttt cacaattaaa gtcaacagaa acctgaaaag tttctgaaac
     3361 ttaactggtt aatcttatag ctgtttgcct atctgcaaaa tacttaacta cattttctga
     3421 cactggatat aaatgatata tttagaaaat aaaatgattt cttatgactt taatcataag
     3481 aaatcatttc ctggtatcaa cattaaaaat cacttacgtc gtcctggacg ttcagcataa
     3541 ccatagtaag catcttcaat aatttcttgc atgtgatcaa ccattggaag acgtgggtta
     3601 gcaggtgaac attggtcttc ataagcaagg taagccaatt cacgagaatg ttctttccat
     3661 tcattttcgt cgataccttg agctttgaag ttcatttgaa taccaactcg gcaacccaga
     3721 tcataaacag ctttcgcata agattccact gcttcttctg gtgtagaagc tggaagccct
     3781 aacaatttcg caatgtcttg gtatttttca tccgcacggt agtaattata cttaggccat
     3841 gtcgccgttt tagctggacg agttccatta tagcggataa catacggcaa aagaattgca
     3901 tttgtacgtc cgtgtacggt atggaattgt gcgccaattt tatgtgccat tgagtgagaa
     3961 atacctaaga atgcattggc gaaagccata cctgccatag ttgacgcatt gtgcatcttt
     4021 tcacgtgctt caaaatcggc tgttttaact gatttttcaa gattatcaaa aacaatttta
     4081 atggcttgaa gagctaaacc atccgtaaag tcattggcca tttgtgacac gtaagcttcg
     4141 gttgcgtgtg tcaaaacgtc cataccagta tcagcagcaa taaagcctgg aacagtcaat
     4201 accaaagctg ggtcaacaat ggctacagtt ggtgttaatg agtaatcagc gattggatat
     4261 ttacgattat ttgctttgtc agagataacg gcaaatggtg tcacctctga acctgttcca
     4321 gaagttgttg ggatagcaac aaattttgtt ttcttaccaa gttctgggaa tttgaacgca
     4381 cgtttgcgga tatccatgaa tttttgaacg aggtcatgga aatcaacttc tggttgctcg
     4441 tagaagagcc acataacttt ggctgcatcc ataggagatc ccccgccaag ggcaataatg
     4501 gtgtctggtt tgaaggtacg cattaattca gtacctttca taacagttgt aatgtctgga
     4561 tctggttcta cttcagcaaa gatctgataa acaactttat tgcgacgaag atcaagttgt
     4621 tcaatgatac gatccaagaa accaagctca accatagcgt gatctgtaac aatcatgaca
     4681 cgttcaacgt cacgacattt ttgcaaatat tgaattgaat cacgttcgaa gtatgttttt
     4741 gaaggaactt taaaccattg catattattt ctacgtcttc ctactttttt gatgttcaag
     4801 agatttatag cactaacgtt atcaccaact gcgttacgtc catacgaacc acaaccaaga
     4861 gtcaatgatg gcaagaaagc attgtaaaca tccccgatac caccaaatgt agaaggtgaa
     4921 ttccagatga cgcggatagc acggattcta gttccaaatt cttttgccaa ctctgcatct
     4981 gctgtatgaa tggcagctga gtgaccgagt ccattaaatt caaccatttg acgagctttt
     5041 tcaacaccgt cttcacgtga ttcagatttc aaaacagcaa tgactggaga taatttttca
     5101 cgtgttaaag gctcattttc tgaaacttct ttacattctg cagcaagaat attggttcct
     5161 tctggaacag taaatccagc ttgttctgca atccaagtag ctggttttcc tacaatattt
     5221 ggatttaatt ttgcaccagc acagtttttg ctatttgctt tggcaccaaa acagaattct
     5281 tcaagaaggg ctttttcttt tttatttaca aaataagtat ggtaagattt gaactctgct
     5341 acaaaatcgt catagatttc tttatcgatg ataacagctt gttcagaggc acagaccata
     5401 ccattatcaa atgatttaga catcacaata tcatgagcgg cttgacggat attagctgat
     5461 ttttcaacat aagctggaac gttaccagca cctaccccaa gggcaggttt cccacatgag
     5521 tatgccgctt taaccatcgc atttccacct gtcgcaagga tagtggcaat gccgtcatga
     5581 ttcatcaaag catttgttgc ttcaagtgat ggtgtttcaa cccactgcac acaattttca
     5641 ggagcaccag cggcaatagc agcatcacga acaatacgag cggcatgcgc tgatgattct
     5701 tgtgctgatg ggtgaaaggc aaaaataatt ggattacgtg tttttaacga gatcaaagat
     5761 ttaaagatag ctgttgaagt tgggttagtt gttggtgtaa tcccacagat aacacccacc
     5821 ggctcagcaa ttaatgtcaa acctgtcaca tcatcttctt caataattcc tactgttttt
     5881 tgatggcgca tgttattaac cacatgctca caagcaaata gattttttgt tgctttatct
     5941 tcaaagacac cgcgacctgt ttcttcataa gcatgtttag caagttcacc gtgagcatcc
     6001 aaagcagcaa cagaagcttt ggctacgata taatcaactt gttcttggtc aagcttacgc
     6061 atctcttcaa gagcagctaa acctttttga acaagagcat caattgtcac agaaacagag
     6121 gttgtttcaa cagtattatg tccttcagtc atttgagact cctttatctt gttaaaagtt
     6181 tcacaatttt gtttacaaaa ttattatagc actttttgaa attttgtaaa tcatttcaca
     6241 ataaatttgt gaagtttttt tcaaattatt tctgaaatcc tatggtatca atattcttca
     6301 ttaactcttt ttcagaaata tatctaaaaa aagccgtttt atttctgaaa tcgctttctt
     6361 ttaaaaacct caaagtacta ctattttacc atcatcattt gttcacaaga aaaagccaga
     6421 gcacaatgga tctgacttat ctcttagaca tttcttaact ttatttagct ggttttttag
     6481 ctgacttctc aacagcagtc tggattgtgg agacatatag ttctgctcct tgactatctt
     6541 cactataatg aacaccgtca ctgccatacc aaatatctgg atgttcaaca gcaactttat
     6601 accagtctgc aatagtgaca tacttgtatt ttttggacaa tttaagacca tagtctctag
     6661 cttctgcaac ctgactggca tttttggcat tatatggaga aaccaaaact aaacgatgac
     6721 catttggcaa tgcctcaata aaagactgaa ctgcttgaga atagttgtca agcgagttaa
     6781 cacctactgc taagacaaca gtcttagata gactcttaga tttaatttgg ttattaaata
     6841 aatcgaaagc ttctttaaaa ttccgactga ccgctgcatc cagttgcgct tgtggcataa
     6901 tttttgagaa tgctgcacta gagcgtaatg ctacagaatc accaataata cctacgtcac
     6961 tcaaagcatt agcgtctcct gcagctagtg tatgtgtccg ctccatatta ctttgagcct
     7021 gttgtaggga actaaccaac aattgtttct caaacttacc aactttaggc gctatcatac
     7081 aagttcctaa agttaataat gctaaggcga gagtccctcc tacaatccac ttatagtaag
     7141 gagaacagtc aatttctaaa ccaagcaggt ttggttttct cccttggacc aaaggttcta
     7201 caatatagta agacacagtg gcaaacaaga ttgaaaagaa aactgttaga ataactgcca
     7261 aaatatgaga cattaattgg ctaaaaataa tgtaaaatgg ccaatggaag aggtaaatcc
     7321 catagctaat atcagctata taagtgatag ctttaggctc ttgtacatct ggtgtttgct
     7381 catgaagaac acgcgctgca taaatcataa ttgatgcgaa taagctagcc aaagcaaagc
     7441 caaatagata ggtcgtgata tggttaaaat ccaatgtgac cgttagcaca agcaaaagtg
     7501 taaatgctcc aaccatagca gcaaggacat actggcgcgg ccataaacgt acattttttt
     7561 gaaatcgaac agtagtctcg ttaatacctg ttatcgtggc aaacatcgcc cctaaaaaga
     7621 agggaaaact gtgagaaaga ctcgaaaaat aaatcaggga aaaatttgag gtcataaaag
     7681 atcttataaa catgctcaag aaactaatag caaatattcc caaagaaatc aaaaacagta
     7741 gtcctctcag ttgtttctga gtctccttgc gtctagctaa taaccaaaca aatacacccc
     7801 aaaacaagta aaaatgaact tcaatagcta agctccacgt atgaacaaag agatgtggaa
     7861 taaattggct ttcataacta cttcccgtca atatttcata aatattagtg gtaaatccta
     7921 aaacagctgt aatctgactt cctatattag caatgaaatc cttcttaatt aaaaacgtaa
     7981 aaggaatggt caataaaatc attaacacca aaggaggaac aattcgataa aagcgtcttt
     8041 ttaaaaaacc gataatatcg atagactctt tttttgtgta ttcatcaatc aagagggctg
     8101 tgattagata tcctgaaaag gtgaagaaaa tgtcaacacc aataaaacct cctggaaata
     8161 catttttaaa aaagtggtag agtaaaacca gtaaaagtcc tgttacccta acaaacgaaa
     8221 accattttat tctcattttt gaaatatccc ctgtttaaaa atccctttat agactttatt
     8281 attctttgct tttaacacac ctttgccatt tgcttgtcct ttatgaaatt ccccgttata
     8341 taaccagccc gtcttagcag taaaagttcc ttttccttca aataggccat tcttaaaagt
     8401 accctcatag atatcaccgt tggggtagac aagcttccct tctccattca ttttatggtt
     8461 aatcacgtaa ccagtatagt gtatacgccc tttatcatag gtcaaacttg tcttattcga
     8521 aattcttacg ctaaaaacag aaattgcaca aaccaatatg acaatcaccg aaacaatttc
     8581 caacttcgcc cgtgtaatac tccatttttt tactatatct tgcatatcta tcatttcttt
     8641 tactcaactt ctaatatcaa tactctttac tcattaaacg agaaagccaa tcctggcatc
     8701 cttttctaac taattggtag gtttcttcaa aatcgttagt gtaccaagga tcaggaacac
     8761 ccccttctct aaacagatag attttagagt cccattgatg ttgcgacatc tctttgagat
     8821 ttttcacatt atcgctatcc ataccaataa tatagtcaaa ggtgttaaaa tcagttatag
     8881 ttatctgctt ggagcattta gttatatcat aattaatttg gtaagttttt aatatggact
     8941 gtgtacctga gtgaataggg tttccatgct cccaatcaga agtagcacga ctttctatca
     9001 tcataacatc acttgatact attgacttca ttacaaactc agccatgggg cttcgacaaa
     9061 tattacccag acagacaaaa catacttttt tcattaaata ctcctttgaa ttattatatc
     9121 aaatttctaa ctgatatcgg agaaatcaat ctaataattt tctgtcaact tatcaaaaaa
     9181 tccgaacaca aaaagcaggt tcagattaat tttcttagat ttgaaaaatc attaagtgtt
     9241 ttgataggga tagccaagat gacgataagc cttttgagtc gctacacgac ctgttcgtgt
     9301 tcgcatgaga aagccttttt gaattaagta gggttcatac atctcttcaa ctgtattacg
     9361 ctcctcggca atattcacag ataatgtccc caaaccaact ggccctccct gatacatctc
     9421 aatcatcgtt cgtaaaattt tctgatcaat gtaatcgaga ccttcgcggt caacatctaa
     9481 catagtcaaa gctctatcag ttatttgagc cgttatgatg ccatcaccaa taatttgagc
     9541 atagtcacgc actcgtttta gtaatcgatt agcaatgcgg ggcgttcctc tactgcgaca
     9601 agctaatttc cgtgcagctt catggtctat ttttatttca aaaatagtag ctgtacgctc
     9661 cacaatctct gtcaaatcct tttcttgata gtattccatg tgaccagtga taccaaaacg
     9721 agctctgagg ggattagata acatgcctgc acgtgtagta gcaccaatca atgtaaatgg
     9781 aggcagatcc aaatgaatac tacgactagt atctccagcg ccaatcataa tatcaatata
     9841 gaagtcttcc atagcactat atagcacttc ctcgacagac attggcatgc ggtgaatttc
     9901 atcaataaac aaaatatccc ctggttctaa ttcatttaaa atagctacca aatctccagc
     9961 tttctcaacg gcaggacccg aagtttgctt taaattaact cccaattcat tggcaatcac
    10021 aaatgccatt gttgtttttc ccagtcctgg aggtccaaat aataaaacat ggtctaaaga
    10081 ttcgtcacgt cttttagctg cttctataaa aatagcgaat tgttccttaa ctttatcttg
    10141 tccaatatat tcgtgtagat attgagggcg aagagtccga tccgaaaact cctcatttcc
    10201 cattacatta ttgtctaaaa ttctagccat aggactatta taacataaag taaaagagag
    10261 ttaaccaaga ttccaccaat caaattcatg cattacacga gcaactttaa atgcaactac
    10321 tgcggctaat agtaaaaatg gcttaacttt ttttaacatc ttctatctcc caaaaaatat
    10381 tatttataag agatagtata gctatagtaa atataaataa acatgacatc catgtcataa
    10441 aatcattatt taaatataat gaatatcttt attccattct ccccgtaatt tttgaactaa
    10501 atatgtatct cctgtcattt gagtaaataa aatagactgt tcaaagtaat tccgtgctcg
    10561 gttaatatct ttcaaataaa aaatagatag cttccattca tacatacaat acataggcat
    10621 tttttgaaaa tcttgaatag ttgacattat aaagtggcta agttgtaatg tttcttttag
    10681 acgaccgaac atcttcaaac gaatataagt gtccgcacaa gataataaga tattattcag
    10741 taaaaataaa tcttccaatg gtaaattatt gtgttgtttt aacatgctct ctgttatttc
    10801 ttcataaaac tgtacatcaa atatatcatc tgaaaaatga gaaactacca gaataatcaa
    10861 atacaaatct ataagtaata attcatttaa attatagata gattttcttt ttagctgtgg
    10921 tagaaattct tttaaaatac tatatccgaa attaatatct ccggtttgat aaacttcaaa
    10981 ttttgcttgg ataatatcaa cagcaacttt ctcttcctct ggtaatttat cataataatc
    11041 ctcaaaaatt tcatcaaact gctcttctcg tacttgtaat tttccatcat ccatgtaagt
    11101 tggtgtcctt aagataaggt attttaattc tttgtaacgc ttaggtagtt ctaaatcagc
    11161 accatcagtt aagtaaccga cactaacgtt taaggctttt gcaataaaaa taaccttact
    11221 taaacttggt atggattgac ctagttctat ccgtgctaat tgacgaacag aaagttcaga
    11281 ctcatcccca cacaagtcct cgcgactaat cctcttttca agtcttaaca cttttacttt
    11341 tccaccaaaa tgttctaaca tggtttctct ccaacaactg ttttctttat tatatcacag
    11401 cgtctaatca aaaaactaac ctttttaata t
//
LOCUS       DAUCLR010000061        11044 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.061, whole genome shotgun sequence.
ACCESSION   DAUCLR010000061 DAUCLR010000000
VERSION     DAUCLR010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11044
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.061"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(51..269)
                     /locus_tag="VGT87_001333"
     CDS             complement(51..269)
                     /locus_tag="VGT87_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814181.1"
                     /translation="MLLTGKIFLQLLLLRINTKTCDIKGQEKTIKNTLTEVNLDAKSD
                     NRLEDVMVIFIIFSFKMLYFRHVKNLAF"
     gene            complement(220..1218)
                     /locus_tag="VGT87_001334"
     CDS             complement(220..1218)
                     /locus_tag="VGT87_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEQ3814182.1"
                     /translation="MKNKSLIATLLVLTVIVIGSLLSKGVSKENRDLANQQNITIGIL
                     QLVTHEALDDIKRGIEDQLKKQKPQKQNVVIKVMNAEGDQSKIQTMSRQLVQSGSDIV
                     IGIATPAAQGLAAASKDIPVVMSAVSDPVGSRLVMQLDQPEANVTGLSNKVPVKQTID
                     LMKKLTPHVKTVGILYASNEDNSLSQVKEFRRLARKKGYQVISYAVPSTNEVPATMSV
                     MLGKVDAVFIPQDNTIASAFSSVMTTSKAAKIPVYTSVDRMVEKGGLAAISQNQYDLG
                     VQTANQVLKLIKGKRVVDVPVKVVDIGQPLINKNVAAELGIAINREDFPSASFIEN"
     gene            1572..3224
                     /gene="fbp54"
                     /locus_tag="VGT87_001335"
     CDS             1572..3224
                     /gene="fbp54"
                     /locus_tag="VGT87_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein Fbp54"
                     /protein_id="HEQ3814183.1"
                     /translation="MSFDGFFLHHLTNELKENLLYGRIQKVNQPFERELVLTIRNHRK
                     NYKLLLSAHPVFGRLQITQADFQNPQVPNTFTMIMRKYLQGAVIEQLEQIDNDRIIEI
                     KVSNKNEIGDAIQATLIIEIMGKHSNIILVDRAENKIIESIKHVGFSQNSYRTILPGS
                     TYIEPPKTAAVNPFTITDVPLFEILQTQELTVKRLQQHFQGLGRDTAKELAELLTTDK
                     LKRFREFFARPTQANLTTASFAPVLFSDSHATFETLSDMLDHFYQDKAERDRINQQAS
                     DLIHRVQTELDKNRNKLSKQEAELLATENAELFRQKGELLTTYLSLVPNNQDSVILDN
                     YYTGEKIEIALDKALTPNQNAQRYFKKYQKLKEAVKHLSGLIADTKQSITYFESVDYN
                     LSQASIDDIEDIREELYQAGFLKSRQRDKRHKRKKPEQYLASDGKTILMVGRNNLQNE
                     ELTFKMAKKGELWFHAKDIPGSHVIIKDNLDPSDEVKTDAAELAAYYSKARLSNLVQV
                     DMIEAKKLHKPSGAKPGFVTYTGQKTLRVTPDQAKILSMKLF"
     gene            complement(3283..4530)
                     /locus_tag="VGT87_001336"
     CDS             complement(3283..4530)
                     /locus_tag="VGT87_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEQ3814184.1"
                     /translation="MLNSEKMIASLDQQDLAHAEKYFQKALKEDDADSLIALGEYLES
                     IGFLPHAKRIYLQLADDYPELNINLAQIAAEDDAIEEAFLYLDKVSKDSPNYLSALLV
                     MADLYDLEGLTEVAREKLLQAVAISSEPLVIFGLAEIDMSLQYFKEAIDYYAQLDNRQ
                     ILELTGISTYQRIGRAYASLGKFEAAIEFLEKAVAIEYEDETVFELATLMYDQENYQK
                     ANLYFKQLETINPDYPGYEYGYALSLHEEHKTSEALRLVQQGLRKNAFDSQLLLLASQ
                     LSYELHDRQNAENYLLQAKEVAVDDEEILMRLVTLYFDAERFEEVIALNRETIDNVLT
                     KWTIAKAYHALEQEEVALALYNEISADLAENPEFLQDYAYLLREFGQFHKAIQMATAY
                     LRQVPDDVNMQDFLDHIKHQQSE"
     gene            complement(4520..5701)
                     /locus_tag="VGT87_001337"
     CDS             complement(4520..5701)
                     /locus_tag="VGT87_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEQ3814185.1"
                     /translation="MSLIPEKSRTDSLFYKWFLNNQATMALVITLLAFLTIFVFTKIS
                     FLFMPVISFFAVIMLPLVISTILYYLTKPLVDLINHLGPNRTTSIFIVFGLITLLFVW
                     AISGFVPMVQTQLTSFIEDLPKYVGKVNEEANKLLENEWLVSYKPQLQDMLTHTSQKA
                     LDYAQSFSKNAIDWAGNFAGAIARITVAIIISPFILFYFLRDSSHMKNGLVNVLPLKL
                     RVPMVRVLGDINKQLSGYVQGQVTVAIVVGFMFSIMFSLVGLKYAITFGIIAGFLNMI
                     PYLGSFLAMIPVVIMAMVQGPFMLVKVLVIFMIEQTIEGRFVAPLVLGNKLSIHPITI
                     MFLLLTAGSMFGVWGVFLVIPIYASVKVVIKELFDWYKKVSGLYDEEVLVIEEVKDHV
                     K"
     gene            complement(5759..6235)
                     /locus_tag="VGT87_001338"
     CDS             complement(5759..6235)
                     /locus_tag="VGT87_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEQ3814186.1"
                     /translation="MTQLATICYIDNGDSLLLLHRNKKENDVHKGKWISVGGKLEAGE
                     TPDECARREILEETHLTVTEMAFKGIITFPEFTPGHDWYTYVFKVTGFEGDLISDEES
                     REGTLEWVPYDQVLEKPTWEGDYDIFKWILEDRPFFSAKFTYDQNNQLMDKSVTFY"
     gene            complement(6839..7549)
                     /gene="speh"
                     /locus_tag="VGT87_001339"
     CDS             complement(6839..7549)
                     /gene="speh"
                     /locus_tag="VGT87_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptococcal pyrogenic exotoxin SpeH"
                     /protein_id="HEQ3814187.1"
                     /translation="MRYNCRYSHIDKKIYSMIICLSFLLYSNVVQANSYNTTNRHNLE
                     SLYKHDSNLIEADSIKNSPDIVTSHMLKYSVKDKNLSVFFEKDWISQEFKDKEVDIYA
                     LSAQEVCECPGKRYEAFGGITLTNSEKKEIKVPVNVWDKSKQQPPMFITVNKPKVTAQ
                     EVDIKVRKLLIKKYDIYNNREQKYSKGTVTLDLNSGKDIVFDLYYFGNGDFNSMLKIY
                     SNNERIDSTQFHVDVSIS"
     gene            complement(7575..8252)
                     /gene="spei"
                     /locus_tag="VGT87_001340"
     CDS             complement(7575..8252)
                     /gene="spei"
                     /locus_tag="VGT87_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047373492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptococcal pyrogenic exotoxin SpeI"
                     /protein_id="HEQ3814188.1"
                     /translation="MSSVGVINLRNLYSTYDPTEVKGKINEGPPFSGSLFYKNIPYGN
                     SSIELKVELNSVEKANFFSGKRVDIFTLEYSPPCNSNIKKNSYGGITLSDGNRIDKKN
                     IPVNIFIDGVQQKYSYTDISTVSTDKKEVTIQELDVKSRYYLQKHFNIYGFGDVKDFG
                     RSSRFQSGFEEGNIIFHLNSGERISYNLFDTGHGDRESMLKKYSDNKTAYSDQLHIDI
                     YLVKFNK"
     gene            complement(8526..9860)
                     /locus_tag="VGT87_001341"
     CDS             complement(8526..9860)
                     /locus_tag="VGT87_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysin"
                     /protein_id="HEQ3814189.1"
                     /translation="MATYQEYKSRSNGNAYDIDGSLGAQCWDGYADYCKYLGLPYANC
                     TNTGYARDIWEQRHKNGILNYFDEVETMQAGDVAIFMVVAGVTPYSHVAIFDSDAGSG
                     YGWFLGQNQGGANGAYNLVKIPYSTTYPTAFRPKVFKNAVTVIGNIGLNKGDYFIDVS
                     AYQQADLTATCQQAGTTKTIIKVSESLAWLSDRHQQQANTSDPIGYYHFGRFGGDSNL
                     AQREADLFLSNLPTKKVSYLVIDYEDSASADKEANTNAVIAFMDKIANAGYKPVYYSY
                     KPFTLNNIDYQQIIAKYPNSIWIAGYPDYEVRTEPLWEFFPSMDGVRWWQFTSVGVAG
                     GLDKNIVLLADDSSKVDIPKIDKPQSQLTFNQKLDTNTKLDNSNVPYYEATLRTDYYV
                     ESKPNASSADKEFIKAGTRVRVYEKVNGWSRINASQSDQWVEDKYLSNATQV"
     gene            complement(9972..10157)
                     /locus_tag="VGT87_001342"
     CDS             complement(9972..10157)
                     /locus_tag="VGT87_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814190.1"
                     /translation="MNNWFKKVAIKTIKTMAQTAVGLIGSSVLITDINWPTMLSAVLL
                     SGLTCILMNVSQIKEEE"
     gene            complement(10154..10450)
                     /locus_tag="VGT87_001343"
     CDS             complement(10154..10450)
                     /locus_tag="VGT87_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814191.1"
                     /translation="MIDFVQIGAFCGAALSILGVWGFIVNPFKKAMEANEFAMAQLKD
                     SIKELAYELKNLDREREITKKIIDRHEERLGRVEDEVIINKERIITLFKKGEEK"
     gene            complement(10460..>11044)
                     /locus_tag="VGT87_001344"
     CDS             complement(10460..>11044)
                     /locus_tag="VGT87_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814192.1"
                     /translation="YPQYDSTGAVASTHIIITAEDGSVISQPIKQDLTSTNDTEIIKA
                     TLEEFKKSEYVEIAMGEAVQKVDDLEKISQETAKTAKTAQTAAGLAKVSAERTQKMIN
                     LQTIHMLTSGGKIDPDIYKGLLELIEPAKKGEYQAYDVFTVVDESHEEQAGEGNLVFV
                     HVNEPFTYDKQTLEELESEDKVTVIKYADLVKQD"
BASE COUNT         3468 a         2245 c         1816 g         3515 t
ORIGIN      
        1 acgaaactaa gacagaaaac aaaaaagtcg agaatcatgt ttaaacttac tcaaaaggca
       61 agatttttta cgtgtctaaa atagagcatt ttaaaactaa aaatgataaa aataaccatt
      121 acatcctcaa gacgattgtc tgacttggca tccaaattca cctcagtgag ggtgtttttt
      181 atggtttttt cttgaccttt tatatcacat gtctttgtat taattctcaa taaaagaagc
      241 tgaaggaaaa tcttccctgt taatagcaat acctaattct gctgctacat tcttattaat
      301 tagcggttga ccaatatcca caactttgac aggaacatct acaacacgct ttcctttgat
      361 taattttaac acctgattag ctgtttgaac tcccaaatcg tactgatttt gagaaatagc
      421 tgccaaacca cctttttcaa ccattcgatc aacgctagtg tagacaggaa tctttgcagc
      481 cttactggtt gtcatgactg atgaaaatgc acttgctata gtattgtctt gggggataaa
      541 taccgcgtct actttaccta acataactga catcgtggca ggaacttcgt tagtagaagg
      601 aaccgcgtat gaaataactt gatagccctt tttgcgagct aaccgcctaa actctttaac
      661 ttgagataag gaattatcct cattacttgc atataaaatt ccaactgttt tgacatgtgg
      721 tgtcagcttt ttcattaaat caattgtctg tttaacaggc actttattag atagaccagt
      781 tacattagct tcaggttgat ccaattgcat cactaatcta gatcctactg gatcagatac
      841 tgctgacatg acaactggga tatccttgct ggcagctgct aagccttgcg ccgccggggt
      901 tgcgatacca atcacgatat cacttcctga ttgcactaat tgcctactca ttgtttggat
      961 tttactctga tccccctcag cattcataac cttaatcact acattttgtt tttgaggctt
     1021 ctgctttttg agttgatctt caatacctcg cttaatatca tcaagagcct catgagtaac
     1081 caattgtaag atgccaattg taatgttttg ttggttagcc aaatcacggt tttcctttga
     1141 tactcctttt gataatagag agcctatcac tattacagtt aaaacaagga gggtagctat
     1201 taaactcttg tttttcatct gaattcctca catttcatgt caatctcttt aaaacgttga
     1261 tgatgagaat acttctcaaa agaaaaagca cttagtaatg attactaggc gctcttgtga
     1321 aatccaatgt cagcgcatag atatcttttt agaagtactc taaaagtcgt atctatgcca
     1381 attcataaac acaacttatc tatgccagct ttgccaattt gctaagtgtt gtgtttttac
     1441 catcattgac ttcctttctc gtttttttaa cattataacc taaatcaaaa aaattgcaag
     1501 ctctttcgtc catattttta gtcaagtatt tgtggtataa tgaagagatt agaattcaga
     1561 aaggagaaac catgtctttt gacggctttt tcttacacca tttaaccaac gaattaaaag
     1621 agaacttgct ttacggtcgt atccaaaagg tcaatcaacc ctttgaacgg gaacttgtct
     1681 taaccattcg taaccatcgc aagaattata aattgctatt gtctgcccat cctgtttttg
     1741 gacgacttca aattacccaa gcagactttc aaaatcctca ggtacctaat acctttacca
     1801 tgattatgcg taaatacctc cagggtgcag tcattgagca gttagaacag attgataatg
     1861 accgcattat cgaaatcaag gtatctaata aaaatgaaat cggagatgct attcaggcaa
     1921 ccctaatcat tgaaatcatg gggaagcaca gcaatatcat cttggtggac cgtgcagaaa
     1981 ataaaatcat cgaatccatt aaacatgttg gcttttcaca aaactcttac cgcaccattt
     2041 tacctggctc tacctacatt gagcccccta aaacagctgc tgttaatcct tttacaatta
     2101 cagatgttcc tctatttgaa atcctccaaa cacaagaact aacagttaaa agattgcaac
     2161 agcattttca aggcttaggg cgagacaccg caaaagagtt ggccgagctt ctgaccacag
     2221 acaaattaaa acggttccgt gagttttttg ctagacctac tcaggcaaat ctgaccacag
     2281 cttcttttgc gcctgttctt ttctcagata gccatgcgac gtttgagact ttgtctgaca
     2341 tgcttgacca cttttatcaa gacaaggctg agcgcgaccg tatcaatcag caagccagtg
     2401 atttgattca ccgtgtgcaa actgaactgg acaaaaatcg caacaaacta agtaaacaag
     2461 aagctgaact tttggctacg gaaaatgctg aattattccg tcaaaaagga gaattattga
     2521 ccacctacct cagtctggtc ccaaacaacc aagactctgt gattttagac aattattata
     2581 caggggaaaa aattgagatt gccttagaca aggctctgac accaaatcaa aatgctcaac
     2641 gttattttaa aaaataccaa aagctaaaag aagctgtcaa acacttatct ggactgattg
     2701 ccgataccaa gcaaagtatt acttactttg agagcgtgga ctacaattta tctcaagcaa
     2761 gtatcgacga catcgaagat attcgcgaag aattatatca ggctggcttt ttgaaaagtc
     2821 gccaaagaga caaacgccat aaacgtaaaa agcctgagca gtacctggct tcagatggaa
     2881 aaaccattct catggtggga cgcaataact tgcaaaatga agaactcacc tttaaaatgg
     2941 ctaaaaaagg agaactctgg ttccatgcta aggatatccc tggaagtcat gtcatcatca
     3001 aagacaatct tgacccaagt gacgaggtta aaactgatgc tgccgaacta gctgcttatt
     3061 attcgaaagc taggttatca aatctcgttc aggtcgatat gattgaagct aaaaaattac
     3121 acaaacctag tggcgccaag ccaggatttg tgacttatac tggtcaaaaa accttacggg
     3181 ttactcctga ccaagccaaa attctctcta tgaaattatt ctgataagca actaaaaaac
     3241 agctctccta ggaaagttat ttcccctagg agaactgttt ttttattctg attgttgatg
     3301 cttgatatgg tcaagaaagt cttgcatatt cacatcatca ggtacttgtc tgaggtaagc
     3361 ggtagccatt tggatagcct tgtgaaactg gccaaactcc cttaataaat aggcataatc
     3421 ctgtaaaaac tctggatttt ctgccaaatc agccgaaatc tcattataaa gagccaaggc
     3481 aacttcttct tgttccaagg cgtggtaagc ctttgcaatg gtccatttgg ttaagacatt
     3541 atcaatcgtc tcacgattaa gagcaataac ctcctcaaaa cgctcggcat caaaataaag
     3601 ggtcactaaa cgcatgagaa tctcttcgtc atcaacagct acttcctttg cttgaagcag
     3661 gtaattttca gcattctgcc tatcgtgtaa ttcataagaa agctgcgatg ctagtaataa
     3721 taattgactg tcaaatgcat ttttacgtag gccctgttga accaagcgca aagcttctga
     3781 cgtcttatgc tcttcatgaa gactcaaagc ataaccatat tcataacctg gataatctgg
     3841 attgatggtt tccaactgtt taaagtaaag attggctttt tgataatttt cttggtcata
     3901 catgagggtc gccaattcaa aaacagtttc atcttcgtat tcaatggcca cggccttttc
     3961 caagaattcg atggctgcct caaatttccc taagctagca taggctcgtc caatgcgttg
     4021 ataagtggaa atacctgtca attcaaggat ttgacgattg tctaactgtg catagtaatc
     4081 aatagcttct ttaaaatact gcaaggacat gtcgatttct gccaaaccaa aaatgaccag
     4141 tggctctgag ctgatggcca ctgcttgaag caatttttca cgcgccacct ctgtcagtcc
     4201 ttccaggtca tagaggtcag ccatgactaa gagagctgac aaataattag ggctatcttt
     4261 tgaaacctta tccaaataca agaaagcttc ttcaatggcg tcgtcttctg ctgcaatctg
     4321 agctaaattt atattgagtt ctgggtaatc atcagctaac tgcaaataaa tacgcttggc
     4381 atgtggcaaa aaacctatac tttctaagta ttctcctaag gcgattaaac tatctgcatc
     4441 atcttctttt aaagcttttt ggaaatattt ttcagcgtgc gctaggtctt gctgatccaa
     4501 ggaagcaatc attttttcac tatttaacat ggtccttaac ctcctcaatc accaatactt
     4561 cttcgtcata caaaccgcta acttttttgt accagtcaaa taattcttta atcacaactt
     4621 tgacagaagc gtagattgga atgacaagga aaacacccca gacaccaaac atagagcctg
     4681 cagtcaataa caagaacata atggtaatag gatgaatgct cagcttattt cccaaaacaa
     4741 gcggtgctac aaaacgccct tcgatggttt gctcaatcat aaagataaca agcaccttaa
     4801 ccaacataaa tgggccttgt accatagcca taatcacaac aggtatcatg gcaaggaaac
     4861 ttcctaagta aggtatcatg ttaagaaagc cagcaataat cccaaaagtg atggcatatt
     4921 tcagtcccac aaggctaaac atgatagaaa acataaagcc gacaacaatt gcaaccgtaa
     4981 cctgcccttg gacatagcct gataattgct tgttaatatc tcccaagact cgaaccatag
     5041 gcacccgtaa tttgagaggt aagacattca caaggccatt tttcatgtgg ctactgtctc
     5101 gtaaaaaata aaagagaata aagggcgaaa taataatagc cacggtaatc cgagcaatgg
     5161 caccagcaaa attaccagcc cagtcaatag cattttttga aaaactttgc gcgtaatcta
     5221 aagccttttg acttgtatgg gtaagcatgt cttggagttg gggcttgtag ctgactaacc
     5281 attcattttc aagaagttta ttggcttctt cattgacctt gcccacgtat ttgggcagat
     5341 cctcaataaa actggtaagt tgggtctgta ccatgggaac aaagcctgaa atcgcccaga
     5401 caaaaagtaa ggtaatcaaa ccaaaaacaa taaaaataga ggtggtacga ttcgggccca
     5461 agtgattaat aaggtcaact aagggtttgg tcagataata cagaatggtc gaaataacca
     5521 agggcaacat gatgaccgca aaaaaagaaa tcactggcat aaataaaaaa gagatcttag
     5581 taaaaacaaa aatcgttaaa aaggctaaaa gtgttattac taaagccatg gtagcttgat
     5641 tatttaaaaa ccatttgtaa aataagctat ctgttctaga cttttcaggt attaaggaca
     5701 ttaagatagg gttcctttct ttgagaaaat gataagtttt actggtaacc tgggtaaatc
     5761 aataaaaggt cacagattta tccatgagtt ggttgttttg gtcataggta aatttggcag
     5821 agaaaaaagg acggtcttct aaaatccatt taaaaatgtc ataatcacct tcccaagttg
     5881 gcttttccaa cacttgatcg taaggtaccc attccaaggt tccttctcgt gactcttcat
     5941 cggaaatgag gtctccttca aatcctgtta ctttaaaaac ataggtatac cagtcatgac
     6001 caggggtaaa ttctggaaaa gtaatgattc ctttaaaggc catctctgta acagtcaaat
     6061 gggtttcctc taaaatctca cgacgggcac actcatctgg ggtctcacca gcctcgagct
     6121 tacccccaac tgaaatccac ttgcctttgt ggacatcatt ttctttttta ttgcggtgta
     6181 ataatagcaa agaatcgcca ttatcaatgt aacaaatggt tgctaattga gtcatctatc
     6241 tctttccttt ccatctttgt ttctattata ccaatctcta gaaaatatgc tatcatttta
     6301 tgagtcaaag tgtgaatact tagtcacgct caaaaccttc taaagatgcc ttaaaaaagt
     6361 gtagataaga gcctacaatg aaaaacatca atttaaacta tcaaaagaac cttgtcatat
     6421 caacgataat tgatatgaca aggctcttta atgttttatt taatcacttc ttgagtttat
     6481 tcccgaaaaa acacaaggaa caataatggt tacaaagtcg ataataaaag catttgttag
     6541 tattaaaccg gtattgtatc gacacaagaa acgacaaaag ttctaaactt atgccccaat
     6601 tttgccccaa tggttctttt ttgattcttt ggtgaactaa aattattcag actcagaaag
     6661 ttctgccata acatctgata tccgctcgat tgtcacaacc tcccaatctc ttatctcctc
     6721 atatatttta tattaaaata tcaataaata ttagcataat tttaaaacac taaaaatata
     6781 tcttggtaat ttttacttta tttatctatt tttaataaac agcttcttaa aaatataatt
     6841 agctgattga cacatctaca tgaaattgag ttgagtctat tctctcgtta ttggaatata
     6901 tttttagcat gctattaaag tctccattgc caaaataata caaatcaaaa acaatatctt
     6961 tacctgaatt taaatctaag gtaacagttc ctttagagta tttttgttcc cggttattat
     7021 agatatcgta tttcttaatc aataactttc taacttttat atccacttcc tgagcggtta
     7081 ctttcggttt attgactgta ataaacatag gcggctgttg tttactttta tcccacacgt
     7141 ttacaggaac tttaatttct tttttttctg aattagttaa tgtaattcca ccaaacgctt
     7201 catacctttt ccctggacat tcacaaacct cttgtgcaga tagagcataa atatctactt
     7261 ctttatcttt gaattcctgt gatatccaat ctttctcaaa aaaaactgac aaatttttat
     7321 ccttgacact atatttcaac atatggcttg ttacaatatc tggagaattt tttatactat
     7381 cggcttcaat caagttagaa tcatgcttat aaagcgattc tagattatgt ctattggttg
     7441 tattataaga atttgcttga acaacattgg aatataaaag aaatgacaaa catataatca
     7501 tgctgtagat tttcttatca atatgtgagt agcgacaatt atatctcatt ttttctcctt
     7561 aagaagtgag agttttattt attaaattta actaagtata tatcaatatg aagttgatca
     7621 gaataagcgg tcttattatc actgtatttt tttagcatac tctctctgtc accatgtcct
     7681 gtatcaaaaa gattataaga aattctctct ccactattca aatgaaaaat aatatttcct
     7741 tcctcaaagc cagattgaaa tctagaggag cggccaaaat ctttaacatc accgaatccg
     7801 tatatattaa aatgtttttg aagataatat cttgatttca catcaagttc ctgaatcgta
     7861 acctctttct tatcagtact cactgtactt atatctgtat agctatattt ttgttgaacg
     7921 ccgtctatga aaatattaac aggtatattt tttttatcaa ttctattacc gtcgcttaaa
     7981 gtaatacctc catatgaatt cttttttata ttagagttac agggaggaga atactccaaa
     8041 gtaaatatat ccaccctttt accagaaaaa aaattagctt tttctacgct attaagttct
     8101 acttttaatt caatcgaact attgccataa ggaatatttt tgtagaacaa actacctgaa
     8161 aatggcggac cttcatttat ttttcctttt acttctgttg gatcatatgt agagtataaa
     8221 ttccttaaat taataactcc cacactactc atctcatacg cgtaacacgt tggaattata
     8281 gctgatataa atacccacaa tagaaaagat gaagttaaaa actttttaaa attaacattt
     8341 acaatttttc tcacttataa gtgtacccta ttcttcttta ataataagaa atcacaaaat
     8401 attttagcac aaaaaagaaa aaaatcaacg aaaatgctta aataacaatt aaaatcttta
     8461 taacaaaaac agcccccgca aaagcgaggg catttgtctt atctaaagga gctttacctc
     8521 ctattttata cttgtgttgc attagataaa tacttatctt cgacccactg gtcagactga
     8581 gaagcgttaa tacgtgacca tccattcact ttttcataga ctcttacacg agttcctgcc
     8641 ttgataaatt ctttatcagc gctacttgcg tttggcttag actctacata atagtctgtt
     8701 ctaagggttg cttcgtagta aggtacattc gagttgtcta atttagtgtt agtatctagc
     8761 ttttgattaa aagtaagctg gctttgtggt ttgtcaatct taggtatatc cactttgcta
     8821 ctatcatctg ctaataatac aatattttta tctaaaccac ctgctactcc tacacttgtg
     8881 aactgccacc agcgcacacc atccattgaa gggaagaact cccaaagtgg ctctgttcgt
     8941 acttcgtagt ctggataacc agctatccaa atgctgtttg ggtacttagc gataatttgc
     9001 tgataatcaa tattattaag cgtaaatggt ttatagctgt aataaacagg cttatatcca
     9061 gcgtttgcaa ttttatccat aaacgcaata actgcattag tgttagcttc tttgtcggca
     9121 cttgcagagt cttcataatc aatgactaag taagagactt ttttggttgg taaattggac
     9181 agaaataaat ctgcttctcg ttgcgctaag ttgctatctc ctccaaatcg tccaaagtga
     9241 taataaccaa tagggtcact agtattagct tgttgctgat gcctatcaga cagccaagcg
     9301 agtgactcgg atactttgat aatcgtttta gtagtgccag cttgctgaca agtagcggtt
     9361 aagtctgctt gttgataagc tgatacatca ataaaataat cgcctttatt tagtcctata
     9421 ttacctataa cagtaactgc atttttaaaa acttttggtc taaatgcagt aggataagtc
     9481 gttgaatatg gtatttttac taaattatat gctccgtttg ctccaccctg gttttgacct
     9541 aaaaaccatc cgtatccact acctgcatca ctatcaaaaa tagccacatg gctatacggt
     9601 gtaacacctg caactaccat aaaaattgcg acatctccag cttgcatagt ctctacttcg
     9661 tcaaagtagt ttaaaatacc atttttgtga cgttgttccc atatatccct tgcatatcct
     9721 gtatttgtac agtttgcgta tggcagtcct agatacttac agtagtctgc gtagccatcc
     9781 caacattgcg caccaagcga tccatcaata tcgtaagcat tgccatttga acgactttta
     9841 tattcctgat atgtagccat ttatccctcc ttatctaaaa atggataaaa aatcaaagca
     9901 accacagata atggcacata cagtattgcg attgctataa ctaatgctaa tcgtgtgatt
     9961 gctcgcatga cctattcctc ttccttgatt tgtgacacat tcatcaggat acatgttagt
    10021 cctgacaaca gcactgctga caacattgtt ggccaattaa tatccgtgat taacacgctt
    10081 gacccgatta gaccaacggc cgtttgtgcc attgttttaa ttgttttaat tgctactttt
    10141 ttaaaccagt tattcatttt tcttctcctt ttttaaatag cgtaataata cgctccttgt
    10201 tgataataac ttcgtcttct acgcgaccta aacgctcctc gtgtctgtcg ataatttttt
    10261 tggtaatctc acgttcacga tcaagatttt taagctcata agctaactcc ttaattgagt
    10321 ccttgagttg agccatggca aactcattag cttccatggc ttttttaaag ggattgacga
    10381 taaatcccca aacacccaag atagacaaag cagcgccaca aaaagcacca atttgtacaa
    10441 aatctatcat ctaaccacct caatcctgct taactaagtc tgcatatttg ataactgtta
    10501 ctttgtcttc tgactctagc tcctctaagg tttgtttgtc ataagtaaat ggctcgttaa
    10561 cgtgtacaaa gacaagatta ccttctcctg cctgctcttc gtgcgactcg tctactaccg
    10621 taaagacatc ataggcttga tactcacctt ttttggctgg ctcgattagc tctaaaagac
    10681 ctttgtaaat atcaggatca atcttgccgc cgctcgttaa catgtggatc gtttgtaagt
    10741 taatcatctt ttgcgtgcgc tctgcggaca ccttagctag tccagcagct gtttgagcag
    10801 ttttggcagt cttagcggtt tcctgtgaga ttttttcaag gtcgtctacc ttttgcacgg
    10861 cttcgcccat tgcaatttca acgtattcag attttttaaa ttcttccaaa gtagctttga
    10921 taatctctgt gtcattagtt gaggttaagt cctgcttgat tggttgagag atgactgagc
    10981 catcttctgc tgtgataatg atgtgtgtac ttgcgactgc tcctgtgctg tcatattgtg
    11041 gata
//
LOCUS       DAUCLR010000062        10884 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.062, whole genome shotgun sequence.
ACCESSION   DAUCLR010000062 DAUCLR010000000
VERSION     DAUCLR010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10884
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.062"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            78..698
                     /locus_tag="VGT87_001345"
                     /pseudo
     CDS             78..698
                     /locus_tag="VGT87_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein"
     gene            878..1188
                     /locus_tag="VGT87_001346"
                     /pseudo
     CDS             878..1188
                     /locus_tag="VGT87_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670034.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
     gene            1186..1470
                     /locus_tag="VGT87_001347"
                     /pseudo
     CDS             1186..1470
                     /locus_tag="VGT87_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001883480.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1516..1728
                     /locus_tag="VGT87_001348"
     CDS             1516..1728
                     /locus_tag="VGT87_001348"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814193.1"
                     /translation="MMTDRVMKKPIRGRQLSKRDLYDEEFFRTHFAKIEIESRLKFLF
                     NRANSLEELLLKAKEVNLTIDLKQKM"
     gene            1993..2690
                     /locus_tag="VGT87_001349"
                     /pseudo
     CDS             1993..2690
                     /locus_tag="VGT87_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic (srt) production protein"
     gene            2841..3527
                     /locus_tag="VGT87_001350"
     CDS             2841..3527
                     /locus_tag="VGT87_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018373772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ3814194.1"
                     /translation="MYKILAIDDDKEILKLMKTALEIENYHVITCQEIELPIVFDDFK
                     GYDLILLDIMMPNISGTEFCYKIREEVHSPIIFVSALDGDNEIVQALNIGGDDFIVKP
                     FSLKQFVAKVNSHLKREERAKIKNEAEERVKRSFPPIEIYLEERMLYIDKQPLFLTYR
                     EYEILELLSRHPYKVFTKEEIYEQVYSDEASALFHSISEYIYQIRMKFSSFGINPIKT
                     IRGIGYKWDV"
     gene            3520..4866
                     /locus_tag="VGT87_001351"
     CDS             3520..4866
                     /locus_tag="VGT87_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEQ3814195.1"
                     /translation="MFKKYSIKNRVWRAVVEIVFGVCITVLMIAIISLSFSKLNIVTS
                     HNVGEEFYIKDKQSIKQLNNYMKTLGLDYVVFDRKTDKAMEGKYLSKEFSLFNEVAEE
                     KNNLTFNSVHYDLYTNINYNIVIRYNEIPEFSNHYLRNVSYNMLTFYILGIGTSISIV
                     VALTRFVKEISLNFKEIKKLANKMGIEVLSENENYSKIIEFDDILRTLHIKGDNLKSL
                     IEREILEKQDLSFQIAALSHDIKTPLTVLKGNLELLELTTLNKNQEGYIVSMNNSISV
                     FEGYFNSLISYTRMLSEDRSVKLILVEELLSELHFEVDDLLNINNIEFSICNRLIITS
                     FYGDEENLIRALSNLLVNAIRFMPVLDKKIEVILSESGEQIHFEIWNNGERFSDSTLK
                     KGDKLFYTEDYSRGNKHYGIGLAFVKGVAIKHGGNLQLNNPARGGASAIISIKKKI"
     gene            5023..5163
                     /locus_tag="VGT87_001352"
     CDS             5023..5163
                     /locus_tag="VGT87_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic streptin"
                     /protein_id="HEQ3814196.1"
                     /translation="MTNTIKDFDLDLKTNKKDTATPYVGSRYLCTPGSCWKLVCFTTT
                     VK"
     gene            5278..7068
                     /locus_tag="VGT87_001353"
     CDS             5278..7068
                     /locus_tag="VGT87_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ3814197.1"
                     /translation="MIKAQLKNLIKTIHLVKEAGFSLLVFSIAISLFQGILPIFSMLL
                     VQKMLNIITTDIKNFHTLMIAFISYIALTLLTIIIGEVDSYIDTKLQILLHYKMNHLV
                     MQKTVKLTLAEFETPEIYDDITRIQNQISYKPFQIYKSIISVLSSLVSLISSFVILLN
                     WKISIFPLLLILPIVSIYIYLKIGKNEFEILYKRSSDERANWYISHLLTHDFAVKELR
                     LGTLESYFLKKYDKNNQLFINQENSINRSKMKYGIVLGVLEIAIQSIIMFLAVREAFL
                     GILLVGNVTTFIRSLSTMQTSTQNIVNNIYSFYNGSLYMELLYDFTHDELVHNPIIES
                     TVDTIQSVEFERVSFSYNGKDNVINDISFSIKAGERLAIVGENGSRKSTIFKLACGLY
                     DNYEGNIYINGINLRSIQKKSYYKRISALFQDFLKYELTLRENVGLGELSKLYSDEDL
                     ISALKYTGVDSIFYFNQEKLNQIDLEQQLGNWFEDGRQLSGGQWQKIALSRVYLKEAD
                     CYLLDEPSSALDPESETKIFKTFFELSQNKIGIFITHKPSITQSVNKILYLDNGCISE
                     TGSFADLNIKGMKFKNLLDKEIGATYDTSN"
     gene            7052..8296
                     /locus_tag="VGT87_001354"
     CDS             7052..8296
                     /locus_tag="VGT87_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase C family protein"
                     /protein_id="HEQ3814198.1"
                     /translation="MTPQIKQIVDYIADSFINYEQIVAESENKVLINIESKYRNTFFD
                     KSLSHGVPSLILMYSSLYKVTKDERYLTFSNIYVEKLVDIISRDGIESPSLYAGTAGI
                     ALAIKEASISGKYYPKLLSSLNCLLKEQIKDKLVVSFSNIEKGIIEPYDYDMVNGFSG
                     ITNYLLLEQEFFSVELNQIGNYLLKYIETILNKVTNCSEDREAEFDLGIAHGITGPML
                     ILAKLKSEKILSVEVGDILNKAINLMFLFRRDDKLWPGKIYTSNILDLDLNYLPTRMA
                     WCYGMPGTAVALFKMCQLIDLNFIDDINESLIAQMRSSDKNVYSLTMCHGLAGVTFIY
                     DLIGKNYSSERLTRFAEELRQQIITSFSTTKVFGYTDREKFGEIEVELESIGILQGVS
                     GIVLFLLNPYSDEELLWQRIFI"
     gene            8315..>10884
                     /locus_tag="VGT87_001355"
     CDS             8315..>10884
                     /locus_tag="VGT87_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HEQ3814199.1"
                     /translation="MYKVFPTGMIRIPLQKQEFYNKDYEDLINDKVFMEQLLVASPSL
                     YDSVIDFDKCNEKKKQNVKVSLNSYSKRAAFRTTPFGIFTAINTVDLTKGTTSNVQKV
                     SFIKKAVPDYSWIYSLVKSYEISNLEKLSLKINTAAFTQGDRYVLPFTVNESEEDRNI
                     SFSKPIKLLVEKCKTRYIKYEELIDVFKNNYPDIVSDMLESYIHDLVTNDFLISDLRP
                     PICNINSLDYLLSKLEEGTLCTDLTTLKKMIEDYNDLNIGEGRDLLLQIFDKMESIYK
                     YDNGGNYLKVDSLAEYEGDFNILSETARESIERLAEMFVKLNTNVKRRGGRKNSLEEY
                     QLKFIGKYGENCSIPFVEVINKDAGIGFPEYYKGGEGEAIELSDPIMQMFEKKYEEAL
                     LNGGHIEFYSKDLDDFQTDQSNSLDSFELNFNIKIINNDVKLYLGANIGSGQAGRSFG
                     RFYGLSETVRETIKNLNHQNNTNVELSFVPKQIRLANVIQNYSDESYNTSVFTTSWDS
                     ENELRLEDIYIRYSDGKFHFTTIDGKNELKFTMNNMLNSDSQSRVLRLLVDLSEFEYG
                     LSHWSLFPWDILAQERVYIPEILFEDIPIATAQWNLSVIREEIIHQKIFTGKKDIFNK
                     FRKTYKIPKDIILKYADNELRLDLSIDSDLEILFKNINKYRFVSLRGIEQGESIFKDN
                     LGNYNTEIVVPILKESNGINNTNDNVQFKNEVKKIYLPFCDWLFFKFYGPKERQEELL
                     NNWKKFWLNLPSELFSQSNMFYMRYNDTNDHIRIRINCDSIENNFSLYLSVVQDLLPQ
                     LIESCIISDIEVSSYKPEVNRYGGPHLISYAEEIFCKESILFLNNTIEFSDDERLVCA
                     T"
BASE COUNT         3972 a         1345 c         2040 g         3527 t
ORIGIN      
        1 tgtctagtaa taattttttt atgttcagga ggaaaatcat gcaaatgaat gaacagtcat
       61 cacgacaaat ggtgattgcc atctatctca tttattccaa ctcaaaatga tagtactcct
      121 tggctgttcc aagtcgaaat tgttagtgat taagaaaacc tacgagacta tggtttgatc
      181 gatgaaggcc agcagtctcc cagtgaaaaa gggcgtttgg ctaataagat ttatttagga
      241 aagttggaac atgattccac cccagtctta agttcagacg ggggtagtct aaaaaaacac
      301 gggaccagtc tcaaaaacag acaagccccg tgttaaattc agcccctagt gagactgaaa
      361 agagtgaaac tgaaggtagt gaaactaaag agagtaattt agtcattgag gacgaggagg
      421 aaagaaagga gtgcactagt gtgaaaaaga ctgagggtca ctttacccgt caagttgatc
      481 aggtcaccaa atatgacaaa gattatattt ggagtttggt tcattcccaa ttacgtgaag
      541 gtggtttatc acaagctgct agtgacttag tcatgagcta ttttgaagaa cgctatgctt
      601 atgcgctgga acacatacgg ttcgcaagaa cagctgaagc tattgcggaa tatgtcttta
      661 atggtgtctt atccgaatgg accaagcaac taaggtgaca agaagcgaag tgaggtgagt
      721 aagatgtttt ggtggctatt attaggtgtt tggggtctaa ggacaatttg gttgattatt
      781 gaagttatca ctgcaccaga aatggaagat tatagttgag taaggacgtt acataagcag
      841 taaggtcctt ttattttgtc agaaaggaat gagagatatg aagtttttag atttgtttgc
      901 aggaattgga ggctttcgat tgggtttaat taatcagtgc catgagtgta ttggcttttg
      961 tgagattgat aagtttgcga ggcaatctta caaagccatc tatgagaccg aaggagaaat
     1021 agaatttcat gacattagac aagtcacaga ccaagacttt agacaactta gagggcaagt
     1081 ggatatcatc tgcggtggtt tttcccttgt caagcatttt cactcgcagg cagacgactg
     1141 ggatttgagg gcactaggag aactctcttc tttgagatta ctcgagcgac tcatacagac
     1201 aaagatcata ttcataatca catcttaatc aacgccattg actgtaattc agataaaaag
     1261 ttgatatgga attatgcctt ggaacgaaac cttcgtatga tttcagaccg catttctaaa
     1321 atggctgggg cgaaaattat tgaaaagcat ttctcttacc gtgactatca aaaatataga
     1381 gcgactagcc ataagtttga actaaagcaa cgtctttatt ttttgatgca gcagtcaaag
     1441 ttctttgatg attttttaga aaaagcagag taattacatg ttcatattga ttttagtcag
     1501 aagcatagca gattcatgat gacggatcga gtaatgaaaa aaccaattcg aggtcgtcaa
     1561 ctcagtaaac gagatttata tgatgaagaa ttttttcgta cacattttgc caagatagaa
     1621 attgaaagtc gattaaaatt tttgtttaac cgtgccaatt cattagagga gctactatta
     1681 aaagcaaaag aagtgaatct aaccattgac ttaaaacaaa aaatgtaact tttatcctag
     1741 aagaagatac tcaaaagata agtttaggtc ataaaaaaat aagcgataag aaattatatg
     1801 atgtcaattt tttcaagatt actttaaaaa taaggaagtc ggtgtttcag atcgattaga
     1861 aagtctacag aaacagtaca tgcttttcaa gaagaatgag ataaagagga ggtatccact
     1921 gaagagagta aggaagtctt caagaaatac aaggggcaac gagatgccat ccatgagttt
     1981 gaagttgaac ttgtggaaca tcaaattgag aggttaattg ctgaggacat ttatatcaag
     2041 gtgtcttttg gtgtaaagca gagtggtctt attttcattc ctaactatca attggatatt
     2101 aggaaagaag aaaatcataa aaaatataaa gtctatattc gtgagaccgc acaattcttc
     2161 atttacaaca aggaggcgtc taaacttaat cgctatgtgc gagggcgtga gctcatttac
     2221 cagttaacca atgatagcaa gagtattcca aaaagaagac gaccaaaacc attgatacac
     2281 ttaagaaaaa gattgaagaa ataagtctcc tcattgagtt agatacggag aataagcctt
     2341 atcaagatat taaagatgac attgttaaag atatggctca attagatttg accattacag
     2401 agttgcagga ccatattgct catctcaata aggttgcgga ggtcttgctt aatctgaaca
     2461 acaacgatat agagaaccgc cgtttggcca gatatgacta tgccaagatg aacttgactg
     2521 cagctataaa aatagaagaa gttgagaaag agattgaaac ttctcaaaat gaacttaata
     2581 tatccataga tgagtatgaa tatctagtaa gaaggttgga aaagtttgga gagatcttga
     2641 gtgatagcaa aattatcgat acttctcgaa atgaaataca atgggagtaa ttatgatata
     2701 atttgggtgg tagcaatttt tatatcagat attaaaatct agttgatgtt tcgtaaataa
     2761 ctatattaaa gaaatgattc ctgccgctta cattaagaat agtttatttg taagtatttc
     2821 attatttaaa aggggtaata atgtataaga ttttagccat agatgatgat aaagaaattc
     2881 tgaagcttat gaagactgct ttggagattg aaaattatca tgttataacc tgtcaagaga
     2941 tagaattacc aatagttttt gatgatttta aaggatatga tttgatctta ttggatatca
     3001 tgatgcctaa tataagtgga actgagtttt gttataaaat tcgggaagaa gttcattctc
     3061 caattatttt tgttagcgct ttagatggcg ataatgaaat tgtccaagct ttaaatatag
     3121 ggggagacga ttttattgtg aaaccattta gcttaaaaca attcgtagct aaagttaact
     3181 ctcatttgaa gagagaagag agagcaaaga taaagaatga ggctgaggag agagtgaagc
     3241 gcagttttcc acctatagaa atctatctag aggaacgtat gttatatatt gataaacagc
     3301 cgttattcct gacttataga gagtacgaaa ttttagaatt actgtcacgt catccgtata
     3361 aagttttcac aaaagaagag atatatgaac aagtatatag cgatgaagct tcagcattgt
     3421 ttcattctat ttcagaatat atctatcaga ttagaatgaa attttcaagt tttggaatta
     3481 atccaataaa aactattcgc gggattggtt ataaatggga tgtttaaaaa atattcaata
     3541 aaaaatcgtg tttggcgcgc cgtcgttgaa attgtctttg gagtttgtat cactgtttta
     3601 atgattgcga taatatcgct ttctttttca aagttaaata tagtgacatc tcataatgtg
     3661 ggagaagaat tttatattaa agataaacag tcaatcaaac agttgaacaa ttatatgaag
     3721 acattgggat tagattatgt tgtttttgat agaaaaacag ataaagctat ggaaggaaaa
     3781 tatttatcta aagaattttc tttatttaac gaagttgcag aagaaaaaaa taatctgact
     3841 tttaattctg tacattatga tttatatact aatatcaatt ataatattgt cataagatat
     3901 aatgagatac cggagttttc taaccattac cttagaaatg tttcatataa catgttgaca
     3961 ttttatattc tgggaatagg aacgagtatc agcattgtag ttgctttaac acggtttgta
     4021 aaagaaattt ctttgaattt caaggaaatc aagaagttag caaataaaat ggggatagaa
     4081 gtcctttctg aaaatgaaaa ttattctaaa ataatcgagt ttgatgatat acttagaact
     4141 ttacatatta aaggtgataa tttgaaaagt ttgattgagc gagaaatatt ggaaaagcaa
     4201 gatctatcct ttcaaattgc agcactttcg catgatataa aaacaccttt aacggttttg
     4261 aaaggaaatc ttgagttatt agaattgact actttaaata aaaatcagga aggttatatt
     4321 gtatctatga acaatagtat ttctgttttt gaaggctatt tcaattcttt aatatcttat
     4381 acacgcatgc tttcagagga tagatcagtg aaactaattt tagttgagga gctattaagt
     4441 gagttacatt ttgaagtcga tgacttattg aatatcaata atattgaatt ttctatttgt
     4501 aatagactta ttatcacttc tttttatgga gatgaggaaa atctgataag agctctttct
     4561 aaccttttag ttaatgcaat aagatttatg cccgtattag ataaaaaaat cgaagttata
     4621 ctgtctgagt caggagagca aattcatttt gaaatatgga ataatggtga gcgtttcagt
     4681 gatagtacac tgaaaaaggg agataaatta ttttacacgg aagattatag cagaggtaac
     4741 aaacattatg gaatcggttt ggcatttgtt aaaggcgttg ctatcaaaca cggtgggaat
     4801 cttcaattga ataatccagc tagaggaggt gctagcgcca taatctcgat aaaaaagaaa
     4861 atttaataga tttgattgaa actggagtat ttcaggcaaa aaatcgtaaa ttttggtgat
     4921 ttttggctaa aatacttttt ttctgaacaa attctgaact tggggtgtca taataatcat
     4981 gtaaatatat cggatgcaaa aacgtaagag aggaactaga aaatgactaa cacaattaaa
     5041 gactttgatc tcgatttgaa aacaaataaa aaagacactg ctacacctta tgttggtagc
     5101 cgttacctat gtacccctgg ttcttgttgg aaattagttt gctttacaac aactgttaaa
     5161 taataaagtt atcattatca tcaacattat aatttcaaat aatttgatat atttataaat
     5221 ttaggggagt agactgattt tagtttgctc ctttattagt agataaggaa gttatacatg
     5281 attaaagctc agctaaagaa tctaataaaa acaattcatc tcgtaaaaga ggctggtttc
     5341 tccctactgg tgttctcaat tgctattagc ttatttcaag ggattttacc catttttagt
     5401 atgctactag ttcaaaaaat gttaaacatt ataacgactg atataaaaaa ttttcacaca
     5461 ttgatgattg cttttatctc atatattgct ttaacattat tgacaattat aataggagaa
     5521 gttgatagtt atattgatac taaattacag atacttcttc actataagat gaaccattta
     5581 gttatgcaga aaactgtaaa gttaacattg gctgaatttg agacaccaga aatctatgat
     5641 gatatcactc gcatacaaaa tcaaatatct tataaacctt ttcaaatata taagtcaatt
     5701 atttctgtat tatcttcgtt agtatcatta atttcctcat ttgttatttt attaaattgg
     5761 aagatatcaa tttttccact tttacttatc cttcctatcg tttctatata tatctacttg
     5821 aaaattggta aaaatgaatt tgagatacta tataaaagga gtagcgatga gagagcaaat
     5881 tggtatatta gtcatctttt aacacatgat tttgctgtca aggagttgag gttaggtacg
     5941 ttagaaagtt attttctaaa aaaatatgat aaaaataatc aattatttat taatcaggaa
     6001 aatagtataa atagatctaa aatgaaatac ggtatagtat taggtgtatt ggagattgct
     6061 atacaatcta ttattatgtt ccttgcagtt agagaagcat ttctaggaat attattagtt
     6121 ggaaatgtaa ctacttttat aaggtcttta tctacaatgc aaacaagtac acaaaatata
     6181 gtaaataata tctattcttt ctataatggt tcattgtaca tggagttatt gtatgatttt
     6241 acgcatgatg agttagtaca taaccctata atcgaaagta cggttgatac aattcagtca
     6301 gttgaatttg aaagagtttc tttttcatat aatggtaagg ataatgtaat aaacgatata
     6361 agtttttcaa ttaaagcagg agagaggtta gcaattgttg gtgaaaatgg ttctagaaaa
     6421 agtacgattt tcaaattagc ctgcggtctt tacgataatt atgaaggcaa tatatatata
     6481 aatggtatta atcttcgttc aatacagaaa aagtcatact acaagagaat atccgcattg
     6541 ttccaagatt ttttaaagta tgaattgaca ctgcgtgaaa atgttggatt gggtgaatta
     6601 tcaaagttat atagtgatga ggatttaata agtgcactga agtatacagg agttgattcg
     6661 atattttact ttaatcagga aaaattaaat cagatagatc tggaacagca attagggaat
     6721 tggtttgagg atgggagaca gttatctggt ggacagtggc aaaaaattgc attatcaaga
     6781 gtatatctca aagaagcgga ttgctatttg ctagatgagc ctagttctgc gttagatcct
     6841 gagtctgaaa caaaaatttt taaaacattt tttgaattat cgcaaaataa aattggtatt
     6901 tttattacgc ataagccaag cattactcaa tctgtgaata aaattttgta tctagataat
     6961 ggatgtatat ctgaaacagg aagtttcgct gatttaaata ttaaaggaat gaagtttaaa
     7021 aacttattag ataaagaaat tggagccaca tatgacacct caaattaaac agatagtaga
     7081 ttacattgca gatagtttta taaattatga acagatagta gctgaaagtg agaataaagt
     7141 actaattaat attgaatcta aatatagaaa tacctttttt gataaatctc tgagtcatgg
     7201 tgtgccatca ttaatcttaa tgtatagttc actatataaa gtgacaaaag atgagagata
     7261 tctaacattc tccaacatat acgttgagaa attagtagac ataatctcta gagatggtat
     7321 agaatctccg tctctctatg ctggcactgc aggtattgca ttagctatta aagaagcgag
     7381 tatatctgga aagtattatc cgaaattatt atcatcctta aattgtcttc taaaagaaca
     7441 aattaaagac aaattggtag taagtttttc taatattgag aaaggaatta ttgagccata
     7501 tgattatgat atggttaacg gatttagtgg aataactaat tacttactcc ttgaacaaga
     7561 atttttttct gtagagttaa atcaaattgg aaattatctt ttgaaatata ttgagacaat
     7621 tttaaataag gtaactaatt gttctgaaga tagagaagct gaatttgatc taggaattgc
     7681 acatggaatt actggtccta tgctaatcct agcaaaatta aaatcagaaa aaattttgag
     7741 tgtagaagta ggagatatac tgaataaagc tataaattta atgtttttat ttagaaggga
     7801 tgacaaacta tggcctggaa aaatttatac tagtaatatt ttggatcttg atttaaatta
     7861 tcttcctact agaatggcat ggtgttacgg tatgcctgga actgcagttg ccctttttaa
     7921 aatgtgtcaa ttgatagatt taaattttat tgacgatatt aatgaaagtt taattgctca
     7981 gatgagaagt tcagacaaaa atgtctattc tcttacaatg tgccatggtt tggcaggagt
     8041 aacttttata tatgacttga ttggtaaaaa ttattcaagt gagaggctaa ctcgctttgc
     8101 agaagagctt cgccaacaaa taattacatc gttttcaact acaaaagtat ttgggtatac
     8161 tgacagagaa aaatttggag aaatagaagt tgaactggag tcaataggga tattgcaagg
     8221 ggtatcaggg atagtgttat tcttattaaa tccttacagt gacgaggagc tattgtggca
     8281 aagaatattt atttaatttg aaaaggagaa caaaatgtat aaggtatttc caactggtat
     8341 gattcgtatt ccattgcaaa aacaagaatt ttataataaa gactatgaag atttaataaa
     8401 tgataaagtt tttatggaac aacttttagt tgctagtccc agtctttatg atagtgtaat
     8461 agattttgat aaatgtaatg aaaagaaaaa gcaaaatgta aaagtctcat taaatagtta
     8521 ttctaaaaga gcagcgttta gaactactcc atttggtatc tttactgcta ttaatacagt
     8581 tgatttgaca aagggaacca cttctaatgt acaaaaggtt agttttatta aaaaagcagt
     8641 cccagattat tcatggatat attctctagt aaaatcgtat gaaataagta accttgaaaa
     8701 actttcatta aaaataaata cagcggcgtt tactcaaggt gacagatatg tactaccatt
     8761 tacagtgaat gaatcagagg aagatagaaa cataagtttt tcaaaaccaa ttaagttact
     8821 tgtagaaaaa tgtaaaactc gatatataaa atatgaagag ttaatagatg tatttaaaaa
     8881 taattatcca gatatagtgt cagacatgct agagtcatat atacacgact tagttacaaa
     8941 tgatttttta atttcagatt taagaccacc aatttgtaat atcaattcct tagattatct
     9001 actatctaaa ttagaagaag gaacactatg tactgactta actactttaa agaagatgat
     9061 tgaagattac aacgatttaa atattgggga aggtagagac ttacttttgc agatttttga
     9121 taaaatggaa agtatttata aatatgataa cggtggaaat tacttaaaag ttgattcatt
     9181 agcagagtat gaaggcgatt ttaatattct atctgaaacg gctcgggaaa gtatagaacg
     9241 actcgcagag atgtttgtaa aattaaatac aaatgtaaag agaagagggg ggaggaaaaa
     9301 ttcacttgaa gagtatcagt tgaaatttat tggcaaatat ggagaaaact gctcaattcc
     9361 ctttgttgaa gtaataaata aagatgcagg cataggtttt cctgaatatt acaaaggagg
     9421 agaaggtgaa gcaatagagt taagcgatcc aataatgcaa atgtttgaaa agaaatatga
     9481 ggaagctcta ttaaatggag ggcatatcga attttattca aaagatttag atgattttca
     9541 aacagatcaa agcaatagtt tagattcgtt tgaattaaat tttaatataa aaataataaa
     9601 taatgatgtg aagttatatt taggagctaa tatcggttct ggacaagcag gtagaagctt
     9661 tggaagattc tatggattaa gcgaaacagt aagagaaaca attaagaatt taaaccatca
     9721 aaataatacg aatgtagagt tgtcatttgt tcctaaacag ataagacttg cgaatgtaat
     9781 tcaaaattat agtgatgagt cttataatac ttcagttttt accacatcat gggattcaga
     9841 gaacgaactg cgattggagg atatttatat aagatatagt gatggtaagt ttcattttac
     9901 aactatcgat gggaaaaatg agttgaaatt taccatgaat aatatgctaa attccgatag
     9961 ccaaagcagg gttttgagat tgttggttga tttatctgag tttgagtacg gtttatcgca
    10021 ttggtcatta tttccttggg atattttggc tcaggaaaga gtatatattc ctgaaatttt
    10081 atttgaagac attccgattg ccactgctca gtggaattta tcagtaatta gagaagagat
    10141 tatacatcaa aaaatattta ctggtaaaaa agatatattc aataagttta gaaagactta
    10201 taagatacct aaagatatta ttttgaaata tgcagataat gaattgaggt tagatttatc
    10261 tatagatagt gatttagaga ttttatttaa aaacattaat aaatataggt ttgtttcttt
    10321 gagaggaatt gagcagggag agtctatttt taaggataat ttagggaact acaatacaga
    10381 gatagtagtt ccaattctta aggaatctaa tggaataaac aatacaaacg ataatgttca
    10441 atttaaaaat gaagttaaga aaatatattt accattttgt gactggctat ttttcaaatt
    10501 ttatggtcca aaagagcgac aagaagaatt actaaataac tggaaaaaat tttggctaaa
    10561 cctgccatca gaactgttta gccaaagtaa catgttttat atgagatata atgataccaa
    10621 tgatcatata agaattcgga taaattgtga ttcgattgaa aataactttt ctttgtattt
    10681 gtcagtagtt caagatctat tacctcaact gattgagagt tgtattatct ctgacataga
    10741 agtgagtagc tataaaccag aagtaaatag atatggagga cctcatttaa taagttatgc
    10801 agaggaaatt ttttgtaaag aatctatctt atttcttaat aatacaatag aattttcaga
    10861 tgatgaaaga ttagtttgtg ctac
//
LOCUS       DAUCLR010000063        10623 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.063, whole genome shotgun sequence.
ACCESSION   DAUCLR010000063 DAUCLR010000000
VERSION     DAUCLR010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10623
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.063"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..90
                     /locus_tag="VGT87_001356"
     CDS             <1..90
                     /locus_tag="VGT87_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain protein"
                     /protein_id="HEQ3814200.1"
                     /translation="AKRAAKRLKTKMKIVPITTVQEALVYLRK"
     gene            504..1481
                     /locus_tag="VGT87_001357"
     CDS             504..1481
                     /locus_tag="VGT87_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEQ3814201.1"
                     /translation="MVTIKQVAEEAGVSRSTVSRYISQKGYVSDDARHKIKAAIAKLH
                     YTPNVLAQSLKTKKNQLVGLLLPDISNPFFPRLARGAEEYLKEKGYRVMLGNISDSEA
                     LEEEYVHVLLQSNAAGIITTHDFTKRYPTLAIPVVVVDRVDQETQYGVFSDNRAGGLL
                     AAQTVWQAGAKEVLLIRGPLDNAENINERFEASFSYLQKQDVTMYVCDSQNFDFESIQ
                     LEASYNLKCYPTIDSIIAPSDIHAIAYIHELHSQGKKIPQDVQIIGYDDILMSQFIYP
                     SLSTIHQSSYLMGRYAAELVYTIASQLTVKANRIKLPVHYVERETIRKR"
     gene            1905..2549
                     /locus_tag="VGT87_001358"
     CDS             1905..2549
                     /locus_tag="VGT87_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1027 domain-containing protein"
                     /protein_id="HEQ3814202.1"
                     /translation="MDFSERCQMKKEISPEMYNYNKFPGPKFIHFEEQVKAEGIDLLL
                     LEDVKNAFDTTSFGQRYTEVLLKYDYIVGDWGNEQLRLKGFYKDSDDIKKTNRISRLE
                     DYIKEFCNFGCAYFVLENLHPQDIKFEEERQPRRKKSPKSKSNRRKPNYSNQQPAIPK
                     SKSKRVSKEKQPENQAFTSQKRRSNTKHKEKSKRNQTSQLNTKISHFIIRKKDK"
     gene            2553..3632
                     /gene="rlmN"
                     /locus_tag="VGT87_001359"
     CDS             2553..3632
                     /gene="rlmN"
                     /locus_tag="VGT87_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEQ3814203.1"
                     /translation="MKPSIYSLTRDELIAWAVERGQKQFRATQIWDWLYKKRVQSFEE
                     MTNISKDFVSILNDSFCVNPLKQRVVQESADGTVKYLFELPDGMLIETVLMRQHYGHS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNSGEITAQIMLVQKYFDDRKQGERVSHVVVM
                     GIGEPFDNYKNVMCFLRVINDDNGLAIGARHITVSTSGLAHKIRDFANEGVQVNLAVS
                     LHAPNNDLRSSIMRVNRSFPLEKLFSAIEYYIEKTNRRVTFEYIMLNEVNDSIKQAQE
                     LADLTKTIRKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDVLKKNGVNCVVRQEHGT
                     DIDAACGQLRSKTMKKDREKVTATK"
     gene            complement(3714..4340)
                     /locus_tag="VGT87_001360"
     CDS             complement(3714..4340)
                     /locus_tag="VGT87_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEQ3814204.1"
                     /translation="MIAFFSICSGILLTHLFYCLLLYFPDNGQEQLSKQTVIVCDQLT
                     FAKRVFRQLQNHFITSQKIFFSLEISLIIILWASMVHWVSASYCYLLLFSLLFSLFDW
                     RSQEYPFILWLFSFVSLLLFYSINYLSLILLLLGLLAHLRPFSIGAGDFFYLASLALV
                     LDLTSLIWLIQLASLAGITACLLLGIKRIPFIPYLSFGLFWIVLLEHF"
     gene            4568..5095
                     /locus_tag="VGT87_001361"
     CDS             4568..5095
                     /locus_tag="VGT87_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEQ3814205.1"
                     /translation="MTNTLVENIYASVTHNISKKEASKNEKTKAVLNQAVADLSVAAS
                     IVHQVHWYMRGPGFLYLHPKMDELLDSLNANLDEMSERLITIGGAPYSTLAEFSKHSK
                     LDEAKGTYDKTVAQHLARLVEVYLYLSSLYQVGLDITDEEGDAGTNDLFTAAKTEAEK
                     TIWMLQAERGQGPAL"
     gene            5237..5440
                     /locus_tag="VGT87_001362"
     CDS             5237..5440
                     /locus_tag="VGT87_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEQ3814206.1"
                     /translation="MKTLYDVQQLLKNFGIFVYLGKRLYDIEMMKIELQRLYDSGLLD
                     KRDYLNAELILRREHRLELEKEG"
     gene            5445..6416
                     /locus_tag="VGT87_001363"
     CDS             5445..6416
                     /locus_tag="VGT87_001363"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEQ3814207.1"
                     /translation="MSQKLLGIDLGGTTIKFGILTAAGEVQEKWAIETNILEGGKHIV
                     PDIIASIKHRLDLYGLSSADFVGIGMGSPGAVDRDTNTVTGAFNLNWKETQEVGSVVE
                     KELGIPFAIDNDANVAALGERWVGAGENNPDVVFMTLGTGVGGGIIADGNLIHGVAGA
                     GGEIGHMIVEPENGFACTCGSHGCLETVASATGVVKVARLLAEAYEGDSAIKAAIDNG
                     EGVTSKDIFMAAEAGDSFAYSVVEKVGYYLGLASANISNILNPDSVVIGGGVSAAGEF
                     LRSRIEKYFVTFTFPQVRYSTKIKIAELGNDAGIIGAASLARQFITK"
     gene            6426..6815
                     /locus_tag="VGT87_001364"
     CDS             6426..6815
                     /locus_tag="VGT87_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEQ3814208.1"
                     /translation="MSPITLILWLLLVGIVGYYTWNYFSFRKMAKQVDNETFKDVMRQ
                     GQLIDLREPAAFRTKHILGARNFPAQQFDAAIKGLRKDKPVLIYENMHPQYRVPAVKK
                     LKKAGFEDVYVLKDGIDYWDGKVKQTT"
     gene            6998..8839
                     /gene="typA"
                     /locus_tag="VGT87_001365"
     CDS             6998..8839
                     /gene="typA"
                     /locus_tag="VGT87_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEQ3814209.1"
                     /translation="MTNLRNDIRNVAIIAHVDHGKTTLVDELLKQSHTLDERKELQER
                     AMDSNDLEKERGITILAKNTAVAYNDVRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLIPIVVVNKIDKPSARPAEVVDEVLELFIELGADDEQ
                     LEFPVVYASAINGTSSLSDDPADQEHTMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDCVEALPILRIDEPTLQMTFLVNNSPFAGREGKWITSR
                     KVEERLLAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IIKEIDGVKCEPFERVQIDTPEEYQGAIIQSLSERKGDMLDMQMVGNGQTRLIFLIPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPVVQGEIGGRHRGALVSIENGKATTYSIMR
                     IEERGTIFVNPGTEVYEGMIVGENSRDNDLGVNITTAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAARDKANKKKKSAE"
     gene            8961..9212
                     /locus_tag="VGT87_001366"
     CDS             8961..9212
                     /locus_tag="VGT87_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEQ3814210.1"
                     /translation="MFYLIMAVLIISYYLYMAPKSVRNTLGMIGLVGLVALLIVLAGL
                     SFIKIMQTPPEFFIGMGMVALGYFALKDVRKMTKKPRVK"
     gene            9568..>10623
                     /locus_tag="VGT87_001367"
     CDS             9568..>10623
                     /locus_tag="VGT87_001367"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEQ3814211.1"
                     /translation="MKVISNFQNKKILILGLAKSGEAAAKLLTKLGALVTVNDSKPFD
                     QNPAAQALLEEGIKVICGSHPVELLDEDFEYMVKNPGIPYDNPMVKRALAKEIPILTE
                     VELAYFVSEAPIIGITGSNGKTTTTTMIADVLNAGGQSALLSGNIGYPASKVVQKAIA
                     GDTLVMELSSFQLVGVNAFRPHIAVITNLMPTHLDYHGSFEDYVAAKWMIQAQMTESD
                     YLILNANQEISATLAKTTKATVIPFSTQKVVDGAYLKDGILYFKEQAIIAATDLGVPG
                     RHNIENALATIAVAKLSGIADDIIAQCLSHFGGVKHRLQRVGQIKDITFYNDSKSTNI
                     LATQKALSGFDNSRLILI"
BASE COUNT         3506 a         1807 c         2149 g         3161 t
ORIGIN      
        1 gccaaacggg cagccaaacg actaaagacc aaaatgaaga ttgttcctat tacgactgtt
       61 caagaggcac tggtttatct tcgcaaataa aataactatg aatgtattat attcaattat
      121 attcacgcta ttaactcata aaaaacggtt gaaccagata gacagatcct tgaccagtct
      181 tttgggttca atcatttttt acataaaaca tatctaactt gacagcgtaa aaaagatgtt
      241 ataaaagtct agtggtaaaa aggcaataga gaattgagta aactttagtc gattatctgg
      301 aaatagagca tactaaaaac acctagtgaa aattgatttt tttcactagc tcatccaaga
      361 acttgcgcag ataatcatga gtccattcca tgactctatt attctggaaa gccttgcaat
      421 agacaagtta acgttataac aactaaatta aaaacttgag attgaaaaat ccccataaac
      481 ttattttaca aggaggtaag gctatggtaa ccattaaaca agttgctgaa gaagcaggtg
      541 tttctagatc aacagtctcc aggtatattt ctcaaaaagg atatgtatct gatgatgcaa
      601 ggcataaaat caaagcagcg atagcaaaat tacattacac tccaaatgtc ttggcgcagt
      661 cattgaagac gaaaaaaaat caactagtcg gtttattatt accagacatt tccaatcctt
      721 tttttccgag gttagcaagg ggagcagaag aatatttaaa agaaaagggg tatcgggtta
      781 tgctaggaaa tatttcagac agcgaagctc ttgaagaaga atacgtccat gtccttcttc
      841 aaagcaatgc tgctggcatt attactaccc atgactttac aaagcggtat cctactcttg
      901 ctattccagt tgtagtggta gatcgtgttg accaagagac ccagtacggt gtcttttcag
      961 ataatagggc agggggctta ttggcggctc agactgtctg gcaagcaggg gcaaaagaag
     1021 ttttgttaat aagaggtccg ctggataatg ctgaaaacat taatgagcgg tttgaagcta
     1081 gtttttctta ccttcaaaaa caagacgtga ctatgtatgt gtgtgatagt cagaattttg
     1141 attttgaaag tattcagcta gaagctagtt acaacctcaa gtgttaccca acaatagaca
     1201 gtattatagc accttctgat atccatgcta ttgcttatat tcatgaactg cattctcaag
     1261 gtaaaaagat tcctcaagat gttcaaataa taggttatga tgacatttta atgagtcaat
     1321 ttatttatcc ttctttatcg actattcatc aatcctctta tttgatgggc cgttatgccg
     1381 ccgaattagt ttatactatt gctagtcaat taactgtgaa agcaaatcgg attaaattgc
     1441 ctgttcacta cgttgagcga gaaacaataa ggaaaagata agtaataata ttcgtccaga
     1501 ttttagcagg tatgatgaag atttgttggg atttaccttc tactttaaaa gatgttatct
     1561 gtcattactt tcatcgctat cagtcaaaac gcattcttat gcaactctta accttaagct
     1621 taaggtcttt ttgataacat agaggaatgt tacacgatga tgacgcaata aagcctaaat
     1681 atagtaattg tcgtgatttt atagctagtg agtaatgaga tagcaagtct ctcatttact
     1741 tacaaaagtc aaaaaatagt gtataatggt tgagttacag ataacactgc aaaaaagata
     1801 acaagtgtcc ataattatct aagactataa aggcgatata gacagatata aaaaagatga
     1861 cgaatcagtt atcactggac aattaaccga tttagttaca actaatggat ttttcagaaa
     1921 ggtgtcaaat gaaaaaagaa atttcgccag aaatgtacaa ctataataaa tttccaggtc
     1981 ccaaatttat tcattttgaa gagcaagtta aggctgaagg cattgatttg ttactcttag
     2041 aagatgttaa gaacgctttt gatacgacta gctttggtca acgttataca gaagttctcc
     2101 taaagtatga ctatattgtt ggcgattggg gaaatgaaca gcttcgtctt aaagggtttt
     2161 ataaggatag tgacgatatc aagaaaacga atcgcatctc acgtttagaa gattatatta
     2221 aagaattttg caattttggt tgtgcttatt ttgttttaga aaatcttcat ccacaagata
     2281 ttaaatttga agaggagcgc caaccaagac gaaagaaatc acctaaatca aaatcaaatc
     2341 gtcgcaagcc aaactattca aatcagcagc ccgcaatacc taagagtaaa tcgaagcggg
     2401 tatcaaaaga aaagcaacct gaaaaccaag cgtttaccag tcaaaaacgt cgaagtaata
     2461 ctaagcataa agaaaagtca aaacgtaatc agactagtca acttaatacc aaaattagtc
     2521 attttatcat cagaaagaaa gataaataat ccatgaaacc atcgatttat agcttaacac
     2581 gcgatgaact gatcgcgtgg gcagttgaac gcggccaaaa acagtttaga gctactcaaa
     2641 tttgggattg gctctataaa aaaagagttc aatcttttga ggagatgacg aatatttcta
     2701 aagattttgt ttctatttta aatgatagtt tctgtgtcaa tcctttaaaa cagcgagttg
     2761 tacaagaatc agctgatggt acagtaaaat atttatttga actgcccgat ggtatgttaa
     2821 ttgaaacagt tttaatgcgt caacattatg gtcattctgt ttgtgtgacg actcaagttg
     2881 gctgtaacat tggctgtact ttttgtgcta gtggattgat caaaaagcaa cgtgatctaa
     2941 acagtgggga aattacagct caaatcatgt tagtccaaaa atattttgac gatagaaagc
     3001 aaggtgagcg ggttagccat gttgtcgtaa tgggcattgg tgaacctttt gataattaca
     3061 aaaatgtcat gtgctttctt cgcgtcatca acgatgataa tggcttagca attggtgccc
     3121 gtcatattac agtctctacc tcagggttag ctcacaagat tcgggatttt gcaaacgagg
     3181 gagtacaggt taatttagca gtctcgttgc atgcaccaaa taatgattta cgttcaagta
     3241 ttatgcgagt aaatcgctct tttccgctgg aaaagctctt ttcagctata gaatactata
     3301 ttgaaaaaac gaatcgtcgt gtcacttttg aatacattat gctaaatgaa gttaacgata
     3361 gcattaagca agctcaagaa ctagcagact taactaaaac tattcgtaag ttatcttatg
     3421 ttaacttgat tccgtataat cctgtttcag aacatgatca gtatagtaga agtcctaaag
     3481 agcgagttct agcgttttat gatgtgctaa agaaaaatgg agttaactgt gtggtgcgtc
     3541 aagagcacgg aacagatatt gatgcagcct gtggccagtt acgttctaaa acgatgaaaa
     3601 aagatcgcga aaaggttaca gcgacaaaat aaagtccaaa aaatatcttt gattgacaat
     3661 gcttgatgac aaaatattcc tagttactaa aatgactagg ttttttatct ggcctaaaaa
     3721 tgctctagaa gcacaatcca aaataatcca aaactaagat aaggaataaa aggtatgcgc
     3781 ttgatgccta atagtaagca agcagtaata cctgctaaac tagcaagttg aattagccaa
     3841 atcaacgacg ttagatccaa tactaaagct aagctagcaa ggtagaaaaa atcgcctgct
     3901 ccaattgaaa aaggtcttaa atgagctaat aaacctagta gcagtaatat caatgataaa
     3961 taattgatcg aataaaacag gagtagactt acaaagctaa aaagccacaa gataaaaggg
     4021 tattcttgag aacgccaatc aaataaacta aataataaag aaaagagtaa caagtaacag
     4081 taactagcac taacccaatg taccatacta gcccataaga taattattag acttatctct
     4141 aagctaaaga agattttttg actagttata aaatggtttt ggagctgccg aaaaacgcgt
     4201 ttggcaaaag ttagttggtc acaaacaatg acagtctgtt tacttagctg ttcttgacca
     4261 ttatcaggaa aataaagtag taaacagtaa aaaagatggg tcaataagat gcccgaacat
     4321 atactaaaaa aagctatcat acttatttat tcgaaaagaa atcaaatact tgcaatcttt
     4381 ccttaatcat tgtagaatat agatataaaa cgataatgat tctaattaag tgtctaatca
     4441 tccaattagg attattccgt tattgtcgaa taggattagg tccttaaccc tttcttatgt
     4501 ttatcacgtt agggttagtt ccccctccat tcttgccctt taagctgaaa agtaaggaga
     4561 tgttattatg acaaacacac tcgttgaaaa tatttatgct tcagtaactc acaatatttc
     4621 taaaaaagaa gcatcaaaaa atgaaaaaac taaagctgtt ttaaaccaag ctgtcgctga
     4681 cttatctgtg gctgcatcta ttgtgcacca agtacactgg tatatgcgtg gtccaggttt
     4741 cttgtacctt caccccaaaa tggatgaatt acttgatagc ttgaatgcta atttagatga
     4801 aatgagcgag cgtttgatca ctattggcgg agcgccttat tctaccctag ctgagttttc
     4861 taaacactct aaattggacg aagcaaaagg tacttacgat aaaacagttg ctcagcattt
     4921 agcaagattg gtcgaagttt acctttacct cagcagcctt taccaagttg gtttggatat
     4981 taccgatgaa gaaggcgatg ctggaacaaa cgatttgttt acagcagcta agacagaggc
     5041 tgaaaaaacc atttggatgc tccaagcaga gcgtggacaa ggcccagcac tctaattgat
     5101 tatgccttag gataaaagaa gtcaatgata aaattggctt cttttttatg gaatcatcga
     5161 ttcattttca gaaacataga aagcgattcc ataattgtct tgaaatctgc cgtagaattt
     5221 gctaaaatct acttatatga aaacactata tgatgtgcaa caattgctaa aaaactttgg
     5281 catttttgtt taccttggca aacgtcttta tgatattgaa atgatgaaga ttgaacttca
     5341 gcgtctctat gatagcggct tattggataa gagggattac ttaaatgcgg agttaatttt
     5401 gcgacgtgag catagattag aattggaaaa agaaggatag acaaatgagt caaaaattac
     5461 tggggattga tttaggtggt accaccatta aatttggaat tttaacagca gcaggagaag
     5521 tccaagaaaa atgggcaatt gagactaata ttttagaagg aggcaaacat attgttcctg
     5581 atatcatcgc ttccatcaaa catcgcttag acttgtatgg tctaagcagt gctgattttg
     5641 tgggaattgg tatgggatca ccaggagcgg ttgaccgtga tactaataca gttactgggg
     5701 cattcaacct taattggaaa gaaacccaag aagtcggttc cgttgttgaa aaagaattgg
     5761 gcattccttt tgccattgac aatgatgcca atgtggctgc ccttggtgaa cgttgggtag
     5821 gtgctggtga aaataaccca gatgtcgtct tcatgacact tggaacaggt gtcggtggag
     5881 gcattattgc tgatggtaac ttgattcatg gtgttgcagg agcaggtggt gaaatcggcc
     5941 acatgattgt tgagccagaa aatggctttg cttgtacttg tggctcacac ggctgtttgg
     6001 aaacagtagc ttcagcaaca ggagttgtca aagtggcacg tttactggca gaagcctacg
     6061 aaggggattc agccatcaaa gcagctattg acaatggtga aggtgttacc agtaaagaca
     6121 ttttcatggc ggctgaagca ggggattcct ttgcttattc tgttgtggaa aaggttggtt
     6181 actaccttgg ccttgcttca gcaaatattt ccaatatctt gaatccagac tcagtggtta
     6241 tcggtggggg tgtgtcagca gcaggagaat ttctacgctc acgtattgaa aaatactttg
     6301 tgaccttcac tttcccacaa gtgaggtatt caactaaaat taagattgca gagcttggca
     6361 atgatgctgg aattatcggt gcagctagcc ttgctcgaca atttatcaca aaataggagg
     6421 gggatatgtc tccaatcaca cttatcttat ggttacttct tgtcggcatt gttggttatt
     6481 atacatggaa ctatttctct tttcgaaaaa tggcaaagca ggttgataac gagactttta
     6541 aagacgtgat gcgtcaaggt caattgattg atttacgaga gccagcggct tttagaacta
     6601 agcatatttt aggagctcga aattttccag cgcaacaatt tgatgctgct attaagggac
     6661 tacgcaagga caaacctgtt cttatttacg aaaatatgca tccccagtac cgtgtgcctg
     6721 cggtcaaaaa acttaaaaaa gctggctttg aagacgttta tgtcttaaaa gacggtattg
     6781 attattggga tggcaaagtt aaacaaacaa cctagttaag agtaacgtgg agttaagggg
     6841 aataaagaca gtcactgtct caaaaacctt aattcctttt tttgctgtat ccagacttgc
     6901 tgaaagtctg aaaacattta caattgatta aaaccagttt tttaaaacat tttgtgttat
     6961 acttatctag ttaaaatata tttacttaga ggaacaaatg actaacttaa gaaacgatat
     7021 ccgtaacgta gcgattattg cccacgttga ccacggaaaa acaacacttg tagatgaatt
     7081 attaaaacaa tcccatactc ttgatgagcg taaagagctc caagagcgtg ccatggattc
     7141 caatgacctt gaaaaagaac gtgggattac aatccttgcg aaaaatacgg cagtagccta
     7201 taacgatgtt cgtattaaca tcatggatac cccaggacac gcggactttg gtggtgaagt
     7261 tgaacgtatc atgaaaatgg ttgacggggt tgttcttgtt gtggatgcct acgaaggaac
     7321 aatgccccag acgcgtttcg tattgaaaaa agcacttgag caaaacctta tcccaatcgt
     7381 tgtggtgaac aagattgaca aaccttcagc tcgtccagca gaagttgtag atgaagtgct
     7441 tgaattattc atcgaacttg gtgccgatga tgagcaattg gaattcccag ttgtttacgc
     7501 atcagctatt aatggcacat catcattatc agatgatcct gctgaccaag agcatactat
     7561 ggcacctatc tttgatacga ttattgatca tattccagcg ccagttgata attcagatga
     7621 gcctttgcaa ttccaagtgt cacttttgga ctacaacgat ttcgtaggtc gtatcggtat
     7681 cggtcgtgtt ttccgtggta ctgttaaagt tggtgaccaa gtaactcttt caaaacttga
     7741 tggtaccact aaaaacttcc gtgttacaaa actgtttggt ttcttcggtt tggaacgtcg
     7801 tgaaattcaa gaagctaaag caggtgactt gattgctgtt tcaggtatgg aagatatctt
     7861 tgttggagaa accattacac caactgactg tgtggaagct ctgccaattc ttcgtattga
     7921 tgagccaacg cttcagatga ctttcttggt caataactct ccttttgcag gtcgtgaagg
     7981 taaatggatc acgtcacgta aggttgaaga acgtctttta gcagaattgc aaacagacgt
     8041 gtcacttcgt gttgacccaa cagattcgcc agataagtgg acggtttcag ggcgtggaga
     8101 attgcacttg tcaattctta tcgaaaccat gcgccgtgaa ggctatgaac ttcaagtatc
     8161 acgtccagaa gttatcatca aagaaattga tggtgtcaaa tgtgaaccgt ttgagcgtgt
     8221 tcaaattgat acaccagaag aatatcaggg tgcaatcatc cagtctcttt cagaacgtaa
     8281 aggggatatg cttgatatgc agatggttgg taatggtcaa acgcgtttga ttttcttgat
     8341 tcctgcacgt ggtttgattg gttattcaac agagtttctt tcaatgacac gtggatatgg
     8401 tatcatgaat catacttttg atcagtatct accggttgtt caaggagaaa ttggtggtcg
     8461 tcatcgtggt gccttggttt ctattgaaaa tgggaaagca acgacatatt caatcatgcg
     8521 tattgaagaa cgtggaacta tctttgtcaa cccaggtaca gaagtttacg aagggatgat
     8581 tgttggtgaa aactcacgtg ataacgacct tggtgttaac attacaacag ctaagcaaat
     8641 gacaaatgtc cgttcagcga ctaaagatca aactgcagtt atcaagacgc ctcgtatcct
     8701 aacgcttgaa gaatcattag agttcttgaa tgacgacgaa tacatggaag taacgccaga
     8761 atctatccgc ttgcgtaaac aaatcttgaa taaagctgcg cgtgataaag ctaataaaaa
     8821 gaaaaaatca gccgaatagc ttttgaagtt aaactaacta aataaaatag caaaaagata
     8881 tttaagaaaa gtcatttttt aaaggtcttt ttgcgtttac aagttcctat ggaatataaa
     8941 ccaaaataaa ggaggacgga atgttttacc ttattatggc tgtcttgatt atatcttatt
     9001 atctatacat ggctcctaaa agtgtccgta acacccttgg gatgattggt ttagttggct
     9061 tggttgctct gcttattgtt ttagcgggct tgagttttat caaaatcatg caaacccctc
     9121 ctgaattttt tattgggatg ggaatggtgg cattgggcta ttttgcttta aaagatgtgc
     9181 gtaaaatgac caaaaaaccg agagttaaat aaaagtactt aaagattgat gattttggtt
     9241 tttatacgat aaaaggactg atttgtttta gacaatatca gtcctttttt aatagttcat
     9301 tttttctgaa agaaagcttt tagccgattt tcattgagtg tgcaactaaa aagagatgtt
     9361 tggtaataat agttaagacc tctgactagt attttatctt attttactta aaaaaacgta
     9421 ccaataatct cacaaatatg ggcatgatgt ttgtatattg gaaacggatt ctaattctca
     9481 actcaagaac ggtattttta aaatgttgaa aaagcatcta aataaaggta aaatagaaat
     9541 caaatagaat ggatagtgtg agcttagatg aaagtgataa gtaattttca aaacaaaaaa
     9601 atattaatat tggggttagc caaatcaggc gaagcagcag caaaattatt gactaaactt
     9661 ggtgctttag tgactgttaa tgatagtaaa ccatttgacc aaaatccagc ggcacaagcc
     9721 ttgttggaag aggggattaa ggtcatttgt ggtagccacc cagtagaatt attagatgag
     9781 gactttgagt acatggttaa aaaccctggg attccttatg ataatcctat ggttaaacgc
     9841 gcccttgcaa aggaaattcc catcttgact gaagtagaat tggcttattt cgtatctgaa
     9901 gcccctatta tcgggattac aggatcaaac gggaagacaa ccacaacgac aatgattgcc
     9961 gatgttttga atgctggcgg gcaatctgca ctcttatctg gaaacattgg ttatcctgct
    10021 tcaaaagttg ttcaaaaagc aattgctggt gatactttgg tgatggaatt gtcctctttt
    10081 caattagtgg gagtgaatgc ttttcgccct catattgctg tcatcactaa tttaatgccg
    10141 actcacctgg actatcatgg cagttttgag gattatgttg ctgctaaatg gatgattcaa
    10201 gctcagatga cagaatcaga ctaccttatt ttaaatgcta atcaagagat ttcagcaact
    10261 ctagctaaga ccaccaaagc aacagtgatt cctttttcaa ctcaaaaagt ggttgatgga
    10321 gcttatctga aggatggtat actctatttt aaagaacagg cgattatagc tgcaactgac
    10381 ttaggtgtcc caggtaggca caacattgaa aatgccctag caactattgc agttgccaag
    10441 ttatctggta ttgctgatga tattattgcc cagtgccttt cacattttgg aggcgttaaa
    10501 catcgtttgc aacgggttgg tcaaatcaaa gatattacct tctacaatga cagtaagtca
    10561 accaatattt tagccactca aaaagcttta tcaggttttg ataacagtcg cttgattttg
    10621 att
//
LOCUS       DAUCLR010000064        10513 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.064, whole genome shotgun sequence.
ACCESSION   DAUCLR010000064 DAUCLR010000000
VERSION     DAUCLR010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10513
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.064"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            2..469
                     /locus_tag="VGT87_001368"
     CDS             2..469
                     /locus_tag="VGT87_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEQ3814212.1"
                     /translation="MALLQKKEYSKITVRDMITLANVGRSTFYAHYESKEMLLKELCE
                     ELFHHLFRQKRNVTFEDYLVHILKHFEQNKDSIATLLLSNDPYFLLRFKNELEHDVYP
                     NLRCKYIDKTTIPEVFLKQFVLSSFVETLKWWLHQRQRMSANELLKYYLELIK"
     gene            684..1202
                     /gene="rimM"
                     /locus_tag="VGT87_001369"
     CDS             684..1202
                     /gene="rimM"
                     /locus_tag="VGT87_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEQ3814213.1"
                     /translation="MEYFNVGKIVNTQGLQGEMRVLSVSDFAEERFKKGSQLALFDDK
                     DRFVQEVTIVSHRKQKHFDIIKLKDMYHINAIEKYKGYTLKVSKDNQGDLQEGEFYYH
                     QIIGMAVYEKDRLIGYVKEILQPGANDVWVVKRQGKRDLLLPYIPPVVLNVDVPNKRV
                     DVELMEGLDDED"
     gene            1192..1923
                     /gene="trmD"
                     /locus_tag="VGT87_001370"
     CDS             1192..1923
                     /gene="trmD"
                     /locus_tag="VGT87_001370"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEQ3814214.1"
                     /translation="MKIDILTLFPEMFAPLEHSIVGKAKEKGLLDIHYHNFRDYAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDTIEQIEAKKPRIILLDPAGKPFTQAYAEELALEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDFVLTGGELAAMTMVDATVRLIPQVLGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMTVPDVLMSGHHERIRLWRLEESLRKTYLRRPDLL
                     ERYDFSEEERKLLDKIKEALGQGED"
     gene            1923..2915
                     /locus_tag="VGT87_001371"
     CDS             1923..2915
                     /locus_tag="VGT87_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEQ3814215.1"
                     /translation="MKDKAYDITIIGGGPIGLFAAFYAGLRGVTVKIIESLSELGGQP
                     AILYPEKMIYDIPAYPSLTGAELTENLIKQLSRFEDRTTICLKEEVLTFDKVKGGFSI
                     RTNKAEHFSKAIIIACGNGAFAPRTLGLESEENFADHNLFYNVHQLDQFAGQKVVICG
                     GGDSAVDWALALEDIAESVTVVHRRDAFRAHEHSVELLKASTVNLLTPYVPKALKGIG
                     NLAEKLVIQKVKEDEVLELELDSLIVSFGFSTSNKNLKNWNLDYKRSSITVSPLFQTS
                     QEGIFAIGDAAAYNGKVDLIATGFGEAPTAVNQAINYIYPDRDNRVVHSTSLID"
     gene            3093..4151
                     /locus_tag="VGT87_001372"
     CDS             3093..4151
                     /locus_tag="VGT87_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HEQ3814216.1"
                     /translation="MTTTDKETFSSFMNKVLAGTAIAIVVALIPNAILATFLKPLLPN
                     MAAAEFLHIVQVFQFFTPIMAGFLIGQQFKFNPMQQLAVGGAAYIGSGAWAYTEVIQK
                     GVATGTFQLRGIGDLINMMITASLAVLAVKYFGNKFGSLTIILLPITIGTGVGYIGWK
                     FLPYVSYVTTLIGQGINSFTTLQPILMSILIAVAFSLIIVSPISTVAIGLAIGLNGMA
                     AGAASMGIASTAAVLVWATLKVNKSGVPIAIALGAMKMMMPNFLKHPIMAIPMVFTAA
                     ISSLTVPLFNLVGTPASSGFGLVGAVGPIASLAGGSSILIIILAWIIVPFAVAFAAHK
                     VSKDILKLYKEDIFVFEG"
     gene            4164..5087
                     /locus_tag="VGT87_001373"
     CDS             4164..5087
                     /locus_tag="VGT87_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEQ3814217.1"
                     /translation="MLVYIAGSGAMGCRFGYQISKTNNDVILLDNWEDHINAIKENGL
                     IVTGDVEETVKLPIMKPTEATQEADLIILFTKAMQLPQMLQDIKGIIGKETKVLCLLN
                     GLGHEDVIRQYIPEHNILMGVTVWTAGLEGPGRAHLQGVGALNLQSMDPSNQEAGHQV
                     ADLLNEANLNATYDENVVPNIWRKACVNGTMNSTCALLDCTIGELFASEDGLKMVKEI
                     IHEFVIVGQAEGVKLNEEEITQYVMDTSVKAAHHYPSMHQDLVQNHRLTEIDFINGAV
                     NTKGEKLGINTPYCRMITELVHAKEAVLNIQ"
     gene            5343..6056
                     /locus_tag="VGT87_001374"
     CDS             5343..6056
                     /locus_tag="VGT87_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEQ3814218.1"
                     /translation="MAKITEENYVSLEDLMQLLNSSESTIRRDLGELEQEGRLHRVHG
                     GAELFHSLQEELSNQEKSVKNSHIKKAIAQRASQFIYDNDVIFIDAGTTTEFLLPFLQ
                     AKNLTVVTNSIHHAARLVELSIETIIVGGYVKQTTDASIGNVALEQIRQMNFDKAFLG
                     MNGVDDSYLTTPDMEEAVIKKAVLSNAKLAYILVDGTKIGQVSFVKVAPINDVTIITL
                     GGSASILKQIKEKAKVIEL"
     gene            6053..6964
                     /gene="pfkB"
                     /locus_tag="VGT87_001375"
     CDS             6053..6964
                     /gene="pfkB"
                     /locus_tag="VGT87_001375"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEQ3814219.1"
                     /translation="MIYTVTLNPSIDFIVRIDQINLGSVNRMASDDKFAGGKGINVSR
                     ILQRLDITSTATGFLGGFTGRFIEESLSAEGVKTDFVKGDQDTRINVKIKSQEETELN
                     GQGPIISREQLEELKTKLSQLTAEDTVVFAGSAPANLGNAVYKELLPLVRQSGAQVVC
                     DFEGQTLIDALTYNPLLVKPNNHELEAIFGTILTSLDDVETYARRLLEMGAQNVIISM
                     AGDGALLVTKEATYFAKPIKGEVKNSVGAGDSMVAGFTGEFMKSQNPIEALKWGVACG
                     TATAFSDDLATIAFIKETYHKVEVEKR"
     gene            6961..8907
                     /locus_tag="VGT87_001376"
     CDS             6961..8907
                     /locus_tag="VGT87_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEQ3814220.1"
                     /translation="MKIQDLLRKDIMILDLQAISKEVAIDEMITKLVEKDIVHDFDVF
                     KKSIMIREEQTSTGLGDGIAMPHSKNIVVDKPAVLFAKSNKGVDYKALDGQPTDLFFM
                     IAAPQGANDTHLAALAELSQYLLKDGFADKLRAAATPEAVIAVFDEASTAKEEVVAPT
                     SGQDFIVAVTACPTGIAHTYMAEEALKKQAAEMGVAIKVETNGASGVANRLTAEDIQR
                     AKGVIVAADKAVEMDRFDGKQFIARPVADGIKKSQELISLILNNEGNTYHAKNGKSET
                     AVSTEKTSLGGAFYKHLMGGVSQMLPFVIGGGIMIALAFLLDNMLGVPNDQLGSLGSY
                     HEIAAIFMNIGGAAFSFMLPVLAGYIAYSIAEKPGLVAGFVAGAIASNGLAFGKVPFA
                     AGGEVSLGLTGVPSGFLGALVGGFLAGGVILALRKLLAGLPRSLEGVKSILLYPLLGV
                     LVTGFLMLFVNIPMAAINTALNDFLQGLSGSSAVLMGLLVGGMMAVDMGGPVNKAAYV
                     FGTGTLAATVANGGSVVMAAVMAGGMVPPLAVFVATLLFKDKFTKEERESGLTNIVMG
                     LSFITEGAIPFGAADPARAIPSFIAGSALTGALVGLAGIKLMAPHGGIFVIALTSNPI
                     LYLVFVVIGALVSGILFGALRKKA"
     gene            9000..9599
                     /locus_tag="VGT87_001377"
     CDS             9000..9599
                     /locus_tag="VGT87_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEQ3814221.1"
                     /translation="MKVKKRRRRAKSSVNRLVLGLVLLNLIVSMWTLKLGNQRLAPYA
                     DHETLTFVRKISHAAQSVAQKKQLYSSVMMAQAILESNNGKSQLSQKPYYNFFGIKGS
                     YKERSVIFPTLEDDGQGNLYQIDAAFRSYGSLTACFLDYARVLNDSLYDKTHKKFWSH
                     YQDATATLTGTYATDTTYHTKLNELIEWYQLTNFDGLMK"
     gene            9750..10457
                     /locus_tag="VGT87_001378"
     CDS             9750..10457
                     /locus_tag="VGT87_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEQ3814222.1"
                     /translation="MTKKKGKLVLISLFVLAACLGAYSAMRQSHKTSNVSAETIASSS
                     TRHFIDEIGPTASTIGQERDLYASVMIAQAILESSNGKSSLSQAPYYNFFGIKGAYNG
                     SSVTMSTWEDDGNGNTYTIDQAFRAYPSIADSLNDYADLLSSSTYIGARKSNTLSYQD
                     ATAALTGLYATDTSYNLKLNNIIATYGLTAYDVANSAAQETGLATSGYVWNEYRRNYT
                     DAETLAVDEAWAKRMTY"
BASE COUNT         3230 a         1731 c         2283 g         3269 t
ORIGIN      
        1 tattgcttta ttacaaaaaa aagaatattc gaaaataact gttcgagata tgattacact
       61 tgctaatgta gggagatcaa ccttttatgc acattatgaa agcaaagaaa tgttactaaa
      121 agaactctgt gaagaacttt ttcatcattt gtttaggcaa aaaagaaatg tgacttttga
      181 agactatttg gtacatattt taaagcattt tgaacaaaat aaagacagca ttgcgacgtt
      241 gttactttct aatgatccct attttttatt aagatttaaa aacgaactag aacatgatgt
      301 ttaccctaac ttgcgctgca agtatatcga caaaacgact attccagaag tattcctgaa
      361 acaatttgtt ctatctagtt ttgtagaaac cttaaaatgg tggctccatc aaaggcaaag
      421 gatgtctgca aatgaactat taaagtatta tttagagctc ataaaataat gactagtgag
      481 tcatcttgcc aaattcaatt ttttaataca gacgtatttt aacaatttaa cagagaaaga
      541 caaaactgta agtgcaaaat agactataac gtgataaaat aataggtaat ttggttctga
      601 aaaagaagtg tctatcaaac tttagaatca agagaggtcg ctaaagtgac gtgagataaa
      661 gagttaacga tttaggagct accatggaat attttaatgt tggaaaaatt gtcaacacgc
      721 aaggcctgca aggagagatg cgcgtgttgt ctgtcagtga ttttgctgag gaacgcttta
      781 aaaaaggctc acaattagct ttatttgatg ataaagatcg gtttgttcaa gaagtgacaa
      841 ttgttagtca ccgtaaacaa aagcattttg atattatcaa attgaaagac atgtatcata
      901 ttaatgctat tgaaaagtac aagggataca ctttaaaggt ctcaaaagat aatcaagggg
      961 atttacaaga aggagaattt tattaccatc agattattgg catggctgtt tatgaaaagg
     1021 accggttgat tggctatgtc aaggaaatct tgcagccagg tgctaatgat gtttgggtag
     1081 tgaaacgcca aggaaaacga gacttgctct taccatatat tcccccagta gttcttaacg
     1141 tagatgttcc taacaagcgc gtggatgttg aattgatgga aggcttagac gatgaagatt
     1201 gatattttaa ccctttttcc tgaaatgttt gcccctctag agcattccat tgtaggcaag
     1261 gcaaaagaaa aaggtctgtt agacattcac taccataact ttcgagatta tgctgaaaaa
     1321 gctcgtcatg tggatgacga accctatggt gggggccaag gcatgttatt gagagcgcag
     1381 cccatttttg acaccattga acagattgaa gccaaaaaac caaggattat tcttttagat
     1441 ccagcaggta aaccctttac tcaggcttat gctgaagaat tagctcttga ggaagagtta
     1501 atttttattt gtggtcatta tgaaggttat gatgagcgga ttaagacctt ggtgacggat
     1561 gaaatttcat taggcgactt tgtattaacg ggaggtgagt tggcggccat gactatggtg
     1621 gatgcgaccg tcaggttaat tccacaggta cttgggaaag aaagtagcca tcaggacgat
     1681 tctttttcat caggactttt agaataccct caatacacaa gaccttatga ttatcgtggt
     1741 atgacagttc cagatgttct aatgagtggt catcacgaac gtattcgctt gtggcgtcta
     1801 gaagaaagtc tcagaaaaac ctatttgaga aggccagatt tgttagagcg gtacgatttt
     1861 tccgaggaag agcgcaagct attggataag attaaagaag cattgggtca aggagaagat
     1921 tagtgaaaga taaagcatat gatattacga ttattggtgg tggtccaata ggtttatttg
     1981 cagcctttta tgcaggtctt aggggcgtaa cagtaaagat tattgagagt ttgtcagaac
     2041 ttggtggaca gcctgccatt ctttatcctg agaaaatgat ttatgatatt ccagcttatc
     2101 catctttaac aggtgctgag ttgacagaaa atttaataaa acaattaagc cgttttgaag
     2161 atcgtacaac catttgttta aaagaagaag tattaacctt tgacaaggtt aaaggtggtt
     2221 tttcgattag gactaataag gcagagcatt tttcaaaagc cattatcatt gcttgtggaa
     2281 atggcgcttt tgcacctaga actttagggt tagaaagtga ggaaaacttt gctgatcata
     2341 acctttttta taatgttcac cagttggacc agtttgcggg acaaaaagtt gtgatttgtg
     2401 gtggtggaga ttcagcagtt gattgggctt tggctttaga agatattgct gagagtgtga
     2461 cggttgtgca ccgtcgtgat gctttcagag cacatgaaca cagtgtggaa ttattgaaag
     2521 catcaacggt caacctctta acaccttatg tgccaaaagc attgaaagga attggaaacc
     2581 tggctgaaaa attagttatt caaaaagtga aagaagatga agtgttagaa cttgaattgg
     2641 acagtcttat tgttagtttt ggtttttcaa cttctaataa gaatttgaaa aattggaatc
     2701 tcgactataa gcgttcaagt attactgttt caccactttt tcaaacaagt caagaaggta
     2761 tttttgccat tggtgatgca gccgcttata atgggaaagt ggatttgatt gctactggtt
     2821 ttggcgaagc accaacagca gttaatcagg ccattaacta tatttatcca gatcgagata
     2881 accgtgtggt gcattcaacc tctttaattg actaagggag tcggaagttt caagagcagt
     2941 agcaacagct aatattcagt ttgtcaggag aggacaaatg gtttttacct aagtcttcgt
     3001 gacaagtttt atactttgtg ataaaatagt cattgtgaaa aaagtgtgat ggttttcaca
     3061 gattaacaca ttacaatgaa aaggggtcta tcatgacaac aacagataaa gagacattta
     3121 gttcttttat gaataaggtc ttggctggta cagcgattgc tatcgttgtg gcactcattc
     3181 caaatgctat tttagcgact ttcttaaaac cgcttttgcc aaacatggca gctgctgaat
     3241 ttttacacat tgtgcaagtc ttccaattct tcacgccgat tatggctggt ttcttgattg
     3301 ggcaacaatt taagtttaat cctatgcaac agttggctgt tggtggagca gcctatattg
     3361 gttctggagc ctgggcttat acagaagtca ttcaaaaagg tgtggcgaca ggaacattcc
     3421 aacttagagg tattggggat ctcatcaata tgatgattac agctagcctt gcggttttag
     3481 ctgtgaaata ttttgggaat aaatttggct ctttaaccat tatcttgtta cctatcacta
     3541 taggaactgg cgtaggttat attggttgga aatttttgcc ttatgtgtct tacgtgacaa
     3601 ccctgatcgg acaagggatt aattctttta caaccttaca acctatttta atgtctattt
     3661 taattgcagt tgctttctcg cttatcattg ttagtccgat ttcaactgtt gcaatcggtt
     3721 tagctattgg gcttaatgga atggctgcgg gagcagcttc tatgggaatt gcatcgacag
     3781 cagcagtctt agtgtgggcg acgcttaaag ttaataaatc tggtgtacca attgctatcg
     3841 cacttggggc tatgaaaatg atgatgccaa acttcttgaa acatcctatc atggctattc
     3901 cgatggtgtt cactgcagca attagctcat taacagtacc attgtttaat cttgttggaa
     3961 caccagcttc atctggtttt ggcttagttg gtgcagtagg tccgattgct tctttagcag
     4021 gtggtagctc aatacttatt attatccttg cttggatcat tgttccgttt gcggttgctt
     4081 ttgcggcgca taaggtttct aaagatattc taaaacttta taaagaagac attttcgtct
     4141 ttgaaggcta ataggaggaa aatatgttag tttatattgc tggctcagga gccatggggt
     4201 gccgttttgg ttaccaaatt tcaaaaacaa ataacgatgt gattttgtta gataactggg
     4261 aagatcatat caatgccatt aaagaaaacg ggttgattgt aacaggtgat gtggaagaaa
     4321 cagttaagct tcctattatg aaaccgactg aagccactca agaagccgat ttgattatct
     4381 tgtttacaaa agcaatgcag ttgccacaaa tgcttcaaga cattaaaggc attattggaa
     4441 aagaaaccaa agtgctttgc cttttaaatg gtcttggaca cgaagacgtt attcgtcagt
     4501 acattccaga gcacaacatt ttgatggggg tcactgtctg gactgctggt ctagaaggtc
     4561 ctggtcgtgc tcatcttcaa ggtgtgggag cacttaactt acaaagcatg gatccaagca
     4621 atcaagaagc tggacaccaa gtggctgatt tgttaaatga agctaacttg aatgcgactt
     4681 atgatgagaa tgttgtgcca aacatctggc gaaaagcttg cgtgaatggt accatgaact
     4741 caacttgtgc ccttctagat tgtacaattg gtgaattatt tgccagtgaa gacggtctca
     4801 aaatggtaaa agaaatcatc catgagtttg ttatcgttgg tcaagcagaa ggtgtcaaac
     4861 taaacgaaga agaaatcaca caatatgtaa tggatacatc tgttaaagct gctcaccatt
     4921 acccatcaat gcaccaagac cttgtgcaaa atcaccgatt gactgaaatt gattttatca
     4981 atggtgcagt caataccaaa ggtgaaaagc ttggtatcaa tactccatac tgccgtatga
     5041 ttacagagct agttcacgct aaagaagcag tgttgaacat ccaataaacg ctatcaaatc
     5101 gttatactca atattatggc taaagcttta atattgagtt ttttgttatg ctgatagtat
     5161 ttttcctagg cgtcattatt tgtgagccta gattatgagt taataaaaga aataataatt
     5221 ggtagaattt gatagatttt gaaaggttat gcttgacttt tagttataaa atagtataat
     5281 aattgtaaac gatttcagtt aggggtggtt ctatcttaaa atcaaaacga aaacaattaa
     5341 tcatggcaaa gatcactgaa gaaaactatg tttctttaga ggatttaatg caattactca
     5401 actcctctga gtctactatc cgtagagatc taggagagtt ggagcaagaa ggacggctac
     5461 atcgtgttca tggaggtgct gaactttttc actctttaca agaggaactt tctaaccaag
     5521 aaaagtctgt caaaaacagt catattaaaa aagctattgc gcaaagagcc tctcagttta
     5581 tctatgacaa cgatgtgatc tttattgatg ctgggacaac gactgaattt ttattacctt
     5641 ttttacaagc taaaaattta actgtagtta ctaattctat tcatcatgct gctagattag
     5701 ttgaactctc tattgagacg attattgtag ggggttatgt caaacaaacg accgatgcca
     5761 gtattggtaa tgtagctttg gaacagatta ggcagatgaa ttttgacaag gcatttcttg
     5821 gaatgaatgg tgttgatgat tcttacctga cgactcctga tatggaagaa gcagttatta
     5881 aaaaagcggt tctgtcaaat gcaaaattag cttacatttt agtagatggc actaaaattg
     5941 gtcaggtatc gtttgtaaaa gtagctccta ttaatgatgt gactattatt acattaggag
     6001 gatcagcaag tattcttaaa caaataaagg aaaaggcaaa ggtgattgaa ttatgattta
     6061 taccgtgacc ttaaaccctt ctattgactt tattgtcaga attgaccaaa taaatcttgg
     6121 gtcagtgaat cgcatggcga gtgatgataa gtttgctgga ggtaaaggca ttaatgtgag
     6181 tcgtatcttg caacgtttag acattactag cacggcgaca gggttcttgg gtggttttac
     6241 aggacgtttt atcgaagaaa gtttgagtgc agaaggcgtt aaaactgatt ttgtaaaagg
     6301 tgatcaagat actcgaatca atgttaaaat caagtcacaa gaagagaccg aattaaatgg
     6361 tcaaggacct atcattagtc gggaacaact agaggagttg aaaactaagc tttcccagct
     6421 aacagcagag gatacagttg tctttgcagg ttcagcgcca gctaatcttg gcaatgctgt
     6481 ttataaggaa ttacttcctt tagttaggca aagcggtgct caggtggttt gtgattttga
     6541 aggacagact ttaatagatg cgcttacata taatcctctg ttggttaaac ctaataatca
     6601 tgaactagaa gctatttttg gtacgattct gactagtctt gatgatgttg aaacctatgc
     6661 ccgtcgtctt ttagagatgg gtgctcaaaa cgtcatcatt tcaatggctg gtgatggtgc
     6721 tttgctggtt actaaagaag cgacttattt tgctaaaccg attaagggag aagttaaaaa
     6781 ttctgtcggt gctggtgatt cgatggttgc tggctttaca ggcgaattta tgaagagtca
     6841 aaatccaata gaagccttaa aatggggcgt ggcttgtggt acagcgacgg ctttctctga
     6901 tgacttggcg acaattgcat ttattaagga aacatatcat aaagttgagg tagaaaaacg
     6961 atgaaaattc aagatttatt gagaaaagat attatgattc tcgatttaca ggctatttct
     7021 aaagaagttg ccattgacga gatgattact aaattagttg aaaaagatat tgtacatgat
     7081 tttgatgtct ttaaaaagag tatcatgata cgtgaagaac aaacatcaac agggcttggt
     7141 gatgggattg ccatgcctca ttctaaaaat attgtagtag ataaaccagc ggtcttgttt
     7201 gcgaaatcaa ataaaggcgt ggattataag gctttagatg gtcaaccaac ggatctcttc
     7261 tttatgattg ctgcgcctca aggagcaaat gatactcact tggctgcgct agcagaatta
     7321 tcacaatacc ttttgaaaga cggctttgct gataaacttc gtgctgcagc gacaccagaa
     7381 gcagtcattg ctgtttttga tgaagcgtca acagctaaag aagaagtggt tgctccaaca
     7441 agtggtcaag actttatcgt tgctgttaca gcttgtccga caggaattgc tcacacttat
     7501 atggcagaag aggctttgaa aaaacaagct gcagaaatgg gagtagctat taaagttgaa
     7561 acaaacggtg cttctggtgt ggctaatcgt ttaaccgctg aagatattca gagagctaaa
     7621 ggagttattg ttgcagctga taaagcggtt gaaatggacc gttttgatgg taaacaattc
     7681 attgctcgcc ctgttgcaga tggtatcaag aaaagtcagg aattgatttc tttgatttta
     7741 aacaatgaag gaaacactta tcatgctaaa aatggaaaat ctgaaacagc agtatcaact
     7801 gagaaaacaa gtttaggcgg tgctttctat aaacacttga tgggcggtgt ctcacaaatg
     7861 ttgccatttg ttattggtgg cgggattatg attgctctag catttttatt ggataacatg
     7921 cttggtgtgc caaatgatca gcttggaagt cttggatcct atcatgaaat agcagctatt
     7981 tttatgaaca ttggtggagc agccttttcc ttcatgttac cagtactagc aggttatatc
     8041 gcttattcta ttgctgaaaa accgggctta gtagctggtt ttgtggcagg tgccattgcc
     8101 tcaaatggtc ttgcttttgg taaggtacca tttgcagcag gaggagaagt gagcttgggc
     8161 ttaacgggcg tgccatcagg tttccttggg gcgcttgtgg gtggtttcct tgccggtggt
     8221 gttattcttg cccttcgtaa attgttggca ggcctaccac gttctctaga aggggttaaa
     8281 tctatcctcc tttacccatt acttggcgtc cttgtcactg gtttcttgat gctttttgtc
     8341 aatattccaa tggcagctat taacacagcc cttaatgatt tcttacaagg tctttcagga
     8401 agctctgctg tccttatggg acttcttgtt ggtggaatga tggctgttga catgggtggt
     8461 cctgttaata aggcagctta tgtttttggt acaggaactt tagcagctac tgtggcaaat
     8521 ggtggttcgg tcgttatggc cgctgttatg gcaggtggta tggtacctcc tcttgctgtt
     8581 tttgtagcca ctctcttgtt taaagataaa tttaccaaag aagaacgtga atcaggcttg
     8641 acaaatattg ttatgggact ttcattcatc accgaaggag caattccgtt tggtgcagct
     8701 gacccagcac gtgcgattcc tagctttatc gcaggttctg ccttgacagg agctcttgtt
     8761 ggtttggctg gtattaaatt aatggcgcca catggtggta tcttcgtaat cgctttaaca
     8821 agcaatccaa tcttatatct tgtctttgtt gtgattggcg cccttgtatc aggtatctta
     8881 tttggcgctc ttcgcaaaaa agcctaaaag gtaaagaact tttttcttac taaaactaga
     8941 atctgtcaag gttctagttt tttgctatac tagagttaaa ggataagaaa cgaggacctt
     9001 tgaaggtgaa aaaaaggaga agaagggcta aatcatcagt aaatcgactt gtcttagggt
     9061 tagtattact taatctgatt gttagtatgt ggacactaaa attgggaaac cagcgattag
     9121 ctccgtatgc ggatcacgaa actctgacat ttgtcaggaa gattagtcat gctgctcaat
     9181 cagtcgctca aaagaaacag ttatacagtt cggtgatgat ggctcaggcc attttagaat
     9241 ccaataatgg caagtcacaa ctaagtcaaa aaccttatta taattttttc gggattaagg
     9301 ggagctataa agaacggtca gtcatttttc caactttaga agacgatgga caagggaatc
     9361 tttatcaaat tgatgctgct tttcgttcct atgggagcct gacagcttgt tttttagatt
     9421 acgcaagagt tttgaatgat tcactttatg acaaaaccca caaaaagttt tggtctcatt
     9481 atcaagatgc tactgcaacc ttaacaggca cttacgccac tgatacaact taccatacca
     9541 aattaaatga attgattgaa tggtatcaac tcactaattt tgatggtcta atgaaatagt
     9601 tatccacaat tttaaaaaaa tcagaagaaa tatgtcgatt tcaagacaaa agttatcaat
     9661 ctcattaaat tatctgtgga aaagttaatt taaacgttcg tgtgctataa tagaaacata
     9721 gaaaatgtat aaaaaaggat atgtgaataa tgacaaaaaa gaaaggtaag cttgtactta
     9781 tcagtctttt tgttctagca gcttgtttag gagcttatag tgcaatgaga caatctcata
     9841 aaacttcaaa cgtgtcagct gagactatcg ccagttcttc aacccgacat tttattgatg
     9901 aaattggtcc aactgctagt actattggtc aagaacgtga tctttatgcg tctgttatga
     9961 tagcacaagc gattttagaa tcaagtaatg gtaaatcaag tctaagtcaa gcaccttatt
    10021 ataatttttt cggtattaag ggtgcctata acggttcttc tgtgacgatg tcaacttggg
    10081 aagatgatgg taatggcaat acctatacga ttgatcaagc ctttagagct tatccaagta
    10141 ttgcagactc ccttaatgac tatgcagact tgctaagttc tagcacttat ataggtgcta
    10201 gaaaatcaaa cacgctatcg tatcaagatg ctacagcagc tctaactggc ttatacgcta
    10261 cagacaccag ttataactta aaactaaata atattattgc aacctatggt ttgacggctt
    10321 atgatgttgc taatagcgca gctcaagaaa ctggtctagc aacttcgggt tatgtttgga
    10381 atgagtatcg tcgtaattac actgatgcgg agaccctagc agttgatgag gcttgggcta
    10441 aacgcatgac ttattaatgt aacactaaca acaatctgct tgcactgacc ccaaaaaagc
    10501 tggatactct att
//
LOCUS       DAUCLR010000065        10295 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.065, whole genome shotgun sequence.
ACCESSION   DAUCLR010000065 DAUCLR010000000
VERSION     DAUCLR010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10295
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.065"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            49..426
                     /locus_tag="VGT87_001379"
     CDS             49..426
                     /locus_tag="VGT87_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEQ3814223.1"
                     /translation="MYQTKITGNRLYHTVSKGYGDSVTLFGKTDQGETPMSLLVIALS
                     SCVTMCVQGFYHRQFQQDTLAVSVEASYEEEVFELLVRIDDVLDDKKKADLLAYIGTY
                     CRVKQLLKPEVTVTITLEGLSHV"
     gene            419..805
                     /locus_tag="VGT87_001380"
     CDS             419..805
                     /locus_tag="VGT87_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HEQ3814224.1"
                     /translation="MSDASLKTYQKQWAYQKYWVMAHSQQHYNALRELFKGNQWSEEK
                     VLTFYCLIEEAQAIPPTVKSLRTAYQHVWGYFKKVASQEEKDHFKDLDAQLETKSEEM
                     LCFLQEMTAHYQPSYLLSCRLITKGP"
     gene            878..1954
                     /locus_tag="VGT87_001381"
     CDS             878..1954
                     /locus_tag="VGT87_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ3814225.1"
                     /translation="MFLALNEMKQSKLRYGLIAGLLCLVAYLMFFLSGLAFGLMQENR
                     SAIDLWKADSVLLAKDADATLTLSQVSRAQENQITADKVAPLAQLNIVAWSVKNPKDA
                     DKVKVSLFGIDSNSFIRPNIVKGRLFKTNKEVVLDQSLAKEEAFAIGKDFYTSSSSQA
                     LTIVGYTQNARFSVAPVVYMNLEAFETLKYGEPLPKDKQVVNAFITKGSLTDYPKKDF
                     QKLDIKTFITKLPGYSAQLLTFGFMISFLVIISAIIIGIFMYILTIQKAPIFGIMKAQ
                     GISNKTITTAVLMQTFFLSFLGSGLGLLGTWLTSLLLPTVVPFQSNWFLYLAIFVSMI
                     CFALLGTLFSVFNIIRIDPLKAIG"
     gene            1964..2632
                     /locus_tag="VGT87_001382"
     CDS             1964..2632
                     /locus_tag="VGT87_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ3814226.1"
                     /translation="MSVLTFKQVTKTFQDGHHEINALKATDFSIEAGEFVAIIGPSGS
                     GKSTFLTIAGGLQTPSSGQLIIDGTDYTHLSEKERSHLRFKSVGFILQASNLIPFLTV
                     QQQLELVDHLTGSKEKAKANQLFDDLGIARLKHQLPQELSGGERQRAAIARALYHDPA
                     LILADEPTASLDTEKAYEVVKLLAKESKEKNKAIIMVTHDDRMLEYCDKVYRMQDGEL
                     RQER"
     gene            complement(2736..3653)
                     /locus_tag="VGT87_001383"
     CDS             complement(2736..3653)
                     /locus_tag="VGT87_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEQ3814227.1"
                     /translation="MKTDDLRESQQVEDRRGQSSGSFGGGGLGGGLLLQLLFSRGGWK
                     TKLVILLLLLVMGGGGLSGVLGGKPSSTNNNAYQSSQVTRTNGDKASQEQVSFVSKVF
                     ASTEDYWTKTFREKGLTYHKPTLVLYTGATQTACGRGQASSGPFYCPGDQKVYLDISF
                     YNELSTKYGAKGDFAMAYVIAHEVGHHIQNELGIMDNYASARQGKSKAKANQLNVKLE
                     LQADYYAGAWANYVQGQGLLEKGDIEEAMAAAHAVGDDTLQEETYGRAVPDSFTHGTS
                     KQRQRWFDRGYQYGDFEHGDTFSIPYSKL"
     gene            3804..7019
                     /gene="rexB"
                     /locus_tag="VGT87_001384"
     CDS             3804..7019
                     /gene="rexB"
                     /locus_tag="VGT87_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEQ3814228.1"
                     /translation="MKLIYTEMSYSMTEILVNEARKAADQGYRVFYIAPNSLSFEKER
                     EVLTLLPERGTFSIIVTRFVQMSRYFTVESSPSKQHLDDTTLAMIFYRALMQLKPEDL
                     PSYGRLQNNSVFIEQLVELYKELKNAQLSVHDLTGLDHPQKQEDLIKIIELAETIMIQ
                     QDYNQDSPLQSFARAIKLGLLNNQLSKTVVVIDGFSRFSAEEDYLLSLLNNNCQEVII
                     GSYVSQKAYQKSFIKGNIYEASLHFLQDLAQKYHIKPVFATSNQVFKPAFSRLTQLFE
                     ATHDFSQVDWQLQKNDLDHFSLWQCHHQKEEIEHVAKSIRQKLYEGYRYKDILVLLGD
                     MDAYQLQIGPIFDKFEIPYYLGKAEPMAAHPLVQFIESLERSQRYNWRREDILNMLKS
                     GLFGCFDDSDIDRFEEYTQFADIKGFTKFSKPFTINSSRQYPLDFLNEMRQDIVLPLQ
                     ELFKSQKQLGASLVDKLILFLKKIRLAENMQGLAQSQLEVEKNEEVWKRFTDILTSFH
                     HIFGQEKLRLSDCLALIKTGMKSAQYRVVPATLDVVTIKSYDLVQPHSKPFVYAIGLT
                     QSHFPKQIHHSGLLSDQERARINEIRNYRHFDIASAENSKKNHQTALSLFNAATKELV
                     LSVPTVINETFDDLSPYLKELISFGLPLLDKGKNYLSYDNSDIGNYKALLSQIIAINR
                     QDLIEMSDQDKMFWTVVLRYLRKQLRKQQLELPTSDYRLSTKPLSKEVIEVCFPKGIP
                     LKLSATALTVFYNNQYNYFLKYVLNLNKTESIHPDSRIHGQYLHRVFERLMKDHTQEP
                     FDNKLKQAIYHTNQESFFQQVYQDNAEAEYSLAILEDIVRSTAPILQLNQNIKVIDQE
                     KNFHLDMGNEILVHGIIDRIDQLSDGSLGIVDYKSSANQFDIGTFYNGLSPQLATYLA
                     ALKQIAPHDINQLFGAMYLHLQDPKLDLVTFKQIDNTLVESIYKALTYKGIFSEVEKE
                     HLSTGAYQTKNALYSNDELETLLNYNKYLYLKAAKHIKKGHFLINPYTSDGKTVQGDQ
                     LKAITRFEADLDMAQARRLVTLPAKEKKECFLTLMRKESHL"
     gene            6980..>10295
                     /gene="addA"
                     /locus_tag="VGT87_001385"
     CDS             6980..>10295
                     /gene="addA"
                     /locus_tag="VGT87_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020905049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEQ3814229.1"
                     /translation="MLFNINEKGEPLVISFAPFLSPEAIKHLQENERCSDQSQKRTAQ
                     QIEAIYTSGQNVLVSASAGSGKTFVMVERILDKILRGVSIDRLFISTFTVKAATELRE
                     RIENKLYSQIAQTTDFQMKVYLTEQLQSLCQADIGTMDAFAQKVVSRYGYSIGISSQF
                     RIMQDKAEQDVLKQEVFSKLFSEFMNQKEAPVFRALVKNFSGNCKDTSAFRELVYTCY
                     SFSQSTENPKIWLQENFLSAAKTYQRLEDIPDHDIELLLLAMQDTANQLRDVTDMEDY
                     GQLTKAGSRSAKFTKHLTIIEKLSDWVRDFKCLYGKAGLDRLIRDVTGLIPSGNDVTV
                     SKVKYPVFKTLHQKLKQFRHLETILMYQKDCFPLLEQLQDFVLAFSEAYLAVKIQESA
                     FEFSDIAHFAIKILEENTDIRQSYQQHYHEVMVDEYQDNNHMQERLLTLLSNGHNRFM
                     VGDIKQSIYRFRQADPQIFNQKFRDYQKKPEQGKVILLKENFRSQSEVLNVSNAVFSH
                     LMDESVGDVLYDEQHQLIAGSHAQTVPYLDRRAQLLLYNSDKDDGNAPSDSEGISFGE
                     VTIVAKEIIKLHNDKGVPFEDITLLVSSRTRNDIISHTFNQYGIPIVTDGGQQNYLKS
                     VEVMVMLDTLRTINNPRNDYALVALLRSPMFAFDEDDLARIALQKDNELDKDCLYDKI
                     QRAVIGRGAHPELIHDTLLGKLNVFLKTLKSWRRYAKLGSLYDLIWKIFNDRFYFDFV
                     ASQAKAEQAQANLYALALRANQFEKSGYKGLYRFIKMIDKVLETQNDLADVEVAAPKQ
                     AVNLMTIHKSKGLQFPYVFILNCDKRFSMTDIHKSFILNRQHGIGIKYLADIKGLLGE
                     TTLNSVKVSMETLPYQLNKQELRLATLSEQMRLLYVAMTRAEKKVYFIGKASKSKSQD
                     ITDPKKLGKLLSLALREQLLTFQDWLLAIADIFSTEDLYFDVRFIEDSDLTQESVGRL
                     QIPQLLNADDLKDNRQSETIARALDMLEAVSQLNANYEAAIHLPTVRTPSQLKATYEP
                     LLEPIGVDIIEKSSRSLSDFTLPHFSKKAKVEASHIGSALHQLMQVLPLSKPINQQTL
                     LDALRGIDSNEEVKTAL"
BASE COUNT         3323 a         1810 c         1985 g         3177 t
ORIGIN      
        1 cagttcaaat gattggagaa aaacatcaga aggtggagga aaatcagaat gtatcaaaca
       61 aaaataacag gcaaccgcct ttaccacacg gtttcgaaag gctacggaga tagtgtaacc
      121 ttatttggaa aaactgatca aggggaaacc cctatgagtt tactggttat tgccttgtct
      181 tcttgtgtga ctatgtgtgt gcagggtttt taccatcgtc aatttcagca agacacactt
      241 gctgtttctg ttgaggctag ttatgaggaa gaagtttttg aactacttgt taggattgat
      301 gatgttttag atgacaaaaa gaaagctgat ttattggctt acattggaac ttattgtcgt
      361 gtgaaacaac tcctcaaacc tgaagttacg gtgaccatta cgttggaagg actgtctcat
      421 gtctgatgct agtttgaaaa cttaccaaaa acaatgggct tatcaaaagt attgggtcat
      481 ggcacactca cagcagcatt acaatgcttt aagagaattg tttaaaggaa atcagtggtc
      541 agaagaaaag gtgttgactt tttattgttt gattgaagaa gcgcaagcca ttcctcccac
      601 agtaaaaagc ttacgaacgg catatcagca tgtttggggg tattttaaaa aggtagcgag
      661 tcaggaagaa aaagaccatt tcaaagattt ggatgctcag ttggaaacta aatctgagga
      721 gatgctatgc tttttacaag agatgactgc tcattatcag ccctcttatc tgctatcttg
      781 ccgtttgatt accaaaggtc cttaaatgct aaacagaaaa gtagggattt ctgggaagtt
      841 atgctaagct agtgatataa ataaggggga atgagagatg tttttagcgc ttaatgaaat
      901 gaaacaatcc aaattgcggt atggtttgat tgctggtttg ctatgtttgg ttgcctattt
      961 gatgttcttt ttgtcaggat tggcctttgg tttgatgcag gagaaccgct cggcgattga
     1021 tctttggaaa gctgacagtg ttttactagc caaggatgct gacgctacct tgaccttatc
     1081 acaggtgtcc agagctcaag aaaatcaaat aacagcagac aaggtagctc ctcttgctca
     1141 actcaatatc gtggcgtggt cagttaaaaa tcctaaggat gccgacaaag ttaaggttag
     1201 ccttttcggg attgattcta atagctttat tcgtcctaac attgtaaaag gtcgattatt
     1261 taagactaac aaagaggttg ttttggatca aagccttgca aaagaggaag cttttgcgat
     1321 tggcaaggac ttttacacat cgagttctag tcaagcatta actatcgttg gttatactca
     1381 aaatgctaga tttagtgttg caccagtggt ttatatgaat ttggaagctt ttgaaacatt
     1441 aaaatatgga gaaccactac caaaagataa gcaagttgtt aatgctttta tcactaaagg
     1501 aagtttaaca gattatccta aaaaagactt ccaaaaatta gatattaaaa cctttattac
     1561 taaattacct ggttatagcg ctcaactttt aacttttggc tttatgatta gttttcttgt
     1621 cattatttca gctattatta ttggtatttt tatgtatatt ttgactattc aaaaggcacc
     1681 tatttttggg attatgaaag cgcaaggaat ttctaacaaa acgattacga ccgctgtgct
     1741 tatgcagaca ttctttttga gttttttagg tagtggttta gggttgctag gtacttggct
     1801 gacatcatta ttattaccaa cagtagtacc ttttcaaagc aattggtttt tgtatttggc
     1861 tatattcgtt agtatgatct gttttgctct attaggcacc ttattttctg tttttaacat
     1921 tatacgaatt gatcctttga aagcaattgg ataggagata gtgatgtcgg ttttaacatt
     1981 taaacaagtg accaaaacat ttcaagatgg tcatcacgaa atcaacgcat taaaggcaac
     2041 tgacttttcc attgaagctg gagaatttgt ggctattatt ggaccatcag gttctggaaa
     2101 atcaaccttt ttgaccattg ctggtggctt acagacccca agctctggtc agttaatcat
     2161 tgatggcact gattatacgc atttatcaga aaaagagcgc tcacacttgc gcttcaaatc
     2221 cgttggtttt atcttacaag cttccaatct gatacctttt ttaacggtcc agcagcagtt
     2281 ggaattggtc gatcatttga caggaagcaa agaaaaagcc aaagccaatc agttatttga
     2341 tgatttaggc attgcaaggc taaaacatca gttacctcaa gaattatccg gcggagaaag
     2401 gcaaagagcg gctattgccc gtgctcttta ccatgatcca gcactaatat tggcagatga
     2461 acctactgca agtttggata ctgaaaaagc ctatgaagtg gtcaaactgt tggctaagga
     2521 aagtaaagaa aaaaacaagg ccattattat ggtcactcat gatgatcgga tgctagaata
     2581 ttgtgacaaa gtctatcgga tgcaagatgg agagttacgt caagagagat agtagactgg
     2641 taagagttta tgatattaga agaaaaaacc aaacaagcac agcacttgtc tggttttttt
     2701 ctaagtgtga ctattatcaa tacgtcacca tttccctaaa gcttactata cgggatgcta
     2761 aaggtatcac catgctcaaa gtcgccatat tggtaacctc ggtcaaacca acgttggcgt
     2821 tgcttagaag ttccgtgggt aaaactatca ggaacggctc tgccataggt ttcttcttgc
     2881 agagtatcat ccccaactgc gtgagctgct gccattgcct cctcaatgtc tcctttttcc
     2941 aaaaggcctt gcccttgaac ataattagcc caggcaccag catagtaatc agcttgtaat
     3001 tccaacttga cgttgagctg attggcttta gcctttgatt ttccttgtct agcgctagcg
     3061 taattatcca taattcccaa ttcattttgg atgtggtggc caacttcatg agcaatcaca
     3121 taggccatag caaaatcacc ttttgcgccg tacttggttg atagttcatt ataaaaagaa
     3181 atatctaaat acactttttg atcgcctggg caatagaaag gaccagaaga ggcttgcccc
     3241 ctgccacaag cggtttgagt tgctccagta tatagaacta atgttggttt atgataagtt
     3301 aaccctttct cacgaaatgt cttagtccaa tagtcctcgg tagaggcgaa gaccttactg
     3361 acaaaagaaa cctgttcttg actagccttg tcaccatttg tacgcgttac ttggcttgat
     3421 tgataggcat tgttgttggt agaagaaggc ttgcctccta aaacaccaga taggccacct
     3481 cctcccataa ctaggagaag gagaagaata accagcttgg ttttccaacc tcctcgtgaa
     3541 aagagtaatt gaagcaggag accgccccct aggccgccgc ccccaaaaga accagacgat
     3601 tgaccacgtc tatcttcaac ctgttgactt tctctcaaat catctgtttt cataagtttc
     3661 tcccatctat ttcttgattt tattataaaa taattctatt agaattgcta tttttatgat
     3721 gagattataa ctaaaaatag gaaaagcaat aggtatttgc tataatataa caagggtaga
     3781 ttcagtttgt ataaaggagt catatgaaat taatttacac agagatgagt tactcgatga
     3841 cagagatttt ggtgaatgaa gcacgaaaag ctgctgacca aggttatcga gtgttctata
     3901 ttgctccaaa ttctctttca tttgaaaagg aaagagaagt cttaacctta ttgcctgaac
     3961 gagggacttt ttcgataata gtcacccggt tcgttcaaat gtcaaggtat tttacagttg
     4021 agagctctcc ttctaagcaa catctagatg acacgacttt ggctatgatt ttttatcggg
     4081 ccttgatgca attaaaacca gaagatttgc cgagttatgg tcgtttgcaa aataattcgg
     4141 tttttattga acaactagtt gagttatata aggaattaaa aaacgcacaa ttaagcgtac
     4201 atgatttaac aggattagac catcctcaaa aacaagaaga tcttattaaa attattgagc
     4261 ttgcagagac gatcatgatt cagcaagatt ataatcaaga tagtcctcta caatcttttg
     4321 caagagcaat taagttagga cttttaaata accaactatc aaaaacagtt gttgttattg
     4381 atggtttttc acgtttttca gctgaagagg attatttatt atcactttta aataacaact
     4441 gtcaagaggt catcattggc agttatgtga gtcaaaaagc ttatcaaaaa tcttttatta
     4501 agggaaatat ctatgaggct agccttcatt ttttgcaaga tttggctcaa aaataccata
     4561 tcaagccggt atttgcgacc tctaatcaag tgtttaaacc agccttttca cggctaacac
     4621 aattatttga agcaactcat gatttttcac aagtagactg gcaactccaa aagaatgatc
     4681 ttgatcactt tagcctttgg caatgtcatc atcaaaaaga ggaaattgaa catgtagcaa
     4741 aatccattcg gcaaaagcta tatgaaggtt accgttataa ggatatctta gtgttgctag
     4801 gagatatgga tgcttatcaa ttacaaattg ggcctatctt tgataagttt gaaatcccct
     4861 actatttagg aaaagcagag cctatggctg cccatccatt agtccaattt atagaatcgc
     4921 tcgaacgaag tcagcgctat aattggcgtc gtgaagacat tttaaacatg ttaaaatcag
     4981 gactttttgg ttgttttgat gatagtgata ttgatcgctt tgaggaatat actcagtttg
     5041 cagatattaa agggtttact aaattttcaa agccttttac cattaactct tctaggcagt
     5101 atcctcttga ctttttaaat gaaatgagac aagacatcgt tttacctttg caagaattgt
     5161 ttaagagtca aaaacaactt ggtgcttcac ttgttgacaa attaatactt tttttaaaaa
     5221 agattagatt agctgagaat atgcaaggtt tagcacagtc ccaattagag gtggaaaaaa
     5281 acgaagaagt ttggaaaagg tttacagata ttttgacttc ttttcatcat atctttggcc
     5341 aagaaaaatt acggctatca gattgccttg ctttaatcaa aacgggaatg aaatcagctc
     5401 agtatcgtgt tgttccagcg acattagatg ttgtcactat taaatcttat gaccttgttc
     5461 aaccacattc aaaacctttc gtttatgcaa ttggtctaac tcaatcacat tttcctaagc
     5521 aaattcacca ttcaggacta ctttctgatc aagagcgtgc tagaattaat gaaataagaa
     5581 attatcgtca ttttgatatt gctagtgccg aaaatagtaa aaaaaaccat caaactgcgt
     5641 tatctctgtt taatgctgct actaaagagt tagttcttag tgttccaacg gtaatcaacg
     5701 agacattcga tgatctatct ccttatctta aagaattaat aagctttggg ctacctcttt
     5761 tggataaagg caaaaactat ctatcttatg ataatagcga tattggtaac tataaagctc
     5821 tcttgtcaca aattattgct attaaccgac aagatcttat agaaatgtca gatcaagata
     5881 agatgttttg gactgttgtc ttacgttatc tcaggaaaca gttaagaaag caacagctag
     5941 agctacctac ttcagattat cgtttatcaa caaagccttt gtctaaagaa gttatcgagg
     6001 tatgttttcc aaagggaatc cctttaaagc tatctgcaac tgctttaacc gttttttata
     6061 ataatcagta caactatttt ttgaaatatg ttttgaattt aaacaaaaca gaatccattc
     6121 atccagattc cagaattcat ggtcaatact tgcatcgtgt ctttgagcgg ttaatgaaag
     6181 atcacacgca agaaccgttt gataataaat taaaacaagc aatctaccat accaatcaag
     6241 aaagtttttt tcagcaggtt taccaagata atgccgaggc tgagtacagt ttagctatac
     6301 tagaagatat tgttagatca acagcaccta tcttgcaact taaccaaaac ataaaggtca
     6361 ttgatcaaga aaagaacttt catctggata tgggaaatga gatattagtc cacgggatca
     6421 ttgatcgtat tgaccagctg agcgacggta gcttgggcat tgttgactat aaatcaagcg
     6481 cgaatcaatt tgatattggg accttttata atgggttgag tccacaacta gcgacttacc
     6541 tcgcagcctt aaaacagatc gcaccgcacg atataaacca attatttggg gctatgtacc
     6601 ttcatttgca agatcctaaa ttggatctag taacatttaa acaaattgat aacacattag
     6661 tggagagtat ctataaagcg ttgacctata aaggtatttt ttcagaagtc gaaaaagagc
     6721 atctatctac tggtgcttac caaaccaaaa atgctcttta ttccaatgac gaattggaaa
     6781 cacttttaaa ttataacaag tatctctatc ttaaagctgc taaacacatt aaaaagggac
     6841 attttttgat taatccttat acaagcgatg gtaagacagt tcaaggagat caattaaaag
     6901 ccattacccg ttttgaagca gatcttgaca tggcacaggc aaggcgacta gtgactctcc
     6961 ctgctaagga gaaaaaagag tgctttttaa cattaatgag aaaggagagc cacttgtgat
     7021 ttcttttgcc ccatttttaa gccccgaagc tattaaacat ttacaagaaa acgaaaggtg
     7081 cagtgatcag tctcaaaaac gcacagctca acaaattgaa gcaatttata ctagtggcca
     7141 aaatgtactt gtatcagctt ctgctggttc aggaaaaacc tttgtaatgg tcgaacgcat
     7201 acttgataaa attttgagag gtgtttcaat tgatcggctt tttatctcaa cctttactgt
     7261 taaagcagct acagaactgc gtgaacggat tgaaaacaaa ttatactcac aaattgctca
     7321 aactacagat tttcaaatga aagtttattt aacagaacaa ttgcaatctc tttgtcaagc
     7381 tgatattggt actatggatg cttttgcaca gaaagtagta agtcgctatg gttatagcat
     7441 tggcatttca tcccaatttc gtatcatgca ggataaagca gaacaagatg ttttaaagca
     7501 agaggtgttt agcaaactct ttagtgagtt tatgaatcaa aaagaggcac cggtgtttag
     7561 ggctcttgtg aaaaattttt ctggtaactg taaagacact tcagctttta gagagttagt
     7621 ttatacttgt tattctttta gccaatcgac agaaaatcca aaaatatggt tgcaagaaaa
     7681 ttttctaagc gctgctaaaa cttaccaaag acttgaagat atcccggatc atgatattga
     7741 actcttactt ttggcaatgc aagacactgc aaatcagcta agagatgtga ctgatatgga
     7801 agattatggg cagctgacta aggcaggtag ccgatctgct aaattcacta aacacttaac
     7861 gatcatagaa aagttgtctg attgggtgcg tgattttaaa tgtttgtatg gaaaagccgg
     7921 attggatcgg ttgatcagag atgtgacagg ccttatacca tctgggaatg atgttacagt
     7981 ctcgaaggta aaataccctg tttttaagac cttgcatcaa aaattaaaac aatttaggca
     8041 tttagaaaca attttaatgt atcagaaaga ctgttttccc ttattggaac agttacaaga
     8101 ttttgtgctt gcgttttcag aagcttattt agctgtcaaa atacaagaaa gtgcttttga
     8161 attttcagat attgcacact ttgcaatcaa aattttagag gaaaatacgg atattcgcca
     8221 atcctatcag caacactatc atgaggtgat ggttgatgaa tatcaagata acaatcatat
     8281 gcaagagcga ctcctgacct tactatcgaa cggtcataat cgctttatgg taggagatat
     8341 caaacaatcg atctatcgat ttcggcaagc cgatcctcag atttttaatc aaaagtttag
     8401 agactatcaa aaaaaacctg agcaggggaa agtgatttta ctcaaagaaa actttcgtag
     8461 ccaatcagag gtgttaaatg tcagcaacgc tgtttttagt cacttaatgg acgaatcagt
     8521 aggagacgtc ttatatgatg agcaacatca gttaatagca ggtagtcatg ctcaaacagt
     8581 cccctatcta gaccgtcgtg ctcagttatt gctatataat agcgataaag atgatggcaa
     8641 cgccccttca gatagtgagg gtatttcatt tggcgaggtt acaattgttg ccaaagaaat
     8701 tattaagctt cacaatgata agggtgtccc ttttgaagac attacgttac tcgtttcttc
     8761 aagaacaaga aatgatatca tttctcatac attcaatcaa tatggtattc ctatagtaac
     8821 agatggtggg cagcaaaact atcttaaatc tgttgaagtg atggttatgt tagatacatt
     8881 acgcaccatt aataacccaa gaaatgatta tgcccttgtg gctttactgc gctcaccgat
     8941 gtttgccttt gatgaagatg atttagcaag aatagcactt caaaaagaca atgagctaga
     9001 taaagattgc ctatatgaca agatacaaag ggctgtgatt ggaagaggtg ctcatcctga
     9061 attgattcac gataccttgc ttggcaagtt aaatgttttt ttaaagacgt tgaaaagctg
     9121 gcgtcgatac gctaagctag ggtcgttgta tgacttgatt tggaaaattt tcaatgatcg
     9181 tttttatttt gattttgtag ctagtcaagc aaaagcagaa caagcacaag ctaatctata
     9241 cgcattagct ctacgtgcta atcagtttga aaaatcgggc tataaaggac tataccgttt
     9301 tattaaaatg attgataagg tacttgagac gcaaaatgac ttagcagatg tggaagtggc
     9361 tgctcctaaa caagctgtta atttaatgac cattcacaag tctaaaggtt tacaatttcc
     9421 gtatgtattt atccttaatt gtgacaagcg cttctcaatg acagatattc ataaatcatt
     9481 tattctgaat cggcagcacg gtatcggtat caagtacctt gcagatatca aaggtttact
     9541 tggtgaaaca acactcaatt ctgttaaagt aagcatggaa accttacctt atcaattgaa
     9601 caaacaagag ttacgcttag caactttatc agaacaaatg cgcttactgt atgttgctat
     9661 gacacgagct gaaaaaaaag tttattttat tggtaaagct agtaagagca aaagtcaaga
     9721 tatcacagat cctaaaaagt taggcaaact tttgtcgctg gctttacgag aacagttatt
     9781 gacattccaa gattggctat tagcaatagc agatatattt tcaactgaag atctttattt
     9841 tgatgttcgc tttattgaag atagtgattt gacacaagag tcagtcggac gacttcagat
     9901 accacagtta ttaaatgcag atgatcttaa agataatcgt caatcagaaa caattgcacg
     9961 ggctttagat atgttagaag cagtgtctca attgaatgcc aattatgaag cagctattca
    10021 tttgccaaca gttcgaacgc ctagccaact taaggcaact tacgagcctt tattagaacc
    10081 cattggtgta gatattatag agaaatcttc tcgatcgcta tctgatttta ctttgccaca
    10141 tttttcaaaa aaagcaaaag ttgaagcaag tcatattgga tcagctcttc atcagttgat
    10201 gcaggtgctc cctttgtcaa aaccgataaa tcaacaaacg cttttagacg ctttaagagg
    10261 aattgatagt aacgaagagg taaaaacagc tcttg
//
LOCUS       DAUCLR010000066        10217 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.066, whole genome shotgun sequence.
ACCESSION   DAUCLR010000066 DAUCLR010000000
VERSION     DAUCLR010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10217
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.066"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(43..984)
                     /locus_tag="VGT87_001386"
     CDS             complement(43..984)
                     /locus_tag="VGT87_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEQ3814230.1"
                     /translation="MITTFETILDKIKAHQTIIIHRHQNPDPDALGSQAGLKEIIAQN
                     FPDKKVLMTGFDEPSLAWISQMDQVTDKDYKEALVIITDTANRPRIDDERYTLGQCLI
                     KIDHHPNDDVYGDFYYVDTSASSASEIIADFAFSQNLTLSDKAAKLLYTGIVGDTGRF
                     LYASTTSKTLSIASQLRHFEFDFAAISRQMDSFPLKIAKLQSYVFEHLTIDESGAAYV
                     LVSQETLKHFDVTLAESSAIVCAPGKIDNVQAWAIFVELTDGNYRVRMRSKEKIINGI
                     AKRHGGGGHPLASGANSANLEENQAIFRELIAVCQEI"
     gene            1378..1827
                     /locus_tag="VGT87_001387"
     CDS             1378..1827
                     /locus_tag="VGT87_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEQ3814231.1"
                     /translation="MALAKIVYASMTGNTEEIADIVANKLQELGHDVDIDECTTVDAS
                     EFENADIAVVATYTYGDGDLPDEIVDFYEDLQDLDLEGKIYGVVGSGDTFYDYFCKSV
                     DDFSEQFALTGAIKGAEPVKVDLAAEDEDIDRLEAFAEQLSQAVNNL"
     gene            2003..2287
                     /locus_tag="VGT87_001388"
     CDS             2003..2287
                     /locus_tag="VGT87_001388"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEQ3814232.1"
                     /translation="MRLEEIRQEINSIDHHLVALLEKRMALVEQVTAYKLANHLPVLD
                     QARENQILDRVSYLVKDQAFEPAIHETFKTIMSLSRQYQTQRLTGGDTND"
     gene            2280..3542
                     /locus_tag="VGT87_001389"
     CDS             2280..3542
                     /locus_tag="VGT87_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEQ3814233.1"
                     /translation="MIKSIATLPKPSLKEALGLIGLALPIGVAVGVVDVIFGKGLLFL
                     SEYRDHHLFLLLPFLALAGLVIVFLYDKLGKEVRQGMGLVFQVGHGQKDQIPPILIPL
                     ILFSTWVTHLFGASAGREGVAVQIGATISHYCQRFVTSQEAARHLLIMGMAAGFAGLF
                     QTPIAAVVFALEVLLVGTLRYCALLPSLVAACVASWTSHSLGLEKFTIVLEETLTITP
                     LTLVKLIGLGLIFGLVGNSFACLLGWFKPYLSQKLPNPYLRIAFIGVLLSICLMIGHV
                     GRYSGLGTHLLAAAFSGQTILTYDWLLKMIVTVISLSAGFQGGEVTPLFAIGASLGIV
                     LAPYLGLPVLLVAALGYTTVFGSATNTFWAPIFIGIEVFGPENALAYFVTSAAAYMVS
                     HRHSIYSYQKVVMINLKDMTVNQMDLKK"
     gene            3657..4004
                     /gene="rplS"
                     /locus_tag="VGT87_001390"
     CDS             3657..4004
                     /gene="rplS"
                     /locus_tag="VGT87_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEQ3814234.1"
                     /translation="MNPLIQSLTEGQLRSDIPNFRPGDTVRVHAKVVEGTRERIQIFE
                     GVVISRKGQGISEMYTVRKISGGIGVERTFPIHTPRVDKIEVIRHGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            4251..4322
                     /locus_tag="VGT87_001391"
     tRNA            4251..4322
                     /locus_tag="VGT87_001391"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4284..4286,aa:Arg,seq:cct)
     gene            complement(4285..4599)
                     /locus_tag="VGT87_001392"
                     /pseudo
     CDS             complement(4285..4599)
                     /locus_tag="VGT87_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004234471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            5007..5576
                     /locus_tag="VGT87_001393"
     CDS             5007..5576
                     /locus_tag="VGT87_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEQ3814235.1"
                     /translation="MKGMNYQDYIWDLGGTLLDNYELSTQAFVQTLAFFSLPGDHDAV
                     YQKLKESTAIAVATFAPNEPEFLHVYRLREADKLAQPIWCLGAKEILEKIATSGSRNF
                     LISHRDCQVNQLLEQAGLLIYFTEVVTASNGFARKPNPESLFYLKEKYDINSGLVIGD
                     RLIDKQAGQAAGFNTLLVDGRKNLLEIVT"
     gene            5577..7529
                     /gene="gyrB"
                     /locus_tag="VGT87_001394"
     CDS             5577..7529
                     /gene="gyrB"
                     /locus_tag="VGT87_001394"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEQ3814236.1"
                     /translation="MIEENKHFEKKMQEYDASQIQVLEGLEAVRMRPGMYIGSTAKEG
                     LHHLVWEIVDNSIDEALAGFASHIKVFIEADNSITVVDDGRGIPVDIQAKTGRPAVET
                     VFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVRVYKNGQIHYQKFKRGAVV
                     ADLEIIGTTDVTGTTVHFTPDPEIFTETTQFDYSVLAKRIQELAFLNRGLKISITDKR
                     SGMEQEEHFHYEGGIGSYVEFLNDKKDVIFETPIYTDGELEGIAVEVAMQYTTSYQET
                     VMSFANNIHTHEGGTHEQGFRAALTRVINDYAKKNKILKENEDNLTGEDVREGLTAVI
                     SVKHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFQRFLLENPQVARKIVEKGILASKA
                     RIAAKRAREVTRKKSGLEISNLPGKLADCSSNDANQNELFIVEGDSAGGSAKSGRNRE
                     FQAILPIRGKILNVEKATMDKILANEEIRSLFTAMGTGFGADFDVSKARYQKLVIMTD
                     ADVDGAHIRTLLLTLIYRFMRPVLEAGYVYIAQPPIYGVKVGSEIKEYIQPGIDQEDQ
                     LKTALEKYSIGRSKPTVQRYKGLGEMDDHQLWETTMDPENRLMARVTVDDAAEADKVF
                     DMLMGDRVEPRRDFIEENAVYSTLDI"
     gene            7897..9621
                     /gene="ezrA"
                     /locus_tag="VGT87_001395"
     CDS             7897..9621
                     /gene="ezrA"
                     /locus_tag="VGT87_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEQ3814237.1"
                     /translation="MSSGIILLIVAIVLLVIIAYLVGVIIRKRNDSLITSLEERKQAL
                     FALPVNDEIEEVKSLHLIGQSQTSFREWNQKWVDLTVNSFADIENHIFEAENLNDTFN
                     FIRAKHEINSVESQLNLVEEDIASIREALNILKEQEEKNSARVTHALDLYEKLQASIS
                     ENEDNFGSTMPEIDKQMKNIETEFSQFVALNSSGDPVEASEVLDRAEEHTIALGQITE
                     QIPAIVAKLEDDFPDQLDDLETGYRRLLEENYHFPEKNIEARFQEIRESIRANSSELV
                     TLDLDRAREENTHIQERIDSLYEVFEREIAAYKVAAKNSKMLPRYLAHVKRNNEQLKD
                     EIARLSRKYILSETESLTVKAFEKDIKEIEDSTLAVAEQFGLQEKPFSELQVTFERSI
                     KTLTNVESGQMDVFAAVKDIEKIESQARHNLDVYVTQLHMIKRYMEKRHLPGIPQDFL
                     SAFFTTSSQLEALMDELSRGRINIEAVSRLLEVATVAIANLEDLTYQVVQNATLTEQL
                     LQYSNRYRSFEAGVQSSFEHALRLFEVENDYQASFDEISYALETVEPGVTDRFVNSYE
                     KTREHIRF"
     gene            complement(9753..10211)
                     /locus_tag="VGT87_001396"
     CDS             complement(9753..10211)
                     /locus_tag="VGT87_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEQ3814238.1"
                     /translation="MTNLEDKLLKGAWGERRLNPEQQRYYLGTYAERVVLSALLDEAQ
                     TKTVKDYLKRELPSLQLVYQPLHLKISSKLAPQLQILYMKLAKENHLPVTIITETHMT
                     SPFAFILHTDHAINLKETRLEVILKQTKNDQLSKKTPEKTKSFWKRFLKK"
BASE COUNT         3096 a         1740 c         2137 g         3244 t
ORIGIN      
        1 aatgacttga caaggctttt ctttccttat tttttaaaaa tcctaaatct cttggcaaac
       61 agcgatgagt tctcggaaaa tagcttgatt ttcttctaaa ttagctgagt tggctccgct
      121 agcaagggga tgcccccctc caccgtgacg cttagcaatg ccattaataa tcttttcttt
      181 actgcgcata cgcacacggt agttgccgtc agttaactca acaaaaatag cccaagcttg
      241 aacgttatca attttaccag gagcacagac aatggcagag ctttctgcta gggtcacgtc
      301 aaaatgtttt aaggtttctt ggctgacaag gacataagca gccccactct catcaattgt
      361 taaatgctca aagacgtagc tttgcagctt tgctattttc aaaggaaacg aatccatttg
      421 ccttgaaatc gcagcaaagt cgaactcgaa atgtctgagt tggctagcaa tggaaagggt
      481 tttactagtg gttgaggcat aaagaaatcg ccctgtatca ccaacgatac cagtgtataa
      541 gagcttagca gccttgtcag agagagtaag attctgacta aaggcaaagt ctgcaatgat
      601 ttcacttgcg ctagaagcgc ttgtgtccac ataatagaag tcaccataca catcatcgtt
      661 gggatggtga tcaatcttaa ttaagcactg ccccagtgtg tagcgctcat catcaatcct
      721 tggtcgattc gctgtatctg taatgatgac caaagcctct ttatagtctt tgtcagtcac
      781 ctgatccatt tggctaatcc aagctaaact aggctcatca aaaccagtca ttaaaacctt
      841 tttgtctggg aaattttgtg caataatttc tttcaagccg gcctgactac caagagcatc
      901 agggtcagga ttttgatggc gatggataat aatagtttgg tgggctttta ttttatctaa
      961 aattgtttca aaagttgtta tcatgcttta atctttcttt ttcaaaaaga ggaaaataac
     1021 cgtttaacat agtcttcaaa aaacggtttg tgtcataagg aggtgtgcgt tatgttaagt
     1081 ggctaatgct agaatttttt aagattgtca aagaccataa attgacattt tatctgaaaa
     1141 tcatatccta ttttattata gcataaaagt aagctctaga taatggtaaa ttaatctagg
     1201 aagaaaagag gttagttatc agttatgata ctatcaaagc tagtcttgca aatgcaagtg
     1261 aaaaaatagt ttttaagtgt tttaagtaat catatcaacc ttctcgttaa acaattagtg
     1321 cttttcctaa aaaagtgtgt taaaataaac atagtttagt aattttggga ggaatttatg
     1381 gcattagcaa aaatcgttta tgccagcatg acgggaaata ctgaagaaat cgctgatatc
     1441 gttgcaaata aattgcaaga attgggccat gatgttgaca ttgatgagtg cacaacggtt
     1501 gacgcttcag agtttgagaa tgctgatatc gctgtcgttg cgacttatac ttatggtgac
     1561 ggtgacttac cagatgaaat cgttgatttt tacgaagatt tacaagattt agatttagaa
     1621 ggtaaaatct atggcgttgt cggctctggt gacacttttt atgactattt ttgcaaatct
     1681 gttgatgact tttcagagca atttgcgctt actggtgcca tcaaaggtgc agagccagtc
     1741 aaagtagatt tggcagcaga agatgaagac atcgatcgtt tagaggcctt tgcagagcaa
     1801 ctctcacagg ctgttaataa tctataaaac tatttagtaa aaatgtcagg gacttccttg
     1861 gcatttttag atttttaacc taaaataaag ggaatgtcac ttcagggtag gaattgaaaa
     1921 aaacaatcag cttcaccttg cttctgatcc tcttattaac tataataaaa agataatcta
     1981 atacatctag aaaaggaaag ttatgcgttt agaagagata cgacaagaaa tcaatagtat
     2041 tgatcaccac ttggttgcct tattagaaaa gcggatggca cttgtagagc aagtaacagc
     2101 ttataaacta gccaatcact tgcctgtctt agatcaggcg agggaaaatc agatcctaga
     2161 tagggtttct tatctcgtta aagaccaagc atttgagcca gctatccatg agacgtttaa
     2221 aaccattatg tctctttcac ggcagtatca aacgcaacgc ttaacaggtg gtgacactaa
     2281 tgattaaatc aattgcaaca ctgcctaaac cctccttaaa agaagcgtta ggactaatag
     2341 gcttggcgct tcccataggg gtcgcggttg gagtggttga tgtgattttt ggtaaaggac
     2401 ttttgtttct ttccgagtat cgcgatcacc accttttctt gcttcttcct tttcttgccc
     2461 tagcaggtct tgttatcgtt tttctttacg ataagttagg caaagaggtt cgtcaaggga
     2521 tgggattggt ttttcaggta gggcatggtc aaaaagatca gattcctcct attctcattc
     2581 ctcttatttt gttctcgact tgggtaaccc acttatttgg agcgagtgcg ggtagagaag
     2641 gggtagccgt tcaaatagga gctaccatct ctcattattg tcagagattt gtgactagtc
     2701 aagaagccgc acgacacctt ttgattatgg ggatggcagc cggttttgca ggactttttc
     2761 aaacgccgat tgctgctgtc gtatttgccc tcgaggtttt attggtagga acgcttcgct
     2821 actgcgcttt attaccttcc ttagttgctg cttgcgtggc ttcttggacc tctcattctt
     2881 tgggattaga gaaatttacg attgttcttg aagaaacatt gaccataact cccttaacct
     2941 tagttaaatt aattgggtta ggtcttattt ttggtctggt gggaaatagt tttgcctgtt
     3001 tacttggctg gtttaaacct tacctgagtc aaaagctacc aaatccttat ctcaggattg
     3061 ctttcattgg tgttttgtta tctatctgtt tgatgattgg tcacgttgga agatatagtg
     3121 gtctagggac tcatttgctt gcggcagctt tttctggcca aactatccta acttacgact
     3181 ggcttttaaa aatgatcgtg acggtgatta gcttatctgc aggctttcaa ggtggtgaag
     3241 tgacgcctct atttgctatt ggagcttctt taggtatcgt tttggcccct tacttaggtc
     3301 ttcctgtact gttggttgct gcacttggtt acaccacggt atttggaagt gcaacaaata
     3361 ccttttgggc ccctatcttt attgggatag aggtctttgg tccagagaat gccctagcat
     3421 attttgtcac ctcggctgca gcctatatgg ttagtcacag acattccatt tacagttatc
     3481 aaaaagtagt catgatcaat ttaaaagaca tgacggtaaa tcagatggat ttaaaaaaat
     3541 agctttacaa taaagaagct ttttgttaga ataaatctcg tgtgtaatgg acacacgaaa
     3601 attacggcta atccgctaag acaagtactt aagattagta agataggaga ataaaaatga
     3661 atccattaat ccaaagtttg acagaaggtc aacttcgctc tgatatccct aacttccgtc
     3721 ctggtgacac tgttcgcgtt cacgcaaaag ttgtcgaagg aactcgcgaa cgtatccaga
     3781 tctttgaagg tgttgttatt tcacgtaaag gtcaagggat ctcagaaatg tacactgttc
     3841 gtaaaatctc aggtggtatc ggggtagagc gtacattccc aatccacaca ccacgtgttg
     3901 acaaaatcga agttatccgc cacggtaaag tccgtcgtgc gaaactttac tacttgcgtg
     3961 cattacaagg taaagcagct cgtatcaaag aaattcgtcg ttagtttgaa ccgaaacgta
     4021 acgtaataga ataaaaagac tagagtctct agtcttttta tgtcgtcgtg acaatgtgac
     4081 acttggtgat taacgctatg ctcgtaggac actttgaaag ttccttagca gttataatta
     4141 gtatattgta ataacaaaat gaagcaaaat aaactttcag ttatctgata tttcatcttg
     4201 cattacctta ttttctaggt taaaatagaa gacaaggtga atataagata ggccccttag
     4261 ttcaatggat ataacaactc cctcctaagg agtagttgct ggttcgattc cggcaggggt
     4321 cattattgtc actgaaaaag cctttacatc aaaggctttt tgttttattc cggttctaaa
     4381 aggggcagtt tgtttattta taatttcttt catgtttaca gttgtgtgac tgtaaataga
     4441 taatgttgtt tttggatcgc tgtgaccaac tctatccatt atcgcattta gcggtatccc
     4501 tttttctgct aaaaatgata tatgcgagtg tctaaataag tgcgtgtgat aatctccgta
     4561 aattttcaat cgcttattga tgtacgcgtt taaaattggt aaaccttttg aatatggaaa
     4621 gctgttagca ctctgcttgt gattaatcta tcgtatgtta aaagtgacat ttaaattaaa
     4681 tcatttttta tacatatatt tcaacaggat ttatcatatt ggaaaatagc ggtgcttcta
     4741 ttggcatttt actattgaca ggaatttccc tgccttttgt ctcctaaaga agatcagctt
     4801 tgatttctaa cttgattaga gtgggcttgg tactgtttat gaattatcag tgcatacttt
     4861 ctggagatca aaaaggaaag gagcagctaa agaggtgtat gtgttaccat tagaggaata
     4921 gagcatcgca agactcgttt attcagtcat tacgatacct gttttatttg ggaaataact
     4981 ataatattaa gtgaatttgt gatataatga aaggtatgaa ttatcaagat tatatatggg
     5041 atttgggagg taccttactc gataattacg agctttcaac ccaagctttt gtccaaacgc
     5101 tagctttttt cagccttcca ggagatcacg atgctgttta ccaaaaatta aaagaatcaa
     5161 ccgctatcgc tgtggcaacg tttgctccta atgagccaga atttttgcat gtgtataggc
     5221 taagagaagc agacaaattg gcacaaccca tttggtgttt gggagctaaa gaaattttgg
     5281 aaaagatagc gacttcaggt tcgcgaaatt ttttaatttc tcatcgagat tgtcaggtga
     5341 accaactttt agagcaagct gggttgttaa tctattttac agaagtcgtg acagcttcaa
     5401 atggctttgc tcggaaacca aatcctgaaa gtttgtttta tttaaaagaa aagtatgata
     5461 ttaatagtgg attagtaatt ggagatcggc taattgataa acaagcagga caagcagcgg
     5521 gttttaatac cttgcttgtt gacggtagaa aaaacctatt ggagatagta acgtagatga
     5581 ttgaagaaaa taaacatttt gaaaaaaaaa tgcaagaata cgatgccagt caaattcagg
     5641 ttctagaagg gctggaggct gtccgaatgc gtccagggat gtatattggc tcgacagcta
     5701 aagagggttt gcatcattta gtctgggaaa ttgttgacaa ctcaattgac gaagcattag
     5761 caggttttgc ctctcatatt aaagtcttta ttgaagcaga taattccatt acagtagtag
     5821 acgatggccg tggaattcca gttgacatcc aagccaagac aggacgtccc gccgttgaaa
     5881 cagtttttac agtcttacac gcaggtggta aatttggcgg aggcggctat aaggtttctg
     5941 gaggactaca tggtgtaggg tcatctgttg ttaatgcttt atcaacacaa ttagatgtgc
     6001 gtgtttataa aaacggccaa attcattacc aaaaatttaa acgcggagct gttgtagcag
     6061 atcttgagat cattggaacc actgatgtga ctggaacaac ggtacacttt acacccgatc
     6121 cagaaatttt tactgaaacg actcagtttg attacagtgt tttagcaaaa cgtattcaag
     6181 agttagcctt tttgaatcgt ggtttaaaaa tttccattac agataagcgc tcaggtatgg
     6241 aacaagaaga acatttccat tatgaaggtg gaattggttc ttatgttgaa tttttaaatg
     6301 ataaaaaaga tgttatcttt gaaacgccta tctatacaga tggtgaatta gaaggtattg
     6361 cagttgaagt agccatgcaa tacacgacta gctatcaaga aacagtcatg agttttgcta
     6421 ataatattca tactcatgaa ggtggaacgc atgaacaagg ctttagagcg gctcttactc
     6481 gggtcatcaa tgactacgct aagaaaaata aaattcttaa agaaaatgag gacaatttga
     6541 caggagaaga tgttcgtgaa ggtttgacgg cggtaatttc tgttaagcat ccaaatcctc
     6601 aatttgaagg tcaaaccaaa acaaaattgg gcaactcaga agtggttaag atcactaatc
     6661 gtctctttag tgaggccttt caacgttttc ttttggaaaa cccacaagtt gctcgtaaga
     6721 ttgtggaaaa agggattttg gcttctaaag ctagaattgc agctaagcga gcccgcgaag
     6781 tcacccgcaa aaaatcaggc ttagaaattt caaacttacc tggaaaatta gcagactgtt
     6841 cgtcaaatga tgctaaccaa aacgaacttt tcatcgtcga aggagattca gcgggtgggt
     6901 cggccaaatc aggtcgtaac cgagagtttc aagctatctt gcctattcgt ggtaaaattt
     6961 tgaacgtgga aaaagcaact atggataaga ttcttgccaa cgaagaaatt agaagtcttt
     7021 ttaccgctat gggtacaggt tttggtgcag attttgacgt gtcaaaagct cgctaccaaa
     7081 agctggttat catgaccgat gccgatgtgg atggcgctca tattagaacc ttgcttttaa
     7141 ccttgattta ccgctttatg aggcctgttc tagaagctgg ctatgtttat atcgcccagc
     7201 cacctattta tggtgttaag gtcggtagtg agattaaaga gtatattcag ccaggtattg
     7261 atcaagaaga ccaattaaaa acagctcttg aaaaatatag tattggtcgt tcaaaaccaa
     7321 ctgttcaacg ttataaaggt cttggggaaa tggatgacca tcaactttgg gaaactacta
     7381 tggatcctga aaatcgtttg atggcgcgtg tgacagttga tgatgccgca gaagcagata
     7441 aagtatttga tatgttaatg ggagatcgtg ttgaaccaag acgtgatttc attgaggaaa
     7501 atgcggttta tagtacactg gatatttagt caccttttta gtttcacaag gaatctgcta
     7561 ccaaagagga cgaattgact aaaaatcatg atgcatgatg ccaacagctt tgttagttag
     7621 tacgtcttca tcttgaggga cagcgttaat gcataggtat tgagaccgcc ccttaatacc
     7681 tatgttagtc cttattaaga aagccttttg agtcgctggt acgagattgt tgaattagtc
     7741 aaatatattt tgaaatgcat tttaaagtat gttataatcg taatgttata gtgtatttta
     7801 aataaaattt taataaaagg cgattgagga atttcagttt ttagttatta ctggaacaat
     7861 gttgataaat cgtgtctaaa taataaggag attcagatgt caagcggaat tatcttgctg
     7921 attgtggcta tagtcctact agtgattatc gcctatctag tgggtgtcat catacgaaag
     7981 cgtaatgatt ccctaattac cagtctagaa gaacgaaaac aggccttgtt tgctttgcct
     8041 gttaatgatg aaattgaaga agtaaagtca cttcatttga taggacagag ccaaacctct
     8101 tttcgggaat ggaatcagaa atgggtagac ttaactgtaa attcgtttgc agatattgaa
     8161 aatcatattt ttgaagctga aaatttgaat gatacgttta attttattcg cgccaagcat
     8221 gagattaata gcgttgaaag tcagcttaat cttgttgaag aagatatcgc ttctattcgt
     8281 gaggcactta atatcttaaa agagcaagaa gaaaaaaata gtgctcgtgt cacccatgct
     8341 cttgatttat atgaaaagct ccaagcttct atttctgaga atgaagataa ttttggttca
     8401 actatgcctg aaattgacaa acagatgaaa aacattgaaa ctgaattttc acagtttgtc
     8461 gcactcaact catcaggtga tcctgtagaa gcttctgaag ttctggatag agcagaagag
     8521 catactattg ctctagggca gattacagag cagattccag ctattgtggc caagttggaa
     8581 gatgattttc cagatcagtt agatgatttg gaaacaggat atcgccgttt acttgaagaa
     8641 aactatcatt ttccagaaaa aaacattgag gcacgttttc aggagattcg tgagtctatt
     8701 cgtgccaatt catcagagtt agttacactt gacttggatc gtgctagaga agaaaacacg
     8761 catattcaag aacgcattga ttctctttat gaagtctttg agcgcgaaat tgccgcttat
     8821 aaggtagctg ctaaaaatag taagatgcta cctcgttacc tagcacatgt gaaacgtaat
     8881 aatgaacaac tcaaagatga aattgcacgt ctatcacgta aatatattct aagtgaaacg
     8941 gaaagtctga ctgttaaggc atttgagaag gatattaaag agatcgaaga cagtacactt
     9001 gctgttgcag aacaatttgg tttacaagaa aaaccatttt cagaactgca agtgactttt
     9061 gaacgcagta taaagacgct tactaatgtc gagtcaggtc aaatggatgt ttttgcagca
     9121 gttaaagaca ttgaaaaaat tgaatcacag gcacgccata accttgatgt ttatgttact
     9181 cagcttcata tgattaaacg ttatatggaa aaacgtcact tgcctggaat tccacaagac
     9241 tttcttagtg ctttctttac aacgagttca cagttggaag ctttgatgga tgagcttagt
     9301 agaggacgga tcaatattga ggcagtgtca cgtttgttgg aagttgcgac ggttgctatt
     9361 gctaatctag aagatttaac ctatcaagtg gttcaaaatg ctaccttgac tgaacaatta
     9421 ctacaatatt ctaatcgcta tcgttctttt gaagcaggag ttcaaagtag ttttgaacat
     9481 gctttgagac ttttcgaagt agaaaatgat tatcaggctt cttttgatga aatttcttat
     9541 gctcttgaaa cagttgaacc cggggtaaca gatcggtttg ttaactctta tgaaaaaaca
     9601 cgagagcaca ttcgtttttg atacttgaaa gtcgtgatat tctcacgact ttttattagc
     9661 tttttggacc aatacgtgtt tttgactaga atcttctttt taatgatcaa aagaagaagt
     9721 agtaacagtg ttttttgcta ttgagaggat agttattttt tcaaaaatct tttccaaaag
     9781 gatttggttt tttcaggagt ttttttgcta agttgatcgt tttttgtctg ctttaagatg
     9841 acttctaaac gtgtttcctt tagatttatt gcgtggtcgg tatgaaggat aaacgcaaaa
     9901 ggtgaagtca tatgcgtttc tgtaataata gtaacaggta aatggttttc ttttgctaat
     9961 ttcatataga gtatttgaag ttgaggtgcc agcttggaag agatttttaa gtgtaaaggt
    10021 tgatagacta actgaaggct cggtaattct cttttcaaat agtcttttac tgttttagtt
    10081 tgagcttcgt ctaataaagc acttaaaacg actcgttcag catacgttcc tagatagtag
    10141 cgttgctgct caggatttaa tcttctttct ccccaggctc cttttaatag cttatcttct
    10201 aaattagtca tagatac
//
LOCUS       DAUCLR010000067         9966 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.067, whole genome shotgun sequence.
ACCESSION   DAUCLR010000067 DAUCLR010000000
VERSION     DAUCLR010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9966
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.067"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(113..1462)
                     /locus_tag="VGT87_001397"
     CDS             complement(113..1462)
                     /locus_tag="VGT87_001397"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEQ3814239.1"
                     /translation="MSHITFDYSKVLESFAGQHEIDFLQGQVTEADKLLREGTGPGSD
                     FLGWLDLPENYDKEEFARILTAAEKIKADSEVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTAKERKAPQILYAGNSISSTYLADLVEYVQDKEFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDKVKGAVKVEADANNWETFVVPDNVGGRFSVLTAVGLL
                     PIAASGADITALMEGANAARKDLSSDKISENIAYQYAAVRNVLYRKGYITEILANYEP
                     SLQYFGEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGYRNLFETVIRVDN
                     PRKNVIIPELAEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGGVPNMFVTLPAQDEF
                     TLGYTIYFFELAIAVSGYMNAVNPFDQPGVEAYKRNMFALLGKPGFEALSAELNARL"
     gene            complement(1918..2622)
                     /gene="speG"
                     /locus_tag="VGT87_001398"
     CDS             complement(1918..2622)
                     /gene="speG"
                     /locus_tag="VGT87_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptococcal pyrogenic exotoxin SpeG"
                     /protein_id="HEQ3814240.1"
                     /translation="MKTNILTIIILSCVFSYGSQLAYADENLKDLKRSLRFAYNITPC
                     DYENVEIAFVTTNSIHINTKQKRSECILYVDSIVSLGITDQFIKGDKVDVFGLPYNFS
                     QPYVDNIYGGIVKHSNQGNKSLQFVGILNQDGKETYLPSEAVRIKKKQFTLQEFDFKI
                     RKFLMEKYNIYDSESRYTSGSLFLATKDSKHYEVDLFSKDDKLLSRDSFFKRYKDNKI
                     FNSEEISHFDIYLKTY"
     gene            complement(2724..2821)
                     /gene="ffs"
                     /locus_tag="VGT87_001399"
     ncRNA           complement(2724..2821)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VGT87_001399"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            3021..4253
                     /locus_tag="VGT87_001400"
     CDS             3021..4253
                     /locus_tag="VGT87_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein"
                     /protein_id="HEQ3814241.1"
                     /translation="MGVMMKQKIKILTVIGLMTVGMSACHNTSKPSNTDSVFSLTGKK
                     RQQIVKQVRQRYYFQQLSKTEQENYLTLYDSLAQFREIISLTPASKKSLIKTIDAFVM
                     DNPEFYWITSADYRFEFSDQTVFVTFPIPEDAKNVYQDLQAIGNDIVANTPSKDRYEQ
                     VKYFYEVIIRDTDYNKKAFEAYQSGSQSQVASNQDIKSVFIDHLSVCNGYAQAFQFLC
                     QKAGIPVAYIRGTGTSQQPQQSFAHAWNAVQINNTYYGVDVTWGDPVFDNHLSHQKQG
                     TINYSFLCLPDYLMALSHQPSKDIAFNTKERFENVWTIPSCTDDSLLYSKRHQSYIST
                     FDSDAILASLENQLLNRQEQLSLQFAHQDDYQQMVTDLTTNQTGYHNLFNQYWNNYTG
                     FTYGLLPETLSISFASRN"
     gene            complement(4867..5382)
                     /locus_tag="VGT87_001401"
     CDS             complement(4867..5382)
                     /locus_tag="VGT87_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEQ3814242.1"
                     /translation="MPYSLEEQTYFMQEALKEAEKSLQKAEIPIGCVIVKDGEIIGRG
                     HNAREESNQAIMHAEMMAINEANAHEGNWRLLDTTLFVTIEPCVMCSGAIGLARIPHV
                     IYGASNQKFGGADSLYQILTDERLNHRVQVERGLLAADCANIMQTFFRQGRERKKIAK
                     HLIKEQSDPFD"
     gene            complement(5382..6161)
                     /locus_tag="VGT87_001402"
     CDS             complement(5382..6161)
                     /locus_tag="VGT87_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEQ3814243.1"
                     /translation="MKLTTLGSWGAYPYQDAGTTSYLVTGHDGFQLLMDAGSRALNEL
                     EKEISPLDLDAVIISHYHPDHVADLGVLRHYFQLYPKHLWQPKVLPIYGHDEDRHEFA
                     KLSIPDVSEGRAYDVTGVEQIGPFDITFLKTVHPVVCYAFRIVERATGQVLVFTGDSG
                     YFEGLADFAKEADLFLADVYLYEGNENHMAHLTSKEAGLMASQARVKKLVLTHMPPVP
                     PEGINSKNHLEVLRQETQAYAGNIPVDLALPHKSWNLGSLS"
     gene            complement(6301..6840)
                     /locus_tag="VGT87_001403"
     CDS             complement(6301..6840)
                     /locus_tag="VGT87_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEQ3814244.1"
                     /translation="MFTTKELVKVAMMTTLIIILGFIPAIPLGFIPVPIVLQNLGVML
                     AGLMLGGKKGTLSVFLFLVIGLFLPVFSGSRTTIPVLMGPSAGYVIAYLLVPIVFSLL
                     YRNWFSKSTPLAFLALLISGVVLVDVLGAIWLAAYTGMSLVTSLLSNLVFIPGDTIKA
                     IIATIIAVKYKDSFLNTKQ"
     gene            complement(6844..7155)
                     /locus_tag="VGT87_001404"
     CDS             complement(6844..7155)
                     /locus_tag="VGT87_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814245.1"
                     /translation="MTDCFGIDLDQEYRCLHYHTPLDIVGLKCAFCQTYYACYHCHDQ
                     LTDHAFVPTGHQEISPVICGHCRKLLSLAEYGCGCCPYCQSPFNPACHRHKDIYFLKE
                     S"
     gene            complement(7372..8514)
                     /gene="tgt"
                     /locus_tag="VGT87_001405"
     CDS             complement(7372..8514)
                     /gene="tgt"
                     /locus_tag="VGT87_001405"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEQ3814246.1"
                     /translation="MTDYPIKYRLIKTEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKAIGSGIILSNTYHLWLRPGDELIARSGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAADLVAMDFPGYSI
                     GGLAVGESHEEMNAVLDFTTPLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSEGRLVVKNAKFAEDFTPLDHDCDCYTCQNYSRAYIRHLLKADETFGIR
                     LTSYHNLYFLVNLMKKVRQAIMDDNLLEFRQDFLERYGYNKSNRNF"
     gene            8733..9596
                     /locus_tag="VGT87_001406"
     CDS             8733..9596
                     /locus_tag="VGT87_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEQ3814247.1"
                     /translation="MSIKAIKGQARDTLKNLSGKYLLFLIPTLLFMFHFGIEIHQGYV
                     LSSGIEVSLAASYFPLLLGVILSLFILSASFTMIDVVRHFRQKVSFAESTTAFSKEFF
                     ANLLVLAITKWLFFLIWSLIWFFGLFIFLSGLSAFLVNAKSGSSTVISLIFLLFGAVL
                     SLIGFGIYINRYYAYSLSEYLLYDKVKEGTYLGAIAVIKTSVAMMKGYKWKLFFLQLS
                     FTGWFLLNIVTFGLLNIYLLPYFTTANVIFYDQLKKRFKDKDDPIEGEHLSLHQLNKK
                     PTPMMYDDQKD"
     gene            9793..>9966
                     /locus_tag="VGT87_001407"
     CDS             9793..>9966
                     /locus_tag="VGT87_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEQ3814248.1"
                     /translation="MIRLIDRYGVTIVQKCKNNYYSPELKQEIINKVLIDGQSQKQTS
                     LDYALPTSSMLSRW"
BASE COUNT         3112 a         2024 c         1778 g         3052 t
ORIGIN      
        1 tttacttaac cttgtatcca ctcgatgatg gaagtgccgc cccaattctt agattgcttg
       61 gctaactttt aagctcttgt acaaggaaga agtctttttg actatctgtt gattagaggc
      121 gagcattaag ctctgctgat aatgcttcaa aacctggttt tccaagaagc gcaaacatgt
      181 tacgtttgta agcttcaaca cctggttgat caaatgggtt gacagcattc atgtagcctg
      241 aaacagcgat ggcaagctca aagaagtaaa tagtgtaacc aagggtaaac tcgtcttgag
      301 ctggaagagt gacaaacatg tttggcacgc cgccatctgt gtgggcaaga agcacaccat
      361 ctgtagcttt cttgttaaca aaatcaacgt ctttaccttg aaggtaacca agaccatcta
      421 ggtcttctgc taattcagga atgataacat ttttacgagg attatcaaca cgaataactg
      481 tttcaaagag attacggtaa ccttcttgga taaattgacc aagtgagtga aggtctgttg
      541 agaagttagc tgaagttggg taaatccctt tttggtcttt tccttctgat tcaccagcta
      601 attgtttcca ccattcgcca aagtactgaa gagatggctc gtagttagcc aagatttcag
      661 tgatgtagcc tttacggtaa agcacattac gaacagcagc gtattggtaa gcaatgtttt
      721 cagagatttt gtctgaagaa aggtctttac gagcagcatt tgccccttcc ataagagcag
      781 taatatctgc acctgatgca gcaattggaa gcaaaccaac agcagtcaag actgagaaac
      841 gaccaccaac attatctggc accacaaatg tttcccagtt gttagcgtca gcttctacct
      901 taacagcacc cttaactttg tctgtggtag cgtagatacg tttgttagct tcttcttggc
      961 catatttttt aaccaaaagt tctttgaaca cgcgaaaggc aatagctggt tcagtggttg
     1021 tacctgattt agagatcacg ttgactgaga actctttgtc ttgaacgtac tcaactaaat
     1081 cggcaaggta agtagatgaa atagagtttc cagcgtaaag aatttgtggg gctttgcgct
     1141 ctttggcagt ttgcaagtta gcaaaatgat gatttaaaaa atcaatcgct gctttggcac
     1201 caaggtatga cccaccgata ccgatcacca caagaacttc gctgtctgcc ttaatctttt
     1261 cagcagcagt caagatacgt gcaaattcct ctttgtcata gttttctgga agatcaagcc
     1321 agcccaagaa atctgaacct ggtccagttc cttcacgcaa taacttgtca gcttctgtta
     1381 cttgaccttg taaaaaatca atttcatgtt gtcccgcaaa tgactcgagg acttttgaat
     1441 aatcaaatgt aatatgtgac atgagtttcg ctcccttaaa attttaagtt tcattagtat
     1501 gataaccttt tctatctaat atttcaatgg tttttagtgg ttttcacctg tgaaaaagct
     1561 aggttttttg aaaaggtttt cgttttttca gacataaaaa atgtgtcaat gcaagcatat
     1621 tctcatcata gtggtttttt aagagaaaat aggatttcat atttcctaaa aatatcatga
     1681 aaaaaggctc tgaggccgct ttaccttaat aacttatttt aaggatttta tcatttcaat
     1741 acgggtaata accccatcat gcatatgatc aatgcataag gccacacccg caaaggcaat
     1801 tgaagcccca atcacctgta aatactttct catgctatct tcttaacatt acttactctc
     1861 attatctaaa aatggcaatt ctttgaatac ttaaaaagca aaggggggag aatagcacta
     1921 gtacgttttt aagtagatat caaaatgact aatttcttca ctattaaaaa tcttattatc
     1981 tttatacctt ttaaagaaac tgtctcgact taaaagctta tcatccttac taaataaatc
     2041 aacttcataa tgtttactat ctttagtagc aaggaaaagg ctccccgatg tataacgcga
     2101 ttccgaatca tagatattgt atttttccat tagaaatttt cttattttaa aatcaaattc
     2161 ctgtaaagta aactgtttct tttttatgcg aacagcctca gagggcaaat aagtttcttt
     2221 cccatcttga tttaaaattc ctacaaactg taatgattta tttccttgat tcgaatgttt
     2281 tacaatacca ccataaatat tatctacata aggttgggaa aaattataag ggagaccaaa
     2341 aacatcgacc ttatcccctt ttataaactg atcagtaatg cctaaagata caatagaatc
     2401 aacataaaga atacattccg atcttttttg tttagtatta atatgtatgc tatttgtagt
     2461 aacaaatgca atttctacat tttcataatc gcatggggta atattatagg caaaccttaa
     2521 acttcttttt aaatctttta aattttcatc tgcataagct aattgacttc catagctaaa
     2581 aacacatgat aatatgataa ttgtcaaaat gtttgttttc atttaaaact ttttatgtcc
     2641 tgttttatac ctcttaacct aaataaaaat atcaatcggt ttcaacaact gatgacacta
     2701 tagaaggagg agattacttt agtaaagtca acaaaaaaga gcacacaccc gaaaatcgct
     2761 tagggctgct ggattcctcc cctgacccac ttcacgcaca agtgttgctc cgcctattat
     2821 tatatcatat tacggacagt ttgcaagttt tccaactaag taaaatcact ttcaagtgag
     2881 aatagcttca ttttgggaca agatggactg aatacctaga aaaatcatga ccctttccaa
     2941 acacgatttt gctttttgaa aatctttccc tctccccatt ccagctattc tctatgttat
     3001 aataagatta ttagattaga atgggagtta tgatgaaaca aaagattaaa atcttaacag
     3061 taattggcct tatgacagtt ggtatgtctg cgtgtcacaa cactagcaaa ccttctaata
     3121 ccgattctgt tttttcttta accggaaaaa agcgccaaca aatcgtcaaa caagtaagac
     3181 aacggtatta tttccaacaa ttatccaaaa ccgaacaaga aaattacctc actttatacg
     3241 atagtctagc acaatttcgt gaaatcatca gtttgacgcc ggcttctaaa aaaagcttaa
     3301 ttaaaacgat tgacgccttt gtgatggata atcccgaatt ttactggatt acatctgccg
     3361 attatcgatt tgagttttct gatcaaacgg tctttgtcac ctttcctatc ccagaagatg
     3421 ccaagaacgt ttatcaagac ttgcaagcaa tcggtaatga tatcgtcgca aacacgccat
     3481 caaaggatcg ctatgagcag gtcaaatatt tttatgaggt catcattcga gacacagact
     3541 acaataaaaa agcctttgaa gcctatcaat caggcagtca atcccaggtc gcttctaatc
     3601 aagacattaa aagtgttttt attgatcatt tatctgtttg taatggctat gcccaagcct
     3661 ttcagtttct ttgtcaaaaa gctggcattc cagtcgccta tatccgtgga actggtacat
     3721 ctcagcaacc tcagcaatct tttgcacatg catggaatgc tgttcaaatt aataacactt
     3781 attatggcgt tgatgtcact tggggtgacc ctgtttttga taaccatctc tcacatcaaa
     3841 agcaaggaac tatcaattac agttttctat gcctaccaga ttatctaatg gccttatcac
     3901 atcagccaag caaagacatt gcttttaata ctaaagaacg ttttgaaaat gtttggacta
     3961 taccttcttg tacagatgat tctttgcttt attctaaacg tcatcaaagc tatatctcaa
     4021 cgtttgacag tgacgccatc cttgcaagcc ttgaaaatca gttgttaaat agacaagagc
     4081 aactgtctct acaatttgct catcaagatg attatcaaca aatggtaact gatttaacga
     4141 cgaatcaaac aggttatcat aaccttttca atcagtattg gaataattat acgggcttta
     4201 cctatggact cttaccagag actcttagta tttcatttgc tagtaggaac taggtttaga
     4261 cacatgaaac acaaagttaa gatgattatc tttgtcaaac attacaagaa attgatatac
     4321 taacacaatt gaaggcgatt attattctaa ccgtcttcaa tagatataac cttttcagat
     4381 tgagcacatc taatgcttgt aacattagca acagtgatga gctctttttt aatgctttag
     4441 caaaatatat cagctaagac aaaagattaa ataactcaag tcatgcctat aagtcacttg
     4501 tttttaatcc ctgtgtcagt gtcaatctac gccacagata actgacagct ctgagcccta
     4561 gaagtctgaa cttgataagt ttactaaaaa caaaaataag gtgagccatt actatctgtt
     4621 tttagatagt aaatcttttt aagaatgact aactcatcta gtcatcacgt gttttatttt
     4681 tcgattgttt tcatctcgat tgggtctgaa aaagagcgct tctagtactt ggaaacgccc
     4741 ttttgtatat tctatcttcg tatgttgaat ctaacaacca ttggaaaaat ggtctgacgt
     4801 ttcactcagt attttgacag gctacccttc accaaaaatg catgtgataa caaactcctt
     4861 aaaactctag tcaaagggat ctgactgttc cttgatcaaa tgcttagcta tttttttgcg
     4921 ctctcgccct tggcggaaaa aggtttgcat gatgttagca cagtctgctg ctagcaggcc
     4981 tcgttcaact tgcacgcggt ggtttaagcg ctcatctgtc aaaatctggt aaaggctgtc
     5041 tgctccacca aatttttgat tgctggctcc ataaataaca tgcggaatcc ttgctagccc
     5101 aatggcacca ctacacatca cacaaggctc gatggtcaca aagagtgtgg tgtctaataa
     5161 acgccagttg ccttcatgag cgttagcctc gttaatagcc atcatttcag cgtgcataat
     5221 ggcttggtta ctctcttcac gcgcattgtg cccacgacca ataatctcac catccttgac
     5281 aatgacacag ccaatgggga tttcagcttt ctgtaaggat ttttcggctt cttttaaagc
     5341 ctcttgcatg aaataagttt gctcttctaa actatatggc atcaagaaag actccccaag
     5401 ttccaagatt tgtgtggtaa ggcaaggtca acaggaatat tacctgcata ggcctgtgtt
     5461 tcttgcctca acacttctaa atggtttttg gaattaatac cttctggagg aaccggtggc
     5521 atgtgggtca agacaagctt cttaacacgt gcctggctag ccattagtcc tgcttcctta
     5581 ctggtcaaat gggccatatg attttcattg ccttcgtata agtaaacatc cgctaagaaa
     5641 aggtctgctt cttttgcgaa gtctgccaat ccttcaaaat aaccactgtc tccagtaaag
     5701 acaagtacct gaccagtcgc tctttctaca attcgaaagg cataacacac aactggatga
     5761 actgttttaa gaaaggtaat gtcaaaagga ccaatctgtt caactccagt aacatcatag
     5821 gctctacctt ctgaaacatc aggaatgctt aacttagcaa attcgtgtcg gtcctcatca
     5881 tgcccataaa tgggcaatac tttaggctgc cacaggtgtt taggataaag ttgaaaataa
     5941 tggcgcaaca cgcctaaatc ggccacgtgg tctggatggt aatggctaat aatcactgca
     6001 tccaaatcga gaggactgat ttctttttcc agctcattga gcgctcgact accagcatcc
     6061 atcaataact gaaagccgtc atgtcctgtg accaaataag aggttgtgcc tgcatcttga
     6121 tagggatagg ctccccaaga ccctaaggtg gttaatttca tctcaaaccc ttctttcttg
     6181 ctttaactct tattataaca aaaattagtg tcaaagagga ttgagaccca ccaaaaacag
     6241 gtcctagcag ataaatctgc caagacctgt tcaacacaac ttaactattt gatgattaga
     6301 ttactgtttt gtattcaaaa aactatcttt gtatttaaca gcgataatgg tcgcaatgat
     6361 tgctttgatg gtatctcctg gaataaagac caagtttgac aaaagcgatg tcacaagaga
     6421 catgccagtg taggctgcca accagatagc acccagaaca tcaaccagta caactcctga
     6481 gattaaaaga gctaggaatg ctaatggcgt gctttttgag aaccagttgc ggtaaagtaa
     6541 tgaaaagaca ataggcacaa gaaggtaggc aataacatag ccagcagatg gccccatcaa
     6601 tactggaatg gttgttcttg agcctgagaa aacaggtaag aaaaggccaa tcactaaaaa
     6661 taagaaaaca gataaggttc cctttttacc acccagcatc aatccagcca acatgacacc
     6721 taagttttgt aaaacaatag ggactgggat aaagcctagc gggatagcag gaataaagcc
     6781 taaaataata atcaaagtgg tcatcatagc caccttaaca agttctttgg ttgtaaacat
     6841 cagttacgac tcctttaaaa aatagatatc cttgtgccta tgacaggctg gattaaaggg
     6901 actttgacaa taaggacagc atccacaacc atactcagct aggctcaaga gctttcgaca
     6961 atgcccacaa ataacaggag atatttcttg atgtcctgtt ggcacaaagg catgatctgt
     7021 taactggtca tggcaatggt aacaagcata ataagtctga caaaaagcac atttgagccc
     7081 aacaatatca agcggggtat gataatgtag gcaccgatac tcttggtcaa ggtcaattcc
     7141 aaaacagtcc gtcatatctc atcctcttgt aatagtttaa tcaaaagctt atagtttgtc
     7201 aactctattt ttacaaaagg gtaactgctt tctgaaaaag aacacgtaaa acaagaactt
     7261 ccctttaaag agtgacctat cagttgaaag caataggaca tccgtctata caaaaaaact
     7321 gaaaccaaag acgatagatc gccctcagtt ccaggatcat agcattttta attagaaatt
     7381 acggtttgat ttattataac cataccgttc aagaaaatct tggcggaatt caagtaagtt
     7441 atcgtccata atagcctgac gtactttttt catcaagtta accaagaaat aaaggttgtg
     7501 gtagcttgtc aaacgaatac caaaagtttc atccgctttt agcaagtgac gaatgtaggc
     7561 acggctatag ttttgacaag tgtagcagtc acagtcatga tcaagtgggg taaaatcttc
     7621 agcgaatttg gcatttttaa ccacaagacg cccctcacta gtcatgcagg tcccattacg
     7681 agcgatacgc gttggcaaga cacaatcaaa catatcgaca ccacgaatca caccatcaat
     7741 caagctatct ggtgctccca ctcccatcag gtaacgaggt ttattttctg gtaagagggg
     7801 agtcgtaaag tcaaggacgg cattcatttc ttcatggctc tccccaacag ctaaaccacc
     7861 aatagaataa cctgggaaat ccatagccac caaatctgcc gcagactgac gacgaaggtc
     7921 ctcaaatcca gctccttgca caattccaaa taatccttgg tcatgaggac gacgatgggc
     7981 ttttaggcca cgttctgccc aacggctagt acgttcaatg gattttttca cgtaatcata
     8041 aggttggtaa aattgcgggc actcgtcaaa actcatcatg atgtctgagc caaggttatt
     8101 ctgaatggaa atggcttttt cgggtgatag aaacattttt gagccattca agtgattttt
     8161 gaaagtcact ccttcttcag tgatgtttcg tgaatcggct aaagagtaaa cttgaaaacc
     8221 acccgaatcg gttaagattg gctgatccca gttcataaac ttgtgcagac caccagagcg
     8281 agcaatcaac tcatctcctg gacgcaacca gaggtgataa gtgtttgata aaataatccc
     8341 tgacccaata gcttttaact cttctggaga ctgtgtttta acagtagcct gagttcccac
     8401 tggcataaac attggtgtcg gaaaggtccc gtgaggtgtg ataatttcgc ccaagcgtgc
     8461 accggtgtgc ttttctgttt tgataaggcg atacttaatc ggataatctg tcatgtgctt
     8521 tcttccttgc tatcaagata aacagtcttg agcttttttg atctgcctga gtttccatca
     8581 taatgaaggg cttcaagatg acatgttgtt ctagcgccta tcattttatc aaaaaatcca
     8641 taatttgtca tgctttccca aaaaagtata gtcaaaataa tggttagctg tctcattttg
     8701 ttttatggta taatgaaata aaggagtctg ttatgtcaat taaagcaatc aaaggccagg
     8761 ctagagacac gttaaaaaat ctctctggca agtatttact atttttaatt ccaacccttc
     8821 tctttatgtt tcatttcggt attgagattc atcaaggtta cgtcctatct agtggtattg
     8881 aagtctcgct agcggctagt tatttcccat tactactagg agttatcctt agccttttca
     8941 ttttatcagc atcctttaca atgattgatg ttgttaggca ttttcgtcag aaagtttctt
     9001 ttgccgaatc aacaacagcc ttctcaaaag aattctttgc gaacctgctg gttttagcga
     9061 tcactaagtg gctattcttt ctcatctggt cactaatttg gttttttggc ctctttattt
     9121 tcctttccgg actttcagcg tttttggtca atgctaaatc aggtagctca acagtcattt
     9181 cactgatctt tttactattt ggagcagttt tgtccttaat tggttttggt atttatatca
     9241 accgttatta cgcttatagt ctatcagaat accttctcta cgataaggtt aaagagggga
     9301 cttatctagg agctatagcc gtcattaaaa caagtgtagc tatgatgaaa ggttataaat
     9361 ggaagctttt tttcctacaa cttagcttca ccggttggtt tctactgaat attgttactt
     9421 ttgggttact taatatctat cttttaccat attttacaac agccaatgtc attttttatg
     9481 atcaactcaa gaaacgtttc aaagacaaag atgacccaat tgaaggagaa catttaagcc
     9541 ttcatcagct taataaaaag cctactccta tgatgtatga cgatcaaaaa gactaaataa
     9601 atcctccgtc cataaaaata gcccttgaac tgcaccccaa aagttagaca caaagtctaa
     9661 cgattggggt gctatttttt tatgaaattt aatcaagaaa cgaaagttaa gatttatgag
     9721 ttacggcaaa tgggagagtc cattaaatcc atatcaaaaa agtttgatat ggcagaatcc
     9781 gatctcaaat atatgattcg cttgattgat aggtatgggg taaccattgt tcaaaaatgt
     9841 aagaataatt attattctcc agaactgaag caagagataa taaataaagt cctgattgac
     9901 ggccaatctc agaaacaaac atccttagat tatgctttac caacttctag tatgctctca
     9961 aggtgg
//
LOCUS       DAUCLR010000068         9841 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.068, whole genome shotgun sequence.
ACCESSION   DAUCLR010000068 DAUCLR010000000
VERSION     DAUCLR010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9841
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.068"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            110..1417
                     /gene="eno"
                     /locus_tag="VGT87_001408"
     CDS             110..1417
                     /gene="eno"
                     /locus_tag="VGT87_001408"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEQ3814249.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYLGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAVLGVSIAVARAAADYLEVPLYTYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMIMPVGAPTFKEGLRWGAEVFHALKKILKERGLVTAVGDEGGFAPKFEGTEDG
                     VETILKAIEAAGYEAGENGIMIGFDCASSEFYDKERGVYDYTKFEGEGAAVRTSAEQI
                     DYLEELVNKYPIITIEDGMDENDWDGWKALTERLGGRVQLVGDDFFVTNTEYLARGIK
                     ENAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAG
                     QIKTGSLSRTDRIAKYNQLLRIEDQLGEVAQYKGIKSFYNLKK"
     gene            complement(1899..2928)
                     /locus_tag="VGT87_001409"
                     /pseudo
     CDS             complement(1899..2928)
                     /locus_tag="VGT87_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014677767.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3195..4736)
                     /locus_tag="VGT87_001410"
     CDS             complement(3195..4736)
                     /locus_tag="VGT87_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ3814250.1"
                     /translation="MLHLHLETKLQDKLSLLNILLDVSEVSIDQLCQETELKKQRVYN
                     LLFEMIKDLEDTLTLTICDDTVSIPYKTYQLKMPYFKKLYQTSIFLKILCFLIEPGEL
                     SLHDFIKREYISQATAYRIRTNCRKYLKKVGLNVRQNHVVGPKYRIRFLIALLHYQFG
                     MTIYDFDKTSMNKVVSLIINSNQAITLNDASKAPYEFSYFAILISLIWKRRHDNLGIP
                     QTDAFKHLKKLSIYHDIKMTSQEIIGKWYHPDLTDEDLDYIFLCFCTTNNPFHKDKWT
                     PKKVKELFELVMTKTIGKTLKASLRPLLGNNILKSLPFKRILVSFSRLFISNLQVLLP
                     DIHLFHYLRRQQKRNKSFYNTLKTIVEEWMSAEGIVGKLPSYHLLLFTIQLEELLKTY
                     LPPIPVYLLTNNTAALDLMTNALSIYFPPAIATVMPVNVEIIPFKDIVKEKQSVIIAD
                     RQYLNLIQHLYQNQGHLFLYFLFSFRDVSEAYIHKAFLDFRQKRYDEFIVTLLDTYHK
                     NLSSP"
     gene            6135..>9841
                     /locus_tag="VGT87_001411"
     CDS             6135..>9841
                     /locus_tag="VGT87_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEQ3814251.1"
                     /translation="MEVYKNGQLLKKETVTTAGNKVNIALSDDVVLKKDDEITFKVKQ
                     GSSGKKNQAAGKIIVQQNPAVVAEQENSKKSLEEVYNKAKKAISDDEWLSDEEKRVQT
                     AAVEKTYNEGQTSINNAETVTEIKDAFKKYSTQGNKISIPDQYKKGEKAIKQEQARKS
                     LKQVHEDTLGKIRSDDWLTEEEKATQTENAKQSYNQGENKINQSDSLKTLNQILKDYT
                     SEEPNKKESIPNKYEKGKKEELKRDAETKLREAQETTKQAIENDPWLSPEQKKAQKEN
                     AKTRLDAGLKAVETTESLDKLKEVESDFLDKEKAESIPSQHQAGTPEVARKTFLDNFE
                     KEAKKEIESIKNDATLTDAEKATAKAKVEAQLQEAKDKAKESKSFDDLKNIQDKFNSE
                     LPHTTGKPLKDQQSDAIAELEKKQQEIEKAISEDKTLSKDEKEKQIADSKAKLVAEKE
                     KVSKAPDADAVKKALELGKQEIAKAYVPQNLEDHKKKLLAELKQKANDTEKAIDFDKT
                     LTAKEKEEQKAKTKEELQKATEAVGAIDNREELDKKVPELKKAIQDSHVKGDLEGVKN
                     KAIEDLQKVHDETVAKINGDDTLDKATKEAQVKEADKALAAGKDAITKADDADKVGAA
                     VTEHTPKIKAAHKTGDLKKVQEEANQALDKAAEKERGEINNDITLTAKDKEQQLKEVE
                     TALTKAKAKVAEAKTADAINDARDKGVATIDAVHKAGQDLGARKSGQVAKLEEAAKAT
                     KDKISADPTLTSKEKEEQSKAVNAELKKAIEAVNAADTADKVDDALGKGVTDIKNQHK
                     TGDPVVARREAHGKQLDRVAQETKDAIEKDPTLTTEEKAKQVKDVDAAKERGMAKLTE
                     AKNADELDKAYGEGVTDIKNQHKSGDPIEARRGLHNKSIDEVAQATKDAITADTTLTE
                     AEKETQRGNVDKEATKAKEELAKAKDADALDKAYGDGVTSIKNQHKSGKGLDVRKDEH
                     KKALEAVAKRVTAEIEADPTLTPEVREQQKAEVQKELELATDKIAEAKDADEADKAYG
                     DGVTAIENAHVIGKGIEARKDLAKKDLAEAAAKTKALIIEDKTLTDDQRKEQLLGVDT
                     EYAKGIENIDAAKDAAGVDKAYSDGVRDILAQYKEGQNLNDRRNAAKEFLLKEADKVT
                     KLINDDPTLTHDQKVDQINKVEQAKLDAIKSVDDAQTADAINDALGKGIENINNQYQH
                     GDGVDVRKATAKGDLEKEAAKVKALITNDPT"
BASE COUNT         3483 a         1665 c         2065 g         2628 t
ORIGIN      
        1 caaaaaacaa aggaagcttt tcatgagatt gctttttaag ttgattaaag gcctagtttg
       61 tggtattatg aacatgtaaa atattaaaac tcataaggag agtattacaa tgtcaattat
      121 tactgatgta tacgctcgcg aagtccttga ctcacgcggt aacccaacac ttgaagtaga
      181 agtttataca gaatcaggtg cattcggacg tggtatggtt ccttcaggag cttcaactgg
      241 tgaacatgaa gccgttgaac ttcgtgatgg tgacaaatct cgttaccttg gtctaggtac
      301 tcaaaaagca gttgataacg ttaataacat tatcgctgag gcaatcatcg gttacgatgt
      361 tcgcgatcaa caagctatcg accgtgcaat gatcgcactt gacggtactc ctaacaaagg
      421 taaacttggt gctaacgctg ttcttggtgt ttctatcgct gttgctcgtg ctgctgctga
      481 ctaccttgaa gtgccacttt acacttacct tggtggattc aacactaaag ttcttccaac
      541 tcctatgatg aacatcatca acggtggatc acactcagat gctccaattg cattccaaga
      601 gttcatgatc atgccagttg gtgcacctac tttcaaagaa ggtcttcgtt ggggtgctga
      661 agttttccac gctcttaaga aaatccttaa agaacgcgga cttgttacag ctgtaggtga
      721 cgaaggtgga tttgctccta aatttgaagg aactgaagac ggtgtagaaa ctatccttaa
      781 agctattgaa gcagctggtt acgaagctgg tgaaaacggc attatgatcg gtttcgactg
      841 tgcatcatca gaattctacg acaaagagcg tggcgtttac gactacacta aatttgaagg
      901 tgaaggcgct gctgttcgta catctgcaga acaaatcgac taccttgaag agttggttaa
      961 caaataccca attattacta tcgaagatgg tatggacgaa aacgactggg atggttggaa
     1021 agctcttact gaacgcctag gcggacgtgt tcaattggtt ggtgacgact tcttcgttac
     1081 aaatactgaa taccttgctc gtggtatcaa agaaaatgca gctaactcaa tccttatcaa
     1141 agttaaccaa atcggtactt tgactgaaac ttttgaagct atcgaaatgg ctaaagaagc
     1201 tggttacact gccgttgtat cacaccgttc aggtgaaact gaagattcaa caatcgctga
     1261 catcgcagtt gcaacaaacg ctggccaaat caaaacaggt tcattgtcac gtacagaccg
     1321 tattgctaaa tacaaccaat tgcttcgtat cgaagatcaa cttggtgaag ttgctcaata
     1381 caaaggaatc aaatcattct ataacttaaa aaaatagacg gtaagacaga aatcggtcat
     1441 tcgtagaatg cgatttcgtc gtcgcacctc cgtcaggctg actaggttgg tcacgacaaa
     1501 aggagtggac atccagtcag ctacgacgca aaagttaaag attaaagaat agaaagcttg
     1561 ggaaaatccc aagctttttg atttctaaat agtgaaaatt tactgtggac taatttatcc
     1621 tgagctaatg tattagcgag gtaaagttat tgaggaaggc ttggatttgt actgccccca
     1681 aacagttgga cactatattt taaatacaga actaagtcct tttataccaa ttgtcaagca
     1741 catcaaggtt ttaactgaaa aaatgtttta gacaatagcc taggtttagc tgttgtctta
     1801 cttgttgtgc tctgattagc ataaaaaagt gtacaagaaa aaaaccttgc ctagtatttt
     1861 taagatcaag gtgttacaat gatagagcaa caacgacttt aatgattttg ggtcgaagcg
     1921 taattgtaaa gtttgttatg cgttatgagg taaagcattg tcctgacaag gcaatgtcta
     1981 gaggcaatcg tatgtggctt ggttgaagct gtttgcgatt gcctttttcg tttctcatag
     2041 aagtctgcga tatgacaagg attggtatga ctagcagaag caatgtcatg gatacacttg
     2101 aaaagaatct ttggagcata aggattccaa cgtttggtaa tgtgctcttg agctagatag
     2161 ttgccagact cataatgtct aaagtcaatc ccaatgaaag cattaatttg attagcagat
     2221 tgaaaacgac gaatagcacc gagttcgcca ataatacttg ttgcagttgt ttctgcgata
     2281 cttggaatag agcgtagaat cttgtcttct ggtaaaggtt gagccaacgc caccatctta
     2341 tcaaaaatag cttgacgacg ttcagaaagt ctaagtaatt ctttggcata gcaacgaacc
     2401 tcttctatca tcggagaggt tttcgtaaca gtaaaataag actgcttggc taatgagaca
     2461 agtttttcca ctaagtagat cccacacttg tcagagatac gtttagaggt agattgacgg
     2521 atgatagctg acaatgcttt gtcagctagc tctagtacaa agctattaca tggaaaagcc
     2581 gttaccacat tccagtatta ctcaccagtt ggtgcaaata agatgtgttc cagttcagga
     2641 aaagtgactt gtaagatctt gtgaaggcga ttcttaactc tcgaacaata ttatcctcag
     2701 tcaatttttg atagaagcga ctcaagtcac gtagcagctg ataaacttct tcctgaacgt
     2761 aagttggttt tctaactaga ataaattgag actgagccaa tttttcagtg tctaacttat
     2821 ccgctttacg cacccgtaga ctatctagtt ttctaaagga ttgagttgtg tataagcgta
     2881 gccatttttc tcaaggaagg actgaagccg acgagaatag actcctgttg tgatggtctc
     2941 ccttttgcct tttaaacaat agtatagccg tttttcaaga tgttattaat gattttttcc
     3001 tttattctag aagaataagt ctgatactgt ttcttcttaa taatctcaat gccctaacag
     3061 tccatcagtc gaacggtatt tctcaaatta ctgcagttga ctcttgcgtt cagttccctc
     3121 accttgtgga tgccgtttag aacaatatta gcttgctgag ccttttggta atgtgaacaa
     3181 agcatcttta tcactcaagg agaagacagg ttcttgtggt aagtgtccaa caaggtcacg
     3241 ataaactcat cgtaccgttt ttggcggaag tctagaaatg ccttatgaat gtaagcctca
     3301 gaaacgtctc taaaactaaa caagaaatac aagaacaaat gcccttgatt ctggtacaaa
     3361 tgctgtatca ggttcaggta ttgcctatct gcgataatga cactctgctt ttccttaaca
     3421 atatctttga aaggaatgat ttcgacattg acaggcataa ccgttgcaat agctggcggg
     3481 aagtaaatcg agagtgcatt ggtcatcaaa tccagcgcag cagtgttatt ggtcaaaaga
     3541 tagacaggta tcggtggcaa atatgttttt aggagttcct ctaactgtat cgtgaagagc
     3601 agtaaatgat aggacggcag tttccccacg atgccttctg cgctcatcca ttcctcaacg
     3661 atggttttta gagtattgta gaagctcttg tttcgttttt gctgtcttct gaggtaatgg
     3721 aacaagtgta tgtcaggtaa caaaacttga agattactga taaagagacg tgagaaggaa
     3781 accaaaatcc gtttgaaagg aagggatttt aggatattat tgcctagtag tggacgcagg
     3841 gatgctttca aggtttttcc aattgttttt gtcatcacca actcaaacaa ttctttcact
     3901 tttttaggtg tccatttatc cttatggaaa ggattattgg tggtgcaaaa acataagaaa
     3961 atataatcca aatcctcatc agttaaatca ggatggtacc attttccgat gatttcctga
     4021 ctggtcattt tgatatcgtg atagatagac agttttttca agtgtttgaa tgcatctgtc
     4081 tgagggatcc ccaagttatc atgtcgccgt ttccatataa gggatatcag aatggcaaag
     4141 tacgaaaatt cataaggggc tttactggca tcattaagtg tgatggcttg attggagttt
     4201 ataatcagcg aaaccacttt attcattgat gttttatcaa aatcataaat ggtcatacca
     4261 aactgatagt gcaacaaggc gatcagaaag cggatacggt attttggtcc aacaacatgg
     4321 ttttgccgaa cgtttaaacc tacctttttc aagtatttcc gacaattggt cctgatacgg
     4381 taagcggtgg cttgggaaat atactctcgt ttaatgaagt cgtgtaggct cagctctcct
     4441 ggttcaatta aaaaacataa tatctttaag aatatcgagg tttgatagag tttcttaaaa
     4501 taaggcattt taagttggta ggtcttatag gggatagaaa cagtgtcgtc acaaatggtt
     4561 aaggtgaggg tgtcttctaa atccttaatc atttcaaaga gtaagttgta gacacgttgt
     4621 tttttgagtt ctgtttcctg acacaactgg tctatggaga cttcggaaac gtctaacaaa
     4681 atgttgagca gtgataattt atcttgtaat ttggtttcta gatgaaggtg taacataact
     4741 gaactccttt catttttata gcagaaactc gtatttattt aattatcaca taattaaata
     4801 aaacagtcaa ataattcaat ggtgacgcgt gtttaaggag tattgagaat gtcagatata
     4861 agcaaaatgc agctgaatat tcttaatttt aggtatgaaa aagcgtcggg attgataaaa
     4921 aatctattga attgggtaaa tttaagttga tataataagt tgattagaaa aattaataat
     4981 tcatttaaat tctaatcagc tgtaacgttc cggggcatgt taaactgtct gaatatagaa
     5041 aatacaggtt tctaaggtga ctaagataag ttaaggagga ttccttaaat tctgtgatag
     5101 gaacgtaaac tctctacaag cgtagagcac gtcatcagtc agtctgggtg tctactggct
     5161 gacaacgtca aataagatag gcatgaaaaa ataaggggga acacagaatg tttttaaaac
     5221 accaagatgt gaaacaaaaa aattggagaa tgcgtaaggt caaaaaactc tttgttagtt
     5281 catgtatgct tttaacagtc ggcctcggag ttgccgtacc tactggattc agccaatcta
     5341 atggcgtgat ggttgtaaag gctgatgtta cacaagaagg aagtcctgaa aatgttttga
     5401 agatagctaa ttctttaaag gctacaactg atggtacaaa aataggtaga tatagtgcaa
     5461 taaatactca aaatctagat gtttctgaat ataaatttta ttatgttaac tcaactaaat
     5521 taaataaaaa tcctcaacaa cgcttatggc actatgaaaa accaaaagaa atatatgatt
     5581 ctaccattcg agttgcgggt gatggaactg ctaatattcg tttttatgca gatggagatg
     5641 gtagtaagac tcctaatgga ttctcttttg catataagtt caatactact aatctaaaaa
     5701 cagaccacaa atataagttg tcgttcaagc aaactcaaaa taatgacgaa gaggtttata
     5761 caaagtatct ggtaggtaaa gcaagtgaag gtgtcgctaa ttatttaaca tctgaagaaa
     5821 cttcggtagc tcggaatgtt gagttatttg aaggtaatca aaagacgact gcagagagaa
     5881 aatttgcaaa acatcaatca ggagagaaat ctcttcagtt tatttcaaaa acagatggaa
     5941 ctgctagttt agttttagca gctggtgtaa aaaatactcc caagaaaaat acagatataa
     6001 cctttgacag ttttgaattt attgatataa ccgaaccagc tcaagttagt tctggagaag
     6061 cgattgcagg aaataaaaat cttacggtaa atctaagtgg ttcagacggt agaaaaaact
     6121 tcagcgggga aatcattgaa gtttataaga atggacaatt attaaaaaaa gaaacagtaa
     6181 ctacggcggg taataaagtt aatattgctt tgagtgatga tgttgttttg aaaaaagatg
     6241 atgaaattac ctttaaagta aagcaaggta gttctgggaa aaagaatcaa gcggcaggaa
     6301 aaattattgt tcaacaaaat ccagcagttg tagcggagca agaaaactct aaaaaaagct
     6361 tagaagaagt atataacaaa gctaagaaag ctatttctga tgatgagtgg ttatcggatg
     6421 aggaaaagag agttcagact gcagcagttg aaaaaactta taatgaaggt cagacttcaa
     6481 ttaataatgc agaaacagtg actgaaataa aggatgcttt caaaaaatat tctacgcaag
     6541 gtaataaaat ttctatacct gatcaatata aaaaaggcga aaaagctata aaacaagaac
     6601 aagctcgaaa aagtttgaag caggtacacg aagatacatt aggaaaaatt agatcagatg
     6661 attggttgac tgaagaagaa aaagcaactc aaacagagaa tgcaaaacag agttataatc
     6721 aaggagagaa caagattaat caatcagatt ctctgaagac attaaatcaa atattaaaag
     6781 attatacttc tgaggaaccc aataaaaaag aatcaatacc caacaagtat gaaaaaggga
     6841 agaaagaaga attaaaaaga gacgctgaaa caaaattaag agaagctcaa gaaactacta
     6901 aacaagctat agaaaatgat ccatggttga gtccggaaca gaaaaaagct caaaaagaga
     6961 acgctaaaac aagattagat gcaggtttaa aagctgtcga aactacagaa agcttagata
     7021 aacttaaaga agttgagagt gacttccttg ataaagaaaa agcagaatct attccatctc
     7081 aacatcaagc aggaactcct gaagttgctc gcaaaacttt cttagataac tttgaaaaag
     7141 aagctaaaaa agagattgaa tcaattaaaa atgacgctac attaacagat gctgaaaaag
     7201 caacagctaa agctaaagta gaagcacaac ttcaagaagc taaagataag gctaaggaat
     7261 caaaatcgtt tgatgatttg aagaacattc aagataagtt caattctgaa ttaccacaca
     7321 caacaggtaa gccgcttaaa gatcaacaat ctgatgctat tgcagaatta gagaagaagc
     7381 agcaagaaat tgaaaaagct atctcagaag ataagacatt gtcaaaagat gaaaaagaga
     7441 aacaaattgc tgattctaaa gcgaaattag tcgctgagaa ggaaaaagtt tctaaagcac
     7501 ctgatgctga tgctgttaag aaagctcttg agttaggtaa gcaagaaatt gctaaggctt
     7561 atgttccaca aaacttagaa gatcacaaga aaaaacttct tgcagagttg aagcaaaaag
     7621 caaatgatac tgaaaaagcg attgatttcg ataaaacttt gacagcgaaa gaaaaagaag
     7681 agcaaaaagc gaaaacaaaa gaagaacttc aaaaagcaac agaagctgtt ggagcaattg
     7741 acaatcgtga agaactagat aaaaaagtcc cagaacttaa gaaagctatt caagattctc
     7801 acgttaaagg agaccttgaa ggtgttaaga ataaagctat tgaagatctt caaaaagttc
     7861 atgacgaaac agttgctaaa ataaatggtg atgataccct tgacaaagct actaaagaag
     7921 ctcaagtgaa agaagctgac aaagctttgg cagcaggtaa agatgcgatc actaaagcag
     7981 atgatgctga taaagtgggt gcagctgtta cagagcacac accaaaaatt aaagcagcac
     8041 ataaaactgg tgatcttaaa aaagttcaag aagaggctaa ccaagctctt gacaaagcag
     8101 ctgaaaaaga acgtggagaa atcaataatg atatcacatt aacggcaaaa gacaaagaac
     8161 aacaactgaa agaagttgag acagctctta ctaaagctaa agctaaagtg gcagaagcta
     8221 agacagcaga cgctatcaat gacgcacgtg ataaaggcgt agcaacaatt gatgccgttc
     8281 ataaagcagg tcaagactta ggtgctcgta agtcaggtca agtcgctaaa cttgaagaag
     8341 cagctaaagc aacgaaagac aagatttcag ctgatccaac tttgacaagc aaagaaaaag
     8401 aagagcaatc taaagctgtt aacgctgaac ttaagaaagc gatagaagct gttaacgcag
     8461 ctgacacagc tgacaaggtt gacgatgctc ttggtaaagg tgtcacagac atcaagaacc
     8521 aacataagac aggtgaccct gtcgtagctc gtcgtgaggc tcatggtaag caacttgata
     8581 gagtcgctca agaaactaaa gatgcgattg aaaaagaccc tactttgacg actgaagaaa
     8641 aagctaaaca agttaaagac gtagatgccg ctaaagaaag aggcatggct aagcttactg
     8701 aagctaagaa tgctgatgag ttagacaaag cttacggtga aggtgttaca gacatcaaga
     8761 accaacacaa atcaggtgac cctatcgaag ctcgtcgtgg attacacaac aagtcaatcg
     8821 acgaagtggc gcaagcaact aaggacgcta tcacagcaga tacgactttg actgaagctg
     8881 aaaaagaaac acaacgtggc aatgttgata aagaagcaac taaagctaaa gaagaacttg
     8941 ctaaggctaa agatgctgat gctttagaca aagcgtacgg tgacggtgta accagcatca
     9001 agaaccaaca caagtcaggt aaaggtcttg acgttcgtaa agatgagcac aagaaagctc
     9061 ttgaagctgt tgctaaacgt gtcactgctg aaattgaggc tgatccaacc ttaacaccag
     9121 aagtgagaga acaacaaaaa gcagaggttc aaaaagagct tgaacttgcg actgataaga
     9181 ttgctgaagc taaagatgca gatgaagcag acaaagctta cggtgacggt gtcacagcga
     9241 tcgaaaatgc ccacgttatt ggtaaaggta tcgaagctcg taaagacctt gctaagaaag
     9301 accttgctga agctgctgct aagacaaaag ctctcattat tgaagacaaa acgcttactg
     9361 atgatcaacg taaagaacag ttattaggtg ttgatacaga gtatgctaaa ggtatcgaga
     9421 atattgatgc agctaaggat gctgcaggtg ttgataaagc atatagtgac ggtgttcgtg
     9481 acatcctggc acagtacaaa gaaggtcaaa accttaatga tcgtcgtaat gctgccaaag
     9541 aatttcttct taaagaagca gacaaagtga cgaaactaat caatgatgat ccaaccttga
     9601 ctcatgacca aaaagttgat caaatcaaca aagttgaaca agctaagtta gacgcaatca
     9661 agtctgttga tgatgctcaa acagctgatg ctatcaatga tgctcttggt aagggtattg
     9721 aaaacatcaa caaccaatac caacatggcg atggcgttga tgttcgtaaa gcgactgcca
     9781 aaggcgatct tgaaaaagaa gctgctaaag tgaaagctct tattactaac gacccaacct
     9841 t
//
LOCUS       DAUCLR010000069         9790 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.069, whole genome shotgun sequence.
ACCESSION   DAUCLR010000069 DAUCLR010000000
VERSION     DAUCLR010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9790
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.069"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            9..1268
                     /locus_tag="VGT87_001412"
     CDS             9..1268
                     /locus_tag="VGT87_001412"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEQ3814252.1"
                     /translation="MRKIIINGGKALSGEVAVSGAKNSVVALIPAIILADDIVILDGV
                     PAISDVDSLIEIMELMGATVNYHGDTLEIDPRGVQDIPMPYGKINSLRASYYFYGSLL
                     GRFGQAVVGLPGGCDLGPRPIDLHLKAFEAMGVEVSYEGENMNLSTNGQKIHGAHIYM
                     DTVSVGATINTMVAATKAQGKTVIENAAREPEIIDVATLLNNMGAHIRGAGTDIITIQ
                     GVQKLHGTRHQVIPDRIEAGTYIALAAAIGKGVKITNVLYEHLESFIAKLEEMGVRMT
                     VEEDAIFVEKQESLKAITIKTSPYPGFATDLQQPLTPLLLKADGRGTIIDTIYEKRIN
                     HVPELMRMGADISVIGGQIVYQGPSRLTGAQVKATDLRAGAALVTAGLIAEGKTEITN
                     IEFILRGYASIIAKLTALGADIQLIED"
     gene            1625..2362
                     /locus_tag="VGT87_001413"
     CDS             1625..2362
                     /locus_tag="VGT87_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEQ3814253.1"
                     /translation="MGKEIKVKYFLRRSAFGLVAVSASVLVGSTVSAVDSPIEQPRII
                     PNGGTLTNLLGNAPEKLALRNEERDIDELKKQAIEDKEATTAIEAASSDALEALADQA
                     DTLQSEEAAAVQSDNAASDALEALADQADALQSEEAAVVKADNAASDALEALADQADA
                     LQSEEAEVVQSDNAASDAWEKAATPIALDVKKTKDTKPVVKKEERQNVNTLPTTGEES
                     NPFFTAAALAIMVSTGVLVVSSKCKEN"
     gene            2600..3142
                     /locus_tag="VGT87_001414"
     CDS             2600..3142
                     /locus_tag="VGT87_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEQ3814254.1"
                     /translation="MDIWTKLAVFAFFETPKVILRPFRYEDHWDFYSMVNDTKNLYYV
                     FPEQKTKAASDYLLVHSFIKFPLGQWAIEDKATHQVIGSIRIEHYDAKTRCADIGYFL
                     NYAFWGQGIMTEVVIKLVYLSFHEFGLKTLRIITHLENKASQKVAKKAGFQLKTCFKG
                     SDRNTHKICIYKMYQLTNDR"
     gene            3151..4434
                     /locus_tag="VGT87_001415"
     CDS             3151..4434
                     /locus_tag="VGT87_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEQ3814255.1"
                     /translation="MSKHQDILDYLEKLAIGKKVSVRSISNHLKVSDGTAYRAIKEAE
                     NRGIVETKPRSGTVRIEKKGRVRIDRLTYSEIARISDSEVLAGHAGLGHEFSRFSIGA
                     MTQQNIRRYLVKGGLLIVGDRETIQLLALENHNAILVTGGFPVSKRVIEMANNQRIPV
                     MVTHYDTFTVATMINHALSNIRIKTDLKTVEQVMIPITDYGYLCENSSVEEFNTLIKK
                     TRQVRFPVLDYKRKVIGVVSMRDVVDQLPTTKLTKVMSKNPITARPNTSLANISQKMI
                     FEDLNMLPVTDEENNLLGMITRRQAMENLPNHQPNNPYTYSEQILSNLEETVDYYQVV
                     VEPTMIDSAGNMSNGVISEFLKEISIRALTKKHQKNIIIEQMMVYFLHAIQIEDELKI
                     YPKIITENRRSSTIDIEIFVDDQVIAKAIITTKIN"
     gene            4450..5310
                     /locus_tag="VGT87_001416"
     CDS             4450..5310
                     /locus_tag="VGT87_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEQ3814256.1"
                     /translation="MITLKSAREIEAMDRAGDFLAGIHIGLRDIIKPGVDMWEVEAYV
                     RRRCKEDNVLPLQIGVDGHMMDYPYATCCGLNDEVAHAFPRHYILKEGDLLKVDMVLS
                     EPLDKSIVDVAALDFDNVPEMKKWTGSYTGGLADSCWAYAVGTPSDEIKQLMDVTKEA
                     MYRGIEKAVIGNRIGDIGAAVQEYAESFGYGVVRDLVGHGVGPTMHEEPMVPNYGTAG
                     RGLRLKEGMVLTVEPMINTGTWEIDTDIKTGWAHKTLDGGLSCQYEHQFVITKDGPVI
                     LTSQGEERTY"
     gene            5312..6277
                     /locus_tag="VGT87_001417"
     CDS             5312..6277
                     /locus_tag="VGT87_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEQ3814257.1"
                     /translation="MAEKKWFDKVLSKWQYEPIQVFMRHLQSAEMDLSAIAVAYYLIL
                     TAFPLIVIAANIFPYLNIDIADLLRLMKQNLPKDIFRPASAIVENIFSKPSGSVLGVA
                     TLTGLWTMSRSLTSLQKAINKAYGASQHRDFFIGHLVGLLTSLIILFLLAFALIFSTF
                     SKAAIQVLDKHYHLSDNITTIFLLLIQPITVLIIFVGLMLLYFLLPNVKIKKIRYILP
                     GTLFTSFVMTFLSNLVGNYVVYNVERMVDIKMFGSVMIFIIMLWFIFLARILILGAIF
                     NATYQEMSLGKLEGRSGDMIAILKKTLGNDSDLSPSQSIEDSHTD"
     gene            6388..7671
                     /gene="aroA"
                     /locus_tag="VGT87_001418"
     CDS             6388..7671
                     /gene="aroA"
                     /locus_tag="VGT87_001418"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEQ3814258.1"
                     /translation="MKLRTNAGPLQGTIQVPGDKSISHRAVILGAVAKGETRVKGLLK
                     GEDVLSTIQAFRNLGVRIEEKDDQLVIEGQGFQGLTAPCQTLNMGNSGTSMRLIAGLL
                     AGQPFSVKMIGDESLSKRPMDRIVYPLKQMGVEISGETDRQFPPLQLQGNRNLQPITY
                     TLPISSAQVKSAILLAALQAKGTTQVVEKEITRNHTEEMIQQFGGRLIVDGKRITLVG
                     PQQLTAQEITVPGDISSAAFWLVAGLIIPGSELLLKNVGVNPTRTGILEVVEKMGAQI
                     VYEDMNKKEQVTSIRVVYSHLKGTIISGGLIPRLIDELPIIALLATQAQGTTCIKDAQ
                     ELRVKETDRIQVVTDILNSMGANIKATADGMIIKGPTVLYGANTSTYGDHRIGMMTAI
                     AALLVKQGQVHLDKEEAIMTSYPTFFKDLERLCHD"
     gene            7664..8155
                     /locus_tag="VGT87_001419"
     CDS             7664..8155
                     /locus_tag="VGT87_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEQ3814259.1"
                     /translation="MTKVLLGFMGVGKTTVSKHLSMHCKDMDAIIEAKIGMSIAAFFE
                     QHGEIAFRTIESQVLKDLLFTNDNSVIVTGGGVVVLQENRQLLRKNHQHNILLVASFE
                     TLYQRLKHDKKSQRPLFLKYSKEAFYEFYQQRMVFYEGLSDLVIRVDHRTPEEVANII
                     EGY"
     gene            8363..>9790
                     /locus_tag="VGT87_001420"
     CDS             8363..>9790
                     /locus_tag="VGT87_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEQ3814260.1"
                     /translation="MTKYPMGGLSHHEELRYFYLLRNLSYLSENEKKEFAFLKSKLEI
                     GRAYAPSKQHYRKSKRQEPYFEDDYYNDYSPNDLLEDDDVNHDSSFVPYGLPIYPKED
                     RYLNKKTKLTARRPIDAPQPIDEDDAFLTESVARCALPRSQKRKHKKKGCMKWFFNIL
                     GLLLMTVLMGLGLMFAKGVFDISTNKANYKPAVSQAFDGQETQDGTNILVLGSDQRVT
                     QGSTDARTDTIMVVNVGNHAKKIKMVSFMRDTLINIPGYSYNDNSYDLKLNSAFNLGE
                     QEDHHGAEYVRRALKHNFDIDIKYYVMVDFETFAEAIDTLFPNGVKIDAKFATVGGVA
                     VDSVEVPDDLRMKNGVVPNQTIEVGEQRMDGRTLLNYARFRKDDEGDFGRTVRQQQVM
                     SAVMSQIKDPTKLFTGSAAIGKIYALTSTNVSFPFVVKNGVSVLGSGKNGVEHVTIPE
                     NGDWVDEYDMYGGQALYIDFDKYQKT"
BASE COUNT         3202 a         1586 c         2048 g         2954 t
ORIGIN      
        1 ttgaatgtat gcgtaaaatt attattaatg gcggaaaagc cttatctgga gaagtggcag
       61 tttcaggggc aaaaaacagt gttgttgcgc ttattccagc aatcatctta gccgatgata
      121 ttgtgattct tgatggtgta cctgctatta gtgatgtaga tagtctgatt gaaatcatgg
      181 agcttatggg agcaacagta aactatcatg gtgatacttt agaaattgac cctagagggg
      241 tgcaagacat cccgatgcct tacggaaaga ttaatagtct tcgagcttcc tattatttct
      301 acggtagtct tttaggtcgt tttggacaag cagttgttgg cttacctggt ggttgcgatt
      361 taggaccaag accaattgac ttgcatttga aggccttcga ggctatggga gttgaagtgt
      421 cttatgaggg tgaaaatatg aatttatcga caaatggtca aaaaattcat ggcgctcata
      481 tctatatgga caccgttagt gtcggtgcta caattaatac tatggttgcg gcaacaaaag
      541 ctcaaggtaa aacggttatt gaaaacgctg cacgtgagcc agagattatt gatgtggcta
      601 ctcttttgaa taacatgggt gcgcatattc gtggagcagg tacagatatt attacgatac
      661 aaggtgttca gaaattgcat ggtactagac accaagttat tcctgatcgt attgaagcag
      721 ggacttatat cgctttggcg gcagctattg gaaaaggtgt taagatcact aatgttcttt
      781 atgagcattt agagagtttc attgctaagt tagaagaaat gggtgtccgt atgacggtag
      841 aagaggatgc tatctttgtt gaaaagcaag aatccctcaa ggctattaca attaaaacct
      901 ctccttaccc tggttttgcc actgatttgc aacagccatt aacgcccttg ttgttaaaag
      961 cagatggtcg tggaacgatt attgacacta tatatgaaaa gcgcattaac catgtgccag
     1021 agttgatgcg catgggagca gatatctcag ttattggtgg tcagattgtt tatcaagggc
     1081 cgtctcgcct gacaggagct caggtcaaag ccactgattt acgtgccgga gcggctctag
     1141 tgactgctgg tctaatagct gaaggcaaga cagaaattac taatattgaa tttatcttac
     1201 gtggctatgc tagtatcatt gcaaaattaa cagcactagg ggcagatatt caattaatcg
     1261 aagactaaag agaagctaac gcttctcttt tttatattac atgtgcttag gctaatgata
     1321 cttaggtgca tactttgttg cattttatgg ctttttaact gtcataaaaa agataaaaat
     1381 gatatcacta actaatatta ttaattttaa tgctaaaata atgagttgac ggtagtaaag
     1441 tgataaataa acactcctta aaatattatt ttattaattt tttaaaaaaa taatatataa
     1501 attagataat aaacttgatt ttatgcaatt aatctgtata ataaaagatg gaattattaa
     1561 ttataatttc caaattatcg gtaatttaat atgctaatgc atataaaaat aaaaaggaga
     1621 aacaatggga aaagaaataa aagtgaaata ctttttgcgt agatcagctt ttggattagt
     1681 tgcggtgtca gcatcagtat tagtcggttc aacagtatct gctgttgact cacctatcga
     1741 acagcctcga attattccaa atggcggaac cttaactaat cttcttggca atgctccaga
     1801 aaaactggca ttacgtaatg aagaaagaga cattgatgaa ttaaaaaaac aagctattga
     1861 ggataaagaa gctacgacag ctatagaagc agcaagttca gatgccttag aagcattagc
     1921 ggatcaagca gacactttac aatcagaaga agctgcagca gttcaatcag ataatgctgc
     1981 tagtgacgcc ttagaagcat tggcggatca agcagacgct ttacaatcag aagaagctgc
     2041 ggttgttaaa gcggataacg ctgctagtga cgccttagaa gcattagcgg atcaagcaga
     2101 cgctttacaa tcagaagaag ctgaagtagt tcaatcagat aacgctgcta gtgacgcctg
     2161 ggaaaaagca gcaactccaa tcgctttaga tgttaagaaa actaaagata caaaacctgt
     2221 agttaaaaaa gaagaaagac aaaacgttaa tacccttcct acaactggtg aagagtctaa
     2281 cccattcttt acagctgctg cgcttgcaat aatggtaagt acaggtgtgt tagttgtaag
     2341 ttcaaagtgc aaagaaaatt agttgctatt tgttctagca aatgaaacaa gggaatcgaa
     2401 agattctctt tttctgatat ttaaaaatgc tacagtttat aatagcttga aagactaaat
     2461 gttctcaaac tcattagaac aagctttaat aaaactaacc cagttgtatc aaaggataga
     2521 taaagactaa gactgttata gaatatttgc aaaagatttg ttataatgga cccaatacag
     2581 gtattgtgaa aggtataata tggatatttg gacaaagctt gcagtgtttg ctttttttga
     2641 gactccgaaa gtaatattac ggccttttcg ctatgaagat cattgggatt tttacagcat
     2701 ggttaacgac actaaaaacc tttattatgt ttttccagaa caaaaaacta aggcagcaag
     2761 tgactatctt ttagtacata gttttataaa gtttccttta ggtcagtggg caatagaaga
     2821 taaagcaacc caccaagtaa taggttctat tagaattgag cattatgatg ctaaaacgcg
     2881 ttgtgctgat attggctatt ttttaaacta tgccttttgg ggacaaggaa ttatgacaga
     2941 agtcgtaata aaacttgttt atttatcttt tcacgaattt ggtctcaaaa cgttgcgtat
     3001 tataactcat ttagaaaata aggccagcca gaaagtagct aaaaaagcag gttttcaact
     3061 aaaaacctgt tttaaaggaa gtgatcgtaa tactcataaa atctgcattt ataaaatgta
     3121 ccaactaact aatgataggt gataatcatt atgagcaaac atcaagatat tttagattat
     3181 ttagaaaaac ttgctattgg taaaaaagtg agtgttagaa gcatttcaaa ccacttaaaa
     3241 gtaagcgatg gaacagccta ccgtgctatt aaagaggcag aaaatcgagg tattgtcgaa
     3301 accaaaccaa gaagtggaac tgttcgtatt gaaaaaaaag gacgggttcg tattgaccgt
     3361 ctgacctatt ccgaaattgc tcggatcagt gattcggaag tgctagcggg acatgctggt
     3421 ttaggacatg aatttagtag gttttcaatt ggtgctatga ctcaacaaaa tattcgtcgt
     3481 tacttggtca aaggagggtt attaatagtt ggggatcgtg aaacgattca actcttagct
     3541 cttgaaaacc ataatgccat tttggtgacg ggcggatttc cagtatccaa acgtgttatt
     3601 gaaatggcaa ataaccaacg aatcccagtt atggtaactc attatgatac ctttactgtg
     3661 gcaacaatga ttaatcacgc gctatctaat attcgtatta aaacagatct caaaacagtt
     3721 gaacaggtaa tgatacctat tacagattat ggttaccttt gcgaaaacag ctccgttgaa
     3781 gaattcaata cattaataaa aaaaacacga caagtcaggt ttcctgtatt agattataaa
     3841 agaaaagtga ttggtgtagt tagtatgcgt gatgtggtgg atcagttgcc aacaactaag
     3901 cttaccaagg tcatgtctaa aaatccaata acggctagac caaatactag tcttgctaat
     3961 attagccaga aaatgatctt tgaagattta aacatgttac ctgtcactga cgaagaaaac
     4021 aatttacttg gtatgattac tcgtagacaa gcaatggaaa atttgccaaa tcaccaacca
     4081 aataacccct atacttatag tgagcaaata ctttctaacc ttgaagaaac ggttgattat
     4141 taccaagtag tagttgagcc aacaatgatt gatagtgctg gtaacatgtc aaacggtgtt
     4201 atttcagagt tcttaaagga aattagtatc agagccctca cgaaaaagca ccaaaaaaat
     4261 atcattatcg aacaaatgat ggtatacttt ttacatgcta ttcaaattga ggatgaatta
     4321 aaaatatatc ctaaaatcat tactgaaaat agacgaagca gcaccattga tattgaaatt
     4381 tttgtggatg atcaagtgat tgctaaagct attattacaa cgaaaataaa ttagaggagt
     4441 aacaaaacaa tgataacatt aaaatcagcc cgtgaaatag aagcgatgga tagagcagga
     4501 gacttcttag caggtatcca tattggctta agggatatta ttaagcctgg tgtggatatg
     4561 tgggaagtgg aagcatatgt tcgtcgccgt tgtaaagaag acaacgttct tcctttgcaa
     4621 ataggggttg atggtcacat gatggactat ccttatgcga cctgttgtgg tctaaatgac
     4681 gaagtagcac atgcctttcc ccgtcattac attctgaaag aaggggattt gcttaaagtt
     4741 gacatggtac tcagtgagcc tttggataaa tcaattgttg atgtagcagc tttagatttt
     4801 gataacgtgc ctgagatgaa gaaatggaca ggatcttata ccggtggttt agctgactct
     4861 tgttgggctt atgctgttgg aacaccgtct gacgaaataa agcaattaat ggatgtcacc
     4921 aaagaagcca tgtaccgtgg tattgaaaaa gcagtaattg gcaatcgtat tggtgatatt
     4981 ggcgcagctg ttcaagaata cgctgaaagc ttcggttatg gtgtcgtgcg tgatcttgtt
     5041 ggtcacggtg ttggtcctac aatgcatgaa gagccaatgg taccaaacta tggaacagca
     5101 ggtcgtggac ttcgtctaaa agaaggcatg gtcttgactg ttgagccaat gattaataca
     5161 ggaacttggg aaattgatac cgacatcaag acaggttggg ctcataaaac cctagatggt
     5221 ggtttatctt gccaatacga acaccaattt gtgattacta aagatggtcc cgttatttta
     5281 acaagtcaag gagaagaaag aacttactaa aatggcagaa aaaaaatggt ttgataaggt
     5341 actgtccaag tggcagtatg aacccattca agtctttatg aggcatttac aaagtgcaga
     5401 aatggattta tctgccattg cggttgcgta ttaccttatc ttaacggcct ttccattaat
     5461 tgttattgca gccaatattt ttccttacct caatattgat attgctgatt tgttacgtct
     5521 aatgaagcaa aatttgccta aagatatttt tagaccggct tctgcaattg tagaaaatat
     5581 tttctctaaa ccgtctggaa gtgttttggg agtagcgact ttgacaggat tatggacaat
     5641 gtctcgaagt ttgacatcgc tgcaaaaagc tattaataaa gcctatggcg cttctcagca
     5701 tcgagatttc tttatcggac acttggttgg tttgttgacc agtttaatta ttttattttt
     5761 attagccttt gccttaatct tttcaacatt ttctaaagct gctattcaag tgctggataa
     5821 gcattaccat ttgagtgata atatcacaac gattttctta ctcctcattc aacccattac
     5881 agttttgatt atttttgttg gcttgatgct actatatttt ttattaccca atgttaagat
     5941 taaaaaaatt cgctacattt taccaggaac cctatttaca tcttttgtga tgacattttt
     6001 gagtaatctg gttggcaatt acgttgtgta taatgttgag cggatggtag atattaaaat
     6061 gtttggttca gttatgattt ttatcatcat gctatggttt attttcttag caagaatcct
     6121 gattttagga gctatattta atgcgaccta tcaagaaatg tccttgggga aattagaagg
     6181 tcgtagtggt gatatgatcg ctatccttaa aaaaacactt gggaatgatt cagatctatc
     6241 tccttcacag tctatcgaag atagtcacac tgattgatac aaaaaagata ataagtcgtg
     6301 gagtgagaaa ctattttctt gcttcatttt ttatgaggat gaatagaaaa tcattgatct
     6361 tagcaaatag aaaggcagat gaaacggatg aaattgcgaa caaatgcagg accgttacag
     6421 ggaacgattc aagttccagg agacaagtcc attagtcacc gggcagtcat attaggagcc
     6481 gttgctaaag gggaaacacg agtaaaagga ctcttaaaag gagaagatgt gctttccacg
     6541 attcaagcct ttaggaattt gggggtccgt atcgaagaaa aagatgacca gcttgtgata
     6601 gaaggtcaag gttttcaagg attgaccgct ccttgtcaga cacttaatat gggaaattct
     6661 ggaacctcta tgcgcctcat tgctggttta ttggcaggtc aaccttttag tgtcaaaatg
     6721 attggcgatg aaagcttatc caaacgtcct atggatcgga ttgtttatcc gttaaagcaa
     6781 atgggggttg agatatctgg agaaacagat aggcaatttc ctccactaca attgcaaggt
     6841 aatcgtaatt tacaaccaat cacatatact ttgcctatct cttctgctca ggttaagtca
     6901 gctattttgt tagcagcttt gcaagcaaaa ggaacaacac aggttgttga aaaagagatt
     6961 actcgaaacc atacagaaga aatgattcaa caatttggtg gacgattgat agttgatggt
     7021 aaacgcatta ctttggtggg accccagcag ctcactgctc aagagataac tgtccccgga
     7081 gatatttcta gtgcagcttt ttggcttgtc gccggcttaa ttattcctgg ttcggagctc
     7141 cttttaaaga atgttggagt caatccgaca cgaacaggga ttcttgaagt ggttgagaaa
     7201 atgggagcac agattgtcta tgaagatatg aataagaaag agcaagtaac tagtattcga
     7261 gtggtatatt ctcacttgaa aggaactatc atatcaggag gtcttatccc tcggttgatt
     7321 gatgaacttc ccatcattgc acttcttgca acccaagcac aaggaacaac ctgtattaaa
     7381 gatgctcaag aattacgagt caaagaaaca gatcgtattc aagtagtaac tgatattctt
     7441 aacagtatgg gagctaatat caaagcgaca gcagatggca tgattatcaa aggccctacg
     7501 gtactatatg gggctaacac aagtacctat ggagatcatc gtattggtat gatgacagca
     7561 attgcagcct tgcttgttaa acaaggacag gtccatttgg ataaagaaga agccatcatg
     7621 acgtcttatc cgacgttttt caaagattta gaaaggctgt gtcatgacta aagtgttatt
     7681 gggatttatg ggagtgggaa aaacaacggt ttcgaaacac ctaagtatgc actgtaaaga
     7741 tatggatgct attattgaag ctaaaatcgg tatgtctatt gcagcttttt ttgaacagca
     7801 tggagaaata gcatttcgaa caattgaaag tcaagtgtta aaagatctat tgtttacaaa
     7861 tgataatagt gtcatcgtaa caggtggcgg tgtagtggtt ttacaagaaa atcgacaatt
     7921 actgagaaaa aatcaccagc ataatatttt attggtagct tcttttgaaa ctttgtacca
     7981 gcgtttaaaa catgataaaa agtctcaaag acctctattt cttaagtact ctaaggaagc
     8041 attttatgaa ttttatcaac aacgtatggt attttatgag ggactttcag atttagtaat
     8101 cagagttgac catcgtacgc ctgaagaagt tgccaacatc attgagggtt attagcaggt
     8161 cattgtcgta atctgatatt tatggtttta aacaggtatc tgtctaaagt acttcttatg
     8221 gcaagttggc tttattaaat attaggaaat aatcttgatt attctataat gattaaaatt
     8281 ttgagaggac aatctggcta atataggtta taatggtagt attcgttaaa agtgcaattg
     8341 taatactaag agatgataag atatgacaaa ataccccatg ggtggcctta gtcaccatga
     8401 agaattacgt tatttttatc tcctaaggaa tttgagttac cttagtgaga atgaaaaaaa
     8461 agagtttgct tttttaaaat ctaaattgga aattggtcga gcttatgcgc cttctaagca
     8521 gcattatagg aagtcaaaac ggcaagaacc ttactttgaa gatgactact ataacgacta
     8581 ctcaccaaat gatttactag aagatgatga cgtcaatcat gactcttctt ttgttccata
     8641 tggtttaccg atatatccta aagaagacag atatttaaac aaaaaaacaa aacttacagc
     8701 tcgtagacct atagatgctc cgcagcctat tgatgaagac gatgcttttt taacagaatc
     8761 tgttgctcgt tgtgctcttc ctagatctca aaaaagaaag cataagaaaa aaggatgtat
     8821 gaaatggttt tttaacattc ttggcttatt gctaatgact gtgcttatgg ggttagggtt
     8881 gatgtttgca aaaggggttt ttgatatttc tactaataaa gctaattaca aaccagctgt
     8941 ttcccaagct tttgatggtc aagaaactca agatggtacg aacattctgg ttttgggttc
     9001 tgaccaacga gttactcaag gttctacaga tgctcgcaca gataccatta tggttgtcaa
     9061 tgtcggcaac catgctaaaa aaataaaaat ggtctctttt atgcgtgaca ctttgattaa
     9121 cattccagga tacagttata atgataattc gtatgatctt aaattaaata gtgcatttaa
     9181 tcttggagag caagaagatc accacggagc agagtacgtt cgacgggcct taaaacataa
     9241 ttttgatatc gacattaagt attatgtgat ggtagatttt gaaacatttg cagaagctat
     9301 tgacaccttg tttccaaatg gtgttaagat tgatgccaaa tttgcgacag taggtggtgt
     9361 agctgttgac tccgtagaag ttccagatga cctgcgaatg aaaaatgggg ttgttcccaa
     9421 ccaaaccatc gaagttggag aacaacgcat ggatggtaga accttgctca actatgctcg
     9481 tttccgtaaa gatgacgaag gagatttcgg acgaaccgtc cggcagcaac aggttatgtc
     9541 agctgttatg tcccaaatca aggacccaac taaactcttt acagggtctg cggctattgg
     9601 taaaatctat gcgctgactt cgaccaatgt ttcttttcca tttgttgtta aaaatggtgt
     9661 ttctgtttta ggaagtggga aaaatggagt tgagcacgtg accatcccag aaaatgggga
     9721 ttgggttgac gaatacgata tgtatggagg acaagctctt tacatagatt ttgataagta
     9781 tcagaaaacg
//
LOCUS       DAUCLR010000070         9561 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.070, whole genome shotgun sequence.
ACCESSION   DAUCLR010000070 DAUCLR010000000
VERSION     DAUCLR010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9561
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.070"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..458
                     /locus_tag="VGT87_001421"
     CDS             <1..458
                     /locus_tag="VGT87_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEQ3814261.1"
                     /translation="KTGNISLTDIEQAIPKSLQDNIFDLTRLVLRGKIDAARDLIHDL
                     RLSGEDDIKLIAIMLGQFRLFLQLTILARDVKNEQQLVISLSDILGRRVNPYQVKYAL
                     KDSRTLSLAFLTGAVKTLIETDYQIKTGLYEKSYLVDIALLKIMTHSQK"
     gene            555..1160
                     /locus_tag="VGT87_001422"
     CDS             555..1160
                     /locus_tag="VGT87_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEQ3814262.1"
                     /translation="MAIILPELPYAYDALEPQFDAETMTLHHDKHHATYVANTNAALE
                     KHPEIGENLEELLADVTKIPEDIRQALINNGGGHLNHALFWELLSPEKQDVTPDVAQA
                     IDDAFGSFDAFKEQFTAAATGRFGSGWAWLVVNKEGQLEITSTANQDTPISEGKKPIL
                     ALDVWEHAYYLNYRNVRPNYIKAFFEIINWKKVSELYQAAK"
     gene            1327..1550
                     /locus_tag="VGT87_001423"
                     /pseudo
     CDS             1327..1550
                     /locus_tag="VGT87_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922433.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1538..1765
                     /locus_tag="VGT87_001424"
     CDS             1538..1765
                     /locus_tag="VGT87_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814263.1"
                     /translation="MDALGLYYDDNHLSLVNTVKVYLKEFGIGESQIAFSYKDLNSGR
                     TAAMNDHQPMIAGSTYKLPLNMLVVDKANYP"
     gene            1762..2339
                     /locus_tag="VGT87_001425"
                     /pseudo
     CDS             1762..2339
                     /locus_tag="VGT87_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S11"
     gene            complement(2453..3673)
                     /locus_tag="VGT87_001426"
     CDS             complement(2453..3673)
                     /locus_tag="VGT87_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HEQ3814264.1"
                     /translation="MKLLGKWLFFLMFLTFCVSFWQKDYQRIAFEKEHKLYQDLVAPF
                     YQNYPKLRELQLSEAIKLRHDLLDYVRKLDKDGWSYKAIQKGYLEQLAVRGNSYHFEQ
                     LYSRIRLIGSPDFQKLWQQEEMAQTPQEAQKRLQLLLTYLKMPDELTGQSKQTQQVLA
                     QLSPKLSPTDPFWDQLSTLIQAYYVNLEHIPYSRFNRKIHQLRYLISTQQMEWVRSHY
                     GNKGKTDADALAKYLATLEDDDYALYESSRYHNKVASILDANGNHQAVYTDNIPQANY
                     KILIHFHSEFILSESGQFLVALDPDNLTRNSIVNGSSFNYGNQNDDLHRLLDIDPILL
                     FDPAFIEKAINSPDATFLVPDLEQQRDKHNPIYSRNGKSSKQLTRAAVKKFKKLLHHY
                     QETRGDNKTSKTQH"
     gene            complement(3680..4708)
                     /gene="queA"
                     /locus_tag="VGT87_001427"
     CDS             complement(3680..4708)
                     /gene="queA"
                     /locus_tag="VGT87_001427"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEQ3814265.1"
                     /translation="MNTNDFDFELPEELIAQTPLEKRDSSKLLIIDHRQKTMVDSHFD
                     HIIDQLNPGDALVMNNTRVLPARLYGEKPDTHGHVELLLLKNTQGDQWEVLAKPAKRL
                     KVGSQVNFGDGRLKATIIDELEHGGRIVEFSYDGIFLEVLESLGEMPLPPYIHEKLED
                     AERYQTVYAKENGSAAAPTAGLHFTTDLLKKIEAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHDMHSEFYSLSEEAAQTLRDVKQAGGRVVAVGTTSIRTLETIGSKFQGDIQADSG
                     WTNIFIKPGYQFKVVDAFSTNFHLPKSTLVMLVSAFAGRDFVLEAYRHAVDEKYRFFS
                     FGDAMFVN"
     gene            4910..5614
                     /gene="nagB"
                     /locus_tag="VGT87_001428"
     CDS             4910..5614
                     /gene="nagB"
                     /locus_tag="VGT87_001428"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEQ3814266.1"
                     /translation="MKIIRVQDQIEGGKIAFTLLKDSLAKGAKTLGLATGSSPISFYQ
                     EMVKSPLDFSDLTSINLDEYVGLSVESDQSYDYFMRQNLFNAKPFKKNYLPNGLATDI
                     EAEAKRYDQIIAEHPIDFQVLGIGRNGHIGFNEPGTSFEEETHVVDLQESTIEANSRF
                     FTSIEDVPKQAISMGIASIMKSKMIVLLAFGQEKADAIKGMVFGPITEDLPASILQKH
                     DHVIVIVDEAAASQLD"
     gene            5733..6449
                     /locus_tag="VGT87_001429"
     CDS             5733..6449
                     /locus_tag="VGT87_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEQ3814267.1"
                     /translation="MRLDKLLEGTKVGSRSQVKKLIKAQGVWVDHMPARNGRQNVDPG
                     LQLIEVTGQRVTHPKHSYIILNKPSGVVSAKKDTNYLTVIDQLAEEDKSPDLYPVGRL
                     DRDTEGLVLLTDNGPLGFRMLHPSHHVSKTYLVTVNGLLAEDASDFFAAGICFPTGEQ
                     CQPAQLTILKADTDQSQASLTISEGKFHQVKKMFLTYGLKVTSLKRTHFAGLELGHLA
                     SGEYRYLTASERQLIKTYLD"
     gene            6746..7708
                     /locus_tag="VGT87_001430"
     CDS             6746..7708
                     /locus_tag="VGT87_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEQ3814268.1"
                     /translation="MTKILTALDGKNQLISLVTQPISTKPPFRCPACKSPVRLRQGTI
                     RRPHFAHVQLAHCQFQAENESEEHLTLKAKLYTSLVRTEAVCIEKYLPELQQIADLWV
                     NDKLALEIQCSPLPVERLKKRTKAYQEKGYPVRWLLGRKLWLNTHLTALQKQFLYFSS
                     SLGFHLWELDAAANLLRLKYLIHEDLFGKVSYLTKTISLDHNIMEMFRLPYQQEILYS
                     YQKKMTVNLSKRIQRALLARHPKWLRRQEKAYLSGYNLLMLTTDAFYPQWRPVQSSSG
                     FCQIKGNLRPYYESFKVYYKKEKDKKVQTLFSPKYYVKMDSNRK"
     gene            7721..9526
                     /gene="pepF"
                     /locus_tag="VGT87_001431"
     CDS             7721..9526
                     /gene="pepF"
                     /locus_tag="VGT87_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEQ3814269.1"
                     /translation="MTDNRSHLEEKYTWDLSTIFATDKDWEAEVSDLATEVEASKGFA
                     GHLLDSSANLLKVTKTYLELARRVEKVYVYAHMKNDQDTTVAKYQEYQAKASGLYAKF
                     SEVFSFYDPEVMALSQEDYQAFLAETPDLKRYHHFFDKLFQTRDHVLSQAEEELLAGA
                     QEIFNGAEETFSILDNADIVFPVVKNDKGEDVELTHGNFISLMESKDRSIRQAAYEAM
                     YSTYEQFQHTYAKTLQTNVKVQNYKARVHKYDSARQAAMAANFIPEAVYDTLLETVNK
                     HLPLLHRYLKLRQEVLGLDDLKMYDVYTPLSETDLAIGYDEALEKAEKVLAVFGKDYA
                     DRVHRAFTERWIDVHVNKGKRSGAYSGGSYDTNAFMLLNWQDTLDNLYTLVHETGHSL
                     HSTFTRETQPYVYGDYSIFLAEIASTTNENIMTEALLNEVQDEKERFAILNHYLDGFR
                     GTVFRQTQFAEFEHAIHQADQKGEVLTSEYLNQLYADLNEKYYGLSKKDNYFIQYEWA
                     RIPHFYYNYYVYQYATGFVAASYLADKIVHGTQDDIDHYLAYLKSGNSDYPLEVIAKA
                     GVDMAKGDYLEAAFKVFDERLTELEVLVSKGIHKS"
BASE COUNT         3001 a         1721 c         1946 g         2893 t
ORIGIN      
        1 aaaaaacggg aaatattagc ctaactgata ttgagcaagc cattcctaaa agtttacaag
       61 acaatatttt tgacttaacc agactcgttc tacgaggaaa aatcgatgca gctagagatt
      121 tgattcatga tttacgctta tcaggggaag atgatattaa attaattgct attatgttgg
      181 gtcaatttcg cttatttttg cagctgacta ttcttgctag agacgtaaaa aacgagcaac
      241 aactagtgat tagtttatca gatattcttg ggcggcgggt taatccttac caggtcaagt
      301 atgcgttaaa ggattctagg accttatctc ttgcctttct aacaggagcg gtgaaaacct
      361 tgattgagac agattaccag ataaaaacag gactttatga gaagagttat ctagttgata
      421 ttgctctctt aaaaatcatg actcactctc aaaaataggc attggaccgc tttccaagag
      481 ataatcaaat tagttgaaag ctcttatttt ttgcgataag atagacgtat cataaaggaa
      541 aagaggacag gaaaatggct attattttac cagaacttcc atacgcgtat gatgctcttg
      601 aaccacagtt tgatgcggag acaatgactc ttcatcatga taaacaccat gccacttatg
      661 ttgctaatac caatgcggct ttagaaaaac acccagagat cggagaaaat ctggaggaac
      721 tattggcaga tgtcactaag attccagaag atattcgtca agccttaatt aataatggtg
      781 gcggacacct aaaccatgcg cttttctggg aactattatc acctgaaaaa caagacgtca
      841 ctcctgatgt tgcacaagcc attgatgatg cttttgggtc atttgacgcc tttaaagaac
      901 aatttacagc agctgctact ggacgttttg gttcaggttg ggcatggtta gttgttaata
      961 aagaaggaca actcgaaatc acatcaaccg ctaaccaaga tactccaatt tcagaaggca
     1021 aaaaaccaat tctagcgctt gatgtgtggg agcatgctta ctacttgaat tatcgtaatg
     1081 tacgtccaaa ttatatcaaa gctttctttg aaatcattaa ttggaaaaaa gtatcagaac
     1141 tttaccaagc cgctaaataa gaaaagggtt tccaatctca aaaagagatt ggttttttaa
     1201 gtgcttttgt gaataacagt gtggggacat cattttttag aaaagataaa aatagtttgt
     1261 ctttttaatg taattccttg atttttagct aaatttagct agaatagaat ggataggaga
     1321 ttttaggtgt taagaaagaa gaaaactacc attgtaggga ttattttagg aatgatgata
     1381 gtgaccttac ttgtcggctg tttgactggt aaaaagaaag gctttcctcc taaagcaggt
     1441 gagcggcagc aaacagctga cttaaaaatg aaaaaagatt agaaaaactg ttcaaaaacc
     1501 gttacctgaa aaatctaaag aggctaagca agctgttatg gatgccttag ggctctatta
     1561 cgatgacaac catttgagtt tggtcaatac ggtcaaggtg tatttaaagg aatttggcat
     1621 tggtgaatct caaattgcct tttcctacaa agacctcaat tcaggcagga ctgctgccat
     1681 gaatgatcat cagccaatga ttgcaggaag cacctataag ctgccattaa acatgctggt
     1741 ggtagataag gcaaattatc catgacagag cgttttgaca ttacagaaac tgattatgaa
     1801 tatgatcagg aacatcatgc ctatgttgct caattcaatg gtgccatgtc tattccggat
     1861 atgcaagata ctcagaaatc acgccagctt atgctctagc agaacgattg ggtggcatga
     1921 ataaggctta ccaattattt gaccgctacg ggaaggtatc cggggctatt acgaccattg
     1981 atcgtaatgg caataaaatc accaccgctt actatttgca agttttggat tacttgtggc
     2041 agcatcagga taaatataag gatatcttgt actacattgg tgaatctttc cctgatttgt
     2101 attacaagac ttatttgtct catgtaaagg tttatcaaaa accgggttat gtcagagaag
     2161 ccttaaatgt aggtgccatt gtttgcgaag agtctcctta tttgattgct ctttattcat
     2221 ctggtttggg tggagcaaca caagcatcag aggaagtcaa tggccttggt tatgtgcagc
     2281 ttgttcagct aacatatgta attaatgagt ggcatagagt caatcacaac atgatctaat
     2341 actcagttga cattagtctg atagtagaca tacttactga tgacagtcac taaaataagt
     2401 tgttgctcat taaaaaagct tggcctgttt cttcaggtca agctttttga gattaatgct
     2461 gtgtttttga ggtcttattg tcacctcttg tttcttgata atggtgaagc aattttttga
     2521 attttttaac ggcagcgcgt gttagctgct tagatgattt gccatttctg ctgtaaatgg
     2581 gattgtgctt atccctttgc tgttctaaat cgggaactag aaaagttgca tcaggactat
     2641 taattgcttt ttcaataaaa gccggatcaa aaagcagaat agggtcaata tcaagcagac
     2701 ggtgcaagtc atcgttttgg ttgccataat tgaaagaaga accattgaca atactatttc
     2761 gcgttaggtt atcaggatct agtgctacta aaaattgtcc agattctgac aaaataaact
     2821 ctgagtgaaa atgaatcaaa atcttatagt tggcttgagg aatattgtct gtatagactg
     2881 cctgatgatt gccattagca tctaaaatac tagcaacctt attatggtat ctagaagatt
     2941 cataaagagc ataatcgtca tcttctagag tagccaaata tttggctaag gcatccgcat
     3001 cggttttccc tttattccca taatgtgagc gaacccactc catttgctgg gttgatatga
     3061 ggtaacggag ttgatgaatt ttccgattaa aacggctata aggaatgtgt tcgagattaa
     3121 cataataagc ctgaatcagg gtagataatt ggtcccaaaa aggatctgtt ggcgatagct
     3181 ttggacttaa ttgtgccaat acttgctgtg tttgttttga ttgtcctgtc aactcgtctg
     3241 gcatctttaa atacgtcaag agcagttgta accgtttttg agcttcttga ggcgtctgtg
     3301 ccatctcttc ttgttgccaa agcttttgaa agtcaggtga tcctatcagg cgaatgcgac
     3361 tataaagttg ttcgaaatga taagagtttc ctctaacagc gagttgttca aggtatcctt
     3421 tttggatggc tttataggac catccgtcct tatcgagctt tcgaacataa tcaagcaagt
     3481 cgtggcgtaa tttaatagct tctgaaagct ggagttctcg taatttagga tagttttggt
     3541 aaaaaggtgc cactaaatct tgataaagtt tgtgttcttt ttcaaaggca atccgctgat
     3601 agtctttctg ccaaaaactg acgcaaaaag tcagaaacat caagaagaat agccattttc
     3661 caagtaattt cataggtttt tagttgacaa acatggcatc gccaaagcta aagaaacgat
     3721 acttttcgtc aaccgcgtgt ctgtaagctt caaggacaaa gtcacgccct gcaaaggctg
     3781 atactagcat cacaagggtt gattttggaa gatggaaatt agttgaaaaa gcatcaacta
     3841 ccttgaattg gtagcctggt ttaataaaaa tattggtcca acctgaatca gcttgaatgt
     3901 ccccttggaa cttactcccg atggtttcca acgtacgaat agaggtggtt cctacagcta
     3961 caacacgacc gccagcttgt ttgacgtctc ttagggtttg cgctgcttct tctgagaggg
     4021 aataaaattc cgaatgcata tcatgctcat cgaggttatc cacggaaaca ggtctaaaag
     4081 tcccaagccc tacatgtagg gtcaaataaa ccaaatgtac ccctttggct tcgatttttt
     4141 tgagcaagtc tgttgtgaag tggagaccag cagttggggc ggccgcagag ccattttctt
     4201 tagcataaac ggtttggtat cgttcagcat cctctaattt ttcgtgaata taaggaggca
     4261 atggcatttc gccgaggctc tctaaaactt ctaagaaaat cccatcatag ctgaattcaa
     4321 caattcgtcc accatgttct aattcgtcga taatggtggc tttgagacgc ccatctccaa
     4381 aattgacttg gctaccaacc ttgagccgtt tggctggctt ggctaaaact tcccattgat
     4441 ctccttgtgt atttttaagc aaaagcaatt ccacatgacc atgagtatcg ggtttttctc
     4501 cataaagtct ggcaggtaag acacgggtat tattcatgac gagggcatcc cctggattta
     4561 attgatcaat gatatggtca aaatggctat caaccatagt tttttgacgg tgatcaataa
     4621 tcaatagttt tgaactgtct cttttttcga gtggggtttg ggcaatcaat tcttctggta
     4681 actcaaaatc aaaatcgtta gtattcattg ggactccttt aaagccacta gtaaatctct
     4741 tcctagtaac tctttcatgt aagataaatc ttttctatta tatcacttgt cgtctaaccc
     4801 gtcatcttta atctttcatc agaaagtaaa ggataggctt gacaaaaatt ggtatatacc
     4861 atataataaa aacacgaaag gtctatacca attagaaaag gagaatgaca tgaaaattat
     4921 tcgggtccaa gatcagattg aaggtgggaa aatcgctttt accttattga aagatagctt
     4981 agctaaggga gctaagacgt taggattagc aacaggaagt tcaccaatta gtttttacca
     5041 agaaatggtc aaaagtcctt tggatttttc ggacttaaca agtattaact tggatgaata
     5101 tgtcggtctt tcagtagaga gcgatcagag ttatgattat tttatgcgtc aaaacctttt
     5161 taatgcgaag ccttttaaaa agaattactt gcccaatggt ttagcaactg atatcgaagc
     5221 tgaagctaaa cgatatgacc aaatcattgc agagcatccg attgattttc aagtgcttgg
     5281 gattgggcgt aatgggcaca ttggttttaa cgaacctggg acctcttttg aagaagaaac
     5341 gcatgtggtg gatcttcaag aatcaaccat tgaagctaat agtcgtttct ttacaagtat
     5401 tgaggatgtg ccaaaacaag ctatctctat gggaattgcc tccatcatga agtccaaaat
     5461 gattgttctt cttgcctttg gacaagaaaa agctgatgcg atcaagggaa tggtttttgg
     5521 accaatcaca gaagacctcc cagcaagtat tctccaaaaa catgatcatg ttatcgtcat
     5581 tgtggatgaa gctgctgcgt cacaattaga ctaacctaac cagtcgttaa accttgtaag
     5641 aacaaaaatg aggagatagt caacctcatt taagaagaag tcttgtaact tctatttctt
     5701 ttgcaagggt aaatttatgt tacactacta atatgcgttt agataaatta ttagaaggta
     5761 caaaagttgg ctctagaagt caagtgaaga aactgattaa ggctcaaggg gtttgggttg
     5821 atcacatgcc tgccagaaat ggtcgacaaa atgttgatcc tggcttgcaa ctgattgagg
     5881 tgactggaca gcgagtgacg catcctaaac attcttacat tatcctcaac aagccatcag
     5941 gggtggtctc agctaagaaa gatactaact atttaaccgt tattgatcag cttgctgaag
     6001 aagataaaag tccagacttg tatcctgttg gcagattgga tagagatacc gaagggttgg
     6061 ttttactcac tgataatggt cccctagggt ttcggatgtt gcaccctagt catcatgttt
     6121 ctaaaacgta tcttgtaacc gttaatggct tgttagcaga agatgcctca gatttttttg
     6181 cagctggtat ttgtttccca acaggtgaac aatgccaacc agctcaattg actattctta
     6241 aagccgatac agaccagtca caggctagtt tgaccatttc agaaggaaaa tttcaccaag
     6301 tgaaaaaaat gtttttgacc tatggtttga aagtgacctc tctgaaacgg acgcactttg
     6361 caggactgga actcggtcat ttagccagcg gagaatatcg ttatctaaca gcgtcagaaa
     6421 ggcaactcat taaaacgtat ctggactgag caaaacatgc tttcaaaaag aagctattct
     6481 tgcttaatca ataaagtgtc cattgactat tatagtcatg atgagtccga cttttgctat
     6541 gtttagtcat cttgttgtta caaaatcaga taagcaaaaa gattcaattc ccactatctc
     6601 cttaacctga agcgttataa aaacccttgt gttaaactct cataaaattg gaaatgggag
     6661 tgttttttga tggctaaaaa aaactaaagc tacttaaagt ttggtactta gttttcgaat
     6721 aataaggtgg aagatctaat aggaggtgac aaaaatatta accgctttag atggtaagaa
     6781 tcagctcatt tcgctagtaa ctcagcctat ttcaacgaaa ccgccttttc gttgcccagc
     6841 ttgcaaatct cctgttcgct tgcgtcaggg gacaattaga cgacctcatt ttgcccatgt
     6901 acaactagct cattgtcaat tccaagcaga aaatgaatct gaggaacatt tgacgttgaa
     6961 ggcaaaactt tacaccagtt tagtccgaac agaagctgtc tgtattgaaa aatatcttcc
     7021 tgagctgcaa cagatagcag acctttgggt gaatgataag ttagccttag aaattcagtg
     7081 cagtcccttg ccagttgagc gtctaaagaa acgcaccaaa gcctatcaag aaaaggggta
     7141 tccggtacga tggttactgg gaagaaaatt gtggcttaac actcacttaa ctgctttgca
     7201 aaaacagttt ctttactttt catctagttt gggctttcat ttgtgggagc tggatgcagc
     7261 tgcaaatctg ctacgtctca aatatttaat ccatgaagac ttatttggta aggtaagtta
     7321 cctaaccaaa accatttctt tagaccacaa tattatggaa atgtttcgat taccctatca
     7381 gcaagaaatc ctttattctt atcagaaaaa aatgactgtg aacctatcta aaagaataca
     7441 aagagctctt ttagctagac acccgaaatg gctacgacga caagaaaagg cttacttatc
     7501 aggctataat ttacttatgt taacaactga tgctttttat cctcaatgga gaccagttca
     7561 atcttccagc ggtttttgtc aaattaaagg aaatcttcgc ccttattatg aaagttttaa
     7621 agtctattat aaaaaagaaa aggataagaa ggtgcaaacg cttttttcac caaaatatta
     7681 tgttaaaatg gatagtaata gaaaatgaga aggagatgtt atgactgata atcgcagcca
     7741 tttagaagaa aaatacactt gggatttgag cactattttt gcaacagata aggattggga
     7801 agctgaagtc agtgacttag ctactgaagt tgaggcatct aaaggttttg ctggtcattt
     7861 gctggattct agtgctaatt tgttaaaggt tacaaaaaca tatttagaat tggctcgtcg
     7921 tgttgagaaa gtctatgtct atgctcatat gaaaaatgat caagacacga ctgttgctaa
     7981 atatcaagaa tatcaggcta aggcttctgg cttgtatgct aaatttagcg aagtgttttc
     8041 attctatgac ccagaagtta tggcattgag tcaggaagat taccaagctt ttcttgcgga
     8101 aacacctgat ttaaaaaggt atcatcattt ctttgacaaa ttgttccaga cacgtgacca
     8161 tgtgctcagc caggctgaag aggaattatt agcaggagcc caagaaattt tcaacggcgc
     8221 tgaagaaacc tttagtattc ttgataacgc tgatattgtc tttccagtag ttaaaaatga
     8281 taagggggaa gatgttgaat taacccatgg taacttcatt agcctaatgg aatctaagga
     8341 ccgtagcatt cgccaagctg cttatgaagc catgtatagt acttatgaac agttccaaca
     8401 tacttacgct aaaaccttgc aaaccaatgt gaaagtgcaa aactataagg cgcgtgttca
     8461 taaatacgat tcagcacgtc aagctgccat ggctgctaac tttattccag aagctgttta
     8521 cgacacttta ttagaaacgg ttaacaaaca tttaccactc ttacaccgtt atttgaaatt
     8581 gcgtcaagag gttcttggtt tagacgattt gaagatgtat gatgtttaca caccattatc
     8641 tgaaacagat ttagccattg gttatgatga ggctttggaa aaggctgaga aggttttggc
     8701 tgtttttggt aaagattacg cagatcgtgt gcatcgtgct tttaccgaac gttggattga
     8761 tgttcatgtc aataaaggga agcgttctgg agcttattcg ggtggttcct atgataccaa
     8821 tgcctttatg ctattaaact ggcaagatac cttagacaat ctatacactt tagttcatga
     8881 aactgggcac agtttgcatt ctactttcac tcgtgaaaca cagccttatg tgtatggaga
     8941 ttacagtatt ttcttggcag aaattgcatc cacaacgaat gaaaatatta tgacagaagc
     9001 tctcttaaat gaggttcaag atgagaaaga acgctttgcc attttaaacc attacctcga
     9061 tggtttccgt ggaacagttt tccgtcaaac ccaatttgct gagtttgaac atgccattca
     9121 tcaagctgat caaaaaggag aagtgttaac aagtgaatac cttaaccagc tatacgctga
     9181 tttaaatgaa aaatattatg gtttaagtaa aaaagataat tattttattc agtatgaatg
     9241 ggcacgtatt cctcatttct attacaatta ttatgtttac caatatgcaa caggttttgt
     9301 agctgcaagc tatttagcgg ataagattgt gcatggcaca caagacgata tcgatcatta
     9361 cttggcctac ctcaagtcag gaaattctga ttacccactt gaagtgattg ctaaagcagg
     9421 cgttgatatg gcaaaaggtg attatttgga agccgccttt aaagtctttg acgaacgtct
     9481 aacagaactt gaagtactcg tgtccaaagg catacacaaa agctaaaatc acaaaagtca
     9541 aggacaaaaa gagtttagga t
//
LOCUS       DAUCLR010000071         9541 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.071, whole genome shotgun sequence.
ACCESSION   DAUCLR010000071 DAUCLR010000000
VERSION     DAUCLR010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9541
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.071"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..1954)
                     /gene="recJ"
                     /locus_tag="VGT87_001432"
     CDS             complement(<1..1954)
                     /gene="recJ"
                     /locus_tag="VGT87_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEQ3814270.1"
                     /translation="MIKSKYSWKIKDKKPDDGFFKLAKTKGLTQTAAQLIYDRGIRTE
                     EALDEFLTADLSQLHDPYLLHDMTKAVPRIRQAIEEGERILIYGDYDADGMTSASIVK
                     ETLDMMGAEPLVYLPNRFTDGYGPNQSVYKYFIEQEAVSLIITVDNGVAGHEAIRYAQ
                     EQEVDVIVTDHHSLPEELPEAFAIIHPEHPDADYPFKHLAGCGVAFKLATALLESLPT
                     DCLDLVAIGTIADMVSLTGENRVLVKNGLAMLKHTERVGLQELMSLSPIDLEHFNEDA
                     IGFQIAPQLNALGRLDDPNPAIELLTGFDDQEAQAIALMIKKKNEERKALVQDIFDQA
                     MAMVDPQKPVQVLAQAGWHPGVLGIVAGRIMETIGQTVVVLTIDNGFAKGSARSLEAI
                     NIFEALNGKRELFTAFGGHAGAAGMTLPVDNLEALSDFLCQFVIERGLDQTAKNTLTI
                     DESLSLDDLSLDILKSLDKLAPYGMDHQKPVFYVKDIRVSQARTIGQDQSHLKFKVSQ
                     GKASFDVLAFGQGSQLQEFRQATGLELAVTLSVNHWNGNTSLQFMLVDARVDGVQLLD
                     LRTKTAKVPEGIPTIEEDPNARVILINDIPEDFKTWRNQFVHKDFDAIYFKNQMKHPY
                     YLTGFGSREQFAKLYKTIYQFPEFDLR"
     gene            complement(1951..2715)
                     /locus_tag="VGT87_001433"
     CDS             complement(1951..2715)
                     /locus_tag="VGT87_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEQ3814271.1"
                     /translation="MAQRIIVITGASGGLAQAIVKQLPKEDSLILLGRNKERLEHCYQ
                     HIDNKECLELDITNPVAIEKMVAQIYQRYGRIDVLINNAGYGAFKGFEEFSAKEIADM
                     FQVNTLASIHFACLIGQKMADQGQGHLINIVSMAGLIASAKSSIYSATKFALIGFSNA
                     LRLELADKGVYVTTVNPGPIATKFFDQADPSGHYLESVGKFTLQPNQVAKRLVSIIGK
                     NKRELNLPFSLAVTHQFYTLFPKLSDYLARKVFNYK"
     gene            complement(2715..3644)
                     /locus_tag="VGT87_001434"
     CDS             complement(2715..3644)
                     /locus_tag="VGT87_001434"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEQ3814272.1"
                     /translation="MELQFLGTGAGQPAKQRNVSSLALKLLDEINEVWMFDCGEGTQR
                     QILETTIKPRKIRKIFITHLHGDHIFGLPGFLSSRSFQASEEQTDLDIYGPIGIKTYV
                     LTSLKVSGARVPYQIHFHEFDDKSLGKIMETDKFEVYAERLAHTIFCMGYRVVQKDLE
                     GTLDAEALKAAGVPFGPLFGKIKNGQDVELEDGRLICAKDYISAPKKGKIITIIGDTR
                     KTSASVKLAKDADVLVHESTYGKGDERIARNHGHSTNMQAAQIAHEAGAKRLLLNHVS
                     ARFLGRDCRQMEKDAATIFENVKMVQDLEEVII"
     gene            complement(3659..4294)
                     /locus_tag="VGT87_001435"
     CDS             complement(3659..4294)
                     /locus_tag="VGT87_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEQ3814273.1"
                     /translation="MNNYIDLAKTYGGFTSLDTNYLNHLLASLTDQQKLAFITPPPSV
                     INAYFAEIYQKQSPQAATDYYFNLSKALGLFTDQPSFEEEKPFVRLNLSGKAYGFAYQ
                     NDQEVALVFSEKAEPKKPELFFELTQIFPQYMVYEDKGQLKMQAKQFEQGECEDITPD
                     DTLLSKIYRLANGITMLKGFNVEELWALSQTFSGQKYYDFAQREFMIYITQ"
     gene            complement(4287..5525)
                     /gene="hflX"
                     /locus_tag="VGT87_001436"
     CDS             complement(4287..5525)
                     /gene="hflX"
                     /locus_tag="VGT87_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEQ3814274.1"
                     /translation="MIETKRQQERVILLGVELQTTEHFDMSMTELANLAKTAGVKVMA
                     SFSQKRERYDSKTFIGSGKLDEIKAIVEADEIDAVIVNNRLTARQNANLEAVLEVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYMLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSIRHQIADIERQLTQVEKNRQTIRDRRVGSDTFKIGLIGYTNAGKSTIMNLL
                     TDDSHYEANELFATLDATTKQLYLENQFQATLTDTVGFIQDLPTELVAAFKSTLEESK
                     YVDLLLHVIDASDPNHSEQEKVVLNLLKELDMLNIPRLAIYNKVDIAEQFTATAFPNI
                     RISARSKDSKILLRRLIIDQIRDQFVPFRIKVHQDKAYKLYDLNRVALLDHYTFDQEI
                     EDISGYISPKQQWRLDDFYE"
     gene            complement(5598..6497)
                     /gene="miaA"
                     /locus_tag="VGT87_001437"
     CDS             complement(5598..6497)
                     /gene="miaA"
                     /locus_tag="VGT87_001437"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEQ3814275.1"
                     /translation="MTKIKIVVIVGPTAVGKTALGISLAKAFNGEIISGDSQQVYRQL
                     DIGTAKATQEEQEAAVHHLIDIREVTESYSAYDFVQDAQKAISDIVSRGKLPIIVGGT
                     GLYLQSLLEGYHLGGQVDQEAVKAYRNELEQLDDHDLYERLQVNNITIEQVNRRRAIR
                     ALELAQFADELENAETAYEPLIIGLNDDRQVIYDRINQRVDRMLENGLLEEAKWLYEH
                     YPTVQASRGIGYKELFPYFVGEMTLAEASDQLKQNTRRFAKRQLTWFRNRMAVSFTAI
                     TAPDYPQVVHDRVRDFLGQKEKS"
     gene            6631..6807
                     /locus_tag="VGT87_001438"
     CDS             6631..6807
                     /locus_tag="VGT87_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002263068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEQ3814276.1"
                     /translation="MVKKYQFVKGLATGVLATAATVAGAVFAVKKTIIDPEEEKAAFI
                     EENRKKAARRRVAR"
     gene            complement(6925..7833)
                     /locus_tag="VGT87_001439"
     CDS             complement(6925..7833)
                     /locus_tag="VGT87_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEQ3814277.1"
                     /translation="MKHLKNPPLVMSGLALGTLSFGNLLATYVSIFSYLGILAALFIY
                     GILLVGMVRNLNDTKMQLRQPLIASVFPTFFMTGMLLSSLFLKVTGGCWLGFLTWWLF
                     FLGNLVLIAYYQYRFVFSFSWDNVFPSWSVLFVGIAMAALTAPASRQFLLGQVIFWVC
                     LLLTAVILPFMAKKTYGIGLGQAVMPNISTFCAPLSLLSASYLATFPRPQVGMVIFLL
                     ISSQLLYAFVVVQLPRLLNRPFNPGFSAFTFPFVISATSLKMTLSFLGWQGLGWQVLL
                     LGEVLLATALVTYVYGAYLRFLFQNK"
     gene            complement(7892..8278)
                     /locus_tag="VGT87_001440"
     CDS             complement(7892..8278)
                     /locus_tag="VGT87_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEQ3814278.1"
                     /translation="MLFIAKQTLKSQLIPQDNLLAESRFNEIMDFLTGDFPLVFRPMY
                     NPHRYTISQDNQALEKVKQASYKRMDIAMTHLDGLIGDSGHVYRDQQTIADAYAYAMA
                     LWSQKTPKSYENYPHLAAFMAKMLEM"
     gene            complement(8589..>9541)
                     /locus_tag="VGT87_001441"
     CDS             complement(8589..>9541)
                     /locus_tag="VGT87_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEQ3814279.1"
                     /translation="YVSIENAQEYAAKHLEFCKSIGLKGRILIADEGINGTVSGDYET
                     TQKYMDWVHSDERFADLWFKIDEENQQAFRKMFVRYKKEIVHLGLEDNNFDSDINPLE
                     TTGEYLNPKQFKEALLDEDTVVLDTRNDYEYDLGHFRGAIRPDIRNFRELPQWVRDNK
                     DKFMEKRVVVYCTGGVRCEKFSGWMVREGFKDVGQLHGGIATYGKDPEVQGELWDGAM
                     YVFDDRISVPINHVNPTVISKDYFDGTPCERYVNCANPFCNKQIFASEENETKYVRGC
                     SPECRAHERNRYVQENGLSRQEWAERLEAIGESLPEFVGA"
BASE COUNT         2829 a         2082 c         1778 g         2852 t
ORIGIN      
        1 gccgtaaatc aaattcagga aactgataga tggttttgta gagcttggca aactgctctc
       61 tgctgccaaa tcctgttaaa taataaggat gtttcatctg atttttaaaa tagatggcgt
      121 caaaatcctt gtgaacaaac tggttgcgcc aggttttgaa atcttcaggg atgtcattaa
      181 tcagaatgac tctggcattt gggtcttctt caatagtcgg aataccttca ggaactttgg
      241 ctgtttttgt ccttaaatca agaagttgca ctccgtcaac acgagcatct actaacatga
      301 attggagact cgtgttccca ttccaatggt tgacagataa ggtaactgct aattctaaac
      361 cagtagcttg tcgaaactct tgcagttgac ttccttgacc aaaagccagt acgtcaaagg
      421 aagctttgcc ctgactgact ttaaatttaa gatggctctg gtcttgccct atggtacgcg
      481 cttgactaac ccttatgtcc ttgacataaa agaccggctt ttgatgatcc atcccataag
      541 gggctaattt atctaaactc tttaaaatat caaggctaag gtcgtccaaa gacaggcttt
      601 catcaatagt taacgtattt ttggcagtct gatctaagcc acgttctatc acaaactgac
      661 aaagaaagtc tgaaagagct tccaagttgt caacgggcag ggtcattcct gctgcaccag
      721 catgtccccc aaaggctgta aataactcac gcttgccatt caaggcttca aaaatattaa
      781 tagcttctaa acttctggcc gagccttttg caaagccatt atcaatggtc aaaacgacga
      841 ccgtttgtcc aatagtttcc atgatacgac cagcgacaat cccaagaaca ccaggatgcc
      901 agccagcttg tgccaagact tgaacaggtt tttgaggatc aaccatagcc atcgcttgat
      961 caaaaatgtc ttggactaag gccttacgct cttcattttt tttcttaatc atcaaggcaa
     1021 tcgcttgagc ttcttggtca tcaaagcccg tcagtagctc aatggctgga ttagggtcat
     1081 ctaagcgccc taaagcattt aattgcggag caatctgaaa gccaatggcg tcttcattaa
     1141 aatgttccaa atcaatagga gacaaggaca tcaactcctg caagcccact ctttcggtgt
     1201 gtttgagcat ggccagacca tttttgacta aaacacggtt ttcacctgtc aagcttacca
     1261 tatcggcaat ggttccaata gctaccaagt ctagacagtc tgttggtaaa ctttctagca
     1321 aagctgttgc caatttaaag gcaacgccac agccagctaa gtgtttaaag ggataatcag
     1381 catcaggatg ctcaggatga atgatggcaa aggcttctgg caattcttca ggcaggctat
     1441 gatggtcagt tacaatgaca tcaacttcct gttcttgagc atatctaatc gcttcatgtc
     1501 cggccacgcc attatcaacc gtgataatta gggacacagc ttcctgctca ataaaatatt
     1561 tatagacgct ctggttgggg ccatagccat ctgtaaagcg gttgggaaga taaaccaaag
     1621 gctctgctcc catcatgtct agggtttctt tgacaataga agccgacgtc atgccatcag
     1681 catcatagtc gccgtaaatg agaattcgtt cgccctcttc aatagcttga cgaattcttg
     1741 gcacagcttt tgtcatgtca tgtagcaagt aaggatcatg cagttgagac aagtccgctg
     1801 ttaaaaactc atctaaggct tcttcagtcc ttatccctcg atcataaatc aactgagcgg
     1861 cggtctgggt tagacctttt gtctttgcta gcttaaagaa gccatcatct ggcttcttgt
     1921 cttttatttt ccagctatat ttggatttaa tcatttataa ttaaatacct ttcttgcaag
     1981 ataatcagat aatttaggga aaagggtgta aaattgatgg gtcaccgcta aactaaaggg
     2041 caaattcaat tctcgtttat ttttcccgat aatagaaacc aaacgcttag ccacttgatt
     2101 tggttggaga gtaaatttac caacgctttc caaataatgt ccagacgggt cagcttggtc
     2161 aaaaaacttg gtggcaatgg gacctggatt cacagtggtc acgtaaaccc ctttatccgc
     2221 taattctaag cgaagggcat tggaaaatcc gataagggca aacttggtgg ctgaataaat
     2281 actcgatttg gctgacgcaa tcaagcctgc catggacaca atattaatga gatggccttg
     2341 cccctgatct gccattttct gaccaatcaa gcaagcaaag tgaatgctcg ctagggtgtt
     2401 aacctgaaac atatcagcta tttctttggc agaaaactct tcaaagcctt tgaaagctcc
     2461 atagccagca ttattaatca agacatcaat acggccatag cgctggtaaa tctgggcgac
     2521 cattttctca atggctactg gattggtaat atccaactca aggcattctt tgttgtcaat
     2581 atgctgataa cagtgttcta ggcgttcttt gttacgtcct aataaaatca agctgtcttc
     2641 cttgggtaac tgcttaacaa ttgcctgagc cagtcctcca gaagctcccg tgataacaat
     2701 gattctttgt gccattaaat aatcacttcc tctaaatctt gtaccatttt aacattttca
     2761 aatatagtgg cggcatcttt ttccatctgg cgacaatcac gccctaaaaa gcgagctgat
     2821 acatgattga gcaagaggcg ttttgcgcct gcttcatgag cgatttgtgc tgcctgcatg
     2881 ttggtagaat gcccgtgatt tctcgcaatc cgctcgtcac ccttaccata ggtggattca
     2941 tgaaccaaaa catctgcatc tttagcgagt ttaacactgg cagacgtttt gcgcgtatcg
     3001 ccgataatgg tgatgatttt gcctttttta ggggcagaaa tataatcttt ggcacaaatc
     3061 aggcggccgt cttccaactc aacgtcttga ccatttttaa ttttgccaaa gagtggtcca
     3121 aaaggaacac ccgctgcttt taaggcttct gcgtctaggg ttccttctaa gtctttttga
     3181 actactcgat aacccatgca aaaaatggta tgcgccagac gctctgcata gacctcaaac
     3241 ttatctgttt ccattatttt acctaatgat ttgtcatcaa attcatgaaa atgaatctga
     3301 taaggtacac gcgcaccaga aacttttaga ctagttaaca cgtaagtttt aatgccaatt
     3361 ggtccataaa tatcaagatc tgtctgttct tcactagctt gaaaagaccg acttgataaa
     3421 aatcctggta atccaaaaat gtggtcccca tgcaaatgag taatgaaaat ctttcttatt
     3481 tttcgtggct taatcgtcgt ttctaaaatt tgtctttgcg ttccttcacc gcaatcaaac
     3541 atccagactt cattgatttc gtctagtaat ttcaaggcaa gacttgacac attgcgctgc
     3601 ttggctggtt gaccagctcc agttcctaaa aattgtaatt ccattcaatc tattctttct
     3661 attgggtgat gtaaatcata aattctcgtt gggcaaaatc ataatacttt tgaccagaaa
     3721 atgtttggct aagagcccat aactcctcta cattaaaccc cttaagcatg gtgataccgt
     3781 ttgccagacg gtaaatcttc gacaagagtg tgtcatcagg cgtaatgtct tcacattctc
     3841 cttgctcaaa ctgcttagcc tgcattttaa gctggccttt gtcttcataa accatatatt
     3901 gtgggaaaat ttgtgttaat tcaaagaaga gttcgggttt cttaggctct gctttttcag
     3961 aaaataccaa tgcaacttct tggtcatttt ggtaagcaaa accataagct tttccagaca
     4021 aattgaggcg tacaaagggc ttttcttcct caaaagatgg ttggtcagta aaaagcccca
     4081 aagccttaga aagattaaaa taataatcag tcgcagcttg cggtgactgt ttttggtaga
     4141 tttcagcaaa gtaggcatta ataacacttg gtggtggtgt gataaaagct agtttttgct
     4201 ggtctgtcaa actggctaat agatggttta ggtagttggt atccagacta gtaaatccac
     4261 cataggtttt tgctaaatca atataattat tcataaaaat cgtcaagtct ccattgctgt
     4321 tttggggaaa tgtagcccga aatatcctct atttcttggt caaacgtgta gtggtctaaa
     4381 agagcaactc ggttcaaatc atacagcttg tacgccttgt cttgatgcac tttgattcta
     4441 aaaggaacaa actggtctcg aatttggtca atgattaaac gtctcaagag aattttgcta
     4501 tcctttgaac gggctgaaat cctgatattt ggaaaagcag tagctgtgaa ttgttctgct
     4561 atatccacct tgttataaat agctaaacgt ggaatattaa gcatgtctag ctcttttaga
     4621 aggtttaaaa ccactttttc ttgctcacta tgattgggat cactcgcatc aatcacatgt
     4681 aacagcaaat ccacatattt gctttcttct agggttgatt taaaagcagc cactaattcc
     4741 gttggcaagt cttgaataaa gcctacggtg tctgttaagg ttgcttgaaa ttgattttct
     4801 aaatagagtt gctttgtagt tgcatctaag gtagcaaata attcattggc ttcatagtgg
     4861 ctgtcatctg tcaacaagtt cataatggtt gacttgccag cattggtgta gccaatcaag
     4921 ccaattttaa aggtatctga acccacacga cgatctctaa ttgtttgacg atttttctct
     4981 acctgagtta attggcgttc aatatcagca atctgatgac gaatcgaacg gcgatttaac
     5041 tctagttggc tttcacctgg tccacggctc ccaataccac ctgcttgacg acttaacatg
     5101 atgccctgtc caaccaaacg aggtaacata tatttcaatt gggcaaggtg tacttgtaat
     5161 ttgccctcat ggcttctggc acgcatggca aaaatatcta agattaactg catacggtca
     5221 atgactttta cctctaaaac agcttctaga ttagcatttt gcctagcagt caaccgattg
     5281 ttcacaataa ctgcatcaat ctcatcagct tccacaatcg ccttaatctc atccagtttc
     5341 cctgagccga taaaggtttt gctgtcgtaa cgttctcgtt tttggctgaa acttgccata
     5401 actttgacgc cagctgtttt ggcaagatta gctaattctg tcatggacat atcaaaatgt
     5461 tctgttgtct gaagctcaac tcctaaaaga atcactcttt cttgctgacg ctttgtctca
     5521 atcatcacct ttaccaatcc tccaaattcc gtaaattcta ttataacata tcttcaacag
     5581 attctgctaa ttttgcgtca tgacttctct ttctgaccaa gaaaatccct gaccctatca
     5641 tgtactacct gtgggtaatc aggagccgtt atggcagtga agctaacagc cattcgattg
     5701 cggaaccaag ttagctgtcg cttggcaaaa cgtctcgtgt tttgttttaa ctgatcacta
     5761 gcttctgcca aagtcatttc acctacaaaa taggggaata attccttata acctatgcca
     5821 cgactagcct gaacagtcgg gtaatgctca tataaccatt ttgcttcttc caataagccg
     5881 ttttcaagca tacgatccac gcgctgattg atacggtcat agatgacttg gcggtcatca
     5941 ttcaagccga taataagcgg ctcataagcc gtctcggcat tctctaattc atcagcaaac
     6001 tgagccaatt caagggctcg gatcgcccgc ctgcgattca cctgctcaat ggtaatgtta
     6061 ttgacttgca agcgttcata taagtcgtga tcgtccaatt gctccaactc attccgatag
     6121 gcttttaccg cctcttgatc aacttgacct cctagatgat aaccttcgag taaactttgt
     6181 aagtaaagac cagttccacc tacaataatt ggtaatttcc cacgactaac aatgtctgaa
     6241 atagcttttt gagcatcttg aacaaaatcg taggccgaat aagactccgt cacctcacga
     6301 atatcaatta agtgatgaac cgctgcttct tgctcttctt gagtggcctt ggctgttcca
     6361 atatccaact gtcgataaac ttgctggcta tctcctgaaa taatttctcc attaaaagcc
     6421 ttggctaaac taatcccaag tgctgttttt ccaaccgctg tcggaccaac aatcacaact
     6481 atttttattt ttgtcatttt cttgtctttc tattagaaaa gcgtgtccaa aaacgccttt
     6541 tacttgaaaa ttagcttatt tttcgctacc atatattata acataagatt aaggaccact
     6601 gaggtccttc actagaaatg gagaataatc atggttaaga aatatcaatt tgttaaaggt
     6661 cttgctactg gcgtcctagc aactgctgct actgttgctg gtgctgtctt tgctgtaaaa
     6721 aaaacaatca ttgaccctga agaagaaaaa gctgctttca ttgaagaaaa ccgcaaaaaa
     6781 gctgcgcgtc gtcgtgtagc tcgctaacgt gacaatggat caatttaaaa gagactagca
     6841 cattaaagtg ccggcctctt ttttagtaat ctcgataact agttagtagt aactgtctct
     6901 tgatgagcca agatgttagc tgctttactt attttgaaat agaaatctca ggtaagcccc
     6961 ataaacatac gtgactaaag ctgtcgccaa caatacctct cctagcaaaa gaacctgcca
     7021 acctaaacct tgccaaccta aaaaagataa ggtcattttt aaggaagtgg cactaatcac
     7081 aaacggaaag gtaaaagctg aaaaacctgg gttaaaggga cgattgagta agcgaggaag
     7141 ctgtacaacc acaaaagcat ataacagttg tgagctgatg agaagaaaga taaccattcc
     7201 tacttgtggt ctaggaaagg ttgctaaata agacgctgaa agtaaggaca gaggggcaca
     7261 aaacgttgaa atattgggca ttactgcttg acctaaacca atcccataag tctttttggc
     7321 cataaaaggt aaaatcacag cagttaagag aagacaaacc caaaaaatca cctgtcctaa
     7381 taaaaactgc ctactagcag gtgctgttaa tgccgccatt gcaattccaa caaaaagcac
     7441 ggaccaggaa ggaaaaacgt tatcccatga aaaagaaaag acaaaacgat actggtaata
     7501 agcaatcagg acaagatttc ccaaaaagaa tagccaccaa gtgagaaagc ctaaccaaca
     7561 ccctcctgtc actttgagaa agagtgaaga caacaacatc cctgtcataa aaaaagttgg
     7621 aaaaacagag gcaattagag gttgtcgtaa ctgcattttg gtatcgttaa ggttcctaac
     7681 catgccaaca agcaagatac catagataaa aagagctgcc agaattccca aataactaaa
     7741 aatcgaaaca taagtagcca acaaattacc aaatgacaac gttcctaatg ccaatcctga
     7801 catgaccaat ggtgggtttt taaggtgttt cataagtgtc cttttctgcc tattaacagc
     7861 tatttttagt gacataatct tgtttatttt atcacatttc gagcattttt gccataaagg
     7921 ctgccagatg cggataattc tcgtaagatt ttggtgtttt ttgagaccat agcgccatgg
     7981 cgtaagcata agcatctgca atggtttgtt gatctcgata aacatgccca ctatccccaa
     8041 taagaccatc taaatgcgtc atagcaatat ccatccgctt gtaagaagct tgtttgactt
     8101 tttcaagcgc ttgattgtct tgagaaatag tgtagcgatg agggttgtac attggcctaa
     8161 aaacaagggg aaaatcacct gttaagaaat ccatgatttc gttgaatcga ctttctgcca
     8221 aaagattatc ttggggaatg agctgagatt tcagggtttg tttcgctata aagagcaaga
     8281 taatacctgc ctgtgtgacg atttgaccat catcaagctg taaagcttaa accattccca
     8341 aaggattgat ggtaaggcag tcttcagaat ccagttttac cttctctatc tcgtaataca
     8401 gaaactcaca attcttttga aaaggttcta gacagagttg gtgttgacgt cttcgctgtt
     8461 gatggctctg catgacaaaa tgagcttggt gtccacgttc actagtatct tgttgcaaag
     8521 tctttgcaat ttggtctcat catctgtatc acaaaaaaca aaccagtttc ctgatttgtt
     8581 ttgagatatt aggcacctac aaattctggc aaggattctc caatagcttc taaacgttct
     8641 gcccattctt gacgtgataa accattttct tgcacatagc gattacgttc atgtgcacga
     8701 cattcaggcg agcatcctcg aacatacttt gtttcgtttt cttctgaagc aaaaatttgt
     8761 ttgttacaaa acggatttgc acaattgaca taacgttcac aaggtgttcc atcaaaataa
     8821 tctttactaa tgacagttgg attaacatga ttaattggca ccgagatacg atcatcaaaa
     8881 acatacatcg caccatccca aagttcacct tgaacttccg gatcttttcc atatgttgca
     8941 ataccaccgt gtaattgtcc aacatcttta aagccttctc gaaccatcca acccgagaat
     9001 ttctcacagc gaacaccacc cgtacagtac acaacaacac gtttttccat aaacttatct
     9061 ttgttgtcac gtacccattg tggtaactca cggaaattgc gaatatcagg acgaattgct
     9121 ccacggaagt gacctaagtc atactcataa tcattacgcg tatctaaaac aacagtatct
     9181 tcatcaagca aggcttcttt aaattgttta ggatttaaat actcacctgt tgtttccaaa
     9241 ggattaatat cactatcaaa gttattgtct tctaacccaa ggtgcacgat ctctttctta
     9301 taacgaacaa acattttacg gaaagcttgt tgattttcct cgtcaatctt gaaccaaaga
     9361 tcagcaaaac gctcatcgct gtggacccaa tccatatact tctgagttgt ttcataatct
     9421 ccagaaacag ttccattaat cccctcatcc gcaatcaaaa tacgaccttt aagcccaata
     9481 gatttacaaa attctagatg tttcgcagcg tactcttgcg cattttcaat tgaaacatat
     9541 t
//
LOCUS       DAUCLR010000072         9292 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.072, whole genome shotgun sequence.
ACCESSION   DAUCLR010000072 DAUCLR010000000
VERSION     DAUCLR010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9292
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.072"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..5060)
                     /locus_tag="VGT87_001442"
     CDS             complement(<1..5060)
                     /locus_tag="VGT87_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004818439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEQ3814280.1"
                     /translation="MQVNDFKNIQEAIKYEVLQDEKEYLKLLKVIGNNQKYDFSSQLS
                     IYNKEPEARACATFDMWKKYFGRVVMRGEKGIPILVGSDINQRVSYIFDISQTTSMDR
                     NINEVSLWQFDHENHNEALKEIINNSSFEASDSLNENIFSLSRIYGDEYINLALADLR
                     IDIEDRLSFEKFMRDSISYAVANRFNTVYPMDMENLKNNFSRINTISLEQIGLVISRV
                     SEDIIDSTIEKSKEIDRARLLTERDAADYNKDIENINEDRGGQDDLYRRDDRSRSRDG
                     RVFTDGSDRGNSDEDRRESLGYDGEGSGIYGEISESDLRSPKTVLPSRERGHGELEET
                     SGNVRGENTFEPSEGNSRSGSGLYQERESQDDESTRIDREDDERESRGIPRTDEQLDG
                     NSEENGNQGIRGSLENEISQEKEADEASFFDGNNIEKRKDYWIVEFNENHELVPDYSG
                     QIVTKELINVLRQKDIDVKDHNQTLGENEFGEMTDDYIGYFKFYFDHYVDGEVVEHYR
                     IDLGDGEEVNEREFSYLEDQVALSEEKSFQEEVKEKIEPKFKIGDQVRYKDKDFTITD
                     FDELSGGLKTVTIRDNMEYMGGMIRGSEVIPYRNDSYLEEIFENLSQTSEKLAVKVGK
                     EFILEDENTFNGIKLIETGTKVEVNGEEIPLYKAETFEESRKIDDLLDSGNYEIYKLS
                     EHENQIERQVEQESFIDTHNPEIDQMMDRYDVPREAAENLLRGKEDLKNLGYEPNKER
                     LSFARNYDLKNHIYSEYLTPSEKLDKNIKAIKMLKRLENENRSPREYEQAYLADYLGW
                     GGLADVFDEEKGGQWLEARNILKENLTNEEYLNAKESTLTSFYTPREVMDGIYKTLTD
                     MGFKTGNILEPSAGVGNFIGNMPSEIQGSKVYGVEKDSLSGRIARELYPEANIQIKGF
                     EETNFSNNFFDLVIGNVPFGDFKVNDREYNRNNFLIHDYFFAKSIDKVRNGGIIAFIT
                     SSGTMDKKDESVRKYINARCEFLGAMRLPNTTFKGLAGTEVTSDIIFLKKRDSVIERD
                     DDWIHLATDKKGLTYNKYFVDNPQMVLGDMKEVSGRFGNTITCDEKEDEKLKDLMDLA
                     SKEISSNSKYEEVELLEDEELSLPATDDVKNFSYTIIDEEVYLRENSVLIKQKISDKN
                     KEKIKDYLDVMNALKDVIEKQKDDFSDEEIKESQAKLNEVYDNFSKKHGYINSLSNTR
                     ALKEDSNFPLVSSIEILDDEDNFKAKSDIFSKRTITKAKVVDHVDTSLEALVLSVSQK
                     GYVDFEYMESITSKDRDTLIGELEGEIFLDIKDTDLINNRMPFENFNNDDPFHFSYVS
                     ADEYLSGNIREKIGYLNSYIGEIENVIDLAPSEKKDTLLNELGKLKYQREKLQEVMPE
                     ELTASDINVRLGATWIPQKDIEDFTFNLLKTPGYDRWNINVRFSPHTSEWNIEGKSVD
                     STNDLANMTYGTSRVNAYKLIENALNLKDTKVFDQVINDDGSKASVLNKKETMLASQK
                     QELIKEEFKNWIFEDPDRRYRLEKIYNEKFNSIRNREFDGSNLTFDGMNTEIKLREHQ
                     KNAIARTLYGGNTLLAHVVGAGKTFEMVASAMESKKLGLASKSLFVVPNHLTTQIGRE
                     FMQLYPSANIMVADKKDFQPKNRKRFIGRIATGEYDAVIIGHSQFEKIPMSKEYQVRH
                     IQDQIDDIVSFIDENKRN"
     gene            complement(5047..6012)
                     /gene="dcm"
                     /locus_tag="VGT87_001443"
     CDS             complement(5047..6012)
                     /gene="dcm"
                     /locus_tag="VGT87_001443"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEQ3814281.1"
                     /translation="MNKVKILELFGGIGAIRKAFINLKIPYEVVDYVEIDKACVKSYN
                     ALYGEDYKPKSVVGYKIPNEKIDLIMHGSPCQDFSRIGKKQGGVKNSGTRSSLLFETI
                     RIIKEMKEKPKWIIWENVKGVLDRNMRDSFFIYLKELEDLGYESKYEILNAMDFGIPQ
                     KRERIFVVSCLGANNFSFNKLERKETRPLSEFLEKDVSELYTMTQPYMLKFLNKGIDN
                     SFKGRLKVIKDFSYTISTKQMRVPNSGIIDIGNGQYRYLTERECLRLMGFDDSDIDKL
                     EKVHPRRKNCTSSKLYKQAGNSIVVDVLMAIIKEIHRMEVGNASK"
     gene            complement(6005..7726)
                     /locus_tag="VGT87_001444"
     CDS             complement(6005..7726)
                     /locus_tag="VGT87_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HEQ3814282.1"
                     /translation="MKLVIAEKPSVAVTIAKVIGARTRKNGYYEGNGYIVSWCVGHLI
                     QMASPERHDEKWKKWTIDTLPIIPEEYIYEVSKSTKKQYGVLKKLLNDKNIDTVINAC
                     DAGREGELIFRLVYNQAKCKKKIQRLWISSMENKAIEDGFRNLKDGENFEDLYRSASA
                     RAIADWLVGMNLSRLYSCIYKETYSVGRVQTPTLYLIAKRDSEINLFKKQKYYTVDLS
                     YGGLKLVSDRIDKIEVAEQLLNLLEDEIVITEVEDKDISTKPDKPYDLTTLQREANKY
                     FGYSANDTLNLAQGLYEKKLITYPRTDSRYLTDDMVNTMKELLEGLEDDFKINESNFK
                     SIFNSSKVTDHYAIIPTISGIGKAKDLSDKESKIYNLIKDKLLASCSDNLKESSRKTR
                     YEYDKFNFNASGKTVTDEGYTKYLKSYVKERQENELPDIKTGDKIKLTSKNISEKFTK
                     APSHYNEDTLLKAMENAGVETLDKDIEVERKGLGTPATRAGIIENLIHKDLIRRDKKN
                     LLVTEKGNRLVSIVEDKFKSAETTSEWEMKLAKISYGEVDKEDFLREIEDSIRELVDR
                     YKNNLNE"
     gene            complement(7818..8801)
                     /locus_tag="VGT87_001445"
     CDS             complement(7818..8801)
                     /locus_tag="VGT87_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002834743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEQ3814283.1"
                     /translation="MSKFYIENKEDLRVLIVNTARKKNISEAVIEKDYWVTFILDYLF
                     NENKWREYFTFKGGTSLSKCFGLIERFSEDIDLILDWRVLGYEEKEPWLERSNTKQDK
                     FNKEVNEKTEIFLRDELLKVLEQDFKDMDFEFMIDTLDPQTILCKYPKIFESNYLTQN
                     IRLEIGSLAAWTPAIEVEILPIIGEAYPNVFKEKTNIRTVSAERTFWEKATILHHEAN
                     RPESSPMPHRYARHFYDLYKIANSDFKDRALEDKELLKKVTEFKMKFYPRKWARYEEA
                     LDGRLKLVPRKYRFSEIEKDYKAMEEMIYGEYPKFDEIIKVLKELEKEINK"
     gene            complement(8794..>9292)
                     /locus_tag="VGT87_001446"
     CDS             complement(8794..>9292)
                     /locus_tag="VGT87_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814284.1"
                     /translation="LNQLVSENKITRVINGFYYNPRYSELIGEYEAVSIHELALAIAR
                     KYNWNIAPYNSTALNLLGLSTQVPTHYKYISSGRYKEYKIGDTVLEFKKVNPGEIANM
                     SLKTATVIQAIKSLGKENINAQVIQKIRENLSEKERIDLMNESKSVPAWIYEVIREIC
                     EGKNE"
BASE COUNT         2573 a         1829 c         1126 g         3764 t
ORIGIN      
        1 ctatttcttt tgttctcatc aataaaggaa actatatcat ctatttggtc ttgtatatgt
       61 ctaacctggt attccttaga cattggtatt ttttcaaatt gagagtgtcc tataatgact
      121 gcgtcatatt ctcccgttgc aatcctacca ataaatcttt tcctattttt aggttgaaag
      181 tcttttttat cggccaccat aatatttgct gacggatata actgcataaa ctctctacca
      241 atttgagtgg ttagatgatt gggaacaaca aataatgatt tacttgcaag tcctaacttt
      301 ttagattcca tagcagaagc taccatttca aaagtttttc ctgctcctac tacatgggct
      361 agtagtgtat ttccaccata aagagtcctt gctatagcat ttttttgatg ttctcgaagt
      421 ttgatttcag tattcattcc atcaaaagtt aagttagagc catcaaattc tctatttcta
      481 attgaattga atttttcatt ataaattttt tctagcctat atcttctatc aggatcttca
      541 aatatccaat tcttaaattc ttctttaatc aattcttgct tttgacttgc aagcatagtt
      601 tcttttttgt ttaatacaga ggccttagaa ccatcatcgt ttattacttg gtcaaatacc
      661 tttgtatctt ttagattaag ggcattttca attagcttat aggcatttac tctacttgta
      721 ccataagtca tgttagcaag gtcattagtc gagtcaacac ttttaccttc aatgttccat
      781 tcacttgtat gtggtgaaaa cctaacattt atattccacc tatcatagcc tggtgttttt
      841 aaaagattaa aagtaaagtc ttctatatct ttttgaggta tccaagttgc tcctaacctt
      901 acatttatat cagaagcagt gagttcttca ggcataactt cttgtaattt ttctctttga
      961 tatttgagtt tgcctaactc gtttaataat gtgtctttct tttcagaagg tgctaaatct
     1021 attacatttt caatttctcc gatatatgaa ttgagatagc caatcttttc tctaatatta
     1081 ccacttaaat attcatcagc cgatacatat gaaaaatgaa atggatcgtc attgttaaag
     1141 ttttcaaagg gcattctgtt atttattagg tctgtatcct taatatctaa aaatatctcg
     1201 ccctcaagtt caccaattaa ggtgtcccta tctttacttg tgatagattc catatattca
     1261 aaatctacat atcccttttg tgaaactgat aagactaagg cttcaaggga agtatctaca
     1321 tggtctacta cttttgcctt tgttattgtt ctttttgaaa atatatcact cttagctttg
     1381 aaattatcct catcatcaag tatttctatt gatgaaacca aaggaaagtt tgagtcttcc
     1441 tttaaggctc tagtatttga taaggagtta atatagccat gtttctttga aaagttatca
     1501 tagacttcat ttaactttgc ttgtgactct tttatttcct cgtcagaaaa atcatccttt
     1561 tgtttttcta ttacatcttt caaagcattc attacatcaa gataatcttt tatcttttct
     1621 ttgtttttat ctgatatttt ctgcttaatt aagactgagt tttctcttag atagacttct
     1681 tcgtcaatga tggtatagga aaaattctta acatcatctg ttgctggaag acttaattct
     1741 tcatcttcca atagctcgac ttcttcatat tttgaatttg aagatatttc cttacttgca
     1801 agatccatta aatcttttaa cttttcatct tctttttcat cacaagtaat agtatttcca
     1861 aatctaccag atacttcttt catatcccct aataccattt gaggattatc tacaaagtat
     1921 ttgttgtaag ttaaaccctt tttatcagtt gctaaatgaa tccaatcatc atctctttct
     1981 ataactgaat ctctcttctt tagaaaaata atatctgaag taacttctgt gccagcaagt
     2041 cctttaaatg ttgtattagg aagcctcata gctcctaaaa actcacatct tgcattaata
     2101 tattttctaa cagattcatc ttttttatcc atagttccag atgatgttat gaaggcaata
     2161 attcctccat tcctaacctt atctattgac tttgcaaaaa aataatcgtg aatcagaaag
     2221 ttatttctgt tatattcacg gtcgttaact ttaaaatctc caaagggaac atttcctata
     2281 actaagtcaa agaaattgtt tgagaagttt gtttcttcaa agcctttaat ttgaatgtta
     2341 gcttcaggat aaagttctct tgctattctt ccgcttagac tatccttttc tactccatat
     2401 actttagagc cttgtatttc acttggcata ttaccaataa aattaccgac ccctgcagat
     2461 ggttcaagga tattcccagt cttaaaaccc atatctgtta gagtcttata tattccatcc
     2521 attacctctc taggtgtata aaaggatgtt aaagtagact ctttagcatt caaatactct
     2581 tcatttgtta gattttcctt taaaatattc cttgcttcaa gccattgacc tcccttttct
     2641 tcatcaaaga catcagcaag accaccccaa cctagatagt cagcaagata ggcttgttca
     2701 tactctcttg gactcctatt ttcattttca agtcttttga gcattttaat agctttgata
     2761 tttttatcta acttttctga tggtgttagg tattctgagt aaatatgatt tttcaagtca
     2821 taatttctag caaaacttag cctttcctta ttaggttcat agcctagatt ttttaaatcc
     2881 tctttacccc tcaataagtt ttcggctgct tcccttggga catcatacct atccatcatt
     2941 tggtcaattt ctggattgtg ggtatcaata aagctttctt gctctacttg tctttcaatt
     3001 tgattttcat gctcagataa tttgtatatc tcatagtttc cactatctaa aaggtcatca
     3061 atctttctac tttcttcaaa tgtttcagcc ttatataagg ggatttcttc tccattaact
     3121 tctaccttag ttcctgtttc aattaattta attccattaa aggtattctc atcttctaaa
     3181 ataaattcct taccaacttt tactgctagt ttttctgatg tttgacttag attttcaaag
     3241 atttcttcaa ggtatgaatc gtttctatat ggaattactt ccgaacccct aatcatacct
     3301 cccatatatt ccatattatc tctgatagtg acagttttaa gtcctccact tagttcatca
     3361 aaatctgtaa tggtaaagtc cttatcttta tatctaacct gatcgcctat cttaaattta
     3421 ggttctatct tttccttaac ttcttcttga aaagattttt cctcacttag tgcaacttgg
     3481 tcttctaaat atgaaaattc acgctcattt acttcttcac catcaccaag gtcaatccta
     3541 taatgctcga caacttcgcc atctacatag tggtcaaaat agaatttgaa atatccgata
     3601 taatcatcag tcatttctcc aaattcattt tctccaagag tttgattatg gtctttaaca
     3661 tcaatatctt tttgtcttag gacattaatt aattctttgg ttactatctg cccactataa
     3721 tcgggaacta attcgtggtt ttcattaaat tctacaatcc agtagtcttt tcttttttca
     3781 atattgttgc catcaaaaaa agaagcttca tcagcttcct tttcttggct tatttcattt
     3841 tctaagcttc cacgtattcc ttgattgcca ttctcttcac tgttgccatc aagttgttca
     3901 tctgtccttg gtattcctct ggattctctc tcatcatctt ctctgtcaat ccttgtgctc
     3961 tcatcatctt gactttctct ctcttgataa agtccactgc ctgatcttga atttccttca
     4021 gatggttcaa aagtgttttc tcctcgtaca tttcctgaag tttcttccag ttctccatgt
     4081 cctcgctccc tactaggtaa gacagtctta ggactgcgta agtcggattc ggaaatctct
     4141 ccataaattc cagatccttc tccatcatat ccaaggcttt ctcttcgatc ttcatcgcta
     4201 tttcctctgt cgcttccatc tgtgaaaact cgtccatctc tacttcttga cctatcatct
     4261 cgtctatata aatcatcttg acctcctcta tcttcgttta tattttctat atctttatta
     4321 tagtcggcag cgtccctttc tgtcagcagc ctagcacgat ctatttcctt agatttttct
     4381 attgtgctat ctataatatc ttcactaacc cttgatatta caagacctat ctgctctaaa
     4441 gaaattgtat taatcctaga aaaattattt tttaaatttt ccatatccat agggtagact
     4501 gtattaaacc tattagctac tgcataggat attgagtccc tcataaactt ttcaaagcta
     4561 agtctatcct ctatatctat tctcaaatca gcaagagcca agttaatgta ttcatctcca
     4621 taaattcggc ttaaagaaaa tatattttca ttaagtgaat cactagcctc aaatgaagaa
     4681 ttatttatta tttcttttaa agcctcgtta tggttttcgt gatcaaactg ccataagcta
     4741 acctcattaa tattcctatc cattgatgtc gtctgactaa tatcaaagat gtatgaaact
     4801 ctttgattta tatcacttcc aactaaaatt ggaataccct tctctcctct cataacaact
     4861 cgaccaaaat atttcttcca catatcaaaa gtcgcacaag ctctagcttc aggttctttg
     4921 ttatatatac ttaattggct agaaaaatca tatttctgat tattgcctat aacttttaag
     4981 agctttaaat attctttttc atcttgtaaa acctcatact ttattgcttc ttgtatattc
     5041 ttaaaatcat ttacttgcat ttcctacctc cattctatga atttctttta taatagccat
     5101 taaaacatca acaacaatag aattgccagc ctgcttatat agtttgcttg aggtgcaatt
     5161 ttttcttctt ggatgtactt tctctaattt gtctatatca ctgtcatcaa aacccatgag
     5221 tcttaaacat tctctttctg ttaaatacct atactgacca tttccaatat ctattatccc
     5281 agaattgggt actctcatct gttttgtaga aatagtataa gaaaaatctt taatcacttt
     5341 tagtcgccct ttaaaactat tatctatgcc tttatttaaa aatttgagca tataaggttg
     5401 ggtcattgtg taaagttcac ttacatcctt ttctaaaaat tcacttagtg gtcttgtttc
     5461 ttttctctcc aatttattaa aagaaaagtt atttgctcca agacatgaaa caacaaatat
     5521 cctttctctt ttttgaggta tcccaaagtc cattgcattt aagatttcat atttgctttc
     5581 atatccaaga tcttctagct cttttagata aataaaaaag gagtccctca tatttctatc
     5641 aaggactccc tttacattct cccaaattat ccatttaggt ttttctttca tttctttaat
     5701 tattctaatt gtttcaaata gtaagcttga tctagttcct gaattcttaa ctcctccctg
     5761 cttttttcct attcttgaaa agtcttggca aggacttcca tgcataatta agtctatctt
     5821 ttcattaggt attttatatc ctactactga ttttggctta taatcttctc cataaagtgc
     5881 gttgtatgac ttaacacaag ccttatctat ttctacataa tcaactactt cataaggtat
     5941 ctttaaatta ataaaagcct ttctaatagc acctatgcca ccgaaaagct ctaatatttt
     6001 taccttattc atttagatta ttcttgtacc tatctacaag ctcccttata ctatcttcga
     6061 tctctcttaa aaagtcttct ttatcaactt caccatagct aatctttgca agtttcattt
     6121 cccattcaga tgttgtttct gctgatttaa acttatcctc tacaatcgat acaagtctat
     6181 tgcctttttc tgttacaagt agatttttct tatctcttct tatgagatcc ttatggataa
     6241 gattttcgat aattcctgct cttgttgctg gcgttcctaa gccttttctc tccacttcta
     6301 tgtctttatc caaggtttct actcctgcat tttccatagc ttttaaaagt gtatcttcat
     6361 tataatgact tggagctttt gtaaatttct cagatatatt tttagaagtt agcttaattt
     6421 tatctccagt ctttatatct ggtaattcat tttcttgtct ttcctttaca tagctcttta
     6481 gatactttgt ataaccttca tctgttacag tcttgccact tgcattgaag ttaaacttat
     6541 catattcata tctggttttt ctgctagatt cctttaagtt atccgaacat gaagcaagta
     6601 atttatcctt aattagatta tagattttac tttctttatc agataaatct ttagcttttc
     6661 caatccctga tatagtagga ataattgcat agtggtctgt aaccttagac gaattaaaaa
     6721 tagacttaaa atttgattca ttgattttaa agtcatcttc aagtccttct aataattctt
     6781 tcatagtatt aaccatatca tcggttaaat acctactatc tgttcttggg tatgttatta
     6841 gctttttttc atacagacct tgtgccagat ttaaagtgtc atttgctgaa tatccaaaat
     6901 atttatttgc ttctctttgt aaggtagtga gatcataagg cttatctggt tttgtgctta
     6961 tatctttatc ttctacctct gtaataacta tttcatcttc taacaagttt aatagttgtt
     7021 ctgcaacttc aattttatca attctatctg atacaagttt caatcctcca taagataggt
     7081 caactgtata atatttttgc ttcttaaata ggtttatttc actatccctc ttagctatta
     7141 aatataaagt tggtgtttgt actctaccaa cagaatatgt ttccttgtaa atgcaagaat
     7201 aaagcctact taaattcatt cctaccagcc aatccgcaat tgctcttgca cttgctgatc
     7261 tatataagtc ttcaaagttt tctccgtctt taagatttct aaagccatct tcaatagcct
     7321 tgttttccat tgaagatatc caaagtcttt gaattttctt cttacattta gcttgattat
     7381 atacaagcct aaaaataagc tctccctctc ttccagcatc acaagcattt attactgtgt
     7441 cgatgttctt atcgtttaaa agcttcttta aaactccata ttgcttttta gtgcttttag
     7501 atacttcata aatatattct tctggaatta taggaagagt gtctatcgtc catttcttcc
     7561 atttttcatc gtgtctttct ggacttgcca tttgaattaa atgacctaca caccaagaaa
     7621 caatgtatcc attcccctca taatatccgt tttttcttgt tcttgctcca attacttttg
     7681 caattgtaac tgctacgctt ggcttttctg ctattactaa cttcattaat acctccataa
     7741 ataaaaaaaa gagcgaaaga tttctctctc gctcaaaata tttctcccga atattgtatt
     7801 ttttcgggag aattctatta cttattaatt tctttttcta gttctttaag aacttttata
     7861 atttcatcaa attttggata ttctccatat atcatttctt ccatagcctt ataatctttt
     7921 tctatttcag aaaatcggta ttttcttggg actaatttta atctaccatc taatgcttct
     7981 tcataccttg cccacttcct aggataaaac ttcattttga attcagttac tttctttaat
     8041 aactccttat cctctaaggc tctgtctttg aaatctgaat ttgctatttt atataagtca
     8101 taaaagtgtc ttgcatacct atgtggcata ggagaagatt ctggtctatt tgcttcgtgg
     8161 tgtaaaattg ttgctttttc ccaaaaagtt ctctctgctg atactgttct tatattagtt
     8221 ttttctttaa aaacatttgg ataggcttct ccaattattg gcaggatttc cacttcaatt
     8281 gcaggtgtcc atgctgcaag acttcctatt tcaagcctta tattttgtgt caaataatta
     8341 gattcaaaga ttttaggata tttacataga attgtttgtg gatcaagagt atcaatcata
     8401 aattcaaagt ccatgtcctt gaaatcttgt tctaagactt ttaataattc atctcttaaa
     8461 aagatttcag tcttttcatt gacttctttg ttgaatttat cctgtttagt atttgacctt
     8521 tctaaccaag gttctttttc ttcgtaacct aatactcgcc aatctaagat tagatctata
     8581 tcttcagaaa atctttctat aagtccaaag cattttgaaa gtgatgttcc tcctttaaag
     8641 gtaaaatatt ccctccattt gttttcatta aatagatagt ctaagataaa agtaacccaa
     8701 tagtcttttt caatgacggc ttcagatata tttttctttc ttgcagtatt gactattaaa
     8761 actcttaaat cttctttgtt ttctatataa aacttactca tttttgccct cacaaatttc
     8821 ccttattact tcatatatcc atgctggcac tgattttgat tcgttcatta agtctattct
     8881 ttctttttca cttaagtttt ctcttatttt ttgtataact tgggcattga tattttcttt
     8941 gcccaaagac ttaattgctt gaataactgt tgcggtcttt agggacatat tggcaatttc
     9001 tcctggattt acttttttaa attctaaaac cgtatctcca attttatatt ccttatatct
     9061 tccactagat atatatttat agtgagttgg aacttgtgtt gaaagtccta ataagtttaa
     9121 ggctgtgcta ttatatggtg caatattcca attgtatttt cttgctatag caagtgccaa
     9181 ctcgtgaatt gatactgctt cgtactctcc aattaattca ctgtatcttg gattataata
     9241 gaatccatta attactcttg ttattttatt ttcactaact agttgattta aa
//
LOCUS       DAUCLR010000073         9243 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.073, whole genome shotgun sequence.
ACCESSION   DAUCLR010000073 DAUCLR010000000
VERSION     DAUCLR010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9243
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.073"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..773
                     /locus_tag="VGT87_001447"
     CDS             <1..773
                     /locus_tag="VGT87_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEQ3814285.1"
                     /translation="KEMIMEAFQQVFPEVKGAYEKCRFKGPDTETAHLYGELAQETFS
                     ILENGIAYQVFLNEGLMTGIFLDQHDVRRALVDGLAMGKSLLNLFSYTAAFSVAAAMG
                     GAIETTSVDLAKRSRELSLAHFEHNQLNLASHHFVVMDVFEYFKYAKRKKLIFDVIVI
                     DPPSFARNKKQTFSVSRDYHKLITEALDILSPKGTIIASTNAANMTVSQFKKQIIKGF
                     GSRRPESMTLQQLPSDFTINKADERSNYLKVFTIKVRE"
     gene            770..1456
                     /locus_tag="VGT87_001448"
     CDS             770..1456
                     /locus_tag="VGT87_001448"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEQ3814286.1"
                     /translation="MRIVAPVMPRHFDEAQAIDISKYEDVNLIEWRADFLPKDEIVAV
                     APAIFEKFAGKEIIFTLRTVQEGGNITLSSQEYVDIIKEINAIYNPDYIDFEYFTHKS
                     VFQEMLDFPNLILSYHNFEETPENLMEAFSEMTKLAPRVVKIAVMPQSEQDVLDLMNY
                     TRGFKTLNPEQEFATISMGKLGRLSRFAGDVIGSSWTYVSLDHVSGPGQVTLNDMKRI
                     IEVLEMDISN"
     gene            1550..2716
                     /gene="aroC"
                     /locus_tag="VGT87_001449"
     CDS             1550..2716
                     /gene="aroC"
                     /locus_tag="VGT87_001449"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEQ3814287.1"
                     /translation="MRYLTAGESHGPSLTAIIEGIPAGLTLHPADIDHELQRRQGGYG
                     RGARMSIETDRVQISSGVRHGKTTGAPITLTVINKDHQKWLDVMAVGDIEETLKLKRR
                     VKHPRPGHADLVGGIKYHFNDLRDALERSSARETTMRVAVGAVAKRILAELGIDMLHH
                     ILIFGGITITIPSKLSFRELQERALHSELSIVNPKQEEEIKTYIDKIKKEGDTIGGII
                     ETIVQGVPAGLGSYVQWDKKLDAKLAQAVLSINAFKGVEFGVGFDMGFQKGSQVMDEI
                     TWTPTQGYGRQTNHLGGFEGGMTTGQPLVVKGVMKPIPTLYKPLMSVDIDSHEPYKAT
                     VERSDPTALPAAGVIMENVVATVLAKEILETFSSTTMSELQKAFSDYRAYVKQF"
     gene            2777..3118
                     /locus_tag="VGT87_001450"
     CDS             2777..3118
                     /locus_tag="VGT87_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEQ3814288.1"
                     /translation="MSQEIYDYANQLERAVRALPEYQKVLEVKEAIQADASASELFDE
                     FVAMQEKIQGMMQSGQMPTAEEQTSIQELSQKIEANDQLKAYFEAQQALSVYMSDIER
                     IVFAPLKDLVK"
     gene            3340..4692
                     /gene="gorA"
                     /locus_tag="VGT87_001451"
     CDS             3340..4692
                     /gene="gorA"
                     /locus_tag="VGT87_001451"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEQ3814289.1"
                     /translation="MVIPYDYIVIGGGSAGIASANRAAMHEAKVLLAEGKEIGGTCVN
                     LGCVPKKVMWYGAQVADILGTYAKDYGFDFKDKAFDFKQLKANRQAYIDRIHASYERG
                     FEQNGVDRIYDYAVFKDAHTVEIAGQLYTAPHILIATGGHPVFPDIEGAQYGISSDGF
                     FALDEVPKRTAVVGAGYIAVELAGVLHALGSKTDLFIRHDRPLRSFDKAIVDVLVEEM
                     AVGGPCLHPHSEVAKVVKNTDESLTLYLKDGQEVEVDQLIWAIGRKPNLEGFGLDKTG
                     VTLNDKGYIETDAYENTSVKGIYAVGDVNGKLALTPVAVAAGRRLSEHLFNNKTDEKL
                     DYQNVATVIFSHPVIGSVGLSEEAAVKKYGQEAVKTYQSRFTSMFTAITNHRQPCLMK
                     LVTVGDTEKIVGLHGIGYGVDEMIQGFAVAIKMGATKADFDNTVAIHPTGSEEFVTMR
                     "
     gene            complement(4772..6049)
                     /locus_tag="VGT87_001452"
     CDS             complement(4772..6049)
                     /locus_tag="VGT87_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEQ3814290.1"
                     /translation="MTTYDSISWIHTFKANGRKTDLARMAWLLEALGRPQDKFPAIHV
                     VGTNGKGSVTAYLQHILSTSGYQVGTFTSPFIISFHDRICLNGQPISDLELAQCVSVI
                     RPILTKMSLETNWDRPTEFELVTLIMFCYFANLKPVDIAIIEAGIGGKTDATNVFHAL
                     AVVCPSISFDHQERLGYSLAEIAHQKAEVIKAKEPVIIGQLEQEASQVFQQVTQKAGS
                     RLYQLGKDFLLNPSEKTFSFQHDNLNLTKLRLKLLGQHQKANACLAIMTAQLLRKSFP
                     KISPKTIQKGIEATTWPGRSEFIQPNLLLDGAHNPDSIAKLKSLLQEEFPKRPIHILF
                     AGLKRKPLADLLAQLDTFEVSVTTFDFPEANLLEDYPDKYPQVKDYRDWLQGSVTSDE
                     LFVVTGSLYFISEVRQYCLSNANIFKNIFPLIT"
     gene            complement(6079..6519)
                     /locus_tag="VGT87_001453"
     CDS             complement(6079..6519)
                     /locus_tag="VGT87_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814291.1"
                     /translation="MSSHYSRQQKPRKSGGVPTEHIKTGFTAFQKSIALIGSILSIIV
                     ATITITRALQPVPDKTSNDTPKESSNTIVKIIEKESSQGNQNHTGTSETTDDNKTTLP
                     SSSTTPSHNETTPTAPETPSTTEGTQLPNATGAEDANTGLPTNP"
     gene            6754..7896
                     /locus_tag="VGT87_001454"
     CDS             6754..7896
                     /locus_tag="VGT87_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEQ3814292.1"
                     /translation="MIYFDNAATTIPYGEALKTYQEVATKIYGNPSSLHQLGTNASRI
                     LEASRKQIAGLLGVKSEEIFFTSGGTESANWAIKGIAFEKNAFGKHIIISAIEHPAVS
                     ESVKWLLTQGFEVSYAPVTTQGVVDVNALAELIRPDTILISIMAVNNEMGAIQPIRAI
                     SNLLANQPTITFHVDAVQAIGKIPLCDYMTNRVDLASFSGHKFHSVRGVGFLYKKAGK
                     RLNPLLSGGGQEQELRSTTENVAGIASMAKALRIVTEKQVSVLPKLTAMRDVIYKALS
                     AYPDVTVFSAQEGFAPNILTFGIRGVRGEVIVHAFEKHEIYISTTSACSSKAGKPAGS
                     LVAMGIPVKTAQTAVRISLDDDNDMGQVEQFLTIFKQIYDKTQKVR"
     gene            7908..9122
                     /gene="thiI"
                     /locus_tag="VGT87_001455"
     CDS             7908..9122
                     /gene="thiI"
                     /locus_tag="VGT87_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEQ3814293.1"
                     /translation="MDYSEIMVRHGELSTKGKNRMRFINKLKNNIQDVLAPFPAITVR
                     SDRDRTHVSLNGTDYQPIVEALKLVFGVQALSPVYKLEKSVPLLVTAVQDIMTSLYRD
                     GLTFKIATKRSDHAFELDSRELNSLLGGAVFEVLPNIQAQMKHPDVTLKVEIRDEAAY
                     ISYEEIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGLDIEVVHFASPPYTSPG
                     ALAKAQDLTRRLTRFGGNIQFIEVPFTEIQEEIKNKAPEAYLMTLTRRFMMRITDAIR
                     EQRKGLVIVNGESLGQVASQTLESMQAINAVTSTPIIRPVVTMDKLEIIEMAQAIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKLGNAEKYEERFDIDGLVQRAVSGIVVTEITPEIV
                     NDEVENLIDALL"
BASE COUNT         2786 a         1642 c         2078 g         2737 t
ORIGIN      
        1 aaaaagaaat gattatggag gcttttcaac aggtttttcc tgaagtcaaa ggagcttatg
       61 aaaagtgtcg ctttaaaggt ccagatactg aaactgctca tctgtatggt gagctggcac
      121 aagaaacctt ttcgatttta gaaaatggca tagcctatca agtcttcttg aatgagggct
      181 taatgacagg tatttttcta gaccagcatg atgttagaag agcactagta gatggtttag
      241 ctatgggcaa gtcccttctt aacctatttt cctatacggc tgccttttct gtagcagctg
      301 ctatgggtgg tgctatcgag accacttctg tggacttggc taaacgatcg agagaattat
      361 cactagctca ttttgagcat aaccagttaa acttagcctc acatcatttt gtggtgatgg
      421 atgtttttga atacttcaaa tatgccaaac ggaaaaaatt aatctttgat gtgattgtga
      481 ttgacccacc aagctttgct agaaataaaa aacaaacttt ctctgtgagc agggactacc
      541 acaaattaat cacagaggcc ttggacatat tgagtcctaa gggaactatt atagcttcaa
      601 caaatgctgc taatatgact gttagccagt tcaaaaagca aattatcaaa ggatttggaa
      661 gtcgtcgtcc tgagtctatg actctccagc agcttccgag tgactttact atcaataaag
      721 cagatgaaag aagtaactat ttaaaggtat ttactataaa ggtacgagaa tgagaattgt
      781 agcaccagtc atgccaagac attttgacga ggctcaagcc attgatattt ctaaatatga
      841 agatgttaat ttaattgaat ggcgagcaga ctttcttccc aaggatgaga ttgtcgcagt
      901 agcaccagct atttttgaaa aatttgccgg aaaagaaatt atctttaccc ttcggaccgt
      961 tcaagaaggg gggaatatta ccctttcaag tcaggagtat gttgatatta tcaaagaaat
     1021 caatgctatt tataatccag actatattga ctttgagtat tttacgcaca agtcagtatt
     1081 tcaagaaatg cttgattttc caaatcttat tttgtcttat cacaactttg aagagactcc
     1141 tgaaaattta atggaagctt tttcagaaat gaccaagttg gcaccgcgtg tggttaaaat
     1201 tgctgtgatg ccccagagtg aacaagatgt tctagatttg atgaactata ccagaggctt
     1261 taagacgctc aatcctgagc aagaatttgc aactatatct atggggaaat taggaagatt
     1321 gtctcgtttt gcgggagatg tcattggctc ttcctggaca tacgtttcat tagatcatgt
     1381 tagcggtcca ggtcaagtga ctctcaatga tatgaaacgt attattgagg tcttagaaat
     1441 ggatatctct aactagtgaa tggtctaaaa atattatttc tacaatagtt aagaaatgat
     1501 gtttttagat taatgttttt aaaataagtg gaaccaaaaa ggagaaaaat tgcgttattt
     1561 aacagcagga gaatctcatg ggccatcctt aacagctatt attgaaggta taccagcagg
     1621 attaactctt catccagcgg atattgatca cgaattacag cgacgtcaag gaggttatgg
     1681 ccgtggtgcc agaatgtcta ttgagactga tcgggttcaa atatcctcag gagttcgtca
     1741 tggcaaaaca acaggggccc ccattacctt aacggtaatt aataaggacc accaaaaatg
     1801 gctagatgtc atggctgtag gagacattga agagactctc aagttaaagc gtcgtgttaa
     1861 gcatcctaga ccaggacatg cggatttggt aggaggtatt aaatatcatt ttaacgattt
     1921 aagagatgct ttagagcgtt cctcggctag agaaacaacg atgagagtgg ctgtaggcgc
     1981 tgttgctaaa cgtatcttag ctgaacttgg aattgatatg ttacatcata tcttaatatt
     2041 tggtggtata acgatcacaa taccttctaa gctctctttt agagagttgc aagaaagggc
     2101 tctccattca gaattatcta ttgttaatcc aaagcaagaa gaagaaatta aaacgtatat
     2161 tgataagata aaaaaagaag gggatactat tggtggtatt atcgaaacga tagttcaagg
     2221 ggtgccagca ggtcttggat cttatgtcca atgggataaa aaattagatg ccaagctggc
     2281 tcaagcagtt ctttccatta atgcttttaa aggggtggaa tttggagttg ggtttgatat
     2341 gggattccaa aagggctctc aggtaatgga tgaaatcacc tggacaccta cccaaggcta
     2401 tggtcgtcaa acaaaccact taggtggctt tgaaggtggc atgaccacag gacaacctct
     2461 tgttgttaaa ggggttatga agcccattcc tactttgtat aaaccactca tgtcggtaga
     2521 tattgatagt catgaacctt ataaagcaac ggtggaacgc tctgatccga cggccttacc
     2581 agctgcaggg gttatcatgg aaaatgtggt ggcgacggtg cttgccaaag agattttaga
     2641 gactttttct tctactacca tgtctgaatt gcaaaaagct ttttcggact atcgtgccta
     2701 cgtcaagcaa ttttagatag cgagatactg gcttttatgg tatcatacag gtattaataa
     2761 acaaaaggag actgtcatgt cacaagaaat ttatgactat gctaatcaac ttgaaagagc
     2821 agttcgtgct ctgccagaat accaaaaagt gttagaagta aaagaggcca ttcaagcaga
     2881 cgctagtgct agcgaactgt ttgatgagtt tgtcgctatg caagaaaaaa tccaagggat
     2941 gatgcaatca ggccaaatgc caacggctga agaacaaaca agcattcaag aacttagcca
     3001 aaagattgaa gctaatgacc agctaaaagc ttattttgaa gcgcagcagg ccttatctgt
     3061 ttacatgagt gatattgagc gtattgtatt tgctccgttg aaagatttag tcaagtaagc
     3121 caattaagat aggaaaaaaa acagattgct ggcagctcgt caggatctgt tttttagtgt
     3181 ttattttctt tgtatcagaa agttgagcct ctttcttatt tgtgatatag tcaaataaat
     3241 tgaccaaaat agcaggccag tagtggcttt aatgaaggca agttgtctgc taactggcta
     3301 tgctaaaaca tcagtattag aaagtaaaag gttataaaaa tggttattcc ttatgattat
     3361 attgttattg gtggagggag tgcaggtatt gcttctgcga acagggcagc catgcatgag
     3421 gccaaagtat tgttagctga aggtaaagag attgggggaa cctgtgttaa cctaggctgc
     3481 gttcctaaaa aagtcatgtg gtatggcgct caagtagcag atattttagg gacttacgct
     3541 aaggattatg gatttgactt taaagacaaa gcatttgatt ttaaacaatt gaaagcaaat
     3601 cgtcaagctt atattgatcg gattcatgct tcttatgagc gaggctttga gcagaatggt
     3661 gttgaccgta tttatgatta tgctgttttc aaagatgctc atacggtaga aattgcaggg
     3721 cagctttata ccgcaccgca tattttgatt gccacaggag gacatcctgt ttttccagat
     3781 attgaggggg cacaatacgg tatcagctcg gacggctttt ttgccttaga tgaggtacca
     3841 aaacgtacgg ctgtggttgg ggcaggctat attgctgtgg aacttgccgg tgttttgcat
     3901 gctttggggt cgaagacgga tttgtttatc cgtcacgatc gacctttaag aagttttgac
     3961 aaagccattg tggatgtttt agttgaggaa atggcagttg gtggtccttg cttacaccct
     4021 cattcagaag tggcaaaggt tgtcaaaaat actgatgagt ccttgaccct ttacctcaaa
     4081 gatggccaag aagttgaggt tgaccagctc atttgggcta tcggtcgcaa gcctaatcta
     4141 gaaggttttg gtcttgacaa aactggagtg acgttaaatg ataaaggtta tattgagaca
     4201 gatgcctatg aaaacacgtc tgtcaaaggt atttatgccg tgggagatgt taatgggaaa
     4261 ctggctttaa ctccagttgc tgtggcagct ggacgtcgcc tatcagagca tcttttcaat
     4321 aacaaaacag acgagaaatt ggactaccaa aacgttgcta ctgtgatttt tagtcaccct
     4381 gttattggct ctgttggctt gtcagaagaa gcagcagtca aaaagtatgg gcaagaagca
     4441 gttaaaacat atcaatcacg ttttacgtct atgtttactg ctatcactaa ccatcgccaa
     4501 ccttgtctga tgaagttggt aacagtcgga gataccgaaa aaatagttgg tcttcacgga
     4561 atcggctatg gggttgacga aatgattcag ggctttgcag tagctatcaa aatgggagca
     4621 accaaggcgg attttgataa caccgttgct attcacccaa ctggctcaga agaatttgtc
     4681 accatgcgct aaaaaatgta gcctaaggca ataaaaaatg atgttaacag ttttcgagga
     4741 aagtcttcta aactgctagc atcgtttttt gttaggtgat tagggggaat atgtttttaa
     4801 agatatttgc gttgctcaga caatattgcc ttacctcgga gataaaataa agtgaccctg
     4861 taacaacaaa gagttcatca gatgttacgg aaccttgtaa ccagtctctg taatctttta
     4921 cctgtgggta tttatcagga taatcctcta aaagatttgc ttcagggaaa tcaaaagtcg
     4981 tgacagatac ttcaaaagta tctagttgag ccagcaagtc ggctaaaggc ttacgtttga
     5041 gacctgcaaa gaggatatga ataggacgtt tgggaaattc ttcttgcagt aaagacttta
     5101 atttagcaat ggaatctgga ttgtgagcgc cgtctaatag aagattaggt tgaataaatt
     5161 cggaccggcc aggccaagtt gttgcctcaa ttcctttttg aatcgttttt ggagatattt
     5221 taggaaagga ttttctaagt aattgagctg tcataatggc taaacaggca ttcgcttttt
     5281 gatgttgacc taatagtttg agacggagtt tggttaaatt caagttatcg tgttgaaagg
     5341 aaaacgtctt ttctgaagga ttgagcaaaa aatcttttcc taattgatat aagcgtgaac
     5401 cggctttttg ggtaacttgt tgaaagactt gactagcctc ctgctcaagt tgaccgataa
     5461 taacgggttc tttagctttg ataacttctg ctttttggtg agcaatttct gctaggctat
     5521 aacctaaacg ttcttggtga tcaaaactaa tggaagggca tacgacggcc agagcatgaa
     5581 aaacattggt tgcgtcggtt ttcccaccaa tccctgcctc aataatggca atatcaacag
     5641 gtttaagatt ggcaaagtag caaaacatta tgagagtcac taactcaaac tcggtaggtc
     5701 tatcccaatt tgtttcaagg gacattttag tgagaatggg tctaatcacg ctcacacatt
     5761 gcgccaattc taggtctgaa atgggctggc cattaaggca gatccgatcg tgaaaagaga
     5821 tgataaaagg agatgtaaag gttccgacct gataaccaga agttgacagg atatgctgta
     5881 agtaggctgt cacagagccc ttgccgttag ttccaacaac gtgaatagca gggaatttgt
     5941 cttgtggccg gcctagtgcc tcaagtaacc aagccattct ggccaaatcg gtcttgcgac
     6001 cattagcctt aaaggtatga atccaggaaa tgctatcata ggtagtcatt actatttctt
     6061 gtccttctaa caatgcttct aagggttcgt aggaagacca gtattagcat cttcagctcc
     6121 agttgcatta gggagttgcg tcccttcagt ggttgatggt gtctcagggg ctgtaggagt
     6181 tgtctcgttg tgagaaggtg tggtagaaga tgacggaaga gttgtcttat tatcatctgt
     6241 ggtttctgaa gtacctgtat ggttttgatt accctgagaa gactcttttt caataatctt
     6301 gacaatggta ttactcgatt ctttgggtgt atcattagag gtcttatctg gtacaggctg
     6361 cagtgctctt gtaatggtaa tggttgccac aatgatactc aaaatactac caataagtgc
     6421 aatggatttt tggaaagctg taaaacctgt tttaatatgc tcggtaggga cacctcctga
     6481 tttacgaggt ttttgttgac gggaataatg tgatgacata tttttctcct ttaaaatcat
     6541 cgtaatgcca agattaagga ggaggaaata gaagtcctag ctcctcttta acacttcatc
     6601 tatcagtata atctagaaaa aaagtgaagt caacggttta ccttatgtct aggacaagga
     6661 agtcctttgg gcttattgta atacgaatat tcttgatttc attttagcta ttttgtgata
     6721 gaataaagta ttgagtaaaa atgaaggtga cacatgattt attttgataa tgcagccaca
     6781 accatacctt atggagaggc tcttaaaact taccaagaag tagcaacaaa aatatatggc
     6841 aatccctcaa gtctgcacca attagggaca aatgcgtctc gcattttaga agcttcccgt
     6901 aaacagattg ctggtttgtt gggtgttaag tcagaagaaa tcttttttac gtctggtggg
     6961 actgaaagtg ctaattgggc tattaaggga attgcttttg aaaaaaacgc ttttggcaaa
     7021 catattatta tctcagccat tgaacaccct gccgtgagtg aaagtgtcaa atggcttctc
     7081 acacaaggat tcgaagtttc ttatgctcca gtgacaacac agggagttgt tgatgtgaat
     7141 gctttagctg agctgattag accagacacc atcttaatct cgattatggc tgttaataat
     7201 gaaatgggag ctatccaacc gattagagcc atttcaaacc tattggctaa ccaaccaacg
     7261 atcacctttc acgttgatgc tgttcaagcc attggtaaaa taccactctg tgattatatg
     7321 actaatcgtg tggatttggc ttctttttca gggcacaaat ttcatagtgt tcgtggtgtt
     7381 ggcttccttt ataaaaaagc aggcaagcga ctcaatcctc tcttgtcagg cggcggccaa
     7441 gagcaggagt tacgttcaac cactgaaaat gttgcaggaa ttgctagcat ggccaaagca
     7501 ttaagaatag tcactgaaaa acaagtgagc gtcttaccta aactaacagc gatgcgagat
     7561 gtgatttaca aagctttgtc cgcttaccca gatgtgacag tattttcagc tcaagaaggc
     7621 tttgcgccca atattttgac ctttgggatt agaggggttc gaggagaagt tattgtccac
     7681 gcctttgaaa aacatgagat ttatatttcg accacaagtg catgttcgtc caaagcaggt
     7741 aagccagcag gaagcctagt ggctatgggg attcctgtaa aaactgccca gacagccgtt
     7801 cgtattagtc tagacgatga caacgatatg ggacaagtag agcagttttt gaccatattc
     7861 aaacagattt atgataaaac acagaaagtt aggtaagatt aggtaagatg gactattcag
     7921 aaattatggt tcgccatggt gaactatcaa ctaaagggaa gaatcgcatg cgattcatta
     7981 acaaacttaa aaacaatatt caagacgtac tggctccttt cccagccatt actgtccgtt
     8041 cagatcgtga taggacccat gtgtctctaa atggcacaga ttatcagcct attgtagaag
     8101 ctttaaagct ggtgtttggt gttcaagctc tatctcctgt ttataaactt gaaaaaagtg
     8161 tccctctttt ggtaacagct gttcaagata tcatgacttc tctttatcgt gatggactga
     8221 cgtttaagat tgccactaag cgtagtgatc acgcgtttga attggatagt cgtgagctta
     8281 acagccttct tggtggagct gtttttgagg ttttacccaa tatccaagcc cagatgaaac
     8341 atcccgatgt gactctcaaa gtcgaaattc gtgatgaagc agcttacatt tcttatgaag
     8401 agattaaggg agcaggcggg ttaccagtag gaacgtcagg gaaagggatg ctgatgttat
     8461 cgggcggtat tgattcacca gtagctgggt atttagccct taaacgtggc cttgatattg
     8521 aagttgttca ttttgctagc cctccgtaca ccagtccagg tgccttggct aaagcccaag
     8581 accttactcg tcggttaact cgttttggtg gaaatatcca atttatcgag gttcctttta
     8641 cagaaattca agaagaaatt aaaaataagg caccagaggc ttaccttatg acattaaccc
     8701 gccgctttat gatgcgcatc acagatgcca ttcgtgagca acgtaagggt cttgttattg
     8761 ttaacggtga gagcctaggt caggttgcta gtcaaaccct tgaaagcatg caggccatta
     8821 atgcggtaac ctcaacccca attattagac cagtcgtgac aatggataaa cttgaaatta
     8881 tcgagatggc tcaggcgatt gatacttttg acatttctat tcaaccgttc gaagattgct
     8941 gtactatttt tgcaccagac cgtcctaaaa ccaaccctaa actaggtaat gctgaaaaat
     9001 atgaagaacg ctttgatatt gatggtcttg tccagcgtgc tgtgtcagga attgttgtca
     9061 cagaaatcac acctgaaatc gtcaatgatg aggttgaaaa tctcattgat gctttacttt
     9121 aaaagaaaag atcaagtgta aatcttttag tagtcagtaa tgatactatt gaaaagcctc
     9181 tataagggct tttttgctgc aatgaaggag gaaaattttg aggcaagatg ttataataga
     9241 gaa
//
LOCUS       DAUCLR010000074         9058 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.074, whole genome shotgun sequence.
ACCESSION   DAUCLR010000074 DAUCLR010000000
VERSION     DAUCLR010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9058
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.074"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..647
                     /locus_tag="VGT87_001456"
     CDS             <1..647
                     /locus_tag="VGT87_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814294.1"
                     /translation="FDYENRRLNYFMKSEENMRVEFSNYLHDDVLQNIIAIKNLLSLE
                     NSNITHGFIVNELNDLVSGIRDEMDTYHPIVSANQTMKENIQSLFDDIVKSRKSNTLL
                     YFNCSDNMVVPSPYGDIVYRFIKELINNAIKYGDGKDIRLSLTIQSDIIIIEESNQVV
                     EKVHSINYGRGLKSFQETLAAFDGDLELQMDTKQFTIRILLPIDWKLCYEDFIN"
     gene            628..1233
                     /locus_tag="VGT87_001457"
     CDS             628..1233
                     /locus_tag="VGT87_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEQ3814295.1"
                     /translation="MKILLIDDHRLFAKSIQLLFQQYDEVDVIDTITSHFNDVTIDLS
                     KYDIILLDINLTNISKENGLEIAKELIQSTPHLKVVMLTGYVKSIYRERAKKVGAYGF
                     VDKNIDPKQLISILKKVDSGKKYFEQIESQDYVESLTDQEIAILNLSKKGFSIKEIEE
                     TLQISRRTVFNHLTHIYSKLLVNNKQEAIYKAEQLGYFMDF"
     gene            complement(1786..3366)
                     /locus_tag="VGT87_001458"
     CDS             complement(1786..3366)
                     /locus_tag="VGT87_001458"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEQ3814296.1"
                     /translation="MAEKTTSLRQALWHSADQLRGQMDANDYKNYLLGLIFYKHLSDK
                     LLFAVCDNLDQHFNTFTEAQKIFEDAYQDEDLKDDLISVVTGDLGYFIEPTLTFEKLI
                     QDVYHNTFQLESLAQGFRDIEQSGEDFENLFEDIDLYSKKLGSTPQKQNQTIANVMKT
                     LNEIDFEAVDGDTLGDAYEYLIGEFASESGKKAGEFYTPQAVSHLMTQIVFLGREDQK
                     GMTLYDPAMGSGSLLLNAKKYSNQSDTVSYYGQEINTSTYNLARMNMMLHGVAIENQH
                     LSNADTLDADWPTDEPTNFDGVLMNPPYSLKWSATAGFLTDPRFSSYGVLAPKSKADF
                     AFLLHGFYHLKNTGTMAIVLPHGVLFRGAAEGKIRQKLLEQGAIDTIIGLPSNIFYNT
                     SIPTTIIILKKNRTNKDVFFIDASKEFDKGKNQNTMTDNHIKKILDAYKSRDNSDKFS
                     YLASFDEIIENDYNLNIPRYVDTFEEVPVKPLPELAKQLSDIDQEIAKTNAKLDQLMK
                     QLVGTTKEAQDELDTFRN"
     gene            complement(3379..4554)
                     /locus_tag="VGT87_001459"
     CDS             complement(3379..4554)
                     /locus_tag="VGT87_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEQ3814297.1"
                     /translation="MTKSKQPQYRFDGFKGEWEEKELGDIVQITMGQSPSSQNYTTNP
                     SDYILVQGNADIKNGYVFPRVWTTQITKQADKGDIILSVRAPVGDVGKTNYHVIIGRG
                     VAAIKGNEFIFQILKYLKEIGYWKRISTGSTFDSISSSDIKYAKIQIPSLPEQEAIGE
                     LFQTVDQLIQLQDQKLATLKEQKQTFLRKMFPAQGQKVPEIRLQGFDGEWEEKKLGEI
                     SRMFSGGTPNVGIPEYYNGNIPFIRSAEINSDQTELSITDKGLSNSSAKLVEKNTLLY
                     ALYGATSGEVGLSRISGAINQAILAIIPEKKYSSLFIKNWLYKQKSSIIKKYLQGGQG
                     NLSGSIVKELTIHFPSLSEQEAIGDFFQTLDQQMSQTEDKLTELKALKQTLLNRLFV"
     gene            complement(4567..7545)
                     /locus_tag="VGT87_001460"
     CDS             complement(4567..7545)
                     /locus_tag="VGT87_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEQ3814298.1"
                     /translation="MGNQTKTELELEKELIHLLETGESQWTYRKELKTEDALWDNFFK
                     ILAQNNTQYLNEEPLTASEKEQIKNQLNFVNYYEAAKWLAGENGIAKVQVQREDAKLG
                     AIRLEVVKADNVAGGTSVYEIANQVAFSGSRDRRGDVTLLINGLPMIQIELKSQNHQC
                     IEAFNQVKKYDKEGQFRGIFSTLQMFVVSNKTDTRYIAAAKENKLNPNFLTQWVDQNN
                     KPQKDLFAFAKEVLSIPLAHQMVMTYSVIDDDKKALILLRPYQIHAIEAVAEASRHRK
                     SGYIWHTTGSGKTLTSYKVARNILQIPAVEKSIFVIDRKDLDNQTTSAFQSYAQNDIF
                     DVDETEDTRQLIKNLESSDRRVVVTTIQKLNAMISQMESYDTPKFKKLKERLAHLNVV
                     FVVDECHRAVTPERQRHLTNTFRNSCWYGFTGTPIFVENKRAQLGDLAQTTEQQYGKC
                     LHQYTVKEAIHDKAVLGFQVEYKTTIPDMPEDSIPEEAYDHEEHMLAVLNSIINQSRK
                     KLGFNNGIGQTFEGLLTVKSIARAQAYYDLIQKVKAGETDLVISKKVKEKLPDFPKVA
                     ITYSITENDNASISRQDKMTKNLEDYNHLFGTNFTIDNLQGYNRDLNDRLARKKDKFK
                     DRHEQLDLVIVVDRLLTGFDAPCLSTIFIDRQPMKPQHIIQAFSRTNRIFESRKHYGQ
                     VVTFQTPLRFKEAVDKALSLYSNGGENDVLAPSWEEEKARFLEKVTVLKNIVPDPDAF
                     PTIESAQTAFLKQYAKAYQAFDKLYASVQVYSDFNETLLSEVGLSDEVIDTYKGTYQN
                     VIAEIRKRRDDDEAIPEINIDYELESIQMDDINYHYILTLIQAFVDQEQEALQERLND
                     NPMDQYIQDLAKSNPAMADSLAELWQDIQKEPKAYEGKSIVYELDNLIGDKIQRAIKH
                     FADQWKADPDKLAFVATNYHSANSTKQVGMSTLKESLDYQAYKEKQGDSAMNKLKYKS
                     QFERELVQFIRDQIQPLKGH"
     gene            7826..8968
                     /locus_tag="VGT87_001461"
                     /pseudo
     CDS             7826..8968
                     /locus_tag="VGT87_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285157.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
BASE COUNT         2749 a         1538 c         1733 g         3038 t
ORIGIN      
        1 attttgacta tgaaaataga agactgaact attttatgaa atcagaggaa aacatgagag
       61 tggagttctc aaattatttg catgatgatg ttttacaaaa tatcatagcg ataaaaaact
      121 tgctttctct agaaaatagt aatataacac atggttttat tgttaatgag ttgaatgatt
      181 tagtttctgg tattagagat gaaatggata cttaccaccc aatagtttcc gctaatcaaa
      241 caatgaaaga aaacatacag tctctttttg atgatattgt taaaagtaga aaaagtaata
      301 ccctattgta ttttaactgt tcagataata tggtagtgcc atctccatat ggagatattg
      361 tttataggtt tataaaagag ctaataaaca atgctataaa atatggagat ggaaaagaca
      421 ttcgtttatc tttaacgatt caatctgata ttattatcat tgaggagagc aatcaagtag
      481 tggaaaaagt acactctata aattatggta gaggattgaa atcatttcaa gaaacactag
      541 cagcttttga tggagatcta gaactacaaa tggatactaa acaatttact atcagaatat
      601 tacttcccat agattggaaa ttgtgttatg aagattttat taattgatga ccacagacta
      661 tttgcaaaga gtatccaact gttgttccaa cagtatgatg aagtagatgt catagatacg
      721 attacttctc attttaacga tgtaacgatt gatttgtcta agtacgatat tatattgctt
      781 gatattaatt taacaaatat ttctaaagaa aatggattgg agatagcaaa agaactcata
      841 cagtctactc ctcatttgaa agttgttatg ttgacaggat atgttaaatc aatctataga
      901 gagagggcaa aaaaagtagg agcatacggt tttgtagata aaaatataga ccctaaacaa
      961 ctcatttcta tcttaaaaaa agtggattct ggaaaaaagt attttgaaca aatcgaatct
     1021 caggattatg ttgaatcttt aactgaccag gaaatagcta tcttaaattt gagtaagaaa
     1081 ggattctcta taaaagaaat agaggaaacg ttacaaatta gtaggagaac agtatttaat
     1141 catttgaccc acatctactc aaagcttttg gtaaataata agcaagaggc tatctacaaa
     1201 gccgaacaat tagggtattt tatggatttc tgagttgcag ataatcaaac gacataagtt
     1261 ttacttgtgt cgttgctagg gatttcctgt taaataccca gatttaaaat cattgttttt
     1321 taagttcttt ttgattgaaa ttgattgaga taacgctttt ataaaaagta gggcaatgcc
     1381 tgatatgaaa agctttttaa atttagtcaa acaaaagttg tttaaaccag gtctaaaaaa
     1441 actcgtaaag cttcacaact cccagaacgt taatatatgc ttatatatca acgattggaa
     1501 ctactttatg gttcgcacca tggtttttgt ggaaggatca aaagttgtcc tgaaaatttc
     1561 tcttaaccgt gtttaagctt tctaaacgtt tgagaaatca ttgtaacttc aaataactat
     1621 tttaaataaa acaaagaaac ggacaaagtt agatgagatg attctatttt gatttatagg
     1681 tgaagaagga gaatcttgtt tgataagtac tcaataaagg ttcactactc aattttaaat
     1741 aaaacgccac agtagtaatc atctactgtg gtgttttttg gtcttttagt ttctaaaagt
     1801 atccaattcg tcttgtgctt ctttggttgt tccgactagt tgtttcatga gttgatctag
     1861 tttggcattt gttttggcaa tttcttgatc aatgtcactg agctgtttag ctaattctgg
     1921 cagtggttta actgggactt cctcaaaggt atcaacatag cgtgggatat ttaagttata
     1981 gtcattttca atgatttcat caaatgaggc taaatatgaa aacttatcag aattatcacg
     2041 actcttataa gcatcaagga tctttttgat atggttatct gtcattgtgt tttgattttt
     2101 acccttatca aactcttttg aagcatcgat aaagaagaca tctttgttgg ttctgttctt
     2161 ctttaatatg ataatagtcg tagggatgct tgtattataa aagatatttg atgggagtcc
     2221 gataatggta tcgatagccc cttgttcgag tagtttttgg cgaatcttgc cttctgcagc
     2281 tccacggaaa aggacaccgt gtggtaagac aatcgccata gttcctgtat tttttaaatg
     2341 atagaaacca tgaagtaaaa aggcaaaatc ggcttttgat tttggtgcta atacaccata
     2401 acttgaaaac cgtggatccg ttaagaaacc agcagtcgct gaccatttca aagaataagg
     2461 tgggttcatc aacaccccat caaagttggt tggttcatct gttggccagt ctgcatccaa
     2521 ggtatcagcg ttacttaagt gttggttctc gatggcaacg ccatgaagca tcatgttcat
     2581 acgagccaag ttataagtag aagtattgat ttcttgacca tagtaagaaa cggtatcgga
     2641 ttggttacta tatttcttag catttaaaag gagtgatcca gatcccatag cagggtcata
     2701 gagggtcatt cctttttgat cttcacgacc taaaaagaca atctgagtca ttaagtgaga
     2761 gacagcttgt ggggtataga actcacctgc tttttttcct gattcactgg caaattcacc
     2821 aatcaagtac tcataagcat cgcctaaagt atccccatct actgcctcaa agtcaatttc
     2881 atttaatgtt ttcatgacat tggcaatggt ttgattttgt ttttgaggag tagagcctaa
     2941 ttttttagag tagaggtcaa tatcttcaaa taagttttca aagtcttcac cactttgttc
     3001 aatatcgcga aaaccttgag ctaatgattc taattggaaa gtattgtgat aaacatcttg
     3061 gatgagtttt tcaaaggtca gtgtcggttc gataaagtaa cctaaatcac cagtaacaac
     3121 agaaatgagg tcatctttta ggtcttcatc ttgataagca tcttcaaaga ttttttgggc
     3181 ctctgtaaag gtattaaagt gttgatctaa attatcacaa acagcaaaga gtaatttatc
     3241 agataaatgt ttgtaaaaaa taagtccaag aagatagttt ttatagtcat tggcatccat
     3301 ttggccacgt aattgatcgg ccgaatgcca tagtgcttgc cggagtgaag ttgttttctc
     3361 tgccatgtga gtcctttctt atacgaataa acgattgagt aatgtttgtt tgagtgcttt
     3421 gagttctgtt aatttatctt ctgtttgact catttgttgg tctaaggttt ggaagaagtc
     3481 accgatagct tcttgttcgg agagagaagg gaaatgaata gttaattctt taacaataga
     3541 accagataaa tttccttggc caccttgtaa atatttctta ataattgagg atttttgttt
     3601 atataaccaa ttttttataa aaagagaaga atattttttc tcaggaataa tggctaaaat
     3661 tgcttgatta atagctccac ttattcttga aagtccaacc tctccactag ttgcaccgta
     3721 gagagcgtat aataatgtat ttttttccac taattttgct gatgaatttg atagaccttt
     3781 gtctgtgata gataattctg tttgatcact gttgatctca gcagatctaa tgaaaggaat
     3841 attaccatta tagtattcag ggatacctac gttaggagtg ccacctgaaa acatccgaga
     3901 tatctccccc aatttcttct cttcccactc accatcaaaa ccttgtaaac ggatttcagg
     3961 gactttttgt ccttgagctg ggaacatttt cctgaggaag gtttgctttt gttctttaag
     4021 ggttgctagc ttttgatcct gcaactgaat taattgatca accgtttgga agagttcgcc
     4081 aatggcttct tgttcaggga gggagggaat ttgaatttta gcatatttga tatcactaga
     4141 gctaatgcta tcaaaagttg aacctgtact aattcttttc caataaccaa tttctttcaa
     4201 gtattttaat atttgaaaga taaattcatt accttttata gcagcaactc cacgcccaat
     4261 aataacatga taatttgtct ttccaacatc accaactgga gccctaacac taagaataat
     4321 atcacctttg tcagcttgtt ttgtaatttg tgttgtccag acacgcggaa aaacatatcc
     4381 attttttata tcggcatttc cttgtactag aatataatca gaagggtttg tagtataatt
     4441 ttgacttgat ggagattggc ccattgtaat ttgaacaata tcccctagtt ctttttcttc
     4501 ccactcccct ttaaaaccgt caaagcgata ttgaggctgt ttagattttg tcatctcttt
     4561 cctttcctag tgccctttta agggttggat ttgatctcta atgaattgaa ctaactcccg
     4621 ttcaaattgg ctcttgtatt tgagtttatt catggccgag tcaccttgtt tttctttata
     4681 ggcttggtag tctagggatt cttttaaggt tgacatgccc acttgtttgg tagagttagc
     4741 actatgataa ttagtcgcca caaaggctaa tttgtcagga tctgccttcc attggtccgc
     4801 aaagtgtttg atggcccttt ggatcttatc accaatgagg ttatctaact cataaacaat
     4861 gcttttccct tcataggctt tgggttcttt ttggatatct tgccataact cagctagtga
     4921 gtcagccata gcagggtttg attttgccaa atcttggatg tattgatcca tgggattgtc
     4981 attgagcctt tcttgaagag cttcttgttc ttgatcgaca aaggcttgaa ttaaggtcaa
     5041 gatatagtga tagttgatat catccatctg aatactttct agctcatagt cgatattgat
     5101 ctctgggatg gcttcgtcat catctcttcg ttttcgtatt tctgcaatga cattttggta
     5161 agtgccttta taagtatcaa tcacttcatc tgataaccca acttcactca agagtgtttc
     5221 attaaagtca ctatagacct gtacagatgc atagagctta tcaaatgctt gataggcttt
     5281 ggcgtattgt ttgagaaaag ctgtctgtgc gctctcaata gttgggaaag cgtctggatc
     5341 aggaactata ttttttaata ctgttacttt ttcaaggaat cttgcttttt cttcttccca
     5401 agaaggggcc aaaacgtcgt tttcaccccc attagagtag aggctaagcg ctttgtcgac
     5461 agcttcttta aaacgaagtg gtgtttggaa agtcacaacc tgaccgtaat gttttctact
     5521 ttcaaagata cggttggttc gtgagaaagc ttgaataatg tgctgaggtt tcataggctg
     5581 gcgatcaata aagatcgttg ataaacaagg cgcatcaaaa cctgttaaga gacggtcaac
     5641 cacaatcacg aggtctaatt gttcatgacg gtctttgaat ttatcttttt tacgggctaa
     5701 gcgatcattt aaatctctgt tatagccttg taagttatcg atggtaaagt tagtgccaaa
     5761 gaggtgatta tagtcttcta gatttttggt cattttatct tgtcggctga tagacgcatt
     5821 gtcattttca gtgatagaat aagtaatagc tacctttgga aagtctggta gtttttcttt
     5881 gaccttctta gaaatgacta aatccgtttc accagcttta actttctgaa tcagatcata
     5941 ataggcttgg gcacgagcaa tactcttaac ggttaataag ccttcgaatg tctgaccaat
     6001 cccattatta aagccaagct tcttacgtga ttgattaatg atactattta agacagctaa
     6061 catatgttct tcatgatcat aagcttcttc aggaatactg tcttcaggca tatcaggaat
     6121 ggttgtctta tactcaactt ggaagcctag aaccgcttta tcgtgaatgg cttctttgac
     6181 agtgtattga tggagacatt tgccgtattg ctgttccgtt gtttgggcta aatcacctaa
     6241 ttgagcacgc ttattttcca caaagatagg agtacctgta aagccatacc agcaactgtt
     6301 tcgaaaagta ttcgtgagat gacgctgccg ttctggcgtg acagcacggt ggcattcgtc
     6361 tacaacaaag acgacattta ggtgtgcaag acgctctttt agtttcttga attttggagt
     6421 gtcataactt tccatctgac tgatcatagc attgagtttt tgaatggtgg tgaccacaac
     6481 acgacgatca ctggattcta aatttttgat gagttgtctc gtatcttctg tctcatcgac
     6541 atcaaagata tcattttgcg cataagattg aaaagcagaa gtggtctgat tatctaaatc
     6601 ctttcggtca atgacaaaga tggatttttc aacggccggt atttgaagga tattgcgagc
     6661 cactttatag gaagttaagg tttttccaga acctgttgtg tgccagatat agccagactt
     6721 gcggtgacga ctcgcttcag cgacggcttc gattgcatga atctgataag gacgaaggag
     6781 aattagtgct tttttatcat catcaatgac agagtaagtc atgaccattt ggtgagcaag
     6841 agggatagat aggacttctt tggcaaaagc aaagagatct ttttgtggtt tgttgttttg
     6901 atctacccat tgggtcaaga agttaggatt taatttattt tctttagctg cagcgatata
     6961 ccttgtatct gttttatttg agaccacaaa catttgtaag gttgaaaaga tacctctaaa
     7021 ctggccttct ttatcatact ttttgacttg gttaaaagct tcaatgcatt gatgattctg
     7081 agattttaat tcaatttgga tcataggtaa gccattgatg agaagtgtca catcacctcg
     7141 tctatcacga gaaccagaga aagcaacttg attggcaatc tcataaacag aagttccacc
     7201 agccacattg tctgctttaa cgacttctaa acgaatcgct cctagttttg catcttcgcg
     7261 ttgcacttgt actttagcaa tgccgttttc accagctaac catttagccg cttcatagta
     7321 attgacaaag ttaagttgat tcttaatctg ctctttttca ctagctgtta aaggctcctc
     7381 attcagatat tgcgtgttat tttgcgctaa gatcttaaag aaattatccc ataaagcatc
     7441 ttcagttttt aattctttac gatatgtcca ttgactttca cctgtttcta agagatggat
     7501 caactctttt tcaagttcaa gttccgtttt agtttgattt cccattttta atccccattc
     7561 gacatgatat agtagtatta tatcattggc ggatcctttt tagaatggca aagctattga
     7621 aaccttccaa acagtctttt tttgattagt tgaatgctta agatcctagc aactaaacaa
     7681 agctatgact atgtaaatgc aatcgagaaa gttcttgtat ttttgagttt gcttgacaat
     7741 aatttaaggt gaagaaagta ataagacaac actagtaaaa aggtcgagtt ctcaaaatag
     7801 ataaaaaact taagacagat ctagcttgac tattaatagg gttttaaagg acgataatct
     7861 gctacaatat acagaaaagg aatttattga agatatgaaa attttggtag ccatagattc
     7921 ttttaaggga tcggtaacgt cttcagaact taacactagt gtagctcaag cccttctttc
     7981 tgtcgataag cagttagtta ttgaagcaag agctatcgct gatggagaag aaggaagttt
     8041 agcagccctc tcacaaaccg tggcggggag gtggcatcaa gtaaaaaccg ttgatctatt
     8101 aagacgcccg attaaagtcg cttattatcg tcacgcaaag caagctttta ttgaatctgc
     8161 gagtattatt ggtattgata ggatcacccc cagttcagta acctatgcac aagccacttc
     8221 ctacggtttg ggtttggcag ttaaagatgc cattcaaaaa ggagctactc aaatcgaaat
     8281 catgctagga ggaacaggca cttctgatgg tggcaaaggg tttttagaaa gtttaaacta
     8341 tgatttcatg acaggaagat cttatcttga tactttagca tcacctgtta cgctattagg
     8401 tctgactgat gtaaccaatc catatcatgg ccctcaagga tttgctgcgg tctttggccc
     8461 tcaaaaaggt gggagtctta gtcaaattga agaaacagat caaattgcaa gtaactttgc
     8521 taaaaaagtt ttttaccaaa caaccattga tttacagaca ataccaggaa gtggagcagc
     8581 tggaggatta ggaggtgcca ttgtcctatt aggaggcacc ttaactagtg gttttagcag
     8641 gattgcagag ctactaaatc tggataattc ccttcagtct tgtgatttag ttattactgg
     8701 agaaggatgc ctggatacac aatcacaatc tggcaaggta cctgttgcga ttgcaagaat
     8761 ggctaaaaaa tattaagttc caacgattgc tctatgcggt tctgtaaaga ttgagacagg
     8821 actagcagca gaagactttt tggctgtttt ttcaatacaa cagcagccaa tttccttaga
     8881 agcagcgata gataaaataa caacactttc taatatcaaa atattagcag ctaaccttat
     8941 gctattgata gctcagttta ataagtaaac tcatctattt tcgattgtgt agcattcatt
     9001 tggcggttaa aagtaaccta tgaagaagga gacgcacgag ctaactcatt agcatagt
//
LOCUS       DAUCLR010000075         9027 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.075, whole genome shotgun sequence.
ACCESSION   DAUCLR010000075 DAUCLR010000000
VERSION     DAUCLR010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9027
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.075"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            90..1469
                     /locus_tag="VGT87_001462"
     CDS             90..1469
                     /locus_tag="VGT87_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEQ3814299.1"
                     /translation="MSIKEQTDNNELENGMVRGLENRHVQLIAIAGTIGTGLFLGAGR
                     SIALTGPSIIFVYMITGAFMFMMMRAIGEMLYYDPDQHTFINFISKYIGPGWGYFSGL
                     SYWISLIFIGMAEITAVGAYVQFWFPSWPAWLIQLVFLVLLSSINLIAVRVFGETEFW
                     FAMIKILAILALIATAIFMVLTGFETHTGHASLSNIFDHFSMFPNGKLKFFMAFQMVF
                     FAYQAIEFVGITTSETANPRKVLPKAIQEIPTRIVIFYVGALVSIMAIVPWHQLPVDE
                     SPFVMVFKLIGIKWAAALINFVVLTSAASALNSTLYSTGRHLYQIANETPNALTNSLK
                     INTLSRQGVPSRAIIASAVVVGISALINILPGVADAFSLITASSSGVYIAIYALTMIA
                     HWKYRQSKDFMADGYLMPKYKVTTPLTLAFFAFVFISLFLQESTYIGAIGATIWIIVF
                     GIYSNVKFK"
     gene            1639..3099
                     /locus_tag="VGT87_001463"
     CDS             1639..3099
                     /locus_tag="VGT87_001463"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEQ3814300.1"
                     /translation="MYKDDSLTLHTDLYQINMMQVYFEQGIHNRHAVFEVYFRKEPFN
                     NGYAVFAGLQRMVEYLEQFQFSETDLAYLEELGYPENFLTYLKELRLELTIRSAKEGD
                     LVFANEPIVQVEGPLGQCQLVETALLNIVNFQTLIATKAARIRSVIEDEPLLEFGTRR
                     AQELDAAIWGTRAAIIGGADATSNVRAGKRFGIPVSGTHAHALVQAYGNDYDAFMAYA
                     KTHKDCVFLVDTYDTLKVGVSTAIRVAKEMGDKINFLGVRLDSGDLAYLSKTVRQQLD
                     DAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVSIE
                     QEDGSMRDTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITFTDINVNELTEIEM
                     FHPTYTYIKKTVRNFDAIPLLVDIFVKGELVYQLPTLSEIKTYAKKEFDKLWDEYKRV
                     LNPQDYPVDLARDVWQNKMALIDNIRKDTHGKSDLK"
     gene            3096..3920
                     /gene="nadE"
                     /locus_tag="VGT87_001464"
     CDS             3096..3920
                     /gene="nadE"
                     /locus_tag="VGT87_001464"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEQ3814301.1"
                     /translation="MTLQEEIIRQLGVKASIAPQEEIRKTVDFLKAYLRKHLFLKTYV
                     LGISGGQDSTLAGKLAQMAIAELREETSDQAYQFIAVRLPYGVQADEADAQKALAFIM
                     PDQTLTINIKAAVDGQVEALQAAGVEISDFNKGNIKARQRMISQYAIAGQMAGAVIGT
                     DHAAENITGFFTKFGDGGADILPLFRLNKRQGKALLKVLGADAALYEKVPTADLEDQK
                     PGLADEVALGVTYQDIDDYLEGKLISKVAQATIEKWWHKGQHKRHLPITIFDDFWK"
     gene            4105..5442
                     /gene="pepC"
                     /locus_tag="VGT87_001465"
     CDS             4105..5442
                     /gene="pepC"
                     /locus_tag="VGT87_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEQ3814302.1"
                     /translation="MSALTETFTEQLFAHYEASAKFSAIENAVTHNGLLKSLETRQSE
                     VDNDFVFSIDLTKDKVSNQKASGRCWMFAALNTFRHKLITEFKLENFELSQAHTFFWD
                     KYEKANWFMEQVIATADQELTSRKVKFLLDVPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESISSSNSRELNQYLNKLLRQDAQILRDLIASGAKADQVEDRKAELLQEIFNFLAMTL
                     GLPPRHFDFAYRDKDDHYHVEKGLTPQAFYDKFVGLKLSDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSREVRYLNLDMKRFKELAIKQMQAGESVWFGSDVGQVSDRQKGILATN
                     TYDFEASMDINLSQDKAGRLDYSESLMTHAMVLTGVDLDETGKPLKWKVENSWGEKVG
                     DKGYFVASDAWMDEYTYQIVVRKEFLTADELAAYEKEPQVLAPWDPMGALAQC"
     gene            complement(5907..8072)
                     /locus_tag="VGT87_001466"
     CDS             complement(5907..8072)
                     /locus_tag="VGT87_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEQ3814303.1"
                     /translation="MITIKNPKILKWLKYVLSAILSLIILVIIIGGLLFTFYISSAPK
                     LSEAQLKSTNSSLVYDGNNNLIADLGSEKRENVTADSIPINLVNAITSIEDKRFFNHR
                     GVDLYRIFGAAFHNLTSQTTQGGSTLDQQLIKLAYFSTNESDQTLKRKAQEVWLALQM
                     ERKYTKQEILTFYINKVYMGNGNYGMLTAAKSYYGKDLKDLSYAQLALLAGIPQAPSQ
                     YDPYLHPEAAQNRRNVVLQQMYMEKHLTKAEYETAIATPVAEGLRSLQQRSTYPKYMD
                     NYLKQVIEEVKKETNKDIFTAGLKVYTNIIPDAQQTLYNIYHSGDYVYYPDQDFQVAS
                     TIVDVTNGHVIAQLGGRNQDENVSFGTNQAVLTDRDWGSTMKPITAYAPAIESGVYTS
                     TAQSTNDSVYYWPGTTTQLFNWDLRYNGWMTIQAAIMLSRNVPAVRALEAAGLDYARS
                     FLSSLGINYPEMHYSNAISSNNSSSDKKYGASSEKMAAAYAAFANGGIYHKPRYVNKV
                     EFSDGTSKTFDEKGKRAMKETTAYMMTDMLKTVLTYGTGTAAAIPGVAQAGKTGTSNY
                     TDEELAKIGEKYGLYPDYVGTLAPDENFVGFTKRYAMAVWTGYKNRLTPVYGSSLEIA
                     SDVYRSMMTYLTNGYSEDWTMPNGLYRSGGFLYLSGTYASNTDYTNSVYNNLYSNNTT
                     TASSQTTSDDTSSSNDISNSTNTDNNSNHPSTDDKKTTH"
     gene            complement(8059..8667)
                     /gene="recU"
                     /locus_tag="VGT87_001467"
     CDS             complement(8059..8667)
                     /gene="recU"
                     /locus_tag="VGT87_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEQ3814304.1"
                     /translation="MVNYPHNLIRQKVSSVQKQTKQNKVDFANRGMSFEAAINATNDY
                     YLSRQIAVIHKKPTPVQIVKVDYPKRSRAKIVEAYFRQASTTDYCGVYKGHYVDFEAK
                     ETRQKTAMPMKNFHLHQIEHMACVLHQKGICFVLLHFSTLKETYYLPAQALISFYQID
                     NGSKSMPIDYIRKNGFKVAFGAFPQVPYLNIIEQNFLGGDYN"
     gene            8748..>9027
                     /locus_tag="VGT87_001468"
     CDS             8748..>9027
                     /locus_tag="VGT87_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 family protein"
                     /protein_id="HEQ3814305.1"
                     /translation="MTAILITGYRSFEIGIFDHKDPRVSIIKQAIRKDLIGYLENGVD
                     WFIFTGNLGFEQWALEVANELKEEYPLQIATIFLFETHGDKWNEKNQEV"
BASE COUNT         2700 a         1577 c         1867 g         2883 t
ORIGIN      
        1 agttttcctt aatatctttc ctagataaat gttgaaaatt ttctgaaaaa aggatataat
       61 aaatagtatt ttagtgaatg gagtttagaa tgtctataaa agaacaaaca gacaataacg
      121 aactagaaaa tggaatggta aggggcttag aaaatcgtca tgtgcagtta attgcgattg
      181 caggaacgat tggaacaggt ctctttttag gggcaggacg ctctattgcc ctaacaggac
      241 catctattat ttttgtgtat atgattacag gtgccttcat gtttatgatg atgcgtgcca
      301 tcggtgaaat gctctattat gatccagatc aacacacctt tatcaatttt atttccaaat
      361 acattggtcc aggctggggt tatttttcgg gtctatccta ttggatttcc cttattttta
      421 ttggaatggc agaaatcaca gcagtaggtg cttatgtgca attttggttc ccaagctggc
      481 cagcctggtt gattcagttg gttttcttag tcttacttag ttcgatcaac ttaattgctg
      541 tgcgcgtttt tggggaaaca gagttttggt ttgccatgat taagatttta gctattttag
      601 ctttgattgc aacagctatt ttcatggtat tgacaggttt tgaaactcac acaggccatg
      661 ctagcctttc caatattttt gaccattttt ccatgttccc aaatgggaaa ctaaagttct
      721 ttatggcctt ccaaatggtt ttctttgctt atcaagctat cgaatttgtg gggattacca
      781 cttctgagac ggctaaccca agaaaagttc taccaaaggc tattcaagaa attccaaccc
      841 gtattgtgat cttttatgtg ggagccttgg tctctattat ggcaattgtg ccatggcatc
      901 agttgccagt tgatgaatct ccttttgtga tggtgttcaa attgattggt attaaatggg
      961 cagcagcctt gattaacttt gtggtcttga catcagcagc gtcagcgctt aactcaaccc
     1021 tttattcaac cggtcgtcat ctctatcaga ttgctaatga gactccaaac gctttgacca
     1081 atagcttaaa gattaatact ttatctcgac aaggggtgcc aagtcgtgcc attattgctt
     1141 cagcggttgt ggttggtatc tcagctttga ttaatatttt gccaggagtt gcagatgcgt
     1201 tctcactcat tacggcctcc tcatcaggtg tatacattgc tatctacgcc ttaaccatga
     1261 ttgctcactg gaagtaccgt caatctaagg actttatggc agatggttat ttgatgccaa
     1321 aatataaagt gacaacgcct ttgacccttg ctttctttgc ttttgtcttt atctctcttt
     1381 tcttacaaga atccacctat atcggcgcaa ttggagcaac gatttggatt attgtttttg
     1441 ggatttatag caatgtcaaa tttaaataat aattagcgat atagaactga atccattaac
     1501 tttttcttat ttgaggatgt tatgatggat cggttctttt ttactttagc gataactcaa
     1561 gttatttcta aatcctagcc aaagagtcct ttttaaaata tgttataata gtaacaataa
     1621 ttcaaaggag tcccctttat gtataaagat gatagtttaa ctctccatac tgatttgtat
     1681 cagattaata tgatgcaagt ttattttgag cagggcattc ataatcgtca tgcggtgttt
     1741 gaagtctatt tccgcaagga accttttaac aatggttatg ctgtttttgc cggtttacag
     1801 cgcatggtgg agtacctaga gcaatttcaa ttttcagaaa cagatttggc ttaccttgaa
     1861 gaactgggtt atccagaaaa ctttttgacc taccttaaag agttacgatt agaattgacc
     1921 atccgttcag ctaaagaagg agatttggtt tttgctaatg agccgattgt gcaagtagaa
     1981 ggccctctag gtcagtgtca gttggtcgaa acagctcttt taaacattgt taacttccag
     2041 accttgattg ctacaaaggc tgctcgtatt cgttctgtga ttgaggatga accccttttg
     2101 gaatttggca cccgtcgtgc acaggaacta gatgctgcga tttggggaac ccgcgcagcg
     2161 attattggtg gagcagatgc aacaagtaat gtgagagcag gtaaacgctt tggtatccct
     2221 gtttcaggta cgcatgctca tgctttagtg caagcctatg gtaatgatta tgatgctttc
     2281 atggcttatg ctaagaccca taaagattgt gtgtttcttg ttgataccta tgacacactt
     2341 aaagtaggtg tgtcaacagc tatccgagta gccaaagaaa tgggtgacaa gattaatttt
     2401 ctaggtgttc gattggactc tggtgacttg gcttacctct caaaaacagt ccgtcagcag
     2461 ttagacgacg ctggttttac cgaggctaag atctatgctt ctaatgacct cgatgaaaat
     2521 accatcctca acttaaaaat gcaaaaggcc aaaattgatg tttggggtgt tgggaccaaa
     2581 ctgatcacag cctatgatca gcctgcctta ggtgctgttt ataagattgt ctctattgag
     2641 caagaagatg gtagcatgcg tgatacgatc aaattatcaa ataatgcaga aaaagtatcc
     2701 acaccaggta aaaaacaagt ttggcgcatc accagtcgtg aaaaaggcaa atcagaaggc
     2761 gactatatca cgtttactga tattaatgtt aatgaattaa ctgagattga gatgttccat
     2821 ccgacctata cctatattaa gaaaaccgtc agaaattttg atgcaattcc tctcttagtt
     2881 gatatttttg tcaagggaga attagtttac cagctaccga ccttatctga aatcaagact
     2941 tatgctaaaa aagaatttga caagctttgg gatgaataca agcgtgtctt aaatcctcaa
     3001 gactacccag tagacttggc acgagatgtt tggcaaaata agatggcctt aattgataac
     3061 attcgtaaag atactcatgg aaagagtgat ttaaaatgac cttacaagaa gagattatcc
     3121 gacaattagg cgtcaaggct agcattgccc cacaagaaga aattcggaaa acagttgatt
     3181 ttttaaaagc ttatctgcgt aagcacttat ttttgaaaac ttatgtctta ggcatttctg
     3241 gaggtcagga ttctaccctc gcaggaaagc tagcccagat ggccattgca gagttacggg
     3301 aggaaacaag tgatcaagct taccaattta tagctgttcg cttgccttac ggggttcagg
     3361 ctgatgaagc agatgcgcaa aaggccttgg cttttatcat gccagaccaa acactgacta
     3421 ttaatattaa agcagctgtt gatggccaag tagaggctct acaagcagca ggcgttgaga
     3481 tttctgattt taacaaaggt aatatcaaag cacgtcagcg gatgatcagt caatatgcca
     3541 ttgctggcca aatggctggt gctgttattg gaacggatca tgcggcagaa aacatcactg
     3601 gtttctttac taaatttggt gatggtggag cagatattct tcccctcttt aggcttaaca
     3661 aacgtcaagg aaaggctctt ttgaaagttt taggagcaga tgctgctttg tatgaaaaag
     3721 ttccaactgc tgatttagaa gatcaaaaac caggcttggc tgatgaggtt gctctagggg
     3781 tgacctatca agacatcgac gactatttgg aagggaagct gatttccaaa gtagctcaag
     3841 caactattga aaaatggtgg cataaaggac aacacaagcg tcacctgcca atcaccattt
     3901 ttgatgattt ttggaaataa gagatgatgt tcaaagagta aaaaaagaaa acaaaaaaag
     3961 ttaggttatc ctaacttttt tcttgaagaa aatcaaataa ggtctataat agaagtaatc
     4021 ctttagaata gaatggctgg ataataacca tcttgccaat attatttcag gtgaagctta
     4081 tcttaatatt ttaggaggac aataatgtca gcattaacag agacatttac agaacaatta
     4141 tttgctcatt acgaagccag tgcaaaattt tcagctattg agaatgccgt tacgcataat
     4201 ggtttgctaa aatcattaga aacccgtcaa agtgaagtgg acaatgattt tgtgttttct
     4261 attgatttaa ctaaagataa ggtatctaac caaaaggctt ctggtcgttg ctggatgttt
     4321 gctgccttga acactttccg acacaaatta atcacagagt ttaaattaga aaattttgaa
     4381 ctgtcacaag ctcatacttt cttttgggat aagtatgaaa aagctaactg gtttatggag
     4441 caggtcattg ctacagctga ccaagaatta accagtcgta aagttaagtt tctcttggat
     4501 gttccacaac aagatggtgg tcagtgggac atggtagttt ccttgtttga gaagtatgga
     4561 gtggtgccaa aatctgttta cccagaatca atctcatcaa gtaacagtcg tgaattaaac
     4621 cagtatttga ataaattatt gcgccaagat gctcaaattc ttcgtgacct gattgcgtca
     4681 ggtgctaagg cagaccaagt tgaggatcga aaagcagagc ttcttcagga aatttttaac
     4741 ttcttggcca tgacccttgg tttaccacct cgtcattttg attttgccta ccgtgataaa
     4801 gatgaccact atcatgttga gaaaggtttg acaccgcaag cgttttacga caaatttgtt
     4861 ggcttaaagc tttctgatta tgtgtctgtt attaatgcgc caactgctga taagccttat
     4921 ggcaaatcat acactgttga gatgctagga aatgtggtcg gtagtcgtga ggttcgctac
     4981 ctcaaccttg atatgaaacg tttcaaagaa ctagctatca agcagatgca ggctggtgag
     5041 tccgtttggt ttggctctga tgtgggtcaa gtttcagatc gtcaaaaagg gattttggca
     5101 actaatactt acgattttga agctagtatg gatatcaatc ttagtcaaga taaggctgga
     5161 cgtttggatt atagtgaaag tttgatgaca catgctatgg ttttaacagg tgttgactta
     5221 gatgaaacag gaaaaccgtt aaaatggaag gttgaaaatt catggggtga gaaggttggt
     5281 gacaaaggtt actttgttgc ttcagatgct tggatggatg aatataccta tcaaattgtt
     5341 gtccgtaaag aattcttaac agctgacgag ttagcggctt atgaaaaaga gccacaagta
     5401 ctagctcctt gggatccaat gggtgcttta gcacaatgct aattcctttc tttgcgactg
     5461 tatgaaatct aaaggctgga caaaacgaac agcaaacgga ttggactccg cataagaaag
     5521 tgaacatgaa aaaaattatt atgctaagaa aatgacatat gaaaaaacac gtcttcggta
     5581 tatggaaacg tgttttttca tgtttactct cttatggcat taggttgcta tttataacaa
     5641 ttttaaataa gtctattttg ttttaggtta acgtttgaaa aatcagttgc taatcccctt
     5701 aacgttgtac cttttcttta atacgattaa agaattctgt tttttgtttt atattgtatg
     5761 tggtaagtca tttgtgtcat ataaaaacga gtcctcacag aacgtgaact cgctttttat
     5821 ttagattatc acttaaggta gatattaaaa gtaactaaat ggatatgtaa taaaaggtag
     5881 gtacagacaa taagtagagt tagagattaa tgagttgtct ttttatcatc ggtagatgga
     5941 tgattgctat tgttgtctgt attggttgaa ttacttatat cattgctact actagtatca
     6001 tctgaagtcg tttggctaga agctgttgtc gtgttattgc tgtaaagatt gttgtaaacc
     6061 gaattagtat agtcggtgtt gctcgcatag gttccgctta agtagaggaa tccaccactg
     6121 cgataaagac catttggcat cgtccaatct tcactgtaac catttgttaa gtaagtcatc
     6181 atgctacgat aaacgtcaga tgcaatctct agacttgatc cgtatactgg ggtcaagcgg
     6241 tttttgtaac ctgtccaaac agccatggcg tagcgcttag taaagccaac aaagttttcg
     6301 tctggcgcta atgtaccaac ataatctgga taaaggccgt atttttcacc aattttagct
     6361 agttcctcat cagtgtagtt agaagtccct gttttaccag cttgcgcaac accaggaatg
     6421 gcagcagcag tacctgtacc ataagtgaga acagttttta acatatctgt catcatatag
     6481 gccgtggttt ctttcatggc acgttttcct ttttcatcaa aagttttact tgtaccatca
     6541 ctaaattcca ctttattgac ataccttggt ttatgataaa taccaccatt tgcaaaagca
     6601 gcgtatgcag cggccatttt ttcactactt gcaccatatt ttttatctga gctactgtta
     6661 ttacttgaga tagcgtttga gtagtgcatt tcgggatagt taatacctaa actgcttaag
     6721 aaagatcgag catagtcaag tcctgcggct tccagtgctc ggactgctgg gacatttcgc
     6781 gatagcataa tagcagcttg gattgtcatc catccgttat atctaaggtc ccagttaaac
     6841 aattgggtag tggttccagg ccaataatag actgagtcat tagtcgactg agcagtagaa
     6901 gtataaacac cagattcaat agcaggagca taggctgtga ttggcttcat ggtagaaccc
     6961 cagtcacgat cagttaaaac agcttggtta gtcccaaatg aaacattttc atcttgatta
     7021 cgtccgccaa gctgagcaat aacatgacca tttgtcacat caacaatcgt tgaagcaact
     7081 tggaaatctt ggtctggata gtaaacataa tcaccagaat gataaatatt ataaagagtc
     7141 tgctgcgcat cggggataat attggtataa acttttaaac cagcggtaaa aatatcttta
     7201 ttcgtttctt ttttgacttc ttcaataact tgttttagat aattatccat atattttgga
     7261 taagttgagc gctgttggag tgatcgtaga ccttcagcga cgggagttgc gatggcagtt
     7321 tcatattctg ctttcgtcag atgtttttcc atgtacatct gttgcaacac gacgttacgg
     7381 cgattttgag cagcttcagg atgaaggtaa ggatcatatt gactaggagc ttgagggatt
     7441 ccagccaata gggctagttg ggcataagat aaatccttaa gatccttgcc ataataagac
     7501 ttagcggctg tcagcatacc atagttgcca ttacccatat atactttgtt gatgtaaaaa
     7561 gtcaggattt cttgtttagt atattttcgc tccatttgaa gagcaagcca aacttcttga
     7621 gccttacgtt ttaaggtttg atcagattca ttagtagaaa aataggctag tttaatgagt
     7681 tgctgatcaa gcgttgaccc cccttgagtg gtctgactcg ttagattatg aaaggcagca
     7741 ccaaaaatac gataaagatc tactccacga tggttaaaga aacgtttatc ttcaattgag
     7801 gtaatagcat taactagatt aatagggata ctatcagctg ttacattttc acgcttttca
     7861 gaacccaaat cagcaatcag attgttatta ccatcataaa ccaagctaga gtttgttgat
     7921 tttaactggg cttctgacag tttcggagca ctgctaatgt agaaggtaaa caaaagacca
     7981 ccaataataa taacaaggat aataaggcta agaattgcac ttaatacata ctttagccac
     8041 ttaaggattt ttggattttt aattgtaatc accgcctaaa aaattttgtt caattatgtt
     8101 taaataaggg acttgtggaa aggctccaaa tgctacctta aagccatttt ttctgatata
     8161 atcaatgggc atcgatttac tgccattatc tatctgataa aatgaaatca aggcttgagc
     8221 aggtaggtag taagtctcct tcagtgtgga aaagtgaaga aggacaaagc agattccttt
     8281 ttgatgtaaa acgcaagcca tatgttcaat ctgatgtaaa tggaaatttt tcatgggcat
     8341 agctgttttt tgcctggttt cttttgcttc aaaatcaaca taatgtcctt tataaacgcc
     8401 gcaatagtca gtcgttgaag cttgtctaaa gtacgcttca acgatttttg cccgactgcg
     8461 tttagggtaa tccactttga caatttggac aggagtaggt tttttgtgaa tgacagctat
     8521 ttgacgagat aaataataat catttgtcgc gttgatagca gcttcaaagg acatacctct
     8581 attggcgaag tccaccttat tttgctttgt ttgcttttga acggaactta ctttttgtcg
     8641 aataagatta tgaggataat taaccattgt tctcctaata ttatctagtt aatgctaaaa
     8701 atttaagcac ttttattata acatattttt tgaaaggata acacctaatg actgcgattc
     8761 taattacagg ttaccgaagc tttgaaatag ggatttttga ccataaagac ccacgagttt
     8821 caattattaa acaggctatt cgtaaagatt taataggtta tttggaaaat ggtgtggatt
     8881 ggtttatctt tacagggaac ttgggttttg aacaatgggc tttggaagta gctaatgagc
     8941 taaaagagga gtatcccttg cagatagcta ctatattctt gtttgaaacg catggagata
     9001 agtggaatga aaaaaatcaa gaagttt
//
LOCUS       DAUCLR010000076         8992 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.076, whole genome shotgun sequence.
ACCESSION   DAUCLR010000076 DAUCLR010000000
VERSION     DAUCLR010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8992
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.076"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(88..1401)
                     /locus_tag="VGT87_001469"
     CDS             complement(88..1401)
                     /locus_tag="VGT87_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEQ3814306.1"
                     /translation="MTIIIMDSNSAHETDNLSVSFLNFCYNSLMKRHFLLLTFYLFLT
                     GLTAGLVAFILTKAIHLIQSLSFGFSQGSFSTMIASVPPQRRALSLLFAGLLAGLGWH
                     LLAKKGKDIQSIQQIIQDDISFSPWTQFWHGWLQLTTVSMGAPVGREGASREVAVALT
                     SLWSQRCNLSKADQKLLLACASGAALGAVYNAPLATILFILEAILNRWSLKNIYAACL
                     TSYVAVETVALLQGRHEIQYLMPQQHWTLGTLIWSVLAGLILSLFAHAYKHLLEHLPK
                     ADARSQWFIPKVLIAFSLIAGLSIFFPEILGNGKAGLLFFLHEEPHLSYISWLLVTKA
                     VAISLVFASGAKGGKIAPSMMLGGASGLLLAILSQYLIPLSLSNTLAIMVGATIFLGV
                     INKIPLAAPVFLVEITGQSLLMIIPLALANLIFYFSYQFYRFILK"
     gene            complement(1376..2335)
                     /gene="nrdF"
                     /locus_tag="VGT87_001470"
     CDS             complement(1376..2335)
                     /gene="nrdF"
                     /locus_tag="VGT87_001470"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEQ3814307.1"
                     /translation="MTTYYEAINWNEIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSLQEKDLVGKVFGGLTLLDTMQSETGVEAIRADVRTPHEEAVLNNIQFMESVHAK
                     SYSSIFSTLNTKKEIEEIFEWTNNNEFLQEKARIINDIYANGDALQKKVASTYLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEDEQENFR
                     DWMYDLLYQLYENEEKYTKTLYDGVGWTEEVMTFLRYNANKALMNLGQDPLFPDTAND
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGSVEAMSDDDYNYGL"
     gene            complement(2539..4698)
                     /gene="nrdE"
                     /locus_tag="VGT87_001471"
     CDS             complement(2539..4698)
                     /gene="nrdE"
                     /locus_tag="VGT87_001471"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEQ3814308.1"
                     /translation="MSLKDLGDISYFRLNNEINRPVNGKIPLHKDKEALKAFFAENVL
                     PNTMSFTSITEKIEYLISNDYIESAFIQKYHPEFITELASIIKSENFRFKSFMAAYKF
                     YQQYALKTNDGEHYLENLEDRVLFNALYFADGQEDLAKDLAVEMINQRYQPATPSFLN
                     AGRSRRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     AGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGITVPDKFYELARKNEDMYLFSPYNVEKEYGIPFNYLDITNMYDELVANPKI
                     TKTKIKARDLETEISKLQQESGYPYIINIDTANKANPIDGKIIMSNLCSEILQVQTPS
                     LINDAQEFVEMGTDISCNLGSTNILNMMTSPDFGRSIKTMTRALTFVTDSSNIEAVPT
                     IKHGNSQAHTFGLGAMGLHSYLAQHHIEYGSPESIEFTDIYFMLLNYWTLVESNNIAR
                     ERQTTFVGFENSKYADGSYFDKYVTGHFVPKSDLVKSLFKDHFIPQASDWEALRDAVQ
                     KDGLYHQNRLAVAPNGSISYINDCSASIHPITQRIEERQEKKIGKIYYPANGLSTDTI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFLRSELPMELYEWKTQSKQTTRDLS
                     ILRNYAFNKGIKSIYYIRTFTDDGEEVGANQCESCVI"
     gene            complement(4718..4936)
                     /locus_tag="VGT87_001472"
     CDS             complement(4718..4936)
                     /locus_tag="VGT87_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin NrdH"
                     /protein_id="HEQ3814309.1"
                     /translation="MITVYSKNNCMQCKMTKKFLEQHGANFQEINIDEHPEKVDYVKS
                     LGFTSAPVIEADNLVFSGFQPAKLKELI"
     gene            5329..5592
                     /locus_tag="VGT87_001473"
     CDS             5329..5592
                     /locus_tag="VGT87_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEQ3814310.1"
                     /translation="MASKDFHIVAETGIHARPATLLVQTASKFASDITLDYKGKAVNL
                     KSIMGVMSLGVGQGADVTISAEGADAEDAIAAIEETMTKEGLA"
     gene            5597..7330
                     /gene="ptsP"
                     /locus_tag="VGT87_001474"
     CDS             5597..7330
                     /gene="ptsP"
                     /locus_tag="VGT87_001474"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEQ3814311.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETVTVADTNAEEARL
                     DVALQAAQDELSVIRENAVESLGEEAAAVFDAHLMVLADPEMISQVKETIRAKQTNAE
                     TGLKEVTDMFITIFEGMEDNPYMQERAADIRDVAKRVLAHLLGVKLPNPATINEESIV
                     IAHDLTPSDTAQLNKQFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNDITKRVKDGD
                     VIAVNGITGEVIIDPSEDQVLAFKEAGAAYAKQKAEWSLLKDAHTETADGKHFELAAN
                     IGTPKDVEGVNDNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDLPKEMNPFLGFRALRISISETGDAMFRTQMRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADDIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWVGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDSAKMEEYANRALTECSTAEEV
                     LELSKEYVSED"
     gene            7515..8942
                     /locus_tag="VGT87_001475"
     CDS             7515..8942
                     /locus_tag="VGT87_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEQ3814312.1"
                     /translation="MAKQYKNLVNGEWKLSENEITIYAPATGEELGSVPAMTQAEVDA
                     VYASAKKALSDWRALSYVERAAYLHKAADILVRDAEKIGAILSKEVAKGHKAAVSEVI
                     RTAEIINYAAEEGLRMEGEVLEGGSFEAASKKKIAIVRREPVGLVLAISPFNYPVNLA
                     GSKIAPALISGNVVALKPPTQGSISGLLLAEAFAEAGIPAGVFNTITGRGSVIGDYIV
                     EHEAVNFINFTGSTPIGEGIGKLAGMRPIMLELGGKDSAIVLEDADLVLAAKNIVAGA
                     FGYSGQRCTAVKRVLVMDKVADQLAAEIKTLVEKLSVGMPEADADITPLIDTSAADFV
                     EGLIKDATDKGATALTAFNREGNLISPVLFDHVTTDMRLAWEEPFGPVLPIIRVTTVE
                     EAIKISNESEYGLQASIFTTNFPKAFGIAEQLEVGTVHLNNKTQRGTDNFPFLGAKKS
                     GAGVQGVKYSIEAMTTVKSVVFDIQ"
BASE COUNT         2858 a         1623 c         1878 g         2633 t
ORIGIN      
        1 ttcctcgttt ccacttaatt ttgtgtctac tttagtatac caagtccaat tcatccgaag
       61 ctttttatta aaaaagtaat taactaatca tttaaggatg aaacgataaa actgatagct
      121 aaaataaaaa atgaggttag ctaatgctaa agggataatc attagtaacg attggcctgt
      181 aatttcaacc agaaaaactg gtgccgccaa gggtatctta ttgatcacac ctaaaaagat
      241 tgttgcacca accataatgg ctaacgtatt tgatagagac aagggaataa ggtactgact
      301 aaggatagct agtagtaagc cactagcacc tccaagcatc atagaaggag caattttacc
      361 cccttttgct ccagaagcaa aaactaaaga gatagcgaca gctttggtaa caagtagcca
      421 gctaatatag ctcagatgtg gttcttcatg gagaaaaaag agtaatccag ctttgccatt
      481 tcctaaaatc tctgggaaga aaatgctgag tcctgcgata aggctaaaag cgatgagaac
      541 tttcggaata aaccactgac ttctggcatc agccttagga aggtgttcca aaagatgttt
      601 gtaagcgtga gcaaagaggg aaaggataag accagccagg acggaccaaa taagggttcc
      661 tagcgtccaa tgttgctgag gcattaggta ttgaatctca tgtcgtcctt gtaataaagc
      721 aactgtttcc acagccacat agctcgttag gcaggcagcg tatatatttt taagggacca
      781 acggttaaga atagcttcca agataaataa aatagtggct aagggagcat tgtagacagc
      841 accaagggca gcgccagaag cgcaagctaa taaaagtttt tgatcggctt ttgacaagtt
      901 acatctttgg ctccaaagag acgtcaaggc aacagcgact tcccgtgaag ctccttctcg
      961 gccgacagga gctcccatag atacggtcgt taattggagc caaccatgcc aaaactgtgt
     1021 ccagggacta aatgagatgt catcttgaat gatttgctga atagattgaa tgtcctttcc
     1081 ttttttagcc aataggtgcc acccaagacc tgcaagaagt ccagcaaaga ggagtgaaag
     1141 agctcgtctt tgtggtggaa cactagcaat catagtgcta aaagaacctt gactgaatcc
     1201 aaaactgagg gactgaatca ggtgaatggc ttttgttaag ataaaagcaa caaggccagc
     1261 tgttaaacct gttaaaaaga ggtaaaaggt caagagtaaa aagtgtcgtt tcataagact
     1321 attataacaa aagttaagaa aagagactga taaattatca gtctcgtgag cactattaga
     1381 gtccataatt ataatcgtca tcagacatag cttctacaga accaagaaga tagccattcc
     1441 ctacctgtga gaaaaagtca tggtttgatg ttcctgttga aataccattc ataacaattg
     1501 ggttaacatc gtttgctgta tctgggaata aaggatcctg tcctaaattc ataagagctt
     1561 tgttagcatt gtagcgtaaa aaggtcataa cttcttcagt ccaccctacg ccatcataaa
     1621 gcgttttggt gtatttttct tcattttcat acagctgata aaggaggtca tacatccaat
     1681 cacgaaaatt ctcttgctca tcttctggta gttcgttaaa accaagctgg aatttgtaac
     1741 cgatataagt accatgtaca gattcatcac gaataattag tttaatgatt tcagcaacat
     1801 ttgctagctt attatttccc aaatagtaaa gaggggtgaa aaagccagaa taaaaaagga
     1861 aagtttcgag atatgttgag gcaactttct tttgtaaggc atctccatta gcgtaaatgt
     1921 cattgataat acgtgctttt tcttgaagaa actcattatt attagtccac tcaaagatct
     1981 cttcaatttc tttttttgtg tttaaggttg aaaagataga cgaataagat ttagcgtgaa
     2041 ctgattccat gaattgaata ttgtttaaga cagcttcttc gtgaggagtg cgaacatcag
     2101 cacgaatagc ttcgacacca gtttctgatt gcatggtatc aagtagggtt aatcctccaa
     2161 aaaccttgcc aacaagatct ttttcttgaa gagaaagttt gcgccaatcg tctaagtcat
     2221 ttgataaagg gatacgggta tcaagccaaa attgttcggt caacttttcc caagttgact
     2281 tgtcaatgac gtcttcgatt tcattccagt taattgcttc ataatatgta gtcatagaaa
     2341 aatgatctcc taaagagtag taaagtgcgt taaggatatc gagataaacc agtagaaccg
     2401 accaaagttc taccggtaat aataagttta aatagattca aaagggtaac gaatgagtta
     2461 gtgagtggtt taggcagatc gcctaaagcg actgccgtag aagtaataga tgctttagca
     2521 tcattacttc ttaaccaatt aaatgacaca agattcacat tggtttgcgc ccacttcttc
     2581 cccatcatcc gtaaaggtac ggatatagta gatagattta atgcctttat tgaaagcgta
     2641 gtttcgtaag atggataaat cacgagtggt ttgtttgctt tgtgttttcc actcataaag
     2701 ctccataggc aactcactac gaaggaatag agttaatgac aagccttggt ccacatgttc
     2761 ggtcgcagcg gcatagacat caataacttt gcgcatgtcc atatcgtaag cagatgtata
     2821 gtaaggaatg gtatccgtag acaaaccatt tgcaggatag tagattttac caattttctt
     2881 ttcttgacgc tcttcgatgc gttgtgtgat tgggtgaata gaagcagagc agtcattgat
     2941 ataagaaata gagccatttg gagcaactgc tagtcggttt tgatgataaa gaccatcttt
     3001 ttgaacggcg tcgcgaagag cctcccaatc tgaagcttgc ggaataaaat ggtctttaaa
     3061 gagacttttg accaaatcag attttggaac aaagtgtcct gtaacgtatt tatcaaagta
     3121 actaccatca gcgtacttag agttctcaaa gccaacaaag gtagtttggc gctcacgagc
     3181 gatgttattg gattcgacca aggtccaata attcaggagc ataaagtaaa tatcagtaaa
     3241 ctcgatggat tctggactgc catattcaat atgatgttga gcaaggtaag aatgtagtcc
     3301 catagctcca agaccaaaag tatgtgcttg actgttacca tgtttaatgg ttgggacagc
     3361 ttcgatgttt gatgagtcgg tcacgaaggt taaggcacgt gtcatggtct taatagaacg
     3421 gccaaagtct ggtgaggtca tcatgttcag gatattagtg gaacctaagt tacatgaaat
     3481 atcagttccc atttctacaa actcttgcgc atcattgata aggctaggtg tttgaacttg
     3541 taaaatttca gaacacaagt tgctcatgat gatttttcca tcgataggat tagctttatt
     3601 agctgtatca atattgatga tataagggta accagattct tgttgtaatt ttgaaatctc
     3661 tgtttcaaga tcacgagctt taattttagt cttagtaatt ttagggttcg ccactaactc
     3721 atcgtacata ttggtaatgt cgagatagtt aaagggaatg ccatattctt tttcaacatt
     3781 gtaaggacta aagagataca tgtcctcgtt tttacgagct aattcgtaga atttatcagg
     3841 aacggtaatc cctagtgaca aggttttaac acgcaccttt tcatcggcat tttctttttt
     3901 agtagataag aaagcaatga tatcaggatg aaaaacattt aggtaaacaa caccagctcc
     3961 ttgacgttgc ccaagttgat ttgaataaga aaaactatct tcaaataatt tcataacagg
     4021 aacaactcct gaggctgcac cagcatagcc tttgattggt gcgccagctt cacggaggtt
     4081 agacaaggta atcccaacac ctccaccaat acgggataat tgcaaagcag agttgataga
     4141 acgtccgata gagttcatgt catcagttac ttgaatcaag aaacaagaga ccaattcacc
     4201 acgacggctt cgaccagcat ttaaaaagga aggagtagcc ggttggtaac gttggttaat
     4261 catttcaacg gctaaatctt ttgctaagtc ttcttgacca tctgcaaaat acaaagcatt
     4321 aaacaagaca cggtcttcaa ggttttctaa ataatgctct ccatcatttg tttttaaggc
     4381 gtattgctgg tagaacttgt aggctgccat aaatgattta aagcgaaaat tttctgattt
     4441 gattatgcta gctaattcag taataaattc agggtggtat ttctgaataa aagctgattc
     4501 aatgtaatca tttgagatta aatactcaat tttttccgta atggaagtaa aagacatggt
     4561 gtttggcagc acattttcag cgaaaaaagc ttttaaagct tctttgtctt tatgaagtgg
     4621 aattttacca ttaacaggac gattaatttc attatttagg cgaaaatatg aaatatcgcc
     4681 aagatctttg agactcatat tatttttcct tgtcttatta aatcaattct tttaactttg
     4741 caggttggaa gccagaaaat acgaggttat ctgcttcgat aacaggagca gatgtgaagc
     4801 caagactttt cacataatcc actttttcag gatgttcatc gatattaatt tcctggaagt
     4861 tggctccgtg ttgttctaaa aatttcttag tcattttgca ctgcatgcaa ttatttttgg
     4921 aatatactgt aatcatgtct tgtccttcta aaattagtaa tggttccatc ttaaatggaa
     4981 ttactataat agcatataaa aaataaagct aaaaagcaag ctataaactt atctgaaaca
     5041 caacatattg tgttgaaata aaaaacagaa acctaaatat tgtgtcaatc tggattctta
     5101 tggttagagc tctagcgttt tggtgataga agctaagcag ttttaataaa gcattaaagt
     5161 ctgaaaaata aattgtgaaa aacgtgaaaa taaaagaaaa taatttcaca ttttttgtcg
     5221 tttaaatatt taatcttgaa aataaaaaag cattgtgcta taatcggttg ttgtaagggt
     5281 tatcatgtga tggctcaaaa atacacaact taaaaggaga accatactat ggcttcaaaa
     5341 gactttcaca ttgttgcaga aacaggtatt catgcgcgtc cagcgacttt gcttgttcaa
     5401 acagctagca aatttgcttc agacatcact cttgactaca aaggtaaagc agtaaatcta
     5461 aaatcaatca tgggtgttat gagtcttggt gttggtcaag gtgcagatgt tactatctca
     5521 gctgaaggtg cagatgctga agatgccatt gcagcaattg aagaaacaat gactaaagag
     5581 ggattggcat aagactatga cagaaatgct taaaggaatt gcagcctcag acggcgttgc
     5641 tgttgctaaa gcatatctac tagttcagcc ggatttgtca tttgagactg ttacagtcgc
     5701 agatacaaat gcagaagaag ctcgccttga tgttgcactc caagctgcac aagacgagct
     5761 ttctgttatc cgtgaaaatg cagtagaaag cttaggtgaa gaagcagcag ccgtttttga
     5821 tgcccattta atggttcttg ctgatccaga aatgatcagc caggttaaag aaacgattcg
     5881 cgcaaaacaa acgaatgcag aaacaggtct taaagaagtg actgacatgt tcatcaccat
     5941 ctttgaaggc atggaagata acccatacat gcaagaacgt gcagcggaca tccgcgacgt
     6001 tgcaaagcgt gtgttggctc accttttagg tgtaaaactt ccaaatccag ctacaatcaa
     6061 tgaagaatca atcgttatcg cacacgattt gacaccttca gatactgctc aacttaacaa
     6121 acaatttgta aaagcatttg ttacaaatat cggtggtcgt acaagtcact cagctatcat
     6181 ggcacgtaca cttgagatcg ctgcggtact tggaacaaat gatattacaa aacgtgttaa
     6241 agatggtgat gtgattgccg ttaatggtat cactggtgaa gtgattatcg atccaagcga
     6301 agatcaagta cttgctttta aagaagctgg tgcggcttat gccaaacaaa aagcagagtg
     6361 gtctctcctt aaagatgcgc atactgagac agctgatggc aaacactttg aattggctgc
     6421 taatatcggt acgcctaaag acgttgaagg tgttaatgac aatggcgctg aagctgttgg
     6481 cctttaccgt actgagttct tgtacatgga ttctcaagac ttcccaactg aagacgaaca
     6541 atacgaagct tacaaggcag tgcttgaagg catgaatggc aaacctgttg tggttcgtac
     6601 gatggatatt ggtggcgaca aggaacttcc ttactttgac cttccaaaag aaatgaaccc
     6661 attcctcggt ttccgtgctc ttcgtatctc aatctctgaa actggggatg ccatgttccg
     6721 cacacaaatg cgtgcgcttc ttcgcgcctc tgttcacgga caacttcgta tcatgttccc
     6781 aatggtagca cttcttaaag aattccgtgc tgcaaaagca gtctttgacg aagaaaaagc
     6841 aaacttgctt gcagaaggcg ttgcggttgc tgatgacatc caagttggta tcatgattga
     6901 gattcctgca gctgctatgc ttgcagacca atttgctaag gaagttgatt tcttctcaat
     6961 tggaacaaac gaccttatcc aatacactat ggcagcagac cgtatgaacg aacaagtatc
     7021 atacctttac caaccataca acccatcaat attacgtttg atcaacaatg tgatcaaagc
     7081 agcgcacgct gaaggtaaat gggtaggtat gtgtggtgag atggcaggtg accaacaagc
     7141 tgttccactt cttgttggaa tgggcttgga tgagttttct atgtcagcaa cttcagtact
     7201 tcgtacgcgt agtttaatga agaaacttga ctctgctaag atggaagaat atgcaaatcg
     7261 tgcgcttaca gaatgttcaa cagcagaaga agttcttgaa ctttctaaag aatacgtttc
     7321 tgaagattaa cgggttaaag agttggttta aaccagctct tttttgctag ctaaaaatac
     7381 aattactttt tggtgtgaaa tcatttgagt tatcaaataa ttatcagaat tttcagatat
     7441 ttagtgtagc caaatcttga attatatcgg aaaagtgata gaataaaatt actatcataa
     7501 aaggagataa aacgttggca aaacaatata aaaatttagt gaacggtgaa tggaaactat
     7561 cagaaaacga gattaccatt tacgcaccag caacaggtga agagttagga tcagttccag
     7621 cgatgacgca ggcagaggta gatgctgttt acgcttcagc taaaaaggct ctatcagatt
     7681 ggcgcgcctt gtcttatgtg gaacgtgcag cttaccttca taaagcggct gatattttag
     7741 tacgtgatgc tgaaaagatc ggcgcgattc tttcaaaaga agtagccaaa ggtcacaagg
     7801 cagctgtcag tgaagttatt cgtaccgctg aaatcattaa ttatgcagca gaagaagggc
     7861 ttcgtatgga aggtgaagtt cttgaaggtg gtagcttcga agccgcaagt aagaagaaga
     7921 ttgctattgt tcgtcgtgaa ccagttggtt tagttcttgc catctcacct tttaattatc
     7981 ccgttaactt ggcaggttct aaaattgctc cagcccttat ttcaggaaat gttgttgctc
     8041 ttaaaccacc aacacaaggc tctatttctg gtttgttact agcagaagct tttgcagaag
     8101 ctggtattcc agcaggtgtc tttaatacca ttacagggcg aggttctgtt attggggatt
     8161 acatcgttga gcatgaagcg gttaacttta tcaactttac aggttctact ccaattgggg
     8221 aaggaatcgg taaattagcg ggtatgcgac caattatgct tgagcttggc ggtaaggatt
     8281 ctgctatcgt tttggaagat gcagatcttg ttttagcagc gaaaaatatt gtagccggtg
     8341 cttttggtta ctcaggacaa cgttgtacag cggttaaacg cgttcttgtg atggacaagg
     8401 tggcggatca attggcggct gagattaaaa cacttgttga aaaactaagt gtcggaatgc
     8461 ctgaagccga tgctgatatt acaccattaa ttgatacatc agctgctgat tttgttgaag
     8521 ggttgattaa agatgcaact gataagggag ctactgcttt gacagccttt aatcgtgaag
     8581 gcaatcttat ttcacccgtt ctctttgatc atgtgacaac tgacatgcgt ttggcatggg
     8641 aagagccgtt cggcccagta ttaccaatta ttcgtgtaac cactgtagaa gaagccatca
     8701 agatttctaa tgagtctgaa tatggtttgc aagcttctat ttttacaact aatttcccaa
     8761 aagcttttgg cattgctgag caattagaag ttggaactgt tcaccttaac aataaaacac
     8821 aacgtggaac agataatttc ccattcttag gcgctaaaaa atcaggtgca ggggtacaag
     8881 gagttaaata ttctatcgaa gctatgacaa ctgttaaatc tgttgtattt gatatccagt
     8941 aataaaaaaa ataaacaaaa agttaggtta acttaacttt ttgtttattt tt
//
LOCUS       DAUCLR010000077         8987 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.077, whole genome shotgun sequence.
ACCESSION   DAUCLR010000077 DAUCLR010000000
VERSION     DAUCLR010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8987
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.077"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            167..934
                     /gene="rpsB"
                     /locus_tag="VGT87_001476"
     CDS             167..934
                     /gene="rpsB"
                     /locus_tag="VGT87_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEQ3814313.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKLADQAYEFVRDAAANDAVILFVGTKKQAAEAVADEATRAGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEKGTFDVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNADPDDIDIIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDADVAFEADTQADSIEEIVEVVEGDNA"
     gene            1068..2108
                     /locus_tag="VGT87_001477"
     CDS             1068..2108
                     /locus_tag="VGT87_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEQ3814314.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDMDKAVELLREK
                     GMAKAAKKADRVAAEGLTGVYVHGNVAAVVEVNAETDFVAKNAQFVELVNATAKVIAE
                     GKPANNDEALALVMPSGETLAEAYVNATATIGEKISFRRFALIEKTDEQHFGAYQHNG
                     GRIGVISVVEGGDDALAKQVSMHIAAMKPTVLSYTELDAQFIKDELAQLNHAIELDNE
                     SRAMVDKPALPFLKYGSKAQLSDDVITAAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLDSVNAKAIAFARFEVGEGIEKKANDFES
                     EVAATMAAALNN"
     gene            complement(2275..4170)
                     /gene="pepO"
                     /locus_tag="VGT87_001478"
     CDS             complement(2275..4170)
                     /gene="pepO"
                     /locus_tag="VGT87_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEQ3814315.1"
                     /translation="MTTYQDDFYQAVNGKWAETAVIPDDKPRTGGFSDLADEIEALML
                     DTTDAWLAGENIPDDAILKNFVKFHRLVADYAKRDEVGVSPILPLIEEYQSLKSFSEF
                     VANIAKYELAGLPNEFPFSVAPDFMNAQLNVLWAEAPSILLPDTTYYEEGNEKAEELR
                     GIWRQSQEKLLPQFGFSTEEIKDLLDKVIELDKQLAKYVLSREEGSEYAKLYHPYVWA
                     DFKKLAPELPLDSIFEKILGQVPDKVIVPEERFWTEFAATYYSEANWDLLKANLIVDA
                     ANAYNAYLTDDIRVESGAYSRALSGTPQAMDKQKAAFYLAQGPFSQALGLWYAGQKFS
                     PEAKADVESKVARMIEVYKSRLETADWLAPATREKAITKLNVITPHIGYPEKLPETYA
                     KKVIDESLSLVENAQNLAKITIAHTWSKWNKPVDRSEWHMPAHLVNAYYDPQQNQIVF
                     PAAILQEPFYSLDQSSSANYGGIGAVIAHEISHAFDTNGASFDEHGSLNDWWTQEDYA
                     AFKERTDKIVAQFDGLESHGAKVNGKLTVSENVADLGGVACALEAAQSEEDFSARDFF
                     INFATIWRMKAREEYMQMLASIDVHAPGELRTNVTLTNFDAFHETFDIKEGDAMWRAP
                     KDRVIIW"
     gene            complement(4378..6006)
                     /gene="treC"
                     /locus_tag="VGT87_001479"
     CDS             complement(4378..6006)
                     /gene="treC"
                     /locus_tag="VGT87_001479"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEQ3814316.1"
                     /translation="MTIDKKKVVYQIYPKSYKDTTGNGVGDLRGIIDKLPYLQELGID
                     MIWLNPFYPSPQRDNGYDVSDYTAVNPDFGTMADFEDLVKAAKEHQIELMLDMVLNHC
                     STDHEWFQKALAGDPYYQDFFILRDQPTDWVSKFGGNAWAPFGDTGKYYLHLFDVTQA
                     DLNWRNPHVREELAKVVNFWRDKGVKGFRFDVINLIGKDEELVDCPVNDGKPAYTDRP
                     ITHTYLHDLNQASFGQDDSFMTVGEMSATTIDNCLLYTAPEREELSMAFNFHHLKVDY
                     ENGQKWTIMAFDFAALRDLFHAWGEGMSQGNGWNALFYNNHDQPRALNRFVDVTHFRN
                     EGATMLAASIHLSRGTPYIYMGEEIGMLDPDFDSMDDYVDVESLNAYSSLLVSGKSAE
                     EAFAIIKDKSRDNARTPMQWDASEHAGFTTGKPWLEVSKSYRDINVETEKEGRIFPFY
                     QRLIALRKELPIIAEGDYRAAFKDSQSVYAFERHLGDQCLLVLNHFYADEVELELPPR
                     YQHGQVLISNYEKVSICEKVILKPYQTLAILAEN"
     gene            complement(6074..8098)
                     /gene="treP"
                     /locus_tag="VGT87_001480"
     CDS             complement(6074..8098)
                     /gene="treP"
                     /locus_tag="VGT87_001480"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEQ3814317.1"
                     /translation="MGKFEQDAKSLLTAIGGKENIKAVTHCATRMRFVLNDNNKANVK
                     EIEKISVVKGTFTNAGQFQVIIGNDVPVFYNDFTAVSGIEGVSKEAAKSAAKSNQNAL
                     QRVMTMLAEIFTPIIPAIIVGGLILGFRNILESVPFEFLGQQVEKGKLVFDAAGDPVW
                     NTIVRVSPFWSGVNHFLWLPGEAIFHFLPVGITWSVTRKMGTTQILGIVLGICLVSPQ
                     LLNAYAVAGTPAAEIAKNWVWDFGFFTINRIGYQAQVIPALLAGLSLAYLETFWRKRI
                     PEVVSMIFVPFLSLIPALILAHTVLGPIGWTIGKGISFVVLAGLTGPVKWLFGAIFGA
                     LYAPLVITGLHHMTNAIDTQLIADTATRTTGLWPMIALSNIAQGSAVFAYYLMNRHEE
                     REAEISLPAAISAYLGVTEPALFGVNVKYVYPFVAGMIGSGIAGLLSTTFNVQANSIG
                     VGGLPGFMAINVKYMIPFFICMAVAIVVPMFLTFFFRKSHIMTKTEDEAKLPETPVSE
                     APAATAPHKTMQGTVITLTSPLTGEVKALSEAVDPVFAQGVMGQGALLQPTEGVLVAP
                     CDAEVSVLFPTKHAICLVTTEGLELLMHIGMDTVNLDGQGFEALVKQGDQVKAGQTLI
                     QFDIAAISEAGYATETPLVVTNQDAFTVTVEGSLPRQIKANDKLAVAMKK"
     gene            8309..>8987
                     /gene="treR"
                     /locus_tag="VGT87_001481"
     CDS             8309..>8987
                     /gene="treR"
                     /locus_tag="VGT87_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEQ3814318.1"
                     /translation="MTKYERIYKDLETKINKDFYKEGDFLPTEIELSQQYQASRDTVR
                     KALSLLTKAGLILKKQGRGTQVIKHHQIMFPISELTSYQELVSYSNLDSKTNVIAIDK
                     LIVDETLSKLTGFSKNSLVWRVTRQRVVEGVASVLDIDYLSKTLVPIMTREIAEHSIY
                     QYLEKELHLAIDFALKEVTIDQITDRDKILLDLGSDQHVVSVKSKVYLSNNNQFQFTE
                     SRHKLEKF"
BASE COUNT         2810 a         2010 c         1658 g         2509 t
ORIGIN      
        1 ttcatagcat ttctccttgc ttttaacctc tttttttgat aatatagtag ggtcgtgtag
       61 acggcaaata ctcattctag accaattgtg ggcttgttgc cgctaggtta gcctgcaagt
      121 cgaagtctgg aagaggaaaa aacattttat aaaggagaaa ctactcatgg cagtaatttc
      181 aatgaaacaa cttcttgagg ctggtgttca ctttggtcac caaactcgtc gctggaaccc
      241 taagatggct aaatacatct tcacagagcg taacggtatc cacgttatcg atcttcaaca
      301 aactgtaaaa ttagctgacc aagcttacga atttgttcgt gatgctgcag caaacgatgc
      361 tgtcatcttg ttcgttggta ctaaaaaaca agctgctgaa gcagttgctg acgaagcaac
      421 gcgtgcaggc caatacttca tcaaccaccg ttggttgggt ggaacgctta ccaactgggg
      481 aactatccaa aaacgtatcg ctcgtttgaa agaaatcaaa cgtatggaag aaaaaggaac
      541 ctttgacgtt cttcctaaga aagaagttgc acttcttaac aaacaacgcg ctcgtcttga
      601 aaaattcttg ggcggtatcg aagatatgcc tcgtatccca gacgtgatgt atgttgttga
      661 cccacataaa gaacaaattg ctgttaaaga agctaaaaaa cttggtatcc cagtcgtagc
      721 gatggttgat acaaacgctg atccagatga tatcgatatc atcattccag ctaacgatga
      781 cgctatccgc gccgttaaat taatcactgc taaattagct gacgctatta tcgaaggtcg
      841 tcaaggtgaa gatgcagacg ttgcttttga agctgacact caagcagatt caatcgaaga
      901 aatcgttgaa gttgtcgaag gcgacaacgc ttaattggca cagagttcct aactcttatg
      961 gcaaactttg tttgtcttta acaatcgaaa accaaacggg gcagaaagca aaccgctctg
     1021 tctcgttttt tgaaaccaaa acaaatatca tttggaggat actacacatg gcagaaatta
     1081 cagctaagct tgtaaaagaa ttacgtgaaa aatctggtgc tggcgttatg gacgctaaaa
     1141 aagcacttgt tgaaacagat ggtgacatgg acaaagccgt tgaacttctt cgtgaaaaag
     1201 gaatggctaa agcagctaaa aaagctgacc gtgttgccgc tgaaggttta acaggcgttt
     1261 acgttcatgg taacgttgct gctgttgttg aagttaacgc tgaaacagac tttgttgcta
     1321 aaaatgctca attcgttgaa ttggtaaacg caactgctaa agttatcgct gaaggcaaac
     1381 cagctaacaa cgatgaagca cttgcacttg ttatgccttc aggtgaaact cttgctgaag
     1441 cttacgttaa tgcaactgct accatcggag aaaaaatctc attccgtcgt tttgctttga
     1501 ttgaaaaaac agatgagcaa cacttcggtg cttaccaaca taatggtggc cgtatcgggg
     1561 ttatctcagt tgtcgaaggc ggcgacgatg ctcttgctaa acaagtatca atgcatatcg
     1621 ctgcaatgaa accaactgtt ctttcataca ctgagcttga tgcacaattc atcaaagatg
     1681 aacttgctca attgaaccac gctatcgaac ttgacaacga atcgcgtgca atggttgaca
     1741 aaccagcact tccattcttg aaatacggtt caaaagctca attatcagac gacgtgatta
     1801 ctgctgctga agcagacatc aaagctgaac ttgctgctga aggcaaacca gaaaagatct
     1861 gggacaaaat catcccaggt aaaatggatc gtttcatgct tgacaacact aaagttgacc
     1921 aagcttacac tcttcttgca caagtttata tcatggacga cagcaaaact gttgaagctt
     1981 accttgactc agtaaacgct aaagcgattg cttttgcacg ttttgaagtt ggtgaaggta
     2041 tcgagaaaaa agctaacgac tttgaatcag aagttgcagc tactatggct gctgccctta
     2101 acaactaatc acttatttct gataagtcta aaaaaggcaa ccttctttcc tgagggttgt
     2161 ctttttgttg tgtcaaaacg gacaccaata aggaagcaaa aaactgatcc tgtgtttcac
     2221 agaatcagtc atttttataa tcacaaagca tttgaaggtt tatcaactac ctaattacca
     2281 aataatcaca cggtctttcg gagctctcca catagcatcg ccttctttaa tatcaaaggt
     2341 ctcatggaaa gcatcaaagt tggttaaggt gacatttgtt cgcagttcac ccggagcatg
     2401 aacatcaatg ctagctagca tttgcatgta ttcctcacgg gccttcatac gccaaatcgt
     2461 cgcaaagtta ataaagaagt cacgtgctga gaagtcttct tctgattgtg ccgcttctaa
     2521 agcacaagct accccaccta gatcagcgac attttctgaa actgttaatt taccgttgac
     2581 cttagcacca tgtgactcta agccgtcaaa ctgcgccaca atcttatctg tacgctcttt
     2641 aaaggcagca tagtcttctt gtgtccacca atcatttagg ctaccatgtt catcaaagga
     2701 agcaccattg gtgtcaaagg catgtgaaat ctcatgcgca atgactgcac caattcctcc
     2761 ataattggct gaagaactct gatctaaaga ataaaatggc tcttgtaaga ttgccgcagg
     2821 aaagacaatc tgattttgct gaggatcata ataagcattg actaagtggg ctggcatatg
     2881 ccactcacta cggtcaactg gcttgttcca tttactccaa gtgtgagcaa tcgtgatttt
     2941 agccaagttc tgtgcatttt caaccaatga caagctctca tcaatcactt ttttagcata
     3001 ggtttctggt aatttttctg ggtaaccaat atgtggcgtg ataacattta atttagtaat
     3061 ggctttttca cgtgtggctg gtgctaacca atcagccgtt tctagacgag acttataaac
     3121 ctcaatcatg cgtgctacct tactttccac atcagcctta gcttctgggg agaatttttg
     3181 accagcatac cacaagccaa gcgcttggct aaacggtcct tgcgccaaat aaaaggctgc
     3241 tttttgtttg tccatagctt gaggtgttcc tgaaagagcg cgcgaataag cgcctgattc
     3301 cacacgaata tcatccgtca agtaagcatt gtaagcatta gccgcgtcca caatcaaatt
     3361 agccttgagt aaatcccaat tggcttcaga gtaataagtg gctgcaaatt ctgtccaaaa
     3421 acgttcctca ggaacaataa ccttgtcagg tacttggcct aaaatctttt caaaaatgct
     3481 atccaaagga agctctggag ctagtttttt aaaatcagcc cacacataag gatggtataa
     3541 tttggcatat tctgagcctt cctcacgaga caagacatac ttggccaatt gcttatctag
     3601 ctcaattacc ttatccaaaa gatccttgat ttcttctgtt gaaaaaccaa attgaggtaa
     3661 aagcttttct tgcgactgac gccaaatacc acgcaattcc tcagcttttt cgttgccttc
     3721 ttcgtagtat gttgtatctg gcaaaagaat gcttggtgct tctgcccaaa gcacattcag
     3781 ctgagcattc ataaagtctg gtgccacact aaagggaaat tcatttggta aacctgccaa
     3841 ttcatatttc gctatattag caacaaattc tgaaaaagat tttaaggact gatattcttc
     3901 aattaaaggg agaattggac ttactccaac ttcatcacgt ttagcgtagt cagccaccaa
     3961 acgatggaat ttcacaaaat tttttaaaat agcatcatca gggatatttt ctccagctag
     4021 ccaagcatct gtagtgtcta gcattaatgc ctcaatttca tctgctaagt ctgaaaatcc
     4081 acctgttcga ggcttgtcgt ctggaataac tgctgtttca gcccactttc cattcacagc
     4141 ctggtaaaaa tcatcttgat aagttgtcat tcgtatctcc ttatatcatt ttaaatttgt
     4201 agaatcttta tcttaaactc tacttataat cttttccatt gtaccaaaat ttagctaaaa
     4261 taacttatct caattgtttt tatttagaaa aaataagagt ttaaaaataa atatatgaga
     4321 aaacttcttt tttcgtgaca atcaccaaaa gcctgaggcc taacctcagg cttttagtta
     4381 gttttcagct aagatagcaa gtgtctgata aggtttcagt atcacttttt cacaaataga
     4441 aactttctca tagttgctga ttaagacctg tccatgttga taacgtggag gtaattccag
     4501 ttcgacctca tcagcataga aatgattgag aacaagcaaa cactggtcac ctaaatggcg
     4561 ttcaaaggcg tagacagact gactatcttt aaaagcagcc cgataatccc cttcagcaat
     4621 aatagggagt tccttccgca aagcaatcaa gcgttggtag aaaggaaaaa tacgtccctc
     4681 tttttctgtt tcgacattga tgtctcgata agatttgcta acctctaacc aaggcttacc
     4741 agtcgtaaag ccagcatgtt cactagcatc ccattgcatt ggtgttctgg cattgtccct
     4801 tgacttatcc ttgataatgg caaaggcttc ttctgcactt ttacctgaga ctagtaagct
     4861 tgagtaagca ttgagacttt ccacatccac ataatcatcc atactatcaa agtctggatc
     4921 aagcatgcca atctcctcac ccatataaat gtaaggcgtt cctcgtgaca aatggatgga
     4981 agccgctaac atcgtcgcac cttcgtttcg gaaatgtgtt acatcaacaa aacgattcag
     5041 ggcacgtggt tgatcatggt tattgtagaa caaggcattc cacccattgc cttgactcat
     5101 gccttcaccc caagcatgga ataagtctcg cagcgctgca aaatcaaaag ccataatagt
     5161 ccatttctga ccgttctcat aatcaacttt tagatggtgg aaattaaaag ccatggatag
     5221 ctcctcacgt tcgggagccg tgtataaaag acagttgtca atagtcgtgg cagacatttc
     5281 ccctactgtc ataaacgaat catcctgacc aaaactggct tgattgagat catgaagata
     5341 agtgtgagta ataggacgat ccgtataagc tggcttacca tcattgactg gacaatccac
     5401 cagctcttca tctttcccaa tcagattaat cacatcaaac cggaacccct tcactccttt
     5461 atctcgccaa aaattaacca ctttagccaa ttcctcacga acatgtgggt tccgccaatt
     5521 caagtcagcc tgtgtcacat caaacaagtg taagtagtat ttgcctgtat ctccaaaagg
     5581 cgcccaagca ttcccaccaa atttggaaac ccaatcagtc ggctgatctc tcaagataaa
     5641 gaaatcctga taataagggt ctcctgctaa agctttttgg aaccactcgt ggtctgtgga
     5701 acagtgattc aaaaccatat ccaacattaa ctcaatctga tgctccttag cagcttttac
     5761 caaatcttca aaatcagcca tagtcccaaa atcagggtta accgccgtat aatctgaaac
     5821 atcatagcca ttgtctcgtt gtggactagg gtagaaaggg ttcaaccaaa tcatatctat
     5881 tcctagttct tgtaagtaag gcaatttatc aatgatccct cgcaagtctc ccacaccatt
     5941 tccagtcgtg tccttatagg attttgggta aatttgatag acgactttct ttttatcaat
     6001 tgtcatgagt tttcctccct aaaatagttt caggaactga tctcagttcc tgaacgtttt
     6061 ctccctagta aacctacttt ttcatcgcta ctgctaactt atcattagcc ttaatctgac
     6121 gcggtaaact accttcaaca gttaccgtaa aagcatcttg attagtcacc acaagaggcg
     6181 tttcagtggc gtatccagct tcagaaattg ctgctatatc aaattgaatc aatgtttgtc
     6241 cagccttaac ctgatcacct tgcttcacca aagcttcaaa tccttgacca tctaagttaa
     6301 ccgtgtccat gccaatatgc atcaataatt ccaaaccttc agtcgtcacc aaacaaatag
     6361 cgtgtttagt tgggaacaag accgatactt cagcatcaca aggcgctact aacacccctt
     6421 ctgtcggttg aagaagagca ccttgaccca taactccctg tgcaaagaca ggatcaacag
     6481 cttcagacaa cgctttaact tcacccgtta aagggcttgt taaagtgata actgtccctt
     6541 gcatagtctt atgtggagca gttgctgcag gagcctccga aacgggtgtc tcaggtagtt
     6601 tagcctcatc ttctgtctta gtcatgatat gtgatttacg gaaaaagaag gttaaaaaca
     6661 tcggtaccac aatggctact gccatacaga tgaagaatgg aatcatatac ttcacattga
     6721 tagccatgaa acctggtaag ccaccaacac caatagaatt tgcctgaaca ttaaaggttg
     6781 ttgataagag acccgcaata cctgagccaa tcattccggc tacaaaggga taaacatatt
     6841 taacattaac cccaaataag gcaggctcag ttaccccaag gtaagcagaa attgctgcag
     6901 gaagcgatat ttcagcctca cgttcttcat gacgattcat taaatagtaa gcaaaaacgg
     6961 ctgacccttg agcgatattt gaaagagcaa tcattggcca caaaccagtt gtacgagttg
     7021 cagtatcagc aattaattgg gtatcaatgg catttgtcat gtgatgtaaa ccagtaataa
     7081 ctagcggagc atacaaggca ccaaagatag caccgaatag ccatttaaca ggaccagtca
     7141 atccagctaa cacaacaaag gaaatccctt taccaatagt ccaaccgatt ggccccaata
     7201 ccgtatgcgc taaaatcaaa gctggaatca aagaaaggaa tggcacaaaa atcattgaaa
     7261 ccacttctgg aatccgttta cgccagaaag tttcaagata agcaagggac agaccagcta
     7321 aaagggctgg aataacctgt gcctgatacc caatacgatt aatggtaaag aaaccaaaat
     7381 cccaaaccca gtttttggca atctcagcag caggcgttcc tgccaccgca taagcattca
     7441 ataattgtgg tgacaccaaa cagataccaa ggacaatccc taaaatttga gtggttccca
     7501 tcttacgcgt cacagaccaa gtaatcccaa ctggtaagaa gtgaaaaata gcttcccctg
     7561 gtaaccacaa gaaatggtta acccctgacc agaaaggaga taccctcaca atcgtattcc
     7621 aaacaggatc cccagctgca tcaaaaacta atttcccttt ttcgacctgc tgcccaagaa
     7681 attcaaaagg cacactctcc aaaatattac ggaaacctaa aataagcccc ccaacaataa
     7741 tcgccggaat aataggtgtg aaaatctcag ccaacatggt catcacccgt tgtaaggcat
     7801 tttgattact tttagctgct gatttggcag cttctttaga caccccttca ataccagaaa
     7861 cagctgtaaa gtcattataa aaaactggaa catcattacc aatgattacc tgaaactgcc
     7921 cagcattggt aaatgtccct ttaactacag agattttttc aatctctttg acatttgcct
     7981 tattattatc attcaaaaca aaacgcatac gcgttgcaca gtgtgtaaca gccttgatgt
     8041 tttctttacc gccaatagca gttagaagac ttttagcatc ctgttcaaat tttcccatgt
     8101 gacatcccct agtcctttgt tctagttgct tttaacaagc aacgtcaaaa tgaaatattc
     8161 aaattcttag ataaaatatg attgacacct ttattgtaat cgattgcaaa tttgtctgca
     8221 agttgtttgt gccaaaaaag ccattatttt ggtaaaataa aaggtgttgt ctgtaaaaaa
     8281 atagacaaat ggaaaggtaa tttaccctat gacaaagtat gaacgtattt ataaagacct
     8341 cgaaacaaaa ataaacaaag acttttacaa agaaggggat tttttaccta ctgaaataga
     8401 attgagtcaa caataccaag ctagccgaga taccgtccgc aaggctctct cacttctcac
     8461 aaaggcaggt cttatcctca aaaaacaagg acgtggcacc caagtgatta aacatcatca
     8521 gatcatgttc cctatctcag aactaactag ttatcaggaa ctcgtctctt actcaaatct
     8581 agattctaag accaacgtca ttgccattga taaactgatt gtggatgaga cactttcaaa
     8641 attgaccggt tttagcaaaa acagcttggt ctggcgtgtt acacgccaac gcgttgttga
     8701 gggtgtggct tccgtcttag acattgatta ccttagtaaa actttggtgc caataatgac
     8761 aagggaaatc gcagagcatt ctatttatca atatttggaa aaggaactcc atctcgccat
     8821 tgactttgcc ctcaaagagg ttaccattga ccaaataacc gatcgtgata aaattttact
     8881 tgaccttgga tcagaccaac atgttgtttc cgtcaaatcc aaagtctacc tttccaataa
     8941 taaccaattc caattcaccg agagtcgcca taaactggaa aaattta
//
LOCUS       DAUCLR010000078         8519 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.078, whole genome shotgun sequence.
ACCESSION   DAUCLR010000078 DAUCLR010000000
VERSION     DAUCLR010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8519
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.078"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            28..189
                     /gene="sagA"
                     /locus_tag="VGT87_001482"
     CDS             28..189
                     /gene="sagA"
                     /locus_tag="VGT87_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOMM family cytolysin streptolysin S"
                     /protein_id="HEQ3814319.1"
                     /translation="MLKFTSNILATSVAETTQVAPGGCCCCCTTCCFSIATGSGNSQG
                     GSGSYTPGK"
     gene            411..1361
                     /gene="sagB"
                     /locus_tag="VGT87_001483"
     CDS             411..1361
                     /gene="sagB"
                     /locus_tag="VGT87_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptolysin S biosynthesis dehydrogenase SagB"
                     /protein_id="HEQ3814320.1"
                     /translation="MSFFTKEQQPKENCPPITVEKARQLFEFNTNHLSLSDYHHQTVL
                     KTSKQLVAQHLMPNATDNLSQHFLMNYKANNNYLGFQASIVDFFTDSAVANFSSSYVY
                     ESQEKIIRLPKPTKISTALSTCIIKRRSHRQFSDRQMPLQDLSNILYYACGVSSQASI
                     RDGASDKITLRNCASGGGLYPIHLVFYARNISKLIDGFYEYLPYQHALRCYRHSSEEN
                     VRDFAEYGAINAENCNIIIIYVYHYMKNTRKYGNQATAYAFIESGEIAQNIQLTATAL
                     TYGSIDIGGYNKEYLQELLDLDGLGEHVIHMTLVGTKESQ"
     gene            1358..2416
                     /locus_tag="VGT87_001484"
     CDS             1358..2416
                     /locus_tag="VGT87_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptolysin associated protein SagC"
                     /protein_id="HEQ3814321.1"
                     /translation="MKYQLNSNVRVVTFQDTFCFRKGIWDFNEAILDLTQEPQNLKIV
                     YQEIVSQLVKGVAIDTETYENTLEPETFANLMEVISGLYYNDMLMLEDDYNLEENVMK
                     ILMGNFRFMAQAGHMVSNAPVLFISDSTYVNESAELLAEQLHLQLQVASDDLKMLIQQ
                     TDVSSRLDALEHRRNMNCLSEALCDYQSIIICQERLNIMMLRHLNEVSVAMKKQLVIG
                     FVDGPFLHTCTLNPPHSADFDSLERRVLARLQDSTLYQHFANQVLPATQDVSQAYLPL
                     LNVLMNLVVSEAFIIVQTGSSKFEGRLLSIYLPTLEIQVQDILKMSNSQTQGALAKLK
                     YEDQQISTREIVKKLLDE"
     gene            2436..3794
                     /locus_tag="VGT87_001485"
     CDS             2436..3794
                     /locus_tag="VGT87_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HEQ3814322.1"
                     /translation="MLYYYPSFNHIFDELKSLSGNRTGILNQSQVPVCNHPHDVYLKS
                     ITGQMPDYHKQFIGELSQVSYHIIGYGSYYEEALIKYLGESIERYATVIAGDLLSDRI
                     VYASYNELKLLHKVMPLEYLQVFTQEQIALSCDLQMMMCDKMVTENDVLGWVKCPMFF
                     EDAEMYVPAQMLCVGYKTNETVGERRIIPGFSTGTASHKTLEAAMCNSLIEYIQIDSM
                     MLSWYTKKPCPKIIVDDPDIEVILEEARLGKDSLYDIIPIDMTVGEDNPLYTFGIILK
                     NKYDEGPYLLFGVQAGLDPKHALLRGIMEASAISYSYYYNLLYQKASLANIECEEPLF
                     LDLDSNVFYYAHPKDQDHKWKAFEPLISGEVLLSDLEDHSGKDKKEDLKTLLAYAKKV
                     SPNAVFLDITPPEALEKGWYVTRVLMPELLEMCIPAFPFANHPRMRQFGGVTNAFVHP
                     MP"
     gene            3769..4440
                     /locus_tag="VGT87_001486"
     CDS             3769..4440
                     /locus_tag="VGT87_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEQ3814323.1"
                     /translation="MPLSIQCLNLCFLLVTFCPSIPMQAIFGKEDSGYAFNLIGFLRA
                     TLIYDILALVSIYVLSPKITLSLESIDSKTFFMGLVFCVFIVLIELVFLHGLRCWQKK
                     EWLPATFSFVGTTNDWSKIGYPLLLALFEETIYRFLWFNILAFQWHLPTIIVLIVTSF
                     CYALNHLLMGKSIFYAKLVTGIIYGSIYMLTSQLWLVVIMHVGGNLLVECLSHLQTKK
                     KKEVT"
     gene            4437..5120
                     /locus_tag="VGT87_001487"
     CDS             4437..5120
                     /locus_tag="VGT87_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptolysin associated protein SagF"
                     /protein_id="HEQ3814324.1"
                     /translation="MMLLVLLSFLGLTTLWLTSYRRRCLARWHLQWLMALSYQDFLDV
                     LLSLFQFVVIILVLFFYSATINLGEVLTFLTQTSWHWQILCYLVLYLMAIIEMTLLVL
                     ILIFDVLLQKDSRLLFKKITWLPFRQDKPVLSILLLGIVILVDSLFYLGLLILLGEQS
                     LTSLATLILGYGIVKACRYSGWLNVLLAFCLFTIVGLWAVTATLLYGWLVGAMILTVT
                     YLMISCKEY"
     gene            5143..6066
                     /locus_tag="VGT87_001488"
     CDS             5143..6066
                     /locus_tag="VGT87_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ3814325.1"
                     /translation="MSFVQLTNVVKSYKNGKKAVNDVSLSIEAGNIYGLLGPNGAGKS
                     TLINLILGLIPLSSGKITVLGQSQKTIRKISSQIGYVPQDIAVYPDLTAYENVELFGS
                     LYGLKGAQLKKQVLKSLEFVGLHSQAKQFPSQFSGGMKRRLNIACALVHSPKLIIFDE
                     PTVGIDPQSRNHILESIRLLNKEGATVIYTTHYMEEVEALCDYIFIMDHGQVIEEGPK
                     FELEKRYVANLANQIIVTLTDSRHLELADKPDWSLIEDGEKLMLKIDNSDMTSVVHQL
                     TQANITFSEIRHNHLNLEEIFLHLTGKKLRD"
     gene            6075..7202
                     /locus_tag="VGT87_001489"
     CDS             6075..7202
                     /locus_tag="VGT87_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ3814326.1"
                     /translation="MVLFHLIKKESLQIFRNRTALLMMVIFPILMIVILSFAFKSSFN
                     TATTVPKLTIRYQLEGEKTDYQKNFLAFLKVLNQKLHLEAKPSNSLEKDRQRVSEGAL
                     TAVLEVKKNQTIKVITNNINQQNADLINMLVKNYVDNAKTYDSIAALYPQQLNHIRKR
                     SVDYVKVSSIQTSKGMTSADYYAISMFTMITFYSMMSAMNLVLSDRQQRITNRIRLTG
                     VSPSLLVFGKLIGAMLATTVQLSLLYIFTRFVLRVNWGTNEWMLIGITASLVYLSVAI
                     GIGLGISIKNEAFLTVASNTIIPIFAFLGGSYVPLTTLHSSIINQLSNISPIKWVNDS
                     LFYLIFGGQYNPIPVTLIVNISIGTIFIILALIGMRKQVTT"
     gene            7199..8317
                     /locus_tag="VGT87_001490"
     CDS             7199..8317
                     /locus_tag="VGT87_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ3814327.1"
                     /translation="MICFIKTLFVKIKRKKTSYVTFFLMPILTTLLALSLSFSNNNQA
                     KIGILDKDNSQISKQFIAQLKQNKKYDIFTKIKKEHIDHYLQDKSLEAVLTIDKGFSD
                     KVLQGKSQKLNIRSIANSEITEWVKAQTNYLLENYNIIGDVALGNEDTFNRILQKNQQ
                     LNYDVKQVTLTDRSRSKAVSSTTTGFLLILMLGSTSVIYSGILADKSSQLYHRLMLSN
                     LSRFRYMLSYVCVGFVAFTIQIVIMLSLLKVFNISFFVPTSLLLIIFFLFSLLAIGFG
                     LLIGAITQNSQQSSQLANLIVMPTSMLAGCLWPLSITPSYMQAIGKLLPQNWVLSAIA
                     IFQSGGTLSQAWPYLLALMGTALALIIFSSLLLKPTRL"
BASE COUNT         2653 a         1317 c         1618 g         2931 t
ORIGIN      
        1 tacttattag ataaggaggt aaaccttatg ttaaaattta cttcaaatat tttagctact
       61 agtgtagctg aaacaactca agttgctcct ggaggctgct gttgctgctg tactacttgt
      121 tgcttctcaa ttgctactgg aagtggtaat tctcaaggtg gtagcggaag ttatacgcca
      181 ggtaaataat ctatttagca tctctatgtg atagtgatat taaggtaatg agtttgctag
      241 caactaagtt atctttggta acttctgatt atagatgaat ggcatagagg tgttagaaaa
      301 catgagacaa aagtaatctt cttgatgcta acgtctcagg taattagcag gtactagaca
      361 gtacctgcta attactatat gtttagtaaa atgagatagg aaagatagtt atgtcatttt
      421 ttacaaagga acaacaacct aaagagaatt gtccgccaat aactgttgaa aaagcaaggc
      481 aattgtttga atttaataca aaccacttgt ccttatcgga ttaccatcat caaacggtgc
      541 taaaaacgtc aaagcagcta gttgctcaac atttaatgcc taatgcaacc gataatctta
      601 gtcaacattt tttgatgaac tataaagcta ataataatta tttaggcttc caagctagta
      661 ttgtcgactt ttttacagat tctgccgttg ctaatttttc aagtagttac gtttatgaaa
      721 gtcaggaaaa gataattcgt ttaccaaaac ctaccaagat atcaactgct ctgtcgacat
      781 gtattataaa acgaagaagt catcgtcaat tttcagatag acaaatgcct cttcaagatt
      841 tatcaaacat tctttattat gcatgtggtg ttagttcaca agcatcaatt agagatggag
      901 catcagataa gattacactc agaaactgtg cttcaggtgg aggtttatac cctattcatt
      961 tagtttttta tgctagaaac atcagtaaat taatagatgg tttctatgaa tatctaccct
     1021 atcagcatgc actaaggtgt tatcggcata gctctgagga aaacgttaga gattttgcgg
     1081 aatacggtgc tattaatgct gaaaattgta atattattat tatttatgtc taccattaca
     1141 tgaaaaatac acgtaaatat gggaatcagg cgactgccta tgcttttatt gaatcaggag
     1201 aaatagccca gaatattcaa ttgactgcaa ctgccttaac ttatggaagt attgatattg
     1261 gtggttataa taaggaatat ctccaagaat tattagattt agatgggcta ggagagcatg
     1321 tgattcacat gacactcgta ggaactaagg agtctcaatg aaatatcaac ttaatagtaa
     1381 tgttcgtgtc gtgacatttc aagatacatt ttgttttaga aaaggaatct gggactttaa
     1441 tgaagccatt ctagatttga cacaagaacc tcaaaattta aagatagttt atcaagaaat
     1501 agtttcacag ttggttaagg gagtagccat tgatactgaa acttatgaaa atactttaga
     1561 acctgagacg ttcgctaatt taatggaagt catttcaggc ttgtattaca atgatatgct
     1621 gatgcttgag gatgactaca atcttgaaga aaatgtcatg aaaattttaa tgggtaattt
     1681 ccgttttatg gcgcaagcag gtcatatggt aagcaatgct cctgttttgt ttatcagtga
     1741 ttcgacttat gttaatgaat ctgctgagtt gctagctgaa caactgcatt tacaattgca
     1801 agtggctagt gatgacttaa aaatgttaat tcagcagaca gatgttagtt cgcgtttaga
     1861 tgctttggag catcgccgta acatgaattg tttatcagaa gccttgtgcg attaccaaag
     1921 tattattatt tgtcaagaac gcttgaatat tatgatgttg cgacacttga atgaagttag
     1981 cgtagctatg aaaaaacaat tagtgattgg ttttgtagat ggaccattct tgcacacttg
     2041 tactttgaat cctccccata gtgctgactt tgatagctta gaaagaagag ttttggcacg
     2101 tttacaagac tcaactctct atcaacattt tgctaatcag gttttaccag ccacacaaga
     2161 tgtgagccag gcctatttac cattgttaaa tgttttaatg aatttagtcg ttagcgaagc
     2221 tttcattatt gttcaaacag gtagttctaa atttgaggga cgtttattaa gcatttactt
     2281 accgacgctt gagattcagg tccaagatat tttgaaaatg tctaattcac agacacaagg
     2341 agctttggca aaattaaagt atgaggatca gcagatatca acacgtgaga ttgttaaaaa
     2401 actccttgac gagtaaccta taaggagaat ctattatgtt atactattat ccttctttta
     2461 atcatatttt tgatgaatta aaaagtttaa gtggcaatcg aacaggtatc ttaaatcaat
     2521 ctcaagttcc agtttgcaac catcctcacg atgtttactt aaaaagtatt actggccaaa
     2581 tgccagatta tcataaacaa tttataggtg agttgagtca agttagttat cacattattg
     2641 gttacggcag ttactacgaa gaagcactta tcaagtattt aggagaaagt attgaacgct
     2701 atgctactgt aattgccgga gacttactat cagatcggat tgtctatgct tcttataatg
     2761 agttaaaact gttacataaa gtgatgcctt tagaatactt gcaagtgttt acccaagaac
     2821 agatcgcatt atcttgtgac ttgcaaatga tgatgtgcga taaaatggta actgagaacg
     2881 atgtattggg ctgggttaaa tgtcccatgt tctttgagga cgcagagatg tatgttccag
     2941 ctcaaatgct ttgtgttggg tataaaacaa atgagactgt aggagaaaga cggataatac
     3001 caggattttc tacgggaaca gcttctcaca aaacgttaga agcggctatg tgcaacagtt
     3061 tgattgaata catccagatc gattcaatga tgcttagctg gtatactaag aagccttgcc
     3121 ccaagattat tgttgatgat cctgatattg aagtgatttt agaagaagct agactgggaa
     3181 aagacagtct atacgacatt attcctatcg atatgactgt aggagaagat aatcctcttt
     3241 atacgtttgg aatcattctc aaaaataaat atgatgaagg gccttacctc ttgtttggag
     3301 tgcaggctgg gttagatcct aaacatgctt tgctaagagg gatcatggaa gcttcagcta
     3361 ttagctatag ttactactat aatcttttat atcaaaaagc ttcccttgca aatattgaat
     3421 gcgaagaacc tttgttttta gatttagata gtaatgtctt ctattatgca catcccaagg
     3481 accaagacca taaatggaag gcctttgaac ctttgatttc aggagaagtt ctgttaagtg
     3541 atttggaaga tcattctggt aaagataaaa aagaagattt gaagacttta ttagcttatg
     3601 ctaagaaagt cagtcctaat gctgtctttt tagatattac acctcctgaa gctctagaaa
     3661 aaggctggta cgtcacgcga gttctgatgc cagaacttct tgaaatgtgt ataccagcat
     3721 ttccttttgc taatcatcca cgaatgagac agtttggagg tgtaacaaat gcctttgtcc
     3781 atccaatgcc ttaatctttg ttttttgttg gtaacttttt gtccttcaat tcctatgcaa
     3841 gctatttttg gaaaagaaga tagtggttat gcatttaatt taattggttt tctaagagca
     3901 actctgattt atgacatttt ggctttggta agtatttatg ttttgtcacc taaaataacc
     3961 ttgtctcttg aaagtattga tagtaagacg ttctttatgg gacttgtatt ttgcgtcttt
     4021 atagtactga ttgaactcgt tttcttacat ggtttacgtt gttggcaaaa aaaggaatgg
     4081 cttcctgcaa ctttctcgtt tgtaggaacg acaaatgatt ggtctaagat tggctatcct
     4141 ttattactag ctttatttga agagacgatt tatcgttttt tgtggtttaa tatattagct
     4201 tttcaatggc atttaccaac aattattgtt ttaattgtaa ctagtttttg ctatgcttta
     4261 aatcacttat tgatggggaa atccattttt tatgctaagt tggtaacagg catcatttat
     4321 ggtagtattt acatgctgac tagtcaattg tggttggttg tgataatgca tgtaggaggt
     4381 aacttgctag ttgagtgtct tagccatctt caaacaaaaa agaagaagga ggtgacatga
     4441 tgctattggt tttgctgtcg tttttaggtc taactactct ttggttgacc agttatcgaa
     4501 gacgttgttt ggctagatgg catcttcaat ggttaatggc cttatcctat caagattttt
     4561 tagatgtctt actgagtctg tttcaatttg tcgttattat acttgttttg ttcttttact
     4621 cagctactat taatctagga gaggttctga cctttttgac acaaactagc tggcattggc
     4681 agattctttg ttatttagtg ttgtatctta tggccatcat agagatgacg cttttagttt
     4741 taattcttat ctttgatgtt ttgctccaga aagatagtcg attgctcttt aaaaaaataa
     4801 cttggctgcc ttttagacaa gataaaccag tcttaagtat acttttgcta ggcatagtta
     4861 ttctagtaga cagtctcttt tatttgggct tgcttatatt attaggagaa cagtcgctaa
     4921 caagtttagc aacgcttatt ttaggctatg ggatagtcaa ggcttgtcgc tattcaggtt
     4981 ggttaaatgt actattagct ttttgtcttt tcactatagt tggactatgg gcagttacgg
     5041 caactttatt atatggttgg ctagtaggtg cgatgatatt aacggtgact tatttgatga
     5101 ttagttgcaa agagtattag caatattaaa aaggagtgat atatgagttt tgtacaatta
     5161 acaaatgttg tcaagtccta caaaaacggc aagaaagctg tcaatgatgt ttccttgtct
     5221 attgaagcag gtaatattta tggtttgtta ggaccaaatg gtgctggtaa gtctaccttg
     5281 attaatctta tcttaggctt gatccctttg agttccggta aaattactgt tttagggcaa
     5341 tcccaaaaga ctattcgaaa aataagttcg cagataggtt atgttcctca agacattgct
     5401 gtttatccag acctaactgc ttatgaaaat gtagaactat ttgggtcact ttatggctta
     5461 aagggagctc agcttaaaaa acaagttcta aaaagtttag aatttgtggg actacactcc
     5521 caagctaagc agtttccaag tcaattctca ggaggaatga agagacggtt aaatatcgct
     5581 tgtgcgctag ttcattcacc taaattaatc atttttgacg aaccgactgt agggattgat
     5641 cctcaatcac gtaatcatat tttagagtcg attcgtttgc taaacaaaga aggtgccaca
     5701 gttatttata cgacccatta tatggaagaa gtagaggctc tttgtgatta tatttttatt
     5761 atggatcatg gtcaagttat tgaagaagga cctaaatttg aactggaaaa acgttacgtt
     5821 gcaaatctag caaaccagat tattgtaact ctaacagatt cacgtcattt ggaactggca
     5881 gataagcctg attggtcttt gatagaagat ggagaaaaac tcatgttgaa gattgataat
     5941 agtgatatga catcagttgt tcatcagctc acacaggcca atattacttt tagcgagatt
     6001 agacataacc atttgaattt agaagaaatt ttcttacact taacaggtaa gaagttacga
     6061 gattagaggt tactatggtt ttatttcatt taatcaaaaa agaaagttta cagattttta
     6121 gaaaccgaac agccttattg atgatggtga tttttccaat tttgatgatc gttattttaa
     6181 gttttgcctt taaatcaagt tttaatactg cgacaacagt ccctaaattg accattcgtt
     6241 accaattaga gggtgaaaaa acggattacc agaaaaattt tcttgctttt ttaaaagttt
     6301 taaaccaaaa acttcattta gaggctaaac ctagtaattc tcttgaaaaa gatcgacaaa
     6361 gggtcagtga aggagcctta acggctgttt tagaagtgaa gaagaatcag accattaagg
     6421 ttattactaa taatattaat cagcaaaatg cagatttaat caatatgcta gtaaaaaatt
     6481 atgtagataa tgctaaaact tatgactcga tagcagctct ttatcctcaa caattaaatc
     6541 atatcagaaa gcgaagtgtg gactatgtta aggtcagttc aatacagaca agtaaaggaa
     6601 tgacatcagc tgattattat gctatttcca tgtttaccat gattactttt tatagtatga
     6661 tgtctgcgat gaaccttgtt ttgtcagatc gtcaacaacg gattacaaat cgtattcgct
     6721 taacaggagt ttctccaagt cttttagtct ttgggaaatt aataggtgct atgttagcaa
     6781 caactgttca attgagtctt ttatacattt ttacaaggtt tgttttacga gttaattggg
     6841 gcactaatga gtggatgctt attggtataa cagcctcctt agtctatctc tctgtagcta
     6901 taggtatcgg gctaggcata agcattaaaa atgaggcttt tttaacggtt gcatccaata
     6961 ctattattcc tatattcgcc tttttaggag gcagttacgt tccgttgaca acattacaca
     7021 gctctattat taatcaattg tcgaatatat ctcctattaa atgggttaat gatagtttgt
     7081 tctatcttat ttttggtggt caatataatc cgattcctgt aactttgatc gttaatatta
     7141 gtatcggaac aatttttata atattggcat taataggtat gagaaagcag gtgacgacat
     7201 gatttgtttt ataaaaactc tatttgttaa gataaagcgc aaaaaaacaa gttatgtgac
     7261 attcttttta atgcctattt taacgacact tttggcttta tctttgagtt tttcaaataa
     7321 taatcaggct aaaattggga tattagataa agataatagt cagatatcta aacaatttat
     7381 tgctcaacta aagcaaaaca aaaagtacga catttttacc aaaatcaaaa aagagcatat
     7441 tgatcactac cttcaagata aatctctaga agcggtattg actattgata aagggttctc
     7501 agataaagtg ttgcaaggaa aatctcaaaa actcaatata cgttcgattg ctaatagcga
     7561 aattacagag tgggtaaagg cacagaccaa ttatctattg gaaaactata atattatagg
     7621 ggatgtggct ctaggtaacg aagatacttt taatagaatt ctccaaaaaa accaacagtt
     7681 aaactatgat gttaagcaag taactttgac agaccgatca cgtagcaaag cagtatcttc
     7741 cacaacgaca ggatttttgc ttattctaat gttaggtagt acgagcgtga tttacagtgg
     7801 tattttggca gataaatcta gtcagttata ccatcgatta atgttatcga acttatctcg
     7861 ttttcgctat atgttgagtt acgtctgtgt aggatttgtt gcttttacga ttcaaattgt
     7921 cattatgctg agtttgttaa aagtatttaa tattagtttt tttgttccga catcacttct
     7981 tttgattatt tttttccttt ttagtttatt agcgataggc tttggcctgc tgattggagc
     8041 tatcacacaa aattcccaac aaagtagcca gttagctaat cttattgtaa tgccgacttc
     8101 aatgttggct ggctgtttat ggccattgtc tattacgcct tcttatatgc aagcaattgg
     8161 taagttatta ccacaaaatt gggttttatc agccatagct atttttcaaa gtggagggac
     8221 cttatctcag gcttggccat acttgttagc tctaatggga acagcactag ctttgattat
     8281 tttttcaagt ttattattaa aacctaccag actataatta gtcttttgtt aaaaaatact
     8341 ttcctaatta gctaactacc aggtagccct tttattggct tatatgcaat aaaagggttg
     8401 ctttttgctt atattttctc tagtgcactt atcttttgtt aaaagcgatt gcgatgttac
     8461 tgttgttact gtgtttttta gagagcatat tatcaaaagc gacctctcta gaaagttcg
//
LOCUS       DAUCLR010000079         8417 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.079, whole genome shotgun sequence.
ACCESSION   DAUCLR010000079 DAUCLR010000000
VERSION     DAUCLR010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8417
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.079"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..144
                     /locus_tag="VGT87_001491"
     CDS             <1..144
                     /locus_tag="VGT87_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009431731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HEQ3814328.1"
                     /translation="FKKAVKESERKADRKDSITKTINKFKDMAKDTISKDKVKNKHKE
                     QSL"
     gene            141..875
                     /locus_tag="VGT87_001492"
     CDS             141..875
                     /locus_tag="VGT87_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HEQ3814329.1"
                     /translation="MISNLKTFENKNFGKLTVIEKDGEFFFIANEVATMLGYVNPRKA
                     VYDHVDEEDKGVTKWNTPGGIQNISIINESGLYSLILSSKLPQAKIFKAWVTREVLPS
                     IRKNGGYIAGQEKKTNEELLADAILVANRIIAEREEEIEVLRPKADYYDKLVDYSLLT
                     NFRNTAKELGIPQNQFISFLMDKGLIYRDKKKKLLPYADKNKGYFEVKEWVDPLGTLV
                     GIQTFITPKGRHYLLILLDSEGFYDE"
     gene            868..2664
                     /locus_tag="VGT87_001493"
     CDS             868..2664
                     /locus_tag="VGT87_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006740602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEQ3814330.1"
                     /translation="MNKILEAILSDIKNLIKIDNPKKFILSNIPYLSFFYIGNIFSKH
                     INSYVGGDIIDRIMAGISDIGTLSYIPSLNPRDLLVGVSVAALVKLIVYIKGKNKKKY
                     RQGKEYGSARWGESKDIAPYIDPKFENNVLITNTERLTMNSRPKNPKYARNKNVLVIG
                     GSGSGKTRFYVKPNLMQMHSSYVVTDPKGTLVLECGKMLNENGYDIKILNTINFKKSM
                     KYNPFAYLRSEKDILKLVQTIIANTKGDGEKAGEDFWVKAEKLYYTALIGYIYYEAPE
                     EEKNFKTLLDMIDASEVREDDETYMNPIDRLFEALEKKDPSHFAVKQYKKYKLAAGKT
                     AKSILISCGARLAPFDIRELRELMSEDKLELDKIGDRKTALFVIISDTDDTFNFVVSI
                     MYSQLFNLLCDKADDVYGGRLPVHVRCLLDEFANIGLIPKFEKLIATIRSREISASII
                     LQAQSQLKAIYKDHADTIVGNCDSTLFLGGKEKTTVKELSETLGKETIDLYNTSETRS
                     NQKSFGLNYQKTGKELMSQDEITVMDGGKCIYQLRGVRPFLSDKFDITKHKNYKLLED
                     YDKKNLFDVEEYLTNRDKVKLKSNYKINRLNI"
     gene            2683..2958
                     /locus_tag="VGT87_001494"
     CDS             2683..2958
                     /locus_tag="VGT87_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814331.1"
                     /translation="MCENRKSYLIILNINGEQFILESDTELTRDKKNYIEAICETMYD
                     ESNEWYEDIYDMSPYDIAELFEKTVKEEVGITVTFKAIDLEVSILEN"
     gene            3148..3459
                     /locus_tag="VGT87_001495"
     CDS             3148..3459
                     /locus_tag="VGT87_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006740599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEQ3814332.1"
                     /translation="MDREMININANLVKEAEISEFEKDGESVQVANFALVKKYGKGKE
                     YTNCSVYGEKVEIVKEFEKGDLIHVFGYFKENKKGDKVYKNFIVKSLNKIENKKENEE
                     E"
     gene            3461..3676
                     /locus_tag="VGT87_001496"
     CDS             3461..3676
                     /locus_tag="VGT87_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HEQ3814333.1"
                     /translation="MEFFTAGVGVLKTLVTAIGAGLGAWGVINLMEGYGNDNPGAKSQ
                     GIKQLMAGGGIVLIGIKLIPLLANVLN"
     gene            3687..4550
                     /locus_tag="VGT87_001497"
     CDS             3687..4550
                     /locus_tag="VGT87_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814334.1"
                     /translation="MFGIFDKLTEFFKDMLLGGIKANLESMFLDINDKVGVIATDVGK
                     TPMGWNGEVYNFIKNINDNVIVPIAGLIITAVLCIELINMVMQKNNMHDTDTFEFFKY
                     IIKMFIAVYLASHAFEFSMAVFDVAQNLVNKAAGVITTSATVSGDQIVAMVDTLKEKE
                     IGELLMILVETSLVRIAIQCISLTITLIVYGRMFEIYVYSSVSSIPFATMGNKEWGQI
                     GTNYIKGLFALGLQGLFLMVCLGIYTVLIRTVQITDIHASLFSILGYALLLGLMMFKS
                     GTVAKSIMNTH"
     gene            4560..4850
                     /locus_tag="VGT87_001498"
     CDS             4560..4850
                     /locus_tag="VGT87_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814335.1"
                     /translation="MLKKKNCALLGLGILLGGTYEILKEKKRNEEIRNLKRRINRLGR
                     CHNEFVMYQGEYNDRNEEKQEELEERISYLEEETTSNYDHILELSKEEVEGD"
     gene            4852..5262
                     /locus_tag="VGT87_001499"
     CDS             4852..5262
                     /locus_tag="VGT87_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012797153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HEQ3814336.1"
                     /translation="MAYVPIPKDLDKIKTKVAFNLTKRQLIGFSVAGLIGIPTYLFMK
                     KYLPNDVSIIVMLIVTLPIFFITLYEKDTLTFEKYFKFFYLHKFYQPTKRIRKEAYIE
                     AKKKANQRLKSKGKTIKKRPKGSKKAKNKERSNK"
     gene            5153..7576
                     /locus_tag="VGT87_001500"
     CDS             5153..7576
                     /locus_tag="VGT87_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002836672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEQ3814337.1"
                     /translation="MKQRKKQISDLNLREKQLKKDRKEVRRLKTKKDPTNSLLSVLLK
                     KEKKRFTVEDTIPYIRMLPEGICQLDEKNYSRTISFQDINYQLALEEDRDLIFNQFAN
                     FLNSFDPSVHIELSYVNQLGRNKDLQDAIKIADKGDFYDDVRKEFREMLKLQLAKGNN
                     GLKKMKYITFTTEADNLEQARAKLNRLEVDILSNFKSMGVRAESLDGEERLRLVHDML
                     NPDKNFDFSYKDLKKKESTKSHITPNIFNFAPANNFKFGKYIGAVSHFQILASELSDR
                     MLSEFLDIDDNIYVAFHIDVVEQAEAIKLIKRKNTDLDRMKIEEQKKAVRAGYDMDII
                     PSDINTFGADVKSMLSDLQNRDERLFVVTIVMMNFARTNQKLENTIAQISSIANRHNC
                     QVKRLSHQQEQGLVSVLPLGLNQVEIKRFLTSSSTAVFMPFTTEELFIDSANSLYYGL
                     NALSQNLIMADRKKLKNPNGLILGTPGSGKSFSAKREMANAILVTDDDVIICDPEGEY
                     SNLVKQFNGEVIKVSAKSKDYLNPLDINMNYGDGDAPLKDKANFIMSMLELVVGGSGL
                     TAAEKSVIDRCLPKIYQKYFEDPKPENMPILGDLYDMLLSQEEGVGRKLATEMEIYVK
                     GSLNVFNNRSNVDLNRQLLCFDIKELGTQLKKIGMLVIQDQVWNKVSLNRGSKSTRYY
                     IDEFHLLLKDPQTASYSVEIWKRFRKWGGIPTGITQNVKDLLTSQEIENIFDNTDFVL
                     MLNQASGDRDILAKKLKISPYQLNYITNSNAGEGLLFFGNTIVPFIDKFPKDTMLYKL
                     MTTKPEEAK"
     gene            7584..>8417
                     /locus_tag="VGT87_001501"
     CDS             7584..>8417
                     /locus_tag="VGT87_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002839222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814338.1"
                     /translation="MDKKLKKDFQKKIIRNRDTPEKNMDSKLVHSDDYTNKIIKTKDR
                     FGDKISEKESKLIHENVLAKDQKQDKLKEFQKSKNKEKIRKEVLDNKYKTEEIKQANL
                     EIRTDESFKLDEELDVDFKKVNFDSENSRNINSNKKTTDDISAKAQPINNKKASSKRQ
                     VLKNYEDKLIHSKDKFQDKINERESKRIQTSEDKPIEAKKSKRIYRKDKLVKDEVSKN
                     ESNTNIDKMQKQKLYQEKKFIDKEKISKEIDKENKPSEVDTDKTFDNSELKDNNQEFI
                     KD"
BASE COUNT         3409 a         1070 c         1545 g         2393 t
ORIGIN      
        1 tttaaaaaag cagttaagga atctgaaaga aaggccgata gaaaagattc aataacgaag
       61 actataaata agtttaaaga tatggctaag gataccatta gcaaagataa agttaaaaat
      121 aaacataagg agcaaagctt atgataagta atttaaaaac atttgaaaat aagaattttg
      181 ggaaactcac tgttatagaa aaagacggtg agtttttctt tatagctaat gaagtagcaa
      241 ctatgttggg atatgtcaat ccaagaaaag ctgtttatga ccatgtagat gaagaagata
      301 agggtgtaac gaaatggaac acccctggag gaatacagaa tatttcaata attaacgaat
      361 caggattgta ttcacttatc ctttcatcaa aactaccaca agcaaaaata ttcaaagctt
      421 gggtaactag agaagtctta ccaagtatta gaaagaatgg aggatatata gctgggcaag
      481 aaaagaaaac taatgaagag ttacttgcag atgcaattct tgtagctaat agaattattg
      541 ctgaaagaga agaagaaatt gaagtattaa gaccaaaggc agactattat gacaaattag
      601 tagattatag cctacttaca aactttagaa atactgccaa agagttagga ataccacaaa
      661 atcagtttat aagtttttta atggataagg gattaattta tagagataag aaaaagaaac
      721 tcttacctta tgcagataag aataagggat actttgaagt aaaagaatgg gttgatccac
      781 taggtacact tgtaggcata caaacattta taacaccaaa gggaagacac tacctactaa
      841 ttttattaga tagtgaaggt ttctacgatg aataagatat tagaagccat tctttctgat
      901 attaaaaact taattaaaat agacaaccca aagaaattta tattatcaaa cattccttat
      961 ctatcctttt tctacattgg aaatatcttt tctaagcaca tcaattctta tgttggagga
     1021 gatattattg atagaataat ggcgggaatt tctgatatag gaactttatc ttatatacca
     1081 agccttaatc caagggactt gttagtaggt gtttcagttg ctgctcttgt taagctaatt
     1141 gtttatatta aaggtaaaaa taaaaagaaa tacaggcaag gtaaggaata tggatctgca
     1201 agatggggag aaagcaagga tattgctcca tacattgacc ctaagtttga aaacaatgta
     1261 ctaataacta atactgaaag actaacaatg aattcaagac ctaaaaaccc taaatacgct
     1321 agaaataaaa atgtgttagt gattggtggt tctggatcag gtaagacaag attttatgta
     1381 aagccaaatc taatgcaaat gcactcttcc tatgtagtaa cagaccctaa aggcacactt
     1441 gtgttagagt gtggaaaaat gcttaatgaa aatggatatg acataaagat tttaaacaca
     1501 ataaacttta aaaaatctat gaaatacaat ccctttgcct atcttcgatc agaaaaagat
     1561 attttaaaac ttgtccaaac aattattgct aacaccaagg gagatggaga aaaggcagga
     1621 gaagatttct gggtaaaggc tgagaagcta tattacactg cccttatcgg ttatatctat
     1681 tatgaagcac cagaagaaga aaagaacttt aagacacttt tagatatgat tgacgcaagt
     1741 gaagtcagag aagatgacga aacctatatg aacccaattg ataggctctt tgaagctctt
     1801 gaaaagaaag acccaagtca ttttgcagtt aagcaatata agaaatataa gcttgcagca
     1861 ggaaaaactg ctaagtcaat tctaatatct tgtggagcaa gacttgcacc ttttgatata
     1921 agagagctta gagaactaat gagtgaagac aaactagagc ttgataaaat tggagatagg
     1981 aaaactgcct tgtttgtaat aatatcagat acagatgata cctttaactt tgtagtatca
     2041 ataatgtatt cccaattatt taatttacta tgtgataagg cagatgatgt gtatggtgga
     2101 agacttccag ttcatgttag gtgtctactt gatgagtttg caaatattgg cttaattcct
     2161 aaatttgaaa aattaatagc aacaattcgt tcaagagaaa tatcagcaag cataatacta
     2221 caagcacaat ctcaattaaa agcaatatat aaagaccatg cagatacaat agttggtaac
     2281 tgcgattcaa cactttttct aggaggaaaa gagaaaacaa cagtaaaaga attatctgaa
     2341 accttaggaa aagaaaccat agacctatat aacacatcag aaacaagatc caatcaaaaa
     2401 tcttttggtc taaattacca aaaaactggc aaggaactta tgagtcaaga tgaaataact
     2461 gtaatggatg gcggtaagtg tatataccaa ctaagaggag taagaccttt cttgtctgat
     2521 aaattcgata ttacaaaaca taagaattac aagttattgg aagattatga taagaaaaac
     2581 ttgtttgatg tagaagagta tttaacaaat agagataagg taaagttaaa atcaaattat
     2641 aaaataaata ggctaaatat ttgagtttat ggagaaagca aaatgtgtga aaatagaaaa
     2701 tcatatttaa ttatattaaa tataaatggt gagcagttta ttctagaaag tgacacagaa
     2761 ctcacaaggg ataagaaaaa ttatattgaa gcaatatgtg agacaatgta cgatgaaagt
     2821 aatgaatggt atgaagatat atatgatatg tccccatatg atatagctga gctatttgaa
     2881 aaaacagtaa aagaagaagt aggaataact gttacattta aagcgataga ccttgaagta
     2941 tccattttag aaaactagaa ataaagtgtt gataggcgat agaaattaaa aaatctatcg
     3001 tcttttttta tactcaaaat taggaggttt aaatggtaag gataagaagt cccacttaaa
     3061 taatttaata tataagaaat ttagcgattg aaatataagt tcagtcgctt ttttaattga
     3121 aatttttaga ttaaggagaa gaaattaatg gatagagaaa tgataaatat taatgcaaat
     3181 ttagttaagg aagctgaaat ttcagaattt gaaaaagatg gagaaagtgt tcaagtagca
     3241 aattttgctc ttgtaaaaaa atatggaaag ggcaaagaat atacaaattg ctcagtatat
     3301 ggagaaaagg tagaaattgt aaaagaattt gaaaaaggag atctaatcca tgtctttgga
     3361 tatttcaaag aaaacaaaaa aggagataag gtctacaaaa actttatagt taaatcacta
     3421 aataaaatag aaaataagaa agaaaacgag gaggaataat atggaatttt ttacagctgg
     3481 agttggagtt ttaaagacac ttgtaactgc aattggtgca ggtttaggtg catggggagt
     3541 tattaactta atggaaggtt atggtaatga taaccctggt gctaaatctc aaggtattaa
     3601 gcagcttatg gctggtggag gaattgtact aatcggtatt aaattaattc cactacttgc
     3661 aaatgtttta aattaggaga aagtctatgt ttggtatctt tgacaagcta actgaatttt
     3721 ttaaggatat gctactcgga ggtatcaaag caaatcttga gtccatgttc ttagatataa
     3781 atgacaaggt aggagttatt gcaactgatg ttgggaagac accaatgggt tggaatggag
     3841 aagtatacaa cttcataaaa aatattaatg ataatgtgat tgttccaata gcaggtctta
     3901 tcataacagc agttttatgt attgaactca taaatatggt tatgcaaaag aataatatgc
     3961 acgatacaga tacttttgag tttttcaaat acattataaa gatgtttata gcagtctacc
     4021 ttgcaagcca tgcctttgaa ttttcaatgg cagtctttga tgtggcacaa aatcttgtaa
     4081 acaaagcggc aggggtaatc actacttctg ccactgtttc aggagatcag atagttgcaa
     4141 tggttgatac attaaaagaa aaagaaatag gtgagctttt aatgatatta gttgaaacaa
     4201 gccttgtaag gattgcaatt caatgtatat ctttaactat taccttaata gtatatgggc
     4261 gtatgtttga aatatatgtc tactcatcag tatcatccat accatttgcg actatgggaa
     4321 ataaagaatg gggtcagatt ggaacaaatt atatcaaggg actttttgcc ttgggactac
     4381 aaggtttgtt tttgatggta tgtttaggta tctacaccgt tttaataaga acggtacaga
     4441 ttacagatat tcacgcaagc ttgtttagta tattaggata tgctctacta cttggactta
     4501 tgatgtttaa gagtggaaca gttgcaaaaa gtattatgaa tacgcactag gaggaataaa
     4561 tgttaaaaaa gaaaaattgt gctttattgg gacttggaat tttattagga ggaacttatg
     4621 aaatcttaaa agagaaaaaa agaaatgaag agattagaaa cttaaagagg agaataaata
     4681 gactcggaag atgtcacaat gaatttgtta tgtatcaagg cgaatacaat gacagaaatg
     4741 aagaaaaaca agaagaatta gaagaaagaa ttagctacct ggaagaggaa acaacatcta
     4801 attatgacca tatacttgaa ctttccaaag aagaagtaga gggagattaa aatggcatat
     4861 gtaccaatac caaaagattt ggacaagatt aaaacaaagg ttgcctttaa cttaaccaaa
     4921 agacaactta taggtttttc tgtggcagga ctaattggta taccaaccta tttatttatg
     4981 aagaaatatc tacctaatga tgtttctatc attgtaatgc taatagtaac cctgccaatc
     5041 ttttttataa ccttatatga aaaagatacc ttaacttttg aaaaatattt taaatttttc
     5101 tatcttcata agttttatca accaactaag agaataagaa aggaggcata cattgaagca
     5161 aagaaaaaag caaatcagcg acttaaatct aagggaaaaa caattaaaaa aagaccgaaa
     5221 ggaagtaaga aggctaaaaa caaagaaaga tccaacaaat agtcttctaa gtgtactttt
     5281 aaagaaagag aaaaagagat ttacagttga ggatactatt ccctatataa gaatgttacc
     5341 agagggcatt tgccagttag atgaaaaaaa ttattcaagg acaatttcat ttcaagatat
     5401 aaattaccag ttggcattgg aagaagatag agatttaatc tttaaccaat ttgccaactt
     5461 tttaaattct tttgatccaa gtgtccacat tgaactttcg tatgtaaatc aattaggaag
     5521 aaataaagac ctacaagatg caattaaaat tgctgataag ggagacttct atgacgatgt
     5581 aagaaaagag tttagggaga tgttaaagct tcaacttgca aagggcaata acggacttaa
     5641 aaagatgaag tatataacct ttacaacaga agccgataac ctagagcaag caagagcaaa
     5701 gttaaataga cttgaagtag atattttatc taactttaaa tctatgggag taagagcaga
     5761 aagccttgat ggtgaagaaa ggctaagact tgttcacgat atgttaaatc cagacaaaaa
     5821 ctttgatttt tcatataaag atttgaagaa gaaagagtct acaaagtcac atataactcc
     5881 caatatcttt aactttgcac cagcaaataa ttttaaattt ggtaaatata ttggagcagt
     5941 aagccatttt caaatactag caagcgagtt atcagacaga atgttatctg agtttttaga
     6001 tattgatgat aatatttatg tagcttttca tatagatgta gttgaacaag cagaagccat
     6061 taaactcatt aagagaaaaa acacagacct ggatagaatg aaaattgaag aacaaaagaa
     6121 agcagttcgt gctggatatg atatggatat tattccttca gatataaata cttttggagc
     6181 tgatgttaag tccatgttat ctgacctaca aaatagagat gaaaggctct ttgtagttac
     6241 cattgtaatg atgaattttg ctagaactaa tcaaaaatta gaaaatacaa ttgctcaaat
     6301 ttcatcaatt gctaacagac ataattgtca ggtaaaaaga ttatctcatc aacaagagca
     6361 aggactcgtt tctgtcttac ctctaggact taatcaagtc gagataaaaa gatttttaac
     6421 ttcatcatca acggcagtat ttatgccttt tacaacagaa gagctattta ttgattcggc
     6481 aaattctttg tactatggat taaatgccct tagccaaaac ttgattatgg cagataggaa
     6541 gaaactcaag aaccctaacg gactaatatt aggaacacca ggttctggta aatctttctc
     6601 ggcaaagaga gagatggcaa atgcaattct tgtcacagat gatgatgtaa ttatttgtga
     6661 tcctgaagga gagtattcaa accttgtaaa acaatttaat ggagaagtta ttaaagtttc
     6721 agcaaagtca aaggactacc ttaatccatt agatattaat atgaactatg gagatgggga
     6781 cgcaccttta aaagataagg caaacttcat aatgtctatg cttgaacttg tagttggagg
     6841 atctggtctt actgctgcag aaaaatctgt tatagatagg tgcttgccaa agatatacca
     6901 aaagtatttt gaagacccta agccagaaaa tatgccaata ttaggagatt tatacgatat
     6961 gctcctatct caagaagagg gagttggtag aaaactagca acagaaatgg aaatttatgt
     7021 taagggaagt cttaatgtct ttaataatag gtcaaatgtt gatctaaata ggcaattgct
     7081 ttgctttgat ataaaagagc taggaacaca acttaaaaaa ataggtatgc ttgtaattca
     7141 agaccaggtt tggaacaagg tttccctaaa tagaggaagc aagtctacaa gatactatat
     7201 agatgagttc caccttttat taaaagaccc acaaactgct tcatattcag tagaaatctg
     7261 gaaaagattt agaaaatggg gaggtattcc aacaggtata actcaaaacg taaaagacct
     7321 tttaacaagt caggaaattg aaaatatctt tgataataca gactttgttc taatgttaaa
     7381 tcaagcatct ggagatagag atattcttgc taagaaatta aaaatatcgc cttatcagtt
     7441 aaactatatt actaactcaa atgcaggtga gggactatta ttcttcggaa atactattgt
     7501 tccatttata gataaattcc ctaaagacac catgctatat aagctgatga caacaaaacc
     7561 agaagaagct aagtaggtgt gatatggata aaaaactaaa aaaagatttt caaaagaaaa
     7621 ttatacgaaa tagggacact cctgaaaaga atatggatag taaacttgtt cattcagatg
     7681 attataccaa taagattatt aaaactaagg ataggtttgg agataaaatt tcagaaaaag
     7741 aatctaaact tattcatgag aatgtattag ctaaagatca aaaacaagat aaactaaaag
     7801 aatttcagaa gtctaaaaac aaggaaaaaa tcagaaaaga agttttagat aataaatata
     7861 agactgaaga aataaaacaa gctaatcttg aaataaggac agatgaaagc tttaaacttg
     7921 atgaagagtt agatgtagac ttcaaaaaag taaattttga tagtgaaaac agtagaaata
     7981 ttaattctaa taaaaaaaca acagatgata tttcagctaa ggctcaacca ataaacaata
     8041 agaaggcatc aagtaaaaga caagttctta aaaattatga ggataaactt atccatagta
     8101 aggataaatt ccaagacaaa attaacgaga gagagtctaa gcgaatccag acaagtgaag
     8161 ataaacctat tgaagcgaag aaaagtaaga gaatatatag aaaagataag cttgttaaag
     8221 atgaagtaag taagaatgaa agtaatacta atatcgataa aatgcaaaag cagaaacttt
     8281 atcaagaaaa gaagtttata gataaggaaa aaatttctaa agagatagat aaagaaaata
     8341 agccaagcga agttgataca gataagactt ttgacaattc tgagcttaaa gacaataatc
     8401 aagaatttat aaaagat
//
LOCUS       DAUCLR010000080         8400 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.080, whole genome shotgun sequence.
ACCESSION   DAUCLR010000080 DAUCLR010000000
VERSION     DAUCLR010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8400
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.080"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..349)
                     /locus_tag="VGT87_001502"
     CDS             complement(<1..349)
                     /locus_tag="VGT87_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEQ3814339.1"
                     /translation="MTDKALSKRSLQNLTQFNKETRRIARESMEIALLNLLETKPLGD
                     ITISELVTKAGVSRNAFYRNYTSKEAIIEQLLVGVIRRIFRGLKQFDIKTQAYQAWLY
                     LFTEAKKEAQLLKM"
     gene            642..1484
                     /locus_tag="VGT87_001503"
     CDS             642..1484
                     /locus_tag="VGT87_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEQ3814340.1"
                     /translation="MTWKIVTDSGCDLRSLTRQSKELRFERVPLTLQIGTEIFRDDDG
                     LDIDNMMTTMYQSSKATTSSCPSPEAFLQAYRGADNVIVMTITGTLSGSHNSARLAKN
                     ELLEENPNVNIHLIDSLSAGGEMDLLVLELERLINLGLSFEEVVKQITAYQQKTRLIF
                     VLAKVDNLVKNGRLSKLVGKVIGLLNIRMVGKASNKGTLELLQKARGQKKAVSALIEE
                     IQKEGYAGGKVYIAHAQNPKICEQISEKIKSLYPDAVIQTGRTSGLCSFYAEDGGLLM
                     GYEI"
     gene            complement(1613..2335)
                     /locus_tag="VGT87_001504"
     CDS             complement(1613..2335)
                     /locus_tag="VGT87_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814341.1"
                     /translation="MATMKQYHKGDSMTKSQKEALYWMLSVLTITLIGGSCLIFGSHP
                     QTQDKVAKHSKSAASLLKKAVKAVNDADRLATAAAIQEAQKAVDKLAESSKKKTLQEQ
                     LNVAKAKQEQEDAATQAVKAAEETLNQNLKDIAQKAVNDLSNKGKKAALQSRLDAILL
                     AKPIIDEFPRQSGEITDNSYWTPFPGDVSDTYDNSQSPTLDPSSESSASDVTPQPSHP
                     DPIPPQTSSEPSDSGDKQSSKE"
     gene            2537..4168
                     /locus_tag="VGT87_001505"
     CDS             2537..4168
                     /locus_tag="VGT87_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEQ3814342.1"
                     /translation="MSVNWQDIAFHFFGGLGLFLFSIKYMGDGLQQAAGDKLRYYIDK
                     YTSNPFFGILVGIAMSALIQSSSGVTVITVGLVSAGLLNLRQAIGIVMGANIGTTITS
                     FLIGFKLGDYALPMIFIGAACLFFTSNKKLNNFGRIIFGVGGIFFSLNLMGDAMDPLK
                     SVSAFQNYLATLGDKPFQGVFIGTALTMLIQSSAAIIGILQGLFSGGLLTLQGAIPIL
                     LGSNIGTCITAVLAAIGSNIAAKRVAAAHVLFNLIGTIIFMIILVPFTSLMLWLQSKL
                     SLTPEMTIAFSHGSFNITNTILLIPFISLLAMIVTRLIPGEDEVVKYEALYLDRLLIT
                     QAPSIALGNAHKELVHLASYAIQAFEASYSYIMTADDKFGEKVKRYERAVDTIDEELT
                     TYLVDISNEALSPSENEVLAGILDSSRDLERIGDHSESLGILIEGIISKQIGFSISAR
                     QELTEMYQLTHCLTLDAIRAIVDSDTDLAQTIVTRHKEIEEKERRLRKTHIKRLNCGE
                     CTAQAGINFIDIISHYTRITDHALNLAEKVLSHQL"
     gene            4286..5434
                     /gene="nagA"
                     /locus_tag="VGT87_001506"
     CDS             4286..5434
                     /gene="nagA"
                     /locus_tag="VGT87_001506"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEQ3814343.1"
                     /translation="MTCYLKADCFYYPTEVKPAGYLSLHDGVFGEWTEIVPADAQIID
                     YTGYQIAPGLVDTHIHGYAGADVMDNSAQGIHQMSEGLLATGVTSFLPTTLTSTFEQL
                     EKVSGTIASVADQVKGAKIQGIYFEGPYFTEEYKGAQNPSYMKNPRLEEFDAWQKAAK
                     GLIKKIALAPERDGVKEFVSAVTKQGVTVALGHSNGTYQEAKEAVQAGASVWVHAYNG
                     MRGLIHREPGMVGAVYNLPNTYAELICDGHHVSPIACDILMQQKGHDHVAMITDCMRA
                     GGSPDGDYLLGEFSVVVANGTARLKESGNLAGSILKLKDGLRNVVAWGIATPAEAIHM
                     ATYVPAVSVGIDDVCGQIKAGHAADFIVLDKDLTLVATYLNGQKAFDA"
     gene            5556..6395
                     /locus_tag="VGT87_001507"
     CDS             5556..6395
                     /locus_tag="VGT87_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEQ3814344.1"
                     /translation="MVTTVKMTSGYEIPVLGFGTYQAADGEEAYQSTLAAIKAGYRHI
                     DTAAIYKNEESVGRAIKDSGVLREDLFITTKLWNDAHSYEGAKDALAASLGRLGLAYV
                     DLYLIHWPNPKALRNTWKEANAQAWQYMEEAVEAGLIKSIGVSNFMVYHLEALQETAK
                     ITPAINQIRLAPGCYQKEVVDYCKANEILLEAWSPLGQGEIFDNETMQQLANKYDKTV
                     AQVALAWSLAEGFIPLPKSVHDERIKENMAIFDVSLTPEDKKTIRYLSGMSAIPNPDT
                     TSF"
     gene            6527..7060
                     /locus_tag="VGT87_001508"
     CDS             6527..7060
                     /locus_tag="VGT87_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEQ3814345.1"
                     /translation="MTIFLVMLAMFTIRLVFLKKSITNEKAILAQGGQEFGAQNTKFL
                     TLLHIMIYVFAVIEALLKQIKFDGISFLGLLLMLLSVAVLYEVTRILGDIWTVKLMLA
                     KDHKYVDHWLFKTIKHPNYFLNIAPELVGIALLCHAKITAMLLFPCYIVVIYLRIREE
                     NKLLAEVIIPNGTRTKP"
     gene            7431..8348
                     /gene="glyQ"
                     /locus_tag="VGT87_001509"
     CDS             7431..8348
                     /gene="glyQ"
                     /locus_tag="VGT87_001509"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEQ3814346.1"
                     /translation="MSKKLTFQEIILTLQQYWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLASLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATSPVTAE
                     VTYGLERLASYIQEVDSVYDIEWAPGVKYGEIFLQPEYEHSKYSFEMSDQDMLLENFE
                     KFEKEASRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKKLGFPLLDEATRAILLAEDGE"
BASE COUNT         2529 a         1469 c         1800 g         2602 t
ORIGIN      
        1 tcattttaag aagctgagct tcttttttag cttcagtaaa aagatagagc caggcttgat
       61 aagcctgagt tttaatatca aattgtttta gtccacgaaa aatacgacgg ataaccccta
      121 ctaacagttg ttctataatg gcttctttag aggtataatt acgataaaaa gcgttacgag
      181 atactcctgc tttcgtcacc aattctgaaa tcgtaatatc acctaaaggt tttgtttcta
      241 gaagatttag taaggcgatt tccatagatt cacgtgctat gcgtcgtgtt tctttattaa
      301 attgtgttaa gttttgaagt gatctttttg agagtgcttt atcagtcatc acaaatacct
      361 ttcaagtgtt acatctgatg agttatcagt agcaaaaacc gacttaataa tgatataatt
      421 ttatgggaaa agtcttagtt tgtcaactaa agttagttat caggagcctc attacctcct
      481 tgtatagctc tattggatga ctttactaac tattgaaaag gctagtaata aaacatagga
      541 tttaatgatt agttattacc attagcctag acatcttagc gaaggcttaa taatctggtc
      601 tttgttaccg aacacaaatt agagaaaaag aaggataaaa aatgacttgg aaaattgtaa
      661 cagattcagg atgtgatctt agaagtttga ctagacaatc caaagagttg cgctttgaac
      721 gtgtgccttt gaccctacag attggcacag aaatttttag agatgatgat ggattagata
      781 tcgataatat gatgacaact atgtaccagt cgtctaaagc aacgacatct agctgtccaa
      841 gcccagaagc ctttttgcag gcctatagag gtgcagataa tgttatcgtt atgacaatta
      901 cagggacctt atctggcagt cataatagtg cacgactggc taaaaatgaa cttcttgaag
      961 aaaatcctaa tgtcaatatt cacctaatcg attctttatc agcaggtggc gagatggatt
     1021 tgctcgtact ggagcttgag cggttgatca atcttggatt atcctttgaa gaagttgtca
     1081 aacagatcac agcttatcag caaaaaacac gtttgatttt tgtgcttgcc aaggttgata
     1141 atttggttaa aaatggtcga ctgagtaaat tagtaggtaa agtaattggc ttactcaata
     1201 ttagaatggt tgggaaagct agcaataaag gaacgcttga acttcttcaa aaagcgcgtg
     1261 gtcaaaaaaa agcagtttct gctttgatag aagaaattca aaaagagggt tatgcaggcg
     1321 gaaaagttta cattgctcat gctcaaaatc caaaaatttg tgagcagatc agtgaaaaaa
     1381 tcaaatcttt atatcctgat gctgtgattc aaactggaag gacctcaggt ttgtgtagct
     1441 tttatgctga agacggagga cttttaatgg gctacgaaat ttaatgctat ctaacaaaac
     1501 aaaaagctag actcttttga tttgagctag cttttttaag atgtcaggaa gactaatcac
     1561 taagatagtc gtctaaaacg tttattgatt gattttacga aaaacttttg tattactctt
     1621 ttgatgattg cttgtctcct gagtcggaag gttctgaaga ggtctgaggt ggaattggat
     1681 caggatgact aggttgtggc gtaacatctg aagctgaaga ttctgagctt gggtctaaag
     1741 taggtgactg ggaattatca taagtatctg agacgtctcc tgggaaaggt gtccagtaag
     1801 agttatctgt tatttctcca ctttgtcttg gaaattcatc aataatgggt ttcgctagta
     1861 aaatagcgtc taaccgagat tgtaaagcgg cttttttacc tttgttactt aagtcgttaa
     1921 ctgctttttg ggcgatatct ttaagatttt gattgagagt ctcctcagca gctttaactg
     1981 cttgagtagc tgcatcttct tgctcttgct ttgctttggc aacgtttagc tgttcttgta
     2041 gcgttttttt cttagaagat tctgctaatt tatcaacagc tttctgggct tcttggatag
     2101 cagctgcagt ggctaaacga tcagcgtcat taactgcttt aactgctttt tttagtaaac
     2161 tagcagcact tttagaatgt ttagctacct tatcttgcgt ttgagggtgg gatccaaaaa
     2221 taaggcagga gccacctatt agagtgatag ttagaacact caacatccaa taaagcgcct
     2281 ctttttgtga tttagtcatg gaatctcctt tatgatattg tttcatcgta gccatattat
     2341 acctctaatt aaacgaaatg aaaagatttg tgcaaaaaca aatgatgtaa aatatcggaa
     2401 aagcctaaca ccttatcaaa ccaataatat gcaaatgttg acatcggtat acacagaaac
     2461 gcttaaaaaa accttgtgat aaagaccgta atacattaaa atagaataga ctaaaaaatt
     2521 gagcgaggac aatattgtgt cagtaaattg gcaagacatt gcatttcatt tctttggagg
     2581 cttaggtctc tttcttttca gtatcaagta tatgggagat ggtctgcaac aagcagcagg
     2641 agataaacta aggtattaca ttgataaata caccagtaat cccttttttg gtatcttagt
     2701 cggaattgcc atgtctgcct taattcagtc cagttcaggc gtcactgtga ttacggttgg
     2761 tttggtttct gcgggtttgc taaatttgcg acaagccatt ggaattgtta tgggggctaa
     2821 tattggaacg actatcactt cgtttttaat tggttttaaa ctaggagatt acgctttgcc
     2881 catgattttt attggtgcag cctgtctttt ctttacctct aataaaaagc tcaacaattt
     2941 tgggcgtatc atttttggtg tcggaggaat tttcttctcg ctgaacctaa tgggagatgc
     3001 catggatcct ttgaaatctg tgtcagcctt tcaaaattat ttagcgacat taggtgataa
     3061 acccttccaa ggtgtcttta tcggaacggc tttgaccatg ttgattcagt catctgcagc
     3121 cattatcggg attttacagg gtctgttttc aggcggtctt ttgaccttac agggggctat
     3181 tcctattttg ttaggctcaa atataggaac ctgcattaca gctgtcttag cagctatagg
     3241 ttcaaatatt gctgctaaac gtgtggcagc agctcatgtg ttatttaatt tgatcggtac
     3301 aattattttt atgattatcc tagtcccatt cacatcattg atgttatggc tacaatcaaa
     3361 gttgagttta acacctgaga tgactatcgc tttttctcac ggctctttta atatcactaa
     3421 taccatctta ttaattcctt tcattagtct actagctatg attgtgacta gactcattcc
     3481 aggagaagat gaagtggtca aatacgaagc cctttattta gatcgcttac ttattaccca
     3541 agccccttct atcgcactag gaaatgccca caaggaattg gttcatttag cctcttatgc
     3601 gattcaagct tttgaagctt cttacagtta tattatgaca gctgacgata aatttgggga
     3661 aaaggtcaag cgttatgaaa gagcagttga taccatcgat gaggagttaa ccacttattt
     3721 ggttgatatt tctaacgaag ccctcagccc aagtgaaaat gaagtcttgg ctggtattct
     3781 tgactcttca cgtgatttgg aaagaattgg agatcattct gagtctttag gcattctaat
     3841 tgaaggcatc atatctaaac aaattggttt ttcaatatct gctcgtcaag aattgacaga
     3901 gatgtatcaa ttgacgcatt gtttaacact ggatgccatt agagctatcg ttgatagtga
     3961 tacagatttg gcgcaaacta ttgtgactcg ccataaagaa attgaagaaa aagagcgacg
     4021 attacgtaaa acccacatta agcgcttgaa ttgtggagaa tgtacggcac aagcaggtat
     4081 taattttatt gatattattt cccactatac ccgcatcaca gatcatgcct tgaatttagc
     4141 agagaaagta ctttcacacc agctttaaaa aaagagaaaa cgtgaatgat catccacgtt
     4201 ttcttttttg aatccaaaaa gtggtatata ccatttacaa agtaaagaat aaggcgtata
     4261 ataaggaaaa aagggaggaa aaaagatgac atgctatctt aaagcagatt gtttttatta
     4321 cccaacagag gtgaaaccgg ctggctacct aagtcttcat gatggtgttt ttggggagtg
     4381 gacagaaatt gttccggcag atgcgcaaat cattgactac accggttatc aaattgcacc
     4441 aggtcttgtg gatacgcata tccatggcta tgcgggagct gatgtcatgg ataattctgc
     4501 ccaagggatt catcaaatga gtgaaggact attagcaaca ggagtaacgt cttttttacc
     4561 gacgacctta acttctacct ttgagcagtt agaaaaagta tcagggacta ttgcttcagt
     4621 cgctgatcaa gttaaagggg ctaagattca gggcatttac tttgaaggtc cttattttac
     4681 cgaagaatat aagggtgctc aaaacccaag ttatatgaaa aacccacgct tggaggaatt
     4741 tgatgcttgg caaaaagccg ctaaagggct catcaaaaaa attgcccttg ctcctgagcg
     4801 tgatggtgtc aaagaatttg tgtccgcagt gacaaaacaa ggcgtaacag tggccttagg
     4861 gcattcaaac ggtacttatc aagaggctaa agaagctgtt caagctggtg ctagtgtttg
     4921 ggttcatgct tataatggca tgcgaggatt gatccaccgt gaaccaggaa tggtgggagc
     4981 ggtttataat cttccaaata cttacgcaga gttgatttgt gatggccacc atgtctcacc
     5041 aattgcttgt gatattttga tgcagcaaaa agggcatgat catgtagcca tgattacgga
     5101 ctgtatgcga gcaggtggtt caccagatgg tgattacctc ttgggggaat tctctgtggt
     5161 tgtggcaaat ggcactgctc gtcttaaaga gagtggaaac ttggcaggat ctattttgaa
     5221 attaaaagac ggccttagaa atgtagtggc ctggggaatc gcaactccgg cagaagccat
     5281 tcatatggca acctatgtgc cagctgtttc tgtcggtatt gacgacgtat gtggtcaaat
     5341 caaagctggg catgcggctg actttattgt tcttgataag gatttaacac ttgttgcaac
     5401 ctatttaaat gggcaaaaag cttttgatgc ctaagagata caaaaggatt ggggtagcta
     5461 tgtctggcag gccaattctt tttgtatttt ctttctttat gatagaatag attacagtaa
     5521 tcgcttacag agattgataa aaggaggttt ttgtgatggt aacaacagta aaaatgacaa
     5581 gtggctatga gattcccgtt ttaggatttg gaacttacca agcagccgat ggtgaggaag
     5641 cttaccaatc taccttagca gccatcaagg ctggctatcg tcatattgac actgcagcta
     5701 tttataaaaa tgaagaaagt gtgggtcgtg ccatcaagga ttctggggtt ctacgagaag
     5761 acttatttat tacaactaaa ctctggaatg acgcccatag ttatgaaggg gcaaaagacg
     5821 ctttagctgc ttctcttggc cgtcttggtc tggcttatgt ggatctctat cttatccatt
     5881 ggccaaaccc aaaagctctg cgtaacactt ggaaagaggc aaatgcccaa gcatggcaat
     5941 atatggaaga ggcggttgaa gcagggctta ttaaaagcat tggcgtcagc aattttatgg
     6001 tgtatcatct agaagctctt caagagacag ctaaaataac tccagcaatt aatcagattc
     6061 gattagcccc aggttgctat caaaaggaag tggttgatta ttgtaaggca aatgagattt
     6121 tattggaagc ttggagtcct ctaggacaag gggagatttt tgataatgag actatgcagc
     6181 agttagctaa taaatatgac aaaacagtgg cacaagttgc cttagcttgg tcgctggcag
     6241 aaggttttat tcccttgcca aaatcggtgc atgatgagcg catcaaggaa aatatggcta
     6301 tttttgatgt gagcttgaca ccagaagata aaaaaacaat tcgctatctc tcaggaatgt
     6361 cagcgattcc taaccctgat actacaagtt tttaagaaac tacaaagatt tagccataat
     6421 tcgctaaatc ttttttgaaa acggtttgac aaaggtttat catttagcta taatctttat
     6481 tagtgttttg tgaaagaaac tattattgaa aataaggagc cgtattgtga ccatcttttt
     6541 agtgatgtta gccatgttta ctattcgttt agtatttttg aagaaatcga taacaaatga
     6601 aaaagccata ttagcccaag gaggacagga atttggtgct cagaacacga aatttttgac
     6661 gttacttcac attatgattt atgtgtttgc ggtgattgaa gcgctgctta aacaaattaa
     6721 gtttgatggc atcagttttt taggtctttt gttgatgtta ttatcagtag ctgttttata
     6781 tgaagtgaca cgtatcttag gtgacatttg gactgtaaaa ttgatgttag caaaagacca
     6841 caaatatgtg gatcactggt tgttcaaaac gattaaacat cctaactact ttttaaatat
     6901 cgcccctgag ctggtgggca tagccttact ttgccatgct aaaattacgg ctatgcttct
     6961 tttcccatgc tacattgtag taatatattt acgtattcga gaagaaaaca agctattagc
     7021 ggaagtgatt attccaaacg gtactcgaac aaaaccttaa gctcatgatg gacaaactaa
     7081 tggctttatg ctaaactaga ctctaggaaa aaggatgcaa gtatcttatc taaagcgagt
     7141 tcggggtagt tagagccgaa tggtaggact gcagattggc gcttccgttt gggcagtgtg
     7201 attaagtata tttgtcaata ttgcccaaaa agatactata taaacgaagt aataaattag
     7261 ggtggaaccg cgttttgacg cccctaggtc ttatgattta ggagcatcaa aatgcggttt
     7321 tttggtgcct agacctgtaa tacatgctag taacatcgtc aggtatttta ctgatgacaa
     7381 aaaacgattg tcaatgatat accattttac tactatcaaa ggagtacgaa atgtctaaaa
     7441 aacttacctt tcaagaaatc atcttgacct tacaacaata ttggaatgat cagggttgta
     7501 tgctcatgca ggcctatgac aatgaaaaag gagctgggac catgagtcct tacacgttct
     7561 tacgtgctat aggtcctgag ccatggaatg cggcttatgt agagccatca cgtcgtcctg
     7621 cagatggccg ttacggagaa aacccaaatc gtctttacca acaccaccaa tttcaagtgg
     7681 tcatgaaacc atcaccatca aatattcaag aactttacct tgcttctttg gaaaaattgg
     7741 gcatcaatcc tttagaacat gatattcgtt ttgttgaaga taactgggaa aacccctcaa
     7801 caggatcagc agggcttggt tgggaagttt ggcttgatgg aatggaaatc acacaattta
     7861 cgtatttcca acaagttggt ggccttgcga catctccagt gacagcagaa gtaacttatg
     7921 gtcttgagcg cttagcttcc tatattcaag aagtggattc ggtctatgat attgagtggg
     7981 caccaggtgt taaatatggt gaaatcttct tgcaacctga gtatgagcat tcaaaatatt
     8041 cctttgaaat gtcagaccaa gacatgctgc ttgaaaactt tgaaaagttt gaaaaagaag
     8101 cctcgcgtgc cctagaagaa ggtttggttc acccagctta tgattacgtg ctcaaatgtt
     8161 ctcatacgtt taatctgctg gatgcccgtg gcgctgtttc cgtcacagaa cgcgcaggct
     8221 acattgctcg tatccgtaat ttggcacgtg tcgttgctaa gacctttgtt gcagaacgca
     8281 agaaacttgg atttccactg ttggatgaag caacacgcgc aatcttgcta gctgaagatg
     8341 gcgaataagt cgatttaaag atgatctagt tgcgagcttt gtaatcttaa gagcgagcaa
//
LOCUS       DAUCLR010000081         8261 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.081, whole genome shotgun sequence.
ACCESSION   DAUCLR010000081 DAUCLR010000000
VERSION     DAUCLR010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8261
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.081"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            459..1175
                     /locus_tag="VGT87_001510"
     CDS             459..1175
                     /locus_tag="VGT87_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEQ3814347.1"
                     /translation="MGRKWANIVAKKTAKDGATSKVYAKFGVEIYVAAKQGEPDPELN
                     TALKFVIDRAKQAQVPKHVIDKAIDKAKGNTDETFVEGRYEGFGPNGSMIIVDTLTSN
                     VNRTAANVRTAYGKNGGNMGASGSVSYLFDKKGVIVFAGDDADSVFEQLLEADVDVDD
                     VEAEEGTITVYTAPTDLHKGIQALRDNGVEEFQVTELEMIPQSEVVLEGDDLETFEKL
                     IDALESDDDVQKVYHNVADF"
     gene            1378..2220
                     /locus_tag="VGT87_001511"
     CDS             1378..2220
                     /locus_tag="VGT87_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEQ3814348.1"
                     /translation="MIKKQYLGRATIALASTLVLAACGSSKTAESGNQGSSKEVLFAT
                     VGTTAPFSYEKGGQLTGYDIEVAKAVFKGSDDYKVSFKKTEWSSIFTGLDSGKYQMGG
                     NNISFTKERSAKYLFSYPIGSTPSVLVVPKDSDIKSFDDIQGHTTQVVQGTTSVAQLE
                     DFNKKHSDNPVTLKFTNENITQMLTNLSEGKADFKIFDAPTVNAIIKNQGLDNLKTIE
                     LTSTEQPFIYFIFSQDQEKLQSFVNKRIKELTADGTLSKLAKEHLGGDYVPSDKELKL
                     PTAN"
     gene            2547..3392
                     /locus_tag="VGT87_001512"
     CDS             2547..3392
                     /locus_tag="VGT87_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEQ3814349.1"
                     /translation="MSFKKWIGIVGIALASTVLVACSEKQDDKNTLTIGVMTKTESDQ
                     ARWDKVEELLKKDNITLKYKEFTDYSQPNKAVANGEVDINAFQHYNFLNNWNKENKEH
                     LVAIADTYISPINLFSGTSQDGKAKYKSVADLPNGAQIAVPNDATNESRALYVLQSAG
                     LIKLNVSGDQLATIANISENKKKLDIKELDASQTARALVSADAAVVNNSYAVPAKIDY
                     KTSLFKEKADDNSKQWINIIAGQKDWEKSEKADAIKKLIKAYQTDEVKKVVEKTSNGI
                     DVSVW"
     gene            3642..4706
                     /locus_tag="VGT87_001513"
     CDS             3642..4706
                     /locus_tag="VGT87_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEQ3814350.1"
                     /translation="MNEAIIQLDHIDITFRQKKRVIEAVKDVTVHINQGDIYGIVGYS
                     GAGKSTLVRVINLLQAPTNGKITVDGDVTFEQGKVQLSANALRQKRRDIGMIFQHFNL
                     MAQKTAKENVAFALRHSSLSKTEKEHKVIELLELVGLSERADNYPAQLSGGQKQRVAI
                     ARALANDPKILISDEATSALDPKTTKQILALLQELNRKLGLTIVMITHEMQIVKDICN
                     RVAVMQNGVLIEEGSVLDIFSNPKEALTQEFITTATGIDEALEKINQQDIVKHLPANA
                     LLAQLKYAGTSTDEPLLNSIYRQFEVTANILYGNIEILDHIPVGDMIVVLEGQAENIL
                     AAEKALHEAGVDVSILKRGA"
     gene            4707..5399
                     /locus_tag="VGT87_001514"
     CDS             4707..5399
                     /locus_tag="VGT87_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ3814351.1"
                     /translation="MSQLIQTYLPNVYELGWSGDAGWGLAIWNTLYMTIVPFIVGGAI
                     GLLLGLLLVLTGPDGVIENKTICWVIDKVTSIFRAIPFVILIAILASFTYLLLRTTLG
                     ATAALVPLTFATFPFYARQVQVVFSELDKGVIEAAQASGATFWDIVKVYLSEGLPDLI
                     RVSTVTLISLVGETAMAGAIGAGGLGNVAISYGYNRFNNDVTWVATIIILLIIFAIQF
                     IGDSLTRRFSHK"
     gene            complement(5453..6823)
                     /gene="brnQ"
                     /locus_tag="VGT87_001515"
     CDS             complement(5453..6823)
                     /gene="brnQ"
                     /locus_tag="VGT87_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEQ3814352.1"
                     /translation="MKQKNVYIVIGFMLFALFFGAANLIYPAFLGIYSGHQILWSIIG
                     FCLTGVSLPLLGVIAVAKSGSGDVESLARPISKWYAIFYSSILYLSIGPFFAIPRTGA
                     TSFSVGIAPILGDNTTNKAIYAILFFGLSYFLAIKPSKLAENIGKFLTPTLLVVISIL
                     VITSFVHPAGNYGDAFNAGVGVNNAFKDFPFIAGLIQGYGTMDALASLVFAILVIEAT
                     KQFGAKTDKEVTKITLISGAIAILLLALVYIFVGRIGATSQSLFPFSDGSFTLHGNPV
                     NGGQILSHASRFYLGGIGQSFLAVVIFLACLTTSTGLITSSAEYFHKLVPTLSHIAWA
                     TIFTLLSAFFYFGGLSVIINWSAPVLFLLYPLTVDLIFLVLAQKCFNNDPIVYRTTIG
                     LTFIPAIFDALLTLSQMTGLFHLPEAVVTFFQKTVPLGQFSMGWIIFAAIGFLIGLIL
                     SKTKKS"
     gene            7057..>8261
                     /gene="sstT"
                     /locus_tag="VGT87_001516"
     CDS             7057..>8261
                     /gene="sstT"
                     /locus_tag="VGT87_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012516260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEQ3814353.1"
                     /translation="MKKIYDLWVRVSLIKKIGIGVVIGVMLGILAPDLTGFSILGKLF
                     VGGLKAIAPLLVFALVSQAISHQKKGRQTNMTLIIVLYLFGTFASALVAVLTAYLFPL
                     TLVLNTPVNTELSPPQGVAEVFQSLLLKLVDNPINALATANYIGVLSWAIIFGLALKA
                     ASQETKHLIKTAAEVTSQIVVWIINLAPIGIMSLVFTTISENGVGILSDYAFLILVLV
                     GTMVFVALVVNPLIAVLITRQNPYPLVLRCLRESGLTAFFTRSSAANIPVNMQLCQKI
                     GLSKDTYSVSIPLGATINMGGAAITINVLTLAAVHTFGIPIDFLTALLLSVVAAVSAC
                     GASGVAGGSLLLIPVACSLFGISNDLAMQVVGVGFIVGVIQDSCETALNSSTDVLFTA
                     IAENAFWKR"
BASE COUNT         2743 a         1329 c         1665 g         2524 t
ORIGIN      
        1 gcttgactct aacattacgt tgatggttta tacttcagtt aatccaatga ggaaatttta
       61 taacaataat taaaaaagtc agtcaaaaga tatgctttcg aagaacaggc taacgttttt
      121 acaagattat atcagtaatc attttgacaa ttgtagtaat ggaatgttgc caaaatttag
      181 aacgttgtgt gttatgaatg acaagatttt aaaggaatac taattaaacg ggaggtcgtg
      241 aaccggaagc tgttgtcact caagcgccaa aatctgtctg taacataata ttgatgtatg
      301 ggaacttaat tatttggttt ttagtgtgat gcttaaaacg attttatatt tctattttac
      361 caaagctttt caaagtgcta taataaggga gttagagtca atattagtaa cgttaatgtt
      421 gacaatattc aataaaatac tattaaaagg agacaaaaat gggacgtaaa tgggcaaata
      481 ttgttgctaa aaaaactgct aaagatggag caacatcaaa ggtctatgcc aaatttggtg
      541 ttgaaattta tgtggctgcc aaacaaggcg agccagatcc agagttaaat acagctctta
      601 aatttgtcat tgatcgtgca aagcaagcac aagtgccaaa gcatgttatt gataaagcga
      661 ttgataaagc taaaggaaac acagatgaaa ctttcgtaga gggacgctat gaaggttttg
      721 gtccaaatgg ttcaatgatt attgtggata ctttgacatc aaatgttaac cgtacagcgg
      781 caaatgtacg tacggcttac ggtaagaacg gtggcaatat gggagcttca ggatctgtat
      841 cctacttatt tgataaaaaa ggggtcatcg tttttgctgg tgatgatgct gacagtgtct
      901 ttgaacaatt acttgaagcg gatgtagacg tagatgatgt tgaagcagaa gagggaacaa
      961 taacagttta cactgcccca acagatcttc ataaaggtat ccaagcactt cgcgataatg
     1021 gtgtagaaga attccaagtt actgaacttg aaatgattcc tcaatcagaa gtagtattgg
     1081 aaggtgatga ccttgaaact tttgaaaagc ttattgatgc actcgaatca gatgatgatg
     1141 ttcaaaaagt gtatcataat gtagcagatt tttaaattga catagaataa taaaaagagt
     1201 tgaaatctat ttcagctctt tttaagtgat gcttttataa gtaagtacca ggtaacatag
     1261 tcgttgttag aagataatga acaatagtaa atcagtatca acttgatagg aatcaattat
     1321 tagtgaactc taattaaaaa tggtaaacta gacacaagaa caaaaaggag agataggatg
     1381 attaaaaaac aatatttagg aagagcaaca attgccctag catcaaccct tgtgctagca
     1441 gcttgtgggt catcaaaaac agctgagtca ggaaatcagg ggagttctaa agaagtctta
     1501 tttgcaacgg ttggaacaac agctcccttt tcttatgaaa aaggtggtca actaactggg
     1561 tacgatatcg aggtggctaa agcagtcttt aaaggatcgg atgattacaa ggtgtccttt
     1621 aaaaaaacag aatggtcttc tatctttaca ggtttggact caggaaaata ccaaatgggt
     1681 ggtaacaaca tttcctttac caaagagcgt tcagctaagt accttttttc ttatccaatc
     1741 ggatcaacac cttctgtttt agttgttcca aaagacagtg atatcaagag ctttgatgat
     1801 atccaagggc atacgacaca agttgttcaa gggactacaa gtgttgcaca gttagaagac
     1861 tttaataaga aacacagtga caacccggtt actctaaaat tcaccaatga gaacattact
     1921 caaatgttaa ccaatttgag tgaaggcaag gcagatttca agatttttga cgctccaacg
     1981 gttaacgcca ttattaaaaa tcaagggtta gataatctca aaaccattga actcacctca
     2041 acagagcaac catttattta ttttatcttt agtcaagatc aagagaaatt gcaaagcttt
     2101 gttaacaagc gtattaaaga gttgactgct gacggaactc ttagcaagct tgccaaagag
     2161 caccttggcg gtgattacgt gccatcagat aaagagttga aacttcctac agctaactaa
     2221 accatctatt aaccagttat tcatctagaa gactggtttt tctgatcgga aacaatatta
     2281 tagtttatga ggttatgtta taaaaattgc aaatagcctt aatcaaaatg agatgttaaa
     2341 gtgctttttg agaagtgaat attgtaagaa gtatgctttg ggttttagtc ctcaaatacc
     2401 aagttttttg ttgactaaac aaataaggat ggttagcata gcatatagtt taaaactatg
     2461 gtatatgcta gccattatca atttgaaaac tatatctaat taagatacaa taatgatcgt
     2521 aaacaaaatg aatcgaggat aacgctatgt cttttaaaaa atggattgga atagtcggaa
     2581 tcgctttggc atcaactgta ttggttgctt gttctgaaaa acaagatgat aaaaacactc
     2641 taactattgg ggtgatgaca aaaacagaat ctgatcaagc gagatgggat aaagttgaag
     2701 aattattaaa aaaagacaat atcactttga agtacaaaga atttacagat tactcgcaac
     2761 ctaataaggc agttgctaat ggtgaagtcg atattaatgc cttccaacac tataatttct
     2821 taaataactg gaataaggaa aataaggaac atttggtggc cattgctgat acctatatca
     2881 gcccaattaa ccttttctct ggaaccagtc aagatgggaa ggctaaatac aaatcagtag
     2941 ctgatctgcc aaatggtgct caaattgcag taccaaatga tgcgaccaat gaaagccgtg
     3001 ccctctatgt gctgcaatca gctggcctca ttaaattgaa tgtgtctggt gatcaattag
     3061 caaccattgc caatatctca gagaacaaaa agaaattaga tatcaaggaa ttagatgcta
     3121 gccaaacggc acgtgcttta gtatctgctg atgcagctgt tgtcaataat agttacgctg
     3181 ttcctgcaaa aattgactac aaaacctctc ttttcaaaga aaaagcagat gacaattcaa
     3241 aacaatggat taacattatt gcaggtcaaa aagattggga aaaatcagag aaggctgatg
     3301 ctattaaaaa actcatcaag gcctaccaaa ctgatgaggt gaagaaagtc gtggaaaaaa
     3361 cttcaaatgg tatagatgta tccgtatggt aaattttagc cattgttata acaagagtgt
     3421 ggttgtgttt aaaacacatg ctttcttgca tgcttgagga agagtttacg attaacttac
     3481 ctttagaaag ctatttaaga ccttttccct tttaggattt aggaaccttg ggaagactat
     3541 tgcatgttat cttaatagtc atgattatgc aggcgatgaa aacattgcta tcgtagatta
     3601 ctgtcgccat atcgtactaa tagaggagtt attaaaatct tatgaatgaa gcaataattc
     3661 aattagatca tattgatatt accttccgtc aaaaaaaacg ggttatcgaa gctgttaaag
     3721 atgtcacggt tcatatcaat caaggagata tttatggcat tgttgggtat tcaggtgctg
     3781 ggaaatcaac tcttgtgcgt gtgattaacc tgctacaggc accaacaaac gggaaaatca
     3841 ctgttgatgg ggatgttacc tttgaacagg ggaaagttca gttatcagcc aatgcccttc
     3901 gtcaaaaacg acgtgatatt ggcatgatct tccaacattt caatttgatg gcccaaaaaa
     3961 cagccaaaga aaatgtagca tttgcgctcc gccattcatc gttaagtaag actgagaaag
     4021 aacataaagt tatcgagctg ctagaacttg taggtttatc agagcgagca gataactacc
     4081 ctgcccagtt atcgggtggg caaaaacaaa gggtggctat tgcgcgtgct ctagctaacg
     4141 atccaaaaat tttgatttca gatgaggcca catcagcact agatccaaaa accactaaac
     4201 aaattttggc gctcttgcaa gaattaaacc gcaaacttgg ccttaccatt gttatgatta
     4261 ctcatgaaat gcaaattgtt aaggatattt gtaatcgtgt tgctgttatg caaaatggtg
     4321 ttttgattga agaaggttct gtccttgata tcttttcaaa tccaaaagaa gccttgacac
     4381 aggaatttat cacaacagct acagggattg atgaggcttt agaaaaaatt aaccaacaag
     4441 acattgttaa acatttgcct gcaaatgccc ttctagctca gttgaaatat gcaggaactt
     4501 ctacggatga gcctctactt aatagcattt accgtcaatt tgaagtgaca gctaatattt
     4561 tgtatggcaa tattgagatt ttagatcata ttccagttgg tgacatgatt gttgtcttgg
     4621 aagggcaagc tgaaaatatt cttgcggctg agaaagcctt gcatgaagct ggtgttgatg
     4681 ttagtatttt aaagagagga gcctagatgt cacagttaat tcaaacttat ttgccaaatg
     4741 tttatgaact cggatggtct ggagatgctg gttggggctt agcgatttgg aatactttat
     4801 acatgaccat tgtgcctttt attgttgggg gtgctattgg tctcttatta ggcttactat
     4861 tagttttaac aggaccggac ggtgttattg aaaacaaaac gatttgttgg gttattgata
     4921 aagttacctc tatttttaga gccattcctt ttgttatttt gattgctatt ttagctagct
     4981 ttacctattt gcttttaaga acaactttag gggcaacagc ggccttggta cctttaacct
     5041 ttgcgacttt tcctttttat gcccgtcagg tccaagttgt tttttcagaa ttagacaagg
     5101 gtgttattga agcagcgcaa gcttcaggtg ctactttctg ggatattgtc aaagtgtatc
     5161 tcagtgaagg tttaccagat cttattcgtg tttctacagt caccttaatt tctttagttg
     5221 gggaaactgc catggcagga gctatcggtg ctggaggatt aggaaatgta gcgatttctt
     5281 atggttataa tcgttttaac aatgatgtga cttgggtagc gactattatt attcttttaa
     5341 ttatttttgc tatccaattt attggtgata gcttgactag aaggtttagt cataaataaa
     5401 cgaaacaaca ccctaagttt ttacttaggg tgttgttgtt tagatgatgt cattagcttt
     5461 tcttcgtttt acttagtata agccctatta aaaaaccaat agcagcaaag ataatccatc
     5521 ccattgagaa ttgccctagt ggaacagttt tttggaaaaa agttacaacg gcttctggta
     5581 aatgaaataa tccagtcatt tgtgatagtg ttaggagtgc atcaaatatg gcaggaataa
     5641 aggttagacc aattgtagtt cgatagacaa taggatcatt attgaagcat ttttgtgcca
     5701 aaacaaggaa aattaaatcg actgttaatg ggtataaaag gaataaaaca ggagctgacc
     5761 agttgataat gactgataag ccaccaaaat aaaagaaagc tgatagtaaa gtaaagatag
     5821 ttgcccaagc aatatgagat aaagtaggca ctaatttatg gaagtattca gcacttgacg
     5881 tgattaagcc tgttgaagtg gttagacagg ccaggaaaat cacaacagct agaaatgatt
     5941 gtccgatgcc acctaggtaa aaacgagaag catgacttaa gatttgaccg ccattaactg
     6001 gattaccatg aagggtaaag ctgccatcac taaaaggaaa taatgattgt gatgttgctc
     6061 caatacgacc gacaaagata tagacaagtg ctaatagcaa aatggcaata gccccagaaa
     6121 taagtgttat tttggtcact tctttgtccg tcttagcgcc aaattgtttg gtagcctcaa
     6181 tgactaaaat agcaaaaaca agagaagcta gtgcatccat agtgccataa ccttgaatta
     6241 atcctgctat aaaaggaaaa tctttaaagg cattattaac accgacccca gcgttaaaag
     6301 catcaccgta atttccagca ggatggacaa aggacgtgat aaccaaaata gaaataacaa
     6361 ctaacaacgt tggcgttaaa aattttccga tattttcagc tagcttacta ggtttgatag
     6421 caaggaagta ggacagacca aaaaataata tagcataaat agctttattg gttgtattat
     6481 ctcctaagat aggagcgata ccgactgaaa aagaagtggc tcctgttctt ggaatagcaa
     6541 aaaatggacc aatagataaa tacaaaatgg aagaatagaa gattgcatac catttagaga
     6601 tggggcgtgc caaactttca acatcgcctg atccagattt agcaacagca atgacaccga
     6661 gtaaaggcaa ggagacacca gttaaacaaa aaccaatgat agaccataga atctgatgac
     6721 cggagtagat gcctaaaaaa gctgggtaaa tgaggtttgc tgctccaaaa aatagtgcaa
     6781 atagcatgaa tccaatgacg atatatacat ttttttgttt catttataaa tagtctttct
     6841 attgatacta atccgaagat tctaacaatt ttaattatat tatgtgtcaa aataacaaca
     6901 aaggttatta tatctaagat tacttcgata gtcaatagaa attgactgaa aattcttagc
     6961 acaaaaaatg gttaagatat gtagcttatt cgatttgacg aatagataga attatattat
     7021 tataaaaaac atgtaacttt agtaaggatg taacgtatga aaaaaatata tgatttatgg
     7081 gttagggtta gtctaatcaa aaaaataggt atcggtgttg taataggagt catgttgggc
     7141 attttagctc ctgatcttac aggttttagt attttaggaa agttatttgt tggtggttta
     7201 aaggctatcg cacctttgct tgtatttgct ttggtttccc aagccatctc ccatcagaaa
     7261 aaagggagac agactaatat gacattaatc attgttctgt atttatttgg tacctttgcg
     7321 tcagctttag tagcagtact gacagcttat ctattccctt tgacgttagt gttaaataca
     7381 cctgtaaata cagaattatc accacctcaa ggtgttgctg aagtttttca gtcacttcta
     7441 ttaaaattag tggataatcc gatcaatgct ttagctactg ccaattatat tggtgtgcta
     7501 tcatgggcaa ttatttttgg actagcatta aaggctgcaa gtcaagaaac aaagcatctt
     7561 ataaaaacag cggcagaagt tacttcacaa atagttgttt ggatcatcaa tctggcacct
     7621 attgggatta tgagtctggt gttcactacg atttctgaaa acggtgttgg aatcttatct
     7681 gattatgctt ttttgatact agttttagta ggaacaatgg tttttgtcgc cttagtggtt
     7741 aatccactta ttgcggtttt gattacacga caaaatcctt atccccttgt cttgagatgt
     7801 ttacgtgagt caggtcttac agcatttttc acacgaagtt ctgcagccaa tattccagtc
     7861 aatatgcaat tatgccaaaa aattggattg agtaaagata cttattcagt gtcaattcca
     7921 cttggagcaa ctattaatat gggtggggca gcaattacca tcaatgtcct aacgcttgct
     7981 gctgtacaca cttttggtat tcctattgat tttctaacag ccctcttact tagcgttgtt
     8041 gctgctgtct ctgcttgtgg tgcttctggt gttgctgggg gatcgttgtt acttattcct
     8101 gtagcatgta gtttgtttgg catttcaaat gatcttgcga tgcaggtggt tggtgttggt
     8161 tttattgttg gtgttatcca ggattcttgt gagacagcct taaattcgtc aaccgatgtt
     8221 ctttttacag ctattgctga aaatgctttt tggaaacgta a
//
LOCUS       DAUCLR010000082         8176 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.082, whole genome shotgun sequence.
ACCESSION   DAUCLR010000082 DAUCLR010000000
VERSION     DAUCLR010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8176
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.082"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(9..851)
                     /locus_tag="VGT87_001517"
     CDS             complement(9..851)
                     /locus_tag="VGT87_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEQ3814354.1"
                     /translation="MEIGETVEFIRHSKNISIKQVCGDYLTRQTYYRFIKNNLDISSK
                     KLLYILDNLNVNVDEFLFISNNFKQYKEFIDMDTAKHYFECRNIEGLNHILDSYKDSK
                     STKEKNLFALVKVLLATLTEEDCLTERTYLSNYLINIETWSHYETVLFNNCMFIFESC
                     FIEMVFSKVILNLDKYNTLRYYGNESIRMFVNMLILFIQRQEYDKASEILAKIEDYQL
                     NDDCLYERCCVSFFDGIIGLINGKEGAEQKCVQILEIFQLLNCKTIHHMFQTYLEAIK
                     HKLS"
     gene            1350..1520
                     /locus_tag="VGT87_001518"
     CDS             1350..1520
                     /locus_tag="VGT87_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814355.1"
                     /translation="MDNPKKDKGLSLGVVDPITILITESVINKMRTVPYHLKKLSSTT
                     YCLLADLAYQSA"
     gene            1792..2988
                     /gene="speB"
                     /locus_tag="VGT87_001519"
     CDS             1792..2988
                     /gene="speB"
                     /locus_tag="VGT87_001519"
                     /EC_number="3.4.22.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine proteinase exotoxin SpeB"
                     /protein_id="HEQ3814356.1"
                     /translation="MNKKKLGIRLLSLLALGGFVLANPVFADQNFARNEKEAKDSAIT
                     FIQKSAAIKAGARSAEDIKLDKVNLGGELSGSNMYVYNISTGGFVIVSGDKRSPEILG
                     YSTSGSFDANGKENIASFMESYVEQIKENKKLDTTYAGTAEIKQPVVKSLLDSKGIHY
                     NQGNPYNLLTPVIEKVKPGEQSFVGQHAATGCVATATAQIMKYHNYPNKGLKDYTYTL
                     SSNNPYFNHPKNLFAAISTRQYNWNNILPTYSGRESNVQKMAISELMADVGISVDMDY
                     GPSSGSAGSSRVQRALKENFGYNQSVHQINRSDFSKQDWEAQIDKELSQNQPVYYQGV
                     GKVGGHAFVIDGADGRNFYHVNWGWGGVSDGFFRLDALNPSALGTGGGAGGFNGYQSA
                     VVGIKP"
     gene            2990..3295
                     /locus_tag="VGT87_001520"
     CDS             2990..3295
                     /locus_tag="VGT87_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spi family protease inhibitor"
                     /protein_id="HEQ3814357.1"
                     /translation="MEMHFVRTEPEARRIAETFCAENTQTKTPMRVQQLSYPSDTDHS
                     GGELYIYALSPAGFIIVSGDTRAHTILGYSFDNNLDLNHDNVRSMIEAYQTQISSLD"
     gene            3780..4709
                     /gene="prsA"
                     /locus_tag="VGT87_001521"
     CDS             3780..4709
                     /gene="prsA"
                     /locus_tag="VGT87_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEQ3814358.1"
                     /translation="MKQMNKLITGVVTLATVVTLSACQSSHNNTKLVSMKGDTITVSD
                     FYNETKNTELAQKAMLSLVISRVFETQYANKVSDKEVEKAYKQTADQYGTSFKTVLAQ
                     SGLTPETYKKQIRLTKLVEYAVKEQAKNETISKKDYRQAYDAYTPTMTAEIMQFEKEE
                     DAKAALEAVKAEGADFAAIAKEKTTAADKKTTYTFDSGETTLPAEVVRAASGLKEGNR
                     SEIITALDPATSKRTYHIIKVTKKATKKADWKAYQKRLKDIIVTGKLKDPDFQNKVIA
                     KALDKANVKIKDKAFANILAQFAKPNQKQPAPK"
     gene            complement(4986..5222)
                     /locus_tag="VGT87_001522"
     CDS             complement(4986..5222)
                     /locus_tag="VGT87_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814359.1"
                     /translation="MTHRFFLTPLSTLWRRLRPKRYSKKVLADEPTSYQPPTAHGPCD
                     DTHQLLEDKSLSDDYLISTSAMRHLWYWGYSQIG"
     gene            5646..>8176
                     /locus_tag="VGT87_001523"
     CDS             5646..>8176
                     /locus_tag="VGT87_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HEQ3814360.1"
                     /translation="MTNCKYKLRKLSVGLVSVGTMLIAPTVLGQEVSVSTETSASTST
                     STASAGTSTSETAASGTASGTNGASSVTSSEGSQSSESAPASPQPQAQTAVASSSSSS
                     NANASSSSDGKAPQAASTTSSSTPAAASSNGISQGTSAEAETPMMEVEQYTVGNKTTE
                     LNIKDGAQLKNGSKVDKDTKLIRNRDGEQRDIVDIKREVKTNADGTVDVTVTVTPKEI
                     DKGADVMALLDVSKKMTEDDFNNAKDKIKKLVTTLTSKSPDSQPNHNARNSVRLMTFY
                     RKVNDPIELNADNVDAKLDEVWKKAKEDWDWGVDLQGAIHKAREIFNKGKRKKSGKRQ
                     HIVLFSQGESTFSYDINDKDENDTVQKNRIAGKVTTSNPLFPWLPIFNHTNQKAEVID
                     DVDKLLGLAEKMGISLPDGLRAGVKAIGAGSSFLSTFTGSGLTEYLTLNEYRSDILKE
                     KQFDYTKRVGEGYYYHSYSKRTHGDKMPFEKQIREVLERFLPKIEDKEWPKKFIEIFG
                     LQEKEVNQIGVDVIMKVINSIFYKREYHYYNHNLSAIAEAKMAQKEGITFYSVDVTDS
                     DKASKRVKRQVGNEQSKKKKEDAEKDRNNKFDDYLKKMSEGGNFLSKVEERGKFKDTL
                     TELTIKDEFTNKVKVQEDSEGKKYKTGLISGTSNVTHTPAKTGFFSSNESLTWTISKE
                     QLKKAFEDGTSLTLTYKLKVENDKFKKSLEEDNKKKRAKRSAPTENENSVTEKIISNT
                     TTYKINEQKVEGNKFDDVNLTYSKFKVPVPQIDGQVIEPQAPKLPDLPPVIEHGPVLD
                     YTEESIYRLPLEHGSNAPDTQVTIEEDTVPQRPDILVGGQSGPVD"
BASE COUNT         2876 a         1634 c         1562 g         2104 t
ORIGIN      
        1 aaagagcctc aggacagttt atgtttaatg gcttctaggt aggtttgaaa catatgatgg
       61 atcgttttgc aattaagtaa ttggaagatc tccaagatct gcacacactt ttgctcagcc
      121 ccctctttac catttatgag gccaataatg ccatcaaaaa aagacacaca acaccgttca
      181 tataagcaat catcatttag ctgataatct tcaatttttg ccaaaatctc agaagcttta
      241 tcatactctt gtcgctgaat aaacaaaatc aacatattga caaacatccg aatcgattca
      301 ttcccataat accttagggt attgtattta tcgaggttca aaataacttt tgaaaacacc
      361 atctcaataa agcaagactc aaaaataaac atacaattat taaaaagcac agtctcatag
      421 tgactccaag tttcaatatt aataagatag tttgacaaat aagtccgctc tgtcagacag
      481 tcttcctcag taagagttgc taataacacc ttgaccaagg caaaaaggtt cttttccttt
      541 gttgacttac tatctttata agaatcaagg atatgattta aaccttctat gtttcggcat
      601 tcaaaataat gttttgccgt atccatatca ataaattctt tgtattgttt aaagttatta
      661 ctaataaaga gaaactcatc aacattgaca ttcaagttgt ctaagatata aagtaacttt
      721 ttcgaagaaa tgtcaagatt atttttaata aaacgataat aggtttgcct agtgagataa
      781 tcaccacaaa cttgtttaat cgaaatgttt tttgaatgcc taatgaattc aacggtttca
      841 ccaatttcca tatgtcaagc cttcctagtt gatgtcaaaa atacgttacg catgtaccta
      901 atacgtaaca agttgaatgt ttcggatgat agtcgcttat gataggtgca taaggtcaat
      961 agccagatgc gatagtttta tcaactgtca tatgttaaac ctttctaatc taatagatgt
     1021 taaaaatacg ttacgtgtgt acctaatacg taacaaaata atgggttagc aaaataagca
     1081 gctatgatat agccataagg ttaaaaggag gcgcctacta tgtggttatt gttactattt
     1141 ttgtagttcc ttttgcaaaa tggtagtaga taaaaatgag aagtcaataa aagcattgac
     1201 aaacaaaaga atcattttgt ttatattatc agtattttta agtttcttgt caactgaaat
     1261 gagcatctac tagccacaat agtaactaaa aggctaattg tcagtaaaaa tgagataaat
     1321 cagccaggaa aaagcctacg agtcaactaa tggacaaccc aaaaaaagac aaaggacttt
     1381 cacttggtgt tgtggaccct atcacgatcc ttatcacaga atcagttatt aacaaaatga
     1441 gaacggtccc atatcatcta aaaaaactgt caagcacaac ctattgtttg ctagctgatc
     1501 ttgcctatca gtctgcctag tagatacggc agaagataag agatcaacca aaagatgata
     1561 agtgcaacga ctcatagcgt cttttgacca aaaccaatta ggctcacatg agatgagaat
     1621 aattgggttg ggttgtcagt gtcaactaac cgtgttattg tctattacca ttcatggtat
     1681 cagcgacatc gtatgataac catacgattc agctaagtaa ggaggtgtgt ccaatgtacc
     1741 gttaaaagca aatgcagtag attaacttat tttgaaagag gtataaaaaa aatgaataaa
     1801 aagaaattag gtatcagatt attaagtctt ttagcattag gtggatttgt tcttgctaac
     1861 ccagtatttg ccgatcaaaa ctttgctcgt aacgaaaaag aagcaaaaga tagcgctatc
     1921 acatttatcc aaaaatcagc agctatcaaa gcaggtgcac gaagcgcaga agatattaag
     1981 cttgacaaag ttaacctagg tggagaactt tctggctcta atatgtatgt ttacaatatt
     2041 tctactggag gatttgttat cgtttcagga gataaacgtt ctccagaaat tctaggatac
     2101 tctaccagcg gatcatttga cgctaacggt aaagaaaaca ttgcttcctt catggaaagt
     2161 tatgtcgaac aaatcaaaga aaacaaaaaa ttagacacta cttatgctgg taccgctgag
     2221 attaaacaac cagttgttaa atctctcctt gattcaaaag gcattcatta caatcaaggt
     2281 aacccttaca acctattgac acctgttatt gaaaaagtaa aaccaggtga acaatctttt
     2341 gtaggtcaac atgcagctac aggatgtgtt gctactgcaa ctgctcaaat tatgaaatat
     2401 cataattacc ctaacaaagg gttgaaagac tacacttaca cactaagctc aaataaccca
     2461 tatttcaacc atcctaagaa cttgtttgca gctatctcta ctagacaata caactggaac
     2521 aacatcctac ctacttatag cggaagagaa tctaacgttc aaaaaatggc gatttcagaa
     2581 ttgatggctg atgttggtat ttcagtagac atggattatg gtccatctag tggttctgca
     2641 ggtagctctc gtgttcaaag agccttgaaa gaaaactttg gctacaacca atctgttcac
     2701 caaatcaacc gtagcgactt tagcaaacaa gattgggaag cacaaattga caaagaatta
     2761 tctcaaaacc aaccagtata ctaccaaggc gtcggtaaag taggcggaca tgcctttgtt
     2821 atcgatggtg ctgacggacg taacttctac catgttaact ggggttgggg tggagtctct
     2881 gacggcttct tccgtcttga cgcactaaac ccttcagctc ttggtactgg tggcggcgca
     2941 ggcggcttca acggttacca aagtgctgtt gtaggcatca aaccttagta tggaaatgca
     3001 tttcgttaga acagaacctg aggcacgccg catagctgaa accttttgtg ctgaaaacac
     3061 acaaacaaaa acccctatgc gcgtgcagca gttaagctat ccatcagaca cagatcactc
     3121 aggtggtgag ctatacatct atgctttgtc tcctgctgga tttatcatcg tatcaggaga
     3181 caccagagcg cacaccattt taggctattc ttttgataat aacctagacc tcaaccatga
     3241 taatgtcagg agtatgatag aagcttacca aacacaaatc agctctttgg attgatcggt
     3301 caaagtagct tttgccccct caccactcgt ttaagacgac cgtgtttcta aaataccact
     3361 accttttaag tttcacgccg gcctaagcga gtgtttgtct catggagcaa actgtttcca
     3421 tcgaaaagtc acccattgat aaaggaggtc atcaacgcct actaatgatg acttccctaa
     3481 gatgccaagg taaaggactg agttctgtag agatgggact cagtcctttt acgatttttg
     3541 ggagttattc tagccatacc gtggcaagaa aacaacactc aaaaatagcc aacgtcagtt
     3601 tctgttcata catgaccaca gccaaacacc acaacctaac ctaacctatc ctaaacacag
     3661 tggctttcta acgaacagtc acaacgagca gcaacaccaa aaagcaagat taagctacgt
     3721 ttaagactaa tgatataaga taacagtaat tattaatgag aaaagaggct caaaaggaca
     3781 tgaaacaaat gaataaactc attacaggag tggtaacgct ggcgacagtc gtgaccttat
     3841 cagcttgtca atcatcacac aacaacacca aactcgtctc gatgaaagga gacaccatca
     3901 ctgtcagtga cttctacaat gagaccaaaa acacagaact cgcacaaaaa gccatgttaa
     3961 gcttggtgat tagccgcgtt tttgagacac aatatgccaa caaagtctct gacaaagagg
     4021 ttgaaaaagc ctataaacaa accgcagacc aatacggtac atcctttaag acagtcctag
     4081 cacaatcagg cttaacacca gaaacctata aaaaacaaat tcgcctcaca aaattagtcg
     4141 aatatgccgt caaagaacaa gccaaaaacg aaaccatctc aaaaaaagac taccgtcagg
     4201 cctatgacgc ttatacccca accatgaccg cagaaatcat gcagtttgaa aaagaagagg
     4261 atgccaaagc agcacttgaa gccgtcaaag ctgaaggggc agactttgca gctattgcca
     4321 aagaaaaaac cactgcagcc gataaaaaaa caacctacac gtttgactca ggtgaaacaa
     4381 ccctaccagc agaagtagtt agagctgcat caggcctcaa agaagggaac agatcagaaa
     4441 tcatcacagc gcttgatcca gccacctcaa aacgcaccta ccatatcatc aaagtcacca
     4501 aaaaagcaac taaaaaagca gactggaaag cgtaccaaaa acgcttgaaa gacatcatcg
     4561 tgactggcaa attaaaagac cctgacttcc aaaacaaagt catcgctaaa gctcttgata
     4621 aagcaaatgt caaaatcaaa gacaaagcat ttgccaatat cttagcccag tttgcaaaac
     4681 ctaaccaaaa acaacctgcc ccaaaataga gggttgtcag tttatttccc taaggttgct
     4741 taaaaagata gccctagcca tcactcccct tttcaaaaca ccgataatgg aaacgtgtcc
     4801 tatactgatg gtctagggca atgtcataaa gctaaagact ctatccaaaa ataaggatag
     4861 agtctttaat gttttaaaac cagtcagcaa aaaaattcac gctaagccta gctagaatga
     4921 ggcttagcgt gaatggtatc agtgatagtc cgttaaacta gctgattggg agtcagaaac
     4981 tctccttacc caatctgact atacccccag taccagaggt gtctcatcgc agaagtagaa
     5041 atcagataat cgtctgataa gctcttgtcc tcaagcaact gatgagtatc gtcgcacggg
     5101 ccatgagctg tcggtggctg atagctagta ggctcatcag ccaaaacttt tttggagtaa
     5161 cgctttggtc tcagacggcg ccagagggtt gagagagggg tgagaaagaa acgatgggtc
     5221 atcttggcct ccttatctaa tgatagtcac cttaacagac aagagtgtca atactgtcat
     5281 tttaaggaga aaaagattaa aaatcaaggt cacaaacttt cctttgctga aaaaacgcga
     5341 ccataataaa gaacctaaat acccctcgta cttgatcttc tctttgcgca gttatcttgc
     5401 gcactcagtt tcctccaaga tagtgatagc gataccatta ctgacctata ggctatcgcg
     5461 gcaacccctt ttttcgcctc aatgaggctg atacagctgt catgacagga aaaagacctc
     5521 tgcctatcta agggatcatt aatttttttc tcaccaaaaa actgatttta gaaacgaaaa
     5581 agcatggtgt ataataaggt tcggaacaat tatgacataa taatgaaagt aaggttaacg
     5641 aaacattgac aaattgtaag tataaactta gaaagctatc tgtagggctc gtctccgtcg
     5701 gaacgatgct gatagccccg acagttttag gacaggaggt tagtgttagt actgagacga
     5761 gtgctagtac tagtactagt accgctagcg ctggtaccag tacgagtgag acagctgcca
     5821 gcggaactgc gagtggaact aatggtgcgt cttccgtaac atctagtgaa ggaagtcaga
     5881 gttcagaatc tgccccagcc tcacctcaac ctcaagcaca gacagctgta gctagttctt
     5941 cgagctcgtc taatgcgaat gcctcttcta gtagtgacgg gaaagctcct caggcagcaa
     6001 gcactacctc atcttcaacc ccagcagcag ccagtagtaa tggtatcagt caaggaacta
     6061 gtgctgaagc tgaaactcca atgatggagg tggaacagta tacagttggt aataaaacaa
     6121 cagaactaaa tattaaagac ggtgctcagc taaaaaacgg gagtaaggtt gataaagata
     6181 caaaacttat cagaaaccgt gatggtgagc agcgtgatat tgttgatatc aagcgtgagg
     6241 taaaaacaaa cgcagatggc actgtcgatg ttacggttac cgtaacgcct aaagaaattg
     6301 acaaaggtgc cgatgttatg gcccttctag atgtctctaa aaagatgaca gaagatgatt
     6361 ttaacaacgc taaggataag atcaagaaat tagtcacaac cttaacgagt aaatctcctg
     6421 acagtcaacc aaaccataat gctcgcaact ctgtgcgcct aatgaccttt taccgtaagg
     6481 ttaacgatcc gattgagctc aacgcagata atgtagacgc taaactagat gaggtttgga
     6541 aaaaagctaa agaagattgg gactggggcg ttgatttaca gggagctatt cacaaagctc
     6601 gagagatttt taataaagga aaaagaaaaa aatctggtaa acgccagcat atcgtcttgt
     6661 tctctcaagg tgagtcgact tttagttatg acatcaacga caaagatgaa aatgatactg
     6721 tccagaaaaa tagaatagct ggaaaagtta cgacctctaa cccactattt ccctggctac
     6781 ctatatttaa tcatacaaat caaaaagcag aagttattga tgatgtagac aaattactgg
     6841 gtttagctga aaaaatggga atatcactgc ccgatggatt aagagcagga gtgaaggcta
     6901 ttggggcagg tagtagtttc cttagtacct tcacaggatc tggattgaca gagtacctca
     6961 ctttgaatga atatcgctca gatatactaa aagaaaaaca gttcgattat accaagcgtg
     7021 taggtgaggg ctactactac cacagttatt caaagcgaac tcatggagat aagatgccct
     7081 ttgagaaaca aataagagaa gttttagaga gatttttacc gaaaatcgaa gataaagaat
     7141 ggcctaaaaa gtttatagaa atttttggat tacaagaaaa agaagtaaat caaattgggg
     7201 tagatgtaat tatgaaagta attaacagca tcttctataa gcgtgaatac cattactata
     7261 atcacaatct ttcagccatt gctgaagcca aaatggcgca gaaagagggc attacctttt
     7321 attccgttga tgttactgac tctgataagg cttccaaaag ggtaaagcga caagttggaa
     7381 atgagcagtc taaaaagaaa aaagaagatg ctgaaaaaga tcgaaacaat aagtttgacg
     7441 actatctgaa aaagatgtct gaaggtggta atttcttaag taaggttgag gagagaggta
     7501 agttcaaaga cactttaaca gagctaacga ttaaagatga atttacaaat aaggttaagg
     7561 ttcaggaaga ttcagaaggt aaaaaatata aaaccggctt aattagtgga acatcaaatg
     7621 tgacacatac ccctgctaaa accggatttt tctcatccaa tgaaagcctt acttggacca
     7681 tttccaaaga gcagttgaaa aaagcctttg aagatggaac gtctttaacc ctcacctata
     7741 agcttaaagt tgagaacgac aagtttaaaa aatctctcga ggaagataat aagaagaaga
     7801 gagcaaaacg ttctgcacct acagaaaatg aaaactctgt cacagaaaaa atcatttcaa
     7861 atactactac ctacaaaatt aatgaacaaa aagttgaagg taacaaattt gatgacgtta
     7921 atctgacata cagtaaattc aaggttcctg taccacagat tgatggccaa gttattgagc
     7981 cacaagcacc taaattacct gacttacctc ctgttattga gcatggccct gttcttgatt
     8041 acacagaaga atcaatatac cgattaccac ttgaacatgg cagcaatgca ccagacacac
     8101 aagtgacaat tgaagaagac acagttcctc aacgcccaga tatccttgta ggcggtcaga
     8161 gtggaccggt tgacat
//
LOCUS       DAUCLR010000083         7820 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.083, whole genome shotgun sequence.
ACCESSION   DAUCLR010000083 DAUCLR010000000
VERSION     DAUCLR010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7820
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.083"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..832)
                     /locus_tag="VGT87_001524"
     CDS             complement(<1..832)
                     /locus_tag="VGT87_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HEQ3814361.1"
                     /translation="MLTSKHHNLNKLVWRYGLTSAAAVLLAFGGGASSVKAEISTATM
                     TQQERAEKIKKIKDYITKYPKTTEERFWNRTWIRDYFKDEDYLKAVQSFTEQALENIL
                     ELIEKGATKGEKGDAGPAGPRGERGPQGKAGEKGETGPRGERGEQGIQGEQGKAGEKG
                     EKGDKGDTGERGEKGETGATGAQGPQGEAGKDGAPGEKGEKGDRGETGAQGPVGPQGE
                     KGETGAQGPQGEAGQPGAQGPAGPQGEAGKPGEQGPAGPQGEAGQPGEKAPEKIPEGE
                     A"
     gene            1214..1696
                     /gene="nrdI"
                     /locus_tag="VGT87_001525"
     CDS             1214..1696
                     /gene="nrdI"
                     /locus_tag="VGT87_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEQ3814362.1"
                     /translation="MPQITLVFISLSGNTLSFVKRLSLYLADNYDYHVKQINIKDLKH
                     ETFPVKEEFVAILPTYLEGGNGVDSGEVEILTTPLGEFIAAHGNAQRCLGIIGSGNKN
                     FNHQYCLTAKQYAKRFGFSLLGDFELRGTPDDISRLAQVIMEASSRHSSNDTQTLPNS
                     "
     gene            complement(2090..2908)
                     /locus_tag="VGT87_001526"
     CDS             complement(2090..2908)
                     /locus_tag="VGT87_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEQ3814363.1"
                     /translation="MTKVLTLNTHSWMQANTLKKLVALAEHILAEKYDIICLQEINQL
                     IESELATDLPRYQALSGTPSIHKDHFALLLIHYLQKRGQHYYWSWAYNHIGYDIYQEG
                     VAILSKQPIHVSDILVSAMDDETDYHTRRSLIAKTTLDGKEVAVVNVHLSWFDKGFLG
                     EWEKLEKELLTLNCPLLLMGDFNNPTDQDGYQVMMGSPLDLQDSHKVAAHVFGDHSIV
                     ADIDGWQGNKEALKVDHVFTSKDFIIRSSKITFEGGDAPVVSDHYGLEVTLDWR"
     gene            complement(2991..5177)
                     /locus_tag="VGT87_001527"
     CDS             complement(2991..5177)
                     /locus_tag="VGT87_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEQ3814364.1"
                     /translation="MKTSFKQLFRFEFWQKFGKCLMVVIAVMPAAGLMISIGNSIPMI
                     NHDSAFLASLGNIIAQIGWAVIVNLHLLFALAIGGSWAKERAGGAFASGLAFVLINRI
                     TGAFYGVSSAMLADPEAKITSLLGTQMIVKDYFTSVLESPALNTGVFVGIIAGFVGAT
                     AYNKYYNYRKLPEVLTFFNGKRFVPFVVILRSIFVALILVVVWPVIQSGINSFGMWIA
                     SSQDSAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYEVMTGAAAGTKVFGQ
                     DPLWLAWVTDLVHLKGSDASAYSHLMDSVTPARFKVGQMIGATGTLMGVALAMYRNVD
                     ADKKHTYKMMFISAAAAVFLTGVTEPLEYLFMFAAMPLYIVYALVQGASFAMADLVNL
                     RVHSFGNIELLTRTPMALKAGLGMDVINFVWVSVLFAVIMYFIADMMIKKMHLATAGR
                     LGNYDADILGDRNTQARPTQVADSNSQVVQIINLLGGAGNIDDVDACMTRLRVTVKDP
                     AKVGAEDDWKKAGAMGLIQKGNGVQAVYGPKADILKSDIQDLLDSGAVIPEVNMSQLT
                     SKPTPAKDFKHVTEDVLSVADGMVLPITGVKDQVFAAKMMGDGFAVEPTNGNIYAPVA
                     GLVTSVFPTKHAFGLLTDNGLEVLMHVGLDTVALNGVPFSVKVSEGQRVHAGDLLVVA
                     DLAAIKSAERETTIVVAFTNTAEIQDVTLTSLGNQPAKTKVATVEL"
     gene            complement(5534..6283)
                     /locus_tag="VGT87_001528"
     CDS             complement(5534..6283)
                     /locus_tag="VGT87_001528"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEQ3814365.1"
                     /translation="MQQYFIKGKAEKKVTITDKDTIKHMFQVMRLTDEAEVVLVFDDG
                     VKYLAKVTSSMAHELEIIEALPDQVELPVEVTIASGFPKGDKLDTIAQKVTELGASAL
                     WGYSADWSVVKWDGKKLAKKEDKLAKIVLGAAEQSKRNRVPEVHLFEHKEEFLKNLPS
                     FDHIFVAYEETAKAGQLATLAREVKEVKPGAKILFIFGPEGGISPTEITQFEAAGAIK
                     VGLGPRIMRAETAPLYALSALSYALELQASK"
     gene            complement(6283..7236)
                     /locus_tag="VGT87_001529"
     CDS             complement(6283..7236)
                     /locus_tag="VGT87_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEQ3814366.1"
                     /translation="METWQEVTVHVHRDAQEAVSHVLIETGSQGVAIADSADYTGQKD
                     RFGELYPDVEQSDMIAITAYYPSSTNLADVIATINEQLAELVSFGLQVGQVTVDSQEL
                     AEEDWADNWKKYYEPARITHDLTIVPSWTDYDASAGEKVIKLDPGMAFGTGTHPTTKM
                     SLFALEQVLRGGETVIDVGTGSGVLSIASSLLGAKTIYAYDLDDVAVRVAQDNIDLNQ
                     GTDNIHVAAGDLLKGVSQEADVIVANILADILVLLTDDAYRLVKKEGYLILSGIISEK
                     LDMVLEAAFSAGFFLETHMIQGEWNALVFKKTDDISGVIGG"
     gene            complement(7253..>7820)
                     /locus_tag="VGT87_001530"
     CDS             complement(7253..>7820)
                     /locus_tag="VGT87_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814367.1"
                     /translation="MLLGALKQFLDNLSDIKSFDFWLSYAFELLVILINLPVLYIAQK
                     MIIIEKIIVHSEYPNTIVSFMRYYYKWYCRKIKFKKLIVKDYNLDIAVQKYIFGYPKI
                     SVYVKEGNLSKEKVLNLIALIIVKGDKKEKLSRRIDRFPVYIEVVDKENQTVALWTEE
                     FLSKQNYFYDPFMTKNTKEIYPSILMLQ"
BASE COUNT         2082 a         1849 c         1514 g         2375 t
ORIGIN      
        1 ctgcttcgcc ttctgggatc ttttctggag ctttttcgcc tggttggcct gcttcacctt
       61 gtgggcctgc tgggccttgc tcacctggtt tgcctgcctc accttgtggg cctgctgggc
      121 cttgtgcgcc tggttggcct gcttcgcctt gtgggccttg tgcgcccgtt tcaccttttt
      181 caccttgtgg gcctactgga ccttgagctc cggtttcacc gcggtcacct ttttcaccct
      241 tttcgcctgg agctccatct ttaccagctt caccttgtgg gccttgtgcg ccagttgctc
      301 cggtttcacc tttttcaccg cgctcaccgg tatcaccttt gtcgcctttt tcacccttct
      361 cgccagcttt accttgctcg ccttggattc cttgttcacc gcgctcacct ctcggaccgg
      421 tttcaccttt ttcaccagct ttaccttgtg ggccgcgttc gcctcgaggt cctgctgggc
      481 cagcatcccc tttttcacct tttgttgcac ctttttcaat cagttctaaa atattttcaa
      541 gtgcttgttc agtaaaactt tgtactgctt ttaaataatc ttcatcctta aaatagtcac
      601 ggatccaggt ccgattccaa aatctttctt cagtggtttt tggatatttg gtaatatagt
      661 ctttaatttt tttaattttt tcagctctct cctgctgtgt catagtcgca gtagaaatct
      721 cagccttaac gctgcttgcc ccgcctccaa aggctagaag gacggcagcg gctgaggtta
      781 gcccgtagcg ccagaccagt ttgttgagat tatggtgctt tgatgtcaac atatgttgtt
      841 ctctctttct ttattagatg gtgtgtttta gataaaaata acatggtagc tagacctgat
      901 tatttataat agtattataa tagagtgtga aaacgattgt aaagttctct tttgttgttc
      961 atcttttaga ttttgactga tcgctctttt gtggagatca gagggccact ttagcacttg
     1021 cctgcctcgt ttttaagttg tgggcatccg gatcctttct ttaaggtcac tcttttttga
     1081 aatgttactt tttttgactg cttgtacctt cttgtctggt tggataccgc taggtttctt
     1141 ctttacttgt ttgttggtct tgattgtggt aagcttatga agaaaccttg taggataaaa
     1201 aaaggagaca cttatgcctc agattaccct tgtttttatt agcctcagtg gcaacaccct
     1261 tagctttgtc aagcggctat ccctttatct agcagacaat tacgattacc atgtcaaaca
     1321 gattaacatt aaagatttga agcacgagac ttttcccgtc aaagaagagt ttgtggcgat
     1381 tctacccact tatctagaag gcggaaatgg cgttgactct ggtgaggtgg agatcctcac
     1441 cactcctttg ggagagttta ttgctgccca tggcaacgcc cagcgttgtc ttgggattat
     1501 tggttcaggc aacaaaaact ttaaccatca gtattgcctg acagccaaac aatatgccaa
     1561 acgctttggc ttttcactgc taggtgattt tgagctgcga gggactcctg atgacatttc
     1621 ccggttagcc caggtcatca tggaggctag ctccagacac tcatctaacg acacacagac
     1681 actgcccaac agctagacag tctatagatg atcatagctg ttttaaaaaa gagcgctttt
     1741 aggacaataa ctacagaggt ctcttacccc acgccttgtg atttctcagc ctttgaaaag
     1801 agcgatcaca gtgcttcttg gtgcttggga tcacaccaag cactcctaga aataccccca
     1861 tgcagacttt agcaaggcaa ctgaacgctt ggcggtactt tctgtgaagc tcagatggtg
     1921 ggtgacaaaa aagccctggt tttttagggt tactgacatg actaaagagg tctaaccagc
     1981 tctaccttga gctagttaga cctctttgtg ttctcctaat ccccctagat gatcgcagtt
     2041 tcttatgtgt cttggcttct cctatgtggt gtgcctgtta gcacatgctc taacgccagt
     2101 ctaatgtcac ttctagaccg tagtggtcac tgacgactgg cgcatctccg ccttcaaagg
     2161 taatcttaga agatcggatg atgaagtcct tactggtaaa gacatgatct accttgaggg
     2221 cttctttgtt accttgccag ccatcaatat cagccacgat gctatgatca ccaaacacgt
     2281 gtgcagctac tttgtgacta tcttgtaggt ctagggggct acccatcatc acttgataac
     2341 cgtcttgatc cgttggattg ttaaaatctc ccataagtag caagggacag tttagtgtga
     2401 gcagctcttt ttctaatttc tcccactccc ctaaaaaccc tttgtcaaac caagataggt
     2461 gcacattgac aacagcgact tctttgccat ctaacgtggt tttggctatg agtgagcgtc
     2521 tggtgtggta gtctgtttca tcatccatcg ctgacacgag aatatctgaa acatgaatag
     2581 gttgttttga taagattgcc acgccttcct ggtaaatgtc ataaccaata tggttataag
     2641 cccaagacca ataatagtgc tgccctcgtt tttgtaaata atggatcaag agcagagcaa
     2701 aatggtcttt atgaatgctt ggtgtccctg aaagagcttg atatcttggt aaatctgtgg
     2761 ccagctcgct ctcgattaac tgattgattt cttgcaagca aatgatatcg tatttttcag
     2821 ctaaaatatg ctcagccaaa gcaactagtt ttttaagagt gttggcttgc atccacgaat
     2881 gggtattcag tgttaatacc ttggtcatta atcctccttt ataagacaag aagagccata
     2941 gggcctaaac cagctatggc tattacctat cactatctcg tgatgttttg ttaaagttct
     3001 acggtggcta ctttagtctt agcaggttga ttgcctaaag aagtcaaggt cacatcttgg
     3061 atctctgctg tgttggtaaa agcaacaaca atggttgttt cacgctctgc tgatttaata
     3121 gcagcaagat ctgctacgac taataggtca cctgcatgaa cccgttgccc ttctgagact
     3181 ttgactgaaa aaggcacacc atttaaggcg acggtatcaa gtcccacgtg catcagcact
     3241 tcaagaccat tatctgtcag taaaccaaag gcatgcttgg tcggaaagac actggtcaca
     3301 aggccagcta cgggtgcata gatattacca tttgttggtt caaccgcaaa cccatctccc
     3361 atcatcttag ccgcaaacac ctggtctttc acacctgtga ttgggagaac catcccgtct
     3421 gccactgata gcacatcttc tgtcacgtgt ttaaaatctt ttgcgggagt gggtttgcta
     3481 gtgagctgtg acatattgac ttctggaata acagcccctg agtccagcaa atcttgaata
     3541 tctgatttca aaatatctgc tttagggcca tagaccgctt gaacgccgtt gcctttttgg
     3601 atcaagccca tagcgccagc ttttttccaa tcgtcctcag cgccaacttt agcggggtct
     3661 ttgaccgtca ctcgtaagcg cgtcatacaa gcatcaacat catcaatatt gcctgcccca
     3721 ccaaggagat taatgatttg tacgacttga gagttgctat ctgctacttg ggtaggtctt
     3781 gcttgagtgt tacggtcacc cagtatatcg gcatcgtagt tgcctaaacg gccagctgtt
     3841 gcgagatgca ttttcttgat catcatatct gcaataaagt acatgataac tgcaaagagg
     3901 acagaaaccc aaacaaagtt aatcacatcc atgcctaggc ctgctttaag agccataggg
     3961 gtacgggtta gtagctcaat gttcccaaat gagtgaacac gaaggttcac aaggtcagcc
     4021 atagcaaatg aagctccttg aacaagagca tagacgatgt aaagtggcat tgctgcaaac
     4081 ataaataggt actcaagtgg ctcagttacc cctgtcaaaa agaccgctgc cgctgctgag
     4141 atgaacatca ttttgtatgt gtgtttttta tcggcatcta cattgcggta catggctaag
     4201 gcaaccccca tcaatgttcc ggtagctcca atcatttgtc ctaccttaaa gcgagctgga
     4261 gtcacactat ccattaagtg actataggct gaagcgtctg atcctttaag gtgaacaagg
     4321 tcagttaccc aagcaagcca caaagggtct tgaccaaata cttttgtccc cgctgctgca
     4381 cctgtcatga cctcataagt tccaccaaga gctgtatagt tcattgggat cgtcaacatg
     4441 tggtgaagac caaatggcaa caagagacgt tccaaggtac catataggaa tggggctagg
     4501 attggagctg aatcttgtga agaggcaatc cacatcccaa aactgttaat cccagactgg
     4561 atcaccggcc aaacaacaac caaaataagg gctacaaaaa tagaacgtaa aataacgaca
     4621 aacggtacaa aacgcttccc attaaagaag gtcaaaactt caggaagttt acggtaatta
     4681 tagtatttgt tataggccgt tgctcctacg aaccctgcaa taatcccaac aaaaacccct
     4741 gtgtttaaag ctggagattc caagacactg gtgaaataat ctttaacgat catttgagtg
     4801 ccaaggaggc ttgtgatttt tgcttctgga tcagctagca tagcactaga cacgccataa
     4861 aaagcacctg ttatccgatt gatcaagaca aaagcaagac ctgaggcaaa ggcccctcca
     4921 gctctttctt tagcccaact accaccaatg gccaaggcaa atagcaaatg aaggtttaca
     4981 ataacagccc agccaatctg agcgataatg ttgcctagag atgccaaaaa tgctgagtcg
     5041 tggttaatca ttggaataga gtttccgata ctgatcatta aaccagccgc tggcatgaca
     5101 gcaataacga ccataagaca tttcccaaat ttttgccaaa attcgaaacg aaacagttgt
     5161 ttgaatgatg ttttcattct atctctcctt tattatcccc ttttagccat agctgccctt
     5221 aaggcacttt tgtgacacaa tgatatagac tacaactcag taatgcaatc gtttgcattg
     5281 atctgatacc tttattatac tagcctcgca acaatttgta aagcgttttc ttagacagat
     5341 gttagtcgtt tttcttcatt aaatggtagt gtaactcacc aaaaatatga caagagccca
     5401 tttccttttt tggtgatgag cccatcaacg ctcttcaaaa aaacaagaag caataaaaaa
     5461 aggccgttga cacaatcgtg aactgcctgt tcgtttcttt agcctcttct ttctaaagta
     5521 agcagcagct tacttatttt gatgcctgca attccaaagc ataactaaga gcacttaagg
     5581 catatagcgg cgctgtctct gctcgcataa tgcgagggcc taagcccacc tttatggcac
     5641 ctgctgcctc aaactgagtg atttcagttg gggaaattcc cccttctggc ccaaagataa
     5701 agaggatttt tgcccctggc ttaacttctt ttacttcacg cgccaaggtc gctaactgac
     5761 ctgccttggc tgtttcttcg tatgcaacga aaatatgatc aaagctagga aggtttttta
     5821 gaaactcttc tttgtgttca aagaggtgaa cttcaggcac cctatttcgt ttgctctgct
     5881 cagccgctcc taatacaatc ttagccaatt tgtcttcttt tttagctaat tttttgccat
     5941 cccatttgac cactgaccag tctgcggagt agccccatag cgcactggct cccaattcag
     6001 tcactttctg ggcaatcgtg tctaacttat cgcctttagg aaatccagaa gcaatggtca
     6061 cctcaacagg caattccacc tgatctggca aagcctctat aatctctaac tcatgagcca
     6121 tactactggt cactttggct aaatacttca ccccatcatc aaataccaag acaacttctg
     6181 cctcgtcagt caagcgcatc acttgaaaca tgtgcttgat ggtatcttta tcggtaatcg
     6241 ttaccttttt ttctgctttg cctttaataa aatactgttg cattagccgc caatcactcc
     6301 tgagatatca tctgtttttt taaagacaag ggcgttccac tcgccttgaa tcatgtgtgt
     6361 ctcaaggaag aagcctgcgc taaaggcagc ttccagaacc atatccaatt tctcagaaat
     6421 gatgcctgat aagataaggt aaccttcctt tttgaccaaa cggtaagcat catcagtcaa
     6481 taagactaag atatctgcca aaatattagc cacaatcaca tccgcttctt ggctaacccc
     6541 ttttaaaagg tcaccagcag ccacatgaat gttgtctgtt ccttggttta aatcaatgtt
     6601 gtcttgagcc actcgaacag ccacgtcatc taagtcataa gcgtagatgg ttttagcacc
     6661 tagtaaggaa ctggcaatcg agaggacacc tgatccggtc cctacatcga taactgtctc
     6721 accaccacga aggacttgtt ccaaggcaaa aaggctcatc ttagttgtgg gatgtgtccc
     6781 tgtcccaaat gccattccag gatctaactt aataaccttt tccccagcag aagcgtcata
     6841 atctgtccaa ctagggacaa tggttaaatc atgcgtgata cgagctggtt catagtattt
     6901 tttccaattg tctgcccagt cttcctcagc caattcctga ctgtccacag taacctgacc
     6961 aacctgtaac ccaaaactaa ctaactccgc taactgttca ttaatggtcg caataacatc
     7021 cgccaaatta gtgctacttg ggtaataggc tgtgatagct atcatatcag actgctccac
     7081 atctgggtat aactccccaa agcggtcctt ttgcccagta tagtctgcgc tatccgcgat
     7141 agcgacccct tgactgcctg tctcaatcaa cacatgtgaa acagcctctt gcgcatcacg
     7201 atgcacatga accgtcactt cttgccacgt ttccataagt atgtcctttc tcctactgca
     7261 acattaaaat cgatggataa atttcctttg tatttttagt cataaatgga tcataaaaat
     7321 aattttgttt agataagaac tcctcagtcc ataatgctac tgtttgattc tctttatcaa
     7381 ccacttcaat ataaacagga aatctatcta tccttcgaga taatttttcc ttcttatctc
     7441 cttttacaat tattaacgct attaaattca gaaccttctc tttagagagg ttaccctctt
     7501 taacgtatac agaaatttta gggtatccaa aaatatattt ttgtactgca atatctaaat
     7561 tataatcttt tactattaat tttttaaatt ttatttttct acaataccat ttataatagt
     7621 atctcataaa actaacaata gtatttggat actcactatg aacaattatt ttttcaataa
     7681 ttatcatttt ctgtgctata tacaatactg gaagattaat cagtatgaca agtaattcaa
     7741 aagcatatga taaccaaaaa tcaaatgatt taatatcgga aagattgtcc aaaaattgtt
     7801 ttaacgcacc aagtagcatt
//
LOCUS       DAUCLR010000084         7706 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.085, whole genome shotgun sequence.
ACCESSION   DAUCLR010000084 DAUCLR010000000
VERSION     DAUCLR010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7706
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.085"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            237..2237
                     /gene="metG"
                     /locus_tag="VGT87_001531"
     CDS             237..2237
                     /gene="metG"
                     /locus_tag="VGT87_001531"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEQ3814368.1"
                     /translation="MMKKPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGHEV
                     FYLTGLDEHGQKIQTKAKEAGITPQTYVDNMAKDVKALWQLLDISYDTFIRTTDDYHE
                     EVVAAVFEKLLAQDDIYLGEYSGWYSVSDEEFFTESQLKEVFRDEDGQVIGGIAPSGH
                     EVEWVSEESYFLRLSKYADRLVAFFKERPDFIQPDGRMNEMVKNFIEPGLEDLAVSRT
                     TFTWGVPVPSDPKHVVYVWIDALLNYATALGYGQANHANFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDLPMPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERFGLDPLRY
                     YLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVAMINKYFDGAVPAYVDNGT
                     AFDADLSQLIDAQLADYHKHMEAVDYPRALEAVWTIIARTNKYIDETTPWVLAKEDGD
                     KAQLASVMAHLAASLRVVAHVIQPFMMETSAAIMAQLGLEPVSDLSVLALADFPANTK
                     VVAKGTPIFPRLDMEAEIDYIKAQMGDSSAISQEKEWVPEEVALKSEKDVITFETFDA
                     VEIRVAEVKEVSKVEGSEKLLRFRVDAGDGQDRQILSGIAKSYPNEQELVGKKLQIVA
                     NLKPRKMMKKYISQGMILSAEHGDQLMVLTVDSSVPNGSIIG"
     gene            2732..3745
                     /gene="nrdF"
                     /locus_tag="VGT87_001532"
     CDS             2732..3745
                     /gene="nrdF"
                     /locus_tag="VGT87_001532"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEQ3814369.1"
                     /translation="MTQHYYERSQSPIEYALSETQKQLRSINWNYLNDDKDLEVWNRV
                     TQNFWLPEKVPVSNDLNSWRSLGEDWQQLITRTYTGLTLLDTVQATVGDVAQIQHSQT
                     DHEQVIYTNFAFMVGIHARSYGTIFSTLCSSEQIEEAHEWVVSTQSLQDRARVLIPYY
                     TGDDPLKSKVAAAMMPGFLLYGGFYLPFYLSARGKMPNTSDIIRLILRDKVIHNYYSG
                     YKYQQKVARLSPEKQAEMKAFVFDLLYELIDLEKAYLRELYAGFDLAEDAIRFSLYNA
                     GKFLQNLGYESPFTDEETRVSPEVFAQLSARADENHDFFSGNGSSYVMGITEETTDDD
                     WEF"
     gene            3749..4237
                     /gene="nrdI"
                     /locus_tag="VGT87_001533"
     CDS             3749..4237
                     /gene="nrdI"
                     /locus_tag="VGT87_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEQ3814370.1"
                     /translation="MAELIIVYFSSKSNNTHRFVQKLGLPAQRIPVDNRPLEVSTHYL
                     LIVPTYAAGGSDAKGAVPKQVIRFLNNPNNRKHCKGVISSGNTNFGDTFALAGPIISQ
                     KLQVPLLHQFELLGTATDVKKVQAIFARLKHHTHDKQKQINNLITERTHPCHKPMRHT
                     SH"
     gene            4204..6384
                     /gene="nrdE"
                     /locus_tag="VGT87_001534"
     CDS             4204..6384
                     /gene="nrdE"
                     /locus_tag="VGT87_001534"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEQ3814371.1"
                     /translation="MSQTNASYLSLNALTRFKKPDGSYHFDSDKEAVRRYLEEHVSPN
                     QMAFNSLEDKLAYLINEGYYEQAIFDAYPNDLIKEAFHYAYQQGYRFLNLMGAMKFYQ
                     SYALKTLDGKQYLETFEDRAVMNALFLADGDQAFVFDVIDAILHRRFQPATPTFLNAG
                     KKRRGEYISCYLLRVEDNMESISRAISTSLQLSKRGGGVALCLTNLREIGAPIKGIEN
                     QATGIVPVMKLLEDSFSYANQLGQRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIK
                     SLALGLVIPDITFQLAKENKDMALFSPYDIKRAYGKDMSDISITEEYDKLLANPAIKK
                     TYISARKLFQLIAELHFESGYPYLLFDDTVNKRNPHAKKGRIVMSNLCSEIAQVSTPS
                     TFKEDLSFETIGEDICCNLGSINIAQAMADAPHFEQLITTSIRALDRVSRVSDLNCAP
                     SVETGNAANHAVGLGAMNLHGFLATNHIYYDTKEAVDFTDLFFHAMAYYAFKASCQLA
                     KEKGAFAGFSLSTYADGTYFAKYLQEDAKPQTAKVATLLQDYGFTLPTVADWQALVAD
                     IKQFGLANAHLLAVAPTGSISYLSSCTPSLQPVVAPVEVRKEGSLGRIYVPAYQIDQA
                     NYAYYERGAYEVGPKAIIDVVAAAQKHVDQAISLTLFMTDQMTTRDLNRSYIQAFKQN
                     CASIYYVRVRQDVLAGSEQYDEDSLVTAPGASDETTTECQSCMI"
     gene            complement(6545..7297)
                     /locus_tag="VGT87_001535"
     CDS             complement(6545..7297)
                     /locus_tag="VGT87_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosyltransferase"
                     /protein_id="HEQ3814372.1"
                     /translation="MLKKRYQLAIVLLLSCFSLIWQTGGLVELFVCEHYERAVCEGTP
                     AYFTFSDQKGAATLIKKRWGKGLVYPRAEQEAMAAYTCQQAGPINTSLDKAKGELSQL
                     TPELRDQVAQLDAATHRLVIPWNIVVYRYVYETFLRDIGVSHADLTSYYRNHQFDPHI
                     LCKIKLGTRYTKHSFMSTTALKNGAMTHRPVEVRICVKKGAKAAFVEPYSAVPSEVEL
                     LFPRGCQLEVVGAYVSQDQKKLHIEAYFKGSL"
BASE COUNT         2218 a         1757 c         1647 g         2084 t
ORIGIN      
        1 gggcacaaaa ggatgaagag aaagttttca tgcgtacggc gtgccctcct ttgattgact
       61 ggttttgctg ccccttaaaa tatgatataa tagactattg atagctgtta cttaacagaa
      121 ggctgataaa aaaggttcca ttagccttga gggttgaatt taaacaggaa aaaacgaaat
      181 cctacactat gcatctcaat catactttca tttgagtatg gttctgaaag gaaactatga
      241 tgaaaaaacc attttacatt acgacaccca tttattaccc atctgggaaa ttacacattg
      301 gttctgccta tacaactatt gcttgtgatg tcttggcacg ctacaagcgc ttgatgggcc
      361 atgaggtctt ttacctcaca ggacttgatg agcacggaca aaagattcaa accaaggcaa
      421 aagaagctgg tattacacct caaacctatg ttgataacat ggcaaaggat gtcaaagccc
      481 tttggcaatt gctagatatt tcctatgaca catttatccg gacaacggac gattaccacg
      541 aagaggtcgt tgctgctgtc tttgaaaaat tgctggcgca agacgacatt taccttggcg
      601 aatactctgg ttggtattct gtctctgatg aggaattttt cactgaaagc caattaaaag
      661 aagtctttag agatgaagac ggtcaggtta ttggaggaat tgccccatca ggtcatgagg
      721 tcgagtgggt ctctgaagaa tcctatttcc tacgccttag caaatacgct gatcgtcttg
      781 tggccttctt taaagagcga cctgacttta tccaaccaga cggtcgtatg aatgaaatgg
      841 tgaaaaactt tatcgagcca ggtctagaag atttggctgt tagccgtact acctttacat
      901 ggggtgtacc tgttccatca gacccaaaac acgtggttta tgtctggatt gatgccctcc
      961 taaactatgc gacagctctt ggttatggtc aagctaatca cgctaacttt gataaattct
     1021 ggaatggaac ggtttttcac atggttggaa aagatatctt gcgtttccac agtatctact
     1081 ggccaatctt actcatgatg cttgatttac caatgccaga ccgcttaatt gctcatggtt
     1141 ggtttgtcat gaaagacggt aaaatgtcta aatcaaaagg caatgtggtc tatccagaaa
     1201 tgttagtaga gcgctttgga ctagatcctc ttcgttatta cctcatgcgt tctttgccag
     1261 tgggttcaga cgggaccttt acccctgaag attatgtggg tcgtatcaac tatgagttag
     1321 ccaacgacct tggcaattta ttgaaccgta ccgttgcgat gatcaacaag tattttgacg
     1381 gcgcggtgcc agcttatgtt gacaatggaa ctgcctttga tgctgactta agtcaactga
     1441 ttgatgctca gctagcagac taccataagc acatggaagc tgtagactac ccacgcgcct
     1501 tggaggctgt ttggactatc attgctcgta ccaacaaata catcgatgaa accactcctt
     1561 gggtgctcgc taaagaagat ggtgacaaag ctcaactagc cagtgtcatg gcacacttag
     1621 cagctagctt gcgcgtggtc gctcatgtga tccaaccctt tatgatggaa acatcagctg
     1681 ctatcatggc ccagcttggt ttggaacctg tttctgattt gtcagttctt gctttagcag
     1741 attttcctgc caatacaaag gtcgttgcca aaggcacacc gattttccca cgtcttgata
     1801 tggaagctga gattgactac atcaaagccc aaatgggtga cagctcagct atttcccaag
     1861 aaaaagaatg ggtccctgaa gaggttgcgc tcaaatctga aaaagacgtc atcacatttg
     1921 aaacctttga tgctgtggaa atccgtgtgg cagaagtcaa ggaagtgtca aaagtggaag
     1981 gttcagaaaa attgctgcgc tttagagtag atgcaggaga cgggcaagac cgtcaaatct
     2041 tgtcaggtat tgccaaatcc taccccaacg aacaagaatt agtgggtaaa aaactacaaa
     2101 ttgttgctaa cctgaaaccg cgtaaaatga tgaaaaaata catcagccaa ggcatgattt
     2161 tatcagcaga gcatggagat cagctaatgg tcttaaccgt tgattcctct gttccaaatg
     2221 gaagtatcat tggctgatgc tgacttatta ttcaaaaaac ctaagcaaga acatgtgctt
     2281 aggttttttt gattgccttc aagaaagacg aataacgaga tgggtgatat ggcagcaaaa
     2341 gcataactat cctaggacct gtcctagtaa gattgctgtg cttttgacaa gtgaacagag
     2401 aggtcttaaa actatatctt gtcataaaaa agattgcaaa acactatatc ttgtgttatt
     2461 atggttttta taaaagttaa gcacgcttaa ctttagccaa acaaaagagg acactagagc
     2521 gtgtcccaca agacagttga gcccttcgct tttttagtag agtaggagtg ctcaagtcaa
     2581 aaagagacat cccatcaaac cattgctgtt tagcaggtct ttttgtgttg ctcaaaaaaa
     2641 caattcctgt ctgtttggcg tctagtgatg acttaggctt agcgctacta gacttttgtt
     2701 tgaaaaatac ctcaagcctt gaaggagaca aatgacccaa cattattatg aacgttcgca
     2761 atccccaatt gaatacgccc tatctgaaac acaaaaacag ttgcgctcca tcaattggaa
     2821 ttaccttaat gatgacaaag atttggaagt gtggaaccgt gtcactcaaa atttttggtt
     2881 gcctgaaaag gttcctgttt ctaatgattt aaattcttgg cgctccttgg gtgaggattg
     2941 gcaacagctc atcacaagga cctacactgg gttgacctta ctagataccg tacaagcaac
     3001 ggttggtgat gtggcacaaa ttcagcattc acaaaccgac cacgaacaag tcatctatac
     3061 gaactttgcc tttatggtgg ggattcatgc ccgctcttac gggacgattt tttcgaccct
     3121 ttgcagcagc gagcaaattg aagaagcaca tgaatgggtg gtctcaacac agagcttgca
     3181 agaccgtgcc cgtgtcttga ttccttacta tacgggtgat gaccctttga aatcaaaagt
     3241 agcagcagct atgatgccag gatttttgct gtatggcggc ttttaccttc ctttttacct
     3301 atcagcacgt ggtaaaatgc caaacacatc tgatatcatc cgcctgattt tacgcgacaa
     3361 agttattcac aactattaca gcggttacaa ataccaacaa aaagtagcac gtcttagccc
     3421 agaaaaacag gctgaaatga aggcctttgt ctttgacttg ctctatgaac tcatcgacct
     3481 tgaaaaagct tacttgagag aactgtatgc aggctttgat ttggcagaag atgctatccg
     3541 ctttagcctt tataacgctg gaaaattcct tcaaaatttg ggatatgagt cgcctttcac
     3601 agatgaagaa acgcgcgttt caccagaagt ttttgctcag ctatcagcgc gtgcagacga
     3661 aaaccatgac tttttctcag gcaatggctc atcttatgtg atggggatca ccgaagaaac
     3721 cacagatgac gattgggagt tttaagaaat ggcagagctg attattgtgt atttttcatc
     3781 taagtccaat aacacccatc gcttcgtcca aaaattaggc ctgcctgctc agcgcatacc
     3841 agtagataat aggccacttg aagtttcaac ccattacctt ttgattgtgc ctacctatgc
     3901 agctggtggc tcagatgcca aaggagccgt ccctaaacaa gtgattcgtt ttttgaataa
     3961 tcctaacaat cgtaaacatt gtaagggagt gatctcttca ggaaatacca attttggcga
     4021 tacctttgcc ttagcaggcc ctatcatttc tcaaaaattg caggtcccct tattacacca
     4081 atttgaacta ttaggcactg ctacagatgt caaaaaggta caggcgattt ttgcccgctt
     4141 aaagcatcac acacacgaca agcaaaaaca aatcaacaac ctgattacag aaaggacaca
     4201 cccatgtcac aaaccaatgc gtcatacctc tcattaaatg cactgacccg ttttaaaaaa
     4261 ccagacggca gctaccattt tgatagcgat aaagaagctg ttaggcgcta cctggaagaa
     4321 catgtctctc ccaatcaaat ggcttttaac tcactagaag ataagctagc ttacctcatc
     4381 aatgaagggt attatgagca ggccattttt gatgcctacc ctaacgacct tatcaaggag
     4441 gcctttcatt atgcctatca acaaggctat cgctttttaa atctcatggg cgctatgaaa
     4501 ttttaccagt cctatgcctt aaaaaccttg gacggtaagc agtatctaga aacatttgaa
     4561 gaccgtgctg tgatgaatgc actcttttta gctgatggag atcaagcttt tgtctttgac
     4621 gtgattgatg ccatcttgca ccgacgcttt caaccagcta ccccaacgtt tttaaatgcc
     4681 ggaaaaaaac gccgtggcga atacatttcg tgctatcttc ttcgtgtcga agacaatatg
     4741 gaatctatct ctcgcgccat cagcaccagc ttgcaactct caaaaagagg aggcggagtc
     4801 gccttgtgtc tcaccaacct cagagaaatc ggagcaccga taaaagggat tgaaaatcag
     4861 gcaacaggca tcgttcctgt catgaaacta ctagaggact ctttttccta tgccaatcaa
     4921 ctaggtcagc gccaaggagc aggtgcggtt taccttcatg cccatcatcc tgaggtcttg
     4981 acctttttag acaccaaacg tgaaaatgcc gatgagaaaa ttcgcattaa gtccctagca
     5041 ttgggacttg tgatcccaga tattaccttc cagttagcca aagaaaacaa agacatggct
     5101 ctcttttcac cctatgatat caagcgtgcc tatggcaagg acatgtcaga catctccatc
     5161 acagaggaat acgataaact gctagccaat ccagccatca aaaagaccta catcagtgct
     5221 agaaagctct ttcagttaat tgcagagcta cattttgagt caggctaccc ttacctcttg
     5281 tttgatgaca cggtcaataa gcgcaaccct catgccaaaa aaggcagaat cgtcatgtct
     5341 aacctgtgtt ctgaaatcgc tcaagtcagc accccaagca ctttcaaaga agatttgagt
     5401 tttgaaacca ttggagagga tatctgctgt aatttagggt ctatcaatat tgcccaggct
     5461 atggcagatg cgccacactt tgaacagctc atcacaacga gcatcagagc acttgatcgt
     5521 gtctctagag tgtctgacct gaattgtgcg ccttctgttg agacaggcaa tgcagccaac
     5581 catgcagtag gattaggcgc catgaaccta catgggtttt tggcaaccaa ccacatctac
     5641 tatgatacaa aagaagccgt tgactttaca gatctgtttt tccatgccat ggcctattat
     5701 gcttttaaag cctcttgcca attagccaaa gaaaaaggcg cttttgcagg attttcgtta
     5761 tcaacctatg cagatggcac ttattttgcc aagtatttgc aagaagatgc caagccacaa
     5821 acagccaagg ttgccacatt gttgcaagat tacggcttca cattgccaac ggtggcggac
     5881 tggcaagccc tagtagcaga tatcaagcag tttggtctag ccaatgcgca cttgctagcg
     5941 gttgctccga caggctccat cagctactta tcatcctgca ctccgagtct acaaccagtc
     6001 gttgctcctg ttgaggtccg aaaagaaggt agcctgggca ggatttacgt ccctgcctat
     6061 cagattgacc aggctaacta tgcttactat gaacgtggtg cctatgaggt cggcccaaaa
     6121 gccatcatcg acgttgtcgc tgctgcccaa aagcacgttg accaagccat ctcattgacc
     6181 ctgtttatga ctgatcaaat gaccacacgt gaccttaacc gctcctatat ccaagctttt
     6241 aagcaaaact gtgcatcgat ctattatgtg cgagtgagac aagacgtcct agcaggcagc
     6301 gaacagtatg acgaggatag tcttgtgaca gctcctggag ctagtgatga gaccaccaca
     6361 gagtgccaaa gctgtatgat ctaacagcta ctagaaagct ctcgtgtccc ctatcacatg
     6421 cataggatca gtgcgctcga ccattcaaga caagcaagca tcagctcttg cttgtctttt
     6481 tttgagcatc agaacgtcaa aaaaaggaca tggagtcctt ttttggtgat cggtgttgag
     6541 gccgtcacaa actgcccttg aaatacgctt ctatgtggag ctttttttgg tcctgtgaca
     6601 cgtaagctcc aacgacctcc agctgacagc ctcttggaaa caagagctca acctctgaag
     6661 gcacagccga ataaggctcg acaaaggctg ccttggcccc ttttttgaca cagatgcgca
     6721 cctccaccgg tcgatgggtc atggcgccat ttttcaaggc tgtcgtgctc ataaaactgt
     6781 gcttggtgta gcgtgtgcca agcttgatct tacaaaggat atgagggtca aactgatggt
     6841 tacggtagta agacgtgaga tcagcgtgtg aaacaccgat atcacgcaaa aacgtctcgt
     6901 atacatagcg gtatactaca atgttccacg ggatgactag ccggtgagtc gcagcatcga
     6961 gctgggccac ctgatccctt agctcaggcg tgagttggct gagctcacct ttggccttgt
     7021 ctaggctggt gttgataggg cctgcctgct ggcaggtata agcagccatc gcctcttgct
     7081 cagcccttgg gtagacgaga cccttgcccc atcgcttttt aatcagtgtc gcagctccct
     7141 tttgatccga aaaggtaaaa taagcaggcg tcccctcaca aaccgcccgc tcatagtgct
     7201 cacagacaaa aagctcgacc aagcccccag tttgccagat cagactaaaa cagctaagaa
     7261 gaaggacaat agcgagttga tagcgctttt ttagcatagt tccccctgac accagcccaa
     7321 aaggctgacc cttcccctag accttttggc tagctcttga caccaccagt ttctcagaca
     7381 ggctaatctt tttgtcatct ctttctccct aaacagactc tcttatcatg atattatgct
     7441 attatgagcg atttgtcaat ccatagtaac cgttttttag ttagataagt aagcttttga
     7501 cctagaggca gacaagagtg gcagtacctg ccgtttgctg tggtggctaa taaggactaa
     7561 cataaaatct caaacgcttt caaaaatagc tcctgagctt aaaaaaggcg aattaccaga
     7621 tttgttgagc ttaagggaga ttgccatgat tttctaatca tttgatggtt aaaatgataa
     7681 aaacatgaac ttgaagtgaa agaatg
//
LOCUS       DAUCLR010000085         7563 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.086, whole genome shotgun sequence.
ACCESSION   DAUCLR010000085 DAUCLR010000000
VERSION     DAUCLR010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7563
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.086"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..427
                     /locus_tag="VGT87_001536"
     CDS             <1..427
                     /locus_tag="VGT87_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEQ3814373.1"
                     /translation="QKAVADTTSVATSNGLSYAPNHAYNPMNAGLQPQTAAFKEEVAS
                     AFGITSFSGYRPGDPGDHGKGLAIDFMVPENSALGDQVAQYAIDHMAERGISYVIWKQ
                     RFYAPFASIYGPAYTWNPMPDRGSITENHYDHVHVSFNA"
     gene            743..2515
                     /locus_tag="VGT87_001537"
     CDS             743..2515
                     /locus_tag="VGT87_001537"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEQ3814374.1"
                     /translation="MYYTSGNYEAFATPRKPEGVDQKSAYIVGTGLAGLAAAVFLIRD
                     GHMAGERIHLFEELPLAGGSLDGIEKPHLGFVTRGGREMENHFECMWDMYRSIPSLEI
                     PGASYLDEFYWLDKDDPNSSNCRLIHKRGNRVDDDGQYTLGKQSKELIHLIMKTEESL
                     GDQTIEEFFSEDFFKSNFWIYWATMFAFEKWHSAVEMRRYAMRFIHHIDGLPDFTSLK
                     FNKYNQYDSMVKPIIAYLESHDVDIQFDTKVTDIQVEQTAGKKVAKTIHMTVSGEAKA
                     IELTPDDLVFVTNGSITESSTYGSHHEVAKPTKALGGSWNLWENLAAQSDDFGHPKVF
                     YQDLPAESWFVSATATIKHPAIEPYIERLTHRDLHDGKVNTGGIITITDSNWMMSFAI
                     HRQPHFKEQKDNETTVWIYGLYSNSEGNYVHKKIEECTGQEITEEWLYHLGVPVDKIK
                     DLASQDYINTVPVYMPYITSYFMPRVKGDRPKVIPDGSVNLAFIGNFAESPSRDTVFT
                     TEYSIRTAMEAVYSFLNVERGIPEVFNSAYDIRELLKAFYYLNDKKAIKDMDLPIPAL
                     IEKIGHKKIKDTFIEELLKDANLM"
     gene            2674..3726
                     /locus_tag="VGT87_001538"
     CDS             2674..3726
                     /locus_tag="VGT87_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEQ3814375.1"
                     /translation="MQEYSIDITLTHPDDVLALFGSNERHLKLIEAHLGVIVHARTER
                     VQVIGDDEEAVELARLTIKALLVLVGRGMVVNTSDVVTALSMAESHQIDQFMALYEEE
                     IIKDNYGKAIRVKTLGQKTYVDSVKRHDVVFGVGPAGTGKTFLAVTLAVTALKRGQVK
                     RIILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYHILGKEQTTRLMERDVIEIA
                     PLAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFNSKMIVNGDTSQIDLPRNVKS
                     GLIDATQKLQGIKQIDFVYFSAKDVVRHPVVADIIKAYETSSEEMKDLLKPKASDVEA
                     DYQPGLTKYPVIGQEH"
     gene            3772..4347
                     /locus_tag="VGT87_001539"
     CDS             3772..4347
                     /locus_tag="VGT87_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEQ3814376.1"
                     /translation="MDDLTKAIMADEANLSYTERGIFPLYDAPQTARIIIVGQAPGIV
                     AQETKLYWNDRSGIRLRDWLGVDNDTFYHSGLFGIIPMDFYYPGKGKSGDLPPREGFA
                     AKWHPPLRALMPEVELTILVGRYAQDFYLGNKAYKTLTETVRHFEDYLPDYFPLVHPS
                     PRNQLWLAKNPWFEQDLLPILQKRVEAILTK"
     gene            4506..5003
                     /gene="ybeY"
                     /locus_tag="VGT87_001540"
     CDS             4506..5003
                     /gene="ybeY"
                     /locus_tag="VGT87_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEQ3814377.1"
                     /translation="MYIEMIDETGQVSQEIMEQTLDLLNFAAQKTGKEEKEMSVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPETPILFSQEDLAADPSLAEMMAEFDAYIGEL
                     FISIDKAREQSQEYGHSFEREMGFLAVHGFLHINGYDHYTLEEEKEMFTLQEEILTAY
                     GLTRQ"
     gene            4984..5391
                     /locus_tag="VGT87_001541"
     CDS             4984..5391
                     /locus_tag="VGT87_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEQ3814378.1"
                     /translation="MALHDNNTTKRKWKNRTITSSLEFALTGVFTAFKEERNLRSHLL
                     SACLACVAGLFFSISAIEWLFLLLAIFLVITLEIVNSAIENVVDLASDYHFSMLAKNA
                     KDMAAGAVLMISGYAVLTGLIIFIPKIWNIFVH"
     gene            5511..6407
                     /gene="era"
                     /locus_tag="VGT87_001542"
     CDS             5511..6407
                     /gene="era"
                     /locus_tag="VGT87_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEQ3814379.1"
                     /translation="MFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKIM
                     GIYTTETEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVETVLFMVPADEKRGKGDD
                     MIIERLKAAKIPVILVINKIDKVHPDQLLEQIDDFRSQMDFKEVVPISALEGNNVPTL
                     IKLLTDNLEEGFQYFPEDQITDHPERFLVSEMVREKVLHLTQQEVPHSVAVVVESMKR
                     DEETDKVHIRATIMVERDSQKGIIIGKQGAMLKKIGKMARRDIELMLGDKVYLETWVK
                     VKKNWRDKKLDLADFGYNEKEY"
     gene            6427..6903
                     /locus_tag="VGT87_001543"
     CDS             6427..6903
                     /locus_tag="VGT87_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEQ3814380.1"
                     /translation="MPQDYISYIRSKVGHDKIILNFAGGILTNDDGKVLMQLRGDKKT
                     WAIPGGTMELGESSLETCKREFLEETGIEVEAVRLLNVYTHFEEVYPNGDAVQTIVFI
                     YELTAVSDMAIDNFHNEETLKLQFFSHEEIAELESVSAKHRLMLEEYFSDSFAMGH"
BASE COUNT         2269 a         1346 c         1641 g         2307 t
ORIGIN      
        1 acaaaaagct gtagctgaca ccacaagtgt tgcaacctca aacggccttt cttacgctcc
       61 aaaccatgcc tacaatccaa tgaatgcagg gcttcaacca caaacagcag ccttcaaaga
      121 agaagtggct tctgcctttg gtattacgtc atttagtggt taccgtccag gtgatccagg
      181 agatcatggt aaaggtttgg ccattgattt tatggtgccc gaaaattctg ctcttggtga
      241 tcaagttgct caatatgcca ttgaccatat ggcagagcgt ggtatttcat acgttatttg
      301 gaaacagcga ttctatgcgc catttgcaag tatttacgga ccagcctaca catggaaccc
      361 catgccagat cgcggcagta ttacagaaaa ccattatgat catgttcatg tctcctttaa
      421 tgcttaattc atgctatcaa aaaaaactca cgtttaggcg tgagtttttt gacaattctg
      481 acagagtaag ccctataaga ggctgagata cgcttttaat acctgttgtt tttctcaccc
      541 tttagacagt tttaacgtta tgtctaaaag agatggtttt tattagcgca aatatgggtt
      601 taggggtaaa atataggtaa ttaacctgat atgatcatga taaaggtaga agaataccta
      661 ttatttcgtg acaactgagc aatctgccct tacctttgga gtgatgatat cactttgctt
      721 gactattcaa ggaggtcttg aaatgtatta tactagtggt aattacgaag cttttgcgac
      781 acctcgaaaa cctgaagggg tagatcagaa atcggcttat attgttggta ctggtttagc
      841 tggtttagca gcagctgttt tccttattcg cgatgggcat atggctgggg aacgcattca
      901 tctgtttgag gaattgcctt tagcaggtgg ttctttagat ggtattgaaa agcctcatct
      961 tggttttgtg acccgtggtg gtcgtgagat ggaaaatcat tttgagtgta tgtgggacat
     1021 gtatcggtct attccctcac tggaaattcc tggtgcgtct tatttggatg aattttattg
     1081 gttggataag gatgatccta actcatccaa ctgtcgtttg attcacaaga gaggaaatcg
     1141 tgtggatgat gacggccagt atacgctcgg taaacagtca aaagaattaa tccatttaat
     1201 catgaagaca gaagaatctc taggagatca aaccattgaa gagttcttct cagaagattt
     1261 ctttaagagt aatttttgga tctattgggc aaccatgttt gcttttgaaa aatggcattc
     1321 tgctgtagaa atgcggcgct atgcgatgag gtttatccac catattgatg gtttgccaga
     1381 tttcacctct ctcaagttca acaaatataa ccaatatgat tctatggtca aaccgattat
     1441 tgcttaccta gaatcacacg acgttgacat ccaatttgac acaaaagtca ctgatattca
     1501 ggtggaacaa acagctggta aaaaggtagc aaaaaccatc catatgacgg tgtctgggga
     1561 ggctaaggcg attgagctaa cacctgatga tttggttttt gtgaccaatg gttctattac
     1621 tgaaagtagc acatacggta gtcatcacga agtggctaag ccaaccaaag cgttaggtgg
     1681 ttcttggaat ttatgggaaa atctagctgc tcaatcagat gattttggtc atcctaaagt
     1741 gttttaccag gacttgcctg ctgaaagctg gtttgtgtct gccacagcaa ccataaaaca
     1801 cccagctatc gagccttata ttgaacgttt gacccaccgt gatttgcacg atggcaaagt
     1861 gaacactggc ggcatcatca ctattacaga ttctaactgg atgatgagct ttgccattca
     1921 ccgtcaacct cattttaaag aacaaaaaga taatgaaacc actgtctgga tttacggtct
     1981 ttattccaat agtgagggca attacgtcca caagaaaatt gaggagtgta caggtcaaga
     2041 aatcacagaa gaatggttgt accaccttgg ggtacctgtt gataaaatca aggacttagc
     2101 gagtcaggac tatatcaata cagttcctgt ttacatgcct tatattacga gttactttat
     2161 gccacgtgtc aaaggagacc gtccgaaagt tatcccagat ggttcagtca acttggcctt
     2221 tattggtaac tttgcggaat ctccatctcg agatacggtc tttacgactg agtattctat
     2281 tcgtactgcc atggaagcag tgtatagctt cttgaatgtg gaacgaggca ttccagaagt
     2341 ctttaattca gcctatgata ttcgtgaatt gctcaaagcc ttttattacc ttaatgataa
     2401 aaaggcaatc aaggatatgg atttgccaat tcctgcactg attgagaaaa tcggacataa
     2461 aaaaatcaag gataccttta tcgaagaatt gctcaaagat gctaatctta tgtaacatgc
     2521 tttagagtag ttaaagctga taagtttagg cttatcagct tttttgatga tttatagaaa
     2581 agtgataaag gaaaagtctg acgtgaacgt cacataagaa tttaaaattt gttataatgt
     2641 aagcaataaa gatgaaagaa agagggactc tatttgcaag agtattctat tgacattacc
     2701 ttaacccatc ctgatgatgt tttagctctt tttggtagca acgagcgtca tttgaagcta
     2761 atagaagctc atttaggagt tattgttcac gcaagaaccg agcgagtcca agtgattggt
     2821 gatgatgaag aagctgtgga attggctcga ttgaccatca aagccctact ggttctagtg
     2881 ggtcgtggca tggtggtcaa cacatcagat gtggtgacag ctttatctat ggcagaatct
     2941 catcagattg accaatttat ggcgctttat gaagaagaaa tcattaaaga taactatggt
     3001 aaggcaatac gagtcaaaac attaggtcaa aaaacctatg ttgatagtgt caaacgtcat
     3061 gatgtggttt ttggtgtagg acctgcaggg acagggaaaa ccttcttagc tgttacgctt
     3121 gcagtcactg ctctgaaaag aggacaggta aagcgtatta ttttgacgcg tcctgcggta
     3181 gaagctggtg aaagcctagg ttttttacca ggcgatttga aagaaaaggt ggatccttac
     3241 ctcagaccgg tttacgatgc cctttatcac attttaggca aggaacaaac cacacgcctg
     3301 atggaacgag atgtgattga gattgctccg ttagcttata tgcggggtcg tactctggat
     3361 gacgcttttg ttatcttaga tgaggctcaa aacacgacca tcatgcaaat gaagatgttt
     3421 ttgacccgtc ttggttttaa ttctaaaatg attgtcaatg gagatacgag tcagattgat
     3481 ctccctcgaa atgtcaagtc tggtttgatt gatgctactc agaaattaca aggcattaaa
     3541 cagattgatt ttgtctattt ctctgctaaa gatgttgtcc gacatcctgt tgtggctgat
     3601 attatcaagg cttatgaaac gtcctcagag gagatgaagg acttactaaa acctaaggct
     3661 agtgacgttg aagctgacta ccagccaggc ttgacaaaat accctgtgat cggtcaagag
     3721 cactaagacc tttacgatga tgtttaatat ggccttttta aggaggattc tatggatgat
     3781 ttaaccaaag ctattatggc agatgaggct aatctctcct ataccgaaag aggtattttt
     3841 cctttatatg atgctcctca aacagctagg attattattg ttggccaagc tcctggaata
     3901 gtagctcaag aaaccaaact ttactggaat gatcgtagtg gcatccgctt acgagactgg
     3961 ctaggtgtgg acaatgacac cttttaccac tcaggattgt ttggcattat ccctatggat
     4021 ttctattatc ctggtaaggg aaaatccggt gacttaccac ctagagaagg ttttgcggct
     4081 aagtggcacc cgccattaag agcattgatg ccagaagttg aattgacgat tttggttggt
     4141 cgctatgctc aggactttta ccttggtaac aaggcttaca aaaccttaac tgaaacagtc
     4201 cgtcactttg aggattactt gccagactat tttccacttg ttcatccttc tccaagaaac
     4261 caattgtggt tagctaagaa cccctggttt gagcaggatt tattacctat cttgcaaaaa
     4321 cgtgtggagg caattctaac caaataggcc ctgatttacc tagcattccc atttttaagg
     4381 ggcttgtggt ataatgggta aataacaaat gctgattgaa aggataaggg aaattatctg
     4441 tcagataata atgggtgccc aacataccaa atgaggtggg cagcccttcc ctaacaatta
     4501 accttatgta tatcgagatg attgacgaaa cgggacaagt ttcgcaagag attatggagc
     4561 aaacacttga tttgctcaat tttgctgctc aaaagacggg caaagaagaa aaagaaatgt
     4621 ctgtgacctt tgtgaccaat gaaagaagtc atgaactcaa cttagaatac cgagatactg
     4681 atcgtccaac agatgtgatt tctctagaat ataagccaga aacgcctatt ttatttagtc
     4741 aagaggattt ggctgctgat cctagtttgg cagagatgat ggcagagttt gatgcttata
     4801 ttggcgaatt gtttatttct attgacaagg cgcgtgagca gtcccaagaa tatgggcact
     4861 cttttgagcg tgaaatggga tttttagctg ttcatggatt cttacatatt aatgggtatg
     4921 atcactacac ccttgaagaa gaaaaagaga tgtttacctt acaggaagag attttgactg
     4981 cttatggcct tacacgacaa taataccacc aaacggaaat ggaaaaacag aaccattacc
     5041 tcaagcttgg agtttgcctt gacaggggtt ttcacagctt ttaaggaaga acgcaatctc
     5101 agaagtcacc ttttgtctgc ctgtttagct tgtgttgctg gtttattttt tagcatttca
     5161 gccatcgaat ggttattttt acttctagcc atttttttgg tcattacctt agaaattgtc
     5221 aattctgcta ttgaaaatgt agttgatttg gctagtgact atcacttttc tatgctagct
     5281 aaaaatgcta aggatatggc agcaggtgct gttttgatga tttctggcta tgctgtcttg
     5341 acaggactta ttatttttat tcctaaaatt tggaatattt tcgttcatta aaaagaaagg
     5401 aatgggctat cctatgtgat gatggcatag gagaataagg aaaggacaag acggttaagc
     5461 ttagtgacaa gcttgatctg aagccctacc gttaaagccc gtaattatct atgtttaaat
     5521 caggctttgt agccattctt ggtcgtccaa atgttggcaa atccaccttt ttaaatcacg
     5581 tcatggggca aaaaattgcc atcatgagtg acaaggctca gaccacccgt aataaaatta
     5641 tgggcattta caccacagaa acagaacaaa ttgtctttat tgatacgcca ggtattcata
     5701 aaccaaagac cgcccttggt gattttatgg ttgaatctgc ttatagtact ttacgtgaag
     5761 ttgagacggt tcttttcatg gttcctgccg atgaaaagcg tggtaagggt gacgacatga
     5821 ttatcgagcg gctaaaagca gctaagattc ccgttatttt agtcatcaac aaaattgata
     5881 aggttcatcc agaccaactt ttggaacaga ttgatgactt ccgtagccaa atggatttta
     5941 aagaagtggt cccaatttct gccctagaag gtaataatgt gccaacccta atcaagttat
     6001 taactgataa tcttgaagaa ggtttccaat attttccaga agatcagatt actgatcatc
     6061 cagaacgttt ccttgtgtca gaaatggttc gtgagaaggt actgcatttg acgcaacaag
     6121 aggttccaca ttccgtagct gttgttgtcg aatccatgaa gcgagatgaa gagactgata
     6181 aagtgcatat ccgtgcaacc attatggtgg agcgcgatag ccaaaaaggc atcatcattg
     6241 gaaaacaagg agccatgctc aaaaaaatcg gaaaaatggc gcgccgtgat attgaactca
     6301 tgttaggtga caaggtctac ttagaaacgt gggtcaaagt caagaaaaac tggcgggata
     6361 aaaagcttga tttagctgat tttggctata atgagaaaga atactaacat agtaaagcga
     6421 gatcatatgc cacaagacta catttcttat atccgctcca aagttgggca tgataagatt
     6481 attcttaact ttgctggtgg gattttgacc aatgatgacg gcaaggtgct gatgcagttg
     6541 cgtggtgata agaaaacctg ggctattcct ggtggcacta tggaactggg tgagagctct
     6601 ttagagactt gcaaacgtga gttcttagag gaaaccggaa ttgaggttga agctgttcgc
     6661 ttactgaatg tttacactca ttttgaggaa gtttatccca acggtgatgc tgttcagact
     6721 attgtcttta tctatgaact aacggctgtt tctgatatgg ctattgataa ctttcataat
     6781 gaggaaacgc tcaagttaca gtttttctct catgaggaga tagcagagtt agagagtgtc
     6841 tcagccaaac atcgcctgat gttagaggaa tattttagtg atagctttgc tatggggcat
     6901 taagaactct ctaggacagt tggagtattt ttggagattg gcttgttaaa aaaatttttg
     6961 aaagacgatg tttcgagagc ttatgctctc tttttttgct tccaattttt ctgattaagt
     7021 ctgaataatt ttgttaaatg ttaacaggat tttctttcca attgatacta gcagacatca
     7081 aacatttgtt ttagagttat gacgaattgc cacctagcca tgcatgactt aggcaagttg
     7141 aacctaactg tggcaataga cttggaaggg gtgacaagaa tcatttccta aaagaattga
     7201 tgaagtgcct atccattatt cgaattgtaa agttcgtcta agtaaagttg aatttggtct
     7261 cgaatggacc ttaatgattt atgacttgtt gtgtcaataa caccaaaaac tcgataatga
     7321 tttttaaata tttcctcgta attaatatga tttccgatta gaataatatg agcatgagga
     7381 taacacatac gtatttcgtt tagaaggtca tatacttgct tagatttaaa aaagtatgat
     7441 ttatgataaa ataaacctga catttgtatt tataatctaa gtttttccca ctaataattt
     7501 gagtttttag aaagtatctc ttgaggtaat agttaatata gatagatatc atatatccta
     7561 aaa
//
LOCUS       DAUCLR010000086         7226 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.087, whole genome shotgun sequence.
ACCESSION   DAUCLR010000086 DAUCLR010000000
VERSION     DAUCLR010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7226
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.087"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            517..606
                     /locus_tag="VGT87_001544"
     CDS             517..606
                     /locus_tag="VGT87_001544"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEQ3814381.1"
                     /translation="MIIDYCNNKRIKIKRKGLSPLQYKTKSFT"
     gene            complement(644..1567)
                     /locus_tag="VGT87_001545"
     CDS             complement(644..1567)
                     /locus_tag="VGT87_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ3814382.1"
                     /translation="MSEKLVEVKDLEISFGEGKKKFVAVKNANFFIKKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSSGQILYDGKVINGRKSKSEANELIRKIQMIFQDPAASLNE
                     RATVDYIISEGLYNFNLFKTEEERKEKIKNMMAEVGLLSEHLTRYPHEFSGGQRQRIG
                     IARALVMNPEFVIADEPISALDVSVRAQVLNLLKRMQAEKGLTYLFIAHDLSVVRFIS
                     DRIAVIHKGVIVEVAETEELFNNPIHPYTQSLLSAVPIPDPILERQKELVVYHPDQHD
                     YTLDKPSMVEIKPNHFVWANQAEIEKYQKEL"
     gene            complement(1560..2630)
                     /locus_tag="VGT87_001546"
     CDS             complement(1560..2630)
                     /locus_tag="VGT87_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ3814383.1"
                     /translation="MTKENNVILTAKDVVVEFDVRDRVLTAIRNVSLELVEGEVLAFV
                     GESGSGKSVLTKTFTGMLESNGRIANGSIVYRGQELTDLKTNKEWAKIRGSKIATIFQ
                     DPMTSLSPIKTIGSQITEVIIKHQKVSHAKAKEMALDYMNKVGIPNAKKRFEDYPFEY
                     SGGMRQRIVIAIALACRPDILICDEPTTALDVTIQAQIVELLKSLQREYHFTIIFITH
                     DLGVVASIADKVAVMYAGEIVEFGTVEEIFYDPRHPYTWSLLSSLPQLADESGELYAI
                     PGTPPSLYSPIIGDAFALRSEYAMVLDFEKAPPAINVSETHWAKTWLLHPEAPKVQKP
                     EVIQGLHQKILRKMSQQEEGNV"
     gene            complement(2639..3565)
                     /locus_tag="VGT87_001547"
     CDS             complement(2639..3565)
                     /locus_tag="VGT87_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009569326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ3814384.1"
                     /translation="MESIDKSKFRFVERDSEASEVIDTPAYSYWKSVFRQFFSKKSTV
                     FMLVILVTVLMMSFIYPMFANYDFNDVSNINDFSKRYIWPNAEYWFGTDKNGQSLFDG
                     VWYGARNSILISVIATLINITIGVVLGAIWGVSKAFDKVMIEIYNIISNIPSMLIIIV
                     LTYSLGAGFWNLILAFCITGWIGVAYSIRVQILRYRDLEYNLASQTLGTPMYKIAVKN
                     LLPQLVSVIMTMLSQMLPVYVSSEAFLSFFGIGLPTTTPSLGRLIANYSSNLTTNAYL
                     FWIPLVTLILVSLPLYIVGQNLADASDPRSHR"
     gene            complement(3565..5067)
                     /locus_tag="VGT87_001548"
     CDS             complement(3565..5067)
                     /locus_tag="VGT87_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ3814385.1"
                     /translation="MKKYILNRIMRSLVSVVLVTALTYTIVYTLVPTSFIFKQDPNYN
                     KMTTTPDKKVAYENLTFQRMGYVNYFSSKELKDNASKVDSSVTTEATSANKAVYEKYI
                     DSLGNGWQLKRFPTSKQFYAIRNIPIYERVWNFFSNLVVIDHPWKIQDKDNPKLARYI
                     RLEKDKSVGWSLVGSGTKHKYLLYTNGKFPYLHQNFVTLNLGTSYPTYSNIPVLQVIS
                     QGQGRTALQDVTFPSGVTKKSSVDIYSRSYKNPKSLDDITKVNYGKGDSYTKTINNYV
                     DPSMIHNSFVIGFFGVMFSYIVGLPLGLFMARFKNTYFDRFSTATMTFMLALPSIAVI
                     YVVRFLGGMVGLPDSFPMLGASDPKSYILPALILGILNIPTTVIWFRRYLVDLQASDW
                     VRFARSKGLSESEIYRGHLFKNAMVPIVSGVPASIILAIGGATLTETVFAFPGMGKML
                     IDSIKSANNSMIVGLTFIFTVLSIVSLLLGDIVMTLVDPRIKLSTKKGGK"
     gene            complement(5132..7102)
                     /locus_tag="VGT87_001549"
     CDS             complement(5132..7102)
                     /locus_tag="VGT87_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEQ3814386.1"
                     /translation="MKKSKWLAAVSVAILSVSALAACGNKNASGGSEATKTYKYVFVN
                     DPKSLDYILTNGGGTTDVITQMVDGLLENDEYGNLVPSLAKDWKVSKDGLTYTYTLRD
                     GVSWYTADGEEYAPVTAEDFVTGLKHAVDDKSDALYVVEDSIKNLKAYQNGEVDFKEV
                     GVKALDDKTVQYTLNKPESYWNSKTTYSVLFPVNAKFLKSKGKDFGTTDPSSILVNGA
                     YFLSAFTSKSSMEFHKNENYWDAKNVGIESVKLTYSDGSDPGSFYKNFDKGEFSVARL
                     YPNDPTYKSAKKNYADNITYGMLTGDIRHLTWNLNRTSFKNTKKDPAQQDAGKKALNN
                     KDFRQAIQFAFDRASFQAQTAGQDAKTKALRNMLVPPTFVTIGESDFGSEVEKEMAKL
                     GDEWKDVNLADAQDGFYNPEKAKAEFAKAKEALTAEGVTFPVQLDYPVDQANAATVQE
                     AQSFKQSVEASLGKENVIVNVLETETSTHEAQGFYAETPEQQDYDIISSWWGPDYQDP
                     RTYLDIMSPVGGGSVIQKLGIKAGQNKDVVAAAGLDTYQTLLDEAAAITDDNDARYKA
                     YAKAQAYLTDNAVDIPVVALGGTPRVTKAVPFSGGFSWAGSKGPLAYKGMKLQDKPVT
                     AKQYEKAKEKWMKAKAKSNAKYAEKLADHVEK"
BASE COUNT         2260 a         1404 c         1336 g         2226 t
ORIGIN      
        1 gaactacacc ccaaacgtta gacaaaaaaa tctaacgttc ggggtatttt ttatgaaatt
       61 aagtgatgaa agtacgaaat agacatcgtt aagaaagaaa aaaataccta ctatccacca
      121 gagataaagc aagaaataat ggataacgtt ttgatagaag gcccgccaca ataccaagta
      181 tcacttgttt atggcttgtt acatgcagga actctcccaa gttgaatagc acaatataag
      241 aaaaacagtt atactgttga gaaaacaaga gcaagaccat cacaaacagg acgtaaacca
      301 aaagaaacct gaagaaatga ctaaattaga gcggcttcaa gaagaaaatg aatatctcag
      361 aactgaggtt gcttacttaa aaaaaccgag gaactccgtt tgaaagaagc atctctgcaa
      421 agaagaagac aaaaaaacca ttataaaaaa tggacaaaga cgagttctga ttagattttc
      481 ttttaagaac ggctgtattg actcgttcta cctaccatta tcatcgatta ttgcaacaat
      541 aaacgtatca aaataaaacg aaaaggactt agtcctctac aatacaaaac taaatccttt
      601 acttaaaata gagtagccaa cttttggggt cagtgcatct cttttacaat tctttttgat
      661 atttttcaat ttctgcttgg tttgcccaaa caaagtgatt tggtttgatt tcaaccattg
      721 atggcttatc taatgtataa tcatgttggt ctggatgata gacaacaagt tctttttgac
      781 gctctaaaat tggatctggg ataggcacgg ctgataacaa agattgggtg tagggatgaa
      841 tcgggttatt aaacagttct tctgtttctg caacttctac aataacccct ttatggataa
      901 ccgcaatacg atctgaaata aagcgaacga ctgaaagatc atgggcaatg aagagataag
      961 tcaaaccttt ttcggcttgc atccgtttga gaaggtttaa aacctgtgcg cgaacggaaa
     1021 cgtccaaagc tgaaatcggt tcatcagcaa taacgaattc agggttcatt actagggctc
     1081 tagcgatacc gatccgctga cgttgacctc ctgaaaattc atgagggtag cgcgtcaaat
     1141 gctctgatag caaaccaact tcggccatca tgttcttaat tttttcttta cgttcttcct
     1201 ctgttttaaa cagattaaag ttataaagac cttctgaaat gatgtagtca acggttgccc
     1261 gttcattcaa actagcagcg ggatcttgga aaatcatttg aattttacga atgagctcat
     1321 tggcttctga ttttgatttt ctgccattaa ttactttccc atcgtataaa atttgacctg
     1381 aactagtatc gttcaaacca ataatagcac gaccaattgt tgttttacca ctcccagatt
     1441 ctccaactaa agaaaaggtt tctccttttt taataaagaa attagcattt ttaactgcaa
     1501 caaatttttt ctttccttct ccgaaggaaa tttctaggtc ttttacttcg actaatttct
     1561 cagacatttc cttcctcctg ttgtgacatt ttccttaaga ttttttgatg caaaccttga
     1621 atgacttctg gtttttgaac ttttggagcc tctgggtgta aaagccatgt tttggcccaa
     1681 tgagtctcag atacgttaat cgccggaggt gctttttcaa agtctaaaac catagcatat
     1741 tctgagcgaa gtgcaaaggc atctccgata attggtgaat aaagtgatgg aggcgttcct
     1801 ggaatagcgt aaagttcacc agattcatct gccaactgcg gtaagctaga cagcaaactc
     1861 catgtatagg ggtgtcttgg atcatagaaa atctcttcga ctgttccaaa ttcaacaatt
     1921 tctcctgcat acatgacagc cactttatct gcaatgcttg ccacaacacc taaatcgtgc
     1981 gtaataaaga taatggtgaa atgatattct cgttgaagcg acttcaataa ctcaacgatt
     2041 tgagcttgaa tagtcacatc aagggctgtt gttggctcat cacagataag aatatctggg
     2101 cgacaagcta aagcaatagc gataacaata cgttgacgca ttcctcctga atactcaaat
     2161 gggtaatctt caaagcgttt tttggcattt gggataccca ctttattcat gtaatcaagg
     2221 gccatttctt tagctttggc atgacttact ttttggtgct taataatcac ttctgtgatt
     2281 tggctaccga tagttttaat gggactaaga ctggtcattg ggtcttggaa aatcgttgcg
     2341 atttttgagc cgcgaatctt tgcccactct ttatttgttt ttaaatctgt caattcttgc
     2401 ccacgataga caattgaacc attagcaatg cgtccattag actccaacat ccctgtaaag
     2461 gtctttgtta aaacagattt acctgagcct gattccccta caaaagcaag gacttctcct
     2521 tcaacaagtt ccagtgagac gttacggata gctgttaaaa cacgatcacg cacatcaaat
     2581 tctaccacca catctttggc agttaagatt acattatttt cttttgtcat tttaactcct
     2641 atctatgtga acgtgggtca ctggcatcag ccaagttttg tccgacaatg tatagtggta
     2701 acgatactaa aatcaatgtt actaagggaa tccaaaagag gtaggcattt gttgttaagt
     2761 tgcttgaata attagcaatc aaacgtccta aacttggagt ggtggttggt aaaccaatcc
     2821 caaagaagga taagaaggcc tcagaagata cataaactgg tagcatttgt gacaacatag
     2881 tcatgataac tgaaaccaat tgaggcagga ggttcttaac agcaatcttg tacattggtg
     2941 ttcccaaagt ttgactagca aggttgtatt ctaaatcacg gtaacgcaag atttgaacac
     3001 ggatggagta ggcgacacca atccatccag tgatacagaa agctagaatc aaattccaaa
     3061 atcctgcacc taatgaatag gtcaaaacaa tgataataag catagaaggg atatttgaga
     3121 taatgttata aatttcaatc ataactttat caaatgcttt agaaactccc catatggctc
     3181 ctaacactac cccaatggtg atattaatta gtgtcgctat aactgagatt aaaatagaat
     3241 tacgtgcccc ataccaaaca ccatcaaaca gagattgtcc atttttgtcg gttccaaacc
     3301 agtactctgc attcggccaa atataacgct ttgaaaagtc attgatatta ctaacgtcat
     3361 taaagtcgta gttggcaaac attggataaa taaagctcat catcaagact gtcactaaaa
     3421 tcacgagcat aaagactgta gattttttag aaaaaaactg acgaaacact gatttccagt
     3481 aagaataagc aggggtatca atcacttcgg aggcttcact atcgcgctca acaaatcgaa
     3541 atttagattt atcaatcgat tccattactt acctcctttt tttgttgaca atttaatacg
     3601 tggatcgaca agggtcatga caatatctcc tagtaaaagt gaaacaattg acaagacagt
     3661 gaagataaag gttaatccaa caatcataga attgttagca gatttaatag aatcaattaa
     3721 cattttaccc atacccggga aggcaaagac agtctctgtt aaggttgcac caccaatagc
     3781 aaggataata cttgcaggaa cccctgacac aattgggacc atggcatttt tgaagagatg
     3841 ccctctgtaa atttcagatt ctgataatcc ttttgaacgc gcaaaacgta cccaatcgct
     3901 ggcctgaagg tcaacaagat agcgacggaa ccagataacc gtagttggaa tatttaaaat
     3961 tcctaaaata agggctggta aaatataaga tttaggatca gatgccccca gcattgggaa
     4021 actatctggt aaaccaacca tgccacctag gaagcgaaca acatagataa cagcaatact
     4081 tggtaaagca agcatgaagg tcatggtcgc tgttgagaaa cggtcaaaat aggtattttt
     4141 aaaccgagcc ataaataaac caagaggcaa accaacgata taggaaaaca taacaccaaa
     4201 aaaaccaatt acaaaagaat tgtgaatcat tgatgggtcg acataattat tgatggtttt
     4261 agtgtaggaa tctcccttac catagtttac cttggtgata tcatccaatg attttggatt
     4321 tttgtagcta cggctataga tatccactga cgatttctta gtgacacctg atgggaaggt
     4381 cacatcttga agagctgttc gtccttgccc ttgtgaaata acctgaagaa caggaatatt
     4441 gctgtatgtt ggataagatg ttcctaagtt tagggtaaca aagttttggt gaagataagg
     4501 aaattttccg ttagtataga ggagatattt atgttttgtc cccgaaccaa caagtgacca
     4561 gccaactgat ttatcttttt ctagacgaat atacctagct aattttggat tatctttatc
     4621 ctgaatcttc caagggtgat caataacaac tagatttgag aaaaagttcc aaacacgttc
     4681 gtaaatagga atattgcgaa ttgcataaaa ctgtttgcta gtggggaagc gttttagctg
     4741 ccagccattg ccaagagaat caatatactt ctcatagaca gctttgttag cactagttgc
     4801 ttctgtcgtt acactactat ctactttaga agcattatct tttaattctt tgcttgaaaa
     4861 ataattgaca taacccatac gttgaaaagt tagattttca tacgctactt ttttatctgg
     4921 tgttgttgtc attttattat aattagggtc ttgtttaaag ataaagctgg taggtactag
     4981 agtatatacg atggtgtatg ttaaagccgt taccaaaact accgacacta acgatcgcat
     5041 gatacggttt aaaatgtatt ttttcatagt tatcctcaat agttcaaaag aactccctca
     5101 cataaacgag aaagttcttt tgaacatcgt tttatttttc aacgtgatca gctaacttct
     5161 cagcatattt tgcatttgac ttagcctttg ctttcatcca tttttctttt gctttttcgt
     5221 attgttttgc tgtgacaggt ttgtcttgaa gtttcattcc tttatatgct agaggacctt
     5281 tagaccctgc ccaagagaag cccccgctaa atggaacggc tttagtaact cgtggagtgc
     5341 cacccaatgc cacaactgga atatctacgg cattatctgt aaggtaggct tgtgcttttg
     5401 cgtaagcttt atagcgcgca tcgttgtcgt ctgtaattgc tgctgcttca tcaagaagag
     5461 tttggtaggt atcaaggcct gcagctgcca caacatcctt attttgacct gctttgattc
     5521 caagtttttg gataacagat ccaccaccta ctggactcat gatgtcaagg taggtccgtg
     5581 gatcttgata gtctggtccc caccatgatg aaatgatatc gtagtcttgt tgttctgggg
     5641 tctcagcata gaagccttgg gcttcgtgag ttgatgtttc tgtttcaaga acattgacaa
     5701 tgacattttc tttaccaaga gatgcttcaa cagattgttt gaaagactgg gcttcctgaa
     5761 cagttgctgc gtttgcttgg tcaacagggt aatctaattg aactgggaag gttacacctt
     5821 cagctgtcaa agcttctttg gcttttgcaa actcagcttt tgctttttca ggattataga
     5881 aaccatcttg agcatcagct aagttaacgt ctttccattc atcaccaagt tttgccattt
     5941 ccttttcaac ttctgaacca aaatcacttt ctccaatggt cacaaatgtt ggtggcacaa
     6001 gcatgttacg taaggctttt gttttggcat cttgacctgc agtttgggct tggaatgacg
     6061 ctcggtcaaa agcaaactga atagcttgac gaaaatcctt gttgttaaga gctttcttac
     6121 cggcatcttg ttgtgcaggg tctttcttag tgtttttgaa agaagtacgg ttcaaattcc
     6181 atgttaaatg acggatatct ccagtcaaca ttccgtaagt aatgttatca gcatagtttt
     6241 tcttagctga tttgtaggta gggtcatttg ggtaaagtcg tgcaacgctg aactcaccct
     6301 tgtcaaagtt cttgtagaac gaacctgggt ctgaaccatc tgagtaagtc aatttaacag
     6361 attctatccc aacattctta gcatcccagt agttttcatt tttatggaat tccatagatg
     6421 attttgaggt gaaggcgctc aagaagtaag caccattaac aaggattgat gatggatcgg
     6481 ttgtaccaaa atctttacct tttgacttca aaaatttcgc attaactggg aaaagcacac
     6541 tataagttgt ttttgaattc cagtagcttt caggcttgtt caaagtatac tgaacagttt
     6601 tatcgtcaag ggctttgaca ccaacttctt taaaatctac ttcaccattt tggtaagcct
     6661 ttaagttttt tattgaatct tcaacaacgt aaagagcatc tgatttatcg tcaaccgcgt
     6721 gcttcaaacc agtcacaaaa tcttctgctg ttactggggc atattcttca ccatcagccg
     6781 tataccaaga gacaccatcg cgaagagtat aagtataagt cagaccgtct tttgaaacct
     6841 tccaatcttt agcaagtgat ggtactaaat taccatactc atcgttttcc aaaagaccat
     6901 caaccatttg tgttatcaca tcagtcgttc caccgccatt agtcaaaata taatccaatg
     6961 attttggatc gttaacaaaa acgtacttgt aggtttttgt agcttctgag ccacctgaag
     7021 catttttatt accacaagct gccaaagcgg atactgacaa gatcgcaaca cttacagctg
     7081 ccaaccattt acttttcttc ataaaagtca cccctaaact ttatttttca ttttttggca
     7141 attgctaaat gaaattggtt aattgaagac attatatcat aaaactttct taaagaaaat
     7201 aatgaaatga aaaattatct gaatat
//
LOCUS       DAUCLR010000087         7113 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.088, whole genome shotgun sequence.
ACCESSION   DAUCLR010000087 DAUCLR010000000
VERSION     DAUCLR010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7113
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.088"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            253..456
                     /locus_tag="VGT87_001550"
     CDS             253..456
                     /locus_tag="VGT87_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814387.1"
                     /translation="MNYLEYALAYLERELEIIDNDVIEVELPNGGWEFVPNPYYEEGL
                     HDSPYYRSQVAKDILDIKGLLGR"
     gene            545..844
                     /locus_tag="VGT87_001551"
     CDS             545..844
                     /locus_tag="VGT87_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814388.1"
                     /translation="MIEVTFKAESMAGVFDAMREALGMPKTVTKDVVEEEPAPKKKEE
                     SVTLALADIKKMAKAKVEEGKSKDIKEVLKEMNVAKVGELEENQFAEFVEKLEAL"
     gene            844..2001
                     /locus_tag="VGT87_001552"
     CDS             844..2001
                     /locus_tag="VGT87_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015986706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HEQ3814389.1"
                     /translation="MPVENHALLSASSAHRWLYCPMLPRLEADYPSRDTVYTQEGTSA
                     HELSEIKLMYKSGKITKRKFNTLTKAFKENSDFYNEEMEEMTELYTDIVMEHLNAYEN
                     AEIELEKRVDFSDWVPGGFGTSDVVILADGVIEIIDLKYGKGMPVSANQNPQMGLYAL
                     GAYASYDMVYDFDRIKMTIIQPRLDSVSSVDIYVEELLYWADNVVLPMAAQADAGIGD
                     WNLSEKVLQWSPVAAKLVPRAQENWELIDKYDYQEPVYLSDEAVAEILDKASAIKKWV
                     ESVEAYALKEALSGKEVPGYKIVEGRSNRVITDKDKAIDILQDNGFDDEIFKPKELLA
                     MGTLEKLIGKTTFADLLAEVIDKPQGKPVLVKNSDKRPALNSLEQAIKDFE"
     gene            2010..2573
                     /locus_tag="VGT87_001553"
     CDS             2010..2573
                     /locus_tag="VGT87_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HEQ3814390.1"
                     /translation="MIMTPNTTKVVTGKVRLSYVALLEPKAFEGQEAKYSTVILIPKT
                     DKVTIKKIKDAQKAAYEAAKDNKLKGVKWERVKTTLRDGDEEMDTEEHPEYAGHVFMS
                     VSSKTKPQIIDKYKNPVDSADEVYSGVYARVSLNAYAYNTAGNKGISCGLNNVQIVAK
                     GDYLGGRSSADADFDEWNEEEDEDDIL"
     gene            2616..4538
                     /locus_tag="VGT87_001554"
     CDS             2616..4538
                     /locus_tag="VGT87_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEQ3814391.1"
                     /translation="MRHLNIDIETYSSNDIKNGVYKYADAEDFEILLFAYSIDGGEVE
                     CLDLTRQSLPEDIKDMLFDDKVRKHAFNAQFERVCLSRYLGLPYYLDPCQWQCTMVLA
                     QELGLPSSLEKCALYLKLAQEKDNSGKNLIRYFSLPCKPSKANGGRTRNLPEHAPEKW
                     QMFIDYCIQDVVVEMAIAEKLESVPVHDREWGYYACDQRINDRGVALDKELVASALYC
                     KDVKMKSLSGELKAITGLDNPNSRAQLLPWLKEHGYSVNGLTKADVEQELKTAEGELK
                     RVLELKLQTAMSSLKKYEAMERAMCSDGRVHGLLQFYGASRTGRWAGRVVQVQNLARN
                     YIKDLDDAREYVKKRDIDAVEILYDSLNDTLKQLVRTALVAKDCCTFYVSDFSAIEAR
                     VIAWFAGEQWRLDVFSTHGKIYEASASQMFGIPIEEIDKELRQKGKISELALGYQGGP
                     GALKQMGALNMGVKEEELQGLVDDWRRANKKIVQFWKDVQRAAIKAIKSRAPIKLGKL
                     RFRYRKGFLFITLPSGRNLAYARAKVEPGDYGDKIVYEGQGDKAYFTAQETYGGKLVE
                     NIVQATARDILAEALLRIEAAGYGVVFHVHDEAIIEGSGLTIEEVNDLMAQAPEWAGG
                     LPLNSEGYVTKYYMKD"
     gene            4543..6927
                     /locus_tag="VGT87_001555"
     CDS             4543..6927
                     /locus_tag="VGT87_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEQ3814392.1"
                     /translation="MKQEKLIVKSSPLQELHIATGNSRTAKTWKNITLTWQELVERLE
                     KPTVTQETFAEYQKMSRAEKGQAKDVGGFVGGWLKQGKRKNENVQSRSLVALDADSPS
                     KDFLDRLDLLADYAYVLYSTHSHSKKAAKYRLIIPTDRLMMPDEYEPVARYLANQLGM
                     SNFDDTTYQSVRLMFWPSHSRDADFTFKYNDEAFLSVDEVLDTYPDWRDSSFWPESPT
                     HAVKRQREAKKQGDPLSKKGLIGAFCRNYDIRQAIATFLPEVYEEGTTPDRYTYTEGS
                     TANGLVIYDEVFAYSHHGTDPVGDTLVNAYDLVRIHKFGEQDSEAKDTTPTNKLPSSK
                     AMNAFVSDLPEIKDYLMAEALGDFDEELPVEDDRSWLEIDERGEPEVNSYLLATQIIK
                     EVPIYWDGLEFLRYDAKKGIWLPNAEEYLKSYISTKKLGKITKIRHISETVVAIKAQA
                     FSSEVFTESDLNKIVLANGVYDLRDNSFKTKFDPELHARSSHPVVYDPEAACETFEGF
                     LRETVGAENIDFIFEWFGYNFYREYAIQKMLFIYGSGGTGKSTLINILREMIGADNYS
                     AVTLQYLMQERFAKIGLYRKTANFDTDAKPQYLADGATLKMLTGEDTIHADRKNKEPI
                     NFYNYAKLSFAMNELPPMRDFSGGLKRRMMILEMDKVLTQEVKAKYPLDKIMSEVPGI
                     FNRAMEGLRKALSKRDFSISASMRSSVEKWEKGNDVVAMFLEDECELGEDFKVPVRDV
                     YPAYKFYCQDSGYKPLARNSFTQRMNELNFENKNAKMEGKTVRCWIGFRLKGEF"
BASE COUNT         2269 a         1298 c         1733 g         1813 t
ORIGIN      
        1 actttcaaag aagaccagga taagaggaac tttgttaagt tggctgagaa agaatttaaa
       61 ggtgcaactt tgtcatggca agaatttttg tggggtaaaa agaaatgaat atatcactaa
      121 aatttattga tgaaacccta gctggcttaa atgatatttt acgtcagggg ggggctcttg
      181 ctcccaaaac caagccctgg cggacgcagt ttttatacta acagttttaa aacaggtaat
      241 tgaggagaga aaatgaacta tttagaatat gcactagctt atcttgaacg tgagttagaa
      301 attattgaca acgatgttat cgaagttgag ttgcctaacg gaggttggga gtttgtgcct
      361 aatccctatt acgaggaagg tttacacgat agcccatact atcgcagcca ggttgctaaa
      421 gacattcttg atattaaagg acttttgggg aggtgaggct attgattgca tcaaaaaaag
      481 acggcttagg aaaccgtcaa caattataca caattaaatt ataacacaga aagagagaaa
      541 cacaatgatt gaagtaactt ttaaagcaga gagcatggca ggtgttttcg atgctatgcg
      601 tgaagctctg ggtatgccga agacagtaac caaggatgtt gttgaagaag aaccagctcc
      661 taaaaagaaa gaagagtctg taactttggc attagctgat attaagaaaa tggctaaagc
      721 caaggttgaa gaagggaagt caaaggacat caaggaagtc ttgaaagaga tgaacgtcgc
      781 aaaagttggt gaacttgaag aaaaccagtt tgctgagttt gttgagaagt tggaggccct
      841 ctaatgccag tagaaaatca cgcactactg tctgcttcta gtgctcacag atggctttat
      901 tgccctatgc tgccgagatt agaagcagac tatcctagcc gcgataccgt atacactcaa
      961 gagggcacaa gcgcccatga gctttctgaa atcaaactga tgtacaagtc tggtaaaatc
     1021 accaagcgta aatttaacac gttaaccaag gcttttaagg aaaactcaga cttctacaac
     1081 gaagagatgg aagagatgac ggaactttac acagatattg ttatggagca cctaaatgct
     1141 tatgagaacg ccgaaattga acttgaaaaa cgggttgatt ttagcgactg ggtgcctggc
     1201 ggttttggga cttcggatgt tgtcatttta gcggatgggg tcattgaaat tattgatctt
     1261 aagtatggca agggcatgcc tgtgtctgct aaccaaaacc cacagatggg gctgtacgct
     1321 ctaggagctt atgcttccta cgatatggtt tatgactttg accgtatcaa aatgaccatt
     1381 attcagccac gtttagattc ggttagttct gttgacattt acgtggagga gttactctac
     1441 tgggcggata atgttgtctt gcctatggcc gctcaagcag acgcaggcat tggtgactgg
     1501 aacctaagtg aaaaagtatt gcagtggtct cctgtcgcag ctaaattggt accaagagcg
     1561 caagaaaatt gggaattaat tgataaatat gactatcagg agcctgtcta tttatctgat
     1621 gaagccgtcg cagagattct tgacaaagcc tcagctatca agaagtgggt tgagtcagtt
     1681 gaagcctatg ccctgaaaga agcactctcg ggaaaagaag ttccaggcta taagattgtt
     1741 gaaggtagaa gtaatcgagt catcactgat aaagacaaag caattgatat tttacaagat
     1801 aacggctttg acgatgagat ttttaaaccg aaagagctgt tagcaatggg aactcttgaa
     1861 aagttaatag gtaaaaccac ttttgctgat ttattagcag aagtaattga taaaccacag
     1921 ggcaaacctg tccttgttaa aaatagcgac aagcgacccg cattaaatag cttagaacaa
     1981 gcaattaaag attttgaata ggagaaatta taattatgac accaaacaca actaaagtag
     2041 taaccggaaa agtacgccta agctatgtag ctttactaga gcctaaagcc tttgaaggtc
     2101 aagaggctaa gtattcaaca gttatcttaa ttccaaaaac agacaaagtc acaatcaaga
     2161 aaattaaaga cgcgcagaaa gctgcttatg aggctgccaa ggacaataaa ctcaaagggg
     2221 ttaaatggga gcgcgttaag acaacgcttc gtgacggcga cgaagaaatg gataccgaag
     2281 agcacccaga gtacgctgga cacgtgttca tgtcagtgtc aagtaaaact aagccacaaa
     2341 tcattgacaa gtataaaaac cccgttgact cagcggatga agtttactct ggcgtctatg
     2401 ctcgcgtatc acttaatgcc tatgcttaca acacagcggg aaataaagga atctcttgcg
     2461 gattaaacaa cgtccaaatt gttgctaaag gagactacct tggcggccgt tcgtcagctg
     2521 atgcagattt tgacgagtgg aacgaagaag aggacgaaga cgacatttta tagtagaggg
     2581 cctctttaga ggctcctcat ttttaaagga gaggtatgag acatttaaat attgatattg
     2641 aaacctatag ttcgaatgac atcaaaaatg gggtttacaa gtacgctgac gcagaagatt
     2701 ttgagatttt acttttcgct tactctatag atggcggaga agtagagtgt cttgatttga
     2761 caaggcagtc tctacctgaa gacatcaaag atatgttatt tgatgataaa gtccgaaagc
     2821 acgcctttaa tgcccaattc gaaagagttt gtctcagtcg ttacctcggt ctaccttact
     2881 atctagatcc ttgccaatgg caatgcacta tggtgctggc ccaagagttg ggtctgcctt
     2941 caagcttgga aaagtgtgcg ctgtatttaa aattagcgca ggaaaaagat aactcgggta
     3001 aaaacttaat cagatacttt tccctgcctt gcaaaccaag taaagctaac ggtgggagaa
     3061 ctagaaattt accagaacac gcccccgaaa agtggcaaat gtttattgac tactgcatcc
     3121 aagacgttgt tgttgagatg gcaattgccg aaaaactaga gtcagttcct gtgcacgacc
     3181 gtgaatgggg ctactacgca tgcgaccaga gaatcaacga cagaggtgtg gcgcttgata
     3241 aagagttagt tgcttcggcc ttgtattgta aagatgttaa gatgaaaagt ttgtctggtg
     3301 agctaaaggc cataacaggg cttgataatc ctaatagcag ggcgcagttg ctaccgtggc
     3361 taaaagaaca tggctattcg gttaatggtc tgactaaagc ggatgttgag caggagctta
     3421 agacggccga aggagaactt aagagagttt tagaacttaa actacaaacc gctatgtcaa
     3481 gtctaaaaaa atatgaagct atggaaagag ctatgtgctc agacggacga gttcatggac
     3541 tacttcagtt ttacggagct agcaggacag gaagatgggc gggcagagtt gtccaagtac
     3601 agaacttagc taggaattat ataaaggacc tagatgatgc cagagagtac gttaaaaagc
     3661 gtgatattga tgctgtggag attttatacg atagtcttaa cgacacttta aagcagctcg
     3721 tacgaacggc actcgtggcc aaagactgct gtaccttcta tgtctctgac ttctcagcga
     3781 ttgaggctag ggtgattgcg tggtttgctg gagagcagtg gaggcttgac gtgttttcga
     3841 cgcacggaaa aatctatgag gcatccgcta gccagatgtt cggaattcca attgaggaga
     3901 ttgataagga actacgccaa aaaggcaaaa tctcagagtt ggcacttggc tatcaaggag
     3961 gtcctggagc gctcaagcag atgggagctc taaatatggg agtcaaggaa gaggagctcc
     4021 aagggctagt tgatgactgg cgcagggcca ataagaaaat cgtccaattt tggaaagatg
     4081 tgcagagagc cgccatcaaa gctatcaaat cgagagcacc aataaaactt ggaaaactac
     4141 gatttagata ccgtaaaggt ttcctcttta taacattgcc tagcggtagg aacttagctt
     4201 atgcaagagc caaggttgag ccaggcgact atggagacaa aatcgtctat gagggccaag
     4261 gagataaggc ctacttcaca gcgcaagaga cttacggcgg taagcttgtc gaaaatatcg
     4321 ttcaggcgac ggccagggat attctagctg aagcgcttct gaggattgaa gctgcaggct
     4381 atggtgttgt tttccacgtt catgatgagg ctattatcga aggctcaggc ctgacaatcg
     4441 aagaagttaa tgatttgatg gctcaggctc ctgaatgggc ggggggtctt cctttaaata
     4501 gcgaaggcta cgtaacaaag tattatatga aggattagat agatgaagca agaaaaacta
     4561 atagtaaagt cttctccttt gcaagagctt catatcgcaa cgggtaattc acgaacagct
     4621 aagacgtgga aaaatattac gctaacttgg caggagctgg ttgagaggtt agagaaacct
     4681 acagtcaccc aagagacgtt tgcggagtac cagaagatgt ctcgagcaga aaaagggcaa
     4741 gcaaaagacg taggaggttt tgtcggcggg tggctaaagc agggtaaacg gaaaaacgaa
     4801 aatgttcaaa gtaggtcctt ggttgcgctt gacgcagata gcccaagtaa agatttctta
     4861 gataggctag acctgcttgc agactacgca tacgtactct acagtactca cagccactca
     4921 aaaaaagctg ctaagtaccg tcttattatc cctactgacc gtttaatgat gcctgatgaa
     4981 tatgagccag tcgctaggta cttggctaat caactaggca tgtcgaactt tgatgacacg
     5041 acttatcaaa gtgtgcgttt gatgttctgg ccgagccact cgagagatgc tgactttacg
     5101 tttaaatata acgatgaggc ttttctaagt gttgatgagg tgcttgatac atacccggac
     5161 tggcgtgact caagcttctg gccagaaagc ccgacgcacg ctgttaaaag acagcgtgaa
     5221 gctaaaaaac aaggtgaccc acttagtaaa aaagggctta ttggagcctt ttgtcgtaac
     5281 tatgacatta gacaggccat tgcaacgttc ttgcccgagg tttatgaaga aggaacgact
     5341 cctgataggt acacctacac tgagggttca accgcaaatg gcttagtcat ctatgatgaa
     5401 gtcttcgctt atagccatca cgggacagat cccgtggggg atacgcttgt aaatgcatac
     5461 gaccttgttc gtatccataa attcggagag caagatagcg aggctaaaga tactactcct
     5521 actaataagt tgccatcaag taaagcgatg aatgcttttg tctctgactt acccgaaatt
     5581 aaagactatt taatggcgga ggctttaggc gatttcgatg aagagttacc agtcgaagat
     5641 gacagaagct ggcttgaaat tgatgagagg ggcgaacctg aggtcaatag ttatttgcta
     5701 gcaacgcaga ttattaagga ggttccgatt tattgggacg gcttagaatt tttacgctac
     5761 gacgctaaaa aaggcatctg gttgccaaac gcagaggagt atttgaagag ttatatctca
     5821 actaagaaac tcggtaaaat tactaagatt aggcacatta gcgaaaccgt cgtagcgatt
     5881 aaggcacagg ctttctcaag cgaagtgttt actgagagcg atcttaacaa gatagtgcta
     5941 gcgaacggag tctatgactt gagggataac agttttaaga ctaagtttga tccagaattg
     6001 catgcaaggt caagccatcc cgttgtctat gaccccgagg cggcctgtga aacctttgag
     6061 ggttttctta gggagaccgt cggagctgaa aatatagatt tcatctttga gtggttcggc
     6121 tataactttt atcgcgaata tgctattcaa aaaatgctat tcatctacgg cagcggcggt
     6181 actggtaaat caacactgat taatatttta cgtgaaatga taggtgctga taattactca
     6241 gccgtgacac tgcagtacct gatgcaagaa cgctttgcaa aaatcggctt atatcgaaag
     6301 actgctaact tcgataccga tgctaaacct caatacttag cagatggcgc aacacttaaa
     6361 atgttgacgg gggaggatac tattcacgca gaccgtaaaa ataaagagcc gattaacttt
     6421 tacaattatg ctaagctgtc ttttgccatg aatgagctcc cacctatgcg agatttcagt
     6481 gggggactta agcgccgcat gatgatcctc gagatggata aggttttaac gcaggaagtt
     6541 aaggcgaaat acccgctaga taagattatg agcgaggtgc caggtatctt taatagggcg
     6601 atggaggggc ttagaaaggc cttaagtaag agagatttca gtattagtgc cagcatgagg
     6661 tcaagtgtcg agaaatggga aaaaggtaac gatgtcgtgg ccatgttcct tgaagacgag
     6721 tgtgaacttg gtgaagactt caaagttcct gttagggatg tctacccagc ctacaagttc
     6781 tattgtcagg attcaggcta caaacctttg gctaggaatt cgtttacgca aaggatgaat
     6841 gaactgaatt ttgaaaataa gaacgcaaaa atggaaggca agaccgtaag atgttggatt
     6901 ggttttaggc taaaaggaga attttaaagg gttacgtttt tcaacaaaaa aggtaaacag
     6961 gagatataag gttacgtagt tacgcagaat tcagactgat aaaaaatttt ataagttgaa
     7021 aaaaaacgta actacataac tttcctgaaa aacctttcct gtttaagtca atttacataa
     7081 ctaacgcaac ttttatgcaa cctcaaaaaa cct
//
LOCUS       DAUCLR010000088         7036 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.089, whole genome shotgun sequence.
ACCESSION   DAUCLR010000088 DAUCLR010000000
VERSION     DAUCLR010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7036
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.089"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..1220
                     /locus_tag="VGT87_001556"
     CDS             <1..1220
                     /locus_tag="VGT87_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEQ3814393.1"
                     /translation="KKLIFSKNAVYQLKQDVVQSTQFYNQIPSNPNLYQETCAYKDSD
                     LTLPAGRLGVNQPLLIKSLVLNKESLPVFELADGTYVEANRQLIYDDIVLNQVDIDSY
                     FWTQKKLRLYSAPYVLGTQTIPSSFSFAQKVHATQMAQTNHGTYYLIDDKGWASQEDL
                     VQFDNRMLKVQEMLLQKYNNPNYSIFVKQLNTQTSAGINADKKMYAASISKLAPLYIV
                     QKQLQKKKLAENKTLTYTKDVNHFYGDYDPLGSGKISKIPDNKDYRVEDLLKAVAQQS
                     DNVATNILGYYLCHQYDKAFRSEIKALSGIDWDMEQRLLTSRSAANMMEAIYHQKGQI
                     ISYLSNTEFDQQRITKNITVPVAHKIGDAYDYKHDVAIVYGNTPFILSIFTNKSTYED
                     ITAIADDVYGILK"
     gene            1217..2503
                     /gene="tilS"
                     /locus_tag="VGT87_001557"
     CDS             1217..2503
                     /gene="tilS"
                     /locus_tag="VGT87_001557"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEQ3814394.1"
                     /translation="MMTYQEIFNEIKNKAYFKNHRHVLIAVSGGVDSMNLLHFLYLFQ
                     DKLTIRIGIAHVNHKQRSESDSEEAYLKCWAKKHDIPIYVSNFEGIFSEKAARDWRYA
                     FFKSIMLKNNYSALVTAHHSDDQAETILMRLIRGSRLRHLSGIKSVQPFANGQLIRPF
                     LTFSKKDLPEIFHFEDSSNRELSFLRNRVRNNYLPLLKQENPRFIQGLNQLALENSLL
                     FQAFKELTNHITTTDLTEFNEQSKSIQYFLLQDYLEGFPDLDLKKSQFTQLLQIIQTA
                     KQGYYYLKKDYYIFIDKFSFKITKIVPKTELVKEEKMLEYDSNLCYRDYYFSFMPKSN
                     EDQGQVSIPLFSLSSIKLRSRQSGDYISFGHFSKKIRRLFIDEKFTIAERQNAIIGEQ
                     DEQIIFVLIGNKTYLRKACKHDIMLAKLYIDKLEKG"
     gene            2508..3050
                     /gene="hpt"
                     /locus_tag="VGT87_001558"
     CDS             2508..3050
                     /gene="hpt"
                     /locus_tag="VGT87_001558"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEQ3814395.1"
                     /translation="MLEQDIQKILYSENDIIRKTKKLGEQLTKDYQEKNPLMIGVLKG
                     SVPFMAELMKHIDTHVEIDFMVVSSYHGGTSSSGEVKILKDVDTNIEGRDIIIVEDII
                     DTGRTLKYLRDMFKYRKANTIKIATLFDKPEGRVVKIEADYVCYNIPNEFIVGFGLDY
                     AENYRNLPYVGVLKEEVYSK"
     gene            3072..5051
                     /gene="ftsH"
                     /locus_tag="VGT87_001559"
     CDS             3072..5051
                     /gene="ftsH"
                     /locus_tag="VGT87_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEQ3814396.1"
                     /translation="MKNNKNNGFVKNSFIYILMIIVVITGFQFYLKGTSTQSQQISYS
                     KLIKHLKAGDIKSLSYQPSGSIIEVKGKYEKPQKVTVNSGLSFLGGRASTQVTEFSSL
                     VLPSDTILKEMTAAADKNGTELTVKQESSSGTWITFLMSFLPIVIFAAFMMMMMNQGG
                     GGARGAMSFGKNKAKSQSKGDVKVRFTDVAGAEEEKQELVEVVDFLKNPKKYKSLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAERAIIFIDEIDAVGRRRGAGMGGGNDEREQTLNQLLIEMDGFEGNENIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILRVHAKNKPLANDVNLKVVAQQTPGFV
                     GADLENVLNEAALVAARRNKIKIDASDIDEAEDRVIAGPSKKDRTISQKEREMVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDLKEQLAGLMGGR
                     VAEEIVFNAQTSGASNDFEQATQIARAMVTEYGMSEKLGPVQYEGNHAMMPGQISPEK
                     AYSAQTAQMIDDEVRELLNQARNQAADIINENRDTHKLIAEALLKYETLDAAQIKSIY
                     ETGKMPVDLETDDNEAHALSYDEIKNKMTESESAE"
     gene            5377..6768
                     /locus_tag="VGT87_001560"
     CDS             5377..6768
                     /locus_tag="VGT87_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEQ3814397.1"
                     /translation="MTIFRKKKKYSNKTEMQRHFKVIDLIFLGLGSMVGTGIFTVTGI
                     GAAKYAGPALTISIIISAIAIGILALFYAEFASRMPSNGGAYSYVYATLGEFPAWLVG
                     WYIIMEFLTAISSVAVGWGSYLKGLLANYNIVLPNALNGTFNLKNGTYIDILPVLVMF
                     FVTGIVLMNSKLALRFNSFLVILKFSALALFIFVGIFFIDHNNWSHFAPYGVGQITGG
                     KTGIFAGASVMFFAFLGFESISMAVDEVKEPQKTIPKGIILSLIIVTALYIVVTTILT
                     GIVHYTKLNVPDAVAFALRNIRLYWAADYVSIVAILTLITVCISMTYALARTIYSISR
                     DGLLPKSLYTLTKKNKVPQNATLVTGLLAMICAGIFPLSSLAEFVNICTLAYLIILSG
                     AIIKLRRIEGEPKANEFKTPLVPFLPMLAIIICLSFMSQYKAFTWIAFAIATIIGTLI
                     YLAYGYTHSIENK"
BASE COUNT         2441 a         1025 c         1255 g         2315 t
ORIGIN      
        1 aaaaaaaact tatattttca aaaaatgctg tttatcaatt gaaacaagat gtcgttcaat
       61 caacacaatt ctataatcaa ataccctcta atccaaatct ttatcaagaa acgtgtgcct
      121 ataaagacag tgatttaact ctaccagcag gaagattagg tgtaaatcaa ccattactta
      181 ttaaatcgct tgtgcttaac aaagaatctt taccggtttt tgagttagct gatggtacct
      241 atgttgaggc taatcgacaa ttgatttatg acgatattgt acttaatcaa gtagatatag
      301 atagctattt ttggacacaa aagaaactta ggctttattc agccccttat gttttaggta
      361 cgcaaacaat tccttcttct ttttcatttg ctcaaaaagt tcatgccact caaatggcac
      421 aaacaaacca tggaacttat tatcttattg atgataaggg ctgggcatca caagaagatc
      481 tagttcaatt tgataaccgc atgttaaaag tccaggaaat gctcttacaa aaatataata
      541 acccaaatta ttcaattttt gtaaagcaac tcaacacaca aacaagtgct ggtattaatg
      601 ctgataaaaa aatgtatgct gcaagtatct cgaagttagc accactttat attgttcaaa
      661 aacaattaca aaaaaagaaa ttagcagaga ataaaacttt gacttatact aaagatgtta
      721 atcattttta tggagactat gatccattag gaagtggtaa aattagtaaa atacctgata
      781 ataaagatta tcgtgttgaa gacctactga aagctgtagc acaacaatcg gataatgtag
      841 caactaatat tttaggttat tatctatgtc atcagtatga taaagctttc cgctcagaga
      901 taaaagcttt atcaggtatc gattgggata tggagcagcg cttattaact tctcgttcag
      961 ctgcaaatat gatggaagct atttatcatc aaaaaggcca aattatttct tacctttcaa
     1021 ataccgaatt tgatcaacaa cgtatcacaa aaaatattac tgttccagtt gcacataaaa
     1081 ttggtgatgc ttatgattat aaacatgacg ttgctattgt ttacggtaat actccattta
     1141 ttttgtctat ttttacaaat aaatcaacct atgaagatat tacggctatt gcagatgacg
     1201 tttatggtat tttaaaatga tgacctatca agaaattttt aatgaaataa aaaataaagc
     1261 ctactttaaa aatcatagac atgtgttaat agccgtatct ggtggagtag attcaatgaa
     1321 tttattgcac tttttgtacc tgtttcaaga caaattgaca attcgtattg gtattgctca
     1381 tgttaatcac aaacaaagat cagaatcaga ttcagaagaa gcttatttaa agtgttgggc
     1441 taaaaaacat gacataccta tttatgtttc taattttgaa ggaatatttt cagaaaaagc
     1501 agcacgtgat tggcgctatg ctttttttaa gagtattatg ttaaaaaata actattcagc
     1561 tttagtaaca gctcaccatt cagatgatca agcagaaact attttgatga ggctaattag
     1621 aggaagccga ttgaggcact tgtcgggtat aaaaagcgtg caaccatttg ccaatggtca
     1681 acttataaga ccttttttaa ctttctcaaa aaaggacttg ccagaaattt ttcattttga
     1741 agattcttca aatagagaat tatcattttt aagaaataga gttagaaata attatttacc
     1801 actgttaaag caggaaaatc ctaggttcat tcagggatta aatcaattag cacttgaaaa
     1861 tagcttactt tttcaagctt ttaaggagct aacaaatcat attactacca ctgatttaac
     1921 agaatttaat gagcaatcta agtcaattca atactttttg ttacaagatt atttagaagg
     1981 ttttccagat ttagatttaa aaaaatctca atttactcaa ttattgcaaa ttatccaaac
     2041 tgctaaacaa ggctattatt atctcaaaaa agactattat atattcattg ataaattctc
     2101 ttttaaaatt acaaaaatcg ttcctaagac tgagttagtt aaagaagaaa aaatgttaga
     2161 atacgatagt aacttatgct acagagatta ttatttttca tttatgccaa aaagtaacga
     2221 agatcaagga caggtaagta tacctttatt tagtttatcg tctattaaat taagaagtcg
     2281 ccaatctgga gattatattt cttttggtca cttttcaaaa aaaataagac gtttatttat
     2341 agatgaaaaa ttcacaatag ctgaacgtca aaatgctatc attggagagc aagatgagca
     2401 gattatattt gtattgatag gtaataaaac ttatttgaga aaagcttgta aacatgatat
     2461 aatgttggct aaactgtata ttgataaatt agaaaaaggg tgacagcatg ctcgagcaag
     2521 atattcaaaa aattctttac tctgaaaatg atattattcg gaaaactaaa aaattaggag
     2581 aacaattaac aaaagattat caagaaaaaa atccgcttat gattggtgta ttaaaaggat
     2641 cagttccttt tatggcagaa ctaatgaaac acatcgatac tcatgttgaa attgatttta
     2701 tggtagtatc aagttatcat ggcggtacat ctagcagtgg tgaagttaaa attttaaaag
     2761 atgtagatac caatattgaa ggaagagata tcataattgt tgaagatatt attgacaccg
     2821 gtcgcacctt aaaatattta cgagatatgt ttaagtaccg taaagctaat actatcaaaa
     2881 ttgcaacact gtttgataaa cctgaaggtc gtgttgttaa aattgaagca gattatgttt
     2941 gttacaatat tccaaatgaa tttatcgtag gctttggttt agattacgca gaaaattatc
     3001 gcaatcttcc ttacgtaggt gtattaaaag aagaagtcta ttcaaaatag aaaaaggtca
     3061 atgtttactg tatgaaaaat aataaaaata atggttttgt taaaaattct tttatatata
     3121 tattgatgat tattgttgtc atcacaggat ttcaatttta tcttaaggga accagcacac
     3181 aaagccaaca gattagttat tccaaattaa ttaaacatct taaggctgga gatataaaat
     3241 ctttaagtta tcagcctagc ggtagtatta ttgaagttaa agggaaatat gagaaacctc
     3301 aaaaagtgac agttaattca ggactatcat ttttaggtgg aagagcttca acacaggtaa
     3361 cagagttctc atctttagta ttacctagtg ataccatctt gaaagaaatg acagcagcag
     3421 ctgataaaaa tggaacagag cttacagtta agcaggaaag ttcaagtggt acttggatta
     3481 ccttcttaat gagcttttta cctattgtta tttttgctgc tttcatgatg atgatgatga
     3541 atcaaggtgg tggaggagct cgtggtgcta tgagtttcgg caaaaataag gcaaaatctc
     3601 aatcaaaagg tgatgttaaa gtgagattta cagacgttgc tggtgccgaa gaagaaaaac
     3661 aagaattagt agaagttgtt gattttttga aaaatcctaa aaaatataaa tcactaggag
     3721 cacggattcc agcgggagtt cttctagagg ggcctccagg tactggtaaa acattactag
     3781 ctaaagctgt agcaggtgaa gctggagtgc catttttcag tatttcaggt tcagattttg
     3841 tcgaaatgtt tgtcggtgta ggtgctagtc gtgttcgttc tttatttgag gatgctaaaa
     3901 aagctgagcg tgcgatcatc tttatcgatg aaattgacgc tgtaggtcgt cgtcgtggtg
     3961 ctggcatggg aggcggtaac gatgaacgag aacagaccct taaccaatta ctcattgaaa
     4021 tggatggttt tgagggtaat gagaacatta tcgttattgc tgcaactaac cgtagtgatg
     4081 ttttagatcc agctttacta agaccaggtc gttttgatcg taaggtttta gttggtcgcc
     4141 ctgatgttaa aggacgtgaa gccattttac gtgttcatgc taaaaataaa ccacttgcaa
     4201 atgacgttaa tttgaaagta gtagcccaac agactccagg ttttgtaggt gctgatcttg
     4261 aaaatgtttt aaatgaagct gcgttagttg ctgctcgcag aaataaaatt aaaattgatg
     4321 ctagtgatat tgatgaagct gaagatcgtg ttattgctgg accatcaaag aaagatcgca
     4381 ctatttcgca aaaagagcga gaaatggttg cttatcacga agctggtcat actattgttg
     4441 gtttagtttt atctaatgcg cgtgtagttc acaaggtaac tattgtacct cgtggacgag
     4501 caggtggtta tatgatcgct cttccaaaag aagatcaaat gcttctttct aaagaagatc
     4561 tgaaagagca attagcagga ttgatgggtg gtcgtgttgc tgaagaaatt gtttttaatg
     4621 ctcaaacatc aggtgcgtca aatgactttg agcaagctac tcagatagca agggccatgg
     4681 ttacggaata tggtatgagt gaaaagcttg gaccagttca atatgaaggt aaccatgcga
     4741 tgatgccagg acagatctct cctgaaaaag cttactctgc ccaaacagct caaatgattg
     4801 atgatgaggt tcgtgaactc ttaaatcaag ctcgtaatca agcagctgat atcatcaatg
     4861 agaatcgaga tactcataaa ttgattgctg aagctcttct caaatatgaa actctagacg
     4921 ctgcacaaat taaatcaatt tatgaaactg gtaaaatgcc agttgattta gaaactgatg
     4981 ataatgaagc tcatgcgtta tcttatgacg aaattaaaaa taaaatgaca gaatctgaat
     5041 ctgcagaatg agataaaaaa gctgaactta tagattaagt tcagcttttt tatcaacaca
     5101 atatccttgt aaacgttatg aattgtaata ggtgcctaat tgctagaaat gagaagatga
     5161 catacttttt ttctttatta cttaaagtga gaaagacttt ggtatttaaa actagaaaat
     5221 tgttaaagcc atttgaaaaa atctattagt gttttcttag aataatagaa gaacagaaga
     5281 tagcaaaagt ttcagtcaac caataagatg aaatttacaa tcattactaa ctatgataca
     5341 cttagtagaa aatcatttac tatagaaggt gataacatga ctatttttcg taaaaaaaag
     5401 aaatattcca ataaaacaga aatgcaaagg cacttcaaag ttattgattt aattttttta
     5461 ggcctaggtt caatggttgg aacaggtatc tttacagtta ctggcatagg cgctgctaag
     5521 tatgcaggtc ctgctttaac aatatcgatt atcatttccg cgattgctat tggaatttta
     5581 gccttatttt atgcagaatt tgcgtctcgt atgccttcta acggtggtgc ttatagttat
     5641 gtttatgcaa ccttgggaga gtttccagca tggcttgttg gctggtatat tattatggaa
     5701 tttttaacag ctatatctag tgttgctgtt ggctggggaa gttatttaaa aggattactt
     5761 gccaattata atatagtatt accaaacgct ttaaatggca cgttcaatct taagaatgga
     5821 acttacatag atattcttcc tgttttagtg atgtttttcg tgacagggat tgtcctaatg
     5881 aattcgaaat tagccctgag attcaatagt tttttagtta ttttaaaatt ttcagcactg
     5941 gctttattta tctttgtagg catttttttt attgatcaca ataattggtc tcattttgct
     6001 ccatatggtg taggacaaat tactggaggt aaaacaggta tatttgctgg tgcttctgtt
     6061 atgttctttg catttttagg atttgaatct atttcaatgg cagttgatga agtgaaagaa
     6121 ccacaaaaaa caatccctaa aggcatcatt ttatctttga ttattgtaac agcactctat
     6181 atagttgtca ccactatttt aacgggaatt gtacactaca ctaaattaaa tgttccagac
     6241 gcggtggctt ttgctttaag aaatattaga ctttattggg cagctgacta tgtgtcgata
     6301 gttgctattt tgactcttat tacggtatgt atttctatga cctatgcatt agctagaaca
     6361 atttacagta ttagtagaga tggtttatta cctaaatcac tttatactct cactaaaaaa
     6421 aataaagtgc ctcaaaatgc tactcttgtc actggactac ttgctatgat ttgtgcggga
     6481 atatttcctt tatcgagtct agcagagttt gttaatattt gtactttagc atatttaatt
     6541 attctgtcgg gtgcaattat taaactcaga agaatagaag gagaaccaaa agcaaatgaa
     6601 tttaaaactc ctctagtacc attcctacca atgttggcaa ttatcatttg tctatcattt
     6661 atgagccaat ataaagcttt tacttggatt gcttttgcta ttgccacaat tattggaaca
     6721 ttaatctact tagcttatgg ttatactcat tcaatagaaa ataaataatg ttttgataat
     6781 aagatttgac attacattat agactataat gtttagaagt aaatgagcag taaaatcaat
     6841 acctacttac ttatcaaagt tgtaatattt aaaattaccc aacccctaga gaattgatta
     6901 gtctctaggg gttgattggt acaatgacat agactctaaa acgattttaa tactagatat
     6961 atagatataa atttcaaaaa agaaaattag ttcatttttg ttatacaata aaatttgtta
     7021 tttagcagta atataa
//
LOCUS       DAUCLR010000089         7025 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.090, whole genome shotgun sequence.
ACCESSION   DAUCLR010000089 DAUCLR010000000
VERSION     DAUCLR010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7025
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.090"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            17..931
                     /locus_tag="VGT87_001561"
     CDS             17..931
                     /locus_tag="VGT87_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEQ3814398.1"
                     /translation="MNLILLFACILGLTLIFYLYQGSNKFGLSDVSQFVPKISLFHHK
                     KTAKKETTKLKKTHFDSSKSQKKAHSKLTWTKQETPVKIPILMYHAIHVMSPEETANA
                     NLIVNPDLFDQQLQKMKDEGYYFLSPEEVYRALSNNELPAKKVVWLTFDDSMIDFYNV
                     AYPILKKYDAKATNNVITGLTEMGSAANLTLKQMKEMKQFGMSFQDHTVNHPDLEQAS
                     PDVQTTEMKDSKDYLDKQLNQNTIAIAYPSGRYNDTTLQIAARLNYKLGVTTNEGIAS
                     AANGLLSLNRIRILPNMSPENLLQTMEP"
     gene            complement(1048..2283)
                     /locus_tag="VGT87_001562"
     CDS             complement(1048..2283)
                     /locus_tag="VGT87_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEQ3814399.1"
                     /translation="MIKRWVILFFMVLTCSGFGKTVLAADFQVGAEHAIVVEADSGRV
                     LYEKDAKTPDAIASLTKLVTAYLVLDKVKSGQLQLSDQVNLSDYAFELTKDRSLSNVP
                     FDKKTYSVQDLLTATLVASSNSAAIALAEKVAGSELHFVNQMREQLSHWGITSGKILN
                     ASGLPNEVLKDNRYPGSALEEENTLSAQDVAVVTMHLLEDFPEILEITKQTEVNFAGN
                     SIKSFNQLLPGMAKGRAGVDGLKTGTTDLAGHCLVVTSIENGMRVITVILNADGSDKN
                     QNTRFEQANRLLDYVARTYCRRKILKKGSLVSERSLPIQDGQVKELPVSVAEDVTIIL
                     QQGEQVPKPKQFMISETSLLAPITKGEVVAYLTSPRITDQSVRYLKEPKRIPLKASQS
                     LKKASDLQLWWRDFLEKRR"
     gene            complement(2441..3628)
                     /locus_tag="VGT87_001563"
                     /pseudo
     CDS             complement(2441..3628)
                     /locus_tag="VGT87_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003106750.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(3631..3852)
                     /locus_tag="VGT87_001564"
     CDS             complement(3631..3852)
                     /locus_tag="VGT87_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEQ3814400.1"
                     /translation="MNIGFLEHTARDIIREAKRIAIKKFEEAREIDQNAVQLSKSPFD
                     NRRLGIAPKDIVEELIGILLSEESLESEN"
     gene            3939..4082
                     /locus_tag="VGT87_001565"
     CDS             3939..4082
                     /locus_tag="VGT87_001565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814401.1"
                     /translation="MGISPTGLLRWQPERSEVSLSLFVVMADREPLYFIANNYRSYHG
                     VSH"
     gene            complement(4153..4371)
                     /locus_tag="VGT87_001566"
     CDS             complement(4153..4371)
                     /locus_tag="VGT87_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ3814402.1"
                     /translation="MYPRIRNLREDNDFTQKFVANLLSFSHANYAKIERGEVALMADV
                     LVQFSKLYNVSIDYLLGQTDYPYRFHNK"
     gene            complement(4563..5285)
                     /locus_tag="VGT87_001567"
     CDS             complement(4563..5285)
                     /locus_tag="VGT87_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HEQ3814403.1"
                     /translation="MKSYLCSNFLKIKNTSYLLIHILAALLFPLLLFFYWSQRGGLQS
                     RTVLFSYFQIVGVLLPFVASIVCIQLKNLEESSGKYKYLLGYSQSNYKPFIVELVFLW
                     LCYCIVLIISITIFILLLKTIGINVSLRLLILNSLIYIIFAYVTYLINHIISYIFSTG
                     VALGISMVGVIAAAFCETSLGDKVWFLIPWAWLLRISDTLYNQQKMAIVPLIVIFFVS
                     CTVALLHIRIFKSWNQDCLTSS"
     gene            complement(5288..6037)
                     /locus_tag="VGT87_001568"
     CDS             complement(5288..6037)
                     /locus_tag="VGT87_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HEQ3814404.1"
                     /translation="MVQIFLSNLLKIKNTSISKLILIFPIICVMLSLLFSALGGENIL
                     RLTAETTVNQWGVIWINVIIAVNSGLLNKLELDSNKYSVFLSRDVDLKKVEYARVLLI
                     ALINLIISMILSLMLIVISFVLPTPSLISIGRILLTILLIWLTTLWQIPFILWLSRKI
                     NVYFAMIINIISPLIIGTSFSLLNKWYLFPYDWSLKLLEPMTRMRINSIPFGAEFVPD
                     YSQIFISLFLGIAFFILLTNLFAISFKKQVK"
     gene            complement(6043..6732)
                     /locus_tag="VGT87_001569"
     CDS             complement(6043..6732)
                     /locus_tag="VGT87_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HEQ3814405.1"
                     /translation="MLKIQNLKKSYGKRTILNNVNMNIPKGKVYALIGPNGAGKSTIM
                     KILTGLVSKTSGSIIFEGREWSRRDLRKIGSIIEEPPLYKNLSAYDNMKVVTTMLGVS
                     ESTILPLLNKVGLGNIDKRPVKQFSLGMKQRLGIAISLINSPKLLILDEPTNGLDPIG
                     IQELREIIESFKSEGMTIMISSHILSEVEHLADFIGFIYEGKIILEKEYDGSENLEEL
                     FNNQILFEKRR"
     gene            complement(6738..>7025)
                     /locus_tag="VGT87_001570"
     CDS             complement(6738..>7025)
                     /locus_tag="VGT87_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiopeptide-type bacteriocin biosynthesis
                     protein"
                     /protein_id="HEQ3814406.1"
                     /translation="PIDLPIEYMKILNGVTSALDRYDFFQEMDRYLMSYMKEYNRFGG
                     TNDIYNTKFNLVGSFLHLSMNRLNGIDREFEEKVYCFAYYTLNAQQYIEWE"
BASE COUNT         2374 a         1349 c          985 g         2317 t
ORIGIN      
        1 aaaagccttc actctcatta accttatact attatttgcc tgcattcttg gtttgaccct
       61 cattttctat ttataccaag ggtctaacaa attcggcttg tcagatgtct ctcaatttgt
      121 acctaaaata agtctttttc atcacaaaaa gacggcaaaa aaagaaacga caaaacttaa
      181 gaagactcat ttcgattcct caaaatctca aaaaaaggcc cattctaaat taacttggac
      241 caagcaagaa acacctgtca agatccccat tttaatgtat catgctattc atgtaatgtc
      301 ccctgaagag acggccaatg ctaatttgat tgtcaaccca gatctctttg atcaacaact
      361 tcaaaaaatg aaagacgagg gctactattt tttgagtcct gaagaggtct accgcgctct
      421 ttcaaataat gaattgcctg ctaaaaaagt tgtatggcta acatttgatg acagtatgat
      481 tgatttctac aacgttgctt acccaatctt aaaaaaatat gatgctaaag ccaccaataa
      541 tgttattaca ggactaaccg aaatgggaag cgctgccaat ctgactctta agcagatgaa
      601 agaaatgaaa caattcggta tgtctttcca agatcataca gtgaatcatc ctgatctaga
      661 gcaggctagt cctgatgttc aaacaactga aatgaaagat tcaaaggact atcttgataa
      721 gcaattaaac caaaacacta ttgccattgc ttatccatct ggtagatata atgacactac
      781 cttacaaatt gcagctagac ttaactataa attaggtgtt accactaacg aaggaatagc
      841 tagcgcggct aatgggcttc tttctcttaa caggattcgt atcctcccaa acatgagtcc
      901 ggaaaatctt ttacaaacaa tggaaccata acatcaattt ttttatcatt ccaaatcata
      961 ctcactgtaa actaaaaaga cagttatgaa aaaattcttt cataactgtc tttttttgat
     1021 tgttttaaaa ttattttatt cttatcttta acgtcttttt tcaagaaaat ctcgccacca
     1081 cagctgtaag tcagaggctt ttttaagaga ttggctagct ttaagaggaa tacgcttagg
     1141 ttcctttaaa tacctcactg actgatctgt tattcttgga gacgtcaaat aagcaaccac
     1201 ttcaccttta gtaatcggtg ctagtaaaga agtctctgat atcataaatt gtttaggttt
     1261 gggaacttgt tcaccctgtt gcaaaatgat ggttacatct tcggcaacag ataccggtag
     1321 ttcttttact tggccatctt gaatagggag cgaccgctcc gagacaagac ttcctttttt
     1381 aagaatctta cgacgacaat aagttctagc cacatagtcc aacaaacgat ttgcttgctc
     1441 aaaccgcgtg ttttggtttt tatcagatcc atcggcattt agaataacgg taatcactcg
     1501 cataccattt tcaatagaag ttaccacaag acaatgacct gccaagtctg ttgtcccagt
     1561 ctttaaacca tccactcctg ctctaccctt agccatacct ggtaagagtt gattaaaact
     1621 tttaatagaa ttgccagcaa aattaacttc tgtttgtttg gtgatctcta aaatctcagg
     1681 aaaatcttct aacaaatgca tcgtcacaac tgcaacatct tgagcactca aggtattttc
     1741 ttcttccaac gcagaaccag gataacgatt atcctttaac acctcatttg gcagacctga
     1801 ggcgttgagg attttaccag aagtaatccc ccaatgagat agctgttctc tcatttgatt
     1861 tacaaaatga agctctgaac ccgccacttt ttcggctaac gcaatagcag cgctatttga
     1921 gcttgctacc aaagtagctg tcaacaggtc ttgaactgaa taagtttttt tgtcaaaagg
     1981 tacattactt aaagacctat cttttgttaa ctcaaaggca taatctgaga gattaacttg
     2041 atctgataat tgcagctggc ctgatttaac cttatctaaa accagataag ccgtcaccaa
     2101 tttggtcaaa gaggcaatag catctggcgt cttagcgtct ttctcataca gaactctccc
     2161 agagtctgcc tctacgacaa tcgcatgttc tgctcccact tgaaaatcag ctgcgagaac
     2221 tgttttacca aaccctgaac aagttaatac cataaaaaaa aggataaccc acctttttat
     2281 cattatatct ttcatagtta ctattataac aaaaattctc agaaatttgg aaggaagtat
     2341 agtggcacac acctttagta gcttaaacaa gctcataaag caagtaccct gtgacaataa
     2401 cacatcctgt tatatgggcg atataataag cttcgttata tcaattagtt gtttggcagg
     2461 aattttggta taaaatggaa tctaatcctg aacttattac attttgggtt atttcttcag
     2521 atactgtttt atctaatgca ttaaaccagt gactatatgt attcaaagtt gtagacttat
     2581 cagcatgtcc catacacttt gcaacgtaca gaatgtcttt tttcaaaaca ttaataagat
     2641 aggaagcatg actatgtcgc aagacttttc ccgttatcac tggtactcct acttcttgag
     2701 ctttgtgctt aataactcga caaatcgttg atttacaaaa tggttcacca aaccgtgaaa
     2761 taatgaaatc tgtgtcagta ttgactactt gatttcttct ccaattttcc aatactacta
     2821 ttgtcacatc atctaactca ataagacgtt ctcctgctgg cgtttttgtt tgatctttac
     2881 gataccaatt tccattttca tccttttcca aggttgtatg aactttcaaa aatttttgtt
     2941 cgaaatcgat atcttcccat cataatgata aaccttcact tacacgaaca cctgtcatat
     3001 aatatagcca aatagttgta aatctttgta gctcttcata atcttgcaaa tcaaacgatt
     3061 ttataaaatt ctgaaactca atataagtcc aaaatggagt atcgggatgt ttccctttag
     3121 gattatccaa agccttacac ggcacttttg aaatatagtc taacctttca gcatacccca
     3181 tgcaagcttt gaatcgcgac cataaattct ttgcataatt ttcaataata tgtaaactaa
     3241 aagattcaca atctcaagct gttattccct ttaaaagttt agaaccaaaa tactgaataa
     3301 ataacttatg atgtggtaag gctatttcat aagttacagc ctgaaccttt tgcttatatg
     3361 ctcttaggta aatagtattc atatagtttt caaacgatat attgtcattt tcaatactaa
     3421 gattattact aaacccatac ctaatccgaa ccagttcatc atacgcttct ttcattgtgc
     3481 caaatggctc tccatactaa ttctttctgg ctttcttttg aactcgttta cctgttactg
     3541 gatctacacc aagatcagct tgataaaata tcttaccatt cttatcgcaa taaacacctt
     3601 tgtacttttt ttgatttttt atagtcatct ttaattctca ctttctaaac tttcttcaga
     3661 gagtagaata cctattaact cttctacaat atctttagga gcaataccaa gtcttctatt
     3721 gtcaaagggc gatttactta attgtaccgc attttggtca atttcgcgag cttcttcaaa
     3781 cttttttata gctattcttt ttgcttctct aataatatct cttgcagtat gttcgagaaa
     3841 accaatattc atcaagcttt ttatatgtta ctattttcac acctccactc ctttcaaaaa
     3901 aattgcaacc tccgtgggta tgactggtta aatcactcat gggaatctca cccactggcc
     3961 ttttacgctg gcagcctgaa cgttcagaag tatcattgtc cctatttgtg gtcatggcag
     4021 atagagaacc actctatttt atagctaata attatcgctc ctatcacggc gtatctcact
     4081 aaattgctcg acaaacaaga aagtcccacc tcggtttatt cagttgtcaa tgaacaaatc
     4141 aaggcgagac ttttatttat tatgaaaacg atacggataa tctgtttgcc ctaataaata
     4201 atcaatactg acgttataga gtttagaaaa ctgcacaaga acatctgcca ttaaggcaac
     4261 ctctcctctc tcaatttttg cataatttgc atgagagaag gaaagtaaat ttgcaacaaa
     4321 cttttgagtg aaatcgttat cttctctcaa attcctaata cgcggataca tctaacattc
     4381 tcctaatatc agtataaagg agatttgatt aaaaaatgtt taattgtcct atttggacaa
     4441 gactattaaa tataagctag taatccattg aaattttata gtcatttttt agttaaacaa
     4501 gttagatagt atttataact taaaaaatca tatttattgc taggtaaaca aaatagatgt
     4561 atttaactac ttgtcaaaca gtcttgattc caacttttaa atattcgtat atgaagtaaa
     4621 gctactgtgc atgaaacaaa aaatataaca ataagtggaa caattgccat tttctgttgg
     4681 ttatatagag tatctgatat tcttaaaagc catgcccatg gaataagaaa ccatacctta
     4741 tcaccaaggc tcgtttcaca aaatgctgca gcaataacac ctaccattga aatacctaat
     4801 gccacacctg tactaaatat atagctaata atatgattga tcagataggt tacataggca
     4861 aaaatgatat aaattaaact attcaaaata agtagtctga gagatacatt tataccaata
     4921 gttttcaata aaagaataaa tatagtaatt gaaataatta atactataca atagcataac
     4981 catagaaata ctaattctac aataaatggc ttataattcg actgtgagta acccaataaa
     5041 tatttatatt ttccagatga ttcttctaaa ttttttagtt gaatgcacac aatacttgca
     5101 acaaaaggaa gaagtacacc tactatttga aagtaggaga ataatacagt cctgctttgt
     5161 aaacctccac gttgactcca atagaaaaat agaagtaagg gaaataatag tgcagctaaa
     5221 atatgtatta gtaaatatga agtatttttg atttttaaaa aattagaaca taaatatgat
     5281 ttcatgacta tttcacctgt tttttgaaag agatagcaaa tagattggtc agtaagatga
     5341 aaaaagcaat tcctaggaac aatgatataa aaatctgtga gtagtctgga acaaactccg
     5401 ctccaaaagg tatactattt attctcattc ttgtcattgg ctcaagcaac ttcaacgacc
     5461 aatcataagg gaacaaatac catttattta agagagaaaa acttgtacca ataattagtg
     5521 gagatataat attaataatc atagcaaaat acacattaat ttttcttgat agccataaaa
     5581 taaacgggat ttgccatagt gtagtcaacc aaattaacaa gatagttaat agtatcctac
     5641 ctatactaat aagacttgga gtaggtagga caaaactaat tacaataagc attaagctta
     5701 aaatcatact aattattaga tttataagag ctatcaatag aactctggca tattctacct
     5761 tttttaaatc aacatccctt gataaaaaaa cagaatactt attagaatct aattctaatt
     5821 tatttaacaa accagaattt acagcaataa taacattaat ccaaataact ccccactgat
     5881 ttactgttgt ttccgcagtt aaacgtagga tattttcacc acctaatgct gaaaacaata
     5941 aagataacat cacacaaata atagggaaaa taagaatcaa cttgctaata ctcgtatttt
     6001 ttatttttaa taaatttgat aaaaaaattt gtaccatagc ccctacctcc tcttttcaaa
     6061 taaaatttga ttattgaata actcttcaag attctcagag ccgtcatatt ctttttccag
     6121 aataatcttt ccttcataga taaatccaat aaaatcagct agatgttcaa cttctgacag
     6181 tatatggctt gaaatcataa ttgtcattcc ttctgattta aatgactcta taatttccct
     6241 taattcttga attccaattg ggtccaagcc attagtaggt tcgtctaata taagtagttt
     6301 aggtgaattt attaaagaga tagctatacc taaccgttgc ttcattccaa gagaaaattg
     6361 ttttactggt ctcttgtcaa tatttcctag accaacttta tttaataatg gaagtatagt
     6421 gctttctgaa acaccaagca ttgttgtaac taccttcata ttatcgtaag cactcaaatt
     6481 tttataaagt ggtggttctt caataatact cccgattttt cgcagatccc gacgtgacca
     6541 ttctctacct tcaaaaataa tagaaccact tgtcttacta actaagcctg tcaaaatttt
     6601 cataatagtt gattttccag caccatttgg accgattaaa gcatacactt ttcccttagg
     6661 aatattcata ttgacattat tcaatatggt tctcttacca taagattttt ttagattttg
     6721 tattttcaac atattagtta ctcccattct atatattgct gagcgtttag tgtatagtaa
     6781 gcaaaacaat acactttctc ttcaaactct ctatcaatcc cgtttaatcg attcattgat
     6841 agatgtaaaa aacttcctac taaattgaat ttagtattat agatatcatt tgtaccacca
     6901 aatcgattat actctttcat ataagacatg agatatctat ccatttcttg gaaaaaatca
     6961 tatctatcaa gagctgaggt gactccattc aaaatcttca tatactcaat aggtaaatca
     7021 attgg
//
LOCUS       DAUCLR010000090         6987 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.091, whole genome shotgun sequence.
ACCESSION   DAUCLR010000090 DAUCLR010000000
VERSION     DAUCLR010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6987
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.091"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..208
                     /locus_tag="VGT87_001571"
     CDS             <1..208
                     /locus_tag="VGT87_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1273 family protein"
                     /protein_id="HEQ3814407.1"
                     /translation="KYYFPNYEQPPQFSQYYQFLLEKTEGAYVFYDTENETNLKYFLK
                     KAKDMPYYQLLLLTFDRLNDMSQS"
     gene            327..653
                     /gene="gpsB"
                     /locus_tag="VGT87_001572"
     CDS             327..653
                     /gene="gpsB"
                     /locus_tag="VGT87_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEQ3814408.1"
                     /translation="MTSIIYSPKDIFEQEFKTSMRGFDKKEVDEFLDNVIKDYENFNA
                     QIEALKAENEALKKAKFQARNTVSATVQQPVPQPTRVAQSATNFDILKRISKLEKEVF
                     GKQIIE"
     gene            667..1035
                     /gene="rnpB"
                     /locus_tag="VGT87_001573"
     ncRNA           667..1035
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VGT87_001573"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            1098..2252
                     /locus_tag="VGT87_001574"
     CDS             1098..2252
                     /locus_tag="VGT87_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEQ3814409.1"
                     /translation="MKETFRLVATAAAGLEAVVGKEVRALGFDCQVENGKVYFEGDVE
                     AIVKTNLWLRAADRIKIIVGQFPARTFEELFQGVFALDWGNYLPLGAKFPISKAKCVK
                     SKLHNEPSVQAITKKAVVKKLQKHFHRPEGVPLQEVGSTFNIEVSILKDQATIMIDTT
                     GSSLFKRGYRVQKGGAPIKENMAAAILALSNWFPDKPLVDPTCGSGTFCIEAALIGMN
                     IAPGFNRSFAFEEWSWVDKDMVQQVRDDAEQEANYEIELDISGFDIDDRMIEIAKSNA
                     EEAGLSDVITFKQMRLQDFRTDKVNGVVISNPPYGERLLDDKAVDILYNEMGQTFAPL
                     KTWSKFILTSDELFELKYGQKADKKRKLYNGTLKVDLYQFYGERVKRHLI"
     gene            2266..3735
                     /locus_tag="VGT87_001575"
     CDS             2266..3735
                     /locus_tag="VGT87_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814410.1"
                     /translation="MSEESKEVEVTKESQTLGLNEAKSMTIGEAVRKQSEIKAGVTKD
                     DSILDKYIKQHRDEVSSQKFDAKYTELDTASLDNFIKKQREALSKAGLVDDEPVSAES
                     AEQDSTLVEEVAEDLAPMETTAVVTGIPVEATVPVLDLDPSERVIPEPQMTKEEPKRD
                     QFLSEDSHHPAKQNTKKGWLIALFLLLLGILAVVFGWNHFLRQDSGKTTQTASKQTKT
                     SLQTDSAKKAMRLKAAAKAFEKQYGTFYTDATKSKLKNSAFATLPDLEAALKALEGSA
                     YYDKAKAKVDSLKKAIAAITAVNGKFVSDAVVDGEKVSAEVKADANFDDLSSATLTTG
                     NANLDAVLQASITEGRQQLASKAEAAKAANEQEAQDQAAQGQSTSVAPSGYGLTSYDP
                     ASLQRHLSRVPYNQDVIADRANPSWAFNPGVLEKIVATSQARGYISGNQYILEPVNII
                     NGNGYYNMFKPDGTYLFSINCKTGYFVGNGKGYADALDY"
     gene            complement(3880..4362)
                     /locus_tag="VGT87_001576"
     CDS             complement(3880..4362)
                     /locus_tag="VGT87_001576"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEQ3814411.1"
                     /translation="MTKEVIVESFELDHTIVKAPYVRLISEEFGPKGDRITNFDVRLV
                     QPNQNSIETAGLHTIEHLLAKLIRQRIDGMIDCSPFGCRTGFHLIMWGKHSSTDIAKV
                     IKSSLEEIATGITWEDVPGTTLESCGNYKDHSLFAAKEWAQLIIDQGISDDPFSRHVI
                     "
     gene            complement(4436..5767)
                     /locus_tag="VGT87_001577"
     CDS             complement(4436..5767)
                     /locus_tag="VGT87_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEQ3814412.1"
                     /translation="MEIIGSLGVLVGVIVIIYLSVKEVNIIIAAPLATSLVIWFNQMD
                     PTTTLLGKEPNQFMGALSTYILNYFAIFLLGSILAKLMETSGATTSIADYILKKVGHD
                     SPYKVLVAIFLISAILTYGGISLFVVMFAVLPLARSLFKKMDLAWNLIQVPLWLGIAT
                     FTMTILPGTPAIQNVIPIQYLDTSLTAAAIPSIVGSIGCVTFGLFYMKYCLAKSMARG
                     ETYTTYAFDNEIQVKTKNLPHFLASILPLLLLIIIALTGSLFGNDFFKKNIIFIALLA
                     AILTASWLFRQFIPNKIAVFNLGASSSIAPIFATASAVAFGAVVMIAPGFTFFSDLIL
                     NIPGNPLISLAVLTSSMSAITGSSSGALGIVMPNFAQYYLDQGLNPEMIHRVATIASN
                     IFTIVPQSGVFLTFLALTGLNHKNAFKETFITVSVSTFIAQVIMIAFDLFS"
     gene            complement(5838..6617)
                     /locus_tag="VGT87_001578"
     CDS             complement(5838..6617)
                     /locus_tag="VGT87_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEQ3814413.1"
                     /translation="MTHTKEVAFITGAASGIGKQIGETLLKEGKTVVFSDINQEKLDQ
                     VVADYTKEGYDAFSVVCDVTKEEAINAAIDTVVEKYGRIDILVNNAGLQHVAMIEDFP
                     TEKFEFMIKIMLTAPFIAIKRAFPTMKAQKHGRIINMASINGVIGFAGKSAYNSAKHG
                     LIGLTKVTALEAADSGITVNAICPGYVDTPLVRGQFEDLSKTRGIPLENVLEEVLYPL
                     VPQKRLIDVQEIADYVSFLASDKAKGVTGQACILDGGYTAQ"
     gene            complement(6650..>6987)
                     /locus_tag="VGT87_001579"
     CDS             complement(6650..>6987)
                     /locus_tag="VGT87_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinyl-CoA--3-ketoacid-CoA transferase"
                     /protein_id="HEQ3814414.1"
                     /translation="VLGALQVDKEASIANYLIPGKMVPGMGGAMDLLVGAKKVIVAME
                     HTNKGKAKILDKCTLPLTAQNVVNLIITEMGVFEYQDEGLCALEINQDYTFEDVQNVT
                     EVTLIDKTN"
BASE COUNT         2307 a         1232 c         1470 g         1978 t
ORIGIN      
        1 aaaatattac tttccaaatt acgaacaacc gccgcaattt agtcaatatt atcagttttt
       61 attagaaaag acagaagggg cttatgtttt ttatgacaca gaaaacgaga ccaacttaaa
      121 atattttctc aagaaagcaa aagatatgcc gtattatcag ttgctattgc taacctttga
      181 tcggctaaat gatatgtcac aaagctagca ttgtattttt aggggtattg gtatttaaat
      241 acttgctttt tgaactattt ttctgtatca ttaataatac tgaaatataa ttttggtaaa
      301 aagattgacc gttcggagag taaagaatga caagtattat ttatagtcct aaagatattt
      361 ttgagcaaga atttaagact agtatgcgag ggtttgataa gaaagaagtt gatgaatttc
      421 ttgataatgt aattaaagat tatgaaaatt tcaatgctca aattgaagct ttaaaagctg
      481 agaacgaagc cttaaaaaag gcgaagtttc aagctagaaa tacagtttca gcgactgttc
      541 aacaaccagt accgcaaccg actcgtgttg ctcaatcagc tactaatttt gacatcttga
      601 aacgtattag taaattggaa aaagaggttt ttggtaaaca aattattgag taatgaaaac
      661 tgtgcaattt ttggataatc gcgtagtatt ttaatactat gaggaaagtc catgctagca
      721 ctggctgtga tgccagtagt gattgtgcta ggcgaacaca taagcctagg gatgtgcata
      781 cacattacgg cgaaggaaat ggctaagtcg tttgatatgc caaagtactt ctgaaagtgc
      841 cacagtgacg tagtttttat ggaaacgtaa aaaatggaac gcggtaaacc cctcaagcta
      901 gcaacccaaa ctttggtagg ggcatgggat agttggaaac gaacaagcta tcctgactgt
      961 caacagacgg tagacagatg attatcgaag gaaataattc ctagttattt ccggaacaaa
     1021 acatggctta tagaaaattg cataataggt taagctagca cttgctagct tttatttgat
     1081 ttaaaagaaa gagatatatg aaagaaacat ttagattagt cgcaactgca gccgcaggtt
     1141 tagaagctgt cgttggtaaa gaagtcagag cactaggttt tgattgtcag gttgaaaatg
     1201 ggaaagttta ttttgaaggt gatgtagaag cgattgttaa aacaaatctt tggttacgtg
     1261 cagcagatcg tatcaaaatt atcgtaggac aatttcctgc tagaaccttt gaagaattat
     1321 tccaaggtgt ctttgccttg gattggggaa attatttacc gctaggagct aagtttccaa
     1381 tctcaaaagc caagtgtgtc aagtcaaagc tacacaatga gccaagtgtt caggcgatta
     1441 ccaaaaaagc tgttgttaag aaactgcaaa aacatttcca caggccagaa ggagttcctc
     1501 ttcaagaagt tgggtcaacg tttaatattg aggtatctat tttaaaagac caagccacta
     1561 ttatgattga cacgacagga tctagcctat ttaaacgagg gtaccgcgtt caaaagggtg
     1621 gggctcctat taaagaaaat atggcagctg ctatcttggc cttatctaac tggtttccag
     1681 ataaacctct ggttgatccc acctgtggat cgggaacgtt ttgcatcgag gcagccctga
     1741 ttgggatgaa tattgctcca ggatttaacc gttcgtttgc ttttgaagaa tggagctggg
     1801 tagataaaga catggtgcaa caagttcgtg atgatgccga acaagaagct aattatgaaa
     1861 tcgaattaga tatttctggc tttgatatcg atgaccgcat gattgagatt gctaaaagta
     1921 atgctgaaga agctggttta tctgatgtga ttacctttaa gcaaatgcgt ctgcaagact
     1981 ttcgaacaga caaggttaat ggtgtagtga tatcaaatcc tccttatgga gaacggttgc
     2041 ttgatgacaa agcggttgac atcttgtata atgaaatggg acaaaccttt gcaccattga
     2101 aaacatggag caaatttatc cttacaagtg atgaactttt tgaactaaaa tacgggcaaa
     2161 aagctgataa aaaacgtaaa ctttataacg gcaccctaaa agttgatttg taccaatttt
     2221 atggggaaag ggtaaagcga catctgatat agagaggtag taagcgtgtc agaagaaagc
     2281 aaagaagtcg aagtaacaaa agaatcgcaa acattggggc ttaatgaagc caagtcaatg
     2341 acaattggtg aagctgtacg caaacagtca gagataaaag caggtgttac taaggatgat
     2401 agtattttag ataagtacat taagcaacac cgtgacgaag tttcttctca aaagtttgat
     2461 gctaagtata cagagcttga tacagctagt ttagataact ttatcaaaaa acaaagagaa
     2521 gcattgagta aagccggtct tgttgatgac gaacctgtgt ctgcggagtc tgctgagcaa
     2581 gatagcactt tggttgaaga agtagctgaa gacttagctc caatggagac tactgcggtg
     2641 gtaactggga ttccggtgga agcgacagtc cctgtgttag atctagatcc atcagagagg
     2701 gtcattccag aaccacagat gaccaaggaa gaaccaaaaa gagatcagtt tctttctgaa
     2761 gatagccacc atccagccaa acaaaacacg aaaaaaggtt ggttaatagc tttatttctg
     2821 ctattattgg gcattcttgc agttgtattt ggttggaacc acttcttgag acaagacagt
     2881 ggtaaaacaa ctcaaacagc aagtaaacag acaaagacct ctttgcagac cgacagtgct
     2941 aaaaaagcaa tgcgcttaaa ggctgctgct aaagctttcg aaaaacaata tggtactttt
     3001 tatacagatg ccacaaagag caaattaaaa aatagtgcct ttgctacttt gccagattta
     3061 gaagctgcct tgaaagcttt ggaaggctct gcttactatg ataaggcgaa agcaaaagtc
     3121 gattccctta agaaagctat tgctgctatt acagcggtta acggtaaatt tgtttccgat
     3181 gctgttgtag atggtgaaaa ggtttcggct gaagtcaagg ctgatgctaa ttttgatgat
     3241 ttatcaagtg caaccttgac aacaggaaat gcaaatcttg atgctgtctt gcaggcaagc
     3301 atcacagaag gtcgtcagca actagccagc aaggctgagg ccgctaaagc agctaatgag
     3361 caagaagccc aggatcaagc agctcaagga caaagtacat cagtagcacc atctggctat
     3421 ggcttgacaa gttatgatcc tgcaagctta caacggcatt tatcacgtgt accttacaat
     3481 caagatgtta ttgctgacag agctaaccca tcttgggcat ttaatccagg agttcttgaa
     3541 aaaattgtag caacctcaca agctagaggt tatatttcag gcaaccaata tattttagag
     3601 ccggttaata ttattaatgg caatggttac tataatatgt ttaagcctga tggcacttat
     3661 ctgttctcta ttaattgtaa gacgggttat tttgtaggaa atggcaaagg ttatgctgat
     3721 gctttagatt actgagaaaa tcaaaaaaac tgagataagg caacttgtct cagttttttg
     3781 attaataaag tatgttgtct taaaatctgc agatagactt gttttttgta acaagtcaaa
     3841 taggtcagta ggattgttat gtttgacaac acactagtat cagatgacat ggcgactaaa
     3901 aggatcgtct gaaatccctt gatcaataat caattgagcc cattctttgg cggcaaaaag
     3961 gctatgatcc ttatagtttc cacaggattc aagagttgtt ccaggaacat cttcccaagt
     4021 aatcccagtt gcaatttctt ctaggctaga tttaatcacc ttggcaatat cagtagaact
     4081 gtgttttccc cacatgataa ggtgaaaacc tgttcgacag ccaaaaggag agcaatcaat
     4141 catcccatca atgcgttggc ggatgagctt ggcaagtaag tgttcaatgg tatgcaaacc
     4201 ggctgtttca atagaatttt ggttaggctg cactaggcga acatcaaaat ttgtaatacg
     4261 atcgcccttg ggtccaaatt cttcagaaat aagacgaaca taaggggcct ttacaatagt
     4321 atgatctagc tcaaaacttt cgacgataac ttcttttgtc atttcaatct cctttgtttt
     4381 acactttatc ataacacaaa tagtgagaaa gtggctactt gtggaggaga aaaggctaag
     4441 aaaacagatc aaaggctatc ataatgacct gtgcgataaa agtacttaca gaaacagtaa
     4501 taaaggtttc tttaaaagca tttttgtggt taagtcctgt caatgctaag aaggtgagaa
     4561 agacaccact ttggggcaca atggtaaaga tattcgaagc aatagtggca acacggtgaa
     4621 tcatttcggg attaaggcct tgatctaaat aatactgggc aaagttaggc atgacaattc
     4681 ctagagcacc tgaagaagaa ccggtaatag ctgacataga ggaagtcaac acggctaaac
     4741 taataagagg attaccggga atattcaaaa tgagatctga gaagaatgtg aaacctgggg
     4801 caatcatcac aacggctccg aaggcaactg cagatgctgt cgcaaaaata ggtgcaatag
     4861 agctgctagc tcctaaatta aagacagcaa tcttattagg aataaactgt ctaaagagcc
     4921 agcttgcggt gagaatggca gctaaaagtg ctatgaaaat aatgttcttc ttaaaaaaat
     4981 cgtttccgaa aaggctaccg gttagggcga taataatgag caacaataaa ggtaagatac
     5041 tggcaaggaa atgtgggaga ttttttgttt tcacttgtat ctcattgtca aaagcataag
     5101 tggtataagt ttcacccctt gccatacttt ttgctagaca atatttcata taaaagagcc
     5161 caaaagtcac gcagccaatt gagccaacga tactcggaat ggcggcagca gttagtgagg
     5221 tgtccaagta ttgaatagga ataacatttt gaatggcagg tgttccgggt aaaatcgtca
     5281 tggtaaaagt cgcaataccg agccaaagag gaacttgaat taagttccaa gccaagtcca
     5341 tttttttgaa tagacttcgt gcaagcggca agacagcaaa catgacaaca aagaggctga
     5401 ttcccccgta agttaatata gcactgatca gaaaaatagc aacgagtact ttataaggac
     5461 tatcatgacc aacttttttt aaaatataat ctgcaataga agtggttgca ccgcttgttt
     5521 ccattaattt ggctaagata gaacctaata aaaagatagc aaaataattg agaatataag
     5581 tggagagtgc ccccataaat tggttgggtt cttttccaag taaggttgtg gttggatcca
     5641 tttgattaaa ccaaatgacc aagctggtag caagaggcgc agcgatgata atgttaactt
     5701 ctttgacaga caggtaaata ataacaataa cacctaccaa gacccctaga gaaccaataa
     5761 tttccatagt tttatgacct ccttattaga aaataaagag acagatcaca agatacagcc
     5821 tctttatctc tccaaattta ttgagcagtg tagccaccgt ctaagataca ggcttgacct
     5881 gtaacacctt ttgccttatc actggcaagg aaagacacat agtctgcaat ttcttgaacg
     5941 tcaatgaggc gtttttgagg aacaagtggg tatagcactt cttcaagaac attttcaagg
     6001 ggaatacctc ttgttttgga aaggtcttca aactggccac gaaccagtgg tgtgtcaaca
     6061 tatccaggac aaatggcatt gaccgtaatg cctgaatcag cagcttctaa ggcagttact
     6121 ttggtcagac cgatcaagcc gtgcttagct gaattgtagg cggatttgcc agcaaaacca
     6181 atgacaccat tgatagaagc catattaata atacgaccgt gtttttgagc tttcattgta
     6241 ggaaaagcac gtttaatggc aataaatggt gctgtcaaca tgattttaat catgaattca
     6301 aatttttcag ttggaaaatc ttcaatcatg gcaacatgtt gaaggcctgc gttgttaacc
     6361 aaaatatcaa tacgaccata tttttcaaca accgtatcaa tagcagcatt gatggcttct
     6421 tctttggtga catcgcacac aacactaaaa gcgtcatagc cttctttagt atagtcagca
     6481 acaacctgat ccagtttttc ttgattaatg tcagaaaaaa caaccgtttt accttctttt
     6541 aataaggttt ccccgatttg ttttccaatt ccgcttgcag caccagtgat aaatgcaact
     6601 tctttagtat gtgtcatgat actatcctcg tctttctttt ggttagtctt tagtttgttt
     6661 tatcaattag ggtaacttcg gtaacgtttt gcacgtcttc aaaggtgtaa tcttggttaa
     6721 tttcaagggc gcatagacct tcatcttgat attcaaagac tcccatttca gtaatgatta
     6781 ggttaacaac attttgagca gtcagaggaa gggtgcattt gtctaaaatt tttgctttgc
     6841 ctttattggt atgctccatt gcaacaatga cttttttagc acctaccaat aagtccatgg
     6901 cccctcccat cccaggaacc atttttccag gaatcaggta attagcaatg ctagcttctt
     6961 tatcaacttg taaggcacct aagacgg
//
LOCUS       DAUCLR010000091         6908 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.092, whole genome shotgun sequence.
ACCESSION   DAUCLR010000091 DAUCLR010000000
VERSION     DAUCLR010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6908
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.092"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            24..575
                     /locus_tag="VGT87_001580"
     CDS             24..575
                     /locus_tag="VGT87_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEQ3814415.1"
                     /translation="MSCCFFVVLFILGIVKKKRRLKMSIETRAAFEKVKPIILKLKRH
                     YYIQLWDRDDWLQEGHIILLQLLERYPELIEEEERLYRYFKTKFSSYLKDLLRRQESQ
                     KRQFHKLAYEEIGEVAHAIPSRGLWLDDYVAYQEVIASLENQLNSPERMQFQALIRGE
                     RFKGRRALLRKISPYFKEFAQQL"
     gene            1156..1683
                     /locus_tag="VGT87_001581"
     CDS             1156..1683
                     /locus_tag="VGT87_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEQ3814416.1"
                     /translation="MSIDDYRPTYMVEAIYDLRANDLLRHGITAVLVDLDNTLIAWNN
                     PDGTPEVRAWLDEMTIADISVVVVSNNKHSRVERAVSRFGVDFISRALKPFAYGIEKA
                     IARYGFDRNEVIMVGDQLMTDIRASHRAGIKSVLVKPLVASDAWNTKINRWRERRVMA
                     KLEEKYGKLSYQKGI"
     gene            1683..2801
                     /gene="yqeH"
                     /locus_tag="VGT87_001582"
     CDS             1683..2801
                     /gene="yqeH"
                     /locus_tag="VGT87_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEQ3814417.1"
                     /translation="MEELFCIGCGIQIQTEDKEKAGFTPAAALKKGMETEELYCQRCF
                     RLRHYNEITDVHITDDEFLRLLHEVGDSDALVVNVIDIFDFNGSIIPGLSRFISGNDV
                     LLVGNKKDILPKSVKDGKVTQWLTERAHEEGLRPLDVMLTSAQNKYAIKDLIGRINEL
                     RNGRDVYVVGVTNVGKSTLINAIIQEITGNKDVITTSRFPGTTLDKIEIPLDDGTFIF
                     DTPGIIHRHQMAHYLSPKELKIVSPKKEIKPKTYQLNPEQTLFLGGLARFDFINGERQ
                     GFTAFFDNQLELHRTKLAGADAFYDKHVGTLLTPPDKKELTAFPKLVRHEFTIDQKMD
                     IVFSGLGWIRVNGQKDSKAIVAAWAPEGVAVIVRKAII"
     gene            2826..3134
                     /gene="yhbY"
                     /locus_tag="VGT87_001583"
     CDS             2826..3134
                     /gene="yhbY"
                     /locus_tag="VGT87_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEQ3814418.1"
                     /translation="MLTSKQRAFLKSEAHSLKPIVQIGKNGLNDHIKTSIRQALDARE
                     LIKVTLLQNTDEDIHEVAEILEEEIGCDTVLKIGRILILYKVSAKKENRKLSPKVKAI
                     "
     gene            3203..3835
                     /locus_tag="VGT87_001584"
     CDS             3203..3835
                     /locus_tag="VGT87_001584"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEQ3814419.1"
                     /translation="MALELLTPFTKVELEEEKKESNRKQIGILGGNFNPIHNAHLVVA
                     DQVRQQLGLDQVLLMPECKPPHVDAKETIDEKHRLRMLELAIEDVEGLAIETCELERQ
                     GISYTYDTMLYLTEQHPDVDYYFIIGADMVDYLPKWHRIDELVKLVQFVGVQRPKYKA
                     GTSYPVIWVDLPLMDISSSMIRDFIKKGRQPNYLLPKRVLDYITQEGLYQ"
     gene            3832..4425
                     /gene="yqeK"
                     /locus_tag="VGT87_001585"
     CDS             3832..4425
                     /gene="yqeK"
                     /locus_tag="VGT87_001585"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEQ3814420.1"
                     /translation="MTYEDYLPYSRTELLAKIAEQMSPKRFKHVLGVEKAALSLAECY
                     GCNPDKAGLAALLHDYAKECPDQVFLDLIDKYQLSPELAKWNNNVWHGMVGIYKIQED
                     LGLKDKDILRAIEIHTVGAAEMTLLDKVLYVADYIEEGRIFPLVDDARKIAKLDLNQA
                     VAYETVNTVAYLASKAQPIFPQTLDTYNAFCSYLKEY"
     gene            4449..4802
                     /gene="rsfS"
                     /locus_tag="VGT87_001586"
     CDS             4449..4802
                     /gene="rsfS"
                     /locus_tag="VGT87_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEQ3814421.1"
                     /translation="MKKEELLKIVVEATDEKRAKDILALDLEGLTSLTDYFVIASATN
                     SRQLEAIADNIREKVKEAGGDASHVEGNSQAGWVLLDLTDVVVHLFLEDERYHYNLEK
                     LWHEAPAVALDAYLA"
     gene            4850..5593
                     /locus_tag="VGT87_001587"
     CDS             4850..5593
                     /locus_tag="VGT87_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEQ3814422.1"
                     /translation="MEHNYEKFASVYDAVMDDSLYDLWTDFSLRHLPKSKSRNRLLEL
                     ACGTGIQSVRFAQAGFDVTGLDLSQDMLAIAKKRAQSAKKKIDFIQGNMLDLSQVGQF
                     DFVTCYSDSICYMQDEVDVGDVFKEVYDVLANDGIFIFDVHSTYQTDECFPGYSYHEN
                     ADDFAMVWDTYADEVPHSVVHELTFFIQEDDGRFSRFDEVHEERTYELLTYDILLEQA
                     GFKSFKVYADFEDKEPTETSKRWFFVAYK"
     gene            5814..>6908
                     /locus_tag="VGT87_001588"
     CDS             5814..>6908
                     /locus_tag="VGT87_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEQ3814423.1"
                     /translation="MTVTGIIAEFNPFHNGHKYLLETAEGLKIIAMSGNFMQRGEPAL
                     IDKWIRSEMALKNGADIVVELPFFVSVQSADYFAQGAIDILCQLGIQQLAFGTEDVID
                     YQKLIKVYEKKSEQMTAYLSTLEDTLSYPQKTQKMWEIFAGVKFSGQTPNHILGLSYA
                     KASAGKHIQLCPIKRQGAAYHSKDKNHLLASASAIRQHLNDWDFISHSVPNAGLLINN
                     PHMSWDHYFSFLKYQILNHSDLTSIFQVNDELASRIKKAIKVSQNIDHLVDTVATKRY
                     TKARVRRILTYILVNAKEPTLPKGIHILGFTSKGQAHLKKLKKSRPLITRIGAETWDE
                     MTQKADSIYQLGHQDIPEQSFGRIPIIIKNE"
BASE COUNT         2188 a         1048 c         1503 g         2169 t
ORIGIN      
        1 atgaaaaaaa caacaggaca ctaatgtcct gttgtttttt tgtggttctc tttatactag
       61 ggatagttaa aaagaaaagg agactcaaaa tgtcgataga gacaagagca gcgtttgaaa
      121 aagttaagcc cattattttg aaattaaagc gacactatta tattcaattg tgggatagag
      181 atgactggtt acaagaagga catattattt tattacagtt actagaaagg tacccagagt
      241 taattgagga agaagagcgt ttatatcgct attttaaaac aaaattttca tcttatttga
      301 aagatttatt acgccgtcaa gaaagtcaaa agcgtcagtt ccataagtta gcatatgaag
      361 agatagggga ggttgcacat gccattccat cgagagggtt atggctagac gactatgtgg
      421 cttatcaaga ggtaatagct agcttagaga accaattaaa ttcgccagag cgtatgcagt
      481 ttcaagcact tatcaggggc gaacgtttca agggaagacg tgctctactt aggaagatca
      541 gtccctattt taaggaattt gcacagcagt tgtaagcaag acctataagg aattgggggt
      601 aaatctatga aaaaagtgta atcatttttt gatttacagt tattatcatg tgttatctgg
      661 cgactgtcct gtttacaagt aagttcacaa gtatgggata aaatagtata aactatagta
      721 aatactgaat agtcatagaa aaatatcggc ataggtcaga agagctgata tgtttaacac
      781 tttttgcgga tatctactgt atttaaatgt atttgatatc cttgtcaagg atatacgtct
      841 aaacaagttc aaaacgaaat catttgtgat tatcttatca ggagttgact cttgataaga
      901 tttttgtata tcagtcctgt ggtacgttta gatagcttgg cagtgaattt atagagtgtc
      961 aaacaacgag tgttttgtgt tacactaacc attagaagag tctatttttg agcatatcaa
     1021 ggtgttatct tttcgagttg aggtatcata tctagttgta atgtcaaaca tatctagtat
     1081 atttaactat ggttttaaat gctatttcaa aaaaaatatg ataaaataga aagtactaat
     1141 tattttggag atgacatgag tatcgatgat tataggccga cctatatggt agaggctatt
     1201 tatgatttaa gagcaaatga tttgttgcgt catggtatta cggctgtttt agtggattta
     1261 gataatacct taattgcatg gaataatcct gatggcacac cagaggttcg cgcttggctt
     1321 gatgaaatga ctatcgcgga tattagcgtg gttgttgttt caaataataa acattctcgt
     1381 gttgagcgtg ccgtttcacg ctttggtgtt gactttatta gccgggcttt gaagccattt
     1441 gcttatggta ttgaaaaagc cattgctcgt tatggatttg atcgcaacga ggttatcatg
     1501 gttggagatc agctaatgac tgatatcaga gctagtcata gagctggaat aaaatcagtt
     1561 ttagtcaaac cacttgttgc ttctgatgct tggaatacta aaattaaccg ctggcgtgaa
     1621 cgccgtgtga tggcaaaatt agaagagaaa tatggtaaat tatcatatca aaaaggaatt
     1681 taatggaaga attattttgt ataggctgtg gtatccagat tcaaactgaa gataaagaaa
     1741 aagctggttt tacgccagct gctgctttga aaaagggtat ggaaacagaa gaactctatt
     1801 gtcagcgctg ctttagattg aggcattata atgaaattac agatgtccat attaccgatg
     1861 atgaattttt aagacttctt cacgaagtgg gtgatagtga tgctcttgtg gttaatgtga
     1921 ttgatatttt tgactttaat ggttctatta ttccaggtct atctcgcttt atttctggta
     1981 atgatgtctt actggttggg aataaaaaag atattttgcc aaagtctgtt aaggatggta
     2041 aagttactca atggttgacg gaacgtgcac acgaagaagg tcttcgccca ttagacgtta
     2101 tgttaacgag cgctcaaaat aaatatgcga ttaaagactt gattgggcgt atcaatgagt
     2161 tacgtaatgg tcgagatgtt tatgttgtgg gggtcactaa tgttggtaaa tcaaccttga
     2221 ttaatgcgat tatccaagag attactggta acaaggatgt catcacgacg tctcgttttc
     2281 ctgggacaac tcttgacaaa attgaaattc ctttggatga tgggacgttc atttttgata
     2341 cgccaggaat tattcacagg caccagatgg ctcattattt atcccctaaa gagctaaaaa
     2401 tagtgagtcc taagaaggaa atcaagccta agacgtatca attaaatcca gaacaaactc
     2461 tctttttagg tggtctggct cgctttgact ttatcaacgg tgagcgtcaa ggatttacag
     2521 ctttttttga caatcaacta gaattacatc gtacaaaatt agctggagca gatgcttttt
     2581 atgataagca tgttggaact ttgttaaccc cgccagataa gaaagagttg actgcatttc
     2641 ctaaattagt tcgtcatgaa tttacaattg atcagaaaat ggatattgtt ttttctggtc
     2701 taggatggat tcgtgttaat ggtcaaaaag actcaaaagc gatagttgct gcatgggctc
     2761 ctgaaggggt cgctgtcatt gttcgtaaag cgattattta aaaataaaaa agaagttgga
     2821 aaaaaatgct tacaagtaaa caaagagctt tcttaaaatc tgaggctcat agcttaaaac
     2881 cgattgtaca aattggcaaa aatggattaa atgaccacat taagactagt attcgtcaag
     2941 ctttggatgc tagagaattg ataaaggtca ctttgttgca aaatactgat gaagatattc
     3001 atgaagtagc agagatttta gaagaagaaa ttgggtgtga tacggttttg aaaattggtc
     3061 gtatcttgat tctctataag gtttctgcta agaaagaaaa ccgcaagttg tcccctaaag
     3121 ttaaagcaat ctagtccttt tatttgtata gggactggtt ttatgagacg aggactattg
     3181 tcgcgagaat tttggagaaa gtatggcatt agaattgttg acgcctttta cgaaggtgga
     3241 attagaggaa gaaaaaaaag aaagcaaccg caaacagatt gggattttag gagggaattt
     3301 taatcctatt cacaatgcac accttgtggt tgcagatcaa gttcgccagc aattaggatt
     3361 agatcaggtg ctattgatgc ctgaatgtaa accaccgcat gtggatgcca aagagactat
     3421 tgatgagaag catcgtcttc gcatgttgga attggctatt gaagatgttg aagggctagc
     3481 tattgaaacc tgtgaacttg aacgtcaggg aatcagctat acctatgata cgatgcttta
     3541 tttgactgag cagcatcctg atgtcgatta ttattttatc atcggagctg atatggttga
     3601 ttatttgcca aagtggcacc gtattgatga attggtcaaa ctcgttcaat ttgttggggt
     3661 tcaacgtcca aaatataaag ctggaacctc ttacccagtg atttgggtag atcttccttt
     3721 gatggatatt tcttctagta tgattaggga ttttattaaa aaaggtcgtc agcccaatta
     3781 tctcttacct aagcgggttt tagactacat tactcaagaa ggtttgtatc aatgacatat
     3841 gaagattatc ttccttatag caggaccgaa ctgttagcaa aaattgcaga gcagatgagc
     3901 cctaaacggt ttaaacatgt acttggcgtg gaaaaggcag ccttgtcctt ggcagagtgt
     3961 tatggttgta acccagacaa agcaggatta gcagctcttt tacatgatta tgccaaggag
     4021 tgccctgacc aggtcttttt agacttaatt gacaagtacc aattatctcc agaattagct
     4081 aaatggaata ataatgtctg gcatggtatg gttggtattt ataagattca agaagactta
     4141 gggctaaaag acaaagatat cttgcgagct attgaaattc acaccgttgg tgctgctgag
     4201 atgaccttgc tggataaggt cttatacgtt gctgattata ttgaagaagg tcgcattttt
     4261 cctttggttg atgacgctag aaaaatcgcg aagctcgatt tgaatcaggc tgttgcctat
     4321 gaaacagtga acactgtcgc ttatttagcc tcaaaagcgc agcctatttt cccacaaact
     4381 ctggatacct ataatgcgtt ttgcagctat ttaaaggaat actaatggat aatgatatga
     4441 ggaataaaat gaaaaaagaa gaactcttaa aaattgttgt ggaagcaact gatgaaaagc
     4501 gtgctaaaga tattttagcc ttggatttag aggggttaac tagtttgact gattattttg
     4561 tgattgcgag tgcaacaaac agtcgtcaat tagaggctat tgcggataat attcgtgaaa
     4621 aggtaaaaga ggctggtgga gatgctagcc atgttgaagg taatagccaa gcaggctggg
     4681 tcttgcttga tttgaccgat gtggtggtac atcttttttt agaagacgag cgttaccact
     4741 ataatcttga aaaattgtgg catgaagcgc ctgctgtggc tctagatgcc tatttagctt
     4801 aatgttacaa agagaccttg tcaatacgac agggtttttg aggatgataa tggaacataa
     4861 ttatgaaaaa tttgcgtcag tttatgatgc tgtgatggat gattctctat atgacttgtg
     4921 gacagatttt tcacttcgac atttaccaaa gtctaagagt cgaaatcgtt tgttagagtt
     4981 ggcctgtgga accggtattc agtcagttcg ttttgctcaa gcaggttttg acgtgactgg
     5041 tttagactta agtcaagaca tgctggctat tgctaaaaag cgtgcgcaat ctgctaaaaa
     5101 gaaaattgat tttattcaag gtaacatgct ggatttgtca caggtggggc agtttgattt
     5161 tgtaacctgc tattcggatt ccatttgcta tatgcaagac gaagttgatg ttggcgatgt
     5221 ttttaaagaa gtttatgatg ttttagcaaa cgatggcatc tttatttttg atgtgcattc
     5281 aacctatcaa acggatgagt gtttccctgg ttattcctat catgagaatg cagatgattt
     5341 tgcaatggtt tgggatacct atgctgacga ggttcctcac tctgttgttc acgaactcac
     5401 tttctttatc caagaagacg atggacgttt ttctcgcttt gatgaagtgc atgaggaacg
     5461 aacgtatgag ttattgactt atgatatttt gttggaacaa gcagggttca agtcatttaa
     5521 ggtctatgcg gattttgagg ataaggagcc gactgagact agcaagcgtt ggttttttgt
     5581 ggcatataaa tagaagaaaa agtagccttt ttagctatag gatttaatga attatcttga
     5641 atgttactta tatgaaaaga gttcattagg cattaaatgc tattatcaaa gcttagaagt
     5701 attggagaca ttacgataaa gaaagcattt tctattatac gttgtgctta agcatttgat
     5761 ttaactctta ttagccctat aaaagattat ttagtacaat ttgaggagat tctatgactg
     5821 ttaccggaat tatagctgag tttaatccgt ttcacaatgg acacaaatat ttgctagaaa
     5881 cagcagaggg actaaaaatc attgctatgt caggaaattt tatgcaaaga ggagagcctg
     5941 ctcttattga taaatggatt cgatcagaga tggctcttaa aaatggtgca gatattgttg
     6001 tggaattgcc ctttttcgtt tcagttcaat cggcagacta ttttgctcaa ggagctattg
     6061 atattctttg tcaattagga attcagcaat tagcatttgg gacagaggat gtgattgatt
     6121 atcaaaaact gattaaggtc tatgagaaaa aatcagagca aatgactgcc tatttatcga
     6181 cgcttgaaga tacacttagc tatccacaaa aaacacaaaa aatgtgggaa atctttgctg
     6241 gagttaaatt ttcagggcaa acgccaaatc atattttggg gttaagttac gcaaaggcta
     6301 gcgctggaaa gcatatacaa ctatgtccta tcaagcgtca aggggctgcc taccattcga
     6361 aagacaaaaa tcatctgcta gcttctgcaa gtgccattag acagcacctg aatgattggg
     6421 atttcataag tcattcagta ccaaatgcag gtttacttat aaacaatccc catatgtcat
     6481 gggaccatta tttttcattc ttaaaatatc aaattttgaa ccattctgat ttaactagta
     6541 tttttcaggt caatgatgaa ttggcgagtc gtattaaaaa agcgataaaa gttagtcaaa
     6601 atattgacca cctagttgac actgttgcta ccaaacgcta tacaaaagct cgtgtaagac
     6661 gtattttaac ctacattttg gttaatgcta aggaacctac tttacctaag gggattcata
     6721 ttctagggtt tacctcaaaa ggacaagcac atttaaaaaa attaaaaaaa agcagacctc
     6781 tgattactag aattggagca gaaacttggg atgagatgac acaaaaagca gacagcatct
     6841 atcaacttgg gcatcaggat atcccagaac aaagttttgg aagaatacct attataataa
     6901 aaaatgag
//
LOCUS       DAUCLR010000092         6834 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.093, whole genome shotgun sequence.
ACCESSION   DAUCLR010000092 DAUCLR010000000
VERSION     DAUCLR010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6834
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.093"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            75..1127
                     /locus_tag="VGT87_001589"
     CDS             75..1127
                     /locus_tag="VGT87_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEQ3814424.1"
                     /translation="MNKKFIGLGLASVAVLSLAACGNRGASKGGAAGKTDLKVAMVTD
                     TGGVDDKSFNQSAWEGLQSWGKEMGLQKGTGFDYFQSTSESEYATNLDTAVSGGYQLI
                     YGIGFALKDAIAKAAGDNEGVKFVIIDDIIEGKDNVASVTFADHEAAYLAGIAAAKTT
                     KTKTVGFVGGMEGTVITRFEKGFEAGVKSVDDTIQVKVDYAGSFGDAAKGKTIAAAQY
                     AAGADVIYQAAGGTGAGVFNEAKAINEKRSEADKVWVIGVDRDQKDEGKYTSKDGKEA
                     NFVLASSIKEVGKAVQLINKQVADKKFPGGKTTVYGLKDGGVEIATTNVSKEAVKAIK
                     EAKAKIKSGDIKVPEK"
     gene            1266..2798
                     /locus_tag="VGT87_001590"
     CDS             1266..2798
                     /locus_tag="VGT87_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ3814425.1"
                     /translation="MTQHVIEMREITKKFGDFVANDHINLNVRKGEIHALLGENGAGK
                     STLMNMLAGLLEPTSGEIVINDKPVQIDSPSKSAKLGIGMVHQHFMLVEAFTVAENII
                     LGNEVVKNGCLDLNQASKDIKVLSEKYGLAINPSAKVSDISVGAQQRVEILKTLYRGA
                     DILIFDEPTAVLTPAEIKELMTIMKNLVKEGKSIILITHKLDEIRAVADRVTVIRRGK
                     SIETVDVAGATSQDLAEMMVGRSVSFTTSKKAAEPKDVVLSIKNLEVDENRGIPAVKG
                     LSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVKSGSIMIKNNEVTHLSSRKITELS
                     VGHVPEDRHRDGLILDLSLAENTALQTYYKQPLSQNGILNYTKINDYARQLMKEFDVR
                     GANELVPARGFSGGNQQKAIIAREVDRDPDLLIVSQPTRGLDVGAIEYIHKRLIKERD
                     KGKAVLVVSFELDEILNLSDRIAVIHDGKIQGIVLPENTNKQELGILMAGGSIYKEEG
                     HV"
     gene            2791..3855
                     /locus_tag="VGT87_001591"
     CDS             2791..3855
                     /locus_tag="VGT87_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ3814426.1"
                     /translation="MSKNAQKIAVPLISVLLGFLLGAIIMVIFGYDPIWGYEGLFQIA
                     FGSVKNIGEIFRSMGPLILIALGFTVASRAGFFNVGLSGQALAGWISAGWFALLNPDM
                     PRPLLILMTALIGMVAGGIAGAIPGILRAYLGTSEVIVTIMMNYIILYVGNAIVQRGY
                     PESVKQSIDSTIQVSDNASYQTHWLSALTNNSRINIGIFFAIIAIALIWFLLNKTTLG
                     FEIRSVGLNPHASEYAGMSSKRTIILSMIISGALAGLGGVVEGLGTFENVFVQGSSLA
                     VGFDGMAVSLLAANSPLGIFFSSFLFGVLNIGAPGMNIAGIPPELVKVVTASIIFFVG
                     SHYLIERYIIRPKKLVKGGK"
     gene            3857..4813
                     /locus_tag="VGT87_001592"
     CDS             3857..4813
                     /locus_tag="VGT87_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ3814427.1"
                     /translation="MSLVTIFALLVSSMLIYATPLIFTSIGGTFSERSGVVNVGLEGI
                     MVMGAFSGIVFNLEFAETFGKATPWIAVLVGGIVGLIFSLIHAVATINFRADHIVSGT
                     VLNLLAPSFAVFLVKAMYGKGQTDNIQQSFGKFDFPGLSQIPVIGDIFFKNTSLIGYF
                     AIAFSFFAWFLLYKTRFGLRLRSVGEHPQAADTLGINVYLMKYYGVMISGFLGGIGGA
                     VYAQSISVNFAVTTILGPGFIALAAMIFGKWNPVGAMLSSLFFGLSQSLAVIGAQLPL
                     LEKIPTVYLQIAPYMVTIIILAAFFGQAVAPKADGINYIKSK"
     gene            complement(5026..6744)
                     /locus_tag="VGT87_001593"
     CDS             complement(5026..6744)
                     /locus_tag="VGT87_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEQ3814428.1"
                     /translation="MTYTENFQKWLDFEQLPDYLRQELLSMDEKTKEDAFYTNLEFGT
                     AGMRGYIGAGTNRINIYVVRQATEGLAKLIETKGEEAKKRGVAIAYDSRHFSPEFAFE
                     SAQVLAQHGIKSYVFEALRPTPELSFAVRHLNAYAGIMVTASHNPAPFNGYKVYGQDG
                     GQLPPADADALTDFIRAIENPFAVELADLDESKSAGLIQVIGEDVDMEYLREVKDVNI
                     NQDLINNFGKDMKIVYTPLHGTGEMLTRRALAQAGFESVVVVESQAKADPDFSTVKSP
                     NPESQAAFALAEELGREVDADVLVATDPDADRLGVEIRQPDGSYKNLSGNQIGAIIAK
                     YILEAHKTAGTLPENAALAKSIVSTELVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMFGFEESFGYLIKPFVRDKDAIQAVLLVAEIAAYYRSRGLTLADGIDEIYKE
                     YGYFAEKTISVTLSGVDGAAEIKKIMNKFRENGPKQFNNTDIVLLEDFQKQTATKNDG
                     TISNLTTPPSNVLKYTLADDSWIAVRPSGTEPKIKFYIATIGDTLDIAQEKIANIETE
                     INTFVG"
BASE COUNT         2033 a         1160 c         1419 g         2222 t
ORIGIN      
        1 tctagccgtc ttggctttat atttactttc agcaacttat ttgctagaaa tcttttttag
       61 gagggttcat attcatgaac aagaaattta ttggtcttgg tttagcgtca gtggctgtgc
      121 tgagtttagc tgcttgtggt aatcgtggtg cttctaaagg tggggcagca ggaaaaactg
      181 atttaaaagt cgcaatggtt accgatactg gtggtgtaga tgacaaatca ttcaaccaat
      241 cagcatggga aggcctgcaa tcttggggta aagaaatggg ccttcaaaaa ggaacaggtt
      301 tcgattattt tcaatctaca agtgaatctg agtatgcaac taatcttgat acagcagttt
      361 caggagggta tcaactgatt tatggtatcg gctttgcatt gaaagatgct attgctaaag
      421 cagctggaga taatgaagga gttaagtttg ttattatcga tgatattatc gaaggaaaag
      481 ataatgtagc cagtgttacc tttgctgacc atgaagctgc ttatcttgca ggaattgcag
      541 ctgcaaaaac aacaaaaaca aaaacagttg gtttcgtggg cggtatggaa ggaactgtca
      601 taactcgatt tgaaaaaggt tttgaagcag gagttaagtc tgttgacgat acaatccaag
      661 ttaaagttga ttatgctgga tcatttggtg acgctgcaaa aggaaaaaca atcgcagcag
      721 ctcagtatgc agcaggtgct gatgttattt accaggcagc aggaggcact ggagcaggtg
      781 tatttaatga agcaaaagct attaatgaaa aacgtagtga agctgataaa gtttgggtta
      841 ttggtgttga ccgtgatcaa aaagacgaag gaaaatacac ttctaaagat ggcaaagaag
      901 caaactttgt acttgcatca tcaatcaaag aagttggtaa agctgttcag ttaatcaaca
      961 agcaagtagc agataaaaaa ttccctggag gaaaaacaac tgtctatggt ctaaaagatg
     1021 gcggtgttga aatcgcaact acaaatgttt caaaagaagc tgttaaagct attaaagaag
     1081 cgaaagcaaa aattaaatct ggtgacatta aagttcctga aaaatagtgt ttaagaacaa
     1141 gaagcgcgac ctttgtcggt cgctcttttt aaactgagat aatttttatc tcagtaaagt
     1201 ttgtgatagg tgatagatca taggctttac tgaaataaaa gattgcattg aaaggaaatg
     1261 atcctatgac acagcatgtc attgaaatga gagaaatcac gaaaaaattt ggtgattttg
     1321 ttgcaaatga ccacattaac ttaaatgtca gaaaaggtga gattcatgcc ttattgggtg
     1381 aaaatggggc aggtaaatca actttgatga atatgctagc tggtctctta gagcctacta
     1441 gtggagaaat tgtcattaat gacaagccag ttcaaattga ttcgccatct aaatcagcta
     1501 agcttggtat cggaatggta catcaacatt tcatgttagt agaagccttc acggttgctg
     1561 agaatattat tttaggaaat gaagtagtaa aaaatgggtg cttagatctt aaccaagcat
     1621 cgaaagatat taaggtacta tccgaaaaat atggccttgc aatcaatcca tcagctaaag
     1681 tttctgatat ttcagtagga gcacaacaac gtgttgagat tttgaaaacc ttgtatcgtg
     1741 gtgcagatat tcttatcttt gatgagccaa ctgccgtttt aacacctgca gaaattaaag
     1801 aattgatgac tatcatgaag aatctcgtga aagaaggaaa atcaattatt ttaattaccc
     1861 ataaactgga cgagattcgt gctgtcgccg accgtgtcac agttattcgt cgtggtaaga
     1921 gtatcgaaac agttgatgtg gcaggagcaa cttcacaaga tttagctgag atgatggtgg
     1981 gacgctcggt ttcctttaca actagcaaaa aagctgctga accaaaagat gtggtcttat
     2041 ccatcaaaaa tcttgaagta gatgaaaata gagggattcc agcagttaag gggctatctc
     2101 tagatgtccg agctggtgaa attgtaggga ttgctggaat tgacggaaat ggtcaatctg
     2161 agttaattca agctattaca ggtttacgta aagtcaaatc tggaagtatc atgattaaaa
     2221 acaatgaagt cactcatttg tcttcacgca aaattactga gttatcagtg ggtcacgtgc
     2281 cagaagatcg tcatcgagat ggtttgatac tagatctttc tttagctgaa aatacagcct
     2341 tacaaaccta ttacaaacaa cctctaagtc aaaatggaat cttaaattat actaaaatta
     2401 atgactatgc tcgtcaatta atgaaagaat ttgatgttcg cggagccaat gagctagtac
     2461 cagctagagg attttctggt ggtaaccaac aaaaggccat tattgctcgt gaagtggacc
     2521 gtgatccaga tttacttatt gttagccaac caacacgtgg tcttgacgta ggagctattg
     2581 aatatatcca taagcgtttg attaaagaac gtgataaagg taaagctgtc ctcgttgtta
     2641 gttttgaact tgatgaaata ttgaatttat ctgatcgaat tgctgttatt catgatggta
     2701 aaattcaagg tatcgtattg ccagaaaata ccaataaaca agaattgggt attttaatgg
     2761 caggtggttc aatttataag gaggaaggtc atgtctaaaa atgcgcaaaa aattgctgta
     2821 ccattgattt ctgtcttgct aggattttta cttggcgcta ttattatggt tatctttggt
     2881 tatgatccta tctgggggta tgaaggttta ttccaaattg cctttggtag tgttaaaaat
     2941 attggagaaa tattccgttc aatggggcca ttgattttaa tagcgcttgg ctttactgtt
     3001 gctagccgtg caggcttctt taacgttggt ttgtctggac aggctttagc tggatggatt
     3061 tcagcaggtt ggtttgcact cttaaatcca gatatgcctc gtccattatt gattcttatg
     3121 acagctctta tcggaatggt tgctggtggt attgctggag ctattccagg gatacttcgt
     3181 gcttatcttg gtacaagtga agttatcgtt acgattatga tgaactacat cattttatat
     3241 gtcggaaatg ctattgttca acgtggttat cctgagagtg tcaaacaaag tattgattca
     3301 actattcaag ttagtgataa tgctagttat caaacgcatt ggttatcagc cctaactaat
     3361 aactctcgta ttaatattgg tattttcttt gccattattg ccattgccct aatctggttc
     3421 ttgcttaaca aaactacttt aggttttgag attcgttcag ttggtctcaa cccacatgct
     3481 tcagaatacg cagggatgtc atcaaaacgt actatcattt tatcaatgat tatctctgga
     3541 gctcttgcag gtcttggagg agtagttgaa ggtctaggaa cctttgaaaa tgtttttgta
     3601 caaggatctt ctttagcagt tggttttgac ggtatggcag ttagcttatt agcagcaaat
     3661 tcaccgcttg gaatattttt ctcatcattc ctatttggtg tcttaaatat tggtgctcca
     3721 ggaatgaata ttgcaggcat tccaccagaa cttgttaaag tagtaactgc atcaatcatt
     3781 ttctttgttg gttcacatta cctcattgaa cgttacatta ttcgccctaa aaaattagtg
     3841 aaaggtggta aataagatga gtttagtaac aatttttgcc ttattggtgt catctatgct
     3901 tatttatgca acaccactca ttttcacaag tattggagga actttctcag agcgctcagg
     3961 cgttgttaat gttggtttag aaggtatcat ggtcatggga gcgttctctg ggattgtttt
     4021 caacttagag tttgctgaaa catttggaaa agcaacgcct tggatagccg ttttagtcgg
     4081 tggtattgtt ggtttgattt tttctttgat tcacgctgta gcaaccatca actttcgagc
     4141 tgaccacatt gtcagtggta cagtgttgaa cttgttagca ccttcttttg ctgtcttctt
     4201 ggttaaagct atgtacggta agggacaaac agacaacatt caacaatctt ttgggaagtt
     4261 tgatttccca ggtttatcac aaattcctgt gattggtgat attttcttta aaaatactag
     4321 ccttattggt tacttcgcca ttgctttttc gttctttgct tggtttttgc tttataaaac
     4381 caggtttggt ctgcgtttac gatcagttgg ggaacaccct caggcagctg acacacttgg
     4441 tattaacgtc tatttgatga aatactatgg tgtgatgatc tcaggttttc ttggtggaat
     4501 aggtggagct gtttatgctc agtcaatctc cgttaacttt gctgtaacaa ctatcttagg
     4561 tcctggattt attgctttgg cagctatgat ctttggtaaa tggaatccag ttggcgccat
     4621 gctatctagt cttttctttg gcttatcgca aagtttagcc gttattggtg ctcaattacc
     4681 attactagaa aagattccaa cggtttactt acagattgca ccttatatgg taacgattat
     4741 tattttagct gctttctttg gtcaagcagt tgcaccaaaa gctgatggaa tcaactatat
     4801 taaatctaaa taacgttata ttagtaagcc tcttagtgag gtttaacagc actcctaaag
     4861 caagatagtt aggtctagct ttaagggtgc ttttttggga cttaccgcaa gtttggattg
     4921 ccagtcacca tcacaataga agcaagaata gactaaaaag aactaagtca gcggcttagt
     4981 tctttttgct tgtctttcgt ttttactgat agctcttaag ttgttttagc caacaaaggt
     5041 attgatttct gtttcaatgt tagcaatttt ttcttgagcg atgtcaagag tatcacctat
     5101 tgtggcaata tagaacttga tttttggttc agttcctgaa gggcgaactg caatccatga
     5161 atcatcagct aacgtgtact tcaaaacatt acttggagga gttgttaagt tagatattgt
     5221 tccatcattt ttagtagcgg tttgtttttg gaaatcttct aataatacaa tatcagtatt
     5281 gttaaattgt tttggaccat tttcacggaa tttgttcata atcttcttaa tttcagcagc
     5341 tccatccaca cctgaaagtg ttactgaaat cgttttttca gcaaaatagc catattcttt
     5401 gtaaatttca tcaataccat ctgcaagtgt taaaccacgc gaacggtaat aagctgcgat
     5461 ctcagcaacc aaaagaactg cttgaatagc gtctttatca cggacaaatg gtttaataag
     5521 atatccgaag ctttcttcaa aaccaaacat ataggtgtgg ttatgttttt cttcaaactc
     5581 ttgaattttc tcagcgatga atttgaatcc agttaaaacg ttaaacattg tagcgccata
     5641 actttcggca atttttgtta ctaattctgt tgatacgatt gatttagcga gagcagcatt
     5701 ttctggaagt gttcctgctg ttttatgagc ttccaggatg tatttcgcaa taatcgctcc
     5761 aatttgattt ccagaaagat ttttataaga accatctggt tggcgaattt caacgcctag
     5821 gcggtctgca tcaggatcgg tagctactaa gacatctgca tcaacttctc gtccgagttc
     5881 ttcagcaaga gcaaaggcag cttggctttc agggttaggc gatttaacag tagaaaagtc
     5941 tggatctgcc ttagcttgtg attctactac aacaacagat tcaaaaccgg cttgagcaag
     6001 ggcacggcgt gtcaacatct caccagttcc gtggagagga gtatagacaa ttttcatgtc
     6061 ttttccaaag ttattgatta aatcttgatt gatattaaca tctttaacct ctctaagata
     6121 ttccatatca acatcttcac cgataacttg aataagacct gctgatttgc tttcatctaa
     6181 atcagcaagt tcaactgcaa atggattttc aatagcacgg ataaagtctg ttagtgcatc
     6241 agcatcagct ggtggtagtt gacctccgtc ttgtccatag actttataac cattgaaagg
     6301 tgctggattg tggcttgcag taaccataat accagcatag gcatttaaat ggcgaacagc
     6361 aaaggagagt tctggtgttg gacgaagagc ttcaaatacg taagatttaa ttccatgctg
     6421 tgccaagacc tgtgcagact caaaagcaaa ttctggagag aagtggcgcg aatcataagc
     6481 gatggctaca ccacgttttt tagcttcctc tcccttagtt tctattaatt ttgctaatcc
     6541 ttcggttgct tgacggacaa cataaatatt aatacgattt gtaccagcac caatataacc
     6601 acgcatacca gcagtaccaa attctaagtt tgtgtaaaaa gcatcttctt ttgttttttc
     6661 atccattgat agtaattctt gacgaagata gtcaggaagc tgctcaaaat ctaaccattt
     6721 ttgaaaattt tctgtgtaag tcattgaaat actccttcaa tatttataaa cgctttcatt
     6781 atatcatttt gaagggaaaa aaacactaat tatttgcagc tgtttacaaa actt
//
LOCUS       DAUCLR010000093         6688 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.094, whole genome shotgun sequence.
ACCESSION   DAUCLR010000093 DAUCLR010000000
VERSION     DAUCLR010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6688
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.094"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            143..3253
                     /locus_tag="VGT87_001594"
     CDS             143..3253
                     /locus_tag="VGT87_001594"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEQ3814429.1"
                     /translation="MFAQLDTKTVYSFMDSLIDLNHYFERAKQFGYHTIGIMDKDNLY
                     GAYHFIKGCQKNGLQPVLGLEVEILYQERQVLLNLIAQNTQGYHQLLKISTAKMSGKL
                     HMDYLCQHLEGIAVIIPSKGWSDTLVVPFDYYIGVDQYTDLSHMDSKRQLIPLRTVRY
                     FAQDDMETLHMLHAIRDNLSLAETPVVESDQELTDCQQLTTFYQTHCPQALQNLEDLV
                     SGIYYDFDTNLKLPHFNRDKSAKQELQELTEAGLKEKGLWKEPYQSRLLHELVIISDM
                     GFDDYFLIVWDLLRFGRSKGYYMGMGRGSAAGSLVAYALNITGIDPVQHDLLFERFLN
                     KERYSMPDIDIDLPDIYRSEFLRYVRNRYGSDHSAQIVTFSTFGPKQAIRDVFKRFGV
                     PEYELTNLTKKIGFKDSLATVYEKSISFRQVINSRTEFQKAFAIAKRIEGNPRQTSIH
                     AAGIVMSDDTLTNHLPLKSGDDMMITQYDAHAVEANGLLKMDFLGLRNLTFVQKMQEK
                     VAKDYGCQIDIAAIDLEDPQTLALFAKGDTKGIFQFEQNGAINLLKRIKPQRFEEIVA
                     TTSLNRPGASDYTTNFIKRREGQEKIDLIDPVIAPILEPTYGIMLYQEQVMQIAQIYA
                     GFTLGKADLLRRAMSKKNLQEMQKMEEDFIASAKHLGRAEETARGLFKRMEKFAGYGF
                     NRSHAFAYSALAFQLAYFKAHYPAVFYDIMMNYSSSDYITDALESDFQVAQVTINSIP
                     YTDKIEASKIYMGLKNIKGLPRDFAYWIIEQRPFNSVEDFLTRTPEKYQKKVFLEPLI
                     KIGLFDCFEPNRKKILDNLDGLLVFVNELGSLFSDSSFSWVDAKDYSATEKYSLEQEI
                     VGVGMSKHPLIDIAEKSTQTFTPISQLVKESEAVVLIQIDSIRIIRTKTSGQQMAFLS
                     VNDTKKKLDVTLFPQEYAIYKDQLKEGEFYYLKGRIKERDHRLQMVCQQVQMAISQKY
                     WLLVENHQFDSQISEILGAFPGTTPVVIHYQKNKETIALTKIQVHVTENLKEKLRPFV
                     LKTVFR"
     gene            3333..4346
                     /gene="pfkA"
                     /locus_tag="VGT87_001595"
     CDS             3333..4346
                     /gene="pfkA"
                     /locus_tag="VGT87_001595"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEQ3814430.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINRGYAGM
                     VDGDIFPLGSKEVGDKISRGGTFLYSARYPEFAQLEGQLAGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAVGIPGTIDNDIAGTDYTIGFDTAVNTAVEAIDKLRDTSSSH
                     GRTFVVEVMGRNAGDIALWAGIASGADQIIVPEEEFDIEKVASTIQYDFEHKGKNHHI
                     IVLAEGVMSGEAFAQKLKEAGDKSDLRVTNLGHILRGGSPTARDRVIASWMGSHAVEL
                     LKEGKGGLAVGIHNEELVESPILGTAEEGALFSLTEEGKIIVNNPHKARLDFAALNRS
                     LSQ"
     gene            4409..5911
                     /gene="pyk"
                     /locus_tag="VGT87_001596"
     CDS             4409..5911
                     /gene="pyk"
                     /locus_tag="VGT87_001596"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEQ3814431.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKYGEDGYWAGQLDVEESAKKIAE
                     LIEAGANVFRFNFSHGDHKEQGDRMATVRLAEEIARQKVGFLLDTKGPEMRTELFADD
                     AKEFSYVTGEKIRVATTQGIQSTRDVIALNVAGSLDIYDEVEVGHTILIDDGKLGLKV
                     IDKDIATRQFIVEVENDGIIAKQKGVNIPNTKIPFPALAERDNADIRFGLEQGLNFIA
                     ISFVRTAKDVEEVREICRETGNDHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVFQKMIITKVNAAGKAVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPVESVRTMATIDRNAQTLLNEYGRLDSSAFPRTNKTDVIASAVK
                     DATHSMDIKLVVTITETGNTARAISKFRPDADILAVTFDEKVQRALMINWGVIPVLAE
                     KPASTDDMFEVAERVAVEAGLVQSGDNIVIVAGVPVGTGGTNTMRVRTVK"
     gene            6129..6686
                     /gene="lepB"
                     /locus_tag="VGT87_001597"
     CDS             6129..6686
                     /gene="lepB"
                     /locus_tag="VGT87_001597"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEQ3814432.1"
                     /translation="MVKRDFIRNILLLLIVIIGAILLRIFVFSTFKVSPETANTYLKS
                     GDLVTIKKNIQPKYKDFVVYRVGKKDYVSRVIAVEGDSVTYMDDIFYLNNMVESQAYL
                     EKMKAHYLNHAPFGTLYTDDFTVATITADKYQKVPKGKYLLLNDNRKNTNDSRRFGLI
                     NASQIKGLVTFRVLPLSDFGFVEVE"
BASE COUNT         2101 a         1107 c         1431 g         2049 t
ORIGIN      
        1 atggtacaga aaaaaaaagg gaatgtcaag ttttatgcca tttttcactc tttgagaagt
       61 agacgtagat tagagagaaa attgaagcaa gaaaaccttt ttcatagtat aatagggaac
      121 aagataacca aaggaggaac ttatgtttgc tcaacttgat actaaaactg tatactcatt
      181 tatggatagt ttaattgact taaatcatta ttttgaacga gcaaagcaat ttggttacca
      241 caccatagga atcatggata aggataatct ttatggtgct taccatttta ttaaaggttg
      301 tcaaaaaaat ggactgcagc cagttttagg tttggaagta gagattctct atcaagagcg
      361 gcaggtgctc cttaacttaa tcgcccagaa tacacaaggc tatcatcagc ttttaaaaat
      421 ttccacggca aaaatgtctg gcaagcttca tatggattac ctctgccaac atttggaagg
      481 gatagcggtt attattccta gtaagggttg gagcgataca ttagtggtcc cttttgacta
      541 ctatatcggt gttgatcagt atactgattt atctcatatg gattctaaga ggcagcttat
      601 acccctaagg acagttcgtt attttgcgca agatgatatg gaaaccctgc acatgttgca
      661 tgccattcga gataacctca gtctggcaga gacccctgtg gtagaaagtg atcaagagtt
      721 aacagattgt caacaactaa ccaccttcta tcaaacacac tgccctcaag ctctacagaa
      781 tttagaagac ttagtgtcag gaatctatta tgatttcgat acaaatttaa aattgcctca
      841 ttttaataga gataagtctg ccaagcaaga attgcaagag ttgactgagg ctggtttgaa
      901 ggaaaaagga ttgtggaaag agccttatca atcgcgctta ctgcatgaat tggtcattat
      961 ttctgacatg ggctttgatg attatttttt gattgtgtgg gatttacttc gctttggacg
     1021 cagtaaaggc tattatatgg gtatgggacg tggctcggcg gcaggtagtc tagtggctta
     1081 tgctctgaac attacaggga ttgatccagt tcaacatgat ttgctatttg agcgcttttt
     1141 aaacaaagaa cgttatagca tgcctgatat tgatatcgat cttccagata tttaccgttc
     1201 agaatttcta cggtatgtcc gaaatcgtta tggtagcgac cattcggcgc aaattgtgac
     1261 cttttcaacc tttggaccta aacaggctat tcgtgatgtt ttcaaacgat tcggggttcc
     1321 agaatacgaa ctgaccaatc tcactaaaaa aattggtttt aaagatagct tggctactgt
     1381 ctatgaaaag tcaatctctt ttaggcaggt tattaatagt agaactgaat ttcaaaaggc
     1441 ttttgccatt gccaagcgta tcgaaggaaa tccaagacaa acgtccattc atgcagctgg
     1501 tattgtgatg agtgatgata ccttgaccaa tcatcttcct ctaaaatcgg gcgatgacat
     1561 gatgatcacc cagtatgatg ctcatgcggt cgaagctaat ggcctgttaa aaatggattt
     1621 tttggggtta agaaatttga cctttgttca aaaaatgcaa gagaaggttg ctaaagacta
     1681 cgggtgtcag attgatattg cagccattga tttagaagac ccgcaaacgt tggcactttt
     1741 tgctaaaggg gataccaagg gaattttcca atttgaacaa aatggtgcta ttaatctttt
     1801 aaaacggatt aagccacaac gttttgaaga aattgttgcc actaccagtc taaatagacc
     1861 aggggcaagt gactatacca ctaatttcat taaacgaaga gaaggacaag aaaaaattga
     1921 tttgattgat cctgtgattg ctcccatttt agagccaact tacggtatta tgctttatca
     1981 agaacaagtt atgcagattg cacagattta tgctggtttt acgttaggca aggccgactt
     2041 gttaaggcgt gccatgtcta aaaaaaatct acaagaaatg caaaaaatgg aagaagactt
     2101 tattgcttct gctaagcacc tagggagagc tgaagaaaca gctagaggac tttttaaacg
     2161 gatggaaaaa tttgcaggtt atggttttaa ccgcagccat gcctttgcct attcagcttt
     2221 agcttttcaa ttggcttatt tcaaagccca ttatccggct gttttttacg atatcatgat
     2281 gaattattct agcagtgact atatcacaga tgctctagaa tcagattttc aagtagcgca
     2341 agttaccatt aatagtattc cttacactga taaaattgaa gctagcaaga tttacatggg
     2401 gctgaaaaat attaaggggt tgccaaggga ttttgcttat tggattatcg agcaaagacc
     2461 atttaatagc gtagaggatt ttctcactag aactccagaa aaatatcaaa aaaaggtttt
     2521 ccttgagcct ctgataaaaa taggtctgtt tgattgcttt gagcctaacc gtaaaaaaat
     2581 tctggacaat ttggatggtt tactggtatt tgttaatgag cttggttctc ttttttcaga
     2641 ttcttccttt agttgggtag atgcgaaaga ttactcagca actgaaaaat attctttgga
     2701 acaggagatc gttggggttg gcatgagcaa gcatccttta attgatattg ctgagaaaag
     2761 tacccaaact tttactccca tttcacagtt agtcaaagaa agcgaagcag tcgtactgat
     2821 tcaaatagat agcattagga tcattagaac caaaacaagt gggcagcaaa tggctttttt
     2881 aagtgtgaat gacactaaga aaaagctcga tgtcacactt tttccacaag agtatgctat
     2941 ttataaagac caattaaaag aaggagaatt ctattactta aaaggtagaa taaaagaaag
     3001 agaccatcga ctgcagatgg tgtgtcagca agtgcaaatg gctattagtc aaaaatattg
     3061 gttattagtt gaaaaccatc agtttgattc ccaaatttct gagattttag gtgcctttcc
     3121 aggaacgaca ccagttgtta ttcactatca aaaaaataag gaaacaattg cattaactaa
     3181 gattcaggtt catgtaacag agaatttaaa ggaaaaactt cgtccttttg ttctgaaaac
     3241 ggtttttcga taaaaatgga caattagtga agtattttgc aaatgaatgt gctataataa
     3301 gaccgttaaa ttaattaaag gagtaccaga aaatgaaacg tattgctgtt ttaaccagtg
     3361 gcggcgacgc tcctggtatg aatgctgcta ttcgtgcagt cgttcgtaaa gcaatttcag
     3421 aaggaatgga agtttatggt atcaaccgtg gctatgcagg tatggttgat ggcgacattt
     3481 tcccactggg ttcaaaagaa gttggtgata aaatctcacg tggtggaact ttcttatact
     3541 cagctcgtta tccagaattt gctcaacttg aaggtcaatt ggctggtatt gagcaactta
     3601 aaaaacatgg tattgagggt gttgttgtta ttggtgggga tggttcttat catggcgcta
     3661 tgcgcttgac agagcacggc ttcccagcgg taggtatccc aggaacaatc gataacgata
     3721 ttgcgggaac tgactacact atcggatttg atacagcagt taacacagca gttgaagcta
     3781 ttgataaact tcgtgacacc tcatcaagtc acggacgtac ttttgtggtt gaagtgatgg
     3841 gtcgcaatgc gggtgatatt gctctttggg caggtattgc tagtggtgct gatcaaatca
     3901 ttgttccaga agaagagttt gatattgaaa aagtggcatc aacaatccaa tatgattttg
     3961 aacacaaagg taaaaatcac cacattatcg tcttagctga aggtgttatg agcggtgaag
     4021 cttttgctca aaaactgaaa gaagctggcg ataagagtga ccttcgtgtg acaaatcttg
     4081 gtcacattct tcgtgggggt tcaccaactg ctcgtgaccg tgtcattgct tcttggatgg
     4141 gttcacatgc tgttgagcta cttaaagaag gcaaaggtgg tttggctgtt ggtattcaca
     4201 atgaagaact tgttgaaagc ccaatccttg gtactgcaga agaaggtgct ttgttcagct
     4261 tgactgaaga aggcaagatt attgttaata atccacacaa agctcgttta gactttgcag
     4321 ctttgaaccg ttcattgtca caataagttt agttaaaatt gttaaatcgt caattattga
     4381 caaaaaaata agggagtttc agagtaaaat gaataaacgc gtaaaaatcg ttgcaacact
     4441 tggtcctgcg gttgaaatcc gtggtggtaa aaaatacgga gaagatggct actgggctgg
     4501 tcaacttgat gttgaagaat cagcaaaaaa aatcgctgaa ttaattgaag ctggtgcgaa
     4561 tgttttccgt ttcaatttct cacatggtga ccacaaagaa caaggtgacc gtatggcaac
     4621 tgttcgcctt gcagaagaaa ttgctcgtca aaaagttggt ttccttttgg atactaaagg
     4681 tcctgaaatg cgtacggaat tgtttgcaga tgatgctaaa gaattcagct atgtcactgg
     4741 agaaaaaatc cgtgtagcga ctacacaagg tattcaatca actcgcgatg ttattgcttt
     4801 gaacgttgct ggtagccttg acatctacga tgaagtagaa gtaggtcata ctattcttat
     4861 cgatgatggt aaactcggtt tgaaagtgat cgacaaagat atcgcaacac gtcaatttat
     4921 cgttgaagta gaaaacgatg gtattattgc taaacaaaaa ggtgttaata tccctaacac
     4981 taaaattcct ttcccagcac ttgcggaacg cgacaatgct gatatccgtt ttggtcttga
     5041 acaaggactt aactttattg caatctcatt tgttcgtaca gccaaagacg ttgaggaagt
     5101 tcgtgaaatc tgtcgcgaaa ctggtaacga tcatgtgcaa ttatttgcta agattgaaaa
     5161 ccaacaaggt atcgataatc ttgatgaaat catcgaagct gctgatggta tcatgattgc
     5221 acgtggcgac atgggtatcg aagtaccatt tgaaatggtt ccagttttcc aaaaaatgat
     5281 tatcactaaa gtgaatgcag ctggtaaagc agttatcaca gcaacaaata tgcttgaaac
     5341 aatgactgaa aaaccacgtg cgacacgttc tgaagtatct gacgtcttca atgctgttat
     5401 cgatggtact gatgcaacaa tgctttcagg agagtcagct aacggtaaat acccggttga
     5461 atctgttcgt acaatggcga ctattgatcg taatgcacaa actcttctta acgaatatgg
     5521 tcgtttagat tcatctgcct tcccacgtac caataaaaca gatgttattg cttcagctgt
     5581 taaagatgcc actcattcaa tggacatcaa acttgttgtg actattactg agacaggtaa
     5641 tacagctcgt gcgatttcta aattccgtcc agatgcggat atcttagctg taacatttga
     5701 tgaaaaagta caacgtgctt tgatgattaa ctggggtgtt attcctgttc ttgctgaaaa
     5761 acctgcatct actgatgaca tgtttgaagt agcagagcgt gttgctgtgg aggcaggctt
     5821 agttcaatct ggggataata ttgttattgt tgctggtgtt ccagttggta ctggtggaac
     5881 gaatactatg cgtgttcgta cagtaaaata aatgtgttat aactatttac ctaaaggtta
     5941 gaaggcaagc ttctaacctt ttttgatatc aaaaatatca tgttaattca ctagtgttaa
     6001 tttactttaa tgagtctaag tttgtatttt atacagaatg agtaacgttt attggtattg
     6061 atcaaaaatt gtttactttt tttctagctt agaacatggt ataataagac aatagactag
     6121 gagttaatat ggtaaagcga gattttataa gaaatatatt attgttatta attgtgatta
     6181 ttggggctat tttgttgaga atctttgttt tttcaacttt taaagtttct ccagaaacag
     6241 ctaatactta tttaaagagt ggtgatttag tcacaatcaa aaaaaatatt cagcccaaat
     6301 ataaagattt tgtggtttat agagttggaa aaaaagatta tgtcagtcga gtcattgctg
     6361 ttgaaggcga tagcgtgact tatatggatg atatttttta cctcaataat atggtagagt
     6421 cacaggctta ccttgaaaag atgaaagcac attacttgaa tcatgcaccg tttggcacat
     6481 tgtatacaga tgattttaca gttgctacca tcacagctga taagtatcaa aaagttccta
     6541 aggggaagta tcttcttttg aatgataacc ggaaaaatac gaatgacagt cgtcgatttg
     6601 ggttaataaa tgcctcgcag attaaaggtt tagtgacctt tagagttttg cctctcagcg
     6661 attttggatt tgtagaagta gagtagtt
//
LOCUS       DAUCLR010000094         6502 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.095, whole genome shotgun sequence.
ACCESSION   DAUCLR010000094 DAUCLR010000000
VERSION     DAUCLR010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6502
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.095"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..887)
                     /gene="hasC"
                     /locus_tag="VGT87_001598"
     CDS             complement(<1..887)
                     /gene="hasC"
                     /locus_tag="VGT87_001598"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     HasC"
                     /protein_id="HEQ3814433.1"
                     /translation="MTKVRKAIIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEE
                     ALKSGIEEILVVTGKAKRSIEDHFDSNFELEYNLQAKGKNELLKLVDETTAINLHFIR
                     QSHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITNASAKPLTKQLMEDYDKTHASTI
                     AVMKVPHEDVSSYGVIAPQGKAVKGLYSVDTFVEKPQPEDAPSDLAIIGRYLLTPEIF
                     DILERQTPGAGNEVQLTDAIDTLNKTQRVFAREFKGNRYDVGDKFGFMKTSIDYALEH
                     PQVKEDLKNYIIKLGKALE"
     gene            complement(1069..2277)
                     /gene="hasB"
                     /locus_tag="VGT87_001599"
     CDS             complement(1069..2277)
                     /gene="hasB"
                     /locus_tag="VGT87_001599"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase HasB"
                     /protein_id="HEQ3814434.1"
                     /translation="MKIAVAGSGYVGLSLGVLLSLQNEVTIVDILPSKVDKINNGLSP
                     IQDEYIEYYLKSKQLSIKATLDSKAAYKEAELVIIATPTNYNSRINYFDTQHVETVIK
                     EVLSVNSHATLIIKSTIPIGFITEMRQKFQTDRIIFSPEFLRESKALYDNLYPSRIIV
                     SCEENDSPKVKADAEKFALLLKSAAKKNNVPVLIMGASEAEAVKLFANTYLALRVAYF
                     NELDTYAESRKLNSHMIIQGISYDDRIGMHYNNPSFGYGGYCLPKDTKQLLANYNNIP
                     QTLIEAIVSSNNVRKSYIAKQIINVLEERESPVKVVGVYRLIMKSNSDNFRESAIKDV
                     IDILKSKDIKIIIYEPMLNKLESEDQSVLVNDLENFKKQANIIVTNRYDNELQDVKNK
                     VYSRDIFNRD"
     gene            complement(2313..3572)
                     /gene="hasA"
                     /locus_tag="VGT87_001600"
     CDS             complement(2313..3572)
                     /gene="hasA"
                     /locus_tag="VGT87_001600"
                     /EC_number="2.4.1.212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronan synthase HasA"
                     /protein_id="HEQ3814435.1"
                     /translation="MPIFKKTLIVLSFIFLISILIYLNMYLFGASTVGIYGVILITYL
                     VIKLGLSFLYEPFKGKPHDYKVAAVIPSYNEDAESLLETLKSVLAQTYPLSEIYIVDD
                     GSPNTDAIQLIEEYVNREVDICRNVIVHRSLVNKGKRHAQAWAFERSDADVFLTVDSD
                     TYIYPNALEELLKSFNDKTVYAATGHLNARNRQTNLLTRLTDIRYDNAFGVERAAQSL
                     TGNILVCSGPLSIYRREVIIPNLERYKNQTFLGLPVSIGDDRCLTNYAIDLGRTVYQS
                     TARCDTDVPFQLKSYFKQQNRWNKSFFRESIISVKKILSNPIVALWTIFEVVMFMMLI
                     VAIGNLLFNQAIQLDLIKLFAFLSIIFIVALCRNVHYMVKHPASFLLSPLYGILHLFV
                     LQPLKLYSLCTIKNTEWGTRKKVTIFK"
     gene            4013..5257
                     /locus_tag="VGT87_001601"
     CDS             4013..5257
                     /locus_tag="VGT87_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEQ3814436.1"
                     /translation="MKIVQGVQLHLIKTKQFKTNHITFRFSGDLNQKTVAKRVLVAQM
                     LATANECYPTVRQFREKLARLYGASLSTNVLTKGLVHIVDIDITFIQDRYACNGEKIL
                     DEMIQFLKDILFSPLLSIAQYQPKVFETEKNNLINYIESDREDSFYYSSLKVKELFYC
                     NKNLQMSEYGSPELIAKETAYTSYQEFHKMLNEDQIDIFILGDFDDYRVVQLIHQFPL
                     DNRNKNLNFFHLQNSVNIIKESIEKRAVHQSILQLAYHFPSVFGQRDYYALVLLNGLL
                     GSFAHSRLFIKIREEEGLAYSIGCRFDSYTGLFEIYTGIDSQHRTKTLQLIIQELNAI
                     KMGRFSEQLIKKTRSMLLNNALLSEDYNKNIIERIYTSSYIDSSYSIKNWIKGVNEVN
                     KADIIKVANLLKLQTVYFLEGI"
     gene            5259..>6502
                     /locus_tag="VGT87_001602"
     CDS             5259..>6502
                     /locus_tag="VGT87_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEQ3814437.1"
                     /translation="MTKLVKINYPNIDEDLYYVKLENGLTVYFIKKIGFLEKTAMLTV
                     GFGSLDNKLTVDDESRDAPAGIAHFLEHKLFEDESGGDISLKFTQLGAETNAFTTFNQ
                     TSFFFSTASKFQENLELLQYFVLSANITDESVSREKKIIGQEIDMYQDDADYRAYSGI
                     LQNLFPKTSLANDIAGSKASIQKITKILLETHHTYFYQPTNMSLFIVGDIDIDETFLA
                     IQRFQTTLSYPDRKRVTVDPLHYYPVIKSSSVDMDVTTAKLVVGFRGYLTLTQHSLLT
                     YRIALKLFLSMLIGWTSKIYHTLYEDGKIDDSFDVDVEIHHNFQFVLISLDTPEPIAM
                     SNYIRQKLATIKISKEFTNEHLNLLKKEMYGDFIQSLDSIEHLTHQFSLYLSDSDKET
                     YFDIPKIIERLTLKDVVTIGKA"
BASE COUNT         2199 a         1063 c         1048 g         2192 t
ORIGIN      
        1 ttttccaaag cttttcctag tttgataatg taatttttca agtcctcttt gacctgtggg
       61 tgttctaagg catagtcgat agatgttttc atgaatccaa atttatcccc aacatcgtaa
      121 cgattgcctt taaattctcg tgcaaagaca cgctgagttt tattgagggt atcgatagca
      181 tctgtgagtt gcacttcgtt acctgctcca ggggtctgtc tttccaaaat atcaaaaatt
      241 tcaggggtta ggaggtaacg accaataata gccaaatcac taggcgcatc ttctggttgt
      301 ggtttttcaa caaaggtgtc tacactgtaa aggcccttga cagccttgcc ttgaggagcg
      361 ataaccccat agctagacac atcttcatga ggaactttca tcacagcgat agtggatgca
      421 tgcgtcttgt catagtcctc catgagttgt ttggtgagag gtttagcgga tgcatttgta
      481 atgtccatta agtcatctcc aagcatgacc acaaagggtt cattgcccac aaaggctttg
      541 gcttgtaaga cagcatctcc cagtcctctt gggtggcttt gacggataaa atgaaggtta
      601 atggcagtgg tttcatccac taatttcaac agttcatttt tccccttagc ttggaggttg
      661 tattctaatt caaagtttga atcaaaatgg tcctcgatag agcgtttagc ttttccggtc
      721 accacaagga tttcctcgat gccagatttt agcgcttctt cgacgataaa ctggatggtt
      781 ggtttatcaa cgatgggcaa catctctttg gcaagagctt tggtagcagg taaaaaacgt
      841 gttcctagac ctgcagcagg aataatggct tttctgactt tggtcatcag taactcctca
      901 ctttttataa tctatggctt agtgacattc gttttcagaa cggaattcat ttgacatcat
      961 gttacgtttt catatgattt ttcgactatt tcatagtgct caatcatacc accaactgca
     1021 gttcttttaa agtaaaaatg aatattattg ttatagatga agacatggct agtctctatt
     1081 aaaaatatct ctactgtaaa ctttattttt aacatcttgt aattcattat catagcgatt
     1141 agttacgata atatttgctt gtttcttgaa attctctaaa tcatttacaa gtacagattg
     1201 atcttcagat tcaagtttgt ttaacattgg ctcataaata attatcttaa tgtctttact
     1261 tttaagaatg tcaataacat ctttgatagc actttctcta aaattatctg agttactttt
     1321 cataattaaa cggtaaaccc cgactacttt tactggggac tcccgctctt ctaagacgtt
     1381 gataatttgc ttagcaatat aggacttgcg cacattattt gatgaaacga tagcttcaat
     1441 tagcgtttgg ggaatattat tgtaatttgc caataattgc ttcgtatctt taggtagaca
     1501 ataacctcca taaccaaatg atgggttatt ataatgcatt cctattcgat catcataaga
     1561 aattccttga ataatcatgt gactatttaa ttttctcgat tctgcgtaag tgtctaactc
     1621 attaaaataa gctaccctta acgctaaata agtattggca aatagtttta ctgcttcagc
     1681 ttctgaagct cccataataa gtactggtac attatttttt ttagctgcag actttaataa
     1741 aagtgcaaat ttttctgcgt ctgcctttac ttttggagaa tcgttttctt cacaagaaac
     1801 aataattcgg cttggatata agttgtcata taaagcttta gattctctta aaaattcagg
     1861 gctgaagata atacgatcag tttggaattt ctgtctcatt tcagtaatga aacctattgg
     1921 aattgttgat ttgatgataa gagttgcatg gctattaacg cttagtacct ctttgataac
     1981 tgtttcaaca tgctgtgtat caaaataatt aattctactg ttgtaatttg taggtgtggc
     2041 aataatgacc agttccgctt ctttataagc tgctttgcta tctaaagttg ctttaataga
     2101 taattgctta ctttttaagt aatattcaat atattcatct tgaattggtg ataagccatt
     2161 attaatctta tcaactttag agggaagaat atcaacaata gtgacttcgt tttgaagtga
     2221 taaaagaact cctagtgata atccaacata tcctgatcca gcaactgcta ttttcataaa
     2281 attactcctt ctctaactac tcgatgcata tattatttaa aaatagtgac ctttttacgt
     2341 gttccccatt ccgtattttt aatggtgcat aaagaataaa gttttagagg ctgtaagaca
     2401 aacaagtgta atattccata cagaggagat aacaaaaaac tagcaggatg tttgaccata
     2461 taatgaacat tacgacataa agcaacgata aagatgatgg ataaaaaggc aaaaagttta
     2521 ataaggtcta attgaatagc ttgattaaac aaaagattcc caattgcgac aatcaacatc
     2581 ataaacataa cgacttcgaa aatagtccat aaggcaacga tgggattaga aagaattttt
     2641 ttaacagaaa taatagattc tctaaaaaaa gatttattcc atcgattttg ttgcttaaaa
     2701 taacttttta attggaaagg tacatcagta tcacatctag ctgttgattg gtagacagtg
     2761 cgtcctaaat caatagcata atttgttaaa catcgatcat ccccaatgct aacaggtaaa
     2821 cctaggaatg tttgattttt atagcgctct aagttgggaa taatcacttc acgtcgataa
     2881 atactcaatg gtcctgagca aactaaaata ttacctgtta atgattgagc agcacgctcc
     2941 accccaaagg cattatcgta acggatatct gtaagtcgcg ttaatagatt agtttgtctg
     3001 tttctagcat tcaaatgtcc tgttgcagca taaactgtct tatcattgaa gctttttagg
     3061 agttcttcta aggcatttgg atagatataa gtatctgaat ctacggttaa aaaaacgtca
     3121 gcgtcagatc tttcaaatgc ccacgcttga gcatggcgtt ttcctttatt gacaagggaa
     3181 cggtgaacaa taacgtttcg acaaatatcc acttctctat ttacatactc ttcaattaat
     3241 tgtattgcat ctgtgtttgg actcccatca tcaacaatat aaatttctga taacggatag
     3301 gtctgtgcta acacactttt aagagtttct aataatgact cggcatcttc attataagaa
     3361 ggaattacag cagcaacttt atagtcatgt ggctttcctt taaatggctc ataaaggaaa
     3421 gataatccaa gtttaataac tagataggtt attaatatta ctccataaat tcctacagtt
     3481 gatgctccaa atagatacat atttagataa atcaagatag atatcaaaaa aataaaggat
     3541 aaaacaatta aagttttttt aaaaataggc acaattacac ctctttcttt tttaatttcc
     3601 ttgaaagaca gggacctcga tattctaata taaataaggg ataatgtcaa ctaggcatag
     3661 agatattttt aataaaaatg tatttccatt aaaaaatttc ccccaaaaaa aggaaaaatc
     3721 aggataattt atttaatata aattaaataa attatctcaa aataattgaa ttgttataaa
     3781 atattttagc aaacttttca aatagtttta tctaattttg tgaaaaaaat agatatattt
     3841 tgatttttaa aaaatatcaa gaaatatgat aactggtaaa ggatatattg tagaaaacta
     3901 tattttacta acactagaat cccgacaatt aagtcaaaaa aagttatcac tcacctaacc
     3961 tgaataaaaa tggtataatc gtaaggatca cactatttat taaggaaaca ttatgaaaat
     4021 tgttcaggga gtacaacttc atctgattaa gaccaaacag tttaaaacca atcatattac
     4081 ctttcgtttt tcaggtgatt tgaaccaaaa aacagtagca aaaagagtct tagtggcaca
     4141 gatgttagca acagctaatg agtgttatcc aactgttagg cagtttagag aaaaattagc
     4201 acgactttat ggagctagct tgtcaactaa cgtgttaaca aaagggttag ttcatattgt
     4261 ggatatcgat attactttta ttcaagatag atatgcttgc aatggggaaa aaatactaga
     4321 tgaaatgatt cagtttttaa aagatatctt gttctcaccc ctattatcca tcgcccagta
     4381 tcagcctaaa gtttttgaga ctgaaaaaaa taatttgatt aactacatcg agtcagatag
     4441 agaggattct ttttactaca gctctcttaa ggtaaaagaa ttgttttatt gtaataagaa
     4501 tctacaaatg tcagaatatg gtagcccaga actaattgca aaagagactg cctatactag
     4561 ctatcaagaa tttcataaaa tgctcaatga agatcagatt gatattttca ttttaggaga
     4621 ttttgatgat tatcgcgttg ttcagctaat tcatcaattt ccacttgata accgaaataa
     4681 aaacctaaat ttctttcatt tacaaaattc tgtcaatatt attaaagaat ctattgaaaa
     4741 aagagcagtt catcaatcta ttttacaatt agcttatcac ttcccttcag tttttggaca
     4801 acgtgactat tatgctttag ttcttttaaa tgggttgtta ggctcttttg cacattcccg
     4861 tctctttata aaaattagag aagaagaagg acttgcttat agtatagggt gtcgttttga
     4921 tagttataca ggtttatttg aaatttatac tggaattgac agtcaacatc gcactaaaac
     4981 gttacaacta atcatccaag aattaaatgc cattaaaatg ggacgattct cagaacaatt
     5041 gattaaaaag accagatcga tgttgttgaa taacgcctta ttatcagaag attataataa
     5101 aaatataatt gaaagaatat acacatcttc ttacattgat tcttcttatt ctattaaaaa
     5161 ttggattaaa ggggttaatg aagttaataa agcagatatc attaaagtag ctaatctttt
     5221 aaagcttcaa actgtctatt ttttagaagg aatataaaat gacaaaatta gtaaagataa
     5281 actatcctaa tattgacgaa gacctttact atgtaaaatt agaaaatggt ttaactgttt
     5341 attttataaa aaaaataggg tttttagaaa aaacagctat gttgacagta ggtttcggtt
     5401 ctcttgacaa taagttgaca gtcgatgacg aatcacgaga tgctccagca ggtattgcac
     5461 actttttaga acataaactc tttgaagatg aatcaggtgg agatatttca ttaaagttta
     5521 cacagctagg agcagagaca aatgccttta caactttcaa ccaaacgagt ttcttctttt
     5581 caacagctag taaatttcag gaaaatttgg aactcctcca atattttgtt ctttctgcta
     5641 atataacaga tgaatctgtt agtagagaaa aaaaaataat cgggcaagaa attgatatgt
     5701 atcaagatga tgctgactac cgtgcatata gtggaatcct tcagaactta tttccaaaaa
     5761 caagtttagc taatgatatt gctggctcaa aagcatccat tcaaaagatc acgaaaatat
     5821 tattggaaac gcatcatact tatttttacc aacctactaa tatgagtcta ttcattgtcg
     5881 gtgatattga tattgacgaa acttttttag ctattcaaag gtttcaaacc acattatcct
     5941 atccagatag aaaaagggta actgttgatc ctttacacta ctatcctgta attaaatcat
     6001 cttctgttga catggatgtt actactgcca agctggttgt tggttttcga gggtatctga
     6061 ccttaactca gcattcacta ttaacgtatc gtattgcctt gaagctattt ttatcaatgc
     6121 ttataggttg gacttctaaa atctatcata cgctatatga agatggtaaa atagacgatt
     6181 catttgatgt ggatgttgaa attcatcata attttcaatt tgtattaata tcacttgata
     6241 caccagagcc tatcgcaatg tctaactata tccgtcaaaa attagccact ataaagatat
     6301 caaaagagtt tacaaatgag cacttaaatc tattaaaaaa agaaatgtat ggtgacttta
     6361 ttcagagttt agactctata gaacatttaa ctcatcagtt cagtctatac ctgtctgatt
     6421 ctgataaaga aacatatttt gatattccaa aaattataga aagactcact ctaaaagatg
     6481 ttgttacaat tggaaaagca tt
//
LOCUS       DAUCLR010000095         6026 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.096, whole genome shotgun sequence.
ACCESSION   DAUCLR010000095 DAUCLR010000000
VERSION     DAUCLR010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.096"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            183..1454
                     /locus_tag="VGT87_001603"
     CDS             183..1454
                     /locus_tag="VGT87_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEQ3814438.1"
                     /translation="MSKRGKIKITTKTKLITASVITLVLIITGVVLWKQQQNMLTADI
                     AKEPYSTVSVTEGSIASSTLLSGTVKALSEEYIYFDANKGNDATVTVKVGDQVTQGQQ
                     LVQYNTTTAQSAYDTAVRSLNKIGRQINHLKTYGVPAVSTETNKDEATGEETTTTVQP
                     SAQQNANYKQQLQDLNDAYADAQAEVNKAQIALNDTVVISSVSGTVVEVNNDIDPSSK
                     NSQTLVHVATEGQLQVKGTLTEYDLANVKVGQSVKIKSKVYSNQEWTGKISYVSNYPT
                     ESNAGSTTPAGSTGAGSSTGAAYDYKIDIISPLNQLKQGFTVSVEVVNEAKQALVPLT
                     AVIKKDKKHYVWTYDDATGKAKKVEVTLGNADAQQQEIHKGVTVGDIVIANPDKNIKP
                     DKKLEGVISIGTNTKPEKDSQSKNKKSGVDK"
     gene            1454..2164
                     /locus_tag="VGT87_001604"
     CDS             1454..2164
                     /locus_tag="VGT87_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ3814439.1"
                     /translation="MSHEKKQLMQLSNIVKSYQNGDQVLKVLKGINLTVYEGEFLAIM
                     GPSGSGKSTLMNIIGLLDRPTSGDYTLHNTKIEIFNDRELAKVRNDEIGFVFQQFFLL
                     AKLTALQNVELPLIYAGVNVSKRREQAKQFLEKVGLGRRIKHLPSELSGGQKQRVAIA
                     RALVNDPSIILADEPTGALDTKTGQQIMELLTELNKEGKTIIMVTHEPEIADFATRKI
                     IIRDGDITTDTTASVVID"
     gene            2176..3396
                     /locus_tag="VGT87_001605"
     CDS             2176..3396
                     /locus_tag="VGT87_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ3814440.1"
                     /translation="MENWKFALSSIWGHKMRSILTMLGIIIGVAAVVIIMGLGNAMKN
                     SVTSTFSSKQKDIQLYFQEKGEEEDLYAGLHTHENNHEVKPEWLEQIVKDIDGIDSYY
                     FTNSATSTISYEKKKVDNASIIGVSKDYFNIKNYDIVAGRTLTDNDYSNFSRIILLDT
                     VLADDLFGEGNYKSALNKVVSLSDKDYLVIGVYKTDQTPVSFDGLSGGAVMANTQVAS
                     EFGTKEIGSIYIHVNDIQNSMNLGNQAADMLTNISHIKDGQYAVPDNSKIVEEINSQF
                     SIMTTVIGSIAAISLLVGGIGVMNIMLVSVTERTREIGLRKALGATRLKILSQFLIES
                     VVLTVLGGLIGLLLAQLSVGALGNAMTLKGACISLDVALIAVLFSASIGVFFGMLPAN
                     KASKLDPIEALRYE"
     gene            3650..5383
                     /locus_tag="VGT87_001606"
     CDS             3650..5383
                     /locus_tag="VGT87_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HEQ3814441.1"
                     /translation="MTFLSDLISLMTKIRLSWVIKAGIFQLLFVTIANIVLSEFFYFI
                     LDVTGQYHLDKDNVVTFLKNPIALALLGAYLFLLAAFIHLEFFALYRIIADQEISFYL
                     FRKQFSYYLRGLWKTFSGYQLLLFLLYILLTIPVLHIGLSSVITQKLYLPEFIVGELS
                     KITSTKYLLYGSLILVFYLNLRLVYFLPLIAINHRTVAQAWRESWQKTKKKHVLLWMK
                     LFAINGLTLVVLSLAISMILIFVDMFNPKGNNIIVQLGALTFTWELIFFTTIFFKLCS
                     AMILKEAIEPQKQYDEPRRSNKAYVVIFIVVTVGFAYQSLERLTFFDISHSKTVIAHR
                     GLVSAGVENSLEALEGAKKAGSDYVELDLILTKDNHFVVSHDNRLKRLAGVNKTIRNL
                     TLKEVEHLTSHQGHFSGRFVSFDTFYQKAKKLNMPLLIELKPIGTEPGNYVDLFLETY
                     HRLGISKDNKVMSLDLEVIEAIKKKNPSITTGYIIPIQFGFFGDEFVDFYVIEDFSYR
                     SYLSSQAFWNNKEIYVWTINDPKRIEHYLLKPIQGIITDQPALTNQLIKDLKQDNSYF
                     SRLVRIISSLY"
     gene            5510..5782
                     /gene="rpsP"
                     /locus_tag="VGT87_001607"
     CDS             5510..5782
                     /gene="rpsP"
                     /locus_tag="VGT87_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEQ3814442.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRAPRDGRFIETVGTYNPLV
                     AENQITIKEDRVLEWLSKGAQPSDTVRNILSKAGVMAKFHDQKFSK"
     gene            5792..>6026
                     /locus_tag="VGT87_001608"
     CDS             5792..>6026
                     /locus_tag="VGT87_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEQ3814443.1"
                     /translation="MDTIENLIIAIVKPLISQPDNLTIKIEDTPDFLEYHLDLDAQDI
                     GRVIGKKGRTITAIRSIVYSVPTLGKKVRLVIDE"
BASE COUNT         2035 a          908 c         1144 g         1939 t
ORIGIN      
        1 aacttaaaaa tgattttttt agattataat gacaaaagtt tcagtaagct attaatttgt
       61 catagcatat aagtcattat tttgtggctt gttttcgact aaggactaag aatttcttga
      121 caacaatata aaaaggtcat atacttgtct tatacaaata gtacaaaaag gggaggatag
      181 aaatgtctaa aagaggaaaa attaaaataa caacgaaaac aaagcttatt acagctagtg
      241 ttataacgct agtattaatt ataactggag tagtcttgtg gaaacaacaa caaaatatgc
      301 taacagctga tatcgctaaa gaaccttact ctactgttag tgtaactgaa gggagtattg
      361 cttcttcgac tttactatca ggtactgtaa aggctttatc agaggaatat atttattttg
      421 atgctaataa aggaaatgat gcaactgtta cagttaaagt aggtgatcag gtaacgcagg
      481 gccagcaatt agttcaatat aatacaacaa cagctcagtc agcttatgat actgctgtta
      541 ggagtcttaa caagattggc cgacaaatta atcatcttaa aacatacgga gttcctgctg
      601 ttagtacaga aactaataaa gatgaagcta ccggtgaaga gacgacaaca acagttcaac
      661 catcagctca gcaaaatgct aattataaac agcagctgca agatttaaat gatgcttatg
      721 cagatgcaca agcagaagta aataaagcgc agatagcgtt aaatgataca gtagttatca
      781 gtagtgtctc tggaactgtt gtggaagtaa ataatgatat tgatccttct tcaaagaaca
      841 gtcaaacact tgttcacgta gcaaccgaag gacagcttca ggtgaaagga acattgacag
      901 agtatgattt agcaaacgtt aaggttggtc aatctgtaaa aattaagtct aaagtttatt
      961 ctaatcaaga atggactgga aaaatatcat atgtttcaaa ctatccaact gagtctaatg
     1021 caggttcaac aacgccagca ggtagcactg gagcgggaag ttctacagga gctgcctatg
     1081 attacaagat tgatattata agtcctctca accagcttaa acaaggcttc actgtttctg
     1141 ttgaggttgt taatgaagca aaacaggcct tagttccttt aacggctgtt attaagaaag
     1201 ataaaaaaca ctatgtttgg acttatgatg atgctactgg caaagccaaa aaagtagagg
     1261 tgacacttgg aaacgcagat gcacaacaac aagaaattca taaaggagta actgttggtg
     1321 acattgttat tgccaatcca gataaaaata tcaaaccgga taaaaaacta gaaggggtta
     1381 tttcaatagg taccaacaca aaaccggaaa aagattctca atcaaagaat aaaaaatcag
     1441 gggtggataa atagtgtcgc atgaaaaaaa acaattaatg caattatcaa atattgtgaa
     1501 gagctatcaa aatggggatc aagttcttaa agttttgaaa ggtattaatt taacagtata
     1561 tgagggtgaa tttttagcta ttatgggtcc gtctggctcg ggaaaatcaa cactaatgaa
     1621 tattattggt ttattagatc ggccaacatc cggagattac acattacata atactaaaat
     1681 agaaatcttt aatgatcgtg aattagcaaa agtacgaaat gatgaaatcg gctttgtctt
     1741 tcaacaattc tttctgttag ctaaattaac tgcattacaa aatgttgagt tgcctcttat
     1801 ttatgctggt gtaaacgtct caaaaagacg tgaacaggct aagcaatttc ttgaaaaagt
     1861 aggacttgga cgacgtatca aacatctacc gtcagaactg tcaggaggtc aaaagcaaag
     1921 ggtggctatc gcacgagctt tagtaaatga cccatctatt attttagcgg atgaaccaac
     1981 aggtgcacta gatactaaaa caggtcagca aattatggaa ctcttgacag agttaaacaa
     2041 agaaggtaaa acaattatta tggttactca tgaaccagaa attgctgatt ttgcaactcg
     2101 gaaaattatt attcgtgatg gtgatatcac aacggatact acagctagtg ttgttattga
     2161 ctaggaggaa aaataatgga aaattggaaa ttcgctctta gctcaatttg ggggcataag
     2221 atgcgctcaa tcctgacaat gttgggaatt atcatcggtg tagcagcagt tgtgattatt
     2281 atggggttgg gaaatgctat gaaaaatagt gtaacaagca cattttctag caagcaaaaa
     2341 gatattcaac tctattttca agaaaaagga gaagaagaag acctgtatgc cggattgcac
     2401 acgcatgaaa ataatcacga agtgaagcca gagtggttag aacaaattgt taaagatatc
     2461 gatggtattg attcttatta ctttaccaat tctgctactt ctactatttc gtatgaaaaa
     2521 aagaaagtag acaatgcttc gatcataggg gtaagtaaag attattttaa tataaaaaac
     2581 tatgatatcg tagctggtcg tacactaaca gataatgatt attctaattt ttcgcgtatc
     2641 attcttttag atacagtatt agccgatgac ctatttggcg aaggtaatta taagtcagct
     2701 ttaaataaag tggtaagttt atctgataaa gattatttag ttattggtgt ttataaaact
     2761 gatcagacgc cagtttcttt tgatggatta tcaggcggcg ctgtcatggc aaatactcag
     2821 gtagcttcag agtttggcac caaagaaata ggatctatct acattcatgt gaacgatatt
     2881 caaaacagta tgaacttagg aaatcaagca gctgatatgc taaccaacat atcgcatatt
     2941 aaagatggac agtacgctgt tcctgataat agcaaaatcg ttgaagaaat caatagtcag
     3001 ttcagtatca tgacaacagt cataggttca attgcagcga tctctctttt agtgggagga
     3061 attggtgtta tgaatattat gttggtatct gtcacagagc gaactaggga aattggttta
     3121 cgtaaagcac ttggtgcaac acgcctgaaa attttatcac aatttttgat tgaatccgtt
     3181 gttctaacag ttcttggagg cttgatagga ttattattag ctcaattaag tgtaggggct
     3241 ttaggaaatg cgatgacatt aaaaggagct tgtatttctc ttgatgtggc cttaatagca
     3301 gttttatttt ctgcctcaat aggtgtcttt tttggaatgt tgccagctaa taaagctagt
     3361 aagttagatc caattgaggc attacgttat gaatagaata gttcgtatca attaatctat
     3421 agacgctgac tagtttaacg tctgattata tctatttttt gtattagcac agtagttata
     3481 tcattgaagt gttagtgcat tttttgaggt gataatatct agcaaagcta tcattggtat
     3541 gatgatgtgg atagcgtata agtgatacat ctcttatttc tagctgaact tcggttcggc
     3601 taggaagttg tcaggcaatg agttgtttaa aataaaggag attagttcaa tgacattttt
     3661 atctgatttg atatcattaa tgacaaaaat cagattatcc tgggtaataa aggcgggtat
     3721 ttttcaatta ttatttgtaa cgattgctaa tattgtctta tcagaatttt tctattttat
     3781 attagacgtt actggtcaat atcatttaga taaagacaat gttgtgactt ttttaaaaaa
     3841 tcctatagca cttgctttat taggtgccta tttattttta ttagctgctt ttattcacct
     3901 tgagtttttt gctctatatc gaattattgc ggatcaagaa attagtttct atctttttag
     3961 aaaacagttt tcttattacc taagggggct ttggaaaaca ttttctggtt accaattatt
     4021 actttttttg ctttatatcc tactgactat tccagtctta catattggtt tatcttctgt
     4081 gattactcaa aagctttatc ttccagaatt tattgttggg gaattatcaa agataactag
     4141 cacaaagtac ttgctttatg gcagtcttat tcttgtgttt taccttaacc taagattagt
     4201 atatttttta ccattgatag caatcaacca tcgtacggtt gctcaagcat ggagagagag
     4261 ttggcaaaag actaaaaaga aacatgtatt gttatggatg aaactttttg caatcaatgg
     4321 tcttacgctt gtagtcttat cgctagctat ttccatgatt cttatttttg ttgatatgtt
     4381 taatcctaag gggaataata ttattgttca gctgggagct ttgaccttta catgggagct
     4441 catttttttt actactattt tttttaaact ctgttcagca atgattttaa aagaggcaat
     4501 tgaaccacaa aagcaatatg atgagccaag aagaagtaat aaggcatatg ttgtaatctt
     4561 tatcgtggtg acagtaggtt ttgcttatca atctcttgaa cgtttaactt tttttgacat
     4621 atctcactct aagacagtta tcgcgcatag aggacttgta tcagcaggtg tagaaaattc
     4681 tctggaagcc cttgaaggtg ctaagaaagc aggaagtgat tatgtagaac tggatctaat
     4741 cttgactaag gataatcact ttgtggtgtc tcatgataat cgattgaagc gtttagctgg
     4801 agtaaataag acgattcgca acttaacctt aaaagaagtt gaacatctaa cgagtcatca
     4861 aggacatttt tcagggcgtt ttgtttcttt tgacactttt tatcaaaagg ctaagaagtt
     4921 gaatatgcca ttacttattg aactcaagcc aattggtaca gaacctggaa attatgtcga
     4981 tttgttttta gaaacttatc atcgacttgg tataagcaaa gataataaag tcatgtcttt
     5041 agatttagaa gtaatagaag ctatcaagaa aaaaaatcca tcaattacga ctggttatat
     5101 cataccaatt caatttggct tttttggaga tgaatttgtt gatttctatg tcattgaaga
     5161 cttttcttat cggtcttatt tgtcgtccca agctttttgg aataataaag aaatttacgt
     5221 ttggactatt aatgatccca agcgcataga gcattatctc ctaaagccta ttcagggaat
     5281 tattacagat caaccagctt taactaatca attgattaaa gacttaaaac aagataattc
     5341 ttattttagt cgattagtca gaattattag tagtctttat taaaggttta aagaaagtgt
     5401 caagaaaaaa acttgacact ttctttaaac tgagttatac tgtttaacat aggaatttag
     5461 cgtagctgta tttcttatac attactaaaa gaaaagagac tatttaaaaa tggcagtaaa
     5521 aatccgttta actcgtatgg gttctaagaa aaaacctttc taccgtatta atgttgcaga
     5581 ttcacgtgca ccacgtgatg gtcgttttat cgaaacagtt ggaacataca acccattagt
     5641 ggctgaaaat caaattacta ttaaagagga tcgtgttctt gagtggttat ctaaaggagc
     5701 gcagccttct gataccgttc gtaacatctt gtctaaagct ggtgttatgg ctaagttcca
     5761 cgaccaaaaa ttctctaaat aataatagcc tatggatacc attgaaaatc ttattatcgc
     5821 tattgtgaaa cctttgattt cacaaccaga taatcttacc attaaaattg aagatactcc
     5881 tgacttttta gagtatcatc ttgacttgga tgctcaagac atcggtcgtg ttatcggcaa
     5941 aaaaggtcga actattactg cgataagatc gattgtctac tcggttccaa cactaggaaa
     6001 aaaagttcgc ttagttattg atgaga
//
LOCUS       DAUCLR010000096         5778 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.097, whole genome shotgun sequence.
ACCESSION   DAUCLR010000096 DAUCLR010000000
VERSION     DAUCLR010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.097"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(1..1046)
                     /locus_tag="VGT87_001609"
                     /pseudo
     CDS             complement(1..1046)
                     /locus_tag="VGT87_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632209.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            1282..2013
                     /gene="aphA"
                     /locus_tag="VGT87_001610"
     CDS             1282..2013
                     /gene="aphA"
                     /locus_tag="VGT87_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HEQ3814444.1"
                     /translation="MKKEFTSILFTVSFCGIIALPVEASGPKVPYTQEGITAISNQAT
                     VKLISIADIASSLEGQKPITVSFDIDDTLLFTSQYFQYGKEYITPGSFDFLHKQKFWD
                     LVAKRGDQDSIPKEYAKQLIAMHQKRGDKIVFITGRTRGSMYKKGEIDKTAKSLAKDF
                     KLDKPIAINYTGDKAVKPYQYDKTYYIKKNGSQIHYGDSDEDINAAKEAGARPIRILR
                     APNSTNLPLPKAGGYGEEVLENSAY"
     gene            2191..3723
                     /locus_tag="VGT87_001611"
     CDS             2191..3723
                     /locus_tag="VGT87_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEQ3814445.1"
                     /translation="MENHKNEFTFSNKSIIAYVWRGVMVGIIAGVIVSLFRLLIEVTA
                     DWVIEWYRYAHINSLLLLPILSVSLLAVLFVGFLVKSDSDIKGSGIPHVEGELKGLMS
                     PDWWSVLWKKFLGGIMAISMGFMLGREGPSIQLGAMSAKGLAKFLKSSRLEKRVLIAS
                     GAAAGLSAAFNAPIAGLLFVVEEIYHHFSRLIWITALVASLVANFISLNIFGLKPILA
                     MSEAMPFLGLNQYWLLLLLGLFLGCLGYLYEIVILNFNKLYVILGSWLHLPAYFYGII
                     MVFLILPIGYYLPQLLGGGHGLILSLSNQQLPLMTIFFYFIIRFIVSMFSYGSGLPGG
                     IFLPILTLGALAGLLFGQIASQLGLLNQSFLSLFLILGMAGYFAAISKAPLTGMILVT
                     EMVGDLKPLMAIAVVTFVSYLVMDLLNGQPIYEAMLDKMAMKHPTNLVEPTLIELTVG
                     DKIAGKYVKELKLPENVLITTQIHHQKSQVVSGNTRLLSGATIFLVVNEADTGFVREV
                     LM"
     gene            3886..4500
                     /locus_tag="VGT87_001612"
     CDS             3886..4500
                     /locus_tag="VGT87_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HEQ3814446.1"
                     /translation="MLEIVSEELRHYQEQKLIEYRNKNQLAPKGGIVFAGDSLIEFFP
                     LKKAFGSCLPIINRGIAGIDSQWLLRHFSVQITDLEPKHIFLLIGCNDIGLGYDKCHI
                     VKTIVELISQIRSHCVYSQIYLLSLLPVSNNPRYQKTVKIRTNAMIDAINKDLAMIPT
                     IEFINLNTCLKDEKGGLSDENTLDGLHLNFPAYAKLAEIIQSYI"
     gene            4659..>5778
                     /locus_tag="VGT87_001613"
     CDS             4659..>5778
                     /locus_tag="VGT87_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEQ3814447.1"
                     /translation="MIIKGVTMKFEKKQVFYLVLTFILCYGILANWRNGTAIVTTIYK
                     TSLPFFYGAAGAYIVNIVMSAYEKVYVYIFKDWSHVLKVKRGICLLLAYLTFFILITW
                     IISIVIPDLITSISTLTKFDTITIQEVVNNLEHNKLLARTIQYIGGDGKLTETIANYS
                     QQLLKQFLTVLTNILTSVTVIASAIINLFISFVFSLYVLASKEDLCRQGNTLVDTYTG
                     KYAKRIHYLLELLHQRFHGFFVSQTLEAMILGSLTASGMFILRLPFAGTIGVLVAFTA
                     LIPVIGASIGAAIGFILIMTQSMSQAIIFIIFLIILQQIEGNFIYPKVVGGSIGLPAM
                     WVLMAITIGASLKGIVGMIIAVPLAATLYQVIKDNIQKR"
BASE COUNT         1772 a          910 c         1102 g         1994 t
ORIGIN      
        1 aatatcatta tcaataataa ctttcaaggc gttatttgca ccagcatgtt taaaggtttc
       61 ataagctttt tcaacttctg aaagtttaaa ataatgtgta ataagtcttg ttgcgtcaat
      121 ctttccagtt ttcaagacat ttagtagcat ttcagttgta ttggcattaa caagtccagt
      181 gtttaaggtg atatttttaa tccataattc atccaggttg aaatcaactg gtttcccatg
      241 aaccccaacg ttagcaatat gcccacctac tgatataatt ttttggcaaa tatcaaatgt
      301 cgcgggataa ccaacacact ccatggaaat atcgacacca cgtccattgg ttatatcgtc
      361 aatgatagcc ttgacttctt cactacttcc tgaacaaatc gtgtgtgtgg ctccaaacgt
      421 tttagctgct tcaaggcgat tttgagataa gtcgaccata ataatattgg ctggagagaa
      481 aagttgaacg gttagtaggg ctgcgagtcc tactggaccg gcaccaataa tacaaacatt
      541 atctccaggc ttgacatgtg atggcaagac accaatttca tatgaagttg gtaaaatatc
      601 agaaagcatg accagtgcct catcatcaat cgtatcagga gcatgataga gactaccatc
      661 tgcatgtggg atatggacat attctgcttg tgttccatta attaaatggc caagaatcca
      721 accgccatct tcacaatgtg acgaaaggcc tcgtttacaa taataacagg tatgacatga
      781 tgtgacgcaa gaaataatca ctttatctcc gattttaaag ttagtgaccg catcaccgat
      841 ctccttaaca attccaattc cttcatgacc aaggattgtt ccttctttac atgcgggaac
      901 atccccacct aagatatgca aatcagttcc acaaatagag tggtttttac caactggacg
      961 atagcatctg taggctttat aatgactggt tttggtttgt ctatgagctg tagatttccg
     1021 gttgaaaggt aagttgctgc tttcatattt aactctttat aaggttgaga ggtaatcaac
     1081 caattcattt ttacacagat attataatag tttaagaaag ggttttcaat attttttaaa
     1141 tcgattatga taatttttta gatcatcttt gttgaataac ttcatcctta ttcagaactt
     1201 aaagggcact attataagaa tttgaaaccg ctttacttaa atcgtgtatt tttgtacaat
     1261 aatgattaag gaggttttta gatgaaaaaa gaatttacca gtatcctgtt tacagttagt
     1321 ttttgtggaa tcatagcatt gcctgtcgaa gctagtggcc ctaaagttcc ttatacacaa
     1381 gaaggtataa cggccatttc aaatcaggca acagtaaaat tgatttctat tgctgatatc
     1441 gcgagcagtt tagaaggaca aaaacctata acggttagtt ttgatatcga tgatactttg
     1501 ttatttacta gccaatattt tcaatacggc aaagaatata taactccagg ctcatttgat
     1561 tttttgcata agcaaaaatt ttgggacttg gtcgctaaac ggggagatca ggattctatt
     1621 ccaaaagagt atgcaaagca actaatcgca atgcatcaaa aacgaggaga taaaattgtc
     1681 tttattactg gccgaactcg aggatcgatg tataaaaaag gtgagattga taaaacagct
     1741 aagtcactgg ctaaagattt taagttagac aagccaatag ctatcaatta tacaggagat
     1801 aaagctgtga agccgtatca gtatgataaa acatattata taaagaaaaa cggctcacaa
     1861 attcattacg gagatagtga tgaagatata aatgctgcca aagaggcagg tgcaagacct
     1921 attcgcattt taagagctcc taattcaact aatcttcctt tgccaaaagc tggaggatac
     1981 ggagaagagg ttttagaaaa ttcggcttat tagtcaaata tttgtgtaag aaaggaagta
     2041 acagtaattt ctttcatcac cccactgagt aaccaatagc ccagtgggtt ttttgggtta
     2101 cagaaatcag ttctaaaaaa gaatcactgg taacttaagg ttgtctttga tacaatggta
     2161 acagtgtatt taaaagaaag agaggataac atggaaaacc ataaaaatga attcaccttt
     2221 tccaacaaat caattattgc ctatgtttgg cgtggtgtta tggttggtat tattgctggt
     2281 gttattgtta gtttattccg gttgttaatt gaagtgacag ctgactgggt gattgaatgg
     2341 taccgatacg ctcatatcaa ttcgctgttg ctacttccta tactaagtgt cagtttatta
     2401 gctgttcttt ttgtaggttt tttagtaaag tctgattctg atataaaagg atcagggatc
     2461 ccccatgtcg aaggagaact aaagggacta atgtccccag attggtggag tgtcctttgg
     2521 aaaaaatttt taggaggaat catggctatt tctatgggat ttatgctcgg tcgtgagggt
     2581 ccttcgattc agttaggtgc tatgtcagcc aaaggattag ctaagttttt aaaatcgagt
     2641 cgtttggaaa agcgagtgct aattgccagc ggagcagcag cagggctatc agcagctttt
     2701 aatgctccaa tagcaggcct cttgtttgtt gttgaagaaa tttaccatca tttttcgcgt
     2761 ttaatttgga ttacagcgct agtggctagt ttagtggcta attttatttc tctgaatatt
     2821 tttggattga aaccaatctt agcaatgtca gaggcgatgc cttttttagg actcaatcaa
     2881 tattggttgt tgcttttact tggattattt ctaggctgct taggctattt atatgagata
     2941 gttatcctta attttaataa actttatgtt attttaggga gttggttaca tctgccagct
     3001 tatttttacg gtattattat ggtttttcta attttaccga ttggctatta tttaccacag
     3061 ttacttggag gagggcatgg tttaatactt tctttatcca atcaacaatt gcctttgatg
     3121 actatcttct tttattttat tattcgattt attgttagta tgtttagtta tggaagtggc
     3181 ttacctggag gtattttttt acctatttta actttaggtg ctttggcagg cctattattt
     3241 gggcaaatag cttctcaatt aggtttattg aaccaatctt ttctttctct ttttttgata
     3301 ttagggatgg cagggtattt tgcggcaatt tcaaaagcac cactgacagg catgattttg
     3361 gtaactgaaa tggtaggtga tttgaaaccc ttaatggcca tagctgtagt tacttttgtc
     3421 tcctatttag tgatggattt gttaaatgga cagccaattt acgaagctat gctagataag
     3481 atggcaatga agcatccaac taatcttgtt gaaccaacat taattgaatt gactgttggt
     3541 gataagattg cagggaaata tgttaaagag ttaaagcttc cagaaaatgt cttaattaca
     3601 acccaaattc accatcaaaa atctcaagtt gtgtcaggta acacgcgcct tttgtcagga
     3661 gcaactatct ttttggtcgt taatgaagct gatacaggtt ttgtccgtga ggtattgatg
     3721 taggtattaa tttatcacaa ttcggaaagt tcgagcttgt cttttagact aagtcttcga
     3781 acgttttact gctagagttt catctgaacc aaagtgaaac gcttttgatg aagtcaagta
     3841 ggctttttgt tatactaaca ataaagataa gctaaggagg ctgagatgtt agaaattgtt
     3901 tcagaagagc tacgtcacta tcaagaacaa aaattgattg agtaccgtaa caaaaatcag
     3961 ttagccccaa aaggaggcat tgtatttgca ggtgattctc tcattgaatt ttttccttta
     4021 aaaaaagctt ttggttcatg tttgcctatt attaatcgag gtattgctgg gatagatagt
     4081 cagtggttat tgcgccattt ttctgtccaa attactgatt tagagcctaa acatattttt
     4141 ttattaattg gttgtaatga tatcgggctt ggatatgata aatgtcatat tgtcaagact
     4201 attgtagagc ttattagcca gattcgtagt cattgtgtct acagtcagat ttatctctta
     4261 tctttattac cagtttccaa caatccccga tatcagaaaa ctgtcaaaat aagaacaaat
     4321 gctatgattg atgctattaa taaagattta gcaatgattc caactataga atttattaat
     4381 cttaatactt gtcttaaaga cgaaaaaggt ggtttatcag atgagaatac tctagatggt
     4441 ttacatctta attttccagc ttatgctaaa ctagctgaaa ttattcaatc ttatatctag
     4501 ccaataaatg tactaatagc gatgaggcaa gaagtcagat agcagatggt tcatcaatca
     4561 attaagaaaa aagttatcaa gataccatta aaaatagttg ctcaaagttc tttcaaatca
     4621 aaagattatg atatcgctat gttgagtttt agtgtataat gataattaaa ggagttacca
     4681 tgaaatttga aaaaaaacaa gtcttctatt tagtccttac gtttatctta tgctatggga
     4741 tcctagcaaa ctggcgaaat ggaacggcta tcgtcacgac tatttacaaa acaagccttc
     4801 ctttttttta tggagcagca ggtgcctata ttgttaatat tgtaatgagc gcttacgaaa
     4861 aggtatatgt ttatatcttt aaagactggt cacatgtctt aaaagtaaaa cggggtattt
     4921 gtttactgtt agcgtatttg acatttttta ttttaattac ttggattatt tccattgtca
     4981 ttccagactt aattacaagt attagtactc taacaaaatt tgatactata acgatacaag
     5041 aagttgttaa caatcttgag cacaataaac tgttagcacg tactattcag tatattggtg
     5101 gcgatggcaa acttacagag acgattgcta actatagtca acagttatta aagcaatttc
     5161 ttactgtttt gacaaatatt ttaacatctg tcacggttat tgcttctgca attattaatc
     5221 tttttattag ttttgttttt tcgttatacg ttttagcaag taaagaagat ctttgtcgtc
     5281 agggaaatac tttagtagat acttatactg gtaagtacgc taaacgcatt cattacttgt
     5341 tagagttgtt gcatcagcgt tttcacggat tttttgttag tcagacctta gaagctatga
     5401 ttttaggttc gctgactgct agtggcatgt ttatattaag attaccattt gcagggacta
     5461 ttggcgtttt agtagctttt acagcattga ttccagttat aggtgcttca attggagcag
     5521 ctattggatt tattttaatt atgactcagt ccatgtcaca ggctattatt tttatcattt
     5581 ttttaattat tttgcagcag attgaaggta attttattta tccgaaagta gttggtggat
     5641 cgattggatt accggctatg tgggtattaa tggcaattac aataggcgct tctttaaagg
     5701 gaatagttgg tatgattatt gcagttcctt tagcagcgac actttatcaa gtgattaaag
     5761 ataatattca aaaaagac
//
LOCUS       DAUCLR010000097         5767 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.098, whole genome shotgun sequence.
ACCESSION   DAUCLR010000097 DAUCLR010000000
VERSION     DAUCLR010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5767
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.098"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            175..4572
                     /locus_tag="VGT87_001614"
     CDS             175..4572
                     /locus_tag="VGT87_001614"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEQ3814448.1"
                     /translation="MSDLFAKLMDQIEMPLDMRRSSAFSSADIIEVKVHSVSRLWEFH
                     FAFAAVLPIATYRELHDRLIRTFEAADIKVTFDIQAAQVDYSDDLLQAYYQEAFEHAP
                     CNSASFKSSFSKLKVTYENDKLIIAAPRFVNNDHFRKNHLPNLVKQLEAFGFGTLTID
                     MVSDQEMTEHLTKDFVSSRQALVEKAVQDNLEAQKSLEAMMPPVEEATSAPKFDYKER
                     AAKRQAGFEKATITPMIEIETEENRIVFEGMVFDVERKTTRTGRHIINFKMTDYTSSF
                     ALQKWAKDDEELRKFDMIAKGAWLRVQGNIETNPFTKSLTMNVQQVKEIVHHERKDLM
                     PEGQKRVELHAHTNMSTMDALPTVESLIDTAAKWGHKAVAITDHANVQSFPHGYHRAR
                     KAGIKAIFGLEANIVEDKVPISYDPVDMDLHEATYVVFDVETTGLSAMNNDLIQIAAS
                     KMFKGNIVEQFDEFIDPGHPLSAFTTELTGITDKHLQGAKPLVTVLKAFQDFCKDSIL
                     VAHNASFDVGFMNANYERHDLPKITQPVIDTLEFARNLYPEYKRHGLGPLTKRFQVSL
                     DHHHMANYDAEATGRLLFIFLRDAREKHGIKNLLQLNTDLVAEDSYKKARIKHATIYV
                     QNQVGLKNMFKLVSLSNIKYFEGVPRIPRTVLDAHREGLLLGTACSDGEVFDAVLTKG
                     IDAAVDLARYYDFIEIMPPAIYQPLVVRELIKDQAGIEQVIRDLIEVGKRAKKPVLAT
                     GNVHYLEPEEEIYREIIVRSLGQGAMINRTIGRGGGAQPAPLPKAHFRTTNEMLDEFA
                     FLGKDLAYQVVVQNTQDFADRIEEVEVVKGDLYTPYIDKAEETVAELTYQKAFEIYGN
                     PLPDIIDLRIEKELTSILGNGFAVIYLASQMLVNRSNERGYLVGSRGSVGSSFVATMI
                     GITEVNPMPPHYVCPSCQHSEFITDGSVGSGYDLPNKPCPKCGTPYQKDGQDIPFETF
                     LGFDGDKVPDIDLNFSGDDQPSAHLDVRDIFGDEYAFRAGTVGTVAEKTAYGFVKGYE
                     RDYGKFYRDAEVDRLAAGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAS
                     WQTTHFNFHDIDENVLKLDILGHDDPTMIRKLQDLSGIDPITIPADDPGVMALFSGTE
                     ILGVTPEQIGTPTGMLGIPEFGTNFVRGMVNETHPTTFAELLQLSGLSHGTDVWLGNA
                     QDLIKEGIATLKTVIGCRDDIMVYLMHAGLQPKMAFTIMERVRKGLWLKISEEERNGY
                     IDAMRENNVPDWYIESCGKIKYMFPKAHAAAYVLMALRVAYFKVHYPIMYYCAYFSIR
                     AKAFELKTMSGGLDAVKARMEDITIKRKNNEATNVENDLFTTLEIVNEMLERGFKFGK
                     LDLYKSDAIEFQIKGDTLIPPFIALEGLGENVAKQIVKARQEGEFLSKMELRKRGGAS
                     STLVEKMDEMGILGNMPEDNQLSLFDDFF"
     gene            4682..5110
                     /locus_tag="VGT87_001615"
     CDS             4682..5110
                     /locus_tag="VGT87_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEQ3814449.1"
                     /translation="MSHLDKNTALKAMVVFRKAQRTLDAFGADIFKKADLTATQFSVL
                     EVLYTKGCMRINHLIDSLLATSGNMTVVLNNMERNGWISKCKDKTDKRAYVVTLTDKG
                     TRLIEAVLPKHVARVEEAFAVLTEKEQLCLIELLKKFKQL"
     gene            5240..>5767
                     /locus_tag="VGT87_001616"
     CDS             5240..>5767
                     /locus_tag="VGT87_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEQ3814450.1"
                     /translation="MKHILFIVGSLREGSFNHQLAAQAQKALEHQAVVSYLNWKDVPV
                     LNQDIEANAPLPVVDARQAVQSADAIWIFTPVYNFSIPGSVKNLLDWLSRALDLSDPT
                     GPSAIGGKVVTVSSVANGGHDQVFDQFKALLPFIRTSVAGEFTKATVNPDAWGTGRLE
                     ISKETKANLLSQAEAL"
BASE COUNT         1672 a         1118 c         1330 g         1647 t
ORIGIN      
        1 accaaaaacg cttggcgata aaagattgac aaaaaaatga tctcagctga gctatcactt
       61 ggactaaatt agccaaagaa cgtcagaaga aagccctgct tttctttcca tctaaaactc
      121 gaaaatcatc ttgaattgtg ataaaataaa gcctagtaaa atcaggagaa aattatgtca
      181 gatttattcg ctaaattgat ggaccagata gaaatgccac ttgacatgag acgttcaagt
      241 gccttttcat ctgctgatat tatcgaggta aaggtgcatt cggtgtcacg cttgtgggaa
      301 tttcattttg ctttcgcggc ggttttaccg attgcaactt atcgtgaatt gcatgatcgt
      361 ttgataagaa cttttgaggc ggctgacatt aaggtaacct ttgatatcca agctgctcag
      421 gtggattatt cagatgatct gcttcaagct tattaccaag aagcttttga gcatgcaccg
      481 tgtaatagtg ctagttttaa atcttctttc tcaaagctca aagtgactta tgagaatgac
      541 aaactcatta ttgcagcgcc gcgttttgtt aataatgacc attttagaaa aaatcatttg
      601 cctaatttgg tcaagcaatt agaagccttt ggctttggca ccttaaccat tgatatggtg
      661 tcagatcagg agatgactga gcatttgacc aaggattttg tttccagtcg tcaggctctt
      721 gtggaaaagg ctgtgcagga taatttggaa gcccaaaaat cccttgaggc catgatgcca
      781 ccagttgagg aagccacatc tgctcctaag tttgactaca aggaacgagc agctaagcgt
      841 caggcagggt ttgaaaaagc gaccatcaca ccaatgattg agattgagac cgaagaaaac
      901 cggattgtct ttgaggggat ggtttttgat gtggagcgta aaacgactag aacaggtcgt
      961 catatcatta actttaaaat gacagactat acctcctcgt ttgctctcca aaaatgggcc
     1021 aaagacgatg aggagctccg taaatttgat atgattgcta agggggcttg gttacgggta
     1081 caagggaata ttgagaccaa tccttttacg aagagtctca ccatgaatgt ccagcaggtc
     1141 aaagaaattg tacatcatga gcgcaaagac ctgatgccag aagggcaaaa gcgggtcgaa
     1201 cttcatgccc acaccaatat gtctaccatg gatgccttac cgacagtaga aagcttgatt
     1261 gatacggcag ccaagtgggg acacaaggcg gttgctatca ccgaccatgc taatgtgcaa
     1321 agttttcctc atggctacca tagggctcgc aaagctggaa ttaaggctat ttttggccta
     1381 gaagccaata ttgttgagga caaggtgcct atttcttatg atcctgttga tatggatttg
     1441 cacgaagcta cctatgtggt ctttgacgtg gaaaccacag gtctatctgc tatgaataat
     1501 gacctgattc agattgcggc ttccaaaatg tttaaaggaa atattgtaga gcagtttgat
     1561 gaattcattg atcctgggca tcctctttct gcctttacca ccgaattgac agggattacc
     1621 gataagcatt tgcagggcgc caagccattg gttaccgtcc taaaagcttt tcaggacttt
     1681 tgcaaagata gtatcttggt tgcccacaac gccagttttg acgtgggctt tatgaacgcc
     1741 aattatgaac gccacgactt gcccaaaatc acacagcctg tgattgatac cttagaattt
     1801 gctagaaact tgtatcctga atacaagcgt cacggtttgg gaccgctcac caagcgtttc
     1861 caagtgagtc tagaccacca tcatatggcc aattacgacg cggaagccac aggacgtctt
     1921 ttgtttattt ttctaagaga tgccagagaa aagcatggca tcaaaaatct tttgcaactc
     1981 aatacagatt tggtggctga ggattcttac aaaaaagcgc ggattaagca tgcgactatc
     2041 tatgtgcaaa atcaggttgg tcttaaaaat atgtttaagt tggtcagcct ttccaatatc
     2101 aaatattttg aaggggtgcc gcgtattcca agaaccgtct tagatgctca cagagagggt
     2161 ttgttactag gtacagcttg ttctgacggc gaggtttttg atgccgttct gactaaagga
     2221 attgatgcgg cggttgattt agctaggtat tatgatttta tcgaaatcat gccaccagcc
     2281 atttaccagc cattggttgt ccgtgaatta atcaaagatc aagcaggtat tgagcaggtg
     2341 attcgtgacc tcattgaagt agggaaacga gctaagaaac ctgtgcttgc cactgggaat
     2401 gtgcattatc tagagcctga agaagagatt taccgtgaaa ttattgtgcg tagtctcggt
     2461 cagggtgcca tgattaatag aacaatcggc cgtgggggag gggcacagcc tgctcctcta
     2521 cctaaggcgc actttagaac aaccaatgaa atgctggatg agtttgcctt tcttggaaaa
     2581 gacctcgctt atcaagtggt tgtgcaaaat actcaggatt ttgcggaccg tattgaggaa
     2641 gtggaagtgg ttaagggcga tctttacact ccgtatattg acaaggccga agagacggtt
     2701 gccgaattaa cctatcaaaa agcctttgaa atttatggta atcctctacc agatattatc
     2761 gatttgcgaa ttgaaaaaga gctaacctct atcttgggga acggctttgc cgtgatttac
     2821 ctagcgtcac aaatgcttgt taaccggtca aatgagcgag gctatctggt tggttctagg
     2881 ggatctgttg ggtccagttt tgtggcaacc atgatcggga ttactgaggt taatcctatg
     2941 ccgcctcact acgtttgccc gtcctgccaa cattctgaat ttatcacaga tggttcagtc
     3001 ggatcgggct atgatttacc taataagcct tgtccaaaat gtggcacccc ttatcaaaaa
     3061 gacggccaag atattccctt tgagaccttc cttggatttg acggggataa ggtgcccgat
     3121 attgatttga acttctctgg tgatgaccaa cccagtgccc atttggatgt ccgagatatt
     3181 tttggtgacg aatacgcctt tcgtgctgga accgtaggta ccgtggcaga aaaaacagct
     3241 tatggatttg ttaaaggcta tgaacgcgac tatggcaagt tctatcggga tgctgaggtg
     3301 gatcgtctag cagcaggtgc tgctggtgtg aaacgaacga ctgggcagca ccctgggggg
     3361 attgttgtta ttcctaatta catggatgtt tatgatttta cccccgtgca atatccagcc
     3421 gatgatgtaa cggcttcttg gcagacaact cactttaact tccatgatat tgatgaaaac
     3481 gtcttgaaac ttgatatcct agggcatgat gatccgacca tgattcgtaa acttcaggat
     3541 ttatcgggca ttgatcctat tactattcct gctgatgatc cgggggttat ggctctcttt
     3601 tctgggacag agattttggg cgttaccccg gaacaaattg ggacaccgac tggtatgcta
     3661 ggcattccag aatttggaac caactttgtt cgcggcatgg ttaatgagac gcatccgacc
     3721 acttttgcag aattgttgca gttgtctgga ctatctcatg gaaccgatgt ttggcttggc
     3781 aatgcacaag atttgattaa agaaggcatt gcgaccctaa aaaccgttat cggttgtcgt
     3841 gacgacatca tggtttacct catgcacgct ggcttgcaac cgaaaatggc ctttaccatt
     3901 atggagcgtg tgcgtaaggg attatggcta aaaatttctg aggaagaacg taatggctat
     3961 attgatgcca tgcgagaaaa caatgtgccc gactggtaca ttgaatcgtg tggaaaaatc
     4021 aagtacatgt tccctaaagc ccatgcggca gcttatgtct tgatggccct tcgggtggct
     4081 tatttcaagg tgcactatcc cattatgtat tattgtgctt atttctctat tcgtgcgaag
     4141 gcttttgaat taaaaaccat gagtggtggc ttagatgctg ttaaagcaag aatggaagat
     4201 attactataa aacgtaaaaa taatgaagcc accaatgtgg aaaatgacct ctttacaacc
     4261 ttggagattg tcaacgaaat gttagaacgc ggctttaagt ttggcaaatt agacctttac
     4321 aaaagtgatg ctatagaatt ccaaatcaaa ggagataccc ttatccctcc atttatagcg
     4381 ttagaaggtc tgggtgaaaa cgtggccaag caaatcgtta aagctcgtca agaaggcgaa
     4441 ttcctctcta aaatggaatt gcgtaaacga ggcggggcat cgtcaacgct cgttgagaaa
     4501 atggatgaga tgggtatttt aggaaatatg ccagaagata atcaattaag tctttttgat
     4561 gactttttct agattatcat gatggtagtc atatgcaaag tcataaaaga agagggacaa
     4621 gatcactttc taatctctct gatactaaac tgtgacatac cgtataaagc gaggaaatca
     4681 aatgagtcat ttagataaga acaccgcttt gaaagccatg gtggtgtttc gtaaagctca
     4741 gcgaacgcta gatgcttttg gggctgatat ttttaaaaag gcagatttaa cagccacgca
     4801 gttcagtgtg cttgaagtac tatatactaa aggctgtatg cgaatcaatc acttgattga
     4861 ttctttgtta gcgacttcgg ggaatatgac tgttgtacta aataatatgg aacgtaatgg
     4921 ctggattagc aagtgcaaag acaaaacgga taaaagagcc tatgtggtta ccttaacaga
     4981 taagggtact cgcttgattg aagctgtttt gcctaaacac gtcgcacggg tggaagaagc
     5041 ctttgctgtt ttgactgaaa aagaacaact ttgtctgatt gaacttctca aaaagttcaa
     5101 acaattataa acaaatctct ttacggctct tcttagagaa gatataatta ctacatagtg
     5161 ataaatttca aaaagaagta tcaatgaaat accactttta gaaagcttta aaacgatctt
     5221 taaaaaagag gacaaagaga tgaaacatat tttatttatt gttggctcgc ttcgtgaagg
     5281 gtcttttaac catcaattag cggctcaagc acaaaaagct ctggaacatc aagcagttgt
     5341 atcttactta aattggaaag acgttcctgt tttgaatcaa gatatcgaag ctaatgcacc
     5401 tttaccagtt gttgacgctc gtcaagctgt tcagtcagcg gatgctatct ggatttttac
     5461 accagtttac aacttctcta ttccaggttc tgttaaaaac ctgctagact ggttgtctcg
     5521 tgctcttgat ttgtctgatc cgacgggccc atctgctatt ggcggtaagg tggttacggt
     5581 ctcttcagtt gcaaatggcg ggcatgatca agtatttgat cagtttaaag cactattgcc
     5641 gtttatccga acttcagtag caggagagtt tacaaaagca actgtgaatc ctgatgcctg
     5701 gggaacagga aggcttgaga tttcaaaaga gacaaaagca aacttgctat ctcaggcaga
     5761 ggctctt
//
LOCUS       DAUCLR010000098         5648 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.099, whole genome shotgun sequence.
ACCESSION   DAUCLR010000098 DAUCLR010000000
VERSION     DAUCLR010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5648
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.099"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..769)
                     /locus_tag="VGT87_001617"
     CDS             complement(<1..769)
                     /locus_tag="VGT87_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEQ3814451.1"
                     /translation="MIILQGNKLERSFSGDVLFQNISLQVDERDRIALVGPNGAGKST
                     LLKLLVGEETPTSGEVNTKKDLTLSYLAQNSRFESDQTIYEEMLKVFEALRQDEKRLR
                     QMEMDMATVSGQDLTRLMTDYDLLTEHFRQQGGFTYESDIKAILNGFKFDESMWQMTI
                     AELSGGQNTRLALAKMLLEKPELLVLDEPTNHLDIETIAWLENYLANYQGALIIVSHD
                     RYFLDKVATVTLDLTPHGLDRYVGNYSRFLTLKAEKLV"
     gene            complement(815..1141)
                     /locus_tag="VGT87_001618"
     CDS             complement(815..1141)
                     /locus_tag="VGT87_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814452.1"
                     /translation="MKQLLAIILWLPKLIVKMFWHLIKGFLQTILLVTIIIIGLMYYS
                     NHSDSVLANKISIVTEQVVQIFDILTQKPSAKTRHGSGNSHSTFLVSAKEGNQRLKKS
                     SISFGN"
     gene            complement(1171..1956)
                     /locus_tag="VGT87_001619"
     CDS             complement(1171..1956)
                     /locus_tag="VGT87_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEQ3814453.1"
                     /translation="MANIAIEYHSVVLGMERKVNVIYPDQSEIPKKDQGDKDIPVLYL
                     LHGMGGNENSWQKRAAIERLLRHTNLIVVMPSTDLGWYTDTAYGLNYYRALSQELPQV
                     LAAFFPNMTQKREKTFVAGLSMGGYGAFKWALKSNRFSYAASFSGALDFSPETLLEGN
                     LGELAYWQGVFGQFDDPDLDKHYLKNMVAESDGKTKFYAWCGYEDFLFATNEKAIADF
                     QAQGLDIDYHKGHGKHEWYYWNQQLEVLLEWLPINYQKEERLS"
     gene            complement(2089..3750)
                     /locus_tag="VGT87_001620"
     CDS             complement(2089..3750)
                     /locus_tag="VGT87_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEQ3814454.1"
                     /translation="MTDIKMIALGGVREYGKNFYLVEINDSMFILDAGLKYPENEQLG
                     VDLVIPNLDYVIENKGKVQGIFLSHGHADAIGALPYLLAEVSAPVFGSELTIELAKLF
                     VKSNNSTKKFNNFHVVDSDTEIEFKDGLVSFFRTTHSIPESMGIVIGTDKGNIVYTGD
                     FKFDQAAREGYQTDLLRLAEIGKEGVLALLSDSVNATSNDQIASESEVGEEMDSVISD
                     ADGRVIVAAVASNLVRIQQVFDSATAHGRRVVLTGTDAENIVRTALRLEKLMITDERL
                     LIKPKDMSKFEDHELIILEAGRMGEPINSLQKMAAGRHRYVQIKEGDLVYIVTTPSTA
                     KEAMVARVENLIYKAGGSVKLITQNLRVSGHANGRDLQLLMNLLKPQYLFPVQGEYRD
                     LAAHAKLAEEVGIFPENIHILKRGDIMVLNDEGFLHEGGVPASDVMIDGNAIGDVGNI
                     VLRDRKVLSEDGIFIVAITVSKKEKRIISKAKVNTRGFVYVKKSHDILRESAELVNTT
                     VGNYLKKDTFDWGELKGNVRDDLSKFLFEQTKRRPAILPVVMEVR"
     gene            complement(3954..4712)
                     /locus_tag="VGT87_001621"
     CDS             complement(3954..4712)
                     /locus_tag="VGT87_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ3814455.1"
                     /translation="MTKIIELINATVDVDNGFEDAKTILDNVTLTIYEHDFLTILGGN
                     GAGKSTLFNVIAGTLSLTRGQIRILGQDVTHWPAEKRALYLSRVFQDPKMGTAPRMTV
                     AENLLIARQRGEKRSLASRKITEHLASFEDLVKRTGNGLEKHLETPAGLLSGGQRQAL
                     SLLMATLKKPALLLLDEHTAALDPKTSQSLMQLTDEFVTKDGLTALMITHHMEDALTY
                     GNRLIVMKDGNIIKDLNQMEKEQLTITDYYQLFD"
     gene            complement(4709..5578)
                     /locus_tag="VGT87_001622"
     CDS             complement(4709..5578)
                     /locus_tag="VGT87_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ3814456.1"
                     /translation="MIISSVSQGLIWGVLGLGIYLTFRILNFPDMTTEGSFPLGGAVA
                     VTAISLGWNPFLSTLLGMLSGALAGFLTGLLYTKGKMPTLLAGILVMTSCNSIMLMVM
                     GRANLGLHDHKRIQDCFPFSIDLNSLLTGLITVVIVISVLIYFLYTNLGQAYIATGDN
                     KDMAKSFGINTDWMEVMGLVVSNSLIALSGALVSQQDGYADVSKGIGVIVIGLASIIV
                     GEVLYSTGLTLLERLIAIVIGSILYQFLISVVITLGFNTSYLKLISALVLALCLMIPV
                     VKERFFKGVRLTR"
BASE COUNT         1728 a         1242 c          911 g         1767 t
ORIGIN      
        1 ctactaattt ttcagctttt aaggtcagaa aacgagaata attgccaacg tagcgatcca
       61 agccatgagg agttaaatcc aaggtcactg ttgccacctt atccaaaaaa taacggtcgt
      121 gactcacaat gattagagct ccttgataat tggccaagta gttctctaac caggcaatgg
      181 tttcaatatc taagtggttt gttggctcat caaggaccaa tagttctggc ttttctaaaa
      241 gcattttagc caaggccaga cgagtgtttt gaccccctga cagctcagcg atagtcatct
      301 gccacatgct ctcatcaaac ttaaacccat ttaaaatggc tttgatgtca gattcatacg
      361 taaagccgcc ttgctgacga aaatgctctg taagcagatc ataatctgtc atcagtcggg
      421 ttaaatcttg acctgaaacg gttgccatat ccatttccat ctgacgcagg cgcttttcat
      481 cttggcgtaa tgcttcgaaa accttgagca tttcctcata tattgtttga tctgattcaa
      541 aacggctatt ttgagccaga taagacaagg ttaggtcttt tttagtatta acttctccgc
      601 tcgttggggt ttcttcccca accaatagct tgagtaaggt tgacttacca gctccatttg
      661 gccccactaa tgcaattctg tctctttcat caacttgcag tgatatgttt tgaaaaagaa
      721 catctcctga gaaagagcgt tctaacttgt ttccttgtaa aataatcata ctttcattct
      781 aacaaaaaaa tgcccttttg aggacatttt cagcctagtt tccaaaagaa atcgaactct
      841 ttttaagtcg ttgattacct tctttagcgc taacaaggaa agtggaatga ctattaccac
      901 tcccatgacg cgtctttgca gatggctttt gtgttaagat atcaaaaatt tgcaccactt
      961 gctcagtaac tatcgagatc ttattggcta aaacggaatc actgtgatta gaatagtaca
     1021 tcagccctat aataataatc gttactaata agatagtttg taaaaaccct ttgataagat
     1081 gccaaaacat tttcactatt agcttaggca gccacaatat gatcgccagt aattgtttca
     1141 tttgtcacct catgttgtac taggaaatgg ttaagacaac ctttcttctt tttggtagtt
     1201 tatcggtaac cactctaaca ggacttctaa ttgctggttc caatagtacc actcatgttt
     1261 accatgacct ttatgataat caatgtcaag accttgagcc tgaaaatcag caatggcttt
     1321 ttcattagtg gcaaaaagaa aatcctcata gccacaccaa gcataaaatt tggtttttcc
     1381 atcactttct gccaccatat ttttaaggta atgcttatca agatcaggat catcaaactg
     1441 gccaaaaacg ccctgccaat aggctaattc tcccaggttt ccctcaagca aggtctcagg
     1501 agaaaaatcc aaagcgcctg aaaacgatgc cgcataagaa aaccgattgc ttttgagtgc
     1561 ccacttaaag gcaccatagc cacccataga gagcccagca acaaaggttt tttctcgttt
     1621 ctgggtcata ttaggaaaga aagctgctaa gacctgcggt aactcttgtg acaacgcccg
     1681 ataatagtta agtccataag ccgtgtctgt ataccaacct aggtctgtcg aaggcataac
     1741 gactatcaaa ttggtatgcc gcaacaagcg ttcaatagct gctcgttttt gccaagagtt
     1801 ttcattgcct cccataccat gtaacaaata taaaacagga atatccttat ccccttgatc
     1861 ttttttaggg atttcagact ggtcaggata aatcacattt accttacgct ccattcctaa
     1921 aactaccgaa tggtattcaa ttgcaatatt tgccattcat tacactcctt tataccaaaa
     1981 taatcctatc acgtaaatct actgtatttt tgggtgaaca gtttcctggt ttaccagttg
     2041 ttaaaatcaa agaaggtagg cagtttttgc ctaaccttcc tattttcact aacgaacttc
     2101 catcacaacc ggcagaatcg ctgggcgcct tttggtttgt tcaaataaga atttagacaa
     2161 atcatctctg acattccctt ttaattcgcc ccagtcaaag gtatcttttt ttaagtaatt
     2221 gccaacagtt gtattaacta attctgcact ttctcttaaa atatcatgac ttttcttgac
     2281 atacacaaat ccacgcgtat taactttagc cttagaaata atgcgttttt cttttttaga
     2341 aacagtaata gcaacaatga aaataccatc ttctgacaag actttacggt ctcgtaagac
     2401 gatattaccc acatcaccga tagcattacc gtcaatcatg acatcactgg caggtacacc
     2461 tccttcatgg agaaaacctt cgtcattaag caccatgata tcgccacgct tcagaatatg
     2521 aatattctct ggaaaaatac caacttcttc agctagcttt gcatgggcag ctaaatcacg
     2581 atattctcct tgtactggaa aaagatactg gggttttaac aaattcatca atagttgcaa
     2641 atcacgccca ttagcatggc ctgacacgcg taagttttga gtgattaatt taacagaacc
     2701 accggccttg taaatcaagt tttcaacacg cgccaccata gcttctttag ccgtacttgg
     2761 tgttgttaca atataaacaa ggtctccttc tttgatttga acataacggt gacgtcccgc
     2821 tgccattttt tgtaagctat taattggctc acccatacga ccagcttcaa ggatgattaa
     2881 ttcatggtct tcgaatttag acatatcctt aggtttaatt aaaagacgct catccgtaat
     2941 cattaatttt tctagacgta aagctgttct gacaatgttt tctgcatctg ttccggttaa
     3001 aacaacacga cgcccatggg ctgtggcgga atcaaatact tgctgaatcc gaacaagatt
     3061 tgaggctact gcagctacaa tgacacggcc atcagcatca gagataacac tgtccatttc
     3121 ttcaccaact tcagattcgc tggcaatctg atcgttgctt gtcgcattga ctgaatctga
     3181 taaaagagct agaacccctt ctttcccaat ctcagctaaa cgtaacaaat ccgtctgata
     3241 accttctctt gctgcttgat caaatttgaa gtcaccggta taaacaatat ttcctttatc
     3301 tgttccaata acaataccca tactttctgg gattgaatgc gttgttctaa agaacgaaac
     3361 tagcccatct ttaaattcaa tttcagtatc actgtctacc acatgaaaat tattaaattt
     3421 cttagtgctg ttattacttt tcacaaatag cttagctaat tcaatagtca attcagatcc
     3481 aaatacagga gctgatactt cagctaacaa ataaggaagg gctccaatgg cgtcagcatg
     3541 gccatgactc aagaaaatcc cttgtacttt acctttgttt tcaattacat aatctaaatt
     3601 ggggatgact aaatctaccc caagttgctc attttcgggg tacttgagcc ctgcgtctaa
     3661 aataaacata gaatcgttaa tttctaccaa atagaagttt tttccgtatt cacggacccc
     3721 tcccaaggca atcattttaa tatcagtcat gatactcctt taaatatttt attattacaa
     3781 acggtcctta caacgtaagt cgaattactg ttatgtagct atgctactat cttctatagt
     3841 atatcatatt tgaggcgttt tttctctaaa tagccttgat actttcagat tattttcagt
     3901 acagaaatca aaaaatcact gacttattat tagtcaatga tttagatcgt tgactaatca
     3961 aatagttggt aatagtcagt aatcgtcagt tgttcttttt ccatttgatt taaatcttta
     4021 ataatgtttc catctttcat tacaatcaga cgattcccat aagttaatgc atcttccata
     4081 tggtgggtaa tcatcaaagc tgttaagcca tcttttgtca caaactcatc tgtcagttgc
     4141 attaaagatt gactcgtctt agggtcaagg gcagccgtat gctcatctaa taataagaga
     4201 gctggctttt tcaacgttgc cattaataaa cttaaagcct gtctttgtcc accagacaaa
     4261 agacctgctg gtgtttctaa atgcttttct aggccatttc ctgttcgttt aaccaaatcc
     4321 tcaaaactag ctaagtgctc tgttattttt cgagaagcca aggagcgttt ttctcctctt
     4381 tgacgtgcga ttaagagatt ttcagccact gtcatccgag gggctgttcc cattttggga
     4441 tcttgaaaga ctcgcgatag gtaaagagct cttttttctg ccggccaatg cgtcacatcc
     4501 tgaccaagaa tcctaatttg cccccgtgtc aaacttagcg ttcctgcaat tacattaaaa
     4561 agggttgatt ttccagctcc atttcctcct aaaatagtta aaaaatcatg ttcatagata
     4621 gttagtgtca cattgtccaa tattgtttta gcatcttcaa aaccattatc tacgtcaact
     4681 gttgcattga tcaattcaat aatttttgtc atcgtgttaa cctcactccc ttaaagaagc
     4741 gctcttttac cactggtatc atgaggcaca gtgctaaaac aagagcacta attaatttca
     4801 aataacttgt attaaaacct agcgtaataa ctactgaaat taaaaattga taaagaatcg
     4861 agcctattac aatagcaatc aaacgttcta aaagagtcag tccagttgag tatagtactt
     4921 caccaacaat tatgctcgcc aaaccaataa caataactcc aatccctttt gagacatctg
     4981 catagccatc ttgttgactc actagagcac ctgataaagc tattaaacta ttagaaacca
     5041 caagtcccat aacttccatc caatccgtgt taataccaaa actcttagcc atatctttat
     5101 tgtctcccgt agcaatataa gcttgaccta gattagtgta aaggaaatag atcaaaacgc
     5161 taataacaat cacaacagtt atcagacctg ttaaaaggct atttaaatca attgaaaaag
     5221 ggaaacaatc ctgaatacgt ttatgatcat gtaaacccaa gtttgctctt cccattacca
     5281 ttagcataat ggagttacat gacgtcatca ccaaaattcc agctaaaagt gttggcattt
     5341 ttcctttcgt atataacagc cctgttagaa aacctgctaa agcacctgat aacataccaa
     5401 gtaaggttga tagaaatgga ttccacccta agctaatagc ggtgaccgca acagctcctc
     5461 ctaaaggaaa agaaccttct gtagtcatgt cagggaagtt taaaatccta aaggtcaggt
     5521 agattcctaa tcctaaaact ccccatataa ggccttgtga aactgatgaa ataatcatat
     5581 ttttcctcat atttatatta gcgggtaacg ctcgccagta taaacccttt ctttacatta
     5641 atttttag
//
LOCUS       DAUCLR010000099         5496 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.100, whole genome shotgun sequence.
ACCESSION   DAUCLR010000099 DAUCLR010000000
VERSION     DAUCLR010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5496
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.100"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            830..3856
                     /locus_tag="VGT87_001623"
     CDS             830..3856
                     /locus_tag="VGT87_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HEQ3814457.1"
                     /translation="MKKHLKTVALTLTTVSVVTHNQEVFSLVKEPILKQTQASSSISG
                     ADYAESSGKSKLKINETSGPVDDTVTDLFSDKRTTPEKIKDNLAKGPREQELKAVTEN
                     TESEKQINSGSQLEQSKESLSLNKRVPSTSNWEICDFITKGNTLVGLSKSGVEKLSQT
                     DHLVLPSQAADGTQLIQVASFAFTPDKKTAIAEYTSRAGENGEISQLDVDGKEIINEG
                     EVFNSYLLKKVTIPTGYKHIGQDAFVDNKNIAEVNLPESLETISDYAFAHLALKQIDL
                     PDNLKAIGELAFFDNQITGKLSLPRQLMRLAERAFKSNHIKTIEFRGNSLKVIGEASF
                     QDNDLSQLMLPDGLEKIESEAFTGNPGDDHYNNRVVLWTKSGKNPYGLATENTYVNPD
                     KSLWQESPEIDYTKWLEEDFTYQKNSVTGFSNKGLQKVKRNKNLEIPKQHNGVTITEI
                     GDNAFRNVDFQNKTLRKYDLEEVKLPSTIRKIGAFAFQSNNLKSFEASDDLEEIKEGA
                     FMNNRIETLELKDKLVTIGDAAFHINHIYAIVLPESVQEIGRSAFRQNGANNLIFMGS
                     KVKTLGEMAFLSNRLEHLDLSEQKQLTEIPVQAFSDNALKEVLLPASLKTIREEAFKK
                     NHLKQLEVASALSHIAFNALDDNDGDEQFDNKVVVKTHHNSYALADGEHFIVDPDKLS
                     STMVDLEKILKLIEGLDYSTLRQTTQTQFRDMTTAGKALLSKSNLRQGEKQKFLQEAQ
                     FFLGRVDLDEAIAKAEKALVTKKATKNGQLLERSINKAVLAYNNSAIKKANVKRLEKE
                     LDLLTGLVEGKGSLAQATMVQGVYLLKTPLPLPEYYIGLNVYFDKSGKLIYALDMSDT
                     IGEGQKDAYGNPILNVDEDNEGYHALAVATLADYEGLDIKTILNSKLIQLTSIRQVPT
                     AVYHRAGIFQAIQNAAAEAEQLLPKPGTHSEKSSSSESANSKDRGLQSHPKTNRGRHS
                     AILPRTGSKGSFVYGILGYTSVALLSLIAAIKKKKY"
     gene            3876..4277
                     /locus_tag="VGT87_001624"
     CDS             3876..4277
                     /locus_tag="VGT87_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEQ3814458.1"
                     /translation="MITQEMKDLINNQLAMVATVDAKGQPNIGPKRSMRLWDDKTFIY
                     NENTDGQTRINIEDNGKIEIAFVDRERLLGYRFVGTAEIQTEGAYYEAAKKWAQGRMG
                     VPKAVGIIHVERIFNLQSGANAGKEINSESN"
     gene            complement(4457..5332)
                     /locus_tag="VGT87_001625"
     CDS             complement(4457..5332)
                     /locus_tag="VGT87_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEQ3814459.1"
                     /translation="MPASKKVTIIFILNLSFSLIEFIFGTLFFSGAILADAVHDFGDA
                     IAIGISAILERKAVKKESPNFSLGYKRFSLLGALTTNLILISGSLLVMIETIPKLWHP
                     TIVNYDGMFVLAIFAIIINGFASFIIHSNQTKNEEILSLHFLEDILGWLAIIILSLIL
                     KWKPWYILDPLLSIAIASFILSKALPKLVATANIFLDGVPDSIDYCALHHELSQLPHI
                     VSVNQLNVWSMDGIDHRATIHCCLRESTTEKHCKKSIRLICQRYNINSVTVEIDTSLN
                     EHQHHCSSLSSIKVN"
BASE COUNT         1942 a          846 c         1029 g         1679 t
ORIGIN      
        1 ctgtctttaa ctaaaaagaa gttaaagaat ggcttagctg attgccttta taagagatta
       61 tactggtttt tgatagctgc tgtcatgtaa aatagctaca attcatctta cgagattgac
      121 cttagcattg ccagtgcagt ctttattttc caacctgctg tttgttgtat gtttatagtt
      181 tcctaaaaaa tgctctctca gttttttggg ggacattcct aaaattaagt atacttaatt
      241 ttaggaatgt ttatattctt tacattctga tgaaaataca aattatccta acatgatgta
      301 ttgaaaggag aaatagttta tgttagtata ctgattaagt gaagctgtaa tgaggcttat
      361 acgatcaaaa aagttagagt aactttataa aacagtggtg ttgaagcaag tttagcttct
      421 tctcagaaat agattatcta taaaaaatcc caaaagtagt ttgtttccag ttaatgaact
      481 tcttttggga aggctttttt gctgatcgcc acttcacttg tatatcggga acattcacgc
      541 taagatgtat aagttgaatc gtttgtctat ggcttgatga aaattaatca aaagataatg
      601 ttcaactcta taattagtac aagcaaaatg agataactta ttcattagta agcataagat
      661 ataacaagag aaattacctt aaaacaggaa gtactagagt atttaaaata agtgatagac
      721 ttggatatta ttaggtcgac tagtgtcctt ttcaataatt taatatcgtt attattagta
      781 tcagccaatc aaatgtggat aagttggctt ttagaaaggt tttaccacaa tgaagaaaca
      841 tcttaaaaca gttgccttga ccctcactac agtatcggta gtcacccaca atcaggaagt
      901 ttttagttta gtcaaagagc cgattcttaa acaaactcaa gcttcttcat cgatttctgg
      961 cgctgactac gcagaaagta gcggtaaaag caagttaaag attaatgaaa cttctggccc
     1021 tgttgatgat acagtcactg acttattttc ggataaacgt actactcctg aaaaaataaa
     1081 agataatctt gctaaaggtc cgagagaaca agagttaaag gcagtaacag agaatacaga
     1141 atcagaaaag cagatcaatt ctggatctca actagaacaa tcaaaagagt ctctttcttt
     1201 aaataaaaga gtgccatcaa cgtctaattg ggagatttgt gattttatta ctaaggggaa
     1261 tacccttgtt ggtctttcaa aatcaggtgt tgaaaagtta tctcaaactg atcatctcgt
     1321 attgcctagt caagcagcag atggaactca attgatacaa gtagctagct ttgcttttac
     1381 tccagataaa aagacggcaa ttgcagaata taccagtagg gctggagaaa atggggaaat
     1441 aagccaacta gatgtggatg gaaaagaaat tattaacgaa ggtgaggttt ttaattctta
     1501 tctactaaag aaggtaacaa tcccaactgg ttataaacat attggtcaag atgcttttgt
     1561 ggacaataag aatattgctg aggttaatct tcctgaaagc ctcgagacta tttctgacta
     1621 tgcttttgct cacctagctt tgaaacagat cgatttgcca gataatttaa aagcgattgg
     1681 agaattagct ttttttgata atcaaattac aggtaaactt tctttgccac gtcagttaat
     1741 gcgattagca gaacgtgctt ttaaatcaaa ccatatcaaa acaattgagt ttagaggaaa
     1801 tagtctaaaa gtgatagggg aagctagttt tcaagataat gatctgagtc aactaatgct
     1861 acctgacggt cttgaaaaaa tagaatcaga agcttttaca ggaaatccag gagatgatca
     1921 ctacaataac cgtgttgttt tgtggacaaa atctggaaaa aatccttatg gtcttgctac
     1981 tgaaaatacc tatgttaatc ctgataagtc actatggcag gaaagtcctg agattgatta
     2041 tactaaatgg ttagaggaag attttaccta tcaaaaaaat agtgttacag gtttttcaaa
     2101 taaaggctta caaaaagtaa aacgtaataa aaacttagaa attccaaaac agcacaatgg
     2161 tgttactatt actgaaattg gtgataatgc ttttcgcaat gttgattttc aaaataaaac
     2221 tttacgtaaa tatgatttgg aagaagtaaa gcttccctca actattcgga aaataggtgc
     2281 ttttgctttt caatctaata acttgaaatc ttttgaagca agtgacgatt tagaagagat
     2341 taaagaggga gcctttatga ataatcgtat tgaaaccttg gaattaaaag ataaattagt
     2401 tactattggt gatgcggctt tccatattaa tcatatttat gccattgttc ttccagaatc
     2461 tgtacaagaa atagggcgtt cagcatttcg gcaaaatggt gcaaataatc ttatttttat
     2521 gggaagtaag gttaagacct taggtgagat ggcattttta tcaaatagac ttgaacatct
     2581 ggatctttct gagcaaaaac agttaacaga gattcctgtt caagcctttt cagacaatgc
     2641 cttgaaagaa gtattattac cagcatcact gaaaacgatt cgagaagaag ccttcaaaaa
     2701 gaatcattta aaacaactgg aagtggcatc tgccttgtcc catattgctt ttaatgcttt
     2761 agatgataat gatggtgatg aacaatttga caataaagtg gttgttaaaa cgcatcataa
     2821 ttcctacgca ctagcagatg gtgagcattt tatcgttgat ccagataagt tatcttctac
     2881 aatggtagac cttgaaaaga ttttaaaact aatcgaaggt ttagattatt ctacattacg
     2941 tcagactact caaactcagt ttagagacat gactactgca ggtaaagcgt tgttgtcaaa
     3001 atctaacctc cgacaaggag aaaaacaaaa attccttcaa gaagcacaat ttttccttgg
     3061 ccgcgttgat ttggatgaag ccatagctaa agctgagaag gctttagtga ccaagaaggc
     3121 aacaaagaat ggtcagttgc ttgaaaggag tattaacaaa gcggtattag cttataataa
     3181 tagcgctatt aaaaaagcta atgttaagcg cttggaaaaa gagttagact tgttaacagg
     3241 attagttgag ggaaaaggat cattagcgca agctacaatg gtacaaggag tttatttatt
     3301 aaagacgcct ttgccattgc cagaatatta tatcggattg aacgtttatt ttgacaagtc
     3361 tggaaaattg atttatgcac ttgatatgag tgatactatt ggcgagggac aaaaagacgc
     3421 ttatggtaat cctatattaa atgttgacga ggataatgaa ggttatcatg ccttggcagt
     3481 tgccacttta gctgattatg aggggctcga catcaaaaca attttaaata gtaagcttat
     3541 tcaattaaca tctattcgtc aggtaccgac tgcagtctat catagagccg gtattttcca
     3601 agctatccaa aatgcagcgg cagaagcaga acagttattg cctaaaccag gtacgcactc
     3661 tgagaagtca agctcaagtg aatctgctaa ctctaaagat agaggattgc aatcacaccc
     3721 aaaaacgaat agaggacgac actctgcaat attgcctagg acagggtcaa aaggcagctt
     3781 tgtctatgga atcttaggtt acactagcgt tgctttactg tcactaatag ctgctataaa
     3841 aaagaaaaaa tattaaattg atttaaggag aaaaaatgat aacacaagaa atgaaagatc
     3901 ttattaataa ccagttagca atggttgcga cggttgatgc caaaggtcaa cccaatattg
     3961 gtccaaaacg ttctatgcgt ctctgggatg ataaaacatt tatttataat gaaaatacag
     4021 atggtcaaac tcgtattaac attgaagata atgggaaaat tgaaattgcc tttgttgatc
     4081 gtgagcgcct tttaggttac aggtttgtag gaactgctga aattcaaaca gaaggtgctt
     4141 attacgaagc agcgaaaaaa tgggcacaag gacgcatggg ggtaccaaaa gcagttggta
     4201 ttattcatgt cgagcgtatt tttaatttac aatctggtgc taatgcaggt aaagaaataa
     4261 acagtgaaag taactaaaat taactgtaac agtatttagg acccttattt taaacgctaa
     4321 aaaacaagca tactcttttt tacagcatag tgactatgtt gtaaaaaggg gtatgcttat
     4381 taggtgctct taatatttct agataactgc atctaataag tttttaatag acttatagca
     4441 cattaataga cttcaattag ttgactttaa tactagaaag agaagaacag tgatgttggt
     4501 gttcgtttaa agaagtatcg atttccacag tgactgagtt tatattgtac ctttgacaaa
     4561 tcagtctaat agattttttg caatgttttt cagtggtaga ttctctcaag cagcaatgta
     4621 tagttgctct atgatcaata ccatccatcg accaaacatt aagctgatta acagacacta
     4681 tatgaggaag ttggctgagt tcatggtgca aagcgcaata gtctatcgaa tcaggaacac
     4741 catctaaaaa aatgttagca gttgctacta actttggaag agctttagat aatataaaag
     4801 aagcaatggc aattgataat aaaggatcaa gaatgtacca aggtttccat tttaagatca
     4861 gtgacaatat gataatggct aaccacccaa ggatatcttc taaaaagtga aggcttaata
     4921 tttcttcatt ttttgtctgg ttagagtgaa tgatgaagct agcaaatcca ttgattataa
     4981 ttgcaaaaat agctaaaacg aacataccgt cataattaac aatagtagga tgccataatt
     5041 ttggtatcgt ttcaatcatt actaataatg acccgctaat aagtattaga tttgttgtta
     5101 gtgctcctaa aaggctaaat cgcttatagc ctagtgaaaa atttggactc tcttttttaa
     5161 cagcctttct ttctaagata gctgagatac caatagcaat ggcatctcca aaatcgtgga
     5221 cagcatctgc taaaatagca cccgaaaaga ataatgttcc aaaaataaat tcaatgaggg
     5281 aaaaactaag atttaatata aaaataatgg tgactttttt acttgctggc ataattttct
     5341 ccttttgtta tactggtaat atgtatagta gaattcttta ttctattatc tagaagttaa
     5401 aatacaatta ctataaccaa aaatgagcac ctgttcaata gtgaacaaca tttagaaagt
     5461 gtccgatatg acaaaagaca gacaaatcaa aaaaac
//
LOCUS       DAUCLR010000100         5317 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.101, whole genome shotgun sequence.
ACCESSION   DAUCLR010000100 DAUCLR010000000
VERSION     DAUCLR010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5317
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.101"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(140..526)
                     /locus_tag="VGT87_001626"
     CDS             complement(140..526)
                     /locus_tag="VGT87_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEQ3814460.1"
                     /translation="MKLQLTNHIRFGDETEIIQEIHDCEWREKGGYQYLIYQNTDKEK
                     VVIKYNETELTMSRFSNPQSIMKFFAGKKVLIALPTPMGVQQFLTDTSHYHLDCSCQK
                     LDLHYHLLQAQTEMLFASYHLELSWD"
     gene            608..1909
                     /locus_tag="VGT87_001627"
     CDS             608..1909
                     /locus_tag="VGT87_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEQ3814461.1"
                     /translation="MNEKVFRDPVHNYIHIDNPLIYDLINTKEFQRLRRIKQVPTTAF
                     TFHGAEHSRFSHCLGVYEIARRVTAIFEEKYADIWNKDESLVTMTAALLHDIGHGAYS
                     HTFEALFHTDHEAFTQEIITNPETEINAILVRHALDFPDKVASVINHTYPNKQVVQLI
                     SSQIDCDRMDYLLRDSYFSAANYGQFDLMRILRVIRPVEDGIVFEHSGMHAVEDYIVS
                     RFQMYMQVYFHPASRAVELILQNLLKRAQHLYPEQQAYFQKTAPGLIPLFEKKANLAD
                     YIALDDGVMNTYFQVWMASEDHILSDLASRFINRKILKSVTFDQDSQGELERLRQLVE
                     SVGFDPDYYTGIHINFDLPYDIYRPELENPRTQIEMIQKDGSLAELSQLSPIVKALTG
                     TTYGDRRFYFPKEMLELDDLFAPSKETFMSYISNGHFHFSQ"
     gene            1981..2214
                     /locus_tag="VGT87_001628"
     CDS             1981..2214
                     /locus_tag="VGT87_001628"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814462.1"
                     /translation="MEFYALFLSLGSQKFRVTSLPVLTPLLPQQKAAVLAHVTRRNKK
                     SVVKSAEQFMLFEHQTGFVSILRLKKEYFLQSF"
     gene            2364..3173
                     /gene="yidA"
                     /locus_tag="VGT87_001629"
     CDS             2364..3173
                     /gene="yidA"
                     /locus_tag="VGT87_001629"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEQ3814463.1"
                     /translation="MSIKLVAVDIDGTLLTDDRRITDDVFQAVQEAKAQGVHVVIATG
                     RPIAGVISLLEQLELNHKGNHVITFNGGLVQDAETGEEIVKELMTYDDYLETEFMSRK
                     LGVHMHAITKEGIYTANRNIGKYTVHESTLVNMPIFYRTPEEMTNKEIIKMMMIDEPD
                     LLDAAIKQIPQHFFDKYTIVKSTPFYLEFMPKTVSKGNAIKHLAKKLGLDMSQTMAIG
                     DAENDRAMLEVVANPVVMENGVPELKKIAKYITKSNNDSGVAHAIRKWVLN"
     gene            3173..4399
                     /locus_tag="VGT87_001630"
     CDS             3173..4399
                     /locus_tag="VGT87_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase FemX"
                     /protein_id="HEQ3814464.1"
                     /translation="MYTYKIGISPEEHDQFVLAQPQAGLLQSSKWGKVKDNWKHERIS
                     FYENGVQVAAAACLIRKLPLGFTMIYIPRGPIMDYANFELLDFVIKTLKTFGKSKRAL
                     FVKIDPSLVIKQTLEGKESKENDVALSIIAFLKKLGVEWSGRTKELEDTIQPRIQANI
                     YAKDFDFDSLPKKAKQSIRTATNKGVNVTIGGSELLDDFSALMKKTENRKGIILRGKS
                     YYQKLLGIYAGQSYITMASLDLPEQKKLLIQQLDKALAEQARLTDKSKPSKVAENQKT
                     IARLQKDLTILSEQLAAGKTRIPLAATLTLIYGESSENLYAGMDDNYRNYQAPLLTWY
                     ETAKEAFKRGCRWHNLGGVENQQDGGLYHFKARLNPTIEEFAGEFNIPVGLVSSLAIL
                     TYNLRKKLRSTTNGTN"
     gene            4386..>5317
                     /locus_tag="VGT87_001631"
     CDS             4386..>5317
                     /locus_tag="VGT87_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEQ3814465.1"
                     /translation="MALIEISQEQFDHYCHSLVRHSFIQTSEMASLMAKRGAKPQFLG
                     LEKDGELKVAAMVFSQKVAGGWRMELNAGPNTNHPEELEHFYTQLKDYAKQKDVIELI
                     LKPYDNYQSFDTDGIPISHPNTDLISLLTALGYKHDGLKTGYPEGEPVWHYVKKLEGI
                     DSSRLTHSFSKKGKALIKKANTFGIKLRQLKRDELHHFKEITEATSDRRDYLDKSLSY
                     YQDFYDSFGDSCEFMVATLNFEDYLNNLKQRQLQLATSINKVKGDLGKNPHSEKKQNR
                     LKELSSQFETFQVRISEALHFLEEYGTKDVFLAG"
BASE COUNT         1703 a         1116 c          978 g         1520 t
ORIGIN      
        1 cggaaaccga aattccgtat tgacgataca atcacggcta gccgtctgct actcccttga
       61 tataataacc attatagtaa aaacctgact aaaaaacaag aataatccct aatcagatga
      121 tttactgcta tctttttagt taatcccaac tcagctcaag atgatagctt gcaaatagca
      181 tttctgtttg agcttgaagc aaatgataat gaaggtccaa tttttgacaa gaacaatcta
      241 agtggtaatg actggtatct gtcaaaaatt gttgaacccc catcggtgta ggtagagcaa
      301 tcaggacctt cttgcccgca aaaaatttca tgattgactg agggttggaa aaacggctca
      361 tggtcagttc tgtctcattg tacttgatga caaccttttc cttgtcagtg ttttgataga
      421 tgaggtactg atagccccct ttttctcgcc actcacaatc atgaatttct tgaataatct
      481 ctgtctcatc tccaaagcga atatggttgg ttaactgtaa tttcataact cttctttcta
      541 ttctaagctc gtctgtttat tctatcaatt tctccttatt ttatcaattt ttgatataat
      601 aaaaattatg aatgaaaaag tatttcgtga ccctgttcac aactatatcc acattgataa
      661 tccattaatt tatgacctta tcaataccaa agaattccaa cgcttacgac gcatcaaaca
      721 agtccctaca acagctttca ccttccacgg cgcagaacat agccgttttt ctcactgcct
      781 aggtgtttac gaaattgccc gtcgggtgac agctattttt gaagagaaat acgctgatat
      841 ctggaataaa gatgaaagtc tggtcactat gacggctgcc ctcttacatg acattggtca
      901 tggagcctat tctcatacct ttgaggccct tttccatacg gatcacgagg cctttactca
      961 ggagataatt accaatcctg agacagaaat caatgccatt ttggtgcgtc atgctcttga
     1021 ttttccagac aaagttgcca gcgtgattaa tcatacctat cctaacaagc aagttgtgca
     1081 actgatttcc agccagattg actgcgaccg tatggattac cttttaagag attcttattt
     1141 ttctgctgcc aattatggtc agtttgattt aatgcgcatt ctacgtgtga ttcgacctgt
     1201 tgaagatggc attgtctttg aacatagtgg tatgcatgct gtcgaggact atattgtcag
     1261 tcgttttcaa atgtacatgc aggtctattt tcatcccgcc agccgtgctg ttgagctcat
     1321 cttacaaaat ctcctcaaaa gagcccaaca cttatatcct gagcaacagg cttatttcca
     1381 aaaaacagct cctggactca tccccctctt tgaaaagaag gctaatttag cagattatat
     1441 agccctagat gacggagtca tgaatactta cttccaagtc tggatggcaa gcgaagacca
     1501 tatcttatca gatttggcca gtcgctttat taatcgaaaa attctgaagt ctgttacctt
     1561 tgaccaagat tcccaaggag agttagaacg cttgcgccaa ttggtcgaat cagttggctt
     1621 tgatccagat tattacaccg gtatccatat caacttcgac ctgccttatg atatttatcg
     1681 accagagctc gaaaatccaa gaactcagat tgaaatgata caaaaagacg ggagccttgc
     1741 tgaattatct cagttatctc ctattgtcaa agcattaaca gggacaacct acggagaccg
     1801 tcgcttttat tttccaaagg aaatgttaga gctcgatgac ctctttgctc ctagcaagga
     1861 aacttttatg agttatattt ccaatgggca ttttcacttt agtcagtaaa gccctttaag
     1921 attgcagaaa aagtcttttt tgatactttt cttaggtgat gcttatggca gcgacttcct
     1981 ttggaattct atgctttatt tttgagcctg gggtctcaaa aattccgcgt aacgtcactg
     2041 ccagtattga caccactttt gccacagcaa aaagcagctg ttttggctca cgtcacacgc
     2101 agaaataaga aatcagttgt caaatcagca gagcagttca tgctttttga gcatcaaact
     2161 gggtttgtct ccattctaag attgaagaaa gagtactttc ttcaatcttt ttagttacta
     2221 cagaggcgat tgagctaaac taaaacgttt tctctcgttt ttccttaagt ttcctttccc
     2281 atcccatgac aaaagcaccg atttttagta agataggagg gagaacaatt ttaagataaa
     2341 ccacaaaaaa caaggaggat gccatgtcta tcaaactggt tgctgtcgat attgatggca
     2401 cattactcac tgatgaccgt cgaattacag atgatgtttt tcaagctgtc caagaggcta
     2461 aagcccaagg tgttcatgtg gtgattgcta ctggccgacc tattgccgga gtgatttcat
     2521 tactcgaaca attagaactc aaccacaaag gaaaccacgt tatcactttt aatggtggct
     2581 tggtgcaaga tgccgagacc ggcgaagaaa ttgttaaaga attgatgact tacgacgatt
     2641 atctggaaac ggaatttatg agccgaaaat taggagttca catgcatgcc attaccaaag
     2701 aaggtattta cactgccaat cggaatattg gaaaatacac agttcatgaa tcgactttgg
     2761 taaacatgcc tattttctat cgcactcccg aagaaatgac caataaagaa atcatcaaaa
     2821 tgatgatgat tgatgaacct gaccttttgg acgcggccat caagcaaatt cctcaacatt
     2881 tctttgataa gtacaccatc gtcaagtcaa ccccttttta tttagagttc atgccaaaaa
     2941 ccgtcagcaa gggcaatgcc atcaaacact tagctaaaaa actcgggcta gatatgtcac
     3001 aaaccatggc catcggggat gctgaaaatg atcgtgctat gttagaagtc gttgccaacc
     3061 ctgttgtcat ggaaaatggg gttcctgaat tgaaaaaaat agccaaatac atcaccaaat
     3121 ccaacaatga cagtggtgtg gcacatgcta ttcgcaagtg ggtattaaac taatgtatac
     3181 atataaaatc ggaatatctc ctgaagaaca tgatcagttt gtcttagcac agccacaagc
     3241 tggcttactt caaagttcaa agtggggaaa agtcaaagat aactggaaac acgaacgcat
     3301 cagcttttat gaaaatgggg tacaagttgc tgctgctgct tgtttaatca gaaaacttcc
     3361 tcttggtttc actatgattt atatcccacg tgggccaatc atggactacg ccaattttga
     3421 actccttgat ttcgtcatta aaacgctcaa aactttcgga aaaagcaaac gagcgctttt
     3481 tgtcaaaatt gatccaagtt tagtcatcaa acaaacttta gaaggaaaag agagcaaaga
     3541 aaatgatgtg gccctctcta ttattgcttt tctaaaaaaa cttggtgtcg aatggtctgg
     3601 cagaacaaaa gaactcgaag acactattca accaagaata caagccaaca tctacgccaa
     3661 ggattttgac tttgacagcc tgcccaaaaa agccaagcaa tccattcgta ccgctaccaa
     3721 taaaggtgtt aatgtcacga ttggtggcag tgaattgcta gacgactttt cagccttgat
     3781 gaaaaaaacc gagaatcgaa aaggcattat ccttcgaggc aaatcctact atcaaaaatt
     3841 actaggtatt tatgctggtc agtcctacat tactatggct agtttagact tacctgaaca
     3901 aaaaaaactc ttgatccaac agttggacaa agccttagca gaacaagcac gattaacaga
     3961 taagagtaaa cctagcaaag tagcagaaaa tcaaaaaacc attgcacggc tacaaaaaga
     4021 tttaaccatt ttaagcgagc agctcgctgc aggcaaaacc cgcatcccac tagccgccac
     4081 cctaaccttg atttatggcg agtcttctga aaatctttat gcgggaatgg acgataatta
     4141 tcgcaattat caagctcctc ttttaacctg gtatgaaacg gctaaagaag cttttaaaag
     4201 aggctgccgc tggcataatt taggaggcgt ggaaaatcaa caagatggtg gactctatca
     4261 tttcaaagct agactcaacc ccaccatcga agaatttgca ggtgaattca acattcctgt
     4321 cggccttgtt tcaagcttag ccatcttgac ctataactta agaaaaaaat taaggagcac
     4381 aactaatggc actaattgaa atttcacaag aacaatttga ccattactgc cacagtctcg
     4441 tccgtcattc ctttatccaa acctcagaaa tggccagctt gatggctaaa agaggggcaa
     4501 aaccacaatt tcttggactt gaaaaagatg gtgaactaaa ggttgctgct atggtattta
     4561 gccaaaaagt ggctggaggt tggcgtatgg aactcaatgc aggccctaat accaatcatc
     4621 ccgaagaatt agaacatttt tacactcaac ttaaagacta tgccaaacaa aaagacgtta
     4681 tcgaactcat tttaaaacct tacgacaatt atcagtcatt tgatactgat ggcatcccta
     4741 tttctcatcc aaacactgac cttattagcc ttttaacggc gttaggatat aaacatgacg
     4801 gtttaaaaac aggctatcct gagggtgaac ctgtttggca ttacgttaaa aagttagaag
     4861 gaattgattc ctcacgttta acccattcct tcagcaaaaa aggaaaagct ttaataaaaa
     4921 aagctaatac ttttggtatt aaattacgtc aactgaaacg agatgagtta caccatttca
     4981 aagaaatcac tgaagcaacc tctgatcgta gagattacct tgacaaatca ttatcttact
     5041 atcaggactt ttatgatagt tttggagact cttgtgaatt tatggttgca actcttaatt
     5101 ttgaagatta ccttaataac cttaaacaaa gacaactaca actagcaacc agcatcaaca
     5161 aagttaaagg agaccttggc aaaaatcctc attctgaaaa aaagcaaaat agattaaaag
     5221 aactatccag ccaattcgaa acctttcagg taagaatttc tgaagctctc cattttcttg
     5281 aagagtacgg aactaaagat gtgttccttg cgggaag
//
LOCUS       DAUCLR010000101         5308 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.102, whole genome shotgun sequence.
ACCESSION   DAUCLR010000101 DAUCLR010000000
VERSION     DAUCLR010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5308
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.102"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..124)
                     /locus_tag="VGT87_001632"
     CDS             complement(<1..124)
                     /locus_tag="VGT87_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnan synthesis protein F"
                     /protein_id="HEQ3814466.1"
                     /translation="MNRILLYVHFNKYNKISAHVYYQLEQMRSLFSKIVFISNSK"
     gene            complement(121..1128)
                     /locus_tag="VGT87_001633"
     CDS             complement(121..1128)
                     /locus_tag="VGT87_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEQ3814467.1"
                     /translation="MYKVSIICTNYNKAPWISDALDSFLSQVTDFEVEIIVIDDASTD
                     DSREILKSYQKKSSGKIKLLFNETNIGITKTWIKACLYAKGKYIARCDGDDYWTDSFK
                     LQKQVDVLEASKRSRWCNTDFDFVNSQGDLLYANAFSSGHTPLTDTYEKVLALKGMTM
                     ASTWLVDADLMRQVNQKINVDTPDDTFDIQLELFQLTQLTYIKDSTTIYRMTTDSDSR
                     PTDTPKMIHRIQKLLDTQLNYLKKYNQVDTKEVSKLLLQQDAKQEIRIHELSCFIQEL
                     QQTIVDKTKQQETREVELQNVIEEQKNQLSELRQQYHAIINSRQWKYTSKLIAFIRRK
                     K"
     gene            complement(1153..2358)
                     /locus_tag="VGT87_001634"
     CDS             complement(1153..2358)
                     /locus_tag="VGT87_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ3814468.1"
                     /translation="MINREIAVKVQHVSKTFKLPTEATKSFRTTLVNRLRGIKGFTEQ
                     HVLKDINFDVYKGDFFGIVGRNGSGKSTLLKIISQIYVPEKGQVTVDGKMVSFIELGV
                     GFNPELTGRENVYMNGAMLGFTKDEVDDMYNDIVDFAELHHFMNQKLKNYSSGMQVRL
                     AFSVAIKAQGDVLILDEVLAVGDEAFQRKCNDYFMERKDSGKTTILVTHDMGAVKKYC
                     NRAVLIEDGLVKAYGEPFDVANQYSVDNTETAEDAMNAEKISVSDIAKDLKVSLISNP
                     RITPNDTITFEVSYEVLKDDFTYIAFSLTDIDRNIWVYNDNSLDYPTQGIGRKCISYQ
                     CQLSQLNDIKLKLEVTVRDKNGQMLLFSTAEQSPQIVIQRNDIDQDDLSALDSASGLY
                     QRNGKWVFH"
     gene            complement(2358..3161)
                     /locus_tag="VGT87_001635"
     CDS             complement(2358..3161)
                     /locus_tag="VGT87_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ3814469.1"
                     /translation="MNFLTKKNRILLREMVKTDFKLRYQGSAIGYLWSILKPLMMFTI
                     MYLVFIRFLRLGGNVPHFPVALLLANVIWSFFSEATSMGMVSIVSRGDLLRKLNFSKH
                     IIVFSAVLGALINFLINLVVVLIFALINGVTISGYAYLSLFLFIELVVLVLGIALLLS
                     NVFVYYRDLAQVWEVLLQAGMYATPIIYPITFVLDSHPLAAKLLMLNPVAQMIQDFRY
                     LLIDRANVTIWQMSTNWFYIVIPYLVPFVILFIGIFVFKKNADRFAEII"
     gene            complement(3164..4096)
                     /locus_tag="VGT87_001636"
     CDS             complement(3164..4096)
                     /locus_tag="VGT87_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEQ3814470.1"
                     /translation="MNINILLSTYNGERFLAEQIQSIQRQTVNDWTLLIRDDGSTDGT
                     QDIIRTFVKEDKRIQWINEGQTENLGVIKNFYTLLKHQKADVYFFSDQDDIWLDNKLE
                     VTLLEAQKHEMAAPLLVYTDLKVVTQHLAICHDSMIKTQSGHANTSLLQELTENTVTG
                     GTMMITHALAEEWTTCDGLLMHDWYLALLASATGKLVYLDIPTELYRQHDANVLGART
                     WSKRMKNWLTPHHLVNKYWWLITSSQKQAQLLLDLPLKPNDHELVTAYVSLLDMPFTK
                     RLATLKRYGFRKNRIFHTFIFRSLVVTLFGYRRK"
     gene            complement(4086..5240)
                     /locus_tag="VGT87_001637"
     CDS             complement(4086..5240)
                     /locus_tag="VGT87_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HEQ3814471.1"
                     /translation="MQDVFIIGSRGLPAKYGGFETFVEELISHQSSKNIRYHVACLSD
                     TKHKVHFDYKGADCFYLNPPKLGPARVIAYDMMAITYALSYSDQHQIQNPIFYVLGNT
                     VGAFIAPFVKQIHNRGGRFFINPDGLEWKRSKWSKPVQAYLKFSEKQMTRQADLVISD
                     NIGIDRYLKQVYPWSKTCFIAYGTQTQPSRLTTADSKVRAYFQTFDIREKDYYLILGR
                     FVPENNYEAAIKEFMASSTKRDLVIICNHEGNAYFKQLLAETECDKDPRIKFVGTLYD
                     KELLAYIREQAYAYIHGHEVGGTNPGLLEALAHTNLNLVLGVDFNQSVAKSAALYWSK
                     QKGQLAELINQVDAGFDSDHLGKEAKAIIQEHYTWEKIVGEYEALFLNEH"
BASE COUNT         1689 a         1030 c          857 g         1732 t
ORIGIN      
        1 ctttactatt tgagataaaa acaatcttag aaaacagtga tctcatttgt tccaattgat
       61 agtaaacatg tgcacttatt ttattgtatt tgttgaagtg aacatataat aaaatacgat
      121 tcattttttt ctcctaataa aagcaatgag cttagacgtg tatttccatt gacgtgaatt
      181 aataatggca tggtattgtt gtcttaattc agatagttga tttttttgtt cttctatgac
      241 attttgcaac tccacttcac gtgtttcttg ctgctttgtt ttatctacga tagtttgttg
      301 taattcttga ataaagcaac tcaactcatg aattcgtatt tcttgcttag catcttgttg
      361 taacaatagt ttagacactt cttttgtatc tacctgatta tactttttta agtaatttaa
      421 ctgagtatca agtagttttt gaatacgatg aatcattttt ggtgtatcag taggtcttga
      481 atcagaatcg gttgtcatgc gataaatcgt cgttgagtct ttaatatagg tcagttgagt
      541 cagttgaaat aactctagtt gaatatcaaa agtatcgtct ggtgtatcta cattgatttt
      601 ttggtttact tgtctcatta aatcagcatc aactaaccat gtcgatgcca tcgtcatccc
      661 ttttaaggct aaaacttttt cgtaagtatc tgtcaaaggt gtatgaccag atgaaaaagc
      721 attagcatat aataagtcgc cttgactatt tacaaagtca aaatcagtat tacaccatcg
      781 tgaccgtttg gaagcttcta agacatcaac ttgtttttgg agtttaaaac tatccgtcca
      841 gtaatcatca ccatcacatc tagcaatata tttgcctttt gcataaaggc atgcttttat
      901 ccaagtcttt gttattccaa tattagtttc gttaaagagt aatttaattt ttcctgaaga
      961 ctttttttga taactcttta aaatctctcg tgagtcatct gtagaagcat catcaataac
     1021 aataatttca acctcaaaat ctgtcacttg gcttaaaaaa ctgtcaagtg catcagatat
     1081 ccaaggagct ttgttataat tggtgcaaat aattgaaact ttatacataa attcccttct
     1141 agctattact cattaatgaa aaacccactt cccattacgt tgatacaagc cacttgctga
     1201 atctagagct gacagatcat cttgatcaat atcgtttctt tgaatcacta tctggggtga
     1261 ttgttctgct gttgaaaaga gtagcatctg accgttctta tctcggacag ttacttctaa
     1321 ttttagtttt atatcattaa gttgagatag ttgacattgg taggaaatgc attttctacc
     1381 tataccttgt gttggataat ctaaagaatt gtcgttgtaa acccaaatgt ttctatcaat
     1441 atccgttaaa gaaaaggcaa tgtaggtaaa atcatctttt aaaacttcat aagaaacttc
     1501 aaatgtgatt gtgtcatttg gtgttattct tggatttgag attaatgaca ctttcaaatc
     1561 tttggcaata tcagatacag atattttttc agcattcatt gcatcctcag ctgtctctgt
     1621 attatctaca ctatactgat tagcaacatc aaatggttct ccatatgctt taacaagacc
     1681 atcttcaata agaactgcac ggttacaata cttcttaaca gctcccatat catgtgtcac
     1741 taaaatagtc gtcttaccac tgtcttttct ttccataaaa taatcattac attttcgctg
     1801 aaaagcttca tctcctacag caagcacctc atctaaaata agaacgtcac cttgggcttt
     1861 aatagccact gaaaatgcta aacgtacctg catgccagaa gaataatttt ttaatttttg
     1921 attcataaaa tgatgcaact ctgcaaaatc aacaatatca ttgtacatat catcaacttc
     1981 gtctttggta aatcccagca tggcgccgtt catatagacg ttttctcgtc ctgttaattc
     2041 tggattgaat cccactccaa gctctataaa agaaaccatc ttcccatcta ctgtaacctg
     2101 ccctttttct ggaacataga tctgggaaat aattttaagg agcgttgatt ttcccgagcc
     2161 gttacgccca acaatcccaa aaaaatcgcc tttataaaca tcaaagttaa tatcttttag
     2221 cacatgttgt tcagtaaatc ctttaatccc tcttaggcga ttaaccagag tcgtgcgaaa
     2281 acttttagtt gcttctgtag gcagtttaaa tgttttacta acatgctgta cttttactgc
     2341 aatttctcta ttaatcatta aataatctcc gcaaatctat cggcattttt cttaaagaca
     2401 aagatgccaa taaataatat aacaaatggt actaaatatg gaataacaat gtaaaaccaa
     2461 ttggttgaca tctgccaaat cgttacgttg gccctgtcaa tcaataaata acgaaaatct
     2521 tgaatcattt gtgctactgg atttagcatc aacaactttg ccgccaaagg gtggctatct
     2581 aaaacaaaag tgatcggata aatgattgga gtggcataca tacctgcttg taatagtact
     2641 tcccagactt gagctaagtc acgataataa acaaagacat tcgacaataa caatgcaatt
     2701 ccaagcacta aaacaactaa ttctataaaa agaaaaagag agagataagc ataccctgat
     2761 atagtcacac catttatcaa cgcaaaaatt aaaacaacaa ccaaattaat aagaaaatta
     2821 attaaagctc ctaacactgc cgaaaaaaca atgatgtgct tagaaaagtt caattttcgc
     2881 aacaagtctc cccgagatac aatagatacc attcccatgc tagttgcttc tgaaaaaaaa
     2941 gaccagatga catttgccaa taaaagcgct actggaaaat gaggtacatt tccacccagg
     3001 cgaagaaaac gaataaatac caagtacata atcgtaaaca tcataagcgg ttttaagata
     3061 gaccatagat agccaattgc gctcccttga tagcgtaatt taaaatccgt ttttaccatt
     3121 tctctcagta aaatacggtt tttctttgtt aaaaaattca ttcttacttt ctcctgtaac
     3181 caaataaggt aacaactaaa ctacgaaaaa taaaggtgtg aaagatgcga ttttttctaa
     3241 aaccgtacct tttcaaggta gctaagcgtt tggtaaaagg catatccaat aaagaaacat
     3301 aggccgttac caattcatga tcatttggtt ttaatggtaa atctaatagt aattgagcct
     3361 gtttttggct tgaagtaatc aaccaccaat atttgttgac taaatgatgg ggggttaacc
     3421 aatttttcat gcgtttagac caagttctag ctcccaacac gttagcatcg tgctgacggt
     3481 ataattctgt tggaatatca aggtaaacta atttcccagt agcagaagct agtaaagcta
     3541 aataccagtc atgcatcaat aaaccgtcac aagtggtcca ttcctcagcc agagcatgag
     3601 taatcatcat tgtgccgcct gttacggtat tttcggttaa ttcttgaagt aggcttgtgt
     3661 ttgcatgacc agattgggtc ttaatcatac tgtcatggca aatagctaaa tgttgggtta
     3721 ccactttgag gtctgtataa accaataaag gagctgccat ttcatgtttt tgagcctcca
     3781 ataaggtcac ttccagttta ttgtctaacc atatatcatc ttggtcactg aaaaaataga
     3841 catctgcttt ttggtgcttt aagagggtgt aaaaattctt aataactccc agattttctg
     3901 tctgcccctc attgatccac tgaattcgtt tgtcttcctt gacaaatgtg cgaataatat
     3961 cctgggtgcc atcagttgaa ccatcatctc taatcaataa ggtccagtcg ttaactgttt
     4021 gcctttggat actttgaatt tgttctgcta agaagcgttc tccattgtag gtggatagta
     4081 aaatattaat gttcatttaa aaataaagcc tcgtactccc ctacaatttt ttcccaagtg
     4141 taatgttctt gaataatagc tttggcttct ttgcccagat gatcactatc aaaacctgca
     4201 tcaacttggt taatcagctc tgctaattgc cctttttgct tggaccaata cagcgcagca
     4261 gacttagcca ccgactggtt aaaatcaact cctaaaacca aattaagatt ggtatgggct
     4321 agtgcttcta gaaggcctgg attagtccct cctacctcat gaccatggat ataggcataa
     4381 gcctgttctc gaatgtaggc taatagttct ttatcataaa gggtaccaac aaatttaatg
     4441 cgtgggtctt tatcacactc tgtctcagca agtaattgct taaagtaagc attgccttca
     4501 tgattacaaa taatcactaa atcacgctta gttgaggaag ccataaattc tttaatagca
     4561 gcttcgtaat tattttcagg aacaaagcgt cctaaaatga gatagtaatc cttttctcta
     4621 atatcaaagg tttgaaaata agctctgacc ttgctatctg ctgttgtcaa acgtgaaggt
     4681 tgtgtctgcg ttccataagc aataaaacat gttttagacc aaggataaac ctgtttaagg
     4741 tagcggtcaa tgccaatatt atctgaaatg accaaatcag cctgtctagt catttgcttt
     4801 tcggaaaact tcaggtaggc ctgcactggt tttgaccatt tagaccgttt ccactctaac
     4861 ccgtctggat tgataaaaaa gcgtccacct cgattgtgaa tctgcttcac aaatggcgca
     4921 ataaaagcgc ctacagtatt gcccaacaca taaaaaatag ggttctgaat ctggtgttgg
     4981 tcgctataag acagggcata ggtaattgcc atcatatcat aagcaataac tctagcagga
     5041 ccaagttttg ggggattaag gtaaaagcaa tctgccccct tataatcaaa atgaacctta
     5101 tgcttcgtat cgcttaaaca agcaacgtga taacgaatgt ttttagatga ttgatgacta
     5161 atcaactctt cgacaaaggt ttcaaagcca ccgtatttcg ctggtaaccc tctgctacca
     5221 atgataaaaa catcctgcat tgcatactac cccattctaa aatataatca gtgatattat
     5281 accacaatta gaaggctaat gcgacctt
//
LOCUS       DAUCLR010000102         5172 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.103, whole genome shotgun sequence.
ACCESSION   DAUCLR010000102 DAUCLR010000000
VERSION     DAUCLR010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5172
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.103"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(128..2392)
                     /gene="glgP"
                     /locus_tag="VGT87_001638"
     CDS             complement(128..2392)
                     /gene="glgP"
                     /locus_tag="VGT87_001638"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEQ3814472.1"
                     /translation="MTRFTEYVETKLGKSLTQASNEEIYLSLLNFVKEEASHKAKNSA
                     KRKVYYISAEFLIGKLLSNNLINLGIYKDIKEELAAAGKSIAEVEDVELEPSLGNGGL
                     GRLASCFIDSIASLGINGEGVGLNYHCGLFKQVFKHNEQEAEPNYWIEDQSWLVPTNI
                     SYDVPFKNFTLKSRLDRIDVLGYKRDTKNYLNLFDIEGVDYGLIKDGISFDKTQIAKN
                     LTLFLYPDDSDKNGELLRIYQQYFMVSNAAQLIIDEAIERGSNLHDLADYAYVQINDT
                     HPSMVIPELIRLLTEKHGFDFDEAVAVVKNMVGYTNHTILAEALEKWPTAYLNEVVPH
                     LVTIIEKLDALVRSEVSDPAVQIIDESGRVHMAHMDIHFATSVNGVAALHTEILKNSE
                     LKAFYDLYPEKFNNKTNGITFRRWLEFANQDLADYIKELIGDEYLTDATKLEKLMAFA
                     DDKAVHAKLAEIKFNNKLALKRYLKDNKDIELDEHSIIDTQIKRFHEYKRQQMNALYV
                     IHKYLEIKKGNLPKRKITVIFGGKAAPAYIIAQDIIHLILCLSELINNDPEVSPYLNV
                     HLVENYNVTVAEHLIPATDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIA
                     ELAGMENIYTFGKDSDTIINLYATASYVAKDYYDNHPAIKAAVNFIISPELLAFGNEE
                     RLDRLYKELISKDWFMTLIDLEEYIEVKEKMLADYEDQDLWMTKVVHNIAKAGFFSSD
                     RTIEQYNEDIWHSR"
     gene            complement(2427..3920)
                     /gene="malQ"
                     /locus_tag="VGT87_001639"
     CDS             complement(2427..3920)
                     /gene="malQ"
                     /locus_tag="VGT87_001639"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEQ3814473.1"
                     /translation="MNKRASGILMHISSLPGKFGIGTFGKSAFEFVDFLAETKQTYWQ
                     ILPLTTTSFGDSPYQSFSAIAGNTHFIDFELLVDDELLEATDLCDITFGTNPEAVDYA
                     QLFQVRRPLLEKAVRAFVAEQENVCKLEAFETASSWLTDFAEFMALKEYFNNKALQDW
                     DDETVIKRQEDSLNNYRELLAKKITYHKVCQYFFYQQWSALKTYANHKGIEIIGDMPI
                     YVSADSVEVWTMPELFKVDSDKKPLFIAGVPADGFSEDGQLWGNPTYNWSAHEKSNFA
                     WWIYRIQESFKLYDQLRIDHFKGFSDFWEIPAGDKTARNGHWASAPGIALFSAVREAL
                     GELPIIAENLGYIDEKAEQLLASTGFPGMKILEFGLFDITSQSIDLPHYYDRNCVAYT
                     GTHDNEVVNGWYDNLSEEQVHFVNNYLHKHADESITKAMLRTIFASVCDTAILCIQDL
                     LDKDGKSRMNMPNTIGGNWQWRMLDGELNQDHKDYLIYLTDLYGRAR"
     gene            complement(4035..5054)
                     /locus_tag="VGT87_001640"
     CDS             complement(4035..5054)
                     /locus_tag="VGT87_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEQ3814474.1"
                     /translation="MVTIKDVAQKAGVNPSTVSRVLKDNRSISMKTKEKVRKAMADLG
                     YVPNVAAQILASGLTHNIGLVFPPITTSDRLSEPFFMEILSTITNEAKESHFTVSIAT
                     GISLDDLIEQVKLMHLQKRVDGFIILYSDQDDPVKKYLMTNNLPFVIVGAPQGDTNKT
                     TYIDNDNQLMAKTAVEHLYQKGHHNILFITDDLLSEVTSERYLGYLKGCFKRSLKTKP
                     MLLFDRKDPVSVEILMETISSFKATALVVVGDVLAIRMVQLLSFYDIKVPDDMSIITF
                     NNSNYAKLIHPYLTTFDINVENLGKMSFKQLLDIINSNEQNLSQTIFVPFSLKQRESV
                     RDISH"
BASE COUNT         1588 a          991 c          881 g         1712 t
ORIGIN      
        1 tgctttacgc ttaatgtaat aattaaccta agtctgaagt aaaatcataa gacagcagaa
       61 actactgtct tataatttag tttaagcatg ttacggtacc gctaccccac attgcaatta
      121 ttatttttta tcgtgaatgc caaatatctt cgttatactg ctcaatggta cggtcagatg
      181 agaagaatcc agcttttgcg atgttatgga ccacttttgt catccataaa tcttggtctt
      241 catagtctgc taacattttt tctttcactt caatgtactc ttcaaggtca atcaaagtca
      301 tgaaccagtc ttttgaaatc aattctttat aaagacgatc aagacgttct tcgttgccaa
      361 atgctagcaa ttctggactg ataataaagt tcactgctgc tttaatagca gggtggttat
      421 catagtaatc ttttgctaca taagaagcag tcgcataaag gttgatgatg gtgtcagaat
      481 ctttaccaaa ggtatagata ttctccatgc ctgcaagctc agcgatttct acgttagcac
      541 cgtccattgt accaagtgtt aaagcaccgt taagcatgaa tttcatatta ccagttccag
      601 aagcttcttt ggatgctagt gaaatttgct cagaaatatc agttgctgga atcaagtgct
      661 ctgctactgt cacgttgtaa ttttcaacta gatgcacatt aaggtatggg cttacttcag
      721 ggtcattgtt aatcaattca gacaagcaaa ggatcaagtg aatgatgtct tgagcaatga
      781 tgtaagcagg cgctgcttta cctccaaaga taacagtgat tttacgtttt ggaaggttgc
      841 cttttttaat ttccaaatat ttgtgaatca cgtaaagagc attcatctgt tgacgtttgt
      901 actcgtggaa acgtttgatt tgggtatcaa taatagaatg ttcatcaagc tcaatgtctt
      961 tattgtcttt aaggtaacgt ttaagagcta atttgttgtt gaatttgatt tcagccaatt
     1021 tagcatgaac agctttgtca tctgcaaagg ccatcaattt ttctaatttt gttgcgtcag
     1081 tcaagtactc atcgccaata agttctttaa tgtaatcagc caagtcttgg ttagcaaatt
     1141 ctagccaacg acggaaagta atcccgttgg ttttgttgtt gaatttttct gggtaaaggt
     1201 catagaaagc ttttaattcg ctgtttttca agatttctgt gtggagtgct gctaccccat
     1261 tgacacttgt tgcaaaatga atatccatat gggccatgtg cacacgacct gattcgtcaa
     1321 taatttgaac agctggatca gacacttctg aacgaacaag agcatccaat ttttcaatga
     1381 tggttaccaa gtgtggcact acttcgttta agtaagcagt tggccatttt tcaagggctt
     1441 ctgcaagaat agtgtggtta gtgtaaccaa ccatattttt cacaacagct accgcttcat
     1501 caaagtcaaa gccatgtttt tcagtcaaga gacgaattaa ttctgggatg accattgacg
     1561 ggtgagtgtc attgatttgc acgtaagcgt agtctgcaag gtcatgaagg tttgaaccac
     1621 gttcgatagc ttcatcaatg attaattgcg ctgcatttga caccataaag tactgttggt
     1681 aaatacggag caattcccca tttttgtcag aatcgtctgg gtagaggaac aaggtcaagt
     1741 ttttagcaat ttgcgtttta tcaaatgaaa taccgtcttt gattaacccg taatcaacgc
     1801 cctcgatatc aaacaagtta aggtagtttt tagtgtcgcg tttgtaaccc aaaacatcaa
     1861 tacgatcaag acgagatttc aaggtgaagt tcttaaaagg cacatcatac gaaatgtttg
     1921 taggaaccaa ccatgactgg tcttcaatcc agtagtttgg ttcagcttct tgctcattgt
     1981 gtttgaagac ttgcttaaac aacccacaat gatagtttaa accaacccct tcaccattaa
     2041 tcccaagaga tgcaatagaa tcaatgaaac atgatgccaa acgccctaaa ccaccattac
     2101 ctagtgatgg ttctaattca acatcttcga cttctgcgat agatttgcca gcagctgcca
     2161 attcttcttt gatgtctttg taaattccta ggttaatcag gttgtttgat agtaatttac
     2221 caatcaaaaa ctctgctgag atatagtaaa ctttgcgttt agcagaattt ttagccttgt
     2281 ggctagcttc ttctttgaca aagtttaata atgatagata aatctcttca ttgctggctt
     2341 gcgtaagtga ttttcctagt ttagtttcaa catattctgt aaagcgtgtc atgtcttcct
     2401 cttttcaata atgtaacatt taaatattat cttgcgcgac cataaagatc tgttaaataa
     2461 ataaggtaat ctttgtgatc ttggttcaat tcaccgtcaa gcatacgcca ttgccaatta
     2521 cctccaattg tatttggcat attcatacga ctcttaccat ctttatctaa taaatcttgg
     2581 atgcaaagaa tggctgtatc acaaacagaa gcaaagattg ttcttagcat ggcttttgta
     2641 atactttcat ctgcatgttt gtgaagatag ttatttacaa agtgtacttg ttcttcacta
     2701 agattgtcat accatccgtt aaccacctcg ttatcatgag tccctgtgta agcgacacaa
     2761 ttacggtcat agtaatgtgg taaatctata ctttgactcg ttatatcaaa taaaccaaat
     2821 tctaatatct tcatgccagg aaagcctgtt gatgctaata gctgctctgc tttttcatca
     2881 atataaccaa ggttttctgc gatgatagga agctctccta aggcctcacg aaccgcagaa
     2941 aataacgcta tacctggggc tgaggcccaa tggccatttc tagcagtttt atcaccagca
     3001 ggaatttccc aaaaatctga gaagccttta aaatgatcga ttcttagctg atcatatagt
     3061 ttgaaacttt cttgaatacg ataaatccac catgcaaaat tagacttttc atgagcagac
     3121 caattgtagg ttggatttcc ccacagctga ccatcttcac taaagccatc tgctggtaca
     3181 cctgcaataa acaatggctt tttatcacta tctactttaa atagctcagg catcgtccaa
     3241 acttctacac tgtccgcaga aacataaata ggcatatcac caataatttc tatcccttta
     3301 tggttagcgt aagttttcaa ggctgaccat tgttggtaaa agaagtattg acagacttta
     3361 tgataggtaa tcttttttgc aagcaactca cggtaattat taagtgagtc ttcttggcgt
     3421 ttaatgacag tttcgtcgtc ccaatcttgt aaggctttat tgttaaaata ttcttttaaa
     3481 gccataaatt cagcaaaatc agttaaccag ctagaagctg tttcaaaagc ttctaattta
     3541 cacacatttt cttgttcagc aacaaaagct ctcactgctt tttctaaaag tggacgccta
     3601 acttgaaaaa gctgagcata gtctactgct tcaggatttg tgccgaatgt aatatcacac
     3661 aaatctgttg cttctaaaag ttcatcatct actagcaatt caaaatcaat gaaatgtgtg
     3721 ttcccagcaa tagctgaaaa tgactgataa ggagaatctc caaaactcgt cgttgttaaa
     3781 ggcagaattt gccaataggt ttgtttcgtc tctgctagaa aatcaacaaa ttcaaaagct
     3841 gattttccaa aagtgcctat cccaaatttc ccaggcaggg aactaatatg cattaagata
     3901 ccgcttgcac gtttgttcat gatttttctc cttgtgaaat ctattcatga tgagattata
     3961 acgcaagcgc ttgcgtaagt caaccagttt cctaaaaaaa gtaacattcc ttaaaaatgc
     4021 tacttttttt gttcttaatg actaatatca cggacgcttt ctctttgttt taaactaaaa
     4081 ggaacaaaaa tagtctgact aagattctgt tcattggaat tgataatatc aagtaattgc
     4141 ttaaaagaca tttttccaag attttcaaca tttatatcaa atgtcgttaa ataagggtga
     4201 attaatttag cgtaattaga attattaaaa gtgataattg acatatcatc tggcactttt
     4261 atatcataaa aagataataa ctgtaccata cgaatagcca gaacatcacc aacaacaacc
     4321 aaggcagttg ctttaaagga agatatagtt tccatcaata tttctacgct aacaggatcc
     4381 ttacgatcaa ataacagcat tggcttcgtt tttaaagatc gcttaaaaca acccttcaaa
     4441 tagccaagat aacgctcaga agttacttct gatagtaaat catctgttat aaataatatg
     4501 ttgtgatggc ctttttgata aaggtgttct actgctgttt tagccattag ctgattatca
     4561 ttatcaatat aagtggtttt attagtgtca ccttgtggag cacctacaat gacaaatggt
     4621 aagttatttg tcatcaaata ttttttgaca ggatcatctt gatcagaata aagaataata
     4681 aaaccatcaa ctcgtttttg aagatgcatc agtttcactt gctctatcag gtcatcaaga
     4741 gatatacctg tagcaatcga cacagtaaaa tgagattctt tagcttcgtt agtaatagtc
     4801 gacaaaattt ccataaagaa aggttcactt aatctatcgc tagttgtaat aggaggaaaa
     4861 acaagtccta tattgtgtgt caaaccactc gctagaatct gtgctgcgac atttggaaca
     4921 tatcccaaat ctgccatcgc ttttctaacc ttctcttttg ttttcataga aatagatcga
     4981 ttatctttta ataccctact caccgtcgaa gggttaacac ctgccttttg agcgacatct
     5041 tttattgtta ccatattaac ctcgttacta tatttaccaa agataaacct tatcatgggt
     5101 ttgtcatgta tttcgtttcc attatacaat tatctttcaa aaaatcaact tcaatcaaga
     5161 ttcccttgtc at
//
LOCUS       DAUCLR010000103         4946 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.104, whole genome shotgun sequence.
ACCESSION   DAUCLR010000103 DAUCLR010000000
VERSION     DAUCLR010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.104"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..1441
                     /locus_tag="VGT87_001641"
     rRNA            <1..1441
                     /locus_tag="VGT87_001641"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1493..1565
                     /locus_tag="VGT87_001642"
     tRNA            1493..1565
                     /locus_tag="VGT87_001642"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1526..1528,aa:Ala,seq:tgc)
     gene            1864..4766
                     /locus_tag="VGT87_001643"
     rRNA            1864..4766
                     /locus_tag="VGT87_001643"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            4852..>4946
                     /gene="rrf"
                     /locus_tag="VGT87_001644"
     rRNA            4852..>4946
                     /gene="rrf"
                     /locus_tag="VGT87_001644"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1393 a         1019 c         1420 g         1114 t
ORIGIN      
        1 gggtgagtaa cgcgtaggta acctacctca tagcggggga taactattgg aaacgatagc
       61 taataccgca taagagagac taacgcatgt tagtaattta aaaggggcaa ttgctccact
      121 atgagatgga cctgcgttgt attagctagt tggtgaggta aaggctcacc aaggcgacga
      181 tacatagccg acctgagagg gtgatcggcc acactgggac tgagacacgg cccagactcc
      241 tacgggaggc agcagtaggg aatcttcggc aatgggggca accctgaccg agcaacgccg
      301 cgtgagtgaa gaaggttttc ggatcgtaaa gctctgttgt tagagaagaa tgatggtggg
      361 agtggaaaat ccaccaagtg acggtaacta accagaaagg gacggctaac tacgtgccag
      421 cagccgcggt aatacgtagg tcccgagcgt tgtccggatt tattgggcgt aaagcgagcg
      481 caggcggttt tttaagtctg aagttaaagg cattggctca accaatgtac gctttggaaa
      541 ctggagaact tgagtgcaga aggggagagt ggaattccat gtgtagcggt gaaatgcgta
      601 gatatatgga ggaacaccgg tggcgaaagc ggctctctgg tctgtaactg acgctgaggc
      661 tcgaaagcgt ggggagcaaa caggattaga taccctggta gtccacgccg taaacgatga
      721 gtgctaggtg ttaggccctt tccggggctt agtgccggag ctaacgcatt aagcactccg
      781 cctggggagt acgaccgcaa ggttgaaact caaaggaatt gacgggggcc cgcacaagcg
      841 gtggagcatg tggtttaatt cgaagcaacg cgaagaacct taccaggtct tgacatcccg
      901 atgcccgctc tagagataga gttttacttc ggtacatcgg tgacaggtgg tgcatggttg
      961 tcgtcagctc gtgtcgtgag atgttgggtt aagtcccgca acgagcgcaa cccctattgt
     1021 tagttgccat cattaagttg ggcactctag cgagactgcc ggtaataaac cggaggaagg
     1081 tggggatgac gtcaaatcat catgcccctt atgacctggg ctacacacgt gctacaatgg
     1141 ttggtacaac gagtcgcaag ccggtgacgg caagctaatc tcttaaagcc aatctcagtt
     1201 cggattgtag gctgcaactc gcctacatga agtcggaatc gctagtaatc gcggatcagc
     1261 acgccgcggt gaatacgttc ccgggccttg tacacaccgc ccgtcacacc acgagagttt
     1321 gtaacacccg aagtcggtga ggtaacctat taggagccag ccgcctaagg tgggatagat
     1381 gattggggtg aagtcgtaac aaggtagccg tatcggaagg tgcggctgga tcacctcctt
     1441 tctaaggaaa tggaacacgt ttatcgtctt atttagtttt gagaggtctt gtggggcctt
     1501 agctcagctg ggagagcgcc tgctttgcac gcaggaggtc agcggttcga tcccgctagg
     1561 ctccatcagg atacaatcct actaaactta atacaagtga agttgaacac gcaactcact
     1621 tcctaggaaa atagacaatc ttcccttgtg tgcaaggccc acatggtcag attcctaatt
     1681 ttctacagaa gtttcgctaa agcgagcgtt gcttagtatc ctatataata gtccattgaa
     1741 aattgaatat ctatatcaaa ttccacgatc tagaaataga ttgtagaaaa gtaacaagaa
     1801 aataaaccga aaacgctgtg actatttaat aagttttcta gtttaaaaaa actaggttaa
     1861 taaggttaag ttaataaggg cgcacggtgg atgccttggc actagaagcc gaagaaggac
     1921 gtgacaaacg acgaaatgct ttggggagct gtaagtaagc gctgatccag agatgtccga
     1981 atgggggaac ccactaacta atggttagta tccataactg ttaaggttat gagaaggaag
     2041 acgcagtgaa ctgaaacatc taagtagctg caggaagaga aagcaaacgc gattgcctta
     2101 gtagcggcga gcgaaacggc aagagggcaa accgaagtgt ttacacttcg gggttgtagg
     2161 actgcgacgt gggactttaa aaggatagaa gaattacctg ggaaggtaag ccaaagagag
     2221 taaaagcctc gtatttaaaa ttcttttgag ccctagcagt atcctgagta cggcgagaca
     2281 cgtgaaatct cgttggaatc tgggaggacc atctcccaac cctaaatact ctctagtgac
     2341 cgatagtgaa ccagtaccgt gagggaaagg tgaaaagcac cccgggaggg gagtgaaata
     2401 gaacctgaaa ccgtgtgcct acaacaagtt cgagcccgtt aatgggtgag agcgtgcctt
     2461 ttgtagaatg aaccggcgag ttacgatatg atgcgaggtt aagttgaaga gacggagccg
     2521 tagggaaacc gagtcttaat agggcgattt agtatcatgt tgtagacccg aaaccatgtg
     2581 acctacccat gagcaggttg aaggtgaggt aaaactcact ggaggaccga accagggcac
     2641 gttgaaaagt gcttggatga cttgtgggta gcggagaaat tccaaacgaa cttggagata
     2701 gctggttctc tccgaaatag ctttagggct agcgtcgatg ttaagtctct tggaggtaga
     2761 gcactgtttg ggtgaggggt ccatctcgga ttaccaatct cagataaact ccgaatgcca
     2821 acgagatatc atcggcagtc agactgcgag tgctaagatc cgtagtcgaa agggaaacag
     2881 cccagaccac cagctaaggt cccaaaataa ctgttaagtg gaaaaggatg tggggttgca
     2941 cagacaacta ggatgttagc ttagaagcag ctattcattc aaagagtgcg taatagctca
     3001 ctagtcgagt gaccctgcgc cgaaaatgta ccggggctaa aacagtttac cgaagctgtg
     3061 gatgacacaa aagtgtcatg gtaggagagc gttctatgtg tgaagaaggt gtaccgtgag
     3121 gagcgctgga acgcatagaa gtgagaatgc cggtatgagt agcgaaagac aggtgagaat
     3181 cctgtccacc gtaagactaa ggtttccagg ggaaggctcg tccgccctgg gttagtcggg
     3241 acctaaggag agaccgaaag gtgtatccga tggccaacag gttgatattc ctgtactaga
     3301 gtatatagtg atggagggac gcagtaggct aactaaaccg gacgattgga agagtccggc
     3361 taagcagtga ggtgtaagat gagtcaaatg cttatcttta taacattgag ctgtgatggg
     3421 gagcgaattt tagtagcgaa gttagtgatg tcacactgcc aagaaaagct tctagcgttt
     3481 aatgatactc tacccgtacc gcaaaccgac acaggtagtc gaggcgagta gcctcaggtg
     3541 atcgagagaa ctctcgttaa ggaactcggc aaaatgaccc cgtaacttcg ggagaagggg
     3601 tgctgactta ggtcagccgc agtgaatagg cccaagcaac tgtttatcaa aaacacagct
     3661 ctctgctaaa tcgtaagatg atgtataggg ggtgacgcct gcccggtgct ggaaggttaa
     3721 gaggagggtt tagcgcaagc gaagatctga attgaagccc cagtaaacgg cggccgtaac
     3781 tataacggtc ctaaggtagc gaaattcctt gtcgggtaag ttccgacccg cacgaaaggc
     3841 gtaatgattt gggcactgtc tcaacgagag actcggtgaa attttagtac ctgtgaagat
     3901 gcaggttacc cgcgacagga cggaaagacc ccatggagct ttactgcagt ttgatattga
     3961 gtatctgtac cacatgtaca ggataggtag gagccattga ctttgggacg ccagtttcga
     4021 atgaggcgtt gttgggatac tacccttgtg ttatggctac tctaacccag ataggttatc
     4081 cctatcggag acagtgtctg acgggcagtt tgactggggc ggtcgcctcc taaagagtaa
     4141 cggaggcgcc caaaggttcc ctcagattgg ttggaaatca atcgcagagt gtaaaggtat
     4201 aagggagctt gactgcgaga gctacaactc gagcagggac gaaagtcggg cttagtgatc
     4261 cggtggtacc gaatggaagg gccatcgctc aacggataaa agctaccctg gggataacag
     4321 gcttatctcc cccaagagtt cacatcgacg gggaggtttg gcacctcgat gtcggctcgt
     4381 cgcatcctgg ggctgtagtc ggtcccaagg gttgggctgt tcgcccatta aagcggcacg
     4441 cgagctgggt tcagaacgtc gtgagacagt tcggtcccta tccgtcgcgg gcgtaggaaa
     4501 tttgagagga tctgctccta gtacgagagg accagagtgg acttaccgct ggtgtaccag
     4561 ttgttttgcc aaaggcatcg ctgggtagct atgtagggaa gggataagcg ctgaaagcat
     4621 ctaagtgcga agcccccctc aagatgagat ttcccatgat tttatatcag taagagccct
     4681 gagagatgat caggtagata ggttaggagt gtaagtgtag cgatacatgt agcggactaa
     4741 tactaatagc tcgaggactt atccaaaaag aaatattgac aacgttacgg attcttgtta
     4801 gactatagat attcaatttt gagtgggtta tgaaaatagc acatttacaa gttaagtgac
     4861 gatagcctag gagatacacc tgtacccatg ccgaacacag cagttaagcc ctagaacgcc
     4921 tgatgtagtt gggggttgcc ccctgt
//
LOCUS       DAUCLR010000104         4831 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.105, whole genome shotgun sequence.
ACCESSION   DAUCLR010000104 DAUCLR010000000
VERSION     DAUCLR010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.105"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(6..860)
                     /gene="rfbD"
                     /locus_tag="VGT87_001645"
     CDS             complement(6..860)
                     /gene="rfbD"
                     /locus_tag="VGT87_001645"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEQ3814475.1"
                     /translation="MILITGSNGQLGTELRYLLDERGVDYVAVDVAEMDITNEDKVEA
                     VFAQVKPTLVYHCAAYTAVDAAEDEGKALNEAINVTGSENIAKACGKYGATLVYISTD
                     YVFDGNKPVGQEWVETDRPDPKTEYGRTKRLGELAVERYAEYFYIIRTAWVFGNYGKN
                     FVFTMEQLAENHSRLTVVNDQHGRPTWTRTLAEFMCYLTENQKAFGYYHLSNDAKEDT
                     TWYDFAKEILKDKAVEVVPVDSSAFPAKAKRPLNSTMNLDKAKATGFVIPTWQEALKA
                     FYQQGLKK"
     gene            complement(998..1336)
                     /locus_tag="VGT87_001646"
     CDS             complement(998..1336)
                     /locus_tag="VGT87_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEQ3814476.1"
                     /translation="MSDTPKYTQDQVIAIKNRILEALETVIDPELGIDVVNLGLIYEI
                     RFNDNGYTEIDMTLTTMGCPLADLLTDYIHDALQDVPEVTKTEVKLVWYPAWTVDKMS
                     RYARIALGIR"
     gene            complement(1573..2682)
                     /gene="rpoD"
                     /locus_tag="VGT87_001647"
     CDS             complement(1573..2682)
                     /gene="rpoD"
                     /locus_tag="VGT87_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEQ3814477.1"
                     /translation="MTKQKEITTFNVQVAEFIRHHKKEGTAIDDDVTEKLVIPFALDA
                     DQIDDLLERLTDGGISITDKEGNPSSKYIVEEPKPEELTDEELIGSNSAKVNDPVRMY
                     LKEIGVVPLLTSEEEKELAVAVAKGDLMAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMEMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRVVLREQLDEVLDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRHPSRSKQLRDFIED"
     gene            complement(2691..4505)
                     /gene="dnaG"
                     /locus_tag="VGT87_001648"
     CDS             complement(2691..4505)
                     /gene="dnaG"
                     /locus_tag="VGT87_001648"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEQ3814478.1"
                     /translation="MGFLWGGDDLAIDKEMISQVKNSVNIVDVIGEVVKLSRSGRHYL
                     GLCPFHKEKTPSFNVVEDRQFFHCFGCGKSGDVFKFIEEYRQVPFLESVQIIADKTGM
                     SLNIPPSQAVLASQHKHPNHALMTLHEDAAKFYHAVLMTTTIGQEARKYLYQRGLDDQ
                     LIEHFNIGLAPDESDYLYQALSKKYEEGQLVASGLFNLSDQSNTIYDAFRNRIMFPLS
                     DDRGHIIAFSGRIWTAADTEKRQAKYKNSRGTVLFNKSYELYHLDKARPVIAKTHEVF
                     LMEGFMDVIAAYRSGYENAVASMGTALTQEHVNHLKQVTKKVVLIYDGDDAGQHAIAK
                     SLELLKDFVVEIVRMPNKMDPDEFVQRHSPEAFADLLKQSRISSVEFFIDYLKPTNVD
                     NLQSQIVYVEKMAPLIAQSPSITAQHSYINKIADLLPNFDYFQVEQSVNALRIQDRQK
                     RQGQIAQAVSNLVTLPMPKSLTAIAKTESHLMHRLLHHDYLLNEFRHRDDFYFDTSTL
                     ELLYQRLKQQGHITSYDLSEMSEEVNRAYYNVLEENLPKEVALGEIDDILSKRAKLLA
                     ERDLHKQGKKVRESSNKGDHQAALEVLEHFIAQKRKME"
     gene            4635..>4831
                     /locus_tag="VGT87_001649"
     CDS             4635..>4831
                     /locus_tag="VGT87_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HEQ3814479.1"
                     /translation="MVKELKAFLFRGNIIELAVAVIIGGAFGAIVTSFVNDIITPLIL
                     NPALKAANVENITQLSWNGVK"
BASE COUNT         1381 a         1001 c          854 g         1595 t
ORIGIN      
        1 aatacttact ttttcagtcc ttgttggtaa aatgccttca aggcttcttg ccatgttgga
       61 ataacaaacc ctgttgcttt tgccttatca agattcatgg ttgaatttaa aggacgtttg
      121 gcttttgctg ggaaagctga tgaatcaact ggcaccactt caacagcctt gtcttttaag
      181 atttctttgg caaaatcata ccaagtggtg tcttccttag catcatttga caagtgatag
      241 taaccaaagg ctttttgatt ctctgtcaaa taacacatga attctgccaa ggttcttgtc
      301 cacgttggcc gaccgtgttg gtcattgaca acagtcaaac gggagtgatt ttccgctaac
      361 tgctccatgg taaagacaaa gtttttacca taatttccaa atacccaagc agtacggata
      421 atataaaaat actctgcgta tcgttcaaca gctagttctc ctaaacgttt tgtacggcca
      481 tattctgtct tagggtctgg acgatctgtt tctacccatt cttgaccgac tggtttattg
      541 ccatcaaata cgtagtctgt tgaaatgtaa acaagggtag ccccatattt tccacaagcc
      601 ttggcaatat tttctgagcc tgttacatta atagcctcat tcaaggcttt tccttcgtct
      661 tctgctgcat caactgccgt ataagcagca caatgataga ccaatgttgg cttgacctgc
      721 gcaaaaacag cttcgacttt gtcttcattt gtgatgtcca tttctgcgac atcaacggct
      781 acataatcaa cacctcgttc atctaataag tatcgaagtt ctgtccctaa ttgaccattg
      841 cttcctgtaa ttaaaatcat cagttctcct ttttgccata ggtagaacac ttttctcagt
      901 atcttagtgt tctctaacat taaccaaagg ctttcacctg tatttccttt tattctacat
      961 caaaaaaggg aatatttcat ccctttacta tcaatgatta gcgaataccc agcgcaatcc
     1021 tagcatagcg gctcatttta tcaaccgtcc aagcagggta ccagactaat ttgacctcag
     1081 tcttagtgac ctctggcaca tcttgcaggg catcatggat gtaatctgtc aacaagtctg
     1141 ccaaaggaca acccattgtt gtgagtgtca tatcaatctc tgtataaccg ttatcattaa
     1201 accgaatttc ataaatcaag cctagattaa caacatcaat acctagctcg ggatcaataa
     1261 cagtttctaa agcttcaaga attctattct tgatagctat cacctgatct tgtgtatatt
     1321 ttggtgtatc agacatggct ttcctttcat tttttgaata tgtcgtagat aagtaaaaaa
     1381 accactaaag ctttgcccat ttagaggaaa ttgccctctg ggcttgttta agatattaag
     1441 tccaacctta aaaatgccca ccacattcgg tccgatttct aatttgtagg tgtaccgctg
     1501 ataagtgcca cttgggattt tcgctccttt cttatttcaa aggaacgacc taaaatcgtc
     1561 cactggacga ttttagtcct ctataaagtc tcttaattgt ttactacgac taggatggcg
     1621 aagttttcta agggctttag cttcaatctg tcgaatgcgc tcacgagtaa cgttaaaaac
     1681 tttacctacg tcctctaacg tacgcatttt accatcgtca aggccaaagc gtaggcgaag
     1741 aacgttctct tcacggtcag taagcgtatc taaaacttca tccaattgct cacgaagaac
     1801 aacacgcgtc gtataatcta ctggattttc aataacttca tcttcgataa aatcacctaa
     1861 gtgactatca tcttcttccc caattggtgt ttctaaagaa acaggttctt gagcaatttt
     1921 tagaatttca cgaaccttgt ctggagtcat ttccatacgt tctgcgattt gttctggcgt
     1981 tggatcttga ccaagttctt gaagaaggtt acgttgttca cggactaatt tgttaatcgt
     2041 ttcaaccata tgaacaggga tacgaatcgt acgtgcttga tctgcaattg cgcgtgttat
     2101 agcttgacga atccaccatg ttgcataagt tgagaatttg aatccttttg aataatcaaa
     2161 tttatcaaca gctttcatga gtcccatgtt accttcttga attaaatcaa gaaattgcat
     2221 tccacgtcca acataacgtt ttgcaattga gacaacaaga cgaaggtttg cttccgctaa
     2281 acgttgttta gccattaaat ctcccttggc aaccgcaaca gccaattctt tttcctcttc
     2341 acttgttaaa agcggtacca caccaatttc tttgaggtac atgcggacag ggtcgtttac
     2401 tttagctgaa ttactaccaa ttaattcttc atcagttaat tcttctggtt ttggttcttc
     2461 aacaatgtat tttgatgatg ggttaccttc tttatccgta atcgagatac caccatctgt
     2521 taaccgctca agtaaatcat caatctgatc agcatccaaa gcaaatggaa tgacaagttt
     2581 ttcggtcacg tcatcatcaa tcgctgttcc ttcttttttg tgatggcgaa taaactccgc
     2641 aacttgaacg ttaaaagttg ttatttcttt ttgttttgtc ataatttcct ctattccatt
     2701 tttcgtttct gcgcaataaa atgttctagt acttctagag ccgcttgatg atcgcctttg
     2761 ttactagatt ctctaacttt tttcccttgt ttgtgaagat cgcgctctgc taaaagtttg
     2821 gcacgtttgg ataaaatatc atcaatctca ccaagagcta cttctttggg aaggttttct
     2881 tctaaaacat tgtaataagc acgattaact tcctctgaca tctctgacaa atcataagat
     2941 gtaatatgtc cttgttgctt cagccgttga taaagtaatt ctaaggtaga ggtatcaaaa
     3001 taaaaatcat cacgatgtcg aaattcattt aataaatagt catgatgtaa gagccgatgc
     3061 atgagatgac tttctgtctt agcaatagct gtcaaacttt ttggcattgg taaggtcaca
     3121 agattgctga cggcttgagc tatttgacct tgacgctttt gcctatcttg aatccttaat
     3181 gcatttactg attgttctac ttgaaaatag tcaaagtttg gcaacaaatc agcaatcttg
     3241 ttaatatacg aatgttgagc tgtgatggat ggtgattgag caatcaatgg tgccattttc
     3301 tccacataaa caatttgtga ttgcaaattg tctacattag taggttttag gtaatcaata
     3361 aaaaattcaa cactactgat ccgtgactgc ttaagcaaat ctgcaaatgc ttctggggaa
     3421 tgccgttgta caaattcgtc aggatccatt ttattgggca ttctgacaat ttcgacaaca
     3481 aaatctttaa gcaattctag tgattttgca atagcatgtt gtccagcatc gtcaccatca
     3541 taaatcaaaa caactttttt agtgacttgc ttaaggtgat tgacatgttc ttgagtcaat
     3601 gccgtcccca ttgaagcaac ggcattttca tagccggaac ggtaagcggc aatcacgtcc
     3661 ataaaccctt ccattagaaa tacttcatgg gttttggcaa taacaggcct tgccttgtcc
     3721 agatgataca attcataaga tttgttaaaa agaactgttc ctcttgaatt tttatacttg
     3781 gcctgtctct tttctgtatc agctgccgtc cagatacgtc ctgaaaaggc aataatatgc
     3841 cctcggtcat ctgataaggg aaacatgata cgatttcgaa aggcgtcgta aatggtatta
     3901 gattgatcgg acaagttaaa caatcctgaa gcaaccaatt gaccttcctc gtatttttta
     3961 gaaagagctt gataaagata atctgactca tctggggcta aaccaatatt gaaatgctca
     4021 attaattggt catccaagcc tctctggtaa aggtacttcc tagcttcttg accaatggta
     4081 gtggtcatca aaactgcatg gtaaaattta gcagcatcct catgaagtgt catcaaagcg
     4141 tgattagggt gcttgtgttg gctagcaagt actgcctgac ttggcggtat attaagcgac
     4201 ataccagtct tatcggcaat aatctgaaca ctttctaaga aggggacttg gcggtattcc
     4261 tcaataaatt taaaaacatc ccctgatttt ccacagccaa agcagtgaaa aaattgtctg
     4321 tcttcaacaa cattaaaaga gggtgtcttt tccttatgaa atgggcaaag cccgagataa
     4381 tgccgccctg atcgggaaag tttgaccact tctccaatga catcgacaat attaacgcta
     4441 ttttttactt gggaaatcat ttctttgtca attgccaaat cgtcacctcc ccataaaaat
     4501 cccattttag ttttacgttt tattatacca ccgaaactct tttttgtaaa tttaaaaact
     4561 gaagtgattc cattggattt tgtttactaa ttatgctatg cttaacttag agatattaaa
     4621 aggggggata tttaatggtt aaagaattaa aagcattttt gtttagagga aacattattg
     4681 aacttgctgt agcagttatc atcgggggag cttttggtgc tattgtcact tcttttgtga
     4741 acgatattat cacaccgctt attttaaacc cagctctgaa agctgcaaat gttgaaaaca
     4801 tcactcaatt gtcatggaat ggtgttaaat a
//
LOCUS       DAUCLR010000105         4717 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.106, whole genome shotgun sequence.
ACCESSION   DAUCLR010000105 DAUCLR010000000
VERSION     DAUCLR010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.106"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(143..973)
                     /locus_tag="VGT87_001650"
     CDS             complement(143..973)
                     /locus_tag="VGT87_001650"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEQ3814480.1"
                     /translation="MVLDFIEILKVIFLGIVEGITEWLPISSTGHMILADKFITLNMS
                     EAFKEMFFVVIQLGAILAVVVMFWNKMFPFQLKDKSQPLVKKDTFSLWFKVAVACIPS
                     GIMGILFDDYLDAYLQTPIVISIMLIFYGLLFILIENWNKKRTPTTMALSDISYKTAI
                     LIGAFQVLSLIPGTSRSGATIIGALLIGVSRVAAAEFTFFLAVPTMLGASAFKLLKFG
                     FDFTSAELLALVLGMAVAFAVSVLVIKFLMNFIKKHDFKVFGWYRIVLGILVVLITVM
                     "
     gene            complement(973..1722)
                     /locus_tag="VGT87_001651"
     CDS             complement(973..1722)
                     /locus_tag="VGT87_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ3814481.1"
                     /translation="MLNLISCELLKLKRSKMVLISVAGVLSTPLLMLIEALQTHFDKP
                     EIIFTLSDIYSDSVLYIMLLVNIMIYVAIAAYLFSREYTESTLKTILPIPISRTKLLI
                     GKFCTLLLWIVMLTLVTWAGIFIACGLYHAVFTLEGYSLLVAIVWLPKFLFGSILMFL
                     TVSPFVFVAMKTKGFVAPMIGSAVIVMGSAALSNQEWGALYPWTATYFLVQGKLQSTG
                     YPTLLSVSIIILVSAVGFLMTFHHFKKEDLK"
     gene            complement(1715..2632)
                     /locus_tag="VGT87_001652"
     CDS             complement(1715..2632)
                     /locus_tag="VGT87_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEQ3814482.1"
                     /translation="MDYIIETENLTKRYGTATVVDKVNLHVPTGKIYGLLGRNGAGKT
                     TAMKMMLQLALPTDGTVRLFGTNYKENIHTLYSKVGSIIETPGFYSNLTGYENLQILA
                     KLRGGVSKSGVEKALEVVGLHKEKRKVFSDYSLGMKQRLGIAAAIMHEPELLILDEPI
                     NGLDPIGIVEIRSFLSELSHNHGITIFISSHVLSEIEQIADIIGVMHEGHLVEEVNIS
                     ELHKRNRKYIQFDLSDSEIAGKILESHYHMTDFTVQDGTVRIYDFSQSVGEINREFAR
                     NGLLVTRIDDSEENLEDYFSKLIGGGGIA"
     gene            complement(2815..3429)
                     /locus_tag="VGT87_001653"
     CDS             complement(2815..3429)
                     /locus_tag="VGT87_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ3814483.1"
                     /translation="MEFNEKLQQLRTGKNLTQEQLAEQLYVSRTAISKWESGKGYPNI
                     ESLKCISKFFSVTIDELLSGEELITLAETENRSNLKKIYNYIYGILDMMAVAFIFLPL
                     YGNSVGGYVYAVNLLSFTATTPFNLAVYWSAFAALIVIGIAKIILTHLDKETWGGIAT
                     KCSLGLTALAICFLAAAREPYITALMFLLFVAKIVVWIKQNGTK"
     gene            complement(3419..3745)
                     /locus_tag="VGT87_001654"
     CDS             complement(3419..3745)
                     /locus_tag="VGT87_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEQ3814484.1"
                     /translation="MIFERSMILCLVVYNFVLVERNFLLFVIPYIIIIYSLRKEVDIM
                     DYISVKAASEKWGISERRIQKLCEENRIDGTEKFGRAWMIPKDAEKPVDGRMKTRNKQ
                     EENHGI"
     gene            3782..3946
                     /locus_tag="VGT87_001655"
     CDS             3782..3946
                     /locus_tag="VGT87_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ3814485.1"
                     /translation="MYQDERKLDFKPLGIAIKKAREAKGWTQEYLAQLVDLTPRSIMG
                     SVAKFPISSF"
     gene            3985..4665
                     /locus_tag="VGT87_001656"
     CDS             3985..4665
                     /locus_tag="VGT87_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISEnfa1 family transposase"
                     /protein_id="HEQ3814486.1"
                     /translation="MTQFKGKQFQKDVIIVAVGYYLRYNLSYREVQEILYDRGIHVCH
                     TTIYRWVQEYGKILYQIWKKKNKQSFYSWKMDETYIKIKGKWHYLYRAIDVDGLTLDI
                     WLRKKRDTQAAYAFLKRLKNQFGEPKVLVTDKAPSIKSAFRKLQKNGLYITTEHRTIK
                     YLNNLIEQDHRPIKRRNKFYQSLRTASTTIKGMEAIRGIYKKSRKEGSLFGFSVCTEI
                     KGLLGIPA"
BASE COUNT         1512 a          972 c          846 g         1387 t
ORIGIN      
        1 attggtttgt gtaaacgcaa agaaacggcg gctctgaaaa tcaaagccac cgtttcatag
       61 gcgtgaaaat atgtctgctg tacgacggct tttttctttt gccgtcgtgc ggcagattat
      121 tgcttatgga attgtttttc tatcacatta ctgttataag tacaacaagt ataccaagca
      181 caattcgata ccaaccaaac accttaaaat catgtttttt gataaagttc attaggaatt
      241 taattactaa gacagatacc gcaaatgcca cagccatacc gagaacaaga gcgagcagtt
      301 ctgcacttgt aaaatcaaac ccgaatttta acagcttaaa agcacttgct ccaagcatag
      361 taggtactgc gaggaaaaaa gtaaactctg ctgctgccac tcgtgaaact cctatgagta
      421 aagcacctat aatcgttgct cccgaccttg atgtgccggg tataagtgat aacacttgaa
      481 atgcccctat caagattgct gttttatagc tgatgtcaga aagtgccata gtagtaggag
      541 tacgcttttt attccaattt tctatgagaa tgaataacaa accataaaag attaacatga
      601 ttgaaatcac aatgggggtt tggaggtaag catccaaata atcatcaaat aaaatcccca
      661 taatacctga cggtatacaa gccactgcaa ccttgaacca caacgagaaa gtatcctttt
      721 taacaagtgg ctgcgattta tcctttagct gaaatggaaa catcttgttc caaaacatta
      781 caaccactgc gagtatagct ccaagttgaa taacaacaaa aaacatttct ttaaatgctt
      841 cgctcatatt aagtgtgata aatttgtccg caagaatcat atgtcctgtg ctgctgatag
      901 gtagccactc agtaataccc tcaacaattc caagaaaaat aacttttaaa atttctataa
      961 aatcaagtac cattatttca aatcctcctt tttaaaatga tgaaaggtca taagaaaacc
     1021 aactgctgat acaagaataa taatagatac agacagcagt gtaggatagc cggtactctg
     1081 aagtttaccc tgcaccaaga aataggtggc tgtccacgga tacaacgctc cccattcttg
     1141 atttgaaagt gcggcacttc ccatgacaat cacggcagag ccaatcatcg gggcgacaaa
     1201 tccttttgtt ttcatagcaa caaacacaaa aggagaaact gttaaaaaca tcaggatact
     1261 gccaaacaaa aacttgggga gccatactat tgccactagt aagctatatc cctccaatgt
     1321 aaagacagcg tgatatagcc cacaagcgat aaatatacct gcccatgtta caagggtgag
     1381 cataacaatc caaagaagca gggtgcaaaa ttttccaatt aatagttttg tccttgaaat
     1441 gggtatgggc aatatggttt tgagtgtgct ttctgtgtac tctctgctaa acaagtaagc
     1501 tgcaattgcc acatatatca taatgtttac cagcagcatg atatacagta cactgtcgct
     1561 gtatatgtca gacaaggtaa agataatctc cggcttatca aaatgtgttt gcaaggcttc
     1621 tattaacatc aaaagtgggg tagataatac ccctgccaca ctaattagca ccatttttga
     1681 acgctttagt tttaataatt cacaagaaat aagattaagc aataccgcca cctccaatca
     1741 gtttagaaaa gtagtcctct aagttttcct cactatcatc tatccttgtc acgagcagtc
     1801 catttcgtgc aaattctcga ttgatttctc caacactttg gctaaagtca taaattctca
     1861 ccgtaccgtc ctgcactgta aaatccgtca tgtggtagtg gctttctaaa atctttccag
     1921 ctatctcact gtcagataaa tcaaattgaa tgtattttcg attccttttg tgaagctcgg
     1981 atatattcac ttcctctact aaatgcccct cgtgcataac gccgataata tctgctatct
     2041 gttcaatctc gcttaaaaca tggcttgaga taaaaatggt aatgccatga ttgtgactaa
     2101 gttcagataa aaatgagcgt atttcaacta ttccaatggg gtcaagtccg ttaatcggtt
     2161 cgtcaagtat taaaagctcc ggctcgtgca taatggcggc ggcaatacca agccgttgct
     2221 tcattccgag ggaataatcg gaaaagactt ttcttttctc tttatgcagc cccacaactt
     2281 caagagcttt ttctactcca cttttagata ctccccctcg cagtttagcg agaatttgca
     2341 aattctcata ccctgttaaa ttactgtaaa atcccggtgt ttcgataata gagcctactt
     2401 tgctataaag ggtatggata ttttccttat agtttgtgcc aaacaagcgt accgttccat
     2461 ccgttgggag agcaagctgc aacatcattt tcattgctgt ggttttgcct gctccgtttc
     2521 tgccgagcaa accataaatt ttgcccgttg gcacatgaag attgacctta tcgacaacgg
     2581 tggctgttcc gtatcgcttc gtaagatttt ctgtttcaat gatataatcc atatttgatt
     2641 ttcctttccg ttggttgctg ttcttgtttt cactgaccac aattttatta tcacaggagc
     2701 tatatcaaaa gtcaagaaac ctaccgtcac atcgcctaca tctatcatgt caacatgaaa
     2761 aaaagccccg acacttatcg tgtcagagcc tatttcaagg gatttatatc gtttttactt
     2821 tgttccgttc tgcttaatcc aaacaactat ttttgcgaca aacagcagaa acataagtgc
     2881 tgttatatat ggttctcttg ctgctgctaa gaaacagata gccaaagcag tgagtccaag
     2941 ggagcatttt gtagcaatgc cgccccatgt ttctttatct aaatgggtaa gtattatttt
     3001 agcaatccct attacaatca aggcggcaaa agcactccag tagactgcaa gattgaatgg
     3061 tgtggtggct gtaaatgata gtagattgac cgcataaaca taaccgccaa cagagtttcc
     3121 atataacgga agaaatataa acgcaactgc catcatatcc aatattccat aaatatagtt
     3181 gtaaatcttt ttcaagttgg aacgattttc agtttcggca agtgtaatca gttcttcgcc
     3241 cgatagtagt tcatctatgg tcacagaaaa gaatttagaa atacacttga gcgactcaat
     3301 gttagggtaa cccttgccgc tttcccattt tgaaatggct gttcttgata catataattg
     3361 ctccgcaagt tgttcctgcg ttaagttctt tccagtccta agctgttgta gcttttcatt
     3421 aaattccatg attctcctcc tgtttgttcc ttgttttcat acgtccatca acgggttttt
     3481 ctgcatcttt tggtatcatc catgcacgac caaatttttc agttccgtca atgcgatttt
     3541 cctcacataa tttttgtatc cgtctttccg atatgcccca tttttcagaa gcggctttca
     3601 cagaaatgta atccataata tcaacttcct ttcgcaaaga gtatataatt attatatacg
     3661 gaatgacgaa taatagaaag tttctctcca ctaatacgaa attatacact actaaacata
     3721 aaatcatact tcgttcaaat atcatcaccc gaaatgtaca atgatatagg gtgatattaa
     3781 aatgtaccaa gatgaaagaa aacttgattt taagccgtta ggtatagcga taaaaaaagc
     3841 tcgggaagca aaagggtgga cacaggaata tcttgcccaa ctggtagacc ttacgccacg
     3901 ctctattatg ggttctgttg caaagtttcc gataagctca ttttgaggta aaataattga
     3961 aaaagatagc ttggaggaat gaagatgact cagttcaaag gaaaacaatt tcaaaaagac
     4021 gtgattatcg tagccgtcgg ctactatctt cggtacaacc tgagttatcg tgaagtgcag
     4081 gaaattctgt atgaccgagg aattcacgtt tgtcacacca caatttaccg ttgggtgcaa
     4141 gaatacggaa agattcttta tcaaatttgg aaaaagaaaa acaaacagtc cttttattca
     4201 tggaaaatgg acgagactta tatcaaaatt aaaggaaagt ggcattattt atatcgcgct
     4261 atcgatgtag atggcttaac cttagatatc tggttacgga aaaagcgcga cacacaggct
     4321 gcgtatgctt ttctaaaacg actgaagaac cagtttggag aaccaaaggt tctagtaacg
     4381 gataaagcac cctctattaa gagtgccttc agaaagcttc agaaaaatgg actgtatata
     4441 acaacagaac atcgaacaat caagtatctg aataatctga ttgaacaaga ccatcgtcca
     4501 attaagcgaa gaaacaaatt ttaccaaagt ttgcgaacag cctcaaccac gattaaaggc
     4561 atggaagcga ttcgaggaat ttataaaaaa agccgaaaag aagggtctct ttttggcttt
     4621 tccgtctgta cagaaatcaa aggactatta ggaatccctg cttaaataag aatcatcttg
     4681 aaaaactaat gacctttttg agactttgca acagaac
//
LOCUS       DAUCLR010000106         4714 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.107, whole genome shotgun sequence.
ACCESSION   DAUCLR010000106 DAUCLR010000000
VERSION     DAUCLR010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.107"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..319)
                     /locus_tag="VGT87_001657"
     CDS             complement(<1..319)
                     /locus_tag="VGT87_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase"
                     /protein_id="HEQ3814487.1"
                     /translation="MDLKITNGFYDPSHLSYEVVERKGLGHPDTLADGIAEQIEIDYS
                     LYCLDKFGVIPHHNFDKIIIRGGHSVQDFGGSDFIEPIKIIFLGRASKKCFNSSIPLF
                     KIQK"
     gene            complement(329..1315)
                     /locus_tag="VGT87_001658"
     CDS             complement(329..1315)
                     /locus_tag="VGT87_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HEQ3814488.1"
                     /translation="MSRNQDCKNILIIGFGKIGKIKAYKWLSRGYEVYVKDLKFSSMK
                     YLNTNSDVLDDLSRQYFTIEICTPTNHHLTLLKTVITRYVYTYISIEKPLCNRLEDLE
                     EMKSLVESHPHLAKKMFASEQYFFSKILEKLLQVDCFSLDKIKEITVNFSKDRIEDNE
                     NGRFLDKEILGYGIEIPHIIAVISYLGISLEEFKKGIFVNAIYIKDLEKHDYSIEILS
                     CINQTVIKIISNLGSFAIREGKIEGGDAKTVRRVTIDDKTILFDPHPKLERYKSELVT
                     GTNTFQIHDDMMDTYISLLEGNAIPEDCYINNAIEITKLLINLYSECEKIYL"
     gene            complement(1308..2042)
                     /locus_tag="VGT87_001659"
     CDS             complement(1308..2042)
                     /locus_tag="VGT87_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEQ3814489.1"
                     /translation="MNLSIFGLKNIPYLKEGDSIEKLIEESIKTSGFFIEDNDVLCIA
                     SKVVSVAEGQVMSLNEIQVSDVAKEIHRNIPRKDPRIIEIMLNLVNRDLSRLDIKKNY
                     IGCRLENGLKLTSGGIDRKSVDEVFLLPNNPDASAKRISEYLKKSLGKNVAVVITDSD
                     GREDKRGATQVAIGIYGIHPLRKTEVIDSQGETIKFQEETLCDMIAACAGLVMGQRGT
                     GIPAVLIRGLDYKWSENTSIKEALNE"
     gene            complement(2044..2880)
                     /locus_tag="VGT87_001660"
     CDS             complement(2044..2880)
                     /locus_tag="VGT87_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEQ3814490.1"
                     /translation="MTFHITVNLDEISDKLDDSLKFLNRLSIKSCELRMINTKNIIQL
                     SDAELINLKERLQKYNITPISIASPLFKWNIEENHLESNLDLYGISANVTLNEQWLYV
                     RKIIFIAKFLNIKYVRIFGGIGCSVDDFLNNELFLNLLKETEIIFLIENELGTSVSTA
                     DDLLKIGHFIDEKSIKNLKIWFDIANYYIVEPQVDKVIPKLKKYIYYIHCKNYVNNDL
                     GIHYVELGKGIIDYKTIIHEIHEHFIDLIFSLEVHEKEIDKKAEVVRKSYLTLNEYIN
                     GG"
     gene            complement(2877..3803)
                     /locus_tag="VGT87_001661"
     CDS             complement(2877..3803)
                     /locus_tag="VGT87_001661"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEQ3814491.1"
                     /translation="MLVLYVSSKLIKRRRMKFMYSALLGNPVEHSISDELFEYLNHNI
                     GVLDEYKHKKILVDSVCLEDKIKHLINDPKCIGLNITLPYKREVMRYIDNLDRVAEIT
                     GSVNNIYKSGNIITGTNTDWKGIYNTLVFFNISHITKCCVLGTGGTARAAIFTLQKLQ
                     ISPTVVYREPISENTRSLIKSFPDLKYITYKELADQQILNDIEIIINTTPVGMINYEE
                     SLPINFDHLDNVNYKDKYFMDVVFNPLHTKLHEYFGKRGAKIIDGLWVLIFQGIEAFS
                     LWSDKINGGKYFFSKDDVVLIHTFLEGKIKGK"
     gene            complement(3872..3943)
                     /gene="shp3"
                     /locus_tag="VGT87_001662"
     CDS             complement(3872..3943)
                     /gene="shp3"
                     /locus_tag="VGT87_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone SHP3"
                     /protein_id="HEQ3814492.1"
                     /translation="MKKISKFLPILILAMDIIIIVGG"
     gene            4023..>4714
                     /gene="rgg3"
                     /locus_tag="VGT87_001663"
     CDS             4023..>4714
                     /gene="rgg3"
                     /locus_tag="VGT87_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985634.1"
                     /note="Regulator gene of glucosyltransferase 3; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg3"
                     /protein_id="HEQ3814493.1"
                     /translation="MKSKLGATLRKVRKGKQISLCFVADEHLSKSQISRFERGESEIS
                     CIRLINILDKLHITLDEFLILHDEDYTNSETFANLVQYIRKQYSSQNINNIACLLSDT
                     SDYTLNSFEKTMVKSILHTMDSSIIPSDDELLQLTDYLFKVEKWGYYEILLLGNCVRT
                     INYNPYFLLTKEMLNNYIYSSRNKTNKRIVTQLAINCFILSINKEEFSNCSYLIDEIK
                     TLLDNELNFYEQT"
BASE COUNT         1556 a          860 c          569 g         1729 t
ORIGIN      
        1 ttttctgaat tttaaaaagt ggaattgaac tattaaaaca ttttttgctc gccctaccta
       61 gaaaaataat tttaattggt tcaataaagt cacttccacc aaaatcctga actgaatgtc
      121 cccctcggat aataatctta tcaaaattat gatgtggaat aacaccaaac ttgtccaaac
      181 aataaagtga gtaatcaatt tcaatttgct cagcaattcc atctgctaag gtatcagggt
      241 gacctaagcc tttgcgttct actacttcgt atgataaatg acttgggtca taaaagccgt
      301 ttgtaatttt taaatccata gttacacctc ataaataaat tttttcacat tctgaatata
      361 agttaattaa taattttgtt atctcaattg cattattgat atagcaatct tcaggaatag
      421 catttccttc taacaaactg atatacgtgt ccatcatatc atcgtgtatt tgaaacgtat
      481 ttgtcccagt caccaattca ctcttatacc tctcgagttt aggatgtgga tcaaatagta
      541 ttgttttgtc atcaatggta actcttctta ctgttttagc atcacctcct tcaatcttgc
      601 cctctcttat tgcaaaactt cccaaattac taataatttt aatcactgtt tgattaatac
      661 aagataatat ttctattgaa taatcgtgtt tctctaagtc ttttatataa atagcattca
      721 caaagattcc ttttttgaat tcttctaatg aaattcctaa ataagaaatg acagcaatga
      781 tatgaggaat ctctattcca tagcccaata tctctttatc taaaaatctc ccattctcgt
      841 tatcctcaat cctatcttta gaaaaattaa ctgttatttc cttgatttta tctagagaga
      901 aacaatctac ttgtaacagt ttttccaaaa ttttcgaaaa gaagtattgt tcactggcaa
      961 acattttttt tgctagatgt gggtgacttt ctactagcga cttcatttct tctaaatctt
     1021 ctaacctgtt acacagtggt ttttcaatgc taatataagt atagacatac cttgttataa
     1081 cggttttcaa tagcgtaagg tggtgatttg tcggagtaca aatctcaata gtaaaatatt
     1141 gtcgtgataa atcatccaac acatcactat ttgtattaag gtatttcatg ctggagaatt
     1201 tcaaatcttt gacatatacc tcatatccgc gtgatagcca cttgtacgct ttaattttcc
     1261 caatttttcc aaaaccaatg attagaatat tcttacagtc ctgatttcta ctcatttaac
     1321 gcttccttaa tactagtatt ttcagaccat ttataatcca agcccctaat gaggacagct
     1381 ggaattccag ttccacgttg ccccattact aagccagcac aagctgcaat catatcacac
     1441 aaagtctctt cttggaattt tattgtctct ccttgtgaat ctattacttc tgtttttctc
     1501 aaaggatgaa taccataaat accaattgct acttgagtag cacctctttt atcctctcta
     1561 ccatcactat ctgttataac aactgcaaca ttttttccaa gtgatttttt aagatattcc
     1621 gaaattcttt ttgcagaagc atccggatta ttaggtaaaa gaaatacttc atctactgat
     1681 tttctatcga ttccaccact tgtcaacttt aaaccatttt ccaagcgaca accaatgtaa
     1741 ttttttttga tatctaaacg tgacagatct ctattgacta aatttagcat aatttcaatt
     1801 attctaggat ctttacgagg aatattcctg tggatttctt ttgctacatc actcacttgt
     1861 atctcgttta aagacatcac ctggccttct gcgacagata caactttaga agctatgcaa
     1921 agcacatcat tatcttcgat aaaaaatcct gaagttttaa tgctttcttc aataagcttt
     1981 tctatactat ccccttcttt caaataagga atattcttta aaccaaatat actcaaattc
     2041 attttagcct ccattaatat actcatttag tgtcagataa gacttcctca ctacttctgc
     2101 ctttttgtct atttcttttt catgtacttc taaactaaaa attaagtcta taaaatgttc
     2161 atggatttca tgaatgattg ttttataatc gataattcct ttacctagtt ctacataatg
     2221 tattcctaaa tcattgttaa catagttctt acagtgaata tagtaaatat attttttcaa
     2281 ttttggaata accttgtcta cctgaggttc aactatgtaa taatttgcaa tatcaaacca
     2341 gatttttaga ttttttatag atttttcatc tataaaatgt cctattttta acaaatcgtc
     2401 tgcagtgctc actgaagttc ctaactcgtt ctcaattaaa aagataattt cagtctcttt
     2461 taacaaattc aaaaatagtt cattattcaa gaagtcatct actgagcacc caatcccacc
     2521 aaatattctt acatatttga tatttaaaaa ttttgcaata aaaataattt tcctcacata
     2581 aagccattgt tcatttaacg taacattagc tgaaataccg tataaatcaa gattactctc
     2641 taaatgattt tcttctatat tccatttaaa aagtgggctt gcaattgaaa ttggagtgat
     2701 attatacttt tgtaaccgtt cttttaaatt aattaactct gcatcactaa gttgtataat
     2761 atttttagtg tttatcattc gtaattcaca acttttaatt gataatctat ttaaaaattt
     2821 caatgaatca tctagtttgt ctgatatttc atcaagattt actgtaatat gaaaagtcat
     2881 tttcctttta tcttcccttc tagaaatgta tgaattagga caacatcatc ttttgaaaaa
     2941 aaatatttac caccattaat tttatcactc cacaatgaaa atgcttcaat tccctgaaaa
     3001 atcaaaaccc ataaaccatc aattattttt gctcctcttt taccaaaata ttcatgaagt
     3061 ttcgtatgta aaggattgaa aactacatcc ataaagtatt tatctttata attaacattg
     3121 tccaaatgat caaaatttat aggtaaactt tcttcataat ttatcatacc aactggtgta
     3181 gtatttatta tgatttctat atcatttaaa atttgttgat cagccaattc cttatatgta
     3241 atatacttta aatcagggaa actctttatc aaacttcttg tattctcact aattggctcc
     3301 ctgtatacaa ctgttggtga gatttgtagt ttttgtaaag tgaaaatagc agctctagca
     3361 gtaccgccag tgcctaaaac acaacatttt gttatatggc taatattaaa gaaaactaat
     3421 gtattataaa tacctttcca atcagtatta gttcctgtaa ttatatttcc agatttataa
     3481 atattgttaa cagatcctgt tatctctgct actctgtcta aattgtcaat atacctcatt
     3541 acctctcttt tataaggtaa agttatattt aatccaatac attttggatc atttatcaga
     3601 tgtttgattt tatcttctaa acacactgag tctaccaaaa tttttttatg tttatattca
     3661 tccagaactc ctatgttgtg atttagatat tcaaataatt catctgatat agaatgttca
     3721 actggattac caagcaatgc agaatacata aatttcatcc tcctacgttt aattagttta
     3781 ctactaacat aaagtacaag cattacacag agctaaccat tacctataag tctttagagt
     3841 atctaaagac ttaggtatac acctaagtaa cttacccacc aacaataatg ataatatcca
     3901 ttgctaaaat taaaatcggc aaaaattttg aaattttctt cataagtgga cttcctttca
     3961 gtttttattt attgtacaat aaaaaaaaac ggaattgatt tttgttggga aagtgggaaa
     4021 atatgaaatc aaaacttggt gccacactta gaaaagttcg aaaaggaaag caaattagtc
     4081 tgtgcttcgt ggcagatgaa cacctttcaa aatcacaaat ctctcgtttt gagcgcgggg
     4141 agtcagagat atcgtgtatc cgactgatca atattctaga caaattacac attactttgg
     4201 atgagttcct tattttacat gatgaagact ataccaattc cgaaacattt gctaacctgg
     4261 tacagtatat tcgtaagcaa tattcatcac aaaatatcaa taatatagca tgtttacttt
     4321 ccgacacttc tgattacact ctgaattctt ttgagaaaac aatggtaaag tccattttac
     4381 acacaatgga ttcaagcatc attccatcag atgatgaatt acttcaacta acagattatc
     4441 tctttaaagt tgaaaaatgg ggctactatg aaatcctttt attgggtaac tgtgtacgaa
     4501 caattaacta caacccctac ttcctattga caaaggagat gttaaataat tacatttact
     4561 cttcacggaa caaaactaat aaacgaattg tcactcagct tgctataaat tgtttcattc
     4621 ttagcatcaa taaggaggaa ttttctaatt gctcctattt aattgatgag ataaaaacct
     4681 tgttagataa tgaattaaac ttctatgaac agac
//
LOCUS       DAUCLR010000107         4577 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.108, whole genome shotgun sequence.
ACCESSION   DAUCLR010000107 DAUCLR010000000
VERSION     DAUCLR010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.108"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..830
                     /locus_tag="VGT87_001664"
     CDS             <1..830
                     /locus_tag="VGT87_001664"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEQ3814494.1"
                     /translation="YVEFETLTSDFTTKTNWFSPEAIEDLPEYLDVPKANDTATMISY
                     WPLTISEELALMIEGYFYKLDKNELPTPRFTKMGGDIKVMVVRNDLEYSIRINFPLIS
                     KFFNNDIESQLYVDKHVEKIKKYIEQKYKNISFSIDYHYYLTTTGSCIDFGEEGAVGR
                     GNKTHGIISSFRPNTMEAPAGKNCTYFVGKVWGFLSDTIAKEIYEAFNTPCQIIMQLN
                     IGSKLYRPTHLFIQTEESVDQERVLEIVNRHLNNGKQNTNLILSTQHFIPKTNVYDG"
     gene            814..1782
                     /locus_tag="VGT87_001665"
     CDS             814..1782
                     /locus_tag="VGT87_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEQ3814495.1"
                     /translation="MFMMDKTVLYFGLVEISFDVPDFIFQKIKEQNNKIRYYSKSINI
                     LGSVKFINSNQQLIEYDGNGNILVYGRLKYFKEGISLYYIGEYLAECLLIEHSDTLLI
                     HAAAVYNPLSGHSILLLGDKGAGKTTVAIRLCIEHGYHLIGNDQVIFGSNSGILLTYA
                     GTSFFKIRRTAVLSDNLLFKLFSKFFNRSLQFNKASWDDKISIFPKELGIRTCNFSTE
                     ISKIYYIKTDKKEKQIYHSIWSDSLASLYLNELLGRHISGQVACFQSDNGKYFSTMPL
                     INYEKNNRVREQIVKGIFAKQKLFKITAATPAKIAEAIIENVKKNS"
     gene            1837..2826
                     /locus_tag="VGT87_001666"
     CDS             1837..2826
                     /locus_tag="VGT87_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEQ3814496.1"
                     /translation="MFVIGIPTLNEADNISRLVKQIDEYAVNLGKEIIIINSDSKSTD
                     GTPQIFLETKTYNTKVSIVSEAKGKGYNVRNIFEYAINHVPNFSGLILIDGDVVSMKK
                     MWLEKMFIAIESGNDLIIPNYARKSFEGNATNHFIYPMLVKFFKRDMPYQCISGDFGF
                     SRGLIKDLTLKCNWHKYTLGYGIDIFLTLTAILKSYKIKEIDLQSKIHKKSFEKIEKI
                     FLEVSQSFFETINDNSLNQDKLRLNINFESHSRQFIKSSDILSSNDIENLKLRALFLL
                     QEEKQYPHGLSEVEWDGILSNTINNIYRYSSEEHSLYLLPLYLLRVYNYLKYN"
     gene            3518..>4577
                     /locus_tag="VGT87_001667"
     CDS             3518..>4577
                     /locus_tag="VGT87_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEQ3814497.1"
                     /translation="MKITVVGIGYVGLSIGLLLAKEHDVTFFDIDNKKIDLINKRQSS
                     LKEAAINKLLCKTKNINATSSEELAYKDATFIILSLPTNLKVNKLDTSIIEISVSNIL
                     KINKKATIVIKSTVPIGFTEYLRNRFHYNDIIFSPEFLREGSTIHDQLYPSRTIVGNE
                     SRNSQLFLDILTDISVEKDSPSLLVGSSEAEAIKLFSNAYLAQKIAFFNELDTFAEMQ
                     NLDSKKIIEAIGYDQRIGNSHNNPSFGFGGYCLPKDIKQLEYHFKEIPAPIITSISES
                     NLLRKIHIAKMILNSSAKTIGIYRINSKKDSDNCRESSTIDVAKLLKSSGKDVIIFEP
                     LINQKKFLGCPLINDFNEF"
BASE COUNT         1697 a          550 c          780 g         1550 t
ORIGIN      
        1 attatgtaga atttgagaca ttgacctcag attttactac aaaaactaat tggttctcgc
       61 ctgaagcaat agaagattta ccggagtatc tagatgtacc aaaggcaaat gatacagcta
      121 caatgataag ttattggcca ttaacaattt ctgaagaatt agcattaatg attgaagggt
      181 atttttataa attagataag aatgaattac caacgccaag atttactaaa atgggtggtg
      241 atattaaagt aatggtcgta aggaacgatt tagaatattc tattagaata aattttcctt
      301 taataagtaa attttttaac aatgatattg aaagtcagtt gtatgttgat aaacacgtag
      361 aaaaaattaa gaaatatatt gaacaaaagt ataaaaacat aagcttttct attgattacc
      421 attattattt aactactaca ggttcttgta ttgatttcgg agaagaaggt gcagttggtc
      481 gaggtaataa aactcatggt atcatatcaa gttttaggcc aaatactatg gaagcaccag
      541 ctggaaaaaa ttgtacttat tttgtaggaa aggtttgggg atttttgtct gatactattg
      601 ccaaagaaat ttatgaagca ttcaatactc cctgtcaaat cattatgcaa ttaaatattg
      661 gatcaaaatt gtatagacca acccatttat ttattcagac agaagaaagt gttgatcaag
      721 aaagagtatt agaaattgtt aacaggcatt taaataatgg taaacaaaat actaatttaa
      781 tcttgtcaac tcaacatttt attcctaaga ccaatgttta tgatggataa aactgtttta
      841 tattttgggt tggttgaaat ttcttttgat gtgcctgact ttatatttca aaaaataaaa
      901 gagcagaata ataaaattag atattatagc aaatcaataa atatattagg tagtgtaaaa
      961 tttattaaca gcaatcagca acttatagaa tatgatggca atggaaatat tttagtttat
     1021 ggaagattaa agtattttaa agaaggaatt tcattatact acattggaga atacttagct
     1081 gaatgtctct tgattgaaca tagtgacacc ttattaattc acgcagcagc agtttataat
     1141 cctttatctg gccattcaat tttactatta ggagataaag gtgctggaaa aactacggtt
     1201 gcaataaggc tttgtataga acatgggtat caccttattg gtaatgacca agtaatattt
     1261 ggttcgaact ctggaatact actaacttat gcagggacat ctttctttaa aataagaagg
     1321 acagctgttt tgtcggacaa tttactgttt aaattattta gcaaattttt taaccgttca
     1381 ttacaattta ataaggcaag ttgggatgat aaaatttcaa ttttcccaaa ggagttggga
     1441 atacgaactt gtaatttttc tactgaaatc tcaaaaattt attatataaa gacagacaag
     1501 aaagaaaagc aaatttatca ttcaatttgg agtgatagtt tagcaagtct atatctaaat
     1561 gagctattgg gaaggcatat ttctggacag gttgcatgtt ttcaatcaga taatggaaaa
     1621 tatttttcta caatgccttt gataaattat gaaaaaaata atcgggtacg agaacagata
     1681 gtgaagggta ttttcgctaa acaaaaatta tttaaaatta cagcagctac gccagctaag
     1741 attgcagaag cgattataga aaatgttaag aaaaactcat agtgtgtgtg aatttgggga
     1801 ttaatatgaa gtattgaaaa aggagtaaga gtaattatgt ttgtgatagg cattccaact
     1861 ttaaatgagg cagataatat cagtagatta gttaaacaaa ttgatgagta tgctgtaaat
     1921 ttagggaaag aaattattat tattaattcg gatagtaaaa gtacagatgg aactccgcag
     1981 atttttttag aaaccaaaac ttataacacc aaagtttcga tagtatcaga agctaaagga
     2041 aaaggttata atgtgagaaa tattttcgaa tatgcaataa atcatgtgcc gaatttctcc
     2101 ggtttaattt tgattgatgg agacgttgtc tcaatgaaaa aaatgtggtt agaaaagatg
     2161 tttatagcaa ttgaatctgg aaatgatctt ataatcccaa attatgctag aaaatctttt
     2221 gaaggaaatg cgacaaacca ttttatttat cccatgttag tgaaattttt caaacgtgat
     2281 atgccttatc aatgcatctc tggtgatttt ggattttcaa gaggattgat aaaagattta
     2341 actttgaagt gtaattggca caaatataca ttaggttacg ggatagatat ttttttaacc
     2401 ttaacagcta tattaaagtc atacaaaatt aaggaaatcg atttacaatc aaagattcat
     2461 aaaaaaagct ttgagaagat tgaaaaaatt tttttagaag tttcacaaag tttcttcgaa
     2521 actattaatg ataattcttt gaatcaagat aagttacgtt taaatataaa ttttgaaagt
     2581 cattcaagac agtttattaa gtccagtgat atcctatcca gcaatgatat cgagaattta
     2641 aagttaagag cgttgttttt attacaggaa gaaaaacagt atccacatgg gctgtcagag
     2701 gtagaatggg acggaatttt gtctaataca attaacaata tttatagata tagtagtgag
     2761 gaacattcgt tatatttatt accactttat ttattgagag tatacaatta tctaaagtac
     2821 aactaagttt tcaattttta ttaatcttga gaagaagtgt gattggtgga atatatttca
     2881 atatgtttag cttatctaaa taataaatac agtatgtaag taaatttatt tataatattt
     2941 ttgatacttt gagtagtaac gataatattt aattttttga tggattactt taattgactt
     3001 tagtgtgtta gatgccagtt tggatttttg atatttgtgt tgagtttaaa agataaaaac
     3061 acttaatgaa aatctgtttt ttcttagaaa ggggaccagt atgattgaga cgctaatgag
     3121 tacgttggaa atggttagaa aaaaatccta gtaggactgc aatcgaagat gactttgagg
     3181 aatataaaaa gagaaatcta ccaatctttg tcagtgaaga tgatagtgtg atctattata
     3241 gaccagggtt catttttaat gaattctatc ccaatatttc aggttggaga caagaactaa
     3301 agactaagct tactagtatt tctttaattt ctttacattt tattggtgtt gttgacgaac
     3361 aaaaaaaggg ggaatatcta ggttcttttt ctgatacatc tgaattctta agtagacatc
     3421 caaaaattta tatcatctca tttaggtata aaaatcaatt ttatattctt tctaacaaaa
     3481 aaattgattt ataaaaagtt ttagaataga ggttaacatg aaaattacag ttgtaggcat
     3541 tggatacgtt ggattatcga taggactcct acttgcaaag gaacacgacg ttaccttttt
     3601 tgatattgat aataaaaaaa ttgatttaat aaataaaagg caatcctctc ttaaagaagc
     3661 agctataaac aaacttttat gtaagacaaa aaatattaat gcaacttctt ctgaagaatt
     3721 agcatataag gatgcgactt tcataatctt gtctttgcca accaacctaa aagttaataa
     3781 gcttgatact tccattatcg aaatttctgt aagtaatatt ttaaagataa acaaaaaggc
     3841 tacaattgta ataaagtcga cagttccaat tggttttaca gaatatttaa ggaatcgatt
     3901 tcactacaac gatatcattt tttcacctga gttccttagg gaaggatcaa ctattcatga
     3961 tcaattgtat ccttcgagaa ctatagttgg aaatgaatct agaaattctc aattattctt
     4021 agacatacta acagatatat cggttgaaaa agactcgcca tctttattag ttggctcttc
     4081 tgaagcagaa gcgataaagt tattttcgaa tgcatacttg gcacaaaaaa ttgctttttt
     4141 taatgagttg gatacgtttg ctgaaatgca aaatttggac tcaaaaaaaa ttattgaggc
     4201 tataggatat gaccagagaa taggaaattc gcacaataat ccttctttcg gttttggtgg
     4261 gtactgtctt cctaaggata ttaagcaatt agagtatcat tttaaagaaa ttccagcacc
     4321 aattattacc agtataagtg agtctaattt attaagaaaa attcatatag caaaaatgat
     4381 tttgaacagc tcagctaaaa caataggaat ttatagaatt aattccaaaa aagattcgga
     4441 taattgtagg gaatcttcta caattgatgt tgctaaactt ttaaaaagca gtggtaagga
     4501 tgttatcatc tttgagccat taattaacca aaaaaagttt ttggggtgcc ctttaattaa
     4561 tgattttaat gaattta
//
LOCUS       DAUCLR010000108         4520 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.109, whole genome shotgun sequence.
ACCESSION   DAUCLR010000108 DAUCLR010000000
VERSION     DAUCLR010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.109"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..136
                     /locus_tag="VGT87_001668"
     CDS             <1..136
                     /locus_tag="VGT87_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEQ3814498.1"
                     /translation="LRDLENGQCLISDLYGRVGVIQFHPVFEELLHAFDTRPPVRKEV
                     "
     gene            139..2316
                     /locus_tag="VGT87_001669"
     CDS             139..2316
                     /locus_tag="VGT87_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEQ3814499.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQNPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            2313..3314
                     /locus_tag="VGT87_001670"
     CDS             2313..3314
                     /locus_tag="VGT87_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEQ3814500.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            3311..4246
                     /locus_tag="VGT87_001671"
     CDS             3311..4246
                     /locus_tag="VGT87_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEQ3814501.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYYKVNPHKKVVIALWV
                     LLGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKS
                     IDNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQ
                     LITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSI
                     TTNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVT
                     VSLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
BASE COUNT         1436 a          874 c          951 g         1259 t
ORIGIN      
        1 attgcgtgat ttggaaaacg ggcaatgcct tatcagtgat ttatatggtc gtgtcggtgt
       61 gatacagttc caccctgtat ttgaagaact gctccatgcc tttgatacca gaccacctgt
      121 gcgaaaagag gtgtaaatgt gaaaccatca atagtaaaca gaataaaatc aaactggacg
      181 ctgaaacgtc taggtaaagt ggcaatgaca gtggctttca cacttgtgat tgccattttt
      241 cttttagcca tgctgggaac ggtggttcaa gctgcgggct tggtagatga tacggtcaat
      301 gtggcaaatg aatacagccg atacccactt gaaaactatc aactggattt ttatgtggat
      361 aatagctggg gctggcttcc gtggaactgg tcggacggga ttggaaaaca ggtcatgtat
      421 ggactatatg ccattaccaa ttttatttgg acaatcagtt tgtatgtttc caatgcgaca
      481 ggttacttag tacaggaagc ctattcctta gacttcattt ccgctacagc agattccatt
      541 ggtaagaata tgcagacctt agctggtgtg agtgcaaacg gattttcaac agagggtttc
      601 tatgttggat tcctcttact cttgattttg gttcttgggg tttatgttgc ctatacggga
      661 ctgataaaga gagaaaccac aaaggcaatt catgccatta tgaattttgt gctggtgttt
      721 atcctatcgg cttcctttat tgcctacgct cccgactaca ttaaaaaaat caatgacttt
      781 tcatcagaca tcagtaatgc cagtttatca cttggcacga agattgtcat gccccattcc
      841 gatagtcaag gcaaggacag cgtggactta atcagagata gcctgttttc catacaggtt
      901 cagcaaccgt ggctactgct tcaatacaac agttcagaca ttgaaagtat cggtattgac
      961 cgtgtggaaa gcctgctctc caccagccca gattccaaca atggcgaaga cagagaaaaa
     1021 attgttgcgg aagaaattga agacagaagc aataccaatc taaccattac aaagaccatt
     1081 aaccgtttag gtacagtctt cttcctattt gtcttcaata ttgggatttc catatttgta
     1141 ttcctattaa caggaatcat gattttctcg caggtacttt ttatcatcta tgctatgttt
     1201 ctgcctgtga gctttatttt aagcatgatt ccatcatttg atggtatgtc aaaacgagcc
     1261 ataacaaagc tctttaatac cattttgaca cgagctggaa tcacattgat tattacgaca
     1321 gcatttagta tttcaaccat gctctatacc ttatcggctg gttatccgtt ctttttgatt
     1381 gcttttctac agattgtgac ctttgcagga atctacttca agctgggcga tttaatgagt
     1441 atgttttctc tacagagtaa cgattctcaa agtgtgggaa gtcgtgtgat gagaaaacct
     1501 cgtatgctta tgcacgctca catgcaccgt ctacagcgga aacttggacg ttccatgact
     1561 actctagggg ctgggtctgc cattgttaca ggtaaaaaag gacagtcggg ttcggggagt
     1621 tctgcaagga cacaagcaga tcactcccga ccagacggaa aggaaaaatc aacacttgga
     1681 aaacgtatcg gtcaaaccat cggtacagta gctgatacca aagacagaat ggtagacact
     1741 gctagtggtt tgaaagaaca ggttaaagat ttgccgacca atgcaagata tgcagtatat
     1801 caaggaaaat ccaaagtaaa agagaatgtc cgtgatttaa ccagtagtat ttctcaaacc
     1861 aaagcggaca gagccagtgg acgcaaggaa cagcaggaac aaaggcgaaa aaccattgcg
     1921 aagcgtcgct ctgaaatgga acaggtcaaa cagaaaaaac agcctgcttc ttctgttcat
     1981 gaaagaccga ctacaagaca agaacaatat catgatgaac agacctcaaa acagtctaat
     2041 attcagactt catataagga atctcaacaa gccaaacaag agcgtccagc agttaagtcc
     2101 gatttttcaa gtccaaaagt ggaacgccaa ggcaataccg ttcaagaaaa aaccgttcaa
     2161 aatccagcaa cttcaaccac tacagcagat agaacttcac aacgtccaat cacaaaagaa
     2221 cgtccgtcta ctgttcaaag agtaccacta caaaatacaa gaagtagacc accaatcaaa
     2281 accgccacca ttaagaaagt cggtaagaaa ccatgaagtt gaaaacttta gtgattggtg
     2341 gttctggatt attcttgatg gtcttctcac tgcttctgtt tgttgccatt ttattttcag
     2401 atgaacagga cagcggaatt tccaatattc attatggagg tgtgaatgtt tccgcagaag
     2461 tgctggctca taagcctatg gtagaaaaat atgccaaaga atatggcgtt gaagaatatg
     2521 tcaacatact tcttgcgatt atacaggtgg aatcgggcgg tactgcggaa gatgttatgc
     2581 agtcctcgga atccctcggt cttccaccta attcattgag tacagaagaa tccattaagc
     2641 aaggtgtgaa gtatttcagt gaattattag ccagtagcga aaggctcagt gtagatttag
     2701 aatcggttat ccagtcctac aattatggtg gtggtttctt agggtatgtg gctaatcgtg
     2761 gaaataaata tacctttgaa ctggctcaaa gtttctcaaa agagtattca ggtggcgaaa
     2821 aagtgtctta ccccaatccc atagccatac ctatcaatgg gggctggcga tacaactatg
     2881 gcaatatgtt ttatgtgcaa ctggtaacgc agtatcttgt cacaacagag tttgatgatg
     2941 atacggtaca agccatcatg gacgaagcac tgaaatatga gggctggcga tacgtttacg
     3001 gtggagcttc cccgactact tcttttgatt gtagcggact gacacaatgg acgtatggaa
     3061 aagctggaat taacttacca cgaaccgcac aacagcaata tgatgtgacc cagcatatcc
     3121 cactatcgga agcacaagct ggcgatttgg ttttctttca ttctacctat aacgctggct
     3181 cttatattac tcatgttggg atataccttg gcaataaccg tatgtttcat gcaggcgacc
     3241 caatcggtta tgccgactta acaagcccct actggcaaca gcatttagtg ggagcaggac
     3301 gaatcaaaca atgagaaagg aagatttaat gatgaaattt agaaaaaatc agaataaaga
     3361 aaaacagata ccaaaggaaa agaaacctcg tgtctactat aaggtcaatc ctcataaaaa
     3421 ggttgtgatt gccttgtggg tacttttagg gcttagtttc agctttgcga tattcaagca
     3481 ctttacagct atagatactc atactattca cgaaacaact atcatagaaa aggaatacgt
     3541 tgatactcat catgtagaaa attttgtaga gaactttgcg aaagtctact attcatggga
     3601 gcaatccgat aagtccattg ataatcgaat ggaaagtcta aaaggctatc tgacagatga
     3661 acttcaagct ctcaatgttg atacagtacg caaagatatt cctgtatcgt cttctgtaag
     3721 aggatttcag atatggacgg tagagccaac tggcgacaat gagtttaatg taacctacag
     3781 tgtagaccag ctcattacag agggagaaaa tacaaagacc gtccactctg cttatatagt
     3841 gagtgtctat gtagatggtt ctggaaatat ggtactggtt aagaatccga ccattaccaa
     3901 catacctaag aaatcaagtt ataaaccaaa agccattgaa agtgagggga cggttgattc
     3961 cattacaacc aatgaaatca atgagttttt aacgacgttc ttcaagctct atcctacagc
     4021 gacagccagt gaactttcct actatgtgaa tgacgggata ttaaaaccaa tcggaaaaga
     4081 gtacatcttt caagaactgg taaatcctat tcacaatcgt aaggataatc aagtcacggt
     4141 atcgctgaca gtggagtata tcgaccagca gaccaaagca acgcaggtat ctcaatttga
     4201 tttggtactt gaaaagaacg ggagtaattg gaagattata gaataacaaa tattggtaca
     4261 ttattacagc tattttgtaa tcacgtactc tctttgataa aaaattggag attcctttac
     4321 aaatatgctc ttacgtgcta ttatttaagt atctatttaa aaggagttaa taaatatgtg
     4381 gcaaggtatt cttaaataaa ctgtcaattt gatagtggga acaaataatt ggatgtcctt
     4441 ttttaggagg gcttagtttt ttgtacccag tttaagaata cctttatcat gtgattctaa
     4501 agtatccaga gaatatctgt
//
LOCUS       DAUCLR010000109         4277 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.110, whole genome shotgun sequence.
ACCESSION   DAUCLR010000109 DAUCLR010000000
VERSION     DAUCLR010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.110"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(447..629)
                     /locus_tag="VGT87_001672"
     CDS             complement(447..629)
                     /locus_tag="VGT87_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HEQ3814502.1"
                     /translation="MLTYDEFKQAIDNGYITADTVMIVRKNGQIFDYVLPNEKIKNGE
                     IVTDEKVEEVLVELSR"
     gene            869..1627
                     /gene="mf2"
                     /locus_tag="VGT87_001673"
     CDS             869..1627
                     /gene="mf2"
                     /locus_tag="VGT87_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNase Mf2"
                     /protein_id="HEQ3814503.1"
                     /translation="MKLSKQKASLLTAVLLLLSLSITTITVDAAKVRTYPNVSHANTH
                     YKNTASSKLLPFTANYQLQLGELDNLNRATFSHIQLQDRHETKDVRTKINYDPVGWHN
                     YQFPYGDGSKSSWVMNRGHLVGYQFCGLNDEPRNLVTMTAWLNTGAYSGANDSNPEGM
                     LYYENRLDSWLALHPDFWLDYKVTPIYSGNEVVPRQIELQYVGIDSSGELLTIRLNSN
                     KESIDENGVTTVILENSAPNINLDYLNGTATPKN"
     gene            1738..2445
                     /gene="speC"
                     /locus_tag="VGT87_001674"
     CDS             1738..2445
                     /gene="speC"
                     /locus_tag="VGT87_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021733756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptococcal pyrogenic exotoxin SpeC"
                     /protein_id="HEQ3814504.1"
                     /translation="MKKINIIKIVFIITVILISTISPIIKSDSKKDISNVKSDLLYAY
                     TITPYDYKDCRVNFSTTHTLNIDTQKYRGKDYYISSEMSYEASQKFKRDDHVDVFGLF
                     YILNSHTGEYIYGGITPAQNNKVNHKLLGNLFISGESQQNLNNKIILEKDIVTFQEID
                     FKIRKYLMDNYKIYDATSPYVSGRIEIGTKDGKHEQIDLFDSPNEGTRSDIFAKYKDN
                     RIINMKNFSHFDIYLEK"
     gene            complement(2514..3266)
                     /locus_tag="VGT87_001675"
     CDS             complement(2514..3266)
                     /locus_tag="VGT87_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEQ3814505.1"
                     /translation="MATLDEVLSFAKGLADAGQGVDLDNVYGTQCVDLPNWITTKYFS
                     IALWGNAIDLLDSAAAQGMEVVYNAPGVNPRAGAIFVMVTYAHGYGHTGLVIVTSDGY
                     VLHNIEQNVDGNADALYIGGPARYVDRPFEDGTGYILGWFYPPYDSTPAAVTEPSAPV
                     VAQSDGTYVANPETGTFTVRVAALNVRSAPRLDAEIVATYGENMEFNYDGWIDSDGYI
                     WVTYISVTGVRRYVSVGNSENGRRVTNFGTFR"
     gene            complement(3384..3839)
                     /locus_tag="VGT87_001676"
     CDS             complement(3384..3839)
                     /locus_tag="VGT87_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEQ3814506.1"
                     /translation="MIIDLTSLIHLFGDLVRTVEIHVFTLFVCFDIITGLTKGITNKR
                     ANSTKGLSGIIKHFLVVLLVYTVYPYLILLGAKPLAVAFVLFFIACYGISIVENWGQL
                     GLPMPSFVRSFFEKLKRDTDQFDIATIKIDKTGVKLEAPQVDLKQKEEE"
     gene            complement(3849..>4277)
                     /locus_tag="VGT87_001677"
     CDS             complement(3849..>4277)
                     /locus_tag="VGT87_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814507.1"
                     /translation="EYVEIAMSEAVQKVDDLEKISQETAKTAKTAQTAAGLAKVSAER
                     TQRMINLQTIHVLTSGGKVEPDIYKGMLELIEPAKKGEYQAYDVFTVVDDKHEEQAGE
                     GNLVFVHVNDSFTYETQTLEDLESEDKVTVIKYADLVKQD"
BASE COUNT         1428 a          840 c          681 g         1328 t
ORIGIN      
        1 agaaacattc taaataaact tttttaaact taccttttgc tttcaaaaat cctaacttca
       61 actatttgca aatatgaaaa aaactccccc tacctgcgga aatgatatac taatagaagg
      121 atgactgacc attatagagt gcatgcatta gaacttagct taactgaaga gaaaactact
      181 aactttatgc cccaaatata aaaaaagaac cttgtcgcta tcaataaaca tcgatagcaa
      241 caagattttt ctagacgaat tatttaacag cgtcttttaa agattactta atattgtgca
      301 catataatat aataaacttt gattttataa agctacaaaa taacgaaact ttatataatt
      361 gcttcgaatc aactatgtaa gttttaaact tatgccccct atttacccct ttaataaaac
      421 tacttctcag ggggcaaaga aaatacttat ctcgaaagct ccaccagcac ctcttccact
      481 ttttcgtctg tcacaatttc tccattcttt atcttctcat tcggcaacac ataatcaaaa
      541 atctgtccgt tcttgcgcac gatcattact gtgtctgctg tgatatatcc attgtcaatt
      601 gcttgtttaa attcgtcgta tgttagcata aagcacctcc ttatcttatt attcgtattt
      661 tacgagaata tagtacactt aaatttaaaa cttattaaaa tttaataact tctattttat
      721 taaacaaaaa gaaattttag cgtaatatta ttgataaatt aattttatta attttaagtt
      781 tttttaattt aaagtgatat aattaactag aaaagtgcaa tatttgatcc actctaaaag
      841 ctaaaaatgg aaaggtttag gtgttatcat gaaattatct aaacaaaagg caagtttgct
      901 taccgctgtt ttactgttac tttctctttc tataacgaca ataactgttg acgcagctaa
      961 ggtacgaact tatccgaatg tatcccatgc aaacacacat tataaaaata ctgcttctag
     1021 taagctctta ccttttactg ctaattatca gttacaactt ggtgagttgg ataaccttaa
     1081 ccgcgctacc ttttcacata tccaactgca agatagacac gaaactaaag atgtacgcac
     1141 taaaataaat tatgaccctg tgggatggca taactatcaa tttccatacg gagatggctc
     1201 aaaatcttca tgggtaatga atcgtgggca tctagtcgga tatcaatttt gtggattaaa
     1261 cgatgaacca agaaatttag tgacaatgac agcctggcta aacacaggtg cttattcagg
     1321 agcaaatgac agcaatcctg aagggatgtt atactatgaa aaccgtttag attcttggct
     1381 tgcacttcac cctgactttt ggctagatta taaagtcact cctatatata gtggcaatga
     1441 agtagtgcct cgacaaattg aattacagta tgttggaatt gattcatctg gtgagttgct
     1501 tactataagg ctaaatagta ataaagaaag tattgatgaa aatggtgtta ctacagtaat
     1561 cttagaaaac tctgctccaa atatcaacct tgactattta aatggaactg ccactcctaa
     1621 aaactaaaat ataaatacat ttataaaatt tctaaataaa cagaaatctg atttttaact
     1681 acttactgct atttcatgta ttctcgtacg agtaatacat ttaattaagg agaaaaaatg
     1741 aaaaagatta acatcatcaa aatagttttc ataattacag tcatactgat ttctactatt
     1801 tcacctatca tcaaaagtga ctctaagaaa gacatttcga atgttaaaag tgatttactt
     1861 tatgcataca ctataactcc ttatgattat aaagattgca gggtaaattt ttcaacgaca
     1921 cacacattaa acattgatac tcaaaaatat agagggaaag actattatat tagttccgaa
     1981 atgtcttatg aggcctctca aaaatttaaa cgagatgatc atgtagatgt ttttggatta
     2041 ttttatattc ttaattctca caccggtgag tacatctatg gaggaattac gcctgctcaa
     2101 aataataaag taaatcataa attattggga aatctattta tttcgggaga atctcaacag
     2161 aacttaaata acaaaattat tctagaaaaa gatatcgtaa ctttccagga aattgacttt
     2221 aaaatcagaa aataccttat ggataattat aaaatttatg acgctacttc tccttatgta
     2281 agcggcagaa tcgaaattgg cacaaaagat gggaaacatg agcaaataga cttatttgac
     2341 tcaccaaatg aagggactag atcagatatt tttgcaaaat ataaagataa tagaattatc
     2401 aatatgaaga actttagtca tttcgatatt tatcttgaaa aataattcat catacacaaa
     2461 aaaccgccca gaaattatct gagcggtttt gtcttatctc ggagctttac ctcctatcta
     2521 aaagtaccaa agttggtcac acgtcgtccg ttttcggaat ttccgacgga cacatatcgt
     2581 ctaacaccag taacactgat gtatgtcacc caaatataac catctgagtc aatccaacca
     2641 tcatagttaa attccatgtt ttcgccataa gttgccacaa tttctgcgtc taatcgaggc
     2701 gcagaacgga cgtttaaagc agcaacacga acagtaaaag tacctgtttc agggttagct
     2761 acataagtac catctgactg tgcaaccact ggagcgcttg gctctgtcac tgctgctggc
     2821 gtactatcgt aaggagggta aaaccaaccc aaaatatatc cagtgccatc ttcaaatggg
     2881 cggtcaacat atcgagctgg accaccgatg taaagggcgt cagcattacc atccacgttt
     2941 tgctcgatgt tatgcagaac atatccgtct gacgtcacaa taactaatcc agtatgaccg
     3001 taaccatgag cataggtcac catcacaaag atagccccag ctcgtggatt aactccagga
     3061 gcattataga ccacttccat tccttgggca gctgcactat ctagtaagtc aatagcattg
     3121 ccccaaaggg caatgctaaa atattttgtc gtgatccagt ttggcaagtc cacgcactgc
     3181 gtaccgtaaa cattatcaag atcaactcct tggccagcat ccgctaatcc ttttgcaaaa
     3241 gacaagactt cgtctaaagt tgccatatca gttacttcct ttcttcaaaa aatgggctta
     3301 aaatcaaagc aatcacagat aatggcacat acagtattgc gattgctata actaatgcta
     3361 atcgtgtgac tgcttgtctc atgctactcc tcttcttttt gttttaaatc aacttgtggc
     3421 gcctcgagtt taacacctgt tttatcaatt ttgattgtcg caatatcaaa ctggtctgta
     3481 tcacgtttga gcttttcgaa aaatgatctg acaaaacttg gcattggcaa acctagttgt
     3541 ccccaatttt ctacgattga aataccataa caggcgataa aaaataggac aaaggcgact
     3601 gctaaaggct tagctccgag caagattagg taaggataga cagtatagac taacaacaca
     3661 actaaaaagt gtttaataat gccagatagc ccttttgtac tgtttgctct cttattagta
     3721 ataccttttg tcagtcctgt aataatgtca aaacagacaa aaagcgtaaa aacgtggatt
     3781 tcgacggtac gaactaaatc cccgaaaagg tgaataagac ttgttaaatc aataatcatc
     3841 taaccacctc aatcctgctt aactaagtcc gcatacttaa tgactgtgac tttatcctct
     3901 gattctaagt cctctaaggt ctgtgtctca taagtaaacg agtcgttgac atggacaaag
     3961 actaagttgc cttcgccagc ttgctcttcg tgtttatcgt cgaccaccgt aaagacatca
     4021 taggcttgat actcaccttt tttggctggc tcaataagct ctaacatacc tttgtagata
     4081 tcgggttcaa ctttcccacc acttgtcaat acgtggatgg tttgcaagtt aatcatccgc
     4141 tgtgtacgct ctgcggacac cttagctagt ccagcggctg tttgggctgt tttagcagtc
     4201 ttagcggttt cttgtgagat tttttccaag tcatcaactt tttggactgc ttcgctcata
     4261 gcgatttcga catattc
//
LOCUS       DAUCLR010000110         4186 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.111, whole genome shotgun sequence.
ACCESSION   DAUCLR010000110 DAUCLR010000000
VERSION     DAUCLR010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.111"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..668
                     /locus_tag="VGT87_001678"
     CDS             <1..668
                     /locus_tag="VGT87_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEQ3814508.1"
                     /translation="AIKENQNALRTQQADLEVAKVNLAIQLASAEDEKASLLNQQAAA
                     QQAAAEALAAQQAAQAKAREEAAQQAASVEAAKSAITPAPQATPAAQSSNAIEPAALT
                     APASPSARPQVSYSSVNNYPVGQCTWGAKSLAPWAGNNWGNGGQWAASAQAAGYSVGS
                     TPMVGAIAVWNDGGYGHVAVVVEVQSSSNIRVMESNYSGKQYIADHRGWFNPTGVTFI
                     YPH"
     gene            921..1883
                     /locus_tag="VGT87_001679"
     CDS             921..1883
                     /locus_tag="VGT87_001679"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEQ3814509.1"
                     /translation="MSYSDLKLFALSSNKELAEKVASAMGLQLGKSTVRQFSDGEIQV
                     NIEESIRGHHVFILQSTSSPVNDNLMEILIMVDALKRASAEKISVVMPYYGYARQDRK
                     ARSREPITSKLVANMLEVAGVDRLLTVDLHAAQIQGFFDIPVDHLMGAPLIADYFDRH
                     GLVGEDVVVVSPDHGGVTRARKLAQFLQTPIAIIDKRRSVDKMNTSEVMNIIGNVSGK
                     KCILIDDMIDTAGTICHAADALAEAGATAVYASCTHPVLSGPALDNIQRSAIEKLIVL
                     DTIYLPKERLIDKIEQISIADLVAEAIIRIHEKRPLSPLFEMGN"
     gene            2069..2824
                     /gene="recO"
                     /locus_tag="VGT87_001680"
     CDS             2069..2824
                     /gene="recO"
                     /locus_tag="VGT87_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEQ3814510.1"
                     /translation="MQLTESLGIVLFNRNYREDDKLVKIFTEVAGKQMFFVKHISRSK
                     MSSIIQPLTIADFIFKLNDTGLSYVVDYSNVNTYRYINNDIFRLAYASYVLALADAVI
                     ADNESDSHLFTFLKKTLDLMEEGLDYEILTNIFEIQILDRFGISLNFHECAICHRTDL
                     PLDFSHRFSAVLCSEHYYKDNRRNHLDPNVIYLLSRFQKITFDDLRTISLNKDIKKKL
                     RQFIDELYHDYVGIKLKSKTFIDNLVKWGDIMK"
     gene            2927..3934
                     /gene="plsX"
                     /locus_tag="VGT87_001681"
     CDS             2927..3934
                     /gene="plsX"
                     /locus_tag="VGT87_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEQ3814511.1"
                     /translation="MKRIAIDAMGGDNAPKAIVEGVNQAIEAFSDIEIQLYGDQTKIN
                     SYLIQSDRVAIIHTDEKIMSDDEPAKAVRRKKKASMVLAAKAVKEGKADAIISAGNTG
                     ALLAVGLFVVGRIKGVDRPGLLSTIPTVTGLGFDMLDLGANAENTAKHLHQYAILGSF
                     YAKNVRGIANPRVGLLNNGTEETKGDSLRKATYELLTADNTISFVGNVEARELMSGVA
                     DVIVSDGFTGNAVLKSIEGTAISIMGQLKQIINSGGIKTKIGASLLKSSLYEMKKTLD
                     YSSAGGAVLFGLKAPVVKSHGSSDVKAIFSTIKQVRTMLDTNVVGQLVEEFAKETQVN
                     D"
     gene            3927..4169
                     /locus_tag="VGT87_001682"
     CDS             3927..4169
                     /locus_tag="VGT87_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017228.1"
                     /note="carries the fatty acid chain in fatty acid
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEQ3814512.1"
                     /translation="MTRQEIFERLINLIQKQRSYLSVAITEQTHLKNDLAVDSIELVE
                     FIINVEDEFHIAIPDEDVEDMVFMGDVLDYLVQRLT"
BASE COUNT         1343 a          638 c          886 g         1319 t
ORIGIN      
        1 cagccattaa agaaaatcaa aatgctttgc gtacacaaca agcagatttg gaagttgcta
       61 aagtcaattt agctattcaa ttggcttctg ctgaagatga aaaagcaagt ttacttaatc
      121 aacaggcggc agcgcaacaa gcagcagcag aagctttagc agcacaacaa gcagcacaag
      181 ctaaagctcg agaggaagct gcacaacaag cagcatctgt tgaagcagca aaatctgcta
      241 ttactccagc accacaagct actccggcag cgcaaagtag taatgctatt gaaccagctg
      301 cactcacggc tccggcatct ccttctgcaa gaccacaagt ttcatatagt tctgttaata
      361 attacccagt aggacaatgt acatggggtg cgaaatcatt agctccatgg gcaggaaaca
      421 attggggtaa cggtggacaa tgggctgcaa gcgctcaagc agcgggctac tctgtaggat
      481 ctacacctat ggtaggagct attgcggttt ggaatgatgg cggttatggt cacgtcgcag
      541 tagttgttga agttcaaagt tcatcaaata ttcgagtgat ggaatcaaac tacagtggta
      601 aacagtatat tgctgaccac cgtggttggt ttaatccaac aggtgttaca tttatttatc
      661 cacactaaca tattatttag gttagaacag ttgttctaac ctttttgatt actcctattg
      721 tggaatagtt ctgagagtat tgacgatatg gttaatttat aagagagttg tttggccaat
      781 cgcttatgta taatataaga gaagtatata aagtcgataa aaggtatatt taatacattc
      841 tttaacggtt tttcaatttc atttgataat tcacttgaaa agcgttaaaa tggtatagga
      901 ttaaaacttt ggaggaaaaa atgtcttatt ctgatttaaa attgtttgct ttatcgtcaa
      961 acaaggaatt agcagaaaaa gttgcgtctg caatgggtct tcaacttgga aagtctacgg
     1021 ttcgccagtt ttcagatggt gaaatccaag taaatattga agaatcaatt cgtggtcatc
     1081 atgtctttat tttgcaatca actagctcac ctgtcaatga caatttaatg gaaattttga
     1141 ttatggtcga tgctttaaag cgtgctagtg ctgaaaaaat ttcagttgtg atgccttact
     1201 acggctatgc acgccaagac cgtaaagcgc gttctcgtga acctattact tcaaaattag
     1261 ttgctaatat gttagaagtt gctggtgtag atcgcttatt aacggttgat ttacatgctg
     1321 ctcaaattca agggttcttt gatattccag ttgatcactt gatgggtgca cctttaattg
     1381 cagactattt tgatcgtcat ggtttagtgg gtgaagatgt tgtcgttgtt agtcctgacc
     1441 atggtggtgt gactcgtgcg cgtaaattag cacaattcct ccaaacacca attgcaatta
     1501 tcgataagcg tcgtagtgtg gataagatga acaccagtga agtgatgaat attatcggaa
     1561 atgtttcggg caagaaatgt atcttgattg acgatatgat tgataccgca gggactattt
     1621 gccatgcagc agatgcgctt gctgaagcgg gggcaacagc tgtgtatgcg tcatgcacgc
     1681 atcccgttct ttctggacca gcacttgata atattcaacg ttcggctatc gaaaaactaa
     1741 ttgttcttga tactatttat ctgccaaaag aacgtcttat tgataaaatt gaacaaattt
     1801 caattgctga tttggttgca gaagcgatta ttcgtattca tgaaaaacgt ccgttgtcac
     1861 ctttatttga gatgggcaat taagcttaac gaagcctgag ataatagatc tcgggctttt
     1921 tgacttgaat aaaatgtaag ggtaatgtgg tattaattat tgtgggatga actatgcaat
     1981 tctaaaggat tttgggtata ataagaaaga taacaacagt ttattgtaaa ctaagataaa
     2041 aggtaagaga aaaatacgac gaggtaatat gcaactaaca gaatcactag gcattgttct
     2101 ttttaatagg aattatcgag aagatgataa attagtcaaa atatttactg aagtagcagg
     2161 taagcagatg tttttcgtga aacatattag tcgttccaaa atgtcctcaa tcattcaacc
     2221 actaacgatt gctgatttta ttttcaagtt aaatgataca ggcttgtctt atgttgttga
     2281 ctatagtaac gttaacactt atcggtatat taataatgat atttttcgat tagcctatgc
     2341 tagttatgtc ttagcattag ctgatgctgt gattgcagat aatgaatcag attcgcattt
     2401 gtttacgttt ttgaaaaaaa cacttgattt gatggaagag ggcctagatt atgaaatttt
     2461 gacaaatatt tttgaaattc agatattaga tcgttttggt attagtctaa actttcatga
     2521 gtgtgccatt tgtcatcgca ctgatttacc gcttgatttt tcccatcgtt tttcagctgt
     2581 actttgttct gaacattatt acaaagacaa ccgacgtaat catttagatc caaatgttat
     2641 ctacttgttg agtcgatttc aaaaaatcac atttgatgat ttgagaacta tttcattgaa
     2701 taaagacatc aaaaagaagc ttcgtcagtt cattgatgag ttgtatcacg actatgtagg
     2761 aatcaaatta aaaagtaaaa cattcattga taatttagtt aagtggggag atattatgaa
     2821 atgatagggc ctctttcgtt tatgggaatt ggtttcagaa tatgctataa taaaaatact
     2881 attaatagat tgtgtaagaa cacaagatat aattaaagga gtggaaatga agagaattgc
     2941 aatagatgct atgggtggag ataacgctcc taaagctatt gttgagggtg ttaatcaagc
     3001 aatagaagca ttttcagata tcgaaattca actttacggt gatcagacaa agattaactc
     3061 ctatcttatt caatcagatc gtgttgctat tattcataca gatgaaaaaa ttatgtcaga
     3121 tgatgaacct gcaaaagctg ttcgccgtaa aaagaaggct tcaatggttt tagcagctaa
     3181 ggcagtgaaa gaaggaaaag cagatgccat tatttcagca gggaatacag gtgctctttt
     3241 agctgtaggt ttgtttgttg tggggcgtat caagggagtt gatcgtccag gattattatc
     3301 aactattccc acagtaaccg gattaggatt tgatatgttg gatcttggag ctaatgctga
     3361 aaatacggca aaacatttac atcaatatgc tattttaggc tctttctatg ctaagaatgt
     3421 acgtgggatt gctaatccac gtgttggtct attaaacaat ggtacagaag aaacaaaagg
     3481 agattccctt cgtaaggcta cttatgaact tttaacagca gataatacaa tatcttttgt
     3541 tggtaatgtc gaggcgcgag aacttatgtc aggtgttgca gatgttattg tatcagatgg
     3601 tttcacggga aacgcagtgt tgaaatcaat agaaggaaca gctattagca ttatggggca
     3661 actgaaacag ataattaata gcggcggtat taagactaaa attggagcta gtctacttaa
     3721 aagtagtctt tacgagatga agaagactct tgactactct agtgctggag gtgcagtttt
     3781 atttggttta aaggcacctg ttgtaaagtc tcatggttct agtgatgtaa aagctatttt
     3841 ttcaacgata aaacaagtac gtacgatgct agacactaac gtggtcggac aattagtaga
     3901 agaatttgct aaggagactc aagtaaatga ctagacagga gatttttgaa aggctgatta
     3961 acttaattca gaagcaaaga agctatttga gtgtagctat tacagaacaa acacatttaa
     4021 aaaatgattt agcggttgac tctattgaac tagttgaatt tattattaat gttgaagatg
     4081 aatttcatat tgctattcct gatgaagatg ttgaggatat ggtatttatg ggagacgttt
     4141 tagattattt agttcagcgt ttgacttagt tagaataata aaaaga
//
LOCUS       DAUCLR010000111         4084 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.112, whole genome shotgun sequence.
ACCESSION   DAUCLR010000111 DAUCLR010000000
VERSION     DAUCLR010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.112"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..1016
                     /locus_tag="VGT87_001683"
     CDS             <1..1016
                     /locus_tag="VGT87_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEQ3814513.1"
                     /translation="RGGPSKMGRKRKKNLEEMTEVERLQKELEYPRAENAVLKKLREY
                     RLRRRSKTQRATEIIQALRNQFPLEMLLEILDLSRSTYYYQVKRLAQGDKDIELKHVI
                     REIYDEHKGNYGYRRIHMELRNRGFVVNHKKVQRLMKVMGLAIRIRRKRKYSSYKGEV
                     GKKADNLIKRHFEGSKPYEKCYTDVTEFALPEGKLYLSPVLDGYNSEIIDFTLSRSPN
                     LKQVQTMLEKTFPADSYSGTILHSDQGWQYQHQSYHDFLESKGILPSTSRKGNSPDNG
                     MMESFFGILKSEMFYGLETTYQSLDKLEEAITDYIFYYNNKRIKAKLKGLSPVQYRTK
                     SFQ"
     gene            complement(1316..2188)
                     /locus_tag="VGT87_001684"
     CDS             complement(1316..2188)
                     /locus_tag="VGT87_001684"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEQ3814514.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDRIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGV
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
     gene            2412..3722
                     /locus_tag="VGT87_001685"
     CDS             2412..3722
                     /locus_tag="VGT87_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEQ3814515.1"
                     /translation="MIHLIIISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLIAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSGSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYIRYSKGRTNSSLTIDQPPVLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYRQTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWAIIIMLCGMSLLMSLAVKSGLVTLIGHLITTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPTLFPIIATISAQSPHIDIRLLTTATIIGALSTNISPFSSAGSL
                     IQLSLPHIEERSLAFKKQILLGVPISLSLALLTIWILMLLASLS"
     gene            3800..3937
                     /locus_tag="VGT87_001686"
                     /pseudo
     CDS             3800..3937
                     /locus_tag="VGT87_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304910.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1237 a          897 c          756 g         1194 t
ORIGIN      
        1 caagaggggg accgagtaag atggggcgta aacgtaagaa aaatttggaa gagatgacag
       61 aggttgaacg tcttcagaag gaattagaat accctagagc ggagaatgct gtgctaaaaa
      121 agctgagaga ataccgcttg agaagacgaa gcaaaactca aagagcaaca gaaatcattc
      181 aagcattaag gaatcaattt cccttagaga tgctacttga aatccttgat ttatcacgct
      241 ccacctatta ctatcaagtg aagcgactag ctcaaggaga taaggatata gaactaaagc
      301 atgtgattcg agagatttat gatgagcata aaggtaatta cggttatcgt cgtattcata
      361 tggagttgcg taaccgagga tttgtggtca atcataagaa agttcaacgt ctcatgaaag
      421 tcatgggcct agcaatccgc attcgtcgga agcgcaagta ttcttcttac aaaggagagg
      481 ttggtaagaa ggctgataat ctgattaaac gtcacttcga aggctctaaa ccttacgaaa
      541 aatgttacac tgatgtgacg gagtttgcct tacctgaagg aaaactctac ttatcacctg
      601 ttcttgacgg ctataacagt gagattattg atttcactct gtctcggtct cctaacttga
      661 aacaggttca aaccatgctt gagaagactt ttccagcgga ttcatacagc ggaactattc
      721 ttcatagtga ccaggggtgg caataccaac accagtccta ccatgatttt ttagagtcta
      781 aaggtatttt gccatccacg tcccgcaaag ggaatagccc ggataacggc atgatggaat
      841 ccttctttgg cattctcaaa tctgaaatgt tttatggact cgagacaact tatcaatcac
      901 ttgacaagct tgaagaagct attacagatt acatttttta ctacaacaac aaacgaatta
      961 aagcaaaact aaaaggactt agtcctgtcc aatacagaac taaatccttt caataattat
     1021 ttgtccaact ttttggggtc agtacactcc tagttaacga ctttgattga ttttattatg
     1081 atgaatcgtg atttctgaaa acaaaatttt cttcatggat ttggcgtgga acttttacaa
     1141 taaaaacact aacctctccc tatcgttttt tcaggtgaat gcttggaatt ggtataataa
     1201 gcgcacacga atcaggtgaa aacagggaaa gtatttgaag aggcgtttaa actatctgac
     1261 ataaaactca ttctagagga aagacatcct ttaaaaagtt agtttatttt gcaacttaga
     1321 catcaaggta ggttaaaccc ttcatggaaa aatcgagact cttagcacta tgggttaaac
     1381 taccactgga gacgtaatca atcgctaaac cacgaaaacg gctaatagtg gtcatatcaa
     1441 tatttccaga acattcaatc cgagaacgtc ctgcaattag agtaatggcc tgttcaatct
     1501 gttccaatga catattatcc aacatgataa tatcaacacc cgccgccgca gcttcttcgg
     1561 cagcagcaag gctttccact tccacctcga ccattttcac aaaaggggca taggcacgcg
     1621 cttgagcaat cgccttttga acactaccta ctgccgcaat gtgattgtct tttagcatga
     1681 tagcatctga taaattaaag cgatgattat agccaccgcc aactctcacg gcatatttct
     1741 caaaaagacg taaattagga gtggtttttc gagtatcaaa taccttaatg cgatcatcgc
     1801 ctaaggcttc tacataagca gctgtcatcg aagcaatccc tgataaatgt tgtaaaaaat
     1861 tcaaggcaac gcgttcacat gttaagaggc ttctcaccga gcctatgatt tctaaaacca
     1921 aatcgccact agtcaaacga tccccatcct taaattgatg aggattctgg aaggtcacct
     1981 cggcatcaaa tagggtaaaa accctttgaa aaacggttag ccccgctaaa acaccagctt
     2041 ccttggcaaa aagcgacacc ttggcttggc catgatgatc aaaaatggca ttggtactgt
     2101 aatcttcgga atgaacatct tctcgcaagg ctgctttcaa tgtatcatct atttgaaaag
     2161 gggttaaatc agttgaaatg attgacatca ctaagtcctc tatttttaat atcacttgta
     2221 agcactagag gcaattgcta ttaacttccg taggaaatca caaacctgaa tacctcattt
     2281 taaattccaa caagtgtcaa gtcaattgta atttttttgt gtataatagt caagtaccaa
     2341 taggaaacag gtggtctttt gagcgccaaa attgccaacc tatttttata ttctaactaa
     2401 aggacaattc aatgattcat ttaataataa ttagcgccat tgctctagcc attggaattg
     2461 gttaccgcac caaaatcaat attggcctgc tggctattgc tttttcttac ctcatcgcaa
     2521 ccactctcat gggattaagc cccaaagaac ttcttcattt ttggccaacc tcactctttt
     2581 ttaccatttt tagtgtctct ctcttttata acgttgcaac aactaacggc actcttgatg
     2641 ttttggctca acacattctc taccgcacac gcacccaccc taacgccctc tacatgattt
     2701 tatacctgat agcaaccctt ttgtctgctt taggtgctgg atttttcact actatggctg
     2761 tttgctgtcc tctagcgatt accctctgtc aaaaagcgga caagcaccct ttgattggag
     2821 ctcaagccgt caattgggga gcttcaggag gggctaattt gataaccagt ggctcaggca
     2881 ttgtctttca aggcctgttt aagcaaatgg gatgggaaga gcaagctttt tcacttggca
     2941 atcatatctt tatcgttagc attatctacc cactcatcgt cttgctttta ttatcttgtt
     3001 acatccgcta cagtaaggga agaacaaact catccttgac tattgaccaa ccaccagtcc
     3061 tcagcaaggt ccaaagacaa accaccttgc tcatgatcag cagcatggtt ttggtttggc
     3121 tttttccctt gttgcatctc attttcccaa acatcgcatg gatagctact tatcgacaaa
     3181 catttgatat tggctttgtc tcaatactca tggtctgcct cgccttaagg ctcaaattag
     3241 ggaagcaaga agccattttg gctaaggttc cctgggctat aattattatg ctctgcggca
     3301 tgagcttact aatgtctcta gcggtcaagt caggtttagt cacactgata gggcacctca
     3361 taacaacaac cattcctcat ttctggttac ctctgttctt ttgtgtgata gccggagtga
     3421 tgtcactctt tagttccacc ctatccgttg tagccccaac tctctttccc atcattgcta
     3481 ccatcagtgc tcaaagtcct cacattgata ttcgcttact cacaacagcc actattattg
     3541 gcgctctatc tactaatatc tctccttttt catcagctgg ttccctcatt caactctccc
     3601 ttccccatat agaggaacgc agtcttgctt tcaaaaaaca aattcttctg ggtgtgccca
     3661 tcagcttaag tttggcactc ttgaccatct ggattctcat gctgctagct tccttaagtt
     3721 agatttattg tctaacttaa gtttggactt ggtatactaa agtagacaca aaattaagtg
     3781 gaaacgagga aaagtcatta tgccaagaaa aacctttgat aaagccttca aactctctgc
     3841 tgtaaaactc atccttgagg aagagcagcc tgtaaaaatg gttagttcaa ctttaaaaat
     3901 tcatcccaat agtctttatc gatggattca agaatatgaa aaatatggag aaagtgcgtt
     3961 cccaggacat gggagcgcac ttcgtcatgc tcaatttgag ataaaaaaac ttgaaaaaga
     4021 gcacaaactg ctacaggagg aattagctct tctaaaaaag ttccaggtct tcttgaagcc
     4081 aaac
//
LOCUS       DAUCLR010000112         4047 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.113, whole genome shotgun sequence.
ACCESSION   DAUCLR010000112 DAUCLR010000000
VERSION     DAUCLR010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.113"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            10..381
                     /locus_tag="VGT87_001687"
     CDS             10..381
                     /locus_tag="VGT87_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ3814516.1"
                     /translation="MSWKFDEKSPIYAQIAQHVMMQIISQEIKSGDQLPTVREYAEIA
                     GVNPNTMQRAFTELEREGMVYSQRTAGRFVTDDQKLIARKRRELAISELESFITNMTK
                     MGFSHTEIIPVLTSFLKEDSQ"
     gene            381..1079
                     /locus_tag="VGT87_001688"
     CDS             381..1079
                     /locus_tag="VGT87_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ3814517.1"
                     /translation="MAHLLQLHHVSKSYREKKAIDDLTITIPNGKIIGLLGPNGSGKT
                     TLIKLINGLLQPNKGEIVIDGYRPCVETKKIISYLPDTTYLNENMRIKDMLEFFSDFY
                     SDFDKSKATSLLRDLELDPEDRFKTLSKGNKEKVQLILVMSRKARLYVLDEPIGGVDP
                     AARDYILKTIINSYCENASVIISTHLISDIEPILDEVIFLKQGRLFLSGNADDLRQEY
                     QQSIDSLFRETYKV"
     gene            1089..1874
                     /locus_tag="VGT87_001689"
     CDS             1089..1874
                     /locus_tag="VGT87_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ3814518.1"
                     /translation="MFGKLLKYEFRSIGKWYFALNAFVIAIAAILSFTIKLFAQSNSD
                     GLFGVLTNKMLPLTLGLTFGSLIAGSLLSTLLIIIKRFSKSVFGREGYLTLTLPVNSH
                     QIILSKLLASFICSVFNTIILAFAIAIVIVPMFNVNELLEGFFNSFKMDYFINMLTVL
                     AYVLLSTFTSILLIYLSISIGQLFSNRRGLMAFIAYFILVILISVAATYVHNHIFNIN
                     TSADSFPFTEQKTTYLLILEQFIEMIMFYLATNFIIKNKLNLQ"
     gene            complement(2001..2615)
                     /locus_tag="VGT87_001690"
     CDS             complement(2001..2615)
                     /locus_tag="VGT87_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEQ3814519.1"
                     /translation="MTKRNSKAYILWQKIIKILGIIALIGTFFLAFWLYRLGILNDSN
                     ALKDLVQRYRLWGPFVFIVVQIIQIVFPVIPGGLTTVAGFLIFGPVTGFIYNYVGIII
                     GSIVLFLLVKTYGRKFILLFVNDKTFYKYERRLETPGYEKLFIFCMASPVSPADIMVM
                     ITGLTDMSLKRFVTILLITKPISIIGYSYLFIFGKDVISWFLQH"
     gene            2707..3054
                     /gene="ssrA"
                     /locus_tag="VGT87_001691"
     tmRNA           2707..3054
                     /gene="ssrA"
                     /locus_tag="VGT87_001691"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            3356..3976
                     /locus_tag="VGT87_001692"
     CDS             3356..3976
                     /locus_tag="VGT87_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3862 domain-containing protein"
                     /protein_id="HEQ3814520.1"
                     /translation="MTKKHLLTLLLISFFTSFLVACSTTKDKEPQPSDSEIITPRLHQ
                     AAHQDKRANFEKIKLATVDSSFTGGTSLEELISLFGEPNQHDPKTAGEVTIDAYTWQF
                     DQVTLTVNLYQNSSIVKTISNFTFARELGLSQKEYQQLQKGMSYEDVKKILTEPDNYS
                     QASSSDHQTLQAIWVSGLKTDTSGANISLVFENNQLTEMSQVGLEE"
BASE COUNT         1381 a          734 c          649 g         1283 t
ORIGIN      
        1 ggagaccaca tgtcttggaa atttgatgaa aaatctccta tttatgcgca gatagcacaa
       61 catgtcatga tgcaaattat tagccaagaa atcaagagtg gtgatcaact tccaactgtt
      121 cgtgaatatg cagaaatcgc aggtgtcaat cctaacacca tgcaacgagc gtttacagaa
      181 cttgaacggg aaggaatggt ttattcgcaa cgtactgctg gtcgttttgt gactgacgat
      241 caaaaattaa ttgctcgtaa aagacgagag ttagcaatta gtgaattaga atcatttatc
      301 accaatatga caaaaatggg atttagtcac actgagatta tccctgtttt gacgagcttt
      361 ttaaaggagg attcacaata atggcacacc tattgcaact acatcatgtt tcaaaatctt
      421 accgtgaaaa aaaagctatt gatgacttaa ccattactat cccgaacggt aaaattatcg
      481 gattattagg tccaaatgga agtggtaaaa caactttaat taaactcatt aatggccttt
      541 tacaaccaaa taaaggagaa attgtcattg atggctaccg tccctgtgtt gagacaaaaa
      601 aaattatttc ttatttacca gatactactt atctcaatga aaatatgaga attaaggaca
      661 tgttggagtt ctttagtgat ttttacagcg actttgacaa atccaaagca acaagccttc
      721 taagagattt agaactagac cctgaagacc gcttcaaaac tctatccaaa ggaaacaaag
      781 aaaaagtaca attaattctt gtgatgagtc gtaaagccag actctatgta cttgatgaac
      841 ctattggtgg agttgaccct gccgcaagag actacatcct aaaaaccatc attaatagtt
      901 attgtgaaaa tgcttctgtt attatctcaa cccacttgat ttcggacatt gaacctatcc
      961 ttgacgaagt cattttcttg aaacaaggaa gacttttctt atctggcaat gctgatgatc
     1021 taagacaaga ataccagcaa tccattgatt ccctctttag ggaaacttat aaggtttagg
     1081 aggatatcat gtttggaaaa ttattaaaat acgaatttag atctattgga aaatggtact
     1141 ttgcgcttaa tgctttcgtt attgccatcg ctgccatttt gtcatttacg ataaaactgt
     1201 ttgctcaaag caatagtgat ggactatttg gagtactaac caataagatg ttgcctctga
     1261 cattaggttt aacttttggc tccctgatcg ctggctccct cttatcaaca ttacttatca
     1321 ttatcaaacg ttttagcaaa agtgtttttg gacgggaagg atacttaacg ttgactttac
     1381 ccgtcaattc gcatcaaatt attttatcaa aactactagc ttcttttatt tgcagtgttt
     1441 tcaatactat catccttgca tttgctatcg ctattgtaat tgtaccaatg tttaacgtca
     1501 acgaactatt agaaggattc tttaatagtt ttaagatgga ttatttcatc aatatgctaa
     1561 ctgtactagc ctatgtccta ttatcaacat ttacgagtat cttattaatt tatctttcca
     1621 tttctatagg tcaacttttt tctaatcggc gaggcttgat ggcctttatt gcatatttta
     1681 tattagttat tctgattagc gttgctgcaa catatgttca caatcacatc tttaatatta
     1741 atacaagtgc cgatagtttc ccatttactg agcaaaaaac aacttatctt cttattttgg
     1801 aacaatttat tgaaatgata atgttttacc tcgccactaa ttttattatc aaaaataaac
     1861 tcaacctaca gtaagcgtta agactttctt ttaaactatg acacactata gtttaaaaga
     1921 aagttttttt cagtgttcat agtaaattaa aaaaccgtct tccatcaaat agaagcggtt
     1981 tatcaaatta acaccaaacc ttaatgctgt aagaaccaag atataacatc ttttccaaat
     2041 ataaataaat aactgtagcc gataatagaa attggtttag ttattaaaag aatagttaca
     2101 aagcgcttta aagacatgtc cgtcaatccc gtaatcataa ccataatatc agctggagaa
     2161 acaggcgatg ccatacaaaa aataaataat ttttcatatc ctggtgtctc caaacgcctt
     2221 tcatacttgt aaaatgtttt gtcatttaca aagagtaaga taaatttacg accataagtc
     2281 ttaacgagca agaagaggac tatactgcca ataatgatgc ctacataatt ataaataaaa
     2341 cctgttacag ggccaaagat taaaaaacct gcaactgtcg taaggcctcc aggaatcaca
     2401 ggaaaaacaa tctgtatgat ctgcacgaca ataaaaacaa acggccccca taatctatag
     2461 cgctgcacta agtctttaag agcattacta tcgtttaata tacctagtct atacagccaa
     2521 aaagcgagga aaaaagtacc aattaatgca ataattccta aaattttgat aattttttgc
     2581 cataatatat aggcttttga attacgtttt gtcatagact aatcatacca taagctgtaa
     2641 aattttgcta tttaaaaaaa gttttgatag aatagttagt gtaagaaatt ggttaattat
     2701 ctatttgggg ttgttacgga ttcgacaggc attatgaggc atgttttgcg tcccatcggc
     2761 agatgtaaat tgccagttaa atataactgc aaaaaataca aactcttacg ctttagctgc
     2821 ctaaaaacca gctagcgtga cttctacaag attgcttgtg tcctgttaga agtctcaaaa
     2881 tagcaagcta cggttacgaa attgtctagt ttcgtgacaa gagattgata gactcgcaaa
     2941 ctaatggctt gagttatgtg tctttagttt gttaaatgaa gacataacct atggacgtag
     3001 acaaatatgt tggcaggtgt ttggacgtgg gttcgactcc caccagctcc atcaatgctt
     3061 accgtaagta atcataactt actaaaacct tgttacatca aggttttttc tttttgtctt
     3121 gttcatgagt ttccgttatc cctatagcca taccatcacg cttaccgata ctccatcaat
     3181 accactcaaa accttgtcat atcagagctt tttgaccatt tttttcatga atatctaaaa
     3241 aaaagaatca aaatagtact aaattcccca tagcgcatgc gttatgggga atcatagcta
     3301 tatcttgttt ataatttatg atataataca gcaaaaatag tttaggaaat aacatatgac
     3361 aaaaaaacac ttactaacac ttcttctcat ctcttttttt actagctttt tggtagcttg
     3421 ttcaacaacg aaagataaag agcctcaacc gtctgattca gaaatcatta ctccccgact
     3481 acaccaagcc gctcatcaag ataaacgcgc taactttgaa aaaattaaac ttgcgactgt
     3541 tgattcctca tttacaggag gaacaagcct tgaagaactt atttcactct ttggagagcc
     3601 taaccaacat gatccaaaaa cagcaggcga agtaacaatc gacgcttata cttggcagtt
     3661 tgatcaagtt actctcactg ttaatcttta tcaaaatagt agtattgtta aaaccatctc
     3721 taattttacc tttgcaagag agttaggctt atcgcaaaaa gaataccaac aattacaaaa
     3781 aggaatgtct tatgaagacg ttaaaaagat cttaacagaa cctgataatt atagccaagc
     3841 gtcatctagt gatcatcaaa ctttgcaagc gatttgggtt agtggcttaa agacagatac
     3901 aagcggagct aatatttctc tcgtttttga aaataatcag ttaacagaaa tgtctcaggt
     3961 aggacttgaa gaataaaaaa tgagtaatat attttgctaa aaagattgat ttcggtgtac
     4021 atcgaaatca atctttttat atcattt
//
LOCUS       DAUCLR010000113         3944 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.115, whole genome shotgun sequence.
ACCESSION   DAUCLR010000113 DAUCLR010000000
VERSION     DAUCLR010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.115"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..1809
                     /gene="cadA"
                     /locus_tag="VGT87_001693"
     CDS             <1..1809
                     /gene="cadA"
                     /locus_tag="VGT87_001693"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEQ3814521.1"
                     /translation="CLVLIIIGLAFLHSFPQLASAIFITAFLIGGYASAKTGILDLVK
                     NKHLSVDILMILAAIGAGIIGYWLEGALLIFIFSLSNTLEEMAMEKSKDAISALMSLT
                     PDTARQYQEDGHILEVETRSLSVGDRLQVRKGEAVPIDGQLLSPFGQFDESMVTGEPI
                     TVDKAEGQDLIGGTINQGQTIDMLVTIENDDTLFAKIINLVESAQEKKSKTATFIESL
                     EDGYVKFVLVLIPAFILFSHFVLSWTWLAAFYRGMILLTVASPCALIASSTPASLAAI
                     SRAARKGLIIKGGDIVDNMGDIKAVVMDKTGTLTQGKPSVVNAHYLEDELLVNRLVKG
                     AEAASTHPISKAFLEYTEKLEPLTFDHLEEISGKGFQGFYQGQEWRIGKKSFILEMVP
                     DLSAFEETIQVEESQGKTLIFVSRDNQLVAYYALLDDIKLESKRAIEALHAMGIKTVM
                     LTGDQERTANYVAQKLGIDEVVANCMPQDKVAKLAELKTKYGFVAMVGDGINDAPALA
                     QADVSYAIGSGTDIAMESADSVIMDDLTRIPFSIQLSRKMKTIIKQNIVFALSVITLL
                     ILANVFQVVNLPLGVVGHEGSTILVILNGLRLLSFK"
     gene            complement(2109..3044)
                     /locus_tag="VGT87_001694"
     CDS             complement(2109..3044)
                     /locus_tag="VGT87_001694"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004228450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEQ3814522.1"
                     /translation="MVSTATQISHFSFDNCLMNAAGVYCMTKEELMEVEKSQAASFVT
                     KTGTLKVRPGNPEPRYADTRLGSINSMGLPNNGFRYYLDFVSDLAKTGQHKPHFLSVV
                     GLSPTETETILKAIMASDYEGLVELNLSCPNVPGKPQIAYDFETTDQLLENIFTYYTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLSFVNCVNSIGNGLVIEDEQVLIKPKNGFGGI
                     GGDYIKPTALANVHAFYKRLKPSIHIIGTGGVKTGRDAFEHILCGASMVQIGTALHQE
                     GPAIFERVTKELKTIMVEKGYQSLDDFRGNLRYKD"
     gene            3283..>3944
                     /locus_tag="VGT87_001695"
     CDS             3283..>3944
                     /locus_tag="VGT87_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003104178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEQ3814523.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDLAFTSVLTRAIKTTNLALENAGQLWVPTEKSWRLNERHYGALTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPAMAKDDEYSAHKDRRYADLDPALIPDAENLKVTLERAM
                     PYWEEKIAPALLDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFELDE
                     KL"
BASE COUNT         1189 a          725 c          831 g         1199 t
ORIGIN      
        1 tgtttggtat taataataat tggcctagcc tttttacact catttccaca actggcttcc
       61 gctattttca ttacagcctt cttgattgga ggatatgcgt ctgcaaaaac gggtatattg
      121 gatttggtga agaacaaaca cttgtcagtg gatattttga tgattttggc agctatcggt
      181 gctggaatta tcggctattg gctggagggt gctctgctta tttttatctt ttcgttgtcc
      241 aatacgcttg aagaaatggc catggaaaaa agtaaggatg ctatttcagc cttgatgtcc
      301 ttaacgccag atacagctcg tcaataccaa gaagatggcc atattttaga agttgagacc
      361 agatccttga gtgttggtga tcgtttacaa gttcgtaaag gcgaagctgt tccaattgac
      421 gggcagttgc ttagtccttt tggtcaattt gatgaatcta tggtcactgg tgagcctatc
      481 actgttgata aggcagaagg ccaggatctg attgggggaa ccattaacca aggacaaacc
      541 atagatatgt tggttactat tgaaaatgac gatactctct ttgccaagat tattaatctg
      601 gtagaatctg cccaagaaaa gaaaagcaaa accgccacct ttatcgaaag cttagaagat
      661 ggttatgtca aatttgtgct cgtccttatt cctgccttta tcctctttag ccactttgtg
      721 ctttcttgga cttggttggc tgctttttac cgagggatga ttctcttaac agtagcttca
      781 ccatgtgccc taattgccag ttccacacct gctagcttgg ctgctatttc tcgtgcagcc
      841 aggaagggat tgattatcaa aggaggggat attgtcgata acatgggaga tattaaggct
      901 gttgtcatgg ataaaacggg aacgctcacc caaggaaaac cttctgttgt gaatgctcat
      961 tatttggaag atgagttgct cgtgaataga ctggtaaaag gagcagaggc tgctagtacc
     1021 catcctattt ctaaagcctt tcttgaatac actgaaaaat tggagccact gacctttgac
     1081 cacttagaag aaatttctgg gaaaggtttt cagggcttct atcaagggca agaatggcgc
     1141 attgggaaga aatcctttat tttagagatg gtgccagatc tttcagcttt tgaagaaacg
     1201 attcaggtag aagaaagtca agggaaaact cttatctttg tttctcgcga caaccaacta
     1261 gtagcctact acgctttgtt agatgacatc aaattagagt caaaacgtgc cattgaagct
     1321 cttcatgcta tgggaatcaa gacggtcatg ttaacagggg accaagaacg aactgccaat
     1381 tatgtggcac aaaaacttgg tattgatgaa gtggtagcca actgtatgcc tcaagataag
     1441 gtggctaagt tagcagaatt aaagaccaaa tatggttttg tggccatggt aggagatggt
     1501 attaatgatg ctcctgccct tgctcaagca gatgtttctt atgctattgg atcaggaaca
     1561 gatattgcaa tggaaagtgc agacagtgtg attatggatg acttgactcg tattccattt
     1621 tcgattcaac tttcccgcaa aatgaagacc attatcaaac aaaatattgt ttttgcctta
     1681 tctgtgatta ccttattgat tttagccaat gtttttcagg tagttaactt gccgcttggt
     1741 gttgttggac acgaaggctc aacgatttta gtgattttaa atggcttgcg tttactttct
     1801 tttaaataat ttagaaactt gcttgaaaac ttgaaagtgt ttataataat actaagactt
     1861 atgaaagaga aaaccgccgt atgtttacta ttatgatcat tagtttagct gtattttcaa
     1921 taattaaaat cattctattt ttctattttt atcagcaatt tgagcgcaag caaaaaagaa
     1981 aagttgaatc aaacaaccag caaagacagt ccaaagaaag tgacgctgtc ccttcaaaaa
     2041 aattcccatt ttaaaaagct tggaaagtgt acaaagtgca catttccaag cttttttgac
     2101 tattaaggtt aatctttgta acgtaagttc ccacgaaaat cgtctaagct ttggtagcct
     2161 ttttctacca taatcgtttt tagttcctta gtgacccgtt caaagatagc tggcccttct
     2221 tgatgaaggg ctgtcccaat ttggaccatg ctggcgccac aaagaatatg ttcaaaggca
     2281 tcgcgtcctg ttttaactcc ccctgtacca ataatgtgga tagaaggttt taagcgtttg
     2341 taaaacgcat ggacattggc tagagcagta ggtttgatgt aatctccacc tataccacca
     2401 aagccatttt taggcttgat gaggacttgc tcatcctcaa tgaccagccc atttccgata
     2461 gaattgacac agttaacaaa ggaaaggggg tatttattga aaatcgcagc cgcttgatca
     2521 aaatgcacaa tgtcaaaata aggaggtaat ttgataccaa gaggtttagt gtaataggta
     2581 aagatgttct ctagaagttg atcagtggtt tcaaaatcat aagcaatctg tggttttcct
     2641 ggaacgttcg ggcaagaaag gtttagttcc actagaccct cataatctga agccatgatg
     2701 gcttttaaaa tggtttcggt ttcagtagga gatagcccga caacggagag aaaatgaggt
     2761 ttatgttgcc cggttttggc aagatcactg acaaagtcta agtagtagcg aaaaccatta
     2821 ttagggagtc ccattgaatt gatggaacca agacgagtat ctgcgtagcg aggttctgga
     2881 ttacctggac gaactttaag cgtccctgtt ttggtgacaa aggaagcggc ttgagacttt
     2941 tcaacctcca taagctcttc ttttgtcatg cagtaaacac cagcagcatt catcaaacag
     3001 ttatcaaaag agaaatgact aatttgtgta gcagtagaaa ccattatttc ctccatattt
     3061 tagacaaatt ttttctattc taccactagg catgaataat agcaagctat ttttacaaaa
     3121 atgtaaatat attactgtta ttgacgaaca tttgaaattt tcatgatagt cacattatga
     3181 ttttcaataa agtcagaata gactagcttt tttgctatat tttttgtaaa ataggaattg
     3241 taaaaaggta gcaaccttta aattaaaagg agatattaac tcatggtaaa attggttttc
     3301 gctcgccacg gtgagtcaga atggaacaaa gctaacctct tcactggttg ggcagatgtt
     3361 gatctttcag aaaaagggac acaacaagcg attgatgcag gtaaattgat caaagaagca
     3421 ggtattgaat ttgaccttgc tttcacttca gtattgacac gtgctatcaa aacaactaac
     3481 cttgcccttg aaaatgcagg tcaattgtgg gttccaactg aaaaatcatg gcgcttgaac
     3541 gagcgtcact atggtgcttt gactggcaaa aacaaagctg aagctgcaga acaatttggt
     3601 gatgaacaag ttcatatctg gcgtcgttct tacgacgtat tgccgccagc tatggctaaa
     3661 gatgatgagt attcagcaca caaagaccgt cgttatgctg atcttgaccc agcccttatt
     3721 ccagatgctg aaaacttaaa agtaactctt gaacgcgcaa tgccttactg ggaagaaaaa
     3781 attgctccag ctcttcttga cggtaaaaac gtctttgttg gcgcacatgg taactcaatc
     3841 cgcgctcttg tgaaacacat taaaggtctt tcagatgacg aaatcatgga tgtggaaatt
     3901 ccaaacttcc caccacttgt ttttgaatta gatgaaaaac ttaa
//
LOCUS       DAUCLR010000114         3940 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.116, whole genome shotgun sequence.
ACCESSION   DAUCLR010000114 DAUCLR010000000
VERSION     DAUCLR010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.116"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..1783)
                     /locus_tag="VGT87_001696"
     CDS             complement(<1..1783)
                     /locus_tag="VGT87_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEQ3814524.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFLIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRV"
     gene            complement(1767..2057)
                     /locus_tag="VGT87_001697"
                     /pseudo
     CDS             complement(1767..2057)
                     /locus_tag="VGT87_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            2926..3375
                     /locus_tag="VGT87_001698"
     CDS             2926..3375
                     /locus_tag="VGT87_001698"
                     /inference="COORDINATES: protein motif:HMM:NF013698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEQ3814525.1"
                     /translation="MKEEVKITMEATGKYHLPILYELKDKDYFVTVINPLKMKQFCRA
                     LNFRKAKNDKIDAKQIAEYGLMYWKELEEYKIDEVNYRILKELNRSYQHYMELRIDQM
                     NYIDQTIHRTFPGIKKLIPHNSGDFSKDKLLDFLEKWWHKDLVLEKT"
     gene            3580..3933
                     /locus_tag="VGT87_001699"
     CDS             3580..3933
                     /locus_tag="VGT87_001699"
                     /inference="COORDINATES: protein motif:HMM:NF014430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEQ3814526.1"
                     /translation="MIEISEPLEEYKEAKKFSGISEKLAVQIVAEFGDLSKFKNKKSL
                     ISFIGIDAPPYESGNFKADQRKISKRGNAILRKVGYQAMKCMMSAKNPENEIYKYMIK
                     KEKEGKAKTFANLQA"
BASE COUNT         1430 a          703 c          712 g         1095 t
ORIGIN      
        1 caacccgtaa ttcctcaatc actttcatca accctcgtac ttcactattg gttactgcac
       61 gaatggcttt tctaaggatt gggaagcgtt ccccatcacg agaggaaatc cccgtaagga
      121 atgtcagaat atcaatagcc agtgattcag aatctttggg atttttcata atcacataag
      181 ggtcaagtaa gcctttgttt ttctcatcag aagtcagagt gacgatattg atttcatggg
      241 aaatctctgg caaggtttct ttccatctgc cacgttctgc ttttgggtct acaatcactg
      301 cttgtgcccc ataaagcacc gcataataga cgataaggtt attcgcaaag gatttaccac
      361 cacccagcga accaacaaaa gccgacgcta acgcattggt tactgaaccc ttaacccctt
      421 gactggcaag agcaggtttc agatagacat tgcgtccagt atctaagctg tagccaacat
      481 aaatcccctc attttccccc agcatttgag tagcaccaaa acctaaacca gcgaggaaat
      541 cagaggtcac gtattgaata taatcattca tataacgctt gctggcaggt aaaaattctt
      601 catgtaagcc gagcatatcc ccaaatggtc gtaccagttt tacgcttaaa tcgtcataaa
      661 aatctttcac ttcattacaa cgacgtttga gttcgtcaag atcatttgct gataccctta
      721 ccacataaga cagcttgtac atagattcct tgctttggtc taaattggtt tccagctcat
      781 tcacactttc cagagcttcc gccacattgg agctggtttc attatcactt tgccaagcgt
      841 ggttatccaa gtctttcagt tctttctttt tattgcggac agtagatagg gctttacgat
      901 tcgctacaat ttccacattc attgacgtat caatcgggaa tgtaaattgc tgttgctggt
      961 agtagaagat ttcagaggac gggaagtcca gttctccgac aatgctgtta atggtaaagt
     1021 aagctacata gacggtttca tcttcctgct ggattttcaa atatcgctgt ttttcttcca
     1081 ccaaacagcg agtaggctta atcaagtcat agtatttaat cagcgtttca ttatccagct
     1141 ttttctttga tagatggtac tcatactctt cataggcagt gcctgtctgt ccgtaaaggt
     1201 gttcaatcag atagccgaag tcgtccttat ctaacctgcg gattttgaaa cgacgagaga
     1261 ttttattttc taagagcttt tccatcttct gaaaacgcag gatttcatca ttactcatac
     1321 taacaaaatc gcccatcagc ttatggttca catcatagac aaaatcagac aaagcatttt
     1381 ttgcttcaac ggtaagactt ttcatagaaa actcctgatc gttgagaagc aacttaaagc
     1441 cgataaggaa acggtagttc acttgatttt cgccaatcat ggatattaaa gcgtctgtct
     1501 gttggtcgat tttgtcatag gcaaccgctt tgagcttgcc agtgacttca tttttggaac
     1561 gctcttgtgc agaacgtatg ctggattctg tactgatttg taaagcatga attttgccat
     1621 cacgattttg tgcgataagc tgtctgaaag aatcatgcac ttgtattttc tgttctggac
     1681 ttagaaatga gtaattgtaa ggaacaagct catagtaagc ataacattcc ccgtctttat
     1741 tccagacgag attgttttca atgtatttaa ttggatatgc cataaaattc actcctaact
     1801 gctgtaatgg cttcttgtgg ctggtttctg ccaagcgtta ctttttttcc tgcataggtc
     1861 agctttggtc gcagtgcata agcaatgaca gacttcaaaa atccataagg ctttttacca
     1921 tcaaaagttt ttgtagacat aaaccatgtg aaagccacag gaatcccaaa gtatttgaga
     1981 aatgctccct ctatcatgga aagagggggc aagttgccaa gtatcatcac tgcaaagagt
     2041 gacacgacaa accatgtgga gacggtggta ttgatgtcaa ggaaaataag ataataggct
     2101 ataaaagtgt tgaaattaat ggttttgggg ttttagaaga aatcctaaaa gccatttttc
     2161 tatttagata aatatcttaa ttataattaa agaataaatt gaggatcaag aacctcaata
     2221 ttatatagaa aatagccacg aagcgatcat accaaaagaa atcttcatgc aagtctaaga
     2281 agaaatggat agacgagcta atatggtaag tggcaaaggg aaaagaagaa tctattccag
     2341 taaatacgcc ctttcaagta ttgtctactg ctctatatgt ggggatattt acaggagaat
     2401 agcatggaat aatagaggaa agaaaaaaac aagttattta tagtgttaaa ttaccaacac
     2461 tcttattact tcctatgtaa tgtttacagt tctagtatca taacttgttc aattagagga
     2521 tttaaacaga tttttgtggg ctgtttttta gtaccataaa tagtgtaaga aaggaagtaa
     2581 ttcctatctt gcactatttt tgtataatga agaagtagta tgacgaggct gttttagggt
     2641 tgaaacatcc gccacaaaaa ctgtcaacta ccttttcatt attcatattc ggttaagcag
     2701 gttgggcaaa acgcccgtgt cacaagctaa aaagagtgta atggtgcagg tagaaactac
     2761 aaaataaaaa gaaagaggaa aaaacatgat atctgtagga atagacatat caaaagaaaa
     2821 aatcacagtt tgtgcattag gacaaggaag caaaataatc tggaaaccct ttgatgtttc
     2881 tctaaaaaaa cagaagtaga aaaattttta gagaaactag aaaaaataaa agaagaagta
     2941 aaaataacaa tggaagcaac aggaaaatat catctcccaa tactttatga attaaaagat
     3001 aaagactact ttgtaacagt aataaatcca ttaaaaatga aacaattctg tagagcatta
     3061 aactttagaa aagcaaaaaa cgacaagata gatgcaaaac aaatagcaga atatggacta
     3121 atgtattgga aagaattaga agaatacaaa atagatgaag tgaactacag aatcctaaaa
     3181 gaattaaata gaagctacca acattacatg gagctaagaa tcgaccaaat gaactacata
     3241 gatcaaacaa tacatcgaac atttccaggc ataaaaaaac taataccaca taattcagga
     3301 gacttttcaa aagacaaact attggacttt ctagaaaaat ggtggcataa agacctagta
     3361 ttagaaaaaa cataagaaga atttgtagaa gaatataaaa cttgggcaaa ggaaaagaga
     3421 taccatccaa atgcaaacaa agcaaaaacc atttacaaaa tagcagagga tagtatctca
     3481 acacaacctt ctcataactc aaaactagaa acgaatatca gagaaggaat aaacctgata
     3541 aaacaaataa atcagattct aaatagaatt ttatcacaaa tgatagaaat atccgagccc
     3601 ttagaagaat ataaagaagc aaaaaaattc tcaggcatat cagaaaaatt agcagtacaa
     3661 atagtagcag aatttggaga cctatcaaaa tttaaaaaca aaaaaagcct aatatccttt
     3721 ataggaatag acgcaccacc atatgaatca ggaaacttta aagcagatca aagaaaaata
     3781 agtaaaagag gaaacgcaat actaagaaaa gtaggctacc aagcaatgaa atgtatgatg
     3841 agtgctaaaa atccagaaaa tgaaatatat aagtatatga taaaaaaaga aaaagaaggg
     3901 aaagccaaaa cgtttgcaaa tttgcaggct taaacaaatt
//
LOCUS       DAUCLR010000115         3900 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.117, whole genome shotgun sequence.
ACCESSION   DAUCLR010000115 DAUCLR010000000
VERSION     DAUCLR010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.117"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            287..1330
                     /gene="pheS"
                     /locus_tag="VGT87_001700"
     CDS             287..1330
                     /gene="pheS"
                     /locus_tag="VGT87_001700"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019314825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEQ3814527.1"
                     /translation="MDLQAQLEELKTKTLETLQSLTGNHTKELQDLRVAVLGKKGSLT
                     ELLKGLKDLSNDLRPVVGKQVNEVRDLLTKAFEEQAKIVEAAKIQAQLDAESIDVTLP
                     GRQMTLGHRHVLTQTSEEIEDIFLGMGFQIVDGFEVEKDYYNFERMNLPKDHPARDMQ
                     DTFYITEEILLRTHTSPVQARTLDQHDFSKGPLKMVSPGRVFRRDTDDATHSHQFHQI
                     EGLVVGKNISMGDLKGTLEMIIKKMFGEERSIRLRPSYFPFTEPSVEVDVSCFKCGGK
                     GCNVCKKTGWIEILGAGMVHPSVLEMSGVDAKEYSGFAFGLGQERIAMLRYGINDIRG
                     FYQGDQRFSEQFN"
     gene            1540..>3900
                     /locus_tag="VGT87_001701"
     CDS             1540..>3900
                     /locus_tag="VGT87_001701"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEQ3814528.1"
                     /translation="MLVSYKWLKELVDIDVTPAALAEKMSTTGIEVEGIEVPADGLSK
                     LVVGHVLSCEDVPETHLHLCQVDTGDETPRQIVCGAPNVKAGIKVIVAVPGARIADNY
                     KIKKGKIRGMESLGMICSLQELGLSDSIIPKEFSDGIQILPEEAVPGDAIFKYLDLDD
                     HIIELSITPNRADALSMRGVAHEVAAIYGKSVSFPQKNLQESDKATSEAIEVAIASDK
                     VLTYASRVVENVKVKPSPQWLQNLLMNAGIRPINNVVDVTNYVLLYFGQPMHAFDYDK
                     FEDHKIVARAARQGESLVTLDGEKRDLTTEDLVITVADKPVALAGVMGGQATEIDGNS
                     QTVVLEAAVFDGKSIRKTSGRLNLRSESSSRFEKGVNYATVLEALDFAAAMLQELAEG
                     QVLSGHVQAGQLPTEPVEVSTSLDYVNVRLGTELTFADIRRIFDQLGFGLTGDETSFT
                     VAVPRRRWDVSIPADLVEEIARIYGYDKLPTTLPEAGGTAAELTPTQALRRKVRGLAE
                     GLGLTEIISYALTTPEKAVEFAVAPSHLTELMWPMSVERSALRQNMVSGMLDTVAYNV
                     ARKQSNLALYEIGKIFEQEANPKEDLPNEVNHFAFAICGLVAQKDFQTQAQVVDFYHA
                     KGILDTLFANLNLKVQYVPTKDLASMHPGRTALILLDEQVIGFVGQVHPGTAKAYSIP
                     ETYVAELDMAALEAALSSDQTFVEITKFPAMTRDVALLLDREVSHQAIVTAIESAGVK
                     RLTKIKLFDVYEGVTIQAGKKSMAYSLTFQNPNDNLTDEEVAKYMEK"
BASE COUNT         1158 a          704 c          920 g         1118 t
ORIGIN      
        1 ggcgtggaaa agtcactgat ttaagattgt cactaagcac tttttttgat agaatagaac
       61 taattgaata agtgtctatg agactagtac taatagagaa attaacaggg agtgagggcc
      121 gtgactggaa gccttcttaa gggaactatt aggaattcac tcagctaaag acaattaatt
      181 aaggtctgat aacatcagat aaaaacggat ggtaccgcgt gtcaacgctc cgcttatgaa
      241 gtggagtagt ggcacgcttt tttcatcgct aaaagaaggg aaaactatgg atttacaagc
      301 acaattagag gaattaaaaa caaaaacatt ggagactctc caatctttga cggggaatca
      361 taccaaagaa ctgcaagatt tgcgcgtggc tgttcttggg aaaaagggat ctttgacaga
      421 attactaaag gggctaaaag acttgtctaa tgacttgcgt cctgtggttg gtaaacaggt
      481 gaacgaagtt cgtgacttat tgacaaaagc ctttgaagaa caggctaaaa ttgtagaagc
      541 tgctaaaatt caagcccagc ttgatgctga aagcattgat gtgactttac cgggtcgtca
      601 aatgactctg ggccaccgtc atgtgttaac acaaaccagt gaagaaatcg aagatatttt
      661 cttaggaatg ggttttcaga ttgttgatgg ctttgaggta gaaaaagatt attacaactt
      721 tgagcgcatg aatttgccaa aggatcaccc tgcccgtgac atgcaagata cgttttatat
      781 cacagaagaa atcttgttac gaactcacac cagtcctgtg caggcccgta ctcttgatca
      841 acatgatttt tcaaaagggc ctttaaaaat ggtttctcca ggtcgtgttt tccgtcgaga
      901 caccgatgat gctacccact cacatcaatt ccatcaaatt gaaggtttgg tcgtgggcaa
      961 aaatatttcc atgggagacc tcaaagggac tcttgaaatg atcatcaaga agatgttcgg
     1021 tgaagaacgt agcattcgtt tgcgcccgtc atatttcccg ttcacagaac catctgttga
     1081 agtggatgtt tcttgcttta aatgtggtgg gaaaggctgt aatgtctgca agaaaacagg
     1141 atggattgaa attcttggtg ctggtatggt tcacccaagt gtcttggaaa tgtcaggtgt
     1201 tgatgcaaaa gagtattcag gctttgcctt tggtttaggt caagagcgga ttgccatgtt
     1261 gcgttatggc attaatgaca ttcgtggttt ctatcaaggc gaccaacgtt tctctgaaca
     1321 atttaactaa aatgagtcgt ttgaagactt ctgtcagcta ttttatctaa gattgagtga
     1381 aagttgatag gtaatgataa taggttaatt gtctaagggg aaagtccctc tcgcttcaac
     1441 gtcataaggg ttttgacttg gaacttaaaa tacgaaagga aatgactcaa atcacggctt
     1501 gagagctttc tctagccgag tgatttgggc actaaaatta tgttagtatc ttataaatgg
     1561 ttaaaagaat tagtagatat cgatgtgaca ccggcagcac ttgctgagaa aatgtcgaca
     1621 acgggtatcg aggtagaagg aattgaagtc cctgcagatg gtttatcaaa actggttgtg
     1681 gggcatgtgc tttcttgcga agatgtgcca gagacacatt tgcatttgtg tcaggtagat
     1741 acaggtgatg aaacgccacg tcaaattgtg tgtggcgcac caaatgtgaa agcagggatt
     1801 aaggttatcg tggctgtgcc aggtgctcgt attgcggata actataaaat caaaaaaggc
     1861 aaaatccgcg gcatggaatc tcttggcatg atttgttcct tgcaagaact tggtttgtca
     1921 gatagtatta ttccaaaaga attttcagat ggtattcaaa tcttgccaga ggaggctgtg
     1981 ccaggtgatg ctatttttaa atacctggac ttggacgatc atatcattga attgtccatt
     2041 acacctaacc gtgctgatgc gctttctatg cgaggtgtgg cacatgaagt cgcagctatt
     2101 tatggcaagt cagtatcatt cccccaaaaa aatctacaag aatctgataa ggcaacttca
     2161 gaagccattg aagtggccat tgcctctgat aaagttctaa cttacgctag ccgtgtcgtt
     2221 gaaaatgtca aagttaagcc aagtccacaa tggttgcaaa accttttaat gaatgctggt
     2281 attcgtccta ttaataatgt ggttgatgtg accaactatg tgctcttgta ttttggtcaa
     2341 cctatgcatg cttttgatta tgataaattt gaagaccata agattgtggc gcgtgcggct
     2401 cgtcaagggg aaagtttagt cacacttgat ggtgaaaaac gggatttgac cactgaggat
     2461 cttgttatta ccgtggctga caaaccagtt gctttagcag gtgttatggg tggtcaagcc
     2521 actgaaattg atggcaactc tcagacggtt gttcttgaag ctgctgtttt tgatggcaaa
     2581 tcgattcgta aaaccagtgg tcgtttaaac cttcgttcgg aatcctcttc acgttttgaa
     2641 aaaggggtta actatgcaac tgttttagaa gcgcttgact ttgcagcggc tatgttacaa
     2701 gaattagcag aaggtcaagt cttatcaggt catgttcaag ctggacaatt gccaacagaa
     2761 cctgttgaag tctcaactag ccttgactat gtgaatgttc gtttaggaac agaattaact
     2821 tttgctgata ttcgaaggat ttttgaccaa cttggatttg gtctaacagg agatgaaaca
     2881 agcttcacgg tagctgttcc tcggcgtcgt tgggatgtta gcatcccagc tgacttggtg
     2941 gaagaaattg cgcgtatcta tggttatgat aagttgccga caaccttacc agaggcaggg
     3001 ggaacggctg cagaattaac gccaacacag gctcttcgtc gtaaagtccg tggcttagcg
     3061 gaaggactgg gcttgactga aatcatttcg tatgccttga caacaccaga gaaagctgtt
     3121 gagtttgcag tggcaccaag tcacctaaca gaactcatgt ggccaatgtc tgtggaacgt
     3181 tcagcgcttc gtcaaaatat ggtttcaggg atgttagata cggtagctta taatgtggct
     3241 cgtaaacaaa gtaacttggc tctttatgaa attggaaaaa tctttgaaca agaagctaat
     3301 ccaaaagaag accttcctaa tgaagtgaac cattttgctt ttgctatttg tggcttagtg
     3361 gcacaaaaag atttccaaac ccaagcacag gtagtggatt tttaccatgc taaaggtatc
     3421 ttggatactt tatttgcaaa ccttaacctc aaggttcaat atgtgccaac caaagaccta
     3481 gcaagcatgc acccaggacg gacagcgctt atcctattag atgagcaagt gattggattt
     3541 gttggtcaag ttcatccagg aacagcaaag gcttatagta ttccagaaac ctatgtggca
     3601 gaattggata tggcggcttt agaagcagcg ttgtcaagtg atcaaacttt tgtggagatt
     3661 accaaattcc ctgctatgac acgtgatgtt gccttgttac ttgaccgtga agtgagtcat
     3721 caggccattg taacagctat tgaatcagca ggtgtaaaac gcttgactaa gattaagctc
     3781 tttgacgtgt atgaaggagt aactattcag gctggcaaaa aatcaatggc ttatagcctt
     3841 actttccaaa atccaaatga caatttaacg gatgaagaag ttgctaaata catggagaaa
//
LOCUS       DAUCLR010000116         3821 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.118, whole genome shotgun sequence.
ACCESSION   DAUCLR010000116 DAUCLR010000000
VERSION     DAUCLR010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.118"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            97..1017
                     /gene="lmb"
                     /locus_tag="VGT87_001702"
     CDS             97..1017
                     /gene="lmb"
                     /locus_tag="VGT87_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/laminin-binding adhesin Lmb"
                     /protein_id="HEQ3814529.1"
                     /translation="MKKGFFLMAMVVSLVMIAGCDKSANPKQPTQGMSVVTSFYPMYA
                     MTKEVSGDLNDVRMIQSGAGIHSFEPSVNDVAAIYDADLFVYHSHTLEAWARDLDPNL
                     KKSKVDVFEASKPLTLDRVKGLEDMEVTQGIDPATLYDPHTWTDPVLAGEEAVNIAKE
                     LGRLDPKHKDSYTKKAKAFKKEAEQLTEEYTQKFKKVRSKTFVTQHTAFSYLAKRFGL
                     KQLGISGISPEQEPSPRQLKEIQDFVKEYNVKTIFAEDNVNPKIAHAIAKSTGAKVKT
                     LSPLEAAPSGNKTYLENLRANLEVLYQQLK"
     gene            1030..3501
                     /locus_tag="VGT87_001703"
     CDS             1030..3501
                     /locus_tag="VGT87_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEQ3814530.1"
                     /translation="MKKTYGYIGSVAAILLATHIGSYQLGKHHMGLATKDNQIAYIDD
                     SKGKAKAPKTNKTMDQISAEEGISAEQIVVKITDQGYVTSHGDHYHFYNGKVPYDAII
                     SEELLMTDPNYHFKQSDVINEILDGYVIKVNGNYYVYLKPGSKRKNIRTKQQIAEQVA
                     KGTKEAKEKGLAQVAHLSKEEVAAVNEAKRQGRYTTDDGYIFSPTDIIDDLGDAYLVP
                     HGNHYHYIPKKDLSPSELAAAQAYWSQKQGRGARSSDYRPTPAPGRRKAPIPDVTPNP
                     GQGHQPDNGGYHPAPPRPNDASQNKHQRDEFKGKTFKELLDQLHRLDLKYRHVEEDGL
                     IFEPTQVIKSNAFGYVVPHGDHYHIIPRSQLSPLEMELADRYLAGQTEDNDSGSEHSK
                     PSDKEVTHTFLGHRIKAYGKGLDGKPYDTSDAYVFSKESIHSVDKSGVTAKHGDHFHY
                     IGFGELEQYELDEVANWVKAKGQADELAAALDQEQGKEKPLFDTKKVSRKVTKDGKVG
                     YMMPKDGKDYFYARDQLDLTQIAFAEQELMLKDKKHYRYDIVDTGIEPRLAVDVSSLP
                     MHAGNATYDTGSSFVIPHIDHIHVVPYSWLTRDQIATIKYVMQHPEVRPDVWSKPGHE
                     ESGSVIPNVTPFDKRAGMPNWQIIHSAEEVQKALAEGRFATPDGYIFDPRDVLAKETF
                     VWKDGSFSIPRADGSSLRTINKSDLSQAEWQQAQELLAKKNAGDATDTDKPKEKQQAD
                     KRNENQQPSEASKEEEKESDDFIDSLPDYGLDRATLEDHINQLAQKANIDPKYLIFQP
                     EGVQFYNKNGELVTYDIKTLQQINP"
     gene            3670..>3821
                     /locus_tag="VGT87_001704"
     CDS             3670..>3821
                     /locus_tag="VGT87_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEQ3814531.1"
                     /translation="MSEEKLKSKIEQASGGLKEGAGKLTGDKELEAKGFVEKTIAKGK
                     ELADDA"
BASE COUNT         1329 a          706 c          810 g          976 t
ORIGIN      
        1 ggcgtgtaaa agatggtagt tgccattgac aaagcattga taaaggagta aaattaactg
       61 gttaataact ggttaaatta taattgagga ggtactatga aaaaaggttt ttttctcatg
      121 gctatggtcg tgagtttagt aatgatagca gggtgtgata agtcagcaaa ccccaaacag
      181 cctacgcaag gcatgtcagt tgtaaccagc ttttacccaa tgtatgcgat gacaaaagaa
      241 gtatctggag acctaaatga tgtgaggatg atccaatcag gtgcaggcat tcattccttt
      301 gaaccgtctg taaatgatgt ggcagctatt tatgacgcgg atttgtttgt ttaccattca
      361 catactttag aagcttgggc aagggatcta gaccctaatt taaaaaaatc aaaggttgat
      421 gtgtttgaag cgtcaaaacc tctgacacta gatagagtca aagggctaga agatatggaa
      481 gtcacacaag gcattgaccc tgcgacactt tatgacccac atacctggac agatcccgtt
      541 ttagctggtg aggaagctgt taatatcgct aaagagctag gacgtttgga tcctaaacac
      601 aaagacagtt acactaaaaa ggctaaggct ttcaaaaaag aagcagagca actaactgaa
      661 gaatacactc aaaaatttaa aaaggtgcgc tcaaaaacat tcgtgacgca gcacacggca
      721 ttttcttatc tggctaaacg attcggcttg aaacaacttg gtatctcggg tatttctcca
      781 gagcaagagc cctctcctcg ccaattgaaa gaaattcaag actttgtcaa agaatacaac
      841 gtcaagacta tttttgcaga agacaatgtc aatcccaaaa ttgctcatgc tattgcgaaa
      901 tcaacaggag ctaaagtaaa gacattaagt ccacttgaag ctgctccaag cggaaacaag
      961 acatatctag aaaatcttag agcaaatttg gaagtgctct atcaacagtt gaagtaaagg
     1021 agattattag tgaagaaaac atatggttat atcggctcag ttgctgctat tttactagct
     1081 actcatattg gaagttacca gcttggtaag catcatatgg gtctagcaac aaaggacaat
     1141 cagattgcct atattgatga tagcaaaggt aaggcaaaag cccctaaaac aaacaaaacg
     1201 atggatcaaa tcagtgctga agaaggcatc tctgctgaac agatcgtagt caaaattact
     1261 gaccaaggtt atgtgacctc acacggtgac cattatcatt tttacaatgg gaaagttcct
     1321 tatgatgcga ttattagtga agagttgttg atgacggatc ctaattacca ttttaaacaa
     1381 tcagacgtta tcaatgaaat cttagacggt tacgttatta aagtcaatgg caactattat
     1441 gtttacctca agccaggtag taagcgcaaa aacattcgaa ccaaacaaca aattgctgag
     1501 caagtagcca aaggaactaa agaagctaaa gaaaaaggtt tagctcaagt ggcccatctc
     1561 agtaaagaag aagttgcggc agtcaatgaa gcaaaaagac aaggacgcta tactacagac
     1621 gatggctata tttttagtcc gacagatatc attgatgatt taggagatgc ttatttagta
     1681 cctcatggta atcactatca ttatattcct aaaaaagatt tgtctccaag tgagctagct
     1741 gctgcacaag cctactggag tcaaaaacaa ggtcgaggtg ctagatcgtc tgattaccgc
     1801 ccgacaccag ccccaggtcg taggaaagcc ccaattcctg atgtgacgcc taaccctgga
     1861 caaggccatc agccagataa cggtggctat catccagcgc ctcctaggcc aaatgatgcg
     1921 tcacaaaaca aacaccaaag agatgagttt aaaggaaaaa cctttaagga acttttagat
     1981 caactacacc gtcttgattt gaaataccgt catgtggaag aagatgggtt gatttttgaa
     2041 ccgactcaag tgatcaaatc aaacgctttt gggtatgtgg tgcctcatgg agatcattat
     2101 catattatcc caagaagtca gttatcacct cttgaaatgg aattagcaga tcggtactta
     2161 gccggccaaa ctgaggacaa tgactcaggt tcagagcact caaaaccatc agataaagaa
     2221 gtgacacata cctttcttgg tcatcgcatc aaagcttacg gaaaaggctt agatggtaaa
     2281 ccatatgata cgagtgatgc ttatgttttt agtaaagaat ccattcattc agtggataaa
     2341 tcaggagtta cagctaaaca cggagatcat ttccactata taggatttgg agaacttgaa
     2401 caatatgagt tggatgaggt cgctaactgg gtgaaagcaa aaggtcaagc tgatgagctt
     2461 gctgctgctt tggatcagga acaaggcaaa gaaaaaccac tctttgacac taaaaaagtg
     2521 agtcgcaaag taacaaaaga tggtaaagtg ggctatatga tgccaaaaga tggcaaggac
     2581 tatttctatg ctcgtgatca acttgatttg actcagattg cctttgccga acaagaacta
     2641 atgcttaaag ataagaagca ttaccgttat gacattgttg acacaggtat tgagccacga
     2701 cttgctgtag atgtgtcaag tctgccgatg catgctggta atgctactta cgatactgga
     2761 agttcgtttg ttatccctca tattgatcat atccatgtcg ttccgtattc atggttgacg
     2821 cgcgatcaga ttgcaacaat caagtatgtg atgcaacacc ccgaagttcg tccggatgta
     2881 tggtctaagc cagggcatga agagtcaggt tcggtcattc caaatgttac gccttttgat
     2941 aaacgtgctg gtatgccaaa ctggcaaatt atccattctg ctgaagaagt tcaaaaagcc
     3001 ctagcagaag gtcgttttgc aacaccagac ggctatattt tcgatccacg agatgttttg
     3061 gccaaagaaa cttttgtatg gaaagatggc tcctttagca tcccaagagc agatggcagt
     3121 tcattgagaa ccattaataa atctgatcta tcccaagctg agtggcaaca agctcaagag
     3181 ttattggcaa agaaaaacgc tggtgatgct actgatacgg ataaacccaa agaaaagcaa
     3241 caggcagata agcgcaatga aaaccaacag ccaagtgaag ccagtaaaga agaagaaaaa
     3301 gaatcagatg actttataga cagtttacca gactatggtc tagatagagc aaccctagaa
     3361 gatcatatca atcaattagc acaaaaagct aatatcgatc ctaagtatct cattttccaa
     3421 ccagaaggtg tccaatttta taataaaaat ggtgaattgg taacttatga tatcaagaca
     3481 cttcaacaaa taaaccctta accaaaagaa gatctcatta ttaaagcact gctttatcaa
     3541 agcaagttac ggtgattttg aagtcatttt atgtaacgag tagtgataaa agttggataa
     3601 tagcggtttt cttttgcaaa gaaatggtat ccatgttaga ataataaaaa aaagaggagg
     3661 attctaatca tgtcagaaga aaaattaaaa tcaaaaattg agcaagcatc tggtggccta
     3721 aaagaaggtg ctggtaagtt gacaggtgat aaagagctag aagcaaaagg atttgttgaa
     3781 aaaacaattg ctaaaggcaa agaacttgca gacgatgcaa a
//
LOCUS       DAUCLR010000117         3687 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.119, whole genome shotgun sequence.
ACCESSION   DAUCLR010000117 DAUCLR010000000
VERSION     DAUCLR010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.119"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            167..1018
                     /locus_tag="VGT87_001705"
     CDS             167..1018
                     /locus_tag="VGT87_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEQ3814532.1"
                     /translation="MKGRIVITIRDLASLPIFVYGDGNKKVPGALSPFVWTEVFLKTI
                     NQEPNQLILHIWPMTRTVILGMLDRQLPYFELAKTEIGNNGYVPVTRNIGGLAVVADD
                     GILNFSLVIPDHFSESISISNAYLIMVDVIRESFSDYYQRIEYHEIKNSYCPGNFDLS
                     IAGRKFAGIAQRRIKKGIVVSIYLSVCGDQAARGQLIKDFYEAGTQGEVTKVNYPQID
                     PECMATLSELLETPFTVAEVLERLRLTLRQLGFSLTEKSPDQALLTNFDAVYERMQLE
                     VVRKEGK"
     gene            1015..2214
                     /locus_tag="VGT87_001706"
     CDS             1015..2214
                     /locus_tag="VGT87_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEQ3814533.1"
                     /translation="MKERFSPLFEPLTLPNGSQLDNRFVLSPMVTNSSTKDGYVTQDD
                     VSYALRRAASAPLQITGAAYVDPYGQLFEYGFSVTKDADISGLKELAQAMKAKGAKAV
                     LQLTHAGRFASHALTKYGFVYGPSYMQLRSPQPHEVKPLTGQQIEELIAAYAQATRRA
                     IQAGFDGVEVSSAQRLLIQTFFSTFSNKRTDSYGCQTLFNRSKLTLAVLQAVQQVIKQ
                     EAPDGFIFGFRATPEETRGNDIGYSIDEFLQLMDWVLNVAKLDYLAIASWGRHVFRNT
                     VRSPGPYYGRRVNQVVRDYLRNKLPVMATGGMNTPDKAIEALAHADFIGVSTPFVVDP
                     EFAHKIKEGCEESIHLRIRPADLKSLAIPQASFKDIVPLMDYGESLPKESRTLFRSLT
                     HNYKEIK"
     gene            2211..3209
                     /locus_tag="VGT87_001707"
     CDS             2211..3209
                     /locus_tag="VGT87_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEQ3814534.1"
                     /translation="MKVSILDYGVIDKEKTPQEALLETRCLAQVADKLGFHRFWVAEH
                     HNIYAFAISSPELLMMHLADHTKQIRIGSGGIMPLHYSSFKIAEWIMTLEALHPNRID
                     LGIGNSLGTTLVQRALSSIHCKDSYSQVVTELYQYLNPDHLSPLPIFVNPRGNTYPQI
                     WTLSNSLETAELAGQLGLGYTFGIFPYIPKDPITEAKRVSAHYRKAFRPSKLLKIPKL
                     ILAVFIVLSDTDEEAEALAKPLDIWMLGQQDFNEFKTYPDVEEARNYHLTEKQREAIA
                     ANRSRMVIGSPHTVKKQLDRLIEACQADELLAIPLVPEFANRQRTLELLADLYVTI"
     gene            3251..3583
                     /locus_tag="VGT87_001708"
     CDS             3251..3583
                     /locus_tag="VGT87_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEQ3814535.1"
                     /translation="MKKIANYLLIEKTDDRYTISMTPELQDDIGTIGYAEFTDNDHLA
                     VDDIILNLEASKTVMSVLSPLAGAVVERNEAATLTPTLLNSEKAEENWIVVLTDVDQA
                     AFDALEDA"
     gene            3576..>3687
                     /locus_tag="VGT87_001709"
     CDS             3576..>3687
                     /locus_tag="VGT87_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HEQ3814536.1"
                     /translation="MPSSFDLLGEMIGLLQTEQLTSSWACPLPNALTKRQD"
BASE COUNT         1110 a          663 c          799 g         1115 t
ORIGIN      
        1 ggagctgata gtaaacggaa gataatgacg ccagacattg tcttgcgttt gtattaatat
       61 ggaatagtaa tagatttcta gtaattaaaa cgaagttctg gaaagcaaga cactatagta
      121 gctaaaatct aaattttaag gtaaaataga gcaagcggtt tcaagtgtga aaggaagaat
      181 tgtgataact attagagatt tagcgtcact gccgattttt gtttacggtg atggaaataa
      241 aaaagttcca ggagctttga gtccttttgt gtggacagag gtgtttttaa aaactatcaa
      301 tcaagaacct aatcaactga tcttacatat ttggccgatg acaagaacag ttatattagg
      361 aatgttagat agacagcttc cctactttga gctggctaaa acagagattg gcaataatgg
      421 ttatgttcct gttactcgta atattggcgg tttggctgtt gtcgctgatg atgggatttt
      481 gaatttttcg cttgttattc cagaccattt ttcggaatct atcagtatta gtaatgctta
      541 tttaataatg gttgatgtca ttagagagag tttttcggat tattatcagc gtattgaata
      601 tcacgaaatt aaaaattctt attgccctgg taattttgat cttagtattg caggccgcaa
      661 gtttgcaggt atcgcccagc gtcgtatcaa aaagggaatt gttgtgtcta tttacttgag
      721 cgtttgtggg gatcaagcag cgcgtggtca actcatcaag gatttttatg aggccggaac
      781 acaaggagaa gttaccaagg ttaattaccc tcaaattgat ccagaatgca tggcaactct
      841 ttcagaactc cttgagacgc cttttacagt ggcagaagtc cttgaacgtc ttcgattgac
      901 cttgcgacag cttggctttt ccctcacgga aaaaagtcca gatcaagcct tactgactaa
      961 ttttgatgct gtctatgaac ggatgcagct agaagtcgtc agaaaggaag gtaaatgaaa
     1021 gaaagattta gtcctttatt tgagcctttg actttgccaa atgggagtca attggataat
     1081 cgctttgttt tgtctcctat ggtgaccaac tcatccacaa aagacggtta tgtcacccaa
     1141 gatgatgtat cctatgcttt gagacgagca gcttctgccc ctctacaaat tacaggagcg
     1201 gcctatgtgg atccttatgg tcaattgttt gaatatggtt ttagtgttac aaaagatgcc
     1261 gatatttctg gcttgaaaga gctagctcaa gcgatgaagg caaagggagc taaagctgtc
     1321 ttacaactga cccacgctgg ccggtttgct agccatgcct tgactaaata tggctttgtc
     1381 tatgggccta gttacatgca gttgagatct ccccaacctc atgaagtcaa acctttaaca
     1441 ggtcagcaaa tagaggaatt gattgcagct tacgctcagg ccactcggcg ggcaattcaa
     1501 gcaggctttg atggcgtgga agtatcttcg gcgcagcgct tattgattca aacgttcttt
     1561 tcgacttttt cgaacaagag gacagatagt tatggttgtc aaacactttt taatcgctct
     1621 aagttaactc tagctgtttt gcaagctgtt caacaggtga ttaaacaaga agctcctgat
     1681 gggtttattt ttggatttcg tgctacacca gaagaaacta ggggaaatga tattggttat
     1741 agtattgacg agtttttgca gttgatggat tgggtactta atgttgcaaa acttgattat
     1801 ttagcgatag caagttgggg ccgtcatgta tttcgaaata ctgttcgttc tcctggccct
     1861 tattatggaa ggcgagttaa tcaggtagtt agagattatt taagaaataa gctacctgtt
     1921 atggctacag ggggaatgaa cacacctgac aaagctattg aagcattagc tcatgctgat
     1981 tttattggag tatctacacc ttttgtcgtg gatccagaat ttgctcataa aatcaaagaa
     2041 gggtgtgaag agagtattca tttaaggatt cgacctgctg atttgaaaag tttagcgatt
     2101 cctcaggctt ctttcaaaga tattgtacct ttgatggact acggggaatc attgcctaaa
     2161 gaatcaagaa ctttatttcg tagtcttact cacaattata aggagattaa atgaaagtca
     2221 gtattcttga ttatggggtg attgataaag aaaaaacacc ccaagaagct ttattagaaa
     2281 cgcgctgctt agcacaagta gccgacaaac ttggttttca tcgtttttgg gtagcagagc
     2341 atcataatat ttatgccttt gcaataagca gtccagagct gttgatgatg catttagcag
     2401 atcataccaa acagattcgg ataggttcag gaggtattat gcctctacat tatagtagct
     2461 ttaagattgc agaatggata atgactttag aggccttgca ccctaatcgg attgatttag
     2521 gtattggcaa ctctttagga actacgttag ttcaaagagc tctatcaagt atccattgca
     2581 aagacagcta tagtcaggtt gtcacagaat tataccagta tcttaatcct gatcatttgt
     2641 caccactacc gatttttgtt aaccctaggg gtaatactta ccctcagatt tggacgctaa
     2701 gcaatagttt ggagacagca gaactggctg gtcaattagg tttaggctat acctttggta
     2761 ttttccccta tataccaaaa gatcctataa cagaagctaa aagagtatcg gcacattata
     2821 gaaaggcttt taggccttca aagctattaa aaatccccaa gttaatatta gcagttttta
     2881 ttgtcctttc tgataccgac gaggaagcag aagctttggc taaaccgttg gacatttgga
     2941 tgcttggtca gcaagatttt aatgaattta agacctaccc tgatgttgag gaggctagaa
     3001 actatcactt aacagagaaa caaagagaag ccattgcagc aaatcgtagt cgcatggtta
     3061 ttggatcacc tcacacagtc aagaaacaat tggataggct gatagaagct tgtcaagctg
     3121 acgaattatt agccatcccc ttagttcctg aatttgccaa tagacaacga accttagagc
     3181 tgctagcaga cttatacgtg accatttaac cttaccaaaa acaattccct aaactgaaag
     3241 gaaagaaaca atgaaaaaaa ttgcaaatta cttattaatt gagaaaacag atgaccgcta
     3301 taccattagc atgacaccag aattacaaga cgatattggt actattggtt acgctgaatt
     3361 tacggataac gaccaccttg ccgttgatga cattatctta aatctagagg cttctaagac
     3421 cgtcatgtct gtcttgtcac cattggctgg ggcagttgtt gagcgcaatg aggcagccac
     3481 actaacacca acccttttga attcagaaaa agctgaggaa aattggattg ttgtgctaac
     3541 agatgttgat caagcagctt ttgatgcctt agaagatgcc tagttcattt gatttattag
     3601 gggaaatgat tggtctgttg caaacagagc agcttactag ttcttgggcc tgtcccttgc
     3661 ctaatgcttt aacaaagcga caagact
//
LOCUS       DAUCLR010000118         3557 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.120, whole genome shotgun sequence.
ACCESSION   DAUCLR010000118 DAUCLR010000000
VERSION     DAUCLR010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.120"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(296..688)
                     /locus_tag="VGT87_001710"
     CDS             complement(296..688)
                     /locus_tag="VGT87_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEQ3814537.1"
                     /translation="MCLSYNRRNQRKKVVLMTIKKFSLLAIASLSLLSLAACDMDDKD
                     DHMDNQPKTSQTSKKVKLSEDKAKSIALKDASVTEADAQMLSVTQDNEDGKAVYEIEF
                     QNKDQEYSYTIDANSGDIVEKSSEPIND"
     gene            complement(815..1072)
                     /locus_tag="VGT87_001711"
     CDS             complement(815..1072)
                     /locus_tag="VGT87_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEQ3814538.1"
                     /translation="MDPKKIARINELAKKKKTVGLTGPEKVEQAKLREEYIEGYRRSV
                     RHHIEGIKLVDEEGNDVTPEKLRQVQREKGLHGRSLDDPKS"
     gene            complement(1226..3265)
                     /locus_tag="VGT87_001712"
     CDS             complement(1226..3265)
                     /locus_tag="VGT87_001712"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEQ3814539.1"
                     /translation="MSKNLLIELGLEELPAYVVTPSEKQLGERLATFLTENHLSFEDI
                     QTFSTPRRLAVRVSGLAAQQTDLTEDFKGPAKKIALDADGNFSKAAQGFVRGKGLTTD
                     AIEFREVKGEEYVYVTKHEAGKPAKEVLLGVTEVLSAMTFPVSMHWANNSFEYIRPVH
                     TLTVLLNDEALELDFLDIHSGRVSRGHRFLGTETTITSADSYEADLRSQFVIADAKER
                     QEMIVEQIKTLEVEQGVQVDIDEDLLNEVLNLVEFPTAFMGSFEAKYLDVPEEVLITS
                     MKNHQRYFVVRDQAGHLMPNFVSVRNGNDQAIENVIKGNEKVLVARLEDGEFFWREDQ
                     KLQIADLVAKLTNVTFHEKIGSLAEHMDRTRVIAASLAKEANLSAEEVTAVDRAAQIY
                     KFDLLTGMVGEFDELQGIMGEKYALLAGEDAAVARAIREHYLPDAAGGALPETKVGAV
                     LALADKLDTLLSFFSVGLIPSGSNDPYALRRATQGIVRILDHFGWRIPMDKLVDSLYD
                     LSFDSLTYANKADVMNFIRARVDKMMGKATPKDIREAVLESSTFVVPEMLAAAEALVK
                     ASHTENYKPAVESLSRAFNLAEKADASVQVDPSLFENEQENTLFAAIQGLTLAGSAAQ
                     QLEQVFALSPVINDFFDNTMVMAEDQALKNNRVAILSDLVSKAKTIAAFNQLNTK"
BASE COUNT         1016 a          802 c          680 g         1059 t
ORIGIN      
        1 tatattatca agaattttta aagctaaaaa aaagctgata agataggtag cttatcccac
       61 tagatgatct tgaaaaatcc actgatgaca accacgtctc ctcatttcta acgaggtaat
      121 ctttcttatc aaagaaaatc aaaagaccca acaatcactc tgctaatcca gagtcatcat
      181 tgggtcataa agagaactaa gcctcaaagg caacacggta tcaaacagcc gtgcctaaga
      241 ggcaaagaca attggacaca agagttaagg ctgtcttatt cattacagtt cttgattagt
      301 cattgattgg ttcagaagat ttttccacaa tatcccctga gttggcgtca atggtatagc
      361 tgtattcttg atctttgttt tgaaactcga tttcatacac ggctttgcca tcttcattat
      421 cttgtgtaac tgatagcatt tgagcatcag cttccgtaac agaagcatct tttaacgcaa
      481 ttgatttggc tttgtcttct gaaagtttaa ctttcttgct agtttgactt gtctttggtt
      541 gattatccat atgatcgtct ttatcatcca tatcacaggc tgcaagactg agtaaagaga
      601 ggctggctat ggctagtaga gagaattttt taattgtcat aagtactacc ttctttcttt
      661 gatttcttct attataactc aaacacatgg tttttcccaa tattttgact atacaatata
      721 aaaacgtgtg gtcacttcga agtatcatac catttaaaat aaaagctaac agtcatcacg
      781 ataaacaaaa agcagttcaa agctgctttt tgtcttatga ttttgggtca tctaatgaac
      841 ggccgtgcaa gcctttttca cgttgcacct gtcttaattt ttcaggggtc acgtcgtttc
      901 cttcttcgtc caccaatttg atgccttcga tatggtgtct gacagagcga cgatagcctt
      961 caatgtattc ttcacggagt tttgcctgtt ccactttttc aggtccagtc aagcctaccg
     1021 tttttttctt tttagccagc tcattgatac gagcaatttt tttaggatcc atgttaagcc
     1081 actaagagcc tgggaaaaaa gtgtaagact taaaaaactg acttgactct cactgtgaaa
     1141 agtaatgttt aaaggccttg ttctttccac tcgggtactg aactcacttt tctagctaag
     1201 tccccaacgg ctctttcctt tctatttact tggtgtttag ttggttaaaa gcagcaatgg
     1261 tcttagcttt gcttactaaa tctgataaaa tagctacacg gttgttctta agggcttgat
     1321 cttctgccat aaccattgta ttatcaaaga aatcattaat cactgggcta agggcaaaga
     1381 cctgttctaa ctgctgggca gcactgcctg ctagagtcaa gccttgaata gctgcaaaca
     1441 aagtgttttc ttgctcattt tcaaagaggc tagggtccac ctgaacagaa gcgtccgctt
     1501 tttctgccaa attaaaagca cgtgacaaag attcaactgc tggtttgtaa ttttccgtat
     1561 ggctggcttt gacaagtgct tcagctgccg ctaacatttc aggaaccaca aaggttgaac
     1621 tctctaaaac tgcttcacga atatcttttg gtgtggcttt gcccatcatc ttatccacac
     1681 gagcacggat aaagttcatc acatccgcct tattggcata ggtcaaacta tcaaaggaca
     1741 agtcatataa gctatctacc agcttatcca ttgggatgcg ccatccaaaa tgatcaagaa
     1801 tacgaacgat accctgcgtt gcccgacgaa gcgcataagg atcattagag ccagatggaa
     1861 tcaagccaac agaaaagaat gacaagagag tatccaattt atctgccaag gctaggacag
     1921 cgccaacttt ggtttcaggt agcgcacctc ctgcagcatc aggtaagtag tgttctcgga
     1981 tagcccttgc cacagcagca tcttcaccag ctagaagagc atatttttca cccataatcc
     2041 cttgcagctc atcaaattca ccaaccatac ctgttaaaag gtcaaatttg taaatttggg
     2101 cagcacggtc aacagctgtg acttcctcag cagataggtt ggcttcctta gcaagtgatg
     2161 cggcaatgac acgcgtacga tccatgtgtt cagcaagaga gccgattttt tcatggaagg
     2221 tcacattggt caatttggca accaaatcag caatttgcaa tttctgatcc tcacgccaga
     2281 agaattcacc atcttctaag cgagctacca aaactttttc gttcccttta ataacatttt
     2341 caatggcttg atcattccca ttacgaactg atacaaagtt tggcatcaag tgaccagcct
     2401 gatcacgtac tacaaaataa cgttggtggt ttttcattga tgttatcaat acttcttctg
     2461 ggacatcaag gtatttagcc tcaaaacttc ccatgaaagc tgttggaaac tcgaccaagt
     2521 tcaaaacttc attaagcaag tcttcgtcaa tgtcaacttg cacaccctgc tccacttcca
     2581 gcgtcttgat ttgctccaca atcatctctt gacgctcttt agcatcagcg ataacaaact
     2641 ggctgcgcaa atcagcctca taagaatcag cacttgtgat agtggtttca gtacctaaaa
     2701 aacggtgacc acggctgacg cgtccagaat gaatatccaa gaagtcaagt tccaaggcct
     2761 catcattcaa cagaactgtc aaggtgtgaa caggacgaat gtattcaaaa ctattgttag
     2821 cccagtgcat gctgactggg aaggtcattg ctgacaacac ttcagtaacg cctagaagca
     2881 cttccttagc aggttttcca gcttcgtgtt tggtcacata aacgtattct tctcctttga
     2941 cttcgcggaa ttcgatagca tctgtggtca agcctttgcc acgaacaaat ccttgagcag
     3001 cttttgagaa attcccatca gcatcaaggg caattttctt agcaggccct ttaaagtcct
     3061 cagtcaaatc cgtttgctga gcagctaacc cagacacacg cactgccaaa cggcgtggag
     3121 ttgaaaaggt ttgtatatct tcaaaagaga gatgattttc tgttaagaaa gtcgccaagc
     3181 gctctcccag ttgtttttca cttggtgtga ccacgtaggc tggaagttct tccagaccaa
     3241 gttcgattag tagattttta ctcataatta agataccaag ggcgttaagc ggacaccatc
     3301 aaaatagcaa agcgacgcag gtgcttgcac ctaggaagtc ttggtctttt tgacatagtc
     3361 cgtagcccgt gctcagttcc tttctctaaa aagtatcttt ttgattgttc ccttaggtag
     3421 ggggacgcaa ggtgaaatta tccaatggat aatttcagaa aacaaaagtg ttccattcct
     3481 agttagccag cctaaagtct cgccaactcc ctcgttcgat aagtaacctt gccgaattgt
     3541 cactacagcg ggagtaa
//
LOCUS       DAUCLR010000119         3462 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.121, whole genome shotgun sequence.
ACCESSION   DAUCLR010000119 DAUCLR010000000
VERSION     DAUCLR010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.121"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            359..>3462
                     /gene="scpA/B"
                     /locus_tag="VGT87_001713"
     CDS             359..>3462
                     /gene="scpA/B"
                     /locus_tag="VGT87_001713"
                     /EC_number="3.4.21.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021340616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C5a peptidase ScpA/B"
                     /protein_id="HEQ3814540.1"
                     /translation="MRKKQKLPFDKLAIALMSTSILLNAQSDIKANTVTEDTPATEQA
                     VETPQPTAVSEEAPSSSKETNPPQIPDDAEETVADKANDPAPQAPAKTADTPATSKAT
                     IRDLNDPSQVKTLQEKAGKGAGTVVAVIDAGFDKNHEAWRLTDKTKARYQSKEDLEKA
                     KKEHGITYGEWVNDKVAYYHDYSKDGKTAVDQEHGTHVSGILSGNAPSETKEPYRLEG
                     AMPEAQLLLMRVEIVNGLADYARNYAQAIRDAVNLGAKVINMSFGNAALAYANLPDET
                     KKAFDYAKSKGVSIVTSAGNDSSFGGKTRLPLADHPDYGVVGTPAAADSTLTVASYSP
                     DKQLTETATVKTDDHQAKEMPVLSTNRFEPNKAYDYAYANRGTKEDDFKDVKGKIALI
                     ERGDIDFKDKIANAKKAGAVGVLIYDNQDKGFPIELPNVDQMPAAFISRKDGLLLKDN
                     PQKTITFNATPKVLPTASGTKLSRFSSWGLTADGNIKPDIAAPGQDILSSVANNKYAK
                     LSGTSMSAPLVAGIMGLLQKQYETQYPDMTPSERLDLAKKVLMSSATALYDEDEKAYF
                     SPRQQGAGAVDAKKASAATMYVTDKDNTSSKVHLNNVSDKFEVTVTVHNKSDKPQELY
                     YQATVQTDKVDGKHFALAPKALYETSWQKITIPANSSKQVTVPIDASRFSKDLLAQMK
                     NGYFLEGFVRFKQDPKKEELMSIPYIGFRGDFGNLSALEKPIYDSKDGSSYYHEANSD
                     AKDQLDGDGLQFYALKNNFTALTTESNPWTIIKAVKEGVENIEDIESSEITETIFAGT
                     FAKQNDDSHYYIHRHANGKPYAAISPNGDGNRDYVQFQGTFLRNAKNLVAEVLDKEGN
                     VVWTSEVTEQVVKNYNNDLASTLGSTRFEKTRWDGKDKDGKVVANGTYTYRVRYTPIS
                     SGAKEQHTDFDVIVDNTTPEVATSATFSTEDRRLTLASKPKTSQPVYRERIAYTYMDE
                     DLPTTEYISPNEDGTFTLPEEAETMEGATVPLKMSDFTYVVEDMAGNITYTPVTKLLE
                     GHSN"
BASE COUNT         1174 a          725 c          710 g          853 t
ORIGIN      
        1 ctaaaacgca aagaagaaaa ctaagccttt aaaacttggt ttttgtaacg atgcaataga
       61 caaaagcaag caaggccaaa aactgagaaa gtcctaaaaa gctggccttt acccctcaaa
      121 attaatgttt tataataaag atgttagtaa tataattgat aaatgagata catttaatca
      181 ttatggcaaa agcaagaaaa atagctgtat catatgcaaa taacccctgt ttgctcttta
      241 aaaaagacgt tatccttatt tctccacgca cagatggaca gctaggagag aatcgtttga
      301 ttctctcttt tcttaatggt cataaagaca aagtctctca tcatgaaagg acgacacatt
      361 gcgtaaaaaa caaaaattac catttgataa acttgccatt gcgctcatgt ctacgagcat
      421 cttgctcaat gcacaatcag acattaaagc aaatactgtg acagaagaca ctcctgctac
      481 cgaacaagcc gtagaaactc cacaaccaac agcggtttct gaggaagcac catcatcatc
      541 aaaggaaact aaccctccac aaattcctga tgacgcagaa gaaacagtag cagataaggc
      601 taatgatcca gcccctcaag ctcctgctaa aactgctgat acaccagcaa cctcaaaagc
      661 gactattagg gatttgaacg acccttctca ggtcaaaacc ctgcaggaaa aagcaggcaa
      721 gggagctggg actgttgttg cagtgattga tgctggtttt gataaaaatc atgaagcgtg
      781 gcgtttaaca gacaaaacca aagcacgtta ccaatcaaaa gaagatcttg aaaaagctaa
      841 aaaagagcac ggtattacct acggcgagtg ggtcaatgat aaggttgctt attaccacga
      901 ctatagcaaa gatggtaaaa ccgctgtcga tcaagagcac ggcacacacg tgtcagggat
      961 cttgtcaggg aatgctccat ctgaaacgaa agaaccttac cgcctagaag gtgcgatgcc
     1021 tgaggctcaa ttgcttttga tgcgtgtcga aattgtaaat ggactagcag actatgctcg
     1081 taactacgct caagctatca gagatgctgt caacttggga gctaaggtga ttaatatgag
     1141 ctttggtaat gctgcactag cttacgccaa ccttccagac gaaaccaaaa aagcctttga
     1201 ctatgccaaa tcaaaaggtg ttagcattgt gacctcagct ggtaatgata gtagctttgg
     1261 gggcaagacc cgtctacctc tagcagatca tcctgattat ggggtggttg ggacacctgc
     1321 agcggcagac tcaacattga cagttgcttc ttacagccca gataaacagc tcactgaaac
     1381 tgctacggtc aaaacagacg atcatcaagc taaagaaatg cctgttcttt caacaaaccg
     1441 ttttgagcca aacaaggctt acgactatgc ttatgctaat cgtgggacga aagaggatga
     1501 ttttaaggat gtcaaaggca aaattgccct tattgaacgt ggcgatattg atttcaaaga
     1561 taagattgca aacgctaaaa aagctggtgc tgtaggggtc ttgatctatg acaatcaaga
     1621 caagggcttc ccgattgaat tgccaaatgt tgaccagatg cctgcggcct ttatcagtcg
     1681 aaaagatggt ctcttattaa aagacaatcc ccaaaaaacc atcaccttca atgcgacacc
     1741 taaggtattg ccaacagcaa gtggcaccaa actaagccgc ttctcgagct ggggtctgac
     1801 agctgacggc aatattaagc cagatattgc agcacccggc caagatattt tgtcatcagt
     1861 ggctaacaac aagtatgcca aactttctgg aactagtatg tctgcgccat tagtagcggg
     1921 tatcatggga ctgttgcaaa agcaatatga gacacagtat cctgatatga caccatcaga
     1981 gcgtcttgat ttagctaaaa aagtattgat gagctcagca actgccttat atgatgaaga
     2041 tgaaaaagct tatttttctc ctcgccaaca aggagcagga gcagtcgatg ctaaaaaagc
     2101 ttcagcagca acgatgtatg tgacagataa ggacaatacc tcaagcaagg ttcacctgaa
     2161 caatgtttct gataaatttg aagtaacagt aacagttcac aacaaatctg ataaacctca
     2221 agagttgtat taccaagcaa ctgttcaaac agataaagta gatggaaaac actttgcctt
     2281 ggctcctaaa gcattgtatg agacatcatg gcaaaaaatc acaattccag ccaatagcag
     2341 caaacaagtc accgttccaa tcgatgctag tcgatttagc aaggacttgc ttgcccaaat
     2401 gaaaaatggc tatttcttag aaggttttgt tcgtttcaaa caagatccta aaaaagaaga
     2461 gcttatgagc attccatata ttggtttccg aggtgatttt ggcaatctgt cagccttaga
     2521 aaaaccaatc tatgatagca aagacggtag cagctactat catgaagcaa atagtgatgc
     2581 caaagaccaa ttagatggtg atggattaca gttttacgct ctgaaaaaca actttacagc
     2641 acttactaca gagtctaacc catggacgat tattaaagct gtcaaagaag gagttgaaaa
     2701 catagaggat atcgaatctt cagagatcac agaaaccatt tttgcaggta cttttgcaaa
     2761 acaaaacgat gatagccact actatatcca ccgtcacgct aatggcaaac catatgctgc
     2821 gatctctcca aatggggacg gtaacagaga ttatgtccaa ttccaaggta ctttcttgcg
     2881 taatgctaaa aaccttgtgg ctgaagtctt ggacaaagaa ggaaatgttg tttggacaag
     2941 tgaggtaacc gagcaagttg ttaaaaacta caacaatgac ttggcaagca cacttggttc
     3001 aacccgtttt gaaaaaacgc gttgggacgg taaagataaa gacggcaaag ttgttgctaa
     3061 cggaacctac acctatcgtg ttcgctacac tccgattagc tcaggtgcaa aagaacaaca
     3121 tactgatttt gatgtgattg tagacaatac gacacctgaa gtcgcaacat cggcaacatt
     3181 ctcaacagaa gatcgtcgtt tgacacttgc atctaaacca aaaaccagcc aaccggttta
     3241 ccgtgagcgt attgcttaca cttatatgga tgaggatctg ccaacaacag agtatatttc
     3301 tccaaatgaa gatggtacct ttactcttcc tgaagaggct gaaacaatgg aaggcgctac
     3361 tgttccattg aaaatgtcag actttactta tgttgttgaa gatatggctg gtaacatcac
     3421 ttatacacca gtgactaagc tattggaggg ccactctaat aa
//
LOCUS       DAUCLR010000120         3456 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.122, whole genome shotgun sequence.
ACCESSION   DAUCLR010000120 DAUCLR010000000
VERSION     DAUCLR010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.122"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..973
                     /locus_tag="VGT87_001714"
     CDS             <1..973
                     /locus_tag="VGT87_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HEQ3814541.1"
                     /translation="LKELDKDYTAALSDAKQKSFVTQHAAFGYMALDYGLNQISINGV
                     TPDAEPSAKRIATLSKYVKKYGIKYIYFEENASSKVAKTLAKEAGVKAAVLSPLEGLT
                     EKEMKAGQDYFTVMRKNLETLRLTTDVAGKEILPEKDTTKTVYNGYFKDKEVKDRQLS
                     DWSGSWQSVYPYLQDGTLDQVWDYKAKKSKGKMTAAEYKDYYTTGYKTDVEQIKINGK
                     KKTMTFVRNGEKKTFTYTYAGKEILTYPKGNRGVRFMFEAKEPNAGEFKYVQFSDHAI
                     APEKAEHFHLYWGGDSQEKLHKELEHWPTYYGSDLSGREIAQEINAH"
     gene            1120..1842
                     /locus_tag="VGT87_001715"
     CDS             1120..1842
                     /locus_tag="VGT87_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEQ3814542.1"
                     /translation="MPKEQPLYLQIVDDLEVKIKKSMTENDKLLSERELSDLYGVSRI
                     TIRLALKELELRDLIYKKQGKGTYVSGIKEPVTDLSSTYSFTEQMKKMGKMPKTEILS
                     FEQYQVTPYLSGLLELDPDTEVFELERLRIADDMPLMFERSYIPAQPFQGLSIADLKR
                     KALYDIFAKDYQETIRLAEEEFYASIALDYEADLLGIKKGDPVLHIIRKTYNDKNILI
                     EFTFSIARADQFRYRVQHHPNG"
     gene            1862..3061
                     /locus_tag="VGT87_001716"
     CDS             1862..3061
                     /locus_tag="VGT87_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEQ3814543.1"
                     /translation="MFTKTQEALEALGAAITTKEIKQEPRLWQETMTFFEETRDSLDS
                     FLKRVCKSANGNNVHVIFTGAGTSEYIGNTICPYLKKVGNRQCYLFESVASTDLVAAP
                     DYYLAEEETVLLVSFARSGNSPESVAAVNLVNQLVPNSYHLTITCAKDGELAKKAQQD
                     ERSYLYLMPEDANDAGFAMTGSFTCMMLAALLIFDDANSIAQKEVYVTDMILAGQAVI
                     DQERRLQELADLGFERLVYLGSAGLAKLTQEAQLKMLELTAGQVATLYESSMGFRHGP
                     KSFINDRTLVIGFVNNDAYVRQYDLDMLEEIQADGIALKTLALLQGGDINFSEDQFRL
                     DTRHLLPEAYLAFPMILVAQTLALLTAVTLGNSPDTPSATGTVNRVVKGVTIHPYPTS
                     SRQCNKA"
     gene            complement(3158..3418)
                     /locus_tag="VGT87_001717"
     CDS             complement(3158..3418)
                     /locus_tag="VGT87_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEQ3814544.1"
                     /translation="MRKDIHPDYRPVVFLDTTTGYQFLSGSTKASKETVEFEGETYPL
                     IRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAK"
BASE COUNT         1096 a          632 c          732 g          996 t
ORIGIN      
        1 attaaaagag cttgacaaag actatacggc agcactttca gatgctaagc aaaagagctt
       61 tgtgacacaa cacgcagctt ttggttatat ggcacttgac tatggcttga accaaatttc
      121 tattaatggt gtcacaccag atgcagaacc atcagcaaaa cgtattgcta ctttgtcaaa
      181 atacgttaaa aaatatggca tcaaatacat ttattttgag gaaaatgcgt caagtaaagt
      241 cgcaaaaacc ctagctaaag aagcaggagt taaagcggct gtgcttagtc cgcttgaagg
      301 tttgactgaa aaagagatga aagctggcca agattacttt acggtcatgc gtaaaaacct
      361 tgaaacctta cgcttaacca ctgatgtggc tggtaaagaa attcttccag aaaaagacac
      421 gactaagaca gtttacaatg gttatttcaa agacaaagaa gtcaaagatc gtcaattatc
      481 tgactggtca ggtagctggc aatctgttta cccctatcta caagatggta ctttagacca
      541 agtttgggac tacaaggcta aaaaatctaa aggtaaaatg acagcagccg agtacaaaga
      601 ttactacact actggttata aaactgacgt ggaacaaatc aaaatcaatg gtaagaaaaa
      661 gaccatgacc tttgttcgta atggtgaaaa gaaaaccttc acttacacat acgccggcaa
      721 agaaatcttg acctatccaa aaggaaatcg tggagttcgt ttcatgtttg aagctaaaga
      781 accaaatgct ggcgaattca aatacgttca atttagtgac catgccattg ctcctgaaaa
      841 agcagagcat ttccacctgt actggggtgg tgacagccaa gaaaaattac ataaagagtt
      901 agaacattgg ccaacttact atggttcaga cttatctggt cgtgaaattg cccaagaaat
      961 caatgctcat taatcttaac tcactaagca aatcttcttt taaggaggtt tgctttttct
     1021 ttgtggagta aaaaaattaa taattctcaa cattaatttg acgggcactt atgattataa
     1081 tatactttaa gtggttataa caagttagag gtgaataaga tgccaaaaga acaaccactc
     1141 tatttacaga ttgttgatga cttggaagtg aaaatcaaaa aaagcatgac agaaaacgat
     1201 aagttgttgt cagaacgaga attaagtgac ctttacggtg ttagtcgtat cacgattaga
     1261 ctggctctta aggaattgga attacgagac ttaatttata aaaagcaggg caaggggact
     1321 tatgtttcag gaatcaaaga acctgtcact gatttatcaa gtacatatag ctttacagag
     1381 cagatgaaaa aaatgggaaa aatgcctaaa acagaaattc tttcctttga acagtatcag
     1441 gtaacacctt acttgtctgg tctcttagag cttgatcccg atacagaagt ttttgaatta
     1501 gaacgcctaa ggattgcaga tgatatgccc ttgatgtttg aacgatccta tattcctgct
     1561 cagccttttc aggggttaag cattgcggac ttgaagcgta aagctttgta cgatatcttt
     1621 gcaaaggact atcaagagac aatccgtctt gcagaagagg agttttatgc cagcattgcc
     1681 ttggactatg aagcggacct tttgggaatc aaaaaaggag atcctgtctt acatattatt
     1741 cgtaaaactt ataacgataa aaatattttg atcgaattta ctttcagtat tgcaagagca
     1801 gaccagttta ggtatagggt acaacatcac cccaatggtt aaagaaatgg gaggttaata
     1861 catgtttaca aaaacgcagg aagctttgga ggctcttggt gcagctatca ctaccaagga
     1921 aattaaacaa gaaccccgtt tatggcaaga aaccatgacg ttttttgaag aaacaagaga
     1981 ttctctggat tcttttttaa agagagtttg taaaagcgct aatggaaata acgttcatgt
     2041 catctttaca ggtgcgggta catctgagta catcggtaat accatttgcc cttacttgaa
     2101 gaaggtgggg aatcggcagt gctatctctt tgagagtgtt gcttcaaccg atttggttgc
     2161 ggcgccagat tattatcttg ctgaggaaga gacggtacta ttagtctcct ttgctaggag
     2221 tggtaatagt ccagaaagtg tagcggctgt taacctggtc aatcaactag tgccaaatag
     2281 ttatcattta accattacgt gtgccaaaga tggagaactg gcgaaaaaag cacagcaaga
     2341 tgagcgttct tacctttatt tgatgcctga agacgctaat gatgctggtt ttgccatgac
     2401 tggtagtttt acctgtatga tgctagcggc tcttttgatt tttgatgatg ctaacagtat
     2461 agctcagaaa gaagtttatg tgactgatat gattcttgct gggcaagctg ttattgacca
     2521 agaaagacgg ctacaagagc tagctgattt aggatttgaa cgtttagttt atctaggttc
     2581 tgctggttta gccaagttaa cccaagaagc tcaattaaaa atgttagaat tgacagctgg
     2641 acaagttgcg accctctacg agtcctctat gggctttagg catggtccga aatcttttat
     2701 taatgaccgt accttagtca ttggatttgt caataatgat gcttacgtca gacaatacga
     2761 tttggacatg ttagaggaaa tccaagcaga tggcattgcc cttaaaacct tggctctatt
     2821 gcaaggaggg gatatcaact tttcagagga ccaatttcga ctagacaccc gtcacttatt
     2881 gccagaagct tacttagctt ttcccatgat tttagtcgcg cagaccttgg ccctcttaac
     2941 ggccgttaca ttaggaaatt cccctgacac cccatctgcg acaggaacgg ttaatcgtgt
     3001 ggtcaaagga gtaacgattc acccttaccc gactagtagt cgtcaatgca acaaagctta
     3061 aagcttttgt gtagaagccg taaaacgaaa tgattttgtg ctagtaggcc aaaagaaaaa
     3121 ccctcctgat acaggaaggt tttttgtatt gtcttgctta ttttgctgcg tttgcgtctt
     3181 tgagaccgta ttttttgttg aagcggtcca cacgtccatc tgcttgagtg aacttttgac
     3241 gtcctgtgta gaatgggtgt gaatctgatg aaatttccac acggataagt gggtaagttt
     3301 caccttcaaa ctcaacagtt tctttagaag cttttgttga gccgctaagg aactggtaac
     3361 ctgtagttgt atcaaggaaa acaactggac gataatctgg atgaatgtct tttctcatct
     3421 taaaaaattc ctttctgcca tgacacttga tgcgtc
//
LOCUS       DAUCLR010000121         3354 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.123, whole genome shotgun sequence.
ACCESSION   DAUCLR010000121 DAUCLR010000000
VERSION     DAUCLR010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.123"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            113..604
                     /gene="purE"
                     /locus_tag="VGT87_001718"
     CDS             113..604
                     /gene="purE"
                     /locus_tag="VGT87_001718"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001875905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEQ3814545.1"
                     /translation="MKTPIISIIMGSKSDWATMQKTAEVLDNFGIAYEKKVVSAHRTP
                     DLMFKHAEEARGRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGLDSLYS
                     IVQMPGGVPVATMAIGEAGATNAALTALRILSIEDQNLADALAHFHEEQGKIAEESSG
                     ELI"
     gene            591..1664
                     /gene="purK"
                     /locus_tag="VGT87_001719"
     CDS             591..1664
                     /gene="purK"
                     /locus_tag="VGT87_001719"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEQ3814546.1"
                     /translation="MNSFKTIGIIGGGQLGQMMAIAAIYMGHKVITLDPTNDCPASRV
                     SEVIVAPYDDVEALGQLAARCDVLTYEFENVDADGLDAVVSACQLPQGTDLLRISQNR
                     IVEKDFLANKAGVTVAPYKVMTSSLDLGGLDLTKTYVLKTATGGYDGHGQKIIRSAED
                     LPEAQQLANSAQCVLEEFVNFDLEISVIVSGNGEDVTVFPVQENIHCNNILSKTIVPA
                     RISDQLADKAKKMAVQIAKKLQLSGTLCVEMFATADDIIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPTIHLHAPAVMLNVLGQHVQQAIDYVTENPSAHLHMYGKLE
                     AKHNRKMGHVTLFAEDADEVEEF"
     gene            1685..3014
                     /locus_tag="VGT87_001720"
                     /pseudo
     CDS             1685..3014
                     /locus_tag="VGT87_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184068.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1050 a          608 c          734 g          962 t
ORIGIN      
        1 gcacctagtc gccctgacgt gggttcgtct tcgaaatcaa agatttcgga cttaccgcat
       61 atttttctac ggaaagtttc gacaagtcga aacgcccctt tatatcttaa ttatgaaaac
      121 tccaattatt tctattatca tgggctccaa atctgactgg gcaaccatgc aaaaaacggc
      181 tgaagttcta gacaactttg gcattgctta cgagaagaaa gtcgtctctg ctcaccgcac
      241 gcccgacctc atgttcaagc atgctgagga agcacgtggt cgtggcatca aaatcatcat
      301 cgcaggagca ggtggggcag ctcacttgcc tggtatggtg gcagctaaaa caacgctgcc
      361 tgttatcggt gtgcctgtca aatcacgtgc cctttcaggt ttggattcac tctattccat
      421 tgtgcagatg cctggcggtg tgcctgtggc aaccatggct atcggagaag cgggagcgac
      481 taacgctgcc ctgacagccc tccgtatcct ttctatcgag gaccaaaatc tggctgatgc
      541 gctagctcat ttccatgagg aacaaggaaa aatcgcagag gagtcaagtg gtgaactcat
      601 ttaagaccat tgggattatt ggtggtggtc agctagggca gatgatggcc attgcggcca
      661 tctatatggg ccacaaggtc atcacgctgg atcctactaa cgactgccct gcctcccgcg
      721 ttagcgaggt aattgtggcg ccttacgatg atgttgaggc tttgggacaa ttggctgcgc
      781 gttgcgatgt gttgacttat gagtttgaga atgttgatgc ggacggtttg gatgccgttg
      841 tgtcagcttg tcagctaccg caggggaccg atctgctccg catttcccaa aaccgtatcg
      901 ttgaaaaaga ctttctggca aataaggctg gtgtgacagt cgctccctat aaggtgatga
      961 cgtctagcct tgacctaggg gggcttgatt tgaccaagac ctatgtcctc aagacagcga
     1021 caggtggtta tgacggtcat gggcaaaaga ttatccgctc agcagaagat ttgccagagg
     1081 cgcagcaatt agctaactca gctcagtgtg tcttggaaga gtttgtcaat ttcgaccttg
     1141 aaatctcagt tatcgtgtct ggaaacggag aggatgtgac ggtctttccc gttcaggaaa
     1201 atatccactg caataatatc ctgtcaaaaa ctatcgtgcc agctcgcatc tcagatcaac
     1261 tggctgataa ggctaagaaa atggcggtgc agattgccaa gaaactccag ctgtcaggaa
     1321 ctctctgtgt ggaaatgttt gcgaccgcag atgatatcat tgtcaatgaa attgccccac
     1381 gtccccacaa ctcagggcac tactcaatcg aggcttgcga cttttcacag tttgacaccc
     1441 acattttggg cgtgctagga gcgccgctcc caaccatcca cctccatgct ccagccgtca
     1501 tgcttaatgt cctcggacaa cacgtccagc aggcaattga ctatgtgact gaaaacccta
     1561 gcgcccacct ccacatgtat ggtaaactag aagcgaaaca taaccgcaaa atgggacatg
     1621 tgacactatt tgcagaagat gcggatgagg tggaggagtt ttagaacagg agtagattgg
     1681 taagatggag ttaagggaat ttaaagcgtg gttattaaaa aaccattttg aagaagatga
     1741 gttatacaag gaatctataa agtgttatca agtggaagca tataaggctg cctatatttt
     1801 ttcgtattta gcaaatcata agttcattgc taaattagct attgactaca gtggtgtccc
     1861 atcaaatttt atgtctaaat ttcccgatga tgaagttaga aagtcgagtt ggcaaaaacg
     1921 tgtgaattcc ttaaaaaatg aagatgagtg ggagcagacg gttaacgaaa aattaattca
     1981 agcatgggaa gatgataatg tattcaaatt acctttcaaa attaaagaaa aatattctca
     2041 tatgagggta ttgcgtaata atgcagccca tgcgaaagac cgtcgaattt ctgaatcaac
     2101 agttttagaa ttatggaatg aaattgagta tatatatcct tactttgtaa tcaacggaac
     2161 gattgatgca tggatggaag atttagaaaa actagttaga tattcaaata gtagcataga
     2221 atctcaaaac caattacaga ataaatttgg agatttttct actgaaaata agaccttaat
     2281 tgttaaaaag attatacaaa aatatttggt agattctgaa tgggatgaaa cgtacccaga
     2341 tatagtgatg aattttttat tatatattta attatattaa tcaaattggt aaagacttaa
     2401 cggaaaaaga ggaattgtat ctgtctattg gaacaggtat ttatcatttc caagctgaag
     2461 ctattgatat tttttctcaa tttgaagagt ctctgagtga ttatacattt agatattttt
     2521 gtgaagaatt ttcggataat ttttggaagt ttattgattc tatggattcc gaagttaata
     2581 aagagaactt gacagctact agtttagaaa ttttgaagaa aaattctgct attttagtaa
     2641 atgcagaatt taatgagagc cgatttttat ctgataatag tctttttttc aaatctgtgt
     2701 tagataaaat taagcattta tattactata ctatgcatca tacgggtcaa cgtcatcata
     2761 atacaaatac ttttgactat tcaaaattta attcatatat tccttatatt aactatatat
     2821 tttataggat atcgtcaaat cctggattaa aagaagatga aaaagtaata gattttgtta
     2881 agaggtgtaa taatttaatt tcaagtgact atagtgcaga atcttggtat agtgagcgtc
     2941 aaatgcagga tgtaattaaa aaaattaatg aaaaatacac tatattggaa gatagtggaa
     3001 gggagtcgga ttaatcaaat taaatttggt agagagttta ggaaatctaa cttactcatt
     3061 ctaaatgcta aggaaattaa gttaaaaaat agaaaggaac aagggtagtc gtattcgcta
     3121 aactgaatac aggctacgga cttccttaac tcacagaatt ttaagcaata caagaaagga
     3181 gtgagggtgt ttccgagtat ttgaactcgg gctaacttat ctcctcaata caaatcaaat
     3241 aatagaaagg aaatgggcac tagtttcact aaactgaacc cgagcggaaa gctcggaatt
     3301 tagataaacc gcctaggacg caagcgtcca ttgttggttt cctaaaattc agtc
//
LOCUS       DAUCLR010000122         3095 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.124, whole genome shotgun sequence.
ACCESSION   DAUCLR010000122 DAUCLR010000000
VERSION     DAUCLR010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.124"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..2462)
                     /locus_tag="VGT87_001721"
     CDS             complement(<1..2462)
                     /locus_tag="VGT87_001721"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEQ3814547.1"
                     /translation="MTELSPKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIPP
                     PNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLREQGI
                     SRYDLGRDKFLDKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVFV
                     DLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALQVATT
                     RPETMFGDVAVAVNPEDPRYKDLIGKNVILPIVNKLIPIVGDEHADPEFGTGVVKITP
                     AHDPNDFEVGQRHNLPQVNVMNDDGTMNELAGDFAGMDRFEARQATVAKLEELGALVN
                     IEKRVHSVGHSERSGAAVEPRLSTQWFVKMDELAKQAMDNQETDDRVDFYPPRFNDTF
                     LQWMENVHDWVISRQLWWGHQIPAWYNAEGEIYVGEEAPEGDGWTQDEDVLDTWFSSA
                     LWPFSTMGWPDTDVEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVLI
                     HGLIRDEEGRKMSKSLGNGIDPMDVIEKYGADSLRWFLSNGSAPGQDVRFSYEKMDAS
                     WNFINKIWNISRYILMNNEGLTLEEAESNVAKVAASEAGNVTDQWILHNLNETIAKVT
                     ENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEAEKVITRSVLLYTLDKIL
                     RLLHPIMPFVTEEIYAQYAQGSIVTVDYPVVRPAFENEAAHKGVESLKDLIRAVRNAR
                     AEVNVAPSKPITILVKTADSELEDFFTSNVNYIKRFTNPEKLEISSAIAAPELAMTSI
                     ITGAEIYLPLADLLNVEEELARL"
     gene            complement(2464..3027)
                     /locus_tag="VGT87_001722"
     CDS             complement(2464..3027)
                     /locus_tag="VGT87_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEQ3814548.1"
                     /translation="MNLIIIGAQASGKMTIGQEVARQTGMTLFHNHDSIDFVLRFMPW
                     SQESTALIERIRFAFFETFAKTGQDMIFTIVIDFNDPNDVAMLEKIQAVFQSYDQEVL
                     FVELKTDIEERLKRNRTENRLKHKPLKRNIEWSEQDIQSTMAYAVFNPEEPPKTLTHY
                     QKINNTQLTAAETAQLIIQKMTHIKEN"
BASE COUNT          817 a          715 c          662 g          901 t
ORIGIN      
        1 aggcgagcga gttcttcttc cacgttaagc aaatctgcta gtggcaagta gatttcagca
       61 cctgtgatga tgctggtcat agccagctct ggcgcagcaa tggctgagct gatttctaat
      121 ttttcaggat tggtgaaacg tttgatgtag ttcacgttgc tggtgaagaa gtcttccaac
      181 tcactatctg ctgtcttcac caaaatggtg attggttttg atggtgccac gttaacttca
      241 gcgcgcgcat tacgaactgc acgaatcagg tctttcaaac tttccacacc tttatgcgca
      301 gcttcatttt caaaagctgg gcgaaccaca ggatagtcca ccgtcacaat agaaccttgg
      361 gcatattgag cgtaaatttc ttctgttaca aatggcatga ttgggtgaag gagacgtaaa
      421 attttgtcca aagtataaag aaggacagag cgcgtgataa ctttctcagc ttcgttgtcg
      481 ctatacagta cttccttggt caattccaca taccagttgg caaattcttc ccagataaag
      541 ttgtaaagaa tgtgtcctgc cacaccaaac tcaaacttgt caaagttttc cgtaacttta
      601 gcgattgttt cattgagatt gtggagaatc cactggtcag tcacatttcc agcttctgag
      661 gcagctacct tagccacatt actttcagct tcttccaagg tcaacccttc gttattcatg
      721 aggatatagc gagaaatatt ccaaatttta ttaatgaaat tccaagaggc gtccattttt
      781 tcgtatgaga aacgcacgtc ttgtcctggg gcagagccgt ttgagaggaa ccaacgtaag
      841 ctatcggcac cgtatttctc aatgacatcc atcggatcaa tcccgttccc caatgattta
      901 gacattttgc gtccttcttc gtcacgaata agaccgtgaa tcaggacatt ttggaatggc
      961 tgacgcccgg taaattcaag cgattggaaa atcatacgtg acacccagaa gaaaatgatg
     1021 tcataacctg ttactaaggt tgaggttggg aaataacgtt tgaaatcttc tacatctgtg
     1081 tcaggccacc ccatagttga gaacggccat aaagcagatg agaaccaagt gtcaagaaca
     1141 tcttcatctt gagtccagcc atcaccttct ggtgcttctt cgccaacgta aatctcacct
     1201 tctgcattgt accaagcagg aatttggtgc ccccaccaaa gctgacgcga gattacccag
     1261 tcatggacat tttccatcca ttgaaggaag gtatcattaa agcgtggtgg gtagaaatct
     1321 actcggtcat ctgtttcttg gttgtccata gcttgtttag caagctcatc catttttaca
     1381 aaccattggg tggatagacg aggctcaacg gctgcacctg aacgttctga gtgacctact
     1441 gagtgaacac gtttttcgat gtttactagg gcgccaagtt cttcaagttt agcaacggta
     1501 gcttgacgag cttcaaaacg gtccatgcct gcaaagtcgc cagctaactc attcatggta
     1561 ccgtcatcgt tcatgacatt gacttgtggt aagttatgac gctgaccaac ctcgaagtcg
     1621 ttaggatcat gggcaggcgt gattttcaca actccagtcc caaattcagg atcggcatgc
     1681 tcgtcaccca cgattggaat caatttattc acgattggta aaataacatt tttgccaatc
     1741 aaatctttgt aacgaggatc ttctggattt acggcaacgg caacgtctcc aaacatggtt
     1801 tctggtcgcg ttgttgccac ttgaagggcg cgtgagccat cttccaacat atagttcatg
     1861 tgatagaaag ctccctcaac atccttgtgg ataacctcga tgtcagaaag ggcagttctg
     1921 gcagcaggat cccagttgat gatgaattca ccacggtaaa tccagccttt tttgtagagg
     1981 tcaacaaaaa ccttgcgaac tgcttttgac aagccctcat caagggtgaa gcgctcacgg
     2041 ctgtagtcca cagaaagccc cattttgccc cactgctctt taatcgtagt ggcatattcg
     2101 tctttccact cccagacctt gtctaggaat ttgtcacgac caaggtcgta acgagaaatc
     2161 ccctgctcgc gcagacgctc ctctacttta gcctgtgtcg caatccctgc atgatccatc
     2221 cctggcaacc aaagcgtatc aaagccctgc atgcgttttt gacgaatgat aatatcttga
     2281 agcgttgtgt cccaagcatg tcccaagtgc aacttaccag tcacatttgg cggtggaatc
     2341 acgatagaat acggttttgc tttttggtct cctgatggct tgaaaacatc tgcatcaagc
     2401 catttttgat agcgacctgc ctcaacctcg gcaggattgt atttaggtga tagttctgtc
     2461 atcttagttc tccttgatat gagtcatttt ttggataata agctgcgctg tttcagcagc
     2521 ggtgagctgg gtattattga ttttttggta atgcgtgagc gttttgggtg gttcttctgg
     2581 attaaaaaca gcatatgcca tcgtggattg aatatcttgt tctgaccact cgatgttgcg
     2641 tttaagaggt ttatgtttga gacgattttc tgtgcgattg cgtttaagac gttcctctat
     2701 atctgttttt aattcgacaa agagaacctc ttggtcataa gactgaaaaa cagcctgaat
     2761 cttttcaagc attgctacat catttggatc attgaagtca attacaatag taaaaatcat
     2821 gtcttgacct gtcttagcaa aggtttcaaa aaaagcaaaa cggatacgtt caatcaaggc
     2881 agtgctctct tgagaccaag gcataaaccg taaaacaaag tcaatagaat cgtgattatg
     2941 aaacaaagtc atgcctgtct gcctggcaac ttcttgccca atggtcatct tgcccgacgc
     3001 ttgagcacca atgatgataa ggttcatttt tgtttctgct cctcccactc acttctcagt
     3061 aacccaaaac gcatgtcgcc acagcggcga ccttg
//
LOCUS       DAUCLR010000123         3095 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.125, whole genome shotgun sequence.
ACCESSION   DAUCLR010000123 DAUCLR010000000
VERSION     DAUCLR010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.125"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..662
                     /locus_tag="VGT87_001723"
     CDS             <1..662
                     /locus_tag="VGT87_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEQ3814549.1"
                     /translation="KEQDFDKKLEEFRTSLKDLEVDEKQIDDALAKAGFSKDSLADIK
                     AKLEDTSETATVTANIVGSTGGTFYNRNRLDGDTDKVITFEQGYIDIAHNGEGFEEGK
                     TYTISFEATCELLRKVGITVTQANMKGARLVLTPKNPKLVVESFGLTKDTETINVYPF
                     SYTVLVTSDWYKSKQIDLNASEVQELALEMAYKDVVDGNNATIAGQWSDSPQMILDGG
                     S"
     gene            662..1771
                     /locus_tag="VGT87_001724"
     CDS             662..1771
                     /locus_tag="VGT87_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_115223326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronoglucosaminidase"
                     /protein_id="HEQ3814550.1"
                     /translation="MTENIPLRVQFKRMSADEWARSDVILLEGEIGFETDTGYAKFGN
                     GKSKFSALKYLTGPKGPKGDTGFQGKTGGTGPRGPAGKPGTTDYNQLQNKPNLDAFAR
                     KQETDSKITELKSNKADKNAVYLKAESNAKLDEKLSLTGGIVTGQLQFKPNSGIKPSS
                     SVGGAINIDMSKSEGAAMVMYTNKDTTDGPLMILRSDKDTFDQSAQFVDYSGKTNAVN
                     IVMRQPSAPNFSSALNITSANEGGSAMQIRGVEKALGTLKITHENPNVKANYDENAAA
                     LSIDIVKKTNGEGTAAQGIYINSSTGTTGKMLRIRNKNEDKFYVGPDGGFHSGANSTV
                     AGNLTVKDPTSGKHAATKDYVDEKIAELKKLILKK"
     gene            1786..>3095
                     /locus_tag="VGT87_001725"
     CDS             1786..>3095
                     /locus_tag="VGT87_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814551.1"
                     /translation="MSRDPTYTINEHDLSFADGRFYVTFKADKSSETVRLNSSCLGNT
                     IIKKLQVEDDNTMHDFVKPKVTTQQAFGLAQQVKELDLQLKDPKSDLWGKIKFNNKAM
                     LVEYANKEMSSAIAQSAEQILLQVKSIDDERYSKFEQTLNGIKQTVKSESVESARTQL
                     ASMFDSRISGLDGKYSRLSQTIDSLSSRLDDGVGNYSTLSQKVSGIDLRVSNAANDVS
                     RLSQTAQGLQSQITNANQNYSSLSQTVQGLQTTVRDNQSNATSRINQLSDLISTKVSK
                     GDVETTIAQSYDKIAFAIRDKLPASKMSGSEIISAINLDRSGVKITGKNITLDGNSYI
                     SNAVIKDAHIANMDAGKINTGYLNANRIATEAITGEKIKMDYAFFNKLTANEGYFRTL
                     FAKDIFATSVQSVTLSASKITGGVLAATNGASQWDLNNANMTFNR"
BASE COUNT         1070 a          568 c          673 g          784 t
ORIGIN      
        1 ctaaagagca agattttgat aaaaaacttg aagaatttag aacgtctctt aaagaccttg
       61 aagttgatga aaagcagatt gatgatgctt tggccaaagc cggttttagc aaggatagct
      121 tagctgacat taaagctaaa ctggaagaca cgtctgaaac tgccacagtt acagctaaca
      181 ttgttggatc aactggcggc acgttttaca atcgcaacag actggatggt gatactgaca
      241 aagttatcac ttttgaacaa ggttatattg acattgccca taatggtgaa ggttttgaag
      301 aaggcaagac atacactatc agctttgagg caacctgcga gctactgcgt aaagtaggaa
      361 tcacagtgac acaggctaac atgaaaggtg ctcgcttagt gttaacacct aaaaatccca
      421 aattagttgt cgagagtttt ggcttgacta aggatactga gactatcaat gtctatccgt
      481 ttagctacac agtgcttgta accagcgact ggtataaatc taagcaaata gatttaaacg
      541 cgtcggaggt gcaggaattg gcccttgaga tggcttataa agatgtggtt gacggtaata
      601 atgccacgat agcagggcag tggtcagaca gcccgcaaat gattttagat ggaggtagtt
      661 aatgactgaa aatataccat taagagtcca atttaagcgc atgagcgctg atgagtgggc
      721 tcgtagtgat gtcatcttgc ttgagggtga gataggtttt gagactgata caggctatgc
      781 taaatttggt aatgggaaaa gcaagtttag cgcactcaaa taccttaccg gaccaaaagg
      841 tcctaaagga gacactggtt tccaaggtaa aactggagga actggtcctc ggggccctgc
      901 tggcaagcct ggaacgacag attataatca actccaaaat aaaccaaatc tagatgcgtt
      961 tgcacgaaaa caagaaactg atagtaaaat caccgaatta aaatcaaata aagcagataa
     1021 aaacgctgtt tacttaaaag cagagtcaaa tgcaaagcta gacgaaaaat tgagtttgac
     1081 aggcggcata gtgacaggac aactacagtt taaacctaat agtggtatta aaccctcatc
     1141 ttccgtagga ggagcgatta acattgatat gtctaaatcg gaaggtgctg ctatggtgat
     1201 gtatacaaat aaagatacta ctgatggacc attgatgatt ttacgttctg acaaagatac
     1261 gtttgatcag tcagctcaat ttgtggatta cagcggtaag actaatgctg taaatattgt
     1321 aatgcgccag ccaagcgcac ctaatttttc ctcggcactt aatataacca gtgccaacga
     1381 aggcggtagt gcgatgcaaa ttagaggcgt cgaaaaagcg ctaggaacgc taaaaattac
     1441 tcacgaaaac ccaaacgtta aggcaaatta cgatgaaaac gctgcagcgt tatccattga
     1501 tattgtcaaa aagacaaacg gtgaaggaac agccgctcaa ggaatctaca tcaattcctc
     1561 cacaggaaca actggtaaaa tgctcagaat cagaaataaa aatgaagaca aattttatgt
     1621 aggtccagat ggcggctttc actcaggtgc aaattcaact gtagctggta atctaacagt
     1681 taaagatcca acatctggaa aacatgctgc gactaaagat tacgtagatg aaaaaattgc
     1741 tgagttaaaa aaactcatac taaaaaaata gattaaggag gataaatgag cagagaccca
     1801 acatatacaa taaacgagca cgacttatct tttgcagatg gtcgttttta tgtgaccttt
     1861 aaggcagata agtcaagtga gactgtgaga cttaacagta gttgccttgg caataccata
     1921 atcaaaaagc tacaggtcga ggatgacaat acaatgcacg actttgtaaa gcctaaagtt
     1981 accactcaac aagcttttgg actagctcag caggtcaaag agcttgattt acagctaaaa
     2041 gaccctaagt cagatttgtg gggcaaaatc aagttcaata ataaggcaat gctagtcgag
     2101 tacgccaaca aagagatgtc aagtgccatt gcgcaatcag ctgagcagat attgttacaa
     2161 gtcaaatcta ttgatgatga acgatattcc aaatttgagc aaactctgaa tggtatcaaa
     2221 caaactgtca aaagtgagtc agttgaatcc gcacgtactc agctagcatc aatgtttgat
     2281 agtcgtatta gtggacttga tggcaaatac agtcgtttaa gccaaacaat tgatagtctt
     2341 agcagtcgtc ttgatgatgg tgttggtaac tactcaacgc tatctcaaaa ggtaagtggc
     2401 attgatttac gagttagtaa tgcagctaat gatgtttctc gattgtctca gacagcacaa
     2461 ggattgcagt cacaaatcac aaatgcaaac caaaattaca gcagtttgtc tcagactgta
     2521 cagggactac aaacaactgt acgtgataat caatcaaatg ctacaagtcg gattaatcag
     2581 ttaagtgacc tgatcagcac aaaggtatca aaaggtgatg ttgagacaac tattgctcag
     2641 agttacgaca agatagcctt cgcaatcagg gataaactcc cagcaagcaa aatgtctggc
     2701 agtgagatta tctcggcaat caatcttgat aggtctgggg ttaaaatcac tggaaaaaat
     2761 atcactcttg atggtaacag ctacatcagc aacgctgtca tcaaagatgc tcatattgct
     2821 aacatggatg ccggtaagat taatactggt tatcttaatg ctaataggat tgcaaccgag
     2881 gccattactg gtgagaaaat taagatggac tatgcctttt ttaataaact cactgctaac
     2941 gagggatatt ttaggacgtt gtttgccaaa gacatctttg caacatcagt ccaatctgta
     3001 acactatcag ctagcaaaat tactggaggt gtattagccg ctacaaatgg ggcaagtcag
     3061 tgggacctaa ataatgccaa tatgaccttt aatcg
//
LOCUS       DAUCLR010000124         2820 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.126, whole genome shotgun sequence.
ACCESSION   DAUCLR010000124 DAUCLR010000000
VERSION     DAUCLR010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.126"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            9..1205
                     /locus_tag="VGT87_001726"
     CDS             9..1205
                     /locus_tag="VGT87_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEQ3814552.1"
                     /translation="MEKTKRYIIATAGILLHLMLGSTYAWSVYRNPILQETGWDQAPV
                     AFAFSLAIFCLGLSAAFMGNLVEQYGPRLTGTVSAILYASGNMLTGLAIDRKEIWLLY
                     IGYGVIGGLGLGAGYITPISTIIKWFPDKRGMATGFAIMGFGFASLLTSPIAQWLIET
                     EGLVATFYLLGLIYLIVMLFASQLIIKPTSAEIAILDKKRLQNNSYLIEGMTAKEALK
                     TKSFYCLWVILFINITCGLGLISVVAPMAQDLTSMSPEMSAIVVGAMGIFNGFGRLVW
                     ASLSDYIGRRVTVILLFLVSIIMTISLIFAHSSLIFMISIATLMTCYGAGFSLIPPYL
                     SDLFGAKELATLHGYILTAWAIAALTGPMLLSITVEWTHNYLLTLCVFIVLYILGLMV
                     ALRLKK"
     gene            1271..1978
                     /locus_tag="VGT87_001727"
     CDS             1271..1978
                     /locus_tag="VGT87_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEQ3814553.1"
                     /translation="MVKSYSKTANHNMRRPVVKEELVHYMRTRQKQTTGFLAELEQFA
                     RQENIPIIQPEVVAYFRFLLQSLQPKHILEIGTAIGFSALLMAENAPDATIVTIDRNR
                     EMIDFAKANFAKYDSRQQIRLLEGDAADILSTLEGNFDFVFMDSAKSKYIVFLPEIFR
                     LLRVGGVVILDDVFQGGDITKPIEDIRRGQRTIYRGLQSLFDATLTHPNLTTSLVPLS
                     DGLLMIRKNQADIVLPD"
     gene            2041..>2820
                     /gene="prsA"
                     /locus_tag="VGT87_001728"
     CDS             2041..>2820
                     /gene="prsA"
                     /locus_tag="VGT87_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEQ3814554.1"
                     /translation="MKNSNKLIASVVTLASVMALAACQSTNDNTKVISMKGDTISVSD
                     FYNETKNTEVSQKAMLNLVISRVFEAQYGDKVSKKEVEKAYHKTAEQYGASFSAALAQ
                     SSLTPETFKRQIRSSKLVEYAVKEAAKKELTTQEYKKAYESYTPTMAVEMITLDNEET
                     AKSVLEELKAEGADFTAIAKEKTTTPEKKVTYKFDSGATNVPTDVVKAASSLNEGGIS
                     DVISVLDPTSYQKKFYIVKVTKKAEKKSDWQEYKKRLKAIII"
BASE COUNT          900 a          452 c          564 g          904 t
ORIGIN      
        1 agtttttcat ggaaaaaaca aaacgttata tcattgcaac agcagggata cttctccact
       61 taatgttggg atcgacctat gcttggagtg tttatcgtaa tccaatttta caagagaccg
      121 gttgggacca agctcctgtc gcttttgcct tttctttagc tattttttgt ttaggtttat
      181 ctgctgcttt tatgggaaat ttagttgaac aatatggtcc tcgtctaacc ggaacggtgt
      241 cagctattct ttatgcttct ggaaatatgc tgactggttt agcgattgat cgtaaagaaa
      301 tttggctttt atatattggt tatggtgtta ttggtggctt aggacttgga gcgggatata
      361 tcacaccgat atcaactatt ataaaatggt ttccagataa aagaggcatg gcaacagggt
      421 ttgctattat ggggtttggt tttgcgtctt tattaacaag tccgattgcc cagtggctaa
      481 ttgaaacaga aggtttggta gcaacatttt accttttagg gctgatttat ctaatagtta
      541 tgctttttgc ttctcaattg ataataaaac caacttctgc tgagatagct atactagata
      601 aaaaaagatt acaaaataac agttatttaa tcgaaggaat gaccgctaaa gaagcactta
      661 aaacaaaatc attttattgt ctttgggtaa ttttatttat caatatcaca tgtggcttag
      721 gacttatctc agttgttgcg ccaatggcac aagatctaac tagtatgagt ccagaaatgt
      781 cagcaattgt ggttggtgct atggggattt ttaacggttt cggtcgttta gtgtgggcta
      841 gtttgtcaga ctatattgga cgccgtgtga cagtaatact tttattttta gtaagcataa
      901 ttatgactat tagtttaata tttgcacata gttccctaat tttcatgata agcattgcca
      961 cccttatgac ttgttatggt gcgggttttt ctctcattcc gccttattta agtgatcttt
     1021 ttggggcaaa ggaactggct acattacatg ggtatatatt gacagcttgg gcaatagctg
     1081 cattaactgg acctatgtta ttatctatca ctgttgaatg gactcataat tatcttctta
     1141 ccttatgtgt ttttattgtc ttatacattt taggattaat ggtagcatta aggttaaaga
     1201 aatgatgtcc tataaaaaat ttaaaagtaa accctagcaa agcctagggt ttttttatat
     1261 aattaggttt atggttaaat catacagtaa aacagcaaat cataatatgc gtcgtcctgt
     1321 tgtcaaagaa gagcttgttc attatatgcg aacacggcag aagcaaacca caggtttttt
     1381 agcagagtta gagcaattcg caagacaaga aaacattcct attattcagc ctgaggtcgt
     1441 ggcttatttc aggtttttat tgcaaagctt acagccaaaa cacattttgg agattggcac
     1501 agccattggt ttttctgcgc ttttaatggc tgaaaatgca ccagatgcga cgattgtgac
     1561 tattgatcgt aatcgagaga tgatcgattt tgctaaagca aattttgcta agtacgacag
     1621 tcgccaacag atccgcctgt tagaaggaga tgcagcagat atattatcta cacttgaagg
     1681 aaattttgac tttgtgttta tggattccgc caaatccaaa tatattgtct ttctaccaga
     1741 gattttcaga ctgttaagag tcggtggtgt agtcattttg gatgatgtct ttcaaggagg
     1801 tgacattaca aaaccgatcg aagacattcg acgaggtcag cgtactattt atcgtggctt
     1861 acagtctttg tttgatgcca ccttaactca tccaaaccta acaactagcc tggtccctct
     1921 tagtgatgga ttattaatga ttcgtaaaaa tcaagcagat attgtattac cagattaagt
     1981 aacattgata aatttatgtt ataataaatt agttaaagat aaaaaggagc tcagaagtaa
     2041 atgaaaaact caaataaact cattgctagt gttgtgacat tggcctcagt gatggcttta
     2101 gcagcttgtc aatcaactaa tgacaatact aaggttattt cgatgaaagg tgatacaatt
     2161 agcgttagtg atttttacaa tgaaacaaaa aacacagaag tatcgcaaaa agcgatgcta
     2221 aatctggtaa ttagtcgtgt ttttgaagct caatatggtg ataaggtttc aaaaaaagaa
     2281 gttgaaaagg cgtatcataa aacagctgaa cagtatggcg cttcattctc tgctgctttg
     2341 gcacaatcaa gcttgacacc tgagactttt aagcgtcaga tccgctcttc aaaattagta
     2401 gaatatgcgg ttaaagaagc agctaaaaaa gaattgacaa cacaagaata taagaaagca
     2461 tatgaatctt atactccaac aatggcagtc gaaatgatta ctttagataa tgaagagaca
     2521 gctaaatcag tcttagagga actaaaagcc gaaggcgcag actttacagc tattgctaaa
     2581 gaaaaaacaa caacacctga gaaaaaagtg acctataaat ttgattcagg cgcgacaaat
     2641 gtaccgactg atgtcgtaaa agcggcttca agtttgaatg agggtggcat atcagacgtt
     2701 atctcggttt tagatccaac ttcttatcaa aagaagtttt acattgttaa ggtgactaaa
     2761 aaagcagaaa aaaaatcaga ttggcaagaa tataagaaac gtttgaaagc tatcattata
//
LOCUS       DAUCLR010000125         2801 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.127, whole genome shotgun sequence.
ACCESSION   DAUCLR010000125 DAUCLR010000000
VERSION     DAUCLR010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.127"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            31..1356
                     /locus_tag="VGT87_001729"
     CDS             31..1356
                     /locus_tag="VGT87_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEQ3814555.1"
                     /translation="MESIENSYGRLLLESQLPDSAKQLAQPLKSVVILRGKMICQRCH
                     YQLDEEARLPSGAYYCRFCLVFGRNQSDKLLYAIPPMHFPKGNYLVWSGQLTAYQEII
                     SQQLLINMQNQKTTLVHAVTGAGKTEMIYAAIEAVINTGGWVCIASPRVDVCVEVATR
                     LSQAFSCSICLMHAESLPYQRAPIIVATTHQLLKFHKAFDLLIIDEVDAFPFVNNIQL
                     HYAASQALKEGGAKILLTATSTRTLERKVNKGEVVKLTLARRFHNRPLVIPKFIRSFN
                     LFKMIHRQKLPPKILKYLKKQRKTGYPLLIFLPTIIMAESVTAILKELLPAEQIACVS
                     SQSQNRKEDITAFRQGKKTILITTTILERGVTFPQIDVFVLGSHHRVYSSQSLVQIAG
                     RVGRSIDRPDGTLYFFHEGISKAMLLAKKEIKEMNYKGYSHDLSTMSTN"
     gene            1328..1993
                     /locus_tag="VGT87_001730"
     CDS             1328..1993
                     /locus_tag="VGT87_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEQ3814556.1"
                     /translation="MICLLCQQISQTPISITEIIFLRRISSPICQQCQKSFQKIGKSV
                     CATCCANSDIIACRDCLKWENKGYNVNHRSLYCYNAAMKAYFSQYKFQGDYLLRKVFA
                     VELADVITKYYKGYIPVPVPVSPGCFRERQFNQVSAILEAANVIYLSLFEKLDNTHQS
                     SRTKKERLLVEKSYRLLKVSNIPDKILIVDDIYTTGSTIIALRKQLAKVANSDIKSLS
                     IAR"
     gene            2073..2621
                     /gene="raiA"
                     /locus_tag="VGT87_001731"
     CDS             2073..2621
                     /gene="raiA"
                     /locus_tag="VGT87_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEQ3814557.1"
                     /translation="MIKFSIRGENIEVTEAIRDYVESKLTKIEKYFAKDQEIDARVNL
                     KVYRERSSKVEVTIPLDSVTLRAEDVSQDMYGSIDLVVDKIERQIRKNKTKIAKKHRE
                     KVPTGQVFTTEFEAEEVDEIPEVQVVRTKNVTLKPMDVEEARLQMELLGHDFFIYTDS
                     EDGATNILYRREDGNLGLIEAK"
BASE COUNT         1008 a          454 c          515 g          824 t
ORIGIN      
        1 aaattagcaa aaaaaagcga ataataaagt atggagtcta ttgaaaatag ttatggacgg
       61 ttgttgctag aaagccagtt accagattca gcaaagcagc ttgctcaacc tttaaaaagt
      121 gtagtaatat tgagaggaaa aatgatttgt caacgttgtc actatcagct tgatgaagaa
      181 gcacgattgc caagtggagc ctattattgc cgattttgtc ttgtctttgg acgaaatcaa
      241 tcagataaat tgctctatgc tatcccacct atgcattttc caaaaggtaa ttatctggtt
      301 tggagcggtc aactaactgc ttatcaagag ataatttccc aacaattatt aatcaatatg
      361 caaaatcaaa aaacaacatt agttcatgct gtaactggtg caggaaaaac agaaatgatt
      421 tatgctgcga ttgaggcggt aattaacaca ggtggctggg tttgcattgc cagcccaaga
      481 gtagatgttt gtgtagaggt ggctactcgt ttatcccaag ccttctcctg cagcatctgt
      541 ttaatgcacg cagagtctct tccttatcaa agagctccta ttatagtagc cacaacgcat
      601 cagctgctaa aatttcacaa ggcttttgat ctcttaatta ttgatgaagt agacgcattc
      661 ccttttgtaa ataatattca acttcattat gctgcaagtc aggctttaaa agaaggtggc
      721 gcaaagattt tattaactgc aacttcaact agaactttag aaagaaaggt aaacaaggga
      781 gaagtagtca agctgacatt ggcgagacgc tttcataata ggccattagt tattccaaaa
      841 tttataagaa gttttaatct atttaaaatg atacatcggc aaaaacttcc tcctaaaatt
      901 ttaaaatact taaaaaaaca aagaaaaaca ggctatcccc ttttaatttt tctacccact
      961 attataatgg cagaatctgt tacagcaatc ttaaaagagt tattgcccgc cgaacaaata
     1021 gcttgtgttt ctagtcaatc acaaaacagg aaagaagata tcacagcttt tcgtcaaggg
     1081 aaaaaaacga ttttaattac aacaacaatt ttagaaaggg gtgtgacatt tccccaaatt
     1141 gatgtttttg ttcttgggtc tcatcatcgt gtctatagtt cccaaagtct tgttcaaatt
     1201 gctggacgag ttggaagatc tatagacaga ccagacggaa cgttatattt ctttcatgaa
     1261 ggtatcagta aagcaatgct actagcaaaa aaagagatta aagaaatgaa ttataaaggg
     1321 tattcgcatg atctgtctac tatgtcaaca aattagtcaa acaccaataa gtattacaga
     1381 aatcatcttt ttaaggcgta tctcttcacc gatttgtcaa caatgtcaaa aaagctttca
     1441 aaagatagga aaaagtgttt gtgcgacatg ttgtgcaaac tcagatataa tagcttgtcg
     1501 agattgtcta aaatgggaaa acaaaggata caatgtaaat catagaagct tatattgtta
     1561 taatgctgct atgaaagcat acttcagtca atataagttt caaggagact atttattaag
     1621 aaaagttttt gcagtagaac ttgccgatgt tatcaccaag tactataaag gctatatccc
     1681 agtcccggtt cctgtaagtc ccggttgttt tcgagaaaga caatttaatc aagtgagcgc
     1741 tattcttgag gcagctaatg ttatctacct ttctcttttt gaaaagctag ataatactca
     1801 ccaatcttcc agaacaaaaa aagagagatt attagtagaa aaatcttatc gactactaaa
     1861 agtatcaaac attcctgata aaatccttat agtagatgat atttatacta ctggtagtac
     1921 aattatcgct cttagaaaac aattggctaa agtagcaaat agtgacatta aaagtttgtc
     1981 aattgcacgt taaaacttgc aaattttcat gaaagcagta taatagtatt aaagataaag
     2041 taagcgcttc aagcgcgaga aagaggtatc ctatgattaa gttcagtatt cgtggagaaa
     2101 atatcgaagt gacagaagct attcgcgatt atgtagaatc aaagcttact aaaatcgaaa
     2161 aatattttgc taaagaccaa gaaatagatg cacgtgttaa tttaaaagtt tatcgggaga
     2221 gatcttcaaa agttgaggta acgatcccat tagattcagt aacccttcgt gctgaagatg
     2281 tttcacagga tatgtacggc tctattgatt tagttgttga taagatcgaa cgccaaatta
     2341 gaaaaaataa aaccaaaatt gctaaaaaac atcgcgaaaa ggttccaact ggtcaagtct
     2401 ttacaactga gtttgaagct gaagaagtcg atgagatacc agaagtacaa gttgtacgta
     2461 ctaaaaatgt gacattaaaa ccaatggatg ttgaagaagc gcgtttacag atggaactat
     2521 tgggacatga tttctttatc tacactgatt ctgaagatgg tgcgacaaac attttatacc
     2581 gtcgcgaaga tggaaaccta ggattaatag aagctaaata gttcacaact aaaacctaca
     2641 gggatgatta tacgtatttt aaaagtggga aaaagctatt catgtttaga ggtgacatga
     2701 tgaaaatgag attaagtgat agatacgaca gtcattatac taacaaaagt atctattaca
     2761 gttcctcttt aagtaaatct tacgtaaaat tcgtttgaaa a
//
LOCUS       DAUCLR010000126         2795 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.128, whole genome shotgun sequence.
ACCESSION   DAUCLR010000126 DAUCLR010000000
VERSION     DAUCLR010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.128"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..244
                     /locus_tag="VGT87_001732"
     CDS             <1..244
                     /locus_tag="VGT87_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="foldase PrsA"
                     /protein_id="HEQ3814558.1"
                     /translation="KVIANALDKANVKIKDKAFANILAQYANLGQKTKAASESSTTSE
                     SSKAAEENPSESEQTQTSSAAEPTETEAQTQEPAAQ"
     gene            631..>2795
                     /gene="alaS"
                     /locus_tag="VGT87_001733"
     CDS             631..>2795
                     /gene="alaS"
                     /locus_tag="VGT87_001733"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEQ3814559.1"
                     /translation="MKELSSAQIRQMWLDFWKSKGHCVEPSANLVPVNDPTLLWINSG
                     VATLKKYFDGSVIPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRN
                     EAIGWGFELLTSPEWFDFPKDKLYMTYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEI
                     GAGPSGPDTEIFFDRGEDFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPNKNIDTGAGLERLAAVMQGAKTNFETDLFMPIIREVEKLSGKTYNPDGDNMS
                     FKVIADHIRALSFAIGDGALPGNEGRGYVLRRLLRRAVMHGRRLGINETFLYKLVPTV
                     GQIMESYYPEVLEKRDFIEKIVKREEETFARTIDAGSGHLDSLLAQLKAEGKDTLEGK
                     DIFKLYDTYGFPVELTEELAEDAGYKIDHEGFKSAMKEQQDRARAAVVKGGSMGMQNE
                     TLAGIVEESRFEYDTYSLESSLSVIIADNERTEAVSEGQALLVFAQTPFYAEMGGQVA
                     DTGRIKNDKGDTVAEVVDVQKAPNGQPLHTVNVLASLSVGTNYTLEINKERRLAVEKN
                     HTATHLLHAALHNVIGEHATQAGSLNEEEFLRFDFTHFEAVSNEELRHIEQEVNEQIW
                     NALTITTTETDVETAKEMGAMALFGEKYGKVVRVVQIGNYSVELCGGTHLNNSSEIGL
                     FKIVKEEGIGSGTRRIIAVTGRQAFEAYRNQEDALKEIAA"
BASE COUNT          871 a          532 c          610 g          782 t
ORIGIN      
        1 caaggttatt gcaaatgcat tggataaagc taatgtaaaa attaaagaca aagcttttgc
       61 taatattttg gcgcaatatg caaatcttgg tcaaaaaact aaagctgcaa gtgaaagttc
      121 aacaaccagc gaatcatcaa aagctgcaga agagaaccca tcagaatcag agcaaacaca
      181 gacatcatca gctgcagaac caactgagac tgaggctcag acgcaagagc cagctgcaca
      241 ataagtagga ttttattgac taatcagtta aaaaaactta taataattta gttaagaatt
      301 gtttatcaag gattagatac agaaaagtga cggttgctgc gagtcattga taattagata
      361 aacatgctac ttaactttac aacaattgat atcgtaaatt aagaatgaca agttcattga
      421 atagaggtgg taccgcggtt ttcgccctct gtgagatgga cttgttttgt attgaggact
      481 atttgaaatc aacgaaagga atagagtaga ctaggctgcc aggcttatag ctgatctcct
      541 agcaagaaag acgagctgtg tcaactggtg tgattacatt actagcttca gcataacact
      601 gtttttctta ctctttgtat ctaaattttt atgaaagaat tatcgtctgc acaaatccgc
      661 caaatgtggt tggatttctg gaaatctaaa ggacattgcg ttgagccttc agctaacttg
      721 gttcctgtga atgacccaac gcttctttgg atcaactcag gtgttgcaac cttgaaaaaa
      781 tactttgatg gttcagtgat tccagaaaat ccacgtatta ccaatgcaca aaaatcaatt
      841 cgtactaatg atattgaaaa tgttggtaaa acagcacgtc accatactat gtttgaaatg
      901 cttggtaact tctcaattgg agactatttc cgtaatgaag ctattgggtg gggatttgaa
      961 ctcttgacaa gtcctgaatg gtttgacttt cctaaggaca agctctacat gacttattac
     1021 ccagatgaca aggattcgta taaccgttgg attgcttgtg gcgttgaacc aagtcacttg
     1081 gtgccgatcg aggataactt ctgggaaatc ggtgctggtc cttcaggtcc agatacggag
     1141 attttcttcg atcgtggtga agatttcgat ccagaaaata tcggacttcg cctcttggct
     1201 gaagatatcg aaaacgatcg ttacatcgaa atctggaaca tcgttctctc acaattcaat
     1261 gctgacccag ccgtaccacg ttcagaatac aaagaattac caaacaaaaa cattgataca
     1321 ggtgctggtc ttgaacgtct tgcagctgtt atgcaagggg caaaaacaaa ctttgaaact
     1381 gaccttttca tgccaatcat ccgtgaagta gagaagttgt caggtaaaac ttataatcca
     1441 gatggcgaca acatgagttt caaggttatc gctgaccaca tccgtgcgct ttcatttgct
     1501 atcggtgatg gtgcgcttcc tggaaatgaa ggtcgtggtt acgttcttcg tcgtcttctc
     1561 cgtcgtgcgg ttatgcatgg ccgccgtctt ggcatcaacg aaactttcct ttacaaattg
     1621 gttccgactg ttggacaaat catggaaagc tactacccag aagtgcttga aaaacgtgat
     1681 tttatcgaga aaatcgttaa acgtgaggaa gaaacatttg ctcgtactat cgatgcaggt
     1741 agcggtcact tagattcatt gcttgcgcag cttaaggctg aaggtaagga tactcttgaa
     1801 ggtaaagata tcttcaaact ttatgatact tatggattcc cggttgaatt gacagaggaa
     1861 ttggcagaag atgcaggcta caagattgac cacgaaggct ttaagtcagc catgaaagaa
     1921 caacaagacc gtgcgcgtgc agctgttgtt aagggtggtt caatggggat gcaaaatgaa
     1981 accctagctg gtattgttga agaatcacga ttcgaatacg acacatatag tcttgaatca
     2041 agtctttcag tcatcatcgc tgataatgaa cgtaccgaag ctgtttcaga aggtcaagcc
     2101 cttcttgtct ttgctcaaac accattctat gctgaaatgg gtggacaggt tgctgacaca
     2161 ggtagaatca aaaatgataa gggtgacaca gttgctgagg ttgttgatgt tcaaaaagca
     2221 ccaaatggtc aacctctaca cactgtaaac gttttagcat cactttcagt tggaacaaac
     2281 tacacacttg aaatcaacaa agagcgtcgt ttggctgttg agaaaaacca cacagctact
     2341 cacttgctcc atgcagctct tcacaatgtt atcggtgaac acgcaactca ggctggttca
     2401 ttgaacgaag aagaattctt gcgctttgat tttactcact ttgaagcagt aagcaatgag
     2461 gaacttcgtc acattgaaca agaagttaat gagcaaattt ggaacgctct tacaatcaca
     2521 acgactgaaa ctgacgttga aaccgcaaaa gagatgggag caatggcgct ttttggtgag
     2581 aaatatggta aagtggttcg tgtggttcaa attggtaatt attctgttga actttgtggt
     2641 ggaactcact taaataattc ttcagaaatc ggtctcttca agattgtcaa agaagaaggt
     2701 attggttcag gcactcgtcg tattattgca gttactggta gacaagcttt tgaagcttat
     2761 cgtaaccaag aggatgccct aaaagagatc gctgc
//
LOCUS       DAUCLR010000127         2747 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.129, whole genome shotgun sequence.
ACCESSION   DAUCLR010000127 DAUCLR010000000
VERSION     DAUCLR010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.129"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(14..910)
                     /locus_tag="VGT87_001734"
     CDS             complement(14..910)
                     /locus_tag="VGT87_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEQ3814560.1"
                     /translation="MNEMLILRLIQALLVSAMLFIFFMLVKHLKKNKINPFKRFWTGF
                     WIGLLTDALDTLGIGSFATTTTCFKLTKLVTDDRQLPGTMTVAHVLPVLIQSLCFIFV
                     VKVEVLTLLAMAAAAFIGAYLGTHITKNWHAPTVQRILGSLLIIAAIIMIIRIIYHPG
                     EHLSDTIHGLHGIWLFVGIGFNFIVGVLMTMGLGNYAPELIFFSLMGLSPTVAMPVMM
                     LDAAMIMTASSSQFIKANRVSWDGFAGIVSGGIIGVLLAVFFLTNLDINSLKLLVIAI
                     VFFTGGMLIRSSFTSHKQQTNR"
     gene            1208..1960
                     /locus_tag="VGT87_001735"
     CDS             1208..1960
                     /locus_tag="VGT87_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEQ3814561.1"
                     /translation="MTLMENITFKTPVLRVNDRDLNIAFYQNNLGLRLVSEENAIAIF
                     SSWGEGQECFVIEESPSVRTRAVEGPKKVNTIVIKTNQPKEIEQLLAHGAHYDTLFKG
                     QNGYAFETISPEGDRFLLHAEQDIKHLQGTDLPSLEKDATFKGLTQFKFDIIVLNVIS
                     EERSKAFYRDLFSDQLPITMDFIQEEGPDLAIDPHIAWDLEILEFQVSKDYDMKVLKA
                     TLEEDGHKVYIDKKHKVLVLSDPSQIEVWFTK"
     gene            1945..2747
                     /locus_tag="VGT87_001736"
                     /pseudo
     CDS             1945..2747
                     /locus_tag="VGT87_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285135.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
BASE COUNT          924 a          476 c          523 g          824 t
ORIGIN      
        1 cagaaacgtt atcttatcta tttgtttgtt gcttatgaga ggtaaaggag gaacgaatca
       61 gcattccacc agtgaagaaa acgatagcga tgaccagaag tttcaagcta ttaatatcta
      121 aattcgttaa gaaaaacaca gctagtaaaa cgccaataat accaccagaa acaattccag
      181 caaaaccatc ccaactgaca cggtttgctt tgataaactg gctactagaa gctgtcataa
      241 tcatagctgc atctagcatc ataacaggca tagcaactgt tgggctaaga cccattaatg
      301 aaaagaaaat caattctgga gcataattgc ctaaccccat agtcatcaag actccaacga
      361 taaaattaaa accaattcca acaaaaagcc agatgccatg gaggccatga atggtgtctg
      421 aaaggtgttc tcctggatgg tagattattc taatgatcat gattatagct gctatgatta
      481 aaagactacc aaggattcgt tggacagttg gagcatgcca gttttttgta atatgagtcc
      541 ccaaataagc tccaataaaa gctgctgcag ccatagcaag taaagttaga acctcaactt
      601 ttacgacaaa aataaagcaa agagattgaa tgagcaccgg aagcacatga gctacggtca
      661 tagtgccagg caattgtcta tcgtcagtga ctaatttagt taacttaaag cacgttgtgg
      721 tagtagcaaa agaccctatc ccaagggtgt caagggcatc tgttagcagg ccaatccaaa
      781 accccgtcca aaagcgttta aaaggattga tcttattttt tttaagatgt tttactaaca
      841 taaagaaaat aaaaagcatt gccgaaacta gcaaagcttg tatcagtcta aggataagca
      901 tttcgttcat aaagactagt ttacaaaaaa aaaccttagc cgtcaacgtg atttacttca
      961 ttaaaacatg ccactatgct tagaagagct ttggttcgat gtattttgtt aaaattaagc
     1021 taaaatatta atattgcatg aaaggggacg ccttcctttt tcccttactg agcttagctg
     1081 ttattgtagt gaatgtttaa aaggctagtt tgtcgctctt tatcaaaaat ctagtaggat
     1141 gaatacgtat aatttcttgt caaatagagg aattttgtct ataatagcag aggattagga
     1201 ggaaattatg actttaatgg aaaatattac ctttaaaact ccagtattac gcgttaatga
     1261 tcgcgacctt aatattgcat tttatcaaaa taatttaggg cttcgtttag tatctgaaga
     1321 aaatgcgatt gctatctttt catcttgggg agaagggcag gaatgttttg taattgaaga
     1381 gtcaccttca gttagaacgc gtgcggtaga aggccctaaa aaggttaata cgattgtcat
     1441 caaaacaaat caacctaaag aaatcgaaca attattagct catggagcac attatgatac
     1501 tctttttaaa ggtcaaaatg gctatgcctt tgagactatt tccccagaag gagaccgatt
     1561 cttgttgcat gctgaacaag atattaaaca tctccaaggt actgatttac catcgcttga
     1621 aaaagatgcg acttttaaag gtttaactca atttaagttt gatattattg ttttaaatgt
     1681 tattagtgaa gaacgctcaa aagcttttta tcgggatctg tttagtgatc agttacctat
     1741 tactatggat tttattcaag aagaaggtcc cgatttggca attgatcccc atattgcttg
     1801 ggatttagaa attttagaat ttcaagtctc aaaggactat gatatgaagg tcctaaaagc
     1861 aactttagaa gaagacgggc acaaggtcta cattgataaa aaacacaaag tattggtcct
     1921 ttctgatcca agtcagatcg aagtatggtt tacgaaatga ctttggcagt cattaaatgt
     1981 attgaaaacc atcttcacaa aaaagtatac aaaggggcca gtctggctct ctttcaatct
     2041 ggtcgatggc aagaatacca tatcggaaca attgatggaa gacgaccagt tgatgctaac
     2101 ttagtttatg acttggctag tgtttcaaaa gttgttggtg tggccacaat atgcaatatt
     2161 tattgaataa tggtacgtta gcattagatg atcccttaaa agtatattat cctagtattg
     2221 ctgatacgac tgtcactatt cgacagttat tgactcatac cagtggtctt gatccttata
     2281 ttcctaacag agatgtttta aatgcacaac aattaagaaa agtacttaat catcttactc
     2341 aaaaagaaaa taaaaacttt tattacacag atgttaattt cctcttgtta ggctttatgt
     2401 tagaagagct ctttagtgaa tctttagacc agatatttga taagactatc tttactccct
     2461 ttgggatgta tcatacgtct tttggccctc gtccagaagc tgtaccaaca ttaaagggtg
     2521 tgagtgatgg agaagttcat gatcccaaag caaaaatctt aaaaaaacat tctggatctg
     2581 cggggctatt ttcaactctt gcagatttag aaagttttag caatcattat cttaatgatc
     2641 cattttcaga ttgtctgtgg cgcaactaca gccaacaaac aattgagcgg tcactagggt
     2701 ggaatttaga tggcgattgg attagtcata caggttacac agggccg
//
LOCUS       DAUCLR010000128         2745 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.130, whole genome shotgun sequence.
ACCESSION   DAUCLR010000128 DAUCLR010000000
VERSION     DAUCLR010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.130"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(14..1390)
                     /gene="mnmE"
                     /locus_tag="VGT87_001737"
     CDS             complement(14..1390)
                     /gene="mnmE"
                     /locus_tag="VGT87_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002888396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEQ3814562.1"
                     /translation="MSITKEFDTITAISTPLGEGAIGIVRLSGTDALAIAQSVFKGKN
                     LEQVASHTINYGHIIDPKTGTIIDEVMVSVMLAPKTFTRENVVEINTHGSIAVTNEIL
                     QLLIRQGARMAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMTIAVKQLDGSLS
                     QLINDTRQEILNTLAQVEVNIDYPEYDDVEEMTTALLREKTQEFQSLLENLLRTAKRG
                     KILREGLSTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNIKGVPLKL
                     VDTAGIRETDDLVEQIGVERSKKALQEADLVLLVLNASEKLTDQDRALLNLSQDSNRI
                     ILLNKTDLEQKIELEQLPDDYIPISVLTNQNINLIEDRINQLFFDNAGLVEQDATYLS
                     NARHISLIEKAVQSLEAVNDGLALGMPVDLLQVDLTRTWEILGEITGDAAPDELITQL
                     FSQFCLGK"
     misc_feature    1559..1694
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            1723..2223
                     /locus_tag="VGT87_001738"
     CDS             1723..2223
                     /locus_tag="VGT87_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEQ3814563.1"
                     /translation="MSEAIIAKKAEQVELIAEKMKAAASIVVVDSRGLTVDQDTVLRR
                     SLRESGVEFKVIKNSILTRAAEKAGLDELKDVFVGPSAVAFSNEDVIAPAKVINDFTK
                     TADALEIKGGAIEGAVSSKEEIQALATLPNREGMLSMLLSVLQAPVRNVAYAVKAVAE
                     NKEGAA"
     gene            2288..2653
                     /gene="rplL"
                     /locus_tag="VGT87_001739"
     CDS             2288..2653
                     /gene="rplL"
                     /locus_tag="VGT87_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEQ3814564.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAAAAA
                     GGAEEAAKDSFDVELTSAGDKKVGVIKAVREITGLGLKEAKGLVDGAPANVKEGVAAA
                     EAEEIKAKLEEAGATITLK"
BASE COUNT          835 a          516 c          577 g          817 t
ORIGIN      
        1 ttagagactg ttcttattta ccaagacaaa attggctaaa gagttgggta atgagttcat
       61 caggagcagc atctcctgtg atttctccga gaatttccca agttctggtt aaatcaacct
      121 gtagtaaatc aactggcatg ccaagcgcca agccgtcatt gacagcttct aaactctgga
      181 ctgccttttc aatcaaagaa atgtggcggg catttgacag gtaagtggca tcttgctcta
      241 ctagtccagc attatcaaag aagagctggt tgatacgatc ttcgatcaga ttaatgtttt
      301 ggttggttaa aacggaaatg ggaatatagt catctggtaa ttgctctaat tcaatctttt
      361 gttccaaatc cgttttattc aacaatataa tacggttact gtcctgactg agatttaaca
      421 aagcccggtc ttggtcggtc agtttttcag aagcattcag cacaagaaga acaaggtcag
      481 cttcttgaag agctttttta gaccgttcaa ctccgatttg ttcaaccaga tcatctgtct
      541 cacgaatacc ggccgtatcg acaagtttta gaggaactcc cttgatatta acgtattctt
      601 caatgacatc tcgggtggtt cctgcaatat cagtcacaat agccttgtct tctcgcaaca
      661 aattattaag taagcttgac ttaccgacgt ttggacgacc aataatagct gttgataagc
      721 cctcacgcag aattttaccg cgtttagctg ttcgaaggag attttccaaa aggctttgga
      781 attcttgtgt tttctcacgt agcagagcag tggtcatttc ttccacatcg tcgtattcag
      841 ggtagtcaat attaacttcg acttgagcta aggtgttgag aatttcttgt cgggtgtcgt
      901 tgataagctg ggaaagggag ccatccagtt gcttgactgc aatcgtcata gccttgtctg
      961 ttttggcacg aataatgtcc atgacagcct cagcttgagt caaatcaact cgcccattta
     1021 agaaggcacg tttggtgaat tcgccaggct ctgccattct agcgccttgg cgaatcaata
     1081 gttgcaagat ttcatttgtc acagcaatac taccgtgcgt attgatttca accacatttt
     1141 cacgggtaaa ggttttgggg gctagcatga ctgacaccat aacttcatcg ataatagttc
     1201 ctgtcttagg atcaatgata tggccatagt taatggtgtg tgaggcgact tgctctaagt
     1261 tctttccctt aaaaacagat tgtgcaatgg caagcgcatc tgtccctgat aaacgaacaa
     1321 tgccaatagc tccttcacca agaggggttg aaatggcggt aattgtatca aattctttag
     1381 ttatactcat gccctctatt ttaaatcaag tcctagtaga aagcaaggta ttattttgta
     1441 tagaaaatag gactagcacc attttgagtt tagctaatac taaaaaaata aatgtaaaaa
     1501 aatcattttt taataatttt tattgacaat agtattataa aagaatataa tgatataggt
     1561 tttgataaaa aactgaccta agacagcagg ggagcatgct cataatattc ctgccgaggc
     1621 acaaaacgtg attaaagaaa ataacgtatt tgtactttcg tgtctaggtt taggtgcgat
     1681 ttttttgtac ctagcccaaa aataaaaacg gaggtaaaac taatgagtga agcaattatt
     1741 gctaaaaaag ctgaacaagt cgaacttatt gctgagaaaa tgaaagcagc agcaagtatc
     1801 gttgttgttg attcacgtgg tcttacagtt gatcaagata ctgttcttcg tcgttcactt
     1861 cgtgaaagtg gcgttgagtt caaagttatc aaaaactcaa ttttgactcg tgcagctgaa
     1921 aaagcaggtc ttgatgagtt gaaagatgta ttcgtaggac catctgcagt agcattttct
     1981 aacgaagatg ttatcgcacc agctaaagtt atcaacgact ttactaaaac tgctgacgca
     2041 cttgaaatca aaggtggtgc aatcgaaggc gctgtttctt caaaagaaga aatccaagca
     2101 cttgcaacat tgccaaaccg cgaaggaatg ctttctatgc ttctttctgt acttcaagct
     2161 ccagtccgca acgttgcata cgctgtcaaa gctgttgcag aaaacaaaga aggcgctgct
     2221 taagacgtct cagagtagct taaggctacg cttaacattt taaattaatt atttggagga
     2281 aatcacaatg gcattgaaca ttgaaaacat tattgctgaa attaaagaag cttcaatcct
     2341 tgagcttaac gatcttgtaa aagctatcga agaagaattt ggtgtaactg cagctgctcc
     2401 tgtagctgct gcagctgctg gtggtgctga agaagctgct aaagattcat tcgacgttga
     2461 attgacatct gctggcgaca aaaaagttgg cgttatcaaa gctgttcgtg aaatcacagg
     2521 tcttggtctt aaagaagcta aaggtcttgt tgatggagca cctgctaacg ttaaagaagg
     2581 cgttgctgct gcagaagctg aagaaatcaa agctaaactt gaagaagctg gagcaacaat
     2641 cactcttaaa taagaagcag atagcaattt gagagcgaaa tcccgattta tcagtttttt
     2701 agagcttata gcgatttgaa gaaactgata aaatgatttg gtttc
//
LOCUS       DAUCLR010000129         2735 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.131, whole genome shotgun sequence.
ACCESSION   DAUCLR010000129 DAUCLR010000000
VERSION     DAUCLR010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.131"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            64..1089
                     /locus_tag="VGT87_001740"
     CDS             64..1089
                     /locus_tag="VGT87_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ3814565.1"
                     /translation="MSEKSLGEVLRESRVGKNITLDDIELKTGISSHYLLAMELDQFK
                     IIPEEKFDQFLKEYADIVELDFNTLKRRYRYQVNSKKDSDIKSVTEIVEEKLSKKRLQ
                     EKLLSHQSMGYGQPLEVSKNKETLVIPVKTVGLDSKSPLKTISPVSQFKSDNRLIPKK
                     NVSRMRRNSTDSRSKTSLFPIVILGTIAFAIISVIILGVWQQFEKSQRAKEAEVALLK
                     ASEQSTNGLKNNSNDRKTQVTTEGSGNYLIATVTKSKETVDISVSLTEAQSSWISLTN
                     SEIGEGGITLTQDSPTYTATLPAEITESLLTLGVTKGVSVTIDGQPVDLSPLTSTDLS
                     YITFKIQ"
     gene            1103..1645
                     /gene="pgsA"
                     /locus_tag="VGT87_001741"
     CDS             1103..1645
                     /gene="pgsA"
                     /locus_tag="VGT87_001741"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEQ3814566.1"
                     /translation="MIKKENIPNLLTLVRIAMIPFFLFITSSSNKVGWHIFAAVIFAI
                     ASFTDYLDGYLARKWHVASNFGKFADPLADKMLVMSAFIMLVGLGLVPAWVSAVIICR
                     ELAVTGLRLLLVETGGKVLAAAMPGKIKTATQMLSIILLLCHWIFLGNVLLYIALFFT
                     IYSGYDYFKGASFLFKDTFK"
     gene            1647..2486
                     /locus_tag="VGT87_001742"
     CDS             1647..2486
                     /locus_tag="VGT87_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEQ3814567.1"
                     /translation="MSAIIELKKVTFNYHKDQEKPTLDGVSFHVKQGEWLSIIGHNGS
                     GKSTTIRLIDGLLEPESGSIIVDGDLLTITNVWEIRHKIGMVFQNPDNQFVGATVEDD
                     VAFGLENKGIAHEDIKERVNHALELVGMQNFKEKEPARLSGGQKQRVAIAGAVAMKPK
                     IIILDEATSMLDPKGRLELIKTIKNIRDDYQLTVISITHDLDEVALSDRVLVMKDGQV
                     ESTSTPEQLFARGDELLQLGLDIPFTTSVVQMLQEEGYPVDYGYLTEKELENQLCQLI
                     SKM"
     gene            2462..>2735
                     /locus_tag="VGT87_001743"
     CDS             2462..>2735
                     /locus_tag="VGT87_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEQ3814568.1"
                     /translation="MSINLQNVSYTYQAGTPFEGRALFNINLDILDGSYTAFIGHTGS
                     GKSTIMQLLNGLHVPTTGIVSVDKQDITNHSKNKEIKSIRKHVGLVF"
BASE COUNT          904 a          429 c          538 g          864 t
ORIGIN      
        1 tcttcccaaa atagatgaaa atctgttata atagggttac tatattattt ttgaggtacc
       61 tgtatgagtg aaaaaagtct tggtgaagtg ttaagagaat cgcgtgttgg aaaaaatatc
      121 acactagatg atatagaatt gaagacaggg atttcttccc attacttgct ggcaatggaa
      181 ttggatcagt ttaaaattat tccggaagaa aaatttgatc aatttttaaa agaatatgct
      241 gacattgttg agttagattt caatacttta aaaagacgat accgttatca agttaattcc
      301 aaaaaagaca gtgatataaa atctgtaact gagattgttg aagagaagtt aagtaagaaa
      361 cgattacaag agaagttgtt atcacatcag agcatggggt atggtcaacc gttagaagtt
      421 tctaaaaaca aagaaacctt agttatccct gtaaagactg ttggtttaga tagcaaatcg
      481 ccactaaaaa cgatatcgcc tgttagccaa tttaaatcag ataacaggtt aattcctaaa
      541 aaaaatgtgt cacgtatgag gcgaaatagc actgacagtc gttctaaaac gtcactattt
      601 cccatagtta tattaggaac gattgctttt gcaattatca gtgttatcat tttaggtgtt
      661 tggcaacaat tcgaaaagag tcaaagagct aaagaagctg aagttgcttt gcttaaagcc
      721 tctgagcagt ctactaacgg tttaaaaaat aattcaaatg atcgcaaaac tcaagtcaca
      781 actgaggggt cgggtaatta tttaatagcg actgttacta aatctaaaga gaccgttgat
      841 atcagtgtgt ctttaaccga ggcacaaagt agttggattt ctctaacaaa ttcagaaatt
      901 ggtgaaggtg gtataacatt gactcaagac tcgccaactt atacagcgac tttacctgca
      961 gaaataacag aatcattgtt gacacttggt gtgacaaaag gtgtatctgt aacaattgat
     1021 ggacaacctg ttgatctatc acctttaaca agtactgact taagttatat cacgtttaag
     1081 atccaataat taaggtagac taatgataaa aaaagaaaat attcctaatc tattaactct
     1141 tgttcgaatc gcaatgattc ctttctttct ttttattact tcctcatcta ataaggtggg
     1201 gtggcatatt tttgcagctg tgatttttgc aattgccagt tttaccgatt atctagatgg
     1261 gtatcttgcg cgtaagtggc atgtagccag taattttgga aagtttgccg atcctctagc
     1321 agataagatg cttgtcatga gtgcctttat catgttagtt ggacttggct tagttcctgc
     1381 gtgggtgtca gctgttatta tttgccgaga attggcagta actggtcttc gattactact
     1441 tgttgaaacc ggaggaaagg tccttgcagc tgctatgccg ggaaaaatca aaacagcgac
     1501 acaaatgttg tctattattt tattactttg ccattggata ttcctaggaa acgtcttact
     1561 ctatattgct ctttttttca ctatttattc tggatatgat tattttaaag gagcaagctt
     1621 tctttttaag gatacgttta aataacatgt cagctattat tgaacttaaa aaagttacat
     1681 ttaattacca taaagaccaa gaaaaaccaa cattagatgg tgtatcgttt catgtgaaac
     1741 aaggtgagtg gctgtctatc attggccata acggatctgg taaatcaacg actattcggt
     1801 taattgatgg attattggaa ccagaatcag ggtctattat tgttgatgga gatctactaa
     1861 ccataactaa tgtttgggaa attcgtcata aaattggtat ggtctttcaa aaccctgata
     1921 atcagtttgt tggagcaact gttgaagatg atgttgcttt tgggcttgaa aataaaggta
     1981 ttgcacatga agatataaaa gagagggtta atcatgcttt agagttagtc ggcatgcaga
     2041 actttaaaga aaaagaacca gcccgtttat ctggtggcca aaaacagcgc gtagctattg
     2101 caggtgcagt tgctatgaag cctaaaatta ttattttaga tgaagctact agtatgcttg
     2161 accctaaagg acgattagag ttaatcaaaa ctataaaaaa catccgtgac gactaccagc
     2221 tgactgttat ttccattact catgacttag atgaagttgc tcttagtgat agagttttag
     2281 tgatgaaaga tggtcaagtg gaatcaacat caacaccaga acaattattt gcaagagggg
     2341 atgaactact ccaattaggt cttgatatcc cttttacaac atctgttgta cagatgcttc
     2401 aagaagaagg ttatcctgtt gactatggat atctcacaga aaaggaatta gaaaatcagt
     2461 tatgtcaatt aatctccaaa atgtaagtta tacttatcaa gcagggactc cttttgaagg
     2521 gcgtgccctt tttaacatta atttagatat tttagatggt tcttatacgg cttttatcgg
     2581 acatactgga tccgggaaat cgaccattat gcaattgcta aatggtttac acgttccgac
     2641 aacgggaatt gtcagcgtag ataaacaaga tataacgaat cactctaaaa ataaagagat
     2701 taaatctatt cgaaaacatg taggattagt ttttc
//
LOCUS       DAUCLR010000130         2651 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.132, whole genome shotgun sequence.
ACCESSION   DAUCLR010000130 DAUCLR010000000
VERSION     DAUCLR010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.132"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..>2651
                     /locus_tag="VGT87_001744"
     CDS             <1..>2651
                     /locus_tag="VGT87_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEQ3814569.1"
                     /translation="YTQSEETVLAQLETSREGLTSAQANERLAEYGRNELDEGEKRSL
                     FMKFLDQFKDLMIIILIVAALLSVLTEGMEGLTDAIIILAVVILNAAFGVYQEGQAEA
                     AIEALKSMSSPLARIRRDGHVTEIDSKELVPGDIVLLEAGDVVPADLRLLEANSLKIE
                     EAALTGESVPVEKDLSTAVSEDAGIGDRVNMGYQNSNVTYGRGIGVITNTGMYTEVGH
                     IAGMLANADETDTPLKQNLDNLSKILTYAILVIAAVTFAVGVFLRGQHPLEGLMTSVA
                     LAVAAIPEGLPAIVTVVLSLGTQVLAKRNAIIRKLPAVETLGSTEIIASDKTGTLTMN
                     QMTVEKVYTNGTLQSSSADIAFDNTTLRVMNFANDTKVDPSGKLIGDPTETALVQFGL
                     DHNFDVREAMVAEPRVAELPFDSDRKLMSTIHKQADGKYFIAVKGAPDQLLKRVTQIE
                     ENGQIRPITDADKKTILDTNKSLAKQALRVLMMAYKYSDALPTLETEIVEANLVFSGL
                     VGMIDPERPEAAQAVKVAKEAGIRPIMITGDHQDTAKAIAKRLGIIEEDGVDHVFTGA
                     ELNELSDEEFQKVFKQYSVYARVSPEHKVRIVKAWQNEGKVVAMTGDGVNDAPSLKTA
                     DIGIGMGITGTEVSKGASDMVLADDNFATIIVAVEEGRKVFSNIQKTIQYLLSANMAE
                     VFTIFLATLFGWDVLQPVHLLWINLVTDTLPAIALGVEPAEPGVMKHKPRGRKSSFFD
                     GGVKEAILYQGAFQTILVLGVYGFALMFPEHTSYHDVHADALTMAYVTLGLIQLVHAY
                     NVKSVYQSIFTVGLFKNKLFNYSIPVAFVALMATVVVPGFNQFFHVTHLTITQWLVVI
                     IGSLLMVVLVELVKAVQRSLGQDEKAI"
BASE COUNT          724 a          512 c          611 g          804 t
ORIGIN      
        1 tacacgcaaa gtgaagagac cgtactggct caattagaga ccagtcgcga aggtcttacg
       61 tctgcccagg caaacgagcg cttagcagag tatggtcgta atgagcttga tgaaggcgaa
      121 aaacgaagtt tatttatgaa attccttgat caatttaagg atttgatgat tattattttg
      181 attgtggcag cccttctttc agttcttaca gaggggatgg aaggtttgac agacgccatt
      241 attatcttgg ctgttgttat cctaaatgct gcttttggtg tttaccaaga aggacaagct
      301 gaagctgcta ttgaagcctt gaaatcaatg tcaagtccac tggctcgtat tcgccgtgat
      361 gggcatgtga ctgagataga ctctaaagaa ttggtccctg gagatattgt cttgctagaa
      421 gcgggagatg ttgtccctgc tgatctgcgt cttttggaag ctaactcctt aaaaatcgaa
      481 gaagcagcgc ttactggaga atctgtaccg gttgaaaaag acttgtcaac ggctgtttca
      541 gaagatgccg gtattggtga tcgcgtcaat atgggatatc aaaactcaaa tgtgacatat
      601 ggtcgtggga ttggggttat cactaacact ggtatgtaca ccgaggttgg gcatattgct
      661 ggtatgcttg ctaatgctga tgagacagat acaccattga agcaaaacct tgacaatctt
      721 tctaagattt tgacttatgc gatccttgtg attgcagcgg tgacttttgc tgttggagtc
      781 ttcttgcgtg gtcaacaccc gcttgaaggc ttgatgactt cagtagcgct tgccgttgca
      841 gctattccag agggacttcc tgcgattgtt actgttgtgc tttctcttgg aactcaagta
      901 cttgctaaac gtaacgccat tatccgtaaa ttaccagctg ttgaaacgct aggatcaact
      961 gaaatcattg cttctgataa aacgggtacc ttgacaatga atcaaatgac cgttgaaaaa
     1021 gtttatacca acgggacact ccaaagttca tcagctgata ttgcttttga caatacgaca
     1081 cttcgtgtaa tgaactttgc taatgatact aaagttgatc cttcagggaa gttaattggt
     1141 gacccaacag aaacagcttt ggtacagttt gggttagacc acaattttga cgtacgtgag
     1201 gctatggtgg cagaacctcg tgtggcagaa ttaccatttg attctgaccg taagttgatg
     1261 tcaactattc ataaacaagc tgatggcaaa tatttcatcg ctgttaaagg tgcgccagac
     1321 caattgttga agcgtgtcac acagattgaa gaaaatggac aaattcgtcc tattacggat
     1381 gctgataaaa aaaccattct tgataccaat aagagtcttg ccaaacaagc tcttcgtgtc
     1441 ttgatgatgg cttacaaata ttctgatgct cttccaacac tggaaacaga aattgttgag
     1501 gctaatcttg tcttctctgg tttggtaggg atgattgacc ctgaacgtcc tgaagcagca
     1561 caggctgtta aagtagctaa ggaagctggc attcgtccaa tcatgattac tggtgaccac
     1621 caagatacag caaaagctat tgcgaaacgt cttggtatta tcgaagaaga tggcgttgat
     1681 catgtcttta caggtgctga actcaacgaa ttatcagatg aagagttcca aaaagtcttt
     1741 aaacaatact ctgtgtatgc ccgtgtatct cctgagcata aagttcgtat cgttaaggct
     1801 tggcaaaatg aaggcaaagt tgttgccatg actggcgatg gggttaacga tgcaccatct
     1861 cttaaaacgg cagatattgg tattggtatg ggaattacag gtacagaggt ttctaaggga
     1921 gcttctgaca tggttcttgc cgatgataac tttgcaacca ttatcgttgc ggttgaagaa
     1981 ggacgtaagg tcttctcaaa tattcaaaaa acaattcaat acttactttc tgctaacatg
     2041 gcagaagtct ttaccatctt ccttgcaacc ttgtttggtt gggatgtgct tcaaccagtt
     2101 cacttgcttt ggattaactt ggtaactgat accctaccag ctattgctct tggtgttgaa
     2161 cctgctgagc ctggtgtgat gaaacacaaa cctcgtggac gtaagtcaag cttctttgat
     2221 ggtggggtga aggaagccat tctttaccaa ggtgccttcc aaaccatttt ggtgcttggg
     2281 gtttatggtt ttgccttgat gttcccagaa cacacaagct accatgatgt ccatgcagat
     2341 gccttgacca tggcttatgt gaccctcggt ttgattcagt tggtacatgc ctacaacgtg
     2401 aaatctgttt accaatccat ctttactgtt gggttgttca aaaacaaatt gttcaactat
     2461 tctattccag tggcctttgt agcattgatg gcaacagttg ttgttcctgg atttaatcaa
     2521 ttcttccatg tgacacactt gacgatcaca caatggttag tggttattat cggaagtctc
     2581 ttgatggttg ttcttgttga attggtgaaa gctgttcaac gatcacttgg tcaagatgaa
     2641 aaagcaattt a
//
LOCUS       DAUCLR010000131         2591 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.133, whole genome shotgun sequence.
ACCESSION   DAUCLR010000131 DAUCLR010000000
VERSION     DAUCLR010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.133"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..2075
                     /locus_tag="VGT87_001745"
     CDS             <1..2075
                     /locus_tag="VGT87_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004818439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HEQ3814570.1"
                     /translation="KEVADIKTSDMLNLPVPEANFEVIKTKPTEEQKEILEAISERAD
                     AVRNNQVEPTEDNMLKITNDGKKLALDQRLINPLLPDDPNSKVNVCVKNIFSIWDKTK
                     ENSSTQLVFSDMSTPKRDGEFNIYDDIRNKLVNMGIPKEEIAFIHEADTDKQKDELFS
                     KVRRGEVRVLLGSTQKMGAGTNVQNKLIALHDLDVPWRPSDLEQRSGRIVRQGNENDK
                     VNIFRYVTENTFDSYLWQTIENKQKFISQIMTSKTPVRVAEDVDEASLSYSEIKALAT
                     GNPLIKEKMDLDNEVTKLKMLEANYKSNKYKLEDKVNKIYPQSILKTEMEIQAVKEDI
                     ASVEKLGEGDSKFTSISLGENKILDKKEAGEKLLEEIKKVKINDSKVIGKYRNLDLQV
                     SYNFMTNAHTFKLLGKAEYFGEFSNSTDGNITRLDNAIEKMPSRLERLNQNLENYKES
                     LENAKVELTKPFEKADELRDKTLRLAEINKLLDMGEVEELENQSPLLEDLKRAIVDYS
                     NYEFSESNSYEDFDKLYPDLSHIGLAYTETPDGKHSIQYEVNLEEKTWTQYVDDVAIR
                     TESFVEEDISNSQALKDMTEAIKMSSFDDLVSVDEEDLKQALGLEIDDDGNFYDPLAK
                     DLDNDGIPDRYDNDFKDSDYFESTYDVEDNLHAREDKPSILGQISKFKSEEEKDKNQE
                     KNEKGQER"
     gene            2119..>2591
                     /locus_tag="VGT87_001746"
     CDS             2119..>2591
                     /locus_tag="VGT87_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEQ3814571.1"
                     /translation="MEYKDIRENLEEMMNDNYKDFIKALVSIEKGVNDEKALEEVYVL
                     FMIKDTTGLLNDDFDYMIDDMKEQGKIVENTNEFEEKDDLINLVGNIAGKVENLEREN
                     ANREKFKVSNFSLVSKDDNGNKIYTNCSAYGDKTKDVENLKQGDFVKIFGQVKTS"
BASE COUNT         1097 a          330 c          489 g          675 t
ORIGIN      
        1 ttaaagaagt tgcagatata aagacctcag atatgttgaa tttgccagtt cctgaagcaa
       61 attttgaagt tattaaaaca aaacctactg aggaacagaa agaaatatta gaagctattt
      121 cagaaagagc tgacgcagtt agaaataacc aagtcgagcc aacagaagat aatatgctaa
      181 agattacaaa tgatggtaaa aaacttgccc ttgaccaaag attaataaac ccactacttc
      241 ccgatgatcc taattcaaag gtaaatgtat gcgtaaaaaa tatcttttcg atctgggata
      301 agacaaaaga aaactcatca acccaattag tattttcaga tatgtccaca ccaaaaagag
      361 atggagaatt taatatctat gatgatatta gaaataagct agtgaatatg ggaataccta
      421 aagaagaaat agcctttatt cacgaagcag atacagacaa acaaaaagat gaattgttct
      481 ctaaggttag aagaggagaa gtaagagtat tattaggctc tactcaaaaa atgggagcag
      541 gtacaaatgt acaaaacaaa ctaatagcct tacacgattt agacgtccca tggagaccgt
      601 ctgacctaga gcaaagaagt ggtagaattg ttcgtcaagg taatgaaaat gataaggtaa
      661 atatctttag atatgtaaca gaaaatacct ttgattctta tttgtggcag acgatagaga
      721 ataagcaaaa attcatttct caaattatga cttcaaaaac tccagtaaga gttgcagaag
      781 atgttgatga agcaagtcta tcttattcag aaattaaggc tttagctacg ggtaatcctc
      841 tgattaaaga aaaaatggat ttagataacg aagttacaaa gctgaaaatg ctagaagcaa
      901 actataagtc taataaatac aagcttgaag ataaggtaaa taaaatttat cctcaaagta
      961 ttttaaaaac tgaaatggaa atacaagcag ttaaagaaga tattgcaagt gttgagaaat
     1021 taggagaagg agatagcaaa ttcacttcaa tcagtcttgg agaaaataaa attttagata
     1081 agaaagaagc aggagagaag ctattagaag aaataaaaaa ggtaaagata aatgatagca
     1141 aggtcattgg caaatataga aatttagact tacaagtttc atataacttt atgactaatg
     1201 ctcacacctt taaacttcta ggaaaagcag aatacttcgg agagttttca aactctactg
     1261 atggtaatat aacaaggctt gataatgcca ttgaaaaaat gccttcaaga cttgagaggt
     1321 taaatcaaaa tcttgaaaac tataaagaat ctctagagaa tgccaaagta gaattaacca
     1381 agccatttga aaaagcagat gagttaaggg ataagacact aagattagct gaaatcaata
     1441 aacttttaga tatgggggaa gtagaagaat tagaaaacca atcaccacta ttagaagatt
     1501 taaagagggc gatagttgat tattctaact acgagttttc agaatctaat agctatgaag
     1561 actttgacaa actatatcct gatttaagcc atataggact tgcctataca gaaacaccag
     1621 atggaaagca ttctattcaa tatgaggtaa atttggaaga aaaaacatgg actcagtatg
     1681 tagatgatgt agctattaga acagaatctt ttgtagaaga agatatatct aactcacaag
     1741 ctcttaaaga tatgaccgaa gccataaaga tgtcaagttt tgatgatctg gtatcagtag
     1801 atgaagaaga tttaaaacaa gccctaggac tagaaataga tgatgacgga aacttctatg
     1861 atccacttgc aaaagatctt gataatgatg gaataccaga taggtatgat aatgatttta
     1921 aagatagtga ttactttgaa tcaacttatg atgtagagga taatcttcac gcaagggaag
     1981 ataaaccatc aatattagga caaatatcaa aattcaaatc ggaagaagaa aaagataaaa
     2041 atcaagaaaa aaacgaaaaa ggacaagaaa gatagaggag ggcgaaagtc tctccttatt
     2101 tagtacaagg aggaaattat ggaatacaaa gatattagag aaaacttaga agaaatgatg
     2161 aatgataact acaaggattt tataaaagca cttgtgagca tagaaaaagg tgttaatgat
     2221 gaaaaggcac ttgaagaagt ctatgtttta tttatgatca aagacacaac aggtctatta
     2281 aatgatgact ttgactatat gattgatgat atgaaagaac aaggtaagat tgttgaaaat
     2341 accaatgaat ttgaagaaaa agatgacctc ataaatctcg tgggtaatat agctggcaaa
     2401 gtagaaaatc ttgaacgaga aaatgctaat agagaaaagt ttaaagtaag taatttttct
     2461 cttgtttcaa aagatgataa tggaaataaa atttatacca attgttcagc ctatggagat
     2521 aagacaaaag atgtagagaa cctaaaacaa ggcgattttg ttaaaatatt tggacaagta
     2581 aaaacaagta t
//
LOCUS       DAUCLR010000132         2490 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.134, whole genome shotgun sequence.
ACCESSION   DAUCLR010000132 DAUCLR010000000
VERSION     DAUCLR010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.134"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(62..757)
                     /locus_tag="VGT87_001747"
     CDS             complement(62..757)
                     /locus_tag="VGT87_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEQ3814572.1"
                     /translation="MKGFINYLKIAVLIILAMVFNVLPMILLQKQHDIPMVLNWGIGI
                     FYLVIVGSVLIVLWRLYQAKQDTPIKQQKMRLVDWGYLALFWLIIRVIAIVGTLVNQL
                     WSGQQVSANDAAIHTLARLIKGGFPLYTALFVLVIAFIAPIMEELVFRGFPMIDLFKG
                     KSLKVAGLVTSLVFALPHATNSVEFIMYSCMGIFLFVAYQRRGNLKDAILLHIFNNLI
                     EVILLMSIGLGVI"
     gene            complement(776..1537)
                     /locus_tag="VGT87_001748"
     CDS             complement(776..1537)
                     /locus_tag="VGT87_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEQ3814573.1"
                     /translation="MKKKKNSFLRLLKMLLLSSLAGGIIGGMVGAFLGYHGERLDQLT
                     FLKDDVINLIILLNRLVVVTGLTLSFVFLTQLKKETAVYNTIEENDYSENGYRQLNKK
                     HAYTMLLIAVASILSMCNVLLGLTLTNDSQHAMLAIPLLDILLLLMVIPFQALAMKRY
                     NAIRGTDVPYFPNLKELKHNIMALDEAELQAYHKTSFESVLSLNGVIIPSLYVILFFV
                     YLFTGQVELTAILVLVLIQLYLLVKSATMTRQFYR"
     gene            complement(1534..1749)
                     /locus_tag="VGT87_001749"
     CDS             complement(1534..1749)
                     /locus_tag="VGT87_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ3814574.1"
                     /translation="MEVILKNRLKELRARDGINQTEMAKLAGVSRQTISLIERNEYTP
                     SVIIAMKIAKVFQEPVEEVFRLVEVEE"
     gene            complement(2110..>2490)
                     /gene="alaS"
                     /locus_tag="VGT87_001750"
     CDS             complement(2110..>2490)
                     /gene="alaS"
                     /locus_tag="VGT87_001750"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661542.1"
                     /note="Catalyzes a two-step reaction, first charging an
                     alanyl molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEQ3814575.1"
                     /translation="QKENAELKEKAAAAAAGDVFKDVQEAKGVRFIASQVDVADAGAL
                     RTFADNWKQKDYSDVLVLVAAIGEKVNVLVASKTKDVHAGNMIKELAPIVAGRGGGKP
                     DMAMAGGSDASKIAELLAAVAEIV"
BASE COUNT          810 a          523 c          404 g          753 t
ORIGIN      
        1 ccaaatgtta gatttttttg tccaactttt ggggtgcagt tcagtcattc gaagcttttt
       61 attatatgac tcccaagcct attgacatta acaaaatgac ttcaatcaag ttattaaaaa
      121 tatgtaacaa gatagcgtct tttaagtttc ctcgtctttg ataggcaaca aagagaaaaa
      181 tgcccataca gctgtacatg ataaattcaa cactattggt ggcatgtggt aaagcaaaaa
      241 caagagaggt cactaaacct gccaccttaa gtgattttcc tttgaagaga tcaatcatag
      301 gaaatcctct aaagactagt tcttccataa taggagcgat aaaagctatc acaagtacaa
      361 atagggcagt ataaagcggg aaaccacctt tgataagtct agctaaggta tgtattgcag
      421 catcattagc actcacttgt tgaccagacc atagctggtt aacaagggta cctacaatag
      481 ctatcacacg gatgattaac caaaataatg ctaaataacc ccagtcaacc aatctcattt
      541 tttgctgttt aataggggtg tcttgcttag cttgataaag acgccataat acaataagga
      601 cacttccaac aataaccaag tagaaaatac caattcccca attaagcacc ataggaatat
      661 cgtgttgctt ttgcaataaa atcatcggaa ggacattgaa aaccatagcc agaataatga
      721 gcactgctat ttttaaataa ttaatgaatc ctttcataaa cagtctcctt ttaggctaac
      781 ggtaaaattg tctggtcatt gttgcactct taacgagtaa atagagctga attaacacca
      841 aaactaagat ggctgttaac tccacttgac cagtgaatag atagacaaaa aataaaatca
      901 catacaagga agggatgatg actccattta atgacaacac actttcaaac gatgtcttat
      961 gataggcttg taactctgcc tcatctagag ccatgatatt atgttttaat tctttcaaat
     1021 ttggaaaata aggaacatca gttccccgaa tggcattgta acgtttcatg gctaaagctt
     1081 gaaaaggaat gaccattaat aataaaagga tatccagaag cggaatggcc agcatagcat
     1141 gctgtgaatc attagttaag gtcagaccaa gtagcacatt gcacatggat aaaatagaag
     1201 ctacggcaat caataacatg gtataagcat gctttttatt cagctgacgg taaccatttt
     1261 cagaataatc attctcttca attgtgttat aaacagctgt ctcttttttc agttgcgtta
     1321 aaaaaacaaa agaaagtgtg aggcctgtga caactactag acgatttaat aagataatca
     1381 ggttaatcac atcatctttt aggaacgtga gctgatctaa tctctctcca tggtaaccta
     1441 aaaaggctcc aaccatgcca cctataatcc ctcccgccaa acttgatagc aataacattt
     1501 ttaacaagcg taaaaaacta ttcttcttct ttttcattcc tcgacctcca ctagacgaaa
     1561 cacctcttca actggttcct gaaatacctt tgcaattttc atggcaatta taactgatgg
     1621 ggtgtactcg ttacgctcaa tgagactgat ggtctgcctc gatactcctg ccagtttagc
     1681 catttccgtt tgattaattc catctctggc tcgtaattct ttgagccgat ttttaagaat
     1741 aacctccatt gccatctcct tcattcttct ttaacttata acttactttc taaaataaga
     1801 aaaagtattc aacagcaagg ctagtcctgt aaagcccaaa accagataac tcaagggtac
     1861 tccaaaccag attaccctga taagatgata acttaaaatg aaaagagtca ggatacttgc
     1921 ccataagcaa atctgcctct taagtttgat accatgtgtc attttctcta acacctcctc
     1981 atgtctattt gttactataa ttgtaacata tctttttctt tttgacaacg ataatagtca
     2041 aaaaggttat gatggtagtc atttcaaata taaaaaacca agttctaaat aacttggttt
     2101 tttatggatt tacactattt cagcaactgc tgctagcagc tctgcaattt tacttgcatc
     2161 gctaccacct gccatagcca tgtctggttt acctccacca cgacctgcta caattggtgc
     2221 caattctttg atcatgttac cagcgtggac atctttggtt ttgcttgcaa caaggacatt
     2281 aaccttctca ccaatagctg ctacgagaac aagcacatca gagtagtctt tttgtttcca
     2341 gttatcagca aatgtacgaa gtgcccctgc atctgcaaca tcaacttgac tagcaatgaa
     2401 gcgcacgccc ttagcttctt gaacatcttt aaagacatca ccagctgctg cagctgctgc
     2461 tttttcttta agttctgcat tttctttttg
//
LOCUS       DAUCLR010000133         2343 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.135, whole genome shotgun sequence.
ACCESSION   DAUCLR010000133 DAUCLR010000000
VERSION     DAUCLR010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.135"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(52..570)
                     /locus_tag="VGT87_001751"
     CDS             complement(52..570)
                     /locus_tag="VGT87_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HEQ3814576.1"
                     /translation="MAKVIFQDNFLLMGTNYHEKEANKVMAEIGKKSPYWDKDKDFIS
                     DYIKSNFKDIYKYYRVSTKDVEIVREPLNRHDPNAIKVMVNKTFVGYFPADLAKRLTP
                     YVKKSSHYQMEATLTGRGGQYKTLKNDLKTVVTKKKDITYKLRLTILKVDRVSKSKNA
                     GLLESIASWFLN"
     gene            complement(582..1337)
                     /locus_tag="VGT87_001752"
     CDS             complement(582..1337)
                     /locus_tag="VGT87_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ3814577.1"
                     /translation="MEQLGDRIRKLREGRNMTQTELSEILGMKTYTTVSKWEKNENFP
                     KGKDLKKLAEIFNVTSDYLLGLTDSKLGKITIQNEQPEIVSIYNQLEQPRQEKVLNFA
                     NEQLEEQNKTVSIFDKKSEETEDYITDYVEGLVAAGLGAYQEDNLHMKVKLRSDDVPD
                     EYDTIAKVAGDSMEPLIQDNDLLFIKVSSQVDMNDIGIFQVNGKNFVKKLKRDYDGAW
                     YLQSLNKSYEEIYLSKDDDIRTIGEVVDIYREY"
     gene            1539..1751
                     /locus_tag="VGT87_001753"
     CDS             1539..1751
                     /locus_tag="VGT87_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ3814578.1"
                     /translation="MVTIAELRAKHNKMSQRELAKELGVTQTSISNWEKDQTKISGEY
                     LISLALFFDVSTDDILGINKQATQKF"
     gene            2021..2332
                     /locus_tag="VGT87_001754"
     CDS             2021..2332
                     /locus_tag="VGT87_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEQ3814579.1"
                     /translation="MPKAEITYRAVDVNETASHGDYKHLIQQWEGLTVATAKQWATEM
                     RDHPDFKQFVLNPTHRIVFIDYKGFKLFVQWKSRNRYKTKKETLPEMLENIKFEKRVG
                     V"
BASE COUNT          765 a          410 c          387 g          781 t
ORIGIN      
        1 ctagattcgc ctcacgctct cctcgccaaa tttgagcgtg gggctttttt gttagttgag
       61 aaaccaagat gctatcgact ctaataatcc agcattttta ctttttgata ctctatctac
      121 ttttaaaata gtcaatcgta acttataagt tatatctttc tttttagtaa ccacggtctt
      181 taaatcgttt ttgagtgttt tgtattgtcc accacgacca gtaagggttg cttccatttg
      241 gtaatgacta gattttttta cataaggggt taatcgttta gctaagtctg ctggaaagta
      301 gccaacaaaa gttttgttta ccatgacttt tatggcattt ggatcgtgac gattaagagg
      361 ttctctgaca atctctacat cttttgttga gaccctgtaa tacttgtaga tatccttgaa
      421 gtttgatttt atgtagtcag aaataaaatc tttatcctta tcccagtatg gtgatttctt
      481 accaatttca gccataactt tattagcttc tttttcatga taatttgttc ccattaagag
      541 aaagttatct tggaatatga cttttgccat aacagtctcc tttaatattc cctataaata
      601 tccacaactt caccgattgt gcggatgtcg tcatcctttg atagatagat ttcctcatag
      661 cttttattta aactttgcaa gtaccaagcg ccatcataat cacgcttgag tttttttaca
      721 aagtttttac cattgacttg gaatatccct atatcattca tatcgacttg gctagatacc
      781 ttgatgaata gcaaatcgtt gtcttgaata agtggttcca ttgaatcgcc agctactttt
      841 gcaatagtat catactcatc ggggacatca tcagaacgta gtttaacttt catgtgtaaa
      901 ttgtcttctt ggtatgcccc aagaccagcg gcaaccaatc cctcaacgta gtcagtgata
      961 taatcttctg tctcctcaga ttttttatcg aatatagaaa cggttttatt ttgttcttct
     1021 aattgttcgt tagcgaagtt gaggactttt tcttgtctag gttgttctag ttggttgtat
     1081 atagaaacga tttcaggttg ttcgttctgt attgtgattt tcccaagttt actatctgtg
     1141 agtcctaaaa gatagtcaga ggtaacgttg aaaatctctg ctagtttttt taggtcttta
     1201 ccttttggaa aattttcatt cttctcccac tttgaaacag tagtataggt tttcatgccc
     1261 aatatttctg aaagttcagt ctgagtcatg ttgcgccctt ctctcaactt tcttattcgg
     1321 tcccctaatt gctccatagg atacctcctt tttaattttg ataagtaaat tatatcaaaa
     1381 ccatgattat aaatcaacca tataagaaaa aagttttaaa taaactatat gatttacgat
     1441 tttttcaaaa aaacagaaaa taaatcatat aaaacacttg acgcaagatt taaaatcata
     1501 tagaatgtaa tcatcatcaa gaaaggagcg actcagctat ggtaactatt gcagaattaa
     1561 gagctaaaca taataagatg tcacagcgtg aattggcaaa agaattaggt gttactcaaa
     1621 catctatcag caactgggaa aaagaccaga caaagatttc aggtgagtat cttattagtc
     1681 tagctttgtt tttcgatgta tctaccgatg acattcttgg gataaacaag caagcaacac
     1741 aaaaatttta aacactatat gattttaaat catatagtag aaaggacaca catgaacgaa
     1801 ataacattta ccctatattg cacaacatct gaagaagcaa tcacggaagt aaagaaacta
     1861 aaagaagccc atccaaagga tagacttcga ttcaatgtaa acattaagcc tgagttttac
     1921 taatagaacc aacttaatta gataataaaa agtccgacgg gaatcggact ccaaacaaaa
     1981 aatatttact taattataac acaagagaga ggatattaat atgccaaaag cagaaattac
     2041 ttatagagca gttgatgtta atgaaacagc ttctcatggt gattataaac acttgattca
     2101 acaatgggag gggttgacag ttgctactgc aaagcagtgg gcgacagaaa tgcgtgatca
     2161 tccagacttt aaacagtttg tattaaatcc aacgcataga atcgtattca ttgattacaa
     2221 aggattcaaa ctgtttgtac agtggaaatc tcgcaatcgt tacaaaacaa aaaaagagac
     2281 actgccagaa atgcttgaaa atattaaatt tgaaaaaaga gtaggagtat aacatgaaca
     2341 aat
//
LOCUS       DAUCLR010000134         2275 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.136, whole genome shotgun sequence.
ACCESSION   DAUCLR010000134 DAUCLR010000000
VERSION     DAUCLR010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.136"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            94..246
                     /locus_tag="VGT87_001755"
                     /pseudo
     CDS             94..246
                     /locus_tag="VGT87_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436790.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(293..823)
                     /locus_tag="VGT87_001756"
     CDS             complement(293..823)
                     /locus_tag="VGT87_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HEQ3814580.1"
                     /translation="MSNFISPVYEIKSIPIEKISPNDYNPNSVAPPEMKLLYDSIKSD
                     GYTMPIVCYYDKEEDRYSIVDGFHRYRIMLDYSDIYERESGRLPVSVIDKSLDYRMAS
                     TIRHNRARGSHDVDLMSQIVKDLHECGRSDNWIAKHLGMDKDEILRLKQITGLASLFK
                     DHEFNQSWEPEELLNE"
     gene            complement(816..2150)
                     /locus_tag="VGT87_001757"
     CDS             complement(816..2150)
                     /locus_tag="VGT87_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HEQ3814581.1"
                     /translation="MKKYLNQNVYDALIERLHFLFNEFPIVYISFSGGKDSGLLLNIL
                     LDFRDKYYPDREIGVFHQDFEAQYSLTTKYVQETFTSLEGRKKVSLYWVCLPMATRTA
                     LSSYEMFWYPWDDKTEDIWVRPMPSQDYVINLENNSITTYRYKMNQEDLAKQFGRWYK
                     QIHGNQKTVCILGNRASESLHRYSGFINKKYGYQKECWITKQFKDVWTASPLYDWSVE
                     DIWHAYYKFSYSYNELYDLFYKAGLKPSQMRVASPFQDYAVDSLNLYRIIDQETWVKL
                     LGRVQGVNFSNIYGRTKAMGYKSIALPKGHSWKSYTQFLLSTLPVRLRNNYVRKFNKS
                     IDFWHKTGGGLTEETINELIEKGYRIARNGISNYTSFKHSRVIFLDQIPDDTDDIVTT
                     KDVPSWKRMCFCILKNDHICRTMGFGLTREQQKIVAFLKEKYKTSLKELESE"
BASE COUNT          739 a          462 c          282 g          792 t
ORIGIN      
        1 ggtatgaagt ccccttatac acatactttc aaaacagctc tcaatcctct gtccgctaca
       61 aaaatacgtc accgctgccc tccggctgct tcattcaacg acaaacttga agccacaaac
      121 agactcatca agggcattaa gtacaatgcc tttagatttc agaactttga caactttaaa
      181 accaaaatcc tcatcgcttt gaacatccaa aaagagagaa ccaatctgat tctctctcgt
      241 tgttaatgat aagtcaccca ctacagttga caatgagtct cttacttcct gtttactcat
      301 ttaatagctc ttctggttcc caagattgat taaactcatg gtccttaaaa agagaagcca
      361 atcctgttat ttgtttcagt cttaaaattt catctttatc cattcctaaa tgttttgcaa
      421 tccagttatc acttcgccca cactcatgaa gatcctttac aatctgactc atcaaatcaa
      481 catcatgact tcctctagct ctattatgtc gaatggtaga agccattcga tagtctaatg
      541 acttatcaat aacagaaaca ggtaatctcc cactttccct ttcataaata tctgaataat
      601 ctagcattat acgatatcta tgaaaaccat caacaatact ataccgatcc tcttccttat
      661 cgtaatagca aacaatcggc atagtataac catcagattt tatactatca tacaataatt
      721 tcatttcagg cggagcaact gaattcggat tataatcatt tggagaaatc ttttcaattg
      781 gaattgattt aatctcatag actggactaa taaaattact cactttctaa ctcctttaaa
      841 ctcgtcttat atttctcttt taaaaaggct actatttttt gttgctctct tgtcaaacca
      901 aaccccatag ttcgacaaat atgatcattt tttaaaatac agaaacacat tcgtttccag
      961 cttggtacat cctttgttgt tacaatatcg tctgtatcat caggaatttg atctaaaaaa
     1021 ataacccttg aatgtttaaa agaggtataa tttgaaatac catttctagc aatacgatat
     1081 cctttttcaa ttagttcatt aattgtttcc tccgtaagtc ctccgcctgt tttatgccaa
     1141 aagtctattg atttattaaa ttttctaaca taattatttc ttaacctcac tggtagcgtt
     1201 gataaaagaa actgagtata cgacttccac gaatgaccct ttggtaaagc aattgattta
     1261 tatcccattg ctttagtacg tccataaata ttcgaaaagt taactccttg aacacgtccc
     1321 aaaagcttta cccaagtttc ttgatctatt attctataaa gatttaggct atcaacagca
     1381 tagtcttgaa aaggagatgc aactctcatt tgtgatggtt ttaaacccgc tttatagaat
     1441 agatcataaa gttcgttata agaataagaa aacttatagt aagcatgcca tatgtcttcc
     1501 actgaccaat catataaggg agatgctgtc cagacatctt tgaattgttt ggtaatccag
     1561 cattcctttt gataaccata ctttttattg ataaaaccac tatatctgtg taaggattct
     1621 gaagcacggt ttcctaatat acaaactgtt ttttgatttc cgtgaatttg tttataccac
     1681 ctaccaaatt gtttcgctaa atcctcctga ttcattttat aacgataagt agtaattgaa
     1741 ttattttcca aattaataac ataatcttga gatggcattg gtcttaccca gatatcttct
     1801 gttttgtcat cccacggata ccaaaacatt tcataactac taagtgcagt tctagtagcc
     1861 atcggtaagc acacccaata caaagaaact ttttttctac cttctaaaga agtgaatgtt
     1921 tcttgaacat attttgttgt taaactgtac tgtgcttcaa aatcttgatg aaacacacct
     1981 atttctctat ccggataata tttatctcta aaatccagta aaatattaag aagtaaccca
     2041 ctatcttttc cacctgagaa ggaaatatag acaataggaa attcattaaa caaaaaatgt
     2101 aatctttcta tcaatgcatc ataaacattt tgatttaaat actttttcac taaacttcct
     2161 cattatgcat ttaaaaatat taatacatag attcactaag aatctctttt tcgatctttt
     2221 tgttttttaa tttttcaagt tgaataatta aaccttgctt ttctttttca aggta
//
LOCUS       DAUCLR010000135         2206 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.138, whole genome shotgun sequence.
ACCESSION   DAUCLR010000135 DAUCLR010000000
VERSION     DAUCLR010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.138"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            68..1323
                     /locus_tag="VGT87_001758"
                     /pseudo
     CDS             68..1323
                     /locus_tag="VGT87_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293804.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1903..2172)
                     /locus_tag="VGT87_001759"
     CDS             complement(1903..2172)
                     /locus_tag="VGT87_001759"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814582.1"
                     /translation="MQKNGVTETDNSYQKEWDRGYDEGYKKGNERNSPKKPEHSPVAD
                     GSGYSDGYSTGYGAGWYDGHLVEAIIDGLISWFNYIFFGSGGSSE"
BASE COUNT          747 a          496 c          323 g          640 t
ORIGIN      
        1 gatgtacaca aaaaatccca tatgacttat aatgcaaagc gaccaaacca tcattagaaa
       61 gaattatatg agacaactct atcatactac agaattgatt ggaattaaag ataaacacat
      121 caccttgaac aaggtttttc aacgcgagac acctatcgaa gtcttcgcca cacttgacta
      181 tcccccacct aagtgtaaac actgtaaagg agttcagatt aaatacgact cccagaaaca
      241 ttctaaaatc ccctttatcg aaattggtgg tttccctagt ctcattcgtt taaaaaggag
      301 aagatctcaa tgcaagtcct gtcgaaaagt ggctgtagcg gaaacaagtc tggttcaaaa
      361 aaaactgtca gatctctgag cacctcagac aaaaagtagc acaaaccttg gttaatcgag
      421 gcgctctcac taacatcgct caacagtcgg ctagatcatc ctctaccgtc taccgaaaac
      481 tcaaccagtt tgtaatcaag gaagacttct ctaaactact cgaggtgttg tcttgggatg
      541 gatttttcta tcaaaagggc gaactcgcct ttatcgctca agattttgag tccaaaaaaa
      601 tcatctccat actggacagc cggcgacaaa caaccattcg aaatcacttc tttagatact
      661 ctaaagaggc tagaaacagt gtcaaaatcg ttaccgttga tatgtcagga agctatattt
      721 cgctcattaa aaagctattt cctcaagcta acatcgtcct agatcgtttc catattgttc
      781 agcacatgaa cagagcgctc agtcaaaccc ggattcacat catgaagcaa tatgacaaaa
      841 agtctctgga atacagagtt cttaaatact actgtcaact cattcaaaag gtagccgaaa
      901 actctcgctc aacgccttct actctagaac ttttagggaa acactaactc ctaaacaatg
      961 tctcaataaa ctatttaatc tcgttcctga attaaaagat tactatgacc tttatcagtt
     1021 actccttttt cacttacaag agaagaatac tgagcatttt ttgacttaat taaaaaggtg
     1081 ttacttctgc tcaatcacac ctttaaaacg gctttgaaca ccctgatgcg atacaaaaac
     1141 tatgtcatta atgccattga gcttccttgt tcaaacgcca aactagaagc aaccaataaa
     1201 ctcatcaaac acatcaaacg caatgctttt ggctttcgga actttgataa ctttaaaaaa
     1261 cgcatttact tcgctttgaa cataacaaaa gagaagacga gtctcgtctc ctcacgtgtt
     1321 tagctataag tcacccacta cagttgacaa agagcccaaa aaagaagata tgatcaagca
     1381 ggcagacaaa actcaccgcc atgcttagga cgggcaacac tttcttttct ggcataaatg
     1441 ctcctttctc aacacacggg tcactaaaac ccaaaaaagc cacccctatc agaggtgacc
     1501 tgaccactaa agggactatc ccaattatag cagatcggtg tgggaagtac tagaatcaaa
     1561 gtaattgatt gtaaagttta taatatatac aaatttacag aattcgatta ttttttacat
     1621 atttcattac aaattaatta agattaaaaa actactttat tgtagtcaaa ttcaactata
     1681 cctctttatt tatttcagat cgttatttaa aatatttgat tttattttac aaaaatacta
     1741 attacactca atatagatca aaaaatattc tgaaatagct cctcatacag tattgtatag
     1801 ttacggtagt cacacctata gaattacgat aaaacttcag attcgtcttt agcgttctta
     1861 ttttctagtt acttaccgaa aagcatagct agatagacat gactactcgg atgaaccgcc
     1921 tgatccaaag aaaatataat taaaccacga tattaaaccg tctataatag cttccactaa
     1981 atgaccatcg taccatccag ctccataacc tgtactataa ccatcactat aaccactacc
     2041 atcagcaaca ggtgaatgtt ctggtttttt tggagaatta cgctcattcc cctttttata
     2101 tccttcatcg taacccctat cccattcttt ctgatagcta ttatcggtct cagtgactcc
     2161 atttttctgc attacagtct gttccgcttc tgggcttttt actatc
//
LOCUS       DAUCLR010000136         2189 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.139, whole genome shotgun sequence.
ACCESSION   DAUCLR010000136 DAUCLR010000000
VERSION     DAUCLR010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.139"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..1912
                     /gene="tet(M)"
                     /locus_tag="VGT87_001760"
     CDS             <1..1912
                     /gene="tet(M)"
                     /locus_tag="VGT87_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEQ3814583.1"
                     /translation="INIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTL
                     LERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGV
                     QAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPN
                     MCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFHNCSLFPVYHGSA
                     KNNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLHLRD
                     SVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQ
                     LKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSR
                     IDKVRYMFNKIT"
     gene            2031..>2189
                     /locus_tag="VGT87_001761"
     CDS             2031..>2189
                     /locus_tag="VGT87_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEQ3814584.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQ"
BASE COUNT          774 a          332 c          437 g          646 t
ORIGIN      
        1 tattaatatt ggagttttag ctcatgttga tgcaggaaaa actaccttaa cagaaagctt
       61 attatataac agtggagcga ttacagaatt aggaagcgtg gacaaaggta caacgaggac
      121 ggataatacg cttttagaac gtcagagagg aattacaatt cagacaggaa taacctcttt
      181 tcagtgggaa aatacgaagg tgaacatcat agacacgcca ggacatatgg atttcttagc
      241 agaagtatat cgttcattat cagttttaga tggggcaatt ctactgattt ctgcaaaaga
      301 tggcgtacaa gcacaaactc gtatattatt tcatgcactt aggaaaatgg ggattcccac
      361 aatctttttt atcaataaga ttgaccaaaa tggaattgat ttatcaacgg tttatcagga
      421 tattaaagag aaactttctg ccgaaattgt aatcaaacag aaggtagaac tgtatcctaa
      481 tatgtgtgtg acgaacttta ccgaatctga acaatgggat acggtaatag agggaaacga
      541 tgacctttta gagaaatata tgtccggtaa atcattagaa gcattggaac tcgaacaaga
      601 ggaaagcata agatttcata attgttccct gttccctgtt tatcacggaa gtgcaaaaaa
      661 caatataggg attgataacc ttatagaagt gattacgaat aaattttatt catcaacaca
      721 tcgaggtccg tctgaacttt gcggaaatgt tttcaaaatt gaatatacaa aaaaaagaca
      781 acgtcttgca tatatacgcc tttatagtgg agtactacat ttacgagatt cggttagagt
      841 atcagaaaaa gaaaaaataa aagttacaga aatgtatact tcaataaatg gtgaattatg
      901 taagattgat agagcttatt ctggagaaat tgttattttg caaaatgagt ttttgaagtt
      961 aaatagtgtt cttggagata caaaactatt gccacagaga aaaaagattg aaaatccgca
     1021 ccctctacta caaacaactg ttgaaccgag taaacctgaa cagagagaaa tgttgcttga
     1081 tgcccttttg gaaatctcag atagtgatcc gcttctacga tattacgtgg attctacgac
     1141 acatgaaatt atactttctt tcttagggaa agtacaaatg gaagtgatta gtgcactgtt
     1201 gcaagaaaag tatcatgtgg agatagaact aaaagagcct acagtcattt atatggagag
     1261 accgttaaaa aatgcagaat ataccattca catcgaagtg ccgccaaatc ctttctgggc
     1321 ttccattggt ttatctgtat caccgcttcc gttgggaagt ggaatgcagt atgagagctc
     1381 ggtttctctt ggatacttaa atcaatcatt tcaaaatgca gttatggaag ggatacgcta
     1441 tggttgcgaa caaggattat atggttggaa tgtgacggac tgtaaaatct gttttaagta
     1501 tggcttatac tatagccctg ttagtacccc agcagatttt cgaatgcttg ctcctattgt
     1561 attggaacaa gtcttaaaaa aagctggaac agaattgtta gagccatatc ttagttttaa
     1621 aatttatgcg ccacaggaat atctttcacg agcatacaac gatgctccta aatattgtgc
     1681 gaacatcgta gacactcaat tgaaaaataa tgaggtcatt cttagtggag aaatccctgc
     1741 tcgatgtatt caagaatatc gtagtgattt aactttcttt acaaatggac gtagtgtttg
     1801 tttaacagag ttaaaagggt accatgttac taccggtgaa cctgtttgcc agccccgtcg
     1861 tccaaatagt cgtatagata aagtacgata tatgttcaat aaaataactt agtgtatttt
     1921 atgttgttat ataaatatgg tttcttgtta aataagatga aatatttttt aataaagatt
     1981 tgaattaaag tgtaaaggag gagatagtta ttataaacta caagtggata ttgtgtcctg
     2041 tatgtggaaa taaaacacga ttaaagataa gggaagatac tgaattaaaa aaattccccc
     2101 tctattgtcc gaaatgcaga caagaaaatt taattgaaat aaagcagttc aaagtaactg
     2161 tgattacaga gccagacgca aagacgcag
//
LOCUS       DAUCLR010000137         2177 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.140, whole genome shotgun sequence.
ACCESSION   DAUCLR010000137 DAUCLR010000000
VERSION     DAUCLR010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.140"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            25..1212
                     /locus_tag="VGT87_001762"
     CDS             25..1212
                     /locus_tag="VGT87_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEQ3814585.1"
                     /translation="MKNIVIVEALRTPIGAFGGSFKSVSAVELGTTVLKKILDKTQVK
                     PEMVDEVILGNVLHAGLGQNVARQIAIHSGIPSDKTAFTLDMVCGSGLKAIQLATQSI
                     MLGDADIVIAGGVENMSQAAYVSTQHRFGQRLGNSQLIDTLVHDGLTDAFNNYHMGIT
                     AENVAQKYGISREEQDQFALESQEKAAKALENHRFADEIVPVSVPQRRKDPLIVTTDE
                     YPKVDTSLEKLQQLRPAFLPKEGTVTAGNASGINDGAALLMLMTEEKALELGLTPLVT
                     IESYASAGVAPELMGAGPIPATQKVLKKAGLTISDLDLVESNEAFAAQSLAVLKDLKL
                     NPEIVNVNGGAIALGHPIGASGARILVTLIHEMKKRQVTRGLATLCIGGGQGTAVIVK
                     NNT"
     gene            1236..1886
                     /locus_tag="VGT87_001763"
     CDS             1236..1886
                     /locus_tag="VGT87_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEQ3814586.1"
                     /translation="MCKEITIKEAVAHVKDGDTIMVGGFMTNGTPEKLIDALVEKGVK
                     DLTLICNDAGFPDKGVGKMVANKQFSTIIASHIGLNREAGRQMTEGETVIDLVPQGTL
                     AERIRSGGFGLGGFLTPTGIGTEVAKGKEVITIDGKDYLLEKPLKADIALIFANKADK
                     NGNLQYAGSENNFNHVMAANAKTTIVEAREVVDVGQMDPNFVHTPGIFVNYLVKGA"
     gene            1888..>2177
                     /locus_tag="VGT87_001764"
     CDS             1888..>2177
                     /locus_tag="VGT87_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-CoA--3-ketoacid-CoA transferase"
                     /protein_id="HEQ3814587.1"
                     /translation="MGTSATVLSKEEIQTRIAKRVAQELEHNTLVNLGIGLPTKVANY
                     IPEGVTITLQSENGFVGLTGLTDDHYDPSIVNAGGQPVSIAPGGAFFDSSTS"
BASE COUNT          683 a          421 c          479 g          594 t
ORIGIN      
        1 taaaaatgaa aggatgttta ttttatgaaa aacatagtta tcgttgaagc tttacgaact
       61 cctatcggag cttttggagg ctcctttaaa agtgtctctg ctgttgaact tggaacaact
      121 gtactgaaaa aaatccttga taaaacgcaa gtaaagcctg aaatggttga tgaagtcatc
      181 ctaggaaacg tccttcatgc aggattaggg caaaacgttg ccagacagat tgctattcac
      241 tctggcatcc ctagtgataa aacagccttc acgcttgata tggtctgtgg ttcaggactt
      301 aaagcaatcc aattagctac gcaaagcatc atgcttggtg acgctgatat cgttattgct
      361 ggtggtgtcg aaaacatgtc acaggcagcc tatgtgtcaa ctcagcaccg ttttggacag
      421 cgacttggta atagtcaatt gattgatact cttgttcatg atggattgac agatgctttc
      481 aacaattatc atatgggtat cactgctgaa aacgttgctc agaaatatgg catctcaaga
      541 gaagagcaag atcaatttgc tttagaaagc caagaaaaag ctgctaaggc attagaaaat
      601 catcgttttg ctgacgaaat tgtccctgtc tctgtgcctc aacgacgcaa agacccttta
      661 attgttacaa cagatgaata cccaaaagtt gacacaagcc ttgaaaaatt acagcaatta
      721 cgtcctgcct tcctccctaa agaaggaacc gttactgctg ggaatgcctc aggaataaac
      781 gatggtgcag ccttattgat gttaatgact gaagaaaaag ctcttgaatt agggttaaca
      841 cctttagtca ctattgaaag ctatgctagt gcaggtgttg cacctgaact gatgggagct
      901 ggaccaattc ctgccactca aaaagtcctt aagaaagctg gattaacaat ttctgatttg
      961 gacttagtgg agtccaatga agcatttgca gcacagtcct tggcggtttt aaaagattta
     1021 aagttaaacc cagaaatcgt gaatgttaat ggaggagcca tcgctttagg tcatcctatt
     1081 ggtgccagtg gcgctcgcat tctagtcaca ttaatccacg aaatgaaaaa acgccaagta
     1141 accagaggac tagcaacact ttgcattggt ggaggacaag gaactgctgt tatcgtgaaa
     1201 aacaatactt agtctcgtga aagttggtag aaaaaatgtg taaagaaatc acaataaaag
     1261 aagcggtagc tcacgttaaa gatggtgata ccattatggt tggtggcttc atgacaaatg
     1321 gcacaccaga aaaattaatt gatgcacttg tggaaaaagg tgtcaaagac ctcactttaa
     1381 tctgtaacga tgccggtttt cctgacaaag gcgttggtaa gatggttgcc aataaacagt
     1441 tttcaactat tattgcatca catatcggtc tcaaccgtga agcgggccga caaatgactg
     1501 agggcgagac tgtaattgat cttgtacctc agggaacatt agctgagcgc atccgcagtg
     1561 gcggttttgg gctaggcggt ttcttaacac ctactggaat aggaaccgaa gttgccaaag
     1621 gaaaagaagt cattactatt gacggcaaag actaccttct tgaaaaacct ttgaaagctg
     1681 atatcgcact tatttttgct aacaaggcag ataaaaatgg caatttgcaa tacgcaggtt
     1741 ctgaaaataa ctttaatcat gtgatggctg ctaatgcgaa aaccaccatt gttgaagcaa
     1801 gagaagttgt tgacgtgggg caaatggatc ctaattttgt ccatactcct ggcatttttg
     1861 ttaactactt agtgaaagga gcctaacatg ggaacatcag ctactgttct ttcaaaagaa
     1921 gagattcaaa cacgtattgc aaagcgtgta gcacaagaat tagaacacaa tacactggtc
     1981 aacttaggca taggtttgcc aacaaaagta gccaactata ttcctgaagg tgtaaccatt
     2041 accctgcagt ccgaaaatgg ttttgtcggt ctcactggat taacagatga tcactacgat
     2101 cctagtattg ttaatgctgg tgggcaacct gttagtattg ctcctggagg ggctttcttc
     2161 gatagctcaa cttcctt
//
LOCUS       DAUCLR010000138         2161 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.141, whole genome shotgun sequence.
ACCESSION   DAUCLR010000138 DAUCLR010000000
VERSION     DAUCLR010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.141"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            458..1966
                     /locus_tag="VGT87_001765"
     CDS             458..1966
                     /locus_tag="VGT87_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEQ3814588.1"
                     /translation="MLDYYLESDIIDQKILLAILLTEKELTMAAACSQTTLTALKIKQ
                     YLRQFNALFKGYLRIELIKSRIYCEVLNDKREAFFYDIFALSDTLKMLTFLLLDNPKH
                     KSIAVYTRKQGISQSKAYRLIHKLKHYLQDIGLNIVDNTVIGDELKIRYLIALLHKEY
                     GIILYDIQPADIETIHAFIFAAQKNLQPSAFLDRRFLFFDVLLMLTWKRHRYPVHLPH
                     LALFEHLKSLPIFDNIKTIAVDELAPRTRVTFSSDDFDYLFLIYLTTDNSFLSGYWTS
                     HQRQQLYHLITKDPDYHLLIHRLQALVGKYSDIHEHIPNLIPFFKRTLYNLQTLITFD
                     GYYFDQYQGNMLLLDKLETVIKDWLYDTGRQGSISSGHLHLMCLYLEQILESSIAPIN
                     ITVIESQETVGNVIANFITSTIPSYKVELSRVNILSDNIYPYDKPVDLVVTSQKLLPF
                     LKELGVFPKETRLFGLSLDCIQQQREDLIKTILALHQNHYQKRLEELWRVPS"
BASE COUNT          654 a          417 c          342 g          748 t
ORIGIN      
        1 aactagctct ctcaaaaaag attaaaaata aaggcattct aaagaatcat ttaagaaaac
       61 tttaaggtta ctatcgtaga atgaaaacat cctaaaaagc ggtttaccta aaccgttggg
      121 agaataggtt ttccccttgt tttccctttt cataaatctc ctgggcacct atcttcttag
      181 gtgccctccc ttgtttaaac ctgaccagtt aaccggctgg ttaggttttt aaatttaaaa
      241 agtcaacagc aaaaatatga tatcacccgc gtgttcttag ctctctcaat tatgggctaa
      301 aaacacgcat caatcaatct attgatgaag tttgctgctt tttgatgatc tttattgtga
      361 ctatactggt aatcctattg attttagttg acttttacgg tagattcgct acaatatata
      421 ggattataca atttaattta aggagagaaa aattacattg ttagattatt atttggaatc
      481 cgatattatt gaccagaaaa tcctactggc tattttgttg acagaaaaag aactgacgat
      541 ggcagcagct tgttctcaaa caactttgac tgctttaaaa ataaagcagt atcttcgaca
      601 atttaacgcc ttatttaaag ggtacttacg tattgagctt attaaatctc gtatttattg
      661 tgaggtatta aatgataagc gagaagcatt tttttatgat atttttgcct tatcagatac
      721 tctaaaaatg ttgacattct tattactaga taatcccaaa cacaaatcaa tcgctgttta
      781 tacacgaaaa caagggatat cccagtcaaa agcttatcgt cttattcata aattaaaaca
      841 ttatttacaa gacattggtt taaatattgt agacaatact gttatcggag atgaacttaa
      901 aatacggtat ttgattgctc ttttacataa agagtacggc attatattat atgatataca
      961 acctgcagac attgaaacga ttcatgcttt tatctttgca gcacaaaaaa acttacaacc
     1021 ttctgccttt ttagatagac gcttcttgtt ttttgatgtg ctgttgatgt tgacttggaa
     1081 gcgccatcgc tatcctgttc atttgcctca tttagctctt tttgaacatc tcaaatcctt
     1141 gcctattttt gacaatatca aaaccattgc cgtggatgag ctagcacctc gaactcgtgt
     1201 cacattttcg tctgatgatt ttgactatct ctttttaatt tatttaacga ctgataactc
     1261 cttcttgagt gggtactgga caagtcatca gcgacaacaa ctttaccatt taatcaccaa
     1321 agatcccgat tatcatttac taatccatcg tcttcaggca ctagttggaa aatattccga
     1381 tattcatgag catatcccta acttaattcc ttttttcaaa agaactctct acaatttaca
     1441 aactttaatt acctttgatg gttattattt tgatcaatat caaggtaaca tgctactact
     1501 agataagctt gaaaccgtta tcaaagactg gctttatgac acaggccgtc aaggcagtat
     1561 tagttctggt cacttacact tgatgtgttt ataccttgag cagatcttgg aaagctctat
     1621 tgctccaatc aatattactg ttattgagag ccaagaaact gttggtaacg ttatagctaa
     1681 tttcatcacc tcaactatcc cttcttacaa agttgaactg tcacgggtca atatcttatc
     1741 tgataatatt tatccctatg ataaacctgt agatctggtt gtaaccagtc agaagctctt
     1801 gcctttctta aaagaactcg gtgtttttcc caaagagacc cgcttgtttg gtttgagttt
     1861 ggactgtatc caacagcagc gcgaagattt aatcaaaacc atcttagcct tgcaccagaa
     1921 ccactaccaa aaacgtttag aagaactttg gagagttcct tcttaatttt ttgacagtcc
     1981 ctcctagata ctttcacagt ctaggggggt ttttaaggtt gtcacttgca accttgataa
     2041 gctctctttt taaccattgc tattcgccta gaaacagcac tggaactgtt ttagcggttc
     2101 aactcctctt gtaggatctt cttccctttt ttgaaggtct tatctgaaat ttggcataat
     2161 a
//
LOCUS       DAUCLR010000139         2140 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.142, whole genome shotgun sequence.
ACCESSION   DAUCLR010000139 DAUCLR010000000
VERSION     DAUCLR010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.142"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            72..1460
                     /gene="rlmD"
                     /locus_tag="VGT87_001766"
     CDS             72..1460
                     /gene="rlmD"
                     /locus_tag="VGT87_001766"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEQ3814589.1"
                     /translation="MVSPRKGKRIRMLKKNDIIQVAISDLSHEGAGVAKHDGFVFFVD
                     NALPEEVIDMRVLKVNKNSGFGKVEAYHYLSPARNADVNLTYLRTGIADLGHLTYEDQ
                     LTFKKKQVQDSLYKIAGISDVTVESTIGMTEPLAYRNKAQVPVRRVNGQLETGFFRKH
                     SHDLIPISDYYIQDKEIDRLINFTRDLLRRFDIKPYDETEQTGLLRNIVVRRGHYSGE
                     MMLVLVTTRPKVFRVDQVIEKIVEAFPAVVSIIQNINDKNTNAIFGKDFKTLYGKDTI
                     TDSMLGNNYAISAQSFYQVNTVMAEKLYQTAIAFSDLSKDDIVIDAYSGIGTIGLSFA
                     KTVKAVYGVEVIEAAVRDAQHNAALNGITNAYFVADTAEHAMATWAKDGIKPSVILVD
                     PPRKGLTESFIQASVAMGPQKITYVSCNPATMARDIKRYQELGYKLAKVQPVDLFPQT
                     HHVETIALIQKM"
     gene            1691..>2140
                     /locus_tag="VGT87_001767"
     CDS             1691..>2140
                     /locus_tag="VGT87_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019118581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEQ3814590.1"
                     /translation="MNFDYFYNRQSEMYNFIRLPMVLMEDEIFESISIEAKVLYSYML
                     NRMGLSYKNGWIDEDGKVFIYYTIESIKDQFNCASEKANKLIAELDIKSGIGLIEKKR
                     QGLGKPNRIYVKDFMSIFNNIELKNQEVRKTKFQKFDNRNSRDSNIES"
BASE COUNT          741 a          362 c          427 g          610 t
ORIGIN      
        1 tagccaaatt aggtttgcgc taagatatgg tataatggag agtaactaaa gttgctcttc
       61 ttttttgagc aatggttagc cctagaaaag gaaaaagaat aagaatgttg aaaaaaaatg
      121 atattatcca agttgctatt tcagatttaa gccatgaggg agcaggtgtc gctaaacacg
      181 atggttttgt tttctttgtt gataatgcct tgccagaaga agtgattgac atgcgtgtct
      241 taaaggtcaa taaaaacagc ggttttggca aagtagaagc ctatcattac ctgtcgccag
      301 ctagaaatgc agatgtcaat ttaacttatt tgcgaacagg cattgcagac cttggacatc
      361 taacttatga agaccaactg actttcaaaa agaaacaagt tcaggacagc ctctataaaa
      421 ttgctggaat cagtgacgta acagtcgaaa gcacgattgg gatgacagaa cctttagctt
      481 accgaaataa ggctcaagtt cctgttagac gtgttaatgg tcagctagaa acaggctttt
      541 tccgcaagca ttctcatgac ttgattccta tttcggatta ttatattcag gacaaagaga
      601 ttgatcgtct cattaatttt acccgtgatt tattacgccg ctttgatatt aaaccgtatg
      661 acgaaacaga acaaacaggt ctcttgagaa atattgttgt tcgccgaggc cattactctg
      721 gagaaatgat gctggtctta gtgactacac gcccaaaagt tttccgtgtt gaccaagtca
      781 ttgaaaaaat cgttgaagct tttccagcag tggtgtctat tattcaaaat attaatgata
      841 agaataccaa tgcaatcttt ggtaaagatt tcaaaacgct ttacggcaag gataccatta
      901 ctgatagcat gcttggaaac aactatgcca tttctgccca gtctttctac caagtgaata
      961 cggttatggc agaaaaactc tatcaaacag ctatagcctt ttcagacctt tctaaagatg
     1021 acattgtgat tgacgcctac tcaggtatcg gaaccattgg cctatcattt gctaaaacag
     1081 ttaaagctgt ttatggtgtt gaagtgattg aagcagctgt tagagatgct caacacaatg
     1141 ccgctcttaa tggcatcacc aatgcctact ttgtcgcaga taccgcagaa catgccatgg
     1201 caacatgggc gaaagatggc attaaaccaa gtgttattct ggtagacccg ccacgaaaag
     1261 gcttaacaga aagtttcatc caagccagcg tggcaatggg accacaaaaa attacctacg
     1321 tttcctgcaa cccagccacc atggcgcgtg acatcaagcg ttatcaagag ttgggttaca
     1381 aactggccaa agtacagccc gtagatttgt ttccccagac gcatcatgtg gagactatag
     1441 cgttgataca aaagatgtaa atatagaaat cctgcatttt aagcagtttt ataagcattt
     1501 tgtcttcgat aaagatgaag aaggatacgt caaaaagacc caaaaaaact acatggacgt
     1561 aagagtagtt ttgcaactgg tatgaggaga cacaaagaaa aatataataa gataaaaccc
     1621 attttatact ttctacaaga gtagcttaaa taaggctgct ctttttttat ttcaaagaaa
     1681 ggaggcagga atgaattttg attattttta taataggcaa tctgaaatgt ataacttcat
     1741 taggttacct atggtattaa tggaagatga gatttttgag agcatttcta ttgaagctaa
     1801 agttttgtac tcatatatgc ttaatcgaat gggtctttca tataaaaatg gctggataga
     1861 tgaagacgga aaagtcttta tttattacac aattgaaagc ataaaagatc aatttaattg
     1921 tgcaagtgaa aaagcgaata aattaatagc tgagcttgat attaaatcag gaataggact
     1981 aattgaaaag aaaagacaag gactgggaaa gcctaacaga atttatgtta aagattttat
     2041 gagcatattt aataatatag aattaaaaaa tcaagaagtt cgaaaaacaa aattccagaa
     2101 gttcgataat cgaaattcaa gagattcgaa tatcgaaagt
//
LOCUS       DAUCLR010000140         2135 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.143, whole genome shotgun sequence.
ACCESSION   DAUCLR010000140 DAUCLR010000000
VERSION     DAUCLR010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.143"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            135..1490
                     /gene="rlmD"
                     /locus_tag="VGT87_001768"
     CDS             135..1490
                     /gene="rlmD"
                     /locus_tag="VGT87_001768"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEQ3814591.1"
                     /translation="MVVKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIFC
                     QITAVKRNFAEAKLLTVNKASKNRVKPACSVYETCGGCQIMHLAYPKQLDFKDDVIRQ
                     ALKKFKPTGYEQFEIRPTLGMKKPDHYRAKLQFQLRSFGGTVKAGLFSQGSHRLVPID
                     NCLVQDQLTQDIINKITQLVDKYKLPIYNERKIAGIRTIMVRKAQASDQVQIIVVSSK
                     EVRLANFIGELTKAFPQVKTVALNSNRSKSSEIYGDETEILWGQEAIHEEVLDYGFAL
                     SPRAFYQLNPQQTEVLYGEVVKALDVGSKDHIIDAYCGVGSIGFAFAGKVKSVRGMDI
                     IPEAIEDAQKNAKAMGFDNAYYEAGKAEDIIPKWYKQGYRADAIIVDPPRTGLDDKLL
                     KTILHYQPKQMVYVSCNTSTLARDLVQLTKVYDVHYIQSVDMFPHTARTEAVVKLQKR
                     V"
BASE COUNT          693 a          303 c          495 g          644 t
ORIGIN      
        1 tcaattttag tgattttcat actttgatta tatcaaaaat ggtaaaatag aagatgaaaa
       61 tactgctaaa aatgtgttgc ttgtgaatgc ttttggtggt gagattagga tattagagga
      121 actaagggga agagatggtc gtaaaggtta aacagaaaat tcctttaaaa attaagcgaa
      181 tgggaattaa cggtgagggg attggttttt atcagaaaac tttggtattt gtccctggtg
      241 ctttaaaggg agaagatatt ttttgccaaa tcacggcggt taaacggaat tttgcagagg
      301 ccaagctgtt aacggtaaac aaggcttcta agaatcgtgt caaaccagct tgttctgttt
      361 atgaaacttg tggtggttgc caaatcatgc atctagccta tcctaaacag ctagatttca
      421 aagatgatgt gattaggcag gcccttaaaa agttcaaacc aactggctat gaacaatttg
      481 agattagacc gactttagga atgaaaaagc cagatcacta tcgggccaaa ttgcagtttc
      541 agttgcgatc atttggcggg acggtcaagg ctggtttatt ttcccaggga agtcatcgct
      601 tggttcctat tgacaattgc ttggtacagg atcaactaac gcaagatatt atcaataaga
      661 ttacgcagtt agtggacaag tacaagttgc ccatttacaa tgagcgcaag attgctggga
      721 ttcggacgat aatggttcgt aaggcacagg ctagtgatca ggtacagatt attgttgtga
      781 gtagcaaaga agttcggtta gcgaatttca ttggcgaatt gactaaggca tttcctcagg
      841 ttaaaacggt tgccttgaat agtaatcgtt ctaaatcgag tgagatttat ggggacgaaa
      901 cggaaattct atggggacaa gaggcgattc atgaagaagt tttagattat ggatttgcgt
      961 tatcaccgag agctttttac caacttaatc cacagcagac agaagttttg tatggtgagg
     1021 tggtgaaggc cttggatgta gggagcaaag atcatattat tgatgcctat tgtggggttg
     1081 gctcgattgg ctttgctttt gcagggaagg tcaagtctgt gcgggggatg gatattatcc
     1141 cagaagcgat tgaggatgca caaaaaaatg ccaaagccat gggttttgat aacgcttact
     1201 atgaggcagg gaaggcagag gatattattc ctaaatggta taaacaaggt taccgcgcgg
     1261 atgccatcat tgtggaccca ccaagaactg gtttagatga taaattatta aaaacgattc
     1321 ttcattacca gcctaaacag atggtttatg tgtcttgtaa tacctccact ttggcccgcg
     1381 atttggtgca gctaacaaag gtttatgatg ttcactatat ccaaagtgta gacatgttcc
     1441 cccatacagc taggaccgag gctgtcgtga agttacaaaa acgtgtatga ttgagtggca
     1501 ataacaactc aagttttata agctcaggta atgggtttga gcaatagctt gacatagtag
     1561 ggatgtcaaa aacggtttac taaggatagt catattattg ataactctat gtttatttta
     1621 gaaacattca tggttaaaaa agtttgttag tttgtcacta agtagggttt atttgaagag
     1681 caagtacaca cagacaatat tagggtaatt ggttgttgaa aaaaagctgt tttttgctat
     1741 aaattagaaa actgagtggt caggaggtta cattaccaga agttatttta gtatgctaat
     1801 atgaacgatg atatagagga tttatcctga tttaatcttt taagctgtaa gaaacctttg
     1861 caaaaactgt aggctgtgtg agtctttatg tgttagatat taaagataaa tgcaacactt
     1921 ttttataatg agatcaacaa ctgataaatc taagttgata accagtaaaa tgatcggaat
     1981 gtagaaaagt tacttaatca agcttttcgt aatttaactg ttagaatgac agaacttatg
     2041 ataaaataaa gaatatgatt aaaatgaaaa agctcagttt atctattacg gagttcgaaa
     2101 tgaaaagaaa atcgagatgt gataacatat tttga
//
LOCUS       DAUCLR010000141         2092 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.144, whole genome shotgun sequence.
ACCESSION   DAUCLR010000141 DAUCLR010000000
VERSION     DAUCLR010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.144"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            146..2077
                     /locus_tag="VGT87_001769"
     CDS             146..2077
                     /locus_tag="VGT87_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HEQ3814592.1"
                     /translation="MTKKFIKSAMILGLASITISMTQPVEAIANRLEYQAQYERVKEE
                     LDALKATSPKLGERSLYFRLVNRFLGWFGVVWDPLAIKETDHLLDLIGQVNYLVSNTE
                     FQAPEYKHAKNKLDLAVAGYELYLAREKERGDTPDYQVTQLTFKQLETIKTKIREALE
                     TFAQEVKTIQQKNPDLFPHAYVLNAKTSAIDGKAQPFLVNGYYDVFYKQFLFTNKNDG
                     YNYNKEADNLTLYQDLIKELLGLKAEIDRLTKDKTDDSRLETLMWESKFTLSDFRRTS
                     RLSAYYTQEDYVKKVVLSEEAPNLRTAKEKVKTLKGILHDYYVDIKEPEKAKQYHVED
                     ETSPSQPEAPAPGQKPAEDEGSSQATEPANPSKEDSSTDASQGSHDSENPATDNPSQP
                     QVPDQGNHQSQVPNDKPQTDKTDTPNVPASPQDTPNAPAPPQDTPKVLEAGGQSGPAG
                     NAEEKAPDSGPKESGQSSSKESPSVGEDTTPNQPDVLVGGQSEPNDITEDTITDAPPT
                     VSGHNASTQPQSVVEDTAPQRPDVLIGGQSEPIDITQDTQPGMSGSNDATVINEDTKP
                     KRVFHFGNKEPQASEKATEQPAVPHDSHTTPQASDDTAAPHLPASGDKPEKVFALAAL
                     TMVAAAGLLSKKRRNTDKN"
BASE COUNT          701 a          529 c          425 g          437 t
ORIGIN      
        1 ttaaaaattt ctcattatcc ccctcaaaca ccggttcctc actgagatgg cgctttaaaa
       61 aggcgctagt ctcaaatcag gtatccccta cggttgaggg ggattaatgg gtatcacaac
      121 aagtaaaaag gtataatgat gaaatatgac taaaaaattt attaaatcag caatgattct
      181 aggcttagca agcataacca tcagcatgac gcaacccgtt gaagccattg caaatcgctt
      241 agaatatcaa gcacaatacg aacgtgtcaa agaagaacta gacgcattaa aagcaacaag
      301 tccaaaatta ggagaacgtt ccctttattt tagattggtg aatagatttc taggttggtt
      361 tggcgtagtc tgggacccac tcgcaatcaa agaaaccgat cacctgttgg acctcatcgg
      421 acaagtcaat tacttggtga gtaacaccga gtttcaagcc cctgaataca aacatgctaa
      481 aaacaaacta gacttagctg tagcaggcta tgagctctac ctagctagag aaaaagaacg
      541 tggtgatact cctgattacc aagtgaccca gctaacgttc aaacagctcg aaaccattaa
      601 aacgaagata cgagaggccc tggaaacatt tgcacaagag gtcaaaacaa tccaacaaaa
      661 aaacccagac ctatttcccc atgcttatgt tttaaacgct aagaccagtg ctatcgatgg
      721 taaagcgcaa ccctttttag tgaatggtta ctacgatgtc ttttataagc agtttttatt
      781 cactaataaa aatgacgggt ataactataa taaagaagca gacaacctaa ccttgtatca
      841 agatctgatt aaagagctat taggcttaaa agctgagatc gatcggttaa caaaagacaa
      901 gactgatgat agccgtttag agacgttaat gtgggaatca aagttcacct tatctgactt
      961 tcggagaact tcacgcctta gcgcctacta tacacaagaa gattatgtca aaaaggtggt
     1021 cctatctgaa gaagctccca acttgagaac agcaaaagaa aaggtcaaga ccttaaaagg
     1081 gatcttgcat gattactatg ttgacattaa agagccagaa aaagctaaac agtatcatgt
     1141 cgaggacgaa acctcaccgt ctcaacctga ggcccctgct cctggtcaaa aaccagcaga
     1201 agatgaggga tcgtcacaag ctacggagcc agctaacccg tcaaaagagg attcctcaac
     1261 ggacgcatct cagggatctc acgatagtga aaacccagcg acggataatc cttctcaacc
     1321 tcaggtacca gaccaaggca atcaccaaag ccaagtgcct aacgacaagc ctcaaacgga
     1381 caagacggac actcctaacg tcccagcctc tccacaggac actcctaacg ccccagcccc
     1441 tccacaggac accccaaaag tgcttgaggc tggtggtcag agtggccccg ctgggaacgc
     1501 tgaggagaaa gctcctgact ctggacctaa agagtctggt caaagctcat ctaaagagtc
     1561 cccatccgtt ggcgaagaca cgacccctaa tcagccagac gtccttgtag gtggtcaaag
     1621 tgagccaaac gacatcaccg aagacacaat cactgacgct cctccgacag tatctgggca
     1681 caacgcatca acacagcccc aatctgtggt cgaagacaca gcacctcagc gcccagatgt
     1741 ccttataggt ggtcaaagcg agccaatcga tatcacccaa gatacccaac cgggcatgtc
     1801 aggctctaat gacgctaccg ttatcaatga agatacaaaa ccaaaacgcg tcttccactt
     1861 tggtaacaaa gagccacaag catcagaaaa agcgacagaa caacctgccg ttcctcatga
     1921 tagccacaca acccctcaag cttcagatga tacagcagcc cctcacttgc ctgcatctgg
     1981 agacaagcct gaaaaagtgt ttgcacttgc tgccttgaca atggtcgcag ctgcagggct
     2041 actcagcaaa aaacgtcgca acaccgacaa aaactagtcg ctgttaggca ca
//
LOCUS       DAUCLR010000142         2086 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.145, whole genome shotgun sequence.
ACCESSION   DAUCLR010000142 DAUCLR010000000
VERSION     DAUCLR010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.145"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..489)
                     /locus_tag="VGT87_001770"
     CDS             complement(<1..489)
                     /locus_tag="VGT87_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEQ3814593.1"
                     /translation="MKKRILSAVLVSGVTLGAATVVSADNFDAKISDANSRISALTAE
                     QQSAQNQVNALQAQVSSLQAEQDKLTAKNSELEELSKGFEQEIQSLTNQIIARNEKLK
                     NQARSAYKNNETSGYINALLNSKSISDVITRLVAINKAVSANAKMLEQQKADKASLEE
                     KQA"
     gene            1236..1517
                     /locus_tag="VGT87_001771"
     CDS             1236..1517
                     /locus_tag="VGT87_001771"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEQ3814594.1"
                     /translation="MTVLNYVNTGDAKSIHNHLFHYTRQVRREVKVITIGILSAHYYE
                     ITMLFLQAKIILNCFHIPQRLRQAITRFRIRIINTSAIKPIAYRTLKLY"
     gene            complement(1992..2079)
                     /locus_tag="VGT87_001772"
     tRNA            complement(1992..2079)
                     /locus_tag="VGT87_001772"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2043..2045),aa:Ser,seq:gct)
BASE COUNT          724 a          380 c          258 g          724 t
ORIGIN      
        1 cgcttgtttt tcttcaagtg aagctttatc agctttttgt tgctctagca tcttagcatt
       61 agcagagact gctttgttaa tagcaactaa acgagtaatc acgtcagaaa ttgatttaga
      121 attcaaaagt gcattaatgt aaccagaagt ttcattgttt ttataagcac tacgagcttg
      181 attttttaat ttttcattac gagcaataat ttggtttgta agagattgaa tttcttgttc
      241 gaatcctttt gaaagttctt caagttctga attttttgct gttaatttat cttgttcagc
      301 ttgtaatgaa ctaacttgtg cctgtaatgc attaacttga ttctgagcag attgctgttc
      361 tgcagttaag gctgagattc ttgagttagc atcagagatt ttggcatcaa aattatccgc
      421 gctaactaca gtagctgctc caagggttac accacttaca agaacggctg atagaattct
      481 ttttttcata ataaaaaaac tactcctttt cgataagaca tcttatattt taccaaaaaa
      541 aaaagtattt aatattacgc aattgttaca gttatatgta ataaacaata aaaatatttt
      601 agggattatc ttataaaagt aaagcagcca aaaagatata tctttcattt aaaatagaat
      661 acagtaaaat caatttaaat acataaaatt gtataaaatt acacaaaaag tttgaaaatt
      721 atatcttaat aatgctataa gtaatacaaa attgtttatc ttcactcaga caccttctcc
      781 ttcttagaat gaatttttag ttacttatcc acaggttaat aacttctttt cgcctaaaat
      841 attatccaca atagtgatat tttctattat cagtttatgc ctttccattt tccaattaga
      901 atattagaca tcttttttga ccttatcatt attaatataa gtatagtcag accttgtttt
      961 tttataagct aactatcgaa aactggtcat ctataaatct tagtattcca tcactcaatc
     1021 acaattcata aagactcgca aactttcaca acttttaatt ctatactttc tgtagtaggt
     1081 aaatccacct caaagattat agagtctttt taaacatatg taaaaagttc catatgacga
     1141 tctataatga aaagcgacca aactatcatt tcagaaagac tcatatagaa taatccgatt
     1201 atagtataat ttcattagga atctaaaacc ttagcatcac ggttttaaat tatgtaaata
     1261 ctggggacgc aaaaagcatt cataatcatc tctttcatta taccagacag gttagaaggg
     1321 aggtcaaagt tattaccata ggcatactca gtgcccacta ctatgagatt actatgcttt
     1381 ttctacaggc taaaattatc ctcaattgtt ttcatatccc ccaacgtctt agacaagcta
     1441 taactcgctt tcgtatccga atcataaata cttctgctat aaaacctatc gcgtatagaa
     1501 ctctcaaact ttactgaaaa ctcatacagc atgattcacg gaagctcatc aataatagct
     1561 ttattgccca atttttcgta cgcccataac taacatctag aaaagaaaag ctcttactca
     1621 caagatctct gaatttcaat aattattcct aactctatca actcttgctc ttcccatttt
     1681 tcagacaatt tttaaaattt ttctaaaaga aaagaacaag attagcaata tacttaaact
     1741 caactactct aaggccaaat tagaaacagt caacaacctt atcaaagcca tcaagttaaa
     1801 cgttttactt ctctacagta gtcaaagagc ctatcatttt agttatcgaa ataatgaacg
     1861 atttaaatat tctatttagt tggttttgaa gattacaaaa aaaagacgat aaccgtcttc
     1921 tttccccttt atttataaat catccattaa tatgtttcat ttaacatatt aaccaaaata
     1981 tctttacaaa acggagaata agggattcga acccttgcgc cagttacccg acctaacgat
     2041 ttagcaaacc gtcctcttca gcctcttgag taattctcca aatata
//
LOCUS       DAUCLR010000143         1887 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.146, whole genome shotgun sequence.
ACCESSION   DAUCLR010000143 DAUCLR010000000
VERSION     DAUCLR010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.146"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..685
                     /gene="coaB"
                     /locus_tag="VGT87_001773"
     CDS             <1..685
                     /gene="coaB"
                     /locus_tag="VGT87_001773"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEQ3814595.1"
                     /translation="KLIITSGGTTEPIDAVRGITNHSTGQLGKLITERFLQYHHDVTL
                     VTTKTATKPLPNKRLRIIEVETVNDLMAALKDQVPHHDILIHSMAVSDYTPVYMTDLE
                     QVSQADNLNCFLCEHNSEPKISSASDYQVLFLKKTPKVISYVKQWNPNIKLVGFKLLV
                     NVPQEELIKVARASLAKNHADYILANDLVDIQTGMHKALLISNNEVASADTKEAIADL
                     LYERMTKHD"
     gene            678..1223
                     /gene="coaC"
                     /locus_tag="VGT87_001774"
     CDS             678..1223
                     /gene="coaC"
                     /locus_tag="VGT87_001774"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEQ3814596.1"
                     /translation="MTKHITLAVSGSISAYKAADLTSQLTKIGYDVHIIMTQAATQFI
                     TPLTLQVLSKNAIHLDVMDEHDPKVINHIELAKRTDLFIVAPASANTIAHLAYGFADN
                     LVTSVALALPATTPKLIAPAMNTKMYQNPITQENIKRLSTIGFTEIPPKSSLLACGDK
                     GPGALADIDVILATIDTIWKL"
     gene            1281..1850
                     /locus_tag="VGT87_001775"
     CDS             1281..1850
                     /locus_tag="VGT87_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEQ3814597.1"
                     /translation="MNHRKSADISRIAIFFAIMLVIHFVSSLVFNIWPIPIKPTLVHI
                     PVIIASVLYGPRIGAILGGLMGIISVITNTIILLPTNYLFSPFVDHGTFASLIIAIIP
                     RILIGITPYYCYKLIPNQFGLIVSGIIGSLTNTIFVLSGIFIFFATVFDGNIKALLTA
                     IISSNAIVEIIISAIITFVLIPTLSRLKR"
BASE COUNT          599 a          370 c          289 g          629 t
ORIGIN      
        1 gaaattaata ataacatcag gtggaacaac tgaacctata gatgcagtac gaggtatcac
       61 taatcactca actgggcaac ttggaaaatt aatcacagag cgatttttac aatatcatca
      121 tgacgttacc ctagtcacta ctaaaacagc cactaaaccc ctgccaaata agcgcctaag
      181 gattattgaa gtggaaacag ttaacgactt aatggctgca ctcaaagatc aggttcctca
      241 ccatgacatt ctaattcata gtatggctgt ttctgactat actccagtct acatgactga
      301 tttggaacaa gtctctcaag ctgataacct taattgtttt ctatgtgaac ataacagtga
      361 gccgaaaatc tcatctgcat ctgattatca ggttcttttt ttgaaaaaaa cacccaaagt
      421 tatctcttat gttaaacagt ggaatcctaa tattaaacta gtcggcttca aacttctagt
      481 taatgtgcct caagaagagt taattaaagt agccagagca agtctagcta aaaaccatgc
      541 tgattatatt ctcgccaatg atttagttga tatccaaaca ggtatgcaca aggctctttt
      601 gatatccaat aacgaagttg catctgcaga taccaaagag gcaattgctg acttattata
      661 tgaaaggatg accaaacatg actaaacata ttaccttagc tgtttctggt agtatatctg
      721 cctataaagc agcagatctt acgagtcaat taactaaaat tggctatgat gttcacatca
      781 taatgactca ggctgctaca caatttatta caccattaac cctgcaagtg ttatcaaaaa
      841 atgctattca tttagatgtt atggatgagc atgacccaaa agttatcaac catatcgaat
      901 tagcaaaacg cacagattta tttattgttg cacctgcctc tgctaatact attgctcatc
      961 tagcatatgg attcgctgat aatcttgtaa caagtgttgc tcttgcccta ccagctacca
     1021 caccaaaatt aatagcccca gccatgaata ccaaaatgta tcaaaaccct attactcaag
     1081 aaaatattaa acgcttatcg acaatagggt ttacagaaat tcctccaaaa tctagcttat
     1141 tagcctgtgg agataaagga cctggtgctt tggctgatat tgatgttatc ttagctacta
     1201 ttgatacgat ttggaaactt taacagtcgt ttttctgatc acctttttag aattaagtat
     1261 tatatttttt ggagagtaaa atgaatcacc gtaaatcagc agatatttct cgtattgcta
     1321 tcttttttgc catcatgtta gtcattcatt ttgtgagcag tcttgtcttt aatatttggc
     1381 ctattccaat caaaccaacg cttgtccata tcccagttat tattgcttct gtcttatatg
     1441 gcccaaggat aggtgctatt ttaggaggat taatggggat tataagtgtc attacaaata
     1501 ccatcattct actgcctaca aattatctct tttcaccttt tgtcgatcat ggaactttcg
     1561 cttctttaat aattgctatt atcccacgta ttttgattgg tattacccct tattattgct
     1621 ataaattgat tcctaatcag tttggtttaa tagtatccgg tatcattggt tctctaacaa
     1681 atactatatt tgtattatct gggatattta tttttttcgc gacagttttt gatggaaata
     1741 ttaaagcact actaacagcc atcatctcat caaacgctat tgttgagata atcatctcag
     1801 ctattattac ctttgtactt attccaacac tatcaagact aaaaaggtaa ttagctttca
     1861 caataaccga gtttttctct tttaaaa
//
LOCUS       DAUCLR010000144         1824 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.147, whole genome shotgun sequence.
ACCESSION   DAUCLR010000144 DAUCLR010000000
VERSION     DAUCLR010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.147"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..842
                     /locus_tag="VGT87_001776"
     CDS             <1..842
                     /locus_tag="VGT87_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="copper amine oxidase"
                     /protein_id="HEQ3814598.1"
                     /translation="TKKELEEKIKGLEGKQKASEEEIKKLKEELDKKIEEAKKLIEEA
                     NKKAKEELEKQAKDEKEKNINQGLSKKLDELLKLQKENKEKKEDKKSQDKKWDELLKA
                     DDKNILNQFDLNKMKKQEEQQGKKQVKDEKEFAVFQVDKNFYNIINKDGKTTVYMDVK
                     TYVDQGRTMIPVRYIAYTLGFNVEYDNSTREAIFSNKENNILAKKTLRLNIDTGVMKD
                     SDGKVYNSDVKPVIINGRIHASISNIAKAFGASHGDIKDGKNQTIEWDNARKAVYVFK
                     NVK"
     gene            857..1717
                     /locus_tag="VGT87_001777"
     CDS             857..1717
                     /locus_tag="VGT87_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HEQ3814599.1"
                     /translation="MNKSIKRGVAIALLLFTFTVPATTFAMTNEGQIESQNKSIYEPQ
                     KEEFEKMLKEDVFTPSKEEIPYQDVPRIPGNTSEANKPSNNPPKKTPLVKGGNTKAVN
                     NLATGENKARGSVIENVDRNGKDITPSGDTEKDKENPVDVRQFLTFQTKSGKTMHLIV
                     DHSSNQDNVRLLTEVGEQDLLNMIESEDKNTIKVEEPKKEEVKKEEPKTVPVKEEKKS
                     GIGSFLIVVLVIGGVVGAGYYFKVVKAKEDKMLEDFEEDDEDYISESEDESDNEESHE
                     EFLDEDDELL"
BASE COUNT          836 a          209 c          346 g          433 t
ORIGIN      
        1 atacaaaaaa agaattagaa gagaagatta aaggactaga aggaaaacaa aaagcttctg
       61 aagaagaaat taaaaagcta aaagaagagt tagataagaa aattgaagaa gccaagaagt
      121 taatcgagga agcaaataaa aaagctaaag aagaacttga aaagcaagct aaagatgaaa
      181 aagaaaaaaa tataaaccaa ggcttatcta agaaattaga tgaacttcta aaactccaaa
      241 aagaaaacaa agagaagaaa gaagataaga aatctcaaga taagaagtgg gacgaacttt
      301 tgaaagctga tgataagaac atcctaaatc aatttgatct taacaagatg aaaaagcaag
      361 aggaacaaca aggtaaaaaa caagttaaag atgaaaaaga atttgcagtt ttccaagtag
      421 acaaaaactt ctataacatt atcaacaaag atggtaagac aacagtctat atggacgtaa
      481 aaacttatgt agaccaagga agaactatga ttccagtaag atatattgca tatactctag
      541 gttttaatgt tgagtacgac aactctactc gtgaagctat tttctcaaat aaagagaata
      601 atatcctagc taaaaagaca ttaagactaa atattgatac tggtgttatg aaagattcag
      661 atggaaaggt ctacaattca gatgttaagc cagtgattat aaatggaaga attcatgctt
      721 caatttcaaa tattgctaaa gcttttggag caagtcatgg agatattaaa gatggcaaaa
      781 accaaacaat agaatgggac aatgctagaa aagcagtcta tgtatttaaa aatgtaaaat
      841 aagaaaggat agaaaaatga ataagagtat taaacgagga gtagccatag cgttactcct
      901 ttttacattt acagtaccag caactacatt tgcgatgaca aatgaaggac agattgaaag
      961 tcaaaacaaa tcaatctatg aaccacaaaa agaagaattt gaaaaaatgt tgaaagaaga
     1021 tgtttttaca ccatcaaaag aagaaattcc ttatcaagat gttccaagaa taccaggaaa
     1081 tacaagtgaa gcaaataagc cttcaaataa tcctcctaag aaaacaccac ttgtaaaagg
     1141 tggtaataca aaggcagtaa ataatcttgc gactggggaa aataaggcaa gaggttcagt
     1201 aattgaaaat gtcgatagga atggaaaaga tattacacca agtggagata cagaaaaaga
     1261 taaagaaaat ccagtagatg taagacaatt tttaacattt caaactaaaa gtggaaaaac
     1321 tatgcaccta atagtagatc actcatcaaa tcaagataat gtaagattat taacagaagt
     1381 aggagagcaa gacctactta atatgattga atcagaagat aaaaatacta taaaggttga
     1441 agagcctaag aaagaagaag taaaaaaaga agaacctaag actgttccag taaaagaaga
     1501 aaagaaaagt gggataggtt catttctaat tgttgtactt gtaattggag gagttgtagg
     1561 agcaggatat tattttaagg tagtcaaagc taaagaagat aaaatgttgg aagactttga
     1621 agaagatgat gaggattata ttagtgagtc agaagatgaa tctgacaatg aagaaagtca
     1681 tgaagaattt ttagatgaag atgatgaact attataagga tatgaaaatt taatctaaat
     1741 tgaatatttc tcccgaatat gatacaatat tcgggagaaa ggtggtgcaa aatgaattct
     1801 tatttagatc aaataaaaaa taga
//
LOCUS       DAUCLR010000145         1780 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.148, whole genome shotgun sequence.
ACCESSION   DAUCLR010000145 DAUCLR010000000
VERSION     DAUCLR010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.148"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..565)
                     /gene="rofA"
                     /locus_tag="VGT87_001778"
     CDS             complement(<1..565)
                     /gene="rofA"
                     /locus_tag="VGT87_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047149466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RofA"
                     /protein_id="HEQ3814600.1"
                     /translation="MIEKYLESSIESKCQLIILFFKTSYLPITEVAEKTGLTFLQLNH
                     YCEELNAFFPGSLSMTIQKRMISCQFTHPFKETYLYQLYASSNVLQLLAFLIKNGSHS
                     RPLTDFARSHFLSNSSAYRMREALIPLLRNFELKLSKNKIVGEEYRIRYLIALLYSKF
                     GIKVYDLTQQDKNTIHSFLSHSSTHLKT"
     gene            832..>1780
                     /locus_tag="VGT87_001779"
     CDS             832..>1780
                     /locus_tag="VGT87_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioester-forming surface-anchored protein"
                     /protein_id="HEQ3814601.1"
                     /translation="MNNKMFLNKEAGFLAHTKRKRRFAVTLVGVFFMLLVCAGAIGFG
                     QVAYAADEKTVPNRINSNPEFPWYGYDSYSGRLLRYHNLKVNLNGSKEYQAYCFNLKR
                     FEPKKEESSSPNWYKKLDGSTETFKKYAENPRFSGEELRRHILKVLYNGYPNSNEIMK
                     GIDPLNAILVTQNAIWYYSDSAPINDINNFFTSEANDLNIPPQQLTLMREALRKLISS
                     DENLVKQVPSNFKLSIFESSDKSYQNLLSAEYVPDDPPKPGDTSEHNPKTPELDGTPI
                     PEDPKRPDESSEPALPPLMPELDGEEVPEVPSESLEPALP"
BASE COUNT          605 a          285 c          351 g          539 t
ORIGIN      
        1 aggttttaag gtgggtggaa ctatgggata aaaagctatg aatagtgttt ttgtcttgct
       61 gcgtcaagtc ataaacttta atgccaaact tactatatag cagagcgatg aggtaacgga
      121 tgcgatattc ctcaccgaca atcttgttct tagagagttt taattcaaag tttcttaata
      181 aaggaatcaa tgcttcgcgc atccgataag ctgaggagtt tgataaaaaa tgacttcttg
      241 caaaatccgt aaggggacga gagtgggaac cattttttat taaaaacgct agtaattgta
      301 agacattaga tgatgcatag agttggtaaa gataagtttc tttaaaagga tgtgtaaatt
      361 ggcaagatat catccttttt tggatggtca tagacagact accagggaaa aaggcattca
      421 gttcctcaca ataatggttt agttgtaaaa aggttaagcc agttttttct gctacctcag
      481 ttattggcaa ataagatgtc ttaaaaaaca agataattaa ctgacatttt gattcgattg
      541 atgattccaa gtatttttct atcaaggaaa gggacctctc ttcctaaact tagaaagcca
      601 aataagtctt tgataagtgt attctcctat ttcaattcta tctaagctaa aaatagaaat
      661 caaatctaat ggtttttggt gaaaagtcga aaccttttga aatagcttta aagcaatatt
      721 ttctcaaaaa atcattttca aaaacattaa tttggtgaaa aatataatca aaaaattgtc
      781 tttcttgaca ataacgtggt aagctcatat atgtttttga gaggagagaa aatgaataac
      841 aaaatgtttt tgaataaaga agccggtttt ttggcacaca caaaaagaaa aaggcgattt
      901 gctgtcactt tagtgggagt cttttttatg cttttggtat gcgcgggtgc tatcggtttt
      961 ggtcaagtag cctatgctgc ggatgagaag actgtgccga atcgtattaa ttcaaatcct
     1021 gagtttcctt ggtatggtta tgattcgtat agcggaagat tgttaagata tcacaatctg
     1081 aaagtaaatc taaatggaag taaggagtat caagcgtatt gttttaacct gaaaaggttt
     1141 gagccaaaaa aagaagaaag tagcagtcct aattggtata aaaaattaga tgggagtaca
     1201 gaaactttta aaaaatacgc tgaaaaccct aggtttagtg gagaggaatt aagacgacat
     1261 atattgaagg ttctctacaa tggatatcct aatagtaatg aaataatgaa ggggatagac
     1321 cctctcaacg ctatcttagt aacacaaaat gccatatggt attactcgga tagtgcaccg
     1381 attaatgata ttaataactt ctttactagt gaagccaatg atcttaatat accacctcag
     1441 cagctaacgt taatgcgtga agctctgaga aaactaattt cctctgatga aaatttggtt
     1501 aaacaagttc catcgaactt taaacttagt atttttgagt cttctgataa gtcctaccaa
     1561 aatcttttaa gtgctgaata tgtacctgat gatcctccta aacctggtga tacgtcagaa
     1621 cataatccta aaactcccga gttggatggc actccaattc ccgaggaccc aaaacgtcca
     1681 gatgagagtt cagaacctgc gcttccccca ttaatgccag agctagatgg tgaagaagtc
     1741 ccagaagttc caagcgagag cttagaacct gcgcttcccc
//
LOCUS       DAUCLR010000146         1717 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.149, whole genome shotgun sequence.
ACCESSION   DAUCLR010000146 DAUCLR010000000
VERSION     DAUCLR010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.149"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..1703
                     /locus_tag="VGT87_001780"
     CDS             <1..1703
                     /locus_tag="VGT87_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002839222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEQ3814602.1"
                     /translation="KKKTYYKRKNYESDKFTRKKIDDLKNESKKVTTKYSKKAQDVKD
                     FISDKKIGELEKSKSKLKDNILKNKTKGSLSSGVLLSGAKSSELVRDYLSSGSDNTGV
                     ESGEKAANVSSKLQHGIRKYKLDKKKKSLKKLSKLDKKIRKRKSKLEFKNGLEDLKKS
                     DAYIKKNRFKKFYQRKQMKSMIAKKHETRLVDRVKKAILSLGKASKELIVRKSKMVLF
                     VVIGLGLMLSIMVGGGSVGMSGLSNSVNSVMTTTYLSQDTVLSEVNQEFSSMEYDLQS
                     QIESVKTSHPGYDEYIINKEGEIGHNTHELLSYITSRCGEVKSASEVKGILKELFDKM
                     YKLDFKEEIEIRTRTVARTRYDSRGNPYTSYEKEEYEYKKLIVTLKKKEMDEVVREIF
                     KDYPDNVVHYEALLEAKGNMGDVFGLGNGDLGEIVDNPNFGNPGLAFDSATAKALFNE
                     AEKHIGKRYVFGASGPSNFDCSGFVCWSFTKSGVKRMPRTTAWRIYKDYCNPVSPSEA
                     QPGDIIFFHSTYNSGTPISHVGIYAGNGMMIHAGDPIQYTSINSKYWKSHFYGFGRPR
                     "
BASE COUNT          704 a          209 c          345 g          459 t
ORIGIN      
        1 ataaaaagaa gacttactac aagagaaaaa attatgaaag tgataaattc actcgcaaga
       61 aaatcgatga cttaaaaaat gaatctaaga aagttacaac gaaatattct aagaaagcac
      121 aagatgttaa agactttatc tcagataaaa agattggaga gcttgaaaag tctaaaagta
      181 agctaaagga taatatctta aagaataaaa ctaaaggaag tctatcttct ggggttttat
      241 tatctggagc taagtcatca gagttagtga gagattatct aagttcagga tctgacaata
      301 ctggtgtgga atctggagaa aaggccgcta atgtaagctc taaactccag catggaataa
      361 ggaagtataa gctagacaaa aagaaaaagt ccttaaaaaa gctatctaaa cttgataaaa
      421 aaataagaaa gagaaaaagc aagttggagt ttaaaaatgg attagaagat ttaaaaaagt
      481 cagatgccta tataaagaaa aatagattta agaagtttta ccagagaaaa caaatgaaga
      541 gcatgatagc taaaaagcac gaaacaagac tggtagatag agttaaaaaa gctattttaa
      601 gtttaggtaa agcttctaaa gagctaatcg taagaaaaag taagatggtt ttatttgtgg
      661 taataggact aggtcttatg ctatcaatca tggttggtgg cggaagtgtt gggatgagtg
      721 gactgagtaa ctcagtgaac tcagtaatga caacaacata tctatcacaa gatacagttc
      781 taagtgaagt taatcaagaa ttttcatcaa tggagtatga cctacaatca caaatcgaaa
      841 gtgtaaaaac aagtcatcct ggatatgacg aatatattat aaacaaagaa ggagaaattg
      901 gtcataatac ccatgagctt ttatcctata taacttcaag atgtggagag gtaaaatcag
      961 cgtctgaagt aaaaggaatt ttaaaagaac ttttcgataa gatgtataaa cttgatttta
     1021 aagaagaaat tgaaattaga acaagaacag tagcaagaac tagatatgat agtcgtggca
     1081 atccttatac tagctatgaa aaagaagagt acgaatataa aaagttaatt gtaactttaa
     1141 agaaaaaaga aatggatgaa gttgttaggg aaatatttaa agattatcca gacaatgtag
     1201 ttcattatga agctcttctt gaagctaagg gaaatatggg agatgttttt ggattaggta
     1261 atggggactt aggagaaata gtagataatc caaactttgg aaatcctggt cttgcttttg
     1321 attcagctac tgcaaaagcc ttatttaatg aagcagaaaa acatataggt aaaagatatg
     1381 tgtttggagc aagtggacca tctaactttg actgctcagg ttttgtatgt tggtcattta
     1441 caaagtctgg tgtaaagaga atgccaagaa caactgcatg gagaatatat aaagactatt
     1501 gtaatccagt aagtccaagt gaagctcaac ctggagatat tatatttttc cactcaacat
     1561 ataacagtgg aacaccaata tctcacgtag gaatatatgc aggtaacgga atgatgattc
     1621 acgcaggaga tccaattcaa tacacatcaa taaattcaaa atattggaaa tcacactttt
     1681 atggttttgg aagaccaaga tagaagggag atattat
//
LOCUS       DAUCLR010000147         1685 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.150, whole genome shotgun sequence.
ACCESSION   DAUCLR010000147 DAUCLR010000000
VERSION     DAUCLR010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.150"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..276
                     /gene="murD"
                     /locus_tag="VGT87_001781"
     CDS             <1..276
                     /gene="murD"
                     /locus_tag="VGT87_001781"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054713.1"
                     /note="UDP-N-acetylmuramoylalanine--D-glutamate ligase;
                     involved in peptidoglycan biosynthesis; cytoplasmic;
                     catalyzes the addition of glutamate to the nucleotide
                     precursor UDP-N-acetylmuramoyl-L-alanine during cell wall
                     formation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEQ3814603.1"
                     /translation="FDDLVPDLLGLKQMIILGESAERMKRAANKAEVSYLEARNVAEA
                     TELAFKLAQTGDTILLSPANASWDMYPNFEVRGDEFLATFDCLRGDA"
     gene            276..1358
                     /locus_tag="VGT87_001782"
     CDS             276..1358
                     /locus_tag="VGT87_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEQ3814604.1"
                     /translation="MPKKILFTGGGTVGHVTLNLILIPKFIKDGWEVHYIGDKNGIEH
                     TEIEKSGLDVTFHAIATGKLRRYFSWQNLADVFKVALGLLQSLFIVAKLRPQALFSKG
                     GFVSVPPVVAAKLLGKPVFIHESDRSMGLANKIAYKFATTMYTTFEQEDQLSKVKHLG
                     AVTKVFKDANQMPESTQLEAVKEYFSRDLKTLLFIGGSAGAHVFNQFISDHPELKQRY
                     NIINITGDPHLNELSSHLYRVDYVTDLYQPLMAMADLVVTRGGSNTLFELLAMAKLHL
                     IVPLGKEASRGDQLENATYFEKRGYAKQLQEPDLTLHNFDQAMADLFEHQADYEATML
                     ATKEIQSPDFFYDLLRADISFAIKEK"
     gene            1359..>1685
                     /locus_tag="VGT87_001783"
     CDS             1359..>1685
                     /locus_tag="VGT87_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEQ3814605.1"
                     /translation="MAKDKEKQSDDKLVLTEWQKRNIEFLKKKKQQAEEEKKLKEKLL
                     SDKKAQQQAQNASEAVELKTDEKTDSQEIESETTTKPKKPKKVRQPKEKSATQIAFQK
                     SLPVLLG"
BASE COUNT          528 a          310 c          377 g          470 t
ORIGIN      
        1 tttgacgatt tggtgccaga ccttttagga cttaagcaga tgattatttt gggagaatcc
       61 gcagagcgta tgaagcgagc tgctaacaaa gcagaggtct cttatcttga agctagaaat
      121 gtggcagaag caacagagct tgcttttaag ctggcccaaa caggcgatac tatcttgctt
      181 agcccagcca atgctagctg ggatatgtat cctaattttg aggttcgtgg ggatgaattt
      241 ttggcaacct ttgattgttt aagaggagat gcctaatgcc taagaagatt ttatttacag
      301 gtggtggaac tgtaggtcat gtcaccttga acctcattct cataccaaaa tttatcaagg
      361 acggttggga agtacattat attggtgata aaaatggcat tgaacataca gaaattgaaa
      421 agtcaggcct tgacgtgacc tttcatgcta tcgcgacagg caagcttaga cgctattttt
      481 catggcaaaa tctagctgat gtttttaagg ttgcacttgg cctcctacag tctctcttta
      541 ttgttgccaa gcttcgccct caagcccttt tttccaaagg tggttttgtc tcagtaccgc
      601 cagttgtggc tgctaaattg cttggtaaac cagtctttat tcatgaatca gatcggtcaa
      661 tgggactagc aaacaagatt gcctacaaat ttgcaactac catgtatacc acttttgagc
      721 aggaagacca gttgtctaaa gttaaacacc ttggagcggt gacaaaggtt ttcaaagatg
      781 ccaaccaaat gcctgaatca actcagttag aggcggtgaa agagtatttt agtagagacc
      841 taaaaaccct cttgtttatt ggtggttcgg caggggcgca tgtgtttaat cagtttatta
      901 gtgatcatcc agaattgaag caacgttata atatcatcaa tattacagga gaccctcacc
      961 ttaatgaatt gagttctcat ctgtatcgag tagattatgt taccgatctc taccaacctt
     1021 tgatggcgat ggctgacctt gtagtgacaa gagggggctc taatacactt tttgagctac
     1081 tggcaatggc taagctacac ctcatcgttc ctcttggtaa agaagctagc cgtggcgatc
     1141 agttagaaaa tgccacttat tttgagaaga ggggctacgc taaacaatta caggaacctg
     1201 atttaacttt gcataatttt gatcaggcaa tggctgattt gtttgaacat caggctgatt
     1261 atgaggctac tatgttggca actaaggaga ttcagtcacc ggacttcttt tatgaccttt
     1321 tgagagctga tattagtttc gcgattaagg agaagtaaat ggcaaaagat aaagagaaac
     1381 aaagtgatga caagctcgtt ttgacagagt ggcaaaagcg taacattgaa tttttaaaga
     1441 aaaagaagca gcaagctgag gaagaaaaaa aactcaaaga gaaattattg agtgataaaa
     1501 aagcgcagca gcaagctcaa aatgcttctg aagcagttga gcttaaaact gatgagaaaa
     1561 ctgatagtca ggaaattgag tcagaaacga cgacaaaacc taaaaaaccc aaaaaagtta
     1621 gacaacccaa ggaaaaaagc gcgacacaaa tcgcttttca aaaatccttg cctgttcttt
     1681 tgggg
//
LOCUS       DAUCLR010000148         1663 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.151, whole genome shotgun sequence.
ACCESSION   DAUCLR010000148 DAUCLR010000000
VERSION     DAUCLR010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.151"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            79..>1663
                     /gene="lepA"
                     /locus_tag="VGT87_001784"
     CDS             79..>1663
                     /gene="lepA"
                     /locus_tag="VGT87_001784"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEQ3814606.1"
                     /translation="MNSQDLKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRHEVEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVDAPLQALIFDSVYDAYRGVILQ
                     VRIVNGIVKPGDKIQMMSNGKTFDVTEVGIFTPKAVGRDFLATGDVGYVAASIKTVAD
                     TRVGDTVTLANNPAKEALHGYKQMNPMVFAGIYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVVYHVHTTDEDMIE
                     VSNPSEFPDPTRVAFIEEPYVKAQIMVPQEFVGAVMELSQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYDMSEYRRSQLVKMDILLNGDKVDALSFI
                     VHKEFAYERGKIIVDKLKKI"
BASE COUNT          466 a          306 c          405 g          486 t
ORIGIN      
        1 aatccgtaag aaaagatagg caaatgccta ccttttttgt tataatagaa ggaaagacta
       61 aatgtatagg aaaatagaat gaacagtcaa gatttaaaaa aacgtcagga gaagattcgt
      121 aatttctcca ttatcgcaca tattgaccat ggcaaatcta ctctcgctga ccgcattttg
      181 gaaaagacag agacggtttc gtctcgtgaa atgcaggctc agttattgga ctccatggat
      241 ttggagcgtg agcgtgggat taccatcaag ttaaacgcca ttgagcttaa ttacacggct
      301 aaagatgggg aaacctacat tttccacctt attgataccc cagggcatgt ggactttact
      361 tatgaagtat cgcgttcgtt ggcagcttgt gagggagcga ttctagttgt ggatgcagcg
      421 cagggtattg aagcacagac ccttgccaat gtttacctag cccttgataa tgatttggaa
      481 attttaccgg ttatcaataa aattgatttg cctgctgctg accctgagag agtccgccat
      541 gaagtagaag atgtcattgg acttgatgct tctgaggctg ttctggcatc agccaaggct
      601 ggtattggga tcgaagagat tcttgagcaa attgttgaga aggttcctgc tcctacgggt
      661 gatgtggatg cgcctttaca ggccttgatt tttgactctg tgtatgatgc ttaccgaggc
      721 gttatcctgc aagttcggat tgtgaacggt attgtcaagc ctggcgataa aatccagatg
      781 atgtcgaatg gaaaaacttt tgatgttaca gaagttggca ttttcactcc taaggcagtt
      841 ggacgagatt tccttgcaac gggagatgtt ggttatgttg cggcttctat caaaacggta
      901 gcagataccc gtgtggggga tacggtgact ttagctaata accctgctaa ggaagccttg
      961 catggctaca agcagatgaa cccgatggtc tttgcaggga tttatccgat tgaatctaat
     1021 aaatacaatg acttgcgtga agcgcttgaa aaattacaac tgaacgatgc cagtttacag
     1081 tttgaacccg aaacgtcaca agcgcttggc tttggttttc gatgcggctt tttaggcttg
     1141 cttcacatgg atgtgattca agaacgtttg gaacgtgaat ttaacattga tttgatcatg
     1201 acagcaccgt ccgtggtgta tcacgttcac acaacagatg aggacatgat tgaagtgtct
     1261 aacccttcag aatttccaga cccaacccgg gtggctttta ttgaagagcc ttatgttaaa
     1321 gcgcaaatca tggtgccaca agagttcgta ggggctgtta tggaattatc gcagcgcaag
     1381 cgtggtgatt ttgtgaccat ggattacatt gatgataacc gtgtcaatgt gatttaccaa
     1441 atcccgctgg ctgaaattgt ctttgatttc tttgataaat tgaaatcctc aacgcgaggc
     1501 tatgcgagtt ttgattatga catgtcagaa taccgcaggt cacaattggt gaaaatggac
     1561 attcttttga acggtgataa ggtcgatgcg ctcagcttta ttgttcataa agaatttgct
     1621 tatgaacgcg ggaaaattat tgttgataaa ttgaaaaaaa tca
//
LOCUS       DAUCLR010000149         1642 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.152, whole genome shotgun sequence.
ACCESSION   DAUCLR010000149 DAUCLR010000000
VERSION     DAUCLR010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.152"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..199
                     /locus_tag="VGT87_001785"
     CDS             <1..199
                     /locus_tag="VGT87_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814607.1"
                     /translation="IEEKLKDLNAKYKEICDEEIQVENEEIIVTLRRNNISLEELMEK
                     INDRKREEKLKEKENIHNEEI"
     gene            186..>1642
                     /locus_tag="VGT87_001786"
     CDS             186..>1642
                     /locus_tag="VGT87_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019034346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HEQ3814608.1"
                     /translation="MKKFNTNKLRAFFMALLLVLTSTVHTSAFAKSDVTWTEDDFMYS
                     SEGNIIAFSDKGLEKKEKTNILVFPEGTKSINGNYSLSHSNDELRYKREFGRGKHWDK
                     VIIPDSVKHLGYAAFYEASIDEVKLSNSLTYLGGLAFFNCGLREVTLPNTLVNIEHNA
                     FERNNLQEITIPKSVKTIEQYAFSRNKLHTVKILGNPNINSKGVFHNQEVEYRPKQNP
                     FYENHFGFNGNQGFKSIPNGLRYENGEFVFDKNVDSVELEFDYNNDMYQGKMTIYNPN
                     KYSIGTQTDLTGQDIDEKDNKIDDLTKNIKDLENQIKDLNDKKQEDQSKIDELKEKLE
                     SCKDNGEKLKQEKARLEEEIRDKDNKIAQLNKEIEDLKNSNNDELIAEITQLKDELKR
                     LQDENAKLKEDYSSTKWELEAEKEKTDKNENKIKEMQEKLESLEGELAKKTKEIEDKD
                     NKIKGLEKALDEKDNKIKDLESKKKETENSKSECC"
BASE COUNT          732 a          176 c          299 g          435 t
ORIGIN      
        1 aatagaagag aagttaaaag atttaaatgc aaaatacaag gaaatttgcg acgaagaaat
       61 acaagttgaa aatgaagaaa taattgtaac tcttaggagg aacaatatta gcttggaaga
      121 attaatggag aaaatcaatg atagaaaaag agaagaaaaa ttaaaagaaa aggagaatat
      181 tcataatgaa gaaatttaat acgaacaaac taagagcctt ttttatggct cttttattag
      241 ttttaacatc aactgtgcat acaagtgcat ttgcaaagtc tgatgttact tggacggaag
      301 atgattttat gtattcctct gagggaaata taattgcctt cagtgataaa ggcttagaaa
      361 agaaagaaaa gacaaatatt ctagtatttc ctgaaggaac gaagtctata aatggaaatt
      421 attctttaag tcattcaaac gatgaactta gatataaaag agaatttggg cgtggcaaac
      481 attgggataa agtaattatt cctgattctg ttaagcattt aggttatgct gccttttacg
      541 aagcaagtat agatgaagta aaactatcaa atagtctaac ttatcttggt ggtttagcat
      601 ttttcaattg tggacttaga gaagtaactt tacctaatac tttggtaaat attgaacata
      661 atgcttttga aagaaataac cttcaagaaa taactatacc gaagtctgtg aagacaattg
      721 aacagtatgc tttttcaaga aataaactgc acactgtaaa aattttaggc aatccaaaca
      781 ttaacagtaa gggagtattc cataatcaag aagttgagta tagaccaaaa caaaatccat
      841 tttacgaaaa tcattttggt ttcaacggaa atcaaggatt caaatctatt cctaatggat
      901 taaggtatga aaatggagag tttgtctttg ataagaatgt agatagtgta gaacttgaat
      961 ttgattataa caatgatatg tatcaaggaa agatgactat atacaatcca aataaatatt
     1021 ccattggtac tcaaactgat ttgacaggac aagatattga tgaaaaggac aataagattg
     1081 atgatttaac taaaaacatt aaggacttag aaaatcagat caaagactta aatgataaga
     1141 aacaagaaga tcaatcaaag atagatgagt taaaggaaaa gttagaatct tgcaaagata
     1201 atggagagaa actaaaacaa gaaaaagcta ggctagaaga agaaattaga gataaagaca
     1261 ataagattgc tcaattgaat aaagaaattg aggatcttaa aaactccaat aatgatgaac
     1321 taatagcaga aattactcag cttaaagatg aattaaaaag attacaagat gaaaatgcaa
     1381 aactaaaaga agattattca tctacaaaat gggagttaga agctgagaaa gaaaagacag
     1441 ataaaaacga aaataaaatc aaagaaatgc aagaaaagct tgagtcttta gaaggagaac
     1501 ttgcaaaaaa gactaaagaa attgaggata aagataataa aattaagggc ttagaaaaag
     1561 ctcttgatga aaaagataat aagataaaag atttagagtc taaaaagaaa gaaacagaaa
     1621 attctaagtc agaatgctgt aa
//
LOCUS       DAUCLR010000150         1538 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.153, whole genome shotgun sequence.
ACCESSION   DAUCLR010000150 DAUCLR010000000
VERSION     DAUCLR010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.153"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            298..1494
                     /gene="tuf"
                     /locus_tag="VGT87_001787"
     CDS             298..1494
                     /gene="tuf"
                     /locus_tag="VGT87_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEQ3814609.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETATRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDTKFEDIIMELMDTVDSYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGTVRVNDEIEIVGIKEETKKAVVTGVEMFRKQLDEGLAG
                     DNVGILLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILSKDEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTINVELIHPIAVEQGTTFSIREGGRTVGSGI
                     VSEIEA"
BASE COUNT          482 a          329 c          305 g          422 t
ORIGIN      
        1 tcaaaaaact tgaactgctc tgctgatttc ttatttttgc gtgtgacatg agcccaaaca
       61 gctgcttttt gctgtggcaa aaataaggcg tggaattcca aaggaagtcg ctgctataag
      121 caccacctaa ggagagtatc gaaaaagact ttttcttcta tttaaaaaat aaagaaatgg
      181 caaaaaacat aaaatacttg cattttcatt gtaatttgct acaatagtaa ggtaaagtta
      241 gactgtatgg cctaccgtct atctataaaa tatattttat tggaggcttt tcctaaaatg
      301 gcaaaagaaa aatacgatcg tagtaaaccc cacgttaaca ttggtacaat cggacacgtt
      361 gaccatggta aaactacttt aacagctgca atcacaactg tattggcacg tcgcttgcct
      421 tcatcagtta accaaccaaa agattatgct tctatcgatg ctgctccaga agaacgcgaa
      481 cgcggaatca ctatcaacac tgcacacgtt gagtacgaaa ctgcaactcg tcactatgcg
      541 cacatcgacg ctccaggaca cgcggactac gttaaaaaca tgatcactgg tgccgctcaa
      601 atggacggag ctatccttgt agttgcttca acagatggac caatgccaca aactcgtgag
      661 cacatccttc tttcacgtca ggttggtgtt aaacacctta tcgtgttcat gaacaaagtt
      721 gaccttgttg atgacgaaga gttgcttgaa ttagttgaga tggaaattcg tgaccttctt
      781 tcagaatacg atttcccagg tgatgacctt ccagttatcc aaggttcagc tcttaaagct
      841 cttgaaggcg acactaaatt tgaagacatc atcatggaat tgatggatac tgttgattca
      901 tacattccag aaccagaacg cgacactgac aaaccattgc ttcttccagt cgaagacgta
      961 ttctcaatta caggtcgtgg tacagttgct tcaggacgta tcgaccgtgg tactgttcgt
     1021 gtcaacgacg aaatcgaaat cgttggtatc aaagaagaaa ctaaaaaagc tgttgttact
     1081 ggtgttgaaa tgttccgtaa acaacttgac gaaggtcttg caggagacaa cgtaggtatc
     1141 cttcttcgtg gtgttcaacg tgacgaaatc gaacgtggtc aagttattgc taaaccaggt
     1201 tcaatcaacc cacacactaa attcaaaggt gaagtatata tcctttctaa agacgaaggt
     1261 ggacgtcaca ctccattctt caacaactac cgtccacaat tctacttccg tacaactgac
     1321 gtaacaggtt caatcgaact tccagcaggt acagaaatgg ttatgcctgg tgataacgtg
     1381 acaatcaacg ttgagttgat ccacccaatt gccgtagaac aaggtactac tttctcaatc
     1441 cgtgaaggtg gacgtactgt tggttcaggt atcgtttcag aaatcgaagc ttaattttaa
     1501 ttaagttccc agaatagcaa tttaagtcag acaactta
//
LOCUS       DAUCLR010000151         1537 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.154, whole genome shotgun sequence.
ACCESSION   DAUCLR010000151 DAUCLR010000000
VERSION     DAUCLR010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.154"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            137..1342
                     /gene="rpsA"
                     /locus_tag="VGT87_001788"
     CDS             137..1342
                     /gene="rpsA"
                     /locus_tag="VGT87_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEQ3814610.1"
                     /translation="MNEFEDLLNSVSEVNPGDVVTAEVLTVDNGQANVVIEGTGVEGV
                     LTLRELTNDRDADINDFVKAGDTVEVLVLRQVVGKDTDTVTFLVSKKRLEARKAWDKL
                     VGREGEVVTVKGTRAVKGGLSVEFEGLRGFIPASMIDTRFVRNTEKFVGQEFDAKIKE
                     VDAAENRFILSRREVIEEAAKEARAEVFSKISEGAVVTGTVARLTSFGAFIDLGGVDG
                     LVHVTELSHERNVSPKSVVSVGEEVEVKVLSIDEEAGRVSLSLKATTPGPWDGVEQKL
                     AQGDVVEGKVKRLTDFGAFVEVLPGIDGLVHISQISHKRVENPKDVLSVGQEVTVKVL
                     EVNAADERVSLSIKALEERPAQAEGDNKEEKRQSRPRRPKRESRRDYELPETQTGFSM
                     ADLFGDIEL"
     gene            1455..1526
                     /locus_tag="VGT87_001789"
     tRNA            1455..1526
                     /locus_tag="VGT87_001789"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1488..1490,aa:Arg,seq:ccg)
BASE COUNT          452 a          261 c          358 g          466 t
ORIGIN      
        1 gctaccccag tcagaggtat caggtgctcc ctgagaccgt caaagataag agttgttgtc
       61 gttgcgggtt accttgagaa aatatattgt tatcaaaagc tagcattggc tagcttttgt
      121 tggaggattt tttagaatga atgaatttga agatttgcta aacagtgtta gcgaagtgaa
      181 ccctggtgat gttgtcactg cggaagtttt aactgttgat aacggtcaag caaacgttgt
      241 tatcgaagga acaggtgttg aaggtgtctt gacacttcgt gaattgacta acgaccgcga
      301 tgctgatatt aacgactttg ttaaagctgg cgacacagtt gaagtacttg ttcttcgtca
      361 agtagtaggt aaagatactg atacagttac tttcctagta tctaaaaaac gtttggaagc
      421 tcgtaaagcc tgggacaaac ttgttggtcg tgaaggcgaa gttgttactg ttaaaggtac
      481 ccgtgctgtt aaaggtggtc tttcagttga atttgaagga cttcgtggat ttatccctgc
      541 ttcaatgatt gacactcgtt ttgttcgcaa cactgaaaaa tttgttggac aagagtttga
      601 tgctaaaatc aaagaagttg atgcagcaga aaaccgtttc atcctatcac gtcgtgaagt
      661 tatcgaagag gccgctaaag aagctcgtgc tgaagtgttc tctaagattt cagaaggtgc
      721 agtcgttaca ggtactgtag cgcgtttaac aagttttggt gctttcattg accttggtgg
      781 tgttgacgga cttgttcacg tgactgaatt gtctcacgaa cgtaacgtgt cacctaaatc
      841 agttgtttca gttggtgaag aagttgaagt taaagttctt tcaattgacg aagaagctgg
      901 tcgtgtgtca ctttcactta aagcaacaac acctggacca tgggacggcg ttgaacaaaa
      961 acttgctcaa ggtgatgttg ttgaaggtaa agtaaaacgc ttgactgact tcggtgcttt
     1021 tgttgaagta ttaccaggta ttgatggact tgttcatatt tcacaaattt cacacaaacg
     1081 tgttgaaaat ccaaaagatg tactttctgt aggacaagaa gtaacagtta aagttcttga
     1141 agtgaatgca gctgatgagc gcgtatcatt atcaatcaaa gctcttgaag aacgcccagc
     1201 gcaagctgaa ggagacaaca aagaagaaaa acgtcaatca cgcccacgtc gtccaaaacg
     1261 tgaatcaaga cgtgactacg aactcccaga aacacaaact ggattctcaa tggctgatct
     1321 tttcggtgat attgaattgt aataaatcta aaaagttgag gttttcctca actttttatt
     1381 gtcttctggt tagaaaagtt atttcatatt atttcatttt atgatatact ataagggtat
     1441 tgacaaagtt gtgaccacct ttagtgtaat ggatatcacg taagattccg gttcttgaga
     1501 tgggggttcg attccctcaa ggtggataga aatcata
//
LOCUS       DAUCLR010000152         1530 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.155, whole genome shotgun sequence.
ACCESSION   DAUCLR010000152 DAUCLR010000000
VERSION     DAUCLR010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.155"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..734
                     /locus_tag="VGT87_001790"
     CDS             <1..734
                     /locus_tag="VGT87_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HEQ3814611.1"
                     /translation="ALKTFDYSSVETVVRVNGLDSCGVLDIEAVVLAGVNVIRLPKTE
                     TAQDIVDVEAVIERVERENGIEVGRTRMMAAIESAEGVLNAREIAKASKRLIGIALGA
                     EDYVTNMKTRRYPDGQELFFARSMILHAARAAGIAAIDTVYSDVNNTEGFQNEVRMIK
                     QLGFDGKSVINPRQIPLVNEIYTPTEKEIDHAKQVIWAIREAESKGSGVISLNGKMVD
                     KPIVERAERVIALATAAGVLSEEDI"
     gene            737..>1530
                     /locus_tag="VGT87_001791"
     CDS             737..>1530
                     /locus_tag="VGT87_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407592.1"
                     /note="citrate-ACP transferase, the alpha subunit
                     catalyzes the formation of (3S)-citryl-CoA from acetyl-CoA
                     and citrate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HEQ3814612.1"
                     /translation="MVTNKLGRDIPQPYADQYGVFEGELANIKQYDESSRRIKPVKPG
                     DSKLLGSVREAIEKTGLTDGMTISFHHHFREGDFIMNIVLEEIAKMGIKNLSIAPSSI
                     ANVHEPLIDHIKNGVVTNITSSGLRDKVGAAISEGLMENPVVIRSHGGRARAIASGDI
                     HIDVAFLGAPSSDAYGNVNGTKGKATCGSLGYAMIDAKYAEQVVILTDNLVPYPNTPT
                     SIPQTDVDYVVTVDAIGDPQGIAKGATRFTKNPKELLIAEYAAKVIT"
BASE COUNT          446 a          305 c          351 g          428 t
ORIGIN      
        1 ttgcgcttaa aacttttgat tattcaagcg ttgaaacggt tgttcgtgtg aatggtcttg
       61 attcttgtgg ggttttagac atcgaagctg ttgttttggc aggtgtcaac gtgattcgtc
      121 ttccaaaaac agagactgcc caagatattg ttgatgtgga ggctgttatt gaacgtgtcg
      181 aacgcgaaaa cggcattgaa gttggtcgca cacgcatgat ggcagccatt gaatcagccg
      241 aaggtgtcct aaatgctcgt gagattgcca aagcttctaa gcgcttgatt ggtattgctc
      301 ttggggcaga agactatgtc acgaatatga aaacgcgtcg ttacccagat ggtcaagaat
      361 tattctttgc tcgtagcatg attttacatg ctgctcgtgc tgctggaatt gctgccattg
      421 atactgttta ttctgatgtc aataatacag aaggattcca aaacgaagtt cgtatgatca
      481 aacagttagg atttgacggt aaatcggtca tcaaccctcg tcaaattcct ctggtcaatg
      541 agatttatac cccaaccgaa aaagaaattg accatgccaa acaagtcatc tgggcaattc
      601 gtgaagctga aagcaaaggc tctggcgtta tttccttaaa tggaaaaatg gttgataaac
      661 caatcgttga acgtgcagaa cgtgtcattg ccctagcaac agcagcaggt gttttatctg
      721 aggaggacat ttaaagatgg taacaaataa actcggtaga gatattcctc aaccatacgc
      781 cgaccaatac ggtgtctttg aaggagaact cgcaaatatc aaacagtatg acgaatcaag
      841 tcgccgtatt aaaccagtta agcctggaga ttctaaatta ctaggatctg ttcgtgaagc
      901 gattgaaaaa acaggtttaa cagatggcat gaccatttct ttccaccacc atttccgtga
      961 aggggatttc attatgaata tagtcttgga ggaaattgcc aaaatgggca ttaaaaacct
     1021 gtctattgcc ccaagttcca tcgctaatgt acatgagcct ttgattgatc acatcaaaaa
     1081 tggtgtggtc actaacatca cttcatctgg tcttcgtgac aaagtagggg cagccatttc
     1141 agaaggacta atggaaaatc ctgtggttat tcgctcccat ggtggtcgcg ctcgtgccat
     1201 tgctagtggg gatatccata ttgatgttgc ctttctgggc gccccaagtt cagatgctta
     1261 cggaaatgtt aatgggacaa aagggaaagc aacctgtggt tctttgggct atgccatgat
     1321 tgatgccaaa tatgcggaac aggtggttat cttgacagat aatttggttc cttatcctaa
     1381 taccccaacc agcattcctc aaacagatgt tgactatgtg gtaacagtgg atgctatcgg
     1441 agatcctcaa ggaatcgcca aaggggcaac tcgttttacg aaaaatccta aagaactctt
     1501 aattgcagaa tacgccgcta aagtaatcac
//
LOCUS       DAUCLR010000153         1521 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.156, whole genome shotgun sequence.
ACCESSION   DAUCLR010000153 DAUCLR010000000
VERSION     DAUCLR010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.156"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            16..1038
                     /locus_tag="VGT87_001792"
     CDS             16..1038
                     /locus_tag="VGT87_001792"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEQ3814613.1"
                     /translation="MTIAIDWDNLGFEYHKLPFRYISYYKNGQWDKGQLTEDATLHIS
                     ESAPALHYGQQAFEGLKAYRTKDGSIQLFRPDRNAVRLQATADRLLMPQVSTEQFIDA
                     AKQVVKANEDFVPPYGTGATLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LAPTNFIVSEAFDRAAPYGTGAAKVGGNYAGSLLPGKAAKSAGFSDVIYLDPATHTKI
                     EEVGAANFFGITANNEFVTPLSPSILPSITKYSLLQLAEERLGMTVIEGDVPINELDK
                     FVEAGACGTAAVISPIGGIQYKDNLHVFYSETEVGPVTRRLYDELVGIQFGDIEAPEG
                     WIVKVD"
     gene            1102..1332
                     /locus_tag="VGT87_001793"
     CDS             1102..1332
                     /locus_tag="VGT87_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEQ3814614.1"
                     /translation="MSKKDKKIEIQLIDHKVMVNETKIDGYQLQIGKRVVGEIAELDE
                     KFAVLKNDGVDCFFKTLEQAVENIIENYNLNH"
     gene            1393..1473
                     /locus_tag="VGT87_001794"
     tRNA            1393..1473
                     /locus_tag="VGT87_001794"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1427..1429,aa:Tyr,seq:gta)
BASE COUNT          494 a          261 c          327 g          439 t
ORIGIN      
        1 aaaagaggta cgatgatgac aatagcaatt gattgggaca acctaggatt tgaatatcat
       61 aagttgccat ttcgttacat ttcttattac aaaaatggtc aatgggataa aggtcaatta
      121 acagaagatg ccaccttaca tatctcagaa agcgctccag cgcttcatta tgggcaacaa
      181 gcttttgaag gtttaaaggc ttatcgcacc aaagatggct ctattcaact atttagacca
      241 gaccgtaatg cagtgcgttt acaagccaca gcagaccgct tgttgatgcc acaagtatca
      301 acagagcaat ttattgatgc agctaagcaa gtggttaaag ccaatgaaga ctttgtacca
      361 ccttacggaa caggtgcaac gctttacctg agaccgttat taattggagt tggagatatt
      421 attggggtta aaccagccga agaatatatt ttcaccattt ttgccatgcc agtaggaaac
      481 tatttcaaag gtggacttgc tccaactaat tttattgttt ctgaagcgtt tgaccgcgcc
      541 gctccttacg gaacaggtgc tgctaaggtg ggaggaaact atgcaggaag tttgctacca
      601 ggtaaggccg ctaaatcagc aggcttttct gatgtgattt acttagatcc agcaacccat
      661 actaaaattg aagaagtcgg agctgcaaac ttttttggca ttactgcaaa taatgaattt
      721 gttacgccac ttagtccatc catcttgcca tctattacga agtattcact cttacaacta
      781 gctgaagaaa gacttggtat gacagtaatt gagggtgatg ttccaattaa tgaattggat
      841 aaatttgtgg aagcaggagc ttgtggaaca gcagctgtta tctctccaat tggcggtatc
      901 cagtacaagg ataacttaca tgtcttttac agtgaaacag aagttggtcc tgtgacacgc
      961 cgtttatacg atgagttggt cggcattcaa tttggggata ttgaagcacc agaaggttgg
     1021 attgtaaaag tagattaaaa tagagcttgc tcttgcaagc ttttttgatt ttgtgtaaaa
     1081 tagacaaagt gaggtaaagt gatgagtaaa aaagataaaa aaattgaaat ccaactgatt
     1141 gatcacaaag ttatggttaa tgagactaag attgatggct atcaattaca aattggtaaa
     1201 cgtgtggttg gagaaattgc agaacttgat gagaaatttg ctgtcttaaa aaatgatgga
     1261 gtagattgct tcttcaaaac gctcgaacaa gcggttgaaa atatcattga aaactacaat
     1321 ttgaaccatt aaaattagac aaaaacactt gccagcttac aaaaactatg atagaatagt
     1381 ttttgttgtt acggaaagat agcgaagagg ctaaacgcgg cggactgtaa atccgctcct
     1441 tcgggttcgg gggttcgaat ccctctcttt ccatttcgga aactaatcta attagaaaat
     1501 tagttttctg taaaaaatat t
//
LOCUS       DAUCLR010000154         1487 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.157, whole genome shotgun sequence.
ACCESSION   DAUCLR010000154 DAUCLR010000000
VERSION     DAUCLR010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.157"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..727
                     /gene="recX"
                     /locus_tag="VGT87_001795"
     CDS             <1..727
                     /gene="recX"
                     /locus_tag="VGT87_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEQ3814615.1"
                     /translation="DNDESLYVTEDTIVRFMLSKDKVLDNDQLEDMKHFAQLSYGKNL
                     ALYFLSFQQRSNKQVADYLRKHEIEEHIIADIITQLQEEQWIDDTKLADTYIRQNQLN
                     GDKGPQVLKQKLLQKGIASHDIDPILSQTDFSQLAQKVSQKLFDKYQEKLPPKALKDK
                     ITQALLTKGFSYDLAKHSLNHLNFDQDNQEIEDLLDKELDKQYRKLSRKYDGYTLKQK
                     LYQALYRKGYNSDDINCKLRNYL"
     gene            807..1340
                     /locus_tag="VGT87_001796"
     CDS             807..1340
                     /locus_tag="VGT87_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEQ3814616.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENALIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGVSYYCNLASPYMMDTEALKYI
                     DYDLDVKVFADGEKRLLDVDEYEIHKKEMQYSADMDFILKENVKILVDWINHEKGPFS
                     KAYITIWYKRYLELKNR"
BASE COUNT          530 a          279 c          265 g          413 t
ORIGIN      
        1 ggataatgac gaatcccttt atgtaacaga agatactatt gttcggttta tgttgagtaa
       61 agataaagtc cttgacaatg atcagcttga agacatgaaa cattttgccc aactgtccta
      121 cggcaaaaat ttagcccttt attttctttc ctttcaacaa cgcagcaaca agcaagttgc
      181 tgattacctg cgcaagcatg agattgaaga acacattatt gctgacatca tcactcaact
      241 ccaagaagaa caatggatag acgacaccaa attggctgat acctacattc gccaaaatca
      301 gttaaatggt gataaaggtc cccaagtctt aaaacaaaaa ttattacaaa aaggcattgc
      361 aagtcatgac attgatccta tcttatctca aactgacttt agccaactcg ctcaaaaagt
      421 aagccaaaaa ctctttgaca aatatcaaga aaaattgcca ccaaaagcct tgaaagataa
      481 aatcacccaa gcattactga ccaaaggctt ttcatacgat ctagctaaac atagcctcaa
      541 tcaccttaat tttgaccaag ataatcaaga aatagaagat cttcttgaca aagaattaga
      601 caaacaatat cgtaaactca gtcgcaaata tgatggttat accttaaagc aaaagctcta
      661 tcaggctctc taccgaaaag gctacaacag cgacgatatt aattgcaagt taagaaatta
      721 tttatagact gacaaaagaa gtgatttttt cacacattta tgatagacta tagaggataa
      781 gtttaattgt agaaagttgg taaggcatga aattacctaa agaaggcgac tttattacaa
      841 ttcaaagtta taagcatgat ggtagtttgc accgtacttg gcgcgatact atggtactaa
      901 aaacaactga aaatgccctc attggagtta atgatcacac tcttgtcaca gaaagtgacg
      961 gtagacgatg ggtgacccgc gaacctgcaa tcgtttactt tcataaaaaa tactggttta
     1021 acattatagc catgattcga gataatggcg tctcttatta ctgtaactta gctagtccat
     1081 acatgatgga cactgaggcc cttaaataca ttgattacga tttagatgtc aaagtctttg
     1141 cggatggtga aaagagatta cttgatgttg atgaatacga gattcataaa aaagagatgc
     1201 agtattcagc cgatatggac tttatcctaa aagaaaatgt caaaatattg gttgattgga
     1261 ttaatcatga aaaaggacca ttttccaagg cctatatcac tatttggtac aaacgttatc
     1321 ttgaactgaa gaatcgttaa gagttgtcca gcactcccaa ggagtgctca gaacgcagac
     1381 aaactcagtt ttaggctcaa aaagcatgaa cagctctgct gatttgacat ccaatttcct
     1441 atttctgcgt gtggagtgag ccaaaacaga tgtttttcct tgtggta
//
LOCUS       DAUCLR010000155         1435 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.158, whole genome shotgun sequence.
ACCESSION   DAUCLR010000155 DAUCLR010000000
VERSION     DAUCLR010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.158"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..>1435
                     /gene="topA"
                     /locus_tag="VGT87_001797"
     CDS             <1..>1435
                     /gene="topA"
                     /locus_tag="VGT87_001797"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEQ3814617.1"
                     /translation="FQATFYGINGKKTKLDNNNDVKEVLAKLTNEVFLVSKVDKKERR
                     RNAPLPYTTSSLQQDAANKINFRTRKTMMVAQQLYEGIHLGENGTQGLITYMRTDSTR
                     ISPVAQNDAAQFISNRFGANYSKHGNRVKNTSGAQDAHEAIRPSSVNHTPEAIAKYLN
                     KDQLKLYTLIWNRFVASQMTAAVFDTVKVNLEQNGVIFVANGSQMKFDGYMAVYNDSD
                     KNKMLPEMEEGENVKKVSTSPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTLEV
                     IQRRYYVKLSAKRFEPTELGEIVNKLIVEFFPDIVDVAFTAEMEGKLDQVEIGEEEWQ
                     HVIDQFYQPFVKELNKAESEIEKIQIKDEPAGFDCDVCGHPMVIKLGRFGKFYACSNF
                     PECRNTKAITKEIGVTCPVCHKGQVIERKTKKNRIFYGCDQYPDCEFISWDLPIGRAC
                     PKSGDYLIEKKVRGGKQVMCSNETCDYK"
BASE COUNT          469 a          261 c          305 g          400 t
ORIGIN      
        1 gtttcaagcg actttttacg gtattaatgg caaaaaaaca aagcttgata ataacaacga
       61 tgtcaaagag gtattagcaa agctaacaaa tgaagttttt cttgtcagca aggttgacaa
      121 gaaagagcga cgtcgtaatg ctcccttacc ttacacaaca tcatctctac aacaagatgc
      181 tgctaataaa atcaatttca gaacgcgtaa aaccatgatg gtagcccaac aactgtatga
      241 ggggattcat ttaggagaaa acggaactca aggtttaatt acctatatgc gtactgattc
      301 tacacggatt agtcctgtag cgcaaaatga tgctgcccaa tttatcagta atcgttttgg
      361 agctaattat tctaaacatg ggaatcgtgt caaaaatacc agtggtgccc aagatgctca
      421 cgaggctatt cgtccatcta gcgtcaatca tacaccagaa gctattgcca aataccttaa
      481 caaggatcag ttaaaacttt atacgcttat ttggaatcgt tttgtggcta gccaaatgac
      541 agcagctgtt tttgataccg taaaggtgaa cttggagcaa aatggcgtga tttttgttgc
      601 caatggaagc caaatgaaat ttgacggtta tatggctgtt tataatgact cagataaaaa
      661 caaaatgtta cctgaaatgg aagaaggaga aaacgttaaa aaggtatcaa cttctccaga
      721 acagcacttc acgcaacctc cggctcgcta ctccgaagca actctgatta aaactttaga
      781 agaaaatggg gtcggtcgac catcaactta tgccccaacc ttagaagtga ttcaacgcag
      841 gtactatgtt aaattatctg ctaagcgctt tgagccaact gagcttgggg aaattgtcaa
      901 caagcttatt gtcgaatttt tcccagatat tgttgatgtt gcctttactg ccgagatgga
      961 aggtaagcta gatcaagttg aaatcggtga agaagaatgg caacatgtca tcgatcaatt
     1021 ctatcaaccc tttgtcaagg aattgaataa ggctgagtct gaaattgaaa agattcaaat
     1081 caaagatgag ccagctggtt ttgactgtga tgtttgtggc cacccaatgg tcattaagtt
     1141 aggacgtttt gggaaatttt acgcgtgtag caacttccca gagtgccgta atacaaaagc
     1201 catcactaaa gaaattggtg ttacttgtcc ggtttgtcat aaaggacaag ttatcgagcg
     1261 taaaaccaaa aaaaatagga tattttatgg ttgtgatcag tatccagatt gtgagtttat
     1321 ctcgtgggat ttacctattg gacgtgcttg ccctaagtca ggtgactatc ttattgagaa
     1381 aaaagttcgt ggtggcaaac aagtaatgtg tagcaatgag acctgtgatt acaag
//
LOCUS       DAUCLR010000156         1433 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.159, whole genome shotgun sequence.
ACCESSION   DAUCLR010000156 DAUCLR010000000
VERSION     DAUCLR010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.159"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            22..>1433
                     /locus_tag="VGT87_001798"
     CDS             22..>1433
                     /locus_tag="VGT87_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp58-like family protein"
                     /protein_id="HEQ3814618.1"
                     /translation="MSRDPTLILDESNLVIGKDGRVHYTFTAEDDNPKVRLASKCLGT
                     AHFNQLMIERGDQATSYVAPVVVEGTGNPTGLFKDLKEISLELTDIENSKLWSKIKVN
                     NRGMLQEYYDGTIKTEIINSAQGVATRISEDTGKKLALINDTINGIRREYQNADKKLS
                     ASYQAGIDGLKTTMANDKIGLQAEIKASAQGLSQKYDDELRKLSAKITTTSSGTTEAY
                     ENKLNGLRAEFTRSNQGMRTELESKISGLRTAQQTTANQISQEIRNREGAVSRVQQDL
                     ASYQRRLQDAEDNYSSLTHTIRGLQSDVGSPTGKIQSRLTQLAGQIEQRVTRDGVMSI
                     ISGAGDSIKLAIQKAGGINAKMSGNEIISAINLNSYGVTIAGKHIALDGNTTVNGTFT
                     TKIAEAIKIRADQIIAGTIDAARIRVINLNASSIVGLDANFIKAKIGYAITDLLEGKV
                     IKARNGAMLIDLNTAKMDFN"
BASE COUNT          477 a          296 c          336 g          324 t
ORIGIN      
        1 aaaatagatt aaggaggata aatgagcaga gacccaacac ttattttaga cgagtcaaac
       61 ctcgttattg gtaaggatgg acgtgtgcat tacacattta ccgcagagga cgacaaccca
      121 aaagtcagac tagctagcaa gtgtctaggc acagcgcatt ttaatcagct catgattgag
      181 cgaggagacc aagctactag ctatgtagca ccagtagtag ttgagggtac aggtaatccg
      241 actggactat ttaaagacct caaggagatt agtctagagc tgacagacat cgaaaattcc
      301 aagttatggt caaaaatcaa ggtaaacaac cgaggtatgt tgcaggagta ctacgacggc
      361 acgattaaga ctgagattat caactccgca caaggtgtgg ctacacgcat cagtgaggac
      421 actggcaaaa agctagcact tatcaatgac actatcaacg gtataaggcg agagtatcaa
      481 aacgctgata aaaagctatc agctagctat caagctggca tagacggctt aaaaaccaca
      541 atggccaatg ataaaatcgg tttacaagct gagattaaag cctcagcaca agggctatcg
      601 caaaagtatg atgatgagtt gcgcaagtta tctgctaaga tcacaacaac ctcaagcggc
      661 acgaccgagg cctacgaaaa caaactcaat ggcttacgtg ctgagtttac tcgctcaaat
      721 caaggtatgc ggacagaact cgagtctaaa atcagcggac taagaacggc gcaacagacc
      781 actgctaacc aaatctcaca agagatacgt aaccgcgaag gtgctgtcag ccgtgtgcaa
      841 caggacttag ctagctatca gcggcgattg caggacgcag aggataatta cagtagctta
      901 acccatacaa ttagaggttt gcagagtgat gtggggtccc cgactggtaa aatccaatca
      961 cgccttactc aactagcagg acaaattgag cagcgggtta ctagagatgg tgtcatgagt
     1021 attattagtg gcgctggaga cagcattaaa ttagctatcc aaaaggctgg cggcattaat
     1081 gccaaaatgt ctggtaatga gattatctca gcaattaacc tcaactccta cggagtaaca
     1141 atcgcaggta aacatatcgc tctcgatggc aatacgactg tcaacggtac ctttaccaca
     1201 aagatagcag aggctatcaa gattagggct gatcagatta ttgcaggcac gattgacgct
     1261 gctaggatta gagtgattaa ccttaacgca agtagtatcg ttggtttaga cgctaacttt
     1321 atcaaagcta aaattggcta tgctatcact gatttgctcg agggtaaggt cattaaggct
     1381 cgtaatggag cgatgcttat cgacttaaat acagctaaga tggactttaa tag
//
LOCUS       DAUCLR010000157         1338 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.160, whole genome shotgun sequence.
ACCESSION   DAUCLR010000157 DAUCLR010000000
VERSION     DAUCLR010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.160"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            577..1284
                     /locus_tag="VGT87_001799"
     CDS             577..1284
                     /locus_tag="VGT87_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEQ3814619.1"
                     /translation="MSKKKRKHYQVYEGLRCAMTLCFISGYVNAFTYMTQGKRFAGVQ
                     TGNLLSFAIRLSEQQLKEALQFLLPMIVFMLGQSFTYFMHRWATKKGLHWYLLSSVML
                     TGLAFGTALFTPFLPSNVTVAALAFFASIQVDTFKTLRGASYANVMMTGNIKNAAYLL
                     TKGLYEKNHELTHIGRNTLIVILAFAVGVVCSTLLCIAYGEYALMPILMPLLYVNYLL
                     AQEFYHIQTKIKPIKRT"
BASE COUNT          404 a          282 c          262 g          390 t
ORIGIN      
        1 aagaacaacg aacaatgacc tcgaatcacc aaaaggactt gatttgatag cttccctatg
       61 ctatactaag cctattcttt caagaaggac tccttttgtg gaaaagactg aaaaaaagga
      121 cactaatcta tccaaaaact ccagatgagt agctgcaacc ggatgcctct gctgtgatca
      181 aggtgataaa gcagactggc ttgtcactac ctgagcaggt gtcaaaaaaa gaagctttgg
      241 ctttgtcatt caacatactg agcttcagtc tttcagaacg gagacacccc cagttttgta
      301 ctcaaaaagc ataaacagct ctgctgatgc gacatccgat ttctacgtcc gcagtgagcc
      361 aaaccagctg ctgtttgcta tggaaaaagt ggcgccaatc gggatttttg agaccctagg
      421 ctcaaaaata aggcatagaa tcccaaagga agtcgctgcc gtgagcacaa cctaagaaaa
      481 actatcccaa aagacttttt ctgcaatttg aaatactgaa tttcagtatt ttttcttttg
      541 taactaagag caatgaatga ggtaaaaaag gcaacaatgt caaaaaagaa acgaaaacac
      601 tatcaggttt atgagggatt acgctgtgct atgacgcttt gttttatcag tggctatgtc
      661 aatgccttta cctatatgac acaaggcaaa cgttttgcag gtgttcagac aggcaatctt
      721 ttgtcatttg ccattcgctt gtcagaacag caactgaagg aggcactgca atttttactt
      781 cccatgatag tttttatgct tgggcagtcc tttacttatt tcatgcaccg ctgggccact
      841 aaaaagggac tccactggta tctcttatca agcgttatgt taacaggact tgcttttgga
      901 acggccctat tcacgccttt tcttccttcc aatgttacgg tagcagcttt agcctttttt
      961 gcctctattc aggttgatac gtttaaaacc ttacgaggag ctagctatgc taatgtgatg
     1021 atgacaggaa atatcaaaaa tgccgcctat ttactaacca aaggcttgta cgaaaaaaat
     1081 cacgaactaa cacatatcgg acgcaacacc ttaatagtca tcttggcttt tgctgttgga
     1141 gtggtttgtt cgacgttgct ttgcatagct tatggcgaat atgctttgat gcctatttta
     1201 atgcctttac tctatgtcaa ttacctgttg gctcaggaat tttatcacat tcaaaccaaa
     1261 atcaaaccga ttaaaagaac ttaacggaat tgtggagaaa gaaagttcat ttagggtaag
     1321 tttttactgc ccctcctt
//
LOCUS       DAUCLR010000158         1311 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.161, whole genome shotgun sequence.
ACCESSION   DAUCLR010000158 DAUCLR010000000
VERSION     DAUCLR010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.161"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            28..810
                     /locus_tag="VGT87_001800"
     CDS             28..810
                     /locus_tag="VGT87_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEQ3814620.1"
                     /translation="MLCLLLYPLAACNKSEQLNHHERIKKTRKLVVAVSPDYAPFEFK
                     ALVNGKDTIVGADVQLAQAIADELDVDLELSPMSFDNVLSSLQTGKADLAISGISHTK
                     ERAKVYDFSIPYYQAENAIVMRASDAKVTKNISDLNGKKVAAQKGSIEEGLVKIQLPK
                     ANLISLTAMGEAINELKAGQVYAVTLETPVAAGFLAQHKDLALAPFSLKTSDGDAKAV
                     ALPKNSGDLTKAVNKVIAKLDEQERYKSFIAETIALTKNTMK"
     gene            951..1127
                     /locus_tag="VGT87_001801"
     CDS             951..1127
                     /locus_tag="VGT87_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEQ3814621.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVTKAGTLQESRKREFYEKPSVK
                     RKRKSEAARKRKKF"
BASE COUNT          471 a          209 c          240 g          391 t
ORIGIN      
        1 aattttaaaa aaataagatt tggttttttg ctttgcctcc tactttatcc tttagcagcc
       61 tgtaacaagt ctgaacaact taatcaccac gaaagaatta aaaaaacaag aaaattagtc
      121 gttgctgtaa gccctgatta tgctcctttt gaatttaaag ctttagttaa tggaaaagat
      181 actattgttg gagcagatgt tcaattagct caggctattg ctgatgaatt agacgttgat
      241 cttgagcttt caccaatgag ttttgataat gttttgtcta gtttacagac tggtaaagct
      301 gatcttgcta tttcaggaat ctcacatact aaagaaagag ctaaagtata tgatttttca
      361 attccttact atcaggcaga aaacgctata gtgatgagag catctgatgc taaagtgacc
      421 aaaaatatta gtgacctaaa cggtaagaaa gtagccgctc aaaaaggtag tatcgaggaa
      481 ggtttagtta aaatacaatt accaaaggcc aatctgattt ctttgactgc tatgggagaa
      541 gccattaatg aactaaaagc aggacaagtc tatgcagtta ccttagaaac acctgtagct
      601 gctggttttt tagcccaaca taaggatctg gctttagcac cctttagctt aaaaaccagt
      661 gatggagatg ccaaagcagt ggcccttcct aaaaatagtg gtgacttaac caaagccgtt
      721 aataaggtta ttgctaagtt agatgagcaa gaacggtaca agtcatttat tgcagagaca
      781 atagcactaa cgaaaaatac tatgaagtaa taaggttaat aaaaacttta ttagtatccg
      841 ttggaaaagt gtttggaata aacaataaaa gaggtttgac tttttttaaa aaataatgta
      901 atataattgg gaacgtgaaa atgttacaat atcttgagga ggtgaaacac atgtcaaaaa
      961 cagtagtacg taagaatgaa tctcttgacg atgctcttcg tcgcttcaaa cgttctgtta
     1021 ctaaagctgg tactcttcaa gaatcacgta aacgtgaatt ctacgaaaaa ccttctgtaa
     1081 aacgtaaacg taaatcagaa gcagctcgca aacgtaaaaa attctaatgg taaaaaataa
     1141 aaacagttct cacaattaga actgttttta ttttttatcc agaagaagca agggagcttt
     1201 tttgatatca aaaaaagcag ttagacatgc ctaactactt tatcaaaaat cgatttattt
     1261 ttgagccaac aaatcacgga tttctgtcaa taattcttct tgagtaggag c
//
LOCUS       DAUCLR010000159         1259 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.162, whole genome shotgun sequence.
ACCESSION   DAUCLR010000159 DAUCLR010000000
VERSION     DAUCLR010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.162"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..>1259
                     /gene="purB"
                     /locus_tag="VGT87_001802"
     CDS             <1..>1259
                     /gene="purB"
                     /locus_tag="VGT87_001802"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEQ3814622.1"
                     /translation="SRYSRPEMAAIWTEENKYRAWLEVEILADEAWAELGEIPKEDVV
                     KIREKADFDIDRILEIEQDTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAYGY
                     LYKQANDIIRRDLENFTNIVADKAREHKMTIMMGRTHGVHAEPTTFGLKLATWYSEMK
                     RNIERFEHAAAGVEAGKISGAVGNFANIPPFVEQYVCYKLGIRPQEISTQVLPRDLHA
                     EYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSENMT
                     GLARVIRGHMVTAYENVSLWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNLTV
                     FPENMMRNMESTFGLIYSQRVMLKLIEKGMTREEAYDLVQPKTAYSWDNQVDFKPLLE
                     EDIKVTSCLTQEEIDELFNPIYYTKRV"
BASE COUNT          354 a          259 c          307 g          339 t
ORIGIN      
        1 tttcacgtta ttcacgccct gagatggcgg caatttggac agaggaaaat aaataccgtg
       61 cttggttgga ggttgagatt ttggctgacg aggcatgggc tgagttgggt gagattccta
      121 aggaggatgt ggttaagatt cgtgagaagg cggatttcga cattgaccgc attcttgaga
      181 ttgagcagga cacacgtcac gatgtggtgg ctttcactcg tgcggtttct gaaacgcttg
      241 gtgaggagcg caagtgggtg cactacggtt tgacgtcaac tgacgtggtg gacactgcct
      301 acggttacct ctacaagcaa gctaacgaca ttatccgtcg cgatcttgag aatttcacca
      361 atatcgtggc agacaaggcg cgtgagcaca aaatgaccat catgatgggt cgtacccacg
      421 gtgttcacgc cgagccaacg actttcggtc ttaagttggc gacttggtac agcgagatga
      481 aacgtaatat tgagcgtttt gaacatgctg ccgcaggtgt ggaagctggt aagatttcag
      541 gtgccgttgg taactttgct aatatccctc catttgtgga acaatatgtc tgttacaaat
      601 taggcattcg tccacaagag atttcaacac aagttcttcc acgtgacctc cacgcagaat
      661 attttgcagt gcttgcaagt attgcaactt ctatcgaacg tatggcgaca gagattcgag
      721 gtctgcaaaa gtcagaacaa cgtgaagttg aagaattctt tgccaaaggt cagaaaggta
      781 gctctgctat gcctcacaaa cgcaacccaa tcggttcaga gaacatgaca gggctagcgc
      841 gcgtgattcg tggtcacatg gtgacagctt atgagaacgt gtcactttgg catgagcgtg
      901 atatttcaca ctcatcagct gagcgtatca tcacacctga cacaactatc ttgattgact
      961 acatgctcaa ccgctttggc aatatcgtta agaacttgac tgtcttcccg gaaaatatga
     1021 tgcgcaatat ggaatcaact tttggtttga tttatagtca acgtgttatg ctcaaattga
     1081 ttgaaaaagg aatgacacga gaagaagctt atgacttagt tcaacctaag acagcttatt
     1141 cctgggacaa tcaagtggat ttcaaaccac ttttagaaga agacatcaaa gttacctctt
     1201 gtcttacaca agaagaaatt gatgaactat ttaatccgat ttattacaca aaacgtgtt
//
LOCUS       DAUCLR010000160         1211 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.163, whole genome shotgun sequence.
ACCESSION   DAUCLR010000160 DAUCLR010000000
VERSION     DAUCLR010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.163"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            33..1175
                     /locus_tag="VGT87_001803"
     CDS             33..1175
                     /locus_tag="VGT87_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEQ3814623.1"
                     /translation="MWIEETDNGKFKFRENYKDPYTGTWKPVSVTMEKDNSRAYKAAR
                     KILEQKITEKIAQLKASELLFTELLDEWWAFYKKELKRSSVASLRGNIEEIRETFGIG
                     VKVVNIDPKYVQNYLDNLDCSRNKKERNKSMLNLAFDYAVGLDIIQDNPARRAKLPRV
                     KKTLEDWKKAEEKYLEEDEIKPLLKELYRRPSTYRLGLLAEFISLNGCRIGEAVSIEP
                     CNYESKSRILQLHGTFDHTEGYRNGEKTAPKTLASYRETIMTSRELEILQELEFMNEL
                     EKNTNHRYRDMGYLFTTKNGVPIQTNSFNLALKKANERLEDPITKKLTSHIFRHTLIS
                     RLAENNVPLKSIMERVGHADAKTTAQIYTHVTKKMKSSVADIMENY"
BASE COUNT          464 a          199 c          227 g          321 t
ORIGIN      
        1 cctattttaa cagaaaatga ggtaaaaaac aaatgtggat agaagaaact gataacggta
       61 aattcaaatt tcgtgaaaat tataaagatc cttacactgg aacatggaaa cctgtatccg
      121 ttactatgga gaaggataat tcaagagctt ataaagcagc tcgaaaaatc cttgaacaaa
      181 aaataacaga aaaaatagcg caattaaagg cctctgagtt acttttcacg gaacttttag
      241 atgaatggtg ggcgttttat aagaaagaac ttaaaaggtc gtctgtagct tctctgagag
      301 gtaatatcga agagataagg gaaacttttg gaataggtgt taaagtagtg aatattgatc
      361 ctaaatacgt tcagaattat ctcgataacc tagattgctc taggaataaa aaagagcgta
      421 ataagtctat gctaaactta gcatttgatt atgctgttgg tttggatatt atccaagata
      481 atcctgcaag acgtgctaaa ctcccaaggg taaagaaaac tcttgaagac tggaaaaagg
      541 ctgaagaaaa atatcttgaa gaagatgaaa ttaaaccatt attgaaagaa ttgtacagaa
      601 gacctagcac ttaccgactt ggtttgttgg ctgagtttat cagtttgaac ggttgtcgta
      661 tcggtgaagc tgtcagcatt gagccgtgca actacgaatc taagtcaaga atattgcagt
      721 tgcacggaac atttgatcat acagaaggat atcgtaacgg ggaaaagaca gcaccaaaga
      781 cattagcttc ttatcgtgaa accatcatga caagtagaga acttgaaatt ctacaagagt
      841 tagaatttat gaacgaacta gaaaaaaata caaatcatag atatagagat atgggatacc
      901 tttttacaac aaaaaacggt gttccaattc agactaactc attcaactta gctttaaaaa
      961 aagctaatga aagattggaa gacccaatta caaaaaaact tactagccac atcttccgtc
     1021 acactcttat tagccgtcta gcagagaata acgtgccgct aaaatcaatc atggaacgtg
     1081 taggacatgc agacgctaaa actactgccc aaatttatac tcatgtcaca aagaaaatga
     1141 aatcaagtgt agctgatatt atggaaaact attaaatttt ttaccccttt tttacccccc
     1201 tgaacacaaa a
//
LOCUS       DAUCLR010000161         1116 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.164, whole genome shotgun sequence.
ACCESSION   DAUCLR010000161 DAUCLR010000000
VERSION     DAUCLR010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.164"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..290
                     /locus_tag="VGT87_001804"
     CDS             <1..290
                     /locus_tag="VGT87_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019118581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEQ3814624.1"
                     /translation="ISNNELRNNDFSKGQKPYGIYKNIFLTDEEYKDLTNELGSRINE
                     YIDRLSSYMKANNREYQDHKATIINWYLNDQAKNINDNSTRKMNYDIGESL"
     gene            287..1114
                     /locus_tag="VGT87_001805"
     CDS             287..1114
                     /locus_tag="VGT87_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEQ3814625.1"
                     /translation="MKNLKDFVLRENDIERNGHIYCKVCGKRVDGELVDLGFTKFIPR
                     IKCECEIKRDKENEEKERLMKISSLKRDCFSSPLQHQYTFEKFLNEKGQAYKVAYNYA
                     KSFEQMKKDNVGLLFYGDVGSGKTYLACSIANELIERKQVKVKIMNLSQVINQIQKSA
                     FKLDSNEIINNLSNIPLLILDDLGIERDTSYAREQVYNIINSRYLKGRPTIFTTNLSL
                     EIIQNPDIDLEYQRIYSRILEMTIPVKVTGEDFRRKIHQEKLRKYKELLLYGGGIDD"
BASE COUNT          470 a          118 c          207 g          321 t
ORIGIN      
        1 atattagtaa taatgagtta agaaataatg attttagtaa agggcaaaaa ccttatggaa
       61 tatataaaaa catatttttg actgatgaag aatacaagga cttaacaaat gagttaggaa
      121 gtagaattaa tgaatacatt gatagattgt cgtcatatat gaaagcaaat aacagagagt
      181 atcaagatca taaggcaacg ataatcaatt ggtatcttaa tgatcaggca aaaaatatta
      241 atgacaattc aacaagaaag atgaattacg atataggaga gagtttatga agaacttaaa
      301 agattttgta ttgagagaaa atgatattga aagaaatgga catatttatt gtaaggtatg
      361 tggtaaaaga gtagatggag aattagttga ccttggattt acaaagttta ttcccagaat
      421 taaatgtgag tgtgaaataa aaagagataa ggaaaatgaa gaaaaagaaa gactgatgaa
      481 aatatcgtca ttgaaaagag attgtttttc atcgccgctt caacaccaat atacttttga
      541 gaaattccta aatgaaaagg gacaagccta taaggttgcc tataattacg ccaaaagctt
      601 tgaacaaatg aaaaaagata atgttggact tttattttat ggagatgttg gtagtgggaa
      661 gacttatctt gcttgttcaa ttgcaaatga attaatcgaa agaaagcaag ttaaagttaa
      721 gattatgaat ttatctcagg ttataaacca aatacaaaaa tcagcattta agctagattc
      781 aaatgaaatt atcaataacc tctctaatat tccgttgtta atattagacg accttggaat
      841 tgaaagggat acatcttatg caagagaaca agtatataac attataaact caagatactt
      901 aaagggtagg ccgacaattt ttactacaaa cttatcattg gaaattattc aaaatccaga
      961 cattgacctt gagtatcaga gaatatattc aagaatactt gaaatgacaa taccagttaa
     1021 agttacggga gaagatttta gaagaaaaat ccatcaggag aagttaagaa aatacaaaga
     1081 gttactttta tatggaggtg gaatagatga ttaatg
//
LOCUS       DAUCLR010000162         1105 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.165, whole genome shotgun sequence.
ACCESSION   DAUCLR010000162 DAUCLR010000000
VERSION     DAUCLR010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.165"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            44..508
                     /locus_tag="VGT87_001806"
     CDS             44..508
                     /locus_tag="VGT87_001806"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814626.1"
                     /translation="MINKFQRPSKKYYFLIALASLVLLGTSKTVFGESMTLYTGDYWY
                     EEDGGMLNGNHWGMENWWEPSSLWAPYPNPHYSENDPHQPPYGGSLLGLSSDNERERG
                     PRPTPSPQSPLTPESNPNNSRNQGPVNSPWPQGPLFGGLTSGTTSSWGSVIK"
     gene            813..>1105
                     /locus_tag="VGT87_001807"
     CDS             813..>1105
                     /locus_tag="VGT87_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_150395460.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEQ3814627.1"
                     /translation="MTRQQTKKNYSLRKLKTGTASVAVALTVLGAGFANQTEVRADQA
                     ASEKVEVKESEKETKYKTLALRGENADLRNVNAKYLEKINAEEEKNKKLEATN"
BASE COUNT          406 a          194 c          220 g          285 t
ORIGIN      
        1 ctaaaacgca aagaagaaaa ctaaaatata aaagggatca atgatgatta acaaatttca
       61 acgtccgtct aaaaaatatt attttcttat cgctttggca agtttagtgc tgttgggaac
      121 ttctaagacc gtctttggag aatcaatgac actgtataca ggagattatt ggtacgagga
      181 agatggtggt atgttaaacg gaaaccactg gggaatggaa aattggtggg agccatcaag
      241 tttgtgggct ccttatccaa atcctcacta tagtgaaaac gatccacacc agccacctta
      301 tggcggatcc ttattgggat tatcctcaga taatgagaga gaacgtgggc ctagacctac
      361 tccatcgcca cagtctcctt taactccgga gtcaaatcct aacaactcta gaaatcaggg
      421 tcctgttaat agcccatggc ctcaaggtcc gctttttgga ggattaacaa gtggaactac
      481 ctccagttgg ggaagtgtta ttaaatagtt tgttataagt tatccataat ttctaaatca
      541 aacaaccgcc ccattcttta cagtagcagg tatgcttgct ctaaaacgta aagaagaaaa
      601 ctaagctatt agactgatgc taaagctaag agagaatcaa atgattctct ctttttgagt
      661 agctaagtaa ctaacaatct cagttagacc aaaaaatgag aatggctcaa aaagctggcc
      721 tttactcctt ttgattaacc atatataata aaaacgttag gaaaataata gcactattaa
      781 gttgcttttt caataaaatc aaggagtaga taatgactag acaacaaacc aagaaaaatt
      841 attcactacg gaaactaaaa accggtacgg cttcagtagc cgttgctttg accgttttgg
      901 gcgcaggttt tgcaaaccaa acggaagtaa gagctgatca agcagcttct gaaaaagtgg
      961 aagtaaaaga aagtgaaaaa gagaccaagt ataagacgtt ggccttaaga ggtgaaaatg
     1021 ctgaccttag aaatgtaaat gcaaaatatt tagagaaaat taacgcagaa gaagaaaaaa
     1081 ataaaaagct tgaagcaact aataa
//
LOCUS       DAUCLR010000163         1042 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.166, whole genome shotgun sequence.
ACCESSION   DAUCLR010000163 DAUCLR010000000
VERSION     DAUCLR010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.166"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..312
                     /locus_tag="VGT87_001808"
     CDS             <1..312
                     /locus_tag="VGT87_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021340616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814628.1"
                     /translation="DKKPEAKPEQDGSGQTPDKEPETKPEKDSSGQTPGKTPQKGQPS
                     RTLEKRSSKRALATKASTRDQLPTTNDKDTNRLHLLKLVMTTFFLGLVAHIFKTKRTE
                     D"
     gene            409..>1042
                     /locus_tag="VGT87_001809"
     CDS             409..>1042
                     /locus_tag="VGT87_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285739.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEQ3814629.1"
                     /translation="MRRAENNKHSRYSIRKLSVGVTSIAIASLFLGKVAYAVDSMQQN
                     SSTQRNTANTSKKWYHIDDRGFIPVDISLETAKEDFKKEVKESRLSEEQQKTYNQKIE
                     AAKNKDDLLLTYHREYSEDIKKHPASTEPTTQPVEAPVQETQAPASDSMVTGDSTSVT
                     ADSPKETPSSESPVAPAPSIPEVPEAPASSPESEKSSVAASSEETSSPETP"
BASE COUNT          382 a          225 c          198 g          237 t
ORIGIN      
        1 gacaaaaaac cagaagctaa accagaacaa gacggttcag gtcaaacacc agataaagaa
       61 ccagaaacta aaccagaaaa agatagttca ggtcaaacac caggtaaaac tcctcaaaaa
      121 ggtcaacctt ctcgtactct agagaaacga tcttctaagc gtgctttagc tacaaaagca
      181 tcaacaagag atcagttacc aacgactaat gacaaggata caaatcgttt acatctcctt
      241 aagttagtta tgaccacttt cttcttggga ttagtagctc atatctttaa aacaaaacgc
      301 actgaagatt agtacaactg atcagatggc tactattgat gatgagttag atcagtcttc
      361 ttttcaaaag aaggcatagc agtataaaaa tagatatgtg aggacaatat gcgtagagca
      421 gaaaataaca aacacagccg ctattccatt cgcaaactga gcgttggggt aacgagtata
      481 gcaattgcga gtctcttttt aggaaaggtt gcctatgccg tagatagcat gcagcaaaac
      541 tcttcgactc aaagaaacac agccaataca tccaaaaaat ggtatcatat tgatgatagg
      601 ggctttattc ctgtagatat aagcttagaa actgccaaag aggattttaa aaaagaagta
      661 aaagaatcac gtttgtctga agaacaacag aaaacgtata atcaaaaaat tgaagctgca
      721 aaaaacaaag acgacctatt gttaacatat catagagagt actctgaaga tattaagaaa
      781 cacccagcgt ctactgagcc tactactcag ccagttgagg cacccgtgca ggagacacag
      841 gccccagctt cagattcgat ggtgacaggt gattcaacat cagttacggc tgattctcct
      901 aaggaaaccc catcttcgga aagtccagta gccccagctc catcaattcc agaggtccca
      961 gaagccccag cttcatcacc tgagagtgag aaatcttcag tagcagcttc ttctgaggaa
     1021 acctcatctc cagaaactcc ag
//
LOCUS       DAUCLR010000164          759 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.167, whole genome shotgun sequence.
ACCESSION   DAUCLR010000164 DAUCLR010000000
VERSION     DAUCLR010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.167"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            504..620
                     /locus_tag="VGT87_001810"
                     /pseudo
     CDS             504..620
                     /locus_tag="VGT87_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611995.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          280 a          134 c          101 g          244 t
ORIGIN      
        1 cttattttaa aaataaagtt aacacaacaa ggacaaggct agctcaattg tactgttgta
       61 acacaacaac tatctagttc ttatcacttt tattatagta aaaaatgatt aatatgtaaa
      121 ccctttcttt ttaatattat caaaaatgac aaaaaaaata ataattgatt aatatagaaa
      181 caattattat ctaatatact gaattttata atagattcta tcaataacta tgtgtaaaca
      241 atcaagtaga aatgtcttta ttaagcgaat caatcaaggt tgtggagtaa atgctggact
      301 agatttcatg tagaaattag taaaagttag gagatttttt ggtcgtttca aacgataaca
      361 tcatcttctt ataacaagaa cttcactcag agataaactg ttctaatact ttagtagcct
      421 cttttgagct tgtagcacga aaattcgctc gaactgattt aaaataggca ttttatttgc
      481 tcgcttgatc cctactgcgc gaccaagaaa ccttttattc actagaacaa ttaaccctta
      541 aaaccaagga ggaagtccac tggtggaact accatcacct tcgcagtaat cttaattatc
      601 aaacacccat aactaaatga gtcatcgttt gaacaaaaaa cgccttataa attctgtaca
      661 gaaaactgtt gccttttcaa ttgaactttg aaatgttttc aatacactcc gttcttgggc
      721 ccttacgatc caaaaaacca aggtgatttc aaaatcacc
//
LOCUS       DAUCLR010000165          749 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.168, whole genome shotgun sequence.
ACCESSION   DAUCLR010000165 DAUCLR010000000
VERSION     DAUCLR010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.168"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            66..>749
                     /locus_tag="VGT87_001811"
     CDS             66..>749
                     /locus_tag="VGT87_001811"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEQ3814630.1"
                     /translation="MSRKPIIAGNWKMNKNPQEAKAFVEAVASKLPSTDLVDVAVAAP
                     AVDLVTTIEAAKDSVLKVAAQNCYFENTGAFTGETSPKVLAEMGADYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGLTPIVCCGESLETYEAGKAVEFVGAQVSAALAGLSAEQV
                     ASLVLAYEPIWAIGTGKSATQDDAQNMCKAVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVKDYMACPD"
BASE COUNT          235 a          135 c          168 g          211 t
ORIGIN      
        1 ttttaatcgt cggtcatttg tgctaaaata gaagatataa ataaaaaaaa gaagaggtat
       61 gtgatatgtc acgtaaacca attattgctg gtaactggaa aatgaacaaa aaccctcaag
      121 aagcaaaagc attcgttgag gctgtagcaa gcaaattacc ttcaactgac cttgttgatg
      181 ttgccgtagc agccccagct gttgatttgg taaccactat tgaagctgct aaagattcag
      241 ttcttaaagt tgctgcacaa aactgctact ttgaaaacac aggggcattc actggtgaaa
      301 catcaccaaa agtgcttgct gaaatgggag ctgactatgt tgtgattggt cactcagaac
      361 gccgtgatta cttccatgaa acagatgaag acattaataa aaaagccaaa gcaatctttg
      421 ctaacggttt aactccaatt gtttgttgtg gtgagtctct tgaaacttat gaagctggta
      481 aagccgttga gtttgtaggt gcacaagttt cagcagcact tgcaggtctt tcagcagagc
      541 aagtagcttc acttgtttta gcttatgagc caatctgggc tatcggtact ggtaaatcag
      601 ctactcaaga tgatgctcaa aacatgtgta aagctgttcg tgacgtggta gcagcagact
      661 ttgggcaaga agttgctgat aaagttcgcg ttcaatacgg tggttctgta aaacctgaaa
      721 acgttaaaga ttacatggct tgtccagat
//
LOCUS       DAUCLR010000166          719 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.169, whole genome shotgun sequence.
ACCESSION   DAUCLR010000166 DAUCLR010000000
VERSION     DAUCLR010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.169"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(260..562)
                     /locus_tag="VGT87_001812"
     CDS             complement(260..562)
                     /locus_tag="VGT87_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814631.1"
                     /translation="MTYELCLEYGTYPLSRVDAYWGEDQNPPTFIQEDRLLCHKLETM
                     NHLFHDLFVTIESQFHYVGFNMPKKRAQIRILYQEVATILKSKYKDYPIKIETFLL"
BASE COUNT          234 a          106 c          119 g          260 t
ORIGIN      
        1 tatttttctg accttgaaaa cgatgagtat aggtcttaaa tgtctctgta atgccacagc
       61 ctgaatttta tttgactcag attcataaga ttgtaagtaa tcaaggttaa ttattgatga
      121 gatggtgaaa ccatttttag caagtgctat taaagcattt agtgattttt cgaaacgttt
      181 taaaatttaa gaagagtttt acctaaaaaa ctagctgtgt catctatcta agcacagtta
      241 gttttttagt attagatttt taaagaagaa acgtctcaat cttaattgga taatccttat
      301 atttggattt taatatagta gctacttctt gatatagtat ccgaatctgc gctctttttt
      361 taggcatgtt aaaaccaaca taatggaatt gtgattcaat agttacaaat aaatcatgga
      421 acaggtgatt catagtttct aatttatggc aaagcagtcg atcttcttga atgaatgttg
      481 ggggattttg atcttctccc cagtaagcat caacacgtga caacggataa gtcccatatt
      541 ctaaacaaag ttcataagtc attttggcct ccttgtttta tcttagcatt ttctaaataa
      601 agcttctaat gatttgctta aaggagtaat gggttgccat ctcatctcat caaaaataaa
      661 ttgagatcta ttttgtgaaa aaaaaagaca aacaaatggc ttgcctagac ttgtttttc
//
LOCUS       DAUCLR010000167          706 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.170, whole genome shotgun sequence.
ACCESSION   DAUCLR010000167 DAUCLR010000000
VERSION     DAUCLR010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.170"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            78..>706
                     /locus_tag="VGT87_001813"
     CDS             78..>706
                     /locus_tag="VGT87_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407885.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEQ3814632.1"
                     /translation="MARKDTNKQYSLRKLKTGTASVAVAVAVLGAGFTNQTTVKADSS
                     SRDITEAGVSKFWKSKFDAEQNRANELEKKLSGYEKDYKTLEQEYENLEETLQKFGND
                     YERLENKNNEYANQLGKNQEEREKLELEYLRKSDKEYKEHQQYRQEQEERQKNLEELE
                     RQNKREIDKRYQEQLQKQQQLETEKQISEASRKSLSRDLEASRAAKKGL"
BASE COUNT          320 a          104 c          140 g          142 t
ORIGIN      
        1 ttagaataga attattagag agaagtctta gaaaaatgag gctaattccc taaagatgaa
       61 aaaataagga gcaaataatg gctagaaaag atacgaataa acagtattcg cttagaaaat
      121 taaaaacagg tacagcatca gtagcggtcg ctgtggctgt tttaggagca ggctttacaa
      181 accaaacaac agttaaggcg gactcttcta gtagagatat cactgaggca ggtgtatcta
      241 agttttggaa gagtaagttt gatgcagagc agaatagagc aaacgagctt gaaaaaaaac
      301 ttagtggata cgaaaaagat tataaaacat tagaacaaga gtatgaaaat ctagaagaaa
      361 ctttacaaaa attcggaaat gattatgaaa gattagaaaa caaaaataat gagtacgcaa
      421 atcaacttgg taaaaatcaa gaagaacgcg aaaaattaga gcttgaatac ctcagaaaat
      481 cagataaaga gtataaagag catcaacaat atcgacaaga acaagaagaa cgtcaaaaaa
      541 atctagagga acttgaacgt caaaataaac gagaaataga caaacgctat caagaacaac
      601 tccaaaaaca acaacaatta gaaacagaaa agcaaatctc agaagctagt cgtaagagcc
      661 taagccgtga cctcgaagct tctcgtgcag ctaaaaaagg ccttga
//
LOCUS       DAUCLR010000168          697 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.171, whole genome shotgun sequence.
ACCESSION   DAUCLR010000168 DAUCLR010000000
VERSION     DAUCLR010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.171"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(293..697)
                     /locus_tag="VGT87_001814"
                     /pseudo
     CDS             complement(293..697)
                     /locus_tag="VGT87_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558053.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          211 a           95 c          134 g          257 t
ORIGIN      
        1 ctatttagta actttggtta aagtaatcga actaacttgg ttgattgtat tttaatgata
       61 aaaagcttaa atttgttata caacaaattt aagcttttta ttgtttcttt ttaattgcag
      121 attgaggttt aaaagacact ttacaatgtt ctcaagtcat gtgttgttgt aggttgtatg
      181 cacatattac aaatcccatt atagaagaaa ttgtttaatt aatggatagt aatggatggt
      241 atggagatct taatgtggtt ttaatggtaa ttttagcagt tatagtaaga caaaaggtga
      301 cttggaaaag cttatgcaga cgattcttaa ctcccacaat atcttctgtc agattttatt
      361 agacttcttc ttggacatga gttggtttac gatttagtac aaattgagat tgagctagtg
      421 tttcagtttc aattttgtct gttttacgaa cgtgcaggct attcaattgc ttcttagctt
      481 ctaaaggatt aaaccgagta taagcgtagc cattatctgc gaaaaaacct gaagacgacg
      541 agaatagaca ttaatcgctt taaggataat ctcagggtta tggacggttt tcaagtttcc
      601 aagaagatga ttgaggccga tggtatcgtt ttgtcttgaa tgattcctta ttttagtggt
      661 ttcattttca atacacgacg tcaagcgtcc tacatac
//
LOCUS       DAUCLR010000169          666 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.172, whole genome shotgun sequence.
ACCESSION   DAUCLR010000169 DAUCLR010000000
VERSION     DAUCLR010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.172"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..>666
                     /locus_tag="VGT87_001815"
     CDS             <1..>666
                     /locus_tag="VGT87_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEQ3814633.1"
                     /translation="AVDKATTAEGINQELGKGITAINKAYRPGEGVKARKEAAKANLE
                     KVAKETKALISGDRYLSETEKAVQKQAVEQALAKALGQVEAAKTVEAVKLAENLGTVA
                     IRSAYVAGLAKDTDQATAALNEAKQAAIEALKQAAAETLAKITTDAKLTEAQKAEQSE
                     NVSLALKTAIATVRSAQSIASVKEAKAKGIAAIRAAYVPNKAVAKSSSANHLPKSGDA
                     NSIV"
BASE COUNT          228 a          134 c          146 g          158 t
ORIGIN      
        1 gcggttgata aagcgacaac agctgaaggc attaaccaag aacttggtaa aggcatcaca
       61 gctatcaaca aagcttaccg tccaggtgaa ggtgttaaag cacgtaaaga agctgctaaa
      121 gctaatcttg aaaaagtagc taaagaaact aaagctctta tttcaggaga ccgttacttg
      181 agcgaaactg aaaaagcagt ccaaaaacaa gctgttgagc aagctcttgc gaaagcactt
      241 ggtcaagttg aggctgctaa gacagttgaa gctgttaagt tggcagaaaa ccttggtact
      301 gtagctatcc gttcagcata tgttgctggt ttagctaaag atactgatca agcaacagct
      361 gctcttaacg aagcgaaaca agctgctatt gaagctctta aacaagctgc ggcagaaaca
      421 cttgctaaga ttacaactga tgctaaattg actgaagctc aaaaagctga acaatcagaa
      481 aatgtatcat tagcgcttaa gacggctatt gcgactgttc gttcagcaca atctattgcg
      541 tctgtgaaag aagcaaaagc taaaggtatt gctgctatcc gtgcagccta tgtgcctaat
      601 aaggcagtcg caaaatcatc gtcagcgaac catcttccaa aatcaggtga tgcaaactca
      661 attgtt
//
LOCUS       DAUCLR010000170          637 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.173, whole genome shotgun sequence.
ACCESSION   DAUCLR010000170 DAUCLR010000000
VERSION     DAUCLR010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.173"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..598
                     /locus_tag="VGT87_001816"
     CDS             <1..598
                     /locus_tag="VGT87_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEQ3814634.1"
                     /translation="YHKKDQWLFKEIDLEVAPGQILGIFGQSGCGKTSLSRVLAGFLQ
                     PKSGEVLVDGSHLPNKAFRPVQLIQQHPEQTMNPLWPMKKSLEEAYYPSQDLRDAFGI
                     QEKWLKRRPSELSGGELQRFSIVRSLHPETKYLIADEMTTMLDSITQASVWKSLLEIV
                     KDRNLGLIIISHDFDMLEKLCDACYMIEENRTQLFKHT"
BASE COUNT          202 a          105 c          136 g          194 t
ORIGIN      
        1 ttatcataaa aaagatcaat ggctttttaa ggagattgat ttagaggtag cacctggtca
       61 aatcttaggg atatttggac aaagtgggtg tggaaaaacg agtttatcca gagtgcttgc
      121 cggttttttg cagcctaaat ctggtgaagt attagttgat ggcagtcatt tgcctaacaa
      181 agcatttaga cctgtgcaac ttatccaaca acatcctgaa caaaccatga atcctttatg
      241 gcctatgaaa aaaagtttgg aagaagccta ctatccaagt caagatttgc gagatgcttt
      301 tggcattcaa gaaaaatggc taaagcgtcg ccctagtgaa ctctcgggag gggaattgca
      361 acgcttttcg attgtgcgtt cattacatcc agagacgaaa taccttattg cagatgaaat
      421 gactactatg ttggatagca ttacacaagc tagtgtatgg aaaagcctgt tggagattgt
      481 aaaggataga aatttaggtc tcatcattat tagtcatgat tttgatatgt tagaaaaact
      541 ttgtgacgcg tgctatatga ttgaagagaa tcgtactcaa ctgttcaagc atacctaaca
      601 tctatggtta ttacctattg agtctttttc taacaaa
//
LOCUS       DAUCLR010000171          607 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.174, whole genome shotgun sequence.
ACCESSION   DAUCLR010000171 DAUCLR010000000
VERSION     DAUCLR010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.174"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..372)
                     /locus_tag="VGT87_001817"
     CDS             complement(<1..372)
                     /locus_tag="VGT87_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEQ3814635.1"
                     /translation="MTRLLKRYPITIFLLGLTGLIFIAMQVVYGHLATGAQAIYQVGG
                     MFGLLVKAMPDQLWRLVTPIFIHIGFGHFFVNGLTLYFVGQIVEDLWGSRRFLLLYVL
                     SGVMGNAFTFWLTPETVAAGAS"
BASE COUNT          221 a          125 c           93 g          168 t
ORIGIN      
        1 cgatgctcct gcagctactg tttcaggagt caaccaaaaa gtaaaggcat tgcccataac
       61 acctgataaa acataaagta ataaaaaacg gcgtgaaccc cagaggtctt cgacgatttg
      121 accaacaaaa tatagtgtta atccattgac aaaaaaatga ccaaagccga tatgaataaa
      181 tatcggagtc accaatcgcc acaactgatc tggcattgct tttactagta atccaaacat
      241 acctccaact tgataaatag cttgagcacc agttgctaaa tgtccatata ccacttgcat
      301 tgctataaaa attaagcctg ttaaccctaa aaggaaaata gtaataggat atcgttttaa
      361 taaccgtgtc atctaagata aacctcctaa acagcaatat cataaatcgt ttgatttggc
      421 aatcgtattg gcccgctgat aagcttgatt gctaacaaca tccactaata aagcttgatc
      481 attttgacgt tttaagtcta catccatagt tcgtaaagac tagaagagtt ctttgagaat
      541 ctcttttttc atcatcttct tatcatacca taaattagga aaaaggttca agactgacaa
      601 gaaacta
//
LOCUS       DAUCLR010000172          589 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.175, whole genome shotgun sequence.
ACCESSION   DAUCLR010000172 DAUCLR010000000
VERSION     DAUCLR010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.175"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            140..343
                     /locus_tag="VGT87_001818"
     CDS             140..343
                     /locus_tag="VGT87_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814636.1"
                     /translation="MSKWELQKQLLENATDFESTVEALLDLNESGEISDLELLLNLKD
                     KFRSYSKEKIRILNEILKLGGAE"
     gene            340..589
                     /locus_tag="VGT87_001819"
                     /pseudo
     CDS             340..589
                     /locus_tag="VGT87_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988700.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          221 a           85 c          124 g          159 t
ORIGIN      
        1 ttagtaacaa ctgttgcttt agcaattgtt gccaggatac aatatgaagt catcaaaaaa
       61 cacaactcgc cagagaataa gcgaaaaatt tttagggaag tagctttaga aaacagcaag
      121 aaatggagtg cagaacgtta tgtctaaatg ggaattacaa aaacaactac tggaaaatgc
      181 gacagatttt gagagcacag ttgaggcttt acttgactta aacgagtctg gcgaaatatc
      241 tgatctggaa ctcttactga acttaaagga taaattcaga agctattcca aagaaaaaat
      301 acgtatcctt aatgaaattc tcaagctagg aggtgcagaa tgatgacaac ttttgaaaag
      361 attattaaga aaattaatcg gattgagggg gagttagtta aacctgaagc tgaaagacga
      421 gatagcttac tatcctatct gctcagttgg gttctattaa acaagattta aaattgatgc
      481 tttggattga gctaccgacg ttaaatgacg tagagagata tgaagctctt tatcaaggaa
      541 atgattttaa attcagtgaa actttcttag agcgtcaagc gacgagaaa
//
LOCUS       DAUCLR010000173          567 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.176, whole genome shotgun sequence.
ACCESSION   DAUCLR010000173 DAUCLR010000000
VERSION     DAUCLR010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.176"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            15..>567
                     /locus_tag="VGT87_001820"
     CDS             15..>567
                     /locus_tag="VGT87_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814637.1"
                     /translation="MINKSNSWEEFLDNMKSIDYEIKFGKHIAFRHKDKQRFTRAKTI
                     GEDYTEEKIKERIDLAIKNKANPIKKRVGNVIDISTNEKAQSSKGYEVWARKHNIKTM
                     ADSIIKLREQGIKSITKLDDLIKKSADDRQDLLDKIKKIESEMKSLSQDMENINTINK
                     YREIYKYHKKNPEDNQFAEEYYSE"
BASE COUNT          255 a           65 c           93 g          154 t
ORIGIN      
        1 ttgatataga tagaatgatc aataagtcta actcgtggga agaattttta gataatatga
       61 aatctattga ttatgaaatt aagtttggta aacacattgc ttttcgtcat aaagataagc
      121 aaagatttac aagagctaag actatcggag aagattatac tgaggaaaaa atcaaagaac
      181 gaatagattt agcaattaaa aataaagcta atcctattaa aaagcgtgta ggaaatgtta
      241 ttgatatatc cactaatgaa aaagctcaat cctcaaaagg ttatgaagtt tgggcaagaa
      301 aacataatat caaaacaatg gctgactcga taattaagct tagagaacaa ggaattaaat
      361 ctattactaa actcgatgat ctaatcaaaa aatctgctga tgatagacaa gacttgttag
      421 ataaaataaa gaaaatcgaa agtgaaatga aaagtttatc ccaagatatg gaaaatataa
      481 atactataaa taagtatcgt gaaatctata aataccacaa gaaaaatcct gaagataatc
      541 agtttgcaga agaatattat agtgaac
//
LOCUS       DAUCLR010000174          545 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.177, whole genome shotgun sequence.
ACCESSION   DAUCLR010000174 DAUCLR010000000
VERSION     DAUCLR010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.177"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(169..525)
                     /locus_tag="VGT87_001821"
     CDS             complement(169..525)
                     /locus_tag="VGT87_001821"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814638.1"
                     /translation="MSRSEGAERTEQQNLFRGPQEDFEEGNDEAGSYTYTWEKDRDFG
                     KQQGLEAGKNGYGPNISRDDISVPVGMQNPDEYKDGYQEGYSEGWHQEHPLLGVVFDV
                     FVSIWGVVSGLFSEAN"
BASE COUNT          157 a          140 c           78 g          170 t
ORIGIN      
        1 cctgactact aaagagacta tcccaattat agcagattag tgtgggaaat gcttgtgata
       61 aaaaattaac tgtagtactt ttagctatat gtaccaaaaa tatcgctgta gcctaaacat
      121 tacagtactt tcaacagata atactactct ctaacagcgt aactataact aatttgcttc
      181 actgaaaaga ccactcacta cgccccagat cgaaacaaaa acgtcgaata caactcctag
      241 aagtggatgc tcctgatgcc acccctcact ataaccttct tgatacccat ctttgtattc
      301 atctggattt tgcataccta caggaactga tatgtcatcc cgacttatat tcggtccata
      361 accattttta ccagcctcaa gtccttgttg ttttccaaaa tctctatctt tctcccatgt
      421 ataagtataa cttccggctt catcgttacc ttcttcaaaa tcttcttgag gacctctaaa
      481 aagattttgc tgctctgtac gctcagcgcc ttcgcttctt gacacctccg cctttacggg
      541 acgtg
//
LOCUS       DAUCLR010000175          526 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.178, whole genome shotgun sequence.
ACCESSION   DAUCLR010000175 DAUCLR010000000
VERSION     DAUCLR010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.178"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(93..>526)
                     /locus_tag="VGT87_001822"
     CDS             complement(93..>526)
                     /locus_tag="VGT87_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEQ3814639.1"
                     /translation="PAAPALSEAPAQPAESEKPSVAASSEETPSPSTPAESETQTPPA
                     VTKDSDKPSSAAEKPAASSLVSEQTVQQPTSKRSSDKKEEQEQSYSPNRSLSRQVRAH
                     ESGKYLPSTGEKAQPLFIATMTLMSLFGSLLVTKRQKETKK"
BASE COUNT          135 a           92 c          127 g          172 t
ORIGIN      
        1 aacttctttt tgatgaggca gcacatctaa aaaaatagag agaaccaaat tggttctctc
       61 tattaaataa gctcttatca gtttaagtaa tactattttt tagtttcttt ttggcgtttt
      121 gtgactaaaa gacttccaaa tagagacatc aaagtcatag tagctataaa gagtggctgc
      181 gctttttcac ctgttgaagg caagtactta cctgactcat gggccctaac ctgtcttgac
      241 aatgagcgat ttggagagta agactgttct tgctcttctt ttttatcaga agatctcttt
      301 gaagttggtt gttgaacggt ttgttctgaa acaagtgaag aggctgctgg tttttcagct
      361 gctgaagatg gcttatcaga gtctttagta acagctggtg gcgtctgagt ctctgattca
      421 gctggagttg atggagatgg ggtttcctca gaagaagctg ctactgaagg tttctcactc
      481 tcagctggtt gagctggagc ctcagataaa gctggggccg ctggag
//
LOCUS       DAUCLR010000176          485 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.179, whole genome shotgun sequence.
ACCESSION   DAUCLR010000176 DAUCLR010000000
VERSION     DAUCLR010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.179"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..>485)
                     /locus_tag="VGT87_001823"
     CDS             complement(<1..>485)
                     /locus_tag="VGT87_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEQ3814640.1"
                     /translation="IADHIESFTDYDFAHLLFSNGYVEQSISLEKQLNIIQVADLVLP
                     DKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTL
                     SMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVD
                     P"
BASE COUNT          147 a           97 c           94 g          147 t
ORIGIN      
        1 ctgggtctac accaaaaaag gctaaggtct ttttaatctc gttaaggtca gtggaacgaa
       61 atgcaaattt taagccgagg ttatttttca gtttttcatc taagaggtcg tctgtatttt
      121 gggtcacgaa atatacccca gcgttcatag cacgaccagc ccgaaccagc ttcatagata
      181 gtgtttttcc ttgtgctacc tgtaaaaagc tccatgcttc gtctaaatct acaatcttga
      241 aaatgcttcg gtctgtatgg ataaagtcta aagcaaaggt actaatgaca atcagcatag
      301 caacggataa aagctccata gtggtatatt cctcaaagga agtttccttg tcgggaagta
      361 ccaagtccgc aacctgtata atgttcagtt gtttttctaa gctgatagac tgctccacat
      421 aaccattact gaataataaa tgtgcaaagt catagtctgt aaaactttcg atatggtcgg
      481 ctata
//
LOCUS       DAUCLR010000177          484 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.180, whole genome shotgun sequence.
ACCESSION   DAUCLR010000177 DAUCLR010000000
VERSION     DAUCLR010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.180"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
BASE COUNT          183 a           68 c           65 g          168 t
ORIGIN      
        1 ggaaaaacta tgaaaattat tatggaaagg aagtggagag ataaaaagct ataagcggat
       61 aaacttatag cttttttaaa attatttaca aatttttatt ctaaacttat tatcttactt
      121 aaagagatcc tactagatga ctagcaaaca ttgctatagg aagtaataag ataaaaagga
      181 catttaatgc cataaaaaac ctatcttttt cccttacccc aagtattaaa cctactagtc
      241 caataattgg acagacaagc atgtgagaat aagcaataaa tacgcatctc ataatttatt
      301 atatcatttt ttataaaaga aacctgctaa caaaagtcaa gtataacctg atataaattt
      361 caggttattt gattatttga gttattaaat tagcgtatag caaacaagcc gagtgtaatc
      421 aatcggcttg ctttttagtg ttattttaaa tcttatgcta cttttaattc ttttttctct
      481 gctt
//
LOCUS       DAUCLR010000178          457 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.181, whole genome shotgun sequence.
ACCESSION   DAUCLR010000178 DAUCLR010000000
VERSION     DAUCLR010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.181"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..>457
                     /locus_tag="VGT87_001824"
     CDS             <1..>457
                     /locus_tag="VGT87_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEQ3814641.1"
                     /translation="ITKSEKTDEKVLVSSFKCHPSTAHIQFLKTREDNDTKGTVLARH
                     LIQSFLPGEVDPIKAHEIGMELCKKILKEDYEFVLATHIDRGHIHNHIIFNNVNYKTG
                     KCYQSNKKSYHKIRYQSDELCKENKLSVIDKYYEAYKRKYKTAGKSWYEY"
BASE COUNT          186 a           66 c           68 g          137 t
ORIGIN      
        1 cattactaag agcgaaaaaa ctgatgaaaa agtcttagta tcttcattca aatgtcatcc
       61 atctactgcc catattcaat ttttgaaaac tcgagaagat aatgatacta aaggaacagt
      121 cttagctaga catttaattc aatcttttct accaggagag gttgatccta taaaagctca
      181 cgaaattgga atggaattat gtaagaaaat tttaaaagaa gattatgaat ttgttcttgc
      241 aactcatata gatagaggac atatccataa ccatataatt tttaataatg ttaattacaa
      301 gactggtaaa tgctaccaat ctaacaaaaa atcttaccac aaaatcaggt atcaaagtga
      361 tgaattatgt aaagaaaata agctttcagt cattgataaa tactatgaag cttacaagag
      421 aaaatataaa actgctggta agtcttggta cgaatat
//
LOCUS       DAUCLR010000179          451 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.182, whole genome shotgun sequence.
ACCESSION   DAUCLR010000179 DAUCLR010000000
VERSION     DAUCLR010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.182"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            48..>451
                     /locus_tag="VGT87_001825"
     CDS             48..>451
                     /locus_tag="VGT87_001825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HEQ3814642.1"
                     /translation="MNKTKQNKTKHSLLCRYGLTSAAALLLTFGGVSAVKADEQSNIE
                     ALQSQLQALRENLPKVGDNIPKHDVFRQHVFYKHLEEFEARLDFYLKTLDGSLSQLQG
                     PRGEKGDQGPRGEQGLRGEIGPEGPQGKTGPAG"
BASE COUNT          148 a           88 c          111 g          104 t
ORIGIN      
        1 gtgagcctgc atttaatctt attaaagaga aagaggaaac aaaaactgtg aacaaaacaa
       61 aacaaaacaa aacaaaacat agccttcttt gtcgctatgg cttaacctca gcggctgctc
      121 ttttgctgac ctttggaggt gtaagcgcgg ttaaggcgga tgagcaaagt aatattgaag
      181 cactacagag tcaattacag gctttacgcg aaaatttgcc taaggtcgga gataatattc
      241 ctaagcatga tgttttcagg caacatgtgt tttataaaca tttggaagag tttgaagctc
      301 gactggattt ttacttaaaa acgttggatg ggagtctatc ccaactgcaa ggtccacgtg
      361 gtgaaaaagg agaccagggc ccacgtggtg aacaagggct acgaggcgaa atcggtcctg
      421 aaggcccaca aggcaaaacc ggcccagcag g
//
LOCUS       DAUCLR010000180          438 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.183, whole genome shotgun sequence.
ACCESSION   DAUCLR010000180 DAUCLR010000000
VERSION     DAUCLR010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.183"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
BASE COUNT          158 a           83 c           80 g          117 t
ORIGIN      
        1 ctctacttgc cgcttgtagc aagtagtcta gccattgtag gtcttgtata aaacaaaagc
       61 aagtgttcgc tggctgggct tgtagtagca gtaacagtat aatctgaacc gacagccatt
      121 ccctattaaa atagacgcaa atgtccacgc tttcactgta gttgctagta aggtctaaca
      181 caaaacatca aaggctttca aaaacaactc ctaagcgcaa aaaggcggac taacagtccc
      241 aagcaagcac tttctgaggt aataagagat tgccatgatt ttctaatcat ttgatgttta
      301 aaatgataaa aatatgaact tgaaatgaaa gaatgtgagt agtaaactag aaccgtatca
      361 aaaataatga aatggagtga ttttatgaaa acaaaatcaa aacgcttttt aaacctagca
      421 accctttgct tggcccta
//
LOCUS       DAUCLR010000181          428 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.184, whole genome shotgun sequence.
ACCESSION   DAUCLR010000181 DAUCLR010000000
VERSION     DAUCLR010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.184"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..>428
                     /gene="enn"
                     /locus_tag="VGT87_001826"
     CDS             <1..>428
                     /gene="enn"
                     /locus_tag="VGT87_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M-related protein Enn"
                     /protein_id="HEQ3814643.1"
                     /translation="LEAKHQKLEADHQALEAKHQKLAEEHQVSETSRKGLSRDLEASR
                     EANKKVTSELTQAKAQLLALEESKKLSEKEKAELQAKLDAQGKALKEQLAKQTEELAK
                     LRAEKAAGSKTPATKPANKERSGRAAQTATRPSQNKGMRS"
BASE COUNT          189 a           88 c           83 g           68 t
ORIGIN      
        1 ctagaagcta agcaccaaaa attagaggct gatcaccaag ccctagaagc taagcaccaa
       61 aaattagcag aagaacatca agtttcagag actagccgca agggtctaag ccgtgacctt
      121 gaagcatcac gtgaagctaa taagaaggtt acatctgagt taacacaagc aaaagctcaa
      181 ctcttagcgc ttgaagaaag taagaaatta tcagaaaaag aaaaagctga gttacaagca
      241 aaactagatg cacaaggaaa agccctcaaa gaacaattag caaaacaaac tgaagagctt
      301 gcaaaactaa gagctgaaaa agcagcaggt tcaaaaacac ctgctaccaa accagctaat
      361 aaagaaagat caggtagagc tgctcaaaca gctacaagac ctagccaaaa taaaggaatg
      421 agatcaca
//
LOCUS       DAUCLR010000182          397 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.185, whole genome shotgun sequence.
ACCESSION   DAUCLR010000182 DAUCLR010000000
VERSION     DAUCLR010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.185"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..287
                     /locus_tag="VGT87_001827"
     CDS             <1..287
                     /locus_tag="VGT87_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020905049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEQ3814644.1"
                     /translation="HLYREAPFAILKVDPISQEEYVLRGIIDAYFLFDDHIVLVDYKT
                     DKYKQPIELKKRYQQQLELYAEALTQTYKLPVTKRYLVLMGGGKPEIVEV"
BASE COUNT          136 a           57 c           79 g          125 t
ORIGIN      
        1 aacacttgta tcgagaagcg ccatttgcta ttttaaaagt tgaccctatt agtcaagaag
       61 aatatgtcct acgtggtatt atagatgcct actttttgtt tgatgatcat attgtattag
      121 tggactataa aaccgataaa tacaagcagc ccattgagtt aaaaaagcgt taccaacaac
      181 agttggagtt atatgcagaa gctctcactc aaacgtataa acttcctgtg actaagcgct
      241 atcttgtttt aatgggaggt ggaaagccag aaattgtcga agtttaaggt tgttgtgaca
      301 caaggggatt ttgcaaatga taatagtaga gttgaggatt ttacgaaata cttgaaaagc
      361 tattgacctt tgaataataa tacaatataa taagttt
//
LOCUS       DAUCLR010000183          357 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.186, whole genome shotgun sequence.
ACCESSION   DAUCLR010000183 DAUCLR010000000
VERSION     DAUCLR010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.186"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..>357
                     /locus_tag="VGT87_001828"
     CDS             <1..>357
                     /locus_tag="VGT87_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004818439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814645.1"
                     /translation="VDKLFIDEAHNYKNLFLHTKMRNVAGIGQSEAFKSSDMYMKCRY
                     MDEMTDGKGVVFATGTPISNSMTELYTMQRYLQYDDLKARGLEHFDAWASTFGETENT
                     FELSPEGTGYRQKTRFS"
BASE COUNT          127 a           53 c           74 g          103 t
ORIGIN      
        1 agtagataag ttatttatag acgaagctca taattacaaa aacttattct tgcataccaa
       61 gatgagaaat gttgctggta tcggtcaaag tgaagccttt aagtcttcgg atatgtatat
      121 gaaatgtagg tatatggacg aaatgacaga tggcaaggga gttgtatttg ctactggtac
      181 accaatatca aattcaatga cagagcttta tactatgcaa agataccttc aatatgacga
      241 tttaaaggca agaggattag aacattttga cgcttgggct tctacttttg gagaaacaga
      301 aaacaccttt gaattatctc cagaaggcac tggatatagg caaaagacaa gattttc
//
LOCUS       DAUCLR010000184          352 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.187, whole genome shotgun sequence.
ACCESSION   DAUCLR010000184 DAUCLR010000000
VERSION     DAUCLR010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.187"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(8..>352)
                     /locus_tag="VGT87_001829"
     CDS             complement(8..>352)
                     /locus_tag="VGT87_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1617 family protein"
                     /protein_id="HEQ3814646.1"
                     /translation="RGRAKLLAKVETKLSEYAKDEIDIIDQYVAKNDDDKWITDDKGN
                     PKIEDTSKLAELNDFLDELASEPIVIKGGEYSKRFIDFLEYLVESEDEFTSEEIVLID
                     NILEQFEESKGE"
BASE COUNT           96 a           82 c           51 g          123 t
ORIGIN      
        1 cgcataatta ttctccttta ctttcttcaa attgttccaa aatattatca attaagacga
       61 tttcctctga tgtaaactca tcttcagatt cgactaaata ttccaaaaaa tcgataaagc
      121 gcttagagta ctcgccacct ttgatgacga taggctcgct tgctaactcg tctaaaaagt
      181 cgttaagctc agctaacttt gaggtgtcct caatttttgg attacctttg tcatctgtaa
      241 tccacttgtc atcatcattt ttagcgacat attgatcaat aatatcgatc tcatctttgg
      301 catactcgct tagcttagtc tctacttttg caagcagctt agcgcgccca cg
//
LOCUS       DAUCLR010000185          352 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.188, whole genome shotgun sequence.
ACCESSION   DAUCLR010000185 DAUCLR010000000
VERSION     DAUCLR010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.188"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(8..>352)
                     /locus_tag="VGT87_001830"
     CDS             complement(8..>352)
                     /locus_tag="VGT87_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1617 family protein"
                     /protein_id="HEQ3814647.1"
                     /translation="RGRAKLLAKVVDKINEYAKDETDLIDMYAAKDKDDKFVIDEHKN
                     IKLADPAKLDELNDLLNELADEEIVIKGGEYSKRFIDFLEYLAESEDEFTAQEIIIVD
                     SILEQYEGSKGE"
BASE COUNT           82 a           79 c           62 g          129 t
ORIGIN      
        1 ctcatggtta ttctcctttg cttccttcgt attgctctaa gatgctgtca acaataatga
       61 tttcttgagc tgtaaactca tcttcagatt cggctaaata ttccaaaaaa tcgataaatc
      121 gcttggagta ctcaccccct ttaatcacaa tttcttcatc agctagctcg ttgagtaggt
      181 cgttgagctc gtcgagttta gcggggtctg ctagcttgat gtttttgtgc tcatcaatga
      241 caaatttgtc atctttatcc ttagctgcat acatatcaat taagtcggtc tcatcttttg
      301 catactcatt gattttatcg actacttttg cgagtagctt agcacgtccg cg
//
LOCUS       DAUCLR010000186          343 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.189, whole genome shotgun sequence.
ACCESSION   DAUCLR010000186 DAUCLR010000000
VERSION     DAUCLR010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.189"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
BASE COUNT          139 a           46 c           50 g          108 t
ORIGIN      
        1 aaaatagaat tttttctaac taaaaataat agcacaatct ttggcagcaa tctgaattgt
       61 ttttccataa aagatttaag cacttacgtt tgcttaaaat ataggttatg tgacgtgcgt
      121 gcaagatcag attataacaa aatagttcca aataagccta tcactgagaa agcatcacag
      181 aagggttaaa ctttttttgt attagcgttt tatcagtaca acctaacact tatttttata
      241 aaaaaataaa aaagctattg acaatcttat acaagataac gtatactaat aaagctgttg
      301 taaatatggt aaagcaatag atgctatcag gaaaaaatgt gaa
//
LOCUS       DAUCLR010000187          336 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.190, whole genome shotgun sequence.
ACCESSION   DAUCLR010000187 DAUCLR010000000
VERSION     DAUCLR010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.190"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(58..139)
                     /locus_tag="VGT87_001831"
     tRNA            complement(58..139)
                     /locus_tag="VGT87_001831"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(103..105),aa:Leu,seq:tag)
     gene            complement(145..217)
                     /locus_tag="VGT87_001832"
     tRNA            complement(145..217)
                     /locus_tag="VGT87_001832"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(182..184),aa:Lys,seq:ttt)
     gene            complement(249..321)
                     /locus_tag="VGT87_001833"
     tRNA            complement(249..321)
                     /locus_tag="VGT87_001833"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(286..288),aa:Asp,seq:gtc)
BASE COUNT           88 a           97 c           68 g           83 t
ORIGIN      
        1 accctctgat tacaaatcag atgctctacc aactgagcta agccggctaa tcttttatgc
       61 gggttaaggg acttgaaccc ccacgccgtt aagcgccaga tcctaaatct ggtgcgtctg
      121 ccaattccgc caaacccgct tttatgaccc gtactgggct cgaaccagtg acccattgat
      181 taaaagtcaa ttgctctacc aactgagcta acgagtcttt tcttcaaaaa atatgactac
      241 tctttgaacg gtcccgacgg gaatcgaacc cgcgatcttc gccgtgacag ggcgacgtga
      301 taaccgctac actacgggac ctgtattttc caatac
//
LOCUS       DAUCLR010000188          323 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.191, whole genome shotgun sequence.
ACCESSION   DAUCLR010000188 DAUCLR010000000
VERSION     DAUCLR010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.191"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..282
                     /locus_tag="VGT87_001834"
     CDS             <1..282
                     /locus_tag="VGT87_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEQ3814648.1"
                     /translation="EAVYLFSGSYPKFNKFYSPALLQEHAMLKAIHKGIKQYNFLGIT
                     GKFDGSDGVLRFKQNFNGFILQKPGTFRCYPFPIKYHFIRLAKKLLNRY"
BASE COUNT          102 a           64 c           49 g          108 t
ORIGIN      
        1 gaggccgtct acctcttttc aggatcttac cccaaattta ataaatttta tagcccagct
       61 ctattacaag agcatgctat gctcaaggca attcacaagg gcatcaaaca atataatttt
      121 cttggtatta ctggaaaatt tgatggttca gacggtgtct tgcgctttaa acagaatttc
      181 aatggcttta ttttgcaaaa accaggaaca tttagatgtt atccatttcc aatcaaatat
      241 catttcatta gattggcaaa aaagctatta aaccgctatt aggctaatgc atttgcctgt
      301 agaatcacct ttattctctc ata
//
LOCUS       DAUCLR010000189          319 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.192, whole genome shotgun sequence.
ACCESSION   DAUCLR010000189 DAUCLR010000000
VERSION     DAUCLR010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.192"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            215..>319
                     /locus_tag="VGT87_001835"
     CDS             215..>319
                     /locus_tag="VGT87_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEQ3814649.1"
                     /translation="MAFGENGPRKKTTFEKVTMVVVILMVLVTVGGLIA"
BASE COUNT          105 a           48 c           62 g          104 t
ORIGIN      
        1 tttgtgtcta acttttgggg tgcagttcac cagacgagga ggtttttatc tgtaacatta
       61 ttttgcaaat gctaatgtat catattcata tgaaattcac aagatacaat actcagtaat
      121 agcctacata gcgtaaatat tcatctgata tttgcctaaa acgatctttc atgttaagat
      181 ataagaagaa aaatcaatag atgaggtatt taacgtggca tttggagaaa atggacctcg
      241 caaaaaaaca acctttgaaa aagtgacaat ggtcgttgtt atcctaatgg ttcttgtaac
      301 tgttggcggg cttattgcg
//
LOCUS       DAUCLR010000190          318 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.193, whole genome shotgun sequence.
ACCESSION   DAUCLR010000190 DAUCLR010000000
VERSION     DAUCLR010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.193"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..216
                     /locus_tag="VGT87_001836"
     CDS             <1..216
                     /locus_tag="VGT87_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEQ3814650.1"
                     /translation="AAIGQKIVARSDIKALRKNVLAKCYGGDVSRKRKLLEKQKAGKK
                     RMKAIGSVEVPQEAFLSVLSMDDDTKK"
BASE COUNT          103 a           61 c           67 g           87 t
ORIGIN      
        1 gccgctattg gtcaaaaaat tgttgcgcgt tcagacatca aggcccttcg taaaaacgtt
       61 ttagcgaagt gttatggcgg tgacgtgtct cgtaaacgta aattgctcga aaaacaaaag
      121 gctggtaaaa aacggatgaa ggctattggt tctgtcgaag tccctcaaga agccttcttg
      181 agtgttcttt caatggatga cgataccaag aaataaccaa gcttaaccgc ttagtcttca
      241 gaggagaact cttgtgggtc tacatgacca aaaaattgat tatctcataa aaaaagaact
      301 ttacaatctc tttcagat
//
LOCUS       DAUCLR010000191          314 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.194, whole genome shotgun sequence.
ACCESSION   DAUCLR010000191 DAUCLR010000000
VERSION     DAUCLR010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.194"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            20..>314
                     /locus_tag="VGT87_001837"
     CDS             20..>314
                     /locus_tag="VGT87_001837"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEQ3814651.1"
                     /translation="MNSHHACQTLQVSRSGFYAYLKRRPSSRHVENEALKEMIKAIFY
                     EHKERYGSVRITQELCRRGIHVNHKRVGRLLHQLGLYAKGSRYQYKYYNRRRSS"
BASE COUNT           97 a           62 c           61 g           94 t
ORIGIN      
        1 gaaggaaaat agtgttagct tgaatagtca tcacgcctgt caaactcttc aagtctcccg
       61 ttcaggtttc tatgcttatt taaaacgtcg tccctcttca aggcatgttg agaatgaagc
      121 tttgaaggaa atgattaaag ccattttcta tgaacataaa gagcgttacg gaagtgtacg
      181 aattacccaa gaactttgta gacgtggcat ccatgtcaat cataaacgtg ttggtagact
      241 ccttcatcag ctaggtctct atgctaaagg aagtcgatat caatataaat actataaccg
      301 tcgacgttct tcat
//
LOCUS       DAUCLR010000192          311 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.195, whole genome shotgun sequence.
ACCESSION   DAUCLR010000192 DAUCLR010000000
VERSION     DAUCLR010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.195"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(<1..>311)
                     /locus_tag="VGT87_001838"
     CDS             complement(<1..>311)
                     /locus_tag="VGT87_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814652.1"
                     /translation="SIGITSSGDGIDSASSGRFCGVRFFRYAEGLQHTAKVDQAEIYG
                     DDIVFSDDFNIDRGFKMRPSLMPKMVDLNKMYQAILALGRCWLHANNTAWSWNFDTRS
                     A"
BASE COUNT           86 a           81 c           67 g           77 t
ORIGIN      
        1 attgcgctgc gtgtatcaaa attccacgac caagccgtgt tattagcatg cagccagcag
       61 cggccgagag ccaaaattgc ctggtacatc ttgtttaagt cgaccatttt tggcattagg
      121 ctaggccgca tcttaaagcc acgatcgatg ttaaaatcgt cgctaaagac aatatcatca
      181 ccataaattt cggcttgatc gacctttgct gtatgctgta acccttccgc gtaccggaaa
      241 aacctaactc cacagaaacg tccagacgaa gcgctgtcga tgccatctcc tgatgaggta
      301 atgccgatag a
//
LOCUS       DAUCLR010000193          302 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.196, whole genome shotgun sequence.
ACCESSION   DAUCLR010000193 DAUCLR010000000
VERSION     DAUCLR010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.196"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(28..>302)
                     /locus_tag="VGT87_001839"
     CDS             complement(28..>302)
                     /locus_tag="VGT87_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEQ3814653.1"
                     /translation="IVVLSFLGKNQALKDLGKSYQTLIVVNLLMNLFMPNVSMAGHIG
                     GVVGGALLSIVFPTKMRVITVKKTKRMLALVSYGIILVGVLVLGFL"
BASE COUNT          104 a           82 c           35 g           81 t
ORIGIN      
        1 gtaacagtgc taaccactca cttattttta taaaaaccca agcactaaca caccaaccaa
       61 aataatacca tagctcacta aagccaacat tcgcttggtt ttcttcactg ttatcactct
      121 catcttagtt ggaaaaacta tactcagtaa cgcaccaccc acgacaccgc caatatggcc
      181 tgccatactc acgtttggca taaaaagatt catcagtaaa ttgactacaa ttaacgtctg
      241 gtatgacttg cccaaatctt tcaacgcttg gttcttaccc aaaaaactca atactacaat
      301 ag
//
LOCUS       DAUCLR010000194          288 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.197, whole genome shotgun sequence.
ACCESSION   DAUCLR010000194 DAUCLR010000000
VERSION     DAUCLR010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.197"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..>288
                     /locus_tag="VGT87_001840"
     CDS             <1..>288
                     /locus_tag="VGT87_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HEQ3814654.1"
                     /translation="EMNEPLFATYVDLSEGCPSHDTLERVISLVNSDRLKELKVQFEQ
                     SLTSLDAVHQLISVDGKTIRGNRGKNQKPVHIVTAYDGGHHLSLGQVAVEE"
BASE COUNT           83 a           45 c           73 g           87 t
ORIGIN      
        1 tgaaatgaat gaaccattgt ttgcgaccta cgttgatttg agtgaaggtt gtccgtctca
       61 tgatacctta gagcgtgtga ttagtcttgt taattcagac cgtttaaaag agcttaaagt
      121 tcaatttgag caatcattga caagcttaga tgccgttcat caactgattt cagtggacgg
      181 taaaacgatt cgaggcaatc gaggtaaaaa tcagaagcct gttcatattg taacggctta
      241 tgatgggggt catcatctta gtttgggaca ggtagcggtt gaggagaa
//
LOCUS       DAUCLR010000195          267 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.198, whole genome shotgun sequence.
ACCESSION   DAUCLR010000195 DAUCLR010000000
VERSION     DAUCLR010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.198"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(3..76)
                     /locus_tag="VGT87_001841"
     tRNA            complement(3..76)
                     /locus_tag="VGT87_001841"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40..42),aa:Arg,seq:acg)
     gene            complement(93..177)
                     /locus_tag="VGT87_001842"
     tRNA            complement(93..177)
                     /locus_tag="VGT87_001842"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(141..143),aa:Leu,seq:taa)
     gene            complement(186..257)
                     /locus_tag="VGT87_001843"
     tRNA            complement(186..257)
                     /locus_tag="VGT87_001843"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(223..225),aa:Gly,seq:gcc)
BASE COUNT           69 a           78 c           61 g           59 t
ORIGIN      
        1 gatgcaccct agaggagtcg aacctctaac cgcctgattc gtagtcaggt actctatcca
       61 gttgagctaa gggtgctatt tcaaaaaatt aatgccgagg accggaatcg aaccggtacg
      121 atgtaaacca tcgcaggatt ttaagtcctg tgcgtctgcc agttccgcca ccccggctcc
      181 tcaaaagcga acgacgggat tcgaacccgc gacccccacc ttggcaaggt gatgttctac
      241 cactgaacta cgttcgcaac taatctc
//
LOCUS       DAUCLR010000196          266 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.199, whole genome shotgun sequence.
ACCESSION   DAUCLR010000196 DAUCLR010000000
VERSION     DAUCLR010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.199"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..264
                     /locus_tag="VGT87_001844"
     CDS             <1..264
                     /locus_tag="VGT87_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HEQ3814655.1"
                     /translation="MSGSNDATVIEEDTKPKRFFHFDNEPQAPEKPKEQPSLSLPQAP
                     VYKAAHHLPASGDKREASFTIVALTIIGAAGLLSKKRRDTEEN"
BASE COUNT           91 a           71 c           47 g           57 t
ORIGIN      
        1 atgtcaggct caaatgacgc tactgttatc gaagaagata cgaaaccaaa acgcttcttc
       61 cactttgata acgagccaca agcaccagaa aaacctaaag agcaaccatc tctcagctta
      121 ccacaagctc cagtctataa ggcagctcat cacttgcctg catctggaga caaacgtgaa
      181 gcatccttta caattgttgc tctaacaatt attggagctg caggtttgct cagcaaaaaa
      241 cgtcgcgaca ccgaagaaaa ctaacc
//
LOCUS       DAUCLR010000197          265 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.200, whole genome shotgun sequence.
ACCESSION   DAUCLR010000197 DAUCLR010000000
VERSION     DAUCLR010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.200"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            18..98
                     /locus_tag="VGT87_001845"
     tRNA            18..98
                     /locus_tag="VGT87_001845"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52..54,aa:Tyr,seq:gta)
     gene            105..175
                     /locus_tag="VGT87_001846"
     tRNA            105..175
                     /locus_tag="VGT87_001846"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:137..139,aa:Trp,seq:cca)
     gene            187..259
                     /locus_tag="VGT87_001847"
     tRNA            187..259
                     /locus_tag="VGT87_001847"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:220..222,aa:His,seq:gtg)
BASE COUNT           65 a           57 c           65 g           78 t
ORIGIN      
        1 aaaatttaat attttacgga aagatagcga agaggctaaa cgcggcggac tgtaaatccg
       61 ctccttcggg ttcgtgggtt cgaatccctc tctttccatc ctaaacgggc atagtttaaa
      121 ggtagaacta aggtctccaa aaccttcagt gtgggttcga ttcctactgc ccgtgttaga
      181 aatatggcgg gtgtggtgaa gtggttaaca catcagattg tggctctgac attcgtgggt
      241 tcgattccca tcactcgcct atttt
//
LOCUS       DAUCLR010000198          250 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.201, whole genome shotgun sequence.
ACCESSION   DAUCLR010000198 DAUCLR010000000
VERSION     DAUCLR010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.201"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            1..188
                     /locus_tag="VGT87_001848"
                     /pseudo
     CDS             1..188
                     /locus_tag="VGT87_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IgA receptor"
BASE COUNT          118 a           52 c           46 g           34 t
ORIGIN      
        1 tagccgaagc aaatagcaaa cttcaagccc ttgaaaaact aaacaaagag cttgaagaag
       61 gtaagaaatt atcagaaaaa gaaaaagctg agttacaagc aagactagaa gcagaagcaa
      121 aagctcttaa agagcaattg gctaaacaag ctgaagaact tgccaaacta aaaggcaacc
      181 aaacaccaaa cgctaaagta gccccacaag ctaaccgttc aagatcagca atgacgcaac
      241 aaaagagaac
//
LOCUS       DAUCLR010000199          248 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.202, whole genome shotgun sequence.
ACCESSION   DAUCLR010000199 DAUCLR010000000
VERSION     DAUCLR010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.202"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..128
                     /locus_tag="VGT87_001849"
     CDS             <1..128
                     /locus_tag="VGT87_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator"
                     /protein_id="HEQ3814656.1"
                     /translation="KRQLETGVEKMKQAIQVLDILNEDKLKLHYADHFDKLVNKD"
BASE COUNT           96 a           46 c           34 g           72 t
ORIGIN      
        1 ttaagcgcca attagaaact ggagttgaaa agatgaaaca agccatacaa gttcttgata
       61 ttttaaatga agacaagtta aaattacatt acgctgacca ttttgataaa ctagtcaaca
      121 aagattaagt aaaatcattc tttacctttt aacgaaacgc caatcaaggg tgtctcaata
      181 aaaaatcatc tactctgaca acttttctct tttacctatc gacagctaaa aagccttgga
      241 aactgcta
//
LOCUS       DAUCLR010000200          240 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.203, whole genome shotgun sequence.
ACCESSION   DAUCLR010000200 DAUCLR010000000
VERSION     DAUCLR010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.203"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..159
                     /locus_tag="VGT87_001850"
     CDS             <1..159
                     /locus_tag="VGT87_001850"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HEQ3814657.1"
                     /translation="IDVYSRKIVGWAMGRRMQDKLVTEAFNQAYNREKPKEGVIVHTD
                     QGSQYTGA"
BASE COUNT           87 a           38 c           58 g           57 t
ORIGIN      
        1 atagacgttt atagtagaaa gattgttggt tgggcaatgg gccgacgtat gcaagacaag
       61 ttggtaacag aggctttcaa tcaagcttat aatagagaaa agcctaaaga aggggtaatc
      121 gtccatacag atcagggttc tcaatatacc ggagcatgat ttcaggacct acttagacag
      181 aaaaaatgta agtccagtat gagtcgaaaa ggtaatccat acgataatgc actcatggaa
//
LOCUS       DAUCLR010000201          240 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.204, whole genome shotgun sequence.
ACCESSION   DAUCLR010000201 DAUCLR010000000
VERSION     DAUCLR010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.204"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..159
                     /locus_tag="VGT87_001851"
     CDS             <1..159
                     /locus_tag="VGT87_001851"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HEQ3814658.1"
                     /translation="IDVYSRKVVGWAMGRRMQDKLVTEAFNQAYNREKPKEGGIVHTD
                     QGSQYTGA"
BASE COUNT           85 a           37 c           62 g           56 t
ORIGIN      
        1 atagacgttt atagtagaaa ggttgttggt tgggcaatgg gccgacgtat gcaagacaag
       61 ttggtaacag aagctttcaa tcaagcttat aatagagaaa agcctaaaga agggggaatc
      121 gtccatacag atcagggttc tcaatatacc ggagcatgat ttcaggacct gcttagatgg
      181 aaaaaatgta agtccaggat gagtcgaaaa ggtaatccat acgataatgc actcatggaa
//
LOCUS       DAUCLR010000202          227 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.205, whole genome shotgun sequence.
ACCESSION   DAUCLR010000202 DAUCLR010000000
VERSION     DAUCLR010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.205"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..122
                     /locus_tag="VGT87_001852"
     CDS             <1..122
                     /locus_tag="VGT87_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEQ3814659.1"
                     /translation="TAAIFLTNRTYDEDDKSKWLKERQLLYNALKHDLTTPVS"
BASE COUNT           76 a           36 c           43 g           72 t
ORIGIN      
        1 aaacggcagc tatttttttg accaaccgta cttacgacga ggatgataaa agtaaatggt
       61 taaaagaacg tcagttgctt tacaatgcac taaagcatga tctgacaaca cccgtatcgt
      121 aacgtctaaa ggggcagttt tagtgatctt attactgttt ttaaagtttc ttctaaaaaa
      181 tagccatttc atttgtctga tgcgattaga gtgattataa gtgaaaa
//
LOCUS       DAUCLR010000203          226 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.206, whole genome shotgun sequence.
ACCESSION   DAUCLR010000203 DAUCLR010000000
VERSION     DAUCLR010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..226
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.206"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..>226
                     /locus_tag="VGT87_001853"
     CDS             <1..>226
                     /locus_tag="VGT87_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEQ3814660.1"
                     /translation="DALEKEKEDLKTTLAKTTKENEISEASRKGLSRDLEASRAAKKE
                     LEAKHQKLEAENKKLTEANQISEASRKGLS"
BASE COUNT          102 a           35 c           44 g           45 t
ORIGIN      
        1 ttgatgcttt agaaaaagaa aaagaagatc tcaaaacaac tttagctaag actactaaag
       61 aaaatgagat ttcagaagct agccgtaaag ggttaagccg agacttagaa gcttctcgtg
      121 cagctaaaaa agagctagaa gctaagcatc aaaaattaga agcagaaaac aaaaaactaa
      181 cagaagccaa tcagatttca gaagctagtc gtaaaggtct aagtaa
//
LOCUS       DAUCLR010000204          222 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.207, whole genome shotgun sequence.
ACCESSION   DAUCLR010000204 DAUCLR010000000
VERSION     DAUCLR010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.207"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            82..155
                     /locus_tag="VGT87_001854"
     tRNA            82..155
                     /locus_tag="VGT87_001854"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:116..118,aa:Met,seq:cat)
BASE COUNT           52 a           48 c           65 g           57 t
ORIGIN      
        1 ggctgaaggg aacggtcttg aaaaccgtca ggcgtgtaaa agcgtgcgtg ggttcgaatc
       61 ccacatcctc cttttaacta tcgcgggatg gagcagttag gtagctcgtc gggctcataa
      121 cccgaaggtc gtaggttcaa atcctgctcc cgcaatacat aagtatgtag ttgttggctc
      181 ggtagctcag ttggtagagc aatggattga agctccatgt gt
//
LOCUS       DAUCLR010000205          218 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.208, whole genome shotgun sequence.
ACCESSION   DAUCLR010000205 DAUCLR010000000
VERSION     DAUCLR010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..218
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.208"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..194
                     /locus_tag="VGT87_001855"
     CDS             <1..194
                     /locus_tag="VGT87_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEQ3814661.1"
                     /translation="ATEMRDHPDFKQFVLNPTHKIVFIDYEGFRLFVKWKSRNRYKPK
                     KETLVEMLENIKFEKRVGV"
BASE COUNT           85 a           31 c           40 g           62 t
ORIGIN      
        1 gggcgacaga aatgcgtgat catccagact ttaaacagtt tgtattaaat ccaacgcata
       61 aaatcgtatt cattgattac gaaggtttcc gtttatttgt taaatggaaa agtcgtaacc
      121 gctataaacc caagaaggaa accctggtag aaatgcttga aaatattaaa ttcgaaaaaa
      181 gagtaggagt ataacatgaa caaattagaa ttattttt
//
LOCUS       DAUCLR010000206          216 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.210, whole genome shotgun sequence.
ACCESSION   DAUCLR010000206 DAUCLR010000000
VERSION     DAUCLR010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.210"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..>216
                     /locus_tag="VGT87_001856"
     CDS             <1..>216
                     /locus_tag="VGT87_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEQ3814662.1"
                     /translation="NPLETTRLIIFAPGKLDSKRRLVKLLKRDALVLEASPLKEAELR
                     TYFQKYSHQLGLGFESGAFEQLLLKSN"
BASE COUNT           68 a           39 c           45 g           64 t
ORIGIN      
        1 aaatccctta gagactactc gactaattat ctttgctcca ggtaaattgg atagtaagag
       61 acggcttgtt aagcttttga aacgtgatgc ccttgtttta gaagccagcc ctctgaaaga
      121 agcagagcta agaacttatt ttcaaaaata cagtcatcaa ctgggtttgg gatttgagag
      181 tggcgctttt gaacaactgc ttttaaaatc aaacga
//
LOCUS       DAUCLR010000207          205 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.denovo.212, whole genome shotgun sequence.
ACCESSION   DAUCLR010000207 DAUCLR010000000
VERSION     DAUCLR010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.denovo.212"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            <1..183
                     /locus_tag="VGT87_001857"
     CDS             <1..183
                     /locus_tag="VGT87_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HEQ3814663.1"
                     /translation="DHHQTLDKRAAFNLNLIRKMCLYFLKVMVFPKKDLSYRRKQRYI
                     SVHLEDYLVQLFGERG"
BASE COUNT           72 a           32 c           37 g           64 t
ORIGIN      
        1 gatcatcatc agacattgga taaaagagcc gcatttaacc taaatcttat ccgaaaaatg
       61 tgcttatatt ttctcaaagt gatggtattt cctaaaaaag acctcagtta tcgtcgcaaa
      121 caacggtata tttctgtcca tttggaagat tatttagtcc aattatttgg agaaagaggc
      181 taagcagatg attgatttaa aaagg
//
LOCUS       DAUCLR010000208         7741 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.guided.084, whole genome shotgun sequence.
ACCESSION   DAUCLR010000208 DAUCLR010000000
VERSION     DAUCLR010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7741
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.guided.084"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            77..946
                     /locus_tag="VGT87_001858"
     CDS             77..946
                     /locus_tag="VGT87_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEQ3814664.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            927..1661
                     /locus_tag="VGT87_001859"
     CDS             927..1661
                     /locus_tag="VGT87_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEQ3814665.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1694..2602
                     /locus_tag="VGT87_001860"
     CDS             1694..2602
                     /locus_tag="VGT87_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HEQ3814666.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2599..3141
                     /gene="sat4"
                     /locus_tag="VGT87_001861"
     CDS             2599..3141
                     /gene="sat4"
                     /locus_tag="VGT87_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HEQ3814667.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            3234..4028
                     /locus_tag="VGT87_001862"
                     /note="aph(3')-IIIa"
     CDS             3234..4028
                     /locus_tag="VGT87_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEQ3814668.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            4304..4876
                     /locus_tag="VGT87_001863"
     CDS             4304..4876
                     /locus_tag="VGT87_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEQ3814669.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
     gene            4849..5256
                     /locus_tag="VGT87_001864"
     CDS             4849..5256
                     /locus_tag="VGT87_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEQ3814670.1"
                     /translation="MVLNRFCRLRNEYRNFRVDRIKSICIEEELCQSHDGSLEQILKQ
                     MLSYKKLYNVILRAEKGETYNSIKNRYSLGFLEETDLGSKMEIEFQTDSFEILSKQLI
                     EYGSGIEIVQPDELKCITRKHLAQITNHCLNLI"
     gene            5367..5729
                     /locus_tag="VGT87_001865"
                     /pseudo
     CDS             5367..5729
                     /locus_tag="VGT87_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014636358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibD"
     gene            5725..6393
                     /locus_tag="VGT87_001866"
                     /pseudo
     CDS             5725..6393
                     /locus_tag="VGT87_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSsu5 family transposase"
     gene            complement(6768..>7741)
                     /locus_tag="VGT87_001867"
     CDS             complement(6768..>7741)
                     /locus_tag="VGT87_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HEQ3814671.1"
                     /translation="TLCQLYAKQNAQRPKVRKNTETGRKYLMDILKKDKLGARSIDSI
                     KPSDAKEWAIRMSENGYAYQTINNYKRSLKASFYIAIQDDCVRKNPFDFQLNAVLDDD
                     TVPKTVLTGEQEEKLLAFAKADKTYSKNYDEILILLKTGLRISEFGGLTLPDLDFENR
                     LVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVEEAYQAFKRVLANRKNDKRVEIDGY
                     SDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNEDKLPHITPHSLRHTFCTNYANAGM
                     NPKALQYIMGHANIAMTLNYYAHATFDSAMAEMKRLNKEKQQERLVA"
BASE COUNT         2533 a         1258 c         1652 g         2298 t
ORIGIN      
        1 tcaagtttgt gcaggcattt attttaccag atggagaaga aataaaaata tctaacgaat
       61 aaaggaggat gtttatatgg taggtaatgt tattcaaata gtaacagaga aattatcatc
      121 tttgcctttt atagaaggca tcgtattagg gggctcacgt gcaagaggca cccatacaga
      181 aaattctgat atagatatcg gaatctatta caattcagac tcatttgacc tgacagcgat
      241 taatcaaatt gctacagaat tggatgataa aaatagaaac aaccttgttg tgcctcccgg
      301 agcgtggggt gattggatta atggcggcgg atggttattt ataaatgggt atcatgttga
      361 cttgatttta cgtgatataa aacgggtaga acaaataatc aaagatacgg agcaaggaat
      421 tgttactgcc aattatcaga ccgggcatcc ccatggttat ataagtgcaa tgtatcgtgg
      481 agaattagcg attagtaaaa tactatatgc taagaacgaa agcttatgcg aattaaaaaa
      541 gcaggcagaa atttacccta ctgctctaaa gaaaagcttg atgaactttt ttatatttga
      601 agctgaattc tctttaatgt ttgtaaaagc aaatgcgggg gtagaggata aatattatat
      661 tgcaggccat gtttttcgta ttatttcatg cttaaatcaa gtactatttg catgtaataa
      721 tgcttattgc attaacgaaa agaaagctat aaaactgctt gaaacttttg aatataaacc
      781 tgaaaaatat gccgaaagag ttaatcatat ttttgaagta ctcggtcttt ctctttttga
      841 atgctacgat atgaccgaga aactttataa agaagtgaat gaaattgtat cggagataaa
      901 taacttttta aacgaggaga gttcagatga aagaaaacaa atatgatgat aatatatttt
      961 ttcaaaaata cagtcaaatg agtcgctcgc agaaaggact ggctggtgcg ggagaatggg
     1021 agactttgaa aaagatgcta cctgatttta agggtaagcg tgtgcttgat ttaggatgcg
     1081 gctatggatg gcactgtata tatgcgatgg aaaacggtgc ttcctctgta gtaggtgttg
     1141 atatttctca taaaatgctc gaagtagcaa aaggaaaaac ccattttcca cagattgaat
     1201 atgaatgctg tgccatagaa gatgtggatt tcccagagga gagctttgat gtaatactaa
     1261 gttcgcttgc gtttcattat gtagcagact atgagaattt aataaaaaag atatatagga
     1321 tgctgaaggc tggtggcaat ttagttttta cagttgaaca tcctgttttt actgctcatg
     1381 gaacacaaga ctggtattat aacgaaaaag gagaaatact gcatttcccg gtggacaatt
     1441 attattatga gggcaaacgg acagctatgt ttttggaaga aaaggttaca aaatatcata
     1501 gaacactgac cacatatcta aatacactgc tttcaaatag ttttataata aatcagattg
     1561 tggagccaca gccgccagag aacatgatgg atattccggg gatggcggat gaaatgcgac
     1621 gcccaatgat gctgattgta tcggcaaaaa agaagatgta ataatataga aaaaataaac
     1681 gaggagtatg taaatgagat cagaaaaaga aatgatggat ttagtacttt ctttagcaga
     1741 acaggatgaa cgtattcgaa ttgtgaccct tgaggggtca cgcgcaaata ttaatatacc
     1801 taaagatgaa tttcaggatt atgatattac atattttgta agtgatatag aaccgtttat
     1861 atctaatgat gactggctta atcaatttgg gaatataata atgatgcaaa agccggagga
     1921 tatggaatta ttcccacctg aagaaaaggg attttcctat cttatgctat ttgatgatta
     1981 caataaaatt gatcttacct tattgccctt ggaagagtta gataattacc taaagggcga
     2041 taaattaata aaggttctaa ttgataaaga ttgtagaatt aaaagggaca tagttccgac
     2101 tgatatagat tatcatgtaa gaaagccaag cgcaagggag tatgatgatt gctgcaatga
     2161 attttggaat gtaacacctt atgttattaa aggattgtgc cgtaaggaaa ttttatttgc
     2221 tattgatcat tttaatcaga ttgttcgcca tgagctgctg agaatgatat catggaaggt
     2281 cggcatcgaa acaggcttta aattaagtgt aggcaagaac tataagttta ttgaaaggta
     2341 tatatccgag gatttgtggg agaaactttt gtccacctac cggatggatt cctatgaaaa
     2401 catatgggaa gcattatttc tatgccatca attgttcagg gcggtatccg gtgaggtggc
     2461 ggaaaggctt cattatgcct atccggagta tgataggaat ataacaaaat ataccaggga
     2521 catgtataaa aaatacactg gtaaaaccgg ctgcctggat agcacatatg ccgctgatat
     2581 agaagagagg cgggaacagt gattacagaa atgaaagcag ggcacctgaa agatatcgat
     2641 aaacccagcg aaccatttga ggtgataggt aagattatac cgaggtatga aaacgagaat
     2701 tggaccttta cagaattact ctatgaagcg ccatatttaa aaagctacca agacgaagag
     2761 gatgaagagg atgaggaggc agattgcctt gaatatattg acaatactga taagataata
     2821 tatctttact accaagacga taaatgcgtc ggaaaagtta aactgcgaaa aaattggaac
     2881 cggtacgctt atatagaaga tatcgccgta tgtaaggatt tcagggggca aggcataggc
     2941 agcgcgctta tcaatatatc tatagaatgg gcaaagcata aaaacttgca tggactaatg
     3001 cttgaaaccc aggacaataa ccttatagct tgtaaattct atcataattg tggtttcaaa
     3061 atcggctccg tcgatactat gttatacgcc aactttgaaa acaactttga aaaagctgtt
     3121 ttctggtatt taaggtttta gaatgcaagg aacagtgaat tggagttcgt cttgttataa
     3181 ttagcttctt ggggtatctt taaatactgt agaaaagagg aaggaaataa taaatggcta
     3241 aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa ataccgctgc gtaaaagata
     3301 cggaaggaat gtctcctgct aaggtatata agctggtggg agaaaatgaa aacctatatt
     3361 taaaaatgac ggacagccgg tataaaggga ccacctatga tgtggaacga gaaaaggaca
     3421 tgatgctatg gctggaagga aagctgcctg ttccaaaggt cctgcacttt gaacggcatg
     3481 atggctggag caatctgctc atgagtgagg ccgatggcgt cctttgctcg gaagagtatg
     3541 aagatgaaca aagccctgaa aagattatcg agctgtatgc ggagtgcatc aggctctttc
     3601 actccatcga catatcggat tgtccctata cgaatagctt agacagccgc ttagccgaat
     3661 tggattactt actgaataac gatctggccg atgtggattg cgaaaactgg gaagaagaca
     3721 ctccatttaa agatccgcgc gagctgtatg attttttaaa gacggaaaag cccgaagagg
     3781 aacttgtctt ttcccacggc gacctgggag acagcaacat ctttgtgaaa gatggcaaag
     3841 taagtggctt tattgatctt gggagaagcg gcagggcgga caagtggtat gacattgcct
     3901 tctgcgtccg atcgatcagg gaggatatcg gggaagaaca gtatgtcgag ctattttttg
     3961 acttactggg gatcaagcct gattgggaga aaataaaata ttatatttta ctggatgaat
     4021 tgttttagta cctagattta gatgtctaaa aagctttaac tacaagcttt ttagacatct
     4081 aatcttttct gaagtacatc cgcaactgtc catactctga tgttttatat cttttctaaa
     4141 agttcgctag atagagttct atattagaga tgtaaagagt tttggtgaag aggtttgtga
     4201 gtatggcttt tataaccagg gtgcaatgag aagcactaac agcagctagc ttaagaacgc
     4261 ataggatata ctttttatgg aagtcacaga ggaggaataa aatttgaaca ggattaacag
     4321 agtaacctct attcttattc agctgcaatc aaaaaaaata attcctgcca aagaaattgc
     4381 acagcgattt aatataagct taaggacagt ttacagagat atccggacac ttgaagaagc
     4441 cggaatacca attggatctg aggccggaaa aggatatttt cttgtagagg gcttcctact
     4501 tccgccggta atgtttacag cggcggaagt gggtgcattg attacagcag ggaaattttt
     4561 aaattgccat ggagatgaat catttataaa ggattttgat tcagctatgt ataaaatcaa
     4621 atctattttg aagcatggtg aaaaaaacta tgcacaggag ttggagaaca gtattaacgt
     4681 gtacagcaca tctggacaga aaaatacatt ggcggataac gttattgcag caattcagac
     4741 tgcaatctgc aataaaaggg taatatcaat tcaatatcct gcatcaggag ggcaagaacc
     4801 ggaaagcaga atgatagagc ctatatcact ggggttttat gaacagaact ggtacttaat
     4861 cggttttgca ggctaagaaa tgaatatagg aattttcgtg tggatcggat taaaagcatt
     4921 tgcatagaag aagagctctg tcaaagccat gatggctctt tggaacaaat attaaaacaa
     4981 atgctttcat ataaaaaact atataatgta attcttagag ctgaaaaagg ggagacatac
     5041 aattctatca agaacaggta ttctctcggc ttccttgaag aaaccgattt gggaagtaaa
     5101 atggaaatcg aattccagac agattccttt gagatattga gcaagcagct gattgaatat
     5161 ggatcaggca ttgaaatagt ccaacctgat gaattaaaat gcataacccg taaacacctg
     5221 gcacaaatta cgaatcactg cctaaacttg atttagagaa agcgattggt ctgttatgta
     5281 ttttttgtaa ggtttcatat cccctgctga catacagctg tcagtgtatt atgatattct
     5341 actatcaaaa aggaggattg aaaaatatga aaccaaagat aattatgcac acacaaatta
     5401 gccttgatgg ccgcattaaa ggctttgata atccggaagt ttactaccag gttgccgggg
     5461 gaattcattc ggatgcggtg ctgtttgggt ccaatactgt ttttactgca tttgaaaaat
     5521 atcctgctga aacagaggct gattttgaga aaatcataac aagcccggag gaccccagac
     5581 caataggagt catacctgac agcagaggta tcttgagaag tcttcattgc ttaagaaatc
     5641 ttggatatct gaaggaaatt gtcattctgg tatcaactgc cactccgaag gaatatttga
     5701 attatttgga ggaaaggcat tacccatacc gagatattgg ttcggggtga cagcggcttc
     5761 gccacaccag aggtgtatga gtcttgtgaa gcaactgaaa gccagtacgt tatccgatta
     5821 aagagcaatc ggaggttaag tcagttagct gaacattctg ttctttacgg ggacaatcaa
     5881 gaatgggaag agcgagaagt gcagtatttt tcactacctt atcaagcaca atcctggtcg
     5941 aaacctcgtc gtgtctgtat acgctccgtc cgtgaaggag gagaattgct atttcatcat
     6001 gcgtttattg tgactaatct atccgacaat gtctcgcctg aagtcatatt ttctctttac
     6061 ggcaagcgag gaacaatgga aaatttcatc aaagaagcga aaggtggctt ttacttcgat
     6121 aaaacagata gcccgcgttt cttagaaaat catgtcagaa tgatgatcag tgtccttgca
     6181 tacaacctcg ttaatttttt aaaaagcatt ggttttgata aagtgaacca gggcatgacg
     6241 attcattcca tccggctgat attgcttaaa gtagccggta aacttgttca gactgggaga
     6301 caggtctatc tcaaactgtc aagttatcat gtgtatcaaa ctgaatttta taaggttttt
     6361 gaacgcctgc ggcgatccaa acaatggatt tagactagtt atcacacaaa ttttttaacc
     6421 gattcttttt caagggattc gtctgccctt aaatagacaa ataattttta ttatgatctt
     6481 cttccgtagg catcctgttc gaaaacacat tattttggaa gaaagtatct gcctaattta
     6541 aaaattggca atttgttcaa aaaaacaggt taaatttaga gctatgaatt attcaggaaa
     6601 gtataaattt ctaattatct ttttatattt tcttaaatgc tcgtaaagcc ttattctatg
     6661 tgctttcgag tatttttact gtaggaagat acttcacgtt tctttgcata tttcctcatg
     6721 tcttagctgt cagaagtggt aaataagtag taaattcatt tgtactacta agcaacaaga
     6781 cgctcctgtt gcttctcttt attcaagcgt ttcatttctg ccattgcaga atcgaatgtt
     6841 gcatgtgcgt aatagttcag cgtcatggct atattagcat gtcccataat gtactgtaat
     6901 gcctttggat tcattcctgc atttgcatag ttggtacaga atgtatgtcg caaactatgt
     6961 ggagtgatgt gtggcaattt atcctcgtta tacttgttgt atttcttaac aagacctttc
     7021 atcataccat tatagtcact cgccactttt ggatagttct ttctattaag aaagaggaaa
     7081 tcactatatc catcaatctc aacacgctta tcattctttc gattcgctaa cactcgctta
     7141 aatgcttgat aggcttcttc aaccatagga acttgacgtt caccactttt ggtctttggt
     7201 gtttcaatgt agtacccaat ttcagtatct ctcaatagct gatggtctat attgacaaga
     7261 cgattctcaa aatctaaatc tggaagtgtc aaaccaccaa actctgaaat acgaagacct
     7321 gtttttaaga gtatcagaat ttcatcataa tttttgctgt aggttttatc agctttcgca
     7381 aaggctaaca gtttttcttc ctgttctcct gttagtacgg tcttagggac agtatcatca
     7441 tcaagaactg cattcagttg aaagtcaaat ggattcttcc gaacacaatc atcttgtatc
     7501 gcaatgtaga atgaagcctt taaagaacgt ttatagttat tgatggtttg ataggcataa
     7561 ccattttcac tcattctaat cgcccattct ttagcgtctg atggtttaat actatcaata
     7621 cttcttgcac ctaacttgtc tttcttcaaa atatccataa gatatttgcg tccagtttca
     7681 gtattttttc taacctttgg tctttgagcg ttctgttttg cgtaaagctg gcagagtgtc
     7741 a
//
LOCUS       DAUCLR010000209         3990 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.guided.114, whole genome shotgun sequence.
ACCESSION   DAUCLR010000209 DAUCLR010000000
VERSION     DAUCLR010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.guided.114"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            complement(4..168)
                     /locus_tag="VGT87_001868"
     CDS             complement(4..168)
                     /locus_tag="VGT87_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ3814672.1"
                     /translation="MYQDERKLDFKPLGIAIKKAREAKGWTQEYLAQLVDLTPRSIMG
                     SVAKFPISSF"
     gene            205..531
                     /locus_tag="VGT87_001869"
     CDS             205..531
                     /locus_tag="VGT87_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEQ3814673.1"
                     /translation="MIFERSMILCLVVYNFVLVERNFLLFVIPYIIIIYSLRKEVDIM
                     DYISVKAASEKWGISERRIQKLCEENRIDGTEKFGRAWMIPKDAEKPVDGRMKTRNKQ
                     EENHGI"
     gene            521..1135
                     /locus_tag="VGT87_001870"
     CDS             521..1135
                     /locus_tag="VGT87_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEQ3814674.1"
                     /translation="MEFNEKLQQLRTGKNLTQEQLAEQLYVSRTAISKWESGKGYPNI
                     ESLKCISKFFSVTIDELLSGEELITLAETENRSNLKKIYNYIYGILDMMAVAFIFLPL
                     YGNSVGGYVYAVNLLSFTATTPFNLAVYWSAFAALIVIGIAKIILTHLDKETWGGIAT
                     KCSLGLTALAICFLAAAREPYITALMFLLFVAKIVVWIKQNGTK"
     gene            1318..2235
                     /locus_tag="VGT87_001871"
     CDS             1318..2235
                     /locus_tag="VGT87_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEQ3814675.1"
                     /translation="MDYIIETENLTKRYGTATVVDKVNLHVPTGKIYGLLGRNGAGKT
                     TAMKMMLQLALPTDGTVRLFGTNYKENIHTLYSKVGSIIETPGFYSNLTGYENLQILA
                     KLRGGVSKSGVEKALEVVGLHKEKRKVFSDYSLGMKQRLGIAAAIMHEPELLILDEPI
                     NGLDPIGIVEIRSFLSELSHNHGITIFISSHVLSEIEQIADIIGVMHEGHLVEEVNIS
                     ELHKRNRKYIQFDLSDSEIAGKILESHYHMTDFTVQDGTVRIYDFSQSVGEINREFAR
                     NGLLVTRIDDSEENLEDYFSKLIGGGGIA"
     gene            2228..2977
                     /locus_tag="VGT87_001872"
     CDS             2228..2977
                     /locus_tag="VGT87_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEQ3814676.1"
                     /translation="MLNLISCELLKLKRSKMVLISVAGVLSTPLLMLIEALQTHFDKP
                     EIIFTLSDIYSDSVLYIMLLVNIMIYVAIAAYLFSREYTESTLKTILPIPISRTKLLI
                     GKFCTLLLWIVMLTLVTWAGIFIACGLYHAVFTLEGYSLLVAIVWLPKFLFGSILMFL
                     TVSPFVFVAMKTKGFVAPMIGSAVIVMGSAALSNQEWGALYPWTATYFLVQGKLQSTG
                     YPTLLSVSIIILVSAVGFLMTFHHFKKEDLK"
     gene            2977..3807
                     /locus_tag="VGT87_001873"
     CDS             2977..3807
                     /locus_tag="VGT87_001873"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEQ3814677.1"
                     /translation="MVLDFIEILKVIFLGIVEGITEWLPISSTGHMILADKFITLNMS
                     EAFKEMFFVVIQLGAILAVVVMFWNKMFPFQLKDKSQPLVKKDTFSLWFKVAVACIPS
                     GIMGILFDDYLDAYLQTPIVISIMLIFYGLLFILIENWNKKRTPTTMALSDISYKTAI
                     LIGAFQVLSLIPGTSRSGATIIGALLIGVSRVAAAEFTFFLAVPTMLGASAFKLLKFG
                     FDFTSAELLALVLGMAVAFAVSVLVIKFLMNFIKKHDFKVFGWYRIVLGILVVLITVM
                     "
BASE COUNT         1203 a          700 c          846 g         1241 t
ORIGIN      
        1 acctcaaaat gagcttatcg gaaactttgc aacagaaccc ataatagagc gtggcgtaag
       61 gtctaccagt tgggcaagat attcctgtgt ccaccctttt gcttcccgag ctttttttat
      121 cgctatacct aacggcttaa aatcaagttt tctttcatct tggtacattt taatatcacc
      181 ctatatcatt gtacatttcg ggtgatgata tttgaacgaa gtatgatttt atgtttagta
      241 gtgtataatt tcgtattagt ggagagaaac tttctattat tcgtcattcc gtatataata
      301 attatatact ctttgcgaaa ggaagttgat attatggatt acatttctgt gaaagccgct
      361 tctgaaaaat ggggcatatc ggaaagacgg atacaaaaat tatgtgagga aaatcgcatt
      421 gacggaactg aaaaatttgg tcgtgcatgg atgataccaa aagatgcaga aaaacccgtt
      481 gatggacgta tgaaaacaag gaacaaacag gaggagaatc atggaattta atgaaaagct
      541 acaacagctt aggactggaa agaacttaac gcaggaacaa cttgcggagc aattatatgt
      601 atcaagaaca gccatttcaa aatgggaaag cggcaagggt taccctaaca ttgagtcgct
      661 caagtgtatt tctaaattct tttctgtgac catagatgaa ctactatcgg gcgaagaact
      721 gattacactt gccgaaactg aaaatcgttc caacttgaaa aagatttaca actatattta
      781 tggaatattg gatatgatgg cagttgcgtt tatatttctt ccgttatatg gaaactctgt
      841 tggcggttat gtttatgcgg tcaatctact atcatttaca gccaccacac cattcaatct
      901 tgcagtctac tggagtgctt ttgccgcctt gattgtaata gggattgcta aaataatact
      961 tacccattta gataaagaaa catggggcgg cattgctaca aaatgctccc ttggactcac
     1021 tgctttggct atctgtttct tagcagcagc aagagaacca tatataacag cacttatgtt
     1081 tctgctgttt gtcgcaaaaa tagttgtttg gattaagcag aacggaacaa agtaaaaacg
     1141 atataaatcc cttgaaatag gctctgacac gataagtgtc ggggcttttt ttcatgttga
     1201 catgatagat gtaggcgatg tgacggtagg tttcttgact tttgatatag ctcctgtgat
     1261 aataaaattg tggtcagtga aaacaagaac agcaaccaac ggaaaggaaa atcaaatatg
     1321 gattatatca ttgaaacaga aaatcttacg aagcgatacg gaacagccac cgttgtcgat
     1381 aaggtcaatc ttcatgtgcc aacgggcaaa atttatggtt tgctcggcag aaacggagca
     1441 ggcaaaacca cagcaatgaa aatgatgttg cagcttgctc tcccaacgga tggaacggta
     1501 cgcttgtttg gcacaaacta taaggaaaat atccataccc tttatagcaa agtaggctct
     1561 attatcgaaa caccgggatt ttacagtaat ttaacagggt atgagaattt gcaaattctc
     1621 gctaaactgc gagggggagt atctaaaagt ggagtagaaa aagctcttga agttgtgggg
     1681 ctgcataaag agaaaagaaa agtcttttcc gattattccc tcggaatgaa gcaacggctt
     1741 ggtattgccg ccgccattat gcacgagccg gagcttttaa tacttgacga accgattaac
     1801 ggacttgacc ccattggaat agttgaaata cgctcatttt tatctgaact tagtcacaat
     1861 catggcatta ccatttttat ctcaagccat gttttaagcg agattgaaca gatagcagat
     1921 attatcggcg ttatgcacga ggggcattta gtagaggaag tgaatatatc cgagcttcac
     1981 aaaaggaatc gaaaatacat tcaatttgat ttatctgaca gtgagatagc tggaaagatt
     2041 ttagaaagcc actaccacat gacggatttt acagtgcagg acggtacggt gagaatttat
     2101 gactttagcc aaagtgttgg agaaatcaat cgagaatttg cacgaaatgg actgctcgtg
     2161 acaaggatag atgatagtga ggaaaactta gaggactact tttctaaact gattggaggt
     2221 ggcggtattg cttaatctta tttcttgtga attattaaaa ctaaagcgtt caaaaatggt
     2281 gctaattagt gtggcagggg tattatctac cccacttttg atgttaatag aagccttgca
     2341 aacacatttt gataagccgg agattatctt taccttgtct gacatataca gcgacagtgt
     2401 actgtatatc atgctgctgg taaacattat gatatatgtg gcaattgcag cttacttgtt
     2461 tagcagagag tacacagaaa gcacactcaa aaccatattg cccataccca tttcaaggac
     2521 aaaactatta attggaaaat tttgcaccct gcttctttgg attgttatgc tcacccttgt
     2581 aacatgggca ggtatattta tcgcttgtgg gctatatcac gctgtcttta cattggaggg
     2641 atatagctta ctagtggcaa tagtatggct ccccaagttt ttgtttggca gtatcctgat
     2701 gtttttaaca gtttctcctt ttgtgtttgt tgctatgaaa acaaaaggat ttgtcgcccc
     2761 gatgattggc tctgccgtga ttgtcatggg aagtgccgca ctttcaaatc aagaatgggg
     2821 agcgttgtat ccgtggacag ccacctattt cttggtgcag ggtaaacttc agagtaccgg
     2881 ctatcctaca ctgctgtctg tatctattat tattcttgta tcagcagttg gttttcttat
     2941 gacctttcat cattttaaaa aggaggattt gaaataatgg tacttgattt tatagaaatt
     3001 ttaaaagtta tttttcttgg aattgttgag ggtattactg agtggctacc tatcagcagc
     3061 acaggacata tgattcttgc ggacaaattt atcacactta atatgagcga agcatttaaa
     3121 gaaatgtttt ttgttgttat tcaacttgga gctatactcg cagtggttgt aatgttttgg
     3181 aacaagatgt ttccatttca gctaaaggat aaatcgcagc cacttgttaa aaaggatact
     3241 ttctcgttgt ggttcaaggt tgcagtggct tgtataccgt caggtattat ggggatttta
     3301 tttgatgatt atttggatgc ttacctccaa acccccattg tgatttcaat catgttaatc
     3361 ttttatggtt tgttattcat tctcatagaa aattggaata aaaagcgtac tcctactact
     3421 atggcacttt ctgacatcag ctataaaaca gcaatcttga taggggcatt tcaagtgtta
     3481 tcacttatac ccggcacatc aaggtcggga gcaacgatta taggtgcttt actcatagga
     3541 gtttcacgag tggcagcagc agagtttact tttttcctcg cagtacctac tatgcttgga
     3601 gcaagtgctt ttaagctgtt aaaattcggg tttgatttta caagtgcaga actgctcgct
     3661 cttgttctcg gtatggctgt ggcatttgcg gtatctgtct tagtaattaa attcctaatg
     3721 aactttatca aaaaacatga ttttaaggtg tttggttggt atcgaattgt gcttggtata
     3781 cttgttgtac ttataacagt aatgtgatag aaaaacaatt ccataagcaa taatctgccg
     3841 cacgacggca aaagaaaaaa gccgtcgtac agcagacata ttttcacgcc tatgaaacgg
     3901 tggctttgat tttcagagcc gccgtttctt tgcgtttaca caaaccaatg ggttctgttg
     3961 caaagtctca aaaaggtcat tagtttttca
//
LOCUS       DAUCLR010000210         2246 bp    DNA     linear   BCT 01-JAN-2024
DEFINITION  TPA_asm: Streptococcus pyogenes strain TG265504
            SAMN37144563-rid19226623.guided.137, whole genome shotgun sequence.
ACCESSION   DAUCLR010000210 DAUCLR010000000
VERSION     DAUCLR010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37144563
            Sequence Read Archive: SRR25756717
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 24-AUG-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001860595.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001860595.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/24/2023 19:35:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,875
            CDSs (total)                      :: 1,845
            Genes (coding)                    :: 1,782
            CDSs (with protein)               :: 1,782
            Genes (RNA)                       :: 30
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 23
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 36 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37144563-rid19226623.guided.137"
                     /strain="TG265504"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: ARIZONA,Maricopa"
                     /collection_date="2020-02-22"
                     /collected_by="Arizona State Public Health Laboratory"
     gene            14..1933
                     /gene="tet(M)"
                     /locus_tag="VGT87_001874"
     CDS             14..1933
                     /gene="tet(M)"
                     /locus_tag="VGT87_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEQ3814678.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2052..2219
                     /locus_tag="VGT87_001875"
     CDS             2052..2219
                     /locus_tag="VGT87_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEQ3814679.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
BASE COUNT          800 a          339 c          446 g          661 t
ORIGIN      
        1 ggaggaaaat cacatgaaaa ttattaatat tggagtttta gctcatgttg atgcaggaaa
       61 aactacctta acagaaagct tattatataa cagtggagcg attacagaat taggaagcgt
      121 ggacaaaggt acaacgagga cggataatac gcttttagaa cgtcagagag gaattacaat
      181 tcagacagga ataacctctt ttcagtggga aaatacgaag gtgaacatca tagacacgcc
      241 aggacatatg gatttcttag cagaagtata tcgttcatta tcagttttag atggggcaat
      301 tctactgatt tctgcaaaag atggcgtaca agcacaaact cgtatattat ttcatgcact
      361 taggaaaatg gggattccca caatcttttt tatcaataag attgaccaaa atggaattga
      421 tttatcaacg gtttatcagg atattaaaga gaaactttct gccgaaattg taatcaaaca
      481 gaaggtagaa ctgtatccta atatgtgtgt gacgaacttt accgaatctg aacaatggga
      541 tacggtaata gagggaaacg atgacctttt agagaaatat atgtccggta aatcattaga
      601 agcattggaa ctcgaacaag aggaaagcat aagatttcat aattgttccc tgttccctgt
      661 ttatcacgga agtgcaaaaa acaatatagg gattgataac cttatagaag tgattacgaa
      721 taaattttat tcatcaacac atcgaggtcc gtctgaactt tgcggaaatg ttttcaaaat
      781 tgaatataca aaaaaaagac aacgtcttgc atatatacgc ctttatagtg gagtactaca
      841 tttacgagat tcggttagag tatcagaaaa agaaaaaata aaagttacag aaatgtatac
      901 ttcaataaat ggtgaattat gtaagattga tagagcttat tctggagaaa ttgttatttt
      961 gcaaaatgag tttttgaagt taaatagtgt tcttggagat acaaaactat tgccacagag
     1021 aaaaaagatt gaaaatccgc accctctact acaaacaact gttgaaccga gtaaacctga
     1081 acagagagaa atgttgcttg atgccctttt ggaaatctca gatagtgatc cgcttctacg
     1141 atattacgtg gattctacga cacatgaaat tatactttct ttcttaggga aagtacaaat
     1201 ggaagtgatt agtgcactgt tgcaagaaaa gtatcatgtg gagatagaac taaaagagcc
     1261 tacagtcatt tatatggaga gaccgttaaa aaatgcagaa tataccattc acatcgaagt
     1321 gccgccaaat cctttctggg cttccattgg tttatctgta tcaccgcttc cgttgggaag
     1381 tggaatgcag tatgagagct cggtttctct tggatactta aatcaatcat ttcaaaatgc
     1441 agttatggaa gggatacgct atggttgcga acaaggatta tatggttgga atgtgacgga
     1501 ctgtaaaatc tgttttaagt atggcttata ctatagccct gttagtaccc cagcagattt
     1561 tcgaatgctt gctcctattg tattggaaca agtcttaaaa aaagctggaa cagaattgtt
     1621 agagccatat cttagtttta aaatttatgc gccacaggaa tatctttcac gagcatacaa
     1681 cgatgctcct aaatattgtg cgaacatcgt agacactcaa ttgaaaaata atgaggtcat
     1741 tcttagtgga gaaatccctg ctcgatgtat tcaagaatat cgtagtgatt taactttctt
     1801 tacaaatgga cgtagtgttt gtttaacaga gttaaaaggg taccatgtta ctaccggtga
     1861 acctgtttgc cagccccgtc gtccaaatag tcgtatagat aaagtacgat atatgttcaa
     1921 taaaataact tagtgtattt tatgttgtta tataaatatg gtttcttgtt aaataagatg
     1981 aaatattttt taataaagat ttgaattaaa gtgtaaagga ggagatagtt attataaact
     2041 acaagtggat attgtgtcct gtatgtggaa ataaaacacg attaaagata agggaagata
     2101 ctgaattaaa aaaattcccc ctctattgtc cgaaatgcag acaagaaaat ttaattgaaa
     2161 taaagcagtt caaagtaact gtgattacag agccagacgc aaagacgcag agccgataaa
     2221 atgagattaa tacaatctca ttttat
//
